LOCUS       DAOSII010000001        55595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_670_length_55595_cov_50.964638, whole genome shotgun sequence.
ACCESSION   DAOSII010000001 DAOSII010000000
VERSION     DAOSII010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 55595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55595
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_670_length_55595_cov_50.964638"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="PLV90_00005"
     CDS             complement(<1..1238)
                     /locus_tag="PLV90_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOF95521.1"
                     /translation="MLSLMLVGVVSASYPMFHCDAQRTGYVTQDGPQTNATLWVAETA
                     EYADGSPAVHNGKVFAPTWPDMDFADNDPMGLVCYDAATGTELWTNELGGTSIGSVSG
                     VAVADGQVYLGGTDGRLYCIDEEAGETLWTSGQIDATGYFGLSSSPLVYEGTVYALSA
                     SDGVLHAFTPEGTGSWSFSTGGAVGYFTSPAAAGGKIFVAGGGNNLFCIDAATHTAAW
                     SAALPAAVKSTPVIGDGKVYATTAERLYALDASTGAEVWNASLGGTSSTPAVAGETVI
                     AGSSDGLHAYDAGTGILLWDFPGAQVGVSPVVAGNLVYAATNERTGTVYAVDTRTGEE
                     VWSYTLEAPGDGTFAAFYASSPAVSDGVLYIGAENNRLYAFGDGGLPEPAFTGWSGTV
                     MLTAGRTFTVTPFNNESATYT"
     gene            complement(1556..2635)
                     /locus_tag="PLV90_00010"
     CDS             complement(1556..2635)
                     /locus_tag="PLV90_00010"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HOF95522.1"
                     /translation="MMRNRLLALALAAFIVSIVVVGAAGASHSDSDGVREPTPVPAAD
                     GARTVTVTDDAGKTVTIRGIPQRIVSLAPSNTEILYALGLEDRIAAVTERCDYPPATD
                     GKPKVGGFSTVNIERVIAIEPDLIFAAPANTEEVIDRLRSLGMTVVILDPQTIDDVLH
                     DIELAGRATGQEEEALMLIDGLRKRIENVTEIAAESRAAHPSVAHVIWHDPLWISGAR
                     TFQDEVIRMAGGTNAFGSIDGWSIVSLEEFITTNPDYILVSSGSGMDRDEYDAIYNYI
                     VHEPRLQRLDAVREGRIYVIDADVVSRGSPRIVDALEEVAGYLHPDTAGAGTPEAAGT
                     VQSPGFGAIVLIGALFIVFLIRTKE"
     gene            2875..3057
                     /locus_tag="PLV90_00015"
     CDS             2875..3057
                     /locus_tag="PLV90_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95523.1"
                     /translation="MGFASLAPEQVKKVQRCEEELGVTLLAYKRPTYSELNEENLRRI
                     QALEKELNLSLVAYDV"
     gene            complement(3098..4342)
                     /locus_tag="PLV90_00020"
     CDS             complement(3098..4342)
                     /locus_tag="PLV90_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF95524.1"
                     /translation="MKPIEIIDVDVSYGAKKILEAITFHAVRGEILGIVGPNGSGKTT
                     LLKAMSRVVARDNGEIRLDEQGLDTLGHRELARRIAVVPQDISIGFDYTVRDVVMMGR
                     HPHIGRFASETARDLEICDRAMRLANVADLAEASVHDISGGERQRVLIARALAQEPKI
                     LLLDEATSNLDISHQIEILNIIRGLTGTITVISVFHDLNLAAYYCDRLIMLKEKRVVA
                     VGEPRYVLTRENLNAVYGIDTLVKTHPLTGRPYILPVYGRAAKDRLYENVHVVCGGGT
                     GSDLLYALREAGFRVSTGVLNVFDTDYATATALGIPCVTEAPFAAISPGTREDLAQCI
                     DAAYAVVVTAMPIGQGNIENIRILEDYPDKPVILLNSGGNCLFPDYTGGEAEAIVRRL
                     LDRGAIPVERIEEVLQALTSRA"
     gene            complement(4339..5283)
                     /locus_tag="PLV90_00025"
     CDS             complement(4339..5283)
                     /locus_tag="PLV90_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOF95525.1"
                     /translation="MRRTTPIVILALACTLLISMACAVVLGPVSPGTLVTLPSAWMIV
                     WDVRVPRVIAAALVGCGLAVAGTAMQGLFRNPMADPYIIGTSSGGALGATLAIVLFAG
                     TGRPMFAFAGAMAATFTVYFIARKGGRIPVETLLLSGVALATLLTALLSFLMYTSGRS
                     LHQIMFWLMGGFWNISWNDVFIALPILVGGVGIYLFARDLNILALNEEDAIHLGVNVE
                     RAKQILLALSAFVTGIAVAVAGSIGFIGLITPHVMRLIVGPDHRFLFPAAALAGAILL
                     VWADTLTRTFTGDMPVGILTACFGAPFFIYLLRSRMKA"
     gene            5553..6518
                     /locus_tag="PLV90_00030"
     CDS             5553..6518
                     /locus_tag="PLV90_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HOF95526.1"
                     /translation="MGNLNIAVLGPLGYAKDLGKKGTTSDITFYNLKKGDDTVTLIEP
                     TRYPERLAPLFYAASMADAALVVIGEITPTLGEWILMLDEARVEQGSIVLQNYLTPRD
                     LAPLLRGTVLERYEFVDADPIVLRDRLLACAHARPSVAPAPGVVGTVPIDHHFNVRGI
                     GTVVLGGVTRGGIRKHDILKVYPGEQAITVRSIQKHDDDFDWATEGDRVGLALKNIES
                     DDLDRGFVLSNDPEIRARTNLVARAALVKYWPAPLAAGTVLHVGHWMQFIPARVEAVQ
                     DGGDRRQPTLTLALEKDLVYLPGDTAVLHYLEGGKLRIVGHIELA"
     gene            complement(6637..7674)
                     /gene="mtrH"
                     /locus_tag="PLV90_00035"
     CDS             complement(6637..7674)
                     /gene="mtrH"
                     /locus_tag="PLV90_00035"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit H"
                     /protein_id="HOF95527.1"
                     /translation="MFKFEKEQTVHDFNGTMIGGQPGEYPTVLGASIFYNKHEIVLDD
                     HTGKIDKPTAEALWNRCQELSDLTGIPHFIQIIGEFGEAFESYIDWFCGIDDKTAFLM
                     DSSVPAALAHACKYVTEIGVADRAIYNSINGSIGPDNIEALAKSDVNAAIVLAFNPAD
                     PSVSGREKVLVEGGVAGQELGMLAIAEKCGITRPILDTAATPLGLGSGGAYREILACK
                     AIHGLPTGGAYHNMTVSWTWLKRWKGTKKVPSQQLAGLEGKENLVKQLTHHYLGGTDG
                     LRQAAWSAPDIGCNMIASTLGADLIMYGPIENVEAMITAQAYTDIVVLEAARDFGIEP
                     KVDTHPLFKLV"
     gene            complement(7690..8427)
                     /gene="mtrA"
                     /locus_tag="PLV90_00040"
     CDS             complement(7690..8427)
                     /gene="mtrA"
                     /locus_tag="PLV90_00040"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit A"
                     /protein_id="HOF95528.1"
                     /translation="MAEKKSPASGWPIVQGDFHTGDAQSCVGVVTMGSHLDEQGICDA
                     GAAIAGSCKTENLGLEKVIANVISNPNIRFILCCGTEVKGHLSGQSFIALHAGGVSGG
                     KIVGAEGAIPFIENLSDEAIKRFQEQTEIVNIMESEDMGTIKAKINELKARDPGAFPA
                     EPMVVEVKEAAGAAEVAVAGANPQFLEIEERLNAIEERIEFVDAEIAQRVGRKIGRDI
                     GILYGLVAGLIVFMMLLVLLPKLVGYL"
     gene            complement(8429..8638)
                     /locus_tag="PLV90_00045"
     CDS             complement(8429..8638)
                     /locus_tag="PLV90_00045"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit F"
                     /protein_id="HOF95529.1"
                     /translation="MAEESKQAGPIRMTSINRMMDSIRYKAQILARTTKLESGIMGMG
                     ILGFAIGIAVVMLLTLIPALLMGAI"
     gene            complement(8644..9366)
                     /gene="mtrA"
                     /locus_tag="PLV90_00050"
     CDS             complement(8644..9366)
                     /gene="mtrA"
                     /locus_tag="PLV90_00050"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit A"
                     /protein_id="HOF95530.1"
                     /translation="MVEKKSPASGWPIVQGDFHTGDAQSCVGVVTMGSHLDEQGICDA
                     GAAIAGSCKTENLGLEKVIANVISNPNIRFILCCGTEVKGHLSGQSFIALHAGGVSGG
                     KIVGAEGAIPFIENLSDEAIKRFQEQVEIVNIMESEDMGTIKAKINELKARDPGAFGA
                     EPMIVEVKEAAGAAEEMTGEVQPLSGELALIHARMKIIERMVTDIGYRNKFAAGVYSG
                     KVEGVMIGLIVSFVILGFILLG"
     gene            complement(9377..9661)
                     /gene="mtrB"
                     /locus_tag="PLV90_00055"
     CDS             complement(9377..9661)
                     /gene="mtrB"
                     /locus_tag="PLV90_00055"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019386 - methanogenesis, from carbon
                     dioxide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit B"
                     /protein_id="HOF95531.1"
                     /translation="MAYIQVLPEFGLVVDPMVGVVTTAGVSYTPVLEQVAELEKITDD
                     LVGMLSGEGNFLASFPNREGILNVAGSVTAFWYGMAIGLLVAGVVVLGLL"
     gene            complement(9662..10513)
                     /gene="mtrC"
                     /locus_tag="PLV90_00060"
     CDS             complement(9662..10513)
                     /gene="mtrC"
                     /locus_tag="PLV90_00060"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019386 - methanogenesis, from carbon
                     dioxide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit C"
                     /protein_id="HOF95532.1"
                     /translation="MSVKIEVGAGGIPHNQILIYGLVGSIVLTYLTYLNTITQTQYFA
                     FFGGLAAVAALIWGTDTIKHLCSYGLGTGVPSAGMIALGSGVIAALVGATTGIFAPIV
                     AVIVAAIIGAVAGVLANHVVRMDIPVMIVSLIELAIVGAMTVLGLAAMVGGTFNFTDL
                     VTGTTTFLGFTIQSYEASVIGGSIIAVIFMLAAIAIQHSFNACLGPGEKQDRTLMLAA
                     ECGFLSMIPVAIISFAFIAFIPAIISLVISVVGWFYTYAQYIELSKRDAYAWLDAKPI
                     LEPKGGA"
     gene            complement(10514..11365)
                     /gene="mtrD"
                     /locus_tag="PLV90_00065"
     CDS             complement(10514..11365)
                     /gene="mtrD"
                     /locus_tag="PLV90_00065"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0019386 - methanogenesis, from carbon
                     dioxide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit D"
                     /protein_id="HOF95533.1"
                     /translation="MSALGGPKQTGGIQPPTGTGLVVSIVLIIIALALAFFLVQMAGL
                     IALIGIIIGGVLIGFGVHFVPVGGAPAAMGQAPGIATGVAMLAAGAGLAGLFGGAWAV
                     PLGIPVALAGGAVGGGLLMAITCTMVNVIYVFGMGIPAASGKVAKDPITGDTFPEYKS
                     QGTEGHGLPFISWVGGVIGGALGGFGGTLIYIELLEVYQAQLPALLGATVEQIAPVAV
                     SLAGIFAIGMFLMNAVLAAYNITGTIEGPHDPKFKRFPRAVIAAAAASAVAGIFAIAL
                     SGLLGVF"
     gene            complement(11362..12261)
                     /gene="mtrE"
                     /locus_tag="PLV90_00070"
     CDS             complement(11362..12261)
                     /gene="mtrE"
                     /locus_tag="PLV90_00070"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0019386 - methanogenesis, from carbon
                     dioxide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit E"
                     /protein_id="HOF95534.1"
                     /translation="MEEIVFGIGITALAGALATVAGAAEDTESDIGSQGDPNSQVQLA
                     PQMGYIHRIFNKAIAGEPPAYGLWVALGAGLAWAFMAMHINPILAIVLGSALAVFVQG
                     VYATTAYLGRTASLAKFGQPVYIDILKSMTTVTMAHAFIAIFATVAMCHLMISALGHP
                     FPLPLLGLVWGIALGAAGSATGNPFYGKERQYQEQKFGAGVPISASGNIVRYAEAGQR
                     NSLDNGFFSAKLGGPASGVCFGLIVFFELWRTVVFEPFAAGWGAVVIGVVVILVFAVI
                     DRYIEVWARKTFGPYTVPEEASS"
     gene            complement(12457..14163)
                     /gene="mcrA"
                     /locus_tag="PLV90_00075"
     CDS             complement(12457..14163)
                     /gene="mcrA"
                     /locus_tag="PLV90_00075"
                     /EC_number="2.8.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme-B sulfoethylthiotransferase subunit
                     alpha"
                     /protein_id="HOF95535.1"
                     /translation="MAKIERTQKLFLKSLKEKFQGQDVQSETTEFYKFNGYHQSPRKE
                     EFVKASRAVEMDRGISMYDPVRCHLGGIPLGQRQLMTYEVSGTGVFVEGDDLHFVNNS
                     AMQQMWDDIRRTVIVGMDLAHQTLQKRLGKEVTPETINEYLHVLNHAMPGAAVVQEHM
                     VETHPGLVDDCYVKVFTGDQELADDLEPQFVLDVEKLFPAKQAQALSAAVGKSLWQAI
                     HIPTTVSRTCDGGTTSRWSAMQIGMSFIAAYRMCAGEAAVADLSYAAKHAGVVQMASH
                     LPARRARGPNEPGGIMFGVFSDIVQANRKYPNDPARASLEVVGAGTMLFDQIWLGSYM
                     SGGVGFTQYATAAYTDNILDEYTYYGMDYIKDKYKVDWRNPSPSDKVKPTQEIVNDIA
                     TEVCLNGMEQYEQFPTLMEDHFGGSQRAGVLAAACGLSGSIATGNSNAGLNAWYLCML
                     LHKDGWSRLGFFGYDLQDQCGSANSLSIRGDEGAIGEVRGPNYPNYAMNVGHQGEYAA
                     IVGGAHYGRGDAFCFDPRVKICFADPALKFDFAEPRREFAKGAIREFMPAGERSLIIP
                     AR"
     gene            complement(14166..14930)
                     /gene="mcrG"
                     /locus_tag="PLV90_00080"
     CDS             complement(14166..14930)
                     /gene="mcrG"
                     /locus_tag="PLV90_00080"
                     /EC_number="2.8.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme-B sulfoethylthiotransferase subunit
                     gamma"
                     /protein_id="HOF95536.1"
                     /translation="MAYKPQYGPGTSKVAENRRKQMNPNQKLEKMRDVTDEDIVLILG
                     HRAPGAAYPTAHPPLAEQQEPDCPIRKIVTPTDGAKAGDRVRYIQFTDSMFFAPCHPY
                     QRTYTECYRFRGIDPGTLSGRQIVECRERDLEKYSKELVNTEILDAARTGIRGATVHG
                     HSLRLAENGMMFDMLQRSVLGEDGVVRYVKNQIGEPLDRAVAVGKPLDEKWLKAHTTI
                     FHSLGDVAYRDDKEYIEYVQRIHSLRTKYGFLPKEA"
     gene            complement(14945..15568)
                     /gene="mcrC"
                     /locus_tag="PLV90_00085"
     CDS             complement(14945..15568)
                     /gene="mcrC"
                     /locus_tag="PLV90_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-coenzyme M reductase I operon protein C"
                     /protein_id="HOF95537.1"
                     /translation="MPIGRVTQVVDCRESMGMGKGGGLAQRGTISEARSPDVIVIGMS
                     PGRRHVTKPVCDITSGLRREGAQFSVTTLVLNAGSGVPADSPVAGHVLGAYFGLTEKE
                     IAQIEQHKVAILHHGNVRSHVVQKVRFILEHANIRAIVVSQVPIDFEDLAKEGVRTAV
                     VMPPPDKVKTKGMVMDIVSGVTRGQTPGREKLADVIRAVMKVLKSPT"
     gene            complement(15570..16046)
                     /gene="mcrD"
                     /locus_tag="PLV90_00090"
     CDS             complement(15570..16046)
                     /gene="mcrD"
                     /locus_tag="PLV90_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-coenzyme M reductase operon protein D"
                     /protein_id="HOF95538.1"
                     /translation="MTEAIYPQVRIVSARFLRPDTAEQLLNRLVQVGGILRMSINGPS
                     IPATVPYGPARGKPNPHPDRKVIHVGDQEVLLQVQVGTIILELEDESYITVIREAVDE
                     VFADKDFSCAVQQGRYMKTQPTVSDYAKYGPDADREILGLVDPRKKGGPVIIQGNK"
     gene            complement(16063..17367)
                     /gene="mcrB"
                     /locus_tag="PLV90_00095"
     CDS             complement(16063..17367)
                     /gene="mcrB"
                     /locus_tag="PLV90_00095"
                     /EC_number="2.8.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme-B sulfoethylthiotransferase subunit
                     beta"
                     /protein_id="HOF95539.1"
                     /translation="MAKYKETIDLYDDAGKLLKSGVPLEKISPVVNPATRKLIDLTKR
                     TIAVNLGGVQEGLKTGKVAKGQVLGRELNLDIVGNKDAVIAKIKDMVQVTEGDDTNIR
                     EFGGGKLILVEVPKARLEAASTYDAAITAVASAATYAIVEQFNVGMFDAAMVKAALWG
                     SYPHTMDLTGANVTSILSIPQNNEGLGYALRNIPVNHAVMITNKNAMQGAALSSTFEQ
                     AGMFEMGNAIGPFERAQLLAYAYQGLNANNMVYDLVKKNGATGTIGTVVQSLVERAIE
                     DKVITPGKKGGYFQFYDTKDPMLWNAYAAAGTMAATMVNCGAGRFAQAVSSTLLYFND
                     LLEHETGLPGCDYGRVMGVAVGFSFFSHSIYGGGGPGVFNGNHVVTRHAAGVAIPCVV
                     AAAALDAGTQMFSPESTSKVYGDTFGKVDVFNKPIQQIAQGV"
     gene            complement(17738..18544)
                     /locus_tag="PLV90_00100"
     CDS             complement(17738..18544)
                     /locus_tag="PLV90_00100"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HOF95540.1"
                     /translation="METVTLTIKNQPNLYLEADNITPDAFAGKKAEEIADLHVYVGKE
                     QHRLGEFFEVSGRAGATPGETKIVVNGDLSRVKYIGMKMTAGEVVVNGNADMYVGAWM
                     QGGKITVNGNVDAFAGTGMKGGELLVKGSAGNYLGAAYRGDWRGMQGGKIVVSGNAGS
                     DLGTFMNGGEIVVGGDVDVHVATHAEGGKIVIKGNAKSRLGGQMVEGEIYVFGKIDVM
                     MPGFAYREDVDIEVDGTKGRFALYEGDIGERHRKRKGQLIYGKLYQRIGA"
     gene            complement(18557..20266)
                     /locus_tag="PLV90_00105"
     CDS             complement(18557..20266)
                     /locus_tag="PLV90_00105"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HOF95541.1"
                     /translation="MAEYLIKNGFVFDPVLGIKGDKADVAIKDGKIVETNALRAPEVI
                     DAAGKTVMAGGVDIHAHVAGPKVNIGRNFRPEDKLFTYKPGRGIERMQGGFSVPTTIR
                     TGYNYARMGYTTVMEAAMPPLYARHTHEEMRDTPILDQGAYPVFGNNWFVLEYLKNHE
                     IENTAAYIAWLLRATKGYAVKVVNPGGTEAWGWGLNCENVHDPVPYFDITPAQIVKGL
                     IEANEYLGLPHSMHVHANNLGNPGNYTTTLDTFKLSEGIKPNSKFGRDQVMHHTHVQF
                     HSYGGENWGNMESKATEIMDYVNSHDNITIDIGQVTLDETTTMTADGPFEHHLTELNH
                     LKWANSDVELETAAGVVPYVYSPGIKVCAIQWCIGLELGLLAKDPMRVFISTDHPNAG
                     PFIRYPRVIKWLMSQEAREQQFNAFKHKDKVIEATNLAGIDRELDLYEVAQMTRAGPA
                     KALGLAHMYGGLAPGMEADVAIYAFNPNAPYAPDDIETAFLNAEHLFKTGVQVIRDHE
                     IASNGNKRTLWVNARVNENPQVMRDVKEKFLRYYTVTLNNYEVTGHHYLPNPYVIEVD
                     ATQ"
     gene            complement(20270..21583)
                     /locus_tag="PLV90_00110"
     CDS             complement(20270..21583)
                     /locus_tag="PLV90_00110"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HOF95542.1"
                     /translation="MTKTVTDVVCPFCGTLCDDLEVVVSDDGKKLFEVYNACAIGAEK
                     FLHSQAADRLTRPRMRQDDGSWKEISYDEAIEYTARMLADAKKPLMYGWSSTNCEAQA
                     VGSEIGEMVGAVMDNTATVCHGTSLIAVQDIGIPSCTLGEVKNRADRILFWGCNPAHA
                     HPRHMSRYSIFPRGFFTGKGHTGRKMIVVDPRPTDTARIADVHLQIEQGRDYELLDAF
                     RVAFKGEKLPDVVAGIPKEKIYEAADMLKSGRFAIIFFGMGVTQSLGKNHNIDAAIAV
                     TRDLNEYTKAAIMPMRGHYNVTGSGQVWGWLFGFPYAVDLSRGFARYNPGETTSNDLL
                     RRGEVDAVFVLGSDPGAHFPFSSVKEIYNLPSVAVEPHETPTTEVCKVHVPVAFVGVE
                     VGGCAYRMDNVPIETRKVVEPPEGMLTDEEFLDRVLARVKEIKGV"
     gene            complement(21580..21981)
                     /locus_tag="PLV90_00115"
     CDS             complement(21580..21981)
                     /locus_tag="PLV90_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HOF95543.1"
                     /translation="MAIRVNLITGRTIQQGVSMEAGKEKPAYTAACGIIELDPADFKK
                     LGAFRNTTVRVTSQYGSVVVKAVEATQGPHPGVAYIPMGPWANRVINPNTYSTGMPTF
                     KGTPVTVEVAKNEPVLDSLELVRKGCRGELA"
     gene            complement(22009..22434)
                     /locus_tag="PLV90_00120"
     CDS             complement(22009..22434)
                     /locus_tag="PLV90_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOF95544.1"
                     /translation="MADENWKPKIIAIICNWCSYAGADLAGGARIQYPPDIRAVRVMC
                     TGRVDPLFILKAFQDGADGVLVSGCHFGDCHYLEGNYKAAKRMFLLKSVLKNIGLDDK
                     RLRMTFVSASEGAKWGHVMEDVVRTITELGPSPLKELAR"
     gene            complement(22435..24459)
                     /locus_tag="PLV90_00125"
     CDS             complement(22435..24459)
                     /locus_tag="PLV90_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOF95545.1"
                     /translation="MAEVKKYEEPRIGVFVCHCGTNIAGSINIKAVQEYAKTLPNVII
                     ADDYQYMCSTPGQNKIAEAIKEQKLTGIVVAACSPRLHEPTFRNATKEGGLNPFRFEM
                     ANIREQNSWVHMHQPEEATAKAKDAVRIAVAKASLLEDLIPKSVPVEKTAMVVGAGVG
                     GMQAALDLASAGIKTYLVEKEPTIGGKMSQLDKTFPTLDCSQCILTPKMVDVYRHEGI
                     ELLTYTEVEAVEGYIGNFDVTLRKKARGVLTPDEATARGIIGGGCTGCGDCTDVCPVI
                     KPNPFELGMAPRKAIYIYHPQVSPLIYTVDFDACVKCGLCVEACGEERKAIDLDAKDE
                     LMNVKVGTVVLALGYDIFPIEKKFEWGYKHYDNVITSLEFERLICASGPTGGHLIRPS
                     DGETPKRIAFVLCAGSRDNTGVGKPYCSRFCCMYSLKHAHQIIEKIPGAIPYIFYMDI
                     RSFGKMYEEFYYRIQDEGAKFIRGRVANILEDPVTKNLHVNAEDTLLGRPIDMEVDLV
                     VLAAAVQPSADTEKTRRLFGVSCSQDGWLLEAHPKLNPCGTTTAGVYLAGVCQGPKDI
                     PDTVAQAEGAASAASIPIHRGEVELEPYFAVCMEDKCAGCGLCVNLCPYQALSLIEKD
                     GRTVMHVTEAKCKGCGTCGGFCPGGAIWMQHFATPQIVAQIDAFLLGGEQ"
     gene            complement(24471..25370)
                     /gene="hdrB"
                     /locus_tag="PLV90_00130"
     CDS             complement(24471..25370)
                     /gene="hdrB"
                     /locus_tag="PLV90_00130"
                     /EC_number="1.8.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase subunit B"
                     /protein_id="HOF95546.1"
                     /translation="MSNRHQYAFFLGCIAPNRYPGIEAAAIKTSANVGIDLLPLKGAS
                     CCPAPGAFGSIDLNVWYAMAARNVVLAEQMKTDITLICNGCYKSIWEVNHKLKHNDEL
                     RDGVNEVLKEIDMEFKGTVDVWHLAELYYDPKIVGIKKLADSVTRPLTGAKIAVHYGC
                     HLMKPAKERHFGDTENPMWIEELVAALGAEPVQYRNKMQCCGAGGGVRGYDIAHALDI
                     TNEKMINLQEVGADALTEVCPFCQLQYDRGQIEIEEKFGITWGLPILHYNELLGLAQG
                     MSPDELALDLHAVSVEPFLKKIL"
     gene            complement(25375..25956)
                     /gene="hdrC"
                     /locus_tag="PLV90_00135"
     CDS             complement(25375..25956)
                     /gene="hdrC"
                     /locus_tag="PLV90_00135"
                     /EC_number="1.8.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase subunit C"
                     /protein_id="HOF95547.1"
                     /translation="MAVKKDYKDQKLAERLRDRKYYIPDSNPEFIKDVEKIGRTAAHM
                     CYQCGTCTGSCPSAPRSSYRIRLFMRKAILGLEEEALTDPDLWLCTTCYSCTDRCPRD
                     LAPTDAIMAMRNLAFKRDIVPRNFLKTAQLIYQSGHGVPNNAANRVAREKLGLEAEPE
                     TTHKYPEYLPGIRKIMDHYRLKENADRILAEGE"
     gene            complement(25967..27136)
                     /locus_tag="PLV90_00140"
     CDS             complement(25967..27136)
                     /locus_tag="PLV90_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOF95548.1"
                     /translation="MALFPKYSKTREGQNVIMEQKLLKAVNNLILNAETCTGCGICVD
                     ACPEEAIVLGPVGATRRGAIDYAEPVDVNPEKCSYCGVCVIMCPFNAMTLKIDGEERL
                     PILEKEGFPQYDMVTVIDEEKCDRCTICEEVCPRDAINRDVPAFEGGEEAGRPRETAL
                     QTKTTFKVDMEKCSVCGICGELCPSITVVRKPRTPETGKIEGDVLWEESTCDGCKICV
                     EACPEDAITLEREVVSDRLPGKVDIQQDDCCTCTWCVQTCPTEAITVEKIFEGEIEIN
                     PEKCPGGCSTCVEVCPCNALYLPTPLPAKEMKGRIEPNIAINKDFCIFCGACVNACPS
                     EDAIILRRTAIRMQDKDSELFRDIKEKLLTTRTSKVKETTPGEVEIKVLGKVEEA"
     gene            complement(27152..27424)
                     /locus_tag="PLV90_00145"
     CDS             complement(27152..27424)
                     /locus_tag="PLV90_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOF95549.1"
                     /translation="MAFALHINMERCTGCNNCVVACPVNALELHTEEPVTLEKIYKVK
                     DGKAIILDFNSELCAGCGVCVEACPYEVIKLAGPWESRAKARRVEA"
     gene            28204..29112
                     /gene="fhcD"
                     /locus_tag="PLV90_00150"
     CDS             28204..29112
                     /gene="fhcD"
                     /locus_tag="PLV90_00150"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HOF95550.1"
                     /translation="MELNGVTIDDTYAEAFPTWVARPIITAVTEEWAYRAAVEAVGFA
                     TSTIGCPAEAGIDCFVPADETPDGRPGYAILICASKKKLKEQVVERLAECVLTAPTTA
                     VFDGLADVVAEVQEKLPVKLHFFGDGYEQKREVGGRTVWAIPIMEGEYIGEEEFGAVK
                     GVAGGNFFIMGENQMAALMAAQAAVDAISGVCGVITPFPGGIVASGSKVGSKKYKFMG
                     ASTNEVYCPTLRDKVEGSKVPEGVKAVYEIVIDGVDQDSVRTAMAEGIRAASKVPGVK
                     FISAGNFGGSLGPFRFELKDVLADYL"
     gene            29405..30361
                     /locus_tag="PLV90_00155"
     CDS             29405..30361
                     /locus_tag="PLV90_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Ile2) 2-agmatinylcytidine synthetase"
                     /protein_id="HOF95551.1"
                     /translation="MITLTPDDVRARFGPLFSMKYLAMVDQNAGLAEIREHCRARGTI
                     EWDAANRIRAGGAIRSCHVEGTTMTMLARLGLSPAKFGAAGREIGGQALEGVEVDGDE
                     VVTTWSGIAGAGVGVAACLTQAPGVIRAEYPSEDDLRIGGARVCRVRIVSPLYEKVTI
                     GIDDTDTREEGATWVLALKCAEACTIPGVEYLDMRLVQLNPAVPKKTTNCVGSALNFA
                     VRPGRVDALLEYVRDFIESEAVSKDTGIAVYRGIAFAEESSYARRVKTELLTLEEAEA
                     EAARMGVRFIDSNRRKGRIGALGAVLWGNKGVEAAGLYGETF"
     gene            30345..31238
                     /locus_tag="PLV90_00160"
     CDS             30345..31238
                     /locus_tag="PLV90_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1743 domain-containing protein"
                     /protein_id="HOF95552.1"
                     /translation="MERLSDPYTIRYPQIVAVADESGGHVELIEFFDCTGGAMWVKRH
                     YAQSPLVRSVRTVGATNRYILSAGSADLALEGSVFPAGIAAVAVQGDEIAVTYRGLGG
                     GGVGASICRASASGVVRHKSDPAGGGRLAGSTIWLPRRERVVIGIDDTDTPEEGATWT
                     LAHNIARAVEDDRSRYLSHTIVQLYPVPYRTKNCVAIACEFATSDPGGLARHYRDLLT
                     EYTLSEETGMAVWRGFDTAPLEEFGGKVKRGEVSADDLAAITDGRLSVLMDGRGAIGA
                     VAAIPFFTRYEEALALWNGSG"
     gene            31220..32188
                     /locus_tag="PLV90_00165"
     CDS             31220..32188
                     /locus_tag="PLV90_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOF95553.1"
                     /translation="MEWVRLKARLLEAGSVRLSGEPADQYISRSAAGPSAGAPGSLFF
                     SLGDRRVRVGIDETAPIEVIHRGEGVADLLIGGDMVRGRLEAAALHCPRQAYVTVSGT
                     CIFHCRYCTVPHLPGRRKGIAEVVGMVESVADRIDAIAITSGVASSIEEEEAYVLDVV
                     AALAPFGLPIGVSIYPGPETPARLRALGVVEVKFNLEAATETLFRDMCPGLSWEAVHE
                     ALFRSVALFGRGRVFSNVIVGLGETDGDLERVMTDLAAAGVIPVLRPLTPAASLAGRP
                     RPPADRLLRLSAAHERILREAGLDPRRALTMCPACTGCDLVPGRDT"
     gene            32185..33441
                     /locus_tag="PLV90_00170"
     CDS             32185..33441
                     /locus_tag="PLV90_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOF95554.1"
                     /translation="MKGEEALARAILRSADRCYTVPGYPVSDLAGRVGAAITANEKVA
                     VEYALGDSLSGRRAAVIVKHVGLNACADPLVHATAQGLRAGVVIVVGDDLRAAASDVV
                     QDSRYYGEVARVPVLEPDGETIGPAVEAAFEASETFSRIAIVRVTPGLLDADVPETPA
                     PRSGREGSLADPGLTMAGRAQAADRRTAAMVAWSRTSPLNRYEGDVFHAVTVYPPPAD
                     PDVLARILEAGRPFLREHRLLEPPATTGEPERFSARGYSRTFCRDCPFHPALAILSER
                     RMRTTCDAGCAILAMNPPYRVGLATYGLGSSVAVAATGPGIALAGDYALLHSGLAALI
                     DVYERGLPLLCIVLANNRMGMTGGHPVPDILRYIAWADPVVCAADDTGALRRALTLPE
                     EGPRTVVIKGTCPEGAHHETLEYRDL"
     gene            33413..34543
                     /locus_tag="PLV90_00175"
     CDS             33413..34543
                     /locus_tag="PLV90_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase family protein"
                     /protein_id="HOF95555.1"
                     /translation="MKPWNIEICDVTLRDGEQTPGVSFTHDEKMTIARALDEIGVEVI
                     EAGFPVVSSAEKECVAAIARSGLSARVCCLARALRPDVEAALDCDVDMVSIFIATSDL
                     HIRHKYRKPRAEVLDDALDMVEFAADHGVQVRFAAEDASRTDPAFLIEMYSRGAECGA
                     DLVSFADTVGCLAPLEIHAVISRILGDVHHPLCIHCHNDLGFAAANTITAAAAGAFQL
                     HTTVNGIGERAGNAALEQVLVALRMKGGVDRYDLTRLQEISRLVARCSGVVPERTRPI
                     VGENAFAHESGIHIAAILEDPATYESILPELVGGERRFVLGKHTGRKALEHIAQAYGF
                     DLSDGQVRWVLEQIKQRSEGKCSVTPEVLCGFLRQAKAEVLS"
     gene            34540..35751
                     /locus_tag="PLV90_00180"
     CDS             34540..35751
                     /locus_tag="PLV90_00180"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HOF95556.1"
                     /translation="MSTLSERILGAPAGAYVDREVDIAFAHDGTGVLTREALREMGVE
                     QLPHPERLRLVFDHIVPANTGTTATLQAELRGYARASGIDLSDAGGGICHQVMSEGVV
                     RPGMVVVGADSHTCTLGALGAFATGVGATDMAAIWASGATWFRVPETIAVNLTGRLGG
                     PAEPKDVALACVARLGMEGATYRALEFVGDGASGISMDGRLTLCNMAVETGAKTGIFY
                     ADAVTVRHLAGHGVAASPQAVEEPRYEREIAIDLSGIVPLVAVPHRVDTVREAEDVAG
                     TPIDQVFVGTCTNGRYEDLARLARIVRGRKVAVRTLVVPASRAVLARAIATGVLADIV
                     DAGCMVAPPGCGPCLGAHEGVMGEGEVCLSTANRNFKNRMGVGAEIYLSSVATAAVSA
                     LAGEIAVPEVA"
     gene            35751..36221
                     /locus_tag="PLV90_00185"
     CDS             35751..36221
                     /locus_tag="PLV90_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HOF95557.1"
                     /translation="MQGTGPAVCLGNDVDTDLIIAGRYLRTKDRSVWAEHVLEDLDPA
                     LAPRIRGAIIVAGRNMGCGSSREQAVVALREAGVVGIVAESFARIFFRNAVNVGLPVI
                     EASVACTDGAHVAFDLDSGWVEVDGVRRPARPLSPRMVTILRAGGLVPYWRSCR"
     gene            36218..37042
                     /locus_tag="PLV90_00190"
     CDS             36218..37042
                     /locus_tag="PLV90_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95558.1"
                     /translation="MIFPPHCKYVGAVTGTPCGERVYFLSRYLVRETEPDGMEVLEVE
                     ADPAGTGLMRSILSTRVLAAGDEVCRYPDPVNVQDRTFLVRAALRSGHRCTVFSGHGC
                     QTTFVLDPDLSGFLRLHVYDITPPRPHLSATLGDLERTGLFGDLEVIFEHHVRDIREI
                     EADVYPCRAAGFPRTLDADPVRPGDRVAGCLTAKDLVRECYGEEIGVESICPLGAVTA
                     EPFVARCCRSERGGLGRWNGKFGAVVHWGASSWEIADAVRQVAAAWRERNGEGSGR"
     gene            37020..37991
                     /locus_tag="PLV90_00195"
     CDS             37020..37991
                     /locus_tag="PLV90_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOF95559.1"
                     /translation="MVKVAVVEGDGIGREVVPAARDILAAVRPDIEFFDVDLGYGLWE
                     RTGCACGEETVADLGSADAILFGAVTTPPDPGYRSVMLQIRHALDLYANVRPIRGDTV
                     DVVIVRENTEGLYSGIEWTEPDRSCTVRVVSRRGSERIARYACTLAKSRRHHLTVGNK
                     ANVLKSDCLFVEVCTAVAAHLGVPCTARYIDALSLDLLMHPDRYDVIVTTNMFGDILS
                     DAAAYLVGGLGMLPSANIGERAALFEPVHGSAPDIAGRNVANPIAAIRSGAMLLRHVG
                     DPASAAAVEEAVVRVLRAGIRTRDIGGTAGTREFGAAVLREVGRGKA"
     gene            38017..38865
                     /locus_tag="PLV90_00200"
     CDS             38017..38865
                     /locus_tag="PLV90_00200"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOF95560.1"
                     /translation="MVLVGCHVSIAGSIDLAVGRALDSGCDTFQIFSRNPRGWKAKDI
                     DPDVAGAFRRALDASGIGPAVDHMPYLPNLASPDDAIYEKSVAALTDELRRCEVLGIP
                     YLVTHLGHHRGAGTGTGQERVVAAINRALEEAGAQGVMLLLENTAGEKNSVGSTVADL
                     CRIFEGIDGKERIGICFDTCHAFAAGYDLRTGEGVDAVLEEIDDLIGPGHLRIIHLND
                     CKGDLGSGLDRHEHIGLGSIGEEGFRHILSRPAIRGCPLICETPVDGRRGDDGNIAKV
                     RELAGA"
     gene            39156..42326
                     /locus_tag="PLV90_00205"
     CDS             39156..42326
                     /locus_tag="PLV90_00205"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOF95561.1"
                     /translation="MIVCTVIAAGLLASPVLAVEAAPGETVLFCTAGGSQEHPDIDGD
                     MVVWEDGRDDRSIYYAAAPGSGGRKVAGGYAEERYPSVSGDFIAWQDRRHGNWEIYLF
                     SPYDGERRITNSTTDQTMPIVHGNHIAWYDTRKGYTDICLYDIATGKETYLGCSPRIE
                     WKLALSERYVVWEEIAGGIQAYDIQTEEKLGIAASGARQAYPAISGDRIVWEDYRNGL
                     PDIYMYDLNEGREYQITDDPAEQVSPAIDGEIIAWEDKRSGIWDIYMYDLRAWTEMSV
                     CTANGEQLFPSVSGEKIVWQDKRSGRWQVYLFAYSGGAPPEAEFSADPIAGTVPLTVR
                     FADRSTGSPDEWEWEFGDGGSSTEPNPGHTYEDPGEYTVSLRVSNRFGSDIVTKTDLI
                     RAKTLEPPAADFTAAPVSGSLPLTVRFADRSTGAPSAWLWDFGDGATSNEQDPEHTYA
                     AVGTYAVSLTAGNPAGSTNMTKPAFITVVSPPKASFSADTRSGRAPLAVRFSDASTGT
                     ITAWSWDFGDGGSSHDQNPTHTYTGPGIYTVALTAANEYDSDTRTMEGYITVTAPPVA
                     GFSANVTAGRSPLAVKFSDESGGGPLSWSWTFGDGASSAEKSPVHVYQAPGAYTVTLT
                     VANGAGENTTARTGYITVRDLLSARFTANVTAGAAPLAVLFTDASVGEPRSWSWDFGD
                     KGTSTVPAPVHVFEQPGNYTVSLTIADGADRSTKSLQIRAFGPPGAEFSAAPLNGTAP
                     LTVAFTDASSGEPSAWLWDFGDQGRSIERHPSHTYTAAGNYTVNLTVENAAGTDTATK
                     TGYVSVHPAPVTPAPTSGGGGGGGGGGGGGGGGGGGGSTWTTPDPGTPTATATPTATP
                     SPGPAGPDVLPLGESGLVSRFVRIASPDGVASLAIAEGVRAVDAVGGPLRTVTLKLLD
                     SPDVPGAPGSYVFSGYACTAGPDGAAFSPPATLVFNLTEEEWDAVYSSRRGFLVQWYN
                     RSANAWEEVPTLVHPETRSVTAEVSHFSLYALFVGLPGDGAVEVIAPDTPQAGEAFPS
                     MQIIPGLIALAIVGAGAYLYYRRGEP"
     gene            complement(42371..43525)
                     /locus_tag="PLV90_00210"
     CDS             complement(42371..43525)
                     /locus_tag="PLV90_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95562.1"
                     /translation="MAALLIAVAMIAVVPVSGAALPVAWNATFSPEENSKFDAVAGTA
                     DGGYIALGSVLAGSGEDLLLVKTDGRGSEVWTERFPGMAAASVAETADGGYIAAGYNA
                     TAEPEQNAYQGSSLLIRTDAAGKEEWRQVLQGEKVSAVRPTADGGYVIAGWLWDPPGS
                     ANDTTAIVTKTDGSGTPTWNRTFPGAAANAGIVTADGGYIIGGTKSPFAYDIGDAFLI
                     RLDTAGNTLWEKNYAVPVIFDVEETDEGGFVHSGNFWYGLVDAKGDEVWLRNMEGLAG
                     YAVTLRPAGGYMIAGTDIRRGEAFAFGTDGDGAIRWNTTIPDTRVYAAESTPGGYVLA
                     GIRHLSPASGAAWLAGLEEAAEPAPTTPGFGAIAAGAALLLLFAGRKRRG"
     gene            43700..44710
                     /gene="endA"
                     /locus_tag="PLV90_00215"
     CDS             43700..44710
                     /gene="endA"
                     /locus_tag="PLV90_00215"
                     /EC_number="4.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000214 - tRNA-intron endonuclease
                     complex [Evidence IEA];
                     GO_function: GO:0000213 - tRNA-intron endonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-intron lyase"
                     /protein_id="HOF95563.1"
                     /translation="MLATFDGTWVRLGGEGRTLYEQGGYGRPEGTGLRLSPEEALYLM
                     ERGKIDVKDFDFDALLGLFAGQPNFVRRYLVYRDIRERGYVVQPGPHDFRVFRRGHKP
                     GVGRSQYLIRVLSERDLVDFEQLGQDVLAAVNMRKQYLLAVVDDEDELTYYEVRVQDL
                     PGVGEPARCDRPIEASLFGTYALAHLPPGTPLEEGWYGKRLDPERLLFRPVESIYLMQ
                     KGCLAVTRDGGRMTAEEFRGVAAEKDIEIREKERVFSDLREKGYIPRTGYKFGHHFRV
                     YSGKKTHSEMLVHAVPSRTSTPMSAVSRSVRLAHSVKKKMLFACIYNSDIGYIEFARI
                     KL"
     gene            44721..45977
                     /locus_tag="PLV90_00220"
     CDS             44721..45977
                     /locus_tag="PLV90_00220"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOF95564.1"
                     /translation="MQSGMNPWSSNQTVDVDRLFAEFGIEPVGEVARGLPEVPPFMRR
                     GVVVGHRDYGLIADAIRNRTPFHVLTGFMPSGLPHLGHLMVMKEVVWHVRQGGNGYVA
                     IADREAHAVRGISWEKCREYGKEYLKALYALGFCGTTYYQSRNDRLKDLAFEASTKVN
                     FSELTAIYGFGPETSLSHAVSVVTQVADILFPQLDAGPAPTVVPVGLDQDPHIRLTRD
                     VAYKLRMFTVEDRGDHISVRSKNAPEAALEAVHRTFSGSKKYAGHVDVTGVPMARIED
                     AVRGIEIARGGFGFYLPSSTYHIFMPGLQGGKMSSSVPESLFGFYEPEPSVRKKIMGA
                     LTGGRMTLEEQRRLGGEPGGCPVFLLNLFHMLEDDRELADLRRRCEGGELTCGQCKKE
                     TYERVQAFLRELRDKMDAVEHLAEEV"
     gene            45981..47426
                     /locus_tag="PLV90_00225"
     CDS             45981..47426
                     /locus_tag="PLV90_00225"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOF95565.1"
                     /translation="MELTQNEKRLLAALGPVGSADAAALAGMMDARREAVVQYANLAG
                     ERGLVDVEKRISRRYVPTDEGRACAGKGLPERQVFESFGEAIPMRDLQAHPLAKIAVG
                     WMRKKGWVTIRDGVVQKTGNAAPGPDETVFARISENGSAEDGPGVADLLRRGLVEEEE
                     TVTYTVSITPLGCSLLAGGLDLEEEAGTLTRDQILSGAWRDLPLRRYDVTKLPRRAYP
                     GKVHPYQRLLDEMRRILLDMGFTEMAGGIVQSSFWNFDALFQPQDHPAREMQDTFFLG
                     ERWPLPAGYERVRDMHEHGGETSSAGWGGTWSAAKAEQCVLRTHTTGLSIQHLAKHPK
                     PPVRAFCIGRVYRREAVDPTHLAEFEQLEGIVMDEGVNFRHLLGFLKEFYAKMGFEKV
                     RFRPGYFPYTEPSVEPEVYVENLGWVELGGAGIFRQEVTAPFGIEHPVLAWGLGISRV
                     AMLRLGLRDLRHLYRSDVEWIRETPTYGGRR"
     gene            47428..49068
                     /gene="pheT"
                     /locus_tag="PLV90_00230"
     CDS             47428..49068
                     /gene="pheT"
                     /locus_tag="PLV90_00230"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOF95566.1"
                     /translation="MAIITLPYRYLERLTGTDRQTIIDRVPMIGADIERIEEDHVDVE
                     FFPDRPDLYSPEGVARAMRGFLGIEEGLPVYTVHPSGITFSVDPGLAGIRPHLASAVI
                     RNVDLDEEAIESLMSLQEALHWAVGRGRAKVAIGVHDLDMVTPPFRYIASPRSRSFVP
                     LDFDREMTMEEILVDHPKGRDYARLVEDFPRFPLIVDAEDRVLSFPPIINGELTRVTA
                     ATKNILLDCTGTDRKAVMTATNIICTALAEAGATIESVTVAGEEMPTLAPAERIVSVD
                     ECAALLGIPLTGEEMVRLLRRMRFGAEPEGASHLRVFVPCYRSDILHDWDIFEDVAIA
                     YGFENFDAALPPTSTTAEEHPVTAIAGAVRSVMIGLGYLEMMPFTLTNERVLYANMQR
                     EPLPGTLRVLHPISEEQTVVRTDLLPLLMEMLQANKHRELPQRLFAVGDVVRYCVTSQ
                     MAAAVSIHPAADFSEAYAAADVLCRELALPYTVAESSDPAFIDGRRGDIIVDGKIVGV
                     FGEIHPAALNAFDLEHSVAAFALDLTAVPGYPVPPGTP"
     gene            complement(49025..49831)
                     /gene="hisF"
                     /locus_tag="PLV90_00235"
     CDS             complement(49025..49831)
                     /gene="hisF"
                     /locus_tag="PLV90_00235"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOF95567.1"
                     /translation="MVPTKRIIPCLDLKDGRVVKGTHFTDLCDAGDPVALARRYDEQG
                     ADELVFLDIAASKEHRGTIIDVIRRASDQLALPFTVGGGIRTIDDMQQILGAGADKVS
                     INSSAVENPELISRGAERFGAQAIVVAIDVRRNYAVEPGKKPIALADGRECWYEVVTH
                     GGSRATGRDAVAWAREAEERGAGEILLTSMETDGTKDGFDIPITAAVATAVGIPVVAS
                     GGVGTLEHFYEGFAEGKADACLAASVFHYGVFAIREVKEYLAGRGIPVRR"
     gene            50049..51275
                     /locus_tag="PLV90_00240"
     CDS             50049..51275
                     /locus_tag="PLV90_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOF95568.1"
                     /translation="MESGDLKIAWARQYMPVLSSIRERFIAEKPFAGMTIGMALHVEA
                     KTAVLVETLAAGGAEVYITGCNPLSTQDDVSAALNTRKGVHSYARRGADVEEYYAAID
                     RVLDARPAVTIDDGMDLIYRIHTERREVLDAVIGGCEETTTGIHRLRAMARDGALRFP
                     VIAVNDTPMKHFFDNVHGTGESSLASIMITTNVLIAGKHFVVAGYGYCGRGLAQKARS
                     LGARVIVTEVDPRRALQAHMDGFDVMTMDDAAPLGDIFVTTTGNAGIITGRHFPNLKD
                     GAILANAGHFNVEIDVEWLASHADSTVHRDGIDTYVLGGRAIHVLAEGRLVNLATPKG
                     MGHPIEVMDLSFAIQALSAEFIAKRGRDLAPGVHDVPAVIDEGVARLKLAALGLSIDR
                     LTPEQETYMSSWTIGT"
     gene            51862..52143
                     /locus_tag="PLV90_00245"
     CDS             51862..52143
                     /locus_tag="PLV90_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95569.1"
                     /translation="MGLSDSVSSKQVGVRLPGHLYRWLKNKVDSGEYSNMAQSVIGEL
                     TKVKTLEEARCQETTSHGTYDDEPLSRMVNERIEGVRRELLDEVKRRRA"
     gene            52733..53560
                     /gene="modA"
                     /locus_tag="PLV90_00250"
     CDS             52733..53560
                     /gene="modA"
                     /locus_tag="PLV90_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOF95570.1"
                     /translation="MRSSIPATLVAVSLLVIAGLLTAGCTTTEETPGPTTLTVFTAAS
                     LTGAFTDIGRAYEAQNADIKVDFVFDGSQTLRTQIEQGATPDIFVSASTKHMKALQDG
                     GFMDNSTVATFLGNSLALIVPVDNPAGITSLADLDEPGVKLVIGTKDVPFGDYTRQMF
                     EKMAADPAYGQAYQDAVMKNVVSEEMAVSSVVPKLALGEADAAIVYKSDVSKDDLTKV
                     TRIGIPAEYNVVATYPLGVLAESPSKAEAESFIAFVRGPDGSAVLTDYGFDPIPAGN"
     gene            53529..54371
                     /locus_tag="PLV90_00255"
     CDS             53529..54371
                     /locus_tag="PLV90_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF95571.1"
                     /translation="MDLTRFRQETNVFEYGSRTLLRRATLTLLTAVLLALFLLFITLP
                     VVSLFLRISPEAFLRSLAEPVVLDALSLSFITATVSTAIVVVFGTPLALVNARSDYAG
                     RELVDTLTDLPIVLPPAVAGLALLMAFGRRGVIGQYLDIFGIHIGFTTLAVVMAQVFV
                     ASPFYIRQARASFEAVDRLYEDAARTLGASRRTVALRITIPLAWGGLVSGAILSFARA
                     LGEFGATIMFAGNFQGRTQTMPLAIYTTMQGDMDASISLAIILVVISFAVIAAVKVVT
                     RRSA"
     gene            54371..55084
                     /locus_tag="PLV90_00260"
     CDS             54371..55084
                     /locus_tag="PLV90_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF95572.1"
                     /translation="MLSIHVGRQLRDFAIDVALSVRCGETLVLIGENGAGKSTVLNLI
                     SGILAPDRGDITLGERTLFSSAARIDIPAESRNIGHLFQSYALFPHMTVAENVAFGLR
                     CRKVPKPEIAGAVAEQLRSMHLSDLADVNVGRLSGGQRQRVALARALVLEPELLLLDE
                     PLAAVDMRARGAMREELRDRIRHAGIPCIVVTHTLHDVLGLADRLCLIEEGRVATEGT
                     PEEVLGMRENGFIASFIEG"
BASE COUNT         9769 a        17439 c        17636 g        10751 t
ORIGIN      
        1 gtgtaggtgg cgctctcgtt gttgaagggg gtgaccgtga aggtccggcc ggccgtgagc
       61 atcaccgttc cgctccaccc ggtaaacgca ggttccggta gtcctccatc cccgaaggcg
      121 taaaggcggt tgttctcggc accgatgtag agaacgccgt ccgataccgc gggggaggag
      181 gcatagaacg cggcaaacgt gccgtcaccc ggggcttcga gggtgtatga ccagacctct
      241 tcgccggtcc tggtatccac cgcatacacg gtgccggtcc tctcgttcgt cgcggcgtag
      301 acgaggttcc cggccacgac cggggagacg cccacctgcg cacccgggaa atcccagaga
      361 aggatcccgg tcccggcgtc gtaggcgtgg aggccgtcgg aagaccctgc gatgacggtt
      421 tcacctgcaa cggccggcgt cgaggatgtc ccgccgaggc tcgcgttcca gacctcagcg
      481 cccgtgctcg catcgagggc gtagaggcgc tcggccgtcg tcgcatagac cttcccatcg
      541 ccgatgaccg gtgtcgactt cacggcggcc gggagggcgg cgctccacgc ggcggtgtgg
      601 gtggcggcat cgatgcagaa gaggttgttt ccgccccctg cgacgaagat cttcccgccc
      661 gcggctgcag gcgaggtgaa gtagccgacg gccccaccgg tggaaaaaga ccatgatccg
      721 gtgccttcag gcgtgaaagc atgcagcacg ccgtccgaag ctgagagggc gtagaccgtt
      781 ccttcgtaga cgaggggcga tgacgagagc ccgaagtagc cggtggcgtc gatctggccg
      841 ctcgtccaga gcgtctcccc cgcctcttca tcgatgcagt agagccgtcc gtcggtcccg
      901 ccgaggtaga cctgaccgtc ggcgacggca acgcccgaga ccgaaccgat ggaggtgccg
      961 ccgagttcgt tcgtccagag ttccgtcccg gtggcggcgt catagcagac aagccccatc
     1021 gggtcgttgt cagcgaagtc catatccggc caggtcgggg cgaagacctt tccgttatgc
     1081 accgccggtg atccgtcggc atattcggcg gtctctgcca cccagagggt ggcgttcgtc
     1141 tgcggcccgt cctgcgtgac gtagcccgtc cgttgcgcgt cgcagtgaaa catcggataa
     1201 gatgcactca ccacgccgac gagcatgagt gagagcatga taagcccaac aaagagtggc
     1261 agctgccttg ttctcatgtg tacaagtaca cttgttatag tgtaataaaa ctttacaaaa
     1321 cgattttgat ataattaaaa attatttgtt tttttttcga tcgaccgctc cccgggtgct
     1381 cgacatgccg gtacgctgtt tccggcccga tcaagcggtt tactgtcaaa aaacggagtg
     1441 atatcgtcga acccgccggt ccgggtggcg gcgcgcagcg aggttgccgc gaagagcgta
     1501 accggcatac agagccgcgt gccgcgcctg ttctgcaata cgtgtcgtta ctgccttatt
     1561 ctttcgttct gatcaggaag acgatgaaca gcgcaccgat gagaactatc gcgccgaacc
     1621 ccggcgactg gaccgtcccg gccgcctccg gcgtacctgc cccggcggta tccgggtgga
     1681 ggtagcccgc tacttcttcc agcgcatcca cgatccgcgg gctcccgcgg ctgacgacat
     1741 ccgcgtcgat gacgtagata cggccctccc tgaccgcatc gagcctttgc aggcgaggtt
     1801 cgtgtacgat gtagttgtag atggcgtcgt attcatctct atccatgccg ctccccgaac
     1861 tgaccaggat gtagtcgggg ttcgtggtga tgaactcctc gaggctgacg atgctccagc
     1921 cgtcgatcga gccgaacgca ttggttccgc cggccatcct gatcacttcg tcctggaacg
     1981 ttctggcgcc gctgatccag agcggatcgt gccagatgac gtgggcgacg gacggatgcg
     2041 cggcccggct ctcggccgct atctcggtaa cgttctcgat gcgcttccgg agtccgtcga
     2101 tgagcatcaa agcctcttcc tcctgcccgg tcgcccttcc ggcaagttcg atgtcgtgca
     2161 ggacatcgtc gatcgtctgc gggtcgagga tgacgacggt cataccaagc gatcgcaggc
     2221 ggtcgatgac ctcttcggtg ttggcgggcg cggcgaagat caggtcgggt tctatggcga
     2281 tcaccctctc gatattgacg gtgctgaacc cgcccacctt cggtttaccg tctgttgccg
     2341 gaggatagtc gcagcgctcg gtgacggcgg cgatccggtc ctcaaggccg agggcataga
     2401 ggatctctgt gttcgagggc gcaagcgaga cgatcctctg tggtatcccc cggatggtga
     2461 cggtctttcc ggcatcatcg gtcaccgtga cggttctcgc gccgtctgct gccggtaccg
     2521 gggtcggttc ccggacgccg tcggaatccg aatgcgatgc gcctgctgcg ccgacaacga
     2581 cgatcgatac gatgaacgcc gcgagagcga gtgcgagcaa tctgttcctc atcattgcaa
     2641 gtatatcgat acagaatgat aatacgttac cccgtgcggc ctgatacggt caccggagag
     2701 gttgtgcacc cccgggggcc catgcccggc cggaatccgg cgttgcgggg gcgggaccaa
     2761 acctccccct ccggatctct ccaccccaac ggttacggcc ggagcaccgg cggactccat
     2821 aacctatatg tgcgtgacgg gcctgtaatg ctccgggtga gaaccgtgag cactatggga
     2881 tttgcgagtc ttgcgcccga gcaggtgaag aaggtgcagc gttgcgaaga agaactcggt
     2941 gtaacgctcc ttgcgtataa gagacccacg tactcggagt tgaacgagga gaacctcagg
     3001 cggattcagg ccctcgagaa ggagctgaac ctctcgctcg tggcctacga tgtctgatcg
     3061 gagcctctac ctcgtatttt tgtcatgttc tggcgggtca ggcccggctc gttagagcct
     3121 gcaggacctc ttcgatccgc tccaccggga tcgccccccg gtcaagcagc ctccggacga
     3181 tcgcttccgc ctcaccgccg gtgtaatccg ggaagaggca gttgccgccg ctgttcagga
     3241 ggatgacggg tttatccggg taatcttcca gtatcctgat gttctcgatg ttcccctgcc
     3301 cgatgggcat cgccgtgacg acgacggcat atgcggcatc gatgcactgc gcgaggtcct
     3361 cgcgggtgcc gggagatata gcggcaaagg gcgcttcggt aacacaggga atgcccagtg
     3421 cggttgcggt tgcgtagtcg gtatcaaaga cgttgagcac gcccgtgctt acccggaacc
     3481 ccgcctcgcg gagggcgtag aggaggtcgg aacccgtacc gccgccgcat acgacgtgca
     3541 cgttctcgta gagcctatct tttgcagcac gcccgtacac cgggaggatg tagggtcttc
     3601 cggtcagggg atgggtcttt accagggtat cgatcccgta gaccgcattg aggttttcac
     3661 gggtcagcac ataccggggt tcgccgacgg cgacgaccct cttctctttg agcatgatca
     3721 gccggtcgca gtagtatgcg gcgaggttca ggtcgtgaaa gacgctgatc accgtgatcg
     3781 taccggtgag gcccctgatg atgttgagga tctcgatctg gtggcttata tcgaggttgg
     3841 aggtcgcctc gtcgagcagc aggatcttcg gttcctgcgc gagcgcacgc gcgatgagca
     3901 cgcgctggcg ctccccgccg ctgatgtcgt gcaccgaggc ttcggcgagg tccgcgacgt
     3961 ttgcaagacg catggcacgg tcgcagatct ccaggtcccg ggcggtctcg gaggcgaacc
     4021 gcccgatgtg gggatgtctc cccatcatga cgacatcgcg caccgtatag tcgaatccaa
     4081 tcgatatatc ctgcgggacg accgcaatcc ggcgtgcaag ctcgcggtgt ccgagcgtat
     4141 ccagaccctg ctcatcaagc cggatctctc cgttgtcccg ggcgacgacc cggctcatcg
     4201 ccttcaagag cgtcgtcttc cccgagccgt tcggcccgac gatgccgagg atctcacccc
     4261 tcaccgcatg gaacgtgatc gcttcaagga tcttctttgc gccgtaggag acgtcgacgt
     4321 cgatgatctc aatcggtttc atgctttcat ccggctccgg aggaggtata tgaagaacgg
     4381 tgcgccaaag caggcggtca gaatgccgac gggcatgtcg cccgtgaagg tccgcgtgag
     4441 cgtatccgcc catacgagga ggatggcgcc cgcaagcgca gctgcgggga agagaaaacg
     4501 gtggtcgggc ccgacgatca ggcgcatcac gtgcggggtg atcaggccga tgaacccgat
     4561 cgacccggct acggcaacgg ctatccccgt cacgaacgcg ctcagcgcga ggaggatctg
     4621 ttttgcccgc tcgacgttca ccccgaggtg gatcgcgtct tcttcgttca gggcgaggat
     4681 gttgaggtcg cgcgcgaaga gatagatccc cacgccgccg accaggatgg ggagggcgat
     4741 gaagacgtcg ttccacgata tgttccagaa ccctcccatc agccagaaca tgatctggtg
     4801 caggctcctt cctgaggtat acatgagaaa agagaggagc gccgtgagaa gcgtcgcgag
     4861 ggcgacgccg gagaggagaa gtgtctcgac cggtatcctg cctcctttgc gggcgatgaa
     4921 gtagacggtg aacgtggcgg ccatcgcccc ggcaaatgcg aacattgggc gccccgtgcc
     4981 ggcgaagagg acgattgcaa gcgtggcccc gagcgctccg ccggacgacg tgccgatgat
     5041 gtaggggtcg gccatggggt tacgaaagag cccctgcatg gccgtgcctg ctacggcaag
     5101 cccgcacccg accagcgccg cggcgatcac cctcggcacc ctgacatccc agacgatcat
     5161 ccatgcgctg ggcagggtaa ccagggttcc gggggagacc ggtccgagaa cgactgcgca
     5221 ggccatgctg atgaggagcg tgcaggcaag tgccaggatc acgatgggcg ttgttcttcg
     5281 catagggtgt agtaaggtta actttaattt gttaaaatgt atttggttaa cgccgtggtg
     5341 tgcgtggagc ccaccgcaca tccggccgca tctgcgggat ccccgggtat ggtgcaccgc
     5401 atggcggttt catgcgaaag gagtgcggag gcgcgaaggg gggcggtgca ggagtcttca
     5461 ggatcgtggg ttccccgtgc ccgggcacgt tttttccaaa agatcgtgcc tgcgttaacc
     5521 tcatactctc gggtaaccaa atgatcaggc atatgggcaa cctgaatatc gccgtgctgg
     5581 gacctctcgg ctacgccaaa gacctcggga agaaaggcac gacatccgat atcaccttct
     5641 ataacctgaa gaagggcgac gataccgtca ccctcatcga accgacccgg tatcccgaga
     5701 ggctggctcc gctcttctac gccgcatcga tggcggatgc ggctcttgtc gtgatcggtg
     5761 agatcacccc gacgctcggg gagtggatcc tgatgctcga tgaggcccgc gtggaacagg
     5821 gctccatcgt cctccagaac tacctcaccc cccgggacct cgcgccgctc ctccgcggga
     5881 cggtgcttga gcgttacgag ttcgtggatg ccgatccgat cgtgctccgc gaccgcctgc
     5941 tcgcttgcgc gcatgcccgc ccgtccgtcg cacctgcacc cggcgtcgtg ggaacggtcc
     6001 ccatcgatca tcacttcaac gtccgcggca tcgggacggt cgtcctcggc ggcgttacgc
     6061 ggggcggcat ccggaagcac gacatcctga aggtctaccc cggggagcag gcgatcacgg
     6121 tgcggtccat ccagaagcat gacgacgact tcgattgggc aaccgaaggc gaccgcgtgg
     6181 ggcttgcgct caagaacatc gagtccgacg atctcgaccg cggttttgtc ctctcaaacg
     6241 accccgaaat ccgggcccgg acgaacctcg tggcgcgggc ggccctggtc aaatactggc
     6301 cggcacccct tgcggcaggg acggtgctcc acgtcggcca ctggatgcag ttcataccgg
     6361 cgcgggtgga ggcggtgcag gacggcggcg accggcggca accgacgctc acgcttgcgc
     6421 tcgaaaagga tctcgtctac ctccccgggg atacggcggt cctccactac ctcgaggggg
     6481 ggaaactcag gattgtcgga catatcgaac ttgcgtgaag cggggatttt gagaacccta
     6541 acgttttggt aaccccggaa tgaggtgccg atatcgcttc tacccgctcg tctcccctct
     6601 atcgggccgt gcggggctgt aaaaaaaggg ttgggtttag acgagtttga agagcgggtg
     6661 ggtatcgacc ttgggctcga taccgaaatc gcgcgccgct tccagcacga cgatgtcggt
     6721 gtaggcctgt gcggtgatca tcgcttcgac gttctcgatc gggccgtaca tgataaggtc
     6781 ggcgccgagc gtgcttgcaa tcatgttgca cccgatatcg ggggcagacc atgccgcctg
     6841 gcgcagtccg tccgtaccgc cgaggtagtg gtgcgtgagc tgcttgacga ggttttcttt
     6901 gccctcaagg ccggccagct gctgcgaggg gactttcttc gttcccttcc accgcttcag
     6961 ccaggtccag gagaccgtca tgttgtggta tgcaccgccg gtggggaggc cgtggatcgc
     7021 cttgcaggca agaatctcgc ggtacgcgcc gcctgagccg agcccgagcg gggttgccgc
     7081 ggtgtcgagg atcgggcggg tgatgccgca cttctccgcg atcgcaagca taccgagctc
     7141 ctggcccgcg acgccgccct cgaccaggac cttctcacgg ccggagaccg acgggtcggc
     7201 ggggttgaag gcgaggacga tcgcggcatt cacgtcgctc ttggcgagag cctcgatgtt
     7261 atccggtccg atcgaaccgt tgatcgagtt gtagatcgca cggtccgcaa cgcctatctc
     7321 ggtgacgtac ttgcacgcgt gagcaagcgc cgccgggacg gaagagtcca tcaggaacgc
     7381 tgtcttgtcg tcgatgccac agaaccagtc gatgtaactc tcgaaggcct cgccgaactc
     7441 cccgatgatc tggatgaagt gcggaatgcc ggtgaggtcc gagagctcct ggcaacggtt
     7501 ccagagcgcc tccgccgtgg gtttgtcaat ctttccggtg tggtcatcca gcacgatctc
     7561 gtgcttgttg tagaagatgg atgcaccgag cacggtcggg tattctccag gctgcccgcc
     7621 gatcatggta ccgttaaagt cgtgtaccgt ctgctctttt tcgaacttga acatattcgt
     7681 caatcctcct tacaggtacc caactaattt gggcagtaac accaacaaca tcatgaatac
     7741 aatcaaacct gcaaccagtc catacaggat gccgatatcg cgcccgatct tccttccgac
     7801 gcgctgggct atctctgcat cgacaaactc gatcctctct tcgatagcgt tgagccgctc
     7861 ctcgatctca aggaactggg ggtttgcacc tgcaacggca acttccgctg caccggccgc
     7921 ctccttgacc tcgacgacca tgggttctgc ggggaatgca ccggggtccc tggccttgag
     7981 ttcgttgatc ttggccttga tggtacccat gtcctcgctt tccatgatgt tgacgatctc
     8041 ggtctgttcc tggaagcgct tgatggcttc atcggagagg ttctcgatga acgggatggc
     8101 gccctctgct ccgacgatct tgccgccgga gacaccgccg gcatgcagag caatgaaact
     8161 ctggccggag aggtggccct tcacttccgt accgcagcag aggatgaacc tgatgttggg
     8221 gttggagatg acgtttgcga tgaccttctc gaggccgagg ttctcggtct tgcaggaccc
     8281 ggcgattgcc gctccggcat cgcagatacc ctgctcatcg aggtgggatc ccatggtgac
     8341 gacgccgacg caactctgtg catctccggt gtggaagtcg ccctgaacaa tcggccatcc
     8401 gctggccggt gatttcttct cagccatgtt agatcgcccc cataagcagt gccggaatca
     8461 gagtcagaag catgaccacg gcgatcccga ttgcgaaccc gaggatcccc atacccatga
     8521 tgcccgactc gagtttggtc gtgcgagcaa ggatctgtgc cttgtaccgg atggaatcca
     8581 tcattctgtt gatcgaggtc atccggatgg ggcctgcctg tttactttct tctgccatct
     8641 ctatcacccc agcaagataa acccgagaat cacaaacgag acgatcaggc cgatcatgac
     8701 accctcgacc ttgcccgagt agacaccggc agcaaacttg ttgcggtagc cgatatcggt
     8761 gaccatccgc tcgatgatct tcatccgtgc atgtatcagc gcgagctcgc cggagagcgg
     8821 ctgcacctcg ccggtcattt cctctgccgc accggctgcc tccttgacct cgacaatcat
     8881 gggttctgcg ccgaacgcac cgggatctct ggccttgagt tcgttgatct tggccttgat
     8941 ggtgcccatg tcctcgcttt ccatgatgtt gacgatctcg acctgctcct ggaagcgctt
     9001 gatggcttcg tcggagaggt tctcgatgaa cgggatggcg ccctctgctc cgacgatctt
     9061 gccgccggag acaccgccgg catgcagagc aatgaaactc tggccggaga ggtggccctt
     9121 cacttccgta ccgcagcaga ggatgaacct gatgttgggg ttggagatga cgtttgcgat
     9181 gaccttctcg aggccgaggt tctcggtctt gcaggacccg gcgattgccg ctccggcatc
     9241 gcagataccc tgctcatcga ggtgggatcc catggtgacg acgccgacgc aactctgtgc
     9301 atctccggtg tggaagtcgc cctgaacaat cggccatccg ctggccggtg atttcttctc
     9361 aaccatgttc ttcacctcac agcagtccta atacgacgac accggcaacc agaaggccga
     9421 ttgccatgcc ataccagaat gcggtcacgc tcccggcgac attgaggatt ccctccctgt
     9481 tcgggaacga tgccaggaag ttgccttcac cggagagcat accgaccagg tcgtcggtga
     9541 tcttctcgag ttccgctacc tgttcgagca cgggagtgta cgagacgccg gcggtggtga
     9601 cgacaccgac catcgggtcg acgaccagcc cgaactcggg cagtacctga atgtatgcca
     9661 tctaggcacc tcccttgggc tccaggatcg gctttgcatc gagccaggcg taagcatcgc
     9721 gcttcgagag ttcgatgtac tgggcgtagg tgtagaacca cccgacgacc gagatcacga
     9781 gcgatatgat agcggggatg aacgcgatga atgcaaacga tatgatcgcg acggggatca
     9841 tggagaggaa cccgcactct gcggcgagca tgagcgtccg gtcctgtttc tcccccggcc
     9901 cgagacaggc gttgaaggag tgctggatcg cgattgccgc gagcatgaag atcacggcga
     9961 tgatgctgcc gccgatgacg gaggcttcgt aactctgtat ggtaaacccg aggaaggtgg
    10021 tcgtgccggt gacgaggtcc gtgaagttga acgttccgcc gaccatggct gcgaggccga
    10081 gcaccgtcat cgctccgacg atcgcaagct cgatcagaga gacgatcatg accgggatat
    10141 ccatacggac gacgtggttt gccagtaccc cggcgaccgc accgatgatc gcggcgacga
    10201 tgaccgcaac gatgggcgcg aagatgccgg tcgtggctcc tacgagcgcg gcgatgacgc
    10261 cggacccgag tgcgatcata cctgccgacg ggacaccggt accaagaccg taactgcaga
    10321 ggtgcttgat cgtatcggta ccccagatga gcgcggcgac ggcggcaagc ccgccgaaga
    10381 acgcaaagta ctgggtctgg gtgatcgtgt tcaggtatgt caggtaggtc aggacgatcg
    10441 atcccacgag cccgtagatc aggatctggt tgtgcgggat gccgcctgcg ccgacttcaa
    10501 tctttaccga catttagaat acccccagca gtccggacag cgcgatcgca aagatgcctg
    10561 caaccgccga tgctgcagcg gccgcgatga ccgcccgcgg gaatcgcttg aacttggggt
    10621 cgtgcggtcc ctcgatcgta ccggtgatgt tgtacgccgc aagcacggcg ttcatcagga
    10681 acatgccgat cgcgaagatg ccggcaagcg acacggcgac gggtgcgatc tgctcgaccg
    10741 ttgcgcccag gagcgcgggc agctgcgcct ggtagacctc gaggagctcg atgtagatga
    10801 gcgttccgcc aaagccgccg agtgcaccgc cgatgacgcc gccaacccag gagatgaacg
    10861 ggaggccgtg accctcggta ccctggctct tgtactcagg gaaggtgtcg cccgtgatcg
    10921 ggtcctttgc gaccttaccc gaagctgccg gtatacccat gccaaagacg tagatgacgt
    10981 tgaccatcgt acaggtgatc gccatcagaa ggccgccgcc gaccgcaccg ccggcgaggg
    11041 cgacaggaat gccgagcggg acagcccagg cgccaccgaa gagcccggca aggccggcgc
    11101 cggcagcgag catcgcgaca ccggtcgcaa tacccggtgc ctgtcccatt gctgcgggtg
    11161 ctccgccgac cgggacgaag tggacgccga atccgatcag gacgccgccg atgatgatgc
    11221 cgatgagggc gatcagcccg gccatctgta cgaggaagaa agcaagcgca agcgcgatga
    11281 tgataagtac aatactgaca acgaggcccg tccccgttgg gggctggatg cctcccgtct
    11341 gtttcggtcc gccgagtgcg ctcatgacga cgcctcctca ggaaccgtgt agggcccgaa
    11401 ggtcttcctt gcccagacct cgatgtagcg gtcgatgacg gcaaagacca ggattacgac
    11461 gacaccaatg acgacggctc cccatccggc ggcgaacggc tcaaagacca cggtacgcca
    11521 gagttcgaag aagacgatca ggccaaagca gacacccgat gcggggccgc cgagtttggc
    11581 gctgaagaaa ccgttgtcaa gcgagttccg ctgaccggct tcggcgtagc ggacgatgtt
    11641 gccggacgcg gagatcggaa cacccgcgcc gaacttctgc tcctggtact gccgctcctt
    11701 tccgtagaac gggttaccgg ttgcagaccc ggctgcaccg agcgcgatac cccagacgag
    11761 gccgagaagc gggagcggga aggggtggcc gagcgcgctg atcatcaggt gacacatcgc
    11821 gacggtggca aagattgcaa taaacgcgtg tgccatggtc acggtcgtca tcgacttcaa
    11881 gatatcgatg tagaccggct gtccaaactt ggcgagactt gcagtacgac ccagatacgc
    11941 ggttgtcgcg tacacgccct gcacgaagac tgcaagagcg ctcccgagta cgattgcaag
    12001 tatcggattg atatgcatcg ccatgaaagc ccaggcaagg cctgcaccca gagcaaccca
    12061 gaggccgtac gcgggaggtt caccggcaat tgccttgtta aagatgcggt gaatatatcc
    12121 catctgtgga gcaagctgaa cctgtgagtt cgggtcaccc tgtgatccga tatcagactc
    12181 agtatcctcc gcagcgccag ctacggttgc aagagcacct gccaatgcgg taatgccgat
    12241 gccaaatacg atctcttcca ttcagcatcc tcctttgatg cacgtttgca ccagagtatg
    12301 agttcaaaac ctttttggtt catttaacag ttgtttatga cttaattaaa ggtttcgaaa
    12361 agttgcgccg atatagcaac gaaacgttaa aaatcgcccg gaatgctcgt tttaggcata
    12421 taggattgtt ctaaggaaaa aaaagttgaa ttgggtttac cgggccggga tgatgagcga
    12481 acgctcgcca gcgggcatga attcgcggat tgcacccttc gcgaactcgc ggcggggctc
    12541 ggcgaagtcg aacttcaggg ccgggtcggc gaagcagatc ttcacccgcg ggtcgaaaca
    12601 gaacgcatcg ccgcggccgt agtgcgcgcc gccgacaatc gcggcgtact cgccctggtg
    12661 gccgacgttc atcgcgtagt tcgggtagtt cgggccgcgg acctcgccga tcgcgccctc
    12721 gtccccacgg atggagagcg agttcgcgga cccgcactgg tcctggaggt cgtagccgaa
    12781 gaagccgaga cgcgaccatc cgtccttgtg caggagcatg cagaggtacc aggcattcag
    12841 gccggcgttc gagtttccgg ttgcgataga gccggagaga ccgcaggcag cggcaaggac
    12901 accggcacgc tgggaaccgc cgaagtggtc ctccataagg gtcgggaact gctcgtactg
    12961 ctccatgccg ttgagacaga cctcggtcgc gatgtcgttg acgatctcct gggtcggctt
    13021 gaccttgtcg ctcgggctcg ggttccgcca gtcgaccttg tacttgtcct tgatgtagtc
    13081 cataccgtag taggtgtact catcgaggat gttgtcggtg taggccgcgg tcgcgtactg
    13141 ggtgaatccg acaccgccgg acatgtagga gccgagccat atctggtcga acagcatggt
    13201 tccggcgccg acgacctcga gggaggcccg ggcggggtcg ttggggtatt tccggttcgc
    13261 ctggacgata tcggagaaga caccgaacat gatgccgccg ggctcgttcg ggccacgggc
    13321 acgccgggca ggcaggtggg aggccatctg gacgacgccc gcgtgctttg ccgcgtagga
    13381 gaggtcggcg acggccgctt caccggcgca catgcggtag gcggcgatga aggacatacc
    13441 gatctgcatc gcagaccacc gggaggtcgt tccaccgtcg caggtccgtg ataccgtggt
    13501 cgggatgtgg atcgcctgcc agagggactt tcctacagcc gcggagagcg cctgggcctg
    13561 cttcgcgggg aagagcttct cgacgtcgag gacgaactgg ggctcgaggt cgtcggcaag
    13621 ttcctggtcg ccggtgaaga ccttgacgta acagtcatcg acgaggccgg ggtgggtctc
    13681 gaccatgtgt tcctggacga cggccgcgcc gggcatcgcg tggttgagca cgtggaggta
    13741 ctcgttgatc gtctcggggg tgacttcctt accgagacgc ttctgcaggg tctggtgggc
    13801 aaggtccatc ccgacgatga cggttctcct gatatcgtcc cacatctgct gcatagcaga
    13861 gttgttgacg aagtgcaggt cgtcgccctc gacgaagacg ccggtgccgc tgacctcgta
    13921 ggtcatcagc tggcgctggc cgagcgggat accgcccagg tggcagcgca cggggtcgta
    13981 catggatatg ccgcggtcca tctcgacggc gcggctcgcc ttcacgaact cctccttgcg
    14041 gggggactgg tggtagccgt tgaacttgta gaactcggtc gtctcggact ggacatcctg
    14101 cccctggaac ttctccttga gggatttcag gaacagcttc tgggttctct caatctttgc
    14161 catctttatg cctccttcgg aaggaacccg tatttcgtcc tgagcgagtg gatgcgctgg
    14221 acgtactcga tatactcctt gtcgtcacgg taggcgacat cgccgagcga gtggaagatc
    14281 gtcgtgtggg ccttgagcca cttctcgtcg agcggtttgc cgacggcgac cgcacggtcg
    14341 aggggctcgc cgatctggtt cttgacgtaa cggacgacgc cgtcctcgcc aaggacgctc
    14401 ctctggagca tgtcgaacat catgccgttc tcggcaagac ggagcgagtg gccgtgcacg
    14461 gtcgcgccgc ggatgccggt gcgggccgcg tccaggatct cggtgttgac gagttccttt
    14521 gagtacttct cgaggtcacg ctcgcggcac tcgacgatct gacggccgga gagtgttccc
    14581 gggtcgatgc cgcggaagcg gtagcactcg gtgtaggtcc gctggtaggg gtggcagggg
    14641 gcgaagaaca tcgagtccgt aaactggatg tagcggacac ggtcgccggc cttggcaccg
    14701 tctgtcgggg ttacaatctt tctgatggga cagtcgggct cctgctgctc ggcaagcggc
    14761 gggtgggccg ttggataggc ggctcccggc gccctgtggc cgaggatcag gacgatgtcc
    14821 tcatcggtca cgtcacgcat cttttcaagc ttctggttgg ggttcatctg cttgcgccgg
    14881 ttctcggcga ccttggatgt acccggtccg tactggggct tgtatgccat gtttactttc
    14941 accttcatgt tgggcttttt agcactttca taacggcacg aatgacatct gccagttttt
    15001 ccctgccggg tgtctgaccc cgtgtcacgc cgctgacgat atccatcacc atgcctttcg
    15061 tcttcacctt atcaggcggc ggcataacca ctgctgtcct gactccctct tttgcgagat
    15121 cctcgaagtc gatcgggacc tgagatacga cgattgccct gatgttcgca tgctcaagga
    15181 tgaaccggac cttttgcacc acgtgggagc ggacattccc atggtgcagg atggcgacct
    15241 tgtgctgctc gatctgggct atctctttct cggtcaaccc gaagtacgct ccgaggacat
    15301 gacccgcgac gggtgaatct gccggtaccc cgctccctgc attcaggacg agcgtggtca
    15361 cggagaactg tgccccttct ctccggagac cggaggtgat gtcgcagacc ggttttgtga
    15421 cgtgtctgcg gcccggggac atcccgatca cgatcacatc cggggatctg gcttcagaga
    15481 tggtgccccg ctgggcaaga ccgcctcctt ttcccatccc catgctctcg cggcagtcga
    15541 caacctgggt tacccttccg attggcatgt tacttgtttc cctggatgat aacaggcccg
    15601 cctttctttc tgggatcgac aagcccgaga atctctctgt cagcgtcagg tccgtacttc
    15661 gcgtagtcgg acaccgtcgg ctgggtcttc atgtaccgtc cctgctggac ggcgcaggag
    15721 aagtccttat cggcaaagac ttcgtcgact gcctccctta tgaccgtgat gtacgactcg
    15781 tcctcaagct ccaggatgat cgttccgacc tgtacctgga gcaggacttc ctggtctccg
    15841 acatggatga ccttacggtc cgggtgggga ttgggtttcc cccgtgccgg gccataggga
    15901 acggtggcgg ggatgctcgg tccgttgatg gacatccgga gaatcccacc gacctgaaca
    15961 agcctgttca ggagttgttc cgccgtatcg ggtctgagga atcgcgcaga aacgattcga
    16021 acctgagggt atatggcttc tgtcattggt atcctgtgtt atctacacac cctgtgcgat
    16081 ctgctggatc ggcttgttga agacgtcgac cttgccgaac gtgtcgccgt agaccttgga
    16141 ggtgctctcc ggcgagaaca tctgggttcc ggcatcgagt gccgctgcag cgaccacgca
    16201 cgggatggcg acgcctgcgg cgtgcctggt cacgacgtgg ttgccgttga agacaccggg
    16261 gccgccgcca ccgtagatcg agtggctgaa gaacgagaac ccgacggcga cacccatcac
    16321 gcggccgtag tcacagccgg ggaggccggt ctcgtgctcg agcaggtcgt tgaagtagag
    16381 gagcgtcgaa gagactgcct gggcaaaccg cccggcaccg cagttgacca tggtggctgc
    16441 catggttccg gcggcggcgt aggcgttcca gagcatgggg tccttcgtgt cgtagaactg
    16501 gaagtacccg cccttcttcc ccggagtgat gaccttgtcc tcgatggcgc gctcgaccag
    16561 gctctggacg accgtgccga tggttccggt tgcaccgttc ttcttgacga ggtcgtagac
    16621 catgttgttc gcgttcaggc cctggtaggc gtaggcgagc agctgggcac gctcgaacgg
    16681 tccgatggcg ttacccatct caaacattcc tgcctgctca aaggtggacg agagtgcggc
    16741 accctgcatg gcgttcttgt tcgtgatcat cacggcgtgg ttgaccggga tgttacggag
    16801 cgcgtagccg aggccttcgt tgttctgggg aatcgacagg atggacgtga cgttggcacc
    16861 ggtcaggtcc atcgtgtgcg ggtaagagcc ccagagtgca gccttgacca tggccgcatc
    16921 gaacatgccg acgttgaact gctcgacgat cgcgtaggtt gccgcggatg cgaccgcggt
    16981 gatcgctgca tcgtaggtgg atgccgcctc gagacgagcc ttcgggacct cgacgaggat
    17041 gagcttgccg ccaccgaact cgcggatgtt ggtgtcgtcg ccttctgtga cctggaccat
    17101 atccttgatc ttggcgatga cggcatcctt gttcccgacg atgtcgaggt tgagttcgcg
    17161 cccgaggacc tgtcccttcg caacctttcc ggtcttcagg ccttcctgaa cgcctccgag
    17221 attgacggca atcgtcctct tggtaaggtc gatgagcttc ctggtcgccg ggttgaccac
    17281 cgggctgatc ttctcaagcg gcacaccgct cttcaacagc ttgcctgcat catcatacag
    17341 gtcaatagtt tccttgtatt ttgccatgat tttactccat taccctcttt gagtgcatat
    17401 aatctggaac tgtagactga atgctaaaag tatcgctgac tgtgtctggt gataactact
    17461 gcacgtcctc gtgggacaaa aagataataa cctcttctga tgatataagc gttgctattt
    17521 acgtctatgg tcagatatat atccggagca gaatattaga tatccactga atgcctatca
    17581 atcacagtgt atatcggacg tttcatctcc ttcaggagtg aaaaggagtg atcaaaatag
    17641 tataattccg tagtacttta tgttactttt tacctggaga taatatatta cctcgggctg
    17701 gcgaccttat tccgtataca cggcaaaaaa agagggatca ggccccgatc cgctggtaca
    17761 gtttgccgta gatcagttga cccttcctct ttcggtggcg ttcgccgata tcgccctcgt
    17821 agagagcaaa tcgtcctttg gtgccatcta cctcgatatc gacgtcttca cggtatgcaa
    17881 atccgggcat catgacatcg atcttgccaa agacgtagat ctcgccttcc accatctggc
    17941 cgccgagacg gctctttgca ttgcccttga tgacgatctt gccgccctcg gcatgcgtgg
    18001 caacatggac gtcgacgtct ccgccgacga cgatctcgcc tccgttcatg aacgtgccga
    18061 gatcgctccc ggcatttccg gagacaacga tctttccgcc ctgcatgccg cgccagtccc
    18121 cacggtacgc agcgccgagg tagttgcccg cgctgccctt gacgaggagc tcgccgccct
    18181 tcatcccggt cccggcaaag gcgtcgacgt tcccgttcac cgtgatcttg ccgccctgca
    18241 tccatgcacc gacgtacatg tcggcgttgc cgtttacaac gacctcgcct gcggtcatct
    18301 tcatgccgat gtacttgacc cgcgagagat ccccgttgac gacgatcttc gtctcccccg
    18361 gagtcgctcc ggcccggccg gagacctcga agaactctcc gagccggtgc tgttccttgc
    18421 ccacgtacac gtggaggtcg gcaatctcct cagccttctt cccggcaaac gcgtcggggg
    18481 tgatgttatc agcctcgaga tacagattgg gctggttctt tatggtgagt gtaacggtct
    18541 ccatcctttc tgcacctcac tgcgtggcat cgacctcaat cacgtacggg ttcgggaggt
    18601 agtggtgtcc cgtaacctcg tagttgttca gggtgacggt gtagtagcgg aggaacttct
    18661 ccttgacgtc acgcatcacc tgcgggttct cattcaccct ggcgttcacc cagagcgtcc
    18721 gtttgttgcc gttcgaagcg atctcgtggt cgcggatgac ctggacgccg gtcttgaaga
    18781 gatgctcggc gtttaagaat gccgtctcga tatcgtcggg cgcgtagggc gcgttcgggt
    18841 tgaacgcgta gatcgcgaca tccgcttcca tgccgggcgc aagcccgccg tacatgtgcg
    18901 cgaggccgag cgcctttgcg ggccctgcgc gggtcatctg cgcgacctcg tagaggtcga
    18961 gctcgcggtc gataccggca aggttcgtcg cttctatgac cttatccttg tgcttgaagg
    19021 cgttgaactg ctgctcgcgc gcctcctggc tcatgagcca cttgatgacc ctcgggtagc
    19081 ggatgaacgg accggcgttc gggtggtcgg tgctgatgaa gacccgcatg gggtcctttg
    19141 cgagcagtcc gagttcgagg ccgatgcacc actggatggc gcagaccttg atccccgggc
    19201 tgtaaacgta gggcacgaca cccgcggcgg tctcgagttc gacgtcggag tttgcccact
    19261 tcaggtggtt caactccgtg aggtgatgct caaacggccc atcggcggtc atggtcgtgg
    19321 tctcgtcgag cgtcacctga ccgatgtcga tggtgatgtt gtcgtgcgag ttcacgtagt
    19381 ccatgatctc cgttgccttg gactccatat tgccccagtt ctcaccgccg taggagtgga
    19441 actggacgtg ggtgtggtgc atcacctggt cgcggccgaa cttgctgttc ggcttgatac
    19501 cctcggagag cttgaacgtg tcgagcgtcg tcgtgtagtt gcccgggttc ccgaggttgt
    19561 tcgcatgaac gtgcatcgaa tgcgggagac cgaggtactc gtttgcctcg atgaggccct
    19621 tcacgatctg ggccggcgtg atgtcgaagt acggtaccgg gtcgtgaacg ttctcgcagt
    19681 tcaagcccca gccccaggct tcggtgccgc cggggttgac gaccttgacc gcgtaaccct
    19741 tcgtggcccg cagcagccag gcgatgtagg cggcggtgtt ctcgatctcg tggttcttca
    19801 ggtactcgag cacgaaccag ttgttcccga agaccgggta ggcgccctgg tcgaggatcg
    19861 gcgtatcccg catctcctcg tgggtgtgcc gggcgtagag cggcggcatg gccgcttcca
    19921 tcaccgtggt gtagcccatg cgggcgtagt tgtagccggt ccggatcgtc gtcggcaccg
    19981 agaacccgcc ctgcatccgc tcgatgccgc gtccgggctt ataggtgaag agcttgtcct
    20041 ccggccggaa gtttcggccg atgttcacct tcggcccggc gacgtgggcg tggatatcga
    20101 cgccgccggc catgacggtc ttacccgccg catcgatgac ctctggggcg cgaagcgcat
    20161 ttgtctcgac gatcttgcca tccttgatgg cgacgtcggc tttatcgccc ttgatcccga
    20221 gcaccgggtc gaagacgaaa ccgttcttga tgagatattc tgccatcgct tataccccct
    20281 tgatctcctt gacgcgggca aggacgcggt cgaggaactc ttcgtcggtc agcataccct
    20341 ctgggggctc aacgactttt ctcgtctcaa tcggcacgtt gtccatacgg taggcgcatc
    20401 cgcccacctc gacgcccacg aaggcgaccg ggacgtggac cttgcagacc tccgtggtcg
    20461 gtgtctcgtg cggttcgacg gcgaccgagg gcaggttgta gatctccttc accgagctga
    20521 aggggaagtg tgcgccggga tcgcttccga gcacgaagac ggcgtccacc tcgccccggc
    20581 ggagcaggtc gttcgatgtc gtctcgccgg ggttatagcg ggcaaacccg cgagagaggt
    20641 ccactgcgta ggggaacccg aacagccagc cccagacctg accggagccg gtgacgttgt
    20701 agtgcccgcg catcggcatg atggccgctt tcgtgtactc gttcaagtca cgggtgaccg
    20761 cgatggccgc atcgatgttg tggttctttc cgagtgactg ggtcacgccc ataccgaaga
    20821 agatgatcgc gaaccggccg ctcttgagca tatcggcggc ctcgtaaatc ttctccttcg
    20881 ggatgccggc gacgacatcc gggagctttt cgcccttgaa cgccacgcgg aacgcatcga
    20941 ggagttcgta gtcgcgcccc tgctcgatct ggaggtggac gtccgctatc cgggcggtgt
    21001 cggtgggccg cgggtcgacg acgatcatct tccggcccgt gtggccctta ccggtgaaga
    21061 acccacgggg gaagatcgag taccgggaca tatgccgcgg gtgggcgtgt gccgggttgc
    21121 acccccagaa gaggatccgg tcggcacggt tcttgacctc accgagcgtg cagctcggta
    21181 tgccgatatc ctgaacagcg ataagggatg ttccgtggca gacggttgcc gtgttatcca
    21241 tgaccgcccc gaccatctcg ccgatctcgg acccgacggc ctgggcctcg cagttcgtgg
    21301 agctccagcc gtacatcagg ggcttctttg catcggcaag catccgcgct gtgtactcga
    21361 ttgcctcgtc gtaggagatc tccttccagg aaccatcgtc ctgccgcatc cgggggcggg
    21421 tgagacgatc cgctgcctgg gagtggagaa acttctcggc gccgatggcg caggcgttgt
    21481 agacctcgaa gagtttcttc ccgtcatcgc tgacgacgac ctcgaggtcg tcgcagagtg
    21541 tcccgcagaa cgggcagacc acatcggtta ctgtctttgt catgccagct cacccctgca
    21601 tcctttgcgc accagttcaa gcgaatcaag gacaggttcg tttttggcga cctccaccgt
    21661 gacgggtgta cccttaaaag ttggcatccc cgtcgagtag gtgttcggat tgatcaccct
    21721 gtttgcccat gggcccatcg gtatatacgc cacaccggga tggggcccct gggttgcctc
    21781 gactgccttg acaacgacgc tgccatactg actggtgaca cgtacggtcg tgttgcgaaa
    21841 agctccgagc ttcttgaagt ccgccggatc gagctcgatg atcccgcagg cggcggtgta
    21901 ggcgggtttt tctttccccg cctccatcga caccccctgc tggatggtgc gacccgtgat
    21961 caaatttact ctgattgcca ttattattca tcccctgtta tattgtattt atctggcaag
    22021 ctctttgagc gggcttgggc caagctcggt gatggtcctg acgacgtctt ccatgacgtg
    22081 cccccacttt gcaccctcag atgcggagac gaaggtcatc ctgagacgct tgtcgtcaag
    22141 accgatgttc ttgaggacac tcttcaagag gaacatcctc tttgcagcct tgtagttgcc
    22201 ctcaaggtag tggcagtcgc caaagtggca cccggagacc agcacgccat ctgctccatc
    22261 ctggaatgcc ttcaggatga agagggggtc gacacggccc gtgcacatca cgcggacggc
    22321 acggatgtcc ggcgggtact ggatacgggc accgccggcg aggtctgcac cggcatagga
    22381 gcaccagttg cagatgatgg cgatgatctt gggtttccag ttctcgtctg ccatttactg
    22441 ttcacctcca aggaggaatg catcgatctg tgcaacgatc tgcggtgttg caaagtgctg
    22501 catccagata gcaccgccgg gacagaatcc gccgcaggtg ccgcagccct tgcacttggc
    22561 ctcggtgaca tgcatgaccg tgcggccgtc cttctcgatg agcgagaggg cctggtaggg
    22621 gcagaggttg acacacagtc cgcatccggc gcacttgtcc tccatgcaca cggcgaagta
    22681 aggctcgagc tccacctcgc cgcggtggat cgggatggat gccgcggatg ccgcaccctc
    22741 tgcctgggcg accgtatcgg ggatatcctt gggtccctgg cagacgccgg cgaggtagac
    22801 accggcagtg gtggtgccgc aggggttcag cttcgggtgg gcctctaaca gccagccgtc
    22861 ctgcgagcag gagacaccga agagtctccg cgtcttctcg gtatcggcgg agggctggac
    22921 ggcggccgcg agcacgacca ggtcgacttc catatcgatg ggccggccaa gcagcgtatc
    22981 ctcagcgttg acgtggaggt tcttcgtaac cgggtcctcg aggatgtttg caaccctgcc
    23041 gcggatgaac ttcgcaccct cgtcctggat gcggtagtag aactcctcgt acatcttgcc
    23101 gaaggaccgg atgtccatgt agaagatgta ggggatcgcg ccggggatct tctcgatgat
    23161 ctggtgggcg tgcttgagcg agtacatgca gcagaaccgc gagcagtacg gcttgccgac
    23221 gccggtattg tcacgggatc ctgcacagag gacgaatgcg atcctctttg gggtctcgcc
    23281 gtcgcttggg cggatgaggt ggccgccggt cggtccggac gcgcagatca accgctcgaa
    23341 ctcaaggctc gtgatgacgt tgtcgtagtg cttgtaaccc cactcgaact tcttctcgat
    23401 cgggaagata tcgtatccca gggcgaggac gacggtgccg accttgacat tcatgagttc
    23461 gtcctttgca tcgagatcga tggccttcct ctcctcgccg catgcctcga cgcagaggcc
    23521 gcacttgacg caggcgtcga agtcgacggt gtagatcagg ggcgagacct gcgggtggta
    23581 gatgtagatc gcctttctcg gcgccatgcc gagctcgaac gggttcggct tgatgaccgg
    23641 acagacgtcg gtacagtcgc cgcatccggt acatccgccg ccgatgatgc cgcgggctgt
    23701 ggcctcatcc ggagtgagaa cgccgcgcgc cttcttccgg agggtcacgt cgaagttgcc
    23761 gatgtagccc tcgaccgcct cgacctcggt gtaggtgagg agctcgatgc cttcgtggcg
    23821 gtagacatcc accatcttcg gggtaaggat acactgcgag cagtccagcg tcgggaacgt
    23881 cttgtcgagc tgggacatct tgccgccgat ggtgggttcc ttctccacga ggtaggtctt
    23941 gatgccggcg ctcgcaaggt cgagcgcggc ctgcatgccg ccgacaccgg cgccgacgac
    24001 catcgccgtc ttctccacgg ggacgctctt cggtatgaga tcctcaagga gggatgcctt
    24061 tgctacggcg atcctgactg catcttttgc ctttgcggtg gcctcttcgg gctggtgcat
    24121 gtgcacccac gagttctgct cgcggatgtt ggccatctcg aaccggaagg ggtttaagcc
    24181 gccctccttg gtcgcgttac ggaacgtggg ctcgtgcagg cggggagaac atgctgcaac
    24241 gacgatcccg gtcagtttct gctccttgat ggcctctgcg atcttgttct ggccgggggt
    24301 cgagcacatg tactggtagt catcggcgat gatgacgttc gggagcgttt ttgcgtactc
    24361 ctgtaccgcc ttgatgttga tcgagcccgc gatgttggta ccacagtggc acacaaaaac
    24421 gccaattctt ggctcttcgt actttttgac ttctgccatg tatttgcacc tcacaggatc
    24481 ttcttcagga acggctcgac actgacggcg tggaggtcga gtgcaagctc atccgggctc
    24541 atgccctgcg ccagtcccag gagttcgttg tagtgcagta tcggaagccc ccaggtgatg
    24601 ccgaacttct cctcgatctc gatctgccct ctgtcgtact ggagctggca gaacggacag
    24661 acttcagtca gcgcatcagc accgacctcc tgcagattga tcatcttctc gttcgtgata
    24721 tcgagtgcat gcgcgatatc gtatccgcgg acaccgccgc cggcaccaca gcactgcatc
    24781 ttgttgcggt actgtaccgg ttcggcgccg agagcggcga cgagctcttc tatccacatc
    24841 gggttctcgg tgtcgccgaa gtgccgctct ttggcgggct tcatcaggtg gcaaccgtag
    24901 tgaacggcga tcttcgcgcc ggtcaggggg cgcgtgacgc tatcggcgag cttcttgata
    24961 cccacgatct tcgggtcgta gtagagctcg gcgaggtgcc agacgtcgac ggttcccttg
    25021 aactccatat cgatctcttt gagcacctcg ttgacgccat cgcggagttc gtcgttgtgc
    25081 ttgagcttgt ggttgacttc ccagatggac ttgtagcagc cgttgcagat cagggtgatg
    25141 tccgtcttca tctgctcggc aagcacgacg tttcgcgcag ccattgcgta ccagacgttt
    25201 aagtcgatcg aaccgaacgc gcccggcgcc gggcagcagc ttgcgccctt caggggaagg
    25261 aggtcgatgc cgacattcgc gctggtcttg atggccgccg cctcgatccc ggggtaccgg
    25321 ttcggtgcga tgcatccgag gaagaatgcg tactgatgtc tgttgctcat ggtttcactc
    25381 accctccgcc agtatcctgt ctgcattctc tttgaggcga tagtgatcca taatcttcct
    25441 gataccgggc agatactccg gatacttgtg cgtcgtctcg ggttccgcct caaggccgag
    25501 cttctctcgt gcaacccggt ttgcagcgtt gttcgggacg ccgtggcccg actggtagat
    25561 cagctgggct gtcttgagga agttgcgcgg cacgatatcc cgcttgaacg caaggttcct
    25621 catcgccatg atcgcgtcgg tcggcgcgag gtcacggggg caccggtcgg tgcagctgta
    25681 acaggtggtg cagagccaga ggtcggggtc ggtgagggct tcctcctcga ggccgaggat
    25741 ggctttcctc atgaacagcc ggatacggta cgaactccgg ggtgccgagg ggcacgagcc
    25801 ggtgcaggtg ccgcactggt agcacatgtg ggcggccgtc cgcccgatct tctccacatc
    25861 cttgatgaac tcggggttgc tgtcgggaat gtagtacttc ctgtcccgga gtctctcagc
    25921 gagtttctgg tccttatagt ctttcttcac tgccattgac tatccctcac gcctcttcca
    25981 cttttcccag gactttgatc tcgacttctc caggagtggt ctctttcacc ttcgatgtcc
    26041 tcgtggtgag cagtttttcc ttgatgtccc tgaagagttc cgaatccttg tcctgcatcc
    26101 ggatagcggt tcgccgcagg atgatagcat cttcgctcgg gcaggcgttg acacaggcgc
    26161 cgcagaagat gcagaaatcc ttgttgatgg cgatgttcgg ttcgatcctg cctttcatct
    26221 cctttgccgg gagcggtgtg ggcaggtaga gtgcattgca ggggcagacc tcgacacagg
    26281 tcgaacaacc gccggggcac ttctcggggt tgatctcgat ctctccctcg aagatcttct
    26341 cgacggtgat tgcctcggtg gggcaggtct ggacgcacca ggtgcaggta caacagtcgt
    26401 cctgctggat gtcgaccttg ccgggcagtc tgtcgctcac gacctcgcgc tcaagggtga
    26461 ttgcgtcctc ggggcaggcc tcgacacaga tcttacatcc gtcgcaggtg ctctcctccc
    26521 acaggacatc cccttcgatc ttgccggtct cgggggttct gggtttgcgg acgacggtga
    26581 tgctcgggca gagttcgccg cagatgccgc agacgctgca cttctccatg tcgactttaa
    26641 acgtggtctt cgtctgcaga gcggtctcac ggggtctgcc ggcctcttcg ccgccctcga
    26701 atgcggggac gtcgcggttg atggcgtctc tcgggcagac ctcctcgcag atggtacacc
    26761 ggtcgcactt ctcttcgtcg atcacggtga ccatatcata ctgggggaat ccttctttct
    26821 caagtatcgg gagccgttct tctccgtcga tcttgagcgt catcgcattg aaggggcaca
    26881 tgatgacgca gacgccacag tatgaacact tttcagggtt gacgtcgaca ggttcggcgt
    26941 aatcgatcgc cccgcggcgt gtcgcaccga ctggtccgag cacgattgcc tcttcggggc
    27001 aggcatcgac acagatgccg cagcctgtgc aggtctccgc gttcaggata aggttgttga
    27061 ccgctttgag gagcttctgc tccatgatga cgttctgtcc ttcccgcgtt ttggaatact
    27121 ttggaaacag tgccatattt tagtatcact cctaggcttc cactcttcgg gcctttgccc
    27181 ggctctccca cggtcctgca agtttgataa cttcgtaggg gcatgcctcg acgcatacgc
    27241 cgcaaccggc gcagagctcg gagttaaagt caagaataat ggctttgccg tccttgacct
    27301 tgtagatctt ctccagggtt accggttctt cggtatggag ctcgagagcg ttgaccggac
    27361 atgccaccac gcagttgttg cagcctgtac atcgttccat attgatgtgc aatgcaaatg
    27421 ccatggttat cacgcaccag atctgatcga ttaaaaatcg atataaaaag aattgtcaaa
    27481 gaatatacat aaactttctg aaattcggca gtcttatatt ccagaactat gattattcgt
    27541 caaattttat aaattaaatc ggttgttatg ttaacccgat gtcacggata tcggcgcaaa
    27601 acgcatctcg gggcagaatg tcaaagcgaa aaccttaaat tttaagtcgg gccttctggt
    27661 gaaaattggt ggccaatccg ggccggcagc gtgaagaacc ggccggatct ccccgggacc
    27721 cggcgataac ccccaccttc ctccccggaa gagcccgccg cccgttgcgc ggtttgggcg
    27781 acacctgttt acgtaatcgt tggttctcca ccctttgcgc cgggcacgtg cgcccacccg
    27841 gaatgcccgt cgcgaccctg cgcccacggt cctccatagc atcccgttca ggcccggtcg
    27901 ggcgggatcg tgggaccggg aagggcttaa cccggcctcc gacgcggatc cgggagatat
    27961 ggatgttttg caccccaata gcgcaatcag gctgaatttc tgcgttctgt cgtccggcag
    28021 ccggttcttc gccggtttac cctgccgtgg cggtggctgt cggcgggccg gcccggtctc
    28081 cgggggggcc gcgcggttga ggctgccgat ctccggcgcc gatccggtgt gcacgcccgg
    28141 gagaaaaaga aaccttattc tgtccgcatc gtgacattgt agcaaacgag gtgtattctg
    28201 tccatggaat tgaatggcgt tactatcgac gatacctatg cagaggcgtt cccgacctgg
    28261 gtggcgcggc ccatcatcac cgctgtcacc gaggaatggg catacagggc ggcagtggag
    28321 gccgtcggtt ttgccacctc cacgataggt tgtccggctg aggcgggcat cgactgcttc
    28381 gtccctgccg acgagactcc cgacgggcgg cccggctacg caatcctgat ctgtgcgagc
    28441 aagaagaagc tcaaggagca ggtcgtggag aggcttgccg agtgcgtcct caccgcaccg
    28501 acgacggcgg tcttcgacgg ccttgccgac gtcgttgccg aggtccagga gaaactcccg
    28561 gtcaagctcc acttcttcgg cgacggctac gagcagaagc gcgaggtcgg cggcaggacg
    28621 gtctgggcca tcccgatcat ggagggcgag tatatcggcg aagaagagtt cggtgccgtc
    28681 aagggtgtcg cgggcggtaa cttcttcatc atgggcgaga atcagatggc cgcgctcatg
    28741 gccgcgcagg cggcggtcga cgccatcagc ggcgtctgcg gcgtcatcac gcccttcccc
    28801 ggcggcatcg tcgcgagcgg ctcaaaggtc gggagcaaga agtacaagtt catgggcgca
    28861 agcacgaacg aggtctactg cccgaccctc cgcgataagg tcgaggggag caaggtgccc
    28921 gaaggcgtca aggcggtcta cgagatcgtc atcgacggcg tcgaccagga ttcggtccgg
    28981 accgcgatgg ccgagggcat ccgggcggca tcgaaggtgc cgggcgtgaa gttcatcagc
    29041 gccgggaact tcggcggcag cctcggaccg ttcaggttcg agctcaagga tgtccttgcg
    29101 gattaccttt aaaccttttt ttgttgccta tccacggttc ggggccctgg atcgcacgga
    29161 taagcctccg gccccggccc gggcgggtgc ggcgcaccat ggtgcggaca cggtcgtgcc
    29221 cgggtatgcc gggcccatat gattgatggc ggcctgctcc ccatgcccgc cggctcgccc
    29281 ccgatgccgg gcatacggtg cgcatgaccc tcgtgcgccc cctcgcacgc cccggccctc
    29341 gcgatcgatc cccttaaata tcgttatcaa cgaaattctt gttaacgatt atttcggtga
    29401 catcatgatc acgctcacac ccgatgacgt cagggcccgg ttcgggcctc tcttctcgat
    29461 gaagtacctt gccatggtcg accagaacgc cggccttgcg gagatccgcg agcattgccg
    29521 ggcgcgggga acgatcgagt gggatgcggc gaaccgcatc cgggcgggcg gggccatccg
    29581 gtcctgtcac gtcgagggca cgacgatgac gatgcttgcg cgcctggggc tatctccggc
    29641 gaagttcggg gcggccggaa gggagatcgg cggacaggcg ctcgagggag tcgaggtcga
    29701 cggggacgag gtggtgacga cctggtcggg tatcgccggg gccggtgtcg gcgtcgccgc
    29761 ctgcctgacc caggcgccgg gggtgatccg ggccgagtat ccctccgagg acgacctccg
    29821 tatcggcggc gcccgggtat gccgggtccg gatcgtatcg ccgctctacg agaaggtgac
    29881 gatcgggatc gacgataccg acacccggga ggagggcgcc acgtgggtgc tcgcgctcaa
    29941 gtgcgccgag gcctgcacga tccccggggt ggagtacctg gacatgcgcc tcgtgcagtt
    30001 gaaccccgcc gtcccgaaga agaccaccaa ctgcgtcggt tccgccctga acttcgccgt
    30061 ccggccgggg agagtggacg ccctcctcga gtacgtccgc gacttcatcg agtccgaagc
    30121 ggtcagcaaa gacaccggca tcgccgtata caggggaatt gcgtttgcag aggagtcttc
    30181 ctacgcacga agggtcaaga ccgaactcct gacgcttgaa gaggcggagg cggaggccgc
    30241 ccggatgggt gtccggttca tcgactcgaa ccggcggaag ggccgtatag gggcactcgg
    30301 cgccgtcctc tggggtaaca agggcgtcga agcggcaggg ctctatggag agactttctg
    30361 atccctacac gatacgctac ccgcagatcg tggcggtggc cgacgagagc ggcgggcacg
    30421 tcgaactcat cgagttcttc gactgcaccg gcggcgcgat gtgggtgaag cgccactacg
    30481 cgcagagccc gctcgtccgc tccgtccgca ccgtgggcgc gaccaaccgt tacatcctct
    30541 cggccggcag cgccgacctc gctctcgaag gttcggtctt cccggcgggg atcgccgctg
    30601 tcgccgtcca gggcgacgag atcgccgtca cctaccgggg cctcggcggc ggcggcgtgg
    30661 gggcatccat ctgccgcgca tcggcatccg gcgtcgttcg ccacaaaagc gatcccgccg
    30721 gcggcgggcg tcttgcgggc tcgacgatct ggctcccccg ccgcgagcgc gtggtcatcg
    30781 ggatcgacga caccgatacg ccggaggaag gcgccacctg gacgctcgcg cacaacatcg
    30841 cgcgggccgt cgaggacgac cgttcccgct acctctccca caccatcgtc cagctctatc
    30901 ccgtccccta ccggaccaag aactgcgtcg ccatcgcctg cgagttcgcg acctccgacc
    30961 ccggtgggct tgcccggcac taccgcgacc tccttacgga gtacaccctc tcggaagaga
    31021 ccggtatggc ggtctggcgc gggtttgaca ccgcaccgct cgaagagttc ggcggaaagg
    31081 tgaagcgggg cgaggtctcg gcagacgatc tcgccgccat aaccgatgga aggctctccg
    31141 ttctcatgga cggccggggg gcgatcgggg cggtggcggc aataccgttc ttcacgcgat
    31201 acgaggaggc gctggcgcta tggaatgggt ccggctgaaa gcccgcctgc tcgaggcggg
    31261 ctctgtccgc ctctccggcg aacccgcgga tcagtacatc tcccgctccg ccgccggccc
    31321 ctctgcgggg gcgcccggtt cgctcttctt ttctctcggc gataggcggg tccgggtcgg
    31381 tatcgatgag acggccccca tcgaggtcat ccaccgggga gagggcgttg ccgacctcct
    31441 catcggcggc gacatggtcc gcggcaggct cgaggccgcc gcgctccact gtccgcgcca
    31501 ggcctacgtc accgtcagcg ggacgtgcat ctttcactgc cgctactgca cggtgccgca
    31561 cctccccggc cggcggaagg ggatcgccga agtcgtgggg atggtcgaga gcgtggccga
    31621 ccggatcgac gccatcgcga tcacgagcgg cgtcgcctcg tcgatcgagg aggaggaggc
    31681 ctacgtcctc gacgtcgtcg cggcgctcgc gcccttcggt ctccccatcg gcgtctcgat
    31741 ctacccgggc ccggagaccc cggcccggct ccgtgccctc ggcgtcgtcg aggtgaagtt
    31801 caacctcgaa gcggcgacgg agacgctctt tcgcgatatg tgccccggcc tttcgtggga
    31861 ggcggtccac gaggcgcttt tccggtccgt ggcgctcttc gggaggggca gggtcttttc
    31921 aaacgtcatc gtcggcctcg gggagaccga cggggacctg gagcgggtga tgaccgatct
    31981 cgccgcggcc ggggtcatac cggtgcttcg cccgctcacc ccggcggcgt ccctcgccgg
    32041 ccggccccgg ccccccgccg accggctgct ccggctctct gccgcccacg agcggatcct
    32101 cagggaggcg gggctcgacc cccgccgggc gctgacgatg tgcccggcat gcacggggtg
    32161 cgacctcgtc cccgggaggg acacatgaag ggcgaggagg cgctcgcccg cgccatcctc
    32221 cggtccgccg accggtgcta caccgtcccc ggctacccgg tatcggatct tgccgggcga
    32281 gtcggcgccg cgatcaccgc aaacgagaag gtggcggtcg agtacgccct cggcgactcg
    32341 ctctccgggc ggagggccgc cgtgatcgtc aagcacgtcg gcctcaacgc ctgcgcggac
    32401 ccgctcgtgc atgcaacggc ccagggtctc cgggccggcg tcgttatcgt cgtcggcgac
    32461 gatctgcgcg cggccgcatc agacgtcgtg caggactccc gctactacgg cgaggtcgcc
    32521 cgggtgccgg tgctggaacc cgacggcgag acgatcggcc ccgcggtcga agcggcgttc
    32581 gaagcctcgg agaccttctc ccgcatcgcc atcgtcaggg tcacgcccgg cctcctggat
    32641 gcggatgtcc cggagacccc cgcaccccgg agcggccggg aggggagcct cgcagacccg
    32701 gggctcacga tggccgggcg ggcgcaggcg gccgaccgcc ggacggcggc catggttgcc
    32761 tggtcgcgga cctcgcccct caaccgttac gagggggatg tgttccacgc cgtcaccgtc
    32821 tacccgccgc cggcggatcc ggacgtgctc gcccggatcc tcgaggccgg ccgccccttc
    32881 ctccgggagc accggctgct cgagcctccc gccactaccg gagagccgga acggttctcc
    32941 gcgcggggct actcccggac gttctgccgg gactgcccgt tccaccctgc ccttgccatc
    33001 ctctccgaac gcaggatgcg gaccacctgc gacgccggat gcgctatcct cgcgatgaac
    33061 ccgccctacc gggtagggct tgccacctac ggcctcgggt cgtcggttgc cgtggcggcg
    33121 accggcccgg gcatcgcgct cgccggcgac tacgctctcc tgcactcagg gctcgccgcc
    33181 ctcatcgacg tctacgaacg agggctcccg ctcctctgca tcgtacttgc gaacaaccgg
    33241 atggggatga ccggcggtca ccccgtccct gatatccttc gctacatcgc ctgggcggac
    33301 ccggtcgtct gcgctgcaga cgacacgggc gctcttcgcc gggccctcac cctcccggag
    33361 gagggccccc ggacggtggt gatcaaggga acctgccctg aaggtgcaca ccatgaaacc
    33421 ctggaatatc gagatctgtg atgtaacgct gcgagacggg gagcagactc ccggcgtctc
    33481 gtttacccat gatgagaaga tgacgatcgc acgagcgctc gatgagatcg gcgtggaggt
    33541 gatcgaggcc gggttccccg tggtgtcgtc ggcggagaaa gagtgcgtcg ccgcgatcgc
    33601 gcgaagcggc ctctccgccc gggtctgctg cctagcacgg gcgctcaggc ccgacgtcga
    33661 ggcggccctc gactgcgatg tggatatggt cagcatcttc atagcgacat ccgacctcca
    33721 catccggcac aagtaccgaa agccccgcgc cgaggtgctc gacgatgctc tcgacatggt
    33781 ggagttcgcc gccgatcacg gcgtgcaggt gcggttcgcg gccgaggatg cctcccggac
    33841 cgatcctgcg ttcctcatcg agatgtactc ccggggcgca gaatgcggcg ctgatctcgt
    33901 cagtttcgcc gatacggtag gatgcctcgc accgcttgag atccacgcgg tcatatcccg
    33961 gatccttggc gacgtccatc acccgctctg tatccactgc cacaacgatc tcgggtttgc
    34021 ggccgcaaac accatcacgg cggcggctgc cggggcgttt cagctccaca ccaccgtcaa
    34081 cggcatcggg gagcgtgcgg ggaacgccgc gcttgaacag gtgctggtcg ccctgcggat
    34141 gaaaggcggc gtcgaccgct acgatctcac ccgcctgcag gagatctcgc ggctggtcgc
    34201 ccggtgttcg ggcgtggtcc cggagcgcac ccggccgatc gtcggcgaga acgcctttgc
    34261 ccacgagagc gggatccaca tagccgccat ccttgaggat ccggcgacct acgagtccat
    34321 cctccccgaa ctggtgggcg gcgagcgccg gttcgtcctc gggaagcata cggggaggaa
    34381 agcgcttgag catatcgcgc aggcgtatgg gttcgacctc tccgacgggc aggtgcggtg
    34441 ggtgctcgag cagatcaaac agaggagcga ggggaaatgc agcgtcaccc ccgaggtgct
    34501 ctgcgggttc ctccggcagg cgaaggcgga ggtcctctcg tgagcacgct ctcggagcgg
    34561 atcctcggtg cccctgccgg cgcatacgtg gaccgcgagg tcgatatcgc ctttgcccac
    34621 gacgggaccg gcgtcctcac ccgggaggcg ctccgggaga tgggcgtgga gcagctccct
    34681 caccccgagc gcctgcgcct cgtcttcgac cacatcgtgc cggccaacac cggcacgacg
    34741 gcgacgctcc aggcggagct ccgcgggtat gcccgggcct cagggataga cctctcggat
    34801 gccggcggag ggatctgcca ccaggtgatg agcgaagggg tcgtccggcc cggcatggtc
    34861 gtcgtgggcg ccgactctca cacctgcacc ctcggcgctc tcggggcgtt tgccaccggc
    34921 gtgggagcga ccgatatggc ggcgatctgg gcgtcggggg ctacgtggtt tcgggtgccg
    34981 gagacgatcg cggtcaacct cacgggaagg ctcggtggcc ccgcagagcc aaaagacgtc
    35041 gctcttgcct gtgtcgccag actcggcatg gagggggcga cctaccgggc gctggagttc
    35101 gtgggtgacg gcgcgtcggg gatatccatg gacgggcggt tgaccctctg caacatggcg
    35161 gtcgagacgg gagcaaagac cggcatcttc tacgccgatg cggtcaccgt ccgccatctt
    35221 gccgggcacg gcgttgcggc gtcgccccag gctgtggagg aaccgcgcta cgagcgggag
    35281 atcgccatcg acctctccgg catcgtgcct cttgtcgcgg tcccgcaccg ggtggacacc
    35341 gtccgggagg ccgaagatgt cgcgggcacg ccgatcgacc aggtcttcgt cgggacctgc
    35401 acgaacggcc gctacgaaga ccttgcccgg ctagcccgga tcgtccgggg gaggaaggtg
    35461 gcggtccgca ccctggtcgt ccccgcctcc cgcgcggtgc ttgcccgggc gatcgccacc
    35521 ggcgtccttg ccgatatcgt ggatgcggga tgcatggtgg ccccgcccgg gtgcgggccg
    35581 tgtcttgggg cgcacgaggg cgtgatgggg gagggcgagg tctgcctctc gaccgccaac
    35641 cggaacttca agaaccggat gggcgtgggc gccgagatct acctctcatc ggtcgccacc
    35701 gccgcggtga gtgcgctcgc gggcgagatc gccgtgccgg aggtggcatg atgcagggaa
    35761 ccgggccggc cgtctgcctt ggcaacgacg tcgacaccga cctcatcatc gcgggccgct
    35821 acctccggac gaaggaccgc tcggtctggg cggagcacgt ccttgaggac ctggacccgg
    35881 cgctcgcccc ccgtattcgc ggcgcgatca tcgtcgccgg gaggaacatg gggtgcgggt
    35941 cgtcgcggga gcaggcggtt gtcgccctcc gggaggccgg tgtcgtcggg atcgtcgcgg
    36001 agtcgtttgc ccgcatattc ttccggaacg ccgtcaacgt gggtctcccg gtgatcgagg
    36061 cctccgttgc ctgcaccgat ggcgcccacg tcgctttcga cctcgatagc gggtgggtgg
    36121 aggtggacgg ggtgcgccgc cccgcccgcc cgctctcgcc gaggatggtc accattctcc
    36181 gggccggagg actggtcccc tactggaggt cgtgccggtg atatttcccc cgcattgcaa
    36241 atacgtcggg gccgtgaccg gcacaccgtg cggggagcgg gtctacttcc tctcgcgcta
    36301 cctggtccgc gagaccgaac cggacggcat ggaggtgctc gaggtcgagg ccgatccggc
    36361 cggaaccggt ttgatgagga gcatcctctc cacccgggtg ctcgccgccg gcgacgaggt
    36421 ctgccgttac ccggacccgg tgaacgtcca ggaccgcaca ttcctggtga gggcggccct
    36481 ccggtccggg caccggtgca cggtcttctc cggccacggc tgtcagacca cgttcgtgct
    36541 cgaccccgac ctctcggggt tccttcgcct tcacgtctac gacatcaccc cgccccgccc
    36601 ccacctctcc gccacgctcg gcgaccttga gaggaccggt ctcttcggcg acctcgaggt
    36661 catattcgag caccacgtca gggatatcag ggagatcgag gccgacgtct atccctgccg
    36721 cgcggccggt ttcccccgca ccctcgatgc ggacccggtc cgcccgggcg accgggtcgc
    36781 gggatgcctg acggcgaaag acctggtgcg ggagtgctac ggggaggaga tcggcgtgga
    36841 gagcatctgc ccgcttggag cggtgacggc agaacctttc gtcgcccggt gctgccggag
    36901 cgagcggggc ggtctcgggc gctggaacgg caagttcggc gcggtggtcc actggggagc
    36961 atcgtcgtgg gagattgcgg atgcggtcag gcaggtcgcg gccgcgtgga gggagcggaa
    37021 tggtgaaggt agcggtcgtt gagggcgacg ggatcgggcg cgaggtcgtc ccggccgctc
    37081 gcgatattct tgcggccgtc cgccccgaca tcgagttctt cgacgtcgat ctgggttacg
    37141 ggctctggga gcggaccggg tgcgcctgcg gggaggagac ggttgccgac ctcggatcgg
    37201 cggatgccat cctctttggg gccgtcacga cgccgccgga ccccggttac cggagcgtca
    37261 tgctccagat ccgccacgcc ctcgacctct acgcaaacgt ccgccccatc cgtggcgaca
    37321 ctgtcgacgt cgtcatcgtc cgggagaaca ccgaggggct ctactccggc atcgagtgga
    37381 ccgagcccga ccgctcctgc accgtccggg tcgtctcccg ccgggggagc gagcggatcg
    37441 cccgctacgc ctgcaccctc gcgaagtccc gccggcacca cctcaccgtg gggaacaagg
    37501 caaacgtctt aaagtcggac tgcctcttcg tcgaggtctg cacggcggtg gccgcccatc
    37561 tgggagtccc ctgcacggcc cgctacatcg atgcgctctc gcttgacctc ctcatgcacc
    37621 ccgaccgcta cgacgtgatc gtgacgacca atatgttcgg ggacatcctc tccgacgccg
    37681 cggcgtacct cgtggggggg ctcggcatgc tcccgagcgc gaacatagga gaacgtgccg
    37741 ccctctttga gcccgtgcac gggagcgctc ccgacatagc ggggaggaac gtcgcaaacc
    37801 ccatcgcggc gatacggagc ggtgcgatgc tgcttcgcca cgtcggcgac cctgcatccg
    37861 ccgcagccgt cgaggaggcg gtcgtgcggg tgctccgtgc gggcatccgg acccgcgaca
    37921 tcggcgggac cgccggcacc cgggagttcg gggcggcggt gctccgcgag gtcgggcggg
    37981 ggaaggccta aacgccctcc cgccaaggtg taggctatgg tgctggtagg atgccacgtc
    38041 tccatcgcgg gctcgatcga ccttgcggtc gggcgggccc tcgattcggg ctgcgatacg
    38101 ttccagatct tttcgagaaa cccccggggc tggaaggcaa aagacatcga ccctgacgtg
    38161 gccggtgctt tccggagagc gctcgacgca tccggcatcg gtccggccgt cgaccacatg
    38221 ccttacctcc cgaacctggc ctcgcccgac gatgcgatct acgaaaaatc ggtcgcggcg
    38281 ctcaccgacg aactccggcg gtgcgaggtg ctcggtatac cctacctcgt gacgcatctt
    38341 ggccatcacc gcggcgccgg gaccgggacc gggcaggaac gggtcgtcgc cgccatcaac
    38401 cgggcgcttg aagaggccgg cgcacaaggc gtgatgctcc tcctcgagaa caccgccggc
    38461 gagaagaaca gcgtcgggtc gaccgtcgcg gacctctgcc ggatctttga gggtatcgac
    38521 gggaaggaga ggatcgggat atgtttcgat acctgccacg cgtttgccgc aggctacgac
    38581 ctccgaaccg gggaaggggt cgatgcggtg ctcgaggaga tcgacgacct gatcggcccg
    38641 gggcacctcc ggatcatcca cctcaacgac tgtaagggcg acctcgggag cgggcttgac
    38701 cggcacgagc atatcgggct tgggtccatc ggggaggagg ggttccggca tatcctcagc
    38761 cgtccggcca taaggggatg tccgctcatc tgcgagacgc cggtggatgg acggcgggga
    38821 gacgatggaa atatcgcgaa ggtccgcgaa ctcgccgggg catgaatttc tctgcgatac
    38881 cctatcctca taatattcgc taatttttat aataattacg aatacttctc tcggctcccc
    38941 ggggccgcaa gggatatata tttatacccc catcatgccg ttcacgaacc ctttgcccgg
    39001 caggacccgg tgactctccg gcatcctccc ggacctcagt ttgcacgcgg gcacggccgg
    39061 gaagcttctc tctcctgcct gcaggcacca tcgtgtgaga cgaaaggggg cgttctcatc
    39121 atggtgatcg aggaatggct gaatacagag tgttaatgat agtctgcacc gtcatcgccg
    39181 ccggactgct ggcctcgccc gttctggcgg tggaggcagc gccgggggag acggtgctgt
    39241 tctgcaccgc cggcggatcc caggagcacc cggatatcga cggcgatatg gtcgtctggg
    39301 aggacggcag ggacgatcgg tctatctact acgctgccgc tcccggcagc ggtggccgga
    39361 aggttgccgg cggctacgcc gaagagcggt atccgtccgt gtcgggcgat ttcatcgcct
    39421 ggcaggaccg gcggcacggg aactgggaga tctacctctt ctcgccatac gacggcgaaa
    39481 ggcggatcac gaacagcacg accgaccaga cgatgcccat cgtgcacggt aaccatattg
    39541 cctggtacga tacgcgaaag gggtataccg atatctgcct ctacgacatc gcgaccggca
    39601 aggagacgta ccttggctgc tcgccgagaa ttgagtggaa actcgcgctc tcggagcgtt
    39661 acgtcgtctg ggaggagatc gccgggggca tccaggcata cgatatccag accgaggaga
    39721 aattggggat agcggcgagc ggtgcgcggc aggcataccc tgcgatatcg ggggaccgga
    39781 ttgtctggga agattaccgg aacgggctgc ccgatatcta catgtacgat ctcaatgagg
    39841 gccgggaata ccagatcacc gacgaccccg ccgagcaggt ctctccggcg atcgatggag
    39901 agatcatcgc ctgggaggac aagcggagcg ggatctggga tatctacatg tacgatctcc
    39961 gcgcctggac cgagatgtcg gtctgcaccg caaacggcga gcagctcttc ccctcggtct
    40021 ccggggagaa gatcgtctgg caggataagc ggagtgggag gtggcaggtc tacctcttcg
    40081 cctactccgg cggagcgccg ccggaggcgg agttctcggc ggatcccatc gccggcacgg
    40141 tcccgctcac cgtccggttc gccgaccggt ctaccggcag tccggatgag tgggagtggg
    40201 agttcggcga tggcggttcg tcgacggagc caaaccccgg gcatacctat gaggatcccg
    40261 gcgagtacac ggtctctctc cgggtgagca accgcttcgg gtccgacatc gtcacaaaga
    40321 ccgatctcat ccgcgcaaaa accctggaac ccccggccgc cgatttcacc gccgcaccgg
    40381 tgtcgggcag tctccctctc accgtgcggt ttgcggaccg atcgaccggc gcgccatcgg
    40441 cctggctctg ggactttgga gacggcgcga cgtcaaacga gcaagacccg gagcacacct
    40501 atgccgcagt cggcacctac gccgtcagcc tgaccgccgg gaacccggcg ggcagcacga
    40561 acatgacgaa acccgccttc atcaccgtcg tcagtcctcc aaaagcctcg ttctcggcag
    40621 atacccggag cgggagagcc ccgctcgccg tccggttctc ggacgcctcg accggcacta
    40681 taacggcctg gtcctgggat ttcggcgacg gcgggtcctc tcacgaccag aaccccaccc
    40741 acacctacac cgggccgggc atctacaccg tcgcgctcac ggccgcaaac gagtatgaca
    40801 gcgatacccg gacgatggaa ggctacatca ccgtcaccgc accgccggta gccgggttct
    40861 ccgcgaacgt gaccgccggt cgctcgccgc tcgcggtgaa gttctcggat gagtccggag
    40921 gaggtccgtt atcgtggtcc tggaccttcg gcgacggggc ctcgtcggca gagaaaagcc
    40981 cggttcatgt ctaccaggcg cccggggcct acacggtcac tctcaccgtg gcaaacggcg
    41041 caggggagaa cacgacggcc cggaccggct acataacggt tcgcgaccta ttgagcgctc
    41101 ggtttaccgc caacgtgacc gccggggccg ccccgctcgc ggtcctcttc accgacgcgt
    41161 cggtcggcga accccggtcg tggtcgtggg acttcggcga taaggggacg tcgaccgtgc
    41221 cggccccggt ccacgtattc gagcaacccg gtaactacac cgtcagcctg acgatcgccg
    41281 acggcgccga tcgttcgacg aaatccctgc agatccgcgc ctttggtccg ccgggtgcag
    41341 agttcagtgc cgctcccctg aacggcacgg cccccctcac ggttgcgttc accgacgctt
    41401 cttccgggga gccctccgcc tggctctggg acttcggcga tcaggggcga tcgatcgagc
    41461 gccatccctc gcacacctac accgccgccg ggaactatac cgtcaacctg accgtggaga
    41521 acgccgccgg gacggacacc gcgacgaaga ccgggtacgt atccgttcac cccgccccgg
    41581 taacgccggc accgacgagc ggcggcggtg gcggcggtgg aggaggtgga ggaggtggcg
    41641 gtggcggcgg tggcggagga tccacctgga cgaccccgga cccgggcacg ccgaccgcga
    41701 ctgcgacccc gactgcgacc ccctcccccg ggcctgccgg acccgacgtg ctccccctcg
    41761 gggagagcgg ccttgtgagc cggttcgtcc ggatcgcttc gcccgacggt gttgcaagcc
    41821 tcgccatcgc ggagggtgtc agggccgtcg atgccgttgg aggcccgctt cgcacggtga
    41881 ccctcaaact cctcgattca cccgacgtgc ccggggcgcc gggatcatat gtgttcagcg
    41941 ggtatgcctg cactgccggg ccggatgggg ccgccttctc accgccggcg accctggtat
    42001 tcaacctcac cgaggaggag tgggatgctg tctacagcag ccggaggggc ttcctggtgc
    42061 agtggtataa ccggtcggcg aacgcctggg aggaggttcc caccctcgtt caccctgaaa
    42121 ccaggagcgt cacggcagaa gtctcgcact tcagcctgta tgcgctcttc gtcggtctgc
    42181 ccggcgacgg cgcggtagag gttatcgcgc cggatacccc gcaggccggg gaggcgttcc
    42241 cctccatgca gatcatcccg ggattgattg cccttgccat cgtgggtgca ggggcatacc
    42301 tctactaccg gagaggggag ccctgaacag cgggtctccc cgcccgtaaa aatgcgcgtg
    42361 cgggaagggt ttaccccctc cgcttccttc ccgcgaacag gaggaggagg gccgccccgg
    42421 cggcgatcgc gccaaatccc ggtgttgtcg gtgccggctc cgcggcctcc tcgaggccgg
    42481 ccagccaggc ggcgcccgat gcgggcgaga ggtgccggat gcccgcaagg acgtagccgc
    42541 cgggggtgct ctccgctgcg tagaccctcg tatccgggat cgtcgtgttc caccggatcg
    42601 ccccgtcccc gtcggtcccg aaggcgaagg cctcccctct cctgatgtcg gtgcccgcta
    42661 tcatgtatcc cccggcaggc cgcagggtga cggcgtagcc ggcaagcccc tccatgttcc
    42721 ggagccagac ctcatcccct tttgcatcga cgaggccgta ccagaagttc cccgagtgga
    42781 caaacccgcc ctcgtcggtc tcttcaacat cgaagatgac cgggacggcg tagttcttct
    42841 cccagagcgt gttgccggca gtgtcgagac ggatgaggaa cgcatccccg atatcgtagg
    42901 cgaagggcga cttcgtgccg ccgatgatgt atcccccgtc agcagtcacg atccctgcgt
    42961 tcgcagcggc tccggggaat gtccggttcc aggtgggcgt gccgctgccg tcggtcttcg
    43021 tgacgatcgc ggtcgtatcg tttgccgatc cgggcgggtc ccagagccat ccggctatga
    43081 cgtagccgcc gtcggcggtc ggcctgaccg ctgagacctt ctcgccctgc aggacctgcc
    43141 gccactcttc cttcccggcg gcatcggtcc tgatcaggag cgagctgccc tggtaggcgt
    43201 tctgctccgg ttcggccgta gcgttgtatc cggcggcgat gtaaccgccg tcggcggtct
    43261 ctgcaaccga agctgccgcc atgccgggga acctctccgt ccatacttcg ctcccccggc
    43321 cgtcggtctt tacgagcagc agatcttccc cggacccggc gaggaccgag ccgagggcga
    43381 tataaccgcc gtccgccgtt cctgcgacgg cgtcgaactt gctgttctcc tcaggcgaga
    43441 acgttgcatt ccaggcgacc ggcagagcgg ccccgcttac cgggacgacg gcgatcatcg
    43501 ccaccgcgat aagcagcgcc gccatggtcg gcccccggtt ccggaagtat cggtatattc
    43561 ccgtcatgga atcgatcact ggtgcggttt aacacgccaa aaatttagtg gttctctctc
    43621 acccgggccg ggaggtgtgc cgatgcgccg gcgcgccggc aatcccttaa agtacacaaa
    43681 ccccctataa ctctaccacg tgttggcgac attcgacgga acctgggtgc gccttggtgg
    43741 cgaaggacgc acgctctacg agcagggggg gtacgggagg cccgagggaa ccggtctccg
    43801 gctctctccg gaagaggcgc tctacctcat ggagaggggc aagatcgacg tgaaagactt
    43861 cgacttcgac gccctgctcg gtctctttgc cggccagccg aattttgtcc gccggtatct
    43921 cgtctaccgc gacatccgcg aacggggata cgttgtccag cccggcccgc acgatttccg
    43981 cgtcttccgc cgcggccaca aacccggggt cggcaggtcc cagtacctga tccgtgtcct
    44041 ctccgagcgg gaccttgtcg atttcgagca gctgggccag gacgtgcttg ccgcggtcaa
    44101 catgaggaaa cagtacctcc ttgcggtcgt ggacgacgag gacgaactga cctactacga
    44161 ggtgcgggtg caggacctcc ccggcgtcgg ggagccggcc cggtgcgaca gaccgatcga
    44221 ggcctcgctc ttcggcacct acgcgcttgc ccacctgcct ccgggaaccc cgcttgagga
    44281 gggctggtac ggcaaacggc ttgaccccga gaggctcctc ttccgcccgg tcgagtcgat
    44341 ctacctcatg cagaaggggt gcctcgcggt cacgcgggat gggggtcgga tgaccgcgga
    44401 agagttccgc ggtgttgcag ccgaaaaaga catcgagatc cgcgagaaag agcgggtctt
    44461 ctccgatctc cgggagaaag gctacatacc gaggaccggg tacaagttcg gccaccactt
    44521 ccgggtctac tccggaaaga agacccactc cgagatgctc gtccacgccg tcccttccag
    44581 aacctccacg ccgatgagcg ctgtctcccg gtcggtcagg cttgctcaca gcgtcaaaaa
    44641 aaagatgttg tttgcctgca tatacaatag cgatatcgga tacatcgaat ttgcccgaat
    44701 caagctgtga gcgtcttttc atgcagtccg gaatgaatcc gtggtcaagt aatcagaccg
    44761 tcgatgtaga tcgactcttt gccgagttcg gcatcgagcc tgtcggtgag gtcgcacgag
    44821 ggcttcccga ggtaccgccg tttatgcgca ggggtgtcgt cgtcggacac cgggattacg
    44881 gcctgatagc cgatgccatc cggaaccgca ccccgtttca cgttctgacc gggttcatgc
    44941 cttcaggcct cccgcacctc ggacacctga tggtcatgaa ggaggtggtc tggcacgtcc
    45001 ggcagggcgg gaacggctac gtggccatcg ccgaccgcga ggcgcacgcg gtccgcggca
    45061 tatcgtggga gaagtgccgc gagtacggga aggagtacct caaagccctc tacgccctcg
    45121 ggttttgcgg cacgacctac taccagagcc gaaacgatcg gttaaaagac ctcgcgttcg
    45181 aggcgtccac gaaggtgaac ttctccgagc ttacggcgat atacgggttc gggcccgaga
    45241 cgtcgctctc gcatgccgtg agcgtcgtca cccaggtggc cgatatcctc ttcccgcagc
    45301 tcgacgccgg ccccgccccc acggtcgtcc cggtcggcct cgaccaggac ccgcacatcc
    45361 ggctcacccg tgacgtggcc tacaagctca ggatgttcac ggtcgaggac cgcggcgacc
    45421 acatcagcgt ccggtcgaag aatgccccgg aggcggctct cgaggccgta caccgcacgt
    45481 tcagcggctc aaagaagtac gcgggccacg tggacgtcac cggcgtgccg atggcgcgca
    45541 tcgaggatgc cgtccgcggg atagagattg cccgcggggg attcgggttc tatctcccct
    45601 cgtcgaccta ccacatattc atgcccggcc tccagggcgg gaagatgtcg agcagcgtgc
    45661 ccgagagcct ttttgggttc tacgagcccg aaccgtcggt cagaaagaag atcatgggcg
    45721 cgctcaccgg cgggcggatg acgctcgagg agcagaggcg cctcggaggg gagcccggcg
    45781 gctgcccggt cttcctctta aatctcttcc acatgctcga ggacgatcgg gaacttgccg
    45841 atctccgccg ccggtgcgag ggaggcgagc tcacctgcgg gcagtgcaag aaggagacat
    45901 atgaacgcgt gcaggcgttc cttcgtgagt tgcgggataa gatggatgca gtagagcacc
    45961 ttgcagagga ggtgtgaacg gtggaactga cacagaacga gaagaggctc ctggctgccc
    46021 tggggccggt gggatcggcc gatgcggccg cccttgccgg catgatggat gcccgccggg
    46081 aggcggtggt gcagtatgca aaccttgccg gcgagcgggg actggtggat gtcgagaagc
    46141 gcatctcccg acggtatgtt ccgaccgacg aggggcgggc ctgcgccggg aagggccttc
    46201 ccgagcggca ggtgtttgag agtttcggag aggccatccc gatgcgggac ctgcaggcgc
    46261 acccgcttgc aaagatagcc gtcggctgga tgcggaagaa gggctgggtc accatacggg
    46321 atggggtcgt gcagaagacc ggcaacgccg ctcccggacc cgacgagacc gtgtttgccc
    46381 gaatcagcga gaacggttcg gctgaagatg gccccggggt tgccgacctt ctccggcgcg
    46441 ggctcgtgga ggaggaggag accgtcacct acaccgtctc gatcacgcct cttggctgca
    46501 gcctcctcgc cggagggctt gacctcgagg aagaggcggg tacgctcacc cgcgaccaga
    46561 ttctctccgg tgcatggagg gatctccccc tccggcgcta cgacgtgacg aaacttcccc
    46621 ggcgcgccta ccccgggaag gtccacccct accagcgcct tctcgacgag atgcgccgta
    46681 tcctccttga catggggttc accgagatgg cgggcggtat cgtccagagc tcgttctgga
    46741 acttcgacgc cctcttccag ccgcaggatc acccggcgcg ggagatgcag gacaccttct
    46801 tcctcggcga gcgctggccg ctcccggcgg ggtatgagcg cgtccgcgat atgcacgagc
    46861 acgggggcga gacatcttcc gccggctggg ggggcacctg gagcgccgca aaggcagaac
    46921 agtgcgtgct ccggacccat acgacgggcc tctccatcca gcacctcgcg aagcacccga
    46981 aaccgcccgt acgggcgttc tgcatcggcc gggtctaccg gcgggaggcg gtcgacccca
    47041 cccaccttgc ggagttcgag cagctcgagg ggatcgtcat ggacgaaggc gtcaacttcc
    47101 gccacctcct cgggttctta aaggagtttt acgccaagat gggctttgag aaggtcaggt
    47161 tccgccccgg ctacttcccc tacaccgagc cctccgtgga gcccgaggtc tacgtcgaga
    47221 atctcggctg ggtggagctc ggcggggcgg gcatattccg gcaggaagtg accgcgccct
    47281 tcggcatcga gcacccggtc ctcgcgtggg gtcttggtat cagccgggtg gcgatgctcc
    47341 gtctcggcct tagggacctc cggcatctct acaggagcga cgtcgagtgg atcagggaga
    47401 cgcccaccta cggcgggagg cggtgatatg gctataatca cgttacccta ccggtacctg
    47461 gagcgattga ccgggaccga ccggcagacg atcatcgacc gcgttccgat gatcggggcc
    47521 gatatcgagc ggatcgagga agaccacgtg gacgtcgagt tcttcccgga ccgccccgac
    47581 ctctactctc ccgagggtgt tgcccgggcg atgcggggat tcctcggcat cgaggagggg
    47641 ttgccggtct acaccgtcca cccgtccggc ataacgttct cggtcgaccc gggccttgcc
    47701 ggcatccggc cgcaccttgc ctcagcggtg atccggaacg tcgacctcga cgaggaggcg
    47761 atcgagagcc tgatgagcct ccaggaggca ctccactggg cggtcgggcg cgggcgggcc
    47821 aaggtggcga tcggcgtcca cgacctcgat atggtcacgc cgcccttccg ctacatcgcc
    47881 tccccccgga gccggtcttt cgtcccgctg gacttcgacc gggagatgac gatggaggag
    47941 atcctcgtcg accacccgaa gggccgcgac tacgcccgcc tcgtggagga tttcccccgg
    48001 ttcccgctga tcgtcgatgc ggaagaccgg gtgctctcgt tcccccccat catcaacggc
    48061 gagttgacac gggtcacggc ggcgacgaag aatatcctgc tcgactgcac cggcaccgac
    48121 cgaaaagcgg tgatgacggc gacgaacatc atctgcaccg cgcttgccga ggccggggcg
    48181 acgatcgaga gcgtcaccgt cgcaggcgag gagatgccga cgcttgcccc cgcggagcgt
    48241 atcgtctcgg tcgatgagtg cgccgctctc ctcggcatcc ccctcacggg ggaggagatg
    48301 gtgcggctcc ttcgccggat gcggtttggg gccgaaccgg agggcgcgtc ccacctccgg
    48361 gtcttcgtcc cgtgttaccg ctcggacatc ctccacgact gggacatatt cgaggacgtg
    48421 gcgatcgcct acggctttga gaacttcgac gccgccctcc cgccgacgtc cacgaccgcc
    48481 gaggagcacc cggtcaccgc catagcggga gcggtccggt cggttatgat cggccttggg
    48541 tacctcgaga tgatgccctt caccctcacg aacgagcggg tgctctacgc gaacatgcag
    48601 cgcgagcccc tgcccgggac cctccgggtg ctccacccga tcagcgagga gcagaccgtt
    48661 gtccggaccg acctccttcc cctgctcatg gagatgctcc aggcgaacaa acatcgcgag
    48721 ctcccgcaac gcctctttgc ggtgggcgac gtggtcaggt actgtgtcac ctcccagatg
    48781 gccgcggcgg tgagcatcca tccggctgcc gacttttcgg aggcatacgc ggcggccgac
    48841 gtcctctgcc gggagctcgc gcttccctat acggtcgcgg agtcgtccga cccggcgttc
    48901 atcgacggcc gccgcggcga catcatcgtc gatgggaaaa ttgtgggggt gttcggggag
    48961 atccacccgg ccgccctgaa cgcgttcgac cttgagcatt cggtcgcagc gttcgcgctc
    49021 gaccttaccg ccgtaccggg ataccccgtc ccgccaggta ctccttgacc tcgcggatgg
    49081 cgaacacacc gtagtgaaag acgcttgccg cgaggcaggc gtcggctttt ccttctgcaa
    49141 acccttcgta aaagtgttcg agcgtcccga cgccgccgct tgcaaccacc ggtatgccaa
    49201 ccgccgtcgc gaccgccgcg gtgatcggga tatcgaaccc gtccttcgtc ccgtcggtct
    49261 ccatgctggt gaggaggatc tcgccggccc cgcgctcttc ggcctcgcgc gcccaggcga
    49321 cggcatcacg ccccgttgcc ctgctcccgc cgtgggtcac gacctcatac cagcactccc
    49381 ggccgtcggc gagggcgatc ggcttcttcc ccggttcaac cgcgtaattc cgccggacat
    49441 cgatggcgac gacgatcgcc tgcgcgccga accgttccgc cccccgggag atcaactccg
    49501 ggttctctac ggcgcttgag ttgatgctca ccttgtccgc acccgccccg aggatctgct
    49561 gcatgtcatc gatcgtccgt atgccgccgc cgacggtgaa cgggagggcg agctgatccg
    49621 aagcccgccg gatcacgtcg atgatagtgc cccggtgttc ttttgaggcg gctatatcga
    49681 ggaagaccaa ttcatccgca ccctgttcgt cgtagcgccg ggccagagcg acggggtcgc
    49741 ccgcgtcgca gaggtcggtg aaatgcgtgc ctttgacgac ccgcccgtcc ttgaggtcca
    49801 ggcaggggat gatccgcttc gtcggaacca tgcctactgg tgtgaggcga tgggagatga
    49861 ggatttcggg gggcgaccgg aagaacgggg ggtgggcatc gtcaggtgcg gggagatgcc
    49921 gacctgctcc cttcccgggg ccgcaccggt ccatctgcgg ccgcccctca ggtttaagta
    49981 atccgggagg atacctttac aggcaaaccg gaggagcatc cgggcgggat aacgggtctg
    50041 gtgattgaat ggagtctgga gatcttaaga ttgcatgggc gcggcagtac atgcccgtac
    50101 tctcgtccat tcgggaacgg tttatcgcgg agaaaccttt tgccggcatg accatcggca
    50161 tggccctcca cgtcgaggca aagactgcgg tcctggtcga gaccctggcg gcaggcggcg
    50221 cagaggtgta catcaccggg tgcaaccccc tttcgaccca ggacgacgtc tcggcggcgc
    50281 tcaacacccg taagggggtt cactcgtacg cccggcgcgg ggccgatgtc gaggagtact
    50341 acgcggccat cgaccgggtg ctcgacgccc ggcctgctgt caccatcgac gacggcatgg
    50401 acctgatcta ccggatccat accgagcggc gggaggtgct cgatgcggtc atcggcgggt
    50461 gcgaggagac cacgaccggc atccaccggc tccgcgccat ggcccgggac ggggcgcttc
    50521 ggttcccggt catcgccgtc aacgatacgc cgatgaagca cttcttcgac aacgtccacg
    50581 gcaccgggga gagctcgctt gcgtcgatca tgatcaccac aaacgtcctc atcgccggca
    50641 aacacttcgt cgtcgccggg tacggctact gcggccgggg gcttgcacag aaggcgcgga
    50701 gcctcggtgc ccgcgtcatc gtgaccgagg tcgacccccg gagggcgctc caggcccaca
    50761 tggatggatt cgacgtcatg acgatggatg atgcggcacc cctcggcgac atattcgtca
    50821 ccaccaccgg caacgcaggc atcatcacgg gccggcactt cccgaacctg aaggatgggg
    50881 ccatactcgc gaacgccggt cacttcaacg tcgagatcga tgtcgagtgg ctcgcgtcgc
    50941 atgcggactc caccgttcac cgcgacggca tcgataccta cgtgctcggc ggcagggcca
    51001 tccacgtcct cgccgagggg aggctcgtga acctcgcaac cccgaagggg atgggccacc
    51061 ccatcgaggt gatggacctg agtttcgcga tccaggctct ttcggcggag ttcatagcaa
    51121 aacgcggcag agaccttgcc cccggtgtgc acgatgtgcc ggccgtcatc gatgaggggg
    51181 ttgcacggct gaaacttgcc gcgctcggtc tctcgatcga ccgcctgacg cccgagcagg
    51241 agacatatat gagcagctgg acgatcggga cgtgaatccg cccttccccc tttttattta
    51301 cggcgggtgg cgctgtcatc gtcgatcctg cgcataaatc cgttcccgca cctgcgacat
    51361 cggtactgca catacaggcc atccccggaa ataacgactt ctccgaggag ttctcccggt
    51421 gacccgcaca gggggcatac cggcctttta ccatcgaacc agcggccgca ccggaagcac
    51481 tggtgagggg tgtgtacctc cggaccgacg gcgatggcat cctcgacgtg gcgccgatac
    51541 ccgcagaagg gacagggagc gcttgcatcg gccccgttgc catccggtgg gcatgcgatc
    51601 cccgacctcc gatccgatcc gcgcatacgg caactcctcc agcttcgcac gaagtcgatc
    51661 tcatttaatg gggtgtcttt tggcatttca acctcccatc cggggttttc ggcagatcga
    51721 aatcggtcaa cccgctctgt ctcatacatc ggtgttgggt taccgacggc ttaatgatac
    51781 ctatgggtat ctcatggtat ctcgccggaa aaataaaaac aatgtaatat aatcgttcaa
    51841 acggaatctc tgaatgaaat aatgggtctc tccgacagcg tctcaagcaa gcaggtgggc
    51901 gtacgactgc ccgggcacct ctaccggtgg ctgaagaata aggtcgatag cggcgagtac
    51961 tcgaatatgg cccaatccgt catcggggag ctgacgaagg tcaagacgct cgaagaggct
    52021 cgatgtcagg agaccacatc gcacggtact tacgatgacg aacccctctc ccggatggtg
    52081 aacgagcgca tcgagggagt tcgccgcgag ctcctggatg aggtgaagcg gcggcgcgcc
    52141 tgatccgggc ccgtgcataa ccgccgcgtg ggcgaaggag atgcaccctt gccgatcggg
    52201 acctgtccgg tgctcgttcc cgccttcgat ggggatgccg cccggttctc tctcccgggg
    52261 atcacggcta cgctccgaag gcgacatccc gtggcaagca tgggaccgtg cttcccgggc
    52321 cgccgtcaac cggcccattg cattctgctc ccccacacgt taggagaggt gacggccgga
    52381 caatggtagg ggctccggca ggctccgctc ccccacacac gggggagagg ggccgcacag
    52441 gttgccggtg ctgcacagag gcctccgctc ccccacacgt taggagaggc ggcagcgcaa
    52501 gcctggaaag agcatggtgc cgcctacggc gtcgtgcccc cggcgagagg aggggtagtt
    52561 cacaactccc tctcgccccg gcaccgatct ccctccggcg ggatccttcc ccatggccgc
    52621 accgtgcctt tttaataccg gtatggaagg taagtccgtt ttagttgata tacgggcaaa
    52681 gtaaacaaca taaatacccg gaactgtaat cccctatacg aatggtgtta ttatgagatc
    52741 gtcaatcccg gcaactcttg ttgcagtaag ccttctcgtc atcgccggcc ttctgactgc
    52801 agggtgtaca accaccgagg aaacccccgg gccgaccacc ctgaccgtct tcacggccgc
    52861 atcgctcaca ggggccttca cggatatcgg cagggcctac gaggcacaga acgcagacat
    52921 caaggtcgac ttcgtctttg acggttcaca gaccctccgc acccagatcg agcagggggc
    52981 gacccccgac atattcgtct ccgcgagcac gaagcacatg aaagccctcc aggacggcgg
    53041 gttcatggac aacagcaccg tggcgacgtt cctcgggaac agtctcgccc tgatcgtccc
    53101 ggtcgacaat ccggcaggga tcaccagcct tgcagacctg gacgaaccgg gcgtgaagct
    53161 cgtcatcggc accaaggacg ttcccttcgg cgactatacc cggcagatgt tcgagaagat
    53221 ggccgccgat ccggcctacg gtcaggccta ccaggatgcc gtgatgaaga acgtcgtctc
    53281 cgaggagatg gcggtgagct ccgtagtgcc caaactggcg ctcggcgagg cggatgcggc
    53341 gatcgtctac aagtcggatg tctcaaaaga cgacctgacg aaagtgaccc ggatcggcat
    53401 accggccgag tacaacgtcg tggccaccta tccgctcggg gtccttgcgg aatcaccgag
    53461 caaggccgaa gcagaatcgt tcatagcatt cgtccggggc ccggacggca gcgccgtact
    53521 gacagactat ggatttgacc cgattccggc aggaaactaa cgtattcgag tacggcagcc
    53581 ggacgttatt gaggagggca accctcaccc tcctcaccgc cgtccttctt gcactcttcc
    53641 ttctcttcat cacgctgccg gttgtctcgc tcttcctgcg catatcgccg gaagcgttcc
    53701 tccgctcgct cgccgaaccg gtggtcctcg atgcgctctc cctctccttc atcaccgcga
    53761 cggtgagcac ggcgatcgtc gtcgtcttcg ggacgccgct tgccctggtg aacgcccgga
    53821 gcgactacgc ggggagggaa ctcgtcgata ccctcaccga tctccccatc gtcctcccgc
    53881 cggcggtggc ggggctcgcg ctcttgatgg ctttcggccg ccggggcgtg atcgggcagt
    53941 acctcgacat cttcggcatc catatcggtt tcaccacgct cgcggtggtc atggcccagg
    54001 tctttgttgc ctcgccgttc tacatcaggc aggcgcgggc gagcttcgag gccgtcgacc
    54061 ggctctacga ggatgcggcg cggaccctcg gcgcatcccg ccggaccgtc gccctccgga
    54121 tcacgatccc gcttgcgtgg ggcggactcg tctccggggc gatactctcg tttgcgcggg
    54181 cgctcggtga gttcggcgcc acgatcatgt ttgccgggaa cttccagggc cggacccaga
    54241 cgatgccgct tgcgatctat acgacgatgc agggagacat ggacgcctcg atcagccttg
    54301 caatcattct cgtcgtgata tcgttcgcgg tgatcgccgc ggtgaaggtc gtcacccgga
    54361 ggagcgcctg atgctctcca tccacgtcgg ccggcaactg cgggatttcg ccatcgacgt
    54421 cgcgctctcg gttcggtgcg gcgagaccct tgtccttatc ggggagaacg gggcgggcaa
    54481 gtccacggtg ctgaacctga tatcgggcat ccttgcgccg gaccggggcg atatcaccct
    54541 cggggagagg acgcttttct cgtctgcggc gcggatcgat atccctgccg agagcaggaa
    54601 catcggccac ctcttccaat cctacgccct cttcccgcac atgaccgtcg ctgagaacgt
    54661 ggcgttcggc ctccggtgcc ggaaggtgcc gaaacctgag attgccggcg ccgtcgcgga
    54721 gcaactccgg tcgatgcacc tctcggacct cgcggacgtc aacgtcggcc gtctctcggg
    54781 agggcagcgg cagcgggtgg ccctcgcccg ggctctggtg ctcgaaccgg aactcctcct
    54841 cctcgacgag ccgcttgcgg ccgtcgacat gcgtgcccgg ggagcgatgc gagaggaact
    54901 ccgcgaccgg atacggcacg ccggcatccc ctgcatcgtc gtcacccaca cccttcacga
    54961 tgttctgggg ctcgccgatc gcctctgtct catagaggag gggagggtgg cgaccgaggg
    55021 aacgccggag gaagtgctcg ggatgcggga gaacgggttc atcgcgagtt tcatcgaggg
    55081 gtgagcgaag agtggagccc gaactcccgc tgaagctagg cgtatgaagt tccatattcg
    55141 cacggtggtg gctcccttca ccattattcg gcttgttccc tcattttatc tcattttgca
    55201 agaccttgcc ccacctcccc tcccctaact ttttcctctc ccatcctcca aaccgtggtc
    55261 ggggtgacgg agaatctcac gtgccggcgc acctccctcc gaaacgtttg tccggctgaa
    55321 gctggaccgt tgttaccata gcccaccata gggcggtatg ggccataaag actacttcct
    55381 gctgttccgt tcgatggagg aaggaagacg gtgaagagag ggagatccgg gtagtatgcc
    55441 tgatgccggg tgtatgttgc cagcgtaaaa tacaggtgga aggctccgtt tttctctccc
    55501 ggcgtgcgca ggggtctcct taccccgccc ccttcaccct cgcaagaaca aatcaatgag
    55561 ttcttgttta gtcatattgt tatggtttgc gatat
//
LOCUS       DAOSII010000002        47587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_887_length_47587_cov_47.058361, whole genome shotgun sequence.
ACCESSION   DAOSII010000002 DAOSII010000000
VERSION     DAOSII010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 47587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47587
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_887_length_47587_cov_47.058361"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..504)
                     /locus_tag="PLV90_00265"
     CDS             complement(187..504)
                     /locus_tag="PLV90_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95573.1"
                     /translation="MTISKKIIFLTLALILVISVFGLLLQAYYHAYYNSEDDCGMKSP
                     PIPDPQDEKRAEIAAKLWKQNISLGEYMEQVYPEFFYNMSEECKEQYYNKPMNWPDIR
                     QGY"
     gene            929..2392
                     /locus_tag="PLV90_00270"
     CDS             929..2392
                     /locus_tag="PLV90_00270"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HOF95574.1"
                     /translation="MESLRIVGNPGFTPEVRPEWLTRGALSCVLDRFFAAGCEHLFTS
                     LALRVYTAFDIPFYPILHGDTTSISLYSTYEGAEDSPPSAPTICRGHSKEKRGDLVQI
                     MLGQVCDSHGIPLITTVHDGNEADCTWNADVIQTLAELDPFSRTDVTYIADAKLATAP
                     NSRKLLKKGFQFVTRCPANFSKKMAARVTRAAYEADAWLPIGSYRDTDKDTLETYDMQ
                     EFTQTTDDRISCRFLVFRGSHRRDEFEADLVTKREEFVAILKEMTRKRFACEPDAQQA
                     LEEARKNLRRYPLWNADLTVETVITEKNPRGRPGKNPRPTIQVTAWVIRASEPQLQQK
                     VYLAERRKAESFVLMTNVSEEDTPAREVLRLYKGQKTVEDNFSVIRRPTVVDTVYLKK
                     PERVAALVTLLAVALLIQAVIRVLVRRNLDALPEPPGLDHGCKPLIRPGLKKILRFIG
                     YHSLITTGDERRFWCISPDHEKNLGIWLRLLEFNVDG"
     gene            2721..3044
                     /locus_tag="PLV90_00275"
     CDS             2721..3044
                     /locus_tag="PLV90_00275"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95575.1"
                     /translation="MIISVMGATGGLMPVRDERIPEGLPMSAGYTPGVFIGTDDGFKA
                     VVGGKGGGTARVAQDLVEGLTEAQAVALAKKVIAFYRENGQAPERLGATIDRVGFDKF
                     KNAVL"
     gene            complement(3241..3987)
                     /locus_tag="PLV90_00280"
     CDS             complement(3241..3987)
                     /locus_tag="PLV90_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome assembly chaperone family protein"
                     /protein_id="HOF95576.1"
                     /translation="MEDVVVTFFRDDAGGAPVLVEGLPGIGHVGKLVADHLIDELGGE
                     KIAEISSVHFPPQVVVDEQGVTHLVNNEIYRCEKDGVAILFLVGDFQSTSAEGHYVLA
                     ERYLDLAEALGVRRIYTLGGYGVGHLVEHPRVLSAINMERLRPEVEAAGGSFENAGSG
                     GMIMGASGLLLGLGKARGIEGICLMGETSGYIVDPRSADSLLTVLSAMIGIDIDHTTL
                     QQRAEAMEQIIANIRETEEARGREELNYIG"
     gene            complement(3969..4136)
                     /locus_tag="PLV90_00285"
     CDS             complement(3969..4136)
                     /locus_tag="PLV90_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-protein complex protein Nop10"
                     /protein_id="HOF95577.1"
                     /translation="MSNRIRRCPDDLRYTLAQVCPICGRSTRPAHPARFSHEDRHGDY
                     RRVMRRWKTSS"
     gene            complement(4143..4922)
                     /locus_tag="PLV90_00290"
     CDS             complement(4143..4922)
                     /locus_tag="PLV90_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     alpha"
                     /protein_id="HOF95578.1"
                     /translation="MHEREWPEEGELVVCTVADVKDFAAFVTLDEYDGRRGLIPISEI
                     ARGWIKYIRDYIREGQKVVCKVLGVDPGRGHIDLSLKDVNEHQRREKIHEWKNEQKAE
                     KWIGFASESAGVDRKVIEESIYREYGLLYPALEDIVTTGGEAAERLGLDEPVKEALIT
                     IANENVKVSKVTITGHLVLTSPRPDGVNVIRRALRSAQPKIEDVDIDLIYIGAPKYRI
                     KVTAPDYKAAERAIEKAASAAVGVVERAGGSGKFVRKQKAG"
     gene            complement(4930..5118)
                     /locus_tag="PLV90_00295"
     CDS             complement(4930..5118)
                     /locus_tag="PLV90_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27e"
                     /protein_id="HOF95579.1"
                     /translation="MVRLNRENRSTFLRVKCPDCENEQIVFERASTVVACSVCGRILA
                     EPHGGKADIKADILAVLE"
     gene            complement(5127..5405)
                     /locus_tag="PLV90_00300"
     CDS             complement(5127..5405)
                     /locus_tag="PLV90_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L44e"
                     /protein_id="HOF95580.1"
                     /translation="MKMPSKFKTYCPFCRNHQTHEVVKVKRGKVRHFNWIDRQKDRRS
                     MVGNMGKFSKVPGGDKPTKKINVKYRCTVCGKAHLRPGFRIGKFELTE"
     gene            complement(5443..6105)
                     /locus_tag="PLV90_00305"
     CDS             complement(5443..6105)
                     /locus_tag="PLV90_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95581.1"
                     /translation="MAADPLERLRQTLLEMHHSGRLSDIEPALYEGARAYVEKLKADY
                     YGLPNPLESQSGSLLIEEIGSITETVQEIFAIRTRRILDLAFQQIEGQYFDRGEARKM
                     LPAERAMYGQIIDAVEGCRETLIRGEEGSFQGGGAAAVDAIEEPDIPPAAGAGRPAPT
                     IPRPLSSPYALVHIRSDVESFMGVDGRVYVLTQGDIVTLPENNADVLCEHDIALNIRL
                     NK"
     gene            complement(6109..7224)
                     /locus_tag="PLV90_00310"
     CDS             complement(6109..7224)
                     /locus_tag="PLV90_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase small subunit PriS"
                     /protein_id="HOF95582.1"
                     /translation="MKPATIEFVKQRFAEYYQRQTLAAPSSLEQREWGFVFFDAASAV
                     RMRRHMAFADPLELTAYVRSLIPAHAYYSTAYYQTPSAPTMNDKHWAGADLIFDLDAD
                     QIVRGPYAMMLARVKEETVKLVGMLTDELGFARNHIRIAFSGGRGYHIHVTDIAVRGW
                     ESPERREVVDYVCGIGLDPGVMLAPGRTPAGWRWRYIEALRDHLLWLEGLDAAEAAAY
                     LTGPAGIGKRAAAEFLAGLGALREKDPGTLRENRIVRAIMGTPAFEERVRGAGARADE
                     PVTTDIKRLIRAPGSLHGGSGMRVVPLDSRDLAEFDPLVDAVVFGDREVRVDLQMNVA
                     TSLLGNTYTLKTGPASVPEALAVFLCCRSMAEIAGGA"
     gene            complement(7221..8795)
                     /locus_tag="PLV90_00315"
     CDS             complement(7221..8795)
                     /locus_tag="PLV90_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HOF95583.1"
                     /translation="MNDTDTLREIISRILSAGPDPADIQRIKLEVCREHGANMPRNSA
                     VLAAATPDERERLRPLLLVKPTRTLSGVAPVAVMTSPHPCPHGKCLPCPGGPEHPFGS
                     PQSYTGEEPAALRAREHAFDPYRQVQARLGQFEALGHHVDKAELIVMGGTMTARPVEY
                     QEWFVGAAVQAMNDYPRAGVPPEKPDLDAIFAANERAGVRCIATTFETRPDWSRAEHI
                     DRMLAMGVTKVELGVQQIDDRILDYNRRGHLVADSVAANCLLRDAGLKVGFHVMPNLP
                     GASMEDDRRMFRELFADPRFRPDFLKIYPTLVTPASEIEALWERGDYRPYTEEELVDL
                     VAYAKSLLPEYVRLQRIQRDIPAKLIVAGSRHSNFRQLAAARLHSQGLRCRCIRCREV
                     GRSVAPEETALSTVTYPSCGGEERFIQAGSEDALVGFARLRFPHRPFRDELAGAALLR
                     ELHVYGSVVPLGTPAAADEWQHRSYGVRLLAAAEEEASGHGYGRLAVMSGVGVRPYYR
                     KQGYERVGPYMIKTIS"
     gene            complement(8799..9065)
                     /locus_tag="PLV90_00320"
     CDS             complement(8799..9065)
                     /locus_tag="PLV90_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0058 family protein"
                     /protein_id="HOF95584.1"
                     /translation="MQKEELLHLHMLLVHVRKYYESTTGEDVSTDQYNALHISPVHIH
                     KNKVTHKKAILTLGNEIVQHIRANHNPYIDYHADFPSGRIATEH"
     gene            9133..10005
                     /locus_tag="PLV90_00325"
     CDS             9133..10005
                     /locus_tag="PLV90_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOF95585.1"
                     /translation="MHKVRAVGAFIGLAIGDALGAPLEGFPPPPFTVTEMEGGGMHHT
                     LPGQYTDDTLQASALAETLVRCGGLDPGDFSRRLISAYRARPEFFGPTSRAVLDLVEG
                     GVRPEDAARMVHDARGGSRSNGSVMRGIPVGIFYPPDVVREASLAASRVTHFDAAAGE
                     ASAFVNRMVSGMCRGEEASAAFGYALAACRDPELHGLLRDWRAHPREPSLDAVLCTHC
                     AVSVFMDAPTFRDAVITAVNLGGDADTVGAIAGGLAGACWGLHAIPGSWLSALQDREM
                     LLDLARRLSQAGRP"
     gene            complement(10136..10588)
                     /locus_tag="PLV90_00330"
     CDS             complement(10136..10588)
                     /locus_tag="PLV90_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0179 family protein"
                     /protein_id="HOF95586.1"
                     /translation="MVKPKTKVTLIGAALAKPGLDFVYEGNVCTECESCKVRKTCHNL
                     QPGKKYRVAAIRSNTRQDCPVHHEAVVAVDVLEAPIVALIGADMAIANSKISYEFSCP
                     RTTCRSYRLCRPDGIIEGEKYIVGEVLGNAPDVCERGRPLKLVELRPI"
     gene            complement(10597..11679)
                     /locus_tag="PLV90_00335"
     CDS             complement(10597..11679)
                     /locus_tag="PLV90_00335"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-1-phosphate
                     dehydrogenase"
                     /protein_id="HOF95587.1"
                     /translation="MSADGINLLRTKVFDKSKWMQLPRDVVIGHDVIEQLPTVCEDLA
                     LGDSVLIISGDRTRDLAGRRVEALLAGSYDVATCAADPNNPVETIKRAEASAAKAGFV
                     IGVGGGRVIDTAKIASYNTDRHFISVPTAASHDGIASSRASVPTADGNVSLAAEPPIA
                     VVADTAIIASAPHRLLASGCADIMANHTAILDWELSHRLRGEALSEYALTLSRMTAEI
                     LFKNADLIKPHSEESAWIVTKALVSSGVAMSIAGSSRPASGGEHKFSHALDRLAPGKG
                     LHGEKCGIGAIITMYLHGGDWEGIRDSLKKVGAPTTPAEIGIDDETAVEALLAARTIR
                     PERFTILDMGLTAASARDLVKMLYRE"
     gene            11691..12536
                     /locus_tag="PLV90_00340"
     CDS             11691..12536
                     /locus_tag="PLV90_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF63 family protein"
                     /protein_id="HOF95588.1"
                     /translation="MIREFLYKYYIDPIRYGQPYTLVDTLTYALILIAAVYIVYRGLR
                     RYKIAVDDELVLATMPFVVLGGLLRVVEDTGMIASDFRFLLITPLIFFTIFAVAAVSL
                     FLGKLAENAGFVARYSRVYGGAGIAACIVAGAALVWFGLTETKIALDVLAAILALASV
                     SSLALWALLAYGAKWDYASNIFYKLLIFGHMLDASATSYGIEIHPIHYVEQHVVGSGL
                     IEATGTAFSMFLLKIVVLIPAIYILEQYRREGNPGLWHLILLAMITVGMAPGVRDMVR
                     MVLYV"
     gene            12529..13095
                     /locus_tag="PLV90_00345"
     CDS             12529..13095
                     /locus_tag="PLV90_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HOF95589.1"
                     /translation="MFERPIVGATIVAAALFLVSVGLGIFAVIQDPSTGAQVMTLFRD
                     QVVDQLLSDSPAVLAGKIFVNNLSTCLLLFLGGASLGIVTLIVLTVNGLLIGAVAELV
                     RQQQGLLFIAAALAPHGIFEIPSFLIAGGLGFLLGQELIAEWHGRGDAAAGALPLARL
                     FLRVVVPLLAVAAIVEAFITPAVLSVIT"
     gene            13132..14565
                     /gene="proS"
                     /locus_tag="PLV90_00350"
     CDS             13132..14565
                     /gene="proS"
                     /locus_tag="PLV90_00350"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOF95590.1"
                     /translation="MEEDTGALPRKDDFSEWYNEVLWRAEIMDVRYPVKGLYVWYPHG
                     FAIRKRAYGILRDLMDRDHAETMFPLLIPETEFMKEATHIKGFEDEVYWVTHGGRNEL
                     DVPLALRPTSETAIYPMYSLWIRSHTDLPLKLYQIVNTFRYETKHTRPLIRLREITSF
                     KEAHTVHATREEAEAQVETALGLYREFYESLRVPVIISRRPAWDKFPGADYTIAVDTI
                     MPDGKTLQIGTAHMLGDHFSRTYEITYEDANGERQYASQTCYGISERSIAAVISVHGD
                     DKGLVLPPEVAPIEIVVVPIIVGKRRDEVLLAARALEEELRSAGFVVKLDDRDLRPGA
                     KYYHWEMRGVPLRLEIGPRDIDANTVVAVTRDGHKITLDRRGVVEGVFSALAAFRKGL
                     EQTARQAMIDRITVAATLEETVLAVRKGVAVVHWCGSKECAEEIEAAVDASILGSDIR
                     SDLITVSRGPCIACGGDGTSTLVARTY"
     gene            complement(14566..15021)
                     /locus_tag="PLV90_00355"
     CDS             complement(14566..15021)
                     /locus_tag="PLV90_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95591.1"
                     /translation="MTSRMHTPHTTCPGCHEEVFLDELVGGRCPLCGYSLDEDEGVCS
                     EYEETLERSDLGWMILQFFIFRRFCNEGANPLQVMQVLSRYEELAQCNPADAEKMQFT
                     LEVPMSRWERLLPKRCSKCGRIFFSGGKAAISGDISSPEHPREYTCPSC"
     gene            15380..16300
                     /locus_tag="PLV90_00360"
     CDS             15380..16300
                     /locus_tag="PLV90_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOF95592.1"
                     /translation="MASPAASEVYIIDASDRDHAIEALWREVGFPPLDGKDIAVKANF
                     NSDDPFPATTHPDTLGAIFGQLLDAGARTIRLGERSGMGETRAVLRNRGAVDVAARAG
                     ADVVALDDLPREGWEAVPGDGLHWREGFLIARLFWDADVVVQTCCLKTHRFGGHVSLS
                     LKNPVGAVAEKNPGGGYNYMMELHASPHQRRMIAEVNRFFPCALSVVDAAEGFSTGGP
                     ERGHRIAPGVILASTDRVALDAAGVALLRLYGSTPDVMRGRIFAMDQIARAAELGVGV
                     RSAQDLRLVALDAESKETAFDMRRILDGAG"
     gene            complement(16297..16848)
                     /locus_tag="PLV90_00365"
     CDS             complement(16297..16848)
                     /locus_tag="PLV90_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOF95593.1"
                     /translation="MEFATTLLIAVGLAMDAFAVAASGGTTVREARFRYALVVGALFG
                     GFQAGMPVLGWLGGAGLASFVGTYDRWIAFLLLALVGGRMIAGAVRGDGEAVQLATVG
                     IATLLLLAVATSIDAFAVGVTFAVLGTPVLWPAVVIGIVTFALSAAGVLIGSTLGRAF
                     GQKVEVLGGAILIGIGLRILLGL"
     gene            16940..18847
                     /locus_tag="PLV90_00370"
     CDS             16940..18847
                     /locus_tag="PLV90_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOF95594.1"
                     /translation="MSTQKIGLIQTAVGDDLDRNLAHALALTKQAIAAGARVICLQEL
                     YRTPYFPQYEGRDASFYAETIPGASTDAFSALAREHGVVIVVPLYERTADGEFYNTAV
                     VVDADGHLLPPYRKVHIPYDPLFYEKTYFRPGDRYRVYDTRYGRIAVLICYDQWFPEA
                     ARAVALQGAEIVFYPTAIGRIAGEEEPPEGDWRDAWETVQRGHAIANSVHVAAVNRVG
                     SEGDIRFFGSSFVADAFGNVLARAGETAEEVLVVEVDLAGNEAVREGWGFFRNRRPET
                     YGAITRRFLPAKTPQALGYRMPAEWEPHDAVWLSWPHDRETFSDLAAVERAYVAIIAA
                     LRGSETVDLLVTSDMMRTRIKAMLEEEGADTGHVRFHIAEYADVWFRDYGPTFVVDRR
                     TGGLAMVNWTFNAWGEKYPELMGDTRIPLIMNREMNLPLFTPGIVMEGGSIDPNGRGT
                     VLTTEACLLNPNRNPHLSREEIEAYLEAYLDAGHVIWLKQGIAGDDTDGHIDDVARFV
                     GPTTVLCALEENEDDENYAVLQENYAILRASTDQDGNPLTVIPLPMPGAVDGEDRLPA
                     SYANFYIGNTVVLVPVFGHPNDETALARIRQAFPDREVVGIDCTAMVAGFGAIHCVSQ
                     QQPSVQRPESE"
     gene            complement(18858..19367)
                     /locus_tag="PLV90_00375"
     CDS             complement(18858..19367)
                     /locus_tag="PLV90_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOF95595.1"
                     /translation="MDALEAIMTRRSVREYTAGPVPGETVRRLLAAAMQAPFAGDRPP
                     WHFVVIDDPRILARIPMIGLYARVSPPPPLAILISTDLAPPESPGLQVQGSAAAAQNL
                     LLAAHALGLGAVWTAVYPDADRMLGFANLFSLPGTVIPFALVTVGHPAARPPPVDRYS
                     EDRVHYNGW"
     gene            complement(19383..20102)
                     /locus_tag="PLV90_00380"
     CDS             complement(19383..20102)
                     /locus_tag="PLV90_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOF95596.1"
                     /translation="MDPEILSIGLLLAAVLAAGCAGNLLLPGKNSTPAPPDDAVILPA
                     PRSEGGVSVEEALWKRRSARIYAEVPLMLADVGQVLWAAQGITNDRGYRTTPSAGALY
                     PLEVYVVAGSVVDLEAGVYRYRPDGHLLVRIGGGDRRADLQAAAVDQASVGDAPATLV
                     IAAVPERTTAKYGERGTRYVAMEAGHAAENVYLQAAALNLGTVAIGAFDDDRVEEILM
                     LPKNTTPLYLMPVGRPVPGND"
     gene            complement(20213..21685)
                     /locus_tag="PLV90_00385"
     CDS             complement(20213..21685)
                     /locus_tag="PLV90_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF95597.1"
                     /translation="MVFETKESLTDEEIRRGLKLVIRDGLATQAMVTLTGGIFLVAFA
                     LQLGASNTVIGLLAAIPPLAELLQMPAIYVVDRVQKRRLVVVAASLAARLCWLPIVLI
                     PFFFSPEQGIFVLIASIALYASFSAISHCGWNSWMRDLIPQDRLGAFFSRRLTLSTAL
                     ALVISLAAGFFIDSWKTVFPDLAAYGYSALFLFGLVAGLFGITLLARTPEPRMVAADE
                     EDGLLATIKKPFTDVNFKNLIIFLGSWNFAVNLAAPFFTVYMLQTIGLDISVVVALGV
                     GSQIMNIVFYRSWGRISDRYSNKSVLAVSGPLFMLAIFAWMFVTLPDVYILTYPLLVL
                     IHILMGVSLAGVSLASGNISLKMAPRGQATGYLAASTFTNSVAAGIAPILGGLFVDFF
                     AERELLWTLIWKSPVRELVFVTLDLRQWEFFFLFAFILGLYSLHRLAAVQEEGEAKEQ
                     EVVDALIAGVRRDMRNFSPAGGLRDLVKFPFSSARGRKKR"
     gene            complement(21925..22737)
                     /locus_tag="PLV90_00390"
     CDS             complement(21925..22737)
                     /locus_tag="PLV90_00390"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOF95598.1"
                     /translation="MRRFEGKNVLVTGGSTGIGRATAIRFADEGANVAINYYASETEA
                     EITLEEARKTCSIIRGKGCGEMLVQGDVAHEEDVKRIFREVLGAWGRLDILVNNAGVQ
                     VASPAHETDMDAYDRVLAVNLRGAFLCAREAVRHFLDRGGGGVVLNTTSVHETIPKPY
                     YAPYVASKAGLAALSRTLALEYAGQGIRVNTVAPGAIATPMNREWMDDPARRAEVEKH
                     IPVGRAGLPEEVAAVFAFLASDEAAYITGQTLYVDGGLTLYPDFRVPWSSGS"
     gene            23061..23447
                     /locus_tag="PLV90_00395"
     CDS             23061..23447
                     /locus_tag="PLV90_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOF95599.1"
                     /translation="MTGAGDGELRFLVVEDDGIIAFDIVSRLKELGCGDVSVAATGAA
                     AIRQAERLQPHLIFMDIALKGGMDGIEAAEVIRDRFGIPIVYVTAHSDPAVRNRAMAT
                     SPAGYIVKPFTTADLVCAIRSALRTP"
     gene            complement(23422..24654)
                     /locus_tag="PLV90_00400"
     CDS             complement(23422..24654)
                     /locus_tag="PLV90_00400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HOF95600.1"
                     /translation="MPDEISELEEIKMLLRQEPLGLSILEISRRLNIGRNVTAKYLSM
                     LYAAGQVDLRRVAAAKLYTLAHRVPVSALLGLTSDLIVVLDRNLRVVLANDAFQALLR
                     LDRTELVGRQIGAFPPDRLPPPEIGERARVALRGQEVKCEIPWKADGRTVQFHATFVP
                     VVFEDGSPGVTAILEDVTREVEAAQDLEKALEEKEALINAIHHRLRNTLQVVSSILHL
                     QTTCLTDPAARRAIRATELQILSLALAHEDLYRSQYPDRVRMEEYLTRLTGTLLGSYG
                     VPPEKIAWKVRAPGIEMHLELAQFIGFLLTELVVNVIQHAFPDGMTGTMEVTIDRSRA
                     GRYTVTVRDTGVGISEEMHITATAAPGLPTVRLLVEEYLKGHVAVEWSGGTTVTVTFG
                     EEDMLVASPEATESEGQT"
     gene            25505..27901
                     /locus_tag="PLV90_00405"
     CDS             25505..27901
                     /locus_tag="PLV90_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HOF95601.1"
                     /translation="MELLDSAAAIVSGTVAGGARVYVFLQRLGKAVGVGRVCLAGVEY
                     RPKAGEVRLRILCEWTAGGIAGKEEGGQSVAYQPLPPGWAKRLMAGFPVHGRFPASPG
                     SVLMVPVFCGSELRGILEFRSRVARRGWRRSDVGTLKAVAGILGSVMAGSGAPHAVGA
                     CSCAVVDDPTVPFCRLSPDGAIAYASDSFCALFYRARDDVIGISVSALFSPEIMKKLP
                     NVAGFMVTPEFRAPGADGGKHCCRSVCRTLLDDRGAVCGYRVFGCKSTEFKQQEVRSI
                     GTDDRMFTLLAEAVYDAVVVLDRSARITYWNRAAEDLFGYTADEVVGHDAGQLFSPEH
                     VHLLYLEGFKHAVAAAAEGVVRLRFPDVSLVRKGGAVFPAALSIAAVLTRGAWFGIGV
                     VRDTTAEKIREQAILTEAERIGTLLRIAARLNGGVPFDAALDLLCEETARALRVSGAV
                     IYLYDLPGDLLAPARSFGVPEEVRKSLPPIPQALYEREVERQGSAFVIPDKRPWTDVA
                     SGKKMPGNPLRPLASAAILHGRTLIGALNVMTLDTPRSLSPEELTLLKGIADLAALAI
                     ADIRFSAERAGHEHQLQALLDEKTALLKEVHHRVKNNMQVIASLLSLQGRLTENAVAR
                     ACIRESENRVKSLALAHENFYRSESIAAVDAGDYLLRLVNDLLRLYAPPVHVSVDVET
                     EKGLVLPGDVAIPLGLLLNELVSNSLRHGFAGRSEGVIAIRIVRATNRRLTIEYRDDG
                     AGIPGEFAARPPATLGLQLVRILAAQLEGTVTFRRGEPGTIVEITIGDGAGEGRMNHG
                     "
     gene            27894..28922
                     /locus_tag="PLV90_00410"
     CDS             27894..28922
                     /locus_tag="PLV90_00410"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOF95602.1"
                     /translation="MDSPSDTTEGHTVLLADPDPLTAATLSLRLQELGYAVVGAAAKS
                     DGALAAAAAFRPDVVIISADLPGPGSPRETALGIRDGLGIPILVTAAHPVDTLSPDGE
                     EGLSPQEILVGLFGPDDLACAIEAVIARHRARHPCREWVEAFRQAAEIVPGFIMLLGT
                     DRHIRFVNLSGAAMAGRPRDALIGLDAVGTALPLSDRDGGDLRRRFDEEQEFHVAADC
                     IPIGRCRFSLRWTCQPVRDGRGCCAGWVCSGTGEGDGASPDASLQAEILLRFEKNMEQ
                     LAILNDRIRNPLQVIAAFAEFADDDIRDKILLQVKAINETVRQLDKGWLESATIREYL
                     RKHHRMAL"
     gene            complement(28954..29838)
                     /locus_tag="PLV90_00415"
     CDS             complement(28954..29838)
                     /locus_tag="PLV90_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOF95603.1"
                     /translation="MSPQRSAGRPAGGLISPAANTWCPGCGNFAIEHMMKSAIAELSE
                     EEGIPLHNFVLLGGIGCHGKLVDYLNVNGFYGIHGRSIPAATGMRLANPDLKMICHVG
                     DGDIYAEGLDHLIFAAKRNSDITVIVHDNRVYGLTTGQYTPTSPFGFRGRSTPGGISE
                     HPINPLELMLAAGATHIARGYTKKVRHLKDLFKEAILHRGFSFVDVLQICATYFNLTD
                     YYNEHTYEMQEGDVETGRFESALDKIREWDYDRDAPIALGTFYSVRRPIYEEKFQALT
                     AGRPDRRALVRKVIERSR"
     gene            complement(29835..31493)
                     /locus_tag="PLV90_00420"
     CDS             complement(29835..31493)
                     /locus_tag="PLV90_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOF95604.1"
                     /translation="MREYSVLIGGKAGEGINTAGLSIARLFSRLGYRTYMYFDYPSLI
                     RGGHNFAIIRASARPIGAHRTPVDVLLAFDQNSIENHRQRIHRGTTVIYDASQVTKGE
                     GYGLPIDTIVREEDAPPITGNSAMLGALARVAGIGRGVLGDVLRESVPAKHLEANLRV
                     AGRGYDGVESAFSVEPLDAPTLPIMTGNEAAGLGLVHGGLESYVAYPMTPTSNLLHFL
                     AGQAEDLSIKVLHPENEISVILMALGLAYAGEKAAVGTSGGGFCLMTEGLSFAGMAEI
                     PVTIVMGQRPGPSTGMPTYTAQTDLHFVLNAGQGEFPRFVVAPGDIEEAYTWSSTALM
                     LSWKYQVPSIILTDKTLAEGGYSFDIEKIALHPDLEPVLWDGAGEYRRYARTDDGVSP
                     LAFPGREGAVVRANSYAHDEVGFTTEEAGAVTALQEKRFAKGESLKAELAAYPTVKIY
                     GPRNAGATVVCWGSEKGACIEAAERFGARVVQPLVFAPFPARAWKEAMIGAGKVVCVE
                     NNATGQLARLLRQEGFDPGRPVLKYDGRPFAVDELEARLREVLA"
     gene            complement(31668..32414)
                     /locus_tag="PLV90_00425"
     CDS             complement(31668..32414)
                     /locus_tag="PLV90_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95605.1"
                     /translation="MPLTLRTWEVRLGIVLVASSAAIYTVKYLLLGDPENTYTYIFNA
                     LGFLPINVLLVTLILNQLLSIRAKRERLEKLNMVIGTFFSEAGTWLLAFISDRDSELP
                     ELREGLIVTEAWTPETFAGVCDRLQHHHARIAVDAADLQDLRRYLQGRRDFFLRLLEN
                     PALLEHESFTDLLRAVFHLTEELERRDDFAGLPASDIRHLTVDIERVYRLLAREWLAY
                     MEYLQKNYPYLFSLAMRSNPFDETASPVVR"
     gene            complement(32470..33603)
                     /locus_tag="PLV90_00430"
     CDS             complement(32470..33603)
                     /locus_tag="PLV90_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOF95606.1"
                     /translation="MHTDTSPTLLVTGGAGFIGSHIATELLQNGYHIRILDNLTPRMH
                     GPDRREPGHLDRRAEVVVGDIRDPHRLREALDGIDTVIHLAAVVGERSSMYRIEKYLS
                     VNTSGTAVLLEALLDHPIRRLVVASSSAIYGEGLYCSYNGTVYPKVRRFPGWAAKGDW
                     EPRSPDGEVIYPLPTPETKEASPSSVYAVSKYDQEEMCRMIGETYGIPTTILRLFPVY
                     GPHQGYANPYSGMLTEYAFRLLQGLPVRLFEDGYQQRDFVSVYDAARAFRLALESPRA
                     AGETLNIASGRPHTFWTVASRLMAITGRQDLSPDITGTGRVGDIRHCTADISRAREVL
                     GYEPEVSLEAGLLDLAAWVRAEIAREEKVPVPLKVPAPSRLRV"
     gene            complement(33768..35357)
                     /locus_tag="PLV90_00435"
     CDS             complement(33768..35357)
                     /locus_tag="PLV90_00435"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HOF95607.1"
                     /translation="MNLRRPNANEATGTSNRSRDVVPASGICTRCIDGCKGACEIWLS
                     SFRGREALYPGPFGEITAGADKNYPVDYSHLNIQGYAVGAKGLPEGIEAGPDAAVFPA
                     VDTRTEYGWSAKVPMRVPIFTGALGSTESARANWEQFAIGAAISGITLVCGENVCGVD
                     PGLVLDHAGRVARSPEMDRRIEIYRRFHDRYGELLVQLNVEDTRLGTAEYVSAKHNLE
                     TIELKWGQGAKCIGGEIRVGDIDQANRLKDRGYIVLPDPGLHEVQKAFEAGALREFER
                     HSRVGFVTREGFLDEVDRLRDIGFKRVTLKTGAYSAVELAMAIRYGAEAEIDLLTIDG
                     APGGTGMSPWPMMNEWGIPTFYIESLACRFCERLRERGIRVPDIAIAGGFADEANVFK
                     ALAMGSPYVRAVCMGRALMIPGMVGKNIATWIEAGELPKTVSKYGRTKEEIFVCYEEL
                     RERFPDRIGEIPLGAVGIYTYTQKFRTGLQQIMAGTRNFSLGTVARSDLMALTEEAEA
                     VSGIPYVMRAYSDAAEAILDS"
     gene            35508..35978
                     /locus_tag="PLV90_00440"
     CDS             35508..35978
                     /locus_tag="PLV90_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95608.1"
                     /translation="MNFRLLPALIVLLLAFSLGAACTGSGGEAGPSTPGTGTPAATAT
                     PAPTTEVSLVPGPTQTIPPGKEVTFQITPGYPSPFTHDLTITFNGGKGQDYVNGIALR
                     VTKSNGEVVEKTLQPRIGDAVTINEAEGDNRVEITVSLVTGGSYKVTDEVVKVP"
     gene            complement(36013..36708)
                     /locus_tag="PLV90_00445"
     CDS             complement(36013..36708)
                     /locus_tag="PLV90_00445"
                     /EC_number="1.1.1.302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone
                     5'-phosphate reductase"
                     /protein_id="HOF95609.1"
                     /translation="MRPFVFVNVAMSADGKISTRERRQVRISGSTDFARVDRIKAESD
                     AVMVGIGTVLADNPSLTVKSPELRAGRRAAGRDEHPIRVVVDSAGRTPPDADILHKGA
                     GKRIVAVSRRADMERVAALGKHAVVVIAGDETVDLAALLAELYRQGVRRLMVEGGGTL
                     IWALFAQGLVDELQTFVGNIVIGGKDAPTPADGDGFTREVDFTRLRIIEIERLDDGLL
                     VRWSVEKESAARD"
     gene            36987..38705
                     /locus_tag="PLV90_00450"
     CDS             36987..38705
                     /locus_tag="PLV90_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOF95610.1"
                     /translation="MIVILSFSAVSFMLTVIAPDYGIYPANSLIYCIPILLAALWYPG
                     QGFRVTALLVAGFALIRIYFLTLGFAIDPVMTGLNMLFFFWVFGATVLFSQDPYLTIS
                     RCRQVVENTRDARLLCDPETLRVLCVSRRCADILGYAPPELIGIPVEKFWPDESERAR
                     FIAEMKREGYIGNMEATFRTRNGDARAVLISCRSLADENLVLCTVVDIGGHAALVQEN
                     GRLMELIHQSNDIFFMQDVAGRILHFSWLRAPEYGISPEGLIGQGVDALLSADLAREH
                     AARLQDVVEGKKSTCYDISLELAGTRHVFSITMAPYYGVDGTLIGVAGSARDITEVWR
                     QRLACRQMALEIDQWKGLVATLSHELRTPLQPLIGHLQMIVEDPGYYGLSEEAKGPLA
                     TCLACARQEQAAVEKMVGFGLLAADSVELVIQSVPLRSLVDSIVSSGGYDREAEFCND
                     IPETASLRGDRDRLYEVIETLVSNAVKYNEPPKKVWASYTESNRNHYITVCDNGIGIS
                     ADAVESIFGPFSTGGTEQPNRERRQTDPGLSIANKYVRLHGGDITVRSVVGEGSTFTI
                     RIPKEV"
     gene            38725..39135
                     /locus_tag="PLV90_00455"
     CDS             38725..39135
                     /locus_tag="PLV90_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOF95611.1"
                     /translation="MVVDDDLPTLEVMELLLKKIDREPLLVHNGWDALRMLKKEKPAL
                     IILDVMMSPIDGWQFLEELKRNEEFKAIPVLLFTAKHVWPEEYSRYAGDIVGVLEKPI
                     SLAELKTALERALPGSAASHRDNDTHARTQVRSG"
     gene            39250..40575
                     /locus_tag="PLV90_00460"
     CDS             39250..40575
                     /locus_tag="PLV90_00460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOF95612.1"
                     /translation="MIVTDLPCCPSLKEYRQIVGAEQFSAIEELAGRLSGIRMQEINS
                     TRQGGGVAEILASYVPFLNALGLETTWSVMEAKPAFFDVTKTLHNFLQGRDGFSPSMI
                     ETYWEAQRQNEGLIEDDADVVTIHDPQPLGLIEFLTGREREQKRLLWRCHVQLETVPT
                     GTAGSIGNILRRLVEKYHASIFSSFQYLPLWRVPGFIIPPFIDPLSEKNRDLPTAEVD
                     AVLAKYGIDAEKPIVTQVSRYDVFKDPVGVIEAFRKVRRKTPCQLVLVGGRACDDPEC
                     FLVLREVRETAEDDPDIHVLDLPPDSHREINALQRASDVIVQKSLKEGFGLTVTEGLW
                     KGKPVVAGNVGGIPLQIRDGWNGYLVSTVQETADRVLYLLRHPDAAGEMGIRGREFVR
                     EYYLLPRGVQDHLAVVDQVVNGKITARESVICYHPQVVTGRMAACAPRD"
     gene            40637..41080
                     /locus_tag="PLV90_00465"
     CDS             40637..41080
                     /locus_tag="PLV90_00465"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOF95613.1"
                     /translation="MKIAIASAGAGGLDDTVSPVFWASSSFTLIDAENQEILAVEIVV
                     APEGCAPGAAAGRFARDLAGRGVDAVIAGEFLPEETGVFSSAGIGMYRLQDMPIRDAA
                     KQVLNLAADTLRSPGRGAVPTDYVTRTYGGGVGLGSRRRTGGGPR"
     gene            complement(41087..41485)
                     /locus_tag="PLV90_00470"
     CDS             complement(41087..41485)
                     /locus_tag="PLV90_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOF95614.1"
                     /translation="MTGSEDEPSGTGRRETDSLPATARDYSILSAGFRVDILDRGDRV
                     IVIAELPGADEETIAIRLPNPQTLRITARRAGPAGEGAGDGTLSRLIRLPASVTAEGA
                     ATGYRNGVLEVSLKKVEGGSGPGGREIPIG"
     gene            41804..43048
                     /locus_tag="PLV90_00475"
     CDS             41804..43048
                     /locus_tag="PLV90_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95615.1"
                     /translation="MAQFKPFDMEGMPLEEQPMTWKDMSTAPYDKKAVDAYTRTRVIL
                     MNGIENNAVLMSHAVARMHPEPEVKKAMAMIRRIDSQHQVAINWLNPADQSVIETTLG
                     YEQVAVDLTANLAKNEPDPYVKQTLDFALLEDFDHLYRYSCLYDYIEGGDAEAIVQGK
                     TDVKPGRPTSIEHRHPDDSMRKHYDKDTADIKTKMNYVTIVAGEQQTMLYYRAHGSMY
                     PDDIARRLYAEIAEIEEQHVSQYELLGDPRETMLEKLALVQLNEAYLYYSYAQHESDP
                     RIKGIWETHMKMEIAHFNECARLIRKFEGRDIHDILKADVIEPLIVFESNKDYVDRVL
                     DAQLDLMPNNREYVRLRDLPDDWASFGYQAKVNAKGAPSEEIVSKAGRELAQRDQAEK
                     IKKIKQEMARKMEKGMAAVPAR"
     gene            43282..43521
                     /locus_tag="PLV90_00480"
     CDS             43282..43521
                     /locus_tag="PLV90_00480"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOF95616.1"
                     /translation="MPGFDGTGPRGMGPMTGRRMGRCALPPQTENSPAGEAGEGAPET
                     PVQQPQVYGLGRGGVPWGCGRGRGFGGGGRRRCRW"
     gene            complement(43678..44385)
                     /locus_tag="PLV90_00485"
     CDS             complement(43678..44385)
                     /locus_tag="PLV90_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOF95617.1"
                     /translation="MRIKFCGTASLTDMQCAVDAGCDAVGFIMGVTYRSADVVTPAEA
                     ADMIRHLPPFIEPVAVTHLQETTDLVRLAKDSHCTTLQVQNTVEPSELSAIRGALPYV
                     KIVKAVHVTDESAIATAELYEPYADALILDSRTEERIGGTGIPHDWNISAKIVANSTI
                     PVILAGGLTPENVREAVRKVRPYGVDVHTGIKKDGLRNPEKTLAFAREAQNALPNSGN
                     KRSAGKIRSGSEFARIR"
     gene            44898..45035
                     /locus_tag="PLV90_00490"
     CDS             44898..45035
                     /locus_tag="PLV90_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95618.1"
                     /translation="MFVQSVAPIYPSCIKKRVKGLIKTCTLPGLLDRLDIIEWFEYPG
                     G"
     gene            complement(45123..45416)
                     /locus_tag="PLV90_00495"
     CDS             complement(45123..45416)
                     /locus_tag="PLV90_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95619.1"
                     /translation="MGVAYNTVRKYVLRAQAVRDGAIEEIVPRDRKIHRPARVVTPEI
                     RGLIHRILEANRRKPKKQRCNAREIWQHLVASGYSLSHSTVKREVARWHAEQG"
     gene            45685..47301
                     /locus_tag="PLV90_00500"
     CDS             45685..47301
                     /locus_tag="PLV90_00500"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HOF95620.1"
                     /translation="MVSEEVIPESFTKCLARLGIIAALVEGFGIVDLVNARLPRLQHR
                     TLTHGDVVLALILNGLGFTQRWLYLFSAWFENKPLERLFGKEVTAADITDDVIGRTLD
                     AIEEYGPERLYQEIISQIFLVTRLGYHQVHLDITSFNFYGASDTTSTVHVAQGYPEDG
                     RWGLNRSGLGLIVNQAGVPLSMRFLSDNGDDKVALPEMLARFRSRVEFPEPLYYIADP
                     ASYVGDSFKKIGEETFFITLVPGTTGEQQALLRRDLPLAPMADPRYSFFETESCYGGV
                     HQKWVVYHSAADHAKQEKALEKRRSKEDERVKKALAHLNNREFACEADALAEAERWIR
                     TYPLYEFESVSVVTRWQQAPGRRGRPEKDGVPGAVYLLAASVRPDEAARVAERAALGR
                     FVLATNDLDLAAEDLLARYGNRGVVEHGFRFLNDSSFTISDLFLENNGRIEALGMMMT
                     LMLAVYSLGEYQLRRRLEHLGKTVPDRAGKPTAKPTLKWVMTFFDGVVEYHRYDLATG
                     TVQYEGILNMSKRCWDVVEAFGPICKKYYS"
BASE COUNT         8491 a        14879 c        15235 g         8982 t
ORIGIN      
        1 tgaaaaatta tgtgaccacg taaggtcggc agcctaaaag tatgggaatg tggggggatc
       61 tcccgctgta tcttgagaat ctccgcgacg ccgcagttgc catccgttct tatgtcgggg
      121 gcccggatgt ttgaggagtt catgggcgat cacatggttg cggggcctat gaattaatga
      181 actcggtcaa tagccctgcc gaatatccgg ccagttcatt ggtttattgt aatattgctc
      241 cttacattcc tccgacatgt tgtagaaaaa ttcaggatag acttgttcca tgtattcccc
      301 gagggagatg ttctgtttcc ataacttcgc cgctatttca gccctcttct cgtcctgagg
      361 atcgggaatg ggaggtgact tcatgccgca atcgtcctca ctgttgtaat atgcatgata
      421 atatgcctgt aacagtaaac caaacaccga tatgacgagg atcagcgcga gagttaaaaa
      481 aataattttt tttgaaattg tcatggtgcc agcctcattt cggaaacctc acggtaccca
      541 gatctcgtta ctgagagtat atagtcactc ccctaacaat ttagaagcag actttgggct
      601 caagaattta agcacccatt tctcccaata acttctcttc gccactaact tcttaaacgc
      661 ctctgcgatc gtggccatca ggtgctcacg gccggtgatg aacgtcgctg ctacctctcg
      721 tttgatcgcc cgccatacct cctacattcc cggaactcat aacgagatgt acgcaaactg
      781 gccttatttc aattaaaatc cgaaatagag tgaattttgt ggatttgcac aaacaggctc
      841 gttatgtgta acacataacg agatgaaaat tgattgcttt gatttcgaag gatgattggg
      901 atttaggggc aataatggcg cgggtcgatt ggaatcgtta cgtattgtcg ggaatccagg
      961 tttcaccccg gaggttcgtc ccgaatggct gacacggggt gcactctcgt gcgtactgga
     1021 tcggttcttt gccgccgggt gtgaacacct cttcacttct cttgccctcc gggtctacac
     1081 cgcgttcgat atcccgtttt atcccattct ccacggtgat accacctcga tctccctgta
     1141 tagcacctac gaaggggctg aagactctcc cccttccgct cccaccatct gccgcggaca
     1201 cagcaaagag aaacggggag atcttgtcca gatcatgctt ggtcaggtct gcgactccca
     1261 cgggatcccg ctcatcacca ccgtccatga tgggaacgag gcagattgta cctggaacgc
     1321 agacgttatc cagacccttg ccgaactgga tccgttttcc cgtactgatg tcacctacat
     1381 cgccgatgcg aaactggcaa ccgctcccaa cagccgaaaa ctcctcaaga aaggtttcca
     1441 gttcgtcacc cgctgcccgg caaacttcag caagaagatg gctgcccggg tgacccgggc
     1501 agcctacgag gcagatgcct ggctacctat cgggtcatat cgggataccg ataaggatac
     1561 gctggagacc tacgacatgc aggagttcac ccaaacaacc gatgacagaa tatcctgccg
     1621 tttcctcgtt ttccgaggca gccaccgaag agatgaattc gaggctgatc tggtaacgaa
     1681 acgggaggag ttcgttgcaa tcctcaagga aatgacccgg aaacggtttg cctgtgagcc
     1741 tgatgcgcaa caggccctcg aagaggcacg taagaacctc cgcaggtatc cactctggaa
     1801 cgctgatctg actgttgaga ccgtgatcac cgagaagaat ccacggggac ggccagggaa
     1861 gaatccccgg ccaacgatcc aggtcaccgc gtgggtgatc cgggcaagcg aaccgcagtt
     1921 gcagcagaag gtctatctgg ctgaacggcg caaggccgag tcgtttgtct tgatgacaaa
     1981 cgtttcggag gaggatacac cagcacgtga ggttctccgc ctgtataagg ggcagaagac
     2041 ggtcgaggat aacttttcgg tcatcagacg accgacggtg gtcgataccg tttacctgaa
     2101 gaaacccgag cgggttgcgg cgctcgtgac actgctggcg gtagctctcc tgatccaggc
     2161 agtgatccgg gtgttggtgc gccgcaacct ggacgctctg ccagaaccgc caggactcga
     2221 tcatgggtgt aaaccgctca ttcgaccagg attaaagaag attctccggt tcatcggcta
     2281 ccattcgctc atcaccaccg gcgatgaacg tcggttctgg tgtatctcgc cggatcatga
     2341 gaaaaatctg ggaatctggt tgcgcttgct tgagttcaat gtagatgggt aacatccgcg
     2401 ctcttgacgt tctcctgccg gaaaggtggc gagcggaagc gctcgaggaa acagggttct
     2461 aaagcggttt ggatggctat gggggagggt attgagctac agtacttcag gagttaccct
     2521 gggacaggga gagaaccaac acctcgacca tggtttggga gatgagagag gaaaatgtta
     2581 gaggggatcg gacaaggtct tgcaaagtga gatgaatgta aattagtccc ggcaaatagt
     2641 aagtgatcgt gggagcgcga taattagtcc ttatggcgct cgagaccaag cagtagataa
     2701 ccgacgacga gtgctttgcg atgatcatca gcgtcatggg cgccaccggg gggctcatgc
     2761 cggttcggga tgagcgcatc ccggagggct tgcccatgag cgccggttac acacccggcg
     2821 tgttcatcgg caccgacgac ggattcaagg cggtcgtcgg tggcaagggc ggcggaacgg
     2881 caagggttgc ccaggatctt gtcgagggtc tgaccgaggc gcaggccgtg gccctggcaa
     2941 agaaagtgat cgcgttctac cgggagaacg ggcaggcccc cgaacgcctg ggagcgacga
     3001 tcgaccgggt cggcttcgac aaattcaaga acgctgtcct ctaaacccct ggaaaaggtt
     3061 ttacgggaga tcgatgcatc cgcatgccgg cggacggcac taactcccac cccatttatc
     3121 tctcctctgt tctcacgaag tggtggcgag ccgggtttat ctccggcgca gcccgccggg
     3181 gatcatcctg cataccacgg catacacttc ccgagaccac cccgggagaa aaaaagaata
     3241 tcagccgata tagttcagtt cttcccggcc ccgggcttcc tcggtttccc ggatatttgc
     3301 tatgatctgc tccatggcct cggcacgctg ctgcagggtg gtgtggtcga tatcgatccc
     3361 gatcatggcg gagaggacgg tgaggagact gtcggcgctc ctcggatcga cgatgtaacc
     3421 gctcgtctcg cccatcaggc agatgccctc gatgccccgc gccttcccga ggccgagcag
     3481 gaggcccgat gcccccatga tcatgccgcc gctcccggcg ttctcaaacg atcccccggc
     3541 cgcctccacc tccggccgga gacgctccat attgatcgcg gagagcaccc gcgggtgctc
     3601 gaccaggtgg ccgacgccgt atcccccgag cgtgtagatc ctccggacgc caagagcctc
     3661 ggcgaggtcc aggtaccgct cggccaggac gtagtgcccc tcggccgagg tgctctggaa
     3721 gtcgcccacg aggaagagga tcgccacccc atccttctcg cagcgataga tctcgttgtt
     3781 gaccaggtgg gtgacgccct gttcgtcgac gaccacctgc ggcgggaagt ggaccgagga
     3841 tatctccgct atcttctcgc cgccgagttc atcgatgagg tggtcggcta caagtttccc
     3901 gacgtgcccg atgccgggca ggccctcgac caggaccgga gcgccgccgg cgtcatcgcg
     3961 gaaaaaggtc acgacgacgt cttccatcgg cgcatcaccc tcctgtagtc cccatgccta
     4021 tcctcgtgag aaaagcgtgc cggatgagcc ggtcgcgtcg accggccgca tatcgggcag
     4081 acctgcgcaa gcgtatacct caggtcgtcc ggacagcggc gaatgcgatt gctcatggcg
     4141 cgttatccgg ccttctgctt ccggacgaac ttgccggaac cgcccgcccg ctcgaccacg
     4201 ccgacggcgg cgctcgccgc cttctcgatc gcccgctcgg ccgccttgta atcgggagcg
     4261 gtcactttta tccggtattt cggtgctccg atgtagatca ggtcgatatc cacatcctcg
     4321 atcttcggct gcgcgctcct gagcgcacgg cggataacgt tgacgccgtc gggccgcggc
     4381 gacgtcagga cgagatgccc cgtgatggtc accttcgaga ccttcacgtt ctcgtttgcg
     4441 atcgtgatga gcgcttcttt gaccggttcg tcgaggccca gcctctcggc cgcctcgccg
     4501 ccggtggtga cgatatcctc gagggccgga tagagaaggc cgtattcgcg gtatatcgac
     4561 tcttcgatga cctttcggtc cacacccgcg gactcggagg caaaccctat ccacttctcg
     4621 gccttctgct cgttcttcca ctcgtggatc ttctcgcggc gctggtgctc gttgacatcc
     4681 tttaacgaga gatcgatgtg gccgcggccg gggtcgacac ccaggacttt gcagacgacc
     4741 ttctgtcctt cccgtatgta gtcccggata tactttatcc agccccgcgc tatctcggat
     4801 atcggtatga gccctcttcg cccgtcgtac tcgtccaggg tcacgaacgc cgcaaaatcc
     4861 ttgacatccg cgaccgtgca gacaacgagc tctccttcct cgggccactc tctctcgtgc
     4921 atggaataat cactcaagta ctgccagtat atcagccttt atatctgcct ttccgccgtg
     4981 gggctcggcg aggatacgcc cgcagacgct gcacgccacg acggtgcttg ctctctcaaa
     5041 gactatctgt tcgttctcac agtcggggca cttcacccgg aggaacgtgc tcctgttctc
     5101 tctattcaac cggaccatta ttaatctcac tccgtaagct cgaatttacc gatccggaat
     5161 cccggccgga ggtgcgcctt cccgcagacg gtgcagcgat atttcacgtt gatcttcttc
     5221 gtcggcttgt cgccgccggg caccttgctg aacttgccca tgttgcccac catacttcta
     5281 cgatctttct ggcggtcgat ccagttgaag tggcgtactt tgcccctctt caccttcacg
     5341 acctcatgag tctggtgatt ccggcagaat ggacagtacg tcttgaattt tgatggcatt
     5401 ttcatgatag cagcctctat tcaataggtt cgagtaccta tattacttgt taagcctaat
     5461 atttaaggct atgtcgtgtt cgcagagcac gtctgcatta ttctccggca gggtgacgat
     5521 atccccctgc gttaagacgt agaccctgcc gtccactccc atgaacgatt ccacatcgga
     5581 gcggatatgc acgagggcat acggagacga gagcgggcgg ggaattgtcg gggccggcct
     5641 ccctgctccg gcggcgggag ggatatccgg ctcctcgatc gcgtccacgg ccgccgcacc
     5701 gccgccctgg aatgaacctt cctccccgcg gatgagcgtc tcgcggcaac cctcgacggc
     5761 gtcgatgatc tgcccgtaca tcgcccgttc tgcggggagc atcttccttg cctcccccct
     5821 gtcgaagtac tgcccctcga tctgctggaa agcaagatcg agtatccgcc gggtccggat
     5881 ggcaaatatc tcctgcacgg tctcggtgat gctcccgatc tcctcgatga gcaggcttcc
     5941 cgactggctc tcgagggggt tagggagacc gtagtagtcg gccttgagct tctcgacgta
     6001 tgcccgggca ccctcgtaga gagcgggctc gatatcggag agcctgcccg agtggtgcat
     6061 ctcaaggagc gtctggcgca gcctctccaa ggggtcggcg gccatggttc aggcgccccc
     6121 ggcaatctcc gccatgctcc ggcagcagag gaagaccgca agcgcttcgg gaacgcttgc
     6181 aggaccggtt ttgagcgtat acgtgttccc gagcagggat gtggcgacgt tcatctgcag
     6241 atccacccgc acctcgcggt ccccgaatac caccgcatcc acgagcgggt cgaactccgc
     6301 gaggtcccgg gaatcgagcg gcaccacccg catgccgctg ccgccgtgca gcgacccggg
     6361 cgcccggatc agccgcttga tatcggtcgt gaccggttcg tccgcacgcg ccccggcgcc
     6421 ccggacccgc tcctcgaacg cgggggtgcc catgatcgcc cggacgatcc ggttctcccg
     6481 gagggtgccg gggtccttct cccggagggc gccaaggcct gcaagaaact ccgcagccgc
     6541 ccgtttcccg atacccgccg gtcccgtgag gtatgccgcc gcctccgccg catcgagccc
     6601 ctcgagccag aggaggtggt cgcggagggc ctcgatgtag cgccaccgcc acccggccgg
     6661 ggttctcccg ggagcaagca tcacccccgg gtcaagcccg atgccgcaga cgtaatcgac
     6721 gacctcgcgc cgctccgggc tctcccatcc cctgacggcg atatcggtga cgtggatatg
     6781 gtagccgcgc cccccggaga aggcgatccg gatgtggtta cgcgcaaaac cgagctcgtc
     6841 ggtcagcatc ccgaccagtt tcacggtctc ctccttgacc cgggcaagca tcatcgcgta
     6901 ggggccgcgg acgatctggt cggcgtcgag gtcgaagatc aggtcggcgc cggcccagtg
     6961 cttgtcgttc atcgtcggcg ccgacggcgt ctggtagtag gccgtcgaat agtaggcgtg
     7021 ggcggggatc aggctcctga cgtaagcggt aagttccagc gggtcggcaa acgccatatg
     7081 ccgccgcatc cggacggcgg acgcggcatc gaagaagaca aaaccccatt cgcgctgctc
     7141 gagagaggat ggagcggcga gggtctgcct ctggtagtac tcggcaaacc tctgcttcac
     7201 aaactcgatc gtcgcaggct tcatgagatc gtcttgatca tatatggccc cactcgttca
     7261 tatccctgtt tcctgtagta ggggcgcacc ccgacaccgc tcatcaccgc gagccgcccg
     7321 tagccgtggc cggaggcctc ctcctcggcc gcggcaagga gccgcacgcc gtaactgcgg
     7381 tgctgccact cgtcggccgc tgccggcgtg ccgaggggga ccacgctccc gtagacgtgg
     7441 agctcgcgca ggagcgccgc gccggcaagc tcgtcgcgga atggccggtg cgggaaccgg
     7501 agccgggcaa acccgacgag ggcatcctca gagccggcct ggatgaaccg ctcctccccg
     7561 ccgcaggacg ggtaggtgac ggtcgatagc gccgtctctt ccggcgcaac cgacctcccg
     7621 acctcgcggc accggatgca ccggcatctg agcccctgcg agtggagccg cgccgccgcg
     7681 agctgccgga agttgctgtg ccgcgaaccc gcgacgatca actttgccgg gatatcccgc
     7741 tggatccgct ggaggcggac gtactccggg aggagcgact tcgcgtaggc cacgaggtcg
     7801 accagctcct cctcggtgta ggggcggtag tcgccccgct cccagagtgc ctcgatctcg
     7861 gaggcgggcg tcaccagggt cgggtagatc ttcagaaagt ccggccggaa ccgcggatcg
     7921 gcaaagagtt cgcgaaacat cctccggtcg tcctccatgc tcgccccggg gaggttcggc
     7981 atcacgtgga accccacctt caggccggcg tcgcgcagga ggcagtttgc cgcgaccgag
     8041 tccgcgacca ggtggccccg gcggttgtag tcgaggatcc ggtcgtcgat ctgctggacg
     8101 ccgagctcca ccttggtcac gcccatcgca agcatccggt cgatgtgttc cgcccggctc
     8161 cagtccggcc tcgtctcgaa cgtcgtcgcg atgcaccgga ccccggcgcg ctcgtttgcg
     8221 gcgaatatag cgtcgaggtc gggtttctcc ggcgggacgc cggcccgggg gtagtcgttc
     8281 atcgcctgca cggcggcccc gacgaaccac tcctggtact cgaccggccg ggcggtcatc
     8341 gtcccgccca tgacgatcag ctcggccttg tcgacatggt ggccgagcgc ctcgaactgc
     8401 ccgagacggg cctgcacctg gcggtaaggg tcgaacgcat gttcgcgggc ccggagcgcc
     8461 gccggctcct cgccggtgta gctctgggga gacccgaacg ggtgttccgg cccgccgggg
     8521 caggggaggc acttcccgtg cgggcagggg tgcggcgacg tcatcaccgc gacgggcgcc
     8581 acccccgaga gcgtccgggt gggtttgacg aggaggagcg gccggaggcg ttcgcgctca
     8641 tccggcgtgg ctgcggcgag gactgcggag ttcctgggca tgttcgcacc gtgctcccgg
     8701 cagacctcga gcttgatcct ctggatgtcg gcagggtcgg gcccggcaga aaggatgcgg
     8761 gagatgatct cccggagggt atcagtatcg ttcatcgctt aatgttcggt tgcaatgcgc
     8821 cccgatggaa aatcagcgtg ataatcgatg taaggattat ggttggcccg gatatgctgg
     8881 acgatctcgt ttccgagcgt cagaatggct tttttatgcg ttactttatt cttatggata
     8941 tggacgggtg agatgtggag agcattatac tggtcggtcg agacgtcctc ccccgtagtg
     9001 ctttcataat actttcgtac gtggactaac agcatgtgta gatggagcag ctcttctttt
     9061 tgcacaatat cacctttcct cgaagaatac tactgcccgc gggtagatat aatttgttgg
     9121 tgatcttttt atatgcacaa ggtgcgggca gtcggagcgt tcatcgggct tgcaatcggc
     9181 gatgcactgg gcgcgcccct ggaaggcttt ccgccgcctc cgttcaccgt aacggagatg
     9241 gaagggggcg gaatgcacca cacccttccc gggcagtaca ctgacgatac cctgcaggcg
     9301 tcagccctcg cggagaccct cgtccgatgc gggggtctgg atccgggcga cttctcccgt
     9361 cgcctgattt cggcttaccg ggctcgtccg gagttcttcg ggccgacatc ccgggccgtc
     9421 ctcgacctcg tcgagggggg cgtccgcccg gaggatgccg cgaggatggt gcacgatgcg
     9481 cggggaggga gccggagcaa cgggagtgtg atgcggggga taccggtcgg catcttctat
     9541 ccgccggatg tggtgcggga ggcgagcctc gcggcatcac gggtcacgca ctttgatgcc
     9601 gcagccggcg aggcctcggc gttcgtcaac cggatggtca gcgggatgtg ccggggagaa
     9661 gaagcgagcg cggcgttcgg atacgccctc gccgcctgcc gcgacccgga actccacggc
     9721 ctcctccggg actggcgagc gcacccgcgc gaaccctcgc tcgatgccgt cctctgcacc
     9781 cactgcgccg tcagcgtctt catggatgca ccgaccttcc gcgatgcggt gatcacggcg
     9841 gtcaacctcg ggggcgacgc cgataccgtc ggcgcgatcg ccggcggcct tgccggagcg
     9901 tgctgggggc ttcacgcgat accgggctcc tggctctccg cgctccagga ccgcgagatg
     9961 ctcctcgacc ttgcccggcg gctctcacag gcaggcaggc cgtagtgcac gatatcgccc
    10021 cgggaagttc cggtgcatgc cgggatgggt actctggctt ctcgcgagag gccgtatcgc
    10081 catccgcacc ggtcacctcg cgcgaagact tgcccgggtg cctgcaactg tagcgtcaga
    10141 tgggccgcag ttccacgagt ttcaggggcc ggcccctctc gcagacgtcg ggggcgttgc
    10201 cgaggacctc cccgacgata tacttctccc cctcgatgat gccgtcgggg cggcagagcc
    10261 ggtagctccg gcacgtggtc cgcgggcagg agaactcgta actgattttg gagttcgcga
    10321 tcgccatatc ggcgcctatc agcgcgacga tgggagcctc cagcacgtcg accgcgacca
    10381 ccgcctcgtg gtggacggga cagtcctgcc gggtgttgga gcggatggca gcgacccggt
    10441 acttcttccc gggctgcagg ttgtggcagg tcttacgcac cttgcagctc tcgcactcgg
    10501 tgcacacgtt gccttcgtag acgaaatcga gccccggttt tgcaagcgcg gcgccgatca
    10561 gggtcacttt tgtctttggt ttcaccatac tttcgctcac tcccggtaca gcatcttcac
    10621 gaggtcccgc gccgatgccg cggtcagccc catatcgagg atcgtgaacc gctccggccg
    10681 gatggtccgg gcggcaagca gcgcctccac ggccgtctca tcgtcgatcc cgatctcggc
    10741 cggggtggtc ggcgccccca ccttcttcaa cgagtcgcgg atcccctccc agtccccgcc
    10801 gtggagatac atcgttatga tggcgccgat accgcacttc tccccgtgga ggcccttccc
    10861 cggcgcgagc cggtcgaggg cgtgggagaa cttgtgctcg ccgccgctgg ccggccgtga
    10921 cgagcccgcg atgctcatcg cgacccccga agagaccagc gctttcgtca cgatccaggc
    10981 gctctcttcg gagtgcggtt tgatgagatc ggcgttcttg aagaggatct cggcggtcat
    11041 ccgtgagagc gtcagcgcat actccgagag cgcttcgccc ctgaggcggt gggagagttc
    11101 ccagtcgagg atcgcggtgt ggtttgccat gatgtccgca caccctgacg caagcagccg
    11161 gtggggtgcc gacgcgatga tggcggtatc ggcgacgacg gcgatcggcg gttctgcggc
    11221 aagcgagacg ttcccgtcgg cggtcgggac cgaggccctc gatgaggcga tgccgtcgtg
    11281 cgatgcggcg gtggggacgc tgatgaagtg gcggtcggtg ttgtaggagg cgatctttgc
    11341 ggtatcgatg accctgccgc cgccgacgcc tatgacgaaa ccggcctttg ccgccgacgc
    11401 ctcggccctc ttgatcgtct cgacgggatt gtttgggtcg gctgcacacg tcgcgacgtc
    11461 gtaggagccg gcaagcagcg cctcgacccg tctcccggcc agatcccttg tccggtctcc
    11521 cgatatgatg agcacggagt cgccgagagc aaggtcctcg cagacggtcg ggagctgctc
    11581 gatgacgtcg tggccgatga cgacgtcgcg ggggagctgc atccatttgg acttatcgaa
    11641 gaccttggtt ctgagtaggt ttatgccgtc tgcgctcatt cagatcagta atgattaggg
    11701 agttcctcta caaatactac atcgatccca tccgttacgg tcagccgtat acgctcgtcg
    11761 atacgctgac ctacgccctg atcctcatcg cagcggtcta tatcgtctac cgggggctcc
    11821 ggcggtataa gattgccgtc gacgacgaac tggtgcttgc aaccatgccc ttcgtcgtcc
    11881 tcggcgggct tctccgggtc gtcgaggata cgggcatgat cgcgtcggac ttccggtttc
    11941 tcctgatcac gcccctgatc ttctttacga tctttgccgt cgcggcggtc tccctctttc
    12001 ttgggaagct cgcggagaac gcgggcttcg tcgcccgcta cagccgggtc tacgggggag
    12061 cgggcatcgc cgcctgcatc gtggccggtg cggccctcgt ctggttcggc ctcaccgaaa
    12121 cgaagatcgc gctcgacgtc cttgccgcca tactcgcgct cgcgtcggtc tcgtcgctcg
    12181 ccctctgggc cctcctcgcc tacggcgcaa aatgggacta cgcctcgaac atattttaca
    12241 aactcctcat attcggccac atgctggacg caagcgcgac gagttacggc atcgagatcc
    12301 atcccatcca ttacgtcgag cagcacgtcg tgggctcggg cctgatcgag gcgaccggca
    12361 ccgcattctc tatgttcctc ctcaagatcg tggtgctcat acctgccatc tacatactcg
    12421 aacagtaccg gcgggaggga aatcccggtc tctggcatct catactgctc gccatgatca
    12481 ccgtcggcat ggcgccgggg gtacgggata tggtacggat ggtactctat gtttgaacgc
    12541 ccgatcgtcg gggccacgat cgttgcggcc gccctctttc tcgtctcggt cggcctcggc
    12601 atttttgccg tgatccagga cccgtcgacc ggcgcacagg tcatgaccct ctttcgcgat
    12661 caggtcgtag atcagctcct ctccgactcc ccggcggtcc tcgcgggcaa gatcttcgta
    12721 aacaacctca gcacctgcct cctcctcttc ctcggcggcg catccctcgg catcgtcacc
    12781 ctgattgtcc ttacggtgaa cgggctcctt atcggtgcgg tggcggaact cgtgcggcag
    12841 cagcagggcc tcctcttcat cgcggcggcg ctcgccccgc acggcatatt cgagataccc
    12901 tctttcctca tcgccggggg cctcgggttc ctcctcgggc aggaactcat cgccgagtgg
    12961 cacgggagag gcgatgccgc cgccggcgcc ctgcccctgg cccgcctctt tctcagggta
    13021 gtcgtcccgc tcctcgcggt tgccgcgatt gtggaggcat ttattacgcc ggcagtccta
    13081 agtgttatca cataaagcag gcagatcgac gctttttgag gtacgagacg gatggaagaa
    13141 gatactggcg cactcccccg gaaagacgat ttttccgagt ggtataacga ggttctctgg
    13201 cgagccgaga tcatggatgt ccgctacccg gtcaaggggc tctacgtctg gtacccccac
    13261 gggtttgcca tcaggaagcg tgcatacggc atcctccggg acctgatgga ccgcgaccac
    13321 gcggagacga tgttccccct cctcatcccc gagaccgagt tcatgaagga ggccacgcac
    13381 atcaaggggt tcgaggacga ggtctactgg gtcacccacg gcggcaggaa cgagctcgat
    13441 gtgccgctcg ccctccggcc cacgagcgaa accgccatct accctatgta ctcgctctgg
    13501 atccggtctc acacggacct cccgctcaaa ctctaccaga tcgtcaacac gttccgctac
    13561 gagacgaaac acacccgccc cctcatccgc ctccgtgaga tcacgtcgtt taaagaggcg
    13621 cacaccgtgc acgcgacgcg cgaggaggcg gaagcgcagg tcgagactgc gctcggcctc
    13681 taccgggagt tctacgagag cctccgggtg ccggtgatca tatcccgccg cccggcctgg
    13741 gacaagttcc ccggcgccga ctataccatc gcggtcgaca ccatcatgcc ggacggaaag
    13801 accctccaga tagggacggc gcacatgctc ggcgaccact tctcccggac ctacgagatc
    13861 acctacgagg acgcgaacgg ggagcggcag tatgccagcc agacctgcta cggcatatcc
    13921 gagcggtcga ttgcggccgt gataagcgtc cacggcgacg ataaggggct cgtgctgccg
    13981 ccggaggtcg cgccgatcga gatcgtcgtc gtgccgatca tcgtcgggaa gcggcgcgac
    14041 gaagtgcttc ttgcggcaag agcgctcgaa gaggagctcc gaagcgccgg gttcgtcgtg
    14101 aagctggacg accgcgacct gcgcccgggt gcgaagtact accactggga gatgcgcggc
    14161 gtcccgctgc gtctcgagat cgggccgcgc gacatcgacg cgaacacggt cgtcgccgtg
    14221 acccgcgacg gccataagat cacgctcgac cggcggggcg tcgtcgaagg tgtcttctcg
    14281 gcccttgcgg cgttcaggaa agggctggag cagacggcgc ggcaggcgat gatcgaccgg
    14341 atcaccgtgg cggcgacact ggaagagacc gtccttgcgg tgaggaaggg ggttgcggtc
    14401 gtccactggt gcgggtcaaa ggagtgtgcg gaagagatcg aggcggcggt ggatgcaagc
    14461 atcctcgggt ccgacatccg gtcggacctg atcaccgtct ctcgcggtcc gtgcatcgcc
    14521 tgcggcggag acggcacgtc caccctggtt gcccggacct actgatcagc aggacgggca
    14581 ggtatactcc cgggggtgct cgggggacga tatgtctccc gatatagccg cttttccccc
    14641 ggagaagaat atcctgccac atttcgagca tctcttcggg agcagacgct cccagcggct
    14701 catcggaact tccagcgtga actgcatctt ctccgcatcg gcggggttgc actgggcgag
    14761 ctcctcatac cgggagagga cctgcatgac ctggagcggg tttgctccct cgttgcagaa
    14821 tcgcctgaat ataaaaaatt gaaggatcat ccacccaagg tcggagcgct cgagggtctc
    14881 ctcgtactcg ctgcagaccc cttcgtcttc atcgagagag tacccgcaga ggggacagcg
    14941 cccgcccacg agttcgtcca ggaagacctc ttcatggcag ccgggacagg tggtgtgggg
    15001 ggtatgcatt ctggaagtca tggttgtgct ggcctcatac tttttactct tccagcggca
    15061 cgggagagag ccgcatgccg tttgaaagta ttccacgcat tatgcgcggt ctctacaatt
    15121 gccctgaggt tttactacgt attgcgctcg tggtgctctt atacgtgtcg aatgcggtta
    15181 tacggatcca ctctggcggc gcgtgccggc cgctgcggcg catcgctcgc ccacccgctc
    15241 tccggccgga gccgcaccct tctgccgccg cccgctcccg gcgatccgaa tcccgcgaaa
    15301 aggcaatatt ttccggatct gggctttttt ggggccgccc ggagtacatt tttatagatt
    15361 ggcggggcac tctgcgcgca tggcatcccc ggcggcatct gaagtctata tcatcgacgc
    15421 atcggatcgt gaccacgcca tcgaggcgct ctggcgcgag gtcggctttc cccccctcga
    15481 tggaaaggat atcgcggtca aggcgaactt caacagcgac gacccgttcc ctgcgacgac
    15541 gcatcccgat accctggggg ccatcttcgg ccagctcctc gatgccggcg cccggacgat
    15601 ccgcctcggg gaacggagcg gtatggggga gacccgtgcg gtcttgagga accggggggc
    15661 cgtcgacgtc gctgcccggg caggggcgga tgtggtggcg ctcgacgatc tcccccgtga
    15721 agggtgggag gcggttccgg gagacggcct tcactggaga gaggggttcc tgatcgcccg
    15781 gctcttttgg gatgccgatg tggtggtcca gacctgctgc ctgaagactc accgcttcgg
    15841 cgggcacgtc tcgctctcgc tgaagaaccc tgtcggggcc gtggcggaga agaaccccgg
    15901 gggcggctac aactacatga tggagctgca tgcatcgccg caccagcgcc ggatgatcgc
    15961 cgaggtcaac cggttcttcc cctgcgctct ctcggtcgtg gatgcggcgg aggggttctc
    16021 gaccgggggc cccgagcgcg gccaccgtat cgctcccggc gtcatactcg cgagcaccga
    16081 ccgggtcgcc ctcgacgccg ccggtgtcgc cctcctccgc ctgtacggtt cgacgccgga
    16141 tgtgatgcgg ggccgcatat ttgcaatgga ccagatcgcc cgggcggcgg agctcggcgt
    16201 cggtgtccgg tcggcgcagg acctcaggct cgtcgcgctc gatgcggaga gcaaggagac
    16261 cgccttcgac atgcggcgga tcctggacgg ggccggctaa agaccgagca ggatgcgcag
    16321 gccgatgccg atcaggatcg ccccgccgag gacctcgacc ttctgcccga aggcccggcc
    16381 aagcgtactc cctatcagga cgccggcagc ggagagggca aatgtcacga tgccgataac
    16441 gaccgcaggc cagaggaccg gcgtccccag aacggcaaac gtgacgccga cggcgaacgc
    16501 atcgatgctg gtcgcgaccg cgagcaggag aagggtcgct atgcccacgg tggcgagctg
    16561 cacggcctcg ccgtctcccc gcaccgctcc ggcgatcatc ctgccgccga ccagggcgag
    16621 cagcaggaag gctatccagc ggtcgtaggt gccgacgaag gaggcgaggc ctgccccgcc
    16681 gagccacccg agcaccggca tccccgcctg gaatccgcca aagagcgccc cgacgacgag
    16741 ggcgtacctg aaccgggcct cccgcacggt ggtgccgccg ctcgcggcaa cggcaaatgc
    16801 atccatggca aggccgacgg cgatgaggag ggttgtcgcg aactccatat ctcctgtaca
    16861 tgaaggtgcg atgccggata accgtatggc ccggcggttg ttataagacg cgggagtgcc
    16921 ctacgatacg gtgatacaga tgagcacaca gaagatcggc ctcatccaga cggcggtggg
    16981 ggacgacctc gaccgcaacc tggcccacgc ccttgccctg acgaaacagg cgatagcggc
    17041 gggtgcccgg gtcatctgcc tgcaggagct ctaccggacc ccctacttcc cgcagtacga
    17101 gggaagggac gcatctttct acgccgagac cataccggga gcctcgaccg atgcgttctc
    17161 ggcgcttgcc cgggagcacg gcgtcgtgat cgtcgtgccg ctctacgagc ggacggcgga
    17221 cggcgagttc tacaacacgg cggtcgtcgt cgacgcggac gggcacctcc tcccccccta
    17281 ccggaaggtg cacatcccct acgaccccct cttctacgag aagacctact tccggccggg
    17341 ggaccgctac cgggtctacg atacccgcta cggccggatt gccgttctca tctgttacga
    17401 ccaatggttc ccggaggccg cgagggctgt tgctctgcag ggcgcagaga tcgtattcta
    17461 cccgaccgcc atcggccgga ttgcgggcga ggaggagccg cccgagggcg actggcgcga
    17521 tgcctgggag acggtgcagc ggggccacgc gatagcaaac agcgtccacg tcgccgccgt
    17581 caaccgcgtg ggcagcgagg gcgatatccg gttcttcggg agctcgttcg tcgccgacgc
    17641 cttcggaaac gtccttgccc gggcggggga gaccgccgag gaggtcctcg tcgttgaggt
    17701 cgaccttgcc ggaaacgagg ccgtccggga ggggtggggg ttcttccgga accggcggcc
    17761 ggagacctac ggggcgatca cccggcggtt cctgccggca aagacaccgc aggcgctcgg
    17821 ctaccgcatg ccggcggagt gggagccgca cgatgccgtc tggctctcct ggccccacga
    17881 ccgcgagacg ttctccgacc ttgcagcggt ggagagagct tacgtcgcga tcatcgcggc
    17941 gctccgcggg tccgagaccg tcgacctgct cgtcacgagc gatatgatgc gcacccgaat
    18001 caaggcgatg ctcgaggagg agggcgccga taccgggcac gtcaggttcc acatcgccga
    18061 atacgccgac gtctggttcc gcgactacgg cccgacgttc gtggtcgacc gaaggaccgg
    18121 tggtcttgcg atggtgaact ggacgttcaa cgcctggggg gagaagtacc cggagttgat
    18181 gggcgatacc cggataccgc tcatcatgaa ccgggagatg aacctccccc tctttacgcc
    18241 cggcatcgtc atggaggggg gttcgatcga tccgaacggg cgcggcacgg tgctcacgac
    18301 ggaggcctgc ctcttgaacc cgaaccggaa cccgcacctc tcccgcgagg agatagaggc
    18361 ctacctggag gcttacctcg acgccggcca cgtcatatgg ctcaagcagg gtatcgccgg
    18421 cgacgatacc gacggccaca tcgacgacgt cgcccggttc gtgggcccga cgacggtcct
    18481 ctgcgccctc gaggagaacg aggacgacga gaactacgcg gtcctgcagg agaactacgc
    18541 tatcctccgg gcgtcgaccg accaggacgg caaccccctg acggtcatac cgctcccgat
    18601 gccgggggcg gtcgacggcg aggaccgcct gccggcaagt tatgcgaact tctacatcgg
    18661 gaacaccgtc gtgctggtgc cggtctttgg ccacccgaac gacgagactg ctcttgcccg
    18721 gattcggcag gccttcccgg accgcgaggt cgtcggtatc gactgcacgg ccatggtcgc
    18781 gggattcggc gccatccact gcgtcagcca gcagcagccg tcggtgcagc gccccgaaag
    18841 cgagtgaaca cgccgcatca ccagccgttg tagtggaccc ggtcctcgct atagcggtcg
    18901 accggcggag ggcgtgcggc cgggtggccg acggtgacga gggcgaaggg gatgacggtg
    18961 cccgggagcg aaaagaggtt tgcaaacccg agcatccggt cggcgtccgg gtagacggcg
    19021 gtccagaccg ccccgagccc gagcgcatgg gcggcgagga ggaggttttg tgccgccgcg
    19081 gcagagccct gtacctggag gccggggctc tccggggggg cgaggtcggt agagataaga
    19141 atcgcgagcg gcggaggagg agagactctc gcgtagaggc cgatcatcgg tatgcgggcg
    19201 aggatccggg ggtcgtcgat gacgacgaag tgccagggcg gtctgtcgcc ggcaaacgga
    19261 gcctgcatcg cggccgcgag gagtcttcgc acggtctccc ccgggaccgg gcctgccgtg
    19321 tactcccgca cgctccgccg ggtcatgatc gcttcgaggg catccatcag gctgccctcg
    19381 catcaatcat tcccgggaac gggtcttccc accggcatca ggtaaagcgg tgtcgtgttc
    19441 tttgggagca tgaggatctc ttcgacccgg tcatcgtcga acgccccgat ggcgaccgtg
    19501 ccgaggttga gcgccgccgc ctggaggtag acgttctctg cggcgtgccc ggcttccatg
    19561 gcgacgtagc gcgtaccgcg ctcgccgtac ttcgcggtcg tcctctccgg gacagcggcg
    19621 atgacgaggg ttgccggggc gtcgcctacg gaggcctggt cgacggccgc cgcctggagg
    19681 tcggcccgcc ggtccccgcc gccgatgcgg acgagcaggt gtccatcggg ccggtagcgg
    19741 tagacccccg cctcgaggtc gacgacgctg cctgcaacga cgtagacctc cagggggtag
    19801 agggcgccgg cggagggagt tgtccggtaa ccccggtcgt tcgtgatccc ctgcgccgcc
    19861 cagagtacct gcccgacgtc ggcaagcatg agcggcactt cggcgtagat ccgggcggaa
    19921 cgccgcttcc aaagcgcctc ctcgaccgag acgccgccct cggagcgggg ggccgggagg
    19981 atgacggcat cgtccggcgg tgccggagtg ctgttcttgc ccggcaagag gagattgccg
    20041 gcacatcccg cggcaagcac cgccgcgagc agcaggccga tggagaggat ctctggatcc
    20101 atggcacacg gagggtgccc gggcagatga aggtatcgga gcgcctccgc accccgggcc
    20161 gcaggaagcc tgccataggc gccggagctc cgggatacgc cggcgcagcc gactatctct
    20221 tcttcctccc cctcgcggac gagaagggga acttcaccag gtcgcggaga cctcccgccg
    20281 gggagaagtt ccgcatgtcc cgccggaccc ctgcgatgag tgcgtcgacg acctcctgct
    20341 cctttgcctc cccctcctcc tgcacagcgg ccaggcggtg cagggagtag agaccgagga
    20401 tgaaggcaaa gaggaagaag aactcccact gccgcaggtc aagggtgaca aagacgagtt
    20461 cccgcaccgg gctcttccag atcaacgtcc ataggagttc acgctcggca aagaagtcca
    20521 cgaagaggcc gccgagtatc ggggcaatcc cggcggcgac cgagttcgtg aaggtgctcg
    20581 ccgcgaggta accggtcgcc tggcctctcg gcgccatctt caggcttatg tttccggacg
    20641 caagcgatac ccccgcaagc gataccccca tcagtatgtg gatgaggacg agcagcgggt
    20701 aggtcaggat gtagacgtcg gggagcgtga cgaacatcca cgcgaagatc gcgagcatga
    20761 agagcggacc gctcacggcg aggaccgatt tgttggagta gcggtcggag atccggcccc
    20821 aggaccggta gaagacgatg ttcatgatct ggctcccgac gccgagggcg acgacgaccg
    20881 atatatcgag gcctatcgtc tgcagcatgt agaccgtgaa gaacggcgcg gcaaggttga
    20941 cggcgaagtt ccacgaaccg aggaatatga tgaggttctt gaagttgacg tccgtaaacg
    21001 gctttttgat ggtggcaagc aggccgtcct cctcgtcggc ggcaaccatc cggggctcgg
    21061 gcgtgcgggc aaggagggtg atgccgaaga ggccggcgac gagcccgaag aggaagaggg
    21121 cggagtagcc gtaggccgca aggtccggaa agacggtctt ccaggagtcg atgaagaacc
    21181 cggcggcaag gctgatgacg agcgcaagcg ccgtcgagag ggtcagcctc cgggagaaga
    21241 aggccccgag ccggtcctgg ggtatcaggt cccgcatcca ggagttccag ccgcagtgcg
    21301 agattgccga aaacgatgcg tagagggcga tcgacgcgat gagaacgaag attccctgct
    21361 ccggcgagaa gaagaacggt atcaggacga tgggcagcca gcagaggcgg gcggcaagcg
    21421 aggccgcgac gaccaccagc ctccgtttct ggacccggtc gacgacgtag atagcgggca
    21481 tctgcaggag ttcggcaagc ggcggtatgg cggcaagcag cccgatgacg gtgttggatg
    21541 ccccgagctg gagggcgaag gctacgagga agatgccgcc ggtcagggtc accattgcct
    21601 gggtggcaag gccgtccctg atgacgagtt tcagaccgcg ccggatctcc tcgtccgtca
    21661 ggctctcttt cgtctcaaag accatggttt cccggatatc ctgttcttgt gctcacgggg
    21721 ctcttcacct cgaagggccg gccggcgcag agtccgccgt gccggaggca cggcagccga
    21781 tcctgcaccg ggcgcccttt tctgagtccc cactatataa aaataaaatt atattaatct
    21841 gtttaaatgg atccggcggc cggccggggt cttacggcag gtctcccggc atgacggcgg
    21901 tcgtctccga agaaaacctc cccctcagct ccccgaagac cacggcaccc tgaagtcggg
    21961 atagagcgtc aggccgccat cgacgtagag tgtctgcccg gtgatgtagg ccgcttcatc
    22021 ggatgcgagg aacgcaaaga ccgcggcgac ctcctccggc agaccggcgc ggcctaccgg
    22081 gatgtgcttc tccacctccg ccctcctggc ggggtcgtcc atccactccc ggttcatcgg
    22141 ggttgcgatg gcgccggggg cgaccgtgtt gacccggatg ccctgcccgg cgtactcgag
    22201 cgcaagcgtt ctcgagaggg ccgcgagtcc cgccttgctc gcgacgtagg gggcgtaata
    22261 cggtttcggg atggtctcgt ggaccgatgt ggtgttgagt acgacgcccc cgcccccgcg
    22321 gtcgaggaag tgccgcacgg cctcgcgggc gcagaggaac gcaccccgga ggttcaccgc
    22381 gaggacccgg tcgtaggcgt ccatatccgt ctcgtgggcg gggctcgcga cctggacccc
    22441 cgcgttgttg accaggatgt cgagcctccc ccacgcgccg aggacctcgc ggaatatccg
    22501 tttgacgtcc tcctcgtgcg cgacgtcccc ctggaccagc atctccccgc atcccttccc
    22561 gcggatgatg ctgcaggtct tcctcgcctc ttcaagcgtt atctccgcct cggtctcgct
    22621 cgcgtagtag ttgatggcga cgtttgcgcc ttcgtccgcg aaccggatcg ccgtcgcccg
    22681 gccgatgccg gtcgagccgc cggtgacgag gacgttcttt ccttcaaacc gtctcattat
    22741 tcgatcgccc ctcgtaggtc cgggtccggt tgccgtccgg cttttagatg ccgcaagccg
    22801 atcctttgcg gccggatgcc tgcggggggt gccggacgtc ctgcccgttc cgggctccct
    22861 gtgttcgagg ggattatgaa gtttttccag ggcgggaagg gatccccggc cggatggttg
    22921 cacccctccg ttgtgcgttc tcgcgaaacg actgaaaagt cgtaaatagc cactgtgcga
    22981 taccattaca accagatctc gccgggcatt accggaaccg gctccggtgt tcgggcaggg
    23041 tcagggggca ggtgcgcgtc atgacgggag caggtgacgg agagttgcga tttcttgtcg
    23101 ttgaggacga cggtatcatc gcgttcgaca tcgtgagccg cctcaaggag ctcggttgcg
    23161 gagacgtttc ggtggcggcg acgggtgcgg ctgcgattcg ccaggcggag cggctgcagc
    23221 cgcacctgat ctttatggac atcgctctca aaggcggcat ggacggcatc gaagcggcgg
    23281 aggtcatccg tgaccgcttc ggcatcccta tcgtctacgt caccgcccac tcggacccgg
    23341 ccgtcaggaa ccgggcaatg gcgacgtctc ctgccgggta catcgtaaaa ccgttcacca
    23401 ccgcggacct ggtctgcgcg atcaggtctg cccttcggac tccgtagcct ccggggaggc
    23461 tacgagcata tcctcttccc caaaggtgac cgtcaccgtc gtgccaccgc tccattcgac
    23521 tgcaacgtgc cccttcaggt actcctcgac gaggagccgg acggtgggga ggccgggagc
    23581 ggcggttgcg gttatgtgca tctcctcgga gatgcccacc ccggtgtccc ggacggtgac
    23641 ggtgtacctg cccgcccggc tacggtcgat cgtcacctcc atcgtcccgg tcatcccgtc
    23701 cgggaaggca tgctgaatga cattgacgac aagttcggtg aggagaaacc cgatgaactg
    23761 tgcaagttcc aggtgcatct cgattcccgg ggcgcgcacc ttccacgcaa tcttctccgg
    23821 cggcacgccg taactgccga ggagggtgcc ggtcaggcgg gtcaggtact cctccatccg
    23881 gacccgatcg ggatactgcg accgatagag gtcttcgtgg gcgagggcga gcgagaggat
    23941 ctgcagctcc gtcgcccgga tcgctcttct ggcggcaggg tcggtgaggc aggtcgtctg
    24001 caggtgcagg atgcttgaga ccacctggag ggtattgcgc agccggtggt ggatcgcgtt
    24061 gatcagcgct tccttctcct ccagggcttt ctccagatcc tgcgccgctt cgacctcccg
    24121 ggtgacgtcc tcgagaatgg cggtcacgcc cggcgaccca tcctcgaaga ccaccgggac
    24181 gaacgttgca tgaaactgca ccgtgcgtcc gtcggccttc caggggatct cgcacttgac
    24241 ctcctgcccc cggagcgcga cccgggccct ctccccgatc tccggcggag ggaggcgatc
    24301 cggagggaat gccccgatct gccggccgac gagttcggtc ctgtcgagac ggaggagggc
    24361 ctggaacgca tcattggcga gcacgacccg caggttccgg tcgagcacca cgatcaggtc
    24421 tgaggtcagc ccgaggagtg cggagaccgg tacccggtgc gcgagggtgt agagcttcgc
    24481 cgccgcaacc ctgcgcaggt ctacctggcc tgcagcatag agcatgctca ggtacttcgc
    24541 ggtcacgttt ctcccgatgt tgagtctccg ggagatctca aggatgctga ggcccagggg
    24601 ctcctggcga aggagcatct tgatctcctc gagttctgaa atctcgtcag gcatgcaggt
    24661 acctactcct ctataccaac tctactatca tagagattgc cagtgacggg cggcggcccg
    24721 ggtaagggcg ggagttccgg gattcttgct gttggacagg cattttccgg cggtctttcg
    24781 attcgatcgc ttcgcaggag tatcggttcg taagcaatat atgttacacc aagcatacct
    24841 gcaaccatat gcatgcacat gagcatgcag atacgcgggc ggatctcacc tgaacggatc
    24901 ttcccggtat cccggaagtc cggccggggt ttgcaccccc ggctacagcc accgacgaca
    24961 gcgtggcagg ccggatacat cgccctgccg ggagaggcat ccccggtatc gctgcggtat
    25021 acgaccgccc tgaccgggcg aaaatccgcc cgacacctgt ctgccggcgc accgtttccg
    25081 gaaagaactg gtgaagaggc atgatctgtt tgaaagacgc gtcggagccc tccggtaccg
    25141 gtacctttgg tgccggtgga gaatacgggg cgggcccgga ttgggtgcgc ctgccgggac
    25201 gtgccgcggg tggaagcgtc aactccggca tgatccctgt cggcatctgt gggcgcgggc
    25261 tttcaacaat ccttttcatg actgctgcta tccgggaggt tctcctgggc cacacgaaag
    25321 gcagggatgc ctgagagggg gaaaaacggt gcctgtcccg ttgaaaccgg acgcctcctc
    25381 tatctgcgag attgcaggag gtttccggga cagagccggt agaggtggcc ggcagcgggc
    25441 gtcgggcgga ttttcgcccg gtcgaaccga tcggaggcag atcgccgggt tcctccgaat
    25501 gtatatggag ctcctcgaca gtgcggcggc gatcgtttcc gggaccgtcg caggaggcgc
    25561 ccgggtttac gtcttcctcc agcgactggg aaaagcagtc ggggtgggcc gggtctgcct
    25621 cgccggcgtc gagtacaggc caaaagccgg agaagtccgc ttacgcatcc tgtgtgaatg
    25681 gactgccggc ggaatcgccg gcaaagagga gggcggtcaa tccgttgcgt accagcccct
    25741 ccctcccgga tgggcgaagc ggcttatggc ggggttcccg gttcatgggc gctttccggc
    25801 ctcacccggt tcggtcctga tggtccccgt cttctgcggg tccgaactcc ggggcatcct
    25861 ggagtttcgg agtcgcgtgg cgcgacgggg gtggcgccga tccgatgtcg gcaccctgaa
    25921 ggcagtcgcc ggcattctcg gttcggtcat ggcgggttcc ggggcgccgc atgccgttgg
    25981 ggcctgcagc tgcgccgtcg tcgacgatcc gaccgttccc ttctgcaggc tctcgcctga
    26041 cggagcgatc gcgtatgcca gcgactcgtt ctgcgccctc ttctaccgag cgcgcgacga
    26101 cgtgatcggg atatccgttt ccgccctttt ttcaccggaa attatgaaaa aactgccgaa
    26161 tgttgccggt tttatggtga cccccgagtt ccgggccccc ggagcggacg gcggcaaaca
    26221 ttgctgccgc agcgtatgcc ggacgctcct cgacgatagg ggcgccgtct gcgggtaccg
    26281 ggtgttcggt tgcaagagta ccgaattcaa gcagcaggag gtccggagta tcgggacgga
    26341 cgaccggatg tttactctcc tggccgaagc cgtctacgat gcggtcgtcg tcctggacag
    26401 atcggccagg atcacctact ggaaccgtgc cgccgaggat ctcttcgggt acacggcgga
    26461 tgaagttgtc gggcacgatg cagggcagct gttctcgccc gagcacgttc acctcctgta
    26521 ccttgagggt ttcaagcatg ccgtggcggc tgcggcggag ggtgtcgtcc gcctgcgttt
    26581 ccccgacgtc tcgctcgtcc gaaaaggggg tgccgtgttc ccggcggcgc tttcgatagc
    26641 cgcggtgctg acgaggggcg cgtggttcgg catcggggtc gtccgcgaca ccaccgcaga
    26701 gaagatacgg gagcaggcga tcctcacgga agcggagcgg atcgggacgc tgctacggat
    26761 agcggcacgg ctgaacggcg gcgtcccgtt cgacgctgca ctcgatctgc tctgcgagga
    26821 gacggcccgg gcgctccggg tttctggcgc ggttatctac ctctacgacc ttcctggcga
    26881 cctgctcgcg ccggcccggt cgttcggtgt gcccgaggag gtacggaagt ccctgccgcc
    26941 catcccgcag gcactctacg agcgagaggt cgaacgacag ggatcggctt ttgtcatccc
    27001 ggacaaaagg ccctggacgg acgtggcgtc cggaaaaaaa atgccgggga acccccttcg
    27061 accgctcgcc tccgcagcaa tcctccacgg acggaccctg atcggggccc tgaacgtgat
    27121 gacgctcgac accccgcgca gcctctcccc ggaggagctc acgctcctga aagggattgc
    27181 agacctggct gcgcttgcaa tcgccgacat ccgcttttcc gcggagcggg cagggcatga
    27241 acaccagttg caggcgttgc tggatgaaaa gacggccctg ttgaaggagg ttcaccatcg
    27301 ggtgaagaac aacatgcagg tgattgcaag ccttctctcc ctgcagggcc gcctgacaga
    27361 gaatgccgtg gctcgtgcat gcatccggga gagcgagaac cgggtgaagt ctctggcgct
    27421 cgcccatgag aacttctacc ggtcggagtc gatcgccgcc gtcgatgcag gcgactatct
    27481 gctccggctc gtaaacgacc tgctccggct ctacgccccg ccggtgcatg tgtcggtcga
    27541 tgttgagacg gagaaggggc tcgtcctccc cggagacgtg gcgatcccgc tcggcctcct
    27601 cctcaacgag ctcgtctcca actctctcag gcatgggttt gccggtcggt ccgaaggggt
    27661 gatcgcgatc cggatcgtcc gggcgacgaa ccgccgcctc accatcgagt accgggacga
    27721 cggcgccggc atacccggtg aattcgccgc ccggcccccg gcgaccctgg ggctgcagct
    27781 cgtccggatc ctcgcggcac agctcgaagg aaccgtcacc ttccggaggg gcgagcccgg
    27841 gacgatcgtg gagatcacta tcggagacgg cgccggcgag gggaggatga accatggata
    27901 gcccgagcga tacgactgag gggcataccg tcctcctcgc ggatcccgat cccctcaccg
    27961 ctgcaacgct ctccctccgg ctgcaggagc tgggatacgc ggttgtgggt gcggcagcga
    28021 agagcgacgg cgccctggcg gcagcagccg cattccggcc ggatgtcgtc atcattagcg
    28081 ccgatctccc cggtcccggg agcccccggg agacggctct cgggatccgg gacgggctcg
    28141 gcatccccat cctcgttacg gccgcacacc ctgtagacac gctctccccg gacggggagg
    28201 aggggctctc gccgcaggag atcctcgtcg ggctcttcgg cccggacgac cttgcctgcg
    28261 cgatcgaggc cgttatcgcc cgccatcggg cccggcaccc gtgccgggaa tgggtcgagg
    28321 cgttcaggca ggccgccgag atcgtgccgg gtttcatcat gctcctcggc acggatcgcc
    28381 acatcaggtt cgtcaacctg tccggagcgg ccatggccgg acgcccccgc gacgcgctta
    28441 tcggactgga tgccgtcggg acggccctgc cgttgtccga tcgggacggc ggcgaccttc
    28501 ggcggcgttt cgacgaggag caggagttcc acgttgccgc cgactgcata cctatcggca
    28561 ggtgccgttt ttccctccgg tggacctgcc agccggtgcg ggatggccgg ggatgctgtg
    28621 ccggctgggt ctgctccggc accggagagg gcgacggagc ctctccggat gcctccctgc
    28681 aggcggagat tctcctgcgg tttgagaaga atatggagca gcttgcaatc ctcaacgatc
    28741 ggattcgcaa cccgctccag gtgatcgccg catttgccga attcgcggac gacgacattc
    28801 gcgataagat ccttcttcag gtgaaagcga tcaacgagac cgtccggcag ctggataagg
    28861 ggtggcttga atcggcaacg atccgggagt atctccgcaa gcatcacagg atggcgctgt
    28921 gaatgcgccc cggaggctgc accgtcgagc gattcagcgg gatctctcga tcaccttccg
    28981 gacgagcgcc cgccggtccg gcctccccgc cgtgagcgcc tggaacttct cctcgtagat
    29041 cggcctcctg accgagtaga acgtcccgag cgctatcggg gcgtcgcggt cgtaatccca
    29101 ctcacggatc ttatctagag cgctctcgaa cctgcccgtc tcgacgtcgc cttcctgcat
    29161 ctcgtaggtg tgctcgttgt agtagtcggt caggttgaag taggtcgcgc agatctggag
    29221 gacgtcgacg aacgagaacc cccggtgcag tatcgcctcc ttgaagaggt cttttaggtg
    29281 ccggaccttc ttcgtgtagc cccgggcgat atgggtggcg ccggcggcga gcatcaactc
    29341 gagggggttg atggggtgct ccgatatccc gccgggcgtg gagcggccgc ggaacccaaa
    29401 cggcgacgtg ggggtgtact gcccggtcgt cagcccgtag acccggttgt cgtggacgat
    29461 gacggtgatg tcgctgttgc gtttggccgc gaagatgagg tggtcgagcc cctccgcgta
    29521 gatgtcgccg tccccgacgt ggcagatcat cttgaggtcg gggtttgcaa ggcgcatgcc
    29581 ggtggccgcc ggtatcgaac gcccgtgaat gccgtaaaat ccgttgacgt ttaagtagtc
    29641 gaccagtttc ccgtggcacc cgatcccgcc gaggaggacg aagttgtgga gcggtatccc
    29701 ctcctcctcc gataattcgg cgatcgccga cttcatcatg tgctctatcg cgaagttgcc
    29761 gcacccgggg caccaggtgt tcgctgccgg gctgatgagc ccgccggccg gtctcccggc
    29821 agatcgctgc gggctcatgc gagcacctcc cggaggcgcg cttccagttc gtcgaccgca
    29881 aacggccggc cgtcgtactt caggaccggc cggccggggt cgaacccctc ctgccggagg
    29941 aggcgggcaa gctgtccggt ggcgttgttc tcgacgcaga cgacctttcc cgccccgatc
    30001 atcgcctcct tccaggcacg ggcggggaac ggcgcaaaga cgagcggctg gacgaccctt
    30061 gccccgaacc gttcggcggc ctcgatgcac gcccccttct ccgagcccca gcagacgacc
    30121 gttgcgccgg cgttgcgggg gccgtagatt ttcaccgtgg ggtaggccgc gagttcggcc
    30181 ttgaggctct cgcccttcgc gaacctcttc tcctggagag ccgtgacggc cccggcctct
    30241 tccgtggtga acccgacctc gtcgtgggca tagctgttcg ccctgacgac ggcgccctct
    30301 cttcccggga acgcgagcgg cgagactcca tcatcggtcc gggcataccg ccggtactcc
    30361 ccggccccgt cccagagaac gggttccagg tcggggtgca gggcgatctt ctcgatatcg
    30421 aacgagtagc cgccctccgc gagggtcttg tcggtcagga tgatcgaggg cacctggtac
    30481 ttccaggaga gcatcagggc ggtcgacgac caggtgtagg cctcctcgat gtctcccggg
    30541 gcgacgacga accgggggaa ctccccctgg cccgcgttca gaacgaagtg gaggtcggtc
    30601 tgggcggtgt aggtcggcat cccggtgctc gggccggggc gctgccccat gacgatcgtc
    30661 acgggtatct ccgccatacc ggcaaacgag agaccctcgg tcatcaggca gaacccgccg
    30721 cccgatgtgc cgaccgccgc cttctcgccg gcgtaggcaa gaccgagcgc catcagtatg
    30781 acgcttatct cgttttccgg gtggaggacc ttgatcgaga ggtcttcggc ctgcccggca
    30841 aggaagtgaa ggaggtttga cgtcggtgtc atcgggtagg cgacgtagga ctcaagcccg
    30901 ccgtggacga gaccgagccc ggcggcctcg tttcccgtca tgatcgggag ggtcggggca
    30961 tcgagcggtt cgacggagaa ggcgctctcc acgccgtcgt atccccggcc ggcgaccctg
    31021 aggtttgctt cgaggtgctt tgccgggacg ctctcgcgga ggacatcccc gaggacaccc
    31081 cggcctatgc ccgccacccg ggcgagggcg ccgagcatcg cggagttccc ggtgatcggg
    31141 ggcgcgtcct cctccctgac gatcgtatcg atcgggaggc cgtagccctc gcctttcgtc
    31201 acctgcgagg cgtcgtagat gacggtcgtg ccgcggtgga tcctctggcg gtggttctcg
    31261 atgctgttct ggtcgaaggc aaggaggacg tcgaccggag tgcggtgcgc ccctatcggc
    31321 cttgccgagg cacggatgat ggcgaagttg tgcccgcccc ggatgagcga ggggtaatcg
    31381 aagtacatgt aggtgcggta gccgaggcgg gagaagaggc gggcgatgga gaggccagcg
    31441 gtattgatcc cctctcccgc cttcccgccg atcagcacgg agtattcacg catggttatc
    31501 aaatgtgcct ggcctgccgg cggatatata cgttgtgccg ccggaacgca tagccctgga
    31561 ggctccccga tacggcctca cggcgacctc tcgcgttgcc ttgccgggca attcccggca
    31621 accctctgcc ggaacctcgc gggagccgac agcaggcatc cgccacctca ccggacgacc
    31681 ggcgacgccg tctcgtcgaa ggggttcgag cgcatcgcaa gcgagaagag gtaagggtag
    31741 ttcttctgga ggtactccat gtaggcgagc cactcccggg cgaggagacg gtatacccgc
    31801 tcgatatcca cggtgaggtg gcggatgtcg cttgccggaa gcccggcgaa gtcatcccgg
    31861 cgttcgagtt cttcggtcag gtggaagacc gccctgagga gatcggtgaa ggactcgtgc
    31921 tccaggagag cagggttctc gagcagccgg aggaagaagt cccgccgccc ttgaaggtag
    31981 cggcggagat cctgcaggtc cgcggcatcg acggcgatcc gggcgtggtg gtgctggagg
    32041 cggtcgcaca ccccggcaaa cgtctccggc gtccaggcct ccgtcacgat gagcccttcc
    32101 ctgagttcgg gcagttcgga gtcgcgatcc gatatgaagg cgaggagcca ggtcccggcc
    32161 tcggagaaga aggtcccgat gaccatgttc agtttctcga gcctctcgcg ctttgcccgg
    32221 atgctcagga gctggttcaa gataagggtg acgaggagga cgttgatcgg gaggaacccg
    32281 agggcgttga atatgtaggt gtaggtgttc tccggatcgc cgagcaggag gtactttacc
    32341 gtgtagatgg ccgccgacga ggcgaccaga acgataccga ggcgtacttc ccatgtccgg
    32401 agggtgagcg gcatgcgcag gggaatgtac gcacggcggg gcgaataaag gttgggccgc
    32461 gggggccctt cagacccgga ggcgcgacgg cgccggcacc ttgaggggca cgggaacctt
    32521 ctcttcccgc gcgatctccg ccctgaccca ggcggcaagg tcgaggaggc ccgcctcgag
    32581 ggagacctcc ggctcgtagc cgaggacctc gcgggcccgg ctgatgtctg ccgtgcagtg
    32641 gcggatatcc cccacgcgcc ccgtccccgt gatgtcggga gagaggtcct gccgcccggt
    32701 tatcgccata agccggctcg cgacggtcca aaacgtgtga ggtcggccgc tcgcgatgtt
    32761 caacgtctcc cccgctgcac ggggggactc gagggccagg cgaaaggcgc gcgccgcgtc
    32821 gtagacgctc acgaagtcgc gctgctggta gccgtcctca aagaggcgca cgggaaggcc
    32881 ctggagcagg cggaacgcgt actcggtcag catgccggag taggggttgg catacccctg
    32941 gtgcgggccg tagaccggaa agagccggag gatggtcgtc ggtatgccgt aggtctcgcc
    33001 gatcatccgg cacatctctt cctggtcgta cttggagacg gcgtagaccg aagacggcga
    33061 cgcctccttc gtctccggcg tgggaagggg gtagatcacc tcgccgtccg ggctccgggg
    33121 ctcccagtcc ccctttgccg cccacccggg aaaacgccgg accttcgggt agacagtgcc
    33181 gttgtacgag cagtagagcc cttccccgta gatggcgctg cttgaggcga cgacgagccg
    33241 cctgatgggg tggtccagca aagcctcgag gaggacggcg gtcccggatg tgttgacgct
    33301 caggtacttc tcgatccggt acatgctcga ccgctccccc acgaccgcgg caaggtggat
    33361 gacggtgtcg atcccgtcga gagcctcccg cagccggtga ggatcccgta tgtcgccgac
    33421 gacgacctcg gcccgccggt caaggtgccc gggctcccgc ctatccgggc cgtgcatccg
    33481 cggcgtcagg ttatcgagga tgcggatatg gtagccgttc tgaagcagtt ccgttgcgat
    33541 gtgcgatccg atgaaccctg cccccccggt gacgaggagg gtcggactgg tatccgtatg
    33601 catggtgtat cagggcacac ctgatgcgct ctttgcatta aatattatcg cgaatgcgaa
    33661 atattaatga atcccgtatg ttccagctat cgaggtcgtt ctccaccttc tccgatccgg
    33721 tctacccggc cgggccgggg aagggcggcg cccatcccgc ctccggttca cgagtcaagg
    33781 atcgcctcgg ccgcatcact gtaggcccgc atgacgtacg gtatgccgga gaccgcctcc
    33841 gcctcctccg tcagggccat caggtcgctc cgggcgaccg tcccgaggct gaagttccgg
    33901 gttccggcca tgatctgctg gagccccgtc cgaaacttct gggtataggt gtagatcccg
    33961 accgcgccga gcgggatctc gccgatccgg tcggggaacc tctcccggag ctcctcgtag
    34021 cagacgaaga tctcctcttt cgtgcggccg tacttcgaga cggtctttgg gagttcgccc
    34081 gcctcgatcc acgttgctat gttcttcccg accatgccgg gtatcatgag ggcgcggccc
    34141 atgcagacgg cccggacgta ggggctcccc atggcaagcg ccttgaagac gttcgcttcg
    34201 tcggcaaacc cgccggcgat ggcgatatcc gggacccgga tgccccgctc ccggagccgt
    34261 tcgcagaacc ggcaggcgag ggactcgatg tagaaggtcg ggatgcccca ctcgttcatc
    34321 atcggccagg ggctcatccc cgtgccgccg ggcgcgccgt cgatcgtgag gaggtcgatc
    34381 tctgcctctg cgccgtagcg gatcgccatc gcgagttcga cggccgagta ggcgccggtc
    34441 ttgagggtga cccgcttgaa accgatatcg cggagccggt cgacctcgtc gaggaacccc
    34501 tcgcgggtga cgaaacccac gcgggagtgc cgctcgaact cccgtagagc gccggcctcg
    34561 aaggccttct gcacctcgtg gagcccgggg tcggggagga cgatgtagcc ccggtccttg
    34621 agccggttcg cctggtcgat atcgccgacc ctgatctcgc cgccgatgca ctttgcgccc
    34681 tgtccccact tcaactcgat cgtctccagg ttgtgctttg cggagacgta ctcggcggtt
    34741 ccaagtcgcg tatcctcgac gttcaactgc acgagaagtt cgccgtaccg gtcgtgaaac
    34801 cgccggtaga tctcgatccg gcggtccatc tcgggagacc tcgcgaccct gccggcgtgg
    34861 tcgagcacca gcccggggtc gacgccgcag acgttctccc cgcagacgag cgtgatgccc
    34921 gatatggcag ccccgatggc gaactgctcc cagttcgcgc gggcgctctc ggtcgacccg
    34981 agcgccccgg tgaagatcgg gacccgcatc gggactttcg cgctccaccc gtactcggtc
    35041 cgggtatcca cggcggggaa tacggcggcg tcggggccgg cctcgatacc ttccggcagc
    35101 cctttcgcgc cgaccgcgta gccctggatg ttgaggtgcg agtagtcgac cgggtagttc
    35161 ttatcggcgc cggccgtgat ctcgccgaac gggcccgggt agagggcttc tctgccgcgg
    35221 aaggaggaaa gccagatctc gcaggcccct ttgcagccgt cgatgcaccg ggtacagatg
    35281 cctgatgcgg gcacgacgtc acgggaacgg ttgctggttc ccgtcgcttc gttcgcgttc
    35341 ggtcttcgca ggttcatcgt tgcaccagtt gacggataga aatttccttc caccatatat
    35401 aaatattcct atcggccggg gcccgcaccg gtatttatac cgtcgtatcg ccggggtgcc
    35461 gggagcatga cgaaagggta aaatatgttg gtgtacatga ttggcttatg aacttcaggt
    35521 tgctacctgc tctgatagtg ctcctgctcg cgttctcact cggtgccgcc tgcacgggct
    35581 ccggcggtga agccgggccg tccacgccgg gaaccgggac cccggcggcc acggcaactc
    35641 ccgcaccgac gacggaggtc tcgcttgtgc ccggcccgac gcagacgata ccgccgggca
    35701 aagaggttac gttccagatc acgcccggat atccctcccc gttcacgcat gacctcacca
    35761 tcaccttcaa cggcggcaaa ggtcaggact acgtgaacgg catcgcgctc cgcgtgacca
    35821 agtcgaacgg cgaggtcgtc gagaagacgc tgcagccgcg catcggcgac gcggtcacca
    35881 tcaatgaggc agaaggggat aaccgggtcg agataaccgt ctccctcgtc acgggaggat
    35941 cctacaaagt taccgacgag gtcgtgaaag tcccctgaat cccccgacct ctattttctc
    36001 gcgattatag cctcaatccc gggcagccga ctctttctcc acgctccacc gtacgaggag
    36061 accgtcgtcg agccgctcga tctcgatgat gcgcagccgc gtaaaatcca cctccctggt
    36121 aaaaccgtcg ccgtcggccg gcgtgggagc atcctttccg ccgatgacga tgttgccgac
    36181 gaacgtctgg agttcgtcga cgagcccctg cgcaaagagc gcccagatca gggtcccgcc
    36241 cccctcgacc atcagccgcc tgaccccctg tcggtagagt tccgcgagca gggccgcgag
    36301 gtcgaccgtc tcatcgccgg cgatgacgac gacggcgtgt ttgccgaggg ctgccaccct
    36361 ctccatgtcc gccctgcgcg agacggcgac gatccgcttc ccggctccct tgtggaggat
    36421 atccgcatcc ggaggagtcc gcccggcgct atccacgacg acgcggatgg ggtgctcgtc
    36481 cctcccggcc gcccgccgcc cggcccggag ttccggcgat ttgacggtga gcgaggggtt
    36541 atcggcgagc acggtgccga tgccgaccat aacggcatcg ctctccgcct tgatccggtc
    36601 gacccgtgcg aagtccgtac ttcccgatat cctcacctgc cgtcgctctc gtgtcgatat
    36661 cttcccgtcg gcactcatgg cgacgttgac gaagacaaac ggccgcatat tgtagaacta
    36721 tgcaacgatc cacatatatg ctctttcctg cagggtactg cgcggcgcct ctcctcctcc
    36781 ctgaaccggg aaacgttcga catacggctc cccgggaggc atgcgcatac atttcgttcc
    36841 gggaaagatt atttaaccct ttacagaata cttcgaacag catatatacc cttcccggag
    36901 aattctctgt atccctcatg accgtaaacc ggcgttttcc gggccgtctc cgggaagatc
    36961 tcttcacagg gacggatctt gtcaagatga tcgtgatact gtcgttctcg gcagtctcat
    37021 tcatgctgac ggtgatcgca ccggattacg gcatatatcc cgcaaattcc ctgatatact
    37081 gcatcccgat cctcctcgcc gctctctggt atccagggca agggttcagg gtgaccgcgc
    37141 tcctggtcgc cgggttcgcc ctcatccgca tatacttctt aaccctcggc ttcgccatag
    37201 acccggtgat gacggggctg aacatgctct tcttcttctg ggtctttggg gcgacggttc
    37261 tcttctccca ggacccttac ctgaccatct cccggtgcag gcaggtcgtc gagaatacgc
    37321 gcgatgcgag gcttctctgc gaccccgaga ccctccgggt cctctgtgtc agcaggcggt
    37381 gtgccgatat actcgggtac gcgccccccg aactcatcgg gatcccggtg gagaagttct
    37441 ggccggacga gagcgagagg gcccggttca tcgcggagat gaagcgggag ggctacatcg
    37501 gcaacatgga ggcgacgttt cgcaccagga acggcgacgc ccgtgcagtt ctcatatcct
    37561 gccggtcgct ggccgacgag aacctcgttt tatgcacggt cgtggatatc ggcggacacg
    37621 ccgcccttgt ccaggaaaac gggcggttga tggaactcat tcaccagtct aacgacatat
    37681 ttttcatgca ggatgttgcg ggccgcatcc tccacttctc ctggctgcgc gcccccgagt
    37741 acgggatctc ccctgaaggt ctcatcgggc agggcgtgga tgctctcctc tccgctgacc
    37801 tcgccaggga gcacgcggca cggctccagg atgtggtcga gggcaaaaag agcacgtgtt
    37861 atgatatcag cctggaactg gcgggaaccc gccacgtctt ctccatcacg atggccccct
    37921 attacggggt ggacggcacc ctcatcggtg tcgccggatc ggcgcgcgac atcaccgagg
    37981 tgtggcggca gaggcttgcc tgcaggcaga tggccctgga gatagaccag tggaaagggc
    38041 tggttgccac cctctcccac gagctgcgca caccgttgca gccgcttatc gggcacctcc
    38101 agatgatcgt cgaggatccc gggtattacg gcctttccga agaggcaaaa gggccccttg
    38161 cgacctgcct tgcgtgtgcc cggcaggaac aggcggcggt ggagaagatg gtcggattcg
    38221 gcttgcttgc agcggattcc gtcgaactcg tcattcagag cgtacctctc cggtcgctcg
    38281 tcgattccat cgtctcctcc ggcggttacg accgggaggc ggagttttgc aacgatatac
    38341 cggagaccgc ctctctccgg ggagatcgcg atcggcttta tgaggtgata gagaccctgg
    38401 tatcgaatgc agttaagtat aatgaaccgc caaagaaagt atgggccagc tacacagaat
    38461 cgaatagaaa ccattatatt acggtatgtg ataatggcat cggtatctcc gcggatgccg
    38521 tcgaatcgat cttcgggccg ttctctaccg ggggcaccga gcaaccgaac cgggaaagga
    38581 ggcaaacgga tcctggcctc tcgatcgcaa ataagtatgt tcgactgcac ggaggggata
    38641 taacggtgag aagcgtggtg ggcgagggga gcaccttcac catcaggata ccaaaggagg
    38701 tgtaaaagag tgggagataa gatcatggtc gtcgatgacg acctgccaac actggaagta
    38761 atggagctgc tgctcaagaa gatcgaccgt gagccgctcc tggttcacaa cggttgggac
    38821 gccttgagga tgctcaagaa ggagaaaccc gcgctcatca tactcgacgt gatgatgtcg
    38881 cccatcgatg ggtggcagtt cctggaggag ctgaagcgca acgaggagtt caaggcgatc
    38941 cccgtcctgc tcttcaccgc aaaacacgtc tggcccgagg agtactcccg atatgcgggt
    39001 gacatcgtcg gggttcttga gaaacccata tcgctcgccg agttaaagac ggctcttgag
    39061 agggcgcttc ccgggagcgc cgcttctcac agagataatg atactcacgc acgaacacag
    39121 gttcgaagcg ggtaactttt ttcccgaaaa ccccctgaga agatcttccg tccggcgata
    39181 ccggtctgct tgcaccctcc accgaaaggc atttcacttg cccggggtgt aatacgggca
    39241 ggaaccccga tgatcgttac ggatctcccc tgctgcccct ctctcaaaga gtaccgccag
    39301 atcgtgggag cagaacagtt ctcggccatt gaagagcttg ccggccgcct ttcgggcata
    39361 cggatgcagg agatcaactc gacccgccag ggcggcggcg tcgccgagat tctggcttcc
    39421 tacgtcccct tcctgaacgc gctcggcctc gagacgacct ggagcgtcat ggaggcaaaa
    39481 cccgcgttct tcgatgtcac gaagaccctc cacaacttcc tccagggccg ggacgggttc
    39541 tcgccatcga tgatcgagac ctactgggag gcccagcggc agaacgaagg cctgatcgag
    39601 gatgacgccg acgtcgtcac catccacgac ccccaaccgc tcggcctcat cgagtttctg
    39661 accgggaggg agcgtgagca aaagaggctg ctctggcggt gccacgtcca gctcgagacc
    39721 gtccctaccg ggaccgcagg cagtatcggc aacatcctgc gccggctggt cgagaagtat
    39781 cacgcaagca tattctccag ttttcagtac cttcccctct ggcgcgtgcc gggcttcatc
    39841 ataccgccgt tcatcgaccc tctctccgag aagaaccgcg accttcccac ggccgaggtc
    39901 gatgccgtcc tcgcgaagta cgggatcgac gccgaaaagc cgatcgtcac ccaggtctcc
    39961 cgctacgacg tcttcaagga cccggtcggc gtcatcgagg cgttccggaa ggtccgccgg
    40021 aagacgccct gccagctcgt cctcgtcggc gggcgggcgt gcgacgaccc ggagtgtttc
    40081 ctcgtcctgc gcgaggtccg cgagacggcc gaggacgacc ccgatatcca cgtactcgac
    40141 ctcccgccgg acagccaccg ggagatcaac gccctccaga gggcgtcgga cgtgatcgtc
    40201 cagaagtccc tcaaagaggg gttcgggctg accgtcaccg aagggctctg gaaggggaaa
    40261 cccgtcgtcg ccggcaacgt cggcggtata ccgctccaga tccgcgacgg ctggaacgga
    40321 tacctggtct cgacggtcca ggagaccgcc gaccgggtac tctacctcct ccggcacccg
    40381 gatgcggccg gagagatggg gatacggggc cgggagttcg tgcgggagta ctacctcctt
    40441 ccccggggcg tgcaggacca cctcgcggtc gtcgaccagg tggtgaacgg caagatcacc
    40501 gcccgcgaga gcgtcatctg ctaccacccc caggtggtga cgggcaggat ggcggcatgt
    40561 gcgccccggg attgacgggg cgctccctgc cctgttatat actatggcgt gctgagggtg
    40621 ggcccggtga taggtgatga agatagcgat tgccagcgcg ggggcggggg ggcttgacga
    40681 tacggtgtcg ccggtctttt gggcgtcatc ctcgttcacc ctgatcgatg cggagaacca
    40741 ggagatcctg gccgtggaga tcgtcgtggc tccggagggc tgtgcacccg gggcggccgc
    40801 tggccggttc gcgcgcgacc ttgccggccg gggcgtggat gccgtcattg caggggagtt
    40861 tttgccggaa gagaccggcg tgttctcgtc ggccggcatc ggcatgtatc gccttcagga
    40921 tatgccgatc cgggatgccg caaagcaggt tttgaacctt gccgctgata ccctgcgatc
    40981 tcccgggcgc ggggccgtgc cgacggacta cgtgacccgg acctacggtg gaggcgtcgg
    41041 ccttggcagc cggcggcgga cgggcggggg gccgcggtag ccccggtcac ccgatgggga
    41101 tctctcttcc gccgggtccg gatccgccct ccaccttctt caggctcacc tcaaggacgc
    41161 cgttcctgta ccccgtcgcg gcgccttcgg cggtcacgct cgcgggcagg cgtatcaacc
    41221 ggctcagggt gccgtctccc gccccctctc cggcggggcc ggcgcgcctt gccgttatcc
    41281 gcagggtctg cgggttgggg agccggatgg cgatcgtctc ctcatcggcg ccgggaagtt
    41341 ccgcgatcac gatcacccgg tcgccgcggt cgagtatgtc gacccggaag cctgcggaga
    41401 ggatggagta gtcgcgggcg gtcgcgggca gactgtcggt ctcccgccgc ccggtgccgg
    41461 atggctcgtc ttcgcttcct gtcatggtcc ctctctctcg atcggatggc cggccggcca
    41521 cccggcggta cctcgtctgc cggcacactt aaccctttgt accgggggcg gcctgcctaa
    41581 ccctggcggg atatgcctcg cgaatccagg aggcccggcc gccaacagac tcttctgtca
    41641 cgttttttca tcggccgtta ggtttttgtg gtgtgaccgg tatggtgggg aacccccccg
    41701 gtctttcggc gtgcagggtg atcggaatgg cctggccctc ttcaccgctg caccgccgga
    41761 gtgtccgtac tggtgggatc ggcggggttc tggaggagag agaatggctc agtttaaacc
    41821 gttcgatatg gagggcatgc ctcttgaaga gcagcccatg acctggaagg atatgtctac
    41881 ggccccgtat gacaagaagg cggtcgacgc ctacacccgg acccgtgtca tcctcatgaa
    41941 cgggatcgag aacaacgccg tcctgatgtc ccacgccgtc gcgcggatgc accccgaacc
    42001 cgaggtgaag aaggcgatgg ccatgatacg gcggatcgac tcccagcacc aggtggcgat
    42061 caactggctc aacccggcgg accagtcggt catcgagacc acgctcggct acgagcaggt
    42121 ggcggtcgat cttacggcga acctggcgaa gaacgagccg gatccctacg tgaagcagac
    42181 gctggacttt gcgctgctcg aggacttcga ccacctctac cgctacagtt gcctctacga
    42241 ctacatcgag ggcggcgacg ccgaggcgat cgtccagggc aagaccgatg tgaaaccggg
    42301 ccggccgacg agcatcgaac accgccaccc agacgactcg atgcgcaagc actacgataa
    42361 ggatacggcc gacatcaaga cgaagatgaa ctacgtcacg atcgtcgccg gggagcagca
    42421 gacgatgctc tactatcggg cgcacggatc catgtacccc gacgatatcg cgcggcgact
    42481 gtatgccgag atcgcggaga tcgaggagca gcacgtctcc cagtacgagc tcctcggcga
    42541 tccgcgggag acgatgcttg agaagctggc gctggtccag ctcaacgagg cttacctcta
    42601 ttactcctac gcgcagcacg agagcgaccc gaggatcaag gggatctggg agacgcatat
    42661 gaagatggag atcgcgcact tcaacgagtg cgcgcgactc atcaggaagt tcgaggggcg
    42721 cgacatccat gacatcctga aggccgacgt catcgaaccg ttgatcgttt tcgagtcgaa
    42781 caaggattac gtcgaccggg ttctcgatgc gcagctcgat cttatgccga acaacaggga
    42841 gtacgtgcgg ctccgcgacc ttcccgacga ctgggcaagc ttcggctacc aggcgaaggt
    42901 gaacgcgaag ggcgccccga gcgaggagat cgtctcgaaa gccggccggg agcttgcaca
    42961 gcgcgatcag gcggagaaga tcaagaagat caagcaggag atggcacgga agatggagaa
    43021 gggcatggcg gcagtgccgg cccggtgaac acttttttgt gtggcggccg gggcagggat
    43081 gtacgtggac acgtgctgcg ccctgacccg gatggtgttc tgcaaccggg gcgtgttcta
    43141 tcgggtcctc acggcccgcc ccggcggtgg caacggggct cctcacggcc tgataatcgt
    43201 catggcgcta cttgctccga tcgatatatt tataatatta cgcgcatatg agtaatatgt
    43261 cacaacagag gtgaagaaaa gatgccagga tttgatggta ccgggccccg gggaatgggg
    43321 ccgatgaccg ggcggcgcat gggccggtgt gccctgccgc cacagacaga aaacagccct
    43381 gccggggaag cgggcgaagg tgccccggag actccggttc agcagccgca ggtctacggc
    43441 cttgggcggg gcggcgtccc gtggggatgc ggcagaggca gaggattcgg cggcggcgga
    43501 cgcaggcgct gccggtggta aacgcctgat gcgggccggg ttatcatccg gcctgcaccg
    43561 gcaagagcga gcatggccgg cagtgattga cccgggcgca agacctgtat ggatcggaga
    43621 tgtggcggac cccgccggca cgaggcgggc aaacggcgga agtattctgc ctgaatgtta
    43681 acgaatacgg gcgaattcac tcccggatcg gatcttaccg gcggatctct tgtttccgga
    43741 gttcggcagc gcgttttgcg cttcgcgggc aaaagcaagc gttttttccg ggttgcggag
    43801 gccgtctttc ttgatgccgg tatgcacgtc gacgccgtag gggcggacct tccgtactgc
    43861 ctcgcggacg ttctccggcg tgagccctcc ggcgagtata acggggattg tcgagtttgc
    43921 cacgatcttc gcactgatgt tccagtcgtg cgggataccc gtccccccga tccgctcttc
    43981 tgtcctcgag tcgaggatga gggcgtcggc atagggctcg tagagttctg ccgtcgctat
    44041 ggccgattca tccgtcacgt gaacggcctt tacgatcttc acgtacggga gggcaccgcg
    44101 gatggcggag agttcggacg gttcgacggt attctgaacc tgcagcgtcg tgcagtgcga
    44161 atctttcgcg agcctgacga ggtcggtcgt ctcctgcagg tgggtgaccg cgaccggttc
    44221 gatgaacggc gggagatgcc ggatcatatc cgcagcctct gccggcgtca cgacatcggc
    44281 gctccggtag gtcacgccca tgatgaaccc cacggcgtcg caaccggcat cgaccgcaca
    44341 ctgcatatcc gtaaggctcg ccgttccgca gaatttgatg cgcatcagat cctctctgtc
    44401 gtccgggaag atagggtatg cggaccttca aaggttgccg gccctgcacg agcggtgaga
    44461 tgaggggctg ctcccaccgg ggaggtcccg gcagagccgg ctctgctgct ccggttctcg
    44521 atcgccggcc cgttacgacc gtgccgggac gagatccgcg atggcgatgg tcggctcgtg
    44581 ttgcgtgagc ccggtgacct tctccgaact tctctcagcc ggtagcgatc atcggcacct
    44641 cccccgcgag ggagtgtttt tcggggtggg acggcagcgc tgcttgctgc cgggggatcg
    44701 ttcggcggta ccctcacgga tggccggttg ccgataggtc tttcttcagg ctgagaacct
    44761 tccgaagttc ccggcgcgag atctcaccgt caagaaccgc gacgatctgc cgccatctga
    44821 tacggcaaat gccccgccca cctcaagagg acatacggcg cgccccggtc tcttcggagc
    44881 agagtcttga tgggaagttg ttcgtccagt ccgtagcgcc gatctaccct tcctgcatca
    44941 agaaacgggt gaaggggttg atcaagacct gcacgctgcc gggtttgttg gacaggctgg
    45001 atatcatcga gtggtttgag taccccggcg ggtgaggtgc gggatgcgca agcccggctg
    45061 tactgtggcc ccgggatagg tccgccagcc tcgttatgag gggcgggagg agaggattca
    45121 catcaaccct gttctgcatg ccaccgggct acctcgcgtt ttacggtaga atgactcaac
    45181 gagtagccgg atgcaacgag gtgctgccag atctccctgg cattgcaccg ctgcttcttc
    45241 ggtttcctgc gattcgcttc gaggatgcgg tggatcagcc ctcggatctc cggcgtgacg
    45301 acgcgggcgg gtcggtgaat cttgcggtca cggggtacga tctcttcgat cgcgccgtcc
    45361 cgaacggcct gggcgcggag gacatacttc cggacggtgt tatacgcgac acccatttca
    45421 cgggctgtct ggcgataaga tcccgtttgc tcatatgatt cgagtagttt ttcgaccgtt
    45481 tccagggaat acatctctcc gctaccccct cacgcgggtt gcatgagaag aatatggttg
    45541 tgggtggtca aaaacctact ttccgcggta acaacgacca cgatcctgaa tcgatcaacc
    45601 gagccgggat aaccttaagc cagggtacta cgttcacaca ccgatacaag tgttattagt
    45661 taactccctc catacttaaa cgaaatggta tcagaggaag ttatccctga atccttcacc
    45721 aaatgcctgg ctcgtctcgg gatcattgct gcgcttgtcg aagggtttgg catcgtcgac
    45781 ctggtcaacg cacggctgcc gagacttcag catcgcaccc tgacccatgg cgatgtcgtc
    45841 cttgcgctca tcttgaacgg tcttggcttc actcagcgct ggctctacct gttttctgcc
    45901 tggttcgaga acaaacccct ggaacgcctt tttggaaaag aggtcacggc tgccgatatc
    45961 accgacgacg tcattgggag aacgcttgac gccatcgagg agtatggccc ggaacgcctc
    46021 taccaggaga tcatcagcca gatctttctt gtgacccggc tcgggtatca tcaggtgcat
    46081 cttgacatca ccagcttcaa cttctacggc gcctccgata ccacctcgac ggtccacgtc
    46141 gcacagggct atcccgaaga cgggcgatgg gggttgaacc ggtctggact gggtctcatc
    46201 gtgaaccagg ccggcgtccc gctttccatg agattcctct ccgacaacgg cgacgataag
    46261 gttgctcttc cggagatgct cgccaggttc cggagccggg tcgagttccc ggaaccgctc
    46321 tattacatcg ccgatcctgc gtcttatgtc ggggactcct tcaaaaaaat cggtgaagag
    46381 acgttcttta ttactctggt gccggggact acaggcgagc agcaggcgct gcttcgccgg
    46441 gatcttcccc tcgccccgat ggccgacccc cggtactcgt tcttcgagac cgagtcctgc
    46501 tatggcggcg tgcaccagaa atgggtggta taccattcgg ccgcggacca cgcgaagcag
    46561 gagaaggccc tggagaagcg ccggtcaaag gaggatgaga gggtgaagaa agccctcgcc
    46621 cacctcaata accgtgaatt tgcctgcgag gcagacgctc ttgccgaggc tgagcgctgg
    46681 atccggacct acccccttta cgagtttgag tctgtttccg ttgtcacccg atggcagcag
    46741 gctcctggtc gccgggggcg gccggagaag gatggggtgc ccggggcggt gtacctgctg
    46801 gcggccagtg tcaggccgga cgaggctgcc cgggtggcag aacgggccgc tctgggcagg
    46861 tttgttcttg ccaccaatga tctcgacctc gcggcggaag accttctcgc gcggtacggg
    46921 aaccggggag tggttgagca cgggttccga ttcctgaacg attcgagttt tacgatatcg
    46981 gatctgttct tggagaacaa cgggcgtatc gaggccctgg gtatgatgat gacgctgatg
    47041 ctggcggtat actcactcgg agagtaccaa ttgaggaggc ggttggagca cctcggtaag
    47101 actgttcccg atcgagcagg gaagccgacg gcaaaaccca cgctcaagtg ggtgatgacg
    47161 ttctttgacg gagtggtgga gtatcatcgg tatgatctcg ctacgggtac ggtgcagtat
    47221 gaagggatcc tcaacatgag taaacggtgt tgggatgttg tagaggcgtt tggaccgatc
    47281 tgtaagaaat actactcgta aggctgatct tcaggctatc ggaggggcga acctcgcggg
    47341 atggaattgc ggcgaggggc gggtataacg aacgtatttt tgaaaggagg gagagatgat
    47401 cggaggcttt accgcagaaa acagaaaaaa agcaggctta ttttctaaga gagaggtgag
    47461 gaacctctct cacatcagca ccgggacgag atcggcccag gtctcgatgg cgtcgtcctg
    47521 gtaggacgat acccggacgg tctttttggt gaaggtgacg tagtagtcgt cgcctgccgg
    47581 accgtgg
//
LOCUS       DAOSII010000003        33640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_1495_length_33640_cov_50.362573, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000003 DAOSII010000000
VERSION     DAOSII010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 33640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33640
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1495_length_33640_cov_50.362573"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..468)
                     /locus_tag="PLV90_00505"
     CDS             complement(10..468)
                     /locus_tag="PLV90_00505"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF95621.1"
                     /translation="MATDIRSGARPDVRELRSAEFSAADEVWREYRGMVGDPGRDRIF
                     AVFLAGKIVSLARCRRHADGLEVDGVFTPEQYRGRGYARMAVAALVVACHNDDLYMYA
                     VENLTGLYAEFRFMSIPERDLPPGVRERYTWAAGNVEGAEVRPMYRRAGL"
     gene            complement(479..1879)
                     /locus_tag="PLV90_00510"
     CDS             complement(479..1879)
                     /locus_tag="PLV90_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF95622.1"
                     /translation="MNAGDSLTAYFSGRRNRTATVAGLTVLTLGGSLFGMILGAPANL
                     IPALLLSIPIILASYWYPRRGTLFSACIAAAYTVTAFLFSPPVAHLALSVLPRAALLI
                     LVGGVVAFLSTRLRESEQQMNQIIEFLPDATFAIDLEGRVIAWNHAIEDMTGVKKDAM
                     IARGDHEYAIPIYGERRALLADLVLQGDPGSGYPSVRREGDIFIAEARAPHLRGGRGA
                     QIRFAATTLLDHEGRVTGAIESIRDITDEVMMEAALQNTNRQLNTLSGILRTDLSNRL
                     AVLYGHLSVGAMKFDDLEVLSFIDDLKDAANGIQRQIEISREFRNIGTSPPAWIPVQQ
                     IARNAADTLSFAGVSLEIWVERLEVFADPHLGTVFTHLFENSLAPATGATRVVVTYHL
                     RPDGCAIIVEDDGTGIPDAAKEGLFVQKEKGYGRGLFLAHEILAITGIRIRETGRFGA
                     GARFELLVPPEGYRII"
     gene            complement(1922..>3004)
                     /locus_tag="PLV90_00515"
     CDS             complement(1922..>3004)
                     /locus_tag="PLV90_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOF95623.1"
                     /translation="PDIPVILVTNLFDPADVMRGLASGADSFIVKPVDPRHVRSQIEA
                     VQRAAEGSDPDGTPAGLDVSFSGNTYTITARRRTILNTLLSTYTVAVAKNTELQEAQE
                     QLHSLNDRLQEAVDDLSRSNERLAAENQERRRVEKALADANRKLQLMASITRHDLLNQ
                     LAALGGYLDLAQMMREREPANAWKQVASAGEIVNRISNTIRFTAEYQKVGSASPAWQD
                     IRSLVEKSERYVSAETVTLVNAVPAGAEVYADPLIEKVFSNLIENAVRYGKTATTVTF
                     SLRRSGDTCTILCEDDGVGVPYDEKEKIFTYAYGMNTGLGLFLAREILAITGIAIRET
                     GEPGRGARFEILCPVGVIRAPERHTG"
     assembly_gap    3005..3104
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3105..5305)
                     /locus_tag="PLV90_00520"
     CDS             complement(<3105..5305)
                     /locus_tag="PLV90_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOF95624.1"
                     /translation="MSGTDEQFRARLLDMFREEADEYLEAIAAGLIELEKTGLVPELV
                     ESVYRRIHSLKGASRAVNLREIESICQNLETIFSLVKRGEYTPGVNDFDLFHRTLAVV
                     KALLQEEQPDTSPAEIVGALRAIPGKPAGSGPGPALREPHAPEARNGNDPAGTGPDRG
                     TVRIAAHKLDRLTASADGLLTAQISIMQRIRELEEMVARFTLWQRNHSQAFNDLNLLQ
                     RRTFREEKAALPHDLVLPLQRAAEFLEYNREFVTYLQHDLATHLRAMEIDRSALKAST
                     AEISDLIHDAALLPAASMLAPFSAFVREASRAAGKSVELTIEGGEIEVDRRILDALRE
                     PVMHLIRNSIDHGIEEPPVRIAQNKPATGSVRVRIFSRSGSRVGIEVIDDGAGIDSSA
                     IRKTAVESGVITKEEDAALTDSEAIWLIFRSGMTTSRIVTDLSGRGLGLAIVEDTVSR
                     LGGRMTVSSVVGRGTTISFSVPVRMTTLRGLLVRSGRQVYVIPIQQVKQVLRVHPDSL
                     TSSRGRPTIRFSGETIEVIRLTDILGIPPSGPPPADTRPTPLVIIAYGAGQIACTVDE
                     VIRVQEIVVRPLGSQLRSVRRIEGAVILGDGSVALVLDPLELIQDALQADRPAPGPAL
                     PGEAGWRILVVEDSVTSRVLLQTILEEAGHQVETAVDGIDAFARLKQHEFDMVVSDVD
                     MPRMNGFTLTEKIRAEGRQFAGLRVALVTSLDSPEDRERGRAVGADAYIVK"
     gene            complement(5317..7698)
                     /locus_tag="PLV90_00525"
     CDS             complement(5317..7698)
                     /locus_tag="PLV90_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HOF95625.1"
                     /translation="MFTFFSDIKVGTKILVICLFLAIIPTLMLGIVAYTSSSGVINEQ
                     IETLLETQVHDARGWTNDVYKLTRNKVNSDLNVLRENFYARGTPEVINGKLTLVNAGG
                     EPYVINDNFEIVDQVQSLVGGAATVFQVYDNNAIRISTNVIGTDGSRAVGTELTQNVY
                     DVVVNQGETYYGSRDLFGKRYVTAYEPIKDSSGKIVGVLFVGTEEEETLDVVKASLKD
                     TVIGRNGYMFVIDSNGQVLVHPSLEGENWSSEAFVQEILQKKVGIIRHQVDGTDMIDA
                     YTYYEPLDWHIVSRAELSDFTGPIDTIRSTIVIVVLASIAIGVAIAILFGRSISDPLQ
                     QVVLMLKELRNGHLSARLNIRRQDEIGVMARTMDEFAGDLQTNVVGNLKKIALGEYVQ
                     EFPVADEGDEIRPALALMVQSLDHLHKETLKLTDAARAGDLSIRGDEKAFRGGYRMII
                     AGFNKTLESITEPVNEAMRLARIYASGDFTARFDEKIPVAGEFVAYRDALNTIGIELS
                     RLMKLISEELYEGVSVLSLASNEILAVTTQLAAASSQTAATVNETSDSVESVRKKTEL
                     VNQKTKGVSEKAMRALEVSKGGQRSVRETLEGINQIQRQMDIIRMNVIRLSEQSQAVG
                     EIIATVTDISEQSNLLAVNASIEAAKAGEFGRGFAVVANEIHNLAEQSKKATSNIRVI
                     LTDIQQGISSTAASTEQGTRSVADATRLTNNAQEAIEVLARSIADTSQEVIEIATSIQ
                     AQASGVDQISSAMENIRDAAQKNLETTHKAEKTAGDLHELGARLKRITQQYRV"
     gene            complement(7828..8790)
                     /locus_tag="PLV90_00530"
     CDS             complement(7828..8790)
                     /locus_tag="PLV90_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOF95626.1"
                     /translation="MAHLLPFTVAGVACALPLADVQQVTGMVELRPMTGGRDVGAVNL
                     HGRIIPVYSLRRRLGLPDRPPHPDDALIIARAGRDCAALWVDAVERVRVSTTPLPREG
                     EPSPLPGVLLTEEGMALIYDLPLFLAAEESASLPRPPDTDGDEPPREPGRDRAILADR
                     ARALMQRKEEPRETPFPELLTFRLAGREYAVGMQYIREVFILREITPVPGVPDFIAGI
                     GAVRGEIISVVDLCALFSIPKHGLTDLNRVIVLSDGAVTFGILADYITDIGTRPTDHL
                     APVDPGMTPIDRRYLVGVTDESVIVLDAAALLASPGMVIDEARK"
     gene            complement(8890..9726)
                     /locus_tag="PLV90_00535"
     CDS             complement(8890..9726)
                     /locus_tag="PLV90_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF95627.1"
                     /translation="MAWEFLTVYWREMIRFTRFRTQLLSSLLQPALWMAFFGVAMSSN
                     FNRFASSVPPPAGVVPVDYLTFMAAGVIAMTTLFTSLFGGISLLFDKNWGLMREMLAS
                     PMPRTHIMLGISLSGMTKSFIQVAIIMAFGLFLGVQFFPGYSPARVVVSILGIFLFVG
                     VFSLGFLLFSSTISMRLESPEGLQGIITLLTLPLFFVSNALYPIQAFPPLLQDLSIYN
                     PLTHLVTGVRYFALGNEFFVVGTNYSYTPADILLSFAYLVAFAAVMYAVAHRTFQKAV
                     VT"
     gene            complement(9734..10684)
                     /locus_tag="PLV90_00540"
     CDS             complement(9734..10684)
                     /locus_tag="PLV90_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF95628.1"
                     /translation="MSGDDIIEVQDLEHAFNGFQAVRGISFTVRRGEIFSFLGPNGAG
                     KSTTINILTTLLPLQQGTVRVAGHDVAREPGRVREAIGIVFQDDVLDRDLTVWETMEF
                     HGRLYSIPRDERKARIDEMLAVVELDAKRNERTKNLSGGMKRRLQIARGLLTQPEVLF
                     LDEPTQGLDTQTRMRMWDYIRGVNAAGTTVFLTTHYMEEADMLSDRISIIDHGRIIVS
                     GTPEELKNRLGEDVVYLETGDDAKVRDALQGIDEIRSITESPRGLSITISADGSHCLP
                     RIVERVHDAGIAITSVNLKKPTMDDVFVHYTGRELRDTGA"
     gene            11001..11495
                     /locus_tag="PLV90_00545"
     CDS             11001..11495
                     /locus_tag="PLV90_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95629.1"
                     /translation="MTPAARAFLCLCLLLFVLAPAADGLVITNTDLTPAEVNLLFAMR
                     EYTLTYNEYAGRLPGWVTGPFENEKVNLYVEFPDAVLVIGITFRDRKIVSFDAKGCPD
                     PIVTVTTDKATVEEVMASGRPFDTFRAAMRNGTVTVEGNTLLGMLRSGMVRFGLRVMD
                     IVGL"
     gene            complement(11522..11722)
                     /locus_tag="PLV90_00550"
     CDS             complement(11522..11722)
                     /locus_tag="PLV90_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95630.1"
                     /translation="MRIRRYKPPISVSTQPFRTVRRLQQNPTYTPLMTGPVVNAIVLA
                     ALSATSAMSGLTLGVCMRLVGL"
     gene            12049..13347
                     /locus_tag="PLV90_00555"
     CDS             12049..13347
                     /locus_tag="PLV90_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HOF95631.1"
                     /translation="MPAGVRAEWEPLRKVAVHRPGIEMFFGLLEPYAALYERAFSRYE
                     ARREHDTLVRALHEEFGVRVLRLKETILDAADSDPAVRRRLVDWAYETVTLRGGRREV
                     AEARRSMEQNADALDSLHFFTLLLLNPVIEVGRGDTGDGVDVRVPGQEPLANLYFMRD
                     QQAVTARGLVVGRPAKRQRLREPGITRFLWDILGIPVAGTVEGPGTFEGGDFMPMGTF
                     ALVGTGDRTNPAGVSQFLDFDPGFDEIGVVHQPGHPLIPGDRPDPMVDMHLDTYFNVA
                     GSGVVIGSEVLLRRARVDIYHRTGEGYVRSGETVSLDDYIRSKGFAVIDLSILEQLSY
                     AANILCIRDGSILAVEGERVMQTVLLNLERKAARDPHRYGRLLCHAEEDYRRIRAAGR
                     FFPHRKEFSRHGIDLRPLHLENLTGGYGGPHCMTCVLERG"
     gene            13352..14296
                     /gene="arcC"
                     /locus_tag="PLV90_00560"
     CDS             13352..14296
                     /gene="arcC"
                     /locus_tag="PLV90_00560"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOF95632.1"
                     /translation="MTGKTVVVALGGNAIRRHDEAGTAAEQIANVRRASRQIAEIAAD
                     GYAVAVTHGNGPQVGDILVRNELTKDTLPAMPLDVCGAESQGMIGYLLQQAMQEALRA
                     AGLDRPVATVLTQTVVDGSDPAFERPEKPIGPFYTADQAERLRKEKGWRMVQIPGRGY
                     RRVVPSPRPIALVEGEVILRLFSAGVIVIAAGGGGVPVIAGDGGAFCGVEGVVDKDRT
                     AALLAGIVGADDLLILTDVECVSLNYGGPNREDIHDMTAREAREHLAAGHFPPGSMGP
                     KVEAAIRFLEAGGKRATIASLDSAAAALAGRSGTRIRR"
     gene            14357..14695
                     /locus_tag="PLV90_00565"
     CDS             14357..14695
                     /locus_tag="PLV90_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5518 domain-containing protein"
                     /protein_id="HOF95633.1"
                     /translation="MADNFWVGVIVGWLVGLILGFFLPVVGPLVGGFVAGWIVRGGIG
                     NGAKAGLLAGIIGAIIISILILVGGTVLLGAFGLVAGVGTSIALVVAAFVYQGILSLI
                     GGAIAGAIRR"
     gene            complement(14735..15940)
                     /locus_tag="PLV90_00570"
     CDS             complement(14735..15940)
                     /locus_tag="PLV90_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF95634.1"
                     /translation="MPDSSPEVRNLSVRLILLLGVASLFGSLVSNGARSVTGPFLLAL
                     GGSAVVVGLIVGFGEFAGYALRAITGGYVDRDHGYWRVVMAGYGLLAAIPLLAAIGRW
                     ETAAVLIITERVGKAVRTPARDTILSHATTNVGRGWGFGIHKALDQVGAVIGPLVMVA
                     ALTLTGGYAEGFLLLGLPLAGVALTLSFAQSTVPRPGCLDTGERDGKTIGSPRVLSYA
                     VFIFLGMAGFVNFPILSFHLKAQAIVPDAAIPLFYASAMVVSVVVALLAGRAFDRIGT
                     RILLTIPVISTLTVLLAFSRDPGVALAGSLAWGVGIGIFETAFRASIAGLATAGRRGR
                     AYGTMSAVFGTAWFVGSAVMGILYDLSIGYIVVYVVIVEIAAVVACLWMYRSRIAVRL
                     EEGIGNLMP"
     gene            16081..17460
                     /locus_tag="PLV90_00575"
     CDS             16081..17460
                     /locus_tag="PLV90_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF95635.1"
                     /translation="MEQEEGRPYSRRLILALITVATFLNPFTGTAINLALPSIGAEFA
                     ADAATLAWVSSAYLLASVIFLLPGGRLGDSRGRVTVFMAGTIIYAAGALLTILTPTIG
                     MLLLFRFLQGIGGAMIYANSVALITYLYPPGERGYAIGLNTTAVYAGLSLGPFLGGAL
                     TQFLGWRSIFIVTALLAVPVLLYAGKFPAFLNDRHRERFDVPGLVLSSALIFCLFLGL
                     ASATTPAGMVLLAAALVLGVVFYLVERRHPNPLLPVSLLTSNRVFAASNAAALINYSA
                     TYAIAFLLSLYLQYIRGYEPVTAGTLLLVQPIVQVFVAPLSGRLADRMQPGQIASVGM
                     AISAVGLFCLSMLDATTSVGVILALLVLLGVGVGLFSSPNTTAIMASVEMRFYGSASA
                     MTAMMRSLGMMLSMGAVLVVFAVIMGSTAVTPTIFPEFLRSLHLIFLAFGILSAFGVV
                     LSLLRNRLR"
     gene            17544..19106
                     /locus_tag="PLV90_00580"
     CDS             17544..19106
                     /locus_tag="PLV90_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HOF95636.1"
                     /translation="MELAIGKAGDREIAVNAQELVTGRTCIIAQSGAGKSWGIAVLCE
                     RLLEAGIGFCLIDTEGEYFSLKDRFSLIWIGSGEGCDAEIGKADIRSLMEGAIRSRTA
                     VIFDVSEADMREEVTKFAEILYDLESRLKQPYLLIVEEADKFIPQAGTSIKKIEEISR
                     RGRKRGLGLMVATQRPSLVAKNVLSQCNSQIIGKLSIENDLKAVSLFFSSRKEVEELA
                     ELEPGEFFVMGSLSREKVRMRFGARVTKHRGVTPRLVAAPPARTEPRPTPREDPPAPP
                     PALGDAVVPVLLREEALDIAKGKRRRRFRILEPEERIVSGERVYWPLHRVDVRYVGGL
                     IRKTTKASSFVIDGLTGCIVEVDRGLKVRPGFSELLGLDEAAVRIVAGLASGGSTLVE
                     IEADTRLSPAVVKKAVKSLEAAKLVTTMKAVGDTVVYVPLLAAEVPGLATLRPGADLP
                     MEPLREEPLEAEVTESSLRIILKGLEPTAEIVGFSTIYYPVYRIRFASEQGERAIVLD
                     GCTGRELPFPDP"
     gene            19192..19350
                     /locus_tag="PLV90_00585"
     CDS             19192..19350
                     /locus_tag="PLV90_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOF95637.1"
                     /translation="MDSYRCTVCGYIYSPKIGDPTSGIKPNTAFEDLPADWKCPRCRV
                     GKNRFVKI"
     gene            19802..19960
                     /locus_tag="PLV90_00590"
     CDS             19802..19960
                     /locus_tag="PLV90_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOF95638.1"
                     /translation="MDSYRCGLCGYIYNPRDGEPARGIGPRTDFGDLPDTWACSRCGA
                     DKNLFRKV"
     assembly_gap    20031..20130
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(20197..21246)
                     /locus_tag="PLV90_00595"
     CDS             complement(20197..21246)
                     /locus_tag="PLV90_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF95639.1"
                     /translation="MLDVRALSLSLGEFSLDGVDLTVGAGEYLIILGPTGAGKTILLE
                     TIAGIYAPASGTISLDGREITRTDPKDRGIGMVYQDYMLFPHLTVGENIGFGLRQQKI
                     EPDRIKERVSETANLLGIGHLLDRTPLTLSGGEQQRAAIARALVLRPRVLLLDEPLSA
                     LDAVTRDRLRRELKVVHRAMGTTIVHITHHFEDIFALADRVAVMQDGRIVQAGTPDEV
                     FRQPATEFVAAFTGMENIFPGISRVRGSEAAIDLGAITLRTVTAVEGDVYAGIRPEEV
                     IVSREAFESSAVNAFSGTVVEVRQNGIFSRVVVDAGVPFVAVLTRQSIARLGLAEGGP
                     AHVTFKASAVHVIPR"
     gene            complement(21246..22058)
                     /locus_tag="PLV90_00600"
     CDS             complement(21246..22058)
                     /locus_tag="PLV90_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF95640.1"
                     /translation="MIRDRSALPHPSPFKALFLAVLFGLVTYMVVVIVGLVLYPPLPA
                     LIESITSPEILFAVRLSLITSAISTALCVVAAIPVAYSLARFSFPGKKAVNAVFNIPL
                     ALPPLVAGVALLIFYGPSKFGQMLTAAGFDIIYTPLGIIIAQLFVNLPYMVRVTRSAF
                     ETINPRYEHVARTLGCTEWGTFCQVTLPLARAGLIAGLVITWSKSIGEFGAVLMLAGA
                     TQMKTETLPIALFLNMSTGDLDLAIAASVILIVISVTSLAIFERYTDGRGVF"
     gene            complement(22055..22555)
                     /gene="tsaA"
                     /locus_tag="PLV90_00605"
     CDS             complement(22055..22555)
                     /gene="tsaA"
                     /locus_tag="PLV90_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOF95641.1"
                     /translation="MKENGDYPCKAIGIARTPFSDPAKTPIQSVFSTARGTVEVYPGF
                     EDGLIALAGFSHIILIYRFHRAAGEEVSERPLIDGAEPHGIFATRHFNRPNRLGISVA
                     RLIGIDGRRIVVEGIDLLDETPILDIKPYIPAFDSIPDATSGWVTPGHVEAIGRQSAA
                     FQAGRP"
     gene            complement(22552..23379)
                     /gene="modA"
                     /locus_tag="PLV90_00610"
     CDS             complement(22552..23379)
                     /gene="modA"
                     /locus_tag="PLV90_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOF95642.1"
                     /translation="MRKKIIVPAALIILAALLWTAGCTVQSPTTTDTNATPGASDVSL
                     FVYSGAGLKKPMNEIGKAFTEKTGINVEYNFAGAGALVSQMELSRKGDVFVSGGTPDY
                     RIARDKGLVGEPGYVAYHVPIIAFQKGNPKNITSVDDLARPGLKLALGGVESTAIGKA
                     GDILFEKHGIRDAVEENVVLRAPTINEVVIAMEMGTADAALLTLDSVNPETMDTIDLV
                     QEDSLTLIVPIGPTTFTTQPDAAQAFVAFVTSDEGKAFFAKYGFPTYPDPKYAGIEP"
     gene            complement(23581..24063)
                     /gene="tsaA"
                     /locus_tag="PLV90_00615"
     CDS             complement(23581..24063)
                     /gene="tsaA"
                     /locus_tag="PLV90_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOF95643.1"
                     /translation="MTGIAFTPIGTVRSPHHDPRDMPIQPVGARGIRGTIELDPTYAA
                     GLRDLEGFSRIILLYHFHRSEGYALEVVPFLDKTPRGVFATRAPRRPNAVGISMLKLI
                     AVDGATLTVEDVDILDGTPVLDIKPYVPAFDAFPEERAGWLTATSGAARTMRSDGRFC
                     "
     gene            complement(24152..24787)
                     /gene="thiE"
                     /locus_tag="PLV90_00620"
     CDS             complement(24152..24787)
                     /gene="thiE"
                     /locus_tag="PLV90_00620"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOF95644.1"
                     /translation="MGYDLYVITDEEIGRGRSHAELARRAVAGGADVIQLRDKRLSGR
                     DLFRAAVAIRGITADAGALFIVNDRLDVALAAGADGVHLGENDLPVGEARRIAPPGFV
                     IGASVSSVATAVRAAAEGADYVALSPTFATASKDDAGPGYGLTMLAEIRATISLPLVA
                     IGGITTANVAGVIAAGADGVAVISAVVGEDDVTAAARDLRARITAAKAGRR"
     gene            complement(24788..25627)
                     /gene="thiM"
                     /locus_tag="PLV90_00625"
     CDS             complement(24788..25627)
                     /gene="thiM"
                     /locus_tag="PLV90_00625"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HOF95645.1"
                     /translation="MPGSTNHGSSARFMDGTIPAALLAAVREQRPLIHHITNSVTVND
                     CANITICTGAAPVMAEAPEEVADMVTAAGALVLNIGTLSAAQVEAMLAAGRRANDLGI
                     PVILDPVGAGATAFRTGTVQRLLNALEIAVLKGNAGEIGVLAGTGGSVRGVDSGGVAG
                     DPIETARECARLTGTVVSMTGEVDVVTDGERVFLVRNGVPAMDRLSGTGCMAASVTAA
                     FVAVADDYAVAAAAALAAFGRAGERAAVSARGPYSFRTALFDELAGLSPEDLACHARI
                     EER"
     gene            25803..26756
                     /gene="mch"
                     /locus_tag="PLV90_00630"
     CDS             25803..26756
                     /gene="mch"
                     /locus_tag="PLV90_00630"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HOF95646.1"
                     /translation="MRTTLSINETAMPVVREMIGYRDELGIAVAEQENGATCIDCGVH
                     VPGGYRAGALFVETCLAGLATAAITMGRVGSVPIPFLHITVSHPALACLGSQKAGWVL
                     KAGGYSAMASGPARALAAKPKDTYKTIGYRDTSVFGILALEADTLPDEEVTGFIAEKC
                     GIDPGDLYVLVAPTASLVGSIQISGRVVTATLHKLEAKGYDVCRIRHAAGRSPIAPVK
                     RTGIAAMGATNDCNIYCGSVSLIVEGYDPVFASLPSRTSPDYGRPFSRVLEDAGYDFL
                     KVDSLLAFSPAEVTINDGTSGEVYHFGSLNPDVLLESFEIR"
     gene            complement(26784..27056)
                     /locus_tag="PLV90_00635"
     CDS             complement(26784..27056)
                     /locus_tag="PLV90_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOF95647.1"
                     /translation="MSEDRKKVPDKFPWIIPFSCEGCGDCVEACPTGSIELVDLGRKI
                     DKAWITNPETCIGCGKCAQACVVGAVQMTSYVEKAILRYREKTTPE"
     gene            complement(27063..27557)
                     /locus_tag="PLV90_00640"
     CDS             complement(27063..27557)
                     /locus_tag="PLV90_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95648.1"
                     /translation="MEVIRMTVNLTERMKAWVEAMGCHLCVATPEGVPFVVLGRYARA
                     TADGEVAFALAEDEAGVIAPVLSRNPRVAFGVSRQGGIRAAYQFKGTGELLRSGEIFD
                     AVRARAKEEKGIDAGAVLLVRLTELYCTKPGYEAGLRLDGMTPEELDAWERQRWKDVP
                     RKRN"
     gene            complement(27559..28044)
                     /locus_tag="PLV90_00645"
     CDS             complement(27559..28044)
                     /locus_tag="PLV90_00645"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOF95649.1"
                     /translation="MVKFTKEMKEALRVPGKGGSLIYLCTSSRDGNPNIAAMRFVATH
                     MDDKILIADMFLLKTKANIKENPNVAVTICHPLDEGRWWVFKGKAVDMEYGFPRDFDW
                     YGANATDILNEWGNWNKAEPPDEVPPDIVFPKAVQRGVVVVHVEEVYSIEPGHVGEKI
                     L"
     gene            complement(28061..29149)
                     /locus_tag="PLV90_00650"
     CDS             complement(28061..29149)
                     /locus_tag="PLV90_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOF95650.1"
                     /translation="MAFDLSKCNFSRCKGECLVRCPYVSYDEDEAKRAVRALIAGEPH
                     PILKDCITCAACNDFCPRGANPWDLIAERQEETGVLGIPADAKPSSDWLTKPATVIRR
                     GKPGGPLISAGGIYEVVPQAEFLTGQVFSDATIIGGGPYACGFTETHLGRASRPVKFL
                     PQFIENLARTAKEFGVDEIVFTHDACYNVATTLAMQQGVRVPFRPVHILEYIRNWLRD
                     HPDRIVNPLDIRIAVQGGCTTRYAPKGGDREIWSDWLADIFAMIGVTSVEEKRVYTGV
                     DRLCCGCGIFHTQHERAVDIQRKNVQDAADAGAEQLLFICPACISVMRATCRKMKLEP
                     IYITQLVKRALGEDLGPAGTAAFGYPVK"
     assembly_gap    29307..29406
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            29472..29663
                     /locus_tag="PLV90_00655"
     CDS             29472..29663
                     /locus_tag="PLV90_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95651.1"
                     /translation="MKKFRIELTGFLEMDTDQERDARVLAERILLEIRSVVAGHGGVQ
                     DYDIDIGSVTEKPLTKRYR"
     gene            complement(29677..31569)
                     /locus_tag="PLV90_00660"
     CDS             complement(29677..31569)
                     /locus_tag="PLV90_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF95652.1"
                     /translation="MYRCFDKLDDAVIILDRDNTVARLNRSFQEIFGIDDETVRSMET
                     EEFTTSHLARFLLEGEGADRVIEALRYRQEVSHAACRIETPDGEVRWFSFSCRMMTEE
                     PYTGMALVRLRDITSGYSEAAWNASEVEAEVGAETIFAGIGELVPYGIWICDPDGTVL
                     YISPSFLDLIGTTLEECRQSGWLGRTPLGDPAGVLADWGRCLDAGCRWDRELRVPGPG
                     GEYRTILSRGGPIRDEDGRIILWAGINLDITARREAEQITAVRAAQQAVIADLGRLAL
                     AGTDLSELMNALVRAVAGHLGVKYAKVLRYLPDEDAFLLEAAVGFNETRVGKEKVEGG
                     TDSQAGYTLLSHEPVIVEDLRTETRFTGPALLRDEGIVSGISVIIQGKRAPYGVLGAH
                     TRTHRRFTQDDINFIQAAANILAQAIERRATEEALLASEEKFRAIAQRSFDMIYTCYH
                     DSGIAYISPAVTRMLGYTPEELIGRKCRDYLSPVSLPAWEEAEKKLARGEPVEGLEVE
                     FHRKDGFVAFVELNESAILEHGKVVGVQAVGRDITERKQNEQLRRQAFSQIERNIEQF
                     AVLGDHIRQPLQVTLGRAELIDDTETAAVIREQVERINDYIRQLDRGWVESRKVREFL
                     RRHDLE"
     gene            complement(31738..31822)
                     /locus_tag="PLV90_00665"
     tRNA            complement(31738..31822)
                     /locus_tag="PLV90_00665"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31786..31788),aa:Ser,seq:cga)
     gene            31874..32077
                     /locus_tag="PLV90_00670"
     CDS             31874..32077
                     /locus_tag="PLV90_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine S protein"
                     /protein_id="HOF95653.1"
                     /translation="MLCRFTLIPDGRVLIYRGRPGETFEDALLSFELNPDTALIFSGG
                     RSLPQDKKIEEEEVEIFLASSRG"
     gene            complement(32078..>33640)
                     /gene="thsA"
                     /locus_tag="PLV90_00675"
     CDS             complement(32078..>33640)
                     /gene="thsA"
                     /locus_tag="PLV90_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit alpha"
                     /protein_id="HOF95654.1"
                     /translation="NVERTRGFEAQRSNIMAAKAIAAAVRTTLGPRGMDKMLVSSSGD
                     VVITNDGATILHEMSVQHPGAKLVVEVAETQDDEVGDGTTTATVLVGSLMEEAERLLA
                     QNVHPTVIANGYQLGMEKALAVLDDLAITIGFEDRENLIKIADTAMTGKSIEAVKEKV
                     SNIVVDAVRQVATGAGAGAYIVDEDDIKIKTQVGDTMHDAELIRGVVIDKKRVFEQMP
                     DAVTDARVALLAQPLEVTKTQVKSKIKITSSDQMKAFTEQERESLKKIADQIAASGAN
                     VVLCQKGIADAVQYYLAKSGIYAIEDVKEEDMKFAARALCGTIVNKPEELNATTLGHA
                     EAAEELPDTELTVISGCDNPKAVTILLRGTSQLLLDELERAVYDAARVVQDAIEDGRF
                     VVGGGSVETELQLRIRDYAATVGGRAQLAIEAFANAFEVIPRTLAENSGFDTIDKAVA
                     LRKAHADGAKYAGLNVYTGAIVDMREAGVIEPERVKTQAIKSATETAMLLVRVDDMMV
                     TQAGKPGMPGAQ"
BASE COUNT         5675 a        10572 c        10822 g         6271 t
ORIGIN      
        1 tacccgttcc taaagcccgg ctctccggta catcggccgg acttcagccc cctccacgtt
       61 ccctgccgcc caggtgtagc gttcgcggac gccgggcgga aggtcgcgct caggaatcga
      121 cataaacctg aactccgcgt aaagcccggt aaggttctcg accgcgtaca tgtagaggtc
      181 gtcgttgtgg caggccacga ccagggccgc gaccgccatg cgggcatacc ctctgccgcg
      241 atactgctcg ggcgtgaaga cgccgtcgac ctccaggccg tcggcgtgcc tcctgcaccg
      301 ggcgagagag acgatctttc ccgcgaggaa gacggcaaat atcctgtccc ggccgggatc
      361 gccgaccata ccgcggtact cgcgccatac ctcatctgcg gcggaaaact ctgcgctcct
      421 gagttcgcgc acatccggcc gggcccctga acgaatatcc gttgccatcc cgagcacctc
      481 agattatcct gtatccttcg ggcggcacga ggagttcgaa cctcgccccc gccccaaacc
      541 ttccggtctc gcggatcctg atcccggtga tggccaggat ctcgtgagcg aggaagagac
      601 cgcgcccgta ccccttctcc ttctggacaa agagcccctc ttttgccgcg tcgggaatcc
      661 cggttccgtc gtcctcgacg atgattgcac aaccgtccgg gcgcagatgg taggtgacga
      721 cgacccgtgt cgcgcccgtt gccggagcaa gcgagttctc aaagaggtgg gtaaagaccg
      781 tcccgagatg ggggtcggca aagacttcga gcctctccac ccatatctca agggagacgc
      841 cggcaaagga gagagtatcc gccgcgttcc tggcgatctg ctgtacgggg atccacgccg
      901 gcggcgacgt cccgatgtta cggaactcgc gggagatctc tatctgccgc tggatgccgt
      961 ttgccgcatc cttcaggtca tcgatgaacg agaggacctc gaggtcgtcg aacttcatcg
     1021 cgcccaccga gagatgcccg tacaggaccg caagcctgtt cgagaggtcg gtgcgcagga
     1081 tacccgagag ggtgttgagc tggcggttgg tgttctgcag cgcggcctcc atcatgacct
     1141 cgtcggtgat gtcccggatg gactcgatgg ctccggtgac cctcccctcg tggtcgagaa
     1201 gcgtggtcgc cgcaaacctg atctgcgctc cccggccgcc ccggagatgc ggggcgcggg
     1261 cctccgcaat gaagatatcc ccctccctcc tgaccgaggg atacccgctc ccggggtcgc
     1321 cctggaggac caggtcggcg agcagcgccc gccgttcacc gtagatgggt atggcatact
     1381 cgtgatcgcc gcgggcgatc atggcatcct tcttcacccc ggtcatatcc tcgatcgcgt
     1441 ggttccaggc aatgactctt ccttcgagat cgatggcgaa tgtcgcgtcg ggcaggaact
     1501 cgatgatctg gttcatctgc tgctcggact cccggagccg cgtcgagagg aacgcgacca
     1561 cgccgccgac caggataagg agggccgcac gggggaggac gctgagagca aggtgggcga
     1621 cgggcgggga gaagaggaag gccgtgacgg tgtaggcggc cgcgatgcag gcggagaaga
     1681 gggtgccgcg ccgcggatac cagtaactcg cgagtatgat cggtatgctg agcaggaggg
     1741 cagggatgag gtttgcggga gctccgagga tcatgccgaa caggctcccg ccgagggtca
     1801 gaacggtcag tccggctacg gtcgcggttc ggttccgcct gccggagaag tatgcggtga
     1861 gagagtctcc ggcgttcatg aggatacctg tatattccgg gcgcgagtcc ctggccgata
     1921 ctcacccggt atgccgttcg ggagcacgga tcacccccac cggacagagg atctcgaacc
     1981 gggcgcccct ccccggctct cccgtctccc tgatggcgat gccggtgatg gcgaggatct
     2041 cgcgtgcgag gaagaggccg agcccggtgt tcatgccgta tgcgtaggtg aatatcttct
     2101 ctttctcgtc atacggaaca cccaccccgt catcctcgca gaggatggta caggtatccc
     2161 cactccttct caggctgaat gtgacggtgg tcgccgtctt cccgtagcgc accgcgttct
     2221 cgatgaggtt tgagaagacc ttctcgatga gcggatcggc gtatacctcc gcgcccgccg
     2281 gcacggcgtt cacgagggtc acggtttccg ccgagacata ccgctccgac ttctccacga
     2341 gcgaccggat atcctgccag gcgggggacg ccgacccgac cttctggtac tcggcggtaa
     2401 atcggatggt gttgctgatc cggttcacga tctcgccggc gctcgcgacc tgcttccatg
     2461 cgtttgccgg ctctctctcg cgcatcatct gggccagatc gagataccca ccgagcgccg
     2521 caagctggtt taagaggtcg tggcgggtga tgctcgccat gagctggagt ttccggtttg
     2581 catccgcaag cgccttctcc acgcgccgcc gttcctggtt ctccgccgca agcctctcgt
     2641 tgctgcgcga gaggtcatcg acggcctcct ggagccggtc gtttaaggag tggagttgtt
     2701 cctgcgcctc ctggagttcg gtgttctttg ccaccgcgac ggtgtaggtc gagaggaggg
     2761 tgtttaagat cgtgcgcctc cgggcggtga tggtataggt gttgccggag aaggagacat
     2821 cgagcccggc aggggtgccg tccgggtcgg acccctccgc cgccctctgg acggcctcga
     2881 tctgggaacg gacgtgccgg ggatcgacgg gtttgactat gaagctgtcg gccccggatg
     2941 caagcccccg catcacgtcg gcggggtcga agaggttggt gaccagtatg accgggatat
     3001 ccggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnctcttg acgatatagg
     3121 catccgcccc gaccgccctc ccccgctccc ggtcttcggg tgaatcgagc gaggtgacca
     3181 gcgccacccg gagtccggca aactgccggc cttctgcgcg tatcttctcg gtgagcgtaa
     3241 aaccgttcat ccggggcata tctacatccg agacgaccat atcaaactca tgctgcttaa
     3301 gccttgcgaa tgcatctatg ccgtcgacgg cggtctctac ctgatgcccg gcctcctcga
     3361 ggattgtctg cagcaggacc ctcgatgtga ccgaatcctc gacgacgagt atccgccacc
     3421 ccgcctctcc cgggagtgcg ggcccgggtg ccggcctatc cgcctgcagc gcatcctgga
     3481 tcagttcgag agggtcgaga acgagcgcca cgctgccgtc tccgaggata acggccccct
     3541 cgatcctccg gacggagcgg agctggctcc cgagcggccg gacgacgatc tcctgcaccc
     3601 ggatcacctc gtcgaccgtg caggcgatct gccccgcccc gtaggcgatg atgacgaggg
     3661 gggttggccg cgtgtccgcg ggaggggggc cggacggagg gatgcccagg atatcggtga
     3721 gcctgatgac ctcgatcgtc tccccggaga accggatcgt cggccggccc cgcgaggaag
     3781 tcagggagtc ggggtgcacc cggaggacct gtttcacctg ctgtatgggg atgacgtaga
     3841 cctgcctccc ggaacggacg agcaggccgc ggagggtggt catcctcacc gggacggaga
     3901 aactgatggt ggttcccctg ccgacgaccg aggataccgt catacgcccc ccgaggcggg
     3961 atacggtatc ctcgacgatt gcaagtccaa ggcccctccc cgagaggtcg gtgacgatcc
     4021 ggctcgtcgt catccccgac cggaagatga gccagatcgc ctcgctatcc gtgagggccg
     4081 catcctcttc cttcgtgatc accccgctct cgacggcggt cttccggatc gcgctgctgt
     4141 cgatgccggc cccgtcgtcg atcacctcga tgccgacccg gctgccggag cgggagaaga
     4201 tccgaacccg cacggaaccg gtggcaggtt tgttctgagc tatcctgacc ggcggctcct
     4261 cgataccgtg atcgatgctg ttgcggatca ggtgcatgac gggttctctc aatgcatcga
     4321 ggatacgccg gtccacctcg atctcccctc cctcgatcgt gagctcgacg gactttcccg
     4381 ctgcgcggga ggcctccctc acgaacgcgg agaacggcgc gagcatgctt gccgccggaa
     4441 gcagcgcggc atcgtggatc aggtctgaga tctcggcggt gctcgccttg agcgccgacc
     4501 ggtcgatctc catggcccgc aggtgcgtgg caaggtcgtg ctgcaggtag gtcacgaact
     4561 cccggttgta ctctaaaaac tcggccgccc tctggagcgg caggaccagg tcgtggggga
     4621 gcgccgcctt ctcctcgcga aaggtcctcc tctggagcag gttcaggtcg ttgaacgcct
     4681 gggagtggtt ccgctgccag agggtaaagc gggcgaccat ctcctcgagc tcgcgtatcc
     4741 gctgcatgat ggatatctgc gccgtgagaa ggccatcggc gctggcggta agccggtcga
     4801 gtttgtgggc cgcgatccgc accgtgcccc gatccggccc tgttccggcc ggatcgttgc
     4861 cgttgcgagc ctcgggagcg tggggttccc ggagggccgg acccgggccg ctcccggcgg
     4921 gtttgccggg gatggcccgg agcgctccga cgatctctgc cggcgatgtg tcgggctgct
     4981 cttcctggag aagcgccttc acgaccgcga gcgtccggtg gaagaggtcg aagtcgttca
     5041 cgccgggggt gtactcgccc cgtttcacga gagagaagat ggtctcgagg ttctggcaga
     5101 tggactcgat ctcgcggagg ttgaccgccc gcgaggcgcc tttcaggctg tggatcctcc
     5161 ggtagaccga ctcgaccagt tcgggcacga gcccggtctt ctcgagctcg atcaaccctg
     5221 cggcgatcgc ttcaaggtac tcgtccgcct cctcgcggaa catatcgagg aggcgggcgc
     5281 ggaattgttc gtccgtcccg ctcatggcac gtcgtgtcag acccggtact gctgggtgat
     5341 cctcttcagg cgagcgccga gctcgtgcag gtccccggcg gtcttctccg ccttgtgggt
     5401 ggtctcgagg ttcttctgtg ccgcatcgcg gatgttctcc atcgcactgg agatctggtc
     5461 gactcctgaa gcctgcgcct ggatcgacgt ggcgatctcg atgacctcct gcgacgtatc
     5521 cgctatcgac cgggcgagca cctcgatcgc ctcctgggcg ttgttcgtca accgggtcgc
     5581 atccgcaacc gaccgggtcc cctgctcggt cgaggccgcg gtcgaggata tgccctgctg
     5641 aatatcggtc agaatgaccc tgatgttcga tgtcgccttc ttcgactgtt cggcgaggtt
     5701 atggatctcg tttgcgacga ccgcaaaccc tctcccgaac tcgccggcct ttgcagcctc
     5761 gatggaagcg ttcaccgcga ggaggttgga ctgctcggat atatcggtca cggtggcgat
     5821 gatctcgccg acggcctggc tctgctccga gagcctgatg acgttcatcc ggatgatgtc
     5881 catctgccgc tggatctggt tgataccctc cagggtctcg cgcaccgatc gctggccgcc
     5941 tttcgagacc tcgagcgccc gcatcgcctt ctcggagaca cctttcgtct tctggttcac
     6001 gagttcggtc ttcttcctga cgctctcgac cgagtcggac gtctcgttca ccgttgcggc
     6061 ggtctgcgaa ctggccgcgg caagctgggt cgtgaccgca aggatctcgt ttgatgcaag
     6121 cgagaggacc gagactcctt catagagttc ctcgctgatc agcttcatca gacgcgagag
     6181 ctcgatgccg atcgtgttga gagcgtcgcg gtaggcgacg aactctccgg caaccggtat
     6241 cttttcatcg aaccgggcgg tgaagtcgcc ggatgcgtag atcctcgcga gccgcatcgc
     6301 ctcgttcacc ggttcggtta tcgattcgag tgtcttgttg aacccggcga tgatcatccg
     6361 gtatcccccg cggaacgctt tctcgtcccc gcggatggag aggtcgcccg cacgggcggc
     6421 atccgtgagt ttgagcgtct ccttgtggag atggtcgagg gactggacca tcagcgcaag
     6481 tgcggggcgg atctcgtcgc cctcatcggc gaccgggaac tcctggacgt actcgccgag
     6541 ggcgatcttt tttaagttcc cgacgacgtt cgtctggagg tcgccggcaa actcatccat
     6601 cgtccttgcc atgacaccga tctcgtcctg ccgccggatg ttgagccggg cggagagatg
     6661 gccgttcctg agttctttga gcatcagcac gacctgctgg agcggatcgg atatcgagcg
     6721 gccgaagagg atggctatcg caacaccgat ggcgatggat gcgaggacga cgatgacgat
     6781 cgtgctccgg atggtgtcga tcggcccggt gaagtcggag agttcggcac gcgagacgat
     6841 gtgccagtcg agcggttcgt agtaggtgta ggcgtcgatc atatccgtcc cgtcgacctg
     6901 gtggcggatg atgccgacct tcttctggag tatctcctgg acgaaggcct cgctcgacca
     6961 gttctctccc tcgagggagg ggtgcacgag cacctggccg ttgctgtcga tgacgaacat
     7021 gtagccgttc ctgccgatga ccgtatcctt gaggcttgcc ttgacgacgt cgagggtctc
     7081 ttcctcctcc gtcccgacga agaggacccc gacgatcttc ccgctgctgt cttttatcgg
     7141 ttcgtatgcg gtgacgtagc gtttgccaaa gaggtctctg ctcccgtagt aggtctcccc
     7201 ctggttcacc acgacatcgt agacgttctg ggtcagctcg gtcccgaccg ccctcgaccc
     7261 gtcggtcccg atgacgttcg tggatatgcg gatggcgttg ttgtcataaa cctggaagac
     7321 ggtcgccgca ccgccgacca gggactgcac ctggtcgacg atctcgaagt tatcgttgat
     7381 gacgtagggc tcgccgccgg cgttcacgag cgtcagtttt ccgttgatga cctcgggagt
     7441 gccccgggcg tagaagttct cacggagaac gtttaagtcg ctgttcacct tattgcgcgt
     7501 gagtttgtag acgtcgttcg tccaccctct cgcatcgtgc acctgcgtct caagcagcgt
     7561 ctcgatctgc tcgttgatca caccgctcga gctggtgtac gccactatgc cgagcatgag
     7621 cgtcgggatg atcgcgagaa acaggcagat gacgaggatc ttcgtcccga cctttatatc
     7681 cgaaaaaaac gtgaacatgg cttgcctccg ttccactcgt tcctgtacga cgtactagaa
     7741 taatgcacac ccggggttaa ataacgatcc atcccgggaa aagcgattcc tggcgggttt
     7801 tcggcataac ggccggttaa cgcggcgtta tttcctggcc tcgtctatca ccatcccggg
     7861 agaggcaagg agtgccgctg cgtcgaggac gatcaccgat tcgtctgtga ctcccaccag
     7921 gtatcgccgg tcgataggcg tcatgccggg gtctacggga gcgaggtggt cggtcggccg
     7981 ggtgccgatg tctgttatgt aatcggcgag gatcccgaag gttactgctc catcggagag
     8041 gacgatgacc cggttgaggt ccgtcaaccc gtgtttcggg atcgagaaga gtgcgcagag
     8101 atcgacgacg gatatgatct ctcccctgac ggcaccgata ccggctatga aatcgggcac
     8161 cccggggacc ggagtgatct cgcggaggat gaagacctcg cggatatact gcatcccgac
     8221 ggcgtactcg cgaccggcga gacggaacgt gaggagctcc gggaacggcg tctcacgcgg
     8281 ctcttccttt cgctgcatga gtgccctggc ccggtcggcg aggatcgccc tatccctccc
     8341 gggctcgcgg ggcggctcgt ctccatccgt atccgggggg cgggggaggg atgcgctctc
     8401 ttccgcggca aggaagagag ggaggtcgta tatgagggcc atcccttctt cggtcagcag
     8461 cacgccgggg agaggggacg gttctccctc tcgcgggagg ggggtcgtgc tcacccgcac
     8521 cctctccacg gcgtccaccc agagggccgc gcagtcacgg cccgcgcgag cgatgatcag
     8581 ggcatcgtcg gggtgcggcg ggcggtcagg gagcccgagg cgcctccgga gcgagtagac
     8641 ggggatgatc ctgccgtgca ggttcacggc ccccacatct cttccaccgg tcatcggccg
     8701 gagctccacc ataccggtaa cctgctgaac atcggcgaga gggagggcgc aggccacccc
     8761 cgcaacggtg aaggggagaa ggtgggccat cgtggatact cgaaggttgg tccggggcgg
     8821 ataaaaggtt acgggcagat ctccgatatt cccatgatcg tcgggatgag tctctcctct
     8881 tacgggtgtt cacgtcacca cggccttctg aaacgtccgg tgggcgacgg cgtacatcac
     8941 cgctgcaaaa gcgacgaggt aggcaaacga gaggaggata tcggccggag tgtatgagta
     9001 gttcgtcccg acgacgaaga actcgttccc gagggcgaag tagcggaccc cggtgaccag
     9061 gtgggtcagg ggattgtaga tcgagagatc ctggaggagc ggcgggaatg cctggatcgg
     9121 gtagagggcg ttcgagacga agaagagcgg cagggtgagg agcgtgatga tcccctgcaa
     9181 tccttcgggg ctctcgaggc gcatcgatat ggtcgaggag aagaggagga acccgagcga
     9241 gaagacgccg acgaagagaa agataccgag gatcgagacg acgacccggg cgggcgagta
     9301 tcccgggaag aactgcaccc caaggaagag cccgaacgcc atgatgatgg cgacctggat
     9361 gaacgacttc gtcatacccg agaggctgat cccgagcatg atgtgggtcc ggggcatggg
     9421 gcttgccagc atctcccgca tcagccccca gttcttatcg aagaggaggc tgatccctcc
     9481 aaagaggctc gtaaagaggg tcgtcatcgc aatcaccccg gcggccatga aggtgaggta
     9541 gtcgaccggg acgacgcccg ccgggggcgg gacggatgat gcgaaccggt tgaagttcga
     9601 ggacatcgca acaccgaaga acgccatcca gagcgccggc tgcaggagcg acgagaggag
     9661 ctgcgtccgg aaccgggtga accggatcat ctcccgccag tagacggtga gaaactccca
     9721 ggccattccc tgttcacgcc cccgtatccc gcagttccct gccggtgtag tggacgaaga
     9781 cgtcgtccat cgtcggtttc ttgaggttca cgctcgtgat agcgatcccg gcatcgtgca
     9841 cccgttcgac gatccgcggg agacagtggc tcccgtcggc cgatatcgtg atcgagaggc
     9901 cgcggggcga ctccgtgatc gaccggatct catcgattcc ctggagggca tcccgaactt
     9961 ttgcatcgtc gccggtctcg aggtaaacga cgtcctcgcc gagcctgttc ttgagttctt
    10021 caggggtgcc ggagacgatg atcctgccgt gatcgatgat gctgatccgg tccgagagca
    10081 tatcggcctc ctccatgtag tgcgtcgtca ggaagaccgt cgtcccggcc gcgttcaccc
    10141 ctctgatgta gtcccacatc cgcatccgcg tctgggtgtc gagcccctgc gtcggttcgt
    10201 cgaggaagag gacctccggt tgggtcagga gaccccgcgc gatctggagg cgccgtttca
    10261 taccgccgga gaggtttttc gtccgctcgt tgcgtttcgc gtcgagctcg acgaccgcga
    10321 gcatctcgtc tatccgtgct ttgcgctcgt cgcgggggat cgagtagagt cgcccgtgaa
    10381 actccatcgt ctcccagacg gtgaggtcgc ggtcgaggac atcgtcctga aagacgatcc
    10441 cgatcgcctc cctgacccgc ccgggctccc gggcgacgtc gtggccggcc acccgcaccg
    10501 tgccctgctg gagggggagg agcgttgtca gtatgttgat ggtggtgctc ttccccgcgc
    10561 cgttcgggcc gaggaacgag aagatctcgc ctctccggac ggtgaaactg atgccgcgta
    10621 ccgcctgaaa gccgttgaac gcatgttcga ggtcctgcac ctcgatgata tcatccccgc
    10681 tcaatcgctc accgcccgcg cccacacctc cgcgcccaca cctccatgta cggtgctctc
    10741 atatctcctt ttctcttccc cggcaccatg cgatctccct ctccccaccg cgaaccccgg
    10801 cgcgatcccg ggcgggaaaa ccgcattggc aactgcccac agaccggtcc ggataggtat
    10861 tctcaaatac caaactcccc catttctctt caggaagata tgtgcggcta ctggttgccc
    10921 acccctgccg gcgatgcctg ctcccggtcc cggcggagca ccggcgtgcg ggagttcgag
    10981 gagcgggcgg gagaacgaca gtgaccccgg ctgcccgggc tttcctctgc ctctgcctcc
    11041 tcctcttcgt gcttgcgccg gcggcagacg ggctcgtcat caccaacacc gacctgacgc
    11101 cggcggaagt gaacctcctc tttgccatgc gggagtacac cctgacctac aacgagtatg
    11161 ccggtcggct cccgggatgg gtgaccgggc cgtttgagaa cgagaaggtc aacctctacg
    11221 tcgagttccc cgacgctgtc ctggtcatcg ggatcacctt tcgcgacaga aagatcgtct
    11281 cgttcgacgc aaaaggctgc cccgacccga tcgtcacggt gacgaccgat aaggcgacgg
    11341 tggaggaggt catggcctcc ggccggccgt ttgacacgtt ccgggcggcg atgcgaaacg
    11401 gcaccgtcac ggtcgagggg aacaccctgc tcggtatgct gcggtcgggt atggtccggt
    11461 tcgggctccg ggtgatggat atcgtcgggt tgtgagggga aaaaagagcc ggcggcgggt
    11521 ctcacaggcc cacaaggcgc atgcagaccc caagagtcag ccctgacatg gccgatgtcg
    11581 ccgagagggc ggcgagcacg atggcgttca ccaccgggcc ggtcatgagc ggggtgtagg
    11641 tcgggttctg ttgtagccgc cgcactgtcc ggaacggttg cgtgctcacc gatatggggg
    11701 gtttgtagcg tcgtatcctc atggaaacac ctcctggtat cgtgtgaata taccttgtga
    11761 gtcctttgtt tccccgaaaa gataatcgtt gcggttccgc ccgccggata tacccgggcg
    11821 ggtggaagag ccctgtcacg acagatttat ccgatctccg gcgagagaat ccctacagat
    11881 ggatcggtat acagaactcc ttttctacgg caaacacgtt tctcttctct cttctggccc
    11941 ttatttcacc gctgatgggt gggtgagcca ggtatccgcc gcggcggcct ccgcccgggt
    12001 gatgatcgcg aatccctgta tgggcccccg gtggaggcgg tgaacgcgat gccggcggga
    12061 gtgcgggcgg agtgggagcc gctccggaag gtggccgttc accggccggg gatcgagatg
    12121 ttcttcgggc tgctggagcc gtatgcggcg ctctacgaac gggcgttcag ccgctacgag
    12181 gcgcgccggg agcacgatac ccttgtgcgt gccctccacg aggagttcgg tgtccgggtg
    12241 ctgcggctca aggagacgat cctcgatgcc gccgacagcg acccggcagt gcgccggcga
    12301 ctcgtcgatt gggcgtacga gacggtaacc ctccggggcg gccggcgcga ggtagccgag
    12361 gcgcgccgga gcatggagca gaacgccgat gctctcgact cgttgcattt cttcaccctc
    12421 ctcctcttaa acccggtcat cgaggtgggg cggggggata ccggcgacgg ggtggacgtc
    12481 cgggtgccgg ggcaggagcc actcgcaaac ctctacttca tgcgcgacca gcaggcggtg
    12541 accgcccgcg gcctcgtcgt cggccggccg gcaaaacggc agcggctgcg ggagcccggg
    12601 atcacccggt tcctctggga tatactcggc atcccggtcg ccggaacggt tgaggggccc
    12661 gggacgtttg agggcggcga cttcatgccg atgggaacgt tcgccctcgt cggcaccgga
    12721 gaccggacga acccggccgg ggtcagccag ttcctcgact tcgacccggg gtttgacgag
    12781 atcggcgtcg tccaccagcc gggccacccc ctcataccgg gcgaccggcc cgaccccatg
    12841 gtcgacatgc accttgatac ctacttcaac gtcgcgggca gcggtgtcgt gatcggctcg
    12901 gaagtcctcc tccggcgggc cagggtcgat atctaccaca ggacgggcga gggttacgtg
    12961 cggtcgggcg agaccgtgag cctcgacgac tacatccgtt cgaaagggtt tgcggtgatc
    13021 gacctctcga tactcgagca gctctcgtat gccgcaaaca tactctgcat ccgcgacggg
    13081 agcatccttg cggtcgaggg ggagcgggtg atgcagacgg ttctcctgaa cctggagcgg
    13141 aaagccgcgc gcgatcccca ccgctacggg cggctcctct gccatgccga ggaggattat
    13201 cgccggatcc gggccgccgg ccggttcttc ccgcacagga aggagttctc ccggcacggc
    13261 atcgatctcc ggccgctcca ccttgagaac ctgacggggg gttacggcgg cccgcactgt
    13321 atgacctgtg tgctggagcg ggggtaactg aatgacagga aagactgttg ttgtcgcgct
    13381 cggtgggaac gccatccggc ggcacgacga ggcagggacg gccgcagaac agattgcgaa
    13441 cgtccggcgg gcatcacgcc agatagcaga gattgcagcc gacggctacg ccgtcgccgt
    13501 cacccacggg aacggcccgc aagtggggga tatactcgtc cgaaacgagc tcacgaagga
    13561 taccctcccg gcgatgccgc tcgatgtctg cggggcggag agccagggga tgatcggcta
    13621 cctcctccag caggcgatgc aggaggccct ccgggcagcc gggctcgacc gtccggtcgc
    13681 gacggtgctc acccagaccg tggtggacgg cagcgatccg gcgtttgaaa gaccggaaaa
    13741 acccatcggc ccgttctata cggccgatca ggcggaacgc cttcggaagg agaagggctg
    13801 gcggatggtg cagataccgg gaaggggcta ccggcgggtg gttccctcgc cgcgtcccat
    13861 cgccctcgtc gaaggagagg tgatcctccg gctcttctcg gccggggtga tcgtgatcgc
    13921 cgcgggcggc gggggtgtcc cggtgatagc gggcgacggg ggcgcctttt gcggcgtcga
    13981 gggagtggtc gacaaagacc gcacggcggc actccttgcc gggatcgtcg gcgccgacga
    14041 tcttttgatc ctgaccgacg tcgagtgcgt ctccttaaac tatggggggc cgaatcgaga
    14101 ggatatccac gatatgacgg ctcgcgaggc gcgggagcac ctcgcggcag ggcacttccc
    14161 gcccgggagc atgggcccga aggtcgaggc ggcgatccgg ttccttgagg ccggagggaa
    14221 gcgggccacc atcgcatcgc tcgattctgc cgctgccgcc cttgccgggc ggtcgggaac
    14281 ccggatccgg cggtgaccgg cgccacaccc caactcttat atactatata tccggaatcc
    14341 acggcggtga atgggtatgg cagataactt ctgggtaggg gtcatcgtcg ggtggctcgt
    14401 ggggctgatc ctcggcttct tcctgccggt cgtcggcccg ctggtcggcg ggttcgtcgc
    14461 cggctggatc gtccggggcg ggattggaaa cggtgcaaaa gcaggactgc tcgccggcat
    14521 catcggtgcc atcatcatat cgatcctgat cctcgtcggc ggcacggtgc tcctcggggc
    14581 gttcgggctt gttgccggcg tcgggacatc gattgctctc gttgttgcgg cgttcgtcta
    14641 ccaggggatc ctctccctga tcggcggcgc catcgccggc gcgatccggc ggtaacgcgg
    14701 ggaggaccat cctccccggc aggagaggcg ggaatcacgg catgagattc cctatcccct
    14761 cctctaaccg caccgcgatg cgggagcggt acatccagag gcaggcaacc actgctgcaa
    14821 tctcaacgat gacgacgtag acgacgatat acccgatcga gagatcgtag agaatgccca
    14881 tcacggcgct cccgacgaac cacgccgtcc cgaagacagc gctcatggtg ccgtaggccc
    14941 tccccctccg gccagccgtc gccaacccgg cgatcgatgc ccgaaaagcg gtctcgaata
    15001 taccgatccc gacgccccag gcaagcgatc ccgcgagggc cacacccggg tcgcgtgaga
    15061 aggcaaggag gaccgtcagc gtgctgatca ccgggatggt gaggaggatg cgggtgccga
    15121 tgcggtcgaa ggcccggccg gcaagcagcg ccacgacgac cgagaccacc atcgcggatg
    15181 cgtagaagag ggggatggcg gcgtcgggga cgatcgcctg cgcctttaag tggaacgaca
    15241 ggatcgggaa gttcacgaac ccggccatgc cgaggaatat gaacacggca tacgagagga
    15301 cccggggaga tccgatggtt ttaccgtctc tttcgccggt atccaggcat cccggccttg
    15361 gtaccgtcga ctgcgcaaag gagagggtca gtgcaacccc ggcgagagga agaccgagca
    15421 ggagaaaccc ctccgcgtaa ccgccggtga gggtgagggc ggcgaccatg acgaggggcc
    15481 ctatcaccgc gccgacctgg tcgagcgcct tgtggattcc aaacccccat ccccgcccaa
    15541 cgttcgtcgt cgcgtgggag aggatcgtgt cccgtgccgg cgtcctgacg gcttttccga
    15601 cccgttcagt gatgatgagg acggccgcgg tctcccaccg cccgatggcg gcaaggagcg
    15661 gtatggcggc aaggaggccg tagccggcca tcaccaccct ccagtagcca tggtcccggt
    15721 cgacgtagcc gccggtgatc gcccggaggg cgtaccctgc aaactcgccg aacccgacga
    15781 tcaggccgac gacgactgcg ctccccccga gggcgaggag gaagggtccg gtgacggatc
    15841 gggcgccgtt tgagaccagg ctcccaaaga ggcttgccac gccgagcagc aggatgagcc
    15901 ggaccgagag attgcggacc tccggggaag agtcaggcat atgcgcgggg gtcttctgga
    15961 gccgctcaca ttaattgtag tgtatgggca ggcatcagtg ctcttattct cgcttgccgc
    16021 acatactcct gtaccgaccc gggcgggcct cggctctccg ggaagcgcag gtgacgacga
    16081 atggagcagg aagaggggcg gccgtactcg agaaggctca tcctggcact catcacggtc
    16141 gccacgttct tgaatccgtt cacgggcacc gcgatcaacc tcgccctgcc ctccatcggg
    16201 gccgagttcg ctgccgatgc cgccacgctc gcctgggtat cgagcgccta cctccttgcg
    16261 tcggtcatct tcctcctccc cggggggagg cttggcgact cccggggaag ggtcaccgtc
    16321 ttcatggccg ggaccatcat ctacgccgcc ggggccctcc tcaccatcct cacgcccacg
    16381 atcggcatgc tcctcctctt ccggttcctg caggggatag gcggcgccat gatctacgca
    16441 aacagcgtgg ccctgatcac ctacctctat ccccccggcg agcggggcta cgctatcggg
    16501 ctcaacacca ccgccgtcta cgccgggctc tccctcgggc cgttcctcgg cggcgccctg
    16561 acccagttcc tcggctggcg gagcatattc atcgtgaccg cgctccttgc cgtcccggtc
    16621 ctcctctacg ccggaaagtt tccggcgttc ttgaatgacc ggcaccggga gcgcttcgac
    16681 gtcccgggcc tcgtcctctc ctccgccctg atcttctgcc tcttcctggg gctggcctcg
    16741 gcgaccaccc cggccggcat ggtgctcctc gcggcggcgc tcgtgctcgg ggtggtcttc
    16801 tacctggtgg agcggcggca cccaaacccc ctcctccctg tctcgctgct tacatcgaac
    16861 cgggtctttg ccgcatcgaa cgccgctgcc ctgatcaatt acagcgccac ctacgcgatc
    16921 gcgttcctcc tctccctcta cctccagtac ataagaggct acgaacccgt caccgccggc
    16981 actctcctcc tcgtacagcc gatcgtccag gtcttcgtcg cccctctatc gggccgtctc
    17041 gccgaccgga tgcagcccgg acagatcgcc tcggtgggga tggcgatatc ggccgtgggc
    17101 ctcttctgcc tctcgatgct cgatgcgacg acatcggtcg gcgtgatcct cgccctcctg
    17161 gtcctcctgg gcgtaggggt cggcctcttc tcgtctccga acaccaccgc catcatggcc
    17221 tctgtggaga tgagatttta cgggagcgca tcggccatga cggcgatgat gcgctcgctc
    17281 ggcatgatgc tgagcatggg ggcggtcctc gtggtctttg cggtcatcat ggggtcgacc
    17341 gccgtcacac cgactatatt ccctgagttt ctgaggagcc tgcacctgat ctttctcgcg
    17401 ttcggcatcc tctcggcttt cggtgtcgtc ctatcgctcc tccggaacag attgcggtaa
    17461 atatccgcct gatctcccta tatctctctt cgaaaccgat gggaaacgtt aaacctcggg
    17521 gcatcctgtg atcatctgat cagatggagc tcgccatcgg aaaggccggc gaccgtgaga
    17581 tcgccgtcaa tgcacaggag ctggtcacgg gacggacctg catcatcgcg cagtcggggg
    17641 ccgggaagag ctgggggatt gcggtcctct gcgaacggct ccttgaggcg gggatcgggt
    17701 tctgcctcat cgatacggag ggcgagtact tctcgctcaa ggaccggttc tccctcatat
    17761 ggatcggctc gggcgagggc tgcgacgcgg agatcgggaa ggcggatatc cggagcctga
    17821 tggagggtgc gatccgctcg cggacggcgg tgatcttcga cgtatcggag gccgatatgc
    17881 gcgaggaggt gacgaagttt gccgagatcc tctacgacct ggagagccgg ttaaagcagc
    17941 cctacctcct gatcgtggag gaggcggaca agttcatccc ccaggcgggg acatcgatca
    18001 agaagatcga ggagatatcc cgccgggggc gcaagcgcgg cctcgggttg atggtcgcga
    18061 cgcagcggcc gtcgctcgtc gcaaagaacg tcctctcgca gtgcaacagc cagatcatcg
    18121 ggaagctctc gatcgagaac gatctgaaag cggtcagcct ctttttttcg tcccgcaaag
    18181 aggtcgaaga gctcgcggag cttgagcccg gggagttctt cgtgatgggc tctctctccc
    18241 gggagaaggt gcggatgcgg tttggggccc gggtgacgaa gcaccggggg gtgacgcccc
    18301 gtctcgtggc cgcaccgccg gcccggaccg aaccgcgccc caccccccgg gaggacccgc
    18361 cggccccgcc gccggccctc ggggatgccg tcgtcccggt cctgctccgc gaggaggcgc
    18421 tcgatatagc gaaagggaag cgcaggcgcc ggttccggat acttgaaccc gaagagcgga
    18481 tcgtctccgg ggagcgggtc tactggccgc ttcaccgggt ggatgtccgc tacgtcggcg
    18541 ggctcatacg aaaaacgacg aaggcgtcgt cgttcgtcat cgatgggctt accggctgca
    18601 tcgtcgaggt ggaccggggg ttgaaggtcc ggccggggtt ctcggaactc ctggggctcg
    18661 atgaggctgc ggtgaggatc gttgccgggc ttgcgagcgg cgggtcgacg ctggtcgaga
    18721 tcgaggccga tacccgcctc tccccggcgg tggtgaagaa ggccgtgaag agcctcgagg
    18781 cggcaaaact cgtcaccacg atgaaggcgg tgggggatac ggtggtctac gtccccctgc
    18841 tcgcggcgga ggttcccggg ctcgccaccc tccggcccgg ggcggacctc ccgatggagc
    18901 cgctccgcga ggagcccctt gaggcggagg tcacggagtc ctcgctccgg atcatattga
    18961 aagggcttga gccgacggcg gagatcgtcg ggttctccac catctactac ccggtctacc
    19021 gaatccggtt tgcgtcggag cagggggagc gcgcgatcgt cctcgatggc tgcaccgggc
    19081 gagaactccc cttcccggat ccctgaccgg agtgcttatc cctgcccggg ggtaaatgga
    19141 gatcgtataa ccctgccggc ctcacggccg ggggaagaag gagtaccagt tatggattcg
    19201 taccgatgca ctgtctgcgg ctacatctac agccccaaga tcggggaccc tacgagcggc
    19261 atcaagccga atacagcctt cgaagatctt ccggccgact ggaaatgccc ccggtgccgt
    19321 gtgggaaaga accggttcgt gaagatctga cgggagaccc taccactctt tttatcgagg
    19381 gcgccggcca tcggcccggg atactgattc ctcatcaagg cttcacgtga ggccgcctcg
    19441 ttgtgtatac ccctgtcagc tcatgcgttg ccgcgaagtt cgggtgctgc cagccccgtg
    19501 ttaaggcacc gcgagaccag gtcgttcgag gccgggtgcc agatttgcaa agttcggtga
    19561 ccggaagata ttgcgaaatt cgccggtatc cgggtaaata cggggtcggc tcgccggttc
    19621 ggagcgaaga cgcccgtggg ggggtgtgcg gcccccggcg ctccagagag cgcactgccg
    19681 caaaagggcg ctccataccc accagctctc acccgaaccg cagtacttat ctccccgaag
    19741 atacatgggt gccgtgcccc tgccggcgtt gcggccggtc agggcggtga ggtgaagatt
    19801 gatggattca tatcggtgcg gactctgtgg ctacatctac aaccccaggg acggtgagcc
    19861 cgcccgtggt atcgggcccc gcacggactt tggcgatctg cctgacacct gggcatgctc
    19921 gcggtgcggc gcggataaaa acctgttccg gaaggtgtga ggggcaggtt cggggccggt
    19981 ccccccgcgg atatccggag gccttcgaga ctgcgaagcc gcgggggtgg nnnnnnnnnn
    20041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccgcacttat cgagccggcg gatcgtccct
    20161 catcggcatc cgtcggtctc tccggcggca cgcccgttac cgggggatga catggaccgc
    20221 agaggccttg aaggtgacgt gggccggtcc cccttcggcg agccccaggc gggcgatgct
    20281 ctgccgggtc aggacggcga cgaacggcac gccggcatcc acgacgaccc gtgagaagat
    20341 gccgttctgc cggacttcca cgaccgtccc ggagaaggcg tttacggcgc tcgattcgaa
    20401 ggcttcccgg gagacgatca cctcctcggg ccggatgccg gcatagacgt cgccctcgac
    20461 ggcggtgacg gtcctgaggg tgatggcgcc aaggtcgatc gccgcctcgc tgccccggac
    20521 ccgtgatatt ccggggaata tgttctccat gccggtgaag gcggcgacga actccgtggc
    20581 cggctgccgg aagacctcat ccggcgtgcc cgcctggacg atccttccat cctgcatcac
    20641 cgccacgcgg tcggcgaggg cgaatatatc ctcgaaatgg tgggtgatgt ggacgattgt
    20701 cgttcccatc gcacgatgga cgaccttgag ttctctcctg agccgatccc gggtgacggc
    20761 gtcgagcgcc gagagcggtt cgtcaaggag caggacgcgg ggccggagga cgagcgcccg
    20821 ggcgatcgcc gcccgctgct gctccccgcc ggagagcgtc aggggggtgc ggtcaaggag
    20881 atgaccgatc ccgagcaggt ttgcggtttc agaaacgcgc tccttgatcc ggtcgggttc
    20941 gatcttctgc tgccggaggc caaaccctat attctccccg acggtaaggt ggggaaagag
    21001 catgtagtcc tggtagacca tgccgatccc ccggtctttc gggtcggtgc gggtgatctc
    21061 ccggccgtcg aggctgatcg tccccgaagc gggcgcatag atccccgcga tcgtctcgag
    21121 gaggatggtc tttcccgcac cggtcggtcc gagtatgatg aggtattcgc cggcaccgac
    21181 ggtcaggtcc acgccgtcga gggagaattc gccgagggag agggagagag ccctgacgtc
    21241 gagcatcaga agaccccccg gccgtcggtg tagcgctcaa atatcgcaag cgacgtcacc
    21301 gatatgacga tcaggatgac cgacgcagcg atggcgagat cgaggtctcc cgtcgacata
    21361 ttcaggaaga gcgcgatggg cagggtctcg gtcttcatct gggtggcccc ggcaagcatc
    21421 aggaccgcgc cgaactcgcc gatggacttc gaccaggtga tgacgagacc ggcgatcaga
    21481 ccggcccggg ccagggggag ggtgacctgg caaaacgtcc cccactccgt gcacccgagc
    21541 gtcctggcga cgtgctcgta gcgagggttg atcgtctcga aggccgaccg ggtgaccctg
    21601 accatgtagg gaaggttgac gaagagctgg gcgatgatga tgccgagcgg cgtatagatg
    21661 atatcgaacc ccgcagccgt gagcatctgc ccgaactttg aagggccgta gaagatgagg
    21721 agcgctacgc ctgcaacgag gggcggaagg gcgagcggta tgttgaaaac ggcgtttacg
    21781 gccttctttc cgggaaacga gaatcgggcg agagagtagg cgaccgggat ggcggcgacg
    21841 acgcagaggg cggtcgagat cgccgacgtg atgagcgaga gccggacggc aaagaggatc
    21901 tcgggggagg tgatgctctc gatgagggcc gggagcggcg ggtagagtac aaggccgacg
    21961 atgacgacga ccatgtaggt gaccaggccg aacaggaccg cgaggaagag agccttaaac
    22021 ggggagggat gggggagagc actccggtcc cggatcacgg tcgaccagcc tggaacgctg
    22081 cgctctgccg cccgatcgct tcgacgtgcc ccggcgtcac ccaccccgat gttgcgtcgg
    22141 ggatgctgtc gaatgccggg atgtagggtt tgatgtcgag gataggcgtc tcgtccagga
    22201 gatcgatgcc ttcgacgacg atccgccttc catcgatccc gataagcctg gcgaccgata
    22261 tgccgagccg gttcgggcgg ttgaagtgcc gggtcgcgaa tatcccgtgc ggctccgccc
    22321 cgtcgatgag cggccgctcg gaaacctctt cgccggcggc gcggtggaac cggtagatga
    22381 ggatgatgtg ggagaaccct gcaagcgcga tgagcccgtc ttcgaacccc gggtagacct
    22441 ccaccgtccc gcgggcggtg gagaatacgc tctggatggg ggttttggcc gggtccgaga
    22501 acggcgtgcg ggcaatcccg atggccttgc aggggtaatc cccgttctct ttcatggctc
    22561 gatccctgcg tacttcgggt ccggataggt cgggaacccg tactttgcaa agaaggcctt
    22621 cccctcatcg gaggtgacga acgcgacgaa cgcctgggca gcgtccggtt gcgtcgtgaa
    22681 ggtggtcggg cctatcggga cgatgagggt gaggctgtct tcctgcacca gatcgatggt
    22741 atccatcgtc tcggggttga cgctgtcgag cgtgaggagg gcggcatcgg cggtgcccat
    22801 ctccatcgcg atcaccactt cattgatcgt cggtgccctg aggacgacgt tttcctcgac
    22861 ggcatcgcgg ataccatgtt tttcgaagag tatatcgccg gctttgccga tcgcggtcga
    22921 ttccacgcca ccgagagcaa gtttcagccc cggccgggcg agatcatcga cagaagtgat
    22981 attcttcggg ttccccttct ggaaggcgat gatcgggacg tggtaggcga cgtacccggg
    23041 ctctccgacc agccccttat cccgggcgat acggtagtcg ggtgtcccgc cggagacgaa
    23101 gacgtcgccc ttccgggaga gctccatctg gctgacgagt gcccccgcac cggcgaagtt
    23161 atattcgacg ttgatgccgg tcttctcggt gaacgccttg ccgatctcgt tcatcggttt
    23221 cttgagaccc gcgcctgagt agacgaagag cgatacatcg ctcgcaccgg gggtagcgtt
    23281 ggtgtcggtg gtggtcggtg attggacggt gcagcctgcc gtccagagga gggcggcaag
    23341 gatgatgagt gcagccggca cgatgatctt ctttctcatg gtactgaacg gtacccacct
    23401 gccggtatta aagaactatt atttactttg aggttatgtt aaactgtttt atctgacata
    23461 ctccctgttg aaggttgcta tcctccccta cccggttgcc gtacccccgg tatctgcagc
    23521 ggtcgcgatg gggcggtaca gggtgtcctt tgcggtcgac ccggggcccc gccggggccg
    23581 tcagcagaac cggccgtccg atcgcatggt gcgggccgcc cccgatgtcg cggtcagcca
    23641 cccggcgcgc tcttcgggaa acgcgtcgaa ggccgggacg tagggtttga tatcgaggac
    23701 cggggtgccg tccagtatat ccacgtcctc gacggtgagc gtcgcgccgt cgacggcgat
    23761 caacttgagc atcgatatac cgactgcgtt cgggcggcgg ggtgctctgg ttgcaaagac
    23821 gccgcgaggc gtcttgtcga ggaacggaac cacctcaagc gcgtagccct ccgaccggtg
    23881 gaagtggtag aggaggatga tccgcgagaa cccttcgagg tctctcagtc cggcggcata
    23941 ggtcgggtca agctcgatgg taccccgtat gccccgggcg ccgacgggct ggatgggcat
    24001 atcgcgtggg tcgtggtggg gggaacggac ggtaccgata ggggtgaatg cgatgccggt
    24061 catggatatt ctcctgaggg cgttatgcgg cggaagtgaa gagggtttgt gtccgggcag
    24121 gatcctgcct tcaggaaccc gggagccgga ttcagcggcg ccccgccttc gcggcggtga
    24181 tccgggcccg gaggtcccgt gccgcggcgg tgacgtcatc ctctcccacg accgccgata
    24241 tcaccgcgac gccatccgcc cccgcggcga tgacgccggc gacgtttgcc gtggtgatcc
    24301 cgcctatcgc gacgaggggg agggaaatcg tggcccgaat ctccgcgagc atcgtaagcc
    24361 cgtatcccgg acccgcgtcg tccttcgatg cggtcgcgaa cgtcgggctg agcgccacgt
    24421 agtcggcccc ctccgccgcg gcccggaccg ccgtcgcgac ggagctcacc gaggcaccga
    24481 tgacgaaccc cggcggggcg atccgccggg cctccccgac ggggaggtcg ttctcgccga
    24541 ggtggacccc gtcggcgccg gccgcgaggg ctacgtccag gcggtcgttc acgatgaaga
    24601 gcgcgccggc atcagcggtg atcccgcgga tggcgacggc tgcccggaag aggtcgcgac
    24661 cggagagcct cttatcccgg agctggataa cgtccgctcc cccggcaacg gcccggcggg
    24721 caagctccgc gtgggagcgc ccccggccga tctcctcatc ggtgatcacg tagaggtcgt
    24781 atcccattta tcgctcctcg atccgcgcgt ggcaggcgag gtcctcgggg gagagcccgg
    24841 caagttcgtc aaagagcgcc gtccggaacg agtagggccc gcgggcagag accgccgccc
    24901 gctccccggc cctcccgaag gctgcaagcg ccgcggctgc cgcgaccgcg taatcatccg
    24961 cgacagcgac gaacgccgcg gtgaccgatg cggccatgca ccccgtcccg gagaggcggt
    25021 ccatcgccgg gacaccgttc ctgacgagga agacccgctc tccatcggtg acgacgtcga
    25081 cttccccggt catcgatacg acggtcccgg tcaggcgggc acactcccgg gccgtctcga
    25141 tggggtcgcc ggcaactccg ccggagtcca ccccccggac gctcccgccg gtcccggcaa
    25201 ggacaccgat ctccccggcg ttccccttca ggacggcgat ctcgagggca ttaaggaggc
    25261 gctgcaccgt cccggtccgg aacgccgtcg cccccgcacc gaccgggtcg aggatgaccg
    25321 ggatgccgag gtcgtttgcc cgccggccgg cggcgagcat cgcctcgacc tgcgccgccg
    25381 agagcgtccc tatgttcagg acgagggcgc cggcggcggt gaccatatcg gccacctcct
    25441 ctggcgcctc ggccatcacc ggggcggccc cggtgcagat cgttatgttt gcgcagtcgt
    25501 tgaccgtgac gctgttcgtg atgtggtgga tgagcggtcg ctgttcccgc acggcggcaa
    25561 ggagggcggc ggggatcgtg ccgtccatga agcgtgccga tgaaccgtga ttcgtgctac
    25621 ccggcataga taactatctg tttgagcggt tgcataaaac cggtcgccgg atctccgcgg
    25681 gcctcacctg cccttgcagt ccctgcaccg ggccgcgcgg ccggccggag aatactctct
    25741 cgcatgtatg ccgggatagg aattgctaaa gcgatctgca ccccacccct ccgcgaagag
    25801 aggtgaggac gacgctcagt atcaacgaga cggcgatgcc cgtcgtgaga gagatgatcg
    25861 gctaccggga cgaactcggg atcgcggtcg cggaacagga gaacggcgcg acgtgcatcg
    25921 actgcggcgt gcacgtgccg gggggctacc gggcgggggc cctcttcgtc gagacctgcc
    25981 ttgcggggct tgcgacggcg gcgataacca tgggccgcgt ggggagcgtg cccatcccgt
    26041 ttctgcacat caccgtgagc caccccgcgc ttgcatgcct cggttcgcag aaggcggggt
    26101 gggtgctcaa ggccggaggg tattctgcga tggcctcggg tccggcgcgg gcgctcgcgg
    26161 caaagccgaa agatacctac aagacgatcg gctaccgcga tacatcggtg ttcggcatcc
    26221 tcgcgctcga ggccgatacc ctcccggacg aggaggtcac ggggtttatc gcggagaagt
    26281 gcggcatcga ccccggagac ctctatgtcc tggtcgcccc gaccgcgagc ctggtcggtt
    26341 cgatccagat atccgggcgg gtcgtcacgg cgaccctcca caaactcgag gcgaagggtt
    26401 acgacgtctg ccggatccgc cacgcggcag ggagatcccc gatcgcgccc gtgaagagga
    26461 ccggcatcgc ggcgatgggg gcgaccaacg actgcaacat ctactgcggt tcggtctccc
    26521 tcatcgtcga gggttacgac ccggtctttg cgagcctccc ctcgcggacc tcccccgact
    26581 acgggaggcc gttctcccgc gtcctcgaag atgccggcta cgacttcctc aaggtggact
    26641 cgctcctcgc gttctcgccg gcggaggtga cgatcaacga cggcacttcc ggcgaggtct
    26701 accacttcgg cagcctaaac cccgatgtgc tgcttgagtc gtttgagatc cggtgagaca
    26761 aaaaggggat gggatccccc ggctcactcc ggggtcgtct tctcgcggta gcgcaggatg
    26821 gccttctcga cgtaggacgt catctgcacg gcgccgacga cgcaggcctg tgcgcacttc
    26881 ccgcacccga tgcaggtctc cgggttcgtg atccaggcct tgtcgatctt cctcccgagg
    26941 tcgacgagct cgatcgagcc ggtcgggcag gcctcgacac agtcgccgca cccctcgcac
    27001 gagaagggga tgatccaggg gaatttgtcg ggcaccttct ttctgtcttc gctcatcgtg
    27061 cctcagttcc tcttccgcgg cacatccttc cagcgctgcc gctcccacgc atcgagttcc
    27121 tccggcgtca tgccgtcgag ccggagcccc gcctcgtagc cgggcttcgt gcagtagagc
    27181 tcggtcaggc gcacgaggag caccgctccg gcgtcgatcc ctttctcttc ctttgcccgc
    27241 gccctgaccg catcgaagat ctctcccgac cggaggagct ccccggtccc cttgaactgg
    27301 tatgcggcgc ggatccctcc ctgccgcgat acgccgaacg cgacccgcgg gtttcgcgag
    27361 agcacggggg cgatcacccc tgcctcgtcc tccgcaagcg cgaatgccac ctcaccatcg
    27421 gctgtcgccc gggcatagcg tcccagcacc acgaacggca cgccctccgg cgtcgcgacg
    27481 cagagatggc agcccatcgc ctctacccat gccttcatcc tctctgtaag gttcaccgtc
    27541 atcctgatca cctccatact acaggatctt ctctcccacg tgcccgggct cgatggagta
    27601 gacctcctcg acgtgcacga ccacgacccc tcgctggacg gccttcggga agacgatatc
    27661 gggcggcacc tcgtccggcg gttcggcctt gttccagttc ccccattcgt tcaggatatc
    27721 cgtcgcgttc gcgccgtacc agtcgaaatc gcgcgggaac ccgtactcca tatccacggc
    27781 tttgcccttg aagacccacc agcgcccctc gtcgagcggg tggcagatcg tgaccgcgac
    27841 gttcgggttc tccttgatgt tcgccttcgt cttcaagagg aacatatcgg cgatcaggat
    27901 cttgtcgtcc atgtgcgtgg caacgaaccg catcgcggcg atgttggggt tgccgtccct
    27961 gctcgacgtg cagagataga tcaggctccc tcctttgccg ggaacccgca gggcttcttt
    28021 catctccttt gtaaacttca ccatgccttt cacctcatag ttacttcacc ggatacccga
    28081 acgcggcggt cccggcgggc ccgagatcct cgcccagcgc acgttttacg agctgggtga
    28141 tatagatcgg ctcgagtttc atcttccggc aggtcgcgcg catcacggag atgcaggccg
    28201 gacagatgaa gaggagctgt tcagccccgg cgtcggcagc gtcctggacg ttcttgcgct
    28261 ggatatcgac cgcccgttcg tgctgggtgt gaaagatacc gcacccgcaa cagagccggt
    28321 caactccggt atacacccgc ttctcctcaa cggacgtaac gccgatcatg gcgaatatgt
    28381 cggcaagcca gtccgaccag atctcgcggt ccccgccctt cggggcgtag cgcgtcgtgc
    28441 aaccaccctg gaccgcgatc cggatatcga gcgggttcac gatccggtcc gggtgatcgc
    28501 gcagccagtt cctgatgtac tcgaggatat gcaccggccg aaacggcacc cgtaccccct
    28561 gctgcatcgc aagggtcgtc gccacgttgt agcaggcatc gtgggtaaag acgatctcgt
    28621 cgaccccgaa ctcttttgcc gttctcgcga ggttctcgat gaactgcgga aggaacttca
    28681 ccggccggga cgcccggcca agatgggtct cggtgaaccc acaggcatat ggcccgccac
    28741 ctatgatggt tgcgtccgaa aagacctgtc ccgtgaggaa ctccgcctgg ggcaccacct
    28801 cgtagatacc gccggcagag atcaacgggc cgcccggctt tcctctccgg atgaccgtcg
    28861 ccggtttcgt cagccagtcg ctcgacggtt ttgcatccgc cgggatgccg agaacgcccg
    28921 tctcctcctg ccgctctgcg atgaggtccc aggggtttgc gccccgcggg cagaagtcgt
    28981 tgcaggctgc gcaggtgatg cagtctttca ggatcgggtg gggttctcct gcaatcaggg
    29041 ctcttacggc cctctttgct tcatcctcgt cgtatgagac atacggacag cggacgaggc
    29101 attctccttt gcagcgtgaa aagttgcatt tcgagagatc aaatgccatc actcagccct
    29161 ctatttaccc tgtgtccgta tccttatcta acgtaggttc ccggcaatgt gagatcccgg
    29221 gacgatttgc gcgccccttc cggcagggaa cgtttcggcg agcggccgcg gagggtatcg
    29281 gcgtgcgcat atcgctattc tttttannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29401 nnnnnnccgg atgaggccga ccgcctgcgg ataaccggga aggggtcgcc cgtgagaaag
    29461 aggtgattgg tgtgaagaag tttcgtatag agctgacggg gttccttgag atggataccg
    29521 accaggagcg cgatgcacga gtgctggcgg agcggatcct cctcgagatc cgctcggtcg
    29581 ttgccgggca tggcggggtg caggactatg acatcgatat aggttcggtt acggaaaaac
    29641 cgctcacgaa gcgctacagg taaccaaccg caggactcac tcgagatcgt gccgccggag
    29701 gaactcgcga accttccgtg actccaccca tccccggtcc agctgccgga tgtagtcgtt
    29761 gatccgctcg acctgctcgc ggatgactgc tgccgtctcc gtatcgtcga tcagctccgc
    29821 cctccccagg gtcacctgca ggggctggcg gatgtggtcg ccgaggacgg cgaactgctc
    29881 gatattccgc tcgatctggg agaacgcctg ccggcgaagc tgttcgttct gtttccgttc
    29941 ggtgatatct cttcccactg cctggacgcc gaccaccttc ccgtgctcga ggatcgccga
    30001 ctcgttcagc tcgacgaacg ccacgaaccc atctttccgg tggaactcga cctcgagccc
    30061 ctcgaccggt tcgccccggg ccagcttctt ctctgcctcc tcccacgcgg ggagcgatac
    30121 ggggctgagg taatcgcggc atttgcgacc gatcaactcc tcgggcgtgt aaccgagcat
    30181 gcgggtcacc gccggggata tgtaggcgat cccgctgtcg tggtagcagg tgtagatcat
    30241 atcgaagctc cgctgcgcta tcgcgcggaa cttctcttcg cttgcgagga gcgcctcctc
    30301 ggttgcccgc cgctcgatcg cctgcgcgag aatgtttgcc gccgcctgta tgaagttgat
    30361 gtcatcctgc gtgaacctgc ggtgcgtccg ggtgtgggcg ccgagcaccc catagggtgc
    30421 cctttttccc tggatgatga cgctgatccc gctcacgatc ccctcgtccc ggaggagggc
    30481 cgggccggta aaacgtgtct cggtcctgag atcctcgacg ataaccggtt catgggagag
    30541 gagcgtgtag cctgcctgtg agtcggttcc gccctccacc ttctccttgc cgacccgggt
    30601 ctcgttaaac cccaccgccg cctcaagcag gaatgcgtcc tcgtcgggga gataccggag
    30661 caccttggca tacttgacgc cgaggtgccc ggccaccgcc cggaccaggg cgttcatcaa
    30721 ctccgatagg tcggtccccg caagagcgag gcggccgagg tctgcgatga ccgcctgttg
    30781 cgccgcccgg acagcggtga tctgttcggc ctctcttctt gcggtgatgt cgagattgat
    30841 gccggcccag aggatgatcc ggccatcttc atcccggatg gggccaccgc gcgagaggat
    30901 ggtgcgatac tccccgcctg gacccggaac cctgagttca cggtcccacc ggcacccggc
    30961 atcaaggcag cgcccccagt cggcgagcac cccggccgga tccccaagcg gtgtgcggcc
    31021 cagccacccg gactgccggc actcctcaag cgtcgtcccg ataaggtcga ggaacgaagg
    31081 ggagatgtag agcacggtgc cgtccgggtc gcatatccat atgccgtagg ggacgagctc
    31141 accgatcccg gcgaatatcg tctccgctcc gacctctgcc tccacttccg aagcgttcca
    31201 tgccgcctca ctatacccgg acgtgatatc ccggagccgg acgagggcca tcccggtgta
    31261 gggctcctcc gtcatcatac ggcacgagaa tgagaaccag cgcacctcgc cgtcgggggt
    31321 ctctatcctg caggcggcgt gcgagacctc ctgccggtac cggagagctt cgatcacccg
    31381 gtcggcgccc tctccctcca ggaggaatcg cgcaaggtgg ctggttgtaa actcttccgt
    31441 ctccatgctg cggaccgtct cgtcgtcgat gccgaagatc tcctggaacg atcggttcaa
    31501 ccgcgcaacg gtgttatccc gatccaggat aatgacagca tcgtccagtt tgtcaaagca
    31561 gcggtacacg ggtgatcccc atcggtcgat accaatgccg gtactgatgt accgggttta
    31621 tgaatattac tttctggagc gctcgcgatc tgctgtggta ccaggatcac aggcagtggt
    31681 tcttgaggat ggaaaccgga tgagagggat cgcgcacccc cggtcgttgt agagagacgc
    31741 cgagggggag atttgaactc ccgaggtgcc aggcaccagt ggctttcgag gccaccgcct
    31801 tcccggacta gactacctcg gcataaaacc agcctacagt atttaggttc tccggctata
    31861 atagtatcga ggtatgctct gtcgatttac cctcatcccg gatgggcgcg tgctgatcta
    31921 ccggggccgt ccgggcgaga cctttgaaga cgccctcctc tcgtttgagc tcaaccccga
    31981 tacggcgctc atattctccg gcggaaggag cctgccgcag gataaaaaga tcgaggaaga
    32041 ggaggtcgag atcttcctcg cttcctcgcg cggatgatta ctgcgcgccc ggcatccccg
    32101 gctttccagc ctgggtgacc atcatgtcgt cgacgcggac gaggagcatg gccgtctcgg
    32161 tcgcgctctt gatggcctgg gtcttcacgc gctcgggttc gatgacgccg gcctcccgca
    32221 tatcgacgat cgcgccggtg tagacgttga gcccggcgta cttcgcgccg tctgcgtggg
    32281 ccttccggag agcaacggcc ttgtcgatcg tgtcaaaccc ggagttctcc gcgagcgtcc
    32341 tcgggatgac ctcgaacgcg ttcgcaaagg cttcgatggc aagctgggcc cggcccccga
    32401 cggttgccgc gtagtcgcgg atgcggagct gaagctcggt ctccaccgac ccgccgccga
    32461 cgacgaacct gccgtcctcg atggcgtcct ggacgacccg ggctgcgtcg tagaccgccc
    32521 gctcgagctc gtcgagcagg agctgggagg tgccccggag gaggatcgtg acggctttcg
    32581 ggttgtcgca cccggatatg accgtcagtt cggtgtccgg gagctcttcg gccgcttcgg
    32641 cgtggccgag cgtcgttgcg ttgagttcct cgggcttgtt gacgatggtg ccgcagagtg
    32701 cccgtgctgc gaacttcatg tcctcttcct ttacgtcctc gatggcatat atgccgctct
    32761 tcgcgagata gtactggacg gcatcggcga tccccttctg gcagaggacg acgtttgcac
    32821 ccgaggcggc gatctgatcg gcgatcttct tgagggactc gcgttcctgc tcggtgaacg
    32881 ccttcatctg gtctgaagag gtgatcttga tctttgattt tacctgggtc ttcgtgacct
    32941 cgagcggctg ggcgagcagg gctaccctgg cgtcggtgac cgcgtccggc atctgttcaa
    33001 agacgcgctt cttgtcgatg acgacgcccc ggatgagctc cgcgtcgtgc atcgtgtcgc
    33061 cgacctgcgt cttgatcttg atgtcgtcct cgtccacaat gtaggcgccg gcgcccgccc
    33121 cggtcgcgac ctgccgcacc gcatccacga cgatgttgga taccttctcc ttgaccgcct
    33181 cgatggactt gccggtcatc gcggtgtcgg cgatctttat gaggttctca cggtcttcaa
    33241 acccgatcgt tatggccagg tcatcaagaa ccgcgagggc cttctccatg ccgagctggt
    33301 aaccgttcgc gatgaccgtg gggtgaacgt tctgggcgag cagtctctcc gcctcttcca
    33361 tcagcgaccc gaccagaacc gtcgcggtcg tggtgccgtc gccgacctcg tcgtcctggg
    33421 tctcggcgac ctcgacgacc agcttggcac cggggtgctg cacggacatc tcgtgcagga
    33481 tggttgcccc gtcgttcgtg atcaccacat caccgctgga gctgaccagc atcttgtcca
    33541 ttccccgggg accgagcgtt gtccgaacgg cggccgcaat tgcttttgca gccatgatat
    33601 tcgagcgctg cgcctcgaat ccgcgcgtgc gctcaacatt
//
LOCUS       DAOSII010000004        31830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_1605_length_31830_cov_49.901747, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000004 DAOSII010000000
VERSION     DAOSII010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 31830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31830
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1605_length_31830_cov_49.901747"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PLV90_00680"
     CDS             <1..230
                     /locus_tag="PLV90_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95655.1"
                     /translation="EGSARTRAYRTQGIGISMLHNIHSWYREQILPEKDEVASRRVGR
                     SREGIKNLILVPVEEIRVGGCGTFSLFANRL"
     gene            501..890
                     /locus_tag="PLV90_00685"
     CDS             501..890
                     /locus_tag="PLV90_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95656.1"
                     /translation="MQLKQSVLLALGILLVLAGVVLQYTGIVVESTATVALISAGVAM
                     VTIAVVMGLRRQDEVISDERTKRIGAYGTTYSWVLTLVLILAFLWLDFLGLVSFNLQG
                     TLLVLLFTMLISARIFQWYLFRQGDVA"
     gene            940..1146
                     /locus_tag="PLV90_00690"
     CDS             940..1146
                     /locus_tag="PLV90_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOF95657.1"
                     /translation="MKTRIRELRAKTGLTQEELAREVGVRRETIVFLEQGKYNPSLKL
                     AYKVARVLGASIEEVFMFEEEDLA"
     gene            complement(1567..2112)
                     /locus_tag="PLV90_00695"
     CDS             complement(1567..2112)
                     /locus_tag="PLV90_00695"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HOF95658.1"
                     /translation="MIGNLLITGRPGSGKTTLMRRLAERLRDCSPVGFYTVEVREGGV
                     RVGFDLVSLAGDRRPFARTGYPGRCRVGRYGVDIEGFEDFLSSIPFFTPGTRLAFIDE
                     IGKMECYSGVFRGIVREALDAPAPCVATIAQRGMPGLDRIRGRADVRVVEVTPSNRDD
                     LLPELEAEARRLVGDAGQGWK"
     gene            complement(2162..3214)
                     /locus_tag="PLV90_00700"
     CDS             complement(2162..3214)
                     /locus_tag="PLV90_00700"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOF95659.1"
                     /translation="MPGGSNPPGRMGYPFDRAGRGWRAGGRGLFRESIAPGCDQARHI
                     RDDLIAEYRGLTVEDAFSGREVACPTGSCYVLESRSPMTEGLDSRDPERAAAAILADL
                     TLVPGIGGATRQRLQRQGYRTIADLVRHPLYCRDAARILDGVLGRDTCGLMEWIGRRH
                     RQSDPLLLEVSRFHAPETFVFLDIETLGLSSRPIVLIGLARVEGDAIAIRQYLLRSLN
                     EEAAALATALPDLEAEGAALVTFNGRAFDLPFIRERLVRYGIPADLGAPHFDALLFAR
                     RRWKGSIPACRLNVLETEVLGVERQDDLPGWMVPEFYDIYRRTGNPGPLIPVVEHNRQ
                     DLVSLARLFALLRGDE"
     gene            complement(3258..3737)
                     /locus_tag="PLV90_00705"
     CDS             complement(3258..3737)
                     /locus_tag="PLV90_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95660.1"
                     /translation="MDVRWKLAAREISALPLAYRRAIRDGADDYLYNEVERAAWQEQG
                     REVGVIARAFGMPTGNARGVAEAFAAATRVFFGPEYRYEIQESGSDEALLTVRGCAMV
                     ARADERGEDPLTVCNACHAYANAAIPALNPDYAPKYRSSICAGDRACSIAVRPAGRE"
     gene            3882..4046
                     /locus_tag="PLV90_00710"
     CDS             3882..4046
                     /locus_tag="PLV90_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95661.1"
                     /translation="MKVRAVPIDIGGARTSGTKGIPAGTDRRRERAVLRSVPVPDLVV
                     GLLADPLDHP"
     gene            4051..5154
                     /locus_tag="PLV90_00715"
     CDS             4051..5154
                     /locus_tag="PLV90_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95662.1"
                     /translation="MERKFLATLILVAMALGAGCADILPGPPVALPAIVPVEGASLDP
                     LPAHTFPFEGGEETIRIDPDPAVYAGAKGADRRLHLARDLSREEWIPLYYRAFESEPH
                     LEPFYADLLAALRDIRDREGLDDDRYLELIAVFVQSIPYRTDASIVEPKFPIETYVDG
                     EGDCDDRSLLLAGLLAREGYGVALFYFEDEAHMAVGVQSTGCHYRNTSLAYVETTNVS
                     YVGIPPLALSSGVVLASDPLVIPVGDGPRLYTGCDQIETIERALSSSLARAGALAPEL
                     AARTRDIEAERESLEAFGTRLKALSRDGEFLEYNRLVPEYNRKTREYNDAVQAYNALL
                     AESRAAVDLHNHLVTHACDRPGSYLRARAYLAG"
     gene            5611..6342
                     /locus_tag="PLV90_00720"
     CDS             5611..6342
                     /locus_tag="PLV90_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF95663.1"
                     /translation="MLDISDLHVNVDDTEVLHDINLHIGQGETHVLMGPNGSGKSTLL
                     KAVMGFGGYTITAGSIVFRGTDITHMPIHERAHLGIGMMFQHPPAISGLKLGKLLTAT
                     SHMGEGAIEALAQSVNMERFLTRDINVGFSGGEIKRSEVLQLKVQQPDFIMLDEPESG
                     VDLENMNLMGKEIAGLLEKDVHIVNRRRSGLIITHTGYILDYLEADQGHVMISGRIRC
                     HGNPREILRVVKEKGYGECLRCKQI"
     gene            6327..7439
                     /locus_tag="PLV90_00725"
     CDS             6327..7439
                     /locus_tag="PLV90_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOF95664.1"
                     /translation="MQTDITDMERLSQEDRERLALTGLEVGMQNRCGSFFQIDQKVIQ
                     TTCGAEGIEMLPIRAALEKYDWVKDYYWNAVSKDKDKYTQFVAKQENPQGLVVIAHEG
                     AKVAMPLQACLFLRDEPVQHVHNIFIAREGSEMHIISGCASSWQKEHGAHIGVTEIYV
                     GKGAKVTSTMIHNWNPGISVFPRSATIIEEGGVFLSNYVCMQPVNRVDMYPTARLVGK
                     NAVARFSSIVVATPGSILDLGSRAILDAEQTSAELVTRAITTGGKVISRGHILGEKDG
                     TRGHIECKGLILKDGIIHAIPEIEGTLTGTELSHEAAVGKIARHEIEYLMARGLSEEE
                     ATATIIRGFLDVKISGLPEVLQKQIDAAIDKAESGF"
     gene            7458..8123
                     /locus_tag="PLV90_00730"
     CDS             7458..8123
                     /locus_tag="PLV90_00730"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOF95665.1"
                     /translation="MTKTDPYEREREAMVEHQIRDRGIRDERVLAAMREVPRHLFVPK
                     GYERAAYEDRPLPIGEGQTISQPYIVAVMTEQLGLAPQDRVLEIGSGSGYQAALLAGL
                     AGTVISIERLEGVAEQARENLARAGVTGVRIIVGDGTEGYPPEAPYDAIIVTAASPGV
                     PEPLIEQLAEGGRLIAPIGPRDCQDLVKLVKREGKVETIPLGGVCFVPLIGQFGWRGE
                     GFR"
     gene            8120..8728
                     /locus_tag="PLV90_00735"
     CDS             8120..8728
                     /locus_tag="PLV90_00735"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOF95666.1"
                     /translation="MIIYDITRDLSADAILYPGDIRPRFREIDNGQYRVTEMTIGTHS
                     GTHIDAPSHYIEGGLTVDRIALGVLAGSARVIDCSDAETLIGPENLADRLAGARTLLL
                     KTWFSGREEFDPGYPALSPEAADLVVEAGITCIGTDAPSIEAFCGDGSVHRRLLGAGT
                     VVLELLDLAAVPEGEYHMVALPMRLAGADGSPVRAILCNGKE"
     gene            8730..9335
                     /locus_tag="PLV90_00740"
     CDS             8730..9335
                     /locus_tag="PLV90_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95667.1"
                     /translation="MSFLNDAIRRLEHILADSGCENGIVDLSVNYNADVCQYPKGVCM
                     EGHFGGRTGQFVTSEPIRARTRISFMFGAPLTDQKQRGAASAIINAVSAFLCLARKLR
                     PCTPDCYAPCLADLSREIGRKRIYPVGPMPVIERAFKDQIVDSPEEADILLVAGEGMT
                     TDAGIACIDGCRGNKRIIFLGPSTAGVSGLLNLEHWCPYGR"
     gene            9373..10641
                     /locus_tag="PLV90_00745"
     CDS             9373..10641
                     /locus_tag="PLV90_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase/phosphoglucosamine mutase"
                     /protein_id="HOF95668.1"
                     /translation="MLFGSSGIRQEFGPGLVDLALRIGAAAADGASPLVVGRDTRTTS
                     ELLEHCVVAGMLSAGAAVYTCGIAPTPTVAYATRRADAGCMITASHNPEPYNGVKLLN
                     PDGSSFTRRQQAAIEEALEGTHRVGWEEQGHTSPIDALAMHRDAILGEISLSRPVTVV
                     LDCGNGAGSVITPDLLSDAGATVVGLNCNISGRFARPSEPLEANLPYVGEIVRKRGAG
                     CAVIHDGDADRMMAFDERGRYIDGDHLLMLFAAYLDHKRVVTTVDASMAIEEVAEVRR
                     TPVGDTFVSEELLTWGDFGGEASGSWIFPGHSYCPDGVYAAALLCEIASEWSIAEELD
                     RMPRYTLLRESCRVASPGEIMAAIGADEPTHGVRFSDDDGWYLIRASGTEPKVRITAE
                     GKTPEKAKEMLGAGHAALARAKRILGGREE"
     gene            10647..11846
                     /locus_tag="PLV90_00750"
     CDS             10647..11846
                     /locus_tag="PLV90_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOF95669.1"
                     /translation="MQCVVLAAGEGKRMRPLTARRPKVMLPIANRPIMEHLVVAARDA
                     GITDFVFVVGYFEREIRNHFGDGSSLGVKITYVTQRHQLGTADALRATAGMVNGRFLL
                     LNGDMVLKRSDIERFCRMSVPCMGIHETDHPEDYGVVTVEGGRITGLEEKSERPKSNL
                     INAGAYLFDPDIFDLLAGVKISGRGEFELTDALESYIREGTLAAHPLDYWLDVGQPWD
                     LLDANEALLSSLCCEWRGTIEDGCTVPDTVSIGEGTIIRAGTYIEGHCVIGENCVIGP
                     HAYIRGSTAIGDGCHVGHATEIKNSIVMARTNIPHFNYVGDSIVGSGCNFGAGTKVAN
                     LRHDNAAVKVCGKSTGRRKFGAIIGDNVLFGINCSVNVGSLIGSNTRVAPHSLVEGCI
                     EEGSVIR"
     gene            11851..13002
                     /locus_tag="PLV90_00755"
     CDS             11851..13002
                     /locus_tag="PLV90_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOF95670.1"
                     /translation="MQAVILAAGEGSRLRPLTRSKPKSMLPVANRPIIEYVIDALLEN
                     GIRDIIVVVGYRKEDVIRHLNRLDAPIQVVVQERQLGTADALRAAESEITGDFLVLPG
                     DNYVNAESIARIRKERNAMLVAEHPSPSNFGVVVIRNGLVREIIEKPEDAPTFTVSTG
                     IYSFSPDVFSCLRTNEIPDALAAMIAAGHRIRAIPADDWQDAIYPWDLLKLNNRMLRE
                     ISPEVGGTVDASVIRRGTVRIGTGTTVGPNTVIYGPVAIGDNCNIGSNCVIMPNASIG
                     DRVEIEPFTYVADSLIMSDARIGSHSRIVSAVFGQGCILADHTTTYPSASFIEVGERV
                     QKEEFGAVLGDGVRAAPFTTFKNCIAGNNVTIERGKTVVGLIEDGTRVM"
     gene            13003..14742
                     /gene="glmS"
                     /locus_tag="PLV90_00760"
     CDS             13003..14742
                     /gene="glmS"
                     /locus_tag="PLV90_00760"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOF95671.1"
                     /translation="MCGIVGYIGRRDATGVLIQGLKRLEYRGYDSFGLATVGSAIEVY
                     KKVGRISDGEAGATGLAGYAGIGHTRWATHGEPNDINAHPHTDCTGRIALVHNGVIEN
                     YSELKRQLEGRGHVFRSETDTEVIAHLIEEQYDGDLLAAVSAILPRLEGSYAVLVIAA
                     DTQKIVAARNASPLVLGVGDAEVFAASDMTPLLEYTERVVYLEDGDVADITPDRYTIY
                     YDGREVERPIELISWCVEDTRKGGFAHYMLKEIFEQPQSFYETIRAGIDDHVRQMVME
                     ADEITLVACGTSYHTALVFKYLAEPLCNLPIRVEMGSEFKYFTPPLHGLVIAVSQSGE
                     TADTIAALKMAKARNCPTLAITNVQGSTVTRIADETLLMRAGPEIGVAATKSYTAQLA
                     ALMQIVNARCDDAFDDVLSHAHLAISEVLIREIREAVALCAKAEHVFFVGRGPFYPVS
                     MEGALKMKEISYVHAEGYAAGELKHGPFALLTPETPVVAICTPGPTYSVMASNIKEMK
                     ARRAPVIALGVDGDKELAEIVDIFIPIPNTHLLVQILTISVTLQLLAYHTACALQRDV
                     DKPRNLAKSVTVE"
     gene            14739..15332
                     /locus_tag="PLV90_00765"
     CDS             14739..15332
                     /locus_tag="PLV90_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HOF95672.1"
                     /translation="MIEYGRNALGEGATIFEPVTIGFPSRDRMGEEDYPGAAIGRNAI
                     LRSGTIIYCDVVIGDAFQTGHNVLIREKTTIGDRVAIGTAAVVEGDCTIGDDVRLQSL
                     VYIPTGARIGNRVFIGPNAVLTNDRYPPGPREGLRGPVIGDDAVIGANATVLPGVVVG
                     EGAFVAAGAVVTKDVPPTMLAVGAPARFRPLPSGAKR"
     gene            15486..16628
                     /locus_tag="PLV90_00770"
     CDS             15486..16628
                     /locus_tag="PLV90_00770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOF95673.1"
                     /translation="MKILLVSTQDYIHHPIPSRHHNIFEELATRHEVHVPHFHVSRGK
                     ERKTLLHVHEATRFAVESPFLHYTLNAPCHYRVIRDIIRDHEIDVVVGAHVLAGTAMV
                     RAAEKSGVPVIFDLKDWFPDSAAAYYKNPAMQWLLREGVLAITRYNLDHSDVITTVSP
                     GLVEKLRTYGYEAELITNGVNTELFRPMDGRKMRSTLGIAPDAFVLGFAGAVERWYAL
                     DEVIRALPDILARHSNAELLIVGGSLFTGYLDELRTLAADLGIARRVHFTGAVDYCDL
                     PGYIAPMDLCLIPLSPPQWVDIALPNKYFEYSACGRPILSTPIPDMLRMGGGSIAVYR
                     DKGEFIEKVDELAGNPGRCPLGIEEHSWKKKAEAFEKVFARVMKKA"
     gene            16714..19359
                     /locus_tag="PLV90_00775"
     CDS             16714..19359
                     /locus_tag="PLV90_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006487 - protein N-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharyl transferase, archaeosortase A
                     system-associated"
                     /protein_id="HOF95674.1"
                     /translation="MAHVDLDKYRPYIIIGVIVLLTLLPLWVRGFIPAADMVTPDGVN
                     LLGNDPWYNVRQVEQTVANFPGYAWFDAMTLYPSGDVVYWGPLFIQIISALCVLVGAT
                     TRPEIMVVASWVPPLMGAAMVPVTYLLAKRLTNWKTGLIAAGLIAVVSGSFAHRSLFA
                     FVDHHIAETLFSTIFVLAYVVALLASRDISLSRRSSEVLKMPVITAAFAGVAYLIGFF
                     NMPTMILLALIVAIFTLVQFVLDFVQDRPSDYLVLVNAVTFGVVIIGTAAFGFPHTGL
                     DLSRYTVGHVVACAGLIGGTLVLYGLSVYLRGRPKYYFPAALAGLGVVAVAVLSVALP
                     DIYNLLISSLFAFFGEAPVTTTVQEARAWSFDAAWATFHWGLVLAAGGAATLIWWSRK
                     KVNPAHVFILVWTAIILASTAAHVRYEYYLAANIALLAAIFAGAILDVTYKDVTRLFG
                     TKSSTAPPDAEVARKEEKPARKGKKGARAPDARKTKVSSKDQPDYIKVGAFAAVVVVT
                     LVFAGTSLQTNFAYAASAQYSGINSQWMEALEWMGTHTPDPGVDYHAIYDQDTFTYPE
                     ESYGVMSWWDYGHWITFIAKRIPNNNPFQHGVAGPNGSATYFVSTSEEEANRVLDNVS
                     TRYVITDIDLDTGKFHAPATWANPDVGRDRFEPTFFIPVSAGSTSYQAMTFYNQQYYL
                     TMVSRLHNFDGSMTDPTSQVIYAEYRDADAANASLPVITRTEQMNATAAAAAVEAYNK
                     NAPAGSHATLLNMYYQYRGDAILQPVEQVPALQHYRLVHESPQNVFGNVGAGGPDLKS
                     VKIFEYVPGARIKGDGIIEVPVVTNTGRAFTYRQESVNGTFIVPYATSGWSGEVKTTG
                     PYRIAGTGQTFDVTEEDIRQGRTIN"
     gene            19366..20334
                     /locus_tag="PLV90_00780"
     CDS             19366..20334
                     /locus_tag="PLV90_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOF95675.1"
                     /translation="MRASVIYGDIFTRHDMEAHPESGSRLLGALAGVPENVRWHAPVR
                     ATEQDLERVHRPQYIRWVQEIARGPCFLDPNTYVTCHSFETALYAAGSTFAAVERALD
                     GEHQFALVRPPGHHAEPDQAMGFCIFNNAAVAAAKALLSVDRVAIVDWDLHHGNGTQT
                     AFYGSDRVLFCSVHEADSFPKTGWVDEIGTGAGRGYTLNAPLAAGSTIADYQHVFREI
                     FVPALVRFRPDVLIVSAGQDALSDDEHGRMNLEPEDYTALTGMLIDGTDLPLALTLEG
                     GYGPSHGKAIAAIFRALSGKRHTPAERPLHRTTERVVDILKKVRFC"
     assembly_gap    20719..20818
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(20901..22076)
                     /locus_tag="PLV90_00785"
     CDS             complement(20901..22076)
                     /locus_tag="PLV90_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-related McrA-glycine thioamidation protein"
                     /protein_id="HOF95676.1"
                     /translation="MAIMIHPVEKLYFDGTHRSRSPEETYAAVEPLMAEIGVTEIVDV
                     TPLDRLGIPVFTAVRPGAARGAARVHAGKGKEPIHARVSAMMEALERYCAEYRGDRME
                     YATYEEIGPGRAVHPEDLFVPRKLEQGEKLHWTPVWDILNDEEAYVPSNAVFHPYDSL
                     GMAVPLFRSDSNGLASGNVMEEAILHALFEVIERDALSLAEQRHDLGHRLTIGNDCAA
                     REVLDRFEENGIEIHLWLLDGKTGIPTVAAAADDTVTRDPAMIVIGSGTHACPEIAAL
                     RALTEVAQSRGSYLQGGRTDPQREMVIRKAGYERLKRINRMWFADAEAVDIRDIPDVS
                     TNRFDLDIERALQEISPYADRVCVCDLSRTPVPVVRVVVPGFEVSYMDPDRKRPAQG"
     gene            22133..22423
                     /locus_tag="PLV90_00790"
     CDS             22133..22423
                     /locus_tag="PLV90_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2098 domain-containing protein"
                     /protein_id="HOF95677.1"
                     /translation="MSTDDVVVGAIVRYPRTGTTGKVLRVEDIDGRRYAELDSTGLYY
                     RVDELVGADRTTGPVEREGHSLEDYLAEHMEFKEQLEQVWEKGIDQSCEGGG"
     gene            complement(22502..22924)
                     /gene="nikR"
                     /locus_tag="PLV90_00795"
     CDS             complement(22502..22924)
                     /gene="nikR"
                     /locus_tag="PLV90_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOF95678.1"
                     /translation="MSGDTELSRIGISLPKNLLDKFDEILSLRGYSSRSEGIRDSIRS
                     YITHYQWISDVKGERQGVITMVYDHDQRGLLQTLTEIQHQYAHIIQASLHSHVTHNRC
                     LEVILIRGDGAVLKDITERLMAQKGVEAVKLTTIPIEG"
     gene            23137..24372
                     /locus_tag="PLV90_00800"
     CDS             23137..24372
                     /locus_tag="PLV90_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     gamma"
                     /protein_id="HOF95679.1"
                     /translation="MRDAFIPGVNIGLVGHVDHGKTTLVSALTGTWTDRHSEEIKRGI
                     SIRLGYADTTFYRCERCEGAEAYTSRPECPSCGGKAVPFRTVSFVDAPGHETLMATML
                     SGSALMDGAMLVIAANEVCPQPQTKEHLMALELIGIKKIVIVQNKIDVVTQAEALEHY
                     KQIKRFIKGTIAESAPIIPVSAQKGVNIGALIQTLDSIVPEPARNPDIDPVLFIARSF
                     DINKPGCSWRDVKGGVIGGSLIRGILHEGDDIEIRPGRQVQVENRMKWEPVETKITSI
                     NAGKTRVTEAAPGGLLGVATKLDPALTKSDALAGQVAGLVGKLPPVWERLKFDVTLMD
                     RVVGADSEQIIEPLKHKEPLMLSVGTAVTVGVIVNTKKNQVEVQLKRAVCAEVGARIA
                     ISRQVGGRWRLIGMGVLVE"
     gene            24369..24749
                     /locus_tag="PLV90_00805"
     CDS             24369..24749
                     /locus_tag="PLV90_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HOF95680.1"
                     /translation="MRVLLDTNALLMPAQFGVDLYDELLALFGDFEPIALEEVVGELS
                     GLARGRGRDAAAARVGLALVRRSTVVPSGSTAEGVDNRVIEYARREGCTVVTNDRELR
                     NALLREGVDVVSMRRQKTLELLRG"
     gene            24753..25322
                     /locus_tag="PLV90_00810"
     CDS             24753..25322
                     /locus_tag="PLV90_00810"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HOF95681.1"
                     /translation="MYYKMTLEDKVRVPPHRLGEDLGAVILNVLQEQLEGSIAKEIGI
                     FIAVTKIIDIGEGELIPGDGAVYYDVRFEAAVLRLGLQEVIEGEVVETTSFGAFVSLG
                     PIDAMLHVSQISDEYINYDEKNGRLICQDSKRYIAVGDAIRARVVALSLNEREPRESK
                     IGLTMRQPGLGTSVWLEEDLEEEKQKGAA"
     gene            25324..25524
                     /gene="spt4"
                     /locus_tag="PLV90_00815"
     CDS             25324..25524
                     /gene="spt4"
                     /locus_tag="PLV90_00815"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor subunit Spt4"
                     /protein_id="HOF95682.1"
                     /translation="MVVRKKVVKVCRECHRVVEGEACVICGTANLSEDWAGYVVIIDP
                     EHSDIAKKMKITLPGRYALKVR"
     gene            25524..26012
                     /locus_tag="PLV90_00820"
     CDS             25524..26012
                     /locus_tag="PLV90_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-dependent dephospho-CoA kinase family
                     protein"
                     /protein_id="HOF95683.1"
                     /translation="MLRLPETHRDLFKKPFGTLYGSIGELLPRLSGRAVYAVGDVVTH
                     NLLDAGVIPDVAIIDGYTMRLPCTRLPLFRARRRLTAKNPAGTITDELAGAIADLVRG
                     PPGVLFVDGEEDLAVIPLVLAAPDGAAILYGQPGEGVVLRIVDEPAKREARFMLTCFV
                     RE"
     gene            26074..26367
                     /locus_tag="PLV90_00825"
     CDS             26074..26367
                     /locus_tag="PLV90_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S24e"
                     /protein_id="HOF95684.1"
                     /translation="MDFEITRDVRNEVLSRRELTFTLTFDGPTPSRKSIQEKLAAMLN
                     KNENLVVLDLERTRFGAMELSGRARIYDDEEKKKSTERGYLLKRGEPKAESEA"
     gene            26373..26528
                     /locus_tag="PLV90_00830"
     CDS             26373..26528
                     /locus_tag="PLV90_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27ae"
                     /protein_id="HOF95685.1"
                     /translation="MAVKRHECYELKGDKVVLQKRHCPRCGPGVLMAEHKDRVACGRC
                     GYTEFRK"
     gene            26610..28196
                     /locus_tag="PLV90_00835"
     CDS             26610..28196
                     /locus_tag="PLV90_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional N(6)-L-threonylcarbamoyladenine
                     synthase/serine/threonine protein kinase"
                     /protein_id="HOF95686.1"
                     /translation="MSDTRSEDGLVLGLEGTAWNLSAALFGEGLVALHSSPYIPQKGG
                     IHPREAAQHHASVMKEVVSRVLTEPERIRAIAFSQGPGLGPSLRTVATAARALAIALG
                     VPLVGVNHCVAHIEIGRWATGFADPIVLYASGANTQVLGFLNGRYRIFGETLDIGLGN
                     ALDKFARSHDLPHPGGPAIERLAREGNYIELPYTVKGMDLAFSGLVSAAQESTAPLED
                     VCAGLQETAFAMCVEVTERALAHAGKDEVLLVGGVGANARLQEMLRTMCEDRGASFAV
                     PERIYLGDNGAMIAYTGKIMLEHGVTLPIEESQIRPGYRADEVEVAWWTGPGEIFAAG
                     PLPEGGIARGAEAVVEIREGDVVKRRTGKRYRNPGLDRRLIAERTRAEARLIAAARRA
                     GVPTPVIRDVTADTIVMERVEGDVLKYVTSAENVRLAGKAVGRLHGAGIVHGDLTTSN
                     MIVRGDQCVLIDFGLASTSPEVESRGVDLHVLFQTLESTTENYGELREAFVAGYAETF
                     PGADEVLSREHEVELRGRYL"
     gene            28193..28741
                     /gene="rdgB"
                     /locus_tag="PLV90_00840"
     CDS             28193..28741
                     /gene="rdgB"
                     /locus_tag="PLV90_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOF95687.1"
                     /translation="MRLAVVTSNANKAREAAAYFAGVLEVEHVALECPEFRHVDIGEI
                     ARGKAEFAYGVLSRPLIVDDTGLFIDALGGFPGPCAAYVHDTIGNAGVLKLMEGVENR
                     GARFETAIAFAREDGIRIFRGILPGTIVAPRGIGGFGYDPIFASGEQTLAEMPLAEKN
                     RISHRARALEAFRTWITQEGGS"
     gene            28800..28946
                     /locus_tag="PLV90_00845"
     CDS             28800..28946
                     /locus_tag="PLV90_00845"
                     /inference="COORDINATES: protein motif:HMM:NF003161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L40e"
                     /protein_id="HOF95688.1"
                     /translation="MARFPEAEARLLNVKVCMRCNARNAIRATRCRKCGSEELRSKSK
                     ERKA"
     gene            complement(29042..29692)
                     /locus_tag="PLV90_00850"
     CDS             complement(29042..29692)
                     /locus_tag="PLV90_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1917 domain-containing protein"
                     /protein_id="HOF95689.1"
                     /translation="MEEIDPESLGEVAYGIFEVYLNRELRHPGSHLFELVEQGTDFTD
                     DAARVFERFGEDYPDLAGALIRRFGGIDAVYASIRAGEGILPSKTTRMYWIVQDAPGP
                     LERGLDDEQVGKWLIFVPPGEADEAWRKIRDATARGELGISAKVSTAKPNPDSRDERA
                     VMYVYTRDWADEADVMRVRKRLRDLGFTERIGYKRNIETYRGEYSEEGRKVTYYSA"
     gene            29957..30838
                     /gene="htpX"
                     /locus_tag="PLV90_00855"
     CDS             29957..30838
                     /gene="htpX"
                     /locus_tag="PLV90_00855"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HOF95690.1"
                     /translation="MKWTRDFGLTMRMLLTSFLLLIVYLIFLGVLYALGFPFEFLLVV
                     AAGMAFLQYFFSDKLVLWSTGTRIVAEDEYPELHRMIEGLAVRAGLPKPQVGVMPSPV
                     PNAFATGRSPSHAVVAVTDSIMRMLTREELEAVLAHEMAHVKNRDMLTLTMASFLSML
                     AFLIMRNWFFMSLFGGGNRDNNMGALILVYVVSIVVWLVSTLLTRALSRYREFAADRG
                     SAELTDNPRALISALKKISGRMDYIPAEKKQEVEGANAFFIIPALSGNTLMELFSTHP
                     SLEKRVAALEDLAAGAP"
     gene            31256..>31830
                     /locus_tag="PLV90_00860"
     CDS             31256..>31830
                     /locus_tag="PLV90_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95691.1"
                     /translation="MALKRVFGIFAVICILALAGPVVAVQVPEDQSQFVFPGEIREAG
                     AAVAFAKGDYLADRFPENMEVIGRYNVTIDPAEVFKLQPFNLDNPIEVKRDTPIGALD
                     AVARTGALNYSTYLYSTRNLLVIDHINGHLYQGDWLWLVVYENGSYITSTPDMWDYSI
                     SNGETIWLIYCDLTDFNSRYESRLDRAVAGLS"
BASE COUNT         6022 a        10012 c        10038 g         5658 t
ORIGIN      
        1 gggaaggcag cgcccggacc cgggcgtacc gcacgcaggg tatcggcatc tccatgctcc
       61 acaatataca ctcctggtac cgtgagcaaa tccttcccga aaaggacgag gtcgcctcga
      121 ggcgggtggg acggagtaga gaagggatca aaaacctcat tttagtgccc gttgaagaga
      181 tccgtgtcgg tggctgtggc accttctctc tatttgcgaa tcgtctctga cggcaccacc
      241 ccgcgactcg cctacgattg cgtagttgcc cgaaatcccc tgacaggaaa gaggatccgc
      301 ttgcagccct gcagggcgca aacttcagtc atcggaaaca tcgttgctcg aagaccttcg
      361 gtcttctcgt gttcgctacg ccgcgacccg tagcttcgcg gcgctcgagg tctgctccga
      421 agagtatcac aacctttatg ggatacaatg ttatatgttt ctaacataat gttagaaata
      481 aacaacatgg tgcggtgaca atgcagttga aacagtccgt acttctggcc ctcggcatcc
      541 tgctcgttct tgcaggtgtc gtccttcagt ataccggcat cgtcgtcgaa agcaccgcta
      601 cggtcgcgct catctcggcg ggtgtcgcca tggtgaccat agcggttgtc atgggccttc
      661 gcaggcagga cgaagtcatc tccgatgaaa gaacgaagag aatcggcgct tacggcacca
      721 cctactcctg ggtgctcacg ctggtactga tactggcctt cttatggctg gacttcctcg
      781 gtctggtgtc gttcaatctc cagggaacgc ttctggtcct gctgtttacc atgctcatct
      841 ccgcacggat cttccagtgg tacctcttcc ggcagggaga cgtggcgtga ggcaggcaga
      901 tgccgccgaa aggagtctct ctcggaaggg ggacgtcggg tgaagacccg gatccgggaa
      961 ctccgtgcga agacggggct cacccaggag gaactggcgc gagaggtcgg cgtgcggcgg
     1021 gagacgatcg tcttcctcga gcaggggaag tacaaccctt ccttgaagct cgcctacaag
     1081 gtggcccggg tgctcggcgc ctccatcgag gaggtcttca tgttcgagga agaggacctg
     1141 gcgtgaccgc gccggagccg tagggcggca gagaaacccc gcccgtgacg ctgccgccag
     1201 agccccggcg gtggcggagg tcgtctggcg ggcgcaaccg gcttttccgg agggccgaac
     1261 ggcaggtgag caggaaagaa ggcagagcac cgggcagacg cccccgcgac gatatgcggg
     1321 acagaagaga agtcggaaaa atatattggg ggcaattgtt aaggttgtaa tataaggtta
     1381 aaaacattcc ggtttttaat tcgcgcaaaa ttccgccaga ttcccgtaaa tggtcttata
     1441 ttattatatt tttgaaaaat acatttgtat tgtgcgataa atatattatc tcccggcaat
     1501 ttgaacattc ggggtgggcg cagcactatg gaaggttact tcggtttttc gccggatctg
     1561 cgtcgatcat ttccacccct gtcccgcatc ccccaccagg cgcctcgcct ctgcctcgag
     1621 ctccggcagg aggtcgtcac ggtttgatgg ggtcacctcg acgacccgaa cgtccgcccg
     1681 gcccctgatc cggtcgagtc ccggcatgcc ccgctgtgcg atcgtcgcca cgcagggggc
     1741 gggagcatcg agcgcctccc gtacaatccc gcgaaagacc cccgaataac actccatctt
     1801 ccctatctca tcgatgaacg ccaggcgggt gcccggtgtg aagaacggga tcgacgagag
     1861 aaaatcctcg aacccctcga tatcgacccc gtaccggccc acacggcagc gacccgggta
     1921 tccggtgcgg gcgaagggtc gccgatcgcc ggcaaggctc acgaggtcga accccacccg
     1981 cactccccct tccctgacct cgacggtgta gaacccgacc ggcgagcagt cgcgaagacg
     2041 ctctgccaga cggcgcatga gcgtggtttt cccggaaccg ggccggccgg tgatgagcag
     2101 gttcccgatc accgttccgc ggcctccccg gtgcggaccc tccggcggca gaccggcacc
     2161 atcactcgtc tcccctgagg agcgcaaaga gccgggcgag cgagacgagg tcctgccggt
     2221 tatgctccac caccggtatg agcgggccgg ggttcccggt ccgccggtag atatcgtaga
     2281 actccggcac catccagccc gggaggtcgt cctgccgctc gaccccgaga acctccgtct
     2341 caagcacgtt gagccggcag gcgggtatcg agcctttcca gcgccgccgg gcaaagagga
     2401 gcgcatcgaa gtgaggcgct ccaaggtccg ccggaatacc gtagcgtaca agccgttccc
     2461 ggatgaaggg gaggtcgaac gcccggccgt tgaaggtgac gagggccgca ccctccgctt
     2521 ccaggtcggg gagggcggtg gcgagcgccg ccgcctcctc gttgagggac cggagcaggt
     2581 actggcggat ggcgatcgcg tcaccctcta cccgggcaag cccgatgagg acgatcggcc
     2641 gggaggagag ccccagcgtc tcgatatcga ggaagacgaa cgtttcgggg gcgtggaacc
     2701 ggctgacctc gaggaggagc gggtcgctct gccggtgccg ccgcccgatc cactccataa
     2761 gaccgcaggt atccctcccc agaaccccat cgaggatccg cgcggcatcc cggcagtaga
     2821 ggggatgccg gacgagatcg gcgatcgtcc ggtaaccctg ccgctgcagc ctctgccggg
     2881 tcgcgccacc gataccgggg accagagtga gatccgcaag gatggccgct gccgcccgtt
     2941 ccgggtcacg gctatcgagc ccttccgtca tggggcttct ggattcaagg acgtagcaac
     3001 tcccggtggg gcatgcaacc tcccggccag agaaggcatc ctcgacagtc aggccgcggt
     3061 attcggcgat gaggtcgtcc cggatgtgcc gggcctgatc gcagccgggc gcgatgctct
     3121 cccgaaaaag tccgcgccca ccggcccgcc accctctccc cgcacgatcg aacggatacc
     3181 ccatcctgcc gggagggtta gatcctcccg gcattcaaag gtgatccccg cggggtgaat
     3241 gcgagcggca gggcacgtca ctcccgacct gccggcctca ccgcgatcga gcacgcgcgg
     3301 tcgccggcgc agatgctgct ccggtacttc ggcgcataat ccgggttcag ggccggtatc
     3361 gcggcgtttg cgtaggcgtg gcaggcgttg cagacggtaa gcggatcctc ccctcgctca
     3421 tctgcccttg ccaccatcgc gcacccccgg acggtcagca acgcctcgtc cgaccccgat
     3481 tcctggatct cataccgata ctcgggcccg aagaagaccc gggttgccgc cgcaaacgcc
     3541 tctgcaaccc cccgtgcgtt cccggtcggc ataccgaacg cgcgggcgat gacgcccacc
     3601 tccctgccct gttcctgcca cgccgcccgt tcgacctcgt tgtagagata gtcgtcggcg
     3661 ccgtccctga tggcccgccg gtaggcaagc ggcagcgcgg agatctcccg ggccgccagc
     3721 ttccacctca catccattgg aattcgttcg aatacatcca tacgctctcc ctccatctcg
     3781 tgtcggcctc catgccggag gtggctatac ccgcaaccca catgaacgtt cgcccgggag
     3841 gcaggcgcgc gcataaatac cccggcgagc aacaggagag catgaaggtc agagcagtcc
     3901 cgatcgatat cggtggcgcc cggacctccg ggacgaaggg aatcccggcc gggaccgaca
     3961 ggcgccggga gagggcggtt ctccggtcgg tccccgtacc ggatctcgtt gtcggcctgc
     4021 tcgccgaccc cctcgatcac ccctgatacc atggaaagga agttcctcgc cacgctcatt
     4081 ctggtcgcca tggccctcgg ggccgggtgc gcggacatcc tcccgggccc gccggttgcc
     4141 ctgccggcga tcgtgccggt cgagggggcg agccttgacc ctctcccggc ccacaccttc
     4201 ccctttgagg gcggagagga gacgatccgg atcgatcccg atccggcggt ctatgccggg
     4261 gcaaaagggg cggaccgaag gcttcatctc gcaagagacc tctcccggga agagtggatc
     4321 cccctctact accgggcgtt cgagagcgaa ccccacctgg aacctttcta tgcggacctg
     4381 ctcgcggccc tccgcgatat ccgggaccgc gaggggcttg acgacgaccg atacctcgaa
     4441 ctgatcgccg tcttcgtgca atccattcct taccggaccg acgcctcgat cgtggagccg
     4501 aagttcccca tcgagaccta tgtcgacggg gaaggcgact gtgacgacag gagcctcctc
     4561 ctcgccgggc tcctcgcacg ggagggttac ggggtcgcgc tcttctactt cgaggacgag
     4621 gcgcacatgg ccgtcggcgt gcagagcacc gggtgccact accgtaacac ctcccttgcc
     4681 tacgtcgaga ccacgaacgt gagttatgtg ggcatcccgc ccctggccct ctcgagcggg
     4741 gtggtgctcg cctcggatcc cctggttatc ccggtcggtg acgggccgcg cctctacacg
     4801 gggtgcgatc agatcgagac gatcgaacgc gccctctcct cctcccttgc ccgggccggg
     4861 gcacttgcgc ccgagctcgc ggcgcgcacg cgggatatcg aagcggagcg ggagtctctc
     4921 gaggccttcg gcacgcggtt gaaggctctc tcccgggacg gggagttttt ggagtacaac
     4981 cgtctcgtcc cggaatacaa ccggaaaacc cgggaataca acgacgcggt gcaggcctac
     5041 aacgccctgc tcgcggagtc gagggcagcc gtagaccttc acaaccatct cgtcacccat
     5101 gcctgcgacc ggcccgggtc gtacctcagg gcccgggcgt acctcgcggg atgatgcacg
     5161 aaggtcggtg gagcgggggc cgggcgagag gggccttgca agtaccccgg agaggtctca
     5221 agcgcgcccc tctgctttga ggggagtgcg atccgcgggg gcagcgatgc tggcatacgc
     5281 tgctttacac atctggctcc gccggacaat ggtcgggtat cgggctgccc gctcggcgag
     5341 gcaggcaaac gttgcaaacg cagtctcttc catacaaaga acaagggcga ggcctctatt
     5401 taaccaggag gcgtgcgggg tgaaagtgaa agtcaggggc aacggtcgtc tctcctgtct
     5461 cgtttgcgcg ttaagccgcg gcaccggcag gcgttcatga aaaaatccct gtatcgagcg
     5521 ggtttgcccc tacaggggca gaaacggcaa ctgttccgtt ggcaagtgtt aatgtatccc
     5581 cttgaccaat acatgaaacg gagtgccgtt atgctggata tcagtgatct gcacgtgaac
     5641 gtggatgaca ccgaagtgct ccacgatatc aacctccaca tcgggcaggg ggagactcat
     5701 gttctgatgg gcccgaacgg ttcggggaag agcacgctgc tcaaagccgt catggggttc
     5761 ggcggctaca cgatcacggc cggatccatc gtattcaggg gaacagatat cacccacatg
     5821 ccgatccacg aacgggctca tctcgggatc ggcatgatgt ttcagcaccc gcctgctata
     5881 tccgggctga aactcgggaa actgctcacc gccacgtctc acatgggaga gggcgcgata
     5941 gaggcgctcg cgcagtcggt caacatggag cgtttcctta cccgggacat caacgtcggg
     6001 ttctccggcg gcgagataaa acggagcgag gttctgcaac tgaaggtcca gcagcccgac
     6061 ttcatcatgc tggacgaacc ggagagcggc gtcgacctcg agaacatgaa cctgatgggc
     6121 aaggagatcg caggcctgct cgagaaggat gtccatatcg tcaaccggcg ccggagcggc
     6181 ctgatcatca cccacaccgg ctacatcctc gactacctcg aggccgacca gggccacgtg
     6241 atgatcagcg gccggatacg gtgccacgga aacccccgcg agatcctgcg cgtggtcaaa
     6301 gagaagggat acggagagtg cctgcgttgc aaacagatat aaccgatatg gagcgcctct
     6361 cgcaggagga ccgggaacgc ctcgccctga ccggccttga ggtcggtatg cagaaccggt
     6421 gcgggagctt cttccagatc gaccagaagg tgatccagac cacctgcggg gccgagggga
     6481 tcgagatgct ccccatccgg gccgccctcg agaagtacga ctgggtgaaa gactactact
     6541 ggaacgccgt ctcgaaggac aaggataaat acactcagtt cgtggcaaaa caggagaacc
     6601 cgcagggtct cgtcgtcatc gctcatgagg gtgcaaaggt cgcgatgccc ctccaggcct
     6661 gtctcttcct ccgcgacgaa ccggtgcagc acgtccataa catattcatc gcaagggagg
     6721 gctcggagat gcacatcatc tccggctgcg cgagttcctg gcaaaaagag cacggtgcgc
     6781 atatcggcgt gaccgagatc tacgtcggca agggcgcgaa agtcacgtcc acgatgatcc
     6841 acaactggaa cccggggatc agcgtcttcc cgcggagcgc caccatcatc gaggagggcg
     6901 gcgtcttcct ctcaaactac gtctgcatgc agccggtcaa tcgggtcgac atgtacccga
     6961 cggcgcgcct ggtcggaaag aacgccgtgg cccggttctc gagcatcgtc gtcgcgaccc
     7021 cgggctcgat cctcgacctc ggctcccggg ccatcctcga cgccgaacag acgagcgccg
     7081 agctcgtcac ccgtgcgatc accaccggcg gcaaggtcat ctcccgcggg cacatcctcg
     7141 gcgaaaagga cggcacacgg ggccacatcg agtgcaaggg gctgatcctg aaagacggca
     7201 tcatccacgc catccccgag atcgagggga cgctgaccgg caccgaactc tcccacgagg
     7261 ccgcggtcgg caagatcgcc cgccacgaga tcgagtacct catggcgcgc ggtctctcgg
     7321 aggaggaggc caccgcaacc attatacgcg ggttcctgga tgtcaagatc agcggcctgc
     7381 ccgaagttct gcagaagcag atcgatgccg cgatagataa ggcagaatca ggattctgac
     7441 ggcaaagaga ggaggagatg acaaagaccg acccctacga gagagagcgt gaagcgatgg
     7501 tggagcacca gatccgggac cgcgggatca gggacgaacg ggtgctcgcc gcgatgcgag
     7561 aggttccgcg ccatctcttc gtgccgaagg gctatgagcg cgccgcctac gaggaccggc
     7621 cgctccccat cggggagggg cagaccatct cccagcccta catcgtcgcc gtcatgaccg
     7681 agcagcttgg gcttgcaccg caggaccggg tgctcgagat cgggagcggg agcggctacc
     7741 aggcggcgct cctcgccggg ctcgccggga cggtcatatc gatcgaacgc ctggagggcg
     7801 tcgccgaaca ggcacgcgag aaccttgccc gggcaggggt caccggcgtc cggatcatcg
     7861 tcggcgacgg cacggaaggt taccctcccg aagcgccgta cgacgccatc atcgtcacgg
     7921 cggcgtcgcc cggagtcccc gaacccctga tcgagcagct tgccgaggga ggacggttga
     7981 tcgcccctat cggcccgcga gactgccagg acctcgtcaa actcgtgaag agggagggga
     8041 aggtggagac gatcccgctc ggcggcgtct gcttcgtgcc gctgatcggg cagttcggat
     8101 ggcgaggaga agggttccgg tgatcatcta cgacatcacc cgcgacctct cggcggatgc
     8161 catcctctac ccgggtgata tccggccccg atttcgcgag atcgataacg ggcaataccg
     8221 cgtgacggag atgaccatcg ggacgcattc ggggacacat atcgacgcgc cgtcgcacta
     8281 catcgagggg ggcctgacgg tcgaccggat cgcgctcggt gtccttgcgg gttcggcacg
     8341 ggtaatcgac tgcagcgatg cggagacgct catcggcccg gagaacctcg ccgaccgcct
     8401 cgcgggcgcc cggacgctcc tcctgaagac atggttctcc gggcgggagg agttcgaccc
     8461 cgggtacccg gcgctctccc ctgaagcggc ggacctcgtc gtcgaggccg ggatcacctg
     8521 tatcgggacc gacgcgccat cgatcgaggc gttttgtggc gacggttcgg tccaccgccg
     8581 gttgctcgga gccgggacgg tcgtcctcga actgctcgac cttgccgccg tgccggaagg
     8641 agagtatcac atggtggcgc ttccgatgcg tcttgccggc gccgacggtt cgccggtgcg
     8701 ggcgatcctc tgtaacggga aggagtagca tgagttttct caacgatgca atcaggagac
     8761 ttgagcatat cctcgcagac agcgggtgcg agaacggtat cgtcgacctc tctgtgaact
     8821 acaacgcgga tgtctgccag tatccgaagg gcgtctgcat ggaggggcac tttggcggac
     8881 gaaccggcca gttcgtcacg agcgaaccga tccgggccag aacccggatc tccttcatgt
     8941 tcggcgcgcc gctcacggac cagaagcagc gcggggctgc aagcgcgatc atcaacgccg
     9001 tctcggcgtt cctctgtctc gcgcgaaaac tccgcccctg cacccccgac tgctacgctc
     9061 cctgccttgc cgacctctcc cgcgagatcg gcagaaagcg catctaccct gtcggcccga
     9121 tgccggtgat cgagcgggcc ttcaaggacc agatcgtcga ttctccggaa gaggccgata
     9181 tcctcctggt tgccggcgaa ggtatgacga cggacgccgg aatcgcctgc atcgacggct
     9241 gccgggggaa taaacggata atctttctcg ggccgtcgac cgcaggggtc agtggcctcc
     9301 tcaatctcga gcactggtgt ccctacggtc gatgaatacc tttttttagg aaagaaggga
     9361 ttatacaggg gtatgctatt cggctcttcc ggaatccgtc aggagttcgg tcccggcctc
     9421 gtcgacctcg cgctccgcat cggagccgct gccgccgacg gcgcatcgcc cctcgtcgtc
     9481 ggcagggata cccgcacgac aagcgaactg ctggaacatt gcgtcgtcgc cggcatgctc
     9541 tcggccggtg cagccgtcta cacctgcggc attgcgccca caccgacggt cgcctatgcg
     9601 accaggcgcg cggacgcagg atgcatgatc accgcctcgc ataaccccga gccctacaac
     9661 ggggtcaaac tgctcaaccc tgacgggtcg tcgttcaccc gccggcagca ggcggcgatt
     9721 gaagaggcgc tcgaagggac gcaccgggtg gggtgggaag aacaggggca cacctctccc
     9781 atcgatgcgc ttgccatgca ccgggatgcg atcctcggcg agatcagcct ctcccggccg
     9841 gtcacggtcg ttctggactg cggcaacggt gcggggagcg tcatcacccc cgatctcctc
     9901 agcgatgcag gggcgaccgt cgtcggtctg aactgcaaca tctcgggccg atttgccagg
     9961 ccgtcggaac cacttgaggc aaaccttccc tacgtcggcg agatcgtccg gaagcgaggg
    10021 gccgggtgcg cggtgatcca cgacggcgac gccgaccgta tgatggcgtt cgatgaacgg
    10081 ggccgctata tcgacggaga tcatctcttg atgctctttg cagcatacct cgaccacaag
    10141 cgggtggtca ccaccgtcga cgcctcgatg gcgatcgagg aggtagccga ggtccgccgc
    10201 acaccggtcg gcgacacctt cgtctccgaa gagctcctca cctggggtga ttttggtgga
    10261 gaggcgtcgg ggagctggat ctttcccggg cactcctact gcccggacgg agtctacgcc
    10321 gcggcgctcc tctgcgagat cgcatcggaa tggtcgatcg cggaggagct ggaccggatg
    10381 ccccggtaca ccctcctccg ggagtcctgc cgcgtcgcct cacccgggga gatcatggcg
    10441 gcgatcggcg ccgacgaacc gacgcacggc gtccggttca gtgatgacga cggttggtac
    10501 ctgatccggg caagcggcac cgaaccgaag gtccggataa cggccgaagg aaagacgccg
    10561 gagaaagcaa aagagatgct cggtgcaggg catgccgccc ttgcgagagc aaaacgtatt
    10621 ctcggaggaa gagaggagtg agtagtatgc aatgtgtcgt tctggctgca ggcgagggga
    10681 aacggatgcg ccccctgacc gcccggcgcc cgaaggtgat gcttcccatc gccaaccgcc
    10741 cgataatgga acacctggtc gtcgcggcca gggatgccgg gatcaccgac ttcgtcttcg
    10801 tcgtcggcta cttcgagcgt gagatccgga accacttcgg ggatgggagc agcctcggcg
    10861 tgaagatcac ctacgtcacc cagcgccacc agctcgggac cgcagatgcc ctccgggcga
    10921 cggcggggat ggtcaacggc cggttcctcc tcctcaacgg cgatatggtc ctcaagagga
    10981 gcgatatcga gaggttctgc cggatgagcg ttccctgcat ggggatccac gagacggatc
    11041 acccagagga ctacggcgtg gtgaccgtgg aagggggccg catcaccggt cttgaagaga
    11101 agtcggagcg cccgaagagc aacctgatca acgcgggcgc ctaccttttt gaccccgaca
    11161 tattcgacct ccttgccgga gtcaaaatat cgggccgggg cgagttcgag ctgaccgacg
    11221 cgctcgaatc ctacatccgg gaggggacgc tcgccgcgca tccgctggac tactggctcg
    11281 acgtcgggca gccgtgggat ctcctcgatg caaacgaggc gctcctctct tcgctatgct
    11341 gcgaatggcg cggcactatc gaggacggat gcaccgtccc cgatacggtc agcatcggtg
    11401 aaggcaccat catccgggcc gggacttaca tcgaggggca ctgtgtcatc ggcgagaact
    11461 gcgtcatcgg ccctcatgcc tacatccgtg gctcgaccgc cataggcgat ggctgccacg
    11521 tcgggcacgc aaccgagatc aaaaactcta tcgtcatggc aaggacgaac atcccgcact
    11581 tcaactacgt gggcgacagc atcgtcggga gcgggtgcaa cttcggcgcc ggcaccaagg
    11641 ttgcaaacct ccggcacgac aacgcggcgg tgaaggtctg cggaaaatcg accggtcgga
    11701 ggaagtttgg cgccatcatc ggcgataacg tcctcttcgg gatcaactgc tcggtcaacg
    11761 tcgggagcct catcggcagc aacacacggg tagcccccca ctccctcgtg gaggggtgca
    11821 tcgaggaagg atcagtcatc aggtgatatg atgcaggcag tcattctggc agcgggagag
    11881 gggagccgcc tccggccgct cacccggagc aaacccaaat ccatgctccc ggttgcaaac
    11941 cggccgatca tcgagtacgt catcgacgca ctactggaga acggcatccg cgacatcatc
    12001 gtggtggtcg ggtatcgtaa agaggacgtc atccgacacc tcaacaggct cgacgccccg
    12061 atccaggtcg tcgtgcagga acggcagctc gggaccgcag atgccctccg ggcggccgag
    12121 tcggagatca cgggcgattt cctcgtcctc ccgggcgaca actacgtcaa cgcggagtcg
    12181 atcgcccgga tcaggaagga acgaaacgcc atgctcgtgg ccgagcaccc gagcccctcg
    12241 aacttcgggg tcgtggtgat aagaaacggc ctcgtccgtg agatcatcga gaaacccgag
    12301 gatgccccga cgttcacggt atcgaccggg atctactcgt tctcgcccga cgtcttctcc
    12361 tgcctccgga caaacgagat ccccgatgcc cttgccgcga tgatcgcggc aggccaccgg
    12421 atacgcgcga tacctgctga cgactggcag gacgccatct atccctggga cctcctgaag
    12481 ttgaacaacc ggatgctcag ggaaatctca cctgaggtag gcgggacggt cgatgcgtcc
    12541 gtcatccgcc ggggcacggt gcgtatcggc accgggacga ccgtcgggcc gaacaccgtg
    12601 atctacggcc cggtcgccat cggagacaac tgcaacatcg gctcaaactg tgtcatcatg
    12661 ccgaacgcaa gcatcggcga ccgggtggag atcgagccgt tcacctacgt tgccgactcg
    12721 ctcatcatga gcgacgcgcg gatcggatcc cactcgagga tcgtctcggc ggtcttcggc
    12781 cagggatgca tccttgccga tcataccacc acctacccat ccgcaagctt catcgaggtc
    12841 ggagaacggg tccagaagga ggaattcggc gcagtcctcg gtgacggggt ccgcgcggca
    12901 cccttcacaa catttaaaaa ctgtattgcc ggtaacaatg ttaccatcga gcggggaaag
    12961 acagtggtcg gcctcatcga ggacggcacc cgggtgatgt agatgtgtgg gattgtcggc
    13021 tacatcggaa gacgggacgc aacgggggtc ctgatccagg ggttgaagcg gcttgagtac
    13081 cgtggctacg actcgttcgg gcttgcgacg gtcggaagcg cgatcgaggt ttacaagaag
    13141 gtgggccgga tatccgacgg ggaagcggga gcgaccggtc ttgccggata cgccggtatc
    13201 gggcataccc ggtgggccac tcacggggag ccaaacgata tcaacgccca cccccatacc
    13261 gactgcacgg gaaggatcgc cctggtgcat aacggggtca tcgagaacta cagcgaactg
    13321 aagcggcagc tcgagggacg gggccacgta ttccgatccg agaccgacac cgaggtgatc
    13381 gcccacctca tcgaggagca gtacgacggg gacctcctcg cggcagtcag cgcgatcctt
    13441 ccccggctcg agggttcgta tgccgtcctc gtgatcgcag ccgatacgca gaagatcgtc
    13501 gccgcaagga acgcaagccc tctcgtcctc ggtgtcggcg acgcggaggt cttcgccgcc
    13561 tcggatatga cgccgctcct cgagtacacc gagcgcgtgg tttatctcga ggacggcgac
    13621 gtcgccgaca tcacacccga ccgctatacc atctactacg acggccggga ggtggagcgc
    13681 ccgatcgagc tgatcagctg gtgtgtcgag gatacccgga agggcgggtt tgcccactat
    13741 atgttgaaag agatcttcga acagccccag tccttctacg agaccatccg ggcgggcatc
    13801 gacgaccacg tgcggcagat ggttatggag gccgacgaga tcacgctggt cgcgtgcggg
    13861 acatcctacc acaccgccct tgtcttcaag tacctggccg aaccgctctg taacctgccg
    13921 atccgggtcg agatgggctc cgagttcaag tacttcaccc cgccccttca cggcctcgtg
    13981 atcgcggtct cgcagtcggg ggagaccgcg gatacgatcg ccgccctcaa gatggcgaaa
    14041 gcccgcaact gccccacgct tgcgatcacc aacgtgcagg ggagcacggt cacccggatc
    14101 gcggatgaga ccctcctgat gcgggccggc cccgagatcg gcgtcgccgc aaccaaatcc
    14161 tacacggcgc agcttgccgc gctgatgcag atcgtcaacg cacgctgcga tgatgcgttc
    14221 gacgacgtcc tgtcgcacgc acatctcgcc atcagcgagg tcctcatccg cgagatcagg
    14281 gaggcggtcg ccctctgtgc gaaggcggag cacgtcttct tcgtcgggag agggccgttc
    14341 tacccggtct cgatggaggg cgccctgaag atgaaggaga tcagctacgt ccatgccgaa
    14401 gggtatgcag cgggagaact caagcacggg ccgtttgccc tgctcacccc cgagacaccg
    14461 gtggtcgcca tatgcacccc gggaccgacc tacagcgtga tggcctcgaa catcaaggag
    14521 atgaaggcgc ggagagcgcc ggtcatcgcg ctcggggtgg acggcgacaa agaacttgcc
    14581 gagatcgtgg acatattcat ccctataccg aacacacacc ttctggtgca gatcctgacc
    14641 atatcggtca ccctccagct gctcgcctac cacactgcgt gcgccctgca gcgggacgtc
    14701 gacaaacccc ggaacctggc aaagagcgtg actgtcgaat gatcgagtat ggacgtaacg
    14761 ccctcggcga gggggcgacg atatttgagc cggtgaccat cggatttccc tcccgcgacc
    14821 ggatgggaga ggaagactac ccgggcgccg ccatcgggag gaacgcgatc ctccggtcgg
    14881 gcaccatcat ctactgcgac gtcgtgatcg gcgacgcctt ccagaccggc cataacgtgc
    14941 tgatccgcga gaagaccacc atcggcgacc gcgtcgcgat agggaccgcg gcggtggtcg
    15001 agggggactg cacgatcggc gacgatgtcc gcctccagag cctggtctac atcccgaccg
    15061 gcgcccggat cggtaaccgg gtcttcatcg gcccgaacgc cgtgctgacg aacgaccgct
    15121 acccgcccgg tccccgcgag ggtctccgcg ggccggtgat cggggacgat gccgtcatcg
    15181 gtgcgaacgc gacggtcctg cccggtgttg tcgtcggcga gggggcgttc gtcgccgcgg
    15241 gcgcggtggt gacgaaggat gtcccgccca cgatgcttgc ggtgggggcg ccggcacggt
    15301 tccggccgct gccctcgggg gcaaagcggt gatgcgtgat attcctgccg gccggcagcc
    15361 tgcttgtgag agcgaacgcg tttcctgcac gatatccgat gatatcgcga ttccggccgt
    15421 gaacctccca tccacccgcc ggtgacggca ttcatctgcg atccaggcca tataacccga
    15481 tacccatgaa gatcctcctc gtctccaccc aggactacat ccaccacccg atcccctccc
    15541 ggcaccacaa catattcgag gagctcgcga cacgacacga ggtccacgtc ccccacttcc
    15601 acgtcagccg gggaaaggag cgaaaaaccc ttcttcacgt tcacgaggcg acgcggtttg
    15661 ccgtcgagag ccccttcctc cactacacct tgaacgcgcc ctgccactac cgggtcatcc
    15721 gcgacatcat ccgcgaccac gagatcgacg tcgtcgtcgg cgcccacgtc ctcgccggga
    15781 cggcgatggt ccgtgcggca gagaagtccg gcgtgccggt gatcttcgac ctcaaagact
    15841 ggttccccga ctccgccgcc gcctactaca agaacccggc catgcagtgg ctgctccggg
    15901 aaggcgtcct cgccatcacc cgctacaacc tcgaccacag cgacgtcatc acgacggtct
    15961 cgcccgggct tgtcgagaaa ctccggacct acggctacga ggcggaactg atcacgaacg
    16021 gcgtcaacac tgaactcttc cgcccgatgg atggcaggaa gatgcgctcc accctcggga
    16081 tcgccccgga tgccttcgtg ctcgggtttg caggcgccgt cgagcggtgg tatgccctcg
    16141 acgaggtgat ccgggccctt cccgatatcc tcgcgcggca ttcgaacgca gagctcctga
    16201 tcgtcggcgg ttcgctcttc accggctacc tggatgaact gcgcaccctc gcggctgacc
    16261 tcggcattgc ccggcgggtg cacttcaccg gcgccgtcga ctactgcgac ctccccggct
    16321 acatcgcgcc gatggacctc tgcctcatac cgctctcccc tccgcagtgg gtcgatatcg
    16381 ctctccccaa caagtacttc gagtactcgg cctgcgggag accgatcctc tccaccccca
    16441 tccccgacat gctccggatg ggcggcggca gcatcgccgt ctaccgggac aagggggagt
    16501 tcatcgagaa ggtcgatgag cttgccggga atcccgggcg ctgcccgctg gggatcgagg
    16561 agcacagctg gaagaagaaa gcggaggcat tcgagaaggt ctttgcacgg gtgatgaaga
    16621 aagcctgata aacgcttctt ttttctgctc tcccggatac gccaagatta ttaatggttc
    16681 aaagggaaac agaaacgtag caaggagggt ccgatggctc acgtggatct cgataagtat
    16741 cgaccgtata tcatcattgg tgttatcgtt ctgctcaccc tgctccctct ctgggtacgg
    16801 ggtttcattc cggcagccga catggtgacc cccgacgggg tcaacctcct cggcaacgac
    16861 ccctggtaca acgtccgtca ggtcgaacag accgtcgcga acttccccgg ttacgcctgg
    16921 ttcgacgcca tgacgctcta cccgagcggc gacgtcgtct actgggggcc gctcttcatc
    16981 cagatcatat cggcgctctg cgtgctcgtc ggggcaacga cgcgccccga gatcatggtg
    17041 gtggcatcct gggtcccgcc gctgatgggc gcggcgatgg tgccggtcac ctacctgctc
    17101 gcgaagagac tcaccaactg gaagaccggt ctcatcgcgg ccggcctcat cgcggtcgtc
    17161 tccgggagct ttgcccaccg ctccctcttc gccttcgtcg accaccacat cgcagagacc
    17221 ctcttctcga cgatcttcgt cctcgcctac gtcgtggctc tgcttgcctc ccgcgacata
    17281 tcgctctccc ggaggagctc tgaggtcctg aagatgccgg tgataacggc ggcgttcgcc
    17341 ggtgtcgcct acctcatcgg tttcttcaac atgccgacga tgatcctcct cgcgcttatc
    17401 gtcgcgatct tcaccctggt ccagttcgtc ctggactttg ttcaggaccg gccgagcgat
    17461 tacctggtcc tcgtgaacgc cgtgacgttc ggcgtggtga tcatcgggac ggcggcgttt
    17521 ggcttcccgc ataccggcct cgacctctcc cgctacactg tcgggcacgt cgtcgcctgt
    17581 gccggcctca tcggcgggac gctcgtgctc tacgggctct cggtctacct ccggggacgc
    17641 ccgaagtact acttcccggc agcccttgcg ggcctcgggg tcgtcgccgt cgcagtgctc
    17701 tccgtcgccc tgccggatat ctacaacctc ctcatatcga gcctcttcgc cttcttcggg
    17761 gaagcgcccg tcaccacgac cgtccaggaa gcgcgggcct ggtcgtttga cgcggcctgg
    17821 gcgacgttcc actggggtct cgtgctcgcg gccggcggcg ccgccaccct gatctggtgg
    17881 agccgcaaaa aggtcaaccc ggcccacgtc ttcatcctcg tctggacggc gatcatcctc
    17941 gcatcgacgg ctgcgcacgt ccgttacgag tactacctcg cggcaaacat cgctcttctc
    18001 gccgcgatct ttgccggggc gatcctcgac gtaacctaca aagacgtcac gcgcctcttt
    18061 gggacgaaga gcagcaccgc cccgcccgat gccgaagttg ccaggaaaga ggagaaacct
    18121 gcacgaaagg ggaagaaggg agcgagggcc ccggatgccc gaaaaacgaa agtctcatcg
    18181 aaagatcagc cggactacat caaggtcgga gcctttgccg cggtcgtcgt cgtcacgctc
    18241 gtcttcgccg gaacctccct ccagaccaac ttcgcctatg ccgcaagcgc gcagtacagc
    18301 ggcatcaact cgcagtggat ggaggccctg gagtggatgg gcacacacac acccgatccc
    18361 ggtgtcgact accatgccat ctacgaccag gacacgttca cctaccctga agaatcctac
    18421 ggcgtcatgt cctggtggga ctacgggcac tggatcacct tcatagccaa acggatcccg
    18481 aacaacaacc ccttccagca cggggtcgcc ggaccgaacg gatcggcgac ctacttcgtc
    18541 tccacatccg aagaggaggc gaaccgggtt ctcgacaacg tcagcacccg gtacgtcatc
    18601 accgatatcg atctcgatac cggaaagttc cacgcaccgg cgacgtgggc caaccccgat
    18661 gtggggagag accggtttga gccgacgttc ttcatccccg tgagtgcggg ttcgacgagc
    18721 taccaggcga tgacgttcta caaccagcag tactacctga cgatggtctc gcgcctccat
    18781 aacttcgacg ggtccatgac cgacccgacg tcgcaggtga tctacgccga gtaccgcgat
    18841 gcggatgcgg caaacgcctc cctcccggtc atcacgcgca ccgagcagat gaacgccacc
    18901 gctgcggcgg cggcggtcga ggcttacaac aagaacgcac cggcgggttc tcacgcgacg
    18961 ctcctgaaca tgtactacca gtacagggga gacgcgatcc ttcagccggt cgagcaggtg
    19021 ccggcgctcc agcactaccg gctcgtccat gagtcgcccc agaacgtctt tggtaacgtc
    19081 ggcgcaggcg ggcctgacct caagtccgtc aagatattcg agtacgtccc gggcgcccgg
    19141 atcaagggcg acgggatcat cgaggtgccg gtcgtgacga ataccggtcg cgcgttcacc
    19201 taccggcagg agagcgtgaa cggcacgttc atcgtcccgt acgcgacgtc cgggtggtcg
    19261 ggcgaggtga agacgaccgg accgtaccgg atcgcgggca ccgggcagac gtttgacgtc
    19321 accgaagagg atatccgaca gggacgcacc atcaactaaa cagggatgcg agcttcggtc
    19381 atatacggtg acatctttac ccggcacgac atggaggcgc accccgagtc gggctcccgg
    19441 ttgctggggg ccctcgccgg ggtacccgag aatgtgcggt ggcacgcccc ggtccgcgct
    19501 accgagcagg atctggagcg ggttcaccgc ccccagtata tccggtgggt gcaggagatc
    19561 gcgcgaggac cctgtttttt agacccgaac acctacgtca cctgccactc tttcgagaca
    19621 gcgttgtacg ccgccgggtc gacgtttgca gccgtggagc gggcacttga cggcgagcac
    19681 cagtttgccc tggttcgccc gccgggccac cacgccgagc cagaccaggc tatggggttc
    19741 tgcatcttca acaatgccgc cgtcgctgcg gcaaaagcgc tcctgtcggt cgaccgggtc
    19801 gccatcgtgg actgggatct gcaccacggc aacggcacgc agacggcttt ttacgggagc
    19861 gaccgggtac tcttctgttc ggtgcacgag gccgatagtt tcccgaagac cgggtgggtg
    19921 gacgagatcg gcaccggcgc cggccggggc tacaccctga acgccccgct cgcggcaggg
    19981 tccaccatcg ccgactacca gcatgtcttt cgcgagatct tcgtcccggc gctcgtccgg
    20041 ttccggcccg acgtcctgat cgtatcggcc gggcaggacg ccctctccga cgacgaacac
    20101 gggcggatga acctcgaacc tgaggattac accgcgctca ccgggatgct gatcgacggc
    20161 acggatctcc ctctcgccct gaccctcgag ggcggatacg gtccgtcgca cgggaaggcg
    20221 atcgccgcga tattccgcgc actctcggga aaacggcata ctcctgcaga gagacccctg
    20281 caccggacca cggagagagt cgtcgatatc ttaaaaaagg tccggttctg ctgagggtaa
    20341 cccgcgcggc cgacacgcgc aacagcggtg ataccatctt acacgttgca gcgaaggtgg
    20401 ggactcccgc ccggcgctct cgccgggacg agggcccaag gcttgcaggt tatcacaatc
    20461 gcgctcctgt tcgtcacgat tgcaacgatg ccccattgcc ggcactatcg atggagggag
    20521 tatcgttctt ctccccggtg ccggggagtt tgcagccttc ggggcatggt ttttcgccgg
    20581 ctatcgccat gaccgcccca ttccccggag ggagcggggt tgccggagag gtatatcgat
    20641 gtcctttcac aggccggtcc gggaggggga tgggtatgca tgagcggcct ccctgtggga
    20701 gagcccgcgc ccctctccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag
    20821 ggggatgggt atgcatgagc ggcctccctg tgggagagcc cgcgcccctc tcccggttca
    20881 ttcccccccg ggggaacctc tcacccctgc gcggggcgtt tccggtcggg gtccatgtag
    20941 gagacctcaa acccggggac gacgacccgc accaccggca ccggggttct tgagagatcg
    21001 cagacgcaga cccggtccgc ataggggctg atctcttgaa gcgcccgctc gatatcgagg
    21061 tcgaaccggt tggtgctcac gtcggggata tccctgatat cgacggcctc tgcatcggca
    21121 aaccacatcc ggttgatccg cttcagccgt tcgtaacccg ccttcctgat gaccatctcc
    21181 cgctgagggt cggtgcggcc gccctggaga taactcccgc ggctctgggc aacctcggtc
    21241 agggcgcgga gcgccgcgat ctccgggcag gcatgcgtcc ccgacccgat gacgatcatc
    21301 gccggatccc tcgtgacggt atcgtccgca gcggccgcga cggtggggat gccggtcttc
    21361 ccgtcgagca accagaggtg gatctcgata ccgttctcct cgaaccggtc gagaacttcc
    21421 cgtgcggcgc aatcgttccc gatggtgaga cggtggccga ggtcatgcct ctgctcggcg
    21481 aggctgagcg catcccgttc gatcacctca aagagggcgt gcaggatcgc ctcttccatg
    21541 acgtttcccg atgcaagccc gtttgaatcg ctccggaaga gcggcacggc cataccgagc
    21601 gagtcgtagg gatggaagac ggcgttgctc gggacatagg cctcctcgtc gttcagtata
    21661 tcccataccg gcgtccagtg gagcttctcc ccctgttcga gtttgcgcgg gacgaataga
    21721 tcctcgggat gtacggcccg cccggggccg atctcctcgt aggttgcata ctccatccgg
    21781 tcaccccggt actcggcaca gtaccgctcg agtgcctcca tcatcgccga gaccctggcg
    21841 tggatgggct ccttcccctt ccccgcgtgg acgcgggctg ccccgcgggc tgctcccggc
    21901 cggaccgccg taaagaccgg gatcccgagg cggtcaagag gggtcacgtc gacgatctcc
    21961 gtcacgccga tctctgccat caacggttct acagcggcat aggtctcctc gggagacctg
    22021 gagcggtgcg ttccgtcaaa gtagagtttc tctaccgggt gaatcatgat tgccatttaa
    22081 agtaccttgg aggtgatacc ttaatagtat ggcaggtata ccctacccac gtatgagcac
    22141 ggatgatgtg gtggtgggcg caatcgttcg gtacccccgc accggcacga cgggaaaggt
    22201 tctccgggtc gaggatatcg acggccggcg gtatgccgaa ctcgacagca ccggactcta
    22261 ttaccgggtt gacgagctcg tcggcgccga ccgcacgacc ggacccgtgg agagggaggg
    22321 gcactccctg gaggattacc tcgcggagca catggagttc aaagagcaac tggaacaggt
    22381 gtgggagaaa gggatcgacc agagctgtga aggcggcggg taaaccgccc ttcatcaccg
    22441 ttgtttgatc agggcgtgaa atctccggca tcaaccgggg ggcagttggt ccgcgggggg
    22501 gttacccctc gatcggtatg gtggtgagtt tcaccgcctc gacgcccttc tgcgccatca
    22561 gtcgttccgt aatatctttc agcaccgctc catcaccgcg gatcaggatg acctcaaggc
    22621 acctgttgtg ggtcacgtgc gagtgaaggg atgcctggat gatatgggca tactggtgct
    22681 ggatctcggt gagcgtctga agcagcccgc gctggtcatg gtcgtagacc atggtgatga
    22741 cgccctgacg ctcccctttc acatcggaga tccactggta atgagtgatg taactccgta
    22801 ttgagtcccg tatcccttcc gaacgggagg aatacccccg gaggctcagg atctcgtcga
    22861 atttatcaag aaggttcttg ggtagagaga tcccgatacg tgaaagctcg gtatcacctg
    22921 acatactgta accagttttc atatctctcc tggtaaggta taaatttttg ctcacattcc
    22981 ggcgcattat tcatacttcg tatgccccga aaaaggcagc aaaatagggg gccgcccgga
    23041 tgtgcgataa gaatgcaacc ggggccggag agatgatcct caccgattat gtataagtag
    23101 tgtcactcta tattggataa ggaggttcga aaacttttgc gagatgcatt cattcccggt
    23161 gtcaatattg ggctcgtggg tcatgtcgat cacggcaaga ccaccctggt cagcgcgctg
    23221 accggtacct ggactgaccg gcacagcgag gagatcaagc gcggcatctc catccggctc
    23281 ggttacgctg atacgacttt ttaccggtgt gagagatgtg agggggcgga ggcctacacc
    23341 tcccggcccg agtgcccgag ttgcggggga aaagcggtcc cgttccggac ggtctcgttc
    23401 gtcgatgctc ccggccacga gacgctgatg gcgacgatgc tctccggttc cgcgctgatg
    23461 gatggcgcga tgctcgtcat cgctgcaaac gaggtctgcc cgcagcccca gaccaaggag
    23521 cacctgatgg cgctcgagct gatcggtatc aagaagatcg tcatcgtcca gaacaagatc
    23581 gatgtggtca cccaggccga ggcgctcgag cactacaagc agatcaaacg gttcatcaaa
    23641 ggcacgatcg ccgagagcgc gcccatcatc cccgtctccg cgcagaaagg ggtcaatatc
    23701 ggcgccctga tccagaccct tgattcgatc gtcccggaac cagcccggaa cccggatatc
    23761 gatccggtgc tgttcatcgc acggtcgttt gatatcaaca aaccgggctg cagctggcgg
    23821 gacgtgaagg gcggcgtcat cgggggatcg ctcatccgcg gcatcctgca cgagggtgac
    23881 gatatcgaga tccggcccgg ccggcaggtc caggtggaga accggatgaa gtgggagccc
    23941 gtcgagacaa agatcacctc catcaacgcc ggcaagaccc gggtgaccga ggccgcgccc
    24001 gggggcctcc ttggcgttgc gacgaaactc gatccggcgc tgacgaagag cgacgccctc
    24061 gccgggcagg tggccgggct cgtggggaag ctgccgccgg tatgggagcg gctgaagttc
    24121 gatgtcacgc tcatggaccg ggtggtcggt gcggatagcg agcagatcat cgagcccctg
    24181 aagcataaag aaccgttgat gctctcggtc ggcaccgcgg tcaccgtcgg ggtgatcgtg
    24241 aacacgaaga agaaccaggt ggaggtccag ctgaagcggg cggtctgtgc ggaggtcggc
    24301 gcgcggattg ccatcagcag gcaggtcggc gggcgatggc ggttgatcgg catgggcgtt
    24361 ctggtcgagt gagggtgctc ctcgatacga acgccctcct gatgccggcc cagttcgggg
    24421 tcgacctgta tgacgagctt ctggccctct ttggggattt cgagccgatc gcgctcgaag
    24481 aggtggtcgg cgagctctcg gggcttgcgc ggggccgggg ccgcgatgcg gcagccgccc
    24541 gcgtgggcct tgcgctggtc cggcgttcga cggtcgtgcc gagcgggagt accgccgagg
    24601 gcgtggataa ccgggtgatc gagtatgccc ggcgggaagg gtgtacggta gtgacgaatg
    24661 accgggaact ccggaacgct ctccttcgcg agggagtcga cgtcgtctcg atgcgaaggc
    24721 agaagacgct ggagttgttg aggggataaa ctatgtatta caagatgacg ctggaggata
    24781 aggtgcgggt tccgccccac cgcctcgggg aagaccttgg tgcggtcatc ctcaatgtac
    24841 tgcaggaaca gctggaaggc agcatcgcaa aggagatcgg tatcttcatt gcggtcacga
    24901 agatcatcga tatcggcgaa ggggaactga tccccgggga cggtgccgtc tactacgacg
    24961 tgcggttcga ggcggcggtg ctccgcctgg ggctccagga ggtgatcgag ggcgaggtgg
    25021 tcgagacgac aagtttcggc gcgttcgtca gcctcgggcc gatcgatgcc atgctccacg
    25081 tgagccagat atccgatgaa tatatcaact acgacgagaa gaacggcagg ctgatctgcc
    25141 aggactcaaa gcgctacatc gccgtcggcg atgcgatccg ggcccgggtc gttgctctct
    25201 cgttgaacga acgcgagcca cgcgagagca agatcggcct caccatgcgt cagccggggc
    25261 tcgggacctc ggtctggctg gaagaggatc tcgaagagga gaagcagaaa ggagcagcgt
    25321 gatatggtcg tccgtaagaa ggtggtcaag gtctgccgcg agtgccatcg ggtcgtcgaa
    25381 ggagaggcct gcgtcatctg cggcacggcc aacctgagcg aagactgggc gggctacgtc
    25441 gtcatcatcg atcccgagca ttcggatatc gcaaagaaga tgaagatcac cctgcccggc
    25501 cggtacgcac tgaaggtccg ttgatgctca ggcttcccga aacgcaccgg gatctcttca
    25561 aaaagccgtt cggcaccctt tatggaagta tcggcgaact tcttccccgg ctttcgggcc
    25621 gggcggtcta cgccgtcggc gatgtggtga cccataacct cctcgacgcc ggggtcatcc
    25681 ctgacgtcgc catcatcgac ggctatacga tgcgcctgcc ctgcacccgc ctgcccctgt
    25741 tccgggcgag acggcggttg acggcgaaga accccgccgg gacgatcacc gatgagctcg
    25801 cgggcgcgat cgccgatctg gtccggggtc cgccgggagt gctcttcgtc gacggcgagg
    25861 aggatcttgc ggtcatcccg ctcgtccttg cagcgccgga cggtgccgcc atcctctacg
    25921 gtcaaccggg agagggcgtc gtcctccgga tcgtcgatga accggcgaaa cgggaggcca
    25981 ggttcatgct gacctgtttc gtgcgcgagt agggtgcggc cagccccata ccataattta
    26041 aataaatctg tcaacaacat tttagggata tcgatggatt tcgaaattac ccgtgacgtg
    26101 aggaacgagg tgttgagccg gagggaactt acgtttaccc tcacctttga cggcccgacc
    26161 ccctcgcgga agagcatcca ggagaagctc gccgcaatgc tgaataagaa cgagaacctt
    26221 gtcgtgctgg accttgagcg gacccgattc ggggcgatgg aactctccgg ccgcgcccgg
    26281 atctacgacg acgaggagaa gaagaagtcg accgagcggg gatatctgct caagcgcggc
    26341 gagccgaagg ccgagagcga ggcgtgaagg gcatggcggt caagagacac gagtgctacg
    26401 aactcaaggg agataaggtc gttctgcaga agcgccactg tccccggtgc ggccccggcg
    26461 tcctgatggc ggagcacaag gatcgggtgg cctgcggcag gtgcggctac accgagttcc
    26521 ggaagtagtt cctcactctc catacctata cggtcacggc gggagattcc ggccgcggcc
    26581 atctttttcg atggggcgat acctgtttta tgtctgatac gagatccgaa gacgggctgg
    26641 tgctgggact tgaggggacg gcgtggaacc tcagtgccgc cctctttgga gaagggctgg
    26701 tcgccctcca ctcctccccc tatatcccgc aaaagggcgg gatccatccg cgggaggccg
    26761 cgcagcacca cgcatcggtg atgaaggagg tcgtctcccg ggtgctcacg gagccggagc
    26821 gtatccgggc gatcgccttc tcccagggac ccggtctcgg cccttccctc cggacggtgg
    26881 cgaccgccgc gcgggccctc gcgatcgcgc ttggtgtgcc gctcgtcggc gtcaaccact
    26941 gtgttgctca catcgagatc gggagatggg cgaccgggtt tgccgacccg atcgtcctgt
    27001 atgcgagcgg cgcgaacacg caggtgctcg gcttcttgaa cggccgttac cggatattcg
    27061 gcgagacact ggatatcggg cttgggaacg cgctcgacaa gttcgcccgg agccacgacc
    27121 tcccgcaccc gggcggtccg gccatcgaga ggcttgcgcg ggagggaaac tacatcgaac
    27181 tcccgtatac ggtgaagggg atggatctcg ccttctccgg tctcgtcagc gccgcccagg
    27241 agagcacggc cccgctcgag gatgtctgcg cgggcctgca ggagaccgcg tttgccatgt
    27301 gcgtcgaggt gaccgagcgg gcgctcgcgc atgccggcaa ggacgaggtg ctgctcgtcg
    27361 gcggggtcgg ggcgaacgcg cggctgcagg agatgctccg gacgatgtgc gaggaccggg
    27421 gggcgtcgtt tgccgtcccg gagcggatct atctcggcga caacggggcg atgatcgcct
    27481 atacggggaa gatcatgctg gagcacgggg ttacgctccc catcgaggag tcgcagatcc
    27541 gccccgggta ccgggcggac gaggtggagg tcgcctggtg gaccggaccc ggcgagatct
    27601 ttgctgccgg acctctgccc gagggcggta tcgcccgggg cgcggaggcg gtcgtggaga
    27661 tccgggaagg tgatgtggtc aagcgccgga cgggcaagcg ctaccggaac ccgggcctcg
    27721 accggcggtt gattgcggag cggacccggg ccgaggcccg cctgatcgcg gcggcgcggc
    27781 gcgcgggggt tccgaccccg gtgatccggg atgtcacggc cgatacgatc gtgatggaac
    27841 gggtcgaggg cgacgtgctg aagtacgtca cgtccgccga aaacgtccgc ctcgccggca
    27901 aagcggtcgg gaggctgcac ggggcgggta tcgtccacgg cgacctgacg acgagcaaca
    27961 tgatcgtccg cggcgatcag tgcgtcctga tcgacttcgg gctcgcgtcg acgtcgccgg
    28021 aggtcgagag ccgcggagtc gacctccacg ttttattcca gacgctcgag agcaccacgg
    28081 agaactacgg ggagttgcgg gaggccttcg tcgcggggta tgcggagacg ttccccgggg
    28141 cggatgaggt cctctcccgc gagcacgagg tggaactgcg ggggcgttac ctgtgaggct
    28201 cgcggtggtg acgagcaacg cgaacaaggc gcgggaggcg gccgcgtact tcgcgggggt
    28261 gctggaggtc gagcacgtcg ccctggaatg cccggagttc cgccacgtcg atatcgggga
    28321 gatcgcccgc ggcaaggcgg agttcgccta cggggtgctc tcccggccgc tgatcgtcga
    28381 tgataccggc ctcttcatcg acgccctcgg cgggttcccg ggcccgtgcg ccgcgtacgt
    28441 ccacgacacg atcgggaacg caggagtcct gaaactcatg gagggcgtgg agaaccgcgg
    28501 cgcccgcttc gagacggcga tagcattcgc ccgcgaagac ggtatccgga tctttcgcgg
    28561 gatcctgccc gggacgatcg tcgccccccg ggggatcgga gggtttggct acgacccgat
    28621 ctttgcctct ggagagcaga cgctcgccga gatgccgctt gcggagaaga accggatatc
    28681 gcaccgggca cgagcacttg aggcgtttcg cacatggatc acgcaggagg gtggctcctg
    28741 acaaactgtt aataggattg agaacgaaca gtacccaatc gacaaatggt gttttcgaca
    28801 tggcacgatt tcccgaagct gaagcacgtc tgctcaatgt aaaggtctgt atgagatgca
    28861 acgcccggaa tgcaatccgc gcgacccgct gccgcaagtg cggctcagaa gagctccggt
    28921 ccaagtccaa ggagcgcaag gcgtaacgcc gtcgtatctg cggacttccg gccacggccg
    28981 ggccggggat ccggtatact cttttacacg ttcgccgagg agagcgaagg ttatctgccc
    29041 ttcaggcact gtaataggtg accttccggc cttcttcgct gtactcgccg cgataggtct
    29101 ctatgttccg tttgtaaccg atccgctccg taaaaccgag atcgcggagc cgttttctga
    29161 ctcgcatcac gtccgcctcg tcggcccagt cccgggtgta gacgtacatg acggcccgct
    29221 cgtcgcgcga gtccgggttc ggtttggcgg tgctgacctt tgcggatata ccgagttctc
    29281 ctctcgccgt cgcgtcacgg atcttccgcc acgcctcgtc ggcctcgccc ggagggacga
    29341 agatgagcca tttccccacc tgttcgtcgt cgaggccccg ctcaagaggg cccggcgcat
    29401 cctggacgat ccagtacatc cgggtcgtct ttgagggcag gatgccctcg cccgccctga
    29461 tcgatgcata gaccgcatcg atcccgccga accggcggat aagcgcaccg gcgagatccg
    29521 gataatcctc cccgaaccgc tcgaagaccc gggcggcatc gtctgtaaaa tcggtaccct
    29581 gttcgacgag ctcaaagagg tgcgagcccg ggtgacggag ttcacggttg aggtagacct
    29641 caaagatccc gtacgcgacc tcgcccaggg actccggatc gatctcttcc atacttactg
    29701 gtaactgccg ggaccgtaaa aagggttctg tagtgttatc ggcacggccg ggccacgcga
    29761 gactcttgct cttcttccgg gcggccacgg catcgtgccg ggtgcgccga tggtgcgggc
    29821 cacggtgagc gggagcccgg catgccgcta cgttctcctg agggcgccgg atgcgcgaaa
    29881 agccggtccg gacgtgagcg cccggggcga ttccggcgta tatcttaata gcgatccggt
    29941 ccaatagatc tctgtcatga agtggacgcg cgacttcggt ctgacgatga ggatgcttct
    30001 cacgtcgttt ctcctcctca tagtctacct gatcttcctg ggcgtcctct acgccctcgg
    30061 atttcccttt gagttcctcc tggtggttgc tgccgggatg gcgttcctcc agtatttctt
    30121 ctccgataaa ctcgtgctct ggagcaccgg cacccggatc gtcgcagagg acgagtaccc
    30181 ggagctgcac cggatgatcg aaggccttgc cgtgagggcc ggcctcccga aaccgcaggt
    30241 gggggtcatg ccttccccgg tgccgaacgc gtttgcgacc gggagaagcc cgagccatgc
    30301 cgtcgtggcg gtgaccgact cgatcatgcg gatgctcacc cgcgaggaac tcgaggcggt
    30361 gctcgcccac gagatggcgc acgtgaagaa ccgggatatg ctgacgctga cgatggcgag
    30421 tttcctctcg atgcttgcct tcctgatcat gaggaactgg ttcttcatga gcctctttgg
    30481 cggcggcaac cgcgacaaca acatgggtgc actgatcctg gtctacgtcg tctcgatcgt
    30541 cgtctggctg gtgagcacgc tcctcacccg tgcactctcc cgctaccggg agtttgccgc
    30601 ggaccggggc agcgccgaac tgacggataa cccccgtgcg ctgatatcgg cactcaagaa
    30661 gatcagcgga cggatggatt acatcccggc cgagaagaag caggaagtgg agggtgcaaa
    30721 cgccttcttc atcataccgg ccctctcggg gaacaccctg atggagctct tctcgacgca
    30781 cccgtcgctt gagaagcggg tggccgccct tgaggatctg gcggccgggg cgccgtaacg
    30841 gccttcgggg ctatataaga ctcacctttt tttgcgactc cgttgcggcg atgccggagg
    30901 ggttcgtgcc tgtatcggcg ccgcgggtcg ctgtagcggg gatcttctcg cggccagatc
    30961 ccccgggcag aaactgctgc cgggacagta tcgtacccca gaacccgggg tgattgggca
    31021 gctgatcgtc acatcagctg gcttgcaatt ttatcttacc aaaattatat aacattcttt
    31081 tgccaagaga tgcccgctct gtcagtcacg gccgaatacc cccgggaggt ctggcggctc
    31141 agcccgcagc accgcgcagg tgcagtccgg ggggcggatc cagttcccgc tgtcgtcctc
    31201 atcacggccc ggctccagtc acgagcgccc gggtcaggca gaaaaggtga ttacaatggc
    31261 tttaaaacga gtcttcggca tctttgccgt catatgcatc ctcgccctgg ccggtcccgt
    31321 tgtcgcggtc caggtgccgg aggatcagtc acaattcgtc ttcccgggcg agatccggga
    31381 agcgggcgcg gcggtagcgt tcgcaaaggg ggattacctc gcagacagat ttccagagaa
    31441 catggaggtt atagggaggt ataacgtcac tatcgatccg gcggaggtct tcaagctcca
    31501 gccgttcaac ctcgacaacc ccatagaggt gaagagagat acgccgatcg gcgcgctgga
    31561 tgcggtggcc cggacagggg cgctgaatta ctcgacatat ctctattcaa ccagaaatct
    31621 cctggtaatc gatcacatca acggccatct ctaccagggg gattggctct ggctcgtagt
    31681 ctacgagaac gggagttaca tcacatcaac ccccgatatg tgggattact ccatatccaa
    31741 cggtgagacc atctggctca tctactgcga tctcacggat ttcaactccc gctacgaatc
    31801 acgcctggac cgggcggttg caggactgtc
//
LOCUS       DAOSII010000005        30488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_1697_length_30488_cov_47.156803, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000005 DAOSII010000000
VERSION     DAOSII010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 30488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30488
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1697_length_30488_cov_47.156803"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..620)
                     /locus_tag="PLV90_00865"
     CDS             complement(210..620)
                     /locus_tag="PLV90_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95692.1"
                     /translation="MLTRSEYEAHFRDCEENLLVQQSNGIYTVTPRDGAACPHWDRGG
                     CAVYSSRPIDCRLYPYRMARIDGRDRKCTITLKMSTLCPKVDTLSRSMPETEARALVT
                     KFWREAAGDETGIAVRCESGLVDRLRSRLMAVFR"
     gene            complement(872..1162)
                     /locus_tag="PLV90_00870"
     CDS             complement(872..1162)
                     /locus_tag="PLV90_00870"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOF95693.1"
                     /translation="MRDNKPPGKAAAPDETALQVRLYALGLQACGRKVDPASLAFLQD
                     SSVVGADVPGPDTARATARSYIQGINGGEFPAKPGPFCSRCDYGAICCWRAG"
     gene            complement(1281..2819)
                     /locus_tag="PLV90_00875"
     CDS             complement(1281..2819)
                     /locus_tag="PLV90_00875"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOF95694.1"
                     /translation="MGRTPAIILTTDETMMSTYRGGMFLGFSTCAPQGILPDWLFFLA
                     FAPPVMRRDGRAVYADLGLRTIEASLIRDGFSEEEVAVVHPLDFKSMIGDETRIVAIG
                     VHDPLGINPPTSTFVDIARTGPPYNRIKFLELVKNPLLSGLTTIVGGKGAWQVSDPAV
                     MEKLHIDHIHQGMGEVSMPRVCRQILDGEEVPRIIEGEEVPAEDIPNILRPTIHGLVE
                     ISRGCGRGCAFCTPGGLKVVHKSLDHIARDIEVNVRAGSTTALLHSEDVLRYGSKGIA
                     AEPEKVLRLVDRVTAIEGVETLACSHIALATAYHHPDLVDQVSEKALSLPKAIWIGSQ
                     TGIETGSPRLMEQHMGGKCLPSKPGTWHDIVVESISHLSDAGWVLACTMVVGLPGETE
                     EDVLLTRDLVEELRGLRVFLVPMNFVAMGGSRLSLEDSFTADKMTSAHWALLGSCIEH
                     DVRLARGIKDAVYTGSLPVRLLGKYVSERLIRGGESFARSLKDGNPPRDYSVAGESYL
                     VPEI"
     assembly_gap    3018..3117
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3328..4299
                     /locus_tag="PLV90_00880"
     CDS             3328..4299
                     /locus_tag="PLV90_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95695.1"
                     /translation="MSRIAPAVILFFLAPFVAEFLLGNLPITMLSSLIVLAPMYGGGA
                     LLIREVVRRTNRGWPGMIVLALAYGVLEEGITTQSLFNPNYLNAHLLDYGFLPSLGIA
                     IPWTIFVLTIHTVWSISVPIALVEVLFSKRRTTPWLGRPGLALTCILFAIGIIATTAI
                     TLSTDAFIAPVPQLAGAVIAIIILIIIAFSLKPGEPFDTDTHPAPNARSVGVFALIAS
                     SIFMLIPHPPAWWIPVVLYVILGIVVFIVIRSWSRRRGWGDWHRLALAGGALLTYAWH
                     GFPQPSVFLGNSTVDLIGNAIFAAAAVILILIAARRLQQAGSAPLPA"
     gene            complement(4434..7448)
                     /locus_tag="PLV90_00885"
     CDS             complement(4434..7448)
                     /locus_tag="PLV90_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF95696.1"
                     /translation="MISILYVDDEPDLLELARIFIERTGEFRVATSTSALRSLADPAI
                     RSYDAIISDYQMPGMDGIAFLKAVRHQIGDMPFILFTGRGREEVVIEAINNGADFYVQ
                     KGGDPKAQFTELAHMIRQAVRGRQAKMTLAEQEQRYHDLQNANDLIQSVTPDGHFLFA
                     SKKWLETLGYTAEELPNLTVFDIIHEESIDHCMTIFQRVLSGESVGIIDAIFKTRDGK
                     KVYVEGIADGKMADGRCQYTRGLFKDITERVQAEKALCDSEHRFATIFKNSPIMLTLV
                     SAIDGTLVDVNDAFLRYTGYDREEVIGRKVEELGLFPDRPGYLRFITALRAGRPVEGM
                     RMLCRGKSGDVHTCRFFSNMTLINGRPHTLSAVEDLTEQKRAEEAARESEERYRLIME
                     NAGEGILANELTPDGPGRFIDVNDAACRILGMTREELQGMKLIDFDTLEVKRRAPALV
                     QELMQKKRVVFQAGFRTGGDREKTLDISVSLFELAGKPAILSIIRDITEVEATRSALH
                     TLVAGMVGTTGRESLDRIVESLSAWSGANCVLISEIMPDREHARVLSMLLDGKKIPEH
                     SYTLKGTPCESVAKGGFTVYPDNVAELFPESQGVRKLNIRGYAGAPLRDSAGRVVGIL
                     CIVSRTPLTLLPSAQEIIEIVASKAAAEIERMNALSSLSESEEKFRALVEHSLDGTLI
                     LDPAGTILFANHAAGRLLGVDHPDELIGGRSIMEFVVPASQNEIINDFGEVAMWVGGY
                     IAHYKILTANQGERLVEGIGKSILFNGAPSILISLRDITGRWRAENALREANRKLNLL
                     SSITRHDINNQLQILNGYAMILRDINHDPSLDGYLSRVATASSRIASMIQFTREYEEI
                     GVRLPVWQGLRTLVDEAGRGANPGGVVLENDMPEAMEVFADPLIGKVLFNLIDNALRH
                     GGKVTTVRFAATIRDEACIVTCEDDGDGIAPGLKERIFDRGFGKNTGFGLSLSREILD
                     ITGITIRETGEAGRGARFEIAVPKGQYRAGPD"
     gene            complement(8007..8447)
                     /locus_tag="PLV90_00890"
     CDS             complement(8007..8447)
                     /locus_tag="PLV90_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HOF95697.1"
                     /translation="MEWFERIVYITLIAFLVVLLTFTLLELGWLLVIGLFEDSIYRLE
                     SHELFNLFGYFLLILIGLELLETIKAYLSRREFHVEIIILVAIIAIARKVILISTATP
                     GELIGIALIIIALCSGYYLVHRAGLPRSLPPEDPPSAPESREEE"
     gene            8779..10089
                     /locus_tag="PLV90_00895"
     CDS             8779..10089
                     /locus_tag="PLV90_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOF95698.1"
                     /translation="MESIVFRTMAAIIAIVAIAAAGAVSAQPNPSLHLCVAAEENAVW
                     PGGGTGMPPDDRSAESVVTGSNRFACNLYRRLADDSESENLLFSPYSISSALAITYEG
                     ARGTTADEIRSVLHLPANDTLRREGFSRLDAGLKVAGANCTVQTANALWAEKTYPFLP
                     EYIALAERWYSANATNLDFASAPDASRQAINRWVEGRTGGKIRDLLPPASIDPMTRLV
                     ITNAISFKGTWAGPFDADRTEEAEFRVGANETVEVPMMHGDAVYPYGETATLQVLELA
                     YAHENGTGLSMLILLPKGGSLAAVEEALDPQNLSTLRRSLTRERIDVYLPKFTLDASY
                     RLPSTLAAMGMPTVFSPGADLSGMDGTKNLFISGAVHKAFVDVNEEGTEAAAATGFAG
                     NASAAPPDGGIPVFRADRPFIFLIRDDGSGSILLMGRVVRPVAA"
     gene            10248..13289
                     /locus_tag="PLV90_00900"
     CDS             10248..13289
                     /locus_tag="PLV90_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF95699.1"
                     /translation="MPEQRKKSIKAGIFTLNPHTFCITRATDGISGMLGYAPEELASF
                     ALSEIWPNKVERERFARSIGGQNAVTDISADLIRRDGELLPVTIAAASLPDTDITCIV
                     SARHPGAWEFPGGEEYLEVFDSLIDAVIILDRDGDILFANTAVATLTGVSSPAELIGQ
                     NPLQFAHPDYKEAVVRNLANLCSGHGGRLIHYPMQDLHGNERWVEALGTRIPFKGRDA
                     NLVVLRDITERKSVESELARTNQRFRDLYRLVRMMCDNVPDLIWAKDTERRYLFANRA
                     ICEKLLNAADTSEPLGRTDMFFAGRERRSRPDDPTWHTFGEICRDSDAIVMESGKAER
                     FDEYGNVRGKFLFLDVSKAPFRDDDGRMIGTVGCGRDVTEERRVEERLRWNEALLSLM
                     AQASPIAYLVVDNRTDDIHYFNSRFCDMWGLSSLEDDMRTGKIRNSDIIPPCISLIRD
                     PETFTACSPSLQDEANRLTVEDEVELVDGRCIYWFSTQIRDTDDRYLGRLYLFEDITE
                     RKNASEALRRHDAIMGAVNFAADRFLKESDWKRQMQEVLERFGRATDVSRVYIFENGT
                     DPATGNLACSQRWEWAIEGAAAGIDNPDLQSIPYEEFPRWCRKLAAGRPIVGPVWTLP
                     DNERVYFESRQVQSIAVVPIFIHQQWWGFIGFDEDRRERIWSPAEIEALQAAASIIGS
                     AILRTMNEEVYRNPVERSPMGVYLMQDGYMRYFNTRFADIFGYSRDELHGCAAQFPLV
                     APECRRDLQERYQALLSGDTESEHHEFRGMHKDGRVLYLENFATRLWFEGRPAISGSL
                     MDVTDRKQADEALRSSEERLKVLFDYAPDAFFLTDMHGILIDGNRAAERLAGCPKEEF
                     IGKSIVEAGFVPHEEYFKVEAMLNRLAAGTTAGPTEFFPVRGDGTRISIEAMAFPVTI
                     DGQSLVLISARDTSERHQLENLKKKAFLQIEENVEQLAILNDTIRNPLTVIIALAEMG
                     GTEIDRKIIKVAWEIDAIISRLDQGWLVSRKVKEFLKKHYA"
     gene            complement(13298..14200)
                     /locus_tag="PLV90_00905"
     CDS             complement(13298..14200)
                     /locus_tag="PLV90_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HOF95700.1"
                     /translation="MSTLESYLDALHRGVFQVVVPKALVTEPLEPVWKRSAINVPSPG
                     TIASYRKGQYHAHETVSEYHVHMDRYDPEKNPIMHLIDDAPLLLMIRETMETIFVTGR
                     DALQRDPLQRLADQRMSWRLRICLGAVLLAIGAILLLLVIGKTVLFFAVILPSLVLIL
                     GLLTIVEGIRGREHREHAGKKVVQGGILAGAGLVLFAFWELYLVLILLTLMVWFLSSA
                     AVTLLRAVRERRRLTKGFGYTLGLGMSSLALGVLAITLPRGLVEILLTLLAGLTLMAG
                     AFTLLDAWGLRNAARLMEAGGGFQ"
     gene            14622..16685
                     /locus_tag="PLV90_00910"
     CDS             14622..16685
                     /locus_tag="PLV90_00910"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOF95701.1"
                     /translation="MILALLIGLLFLAGGVLIALMLYCYKHRDIALARPLGLLLLCAA
                     FLPITHALNLMTETLWLKIFLLQIRFLAVPFLAVFQLIFILTYLRREEWIQGWKKVSL
                     FIIPIMTVILAQTTWFHPLYRYDYWVDLSGPLPILNFTNGIGFQVHVAYSHILLIASL
                     LLLIFVQPEIHAFYRRQRLLLLAALLLPLLCDLQYQLNLTPYPGLTLTPFALMFTGLV
                     FVGATLRQGLLEVAPVARGKVIEEMSSPMLVIDARGRMTDMNTAAVRLLGTVHDDAIG
                     SPADLVLAGHPSVLALAQGASGDNREIAIGEGLERQFFDVAVDDLRTGTGAVIGKLVL
                     LQNITERKQAEEALKESEEKYRTIIENMQDLFYRTDLDGTITMISPSGVALAGYGSPE
                     EMIGVTMQELHADPLERERFLVALRANGAVGGYPLVLKMKDGTLKHVTVNGHFFTDAE
                     GSVRGVEGIIHDVTDLHLAEQALRQANRQLNLMSSITRHDILNQLTVLLGNLSFAQEE
                     VSEGPLSGYLEKMSIAAGTIQRQIEFTRDYQDLGVRAPEWQRVSGAIRAAAAGGLPIT
                     DETDGLAIYADPMLVKVFANLMDNTIRHAESATRVHLRYRPEENGDLTLVWEDDGVGV
                     PAGEKERIFDRGFGKNTGLGLFLIREILAITGITIAERGEPGEGARFEMRVPSRAFRF
                     DSEAV"
     assembly_gap    17555..17654
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17775..17993)
                     /locus_tag="PLV90_00915"
     CDS             complement(17775..17993)
                     /locus_tag="PLV90_00915"
                     /inference="COORDINATES: protein motif:HMM:NF022829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOF95702.1"
                     /translation="MPEQISTPSYQVYTKGIDYPRSKQEVISYVREHNAPDDVIRVME
                     EMQDQQFTSASDLAVAFGEAKDRLEGRR"
     gene            complement(18200..19330)
                     /locus_tag="PLV90_00920"
     CDS             complement(18200..19330)
                     /locus_tag="PLV90_00920"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOF95703.1"
                     /translation="MVAGIWFREVEYLRGFAALAVIAVHVSMNFTRLPGAGLPALVGI
                     FVYIAAHFAVPVFVFISGWVLAARYRGGYPVPAYYRRRARTILPPYILFSALYLPIVV
                     EGAMAFAGMPAPGAMVGALLMGTAAYHLWFFVVIIQLYILYPLIAGVYDRFDRAGAAL
                     FLLLALLFVQVLWNVGAHAAGAFAGTEWYAVLIRLFPSHLLYFVLGVHVARHTDRFRS
                     MPGSLSPAWVVAAAGAGALLTGGIWVAAVYLSGGFAGATLGVFCVYRIIEPFYYVPVI
                     AALVVAARRLERGGGRPAGAFRSFGAHSFGIYLIHPLVIAAGAAAWAALTGWTWADWP
                     TYPVLFVVAAAVSYGAVRAASAVPYAGYLLGEPRALPATERS"
     gene            19417..19866
                     /locus_tag="PLV90_00925"
     CDS             19417..19866
                     /locus_tag="PLV90_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HOF95704.1"
                     /translation="MQYSEGQVGRVFTVRVDDGEDLIKEIRRFVAAMNVQSGMVHFLG
                     AVRTATLVTGPKEPVVPPTPRREEIFGGWELLGFATVHPGEDGPSLHIHATAGRGIRS
                     LTGCLRDKAEVYIVVEAIITEFVGITSRRLPDEKTGVDLPVFDEFLP"
     gene            19863..21662
                     /locus_tag="PLV90_00930"
     CDS             19863..21662
                     /locus_tag="PLV90_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOF95705.1"
                     /translation="MSGQPEFLPMTGEEADRLGIDRFDVILISGDAYVDHPSFGTAIV
                     GRVLWNAGYSVGVIAQPDWRSDADLRRLGEPRLFFSISAGNVDSLVNAFTPNLKRRSS
                     DVYSPGGRLLRPDRATLVYTDRVHALFPETPIVLGGIEASLRRFAHYDYWSDSVRQSI
                     LADAPADLLVFGMGERQVVTIADRLAAGERTLTDIPGTACRVDRKTWRGMDPTGYVVL
                     PDYAEVKSDRYAYARAFALHYNEQDPFRGRPAAQPHPKTVVIQNPPAIPLSGPELDRV
                     YELPYTRRAHPSYAEPVPALEPVRFSVVSHRGCFGACSFCALTHHQGRIIQSRSADSI
                     VREVERMAKMPEFTGIVQDIGGPTANMYGIHCGRWETAGTCPDRRCIDCPALDRSHAD
                     QLGLLRRLLDIPGVRRVFIASGIRYDLIGPEDEEYLSRICADHVSGHLKVAPEHVSAR
                     VTACMGKPPRAVFDAFRERFEVLQAGKKKRQYLVPYLISGHPGCRMEDMVELAEYVRD
                     TGLYTEQVQDFTPTPMSISTTIYHTGLDPFTLEEVYVPKGREKQIQRALMHYRDPANY
                     ALVREGLRATGREDLIGSAWKCLVPARGKRRSP"
     gene            complement(21666..21944)
                     /locus_tag="PLV90_00935"
     CDS             complement(21666..21944)
                     /locus_tag="PLV90_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95706.1"
                     /translation="MTCRRHAAQSDEWRSTVMRRADEIAGRLALPPSPLPGGWRSGTM
                     QMLDAAVTPGTVQNIAASLAVAMFVIPRIPCRPLAIAAGFAAGSIFGK"
     gene            complement(21968..22246)
                     /locus_tag="PLV90_00940"
     CDS             complement(21968..22246)
                     /locus_tag="PLV90_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOF95707.1"
                     /translation="MRGGEEAAHPAEAITAGAHLTDEARSAALERQCHRCAGCNAPLT
                     AGSTHFDIRPPVICGGSPLPGNLEALCPFCHQNFMRRVRVRLAGHRNK"
     gene            complement(22252..22515)
                     /locus_tag="PLV90_00945"
     CDS             complement(22252..22515)
                     /locus_tag="PLV90_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95708.1"
                     /translation="MGRVTDLQVDDLVGIKDDTDAHAGHGGGAALVGSESRTRTRGVV
                     LVGPAQALGTTLVRALDPRAAVEGSGRPKGHRKIYPPGGHLPA"
     gene            complement(22931..23236)
                     /locus_tag="PLV90_00950"
     CDS             complement(22931..23236)
                     /locus_tag="PLV90_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95709.1"
                     /translation="MRPIVLVLLLIAVAGVAGTEAINVSLPDRDAPEAVLGTVESMWG
                     FVEWLLQVADDLLSLIRNVLGSLGVGGEEVERLMGAMDSGKDLVNRTLEEGAPSGGG"
     gene            23441..24133
                     /locus_tag="PLV90_00955"
     CDS             23441..24133
                     /locus_tag="PLV90_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HOF95710.1"
                     /translation="MQYEITGDNLQMVTLRLASGESACAEAGAMVNMSGNMQMTTHMK
                     GGLFKGLKRMVTGEGLFMTEFTPQGGGGFVSFAGNIPGKIFALNLDGGEFIAQKDAFL
                     CSEKGIDLDIAFTKRLRSGFFGGEGFILQRLSGRGNVFLHCCGDIMEMTLAPGEVVRV
                     ETGLVVGFESTVDYNIALAGGVKTVLFGGEGLFLATLTGPGRVILQSMDIAKLASALM
                     PYLPVQHNSSGR"
     gene            24166..25188
                     /locus_tag="PLV90_00960"
     CDS             24166..25188
                     /locus_tag="PLV90_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2117 domain-containing protein"
                     /protein_id="HOF95711.1"
                     /translation="MNLVMVVHGPEAFDAGDVERMIGLLAPRLVLVAGVMARTAAEES
                     GLPVTCTDERPSVVFRALVEPSCLVNRGKTPESGRIFGELVAGRLSGLIHVECSDGTV
                     YCWDSADENLAAEIASRTGYVLVRAGSDRRSRPEGVREIRGCIPGEAVFVNGLVVGTA
                     TAETVVLESAGGTLRVVSGLEPKPHGFEKLLRAGLPDIGIAWCKSGPVRTASPRQGGR
                     ARSGGRIAVVDHCGHTLYRDIGEDVCGVLAVGDDTTAVCGHICSHAGIPVFGVIDGDG
                     DAIVKPGYAPGSVVVEVTHGRDDDIGREVIATRNLWAASWEEWVEETLRALEGRVRIV
                     VDRRGE"
     gene            25192..26334
                     /locus_tag="PLV90_00965"
     CDS             25192..26334
                     /locus_tag="PLV90_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95712.1"
                     /translation="MQCAECKGRGFCGLERCPIMSRFYARLPVRPADHYEGAAPSVFV
                     GSYGYPKVAGGPLMIDDADHPPDWVRQGLGIDDIVGLRARTIRGAGEAKGLGRGIQEI
                     ALSSTPLDVEVRFVKPISFDLRFDGTIAPVGLTGAIRDMDVLGNARVDRAVDRATSDT
                     DLGATDACEILNASGIDVYRITQLLTAGLLGRKRRIVPTRWAITAVDDTVSTRLKKKI
                     ARYPPLSGIEVFSAALYGNHIVCLLVPGDWKYEMIEVWGKQSLWGGGSETIACDGEGA
                     TKSGYSPLAGAYYSARLAVAEYLESVQRSARVLVLRNVTGEYWAPLGTWVVREAARNA
                     MRGPKTSCASLPGAVDLASRLIGFTHWRPHSRLIPELATQKTLFDF"
     gene            complement(26331..27977)
                     /locus_tag="PLV90_00970"
     CDS             complement(26331..27977)
                     /locus_tag="PLV90_00970"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HOF95713.1"
                     /translation="MQFLEFARICERLEGISGRLDMIEEVAGVLPRLGDDELPVFVRF
                     IMGRVFPDWSTKKLGVGPNLLYDAVAYVIGRKRETVREAINATGDAGLAVEDLLARKE
                     QTSFFIQELDLVDVYRELGRMAAAGGQRSQKEKLRVAQGLFGNARPLEGRYLTRLMLE
                     ELRIGMGEGNVRDAVARAFALDVRLVEHAHQAMNDLGEVALLARRDPDALSRVTIEPF
                     RPVKMMLAQAGTIAGQVADHGEVAVEYKYDGSRFQFHKVGGVCRIYSRRLEEVTESLP
                     DIARQLMEATDRDVILDGEAVAVRDGRPMPFQFVIRRFRRKHEVNAMMEKIELVPRVF
                     DILYIDGESLLDRSLAERRTILEGALRAHVAEQFRVSDTAAAEAIYADALARGHEGVM
                     VKVPASPYTPGVRGRQWVKVKPGVETLDLAVVGAEWGEGRRAKMFGSFLLAVQDQGRL
                     LPVGKVATGITDETLADLYALFKDSVIAHSGKEVTLEPAVVFEVGYSEIQTSPNYASG
                     YALRFPRFVRVREDKSVDEVETLDSLAGRYGGQKNGQGSI"
     gene            28065..28298
                     /locus_tag="PLV90_00975"
     CDS             28065..28298
                     /locus_tag="PLV90_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95714.1"
                     /translation="MNPDDEIKEMLRDLIWLNALIATELVQITENTSQILRKAAPPEA
                     CIAEHAALRATALDIADRYRPGTILRQHIEKHQ"
     gene            complement(28332..>30488)
                     /locus_tag="PLV90_00980"
     CDS             complement(28332..>30488)
                     /locus_tag="PLV90_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HOF95715.1"
                     /translation="DGGLLFAGNLTYVTNGTQADTDAWLVKIDPSGAEAWNRTYGGPD
                     VNASANAVIPAGDGGFLFVGSTATLEGSESDAWAVRVNETGGEVWRRVLGGAGNDTMN
                     AAARLSDGDFVVAGSTESSGAGMADVWVIRLDRSGGEVWNRTFGSPDDDAGQAVITTP
                     DGNLLIAGTFTERPDNATVDTDALLIKLTPAGDTLWNWVYGEPGVDESAAAVTRTADG
                     GYLFAGETGYPGTDDTDAWLVKTDADGAVVWSRTFGGENPGDRALSVLEIAEGEYLFA
                     GAFNATEKGGVANTDAWAVRLGPAPAPAPTPTVAPIKPPKAPVVHQKPPVAVSPAPPP
                     APTPTIKPTEKPTKPTEKPTVKPTEKPTKKPIEKPPEKPIERPTLKPPKKPIGKPTIP
                     LWPKPTHTVKPTERPGDDDDRNALSGTVWYDLNADGSRTSGEPGIPGISVRLIGQRTM
                     VDAAVTGPDGSYRFATIPAGDYTGVEFLLPDGYACTLPGQGSDARPMATDVAFAEALD
                     GRQTLDAGLVGDYRSATPATAYGWILGTVWSDDDQNGIKDETSGLTDVEVRLIDAGGD
                     PVAKTRTGYHDRYTSLYLFGPLLPGEYSLAFSPPDGYVFTAAGGDSHADPSDGTTPLF
                     TLAGSETVVRDAGLIPAAAGKTTGGVGGGSDNATAGESPAMTLAPENKTAGDSSAVTP
                     GPDNATAGDGGLTPPAEEPPSPEGGDRTASGSPSTD"
BASE COUNT         5524 a         9796 c         9491 g         5477 t
ORIGIN      
        1 caatcatgag ggactctcac tccctcgact ggggttcgaa tccccatagc agcattctgt
       61 ttttcgggtt caaacctgac agcgagcggt tttatgcctc ccctgcacgt gaacgttcaa
      121 cagtaccaac ggccggaggg atagaggagg gagtacaagg cggatgcctg ccggtaatgg
      181 gcacggcagg gagagatctc ctgacccctt caccggaaca ccgccataag ccgggaccgc
      241 agccgatcga cgagccctga ttcgcaccgg acggcgatgc ccgtctcgtc tccggcagcc
      301 tctctccaga acttcgtgac aagtgcgcgt gcttcggtct ccggcatcga cctgctcagc
      361 gtatcaacct tcgggcagag cgtgctcatc ttgagggtaa ttgtacattt cctgtctctc
      421 ccatcgatcc gcgccatcct gtagggatag agccggcagt cgatcggtcg gctcgaatag
      481 acggcgcaac caccacgatc ccagtgcgga caggctgcac cgtctcttgg cgttacggtg
      541 tagatgccgt tggactgttg tacgagaagg ttttcctcgc agtctctaaa gtgcgcctcg
      601 tactcgctcc tcgtcaacat aagaccggtc aatcgcgtac aacagtgttg atccgtgctg
      661 catgtgtcgc acagattatc catcacgctt cccatcccct gacctcctga cacctgcccg
      721 gtgtcgggga tcccttatgt atgtatggtg ctgctaacgt ggggcgtcct tgagaccctg
      781 ctcctcgaat gaaagatcct tcgtctgggc acgcggaggt ccatgacgcc cctgaagaag
      841 cccggtggag cgggcggcgt acctcccgcc cctaccccgc cctccagcag catatcgccc
      901 catagtcgca ccgcgagcag aatggccccg gctttgccgg gaactccccg ccgttgatcc
      961 cctggatgta actccgggcc gtcgctctcg cggtatcagg cccgggcaca tcggcgccga
     1021 ccaccgatga gtcctgcagg aacgcaagcg acgccgggtc caccttcctg ccgcaggcct
     1081 ggaggccgag cgcgtagagc cggacctgca gtgcggtttc gtccggcgcg gcggccttac
     1141 cgggtggctt gttgtcccgc accttgagcc cgtcgcgtag ccgcccatgc ggcgcatatc
     1201 ccggagcccg gcgtctcctc gcacccgaca caggtaaaac agcggcagga ccctcccgtc
     1261 ctcccggaga gccggaaaaa ttagatctcg ggaaccaggt aactctcccc ggcaaccgaa
     1321 tagtcgcggg gcgggttgcc gtccttcagg ctccgggcaa acgattcgcc cccccggatg
     1381 agcctctccg agacgtactt gccgaggagc cgcaccggca agctgccggt gtagacggca
     1441 tccttgatgc cccgggccag acgcacatcg tgctcgatgc aggaaccgag gagtgcccag
     1501 tgtgcagagg tcatcttatc cgccgtaaaa gaatcctcca gcgagaggcg ggagcccccc
     1561 atggcaacga agttcatggg gaccaggaat acacggagac ctctgagttc ctccaccagg
     1621 tcccgggtca gcaggacatc ctcttccgtc tctcccggca gaccgaccac catggtgcag
     1681 gcgagcaccc agccggcgtc gctgaggtga gagatgctct ccacgacgat atcgtgccac
     1741 gtcccgggtt ttgacggcaa gcattttccc cccatatgct gctccatcag gcgggggctc
     1801 cccgtctcga tgccggtctg agagccgatc cagatcgcct tcggcaacga aagggccttc
     1861 tccgagacct gatccaccag gtcggggtgg tgataggcgg tggcaagcgc gatgtggctg
     1921 caggcaagcg tctccactcc ctcgatagcc gtcacccggt ccacgagccg gagcactttc
     1981 tccggttccg ccgcgatccc cttcgaaccg tatcgcagca cgtcctcgga gtgaaggagc
     2041 gctgtggtgc ttccggcccg cacgttcacc tcgatgtcgc gggcgatatg atcgagggat
     2101 ttatgcacca ccttcagacc tcccggcgtg cagaaggcgc agcccctccc gcatccccgc
     2161 gagatctcca ccaggccgtg gatggtcggc cgcaggatgt tcggtatatc ctcggccgga
     2221 acctcttctc cctcgatgat gcggggaacc tcctcgccat ccaggatctg ccggcagacg
     2281 cgaggcatcg agacctcccc catcccctgg tggatgtggt cgatatgcag tttctccatg
     2341 accgcagggt ccgagacctg ccaggcgcct ttgcccccga cgatggtcgt caggccggag
     2401 aggaggggat ttttcacgag ctccaggaac ttgatgcggt tgtatggcgg acctgtccgg
     2461 gcgatatcca cgaatgtcga tgtcggcggg ttgatcccca ggggatcgtg caccccgatc
     2521 gccacgatcc gtgtctcatc gccgatcata ctcttgaagt ccaggggatg gaccacggca
     2581 acctcttctt ctgaaaaacc gtcgcgaatg agcgacgctt cgatagtccg cagccccagg
     2641 tccgcataga ccgctctccc gtctcttcgc atcaccggcg gggcaaacgc aaggaaaaaa
     2701 agccagtcag gcagaatgcc ctggggggcg caggtagaga agccgaggaa catcccgccc
     2761 cggtaggtgc tcatcatcgt ctcgtcggtc gtgagaataa ttgccggagt tcgtcccatt
     2821 aaaaccaccc tctggccgga gtgaaccaaa atcacattta tatttatctt gaaatatgtc
     2881 gcactccgca tgaatccggt ccatcgcccg gagcggagaa agtgccgggt atcccttcct
     2941 gtcacagaaa aaacgccgga gatggacgca ccccgatgcc cgggtgctga ggtgccggtc
     3001 atcgctctgc cgggcagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagg
     3121 tcacatcatt cgaggccggg cgccagggta gcagagcacc ggggcccgtg gcgcccggag
     3181 accgggccat gatgtcggca acagctcact acggtatgag gatccggtgc cggaaggtct
     3241 taagtcgatg caggttatcg ggctatcatg cccggcagag gatcggcgag cactgctcgt
     3301 agaacacaac actccatcgg agtgttcttg agccgtatag ctcccgcagt catactcttc
     3361 ttccttgctc cgttcgttgc cgaattcctg ctcggcaacc tccccatcac catgctctcc
     3421 tcccttatcg ttctggcgcc tatgtacggc ggcggcgcac tcctgatcag ggaggttgtc
     3481 cggcggacca acaggggttg gccgggcatg atcgtgcttg ctcttgccta cggcgtcctg
     3541 gaggagggca tcaccaccca gtcgctcttc aacccgaact acctcaacgc gcaccttctc
     3601 gattacggct ttctgccgtc gctcgggatc gccatcccgt ggacgatatt cgtcctcacc
     3661 atccacaccg tctggagcat cagcgtgccg atagcactcg tcgaggttct gttctcgaaa
     3721 cgccggacca ccccctggct cggccgaccg ggacttgcac tcacctgcat cctctttgcg
     3781 ataggcatca tcgccacaac ggcgatcacc ctgagcaccg atgccttcat agcccctgtg
     3841 ccccagctgg caggcgccgt gatagccatc atcatcctca tcatcatcgc attcagcctg
     3901 aaaccgggcg agcctttcga taccgatacg cacccggcgc cgaatgcccg gtcggtcggt
     3961 gtgttcgcac tgatagccag ctcgatattc atgttgatac cgcatcctcc tgcctggtgg
     4021 ataccggtgg tcctctacgt catcctgggt atcgtcgtgt tcatcgttat ccggtcgtgg
     4081 tcccggcgcc gggggtgggg cgattggcac cggctggcgc tcgccggtgg ggcgctcctc
     4141 acctatgcct ggcacgggtt ccctcaaccc tccgtcttcc tgggcaattc caccgtcgat
     4201 ctcatcggca acgccatatt cgccgcagcg gcagtcatcc ttatccttat cgcagcacgg
     4261 aggctgcagc aggcgggtag cgcccccctt cccgcataac cgccgggtat atggttgccc
     4321 gggtcgccgg cgctatctcc acccggcgag gggcaaccac catctcctca ctttccggtg
     4381 tcccggaact ttacgaagca ccgtggccgc tccggagggc gttgtcgccg gccttaatcc
     4441 ggcccggccc ggtactgccc cttcggcaca gcgatctcga accgtgcgcc cctgcccgcc
     4501 tccccggtct cccggatcgt gatgccggtg atatcaagga tctcccgcga cagagagagt
     4561 ccaaaaccgg tgttcttccc gaacccccgg tcaaagatcc gctccttgag acccggcgcg
     4621 atgccgtcgc catcatcctc acaggtaacg atgcatgcct catcgcgaat agtggccgcg
     4681 aaccggacgg tggtgacctt cccgccatgg cgcagggcgt tgtcgatcag gttgaacaag
     4741 accttgccga tgagcgggtc ggcaaagacc tccatcgctt cgggcatatc gttctccagc
     4801 acgaccccgc cggggttggc acccctgcct gcctcgtcga ccagagtccg gagaccctgc
     4861 cagacgggga ggcgcactcc gatctcctcg tactctctgg tgaactggat catcgacgca
     4921 atccggctgc ttgccgtggc gacgcgggag aggtagccgt caagcgaagg atcgtgattg
     4981 atatcgcgca atatcatggc atacccgttc agaatctgga gctggttgtt gatgtcgtgc
     5041 cgggtgatgc tcgagaggag gttgagtttt ctgtttgctt ccctgagtgc gttttcggcc
     5101 ctccagcgcc cggtaatatc ccgcagagat atgaggatcg acggggcgcc gttgaagaga
     5161 atggacttcc cgataccctc gaccaaccgc tccccctggt ttgccgtgag gatcttatag
     5221 tgtgcgatat acccgccgac ccacatggcc acctcgccga aatcgttgat gatctcgttt
     5281 tgtgatgcag ggacaacgaa ctccattata ctcctccctc cgatcagttc gtcgggatga
     5341 tctaccccta atagccggcc cgcggcatgg tttgcaaaga ggatggtccc cgccggatcg
     5401 aggatgagcg tgccgtcaag ggagtgctcc acaagagccc gaaacttctc ctcgctctcc
     5461 gaaagcgacg aaagagcgtt catgcgctcg atctcggctg ccgcttttga agcaacgatc
     5521 tctatgatct cctgcgcgga gggcagcagg gtgagcgggg tccgactcac gatacagagg
     5581 atgcccacga cccgtcccgc ggagtcccgg agcggggctc cggcatagcc ccggatgttg
     5641 agcttccgga cgccctggct ctcaggaaag agttcggcaa cgttgtccgg gtagacggtg
     5701 aacccgccct ttgcgacact ctcgcagggc gtccccttca gggtatagga gtgctccggg
     5761 atcttcttac catcaaggag catggagagc acccgggcat gctcacggtc cggcatgatc
     5821 tcgctgatca ggacgcagtt cgccccggac caggcgctga ggctctccac tatccggtcg
     5881 agcgactccc tgccggtcgt ccccaccatc ccggcgacga gcgtatggag cgccgatcgg
     5941 gtggcctcga cctccgtgat gtcgcggatg atcgagagaa tcgccggctt tcccgcaagc
     6001 tcgaagagac tgacactgat atcgagggtc ttctcccggt ccccccctgt ccggaagccg
     6061 gcctggaaga cgacccgctt cttctgcatc aactcctgta caagcgcagg ggcgcgtctc
     6121 ttcacctcca gcgtatcgaa atcgatgagt ttcatgccct gcagttcttc gcgggtcatc
     6181 ccaaggatcc ggcaggcggc atcgttcacg tcgatgaatc ttcccggccc atccggcgtg
     6241 agttcgtttg ccagtatccc ctcgcccgcg ttctccatga tcaggcgata gcgctcttcg
     6301 ctctcgcggg ccgcctcctc cgcccgcttc tgttccgtta gatcctcgac cgccgagagg
     6361 gtgtggggcc tgccgttgat cagggtcatg ttcgagaaga accggcaggt atggacgtcc
     6421 ccgctcttgc cgcggcacag catcctcatg ccctcgacgg ggcgccccgc ccggagggcc
     6481 gtgatgaacc ggagatatcc cggccgatcc gggaagagcc ctaactcctc tactttcctg
     6541 ccgatcacct cttcccggtc atatccggtg tagcgtaaaa acgcgtcgtt gacgtcgacg
     6601 agcgtgccgt cgatagccga gacaagggtg agcatgatgg gactgttctt gaagatcgta
     6661 gcgaacctgt gttctgagtc gcagagcgcc ttctccgcct gcacacgttc ggtgatgtcc
     6721 ttgaagagtc cccgggtgta ctggcatctc ccatccgcca tcttgccgtc cgcgatgccc
     6781 tcgacgtaga ctttcttccc gtcccgggtt ttgaagatcg catcgatgat ccccacactc
     6841 tcgccggaga gcacccgctg gaagatcgtc atgcagtggt cgatgctctc ctcgtgaatg
     6901 atatcgaata ccgtaaggtt cggcagctcc tccgccgtat acccgagcgt ctccagccat
     6961 ttcttgctgg caaagaggaa atgtccgtca ggggtaacgc tctggatcag gtcgtttgcg
     7021 ttctggaggt catggtagcg ctgctcctgt tcggcaaggg tcatcttcgc ctgcctcccc
     7081 ctcaccgcct gccggatcat gtgggcgagc tcggtgaact gggccttcgg gtccccgcct
     7141 ttctggacgt agaagtcggc gccgttgttg atcgcttcga tgacgacttc ctcgcggcca
     7201 cggccggtga agaggatgaa gggcatatcc ccgatctggt gccggaccgc ttttaagaac
     7261 gcgatcccgt ccatgcccgg catctggtag tcggatatga tggcatcgta cgacctgatt
     7321 gccggatctg caagagatct caacgccgac gtcgatgttg cgacccgaaa ctctcctgtt
     7381 cgttcgataa agatccgggc cagctcgagg agatcgggtt cgtcatcaac atagagaatc
     7441 gatatcacat caccacttct cctgcagatc gtggtcggcg cgcagggcag gcatgcctcc
     7501 cgtgagggaa agaatgccgg cacctgtagc ccgccagggc gatcgatact acctctctca
     7561 ccacgtcgcc ttataagcgt tgcagtctgt cgatacccga taacgccgga tatcggggta
     7621 tatccgcagg cgacctatct cgcaccccgg ttattcacgg gagagctgct aacgtgatat
     7681 attgaaataa tagcctgttt gccggcggat ctctcttctc ccggcgggtt ttgcaagatg
     7741 catcgataca tccgggtggt ccggccatcg agatcggcgg gggcgcgatc cggcatgcgc
     7801 tccccgggcc ccgccggtgg gttcatggcg gagaacgccc gcgatggaca ccaccctgct
     7861 cttgtggcgg gtgtggccgt cgacgacccc acgacaggag ctggccctga gcggagagag
     7921 agcagatccc ctgtcggtgc cgggcaaggg ccgccggcga gagcggtcgg ccgctcccgg
     7981 ttgagcagga agagcagtcg ccgtggtcac tcttcttccc ggctttccgg cgcggatgga
     8041 gggtcctccg gggggagaga gcgcggcaac cccgcccggt gtacaaggta atacccgctg
     8101 cagagggcga tgatgatcag cgcgatgccg atgagctcgc ccggggttgc cgtgctgatg
     8161 aggataacct ttcttgcgat ggcgatgatt gcaacgagga tgatgatctc gacgtggaac
     8221 tccctccggc tcaggtaggc ctttatcgtc tccaggagct caaggccgat gaggatcagc
     8281 aagaagtagc caaagaggtt gaacaactca tggctctcaa gccggtagat agaatcctca
     8341 aacaggccga tgaccagtaa ccatcccagt tcgaggagcg taaaggtgag gagcactacc
     8401 aggaatgcga tgagcgtaat gtagacgata cgctcgaacc actccacgaa acggcgcata
     8461 tatggtttat cgcctcgatt tgcataaata tctgccgaaa actatatgtg cactcagacc
     8521 ctgcgacggg cggaatcccg ggggcggtgc agtcattact atatactctc tcctccatca
     8581 cgccgcagcg tgagaacccc gaccccgtat gtcctctcac ccttaccacc tgcaggtttc
     8641 gcgtataatc tgcaggatat gcgcatacac ccggtgctgc cggtacccgg gagcaacggc
     8701 agggggactt gcacgggggc gcggccgcac gcaccgggcc cccgaaggtc cggcaacagc
     8761 cagatgaggg tgaaccatat ggaatctatc gtattcagga cgatggctgc catcatcgcc
     8821 atcgtcgcca ttgctgcagc gggagctgtt tcggcgcaac cgaacccgtc gctgcatcta
     8881 tgtgtcgccg ctgaagagaa cgctgtctgg ccggggggtg gaaccggtat gcctcccgac
     8941 gaccggtcgg ctgaaagcgt cgttacgggc agcaaccgtt tcgcttgcaa cctctatcgg
     9001 cgcctcgcag acgattcaga gtcggaaaac ctccttttct cgccttacag catatcgtcg
     9061 gcccttgcga tcacctatga gggcgcccgc ggcacgaccg ccgatgagat ccggtcggtt
     9121 ctccatctcc cggcaaacga cacccttcgg cgggaggggt tttcccggct cgatgccggc
     9181 ctgaaggtcg cgggggctaa ctgcaccgtt cagacggcaa acgccctctg ggcggagaag
     9241 acgtatcctt tcctcccgga gtacatcgct ctcgcggagc gctggtactc tgcgaacgcg
     9301 acgaaccttg actttgcgag cgcccccgat gcgtcgcggc aggcgatcaa ccggtgggtc
     9361 gaagggcgaa ccggggggaa gattcgcgac ctcctgcctc ccgcctcgat cgatcccatg
     9421 acccggctcg tcatcacgaa cgcgatctcc ttcaaaggaa cctgggccgg cccgttcgat
     9481 gcggacagga cagaagaggc ggagttccgg gtgggcgcga acgagaccgt ggaggtcccg
     9541 atgatgcatg gagacgccgt ctacccgtat ggagagaccg cgaccctcca ggtgctcgaa
     9601 cttgcctatg cgcacgagaa cggaacaggg ctctcgatgc tgatcctgct cccgaagggc
     9661 ggaagccttg cggctgttga ggaggctctc gatccgcaga acctctcaac gctgcgccgc
     9721 tcgctcaccc gtgagcgcat cgacgtttat ctcccgaagt tcacgctcga tgcgagctac
     9781 cgccttcctt cgacgcttgc ggcgatgggg atgccgacag tgttctcccc cggtgccgac
     9841 ctctcgggga tggacggcac gaagaacctc ttcatcagcg gcgccgtcca taaggcgttc
     9901 gtggatgtga acgaggaggg caccgaggcc gcggcggcga cagggttcgc cgggaacgcg
     9961 agtgctgcgc ccccggacgg gggaattcct gtattcaggg cggatcgtcc gttcatcttt
    10021 ctcatacggg atgacgggag cgggagcatc ctcctcatgg ggcgggttgt acggccggtg
    10081 gcggcctgac cctcccgggc ggtcaggtca tctttaccgg tacggcgggc gattcttgag
    10141 ccgaggctgc gagaacgcgc cctggttgtc cggcccgaaa ggaaatagat accagtataa
    10201 ggcgagagat agagatctct tgaaagacga tggaaaagga aacccggatg cccgaacaga
    10261 gaaaaaaaag cataaaagcg ggtattttca ccctcaaccc gcacacgttc tgcatcaccc
    10321 gggccaccga tggtatatcc ggcatgctcg gctacgcgcc ggaggaactc gcatccttcg
    10381 ccctctccga aatatggccg aataaggtag agcgggaacg gttcgcccgg tcgatcggcg
    10441 ggcaaaacgc cgtcacagac atctccgccg acctgatccg ccgtgacggt gagctcctgc
    10501 ccgtgacgat tgccgcagcc tccctcccgg atacggatat cacctgcatc gtctccgccc
    10561 gccaccccgg tgcatgggag tttccaggag gggaggagta cctggaggtc tttgactccc
    10621 tcatagacgc cgtcatcatc ctcgaccggg acggcgacat cctgtttgcc aataccgcgg
    10681 tcgcaacgct caccggcgtc tcttcgccgg ccgagctcat cggccaaaac cctctgcagt
    10741 tcgcccaccc cgactacaag gaggccgtcg tccggaacct cgcgaacctg tgttccgggc
    10801 atggcggccg cctcatccac tacccgatgc aggatctcca cgggaacgag agatgggtcg
    10861 aagcgctcgg cacgaggatc ccctttaaag ggcgggacgc aaacctcgtc gtgctccgcg
    10921 atataacgga gcgaaagagc gtggagagcg aactcgcccg gaccaaccag cggttccgcg
    10981 acctctaccg cctcgtccgg atgatgtgcg acaacgtccc cgatctcata tgggcaaaag
    11041 atacggagag gcgatacctc tttgcgaaca gggccatctg cgagaaactc ctcaacgctg
    11101 ccgataccag cgaaccgctg ggcaggacgg acatgttttt tgccgggcgc gagcgacgat
    11161 cccgcccgga cgaccccacc tggcacacct tcggagagat ctgccgggac tccgatgcga
    11221 tcgtcatgga gagcggaaag gcggagcggt tcgacgagta cgggaacgtc agggggaagt
    11281 ttctcttcct cgacgtcagc aaggccccgt tccgggacga cgatgggcgg atgatcggga
    11341 ccgtggggtg cggccgcgac gtgaccgagg agcggcgggt ggaagagcgc ctccggtgga
    11401 atgaagcgct gctctccctg atggcccagg cctccccgat cgcctatctg gtcgttgata
    11461 accggacgga cgatattcat tacttcaata gccggttctg cgatatgtgg gggctctcaa
    11521 gcctggaaga cgatatgagg acgggaaaga tccggaacag cgatatcatc cctccctgta
    11581 tatcgctcat cagggatcct gagacattta ccgcatgctc tccgtcactc caggatgagg
    11641 cgaaccggtt gaccgtcgag gatgaggtcg agctcgtcga cgggcgatgc atctactggt
    11701 tctccaccca gatccgcgac acggacgacc gttacctggg aagactctac ctctttgagg
    11761 atatcaccga gcggaagaac gcatccgagg ctctccgccg gcatgacgcc atcatgggcg
    11821 cagtgaactt tgcggcggac aggttcctca aggagtcgga ctggaagaga cagatgcagg
    11881 aagttctcga gcggttcggc agggccaccg acgtcagcag ggtgtacata ttcgagaacg
    11941 gcacggatcc cgcgaccggc aacctcgcct gcagccagcg ctgggagtgg gctatagagg
    12001 gggccgccgc agggatcgat aacccggacc tccagagtat cccctacgaa gagtttcccc
    12061 gctggtgcag gaaactcgcg gccgggagac ctatcgtcgg gccggtatgg acgctccccg
    12121 ataacgagcg ggtttacttc gaatcccggc aagtccaatc catcgcggtc gtcccgatct
    12181 tcatccacca gcagtggtgg ggcttcatcg ggtttgatga ggaccggagg gagcgtatat
    12241 ggtcgccggc ggagatcgag gcgctccagg cagcggcaag catcatcggt tcggcaatcc
    12301 tccggacgat gaacgaggag gtctaccgga acccggtgga gcgctccccg atgggcgtct
    12361 acctgatgca ggacggctac atgcgctact tcaatacccg gtttgccgat atattcgggt
    12421 attcgcgcga cgagctgcac ggttgcgccg cacagttccc cctggtcgcc ccggagtgcc
    12481 ggagagatct gcaggagcgg taccaggccc tcctctccgg cgataccgag tcggagcacc
    12541 acgagttccg cggcatgcat aaagacgggc gggtcctcta cctcgagaac tttgcaaccc
    12601 gcctctggtt cgaggggcga ccggccatca gcggcagcct gatggatgtg accgaccgga
    12661 aacaggcgga tgaggcgttg aggagttcgg aggagcggtt gaaagtcctc ttcgattatg
    12721 ccccggatgc cttcttcctc accgatatgc atgggatcct tatcgacggc aaccgggcgg
    12781 cggagaggct ggcaggctgc ccgaaggaag agttcatcgg caagagcatc gtcgaagccg
    12841 gattcgtgcc gcatgaagaa tacttcaagg ttgaagccat gctcaaccgg cttgcggccg
    12901 ggaccaccgc cggcccgacc gagttcttcc ctgtccgtgg cgacggcacc cgcatctcca
    12961 tagaggcgat ggcgttcccg gtgacgatcg acggccagtc cctggtcctc atcagcgctc
    13021 gcgacacttc agagcggcat cagctggaaa acctcaagaa aaaggcgttc ctccagatcg
    13081 aggagaatgt cgagcagctt gccatcttaa acgacaccat tcgcaatccc ctcaccgtca
    13141 tcatcgctct tgcggagatg gggggcacgg agatcgatcg aaagatcatc aaggtggcct
    13201 gggagatcga tgcgatcatc agccggctcg accagggatg gctggtctca aggaaagtga
    13261 aggagttttt aaagaagcat tacgcgtgag aggatggtca ttggaagccc ccgcccgctt
    13321 ccatcaggcg ggcggcgttc cggagccccc acgcatcgag aagcgtgaac gcgccggcca
    13381 tgagcgtgag cccggcaagc agggtaagaa ggatctcgac cagtccccgg ggcagggtga
    13441 ttgcgaggac cccgagcgca agcgaactca tcccgaggcc gagggtatac ccaaaaccct
    13501 tcgtcagcct tcggcgctcc cggaccgccc gcagcagcgt gacggcggcg ctcgagagga
    13561 accagaccat gagggtcagc aggatgagga cgaggtagag ttcccagaag gcgaatagaa
    13621 caaggcctgc gcctgccagg ataccaccct ggacgacctt tttgcccgca tgctctctgt
    13681 gctccctccc ccggatgccc tcgacgatcg tgagaagacc gaggatgagc acgagggagg
    13741 gcaggatcac ggcaaagaag agtacggtct tgccgatcac caggagaagg aggatagcgc
    13801 ctatcgcgag gaggaccgca ccgagacata tccggagtct ccagctcatg cgctggtcgg
    13861 cgaggcgctg cagggggtct ctttgcagcg catctctgcc cgtcacgaag atggtctcca
    13921 tcgtctcgcg gatcatgagg agaagggggg cgtcgtcgat caggtgcatg atggggttct
    13981 tctcggggtc gtaccggtcc atgtgaacat ggtactccga gacggtctcg tgggcatggt
    14041 actggccctt ccggtaactc gcgatggtcc ccgggctcgg cacattgatg gcggaccgtt
    14101 tccagaccgg ttcgagcggt tcggtcacca gggcctttgg tacgacgacc tggaagaccc
    14161 cccggtgcag tgcatcaaga tacgactcaa gcgtgctcaa cccatctcct cctcagccgt
    14221 tctcaggatt cgccgacacc cgcgggaagg tctcttgaga aacgaggcct gtagatgcgc
    14281 atatcacact ttctggagga ggttgtatta aatgtgaggg tccggtacgc ttcgtcgccg
    14341 tggagttact cgtagaagag ggggcaaccc cgccggtcag gcagattccc atccgggaaa
    14401 agacagcggc aggacacgta ttctccagcc gggtgtcccg ggagcgagag cgccccggct
    14461 tgcacctatc aacgcgaatt cctcaaactc ttataattag gtttatttaa ttgaatgggc
    14521 tcctaaaccc gctcttttcc ccggatctgc gctttcatcg cctgttccta aacgcaacat
    14581 taaacatccg tcaatcaaaa cactacccct agagactgtc catgatactc gccctcctga
    14641 tcgggctcct cttccttgcc gggggggtgc tcatcgccct catgctctat tgctacaagc
    14701 accgcgacat agcactggca cggcccctcg gactcctgct gttgtgtgcc gccttcctgc
    14761 ccatcaccca tgcactcaac ctgatgacgg agacgctctg gctcaagatc ttcctgctgc
    14821 agatccggtt cctggccgtg ccgttcctcg ccgtctttca actcatattc atcctcacct
    14881 acctgagaag ggaggagtgg atacagggct ggaaaaaagt ctccctcttc atcatcccga
    14941 taatgaccgt catcctcgcc cagaccacct ggtttcaccc gctctaccgg tacgattact
    15001 gggtcgacct ctcgggaccg ttgcccatac tcaacttcac caacggaata ggattccagg
    15061 tccatgttgc atactctcac atcctcctga tcgcgagtct cctcctcctc atattcgtcc
    15121 agccggagat ccatgccttc taccggcgcc agcgcctcct tctcctggcc gcgcttctcc
    15181 tccccctcct ctgcgatctc cagtaccagc tgaacctcac cccgtacccg ggactgacgt
    15241 tgacgccgtt cgccctgatg ttcaccggcc tcgtctttgt gggagcaacg ctcagacagg
    15301 gcctcctcga ggtagccccg gtcgcccggg ggaaggtgat cgaggagatg agctcgccga
    15361 tgctggtgat cgatgcgcgg gggcggatga ccgatatgaa caccgcagcg gttcggctcc
    15421 tcgggacggt gcatgacgat gccatcggca gcccggcgga tctcgtcctc gcgggccacc
    15481 cctcggtcct tgccctggca cagggcgcat ccggggataa ccgggagatc gccatcggag
    15541 agggactgga gaggcagttc ttcgatgtag ccgttgacga tctgcggacc gggaccgggg
    15601 cggtcatcgg aaaactggtc cttctccaga atatcaccga gcgcaagcag gcagaagagg
    15661 ccctcaaaga gagcgaagaa aaataccgga ccatcatcga gaacatgcag gatctgttct
    15721 accgcaccga tctcgatggg accatcacca tgatcagtcc gtccggggtg gcactggcag
    15781 gctacggttc gcccgaagag atgatcggag tgaccatgca ggaactccat gccgatcctc
    15841 tggaacgtga gaggttcctc gtcgcgctca gggcgaacgg cgcggtcggg ggatatccac
    15901 tggtgctcaa gatgaaagac ggaactctga agcacgtgac ggtgaacggc cattttttca
    15961 ccgatgcaga aggttcggtc cggggcgtgg aagggatcat ccacgacgtc accgatctcc
    16021 acctggctga acaggcgctc cggcaggcga accggcagct caacctgatg agcagcataa
    16081 cccggcacga tatcctcaac cagctgacgg tcctcctcgg caacctctcg ttcgcacagg
    16141 aggaagtgtc cgaaggaccc ctctcaggat atcttgagaa gatgagcatc gccgccggga
    16201 ccatccagcg ccagatcgag ttcacccgtg actaccagga cctcggcgtc cgtgctccgg
    16261 aatggcaacg cgtatcgggc gcgatccggg ctgcggcggc cgggggactt ccgataaccg
    16321 atgagacgga tggccttgca atctacgcgg atccgatgct cgtgaaggtc tttgccaacc
    16381 tgatggacaa cacgatccgg cacgccgagt cggcgacccg ggtccatctc cggtatcgtc
    16441 cggaggagaa cggggacctc accctcgtct gggaggacga cggtgtcggc gttcctgcag
    16501 gggagaagga acggatcttc gaccgtggat tcgggaagaa cacggggctc gggctcttcc
    16561 tgatccggga gatcctcgcg atcaccggga tcaccatcgc cgagagaggg gaacctggag
    16621 agggggcacg gttcgagatg cgggtgccct cccgggcgtt ccggttcgat tcggaagcgg
    16681 tgtgacgtcg ccgtcgcccg gtgcgcctgt cgcgccggag agacgcccct cgcgacctca
    16741 cccggggtag gggcctcccg atccgccccg ccggatacat ccggatctct ccctgccggg
    16801 agatcaacta tgacagagaa agagcggatg ctatccggga aggcagacat cgcccaggac
    16861 gaagaaccga agcgggataa cctgagagcc aaaagaccga ctcgattatt caacgggacg
    16921 aggtcgctcc tggtgagatg gtctccgggg gtaggggctg tcgggtaaga aagcggttga
    16981 aggtgtcatg agtaaccaca tgacccccgc gagctatgca ttgcatatcc ggtgtgcaga
    17041 agggcgttgc agataagcat ggcaatattg ttgtgcaaat tcttaattga gcccgacgat
    17101 cacgtcatcc tgccacaagg caaaacggaa gatggcatgc gaccacggga gggatgcgga
    17161 tgcatgttcg acagaggcat gagtctgtta tccgtcggga ggtgtgaaga agcacggaag
    17221 gtgggtcaat caaacgttac tgcggccgtt gaggaggcat cgcgcaaccg gtgcgcaacg
    17281 ttcgacggtg ctaccacaac agattactgg aaacgtgttg cgagagagag gacgggtgcc
    17341 atattttctg atgccggact atgagcagag cggtttcacg gcatcccttc atccggcctg
    17401 cccggcaaga tctctgttcc acgccccttt gcggcagcgc gggagatctt ctggttatcc
    17461 ccgcccggac gcctggaact ccccttcgca ccttcgcggc ttcgcgcggg aggccgtatc
    17521 gtttatgcac ctgagctcgc gcgttgccgc gaagnnnnnn nnnnnnnnnn nnnnnnnnnn
    17581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17641 nnnnnnnnnn nnnnagctcg cgcgttgccg cgaagtcccg tccggtcgcc ggcaggccgc
    17701 gagattgccg cgatcgatgg ttccggggcc tgtaaaagag cgggtgtgct ccacccggga
    17761 aagggtgcgg agtgttaccg ccgcccttcg agccggtctt ttgcctcgcc gaacgctact
    17821 gcgagatctg acgccgatgt gaactgctgg tcctgcatct cctccatgac ccggatcacg
    17881 tcgtcggggg cgttgtgttc acggacgtag gatatgacct cctgtttgga tctggggtag
    17941 tcgatgcctt tcgtatacac ctgatacgag ggtgtactta tctgctctgg catattctct
    18001 tcaccatccg cagccggggc tgcggccccc gataaacctg agatcactta atggttgggt
    18061 ccgggtgccg tggcaccggg tgctctccac gggccggagc cgcgagattc ttcgttggcc
    18121 ggggcccgca ccgccagaat ggtgaggcaa tccgggtctt cgcacacgtg gacttgagtg
    18181 tgccctcctc tcctgcggtt cacgaccgct ccgttgcggg caacgctcgc ggctccccga
    18241 gcaggtaccc ggcatacggg accgctgacg ccgcccgcac cgccccgtaa ctcaccgccg
    18301 ccgccaccac gaagagcacc gggtaggtcg gccagtccgc ccacgtccat cccgtaaggg
    18361 cggcccatgc cgccgcgccg gccgcgataa cgagggggtg gatgaggtag atcccgaacg
    18421 aatgcgctcc aaacgaccgg aaagcccccg cagggcggcc gccgcccctc tccagccgcc
    18481 gggctgcaac gacgagagcg gctatgaccg ggacgtagta gaacggttcg atgatccggt
    18541 agacacagaa gacgccgagc gttgcgccgg caaacccgcc cgagaggtag acggccgcga
    18601 cccagatccc tccggtgagg agagcccccg cacccgcggc ggcgacgacc cacgccgggg
    18661 agagcgatcc gggcatcgac cggaaccgat ctgtatgtcg ggcgacgtgg acgccgagaa
    18721 cgaagtagag gaggtgcgac ggaaagaggc ggatcagcac cgcataccat tccgtgcctg
    18781 caaacgcccc ggcggcatgg gcgcccacgt tccagagtac ctggacgaag aggagggcga
    18841 ggaggaggaa gagcgccgcc ccggcccggt cgaagcggtc atagaccccg gcgatcaggg
    18901 ggtagaggat gtagagctgg atgatcacga cgaagaacca gaggtggtag gcagcggtcc
    18961 ccatgaggag ggcgcccacc attgccccgg gagcgggcat gccggcaaac gccattgccc
    19021 cctccacgac tatcgggagg tagagggcgg agaagaggat atatgggggg aggatcgtcc
    19081 gggcacggcg ccggtagtag gccgggacgg ggtaaccacc ccggtatcgg gccgcgagca
    19141 cccagcccga tatgaagaca aagaccggca ccgcaaagtg ggcggcaatg tagacgaata
    19201 taccgacgag ggcgggcagc cccgcccccg ggagccgggt gaagttcatg gagacgtgga
    19261 ccgcgatcac ggcaagagct gcaaaccccc gcaggtattc gacctcccgg aaccagatcc
    19321 ccgcgaccat atctcgcatt gcgaggccgg cgcttatgaa tccctcgccc gggcactata
    19381 ttatcatgcc gcatcaccta cctggagagt gagtccatgc agtactcgga agggcaggtc
    19441 ggccgggtct ttacggtcag ggtcgacgat ggagaggatc tcatcaagga gatccggcgg
    19501 ttcgtggcgg cgatgaacgt ccagagcggt atggtccact ttctcggcgc cgtccggacc
    19561 gcgacgctgg tgacgggacc gaaagaaccg gtcgtgccgc cgacaccgcg gcgcgaggag
    19621 atattcgggg gctgggaact cctcgggttc gcgacggtcc atcccgggga ggatggaccg
    19681 tcgctccaca tccacgccac ggcggggagg gggatacggt cgctcaccgg atgcctccgc
    19741 gataaggcgg aagtttacat cgtcgtcgaa gcgatcatca cggagttcgt cgggatcacc
    19801 tcgcggaggc tcccggacga gaagaccggc gtcgacctgc ctgtctttga cgagttcctc
    19861 ccatgagcgg gcagccggag ttcctcccga tgacggggga ggaggcagat cggctcggca
    19921 tcgaccggtt cgacgtcatc ctcatatccg gcgacgccta cgtcgaccac ccctccttcg
    19981 ggaccgctat cgtcggccgg gtcctctgga acgccggcta ctcggtcggc gtcatcgccc
    20041 agcccgactg gcgcagtgac gccgacctcc ggcgcctggg cgagccccgc ctcttcttct
    20101 cgatatcggc cgggaacgtc gactccctgg tgaacgcctt cacgccgaac ctgaaacgcc
    20161 ggagttcgga cgtctactcg cccggcggaa ggcttctgcg gcccgaccgg gcgacactcg
    20221 tctacaccga ccgggtccat gccctcttcc cggagacgcc gatcgtcctg ggcgggatcg
    20281 aggcgagcct ccggcggttc gcccactacg actactggtc tgactcggtc cgccagtcca
    20341 tcctcgccga cgcgccggcc gatctcctcg tcttcggcat gggcgagcgc caggtggtca
    20401 cgatagcgga ccgcctcgcc gccggtgaaa gaaccctcac ggatatcccc ggcaccgcct
    20461 gccgggtcga caggaagacg tggcggggca tggacccgac cgggtacgtc gtcctcccgg
    20521 actacgcgga ggtcaaatcc gatcgctacg cctatgcacg ggcgtttgcc ctgcactaca
    20581 acgagcagga tcccttccgg ggccggccgg cggcgcagcc gcacccaaag accgtcgtca
    20641 tccagaaccc gccggcgata ccgctatcgg ggcctgaact cgaccgggtc tacgaactcc
    20701 cctacacgcg gagggcgcac ccttcctacg ccgaacccgt accggccctc gaacccgtcc
    20761 ggttctcggt cgtcagccac cgcggatgct tcggcgcctg ttcgttctgc gccctcaccc
    20821 accaccaggg aaggatcatc cagagccgga gcgcggactc gatcgtccgc gaggtggagc
    20881 ggatggcaaa gatgccggag tttacgggca tcgtccagga tatcggcggg ccgacggcga
    20941 acatgtacgg cattcactgc ggcagatggg agaccgccgg cacctgtccc gaccgccgct
    21001 gtatcgactg ccctgcgctc gaccggagcc acgccgacca gctcggcctg ctccgccgcc
    21061 tcctggatat accgggggtg agacgggtct tcatcgcatc cggcatccgc tacgacctga
    21121 tcggcccgga ggatgaagag tatctctccc gcatctgcgc cgaccacgtc tccgggcacc
    21181 tcaaggtcgc ccccgaacac gtctcggcac gggtcacggc ctgcatgggt aaaccccccc
    21241 gcgcggtctt cgatgccttc cgcgaacggt tcgaggttct ccaggcaggg aagaagaaac
    21301 ggcagtacct cgtcccctac ctcatatccg gccacccggg ctgccggatg gaggatatgg
    21361 tcgaactcgc ggagtacgtc cgggacaccg gcctttacac cgaacaggtc caggacttca
    21421 cgccgacgcc gatgagcata tcgacgacga tctaccacac cgggcttgac cccttcaccc
    21481 tcgaggaggt ctacgtcccg aaaggccggg agaaacagat ccagcgggcg ctcatgcact
    21541 accgggaccc ggcaaactac gccctcgtcc gcgaagggct ccgtgcgacc gggcgggagg
    21601 acctgatcgg gagcgcgtgg aagtgtctcg tcccggcccg ggggaagaga cggagcccct
    21661 gaggttcact tcccgaagat cgagcccgcg gcaaacccgg cggcgatcgc caggggccgg
    21721 cacggtatgc gggggatcac gaacatcgcc accgcgaggg aggccgcgat gttttgcacc
    21781 gtccccgggg tgacggcggc atcgagcatc tgcatcgtgc cggaacgcca gcccccggga
    21841 aggggcgatg gaggcagcgc gagcctcccc gcgatctcat ccgccctcct catcaccgtc
    21901 gatcgccact cgtcgctctg cgctgcatgc cgtctgcagg tcatgatcga cggtatgacc
    21961 ccgggtacta cttattccta tggccggcaa ggcgcacccg gacccggcgc atgaagttct
    22021 ggtggcagaa ggggcagagg gcctcgaggt tcccgggcag gggggacccg ccgcagatca
    22081 ccggcggcct gatatcaaag tgcgtggaac cggcggtgag cggtgcgttg cacccggcac
    22141 accggtggca ctgccgctcg agggcggccg accgggcctc atccgtgagg tgcgctcccg
    22201 cggtgatcgc ctctgcagga tgcgccgctt cctctcctcc gcgcatgatg atcacgcggg
    22261 gagatgcccc cctggcggat aaatcttgcg atggcctttt ggtctcccgg agccttcaac
    22321 ggcagcccgg ggatccagag ccctcacgag ggtcgtgccg agcgcctgag caggaccgac
    22381 gagcacgacc ccacgggtcc tggttctcga ctcgctcccc acgagcgctg cgccgccgcc
    22441 atggccggcg tgggcgtcgg tatcatcctt gatcccgacg aggtcatcga cctggagatc
    22501 tgtgaccctg cccatgaagg aggaccctgg aagcgtcatc cggggcaaca gcgtcctcac
    22561 gacggacgag atccgggtct cttcgttcgc cgtagaactg ctcccccgcc ccttgacggg
    22621 ttctcctcca cccccctccc catgcccgga tgggcatgag atctctctgg cccacccatt
    22681 ttttgagacg accggggaag aaaacagcag cgcaacagac cggatcctac ttacactcgt
    22741 gtgagaggat gcggaggttg ccggaaccgg cgcatgcggt tctggtgaca ggcagggggc
    22801 ctcgagtctc cccggcgtct gcgattcgcc ggagaccgtc tcctcgcgga ggtcaaagtg
    22861 cgtcgacccg gcggtcaggg ggacgtttga cccggcgcac cggcgagaag gataggaagc
    22921 gtctgcccga ttaacccccc ccggatggcg caccctcttc gagggtcctg ttcacgagat
    22981 cctttcccga gtccatcgcc cccataaggc gttcgacctc ctcgcctccc accccgagag
    23041 acccgagcac attacggatg agcgagagca ggtcgtcggc gacctgcagc agccattcga
    23101 caaaccccca catcgactcg accgtcccga gcacggcctc cggcgcgtcg cggtcgggca
    23161 gcgagacgtt gatcgcctcc gttcccgcga cccctgcgac ggcaatgaga agcagaacca
    23221 gaacgatcgg tctcatactg ccaccctccc ggccgggacg gtaccggagc gtatgcccgg
    23281 ggcagggggg atgctaaacg atccattctc catacaaatc gttacggcat tgatcccggt
    23341 taaagccacc gctccggagg gggatttcgg aaacagagcc gccggcccgg agagaacaga
    23401 gagaaaagac ctattgcccg gggatgaaag aagttccggt atgcaatacg agatcaccgg
    23461 agataacctc cagatggtga ccctccgcct cgcctccggc gagagcgcct gcgccgaggc
    23521 gggcgccatg gtcaacatga gcgggaacat gcagatgacg acccatatga agggcggtct
    23581 cttcaagggg ttgaagcgga tggtgaccgg cgaaggcctc ttcatgaccg agttcacccc
    23641 gcagggcggc ggggggttcg tctcgtttgc cgggaacatc ccgggaaaaa tatttgcgct
    23701 caaccttgac ggaggtgagt tcatcgccca gaaagacgcc ttcctctgct ccgaaaaggg
    23761 catcgacctc gatatcgcgt tcacgaagcg cctccggtcg ggtttcttcg gcggcgaggg
    23821 attcatactc cagcgcctct ccggccgggg aaacgtcttc ctccactgct gcggcgacat
    23881 catggagatg acccttgcgc ccggcgaggt ggtcagagta gaaacagggc tcgttgtcgg
    23941 gttcgagagc accgtcgact acaacatcgc tcttgccggc ggcgtgaaga ccgtcctctt
    24001 cggcggggaa gggctcttcc tggccacgct gaccgggccg ggccgcgtca tcctccagtc
    24061 gatggacatc gccaaactcg caagcgccct gatgccctac cttcccgttc agcacaactc
    24121 gtcgggacgg taacctgata gtccgcgccg ccccaggagt acgggatgaa cctcgtcatg
    24181 gttgtgcacg gtcccgaggc cttcgacgcc ggggacgtgg agcggatgat cggtcttctt
    24241 gccccccggc tggtgctcgt cgcgggggtt atggcccgga ccgccgccga ggagtcgggg
    24301 ctcccggtga cctgcacgga cgagcggccg agcgtggtct ttcgcgccct cgtcgaacct
    24361 tcatgcctgg tcaaccgggg gaagacccct gaatccggca ggatatttgg cgagctcgtc
    24421 gccggccgcc tctctgggct catccacgtc gagtgctcag acggcaccgt ctactgctgg
    24481 gactctgccg acgagaacct cgcggcagag atcgcaagcc gaaccggcta cgtcctcgtt
    24541 cgtgccggga gtgatcgccg ctcccggccg gagggcgtgc gggagatccg cggctgcata
    24601 ccgggggagg cggtcttcgt gaacgggctt gtcgtcggga ccgcgacggc ggagacggtc
    24661 gtcctcgaaa gcgcgggcgg gaccctccgg gtcgtctccg gccttgagcc aaaaccccac
    24721 gggtttgaga aactcctccg ggcgggcctc ccggatatcg gcatcgcctg gtgcaagagc
    24781 gggccggtca ggaccgcttc gccccggcag ggcggccgtg cccggagcgg ggggaggatt
    24841 gcggtcgtcg accactgcgg ccacaccctc taccgcgata tcggggagga cgtctgcggg
    24901 gtgcttgccg tcggcgacga caccaccgcc gtctgcggcc acatatgctc ccacgccggc
    24961 ataccggtct ttggtgtgat cgacggcgac ggcgatgcca tcgtgaagcc cgggtatgcc
    25021 ccggggtcgg tcgtggtcga ggtcacgcac ggccgcgacg acgatatcgg ccgggaggtc
    25081 atcgcgacgc ggaacctgtg ggccgcctcc tgggaggagt gggtggagga gacgcttcgc
    25141 gctctcgagg gaagggtccg gatcgtcgtc gatcgccgtg gagagtgaag catgcagtgc
    25201 gccgagtgca aggggcgagg gttctgcggt cttgagcgct gcccgatcat gagccggttc
    25261 tacgcccgcc tcccggtccg gccggccgac cactacgagg gggctgcgcc gtcggtcttt
    25321 gtggggagtt acggctaccc gaaggtcgcc ggggggccgc tgatgatcga cgacgccgac
    25381 cacccgccgg actgggtccg gcagggcctc gggatcgacg acatcgtcgg gctccgggcc
    25441 cggacgatcc gcggcgccgg ggaggcgaag gggctcggcc ggggcatcca ggagatagcg
    25501 ctctcgagca ccccgctcga cgtggaggtg cggttcgtca aacccatatc gttcgacctc
    25561 cggttcgacg gaacgatcgc cccggtcggg ctcaccggcg ccatccggga tatggatgtc
    25621 ctcgggaacg cccgggtgga ccgggcggtc gaccgggcga cctccgatac ggatctcggc
    25681 gcgaccgacg cctgcgagat cctcaatgcc tccggcatcg acgtctaccg gatcacccag
    25741 ctcctcaccg ccggcctcct cgggcggaag cggcgcatcg tccccacgcg gtgggctatc
    25801 acggcggtcg acgatacggt ctcgacccgc ctcaagaaga agattgcgcg gtatcccccg
    25861 ctctccggca tcgaggtctt ctcggccgcg ctctatggga accatatcgt ctgcctcctc
    25921 gtccccggcg actggaagta cgagatgatc gaggtctggg ggaaacagtc gctctggggc
    25981 ggcgggagcg agacgatcgc ctgcgacggc gagggcgcga cgaagtccgg ctactcgccg
    26041 ctcgcggggg cttactactc ggcccgcctt gcggtcgcgg agtacctgga gagcgtgcag
    26101 cgttcggcca gggtgctcgt tctcagaaac gtcaccggcg agtactgggc gccgctcggc
    26161 acctgggtcg tccgggaggc ggcgcgaaac gccatgcggg gtccgaagac gtcgtgcgcg
    26221 agcctccccg gggcggtcga cctcgcctcc cggctgatag ggttcaccca ctggcgcccc
    26281 cacagcaggc tcataccgga acttgcgacg cagaagacgc tctttgactt ctagatcgag
    26341 ccctgcccgt tcttctgccc gccgtagcgc ccggcgaggg agtcgagcgt ctcgacctcg
    26401 tcgacgctct tgtcctcgcg gaccctgacg aaccgcggga accggagcgc gtagccgctt
    26461 gcgtagttcg ggctcgtctg gatctcggag taacccacct cgaagaccac cgccggctca
    26521 agcgtcacct cttttcccga gtgggcgatc acggagtctt tgaagagcgc gtagaggtcg
    26581 gcgagcgtct cgtcggtgat cccggtcgcc accttcccga cggggaggag ccggccctgg
    26641 tcctggaccg cgagcaggaa cgagccgaac atcttcgccc gccggccttc gccccactcg
    26701 gccccgacga ccgcgagatc gagggtctcg acgccgggtt tcaccttcac ccactggcgg
    26761 ccccggacac cgggggtgta gggcgaggcg gggaccttca ccatgacgcc ctcgtggccg
    26821 cgggcaagag catcggcgta gattgcttcc gccgcggcgg tgtcggagac ccggaactgc
    26881 tctgcgacgt gggctctgag cgccccctcg aggatcgtcc gccgctcggc aagcgatcga
    26941 tccagcaggg actcgccgtc gatatacagt atatcaaaga cccgggggac gagctcgatc
    27001 ttctccatca tcgcgttcac ctcgtgcttg cgccggaacc ggcggatgac gaactggaac
    27061 ggcatgggcc tcccgtcccg gacggcgacc gcctccccgt ccaggatcac gtcgcggtcg
    27121 gtcgcctcca tgagttgccg ggcgatgtcg gggaggctct ccgtgacctc ctcgagacgg
    27181 cgggagtaga tccggcagac gccgcccacc ttgtggaact ggaacctgct cccgtcgtac
    27241 ttgtactcga ccgcgacctc gccgtggtcg gcaacctgcc cggcgatggt gcctgcctgg
    27301 gcaagcatca tcttcaccgg ccggaacggt tcgatcgtca cgcgggagag cgcatccggg
    27361 tcccgccggg caaggagggc gacctcgcca aggtcgttca tcgcctggtg ggcatgctcg
    27421 accaggcgga cgtcgagggc aaacgcccgc gcgacggcgt cgcggacgtt cccctcgccc
    27481 atccctatcc ggagctcttc gagcatcagc cgcgtgaggt accgcccttc aagcgggcgg
    27541 gcgttcccga agaggccctg cgcgacacgg agcttctcct tctgcgaccg ctgccctccc
    27601 gccgccgcca tccgcccgag ttcgcggtag acgtcgacga ggtcgagttc ctgtatgaag
    27661 aacgacgtct gctcttttct cgcgaggaga tcctcgacgg caaggccggc gtcgccggtg
    27721 gcgttgatcg cctcgcggac ggtctcccgc tttctcccga tgacgtaggc caccgcgtcg
    27781 tagaggaggt tggggcccac gccgagtttt ttggtgctcc agtcgggaaa gaccctgccc
    27841 atgatgaacc ggacgaagac ggggagttcg tcgtcgccga gtcgggggag gacgccggcg
    27901 acctcctcga tcatatccag gcgccccgaa atcccctcga ggcgctcgca gatgcgggcg
    27961 aactccagaa attgcatacc catctcctga agtatttccc ggcggggtga atcaaccctt
    28021 ctcttctggg cggtttgata ggggagcgcc gccaaccttc atccatgaac cctgacgacg
    28081 agatcaagga gatgctccgc gacctcatct ggctcaacgc cctgatagcg accgaacttg
    28141 tccagatcac cgagaacacg tcgcagatcc tcaggaaggc ggctccgccg gaggcctgca
    28201 tcgccgagca cgcggccctg cgggcgacgg cgctcgatat agccgaccgc tacaggcccg
    28261 ggacgatcct ccggcagcac atcgagaagc accagtgagg atgtcgggcc ggcggtgcag
    28321 gagcccggat atcagtcagt tgacggggaa ccggaggcag tccggtcccc tccttccggg
    28381 gaagggggct cctccgccgg cggggtaagc ccgccgtcgc cggcggtcgc gttatccggg
    28441 cccggtgtca cggccgaaga atcgccggcg gtcttgttct ccggggcgag cgtcatggcc
    28501 ggggattcgc cggcggtcgc gttatccgag cctcccccca caccgcccgt ggttttcccg
    28561 gcggccgccg ggatcagccc tgcatcccgg acgaccgtct cgctcccggc gagggtgaag
    28621 aggggcgttg tgccgtccga tgggtcggca tggctatccc cgccggcggc cgtgaacacg
    28681 tagccgtccg gcgggctgaa cgcaagcgag tactccccgg gcaggagggg accgaagagg
    28741 tacagggagg tgtagcggtc gtggtagccc gtacgggttt tggccaccgg atcgccgccg
    28801 gcatcgatga ggcggacctc gacgtcggtc aggccggatg tctcatcctt gatgccgttc
    28861 tggtcatcgt cgctccagac cgtcccgagg atccatccat aggccgtcgc cggtgtcgcg
    28921 gaccggtaat ccccgacgag gcccgcatcc agggtctgcc ggccatccag agcctccgcg
    28981 aacgctacgt ctgtcgccat cgggcgggcg tcgctgccct ggcccgggag ggtgcaggcg
    29041 tagccgtccg gcaggaggaa ctcgacgccg gtgtagtcgc cggcggggat ggttgcgaac
    29101 cggtatgaac cgtcgggtcc ggtgaccgca gcatcaacca tggtccgttg gcctatcaac
    29161 cgcacgctga tgcccgggat gcccggctcg ccggatgtgc ggctgccgtc cgcgttgagg
    29221 tcgtaccaga ccgtgcccga cagcgcgttc ctgtcatcgt cgtcgcccgg cctctccgtc
    29281 ggtttcacgg tatgggtcgg tttcggccag aggggtatcg tcggttttcc aatgggtttt
    29341 ttaggaggtt tcaatgtcgg tctctcgatg ggtttctcag gaggtttctc tatcggcttt
    29401 ttggtcggtt tctccgtcgg ttttacggtc ggtttctcgg tgggtttggt cggtttctcc
    29461 gtcggtttta tggtcggggt gggcgccggg ggtggggccg gactgaccgc caccggcggt
    29521 ttctggtgca ccaccggcgc cttcgggggc tttatgggag ccactgtcgg cgtcggtgcg
    29581 ggcgccgggg caggcccgag cctcaccgcc caggcgtccg tattcgccac cccgcccttc
    29641 tctgtggcgt tgaaggcccc tgcaaagagg tactcgccct ctgcgatctc caggaccgag
    29701 agcgcacggt cgccgggatt ctcaccgcca aacgtcctgc tccagaccac cgcgccgtcg
    29761 gcgtcggtct tcacgagcca ggcatcggta tcgtcggtgc cggggtagcc cgtctccccg
    29821 gcgaagaggt agccgccgtc cgccgtcctg gtgacggccg cggccgactc gtccacgccg
    29881 ggttcaccgt agacccagtt ccacagggta tccccggcgg gcgtgagctt gatgaggagc
    29941 gcatcggtgt cgaccgtcgc gttgtcgggc cgctccgtga acgtgccggc gatgaggagg
    30001 ttgccgtccg gggtggtgat gaccgcctgg ccggcatcgt catcggggct cccgaacgtc
    30061 cggttccaga cctcgccgcc cgaccggtcg aggcggatca cccagacgtc ggccatcccc
    30121 gcccccgacg actccgtgct gccggcgacg acgaaatcac catcggagag ccgggcggcg
    30181 gcgttcatcg tatcgttccc ggccccgccg aggacccgcc tccagacctc gccgcccgtc
    30241 tcgttcaccc tgacagccca ggcgtccgac tcgctcccct cgagggtcgc ggtggagccg
    30301 acgaagagga aaccgccgtc gccggccggg ataacggcat ttgccgaggc gtttacgtcc
    30361 ggcccgccgt aggtccggtt ccaggcctcg gcgcccgaag gatcgatctt cacgagccag
    30421 gcgtcggtgt ctgcctgcgt cccgttcgtg acgtaggtga ggttgccggc gaagaggagg
    30481 ccgccgtc
//
LOCUS       DAOSII010000006        29923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_1752_length_29923_cov_50.585376, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000006 DAOSII010000000
VERSION     DAOSII010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 29923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29923
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1752_length_29923_cov_50.585376"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..365)
                     /locus_tag="PLV90_00985"
     CDS             complement(18..365)
                     /locus_tag="PLV90_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95716.1"
                     /translation="MARKFDERDFAILRKLAPEYSGILCPESGHEFHSILPPVSNHIA
                     ADEEDFAERVGRLSDEDWIYLTDRILSGREDLGCMPEEDIETILIRIEKAVSDEAAER
                     IRRLYRLSGCGVL"
     gene            complement(420..605)
                     /locus_tag="PLV90_00990"
     CDS             complement(420..605)
                     /locus_tag="PLV90_00990"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HOF95717.1"
                     /translation="MPVVTIRMAKGRTLEQKRRLAAEITAAITETLGVDPERVTLFFE
                     ELDTENIARAGRLLAES"
     gene            complement(686..1618)
                     /locus_tag="PLV90_00995"
     CDS             complement(686..1618)
                     /locus_tag="PLV90_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOF95718.1"
                     /translation="MMPFYEFLEEIRPAVNRRIGEVTGDEGSIDPGVVPLLLRGKRMR
                     AGLLLHTHAALTGRKAPTRRALDLACAVELAHAASLILDDMLDGDILRRGLPSHHLTR
                     GTGRAVLDAVGVLALPYALAAPCGAEYVAMLASAQQSMARGAAWEMLGGSGLSSIELY
                     DAIIARKTGCLFALAAAWGAMAADEDEDVVAAFADFGLFTGKAMQIADDIADLHAPAA
                     GVPGRRPGSEVLLLQGIAGEDRTDQALAARLDTEIARAADRLGDGARQRLTPGEREAF
                     GQAVRDIVGLTIAGGLPPGTATFQVVRPGALVYR"
     gene            1791..2702
                     /locus_tag="PLV90_01000"
     CDS             1791..2702
                     /locus_tag="PLV90_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOF95719.1"
                     /translation="MGRLEEGDAFRDWLAGILADRLTGWRGKIRVYRIEPASHVVCRY
                     EFAGGPSVVGKFFGAPTGARTRYNTDAAMKNEFLRLRRVSGWIRVPHAIATNNRFHSV
                     LVTEHVPGPTVRELLGAGVSLYDPLTGVAHLLRRLHDSTRTSCKRERDFAYYHAILDQ
                     NNLSGSRRKRFDRLLGAWWHSARLGPEGCMVHGDATLTNYLFNGDTCAIDFEGSRNHT
                     HPIRDLGILAAELRTSGASISTAEDYIGHLLWHYSRGEEEFRRYTAVLPFFMALGYLR
                     IARLPWRAAERDWLLEEAEACLCAIHR"
     gene            2678..3340
                     /locus_tag="PLV90_01005"
     CDS             2678..3340
                     /locus_tag="PLV90_01005"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOF95720.1"
                     /translation="MSLCDPPVAGVLFDCYGTLIDILTDERDTETYRNLARWLIYQGV
                     MVAPDDLRVLYTCHVREAIEQIGEPYPEVRVEEIFAGICAEHAIRKIDAGRLGVETAR
                     AFRAASLRRLGIIEESRRLLDLFCAEKLGVVSNGQRVFSECEMRAFGLYDRFDFVIFS
                     SDLGYQKPDTRIYAAALGRMRLSAPEVLFIGDNPENDVAAPRRFGMRALHVEEAWRRY
                     SD"
     gene            3435..4049
                     /locus_tag="PLV90_01010"
     CDS             3435..4049
                     /locus_tag="PLV90_01010"
                     /inference="COORDINATES: protein motif:HMM:NF018933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOF95721.1"
                     /translation="MPFIRGSPPPGDWIGVDLNATGSIAVVAHPASGGVVMLGEQPEG
                     AGTPERCSGGKRKALRAARDRESSARGDLNRRIAREIVKMARNLECGIKLEDLTGAGF
                     AGWQKPGAPLLFSRRDGSFFHLQNLLMVRAHDAGVVIALVDPALTSRCCSRCGEQGIR
                     RGKRFTCPRCGYTDHADVNAAFNIAAAPGIGRPAIPASSHRQRG"
     gene            complement(4087..4914)
                     /locus_tag="PLV90_01015"
     CDS             complement(4087..4914)
                     /locus_tag="PLV90_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95722.1"
                     /translation="MRSLTAKETYIRDVARLLSGFIASGNTTDLLGYLTAEGGLPGAD
                     PELTGAFTGVVREFAAADPDDRRLLWDLCIMLADIPPEGAPASDLHELLISCGIRGIG
                     TIGSLSPACAEPALTRLAEASADPRQRIREAAGMGVRDILSRQRDTVIPELARWVEGG
                     SWLTMRAAIMGIVEPDLLGEPDIPTAAFHLHRKVLIRIYTAKERQSEAFGALRATLGS
                     TLALVIAALPGIGFEYLRQLATLDDPDIRWIVRENLRESGLQKRYPETVRHIRAQIG"
     gene            complement(4949..5434)
                     /locus_tag="PLV90_01020"
     CDS             complement(4949..5434)
                     /locus_tag="PLV90_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOF95723.1"
                     /translation="MAEERLKIVIFGSFNAGKSSLIQALDPRSRHVEAASDEGPTTVA
                     FDFGRLQVRDRAVYLFGTPGQERFEFVRQILSRGMDGAIIVVDATTGVDAMTRHLYNQ
                     LKDFGVPLAFMANKCDRPDAVPEAVRRELPGEMVHPISARNGENVHAALDAFVATLVA
                     R"
     gene            5602..5955
                     /locus_tag="PLV90_01025"
     CDS             5602..5955
                     /locus_tag="PLV90_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95724.1"
                     /translation="MGSEETDTVAQEIMATLDTLFLAEKRARLQVSALEDRQYALATT
                     FRMVQEMEADSAIEEALSGFGFGYYTIDDDAELWISEEYGLMVFLSFTAPDGRYYHYR
                     IVSFDVIGGDGEEAG"
     gene            6023..6553
                     /locus_tag="PLV90_01030"
     CDS             6023..6553
                     /locus_tag="PLV90_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOF95725.1"
                     /translation="MERSVKRLMEDREKRREENIGKYIEQLCDESTPYRLRAAEALGG
                     CADTRAVEPLIAALHDPENEVRWVAAQALGKLCDARAVEPLLPLLADPDRWVRRGTAW
                     SLGEIGDPCAVEPLILLLTDSRKDVRIAVADALGKLHDARAVGPLTAALECEEEPEVR
                     TAIRRALRDITGEMEF"
     gene            6562..9399
                     /locus_tag="PLV90_01035"
     CDS             6562..9399
                     /locus_tag="PLV90_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOF95726.1"
                     /translation="MRLFRYYPEDFGVLTVKVVHMDLAFDVYVDHTRVVSALTAETLD
                     APIASLALNARDLDILRVACDGHSIAHAYDRGAAILTVTFDPPVPPRTRFTLDTETIC
                     RPSSHTLEGLYYDGSCPGGPPVQITQCQQWGFQRLVPCLDDMTAKCTYTTTIAADDGY
                     TNILSNGDPAGPRYLVAPGRSVQEYRNTTTPMAPYLFFLGVGCYDTHRREFEYPDGRA
                     FSLEILAKPGFAPDEPPERALDILADAVMWVHLFTGPEPYRDRKTRQEIWRLIRVRDA
                     AKRAGDRESLAETREALAGLVATVTPGYAYTGSVYREIAMQNSDFGGMENVGNTTIAA
                     NRIMPYPEATDPAFEYMVRVKVHEYYHNENGSEVTGRSPFEIWLNEAVTVHIENRHHA
                     FFFGEAYSRLQTVLDLLDPVAGTLVLDRGAGSMPIEPDGFDDPNDLITGVTYVKAPEF
                     VRMIETLMGKETFAVGLALYHDRFRHKNASTDDWIRCMEEVSGQDFTGMAAVWLKEKE
                     YPVLTVAPSYDPAARRFTLACRQNRSPGGRPREFPFRFALADADGRDIAERTVRIAGE
                     EEEVVIDDVDRPAFLSLNRGYSFYGKVVYRPPEDELYLQAAKDADIVGRFTAFNALAG
                     HEMLRLLEDPGAAVSSRFADLCAGLLADDALMEEAGGQFLTIFPAVDDERFAHRYRAL
                     HDARKKILAAVGARHADTLLAVYRQAAVDLPSGDGYLEDEARAIRRRQRKNTCLAILA
                     ARDAPEIHRLLAGQFREAAAATDRLTAFGLILESSAPERLNILAEFCAASAKNPVAWE
                     SFLAVVAGSDCDDIVEILRRIESSPAFSVEQAGEQRALYGRFALNRKRSLETEEGRAY
                     LGEVLRRLARVNEYSTARALDAFAFIDAMEPRHRVPVAGVLVGLLASLDPGKHPAIFN
                     NARRFLLGAPEAVRAYEAEHGEIPALRKDTS"
     gene            complement(9400..10239)
                     /locus_tag="PLV90_01040"
     CDS             complement(9400..10239)
                     /locus_tag="PLV90_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOF95727.1"
                     /translation="MKLTVLVDNATLTDRYFLAEPGLSIYLEDGATRILFDAGYSGIV
                     VSNARRMGIDLLRVGEIVLSHGHLDHTWGLDALLRLHTEAIIEGRERIEPTFIAHPDA
                     FLTRSFGGAGEIGCHLSVEELFRHGKVLLTASPLWLTENLVFLGEIKRRFDFEQMPSI
                     GYIYTPDGIRDDTVADDTALACRTPEGLVIITGCAHAGICSIVEQAREVCDEDRIADI
                     IGGFHLLNPSPGRMQGILDHLAEVRPSVLHPCHCTDLAAKIALAKVADVRDVGVGLHL
                     EYG"
     gene            complement(10456..11115)
                     /locus_tag="PLV90_01045"
     CDS             complement(10456..11115)
                     /locus_tag="PLV90_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95728.1"
                     /translation="MHEVPDTLAIDVVLLPPGPIMEMAIGANRTLLAGNPGGGIRLDG
                     EDCLPHVSVAMLPARREDMPEIIAKMDRIARRCSPMTMTIDAVAKHRSSTGETVSAFH
                     ILRAEILALFHKTVMNVLAPYATFPAGPPIFFEEASAPSIDCFLRFPETSAYERYSPH
                     ITLGFGDLPELLPGIDLPVRFEVTKAAICHLGSHCTCRRIIAGFDLGTGASAAHGRPI
                     R"
     gene            complement(11192..>11473)
                     /locus_tag="PLV90_01050"
     CDS             complement(11192..>11473)
                     /locus_tag="PLV90_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95729.1"
                     /translation="CWQEVFRSQAGFIFPDGILRPRYEGKEMETTQWLVTLTFRVTVG
                     GVRSKDDSVAAGLEQLKQSLNSGEDVPMEATVRRITNIVEEEPIFGVRW"
     assembly_gap    11474..11573
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11911..13179
                     /locus_tag="PLV90_01055"
     CDS             11911..13179
                     /locus_tag="PLV90_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HOF95730.1"
                     /translation="MIQEPATAPGRSMGRRTIGWISGMFIILLALIPAAAAGAPTVIV
                     SNYTVTPAVLMPGDEGTITVVLTSTASMAPGSPLDLRVDTGGVNTSPITPDNAYIENV
                     LLRSREIEVLSGSFQDVGEIGPGQSLPITFQVRAPGEEGIYFPEVWVSVRDATGVRYP
                     IPVNVGSAYALAKKPSLRVERTVPASVDPGDSFGVTLTLYNDGEASANDISVGINASS
                     DSIASTSPESYYIPVLRPGEEAALNLSFGTDPGAPLGLLPVVATVDYRSADLAVFRQV
                     ETIGIPIVGRADMSVASIRTEPARITAGDPVGLTIRIENTGTADATSVRATIGGLSLS
                     GTKEAFLGTIEPGNDGPAVFNLQAGEEGDYPYTLAIQYTDDYGAHTTEQALNLVVAGS
                     DRAPALIIAVVAIVVVIAAALYWYRRRKEE"
     gene            13176..14390
                     /locus_tag="PLV90_01060"
     CDS             13176..14390
                     /locus_tag="PLV90_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOF95731.1"
                     /translation="MTFPMFTGLRVSAFLAWKSIERGSKGTLALTIMIIALIFVNLIF
                     LPSIISGVVRAFDDQSIDFDFGNLVIEPREGDQYIDDAANLQRKVDRIPGITGTSPRI
                     AAGVTYFYRGRNAASTLYGIKPADERAVTKIADRMVEGEFLSNGDTDAIIMGTTLAGR
                     EGEEAGGLPSLRGVVVGSPVEVVYPNGEARTYRVKGIYETQSISIDASAFVTAREMNG
                     VLGLHDQASIILVKTDVNGREAEYKTELLSYGIQEDIWTYQEKSGGFVNQAIQSFDII
                     TAISALVSLIIAVVVVFIVIFINTVNRRRQIGILKAIGISQGVIVNSYILQVLFITAV
                     GTLAGIALTAGLTIYLTAYPLAFPGGPVSPAVEASAVIQSIASLFAVSVAAGYIPAWR
                     VANEDILTAIRG"
     gene            14394..15104
                     /locus_tag="PLV90_01065"
     CDS             14394..15104
                     /locus_tag="PLV90_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF95732.1"
                     /translation="MIVAENLTRVYAMGRVEVQALRGVSLEVGKGEFVGIMGASGSGK
                     STLLHILGLLDRPTSGRVTIDGTDVLALSDHRRTLFRLNRLGYVFQDYALIGELTALE
                     NVYLTSLVRGDPEEEYLRKSTAILERVGLAGRTNHRQSELSGGEQQRVAIARALVNNP
                     SILLADEPCANLDSQASKSILDLFARLNEDLDQTIVMVSHEEWHKEYFCRVVTLRDGL
                     IGDTEECRREGPPPGRTA"
     gene            15254..15430
                     /locus_tag="PLV90_01070"
     CDS             15254..15430
                     /locus_tag="PLV90_01070"
                     /inference="COORDINATES: protein motif:HMM:NF018096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HOF95733.1"
                     /translation="MQNVRTFRCKDLGLACEFEEKAEGENELMKRIEGHVQTAHQMNL
                     EHPEVQERVRKAMK"
     gene            15557..16111
                     /locus_tag="PLV90_01075"
     CDS             15557..16111
                     /locus_tag="PLV90_01075"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOF95734.1"
                     /translation="MDEAARRRWQHPESILEGIGLAAGATFIDVGCGDGFFALPAARI
                     VGPHGRICGIDIDAGALDTLKKRARGEGLENIALHRGMAEEIVLCEGCADVVFFGIDL
                     HDFTDPVRVLLNAKRMAAPGGVIVDLDWKKEPLPFGPPVAKKFTTEHAASLMAKAGLT
                     VAAVADSGPYHYIITAVPGPGRVR"
     gene            complement(16127..16726)
                     /locus_tag="PLV90_01080"
     CDS             complement(16127..16726)
                     /locus_tag="PLV90_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95735.1"
                     /translation="MKTTSSKLLFPALIVVLGICLCMTAGCTSSSSDTTTPEAATTAP
                     AAGTTVSGAARGSVTAVPFETLITFLPAAPAGWTAEEPVGITMAVEDGQWTWSSRDYT
                     KGDAQAAIVIQDSAYYNVGQWQMWNTFTSIETTEGYYKSKTIGGHPAWEVFSKPDSYG
                     TWVGVNERFMVYVSVDGGSKQDLDAFVNAIDYGGIANLK"
     gene            16941..17402
                     /locus_tag="PLV90_01085"
     CDS             16941..17402
                     /locus_tag="PLV90_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HOF95736.1"
                     /translation="MIQVKRIHGIVIAGLLVACMLMAGCTSQPENTTVTPSPAATTAV
                     TPSPAERFAFNETDDNKAVTLPAGSEIVISLDENPTTGYSWNVTSSRGLSYVNDTFIP
                     PKTDLVGAGGVHEWQYRAAEKGSAEFSAIYMQPWMNVTGSETTFSMTFTIE"
     gene            complement(17508..18242)
                     /locus_tag="PLV90_01090"
     CDS             complement(17508..18242)
                     /locus_tag="PLV90_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOF95737.1"
                     /translation="MATVDPGMQAVRAPVPGRGRINACSLPAVIPVPLGPTTAFIVRD
                     RGVLLIDTGNPGDEAAILAAMRRAGIRPDDLSLILVTHGHPDHYGSADALRDRTGAPI
                     AVHAADAGAVRCGVAGRFSLARIVPHQIGFMAGKTGSIEPDIVIEGAADLAVFGIRGR
                     VIPTPGHTPGSVSVFIAGGAAIVGDLLSALLPGGRPRLPFRTGDPAAARRSIRALLAL
                     GPERIYAAHGGPWAGAEVSRWFGNEW"
     gene            complement(18448..20640)
                     /locus_tag="PLV90_01095"
     CDS             complement(18448..20640)
                     /locus_tag="PLV90_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor EF-2"
                     /protein_id="HOF95738.1"
                     /translation="MTRRKKMVERVTDLMDKPEHIRNIGIVAHIDHGKTTLSDNLLAG
                     AGMISEELAGRQLFMDSDEEEQARGITIDASNVSMVHEYAGEEYLINMIDTPGHVDFG
                     GDVTRAMRAVDGAVVVVDAVEGTMPQTETVLRQALKEGVRPVLFINKVDRLINELKVD
                     EQEMQIRLARVIDKVNKLIKGMNEKMYNEGWKLDAAKGTVAFGSALYNWAVSVPYMKK
                     SGVSFRDVYEKCNTGDMKWLAKSSPLHAVLLDMVVKHLPNPLQAQDRRIHIIWHGDYT
                     SSEGKAMLTCDPNGPVCMMVTDITFDPHAGEVATGRLFSGTLRRGTECYIMGAAKRAN
                     RLAQVGIFMGAERIEVEELAAGNIAAVTGLKDAIVGSTVTTLVDMTPFESLKHYSEPV
                     MTVAVEAKNMKDLPKLVEVLRQVAKEDPTVQVSINEETGEHLISGMGELHLEIITGRI
                     KRDKGVDIITSPPIVVYRETITGRAGPVEGKSPNRHNRFYLELEPMNPEVVKLIQQGD
                     VSMTQQAVERRDALIAAGMDKDEAKNVKAIEGTNMFIDMTKGIQYLNETMELVLEGWR
                     EALHGGPLADELVQNLKIRLVDVKLHEDAIHRGPAQVIPAVRSAVKAGLLMGGDSLLE
                     PIQKLQITVPSDQMGAATSQIQGRRGQVFDMLSEGDTMTIVGRAPVAELFGFAGDVRS
                     ATEGRAMWSTEFAGFELVPSGIVDDVVKGIRRRKGLKEQLPRPEDYLA"
     gene            complement(20667..21260)
                     /locus_tag="PLV90_01100"
     CDS             complement(20667..21260)
                     /locus_tag="PLV90_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOF95739.1"
                     /translation="MEEAAEAGIQDQKLLFNRWDMSEVEIKDPSLARYVNLHAMIVPH
                     SCGKLAGQQFNKSNMLIVERLINKMMQTEHNTGKKELAIRIVKDALEIVNKKTKKNPV
                     QVLVDAVENAGPREETVRLKYGGINVPKSVDTAPQRRVDTALRFITDGVRQASHKKKK
                     TVSEALAEELIAAANGDTRSYAVSKREERERIAKAAR"
     gene            complement(21266..21694)
                     /locus_tag="PLV90_01105"
     CDS             complement(21266..21694)
                     /locus_tag="PLV90_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOF95740.1"
                     /translation="MGNGKFAARKLKRDAKRDRWHDPVYARRQLGLDVKSDPLEGAPQ
                     ARGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGRQVTAFAVGDGAINFIDEHDEVEIEG
                     IGGRLGRSMGDIPGVRFVVTKVNNVSLSEMVIGRKEKPRR"
     gene            21868..22281
                     /locus_tag="PLV90_01110"
     CDS             21868..22281
                     /locus_tag="PLV90_01110"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOF95741.1"
                     /translation="MTTRIYKEVFFEATHRLIHYRGKCFRLHGHQWRVEVWIEGTADE
                     RTGIVLDYNCIKEVVGRFDHQVILNVGDPMAACIEAFHPVVTTPGDPTSELLAGLIAD
                     MIDAEAARQGLDARVKKIRVWESTSCYAERTYDRQ"
     gene            22268..22876
                     /locus_tag="PLV90_01115"
     CDS             22268..22876
                     /locus_tag="PLV90_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOF95742.1"
                     /translation="MIVSEIFRSLQGEGRNQGRPCTFIRLAGCNLRCAWCDTSYAWEG
                     GMRMSVAEVLDRVWLLNGKQICITGGEPLLQRDEVAGLLEKFDRHGYTVEIETNGTQD
                     FRDMQAFASICMDVKCPSSGEQSDLSLLPFIGRRDCVKFVVADENDFLYARAVMARYD
                     IRGEVIITPVEGSDYRAIADYIVEENLPVRFQLQLHKILGMK"
     gene            22876..23535
                     /gene="queC"
                     /locus_tag="PLV90_01120"
     CDS             22876..23535
                     /gene="queC"
                     /locus_tag="PLV90_01120"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOF95743.1"
                     /translation="MRAVCLLSGGMDSATLAYAAKDMGYDIIALHFAYGQRTEKRERA
                     CARTIARMLDAAEFVEIGLEHFKKIGSSSLTDESIPIDEYTEKGVPATYVPFRNANLL
                     AIATSLAEARHAEAIFIGVQTGDYPGYPDCRPEFIEAFQHAIDIGTAADPPITLMTPF
                     VRMTKADILRVGLRLGVPYEETWSCYQNNDRACGVCSSCHYRQTAFRELGLCDPIAYE
                     D"
     gene            23538..24032
                     /locus_tag="PLV90_01125"
     CDS             23538..24032
                     /locus_tag="PLV90_01125"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOF95744.1"
                     /translation="MEIYRGKRLTVEKREITLPNGKRKERVVVHPGGAVAILPVEGDD
                     CYLIRQYRYAVDDYIFEAPAGTIDEGEEPHETARRELIEETGMKAEVFTPKGWIYPSP
                     GYTDERIWLYIAERLSPCHDYDMDEDEVIEVVRTPLRDLAAMIADGRIVDAKTICLIC
                     RCFL"
     gene            24168..25439
                     /gene="prf1"
                     /locus_tag="PLV90_01130"
     CDS             24168..25439
                     /gene="prf1"
                     /locus_tag="PLV90_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor aRF-1"
                     /protein_id="HOF95745.1"
                     /translation="MEETHEMDTARKRYEFKKMLERLAEKEGSGTELITLYIPPDKQI
                     YDVTAQLRDEFGQCANIKSKQTRTNVQSAISSILSRLKYYKRPPENGMAVFCGTINIG
                     GDRTDLECEIIEPPEPLNTYLYRCSSSFELEPLQQMLGEKEVYGLIVIDRREAYIGFL
                     RGNRIEAVRGATSTVPGKQRKGGQSSVRFQRLRLIAINEFYKKVGDLANDIFLAEKDF
                     FERFKGVLIGGPTPTKEEFEAGGYLHHELQKRIIGLFDVAYTNESGLAELVENASDAL
                     KGLDIIKEKNIMNRFLQELVKDDGPAAYGEESVRKNLELGSVDTLLLSDKLRRARLKL
                     ACPNSDYTEERTISIEPGKHIKDIDFGTCPNDGASLYVAEEVDIIDELTALADQSSTT
                     VRIISDDFEEGSMLYNAFGGIAAILRYRTGY"
     gene            25439..27106
                     /gene="argS"
                     /locus_tag="PLV90_01135"
     CDS             25439..27106
                     /gene="argS"
                     /locus_tag="PLV90_01135"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOF95746.1"
                     /translation="MFQEKYHQVERMLRACTGEEDVLLTIGGDHADIASTVAFKLAKK
                     ERRAPQKIAADIAARLSADPDLGDIRVGAVGPYVNFRFGPSLLSEAVRAATKPGYGNL
                     PRRTERVVLEHTSANPNGPLHVGHIRNTVIGDTLVRAFRKAGYPLEAQYYLNDMGRQI
                     AIVSWGFDHLGLARGEGEKADHYVARVYVAANRAIRENPEITEEIDRLMQQVERGDAE
                     TVTKFRAAVSLCAEGIKATLAALNARHDRFVWESDFVRIGDVDRIIERVRRLPQAHED
                     EILWVDLSEQGFEKKYILRRSDGTSVYSTRDLAYHTWKARNFDRMIDILGADHKLIGA
                     QLRATLELLGEQPPDIVYFEFVSLPEGSMSTRAGKFVSADELIEEVTAKAYDEVTVRR
                     PELPEGERRAIAGSVAVAAIRYDIVKVSPEKSTVFDWREALNFERQSGPYIQYAHARA
                     CSILDKAGAVSMPAYHYETEHEVALARHLARFPVVVEQTVTELRPHLLASYARELADL
                     FNAFYHYDPVLKSEGEARNSRLALVDAVRNTLQESLATLGIDALRSM"
     gene            27087..27980
                     /gene="twy1"
                     /locus_tag="PLV90_01140"
     CDS             27087..27980
                     /gene="twy1"
                     /locus_tag="PLV90_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-demethylwyosine synthase TYW1"
                     /protein_id="HOF95747.1"
                     /translation="MHSEACKALRKQGYQFVSPASSAAVKPCMWNKRALRGGAMCYKA
                     QFYGIESHRCVQMTPTLRCNQQCLFCWRSFEHEVVEEEECPPETIIESLPRLQKKALA
                     GYKVSPYVAPERFAEALRPTMVAVSLSGEPTCYSQLPNLIDGLNAGGYTTFLVSNGTR
                     PDVLARCRPYQAYVSLDAPDRETYLGLCRPREDYWDCIQESLAGLAGRRSAIRTTVVR
                     GYNDFAPEGYAAIYQDSGARFVEVKGYMYLGYSRKRLQRDQMPEHSDVRAFAEKIAEH
                     CDYTVKDESPASRVVCLERKI"
     gene            27977..29572
                     /gene="sepS"
                     /locus_tag="PLV90_01145"
     CDS             27977..29572
                     /gene="sepS"
                     /locus_tag="PLV90_01145"
                     /EC_number="6.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043816 - phosphoserine-tRNA(Cys) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-phosphoserine--tRNA ligase"
                     /protein_id="HOF95748.1"
                     /translation="MRFDVEEFKKRAREDFEHAWHEGPSVLTPPGISGQYPRLKYTRA
                     TAHPIFEITQRLRETYLSMGFDEAMNPLIVEEADIYRQFGPEAMAVLDRVFYLGGLPR
                     PNVGIARKQIEGIEAILGRTITPPVEERLRETLHGYKKGTIDGDDLTHELAAVLEADD
                     AAVVHILDAVFPEFRALAPESSRNTLRSHMTSGWFLTLSAIWEKRHLPIRLFSVDRCF
                     RREQEEGPTRLMAYHSASCVVAGEDVTLEEGKAVSEALLSAFGFTEFRFQPDEKRSKY
                     YMPGTQTEVYARHPVLDWVEVATFGIYSPSALAEYGIGVPVMNLGLGVERLAMIAYRS
                     NDIRELTYPQFFPQTLSDRDIAGAVHLRTEPASTTGKRMAEAIRATAAEHATAPGPCA
                     FTAWKGEIAGREVEVIVEEPESNTKLCGPACANEVYVHDGSVLGVPDTEKWASVRREG
                     VSTGITYLDAVAGLAAARIEEAARCGEGTTVQVKMSKLPSDVNLRIEEYAMRYITDHN
                     KKVDLRGPVFLTVRSVIPEQSTR"
     gene            complement(29547..>29923)
                     /locus_tag="PLV90_01150"
     CDS             complement(29547..>29923)
                     /locus_tag="PLV90_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HOF95749.1"
                     /translation="KDDLLHLTSNLNVGVMEGNKVIEIKNTVVDKGRAALNWVSQHAW
                     DFILAIGDDRTDEDLFAAMPPDAYSIKVGLAPSRARFNLVSQRDVLPLLRRCIECGRE
                     GVTRKREKPGREKSLIGSTAPV"
BASE COUNT         5507 a         9437 c         9504 g         5375 t
ORIGIN      
        1 ggtgaattga cggccgatca gagcaccccg cacccggaga ggcggtagag cctgcggatc
       61 cgttcagccg cctcatccga gaccgccttc tcgatccgga tgaggatggt ctcgatgtcc
      121 tcctcgggca tgcacccgag gtcttcgcgg ccggaaagga tccggtcggt gaggtatatc
      181 cagtcctcat ccgagaggcg gccgacccgc tccgcaaagt cttcctcgtc cgctgcgatg
      241 tggttggaga ccggcggcag tatcgagtgg aactcgtgtc ccgactcagg gcagaggatc
      301 ccgctgtatt ccggggccag cttccggagg attgcaaaat ctctctcgtc gaatttgcgt
      361 gccatggttt cgttcactcc cgggagcggc ggctacaaga acgttcgccc gccgcgctgt
      421 tacgattccg caaggagcct gcccgcccgt gctatgttct cggtgtcgag ttcctcaaag
      481 aagagggtga cacgttccgg atcgacgccg agcgtctccg tgatcgccgc ggtgatctcg
      541 gcggcaagtc ttcgtttctg ctcaagcgtc cgcccctttg ccatacgaat ggttacaacc
      601 ggcatgatga aaaatgtggg atgggacggt atttatcggt gtgcggtatg aaccggaggc
      661 cccatccgta agggcgggga ggggttcacc ggtacacaag ggcgccgggg cggacgacct
      721 gaaacgtggc ggtgccgggc ggcaaacccc ccgcgatcgt gagccccacg atatcccgga
      781 ccgcctgccc gaaggcctcc cgctctccgg gcgtcaaccg ctgccgggct ccgtccccga
      841 gtcggtctgc cgcccgggcg atctccgtat cgagcctcgc cgcgagcgcc tgatcggtcc
      901 tgtcctcgcc ggcgatgccc tggaggagga gcacctcgga cccgggccga cgacccggga
      961 cccccgccgc gggcgcgtgc aggtcggcga tgtcgtcggc gatctgcatc gccttcccgg
     1021 taaagaggcc gaagtctgca aacgcagcga cgacatcctc gtcctcgtcc gccgccatgg
     1081 caccccaggc ggctgcgagc gcgaagaggc atcccgtctt tcttgcgatg atcgcatcat
     1141 agagctcgat cgacgaaagc ccggatcccc cgagcatctc ccaggccgcg ccccgggcca
     1201 tgctctgctg ggccgacgcg agcattgcca cgtattcggc accgcagggg gcggcgagcg
     1261 cgtacgggag ggcgaggacc ccgacggcat cgaggaccgc ccgccccgtc ccccgggtga
     1321 ggtggtggga gggcagcccc cgccggagga tgtcgccgtc cagcatatcg tcgagaatga
     1381 ggcttgcggc gtgggcgagt tcgacggcac aggcaaggtc gagcgcccgg cgggtcggcg
     1441 cctttctgcc cgtgagggcc gcgtgggtat ggaggaggag cccggcccgc atcctcttac
     1501 ccctgagaag gagcgggacg acgccggggt cgatcgaacc ctcatctccc gtcacctccc
     1561 cgatccgtcg gttgacggca ggtctgatct cctcaaggaa ctcataaaac ggcatcatgg
     1621 ctcatcactc ctccggaggg gtcgatgcac cccgttaagg cgcggagaac gtaaaaaggt
     1681 gtgggtcgat ccctctctcc ggtaacttca tagtcctgaa acgccttccg ggccctcccg
     1741 gtggggttgc gccgggatga ggaggatgcc gggtgggcac cgagaggagg gtgggacgtc
     1801 tggaagaggg cgatgcgttc cgtgactggc tcgcgggcat ccttgccgat cgattgaccg
     1861 ggtggcgtgg aaagatccgg gtctaccgga tagaacctgc atcccatgtc gtctgccggt
     1921 acgagttcgc cggcgggcca agcgtcgtcg gtaaattttt cggtgcaccg accggagcga
     1981 gaacccgcta caacacggat gcagcgatga agaacgagtt tttaaggctc cggcgtgtat
     2041 cgggctggat ccgcgttccc catgccatcg ccacgaacaa ccggttccat tccgtcctcg
     2101 tgaccgagca tgtccccggc ccgaccgtgc gagaactcct cggtgcaggc gtatccctgt
     2161 acgaccccct gaccggtgtc gcgcacctgc tccgccgact ccacgacagc acacggactt
     2221 cctgcaagcg ggagcgcgat tttgcctact accatgcgat cctcgatcag aacaaccttt
     2281 ccggttcccg gaggaagcgg ttcgaccgcc tgctcggagc gtggtggcac agtgcgcgcc
     2341 tcggcccgga gggatgcatg gtccatggcg atgcaacgct cacgaactac ctcttcaacg
     2401 gagatacctg tgctatcgac tttgaaggat cccggaacca cacccacccg atacgcgatc
     2461 tcggcatcct ggctgcagag ctaagaacgt cgggcgcgag catctcgacg gccgaagact
     2521 acatcggcca cctgctctgg cattatagca ggggtgaaga agagttccgg cgctataccg
     2581 ccgtccttcc cttcttcatg gctctcggat acctccggat tgcacggttg ccctggcggg
     2641 cagcggaacg tgactggctt ttagaggagg cggaggcgtg tctctgtgcg atccaccggt
     2701 agcaggggtt ctcttcgact gctacggaac gctcatcgat atcctgaccg atgaacgcga
     2761 taccgagacc taccgaaacc tcgcgcgatg gctgatctac cagggggtca tggtcgctcc
     2821 ggatgacctg cgggttctct acacctgcca tgtcagggag gcgatcgagc agatcgggga
     2881 gccctacccc gaggtccggg tcgaggagat ctttgccggc atctgcgccg aacatgccat
     2941 ccggaagatc gatgccgggc ggctcggtgt cgagaccgcg cgggcgttcc gggccgcatc
     3001 gctccggcgt ctcggcatca tcgaggagag tcggaggctg cttgacctct tctgcgcgga
     3061 gaagctcggt gtcgtctcga acgggcagcg ggtcttctcg gagtgcgaga tgcgggcgtt
     3121 cgggctctat gatcgcttcg actttgtcat cttctcttca gatctcggct accagaaacc
     3181 ggatacccgg atatacgccg cggcgctcgg gcggatgagg ctttccgccc cggaagtcct
     3241 cttcatcggg gataatcccg aaaacgacgt cgcggcgccc cggaggttcg gcatgcgggc
     3301 gctgcacgtg gaggaggcct ggaggcgtta tagcgattga gatggcgggt ccggtgggta
     3361 tatggtggcc gggggaccat tcctgtccat acaccagaag cgcatgagac cgaagacctg
     3421 cgaccccggg gacgatgccg tttatcagag gtagcccgcc gcccggcgac tggatcggtg
     3481 tcgacctgaa cgccaccggc tctatcgccg tcgtggccca tccggcgagc ggaggcgtgg
     3541 tgatgctcgg cgagcagccg gagggtgccg gcaccccgga gcggtgcagc ggggggaaac
     3601 gtaaggcgct cagggcggcc cgggaccggg agagcagcgc ccggggggat ctgaaccgga
     3661 ggattgcaag agagatcgtg aagatggctc gaaacctcga gtgcggcatc aaactcgagg
     3721 atctcaccgg cgcgggattt gcggggtggc agaaaccggg cgctccgctt ctgttctccc
     3781 ggcgcgacgg atcgtttttc cacctccaga acctcctcat ggtccgggcg cacgacgccg
     3841 gtgtcgtgat cgccctcgtt gacccggcgc tcacgtcgcg gtgctgcagc cggtgcgggg
     3901 aacagggtat ccgcaggggg aagcggttca cctgcccgcg ttgtggctac accgaccatg
     3961 ccgacgtaaa cgcggcgttc aacatagcgg cggcgccggg catcggtcgc ccggccatcc
     4021 ctgcctcttc ccatcggcag cgcgggtaaa cccgcccggt ggggtagtgg cggccttgta
     4081 tcttctttac cctatctgcg cccggatgtg ccgcaccgtc tcggggtaac gcttctggag
     4141 cccgctttcc ctgaggttct ccctcacgat ccaccgaata tccggatcat cgagggttgc
     4201 gagttgccgg agatattcga atcctatccc gggcagggcg gcgatgacca gggcgagcgt
     4261 ggagccgagt gtcgccctga gcgccccgaa cgcctcagat tgccgttcct tcgccgtgta
     4321 gatacggatc aggacctttc gatggaggtg gaaggcggcc gtcgggatat cgggctcacc
     4381 gagcaggtcc ggctcgacga tccccatgat tgcggcgcgc atggtgagcc aggagcctcc
     4441 ttccacccat cgtgcgagct ctggaatcac ggtatcccgc tgccgggaca ggatatccct
     4501 gacccccatc cctgccgcct cccggatccg ctgtcgggga tcggcggacg cctccgcaag
     4561 gcgggtgagc gccggttccg cgcacgccgg ggagagggag ccgatcgtcc cgatgcccct
     4621 gataccgcag gagataagga gttcgtgcag gtcgcttgcc ggagcacctt ccggggggat
     4681 gtcggcgagc atgatgcaga ggtcccagag gaggcgtcga tcgtcggggt cggcagcggc
     4741 aaactcccgt acgaccccgg tgaacgcccc ggtgagttcc ggatctgccc cgggcaggcc
     4801 gccctccgcg gtcaggtagc caagaagatc cgttgtgttc ccgctcgcga taaagcccga
     4861 gagcaggcgg gcaacatccc tgatgtaggt ctctttcgcg gtcaatgatc tcaagagctc
     4921 aatgacccgc agacagtaaa aatgatcgtt accgggcgac cagggttgcg acgaaggcgt
     4981 cgagtgccgc gtggacgttc tcgccgttcc gtgccgatat ggggtgcacc atctcccccg
     5041 gaagttcccg gcggacggcc tcgggcaccg cgtccggacg gtcgcacttg ttcgccatga
     5101 acgcgagcgg taccccgaag tccttcaact gattgtaaag atgccgcgtc atcgcatcca
     5161 ccccggtggt cgcatccacg acgatgatcg cgccgtccat ccctcgcgag aggatctgac
     5221 ggacgaactc gaaccgctcc tgccccggcg tcccgaagag gtagaccgcc cggtccctca
     5281 cctgcagcct gccgaagtcg aaggcgacgg tcgttgggcc ttcgtccgag gctgcctcga
     5341 catgccggct ccggggatcg agcgcctgga tgagactgga tttgccggcg ttgaacgagc
     5401 cgaatataac gatcttgagt ctctcttctg ccatcaaacc tgtattggca tccggatggt
     5461 ttagagcttc gctgccgggg ctcgagaatg ggatggatgt gcaaccccgg tctttttacg
     5521 aacaggcggt tgttcacggc gtggatcgga gatccccgcg cggtgatacg ctgatatacc
     5581 ggcacgccca aagtaccggg tatggggtcg gaggagacgg ataccgtcgc gcaggagatc
     5641 atggccaccc tggacaccct ctttctcgca gagaagcgcg caaggctcca ggtatccgcc
     5701 ctcgaggatc ggcagtacgc ccttgccacg acgtttcgga tggtccagga gatggaggcc
     5761 gacagcgcca tcgaggaggc cctctccggg ttcgggttcg gctactacac catcgacgac
     5821 gatgccgagc tctggatatc ggaggaatac gggctgatgg tcttcctctc gttcaccgcc
     5881 ccggatgggc ggtattacca ttacaggatc gtctccttcg acgtcatcgg tggggatgga
     5941 gaagaagcgg gataaggata ccgggcagaa taatatacca taacgacatt aatttccgat
     6001 agaaacaggc ggagggatta agatggaacg ctcggtaaaa cgcctcatgg aagacaggga
     6061 gaagcggcgc gaggagaaca taggcaaata catcgagcag ctctgcgatg agagcacgcc
     6121 ctaccggttg cgggctgccg aggcgctcgg agggtgcgcc gacacccggg cggtcgaacc
     6181 gctgatcgcc gccctgcacg acccggagaa cgaggttcgc tgggtcgcgg cgcaggcgct
     6241 cgggaaactc tgcgatgcac gggcggtcga acctctcctc cccctcctcg ccgatcccga
     6301 ccggtgggtg cggcgcggca ctgcctggtc tctcggggag atcggcgacc cctgcgcggt
     6361 cgaacccctg attctcctcc ttacggatag caggaaggat gtcaggattg ccgtcgccga
     6421 tgcgctcggg aaactccacg atgcccgggc agtcggcccc cttaccgccg cgctcgagtg
     6481 cgaggaggaa ccggaggtcc ggacggccat ccgtcgtgca ctccgcgata tcaccggcga
     6541 gatggaattc tgacaccggg tatgaggctt tttcgctact atccggaaga tttcggggtg
     6601 ctcaccgtaa aggtcgtcca catggacctc gccttcgacg tctacgtcga tcatacgcgg
     6661 gtcgtctcgg ccctcaccgc cgagaccctc gacgcaccga tcgcatccct cgccctgaac
     6721 gctcgcgacc tggatatact cagggttgcc tgcgacgggc acagcatcgc ccacgcttac
     6781 gaccgcggtg ctgcgatcct caccgtcacc ttcgaccctc ccgttccgcc gcggacccgg
     6841 ttcaccctcg ataccgagac gatctgccgc ccgagcagcc acaccctcga aggcctctat
     6901 tacgatgggt cctgcccggg agggccgccc gttcagatca cccagtgtca gcagtggggg
     6961 ttccagcggc tggtcccctg cctcgacgac atgaccgcaa agtgcaccta cacgacaacg
     7021 atcgccgcgg acgacgggta tacgaacata ctgtcgaacg gcgaccccgc gggaccgcgc
     7081 tacctcgttg cgcccggccg atccgtccag gaataccgga acaccacgac cccgatggcc
     7141 ccctacctct tcttcctcgg cgtggggtgc tacgataccc accggcggga gttcgagtac
     7201 cccgacggcc gggcgttctc cctcgagatc ctcgcaaaac ccggttttgc cccggacgag
     7261 ccgccagagc gtgcgctcga tatacttgcc gatgcggtga tgtgggtcca cctcttcacc
     7321 ggcccggaac cgtaccgcga ccgaaagacc cggcaggaga tctggcgact catccgggtg
     7381 cgggatgcgg ctaaacgcgc cggggaccgg gagtctctcg cggaaacgag agaagcgctt
     7441 gcagggctcg ttgcaaccgt caccccgggc tacgcctaca cgggctcggt ctaccgggag
     7501 atcgcgatgc agaactccga cttcggcggc atggagaacg tcgggaacac gacgatcgcg
     7561 gcaaaccgga tcatgcccta cccggaggcg accgatccgg ccttcgagta catggtcagg
     7621 gtgaaagtcc acgagtatta ccataacgag aacggctctg aagtgacggg gaggagtccg
     7681 ttcgagatct ggttgaacga ggcggtgacg gtccatatcg agaaccggca ccacgcgttc
     7741 ttcttcggcg aggcctactc ccgcctccag accgtgctcg acctcctcga ccccgttgcc
     7801 ggcacgctcg tcctcgaccg gggcgcgggc tcgatgccga tcgagccgga cggctttgac
     7861 gacccaaacg acctcatcac gggcgtcacc tacgtgaaag ctcccgagtt cgtcaggatg
     7921 atcgagacgc tgatgggaaa ggagacgttt gcagtggggc ttgccctcta ccacgaccgg
     7981 ttccggcata aaaatgcctc aacggacgac tggatccgct gcatggagga ggtctcgggg
     8041 caggacttca ccgggatggc ggcggtctgg ctcaaagaga aggagtatcc ggtcctcacc
     8101 gtggccccct cctacgatcc cgcggcccgg cggttcaccc ttgcctgccg ccagaaccgc
     8161 tcgcccggcg gccgcccccg ggagttcccg ttccggttcg ctctcgcaga tgccgacggt
     8221 cgggatatcg cagagcgaac cgtccgcatc gcaggcgaag aggaggaggt cgtcatcgac
     8281 gacgtcgacc ggcccgcgtt cctctcgctc aaccgcggct actccttcta cgggaaggtg
     8341 gtctaccggc cgccggagga cgaactctac ctccaggcag cgaaggatgc cgatatcgtc
     8401 ggccgtttca ccgcgttcaa tgccctcgcc ggccacgaga tgctccgcct cctcgaagat
     8461 ccgggcgcag cggtctcgag ccggttcgcc gacctctgtg ccggcctcct cgccgacgat
     8521 gccctcatgg aggaggcggg cggtcagttc ctgaccatct tcccggcggt ggacgacgag
     8581 cggttcgccc accgttaccg cgccctccac gatgcgagga agaagatcct tgccgctgtc
     8641 ggcgcacggc acgcggatac cctcctcgcg gtctaccggc aggcggcggt ggatctcccg
     8701 agcggggacg gctacctcga agacgaagcc agagcgatac ggcggcggca gaggaagaat
     8761 acgtgccttg caatccttgc cgcccgcgat gccccggaga ttcatcgcct gctcgccggg
     8821 cagttccgtg aagccgcggc cgcaaccgac cgcctcaccg cattcgggct gatcctcgag
     8881 agcagcgccc cggaacgcct gaatatcctt gcagagttct gtgctgcatc ggcgaagaac
     8941 ccggtggcgt gggagtcctt cctcgccgtc gtcgccggga gcgactgcga cgacatcgtg
     9001 gagatcctcc gcaggatcga gtcctccccc gccttctcgg tcgagcaggc aggcgagcag
     9061 cgggcgcttt acgggcggtt tgcgttgaac cgcaaacggt cgcttgagac ggaggaggga
     9121 cgggcatacc ttggggaggt cctccggcgt ctcgcccggg tgaacgagta cagcaccgcc
     9181 cgggctctcg atgcgttcgc gttcatcgat gcgatggagc cccgccatcg ggtgccggtg
     9241 gcaggcgtgc tcgtaggcct ccttgcctcc ctcgacccgg gcaagcaccc ggccatcttc
     9301 aacaacgccc gccgcttcct tctcggcgct ccggaggctg tcagggctta cgaggcggag
     9361 cacggggaga tcccggcgct ccggaaagac acatcctgat cagccgtatt ccaggtgcag
     9421 cccgacgccc acatcgcgga cgtccgcgac ctttgcaagc gcgatcttcg ctgcaagatc
     9481 cgtgcagtgg caggggtgga ggaccgacgg ccgcacctcg gcgaggtggt cgagtatccc
     9541 ctgcatcctc cccggcgagg ggttgaggag gtggaatccg ccgatgatgt cggcgatccg
     9601 gtcctcgtcg cagacctctc ttgcctgctc gacgatcgag cagatccccg cgtgagcgca
     9661 cccggttatg atgacgagcc cttccggagt cctgcaggcg agcgccgtat cgtcggcgac
     9721 ggtatcgtcc cggataccgt cgggggtgta gatgtaaccg atcgagggca tctgttcgaa
     9781 atcgaaccgg cgcttgatct cgccaaggaa gaccaggttc tcggtcagcc agaggggaga
     9841 cgccgtgagg aggacctttc cgtgccggaa gagttcctcg accgagaggt ggcacccgat
     9901 ctctcccgca cccccgaagc tccgggtgag gaatgcgtcg ggatgggcga tgaacgtggg
     9961 ttcgatccgc tcgcgcccct cgatgatggc ctcggtgtgg agacggagca gcgcatcgag
    10021 accccaggtg tggtcgagat ggccgtgcga gaggacgatc tcgccgacgc ggaggagatc
    10081 gatccccatc ctccttgcgt tggagacgac gatgcccgag taaccggcat cgaagaggat
    10141 acgtgttgcg ccgtcttcaa gatagatcga gagcccgggc tctgcgagaa agtagcggtc
    10201 ggtgagcgtg gcgttatcca cgaggaccgt cagcttcatg cgcaccgccc gcccggagtc
    10261 tcgccgatcg ccgccggcgt accgggaacg gcagggagaa ggggacgccg gaacgtcgcc
    10321 cgggccgtga gcagatccat gcagatactg gggacgtcgg ggctaagaag gttttcgggc
    10381 agagaaatcg ttccgggtcc atccatccgt acaggggaat acgtcagggt gctgctgcgg
    10441 taaccggccg atcgctcacc ggatcggcct cccgtgggca gcggaggcgc cggtcccgag
    10501 atcgaacccg gcgatgatcc gccggcaggt gcagtggctg cccaggtggc agatggcggc
    10561 cttcgtcacc tcgaacctga cgggaagatc gattcccggg agcagttcgg ggaggtcgcc
    10621 aaacccgagc gtgatgtgcg gggagtagcg ctcgtaggcg gatgtctccg ggaaccggag
    10681 gaagcagtcg atggatggcg cagacgcttc ttcaaagaag atcgggggcc cggccggaaa
    10741 cgtcgcatac ggcgccagga cgttcatcac ggtcttgtga aagagcgcga ggatctccgc
    10801 ccgcaggatg tggaaggcgg agacggtctc ccccgtgctt gagcggtgtt tcgcaacggc
    10861 atctatggtc atggtcatgg gggagcaccg gcgggcaatt cggtccatct tcgcgatgat
    10921 ctccggcata tcctcgcgcc ttgccgggag catcgcgacc gagacgtgcg ggaggcagtc
    10981 ctcgccgtca aggcgtatac cgccgcccgg gttgccggca agcagggtcc ggttcgcgcc
    11041 gatcgccatc tccatgatcg gcccgggcgg caggaggacg acgtcgatgg caagcgtatc
    11101 gggaacttcg tgcatggggc cggatcaggc ggcagtggta aaaaaaggtt ttctccggga
    11161 gacggccgcc gcaaaatcac ggcgtcgccg gtcaccacct cacgccgaag atcggttcct
    11221 cctcgacgat gtttgtgatc ctcctgacgg ttgcctccat cgggacatcc tccccgctgt
    11281 tcagggactg tttcagctgc tcaaggccgg cagccaccga atcatccttc gaccggacac
    11341 cgcccaccgt caccctgaac gtcagcgtca ccagccactg ggttgtctcc atctcttttc
    11401 cctcgtaacg tgggcggagt ataccatcgg ggaatataaa gccagcttgc gaccggaaga
    11461 cttcctgcca gcannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggtatgg
    11581 agcaaaggat ggagaaccgt gagatgagaa cggagaggta gtacgacggc ctgagggtat
    11641 ccgaaccgtg acgttctgta aagagttata gagcgttcaa acatgggtga agatacgtgt
    11701 cgcgaactgc ccgatcaacc catccggtac tatctccccc gtatcccggg tgtagtatac
    11761 acatggggcc ataaatatat ttctgggtgg aatgaccttc acaatgtcgc ggtatcgccc
    11821 cgggcccggc ggagagcaac ccgtgcacaa cagatatata caggcaccgg ggactctggg
    11881 gtccagaaga accgcaccgg ggagggggat atgattcagg agcccgcgac tgctcccggc
    11941 aggagcatgg ggaggcgcac gattggctgg atatccggta tgttcatcat actcctggcg
    12001 ctcattccgg cagcggcggc aggtgccccg acggtgatcg tctccaacta caccgtcacc
    12061 cccgccgtcc tgatgcccgg cgacgaaggg acgatcacgg tggtgctcac gagcacggca
    12121 tccatggccc cggggtcgcc gctcgacctc cgggtggata caggaggggt gaacacctcc
    12181 ccgatcaccc cagataacgc ctacatcgag aacgtactct tacgcagcag agagatcgag
    12241 gtcctgtccg ggagtttcca ggacgtcggc gagatcggtc cgggccaatc cctcccgatc
    12301 acgtttcagg tacgcgctcc cggggaggaa ggcatctatt tcccggaggt ctgggtcagc
    12361 gtgcgggatg cgacgggcgt gcgctacccc ataccggtga atgtcggttc tgcctacgcc
    12421 ctggcgaaaa aaccctcgct ccgggtggag aggaccgtcc cggcatccgt cgacccgggc
    12481 gactcattcg gcgtcacgct cacgctctat aacgacgggg aggcgagtgc gaacgatata
    12541 tcggtcggca tcaacgcaag ctccgactcg atcgcttcga cgagcccgga gagttactat
    12601 atcccggtac tcaggccggg agaggaggca gccctgaacc tatcctttgg gaccgatccc
    12661 ggcgcgccgc tcggccttct gccggtcgtc gccaccgtcg actaccggag cgccgatctc
    12721 gcggtgttcc ggcaggtcga gacgatcggt atccccatcg tcggccgcgc cgatatgagc
    12781 gtcgcctcca tcaggaccga acctgcccgg atcaccgccg gggatccggt aggcctgacg
    12841 atccggatag agaacaccgg aacggcggat gcgacgtcgg ttcgggcgac catcggcggc
    12901 ctctcgcttt cgggaacaaa agaagcgttc ctcgggacga tagagcccgg caacgacggt
    12961 cccgccgtat tcaacctgca ggccggcgag gagggggatt acccctatac cctcgccatc
    13021 cagtataccg acgattacgg agcgcacacg accgagcagg ccctgaacct ggttgttgcc
    13081 ggatccgacc gggccccggc cctgatcatc gcggtggtcg ccatagtcgt cgtcatcgca
    13141 gccgcgctct actggtaccg gagacgaaag gaggagtgac cttccccatg ttcaccggtc
    13201 tcagggtctc cgcgttcctt gcctggaaat ccatagaacg cgggagcaag gggacgctcg
    13261 ccctcacgat catgatcatc gccctcatct tcgtgaacct gatcttcctc ccctccatca
    13321 tatccggggt tgtcagggcc ttcgatgacc agtcgatcga cttcgacttc gggaacctgg
    13381 tcatcgaacc ccgggaaggg gaccagtaca tcgacgacgc ggccaacctt cagaggaagg
    13441 tcgatcggat cccggggatc accggcacat cgccccggat agccgccggg gtgacctact
    13501 tctaccgggg ccgcaacgcc gccagcaccc tctacggtat aaagcctgcg gatgagcggg
    13561 cggtgacgaa gatcgccgac cgtatggtcg agggcgagtt tctctcaaac ggggacaccg
    13621 acgcgatcat catgggcacg acccttgccg ggcgggaggg ggaggaggcg ggcggcctcc
    13681 cctccctccg gggggtcgtg gtcggcagtc cggtagaggt ggtctacccg aacggagaag
    13741 cgcgaaccta ccgcgtgaaa gggatctacg agacgcaatc catcagcatc gatgcgtctg
    13801 ccttcgtcac cgcccgggag atgaacggag tgctgggact ccatgatcag gcttccatca
    13861 ttctggtgaa gaccgatgtc aacggcaggg aggcggaata caagacagaa ctcctctcct
    13921 acggtatcca ggaagatatc tggacctacc aggagaagtc gggggggttt gtcaaccagg
    13981 cgatccagag tttcgacatc atcaccgcta tatcggcact cgtcagcctg atcatcgccg
    14041 tcgtcgtcgt cttcatcgtg atcttcatca acaccgtgaa ccggcggcgg cagatcggta
    14101 tactgaaggc gatcggcatc agccagggcg tcatcgtcaa ctcctacatc ctccaggtgc
    14161 tcttcatcac cgccgtcggg acactggccg ggatagctct caccgccggt ttgacgatct
    14221 acctgactgc ctacccgctc gccttccccg gaggaccggt ctcccccgcc gtggaggcgt
    14281 cggccgtcat ccagagcatc gcaagcctct ttgcggtctc ggtggctgcc ggttatatcc
    14341 cggcctggcg ggttgccaac gaggatatcc tcaccgctat cagggggtga accatgatcg
    14401 tcgcagagaa tctcacccgg gtctacgcca tgggcagggt ggaggtccag gccctccgcg
    14461 gcgtctcgct tgaggttggg aaaggcgagt tcgtcggtat catgggagcg agcggcagcg
    14521 gcaaatcgac cctcctccat atcctcgggc tcctcgatcg tcccacctcc ggcagggtca
    14581 cgatcgacgg aaccgatgtc cttgccctct ccgaccaccg gcgaaccctc ttccggttga
    14641 accggcttgg ctacgtcttc caggactacg ccctcatcgg ggagctcacg gcgcttgaga
    14701 acgtctactt aacatcgctc gtcagggggg atccggagga ggagtacctc aggaagagca
    14761 ccgcgatact cgagcgggtg ggtcttgccg gccggacgaa ccatcggcag agcgagctct
    14821 cgggcgggga gcagcagcgg gtggcgatcg cccgggcgct cgtgaacaac ccaagcatcc
    14881 tccttgccga cgaaccctgt gcgaacctcg acagccaggc gtcaaagagc atcctcgacc
    14941 ttttcgcgcg gttaaacgag gacctcgacc agacgatcgt gatggtctcg cacgaggagt
    15001 ggcacaagga gtacttctgc cgggtcgtca ccctgcggga cgggctgatc ggggatacgg
    15061 aggagtgcag gagggagggc ccgcccccgg gccggacggc ctgatacgag cgcccccgca
    15121 catcgcctcg atgcggtgtt cgcgaagcga atgcatccga ctgcaaaggc tgcgtcagcg
    15181 gggttgcagg aagaaaatgc ggcgacaata tttttatatc actcgcgtta taccggtccg
    15241 gccggtgaga ggtatgcaga acgtcaggac gttcaggtgc aaggatctcg gccttgcgtg
    15301 tgaatttgaa gagaaggcgg agggcgagaa cgagctgatg aagcggatcg aggggcacgt
    15361 ccagaccgcc caccagatga atctcgagca cccggaagtg caggagaggg tccggaaggc
    15421 gatgaagtga agggggcatc cctggacccc ctttcgtcgg ttgttcaccg gaggttattt
    15481 tgaggagcag caggctctgg tctgctatat gctaccggga tcgatacact ttatgcgacc
    15541 gggaaggcat tcaacggtgg atgaggctgc acggaggcgc tggcagcacc ccgagtccat
    15601 ccttgagggg atcgggcttg ccgctggcgc caccttcatc gacgtcgggt gcggggatgg
    15661 gttcttcgcg cttcctgcag cccggatagt cgggccgcac ggccgtatct gcgggatcga
    15721 tatcgatgcc ggggcgctcg ataccctcaa aaagagagcc cgcggtgaag ggcttgagaa
    15781 catcgccctc caccggggaa tggccgagga gatcgtcctc tgcgagggct gcgccgacgt
    15841 cgtcttcttc ggcatcgatc tccacgactt caccgatccg gtccgggtac tcttgaacgc
    15901 aaagaggatg gcggcgcccg ggggcgtgat cgtcgatctg gactggaaga aggaacccct
    15961 cccgttcggc ccgccggtcg caaagaagtt caccacggag catgccgcgt cattgatggc
    16021 aaaggccggg cttacggtcg cggcggtggc ggactcgggg ccctaccact acatcatcac
    16081 ggccgtgccg gggcccggcc gggtccggta aaaaagggtt tttgggttac ttcaggtttg
    16141 ctatgccgcc gtagtcgatg gcgttcacga acgcatcgag gtcttgtttg gagccgccgt
    16201 ctacgctgac gtagaccata aaccgttcgt tcacaccgac ccaggtacca taggagtcag
    16261 gcttgctgaa gacttcccag gcggggtgac caccgattgt ctttgacttg tagtagcctt
    16321 cagtcgtctc gatcgaggtg aacgtgttcc acatctgcca ttggcccacg ttgtagtagg
    16381 cggagtcctg gatcacgatt gctgcctggg cgtcgccctt tgtgtaatcc cgggatgacc
    16441 aggtccactg gccgtcttcg accgccatcg tgatgcccac aggctcttct gccgtccagc
    16501 cggcaggtgc ggccggcaag aacgttatca gggtttcaaa cggaactgcg gttacgcttc
    16561 ctctggcggc accggagacg gtggtgccgg cagcaggagc cgtcgtggct gcctccggcg
    16621 tggtcgtatc cgaagaactc gatgtgcagc ccgccgtcat gcagagacag atcccgagca
    16681 ccacgatgag cgccgggaag agcagtttcg aggaggtggt tttcattatc gtgccctcag
    16741 aggatgatag acgggatgga tatatttata cgttgtcatt catcgaaaac agcatctctc
    16801 cccgggccgg aacacggttc cggcttgtcg gccggtcttc tctgatccgc gatccgtctc
    16861 ttccgacgcc tgtacaggct cctcgcgata ggcaaaatgg tttaggaaag tttttattgc
    16921 cgatatacgg acactcattc atgattcagg taaagagaat ccatggtatc gtgattgcag
    16981 gactcctggt agcctgcatg ctcatggccg gttgcacgtc gcaaccggag aacacgacgg
    17041 tcacaccctc tccggcggca accacggcgg tcacaccctc tccggcggaa cggttcgcct
    17101 tcaacgagac cgacgacaac aaggccgtca ccctcccggc cgggagcgag atcgtcatca
    17161 gcctcgacga gaacccgacg accggatatt catggaacgt cacctcgtcc cgggggctct
    17221 cgtacgtgaa cgacacattc atcccgccaa agacggacct tgtgggtgcc gggggcgtgc
    17281 acgagtggca gtaccgcgct gccgagaagg gatccgctga gttctccgcg atctatatgc
    17341 agccctggat gaacgtgacc ggaagcgaga ccacgttctc gatgacgttc accatcgagt
    17401 gatcccccct gccctcttcc gcaaccgtgc gggggagagg catccccttt tttgacatcc
    17461 cgatctctgc cctgccgcca tccggtcatc cccccgggtg cccgtcgtca ccactcgttc
    17521 ccgaaccatc gcgagacctc cgcccccgcc cacggccccc cgtgcgccgc gtagatccgt
    17581 tccggtccga gggcgagaag ggcccggata ctccgtcgcg ctgccgcagg atcgccggta
    17641 cggaagggca gacggggcct cccgcccggg aggagggcgg agaggagatc gccgacgatg
    17701 gcggcgcctc cggcgatgaa gaccgagacc gagcccgggg tatggcccgg cgtggggatg
    17761 accctgcccc ggatcccgaa gaccgcgaga tcggcggcgc cctcgatgac gatgtcgggc
    17821 tcgatgcttc ctgtcttccc cgccataaac ccgatctggt gcgggacgat ccgggcgaga
    17881 gagaaccgtc cggcaacccc gcagcgcacg gcgccggcgt cggccgcatg gacggcgatt
    17941 ggcgcgccgg tccggtcccg gagggcatca gcgctcccgt aatggtccgg gtggccgtgg
    18001 gtgaccagga tcagcgagag atcatcgggg cggatgcccg cacgcctcat cgcggccagg
    18061 atggccgctt catccccggg attaccggta tcgatcagga gcaccccccg atccctgacg
    18121 atgaacgctg tggtcgggcc gagcggcacg gggatgaccg ccgggaggga acaggcgttg
    18181 atgcgccctc gcccgggcac cggagcccgg acagcctgca tacccggatc tacggtcgcc
    18241 atgacatccc cccaccgtga gatacatcct ccccacatca atcattcggt cggggtggga
    18301 agcggaagcg gatggtcggg ctgtcaccgg agatctgcgg cgcgccggca gaagcaccgg
    18361 ggagcccgca gaagatgaac ggggcgggag gcggtccgtt cccgggaccc gggccgcaaa
    18421 aaaaattgga gggtattcga ttgaaagtta cgcgagatag tcttccggcc gggggagctg
    18481 ctccttcagg cccttcctcc gccggatgcc cttgaccacg tcgtccacga tgccggaggg
    18541 gacgagttcg aacccggcga actcggtact ccacatcgcg cggccttcgg tggccgaccg
    18601 gacgtcgccc gcgaacccga agagctcggc gaccggcgcc ctgccgacga tcgtcatcgt
    18661 gtcgccttcg ctcagcatat cgaagacctg gccgcgccgg ccctggatct gcgaggtcgc
    18721 cgcacccatc tgatcgctcg ggaccgtgat ctggagcttc tggataggct cgaggagcga
    18781 atcgccgccc atcagcaggc ccgccttgac ggcgctcctg accgccggga tgacctgggc
    18841 gggaccgcgg tggatggcat cctcgtggag tttcacatcc accaggcgga tcttcaggtt
    18901 ctggaccagc tcgtcggcga gcggacctcc gtggagcgcc tcacgccagc cctcgaggac
    18961 cagttccatc gtctcgttga ggtactggat acccttcgtc atgtcgatga acatgttggt
    19021 gccctcgatc gccttgacgt tcttggcctc gtccttatcc ataccggcgg cgatgagggc
    19081 gtcacgccgc tccaccgcct gctgggtcat cgagacgtcg ccctgctgga tcagcttcac
    19141 gacctcgggg ttcatcggtt cgagttcaag gtagaaccgg ttgtgccggt tcggcgactt
    19201 cccttcgacc gggcccgccc tgcccgtgat cgtctcgcgg tagacgacga tcggcggaga
    19261 ggtgatgata tcgacgccct tgtcgcgctt gatacggccg gtgatgatct cgaggtggag
    19321 ctcgcccatg ccgctgatca ggtgttcgcc ggtctcctcg ttgatcgaga cctgcaccgt
    19381 cgggtcttcc tttgcaacct ggcggaggac ctcgacgagc ttcgggagat ccttcatgtt
    19441 cttcgcctca acggcgacgg tcatgaccgg ctcggagtag tgtttcagcg actcgaaggg
    19501 ggtcatgtcg acgagcgtcg tgacggtcga gccgacgatg gcatctttta agcccgtgac
    19561 ggcggcgatg ttgccggcag ccagttcttc cacctcgatc cgctcggcgc ccatgaagat
    19621 gccgacctgg gcgaggcggt ttgcgcgttt cgccgcaccc atgatgtagc actcggtacc
    19681 ccggcggagc gtccccgaga agaggcggcc ggtggcgacc tcgcctgcgt gcgggtcgaa
    19741 ggtgatatcg gtgaccatca tgcagacggg accgttcgga tcgcaggtga gcatcgcctt
    19801 gccctcggag ctcgtgtagt cgccgtgcca gatgatgtgg atacgccggt cctgggcctg
    19861 gagggggttc gggaggtgct tgaccaccat atcgaggagc acggcgtgca gggggcttga
    19921 ctttgccagc cacttcatgt cgccggtgtt gcacttctcg tagacatctc tgaacgagac
    19981 gccgctcttc ttcatgtagg ggacggagac tgcccagttg tagagcgcgg agccgaaggc
    20041 gacggtgcct ttcgcggcat cgagcttcca gccctcgttg tacatcttct cgttcatgcc
    20101 cttgatcagc ttgttgacct tatcgatcac ccgtgcaagg cggatctgca tctcctgctc
    20161 gtccaccttg agctcgttga tcagccggtc caccttgttg atgaagagca ccgggcggac
    20221 gccttccttc aaggcctggc gaagcaccgt ctccgtctgg ggcatggtgc cttcgaccgc
    20281 atccacgacg acgacggcgc catccaccgc gcgcatggcg cgggtcacgt cgccgccgaa
    20341 gtccacgtgg ccgggggtat cgatcatgtt gatcaggtac tcctcgccgg cgtactcgtg
    20401 gaccatcgag acgttgcttg cgtcgatggt gatgccgcgt gcctgttcct cttcgtcaga
    20461 gtccatgaag agctgcctgc cggcgagctc ctcgctgatc atgcctgctc ctgcgagcag
    20521 gttgtctgag agggttgtct ttccgtgatc gatgtgggcg acgataccga tgttccggat
    20581 gtgttccggc ttgtccatca ggtccgtcac ccgctctacc atcttctttc gtctggtcat
    20641 agaacaccaa aaaagtaaag gggatgttat cgggcagcct ttgcgatacg ctctctctct
    20701 tctctcttcg agacggcgta ggagcgggtg tcgccgtttg cggccgcgat cagttcctcg
    20761 gcgagcgcct cgctcacggt cttcttcttc ttgtggctcg cctgccggac gccgtcggtg
    20821 atgaaccgga gagcggtgtc gacacggcgc tggggtgcgg tgtcgaccga tttcgggacg
    20881 ttgataccgc cgtacttcag ccggacggtc tcttcgcggg gaccggcgtt ctcgacggca
    20941 tccaccagca cctggaccgg gttcttcttt gtctttttat tgacgatctc gagagcatcc
    21001 ttgacgatgc ggatggcgag ctccttcttg ccggtgttgt gctcggtctg catcatcttg
    21061 ttgatcaggc gctcgacgat cagcatattg ctcttgttga actgctggcc ggcgagcttg
    21121 ccgcaggagt gcggcacgat catcgcgtgc aggttcacgt aacgggcaag gctcggatct
    21181 ttgatctcca cctcgctcat gtcccagcgg ttgaagagga gtttctggtc ctggatgcct
    21241 gcttctgctg cttcttccat actccttacc tccgcggctt ctccttgcgc ccgatgacca
    21301 tctcgctgag actgacgttg ttgaccttgg tcacgacgaa ccggacgccc gggatatcgc
    21361 ccatcgacct gcccagcctg ccgccgatac cctcgatctc gacctcgtcg tgctcgtcga
    21421 tgaagttgat cgcgccgtcg ccgacggcga acgcggtcac ctggcggccg ttcttgatca
    21481 gctgtacgcg gacgcactta cggatcgccg agttaggctg ttttgcctcg acacccacct
    21541 tctcaagaac gatcccgcgt gcctgcggcg ctccctcaag ggggtcggat ttcacatcaa
    21601 gtccaagctg gcgccgggcg tagacaggat catgccacct gtctcttttc gcgtcgcgct
    21661 tcagttttcg ggctgcgaat ttaccatttc ccattgaata ccctcgtatc ggttgaattt
    21721 gtggatagga gcaactcccg gtgtgctata aagcaacgcg cgttacggat ttatatattg
    21781 tggaacccat ccgcgcgtcg atctttcgcc ctagttattt gatcgggagc tgatataata
    21841 ttatatcctg gtgagcaacc ttcatggatg acaacccgga tatacaaaga ggtttttttc
    21901 gaggcgaccc atcgcctgat ccactatcgg gggaagtgct ttcgcctgca cggtcaccag
    21961 tggcgggtgg aggtctggat cgaggggacg gcggacgagc gcaccgggat cgtccttgac
    22021 tacaactgca taaaggaggt cgtcgggcgg ttcgaccacc aggtgatcct gaacgtcggc
    22081 gaccccatgg cggcgtgcat cgaggcgttc cacccggtcg tcaccacgcc cggcgacccc
    22141 acgagcgaac tcctcgccgg cctcatcgcg gatatgatcg atgccgaggc cgcgcggcag
    22201 gggcttgatg cccgcgtgaa gaagatccgt gtctgggagt cgacgtcctg ttacgcagag
    22261 cgcacgtatg atcgtcagtg agatcttccg gagcctccag ggagagggca ggaaccaggg
    22321 gcggccctgc acgttcatcc ggctcgccgg gtgcaacctc cggtgcgcct ggtgcgacac
    22381 ctcctacgcg tgggaaggcg gaatgaggat gagcgttgcg gaggtcctcg accgggtctg
    22441 gctcctgaac gggaagcaga tctgcatcac cggcggggaa ccgctcctgc agcgggacga
    22501 ggtcgccggg ctcctcgaaa agttcgaccg ccacggttat acggtcgaga tcgagacgaa
    22561 cggaactcag gacttccggg atatgcaggc gtttgcctcc atctgcatgg acgtgaaatg
    22621 cccctcatcg ggggagcaaa gcgatctctc tctcctcccc ttcatcggcc ggcgcgactg
    22681 cgtcaagttc gtggtggcgg acgagaacga cttcctctac gcgcgagcgg tgatggcccg
    22741 ttacgatatc cggggcgagg tcatcataac accggtggaa ggatccgact accgtgccat
    22801 cgcggattat atcgtcgaag agaacctccc ggtgaggttt caactacagc tccacaagat
    22861 cctggggatg aaatgatgag agctgtctgc cttctttcgg gcggtatgga ctctgccacg
    22921 ctcgcctacg ccgcaaagga tatgggctac gacatcatcg cgctccactt cgcctatggc
    22981 cagaggacgg agaagcggga gcgtgcatgc gcccggacca tcgcccgtat gcttgatgcg
    23041 gcggagttcg tcgagatcgg cctcgagcac ttcaaaaaga tcggatcctc gagcctgacg
    23101 gatgagagca tcccgatcga cgagtatacc gagaaggggg tgccggcaac ctacgttccc
    23161 ttccgaaacg caaacctcct tgccatcgcg accagcctcg cggaggcccg gcacgcggag
    23221 gcgatcttca tcggcgtcca gaccggcgac taccccgggt acccggactg ccggccggag
    23281 ttcatcgagg cgttccagca cgcgatcgat atcggcacgg cggcggatcc cccgatcacc
    23341 ctgatgacgc ccttcgtccg tatgacgaag gcggatatcc tccgcgtggg gcttcgcctc
    23401 ggcgtcccct acgaggagac ctggtcctgc taccagaaca acgaccgggc gtgcggggtc
    23461 tgctcctcgt gccactaccg gcaaactgca ttccgcgaac tcggactttg cgatccgatc
    23521 gcgtacgagg actgaccatg gagatctacc gcgggaagcg actcaccgtc gagaagagag
    23581 agatcaccct cccgaatgga aagcgaaagg agcgggtggt cgtccatccc ggcggggcgg
    23641 tcgcgatcct cccggtcgag ggagacgact gttacctcat ccggcagtac cgctacgccg
    23701 tcgacgatta catcttcgaa gcgccggcgg gcaccatcga cgagggcgag gagccgcacg
    23761 agaccgcccg ccgggaactg atcgaggaga ccgggatgaa ggcggaggtc ttcaccccga
    23821 aaggctggat ctacccttcg cccggttaca ccgacgagcg gatatggctc tacatcgccg
    23881 aaaggctctc gccctgccat gactacgata tggacgagga cgaggtgatc gaggtcgtcc
    23941 gcaccccgct cagggatctt gcggccatga tcgccgatgg gcgaatagtc gacgcaaaga
    24001 cgatctgtct catctgccgg tgcttcttgt gagaagagcg cgaggcgccc ggcccggggc
    24061 gggcgcggga gggggatggc aaatgtgcgc agatttaagc catctccgga gaaacacata
    24121 gtagcttcga ccagtgaata ccattcagcg agcctggtga atacccgatg gaagagaccc
    24181 acgagatgga taccgcgcga aagcgctacg aatttaaaaa gatgcttgag aggcttgccg
    24241 agaaggaggg gagcggcacc gagctgatca cgctctatat acctccggat aagcagatat
    24301 acgacgtgac cgcccagctc cgcgacgagt tcggacagtg tgcgaacatc aagagcaaac
    24361 agacccggac aaacgtccag agcgccatat cctccatcct ctcccgcctg aagtactaca
    24421 agcgcccgcc ggagaacggt atggcggtct tctgcggcac gatcaacatc ggcggcgacc
    24481 gcaccgacct tgagtgcgag atcatcgagc cgcctgaacc gctcaacacc tacctgtacc
    24541 ggtgcagttc ctcgttcgag ctcgagcccc tgcagcagat gctcggcgag aaggaggtct
    24601 acggcctgat cgtcatcgac cggcgggagg cctacatcgg tttcctccgg ggcaaccgga
    24661 tcgaggcggt gcgcggcgcc acatcgacgg tccccggcaa gcagcggaag ggcggtcagt
    24721 cgagcgtccg gttccagcgg ttgcggttga tcgccatcaa cgagttctac aagaaggtcg
    24781 gcgaccttgc aaacgatatc ttcctcgccg agaaggactt cttcgagcgg ttcaaaggcg
    24841 tcctgatcgg cggcccgacc cccacgaagg aggagttcga ggcgggcggc taccttcacc
    24901 acgagctcca gaagcggatc atcgggctct tcgatgtggc ctacacaaac gagtccggcc
    24961 ttgccgagct cgtcgagaac gcatcggatg cgctgaaggg cctcgatatc atcaaggaga
    25021 agaacatcat gaaccggttc ctccaggaac tggtcaaaga cgacgggccg gccgcctacg
    25081 gcgaggagag cgtccgaaag aacctggaac tcggctccgt cgataccctc ctcctctccg
    25141 ataagctccg gagggcccgg ctgaaacttg cctgcccgaa cagcgactac accgaagaga
    25201 gaacgatcag catcgaaccg ggcaaacata tcaaggacat cgacttcggc acctgcccga
    25261 atgacggtgc ctctctctac gtcgcggagg aggtcgacat catcgacgag ctgacggcgc
    25321 ttgcggacca gagcagcacg acggttcgga tcatatcgga cgacttcgag gaaggctcga
    25381 tgctctacaa cgcattcggc gggatcgcgg caatacttcg ttataggacc ggatactgat
    25441 gtttcaggag aagtaccacc aggttgagcg catgctccgg gcatgcaccg gcgaagagga
    25501 tgtcctcctc acgatagggg gggatcacgc cgatatcgcc tcgaccgtag cgtttaaact
    25561 tgcgaagaag gagaggcggg cgccccagaa gatcgctgcc gatatcgcgg caaggctctc
    25621 ggcggacccc gatctcgggg atatccgcgt cggggcggtg ggcccctacg tcaacttccg
    25681 gttcggaccc tcgcttctct cggaggcggt cagggcggcg acaaaacccg gttacgggaa
    25741 cctcccccgc cgcaccgaac gggtggtgct cgagcacacg agcgcaaacc cgaacggtcc
    25801 gctccacgtc ggtcacatca ggaacaccgt catcggggat accctggtcc gggcgttccg
    25861 gaaggccgga tacccgctcg aggcgcagta ctacttaaac gatatgggcc gccagatcgc
    25921 catcgtctcc tggggattcg accacctcgg ccttgcccgc ggggagggtg agaaggcgga
    25981 ccattacgtc gcccgggtct acgtcgcggc gaaccgggcg atccgggaga acccggagat
    26041 cacggaggag atcgaccgcc tcatgcagca ggtggagcgc ggcgatgcag agaccgtcac
    26101 aaagttccgg gcggccgtct ccctctgcgc ggaaggcatc aaagcgaccc tcgccgccct
    26161 gaacgcccgc cacgaccggt tcgtctggga gagcgatttc gtccggatag gcgatgtgga
    26221 ccggatcatc gagcgggtca ggcgcctgcc gcaggcccac gaggacgaga tcctctgggt
    26281 cgacctctcg gagcagggtt tcgagaagaa gtacatcctc cggaggagcg acggcacgtc
    26341 ggtctacagc acgcgcgacc ttgcctacca cacctggaag gcgcgcaact tcgaccggat
    26401 gatcgatatt ctcggagcgg accacaaact gatcggcgcc cagctccggg ccaccctcga
    26461 gcttctcggc gaacagccgc ccgatatcgt ctacttcgag ttcgtctctc tccccgaggg
    26521 ctcgatgagc acccgggcgg gcaagttcgt ctcggcggac gaactgatcg aggaggtgac
    26581 cgcgaaggca tacgacgagg tgaccgtccg tcggccggag ctccccgaag gggagcggcg
    26641 ggccatcgcg ggatcggtcg ccgtcgccgc cattcgctac gatatcgtga aggtctcccc
    26701 cgagaagagc acggtctttg actggcggga ggcgctgaac ttcgagcggc agagcggccc
    26761 ttacatccag tacgcccacg cccgcgcctg cagcatactc gataaggcag gagcggtctc
    26821 gatgccggca taccactacg agacggagca cgaggtcgcg cttgcccggc accttgcccg
    26881 gttccccgtc gtcgtcgagc agaccgtgac ggagcttcgc ccgcacctgc tcgcctcgta
    26941 tgcccgcgag ctcgccgacc tcttcaacgc cttctaccac tacgacccgg tgctgaagag
    27001 tgagggcgag gcccggaaca gccgccttgc cctcgtggat gcggtcagga acacccttca
    27061 ggagtcgctt gcgacactcg gtatcgatgc actcagaagc atgtaaggcc ctccggaagc
    27121 agggctacca gttcgtcagc cctgcctcgt cggcggccgt aaagccctgc atgtggaaca
    27181 agcgggcact ccggggcgga gctatgtgct acaaggcaca gttctacggc atcgagagcc
    27241 accgctgcgt ccagatgacg ccgacgctac gctgcaacca gcagtgcctc ttctgctggc
    27301 ggtcgttcga gcacgaggtc gtggaggagg aggagtgtcc gccggagacg atcatagaga
    27361 gcctcccgcg tctccagaag aaggcgctcg cgggctacaa ggtctcgcct tacgtcgcgc
    27421 cggaacggtt cgcggaggcc ctccgcccca cgatggtcgc ggtatccctc tcgggggaac
    27481 cgacctgcta ctcgcaactc ccaaacctca tcgacggcct caacgccggc gggtacacga
    27541 cgttcctcgt ctcgaacggc acccggcccg acgtcctcgc gcgatgccgg ccctaccagg
    27601 cctacgtctc tcttgatgct cccgaccggg agacctacct cggcctctgc cggccgcggg
    27661 aagactactg ggactgcatc caggagagcc ttgccgggct tgccggccgc cgttccgcta
    27721 tccggaccac cgtggtgcgc ggctacaacg acttcgcgcc tgaagggtat gcggcgatct
    27781 accaggactc cggcgcccga ttcgtcgaag tgaagggtta tatgtacctg ggatacagta
    27841 gaaaacgatt gcagagagac cagatgcccg aacatagcga tgtgcgggcg tttgccgaga
    27901 agattgcaga acactgcgac tataccgtaa aggacgagag cccggcaagc cgggtcgtct
    27961 gcctggagcg gaagatatga gattcgatgt ggaagagttc aaaaagagag cgagagagga
    28021 cttcgagcac gcctggcacg aagggccgtc ggtgctgacc ccgccgggga tctcgggcca
    28081 atatccccgt ctgaagtaca cccgggccac ggcgcacccg atatttgaga tcacgcagag
    28141 gctccgcgag acctacctct cgatggggtt tgacgaggcg atgaaccccc tgatcgtcga
    28201 ggaagcggat atctaccggc agttcgggcc cgaagcgatg gccgtcctcg accgggtctt
    28261 ctacctcggc ggcctgcccc gcccgaacgt cgggattgca agaaagcaga tcgaggggat
    28321 cgaggcgatc ctcggccgca cgatcacgcc cccggtcgag gagagactcc gcgaaaccct
    28381 tcacggctac aagaagggga cgatcgacgg cgacgacctg acgcacgagc ttgcggccgt
    28441 cctcgaggcc gacgatgccg ccgtggtgca tatcctcgac gcggtcttcc ccgagttccg
    28501 cgcgctcgcc cccgagtcgt cgcgcaacac cctccggagc cacatgacga gcggctggtt
    28561 cctgacgctt tcggccatct gggagaagcg acatctgcct atccggctct tctcggtgga
    28621 ccgctgtttc cggcgggagc aggaggaggg gccgacccgc ctgatggcct accactcggc
    28681 ctcctgcgtc gtcgcggggg aggacgtcac cctcgaagag ggaaaagccg tcagcgaggc
    28741 cctcctctcg gccttcgggt tcaccgagtt caggttccag cccgacgaga agcggtcgaa
    28801 gtactacatg cccgggacac agaccgaggt ctacgcccgc cacccggtcc tcgactgggt
    28861 cgaggtcgcc acgttcggca tctactcgcc ctcggcgctc gcggagtacg ggatcggcgt
    28921 gccggtgatg aacctcggtc tcggggtgga gcggctcgcc atgatcgcct accggtctaa
    28981 cgatatccgc gagctcacct acccgcagtt cttcccgcag accctctccg accgcgatat
    29041 cgcgggcgcc gtccatctcc gcacggaacc ggcgtcgacg accgggaagc ggatggcgga
    29101 ggcgatccgt gctacggccg ctgagcacgc aaccgccccg ggcccgtgcg cgttcaccgc
    29161 gtggaagggc gagattgcgg ggagagaggt cgaggtgatc gtcgaggagc ccgagtccaa
    29221 cacgaaactc tgcgggcccg cgtgcgcaaa cgaggtctat gttcacgacg gttcggtcct
    29281 cggtgtccct gataccgaga agtgggcatc cgtccggcgc gaaggggtct ctaccggcat
    29341 cacctacctc gatgccgtgg caggccttgc cgccgcccgg atcgaggagg ctgcacggtg
    29401 cggggagggt accacagtcc aggtgaagat gtcgaagctt ccttccgacg tcaacctccg
    29461 tatcgaggag tacgcgatgc ggtatatcac cgaccacaac aagaaggtgg acctcagggg
    29521 accggtcttc ctgacggtgc gatccgtcat accggagcag tcgacccgat aagactcttc
    29581 tccctccccg gtttctctct tttccgggtc accccctccc tgccgcactc gatacacctc
    29641 cggagcaggg gcaggacgtc ccgttgcgag accaggttga aacgtgccct gctcggggcg
    29701 agtccgacct tgatcgagta ggcgtcgggc ggcatcgccg cgaagagatc ttcatcggtc
    29761 ctgtcgtccc cgatggcgag gatgaagtcc caggcgtgct gcgagaccca attcaacgcc
    29821 gccctccctt tatcgacgac ggtgttcttg atctcgatga ccttgttgcc ctccatgacg
    29881 ccgacgttga ggttcgacgt caggtgcagg aggtcgtctt tga
//
LOCUS       DAOSII010000007        27995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_1893_length_27995_cov_56.565068, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000007 DAOSII010000000
VERSION     DAOSII010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 27995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27995
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1893_length_27995_cov_56.565068"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..3107)
                     /locus_tag="PLV90_01155"
     CDS             complement(108..3107)
                     /locus_tag="PLV90_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HOF95750.1"
                     /translation="MMRQHFRRTLVMGLCLVVMVFSISIGTVSAAGPTADFTGNVTTG
                     TAPLTVKFTDESTGNPDGWAWYFGDETYEGEWERVNESAWPKRYDHSSVVMPDGSIVL
                     MGGYDVDSDAYNDTWRSDDYGTTWECVNESSGWMERLRHSSVVMPDENIILMGGSGGP
                     GTLKDIWRSDDGGKTWTCVNPSAEWTKRAGHTSVVMPDGSIVIMGGGGVGYNDTWRST
                     DGGETWTQLPDAGWTGRYYHTGVVMPDGGIVIMGGTYWDGNSHHFLNDVWQSDDYGGN
                     WTQLPDAEWSKRDGHSSVVMPDGSIVIMGGGGVGYNDTWRSTDGGETWTQLPDAGWTG
                     RYYHTGVVMPDGSIIIMGGWDEGMGVSLNDVWRLKTAGNQTRNPTHTYTEPGTYTVTL
                     QAYNDDGHNVTQKVAYITVTPPPPEAGFSATPLSGPAPLTVKFTDESTGDPDGWTWYF
                     GDETYEGPWERVNESAWPKRLLHDSVALPDGSIVIMGGIASGSTRVNDTWRSTDRGAT
                     WTQLPDANWTVRNGHAAVALPDGSIVLMGGVADGSKCQNDTWRSDDYGASWTLMNASS
                     GWMGREFHSSVALPDENIIIMGGNNGDKYLNDIWRSADRGATWTQVTEHAEWTARYGN
                     AAVALPDGSIVLTGGEESYEYLNDTWRSTDGGATWTQVTEHAEWTGRRYHTAVAMPDG
                     STVLMGGNGGEFQDDVWRSTDGGATWTLVNASAWPARSMFASVVMPDGSIILMGGFNN
                     GYRNDVWRLQPAGSTAENPTHTYTEPGTYTVTLQAYNDFGFDSLRKSITVTSPSSSGG
                     GGGGGGGSTVGPGGGYNVGGDSAVSTVTATGTGLTMLIVTGWKQSSPAPGIPPAPGSP
                     YQYIELTPARFEEITGANITFSIPTAWLAEQGFAPDEIVLYRYNGTAWEPLPTWVVDD
                     AGATVTFGATSPGFSLFAISGVRDESPVVTPTAAEATQTTAQLTATETVAVPVDEAAP
                     DFPLRTIALIAGGVLVLAGAGYFTRRW"
     gene            complement(3316..4566)
                     /gene="hisD"
                     /locus_tag="PLV90_01160"
     CDS             complement(3316..4566)
                     /gene="hisD"
                     /locus_tag="PLV90_01160"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOF95751.1"
                     /translation="MWQALEIETWIAGRRSEIDRVRGPVSEIIARVRSEGDAALIDLT
                     KRFDGIDLDEIAVSDEEREAAYDRVDAQLIESLAEAEARISRFHELQRGRDLWLQEME
                     PGITLGVRTTPLSRIGAYVPGGRAAYPSTALMCTVPAKVAGVREICCCTPPPTNPITL
                     VALDIAGVDEIYRVGGAQAIAAMALGTETIPRVEKIVGPGNVYVTAAKMLLRDEAEID
                     FPAGPSEIAILADGTANPTFVAADILAQAEHDPNAACVLVTTDAVLADRVGAEVKRMA
                     AGAVRREIIDRALSHSGYIVAADIDAAVETVDAVAPEHLSIQVADPLAVLTRVRNAGS
                     IFVGPYAAVACGDYASGTNHVLPTAGYARLYSGLDVNHFCRRATVQVITREGLEAIGD
                     VVETIADAEGLHAHAESVRVRRRG"
     gene            4730..5665
                     /locus_tag="PLV90_01165"
     CDS             4730..5665
                     /locus_tag="PLV90_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOF95752.1"
                     /translation="MRSRTWMPILGIMVVLLAACAVSVTAQQAANPGVLRIATTTSLY
                     DTGLLDNLEDMYENVSGVDVQITAQGTGQALDTARRGDVDLVLVHSPGLEQEFIDQGY
                     GINPHCFAYNNFLIVGPDSDPAKIKNMTPEEAFKTIAIAGMNDTPGVAFVSRGDNSGT
                     HNREQQIWKAAGYNYTTDIQDSGDWYVETGSGMGETLTIANQKEAYTLSDIGTYLAFK
                     SQLNLVQLVTEGGELMNRYSAIAVNPEKNPNVNIDEANRFIDWITSNETKEFIGNFGV
                     AEFGQPLFVPLYPPECSQPPFNCTTCSSGEVNATA"
     gene            5881..6855
                     /locus_tag="PLV90_01170"
     CDS             5881..6855
                     /locus_tag="PLV90_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOF95753.1"
                     /translation="MKMNVAILSAGTIAILFILALLAGCTGTAPGENVTPNVTTTAPA
                     AGANVLKIATTTSLENTGLLAELERVYERETGMDLQFIAQGTGQALDTARRGDVDLVL
                     VHAPALEKEFEDEGYGIDPRCIAYNYFIIVGPEDDPAKIRNMSAVEAFRTIAIAGMND
                     TPGVAFVSRGDNSGTHAQEKALWKEAGYNYTTEILDSGAWYVDAGKGMGDTLILADEK
                     KAYTLSDEGTYLSFANRLALVPIISESTELMNRYSAIAVNPAKYPNTNARGAADFINW
                     LISDEGKQLIGDFGKVEFGKPLFVPLYAPECTGPPFNCTCAEPVPTPA"
     gene            6911..7606
                     /locus_tag="PLV90_01175"
     CDS             6911..7606
                     /locus_tag="PLV90_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF95754.1"
                     /translation="MVNGNPIVEGFIEAIRLIVSLDPQVVEITIRSLYVSLTATFFAA
                     LIALPLGALIYFYEFRGKHAVVSTLQTLYALPTVIVGLVMFLLLSNIGPFGFLRLLYT
                     PGGMIVTQTVLIIPLLMGLTVSALSGIDRDKRYTITALGASRLQTIVTIIVESRFAVM
                     SSVLLGFGRAISEVGTVMIIGGNIRGATRVLTTAIALNTSMANYSVSIALGIILLAVA
                     LGVNIALSLVQRR"
     gene            7611..8696
                     /locus_tag="PLV90_01180"
     CDS             7611..8696
                     /locus_tag="PLV90_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF95755.1"
                     /translation="MVIIEARDIRKVYGNLEVLHDVDLSVEEGEILGLIGPSGSGKST
                     LLRILDLIEPPSGGELSVFGTDTVAERSRWLDLRRRMGMLFQRPIVFNASVYDNVAMG
                     LRYRGVSGSDIDRKVKEALDAVGLSRYIKSRATDLSGGEQQRVALSRVLVTDPEILFL
                     DEPTANLDPGSTATIERIVADLNREAGMTVLISTHDLAQGQRLAHRIAVMIEGTVAQA
                     GSPHAVFHEPGSRKIAGFVGVQNIIPGRIVSRQGGLTTVAAKDRRIISATPSPADEVF
                     VVIRGEDISLHRREPGYEEAENLFPARVTAIESMAPFVNVTVDCGCDLVVLMPARRAE
                     ALEIHVGMEVWVSLPAKVVHLVPQNGP"
     gene            8819..9910
                     /gene="wtpA"
                     /locus_tag="PLV90_01185"
     CDS             8819..9910
                     /gene="wtpA"
                     /locus_tag="PLV90_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tungstate ABC transporter substrate-binding
                     protein WtpA"
                     /protein_id="HOF95756.1"
                     /translation="MKSIRMVAFIALIVAAAVLICGCTGTTNTDSTPGTGAQTTTPAS
                     TAAPTENIQVKIFHAGSLTGPFEKVKAEFEAKYPGTTVLLEPAGSIDCVKKITENGKP
                     ADVLASADYYLIPEMMIPDHADWYLTFAKNLMVLTYTNESKYASEITADNWYEILGRD
                     GVRWGFSDPNSDPCGYRTPMVIQLAESHYKNDQIFENLVSKHSNITVTEENGISTIHA
                     GDPKPDGAVLTIRPKSVELVQMVQAGGLDYAWEYRSVAVQNDLRYIELPEAIDLSSID
                     FEDTYATVQIETKKGDSVAIFTGSPIIFGITVPKIAEHPDLGVAFIEMLIDTTGQEIL
                     IADGQPPIVPADGYGNVPDVLKSLVAMRS"
     gene            9977..10867
                     /locus_tag="PLV90_01190"
     CDS             9977..10867
                     /locus_tag="PLV90_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF95757.1"
                     /translation="MDMKTQTRSTDPLPADGEPVAATRWKRERERYTDWCTLGFCILG
                     GLLVGITVLALANITMAELADPAHLIKIATSSTVIESILLTFGAGASAVLLLIVFGTP
                     LAYILARSRPSRFKGVVESIIDIPLILPHTVAGLLVYLLFMRRGWLGAPLSDIGLAFE
                     DALPGTIVAMLFVASPFYINSMREGFEKVPVHLENVARTLGAGTFTMFRTVSLPLSLR
                     HMYSGAILAWGRAIGEFAGVIMIAYYPFIISTLIYYSFTTDGIRASRSIAFTVILVSF
                     GVFYLLRRMTRHLGRYDDRV"
     gene            10854..11921
                     /locus_tag="PLV90_01195"
     CDS             10854..11921
                     /locus_tag="PLV90_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF95758.1"
                     /translation="MIEFDRVSLALGSFRLKEISLTIGRGDYYFIVGPSGAGKTVLLE
                     TIAGLHRPESGRVLLRDEEITTLPPEKRRVALVYQDYSLFPNMRVIDNVSYGLRVQGM
                     RKKEARDEVVPLLERFGIAHLADRYPGTLSGGEQQRVSLARAIAVKPDILLLDEPLSA
                     LDPITQERFIGDLRRLHREDGLTIVQVSHSRREAHLLATRMAVIIDGYLVDEGKAGVV
                     LNTPRSRDVATFVGIENILDGTVTANEDGHATVDAGGRTFEAMTDAAPGEEVSLCIRA
                     DDIILAVDGDRHSSARNTMTGTVVSVVENGPVAEVKVDAGVVLTAVLTRRSVRDLLLV
                     AGTAVTLSIKATAIHVIRASS"
     gene            complement(11902..12156)
                     /locus_tag="PLV90_01200"
     CDS             complement(11902..12156)
                     /locus_tag="PLV90_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HOF95759.1"
                     /translation="MDAITALGLIAGSLTTLSFAPQVVRAWRTRSTADLSLATLIVFL
                     AGLLLWLAYGVVRGDPAIVAANAVTAVFVGLILSIKTKHG"
     gene            complement(12214..13359)
                     /locus_tag="PLV90_01205"
     CDS             complement(12214..13359)
                     /locus_tag="PLV90_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HOF95760.1"
                     /translation="MMEKTRSTGNGIRFTLEEIAGAVGDFGTIFPILLGVAIVSPDVN
                     VSHFFFFLAAWFIIAGLYYRLPIPIEPMKAIGAVVIAGGLSSGEIVASGLIVGALFLL
                     LGLVGGMTWLGDRIPKSVIRGVQAGLALILLRTSLGYIVDDVLFAIVSIAIIVVFFIA
                     SARTRIPDISALLVLLIGLAVGIATQGMPPFRLIPLPPLIVPSLPDFVAGIWDLALPQ
                     IPLTIANAILATSLLTYDLFPKKGVDPDRLSRTIGAMNLISTPLGGFPMCHGAGGLAA
                     MYRFGARTGGANIVAGLFILVFAVVFAPPEVLTLIPFGVFGALLVFVAIELGKHGIKT
                     ESYLVTGVIAVLALALGLTIAFIVGMALAYALEWWKKEQGAGQKPGA"
     gene            complement(13418..14350)
                     /locus_tag="PLV90_01210"
     CDS             complement(13418..14350)
                     /locus_tag="PLV90_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOF95761.1"
                     /translation="MQESVPVLIGGVPLDAFLAFIFAFIVSLFLGNLAYMLLRRLLDG
                     RASRGKVKWAAAILQYGITVGGVYASARYFLDFELTAFFASLGILGIAVAFSSRQIIQ
                     NAVAGVLITINRPIQLEEWVVVSGRPETGLCRVQDISFTTTILQALDGGLVLTPNSSI
                     ITSKVINYSRAGLLEVSVPLAVPVTTEIARVQEIAVAAARDHPLILPHVEPAERSAVQ
                     NLFDLPYIGRLSSDLPRPDLRLFEPKVQVASIKEEWVRLTVRIWIRQIPGKDRIVSEY
                     LDAVIGHLSAEGLLEAKKPEDDPAPGFPGTAEPA"
     gene            complement(14390..15274)
                     /locus_tag="PLV90_01215"
     CDS             complement(14390..15274)
                     /locus_tag="PLV90_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOF95762.1"
                     /translation="MQVSNPVLIGGVPLDAFFAFILALIGFLFLGNLAYMLLRQALDG
                     RVSPGTAKWAAAILQYAVMFGGVYVSARHLLDFDPRAFAASLGILGIIVAVSSTQITQ
                     NILAGILITINRPVQLEEWIVVGELPTTGLCKVRDISFTTTILQGLDGGLILMPNSSI
                     IASKVVNYSRNRLIEIRVSLSVPVSVDIARVRDIALAVAHDDSRVIRTPGAAGRSAVH
                     DLFDLPYNRRLRSDRPDLIGLGPAVHTVSVSDGWIKMVVRIWTQKIPQKDDITSRYLR
                     EILGRLEAEDIPVKAEAA"
     gene            complement(15414..16016)
                     /locus_tag="PLV90_01220"
     CDS             complement(15414..16016)
                     /locus_tag="PLV90_01220"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOF95763.1"
                     /translation="MTLDQFRPHVQGIVQPVVDLARSIGLTPNGLTLASFFVSILAGL
                     AFYAGGIVLGVVMVALNAVFDALDGALARDMGIASPRGDFLDHVIDRYADIFIITGIF
                     AGGAAPWPIGVFALTGVLMSSYLGTQAQAVGVGRFYGGILGRADRLVLIIIAGVLTVL
                     IPGEIGGLNYLGWLLLILGVFGHYTAFQRFAHVWRQMERD"
     gene            complement(16013..16525)
                     /locus_tag="PLV90_01225"
     CDS             complement(16013..16525)
                     /locus_tag="PLV90_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase family protein"
                     /protein_id="HOF95764.1"
                     /translation="MMTGITGTPGTGKTAVAAELERRGHRIVHLADTVRPYVVEEDRA
                     RQTLVVDVDRWVEEFEPVEGIVEGHLAHLLPCDRVVVLRCRPDVLAARLAPRGYPAEK
                     IAENADAEALDVILIETLEEHPDEHILEVDTTALSVGECADRIEEFIRGELPSSYGSI
                     DWTDHLGFGT"
     gene            complement(16522..17580)
                     /gene="hisC"
                     /locus_tag="PLV90_01230"
     CDS             complement(16522..17580)
                     /gene="hisC"
                     /locus_tag="PLV90_01230"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOF95765.1"
                     /translation="MRRSVRACYAGAAGYSYAKSADDVAREHGFDRVARLASNENPRP
                     PSPAALEAAAAALREGNRYPDTRASALVEALRRYHGDYRFVTGAGMDGVIETVIRTVV
                     EPGEKVAVSTPTFSFYALAAGAHGAEVVNIPRREDFSVDPAAFAGAARGAKLAFLCSP
                     NNPTGNSVPPEDVEEILDRMDGLLFLDNAYVDFADTDYRPLMRRHDNLIIGRTMSKLF
                     ALAGLRVGYAFVPEWFEPFYHRAATPFALNSVSAAAAAGALADPGPVSEARDHVRRWR
                     ERFAGEVPFPVYPSDANFVMIDVAPLTGDEAAERLAAKGVLVRSCTSFPGLGNHYIRV
                     STGEDWENIRFIEAIRDI"
     gene            complement(17564..18688)
                     /locus_tag="PLV90_01235"
     CDS             complement(17564..18688)
                     /locus_tag="PLV90_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOF95766.1"
                     /translation="MAEDSRVLDARYYMPAFSRTMKIVRGAGSSVRDDRGREYIDCVA
                     GIAVCSTGHCHPLVVKAICDQAHELIHCSNLYYVPNQAELAEKLVEISGLSKAFFSNS
                     GAEANDGAIKLARVRTGRKKFVAFTHGFHGRTCGSLAVTHKPAIREPFEPLSVPCTFV
                     DYGDIDALAGAVDKETAGVFIEPIQGEAGVLIPPDEFLRGVRDICDDTGALMIDDEVQ
                     TGMGRTGKWFAFQHAGITPDIVTTAKGLASGFPIGALVAREGIEFKKGEHGSTFAGGP
                     LACAAALATIGVIEEVLPDVARKGERFMKGLARHHPRGRGLIIGMSVGDRCPDVQKTC
                     AENGVLVNCAADGNLRLIPPLVITDEEIDAAVGVIDAALG"
     gene            complement(18693..19610)
                     /gene="guaA"
                     /locus_tag="PLV90_01240"
     CDS             complement(18693..19610)
                     /gene="guaA"
                     /locus_tag="PLV90_01240"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOF95767.1"
                     /translation="MVDIEKFIDQAVRQIRDAAGEDKVVMALSGGVDSSVCAALAARA
                     IGDRLVPIYVDTGLMRKGETERIRSLFADTNLRTVDAADEFFAALAGITDPEEKRKAI
                     GERFIRIFEREAKRTGATLLLQGTIYPDRIESEGGIKSHHNVGGMPLNIAFKGVIEPL
                     ADLYKDDVREVAGGLSLPAEIQHRMPFPGPGLAVRVLGEVTREKIAVVREANAIVEEC
                     LVEEFHPWQCFAALLGLGTGMKGDARLHGWIVAVRAVQSRDGMTADPLPLPYEALSRM
                     VARITGEIPGVARVVYDVTSKPPATIEYE"
     gene            complement(19618..21207)
                     /gene="pyrG"
                     /locus_tag="PLV90_01245"
     CDS             complement(19618..21207)
                     /gene="pyrG"
                     /locus_tag="PLV90_01245"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HOF95768.1"
                     /translation="MKYIVVTGGVMSGLGKGITTASIGRLLKNRGYEVTAVKIDPYLN
                     IDAGTMNPAQHGEVFVLSDGGEVDLDLGNYERFLDINLKSLHNITTGKVYRNVIERER
                     HGDFLGATVQIIPHITDEIRHCIDRAAAEGINGGRVAEICLVEVGGTVGDIESMPFLE
                     AVRQMHGELAPQDMLLVHVTLVPVDTMGDHKTKPTQHSVKALRELGLQPDIIVGRSSE
                     VISPQTKKKISAFTDVPVKAVVSAKDVPDIYQIPVELEKEGLADVVCSYLALENREPD
                     PAWYRLVSQEYTNRATVAIVSKYGIEDVYISIKESLKHAGRALSTEVNIRWLDAETLD
                     LRDLADVDGILIPGGFGQRGIEGKLRAIRYARENKKPFLGLCLGFQLAVIEYARHVLG
                     IPDATSEEMGPGTHVIALLPEQEEIFNLGGTMRLGNCTVTLREDTMAARLYGKTEIVE
                     RHRHRYEVNPAFIGDLEDAGLVFSGACRDRMEVCELPDHPFYLATQFHPEFRSSPTNP
                     SPPYIGFVEACKKSRSATGIR"
     gene            complement(21344..21754)
                     /locus_tag="PLV90_01250"
     CDS             complement(21344..21754)
                     /locus_tag="PLV90_01250"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOF95769.1"
                     /translation="MGYIEELKRVGRDANPFFTLMGIELGEYGDGRASLTMEVRPDML
                     NGAGWLQGGIYVALADEAIALALCTLLADDEKIATIDEHTSFIKGVNAGTIAATGRVI
                     RKGRRVAFADGEVRDAADGALLSRTSTSFAVIGR"
     gene            21884..23116
                     /locus_tag="PLV90_01255"
     CDS             21884..23116
                     /locus_tag="PLV90_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95770.1"
                     /translation="MEDVWQPEALRNRRLEQLQATMNDYVEKNTVQIEGNLPVFFGHV
                     AATLDRTFPDLDDRSYDDFIDGITFTLLNVTREAPTVEFLEKTLRHAMGNKRRRRGRA
                     TLDVVVALKLIDTGNYVMAIDYLMRYRNYDGRVNAAIAYCHYVLSQGTAREQGLRPND
                     RELHAREEMLNLARSCAPLDRLGVFDRKDARLNRIFWFMLDLAFTWFPGEPEFYHIGI
                     VRAKHEGDIERRRHLLDLATERFSDNKYFLVEAFNTSIEQRNGSGAAAIVKQMMQQYP
                     EDHEPIYFGLKLAILGSQPGSYANFRKLAILKQFPQRLLLLLDAALEVMCNRKAEAQL
                     CFPEVKKASSGQGHYVMALEYILRDFVEGDEDRTKRARSAFFASVDQYCMLALKIGEA
                     RAAFGLQSDNPGIPSEKT"
     gene            23235..24527
                     /locus_tag="PLV90_01260"
     CDS             23235..24527
                     /locus_tag="PLV90_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF95771.1"
                     /translation="MTGNDTGSGTGRGGPDADGAREACQTLHPGESVGLSIFRELPDG
                     LVLIDGEGRCRYLNPAFTRITGYFPGDFPTLEAWFERAHPNPAYRRNVQDTMQRLLRG
                     DQSILVASVVCRSGKVRDIEFRHAPLPAGCALLVIRDVTEQVLVEEHLRQATSELTAV
                     IEAFPDLFLRLNADGTILDSRAGRLAEVPLVSRALLGRRLQELLPAEVGVILGKAMQE
                     AARTNVPAAPMEFSCAGAGKVRHYETRIMPLRELHLLAVIREITDRKEAEEELHRHRE
                     HLEDLIAERTVELERANQRLEHLLYCIEVTERRAATDWLDSSIGQGAPGTAEPDAGRI
                     TTDADGIIVIVDGTAERLTGYAGSKLAGRTVWDVLVGDAREALTVEVLGEARSVECAQ
                     GAAIVRTDGSQFPVHLLGDAIVDAGGAVIGMVCTFWKV"
     gene            complement(24538..25323)
                     /locus_tag="PLV90_01265"
     CDS             complement(24538..25323)
                     /locus_tag="PLV90_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOF95772.1"
                     /translation="MELTVLASGSKGNCAYIRGEHGALLIDAGLSARETLRRIANAGA
                     DASLIEAILVTHEHIDHVRGVDVLARKLHVPVYATGGTLEAFSEKYGPTSADVRRCRR
                     GEAFAVGDFSVEPFAASHDARDPCGFCVAEGDLRIGCCTDTGTITPAIFDRLASCDAV
                     LLESNHCSKMLEDGPYPAYLKSRIRSKRGHLSNSDAARCLQGLAGRIQMAVLAHVSEI
                     NNTPEKVLSSALEGLGFYSDQIAVTVAPQDPGPVHPETCGFRL"
     gene            25420..26811
                     /locus_tag="PLV90_01270"
     CDS             25420..26811
                     /locus_tag="PLV90_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase domain-containing protein"
                     /protein_id="HOF95773.1"
                     /translation="MSGTEGSWQGQVHRIIAGLEDGRARIDRGAVRNLVNTYAVRSLA
                     GTALRFSLPVFLPAPAGASGRGADGAAGILIEAIKAAVPVERDLGPIAGAGTEHPRHS
                     LPNVEPVTLIASGSAIGTDLWWPNAGNRIDGDGLRLVVRGALPYPGPPTPGAVKSVAE
                     CFAALLDALDRVVRRVPGRDLAAARDLSVDQKELRRALPGMGLVAFVADGTRPARRFT
                     DLRCHHRIAGPKEGVHIPFSCPRELDPVEVELAGSGRTVTGLGVRRREVFAVAGSNAE
                     GKSTFLHALIAGEDDHAAGDGREGLVSVNGIARAEAGERYLAGADVSLFFSRLPPGLS
                     GNPEAATGQGSGSLVMAHEIRTALFAAVPLLILDEDRAATNLLVPGCLQGDGVMPLAT
                     LLATRREALGETALLFAASSLDILIAQADRILVLSGHEAHAIDPREFRRRLDRHLAEM
                     REHLARREGGGPP"
     gene            complement(26861..27595)
                     /locus_tag="PLV90_01275"
     CDS             complement(26861..27595)
                     /locus_tag="PLV90_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95774.1"
                     /translation="MTKGNRYQRKDRPLKEVVSMNRSMIPLFAPVALIVACVVACGCA
                     AADDQPAAVNTTVPIEHGWNQATAAILEQYVLTRANQETYDMFNGSTVSPDPVILLDS
                     NKSPLYYRYYVQKNGKTVYAVQVSANKLLGTTVTAIGDFGYIGRDGSMTANESDSHGA
                     EAGFVRGTPVPSYTDEYTRLMLDSWEVGDTYARNVVRDAENAGIDLSTPLSDEEREVV
                     GEILREQIGQREQRIQEVEKNHRVSL"
     gene            complement(27611..>27995)
                     /locus_tag="PLV90_01280"
     CDS             complement(27611..>27995)
                     /locus_tag="PLV90_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95775.1"
                     /translation="DRVPALEAKTDREVQLTVLLPVDAAPTDYPWDHGITGYLSQMIQ
                     SAVHVGVDQGARSTLVEPALDPPFPPTGRFLDRIKNESIDLRGVGFRMRIIEPDPDQL
                     AHALQRLPELVVGHLHEVLVPPPAA"
BASE COUNT         4975 a         8992 c         9020 g         5008 t
ORIGIN      
        1 cgacggtgcc ggtgttgcgg cgagagcggt tctcaaaaaa aagtgcgggg gttcggcccg
       61 ccgcatcagt cgtagtcgcg gaagagcgcg aggttctgtc tcctgattca ccaccgccgg
      121 gtgaagtatc cggccccggc gagcacgagg acccctccgg ctatcagggc gattgtccga
      181 agcgggaagt ccggggccgc ctcgtcgacc ggcacggcca cggtctctgt tgcggtgagc
      241 tgtgcggttg tctgcgtcgc ctccgccgcc gtcggggtta ctacaggact ttcatcccgg
      301 actccgctga ttgcaaagag cgagaacccg gggcttgtcg ccccgaaggt gaccgtcgcc
      361 ccggcatcat ccacgaccca cgtcgggagg ggttcccacg ccgtgccgtt gtagcggtag
      421 aggacgatct cgtccggggc gaatccctgt tccgcgagcc acgcggtcgg gatgctgaag
      481 gtgatgtttg cgccggtgat ctcctcgaac cgggccggcg tcagttcgat gtactggtac
      541 gggcttcccg gcgccggggg gatgccgggt gcgggggagg actgtttcca cccggtaacg
      601 atcagcatgg taagccccgt gccggtcgcg gtgacggtac tcacggcgga gtctccgccg
      661 acgttatagc cgccgcccgg cccgaccgtc gagccgccgc caccgccgcc gccaccgccg
      721 gaggaggacg gtgacgtgac ggtgatggat ttacgcaggc tgtcgaatcc gaagtcgttg
      781 tatgcctgca gcgtcaccgt gtaggtgccg ggttcggtgt aggtgtgcgt ggggttctct
      841 gccgttgatc cggcgggctg gaggcgccag acgtcgttcc ggtatccgtt attgaacccg
      901 cccatgagta tgatgctccc gtccggcatc acgacgctgg cgaacatgct tcttgccggc
      961 caggcactcg cgttcacgag cgtccaggtt gcacctccgt cggtcgaccg ccagacgtcg
     1021 tcctggaatt cgccgccgtt tccgcccatg aggacggtgc ttccgtccgg catcgcgacg
     1081 gcggtgtgat accgcctccc cgtccattca gcgtgctcgg tcacctgcgt ccatgttgct
     1141 ccgccgtcgg tcgaccgcca ggtgtcgttc aggtattcgt agctctcctc accgcccgtg
     1201 agcacgatgc tcccatccgg cagcgcgaca gcggcgttgc cgtaccgtgc cgtccattcg
     1261 gcgtgctcgg tcacctgcgt ccatgttgct ccgcggtcgg ccgatcgcca gatgtcgttc
     1321 aggtatttgt cgccgttatt ccctcccatg attatgatgt tctcgtccgg cagcgcaacg
     1381 ctgctgtgga attcccttcc catccacccg gaacttgcgt tcatgagcgt ccacgatgcg
     1441 ccgtaatcgt ccgaccgcca tgtgtcgttc tggcatttgc tgccgtcggc aacccctccc
     1501 atgagcacga tgctcccatc cggcagcgcg acagcggcgt gaccgtttct taccgtccag
     1561 ttggcgtcgg gcaactgcgt ccatgttgct ccgcggtcgg tcgatcgcca ggtgtcgttc
     1621 acacgggtgg acccgctggc gatcccgccc atgattacga tgctcccgtc cggcagcgca
     1681 acgctgtcat gcaggagtct cttcggccag gcactctcgt tcacccgctc ccacggcccc
     1741 tcatacgtct cgtcgccgaa gtaccacgtc cacccgtccg ggtcgccggt cgattcatcg
     1801 gtgaacttca cggtgagcgg cgcggggccg gagagcggcg tggcgctgaa cccggcctcg
     1861 ggcggtggcg gcgtgaccgt gatataagct accttttgcg tgacgttgtg gccgtcgtcg
     1921 ttgtatgcct gcagcgtcac cgtgtaggtg ccgggttcgg tgtaggtgtg cgtggggttc
     1981 cgagtttgat ttccggcggt tttgaggcgc cagacgtcgt tcagagagac tcccatgccc
     2041 tcatcccacc cgcccatgat tatgatgctc ccgtccggca tcacgacgcc ggtgtggtag
     2101 taccgccccg tccacccggc atcaggcaac tgtgtccagg tttcgccgcc gtcggtcgac
     2161 cgccaggtgt cgttatagcc cacgccaccc ccgcccatga ttacgatgct cccgtccggc
     2221 atcacgacgc tgctgtggcc gtcccgtttc gaccattcgg catcaggcaa ctgtgtccag
     2281 ttcccaccgt aatcgtccga ttgccagacg tcgttcagga aatggtggct attgccgtcc
     2341 cagtacgtcc cgcccatgat tacgatgccc ccgtccggca tcacgacgcc ggtgtggtag
     2401 taccgccccg tccacccggc atcaggcaac tgtgtccagg tttcgccgcc gtcggtcgac
     2461 cgccaggtgt cgttatagcc cacgccaccc ccgcccatga ttacgatgct cccgtccggc
     2521 atcacgacgc tggtgtggcc ggcccgtttc gtccattcgg cactcgggtt cacgcacgtc
     2581 catgtctttc cgccgtcgtc cgaccgccag atgtccttca gggttcccgg gccgccgctc
     2641 ccgcccatga gtatgatgtt ctcgtccggc atcacgacgc tgctgtgcct gagccgttcc
     2701 atccacccgg aactttcgtt cacgcattcc catgtcgtgc cgtaatcgtc cgaccgccag
     2761 gtgtcgttat aggcatcgct atccacgtcg tacccgccca tgagcacgat gctcccgtcc
     2821 ggcatcacga cgctactgtg gtcgtaccgt ttcggccagg cactctcgtt cacccgctcc
     2881 cactccccct catacgtctc gtcgccgaag taccacgccc acccgtccgg gttgccggtc
     2941 gattcatcgg tgaacttcac ggtgagcggc gcggtgccgg tagtcacgtt gccggtgaaa
     3001 tccgccgtgg gccctgcagc gctcaccgtg ccgatagaga ttgaaaaaac cataactacc
     3061 aggcatagcc ccatcacgag ggtgcgcctg aagtgttgcc tcatcatcaa gtaatataca
     3121 atgaattcgc aacgatatgc gtttcgtttt caccccgcac agggcggcgt tttttcgaaa
     3181 aatgcgtcag tgaactaccc cgcccttcag ggcggggtag ttcactctat ggctttcggg
     3241 cctgaaacat cgtacagggc actcattggg ttcactttat tcgtgcgggc aaacggtgaa
     3301 agatgatctt gatacctacc ctctcctcct cacccgcacc gactccgcgt gagcgtggag
     3361 cccttccgca tcggcgatcg tctccaccac gtcgccgatc gcctcgagcc cctcccgcgt
     3421 gatcacctgg acggttgcgc gccggcagaa gtggttcacg tcgagaccgg agtagagccg
     3481 ggcatacccg gcggtcggga ggacgtggtt cgtccccgag gcgtaatcgc cgcaggcaac
     3541 cgccgcgtag gggccgacga agatcgaccc ggcgttcctg acccgggtga ggacggcgag
     3601 cggatcggcc acctggatcg agaggtgctc gggggcgacg gcgtcgaccg tctccaccgc
     3661 cgcatcgatg tcggcggcga cgatgtagcc cgagtggcta agtgcccgat cgatgatctc
     3721 gcgccgcacc gcgcccgccg ccatccgttt cacctcggcg ccgacacggt cggcgaggac
     3781 cgcatcggtc gtcaccagaa cgcaggcggc gttcgggtcg tgctcggcct gggcgaggat
     3841 gtcggccgcg acgaacgtcg ggtttgccgt gccgtccgcg aggatagcga tctcgcttgg
     3901 gccagccgga aaatcgatct ccgcctcgtc gcgcagaagc atcttcgccg ccgtgacgta
     3961 gacgttcccc gggccgacga tcttctcgac ccgcgggatc gtctccgtcc cgagcgccat
     4021 cgccgcgatc gcctgggcgc cgccgacccg gtagatctcg tcgaccccgg cgatatcgag
     4081 cgcgacgagc gtgatcgggt ttgtcggcgg cggggtgcag cagcagatct ccctgacccc
     4141 cgcgaccttt gccgggacgg tgcacatcag cgccgtcgag gggtatgccg cccgcccgcc
     4201 ggggacgtag gcaccgatac gggataaggg cgtcgtcctg acgccgaggg tgatcccggg
     4261 ctccatctcc tgcagccaga ggtcgcgccc gcgctgcagc tcgtggaacc ggctgatccg
     4321 ggcttccgcc tcggcgaggc tctcgatgag ttgtgcatcc acccggtcgt aggctgcctc
     4381 ccgctcctcg tcggagacgg cgatctcatc gagatcgata ccgtcaaatc tcttcgtcag
     4441 gtcgatgagg gccgcgtcgc cttccgatcg caccctcgct atgatctcag agacggggcc
     4501 cctcacccgg tcgatctccg atcgccgtcc ggcgatccag gtctcgatct ccagcgcctg
     4561 ccacatggca aaaaaggtcg gcggggcaga gcgttaaggt ttcgacaccc cggccccggg
     4621 aaggcgaaca ccgcctcacg ggcggcaggt atgtgagggc acttgacaaa tcctatttgt
     4681 taatttttat acaaaccgat aaatataatg tcggcccatc ccggcccgca tgcgatcgag
     4741 aacgtggatg cctatcctgg gcatcatggt agtgctgctt gccgcatgtg ccgtctccgt
     4801 cacggcacag caagcggcaa acccgggcgt actccggata gcgacgacga cgagcctgta
     4861 tgacaccggc ctcctcgaca accttgaaga tatgtacgag aacgtctccg gcgtggatgt
     4921 gcagatcacc gcacagggaa cgggccaggc gctcgacacc gctcggcgcg gcgacgtcga
     4981 cctcgtgctc gtccattcgc cggggcttga gcaggagttc atcgatcagg gctacggcat
     5041 caacccgcac tgctttgcct acaacaactt cctgatcgtc gggccggatt ccgatccggc
     5101 gaagatcaag aacatgacgc cggaagaggc cttcaagacg atcgccatcg ccggcatgaa
     5161 cgacacgccg ggcgtggcct tcgtctcccg cggcgacaac tcgggcacgc acaaccggga
     5221 gcagcagatc tggaaggcgg ccggttacaa ctacacgacg gacatccagg actccgggga
     5281 ctggtatgtc gagaccggga gcggcatggg ggagaccctg accatcgcga accagaagga
     5341 ggcctacacg ctctccgaca tcgggaccta ccttgccttc aagagccagc tgaacctggt
     5401 acagctcgtc accgaggggg gtgagctcat gaaccgctac agcgcgatcg cggtcaaccc
     5461 cgagaagaac ccgaatgtca acatcgacga ggcgaacagg ttcatcgact ggataacctc
     5521 caacgagacc aaggagttca tcgggaactt tggtgttgca gagttcggcc agccgctctt
     5581 cgtgccgctc tacccgcccg agtgttcgca accgccgttc aactgcacca cctgctcgtc
     5641 gggagaggtg aacgcgacag cgtgaaccgg gaacgttcgt accgtgccgc agggggaccc
     5701 gggcgcgggg ggcctctgcc gggagatcgg ggagggcggc agaaatcccc cgatctcgcg
     5761 gggacagacg gctttgaggt atgcgaaaca cctctccccg ccccgggccg ttgcggtcat
     5821 gaggatgctc cgggtgcgag gataactatt tatgatcggt ggtcgtgcta acgggctgct
     5881 atgaagatga acgttgccat tctatcggcc ggcaccatcg cgatcctctt catcctcgct
     5941 cttctggcgg gatgcacggg gacggcgccg ggcgagaacg taacaccgaa cgtaacgacg
     6001 accgcacccg ctgccggggc aaacgtgctc aagatcgcca ccacgacgag ccttgagaac
     6061 accgggctgc ttgcggagct cgagcgggtc tacgagagag agaccgggat ggacctccag
     6121 ttcatcgccc agggcacagg ccaggcgctc gacaccgctc ggcgcggcga cgtcgacctc
     6181 gtgctcgtcc acgccccggc gcttgagaag gagttcgagg acgaaggcta cggcatcgac
     6241 ccgcggtgca tcgcctacaa ctacttcatc atcgtcgggc cggaggacga cccggcgaag
     6301 atcaggaaca tgagcgcggt ggaagccttc aggacgatcg ccatcgccgg catgaacgac
     6361 acgccgggcg tggccttcgt ctcccgcggc gacaactcgg gcacccacgc ccaggagaag
     6421 gcgctctgga aggaggcggg ctacaactac acgacggaga tccttgactc cggagcctgg
     6481 tacgtcgatg ccggcaaggg tatgggcgac accctgatcc tcgcggacga gaagaaagct
     6541 tacacgctct ccgatgaggg aacctatctc tcgtttgcaa accgccttgc cctggtgccg
     6601 atcatcagcg agagcaccga gctcatgaac cgctacagcg caatcgcggt caaccccgcg
     6661 aagtacccga acacgaacgc gagaggtgcc gcggacttca tcaactggct catatccgac
     6721 gaagggaagc agctgatcgg tgacttcggg aaggtcgagt ttggaaaacc gctcttcgtg
     6781 ccgctctacg cacccgagtg cacgggaccg ccgttcaact gcacctgtgc ggaaccggtg
     6841 cccacgcccg cgtagaagcc agcagaccaa gggtagccag ggagatgtgg ggagagggga
     6901 gtgaggagac gtggtgaacg ggaacccgat cgtcgaggga ttcatcgagg ccatccggct
     6961 catcgtctcc ctcgacccgc aggtggtcga gatcaccatc cggtcgctct acgtctccct
     7021 caccgccacg ttctttgccg cgctcatcgc gcttcccctc ggggcgctga tctacttcta
     7081 cgagttccgg gggaagcatg cggtcgtctc gacgctgcag acgctgtacg cgctcccgac
     7141 cgtcatcgtg ggcctggtca tgttcctcct cctctcaaac atcggccctt tcgggttcct
     7201 ccgcctcctc tacacgcccg gcggcatgat cgtcacccag acggtcctca tcataccgct
     7261 cctcatgggg ctgacggtct cggccctctc cggtatcgac cgcgacaaac gctacacgat
     7321 caccgcgctc ggcgcaagca ggctccagac gatcgtgacc atcatcgtgg aatcccggtt
     7381 cgcggtcatg tccagcgtcc ttctcggatt cgggcgggcg atatccgagg tcggtacggt
     7441 gatgatcatc ggcggcaaca tccggggcgc cacccgcgtc ctcaccaccg cgatcgcgct
     7501 caacacatcg atggcaaact actcggtatc gatcgcgctc gggatcatcc ttctcgcggt
     7561 ggctctcggg gtgaacatcg ctctctccct ggtgcagcgg cggtgatggc atggtgatca
     7621 ttgaggcaag ggacatcagg aaagtatacg gcaacctgga ggtcctgcac gacgtcgacc
     7681 tctcggtcga ggaaggagag attctcgggc tcatcggccc gagcggatcg gggaagagca
     7741 cgcttctgcg gatactcgac ctgatcgagc cgccgagcgg cggagagctc tcggtcttcg
     7801 gcaccgatac ggtcgcggag cgcagtcgct ggctcgacct ccgccgccgc atggggatgc
     7861 tcttccagag gccgatcgtc ttcaacgcgt cggtctacga caacgtcgct atggggctcc
     7921 ggtaccgggg ggtctccggc agcgatatcg accggaaggt gaaggaagcc ctcgatgccg
     7981 tcgggctctc ccgctacatc aagagccggg cgaccgatct ctcgggcggc gagcagcagc
     8041 gggtggcgct ctcacgggtg ctcgtgacgg atcccgagat cctcttcctc gacgaaccca
     8101 cggcgaacct ggatccgggt tcgaccgcca ccatcgagcg gatcgtggcc gacctgaacc
     8161 gcgaggccgg gatgaccgtc ctgatcagca cccacgatct ggcgcagggg cagcggctcg
     8221 cccaccgcat tgcggtgatg atcgagggga cggtcgccca ggcgggctca ccccatgccg
     8281 tcttccacga gcccgggagc agaaagatcg ccgggttcgt gggcgtccag aacatcatcc
     8341 ccggccggat cgtctcccgg caagggggcc tcaccacggt ggcggcaaaa gatcggcgga
     8401 tcatctcggc gacaccgtcg cccgccgacg aggtcttcgt ggtcatccgg ggcgaggata
     8461 tatccctgca ccggcgagaa ccggggtacg aggaggcgga gaacctcttc ccggcaaggg
     8521 tcacggctat cgagtccatg gcgccgttcg tgaacgtgac cgtggattgc gggtgcgatc
     8581 tcgtcgtact gatgccggcg cggagggcgg aagccctcga gatccacgtc gggatggagg
     8641 tctgggtctc tctcccggcg aaggtggtcc acctggtccc gcaaaatggg ccgtaaactc
     8701 atatcgggac actatttgcc gaccgccgtt ctatccggac ctcctggatt aatctaaacc
     8761 gattaactct tgccttaaag gataatttta actcagttgt cccaaaaaag attatttcat
     8821 gaaatcgatt cgtatggttg cctttatcgc cctcatcgtc gccgccgcgg tcctgatctg
     8881 cggctgcacg gggacgacaa acaccgactc gacgcccgga acgggcgccc agaccacgac
     8941 ccccgcatcg acggcagcac ccacggaaaa catccaggtc aagatcttcc acgccgggag
     9001 cctgaccggg ccgtttgaga aggtgaaagc agagttcgag gccaagtacc ccggcacaac
     9061 ggtgctcctc gagcctgccg gcagcatcga ttgcgtcaag aagatcaccg agaacggcaa
     9121 acccgccgat gtcctcgcct ccgccgatta ctacctcata ccggagatga tgatacccga
     9181 tcacgccgac tggtatctca cgttcgcaaa gaacctgatg gtgctcacct atacgaacga
     9241 gagcaagtac gcaagcgaga tcaccgccga taactggtac gagatcctcg gccgtgacgg
     9301 cgtccggtgg ggcttctccg acccgaactc cgatccctgc ggttaccgca ccccgatggt
     9361 gatccagctt gccgagagcc actacaagaa cgaccagatc tttgagaacc ttgtcagcaa
     9421 gcacagcaac atcaccgtca ccgaggagaa cggcatctcc accatccacg ccggcgaccc
     9481 gaaacccgac ggcgccgtcc tgacgatccg gccgaagagc gtcgaactcg tccagatggt
     9541 ccaggccggc ggacttgact acgcctggga gtacaggagc gttgccgtcc agaacgacct
     9601 ccgctacatc gaactccccg aagcgatcga cctctcgtcg atagacttcg aggataccta
     9661 cgcgaccgtc cagattgaaa cgaagaaggg cgacagcgtc gccatcttca ccgggtcccc
     9721 catcatcttc ggcataaccg tcccgaagat cgcggagcac cccgacctcg gcgtcgcatt
     9781 catcgagatg ctgatcgaca ccaccggcca ggagatcctc atcgccgacg gccagccccc
     9841 gatcgttccc gcggacggct acggcaatgt ccctgacgtc ctcaagtcac tcgttgcgat
     9901 gagatcttga actcccctct tcttttttcc cctgcaggca ttcagtgagg cgcgcacagg
     9961 agatatcagc gattccatgg atatgaagac ccagacacgc tcaaccgacc cgcttccggc
    10021 agacggagaa ccggtcgccg ccacccggtg gaagagagag cgcgagcggt atacggactg
    10081 gtgcactctc gggttctgca tcctcggcgg gttgctcgtc ggcatcacgg tgctggcgct
    10141 cgcgaacatc acgatggcgg aactcgccga cccggcccac ctcatcaaga tcgcaacgtc
    10201 gtctacggtg atcgagtcca tcctcctcac gttcggcgcg ggggcgagcg ccgtcctcct
    10261 cctcatcgta ttcgggacgc cgcttgccta catcctcgcc cggtcccgcc cctcccgctt
    10321 caagggcgtc gtcgagagca tcatcgatat cccgctcatc ctgccgcaca ccgtggcggg
    10381 cctgctcgtc tacctcctct tcatgcgccg gggatggctc ggcgcgccgc tctcggatat
    10441 cgggcttgcg tttgaggatg cgctccccgg gacgatcgtc gctatgctct tcgtcgcctc
    10501 gccgttctac atcaacagca tgcgggaggg gtttgagaag gtgccggtcc acctggagaa
    10561 cgtcgcccgc acgctcggcg ccggcacctt caccatgttc cggacggtct cgctcccctt
    10621 gagcctccgg cacatgtaca gcggggccat actcgcctgg gggagggcta tcggggagtt
    10681 tgccggcgtc atcatgatcg cctactatcc gttcatcata tcgacgctga tctactactc
    10741 cttcacgacg gacggcatcc gtgcgagcag gagcatcgcg ttcacggtca tcctggtcag
    10801 tttcggggta ttctaccttc tccggcggat gacccggcac ctggggaggt acgatgatag
    10861 agtttgatcg ggtctcgctc gcactcggct cgttccgctt aaaggagatc agcctgacga
    10921 tcggccgggg cgattactac ttcatcgtcg ggccgtcggg agcgggaaag acggtgctcc
    10981 tcgagacgat agccggcctc caccggccgg agagcggccg ggtgctcctc cgggacgaag
    11041 agatcaccac cctccccccg gagaaacgaa gggtcgccct cgtctaccag gactactcgc
    11101 tcttcccgaa catgcgggtc atcgacaacg tctcctacgg cctccgggtg caggggatgc
    11161 gaaagaaaga ggcacgggat gaggttgttc ccctcctcga gcggttcggt atcgctcacc
    11221 tcgccgaccg ctacccggga accctctcgg gcggcgagca gcagcgggtg tcgcttgccc
    11281 gggccatcgc ggtgaaaccc gacatcctcc tcctcgacga accgctctcg gccctcgacc
    11341 ccatcacgca ggagaggttc atcggcgatc tccgccggct ccaccgggag gacgggctca
    11401 cgatcgtcca ggtgagccac tcccgccggg aggcgcacct cctcgcgacc cggatggcgg
    11461 tgatcatcga cggatacctc gtggacgaag ggaaggccgg cgtcgtcctg aacacaccca
    11521 ggagccgtga tgtcgcaacg tttgtcggta tcgagaacat cctcgatggg accgtgacgg
    11581 caaacgagga tgggcatgca acggtggatg ccggagggag gacgtttgag gcgatgaccg
    11641 acgccgctcc gggcgaggag gtcagcctct gcatccgggc cgacgatatc atccttgccg
    11701 tcgacggcga ccggcattcc agcgcccgga acacgatgac cgggacggtc gtctcggtcg
    11761 tggagaacgg ccccgtcgcc gaggtgaagg tggacgccgg cgtcgtactg accgcggtcc
    11821 tgacccggcg gtcggtacgc gatctcttgc tcgtcgcggg gaccgccgtc accctctcta
    11881 tcaaggcgac ggcgatccac gtcatccgtg cttcgtcttg atcgagagga tcaggccgac
    11941 gaagaccgcg gtgacggcgt tcgccgcgac gatggcagga tcccctctca ccacgccgta
    12001 ggcgagccag agcaggaggc cggcaaggaa gacgatgagc gtcgcaaggg agaggtcggc
    12061 ggtcgaccgc gtccgccagg cccggacgac ctgcggcgca aacgagagcg tcgtgagcga
    12121 accggcgatg aggccgagtg cggtgatggc atccatgggg ggctccatca ctatcgtgtg
    12181 ccggacctca tgaacccttg gcccggtgta tcttcaggcc ccgggctttt gcccggcacc
    12241 ctgctccttt ttccaccact ccagcgcata ggcaagggcc atgccgacga tgaacgcgat
    12301 ggtgaggccg agcgcgaggg cgaggacggc gatgactccc gtgaccaggt aggactcggt
    12361 ctttatgccg tgctttccaa gctcgatcgc gacaaagacc aggagcgcgc caaagacacc
    12421 gaacggtatg agggtcagca cctccggcgg ggcgaagacg accgcgaata cgaggatgaa
    12481 gaggccggcg acgatgttcg ccccgccggt ccttgcgccg aaccggtaca tggcggcaag
    12541 ccccccggcg ccgtggcaca tggggaaccc tccaagcggc gtcgatatga ggttcatcgc
    12601 gcctatggtc cgcgagagcc ggtcggggtc gacgcctttc ttcgggaaga ggtcgtaggt
    12661 gaggagcgat gtcgcgagga tggcgtttgc gatcgtgagc ggtatctgcg ggagtgcaag
    12721 atcccatatg ccggcgacga agtccgggag cgagggaacg atgagcggcg ggaggggtat
    12781 gagccggaac ggcggcattc cctgggtggc gatgccgacg gcaagcccga tgaggaggac
    12841 cagcagcgcg gatatatccg gtatccgggt gcgcgccgat gcgatgaaga agacgacgat
    12901 gatcgcgata gagacgatcg caaagagaac gtcgtcgacg atgtagccga gcgacgtccg
    12961 gaggagaatg agcgcaagcc ctgcctggac gccgcggatg acgcttttcg gtatccggtc
    13021 tccaagccag gtcataccgc cgacgaggcc gagcaggaga aagagcgcgc cgacgatgag
    13081 gcctgaggcc acgatctcgc cgctggagag cccgccggcg atgacgacgg cgccgatcgc
    13141 tttcatcggt tcgatcggga tgggaagccg gtagtagagc ccggcgatga tgaaccaggc
    13201 ggcgaggaag aagaagaagt ggctgacgtt cacgtccggg ctgacgatgg cgacgccgag
    13261 caggatgggg aagatggtcc cgaagtcgcc gacggctccg gcgatctctt cgagggtgaa
    13321 ccggatgccg ttccctgtac tccgggtctt ctccatcata gtcatctcct ctatctttcc
    13381 ccctttttgg cggtttcgga tggggcaccc cggcccgtca cgcgggctct gcggtcccgg
    13441 ggaaccccgg tgcagggtca tcctccggtt tcttcgcctc aaggagaccc tctgccgaga
    13501 ggtgcccgat gacggcatcg aggtactccg agacgatccg gtccttcccg gggatctggc
    13561 ggatccagat ccgcaccgtc agcctgaccc actcctcctt gatcgacgcg acctgcacct
    13621 tcggctcgaa gagccggagg tcggggcggg ggaggtcgga tgagaggcgc ccgatgtagg
    13681 ggagatcgaa gaggttctgg acggcggacc gttcggccgg ctcgacgtgg gggaggatga
    13741 ggggatggtc gcgcgcggcc gcgacggcga tctcctgcac ccgtgcgatc tcggtggtga
    13801 ccgggacggc aagcgggacc gagacctcaa gcagaccggc acgggagtag ttgatcacct
    13861 ttgaggtgat gatgcttgag ttcggcgtga ggacgaggcc gccgtcgagg gcctggagga
    13921 tcgtcgtcgt gaaggagatg tcctgcaccc tgcagaggcc ggtctccggc ctgccgctga
    13981 cgaccaccca ctcctcgagc tggatagggc ggttgatcgt gatcaggacc cccgcaacgg
    14041 cgttctgtat gatctggcgc gacgagaacg cgacggcgat accgagtata ccgagcgatg
    14101 cgaagaaggc cgtcagttcg aagtcgagga agtaccgggc gcttgcatac accccgccga
    14161 cggtgatgcc gtactggaga atggccgccg cccatttgac ctttccgcgg gaggcgcgac
    14221 cgtcgagcag ccgccggagg agcatgtagg cgaggttgcc aaggaagagg gatacgatga
    14281 aggcgaagat gaacgcgagg aacgcatcga ggggtacgcc gccgatgaga accgggacac
    14341 tctcctgcat gtgtagaaaa gtgagcgata agaggattaa tctcttccgt caggccgcct
    14401 cggccttgac cgggatatcc tcggcctcaa ggcgcccgag gatctcgcga aggtagcggg
    14461 aggtgatatc atccttctgc ggtatcttct gggtccatat tcgcaccacc atcttgatcc
    14521 agccgtcgct gaccgatacg gtatggacgg cgggtccaag gccgatgaga tcggggcgat
    14581 cggaacggag ccgccggttg taggggaggt cgaagaggtc gtgtacggcc gaccgtccgg
    14641 cggcgccggg agtccggatg acccgggagt cgtcatgcgc caccgcaagc gcgatatcgc
    14701 gcacccgtgc gatatcgacg gagaccggga cggagagcga gacccggatc tcgatcagcc
    14761 tgttccggga gtagttgacg accttggatg cgatgatgct tgagttcggc atgaggatga
    14821 ggccgccgtc gagcccctgg aggatcgtcg tcgtgaagga gatgtcgcgc accttgcaca
    14881 gccccgtcgt cggtagctcc ccgacgacga tccactcctc gagctggacc gggcggttga
    14941 tcgtgatcag gatgccggca aggatgttct gggtgatctg ggtcgacgat acggcgacga
    15001 tgataccgag tataccgagc gatgcggcaa aggccctcgg gtcgaagtcg aggaggtggc
    15061 gggcgctcac atagaccccg ccgaacatga cggcgtactg gaggatggcc gccgcccatt
    15121 tggccgtccc cggggagacc cggccgtcga gggcctgccg gaggagcatg taagcaaggt
    15181 tgccgaggaa gaggaacccg atgagggcga ggatgaacgc gaagaacgca tcgaggggta
    15241 ccccgccgat gagaaccgga ttgctcacct gcatgtggga agaggtgggc cggcaggaga
    15301 gttaacttct cctctctcgg gcggctcaag gatatcgaga ggtcctccgt tccgggccgg
    15361 tttgcgcggt gttgtgccgc tctcgcgcga gggaaccggc agcaccgtcc gcgtcagtct
    15421 ctctccatct gccgccagac gtgggcgaac cgctggaatg cagtgtagtg tccgaagacg
    15481 ccgaggatga gaaggagcca cccgaggtag ttcaaccccc cgatctcccc ggggatcagg
    15541 accgtgagca cgccggcgat gatgatcagc acaaggcggt ccgcgcggcc gaggatgccg
    15601 ccgtagaacc tgccgacccc gaccgcctgg gcctgggtgc cgaggtagga ggacatcagg
    15661 acaccggtca gggcaaagac cccgatcggc cagggtgcag cgccgcccgc aaatatgccg
    15721 gtgatgatga atatgtcggc gtagcggtcg atgacgtggt ccaggaaatc cccccgggga
    15781 ctcgcgatcc ccatatcccg ggcaagcgcc ccgtcaagcg catcaaagac ggcgttcagg
    15841 gcaaccatga cgacgccgag cacgatcccc ccggcataga acgcgaggcc ggcaagtatc
    15901 gatacgaaga acgacgcaag ggtgagcccg ttcggggtga gcccgatact ccgggcgagg
    15961 tctaccacgg gctgaacgat gccctgcacg tgcggccgga actggtcaag tgtcatgtgc
    16021 cgaaacccag atgatcggtc cagtcgatgg acccgtagga ggatggcagt tctccccgga
    16081 taaactcctc gatccggtcg gcgcactctc cgacggatag ggcggtcgta tccacctcga
    16141 ggatatgctc atcggggtgc tcctccagcg tctcgatcag tatgacgtcg agcgcctccg
    16201 cgtccgcgtt ctccgctatc ttctccgccg ggtaacccct cggggcgagg cgcgcggcga
    16261 ggacgtccgg gcggcaccgg agcaccacca cccggtcgca ggggagaagg tgcgcgaggt
    16321 ggccctcgac gatcccctcg accggctcga actcctccac ccaccggtcg acgtccacca
    16381 cgagcgtctg ccgggcgcgg tcctcctcga cgacgtaggg ccgcaccgta tcggccaggt
    16441 ggacgatccg gtggccccgc cgctcgagtt cggccgcaac ggccgtcttc ccggtccccg
    16501 gcgtcccggt gatgcccgtc atcatatgtc gcggatcgcc tcgatgaacc ggatgttctc
    16561 ccagtcttcg cctgtgctga ccctgatgta atggttcccg agcccgggga aactggtgca
    16621 cgaccgcacg aggacgccct tcgccgcgag gcgctccgcc gcctcgtcgc ctgttaaggg
    16681 ggcgacgtcg atcatgacga agtttgcatc cgaaggatag accggaaacg gcacctctcc
    16741 ggcaaaccgt tcccgccacc gccggacgtg gtcgcgggcc tcgctgaccg gtcccggatc
    16801 ggcaagcgcc ccggcggcgg ccgccgccga gaccgagttc agggcaaacg gtgttgccgc
    16861 ccggtggtag aacggctcga accactccgg cacgaaggcg tagccgaccc ggaggccggc
    16921 aagggcgaat agcttcgaca tcgtccgccc gatgatcagg ttatcgtggc gccgcatgag
    16981 ggggcggtag tcggtatccg caaaatcgac gtaggcgttg tcgaggaaga gcagcccgtc
    17041 catgcgatcg aggatctcct cgacatcctc cggcgggacc gagtttccgg tcgggttgtt
    17101 cggggagcag aggaaggcaa gtttcgcccc acgcgctgcc ccggcaaaag ccgccggatc
    17161 gaccgagaag tcttcccggc gggggatatt cacgacctct gcgccgtgtg ccccggccgc
    17221 gagggcgtag aacgagaagg tgggggtcga gacggccacc ttctcgccgg gctcgacgac
    17281 cgtccggatc accgtctcga tgacgccgtc catcccggcg ccggtgacga accggtaatc
    17341 gccgtggtag cggcggagcg cctcgacgag ggccgatgcc cgcgtgtccg ggtagcggtt
    17401 cccctccctg agggcggccg ctgccgcctc gagcgcggca ggcgagggcg ggcgggggtt
    17461 ctcgttgctt gcgaggcggg ccacccggtc aaaaccgtgc tcccgcgcga cgtcgtcggc
    17521 cgacttcgcg taggagtagc ccgccgcgcc tgcatagcac gccctaaccg agcgccgcat
    17581 cgatgacacc gacagccgcg tcgatctcct catccgtgat aacgagcggc gggataagcc
    17641 ggaggttccc gtcggcggcg cagttgacga gcacgccgtt ctcggcgcag gtcttctgca
    17701 catccgggca ccggtcgccg accgacatgc cgatgatcag gccgcggccg cggggatggt
    17761 gacgggcaag ccccttcatg aaccgctcgc ccttccgggc cacgtcgggg agaacctcct
    17821 cgatgacgcc gatcgtcgcg agcgccgcag cgcaggcgag cgggcctccg gcaaacgtgc
    17881 tcccgtgctc gcccttcttg aactcgatcc cctcgcgggc gacgagcgcg cctatgggaa
    17941 acccgctcgc gagtcccttt gcagtcgtga cgatgtcggg ggtgatgccg gcatgctgga
    18001 aggcgaacca tttgcccgtc cggcccatac cggtctgcac ctcgtcgtcg atcatcagcg
    18061 cgccggtatc gtcgcatata tcccggacgc cccggaggaa ctcgtcgggc ggtatcagga
    18121 cgcctgcttc gccctggata ggctcgatga agaccccggc ggtctcctta tcgaccgcgc
    18181 ccgcgagggc gtcgatgtcg ccgtagtcga cgaaggtgca ggggaccgag agaggctcga
    18241 acggctcccg tatcgcgggc ttgtgggtga ccgcaagcga cccgcatgtc cggccgtgga
    18301 acccgtgggt gaacgcgacg aacttcttcc tccccgtccg gacgcgggcg agtttgatcg
    18361 cgccgtcgtt tgcctccgcg ccggagttcg agaagaacgc cttcgaaagg cctgatatct
    18421 cgacgagttt ctccgcgagt tccgcctggt tcgggacgta gtagaggttt gagcagtgga
    18481 tcagctcatg ggcctggtcg cagatcgctt ttaccaccaa cgggtggcag tggccggtgc
    18541 tgcagaccgc aatgcctgcc acgcagtcga tatactcgcg cccccggtcg tcccggacgc
    18601 tcgaccccgc gccgcggacg atcttcatcg tccgggagaa cgcgggcatg tagtagcgtg
    18661 catcaagcac gcgtgaatct tctgccatag tatcactcat actcgatcgt tgcggggggt
    18721 tttgaggtga cgtcgtagac cacccgggcg acgccgggga tctcgccggt gatccgggcc
    18781 accatccttg ataacgcctc gtaggggaga gggagcggat cggcggtcat gccgtcgcgg
    18841 gactggactg cccggacggc gacgatccag ccgtggagcc gggcatcgcc cttcatgccg
    18901 gtcccaagcc cgagaagcgc ggcaaaacac tgccaggggt ggaactcctc caccaggcac
    18961 tcctcgacga tggcgtttgc ctcccggacg accgcaatct tctcccgcgt cacctcgccg
    19021 agcacccgga cggcaagccc cgggcccggg aagggcatcc ggtgctggat ctccgccggg
    19081 agcgatagac cgccggcgac ctcgcggaca tcgtccttgt agaggtccgc aagcggctcg
    19141 atgacgcctt tgaatgctat gttgaggggc atcccgccga cgttgtggtg gctcttgatg
    19201 cccccctcgc tctcgatgcg gtcggggtag atcgtcccct ggaggaggag cgttgctccg
    19261 gtccgctttg cctcccgctc gaatatccgg atgaaccgct cgccgatggc cttgcgcttc
    19321 tcttcggggt cggtgatgcc tgcgagcgcc gcgaagaact cgtccgcggc atccacggtg
    19381 cggaggtttg tatcggcaaa gagggagcgg atccgctccg tctcaccttt gcgcatgagg
    19441 ccggtatcga cgtatatcgg gacgagccga tcgcctatcg cgcgggcggc gagcgccgca
    19501 cagaccgacg agtccacgcc gccggagagc gccatcacga ccttatcctc gccggcagca
    19561 tcacgtatct gccggactgc ctgatcgata aacttctcga tgtctaccat actgttttca
    19621 cctgatcccg gtagcggacc tgctcttctt acatgcctcc acaaacccga tgtagggcgg
    19681 ggaggggttt gtcgggctcg acctgaactc cgggtggaac tgggtcgcaa ggtagaaggg
    19741 atggtccggg agttcgcaga cctccatccg gtccctgcac gcgcccgaga agacgagccc
    19801 ggcatcttcg aggtcgccga tgaacgccgg gttcacctcg taacggtggc ggtgccgctc
    19861 cacgatctct gtcttcccgt agaggcgggc cgccatcgtg tcctccctga gggtgacggt
    19921 gcagttccca aggcgcatcg tgccgccgag gttaaagatc tcttcctgct cggggaggag
    19981 agcgatgacg tgcgttcccg gccccatctc ctcgctcgtg gcgtcgggga tgccgaggac
    20041 gtggcgcgcg tactcgatca ccgcgagctg gaacccgagg cagaggccga ggaagggttt
    20101 cttgttctcg cgggcatacc ggatcgcccg tagcttcccc tcgatcccgc gctgcccgaa
    20161 cccgcccggt atcaggatgc cgtcgacgtc ggcgagatcg cgaagatcga gcgtctccgc
    20221 atccagccag cgtatgttca cctcggtgga gagcgctctt cccgcgtgct tgagcgactc
    20281 cttgatggat atgtagacat cctcgatacc gtacttgctc acgatggcga ccgtcgcccg
    20341 gttcgtgtac tcctgcgaga ccagacggta ccacgcgggg tccggttcgc ggttctcaag
    20401 ggcgaggtaa ctgcagacga catccgcaag cccctccttc tcgagctcca ccggtatctg
    20461 gtagatgtcg gggacatcct ttgcggatac gacggccttg accgggacgt cggtgaaggc
    20521 ggatatcttc ttcttcgtct gcgggctgat cacctcgctg ctcctcccta cgatgatgtc
    20581 gggctgcaac ccgagttcgc ggagcgcctt gaccgagtgc tgcgtgggtt tggtcttgtg
    20641 gtcgcccatg gtatccaccg gaaccagggt gacgtggacg aggagcatat cctgcggcgc
    20701 aagttccccg tgcatctggc ggacggcctc aagaaacggc atgctctcga tatccccgac
    20761 cgtgccgccg acctccacga ggcagatctc cgcaaccctg ccgccgttga tcccctccgc
    20821 cgccgctcga tcgatgcagt gccggatctc gtcggtgatg tggggtatga tctggacggt
    20881 cgcgccgagg aagtcgccgt gccgttctct ctcgatgacg tttcggtata ccttcccggt
    20941 cgtgatgtta tggagcgatt ttaagttgat atccaggaat cgctcgtagt tgccgagatc
    21001 gagatcgacc tctccgccgt cggagaggac gaagacctcc ccgtgctggg cggggttcat
    21061 ggtgccggca tcgatgttga ggtacgggtc gatcttgacc gccgtcacct catagccgcg
    21121 attttttaag aggcggccga tggatgcggt ggtgatgcct ttcccgagtc cgctcatgac
    21181 gccgccggtt acaacgatat acttcaaact ctcgttcccc tccggttctt ccagggtctc
    21241 tcagtcatgt atgggatctc tggcattgag aatgtattgt tccggcactc ccccgggaga
    21301 atccctgccg gtgcgacggc cgatctctct gcacaccgcg gcgctacctc ccgatgaccg
    21361 caaacgatgt cgacgtccgg gagaggagcg cgccgtccgc cgcatccctg acctcgccgt
    21421 cggcaaacgc aacccggcgg cctttgcgga tgactctccc cgtggcggcg atcgtccccg
    21481 cgttgacgcc ttttatgaaa ctggtgtgct cgtcgatcgt ggcgatcttc tcgtcatcgg
    21541 caagaagcgt gcagagcgca agtgcaatcg cctcgtctgc aagcgccacg tagatcccgc
    21601 cctggagcca cccggcgccg ttcagcatat ccggccggac ctccatcgtg agactcgcgc
    21661 gcccgtcgcc gtattcgccc aattctatcc ccatcagcgt aaagaatgga tttgcatccc
    21721 ttcccacccg cttcagttct tctatgtagc ccacgcgacc accatcctcg cggaaaggtt
    21781 cgaggccggc cgggataagg cttgccggca attcgcccgg aggccctgga gcgccagaaa
    21841 aaatatagga acaaatatct tcccggatct ccacctgtag ggtatggaag acgtgtggca
    21901 gcccgaggcg ctccggaaca ggagacttga gcagctccag gctaccatga acgactacgt
    21961 cgagaagaac accgtccaga tagaaggaaa cctgccggtc ttcttcgggc acgttgccgc
    22021 caccctcgac cggacgtttc ccgatctgga tgaccggtcg tatgacgact tcatcgacgg
    22081 gatcaccttc acgctcctca atgtgacgcg cgaggccccg acggtggagt tcctcgagaa
    22141 gaccctgcgg cacgctatgg ggaacaagcg gcggaggaga gggcgggcaa ccctcgacgt
    22201 ggtggtggcg ctcaaactga tcgataccgg caattacgtc atggcgatcg attacctgat
    22261 gcgttatcgt aactacgacg gccgggtcaa cgccgcgatc gcctactgtc actacgtcct
    22321 ctcgcagggg accgccagag aacagggcct tcgccccaac gaccgggaac tccacgcccg
    22381 cgaggagatg ctgaaccttg cgcggtcgtg tgcgccgctc gaccggctcg gggtcttcga
    22441 ccgcaaagac gcccgcctga accggatctt ctggttcatg ctggacctgg cgttcacctg
    22501 gttccctggc gaacccgagt tctaccacat cgggatcgtg cgggcaaagc acgaggggga
    22561 tatcgagcgt cgccggcatc tcctcgatct cgcaacggaa cggttctcgg acaacaagta
    22621 tttcctggtc gaggcgttca acaccagcat cgagcagcgc aacgggagcg gtgcggccgc
    22681 catcgtcaag cagatgatgc agcagtaccc ggaagaccat gaaccgatct acttcgggct
    22741 gaaactcgcg atactcgggt cgcagccggg gtcctacgcc aacttccgga agctcgcgat
    22801 cctgaagcag tttccccagc gcctgctcct gctgctcgat gccgccctcg aggtgatgtg
    22861 caaccgaaaa gccgaggcgc aactctgctt tccggaggtg aagaaggcct cttctgggca
    22921 ggggcactac gttatggcgc tcgagtacat cctccgtgat ttcgtcgaag gcgacgaaga
    22981 tcggacgaag cgggcacgat ccgctttctt tgcaagcgtc gaccagtact gcatgctcgc
    23041 cctgaagatc ggggaggccc gagccgcctt tgggctgcag agcgataatc ccgggatacc
    23101 ctcagaaaaa acctgatcgc aacgataaaa gggaaaggta caacgatgaa tttatcaaga
    23161 gtaacaggca aatccctctg cagggagcgt ttgccggccc ggatatacgg atgtgccggg
    23221 gagggttggt taccatgacg ggaaacgata cgggttcggg caccggccgg ggaggaccgg
    23281 atgccgacgg cgcacgggag gcctgccaga cgttgcaccc gggagagagc gtcggcctct
    23341 cgatcttccg cgaactgccg gatggtcttg ttctgataga tggtgaaggg agatgccggt
    23401 acctgaaccc ggcgtttacc cggatcaccg ggtacttccc gggggatttc ccgaccctgg
    23461 aggcctggtt cgagcgtgcc caccccaatc ccgcataccg ccggaacgtg caggatacga
    23521 tgcagaggct tctccggggc gaccagagca tccttgtcgc gagcgtggtc tgccggagcg
    23581 gaaaggtgcg ggatatcgag ttccggcatg cgccgctccc tgccgggtgc gccctgctcg
    23641 tcatccgcga cgtcaccgag caggtcctgg tcgaggaaca cctccggcag gccacatccg
    23701 aactgacggc ggtgatcgag gcgttccccg acctcttcct ccgcctgaac gccgacggca
    23761 cgatcctgga ctcacgggcc gggaggcttg cggaggtgcc tctcgtctcc cgggcgctgc
    23821 tcggccggcg tctgcaggag ctgctccccg ccgaggtcgg ggtgatcctc gggaaggcga
    23881 tgcaggaggc cgcccggaca aacgtgccgg cagcgcccat ggagttctcc tgtgcgggag
    23941 ccggaaaagt ccggcactac gagacccgga tcatgccgct gcgcgagctg catctccttg
    24001 ccgtcatcag ggagatcacg gaccggaaag aggcggaaga ggaactgcac cgccaccgcg
    24061 agcacctgga agacctgata gcggagcgga ccgtggagct cgaacgcgca aaccagcggc
    24121 ttgagcacct cctctactgc atcgaggtga cggaacgccg ggccgcaacc gactggctgg
    24181 actcatcgat cgggcagggc gccccgggaa cggccgaacc cgacgcgggg aggatcacga
    24241 cggatgcgga cggtataatc gtcatcgtcg atgggacggc cgaacgcctg accggctacg
    24301 ccggaagcaa gctggccggc cgcacggtat gggatgttct tgtcggcgat gcccgggaag
    24361 ccctcaccgt ggaggtgctc ggcgaggcga gatccgtgga gtgcgcgcag ggtgcggcga
    24421 tcgtgaggac cgacgggtca cagttcccgg tccacctcct gggcgatgcg atcgtcgacg
    24481 ccgggggcgc cgtgatcggc atggtctgca cgttctggaa ggtgtgaggg cgcgcggtca
    24541 gagccggaaa ccgcaggtct cgggatgcac cgggccggga tcctggggcg ccacggtgac
    24601 ggcaatctgg tcggagtaga acccgagccc ctcgagtgcg gaggagagca ccttctcggg
    24661 cgtgttgttg atctcgctga cgtgggcgag caccgccatc tggatccggc cggcaagccc
    24721 ctggaggcag cgggcggcgt cggagttcga gaggtgcccg cgtttcgacc gtatacgact
    24781 cttgaggtac gcggggtagg ggccgtcctc gagcatcttc gagcagtggt tgctctcgag
    24841 gaggacggcg tcgcaggagg cgaggcggtc gaatatggcg ggggtgatgg tgccggtgtc
    24901 ggtgcagcac ccgatgcgga ggtcgccctc ggcgacacag aacccgcagg ggtcgcgggc
    24961 atcgtgggaa gcggcgaagg gttcgaccga gaagtcgccg acggcgaacg cctcgccgcg
    25021 ccggcatcgc cggacatctg ccgacgtcgg cccgtatttc tccgagaacg cctcaagcgt
    25081 gccgccggtc gcgtaaaccg gaacgtgaag cttccgcgcg aggacgtcga cccccctgac
    25141 gtgatcgatg tgttcgtggg tgacgaggat cgcctcgatg agcgatgcgt cggcgccggc
    25201 gtttgcgatg cggcggaggg tctcgcgtgc ggagagcccc gcgtcgatga gcagcgcacc
    25261 atgctccccc cggatatagg cgcagtttcc cttgcttccg ctcgcaagga cggtcaattc
    25321 cattatacaa ttggtggtct gcgcggctat ttaagcacgc ggcggggagg gtgtacaaag
    25381 agccttgtgg ttcgggatac tatctcctga cgagaggtaa tgagcgggac ggagggctcc
    25441 tggcaggggc aggttcaccg gatcatcgcg gggcttgagg acggtcgggc acgcatcgac
    25501 cggggagcgg tgcggaatct cgtcaacacc tacgccgtcc ggtcgctcgc tgggacggcg
    25561 ctccggttca gcctcccggt cttcctcccg gcgccggccg gggcatccgg ccggggtgcc
    25621 gacggggctg ccgggatcct catcgaggcg atcaaggcgg cggttcccgt cgagcgggac
    25681 ctcggcccga tcgccggcgc cgggacggag cacccccgcc actctctccc gaacgttgaa
    25741 ccggtcacgc tgatcgcgtc agggagcgcc atcgggaccg acctctggtg gccgaatgcc
    25801 gggaaccgta tcgatggcga cggcctccgc ctggtcgtca ggggcgctct cccttacccc
    25861 ggcccgccga caccgggcgc ggttaagagt gtcgcagaat gctttgcagc gctcctcgat
    25921 gctctcgacc gcgtcgtgcg ccgggtgccg ggccgtgacc ttgccgcggc gcgggatctc
    25981 tccgtcgacc agaaagaact ccggcgcgct ctcccgggga tggggctcgt cgcgttcgtc
    26041 gcagacggca cgcgcccggc ccgcaggttc accgacctcc ggtgccacca ccggatagcg
    26101 gggccgaagg agggcgtgca catccccttt tcgtgcccgc gggaactcga cccggtcgag
    26161 gtggaacttg ccgggagcgg ccggaccgtg acggggcttg gggtccggcg gcgggaggtc
    26221 ttcgcggtcg caggctcgaa cgccgagggg aagagcacct tcctccacgc gctcatcgcg
    26281 ggagaggacg accacgccgc cggggacggg cgggagggat tggtcagtgt gaacgggatc
    26341 gcccgggcgg aggcgggcga gcgctacctt gcgggggccg acgtcagcct cttcttctcc
    26401 cgcctcccgc ccgggctttc gggcaatccg gaggcggcaa ccggccaggg gagcgggtcg
    26461 ctcgtcatgg cgcacgagat ccggaccgcg ctctttgccg ccgtgccgct cctcatcctc
    26521 gacgaggacc gcgccgcgac gaacctgctg gttccgggct gcctccaggg cgacggggtg
    26581 atgccgcttg cgaccctgct tgcgacccgg cgcgaggcgc tcggcgagac ggcgctcctc
    26641 tttgcggcct cgtcgctcga tatcctgatc gcacaggccg accggatcct ggtgttgtcg
    26701 ggccatgaag cgcacgcaat cgatccccgt gagttccggc ggcgtctgga ccggcacctc
    26761 gcggagatgc gggagcacct ggcgaggagg gaggggggtg gtccgccgtg aactcgcgca
    26821 gagaacgcga agattgggag atgggtgtgc gacccggctg ttagaggctc acccgatggt
    26881 tcttttcgac ctcttgtatt ctctgttctc gttgcccgat ctgttctcga aggatctctc
    26941 cgacgacctc ccgttcctca tcggagagcg gcgttgagag atcgattccg gcattttctg
    27001 cgtctcgcac aacattccgg gcataggtgt ccccgacttc ccaggaatcg agcatcaatc
    27061 gagtatactc atcggtatac gaggggaccg gagtaccgcg gacaaacccg gcttctgcgc
    27121 catgcgaatc gctttcgttc gcggtcatcg atccgtctcg ccctatgtaa ccgaaatcgc
    27181 cgatcgcagt caccgttgtg ccgagcagtt tgttcgcgct cacctggacc gcgtaaacgg
    27241 tcttgccgtt cttctgaacg taataccggt aatacagggg gcttttgttg gaatcgagca
    27301 ggattacagg atccggagat accgtcgaac cgttgaacat atcgtatgtt tcctggttgg
    27361 ccctggtaag cacgtattgt tcaagaattg ccgctgtcgc ctggttccat ccgtgttcta
    27421 tagggacggt tgtattgacg gctgcaggct gatcatcagc tgctgcacag ccgcatgcaa
    27481 cgacacaggc cacgatgagt gctaccggtg caaacagggg gatcatgctg cggttcatgg
    27541 atacgacctc tttgaggggt ctatctttgc gctgataacg gttgcccttc gtcatggcgt
    27601 tctcccgcgg tcaggcggcc ggcgggggaa ccagaacttc gtgcaggtgc cctaccacaa
    27661 gttccggcag acgttgaaga gcatgtgcga gttgatcggg atccggttcg atgattctca
    27721 ttctgaagcc tacacctcgc aggtcgatgc tctcgttctt gatccgatca agaaaccgcc
    27781 ctgtaggcgg aaacggcggg tcaagcgcgg gctctaccag agtgctccgg gcaccctgat
    27841 caacgccgac gtgaacggcg ctctgaatca tctgagaaag atagccggtg attccgtgat
    27901 cccacggata atcggtaggg gccgcgtcaa ccggtagtag gaccgtcaac tgcacctctc
    27961 gatcggtctt tgcctccagt gccggcaccc gatcg
//
LOCUS       DAOSII010000008        27767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_1920_length_27767_cov_51.762486, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000008 DAOSII010000000
VERSION     DAOSII010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 27767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27767
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1920_length_27767_cov_51.762486"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..443)
                     /locus_tag="PLV90_01285"
     CDS             complement(51..443)
                     /locus_tag="PLV90_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95776.1"
                     /translation="MLERAGYIQRGRCAAVDGEGRSSRIVEAVLNGRQFGVRVWELRQ
                     ALDGRRPARVEVLRRDWGPVLGGTVGRAEVLGAKTAITLTLVTGERYTVPIVALRALL
                     ARAAAFAPVAAVRSRGDSSTMGQALTPG"
     gene            complement(516..1913)
                     /locus_tag="PLV90_01290"
     CDS             complement(516..1913)
                     /locus_tag="PLV90_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95777.1"
                     /translation="MPVPDLFSAMMKEEWRIHATIFGSAGFAFFPVVVAIFAFAGSLA
                     LPLFSDTLPSTVMAMLALAFFLLAGVMVGSFGTMGREFMNRRFGQASLVAYASRSLPL
                     SERRIFATFIVKDTVYYILLYVLPAAAGFTAAAPFIALNPIWGLRAFVSLALAFLIGL
                     SAVCLLSTLYARSIGLLAGTVVVLAGAGLAALGMNAVFPPYAFLIDPAPGSLALSLIL
                     ILVPAAASVLLVTVDYPDRTKRFTNRFEPLAERLCPLGSAHFIAKDALDLSRSEGGAG
                     KVIFSFILPLGLVWVCLRILIRFIPGIDPLVVFAILLGVISATIYNWLTEFDSFTSYT
                     FLPVEVSAVIDGKLKSYAIANLLPVAVLVLAAASAGGAGSLLPALAAFLSVSVYTLAV
                     TVYLCGLSPNIMLYHAWVFLRYVLMISPALLLLIFASLLDPAYAFGSLFLVPAAGLLI
                     ARARSKWQAWDMPGY"
     gene            complement(1900..2616)
                     /locus_tag="PLV90_01295"
     CDS             complement(1900..2616)
                     /locus_tag="PLV90_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF95778.1"
                     /translation="MIEATELTKNYGNVAAVNGISFSVMEGELFGLLGPNGSGKTTMI
                     RMLTGQVRPTAGSARVMGLDPAEDPIGVRQLIGIVPEQETPPSFLTAEEYLHFVARIR
                     NLDRIDERCDRWFDLLGFQEKRDVLCKDLSRGTRQKLMVAQAFLHEPRLVLIDEPLIN
                     LDPIVQRTVKDFLQEYVRDGGTVLISTHILEIAEEICDRVGVLHDGKLLYCGQTEGLA
                     GPGRSLERSFLDLVRGDAGA"
     gene            3199..5406
                     /gene="glgP"
                     /locus_tag="PLV90_01300"
     CDS             3199..5406
                     /gene="glgP"
                     /locus_tag="PLV90_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOF95779.1"
                     /translation="MKTEIPYDRFAHVPERIFGLVDLAYNLWWSWHSSASILFKLLNR
                     AEWKMSRHNPVRMLLETPPRFFERAATNPEYLRRYDIIMFRFREYMEPRTSWFAEHYP
                     GRVPLTIAYLSSEFGLHHSLPFYAGGLGILAGDHIKASSDLGVPMVAVGFMYAEGYLH
                     QHMEADGWQKNVAEILDRDAAPVLRVLDDNGRHLVVRVPLIDPPIYVAVWKVQVGRVP
                     LYLLDTHIDENLPENQSISHRLYLKELECRLRQEIVLGIGGRKVLHALGVDYSAMHLN
                     EGHSAFAILERLRERLVAGMPPDEALEKVRGTSIFTTHTPVPAAHDIFPEDLMARYFR
                     SYCPSLGMTWDEFMALGKDPHNPGARFNMTAFALRMSLFHNGVSRRHGEVAREMWQPL
                     WPDLPLEEVPIDAITNGVHLPSWLNHRIEALFDRYMDVIYPNWREDYDNPIIWEVVDE
                     IPDNELWGEHQWLKMKLFARLRERKRLKWAGHREEPANLAAEGLMLDTSALTIGFARR
                     FAEYKRADLIFEDLDRLDSIVNNRWRPVQFIFSGKAHPADEKGKRILQRIYQYSQDPR
                     FGGRISFIEDYGEQLARYMVQGVDVWMNTPVPLMEASGTSGMKAGMNGVLNLSVLDGW
                     WVEGYNGRNGWGFEGHTPYAGNNRADADTIYDLIENEVAPLYYSRTMDNVPHKWVQMM
                     KESIKSIAPEYCSFRMLKDYIRSYYPSVCMYAAEPERQLAGIEGVCLPTNPFR"
     gene            5547..6416
                     /locus_tag="PLV90_01305"
     CDS             5547..6416
                     /locus_tag="PLV90_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOF95780.1"
                     /translation="MTGSRSARKVLIAVAVAAMLGAALIVAPPATPATVLLLFTIGIV
                     AGIFGGLIGVGGSTIMLPIMYFYLGFPEPIAVGTGLFVVIFTSLSGAAGHLVRGNLDR
                     RVAGWIAAGGLVGVIFGSWLFSFLVGYTRLLSLVLGLVFLVPSLGMIRDGLRPATRPE
                     PGSIGGTPPGHLLFGTGVGVLTGITGLGGGYALVPGLLYIFGAPVAVTMGTSLASMIP
                     MAILGGGIKLVEGYVAVGAGLTLAAGSIAGAQAGAATIHRFRPETLKLIFGFYFLYAA
                     IRFITGFFGVGVP"
     gene            6479..7438
                     /locus_tag="PLV90_01310"
     CDS             6479..7438
                     /locus_tag="PLV90_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95781.1"
                     /translation="MEWKDLLPVLAGGLVILAVALVVKPALVGGPATPPGSLPPAYVP
                     PAPLPAATPYPEDSAVYTRDFRWTAIDGGVLTTEVRVPEALFRDHRETPRIEDRLAYG
                     RYVLADADRPILEDLVRQIAPPVASPDAEYYRLMNVVFFVQQIPYEYDNSTNSYIEGV
                     LPRHALHIGDGIEYQKYPAEMLVDGKGDCEDAAILMAGLIDALGYDAVLLRYRGDTKL
                     GIAGHMALGIQMNEFNPYYAKYTPRYFEHGGKHYYYIEGTDYLKFSAGNVTSWGKPFP
                     IGDVDGTGIEAVRSRIPEIIPLRYTPAPAEYRIRSVGLPAGGG"
     gene            7444..8040
                     /locus_tag="PLV90_01315"
     CDS             7444..8040
                     /locus_tag="PLV90_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95782.1"
                     /translation="MDITVPKLPTLPKLPAIATNRRQALIAVGAVIAVVAAAFIGWTL
                     FTDWTESRQVAVFGSHVAASEADLARVSAKISSHMSFRPEAPSIAESDVVMREFAAIA
                     TYGQAVTTYHRRIIGADEVPEAYIGAQSAYIRALDCLNRAFSLWASAAAAYDAGAYTS
                     AKDALVKADQAWKEYVAATGDYNRELRRAEEGEEAPPV"
     gene            complement(8037..8690)
                     /locus_tag="PLV90_01320"
     CDS             complement(8037..8690)
                     /locus_tag="PLV90_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HOF95783.1"
                     /translation="MQGRHILLLILLTATILACGCTADGERSETQPASLTPAPSSAQP
                     QMSGDGMSAPTPAAPVRGNATQEVRKVEFVDPAVYRIPTPTPTIAIGKQPDDIKVSEK
                     MVDYARATINYPPGVLATETYHIPFPYWAFNVSITPMGEHPWLTIDVHEKDDPNRAVI
                     ETIRYSRNSFPYEGGMPREKKERFMILEGFRDYYFIIRSESVKSFELTILIPEKYLV"
     gene            complement(8824..9324)
                     /locus_tag="PLV90_01325"
     CDS             complement(8824..9324)
                     /locus_tag="PLV90_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOF95784.1"
                     /translation="MQKTIERREVQEETTAEVRELVHVMLNADTYTSLQAISTLGAIG
                     APAVGPLVETLLAAGSDARWTVAMALARTGEPAVEPLIGVVLVADDELKNPAIWALAE
                     IGDPRAVNPLVGTLKSGQSECCRALTAAALLKLGDPAGIAEVWKVFDREGEPFRGLAM
                     EALEGT"
     gene            complement(9453..11369)
                     /locus_tag="PLV90_01330"
     CDS             complement(9453..11369)
                     /locus_tag="PLV90_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF95785.1"
                     /translation="MQSEQDIPGRILRSLKFRPKGMTITEIAKTLKSNRNSVSKHLEV
                     MQAAGQVEARLVGNAKVYSVAQRVPLSAFLCFTKNLILVLDADLTIVQANDQCLRRFG
                     RTKEELVGRNLREAALPVVSSLKSLALVENLEREQVIADIPHRRNDGSESFYQMQAIP
                     TTFESGERGCTLILEDITERKRYIRNTEFLARTAIDLVDLQPVEDIYQYIADRLAELV
                     PGAYAYILSYSEAEHQFAIRATAGEGFREGLTELLGRNPVGQILPVTIVFEAPYHQTP
                     LSMRGLQEFVLRPEPSAWPFYDICFRAIPEEICEAILERFNIVKICLTGLVWRDRLYG
                     ITGIFLPPGREVENKEIIESFIRQVSIAIARRETEERLRLSGVRFRSMLDASPFGIAL
                     IDHERRFCYCNRRFSEIFGYETGRAVTATEWVRQIFADDSDWQEAVAAWRSDLERSAP
                     GQIRPRTFVVRSRDGAKKTILSRAVTLPDGTEYVTCEDITEEARAYRLLVADIADLRR
                     QEQELLIKDRAIASTRRAVALLAPDGVVTYANAAYLALWGYPAAVEVQGSPFSGFWEH
                     PDKIRMIIRAVIGGGAWHGELVGLRKDGTRFTVDLLGTPIVAESGRVIGMMAAATRLP
                     KEQGKPEKTRNAPT"
     gene            11578..12315
                     /locus_tag="PLV90_01335"
     CDS             11578..12315
                     /locus_tag="PLV90_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOF95786.1"
                     /translation="MEEHELVSITQGGFAIMNPTTPEKVLAAGRAAGLRKGSRVVEIG
                     CGNGTILALWGREYGVSGLGIESREDACRRAEETFRAAGPGGRLRVRCMDAREYVSDE
                     PFDCAASIGASHIHGGFAATLDALLDLVHDEGSVVIGDRFWRSDRIPPEFARAWPDVP
                     TGYEVLGTAREAGLDLAAVIRADGADWDRYESGIWQSVLSWLGAHPDHPDRDSMIDYL
                     HRLQDEYFGYGREYMDWALFVMVPGFW"
     gene            complement(12341..13996)
                     /locus_tag="PLV90_01340"
     CDS             complement(12341..13996)
                     /locus_tag="PLV90_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOF95787.1"
                     /translation="MRTPACIAILLVAVVVLGAGCVGESQPPPEEAEKSVLLEAVARI
                     NGELESINASIGESARVLGETGLRSAAGKEAVQRAMLNHPYTESTLVATKEGIVTMAV
                     PESYAGTVGSDISSHAETERSVQEQVPLVSEVFPLAEGYAGVAQSYPIFGAGGEYIGF
                     VSVAYRSDALIGRAIVPLINGTPYDIWVTQTDGLVVYDTTPEEIGRNLFADPVYQAPG
                     LQEAFSRIVAEPSGSLEYSFWERNWERNVTKKAIWDTAGISGTEWRVVATRSADAGEV
                     PATTGERPPVEDTAGDMKAFVGEAAAYAREHGRTDALAAFNNPAGEFVRDELYIFAYD
                     MNGTVLALPFQQALIGADRSAVHDVSGVAYIAGMKRLAAEGGGSINYIYPNPARGYAE
                     DLKLGYVLPVDETWFVGSGVYIPGVAATFNTSVRETLVQRVKAARDYAQKQGRAAACA
                     AFNDVSGDFADGEAYIFAYTMNGTTLALPYQPEYIGENRFDLEDRYGVRIILLESAAA
                     ESGGGFVYITYYNPDTGVDGLKLCYVAPVDGEWFVGSGVYAGA"
     gene            complement(14403..15656)
                     /locus_tag="PLV90_01345"
     CDS             complement(14403..15656)
                     /locus_tag="PLV90_01345"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HOF95788.1"
                     /translation="MKILIVDPGCSGVDGSILLASLIDLTGEPRALDPLAGAIRDLGI
                     CREFRYEAERVGAGEEVAATLLSITAREEGAMAGDDFQEACMAVADDVGLSPGARDRV
                     SSVLGDLTAVSFRPAETIFSVVGTMFLLDRAEFFEGTIVAMPPALGATITRTDCGDIP
                     GPAPTTLAVLTRHRFPFASVPAEADLTTPVGAALLAAITERSISLYPAMTPARVGYGI
                     RPSGEPTGLLRVMEGESLPPGEDRVVMLETNLDDTSGEVIGYTIERLFAEGAADVFVT
                     PALGKKNRPVSVVSVMAAAADENRLIRILMEETGTLGVRVREFRKVVADRRKETVPVS
                     VGGREYPIRVKTASANGRPVAEKPEYDDLAAIARNRGIPLRLVGDEVRLCLSERRRER
                     TAREGGAGGGCPPDLPSAANTARDG"
     gene            complement(15669..17510)
                     /locus_tag="PLV90_01350"
     CDS             complement(15669..17510)
                     /locus_tag="PLV90_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95789.1"
                     /translation="MEIERERVLLAAAHAVTYEPDYMTVDKLEAATRGHGALVIPAFA
                     AANSLAGDIFCSIGAPEMRLMDMAVVDASAPSLPLDSVIAKAVGEAKRGGAEPANAAL
                     IVATLAYFSGSCARAGVPLGNRKLGAIARMHAGGDRTSAIALTTNKFTHRVPAFPAYL
                     AIYEALMGKRLTRLDGAVLPPFVAGGGVMGHAALGEDYNIPELARNAAMVGTRALMRS
                     MEGAGTTPYPLWPALIAAAVTMELVHPDALLGEEFGKFGEVDSSYLAGKGAVEAAGLP
                     KKIHMRGTGEEYETARLVGDFGLILKDVGGPSVIGSMVTNELFAGFQESAAIGAGFSG
                     GPVNVPLGHLAGDILPALRLLIGNGGDIERTAAAIRDYKMQSFIDPETALCATNTITR
                     KAEVINRGPVTDACIRASEPTRNRAIYRRAVKVHEMLKEGRTLEEAAGALDEEKKALI
                     ERRGSAILSGFAGKEITFRFTEIAPHARRTDPLTARFWGFDAKVSYDVRIDGESYHIE
                     NLTARVIPEYALEGKGRDDPGMGTAIFAGAVLTQELQYGGNTILNITVPAAVAAALGT
                     SPAEAATGAAKGARLTNAIPGGRAVAERVATIAQDIVANLGKPDEGA"
     gene            complement(17598..18089)
                     /locus_tag="PLV90_01355"
     CDS             complement(17598..18089)
                     /locus_tag="PLV90_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF367 family protein"
                     /protein_id="HOF95790.1"
                     /translation="MIPLYAYRDNTCDPRKCTVKKLARRGLATIVTNIAKIPRSTLLL
                     DPTAERAVSPADRHLPSITALDCSWEVLDTGAVASWRNRRALPFLVAANPVNFGRPFR
                     LTSVEAMAATLSILGEEEQARTILAPFGWGLRFLEVNAEPLEDYAQAKDSAEVVAIQA
                     LYM"
     gene            18125..18964
                     /locus_tag="PLV90_01360"
     CDS             18125..18964
                     /locus_tag="PLV90_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HOF95791.1"
                     /translation="MYVLIAPCIENPACRARGITTDEDLRHFIRAVERCRHFSIDMVP
                     LPCPETIYIGADRPPGSYLDRLATPAFAALLDRLEAEVRAVIRERGEAPLALVGVDSS
                     PACGVTATYYSSVKQPGRGAFLARFPDIPAIDVKEFARYRIYLAGPLFSEAERAYNLI
                     LHDLLAVHLFDVYLPQEAGDTDPARCGEEHGAIFAQNLLALRDADVVVAVIDGADADS
                     GTSWEMGYAYALKKKIVALRTDFRVVGHHERVNLMLEESAAVVTKKEDLPRALGSLLP
                     ASP"
     gene            complement(18956..19366)
                     /locus_tag="PLV90_01365"
     CDS             complement(18956..19366)
                     /locus_tag="PLV90_01365"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF95792.1"
                     /translation="MAELTVRAELGALTAIADFLAGTLAGSADDMVFSVQLAVDEACS
                     NTILYGYPNREAGAITIACTVDDDVIRVTITDDGVAFDPLTAPPPLLDIPAEERPIGG
                     LGIHFIRTVMDSVAYARRDDRNVLSMEKRRPASG"
     gene            complement(19366..20733)
                     /locus_tag="PLV90_01370"
     CDS             complement(19366..20733)
                     /locus_tag="PLV90_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOF95793.1"
                     /translation="MAFSEFGDLFFVLFQMICVILVAAYLITRTASFTRVLDGLVTWK
                     SQAILILLFGALSIYGTESGITILGATANVRDLGPMVGGLACGPVVGLGAGLIGAAYR
                     FSLAGFTAVPCSAATILAGLFGGLIFLLSGRKFVGIHGAVLFAIGMEAFHMGITLLLC
                     RPFDQALTVVREVALPMIIANAIGVFIFALIIGNLITERQTKAERDAYLSELERKKAE
                     LRIAHDIQMSFLPERLPDVPGLDIAAFSLPAKEVGGDFYDAIPLSGQRIAFVIADVAG
                     KGVPAALFMALSRTVLRANALIPRSARDAVREANALIAADAKSGMFVTLFYAVVDPVK
                     KTLTYVNAGHNPPLLFRSGSERVLELKGTGIILGVMPDADYGEETLALEKNDLVLLYT
                     DGVTEAIDSHEKQFGEQRLIETVTGCHDLPSREIVDRIRRAVADFSGDEPQFDDQTLM
                     VLRVV"
     gene            complement(20739..21095)
                     /locus_tag="PLV90_01375"
     CDS             complement(20739..21095)
                     /locus_tag="PLV90_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOF95794.1"
                     /translation="MSERVDILERRAGAVDIIAVKGRLDAGSSEKAEERINRVLDAGG
                     RYLLINLCDLDYISSSGLRVLLAALKRLKKDGGALRIACAQPQILEVFTMAGFHRIFS
                     LSPDEATALAGFPAGP"
     gene            21600..23276
                     /locus_tag="PLV90_01380"
     CDS             21600..23276
                     /locus_tag="PLV90_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF95795.1"
                     /translation="MDPTTGALSLLLSNLKEHPRGMSVSDLAAAVGINRNTVSRYLDV
                     LLVSGQVEMETYGKAKVFYLSQRVPIAAMLNFSSDLVLSLDRELRIVQANDAVCAFAG
                     RERDDIIGNGIEASPLAAFDHPLIRGRITDALNGREVTEELRFLRHDRELFFRAKFLP
                     TVFNDGAPGVTIILEDITEERRAEEALRESELLFRSLVENISDLILNVDETFRFTYVS
                     PKSREILGYAPEEMLHKTPCDFMAPENAERARAQFSALLADPEPRILFEWTMLHRDES
                     DVILDVSGTPVYDVIGDFTGYRVVCRDVTERARAAKRVAQWKSFLYSVVNNVPGMVFV
                     REVEGNTFVFANRAAEIFLGMTQEEMAGRQAADIFPPEMAVFFADGDRELVERAAALE
                     KEIRISGGRTLSMKKIPIFNSRGVLRYMLGIAEDITDRSAAKDLLVAERDRAQGYLDA
                     AGVMIAVIGADGTIDLINRRGSELLGYAGDDLIGKNWFATVVPERLRDPLARNFARLV
                     AGRIEPPAFEESPVVTRDGQERLIRWHNALLRDADGKVVAMVSSGEEPAG"
     gene            23376..23954
                     /locus_tag="PLV90_01385"
     CDS             23376..23954
                     /locus_tag="PLV90_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOF95796.1"
                     /translation="MVTTLVDIISLVLIAVVVLIVIGIVAVMIGIYNRFFSLKNSADA
                     TVGQVKVAMKKRLDMIEQLLGAVKSYAAFEKDTLTGVTAMRARIGSAGPGDLNELERE
                     SRSVLGRLIAVAENYPNLKTSQTVQDLMGAVKGIEDEIARHRYTYNNIAQQYNTMTDT
                     IPSNLVASVMGFKKLEYLEFGEDIERVPTIAF"
     gene            23961..25787
                     /locus_tag="PLV90_01390"
     CDS             23961..25787
                     /locus_tag="PLV90_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOF95797.1"
                     /translation="MRVTEQQQILILVAITLLIGVLAVAGAVALPGLLQGDLDVGHYD
                     AVFYENGTLIERYTYDVRAAGEYRMLFRYWDAPLTFAAIDRPHVEFLGMTAPPGVIGY
                     ARDSGGEVRTAAGSATPSDLATIRNLALDSEVGIFNPGYFAPGTYTVEYRYLVRPPVE
                     YDSQWAHLNLKLVDEHVPFRNLRITLPFAGDIEEVYTHPPTLDIERTADAVVITGSAS
                     QNDALNVELILVPAFTEKVGGFGEFVPDVRQKAADANRWPPLLYGAANILYGLSILLV
                     LATPFILLGVYLRYGREKPFTVPEYLSFTPNTALKPWQVNLLFKGDALEFDDNGFYAT
                     VLDLHRQKKIVVTEKSGGNGVTVRIVSGESSDPYEQRVLNFLANIADDHVVDTADLET
                     FAETARRSPGYQHRILQYQQSLAALTRNVDTTLARQYVSDGRGLILPLIFLGAIPCGL
                     AILTIIFAPGAAYLLIPAGILSFIVAVQVGIAALFPSTLFGVWKGDRYREKLEWDAFA
                     HFLSDLALIRQYSPADLSMWGEWLVYGTALGLGDKVEQAMKNLNISLPDVGMPLYSNM
                     PVIFAPIVFYSPPSSGGGHGGFGGGGSFGGGGGFGGGGVGGR"
     gene            25888..27066
                     /locus_tag="PLV90_01395"
     CDS             25888..27066
                     /locus_tag="PLV90_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HOF95798.1"
                     /translation="MRGSYLNYSAVVLALLSVAVACGCMAGGEKDDARDRISGNGTIT
                     YLDLEGGFYGIVADDGSRYLPADLPADFRQDGLRVAFVVDRAEETATIQQWGTPVDIV
                     SMEKGDARRTVTANGTVTYIDLEGGFYGIIAEDGMNCLPANLPEEYRIDGLSVRFSAD
                     LEEDVVGFPMWGIPVNIRTIEPSGGVRLVAGNGTITYVDLEGGFYGIVADDGEQYFPL
                     GLSEAWLVDGMDVAFVARAREDVAAIGQWGAPVDVIAIDKAGSATFVAENGTVTYIDL
                     EGGFYGIIADGGRHYLPLGLEERYRVDGMRVAFAGKIARDIVTIQQWGTPVEILAVPW
                     ACSSCGGSAGIANPAAAWCLAQGHAYEIRKNPDGSEYGVCIFANGTVIDEWDYYRQSH
                     "
     gene            27269..27625
                     /locus_tag="PLV90_01400"
     CDS             27269..27625
                     /locus_tag="PLV90_01400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOF95799.1"
                     /translation="MDVTVSERDDTAIVSVDGRIDAESAENLDTAISGVLARGGRRVL
                     VNLEKAAYMSSSGLRVLLGKLRALKGVDGEMALCSLQPSVYQVFLLAGVHEVFPIYVG
                     LDEILVGTEDEEKESP"
BASE COUNT         4894 a         8795 c         8875 g         5203 t
ORIGIN      
        1 cggcgcactg ggacgagtac gcaaagaggc gggtgacggc atgatcgtgg ctacccgggt
       61 gtcagggcct gccccatggt gctcgaatcg ccccgggagc gaaccgccgc gacgggggca
      121 aacgcggcgg cacgtgcaag aagtgcccgg agcgccacta tcggcaccgt gtagcgctcc
      181 ccggtgacga gcgtgagcgt gatcgccgtt ttggccccca agacctctgc gcggccgacc
      241 gtgccgccga ggaccggccc ccagtcccgg cgaagcacct ccacccgggc agggcgccgg
      301 ccatcgagcg cctgccggag ttcccatacc cggaccccga attgccgacc gttgagaacg
      361 gcctcgacga tccgggagga ccgcccctcc ccgtcgaccg cggcgcacct ccccctctgg
      421 atgtaacctg ctctctccag catatgctat ctgtctccct cacgactcta aaagccgcgc
      481 cgtgcggtgt gaaagtgtaa ggtggggatg gggtctcaat accccggcat atcccacgcc
      541 tgccacttcg accgggcgcg ggcgatgagg agccctgccg ccggaacgag gaagaggctc
      601 ccgaaagcgt acgcgggatc gaggagcgag gcaaatatca ggaggaggag ggccgggctt
      661 atcatgagga cgtagcggag gaagacccag gcgtgataga gcatgatgtt cggggagagc
      721 ccgcagaggt agaccgtcac cgcgagggtg tagacggaga ccgagaggaa ggcggcgaga
      781 gccgggagga gagaccccgc accgccggca ctcgccgccg cgagcacgag gacagcgacc
      841 ggaaggaggt ttgcgatggc gtagctcttc agttttccat cgatcaccgc ggatacctcc
      901 accggcagga aggtgtagga ggtgaaggag tcgaactccg tcaaccagtt atagatggtc
      961 gccgatatca cgccgagcag gatcgcaaag acgacgagcg ggtcgatccc cggtatgaac
     1021 ctgatcagga tccggaggca gacccagacg aggccgagag ggaggatgaa cgagaagatc
     1081 accttcccgg ccccgccctc gctccgggag aggtcgaggg catcttttgc gatgaagtgc
     1141 gcgctcccga gcgggcaaag cctctccgcg agaggttcaa acctgttcgt aaacctcttc
     1201 gtccggtccg ggtagtcgac ggtgacgaga aggaccgatg cggcggccgg gaccaggatg
     1261 aggatgagcg agagcgcgag cgatcctgga gccggatcga tgaggaaggc atacggggga
     1321 aagacggcgt tcattccgag agctgcaagc ccggcacccg cgaggaccac gaccgttccg
     1381 gcaaggagcc ctatggagcg ggcgtagagg gtcgagagga ggcagaccgc ggagagaccg
     1441 atgaggaagg cgagcgcaag tgagacaaac gcgcgaaggc cccagatcgg gttgagagcg
     1501 atgaacgggg ccgccgccgt gaacccggct gcagcgggga gaacgtagag caggatgtag
     1561 tagacggtat ccttgacgat gaacgtcgcg aatatccgcc gttccgagag cgggaggctc
     1621 cgggaggcgt aggcgacgag gcttgcctgc ccgaaccggc ggttcatgaa ctcccgcccc
     1681 atcgtcccga acgaacctac catcacgccc gcaagcagga agaaggcgag cgcgagcatg
     1741 gccatcacgg tagaggggag cgtatcggag aagaggggga gggcgagcga gcctgcgaag
     1801 gcaaagatgg cgacaaccac cggaaagaac gcaaagccgg cgctcccgaa gatcgtcgcg
     1861 tggatccgcc actcctcctt catcatcgca gagaagaggt caggcaccgg catcgcctcg
     1921 caccaggtcg aggaaggaac gctcaaggct gcggccgggc ccggcgagcc cctctgtttg
     1981 gccgcagtag aggagcttcc cgtcgtggag gaccccgacc cggtcgcaga tctcctcggc
     2041 gatctcgagg atatgggtgg atatgaggac ggtcccgccg tcacggacat actcctgcag
     2101 gaaatccttg accgtccgct gcacgatggg gtctaagttg atcagcggtt cgtcgatgag
     2161 gacgagtcgg ggttcgtgga ggaaggcctg cgcaaccatc agtttctgcc gggtgccccg
     2221 cgagaggtcc ttgcagagga cgtcgcgctt ctcttgaaac cccagcaggt cgaaccagcg
     2281 gtcgcaccgc tcatcgatgc ggtcgaggtt tcggatgcgg gccacgaagt ggaggtactc
     2341 ttcggcggtc aggaaactcg gcggcgtctc ctgctcggga acgataccga tgagttgccg
     2401 gaccccgatg gggtcttctg ccggatcgag ccccatcacc cgtgccgatc ctgcggtcgg
     2461 ccggacctgc ccggtcagca tccggatcat cgtcgtcttc cccgacccgt tcggcccgag
     2521 gaggccgaag agttcccctt ccataacaga gaacgatatc ccgttcacgg cagcgacgtt
     2581 gccgtagttc ttcgtgagtt ctgttgcctc gatcatctgt cgcacgtgag tcaccctcca
     2641 ccagtctcca gggaaacatc agcagcaggc ccctataata ctttttcatc cgcgtcagga
     2701 gggctgatgc aggatcccca aaaacaggcc acatatccgg ccacctccct cctctcaccc
     2761 ctgtgccggc accggcccgc gtctcccggc ggcagcctgc cctgctggag cgcacggcag
     2821 gtataagcac cctcgcagat cgctggagcc catggatgaa caggaacgat acgcccgcgc
     2881 gaggaggcgg gtcggggaga tccggggact tttacatgca tcttacagtc tatatcatct
     2941 tcaacgtctt ctccttcatc ctcagtatgg tgaccgaccc cggggtgctc tggtctttct
     3001 gggtggcgtt cttccgggga tcggccttgt actccatgcg ctcgacacgt atgccggggg
     3061 caggttctcc ggcagagagt gggaagggcg aaagatccgc gatatcgtgg agcgcgaaga
     3121 gcggcggtga cccggttccc gcccggccgg ccgggtgatg gaagcactta tcaggtgcga
     3181 caggcaatgc ccgttgcgat gaagacggag ataccctatg accggttcgc ccacgtcccg
     3241 gagcggatct tcgggctggt ggaccttgca tacaacctct ggtggagctg gcactcctcc
     3301 gcaagcatcc tcttcaaact cctcaaccgg gcggagtgga agatgagccg tcacaacccc
     3361 gtcaggatgc tgcttgagac cccgccgcgg ttcttcgagc gggcggcaac gaacccggag
     3421 tacctccgcc gctacgatat catcatgttc cggttcaggg agtacatgga gccgaggacg
     3481 agctggtttg cggagcacta tcccgggcgg gtgccgctga cgatcgctta cctctccagc
     3541 gagttcgggc tgcaccactc gctcccgttc tatgcggggg ggcttggcat cctcgcaggc
     3601 gaccacatca aggcatcgag cgacctcggt gtgccgatgg tcgccgtcgg gttcatgtac
     3661 gccgagggat acctccacca gcatatggag gcggacggct ggcagaagaa cgtcgcggag
     3721 atcctggacc gcgatgccgc gccggtcctc cgggtgctcg atgataacgg ccggcacctt
     3781 gttgtccggg ttcccctgat cgaccccccg atctacgtcg cggtctggaa ggtgcaggtg
     3841 gggcgcgtcc ctctctacct cctcgacacc cacatcgacg agaaccttcc cgagaaccag
     3901 agcatatccc atcgcctcta cctaaaagag ctcgagtgcc ggctccggca ggagatcgtg
     3961 ctcggtatcg gcgggaggaa ggtgctccac gccctcggcg tggactactc ggcgatgcac
     4021 ttgaacgagg gccactcggc gtttgccatc ctcgaacggc tccgggagcg cctcgttgcc
     4081 gggatgcccc cggatgaggc gcttgagaag gtccggggca cgtcgatctt caccacccat
     4141 acccctgtcc cggctgccca cgacatcttc cctgaagacc tgatggcgag gtacttccgc
     4201 agttactgcc cgtcgctcgg tatgacatgg gacgagttca tggcgctcgg gaaggacccg
     4261 cacaatccgg gcgccaggtt caacatgacc gcgtttgccc tccgtatgag tctctttcac
     4321 aacggcgtct cgcggaggca cggcgaggtc gcccgggaga tgtggcagcc actctggccc
     4381 gacctgcccc tggaggaggt cccgatcgac gccatcacaa acggcgtcca cctgccctcg
     4441 tggttgaacc accgtatcga ggcgctcttt gaccgttaca tggacgtgat ctacccgaac
     4501 tggcgagagg attacgataa cccgatcata tgggaggtgg tcgacgagat accggacaac
     4561 gaactctggg gcgaacacca gtggctgaag atgaagctct tcgcccggct ccgggagagg
     4621 aaacggctca aatgggcggg acatcgggaa gaacccgcga accttgccgc cgaggggctg
     4681 atgctcgaca cgtcggccct gacgatcggg tttgcccgcc ggttcgccga gtacaagcgg
     4741 gcggacctca tattcgagga cctggatcgg ctcgacagta tcgtgaacaa ccgctggagg
     4801 ccggtgcagt tcatcttctc ggggaaggct catcccgccg acgagaaggg caaacgtatc
     4861 ctgcagagga tctaccagta ctcccaggat ccccgattcg ggggccggat ctccttcatc
     4921 gaggattacg gcgagcagct ggcgcgctac atggtccagg gcgtcgatgt ctggatgaat
     4981 acacccgtcc cgctcatgga ggcaagcgga acgagcggca tgaaggcggg gatgaacggg
     5041 gtgctgaacc tctcggtcct ggatggctgg tgggtcgagg gctacaacgg caggaacggg
     5101 tgggggtttg agggtcacac cccctatgcc gggaacaacc gggcggacgc ggatacgatc
     5161 tacgacctta tcgagaacga ggtcgccccg ctctactact cgcggacgat ggacaacgtc
     5221 ccgcataaat gggtccagat gatgaaagag tcgataaaga gcatcgcccc ggagtactgc
     5281 tccttccgga tgctcaagga ttacatcagg agctactacc cctcggtctg catgtatgcg
     5341 gccgaaccgg agagacaact ggccggcatc gagggggtct gcctccccac gaaccccttt
     5401 cggtaggttc atggagaatc gggggcgtac aacagatgca tgctgccccg cccgatgatg
     5461 atccggcctg agacgagaga ggattacccg gcggtctgcg ttctggcctt gcgtgcgttc
     5521 gtgctccgtg atgagagttc tgacgcatga cggggagccg atctgcccgg aaggtgctga
     5581 ttgccgtggc agtcgcggcg atgcttggcg cagcactcat cgtcgctccg ccggcgacgc
     5641 cggcaaccgt gctcctcctc tttacgatcg gcatcgttgc cgggatcttc ggcggcctca
     5701 tcggggtcgg cggatcgacg atcatgctcc cgatcatgta cttctatctc gggtttcccg
     5761 aacccatcgc cgtcgggacc ggcctcttcg tggtcatctt cacgtcgctc tccggcgctg
     5821 cgggccacct ggtgcggggg aacctggatc gtcgtgttgc aggctggatc gccgccggcg
     5881 gcctggtggg ggtcatcttc ggttcctggc tcttctcctt cctggtcggc tacacccgcc
     5941 tcctcagcct cgtcctggga ttggtcttcc tggtcccctc cctcggcatg atccgtgacg
     6001 ggctccggcc cgccacccgc ccggagcccg ggagcatcgg gggtacaccc cccggccacc
     6061 ttctcttcgg caccggggtc ggtgtcctga ccggtataac cgggcttggc ggcggctacg
     6121 ccctcgttcc cgggctgctc tacatattcg gcgccccggt cgcggtcacg atgggaacgt
     6181 cgcttgcatc catgataccg atggcgatcc ttggcggcgg gatcaagctc gtggagggtt
     6241 acgtcgccgt cggggcggga ttgacccttg ccgccgggtc gatcgccggc gcccaggcgg
     6301 gggctgcgac gattcaccgg ttccggccgg agaccctgaa actgatcttc ggtttctact
     6361 tcctctacgc tgcaatccgg tttatcaccg ggttcttcgg cgtcggggtg ccgtgagtca
     6421 aggtttttag cgggcaaccg cccatatcct agtatcgata cgcagtggga gatcggatat
     6481 ggaatggaaa gatcttctcc ccgttcttgc cggcgggctt gttatcctgg ccgtcgccct
     6541 cgtagtgaaa cccgcgctcg tcggaggacc tgcaactccc cccggttcgc tcccgccggc
     6601 atacgtcccg cccgctcctc tgccggcggc gacgccctac cccgaagaca gtgcggtcta
     6661 cacccgggat ttccgctgga cggcgatcga cggcggcgtc ctgacgacag aggtcagggt
     6721 tccggaggcg ctctttcgcg atcaccggga gacgccgcgg atcgaagatc gtcttgccta
     6781 tggacgatat gtgcttgccg atgccgatcg gccgattctt gaagatctcg tccgccagat
     6841 cgctcccccc gttgccagtc cggatgcaga gtactatcgg ctgatgaacg ttgtcttctt
     6901 tgtccagcag atcccctacg agtatgataa cagcacgaac tcttacatcg aaggcgtcct
     6961 cccccggcac gccctgcaca tcggtgatgg tatcgagtat cagaagtatc ctgcggagat
     7021 gctcgttgac gggaaaggtg actgtgagga tgccgccatc ctgatggcgg ggctcatcga
     7081 tgccctcggc tacgatgcgg tgctgctgag atatcggggc gacacgaaac tcggtatcgc
     7141 gggccatatg gctctcggta tccagatgaa cgagttcaat ccctactacg cgaagtacac
     7201 gccgaggtac ttcgagcacg gagggaaaca ctactactac attgagggga cggactatct
     7261 caaattctcc gcggggaacg tgacgtcctg ggggaaaccg ttccccatcg gggatgtcga
     7321 cggcaccggc atcgaggccg tgaggtccag aatcccggag atcatccccc tgcgttatac
     7381 ccccgcaccg gccgaatacc gtatccgctc cgtcgggctg ccggcaggag ggggatgagg
     7441 accatggata tcactgtccc gaaacttccg actctcccga agcttcctgc catcgcgacc
     7501 aaccggcggc aggcgctcat cgccgtcggg gcggtgattg ctgtggtagc cgccgccttc
     7561 atcgggtgga cactctttac cgattggacc gaaagccggc aggttgcggt ctttggcagc
     7621 cacgtggcgg catcggaggc cgaccttgcc cgggtctccg cgaagatatc ctcccacatg
     7681 agttttcgcc cggaggcccc gtcgattgcg gagtccgatg ttgtaatgcg ggagtttgcg
     7741 gccatagcga cgtacgggca ggcggtcacg acctaccacc ggcggatcat cggggccgac
     7801 gaggtccctg aagcgtatat tggagcgcag tcggcctaca tccgggcgct cgattgcctg
     7861 aaccgcgcgt tctccctctg ggcgtcggcc gcggccgcct acgacgcagg agcctatacc
     7921 tcggcaaaag atgcccttgt aaaggcagat caggcatgga aggagtacgt cgccgcgaca
     7981 ggagactaca accgggagct ccggagagca gaagaagggg aagaggcccc tcccgtctag
     8041 acgagatact tctccgggat gagaatggtg agctcgaacg actttacgga ctcagaacgg
     8101 atgatgaagt agtaatcccg gaatccttcg aggatcatga atcgctcttt cttctctctg
     8161 ggcattccgc cctcgtacgg aaagctgttt cgggagtaac gtatggtctc gatgacggcc
     8221 cggttggggt cgtccttctc atggacatcg atcgtgagcc agggatgttc gcccatcggg
     8281 gttatgctca cgttgaaggc ccagtagggg aagggtatgt ggtaggtctc ggtggcaaga
     8341 acgccgggag ggtaattgat ggttgccctg gcgtaatcca ccatcttctc ggagaccttg
     8401 atgtcgtccg gctgctttcc tattgcgatc gtgggcgtcg gggtcggtat ccggtagacc
     8461 gccgggtcga cgaactccac cttcctcact tcctgcgttg cgtttccccg gacgggcgca
     8521 gccggcgtgg gggcgctcat cccgtcaccg gacatctgcg gctgcgctga tgacggcgcc
     8581 ggggtgaggg aggctggctg cgtctcgctc ctctcgccgt ctgccgtgca accgcaggcg
     8641 aggatggtcg ccgtaagaag gatcaggagc aggatatggc gcccttgcat gatgatctct
     8701 tgttttgcgg cgttattaga agttactctt tcggacggcc gaaaaaaccc ccgtacacgt
     8761 ttccgggggg tgtcggtgca tccggacggg attgcaaaaa gagatctgtg ccggtggggt
     8821 gcttcacgtt ccctcaagcg cctccatggc aaggcccctg aacggctcgc cctcccggtc
     8881 gaacaccttc cacacctcgg cgatgcccgc cgggtcgccc agtttcagca gggctgctgc
     8941 ggtcagggcc cggcagcact ccgactggcc gcttttgagg gtgccgacca gtggattgac
     9001 cgcccggggg tcgccgatct cggcaagcgc ccagattgcc gggttcttga gttcgtcatc
     9061 cgcgacgagc acgaccccga tgagcggctc caccgccggc tccccggttc gggcgagcgc
     9121 catcgccacg gtccagcgtg catcgctgcc ggcggcaagc agggtctcca ccagcggccc
     9181 gaccgccggc gccccgatgg ccccgagggt gctgattgcc tgcaggctgg tgtaggtgtc
     9241 ggcgttcagc atgacgtgta cgagctcccg gacctctgca gtggtctcct cctgtacctc
     9301 tctcctctcg atggtttttt gcatctcttc aacctcctgg tatggacgca ggatgttctg
     9361 gatgtaaaat aagcgtttac gaaccagaat cgctgtgcgg tactgcagac cggcactgcc
     9421 gggcatagat ttttatatcc ccggagctac ggctatgtcg gagcgtttcg tgtcttctcc
     9481 ggtttcccct gttcttttgg gagacgtgtc gctgccgcca tcatcccgat caccctgccg
     9541 ctctcggcca cgataggcgt cccgaggaga tccaccgtaa acctcgtgcc gtccttccgg
     9601 aggccgacga gctcgccgtg ccaggctccg ccgccgatga ccgcccggat gatcatccgg
     9661 atcttgtcgg ggtgctccca gaaccctgag aagggcgatc cctgcacctc aactgccgcc
     9721 gggtagcccc agagggcgag gtacgcggcg tttgcgtagg tcaccacgcc atccggagca
     9781 aggagagcga ccgcccgccg tgtggaggct atcgcccggt ctttgatgag gagctcctgc
     9841 tcctgtcgcc gcaggtcggc gatatccgcg acgaggaggc ggtaggcccg ggcctcttcg
     9901 gtgatgtcct cgcaggtgac gtactccgtc ccgtcgggga gggtgacggc tcgggagagg
     9961 atcgtcttct tcgccccgtc ccgggatcgg actacaaacg tccggggtct gatctggccc
    10021 ggggccgacc gctcaaggtc cgatcgccag gccgcgacgg cttcctgcca gtcgctgtcg
    10081 tccgcgaaga tctgccggac ccattcggtg gccgtgaccg ccctgcccgt ctcgtagccg
    10141 aagatctcag agaatctccg gttgcagtaa cagaatcgcc tctcgtgatc gatgagcgcg
    10201 atcccgaagg gggatgcatc aagcatgctc cggaaccgca ccccgctcaa ccggagccgt
    10261 tcctcggtct cgcgccgggc aatagcgatg gagacctggc ggatgaagga ctcgatgatc
    10321 tctttgttct cgacctccct tccgggaggg aggaagatac ctgtgatgcc gtagagacgg
    10381 tcccgccaga ccagccctgt gaggcagatc ttcacgatat tgaaccgttc aaggatcgcc
    10441 tcgcagatct cctccgggat cgcccgaaag cagatgtcgt agaacggcca cgcggatggt
    10501 tcggggcgga gaacgaactc ctgcaggccg cgcatcgaga ggggggtctg gtgataggga
    10561 gcttcgaaca cgatggtgac cgggaggatc tggccgaccg ggttccgtcc aaggagttcc
    10621 gtgagcccct cccggaaccc ctcgcccgcg gtcgcccgga tagcgaactg gtgttcggcc
    10681 tcgctgtagg agaggatgta ggcgtacgct cccggcacga gctctgcgag gcggtcggcg
    10741 atgtactggt agatgtcctc caccggttgc aggtcaacga gatctatcgc tgtccgggcg
    10801 aggaactccg tattcctgat gtatcgcttc cgctcggtga tatcctcgag gatgagggtg
    10861 caccctctct ccccgctctc aaacgtcgtc gggatcgcct gcatctggta gaaggactcg
    10921 ctgccgtcat tccgccggtg ggggatatcg gcgatcacct gctcccgctc gaggttctcg
    10981 acgagggcga gcgatttcag cgaagaaacg accggaaggg ccgcttcccg gaggttccgg
    11041 cccacgagtt cctccttcgt acgcccaaac cgtctcaggc actggtcgtt tgcctggacg
    11101 atcgtgaggt cggcgtcgag gaccaggatc aggtttttcg tgaaacagag aaacgctgag
    11161 aggggtacgc gttgcgcgac agaatagacc tttgcgttgc cgacaagccg ggcctcgacc
    11221 tgacctgccg cctgcatcac ctcaaggtgc tttgagaccg agttccggtt tgatttgagg
    11281 gtctttgcga tctcggtgat cgtcatgccc ttgggccgga acttgaggct ccgcaggatc
    11341 cttcccggga tgtcctgctc agattgcata caaacaatga cagctaacac cgcattatag
    11401 catatatatt ttcctcacat tcccggggcc gccccccgcc cccgggacgc ccaccggcga
    11461 tggatggcgg agcttccggc aggggatggt cgccggtgtg ccggcagggt cgggcagagc
    11521 ccacggcttt aaggggggac gacatatcct gtacgaaagg cccggaggac accgaccatg
    11581 gaggagcatg agctcgtcag cataacccag ggagggttcg cgatcatgaa cccgaccacg
    11641 cccgagaagg tgctcgccgc cggacgggcg gcggggctcc ggaaggggtc ccgcgttgtg
    11701 gagatcgggt gcggaaacgg gacgatcctc gcgctctggg ggcgcgagta cggtgtatcc
    11761 ggtctcggga tcgagtcgcg ggaggacgcc tgccgccggg cggaggagac gttccgggcg
    11821 gcgggaccgg gcggccggct ccgcgtccgg tgcatggatg cccgggagta cgtctccgac
    11881 gaaccgttcg actgcgccgc atcgatcggg gcatcccaca ttcacggggg gtttgcagcg
    11941 acgctcgacg ccctcctcga cctggtccac gacgaaggat cggtcgtcat cggcgatcgt
    12001 ttctggcgat ccgaccgtat cccgcctgaa tttgcccgcg catggcccga cgtcccgacc
    12061 ggctacgagg tcctcggcac cgcccgggag gccggtctcg atctcgccgc cgtcatccgg
    12121 gccgacgggg ctgactggga ccgctacgag tcgggaatat ggcagtcggt cctctcctgg
    12181 cttggcgctc accccgatca tcccgaccgc gactccatga tcgactacct ccaccgtctc
    12241 caggacgagt acttcggcta cggccgggag tacatggact gggcgctctt cgtcatggtg
    12301 cccgggttct ggtgagcgga caaaaaaaag atggttccgg ttacgcaccg gcgtatacgc
    12361 ctgagccgac gaaccactcc ccgtccaccg gagcgacgta gcagagtttc agcccgtcaa
    12421 ccccggtatc ggggttatag taggtgatgt agacgaaccc gcccccgctc tctgccgcgg
    12481 cgctctcgag caggattatc ctgacaccgt accggtcttc gaggtcgaag cggttctccc
    12541 cgatgtattc cggctggtag gggagggcga gcgtcgtccc gttcatcgtg taggcgaata
    12601 tgtacgcttc tccgtcagca aaatccccgg agacatcgtt gaacgcggca cacgccgccg
    12661 ctcttccctg cttctgcgca tagtcccgtg cggccttcac tcgctggacg agcgtctccc
    12721 gaacgctcgt gttgaatgtg gccgcgacgc cggggatgta gacccctgag ccgacgaacc
    12781 aggtctcgtc cacggggagg acgtaaccga gtttgaggtc ttcggcgtac cctcgcgcag
    12841 ggtttggata gatatagttg atgctgccgc cgccttccgc cgcaagtctt ttcatgccgg
    12901 cgatgtaggc cacgccgctt acatcgtgca ccgcgctccg gtccgccccg atgagcgcct
    12961 gctggaaggg aagggcgagg acggtgccgt tcatatcgta ggcgaatatg tagagctcgt
    13021 ccctgacgaa ctccccggcg gggttgttga atgcagcaag tgcgtcggtg cgaccgtgct
    13081 cacgcgcgta cgcggccgcc tcgccgacga aagccttcat atccccggcg gtatcctcaa
    13141 cgggcggcct ctcgccggtt gtggccggca cctcaccggc atcggcgctc cgcgtggcga
    13201 cgacccgcca ctcggtcccg ctgatcccgg cggtatccca gatcgccttt ttggtcacgt
    13261 ttcgttccca gttccgttcc cagaacgagt actcaagcga cccggagggc tcggcgacta
    13321 tccgggagaa cgcctcctgg agtccggggg cctggtatac cggatctgca aagaggttac
    13381 gcccgatctc ctcaggtgtc gtatcgtaga ccaccagccc gtcggtctgc gtcacccaga
    13441 tgtcgtaggg ggtgccgttt atcaggggta cgatcgcccg gccgatgagg gcatcggagc
    13501 ggtacgcgac gctcacgaac ccgatgtact cccctcctgc tccaaagatc gggtaactct
    13561 gggcaacccc ggcatacccc tctgcaaggg gaaagacctc acttacgagc ggcacctgct
    13621 cttgaaccga tcgctcggtc tccgcatggg acgatatatc gctccccacc gtgccggcgt
    13681 agctctcggg cacggccatg gtcacgatgc cctctttcgt cgccaccagg gtcgactctg
    13741 tgtaagggtg gttcagcatc gcccgctgca ccgcctcctt gccggcggcg cttctaagcc
    13801 cggtctcccc gaggacccgg gcgctctcgc cgatcgaggc gttgatcgat tcgagctcgc
    13861 cgtttatcct ggcgacggct tccagaagaa ccgatttttc ggcctcttcc ggcggtggct
    13921 gactctctcc cacgcaccct gccccgagca ccacgacggc gacaaggagg atggcaatgc
    13981 atgcaggtgt tcgcataatc gcagttaccc cggggcaata ttaagtatgc tggtttttcg
    14041 cgatgctctt tgtgaaaggg cctcacctgt ggtcacgcca cgcttgagga tggcccgggt
    14101 gatgctgtgc cccgggtggg ggacgcccgg ctgacagggg gaaacaatcc gctctccttt
    14161 ataccgccgg tttcccgttt gtttcgtcat cccgggccgc ggggcaacaa cggtttcgga
    14221 agatgtggcg ccgttcaaaa ttctttcggc aaaatacggc ggaaatgagg gacaatagcc
    14281 gatacaaaat agaatcgcat aaataccgca tgcgtcatct aatgtttggg gaactactac
    14341 cccggtgaca tgagagaact gtagacgcga gtggagcgta tcccggaggg ttgcgctcgc
    14401 actcacccgt cgcgtgcggt gtttgccgcg ctcgggaggt cgggtgggca tccacctcct
    14461 gcacctcctt ctcgcgccgt gcgctctctt cttcgctccg ataagcacag cctgacctca
    14521 tcgcccacga gacggagggg aatgccccgg ttccgcgcga tcgccgcaag atcgtcatac
    14581 tcgggtttct ctgcaaccgg gcggccgttc gcgctcgcgg tcttcacgcg gatggggtac
    14641 tccctgccgc cgacgctgac cggcaccgtc tctttcctcc ggtccgcgac caccttccgg
    14701 aactcgcgca ccctgacccc gagggtcccg gtctcttcca tgagtatccg gatcaagcgg
    14761 ttttcatccg ccgccgcggc catcaccgat acgacgctga ccggccggtt cttcttcccg
    14821 agcgccggag tgacgaagac gtccgctgct ccctctgcaa agaggcgctc gatcgtgtag
    14881 ccgatcacct cgccggaagt gtcgtcgagg tttgtctcga gcatgacgac ccgatcctcc
    14941 cccgggggaa ggctctctcc ctccatcacc cggagaaggc cggtcggctc tccagagggc
    15001 cggatgccgt agccgacccg ggcgggggtc atggccgggt aaaggcttat ggagcgctcc
    15061 gtgatggcgg cgagcagggc tgccccgacc ggcgtggtca ggtccgcttc ggcgggcacg
    15121 gaggcgaagg ggaaccggtg ccgggtgaga accgcaagcg tcgtcggtgc gggtccgggt
    15181 atatcgccgc agtccgtcct cgttatcgtg gcgccgagcg ccggaggcat cgcgacgatc
    15241 gttccctcaa agaattctgc ccggtcgagg aggaacatcg tcccgacgac ggagaagatc
    15301 gtctccgccg gcctgaagct gacggcggta aggtcgccga ggaccgaaga tacccgatcc
    15361 ctggcgcccg gcgagagccc cacatcatcc gcgactgcca tgcacgcctc ctggaagtcg
    15421 tctccggcca ttgcgccctc ttcccgggcc gtgatcgaaa gaagggtcgc cgcgacctcc
    15481 tcgccggcac ccaccctctc tgcttcatag cggaactccc ggcagatccc gaggtcccgg
    15541 atggcgcccg caagcgggtc gagcgcccgg ggttccccgg tgaggtcgat gagggatgca
    15601 agaagtatgc tcccatcgac ccccgagcat cccggatcga cgataaggat cttcatggtt
    15661 ggcggtcctc acgccccttc gtccggtttg ccgaggtttg cgacgatatc ctgtgcgatc
    15721 gtcgccaccc gctccgccac cgcccggccg ccggggatgg cgttcgtaag gcgtgcgcct
    15781 ttcgccgccc ctgttgccgc ttctgccggg ctggtgccga gcgcggcagc aacggcggcg
    15841 ggcacggtga tgttgaggat cgtgttcccg ccgtactgca gctcctgggt gaggacggcc
    15901 cccgcgaata tggcagtgcc catgcccggg tcatcccgcc ccttcccctc aagcgcatat
    15961 tccgggatca cccgggcggt gaggttctcg atatggtaac tctctccgtc gatcctgaca
    16021 tcataggaga ccttcgcatc aaacccccag aaccgcgcgg tgagcgggtc ggtcctccgc
    16081 gcgtggggcg caatctccgt gaaccggaag gtgatctcct ttccggcgaa tcccgagagg
    16141 atcgcagaac cccgcctctc aatgagagcc ttcttctcct catcgagggc tccggcagct
    16201 tcttcaagcg tccttccttc tttgagcatc tcatggacct tcaccgcccg ccggtagatt
    16261 gcccggttcc gggtcggttc gctcgcccgg atgcaggcat ccgtcaccgg accacggttg
    16321 atgacctccg cctttcgggt gatggtattc gtggcgcaga gggcggtctc ggggtcgatg
    16381 aaggactgca tcttgtagtc ccgtatcgcc gccgcggtac gttcgatgtc gccgccgttc
    16441 ccgatcagca gccggagcgc cggcagtata tctccggcga ggtgaccgag ggggacgttc
    16501 accggaccac cggagaaccc ggctccaatg gcggcggatt cctggaatcc ggcgaagagt
    16561 tcgttcgtga ccatcgatcc gatcaccgac ggtccgccga cgtctttcaa gatcaatccg
    16621 aagtcgccga cgagccgggc ggtctcgtac tcctccccgg tcccccgcat atggatcttc
    16681 ttcgggagcc ccgctgcctc gacggccccc tttccggcaa ggtaagagga gtcgacctcg
    16741 ccgaacttcc cgaactcttc cccgagcagg gcatccgggt ggacgagctc catcgtcacc
    16801 gccgccgcga tcagggcggg ccagagggga taaggggtcg tcccggctcc ctccatgctc
    16861 cgcatcagcg ccctggtccc gaccatcgcg gcatttctcg cgagttcggg tatgttgtag
    16921 tcctccccga gggcggcatg tcccatgacc ccgccgccgg cgacgaacgg cggcaggacc
    16981 gcgccgtcga ggcgggtcaa ccgttttccc atgagggcct cgtatatcgc gaggtaggcg
    17041 gggaacgcag ggacccggtg ggtgaacttg ttcgtcgtca gggcgatggc gcttgtccgg
    17101 tcgccgccgg catgcatccg ggcgatggcg ccgagcttcc ggttcccgag cgggacgccg
    17161 gcacgggcgc agctccccga gaagtaggcg agcgtggcaa cgataagcgc cgcgttcgcc
    17221 ggttcggctc caccccgctt tgcctcaccc accgccttcg cgatgacgga atcaagcggc
    17281 aggctggggg ccgaagcatc gacgaccgcc atatccatga ggcgcatctc cggggcgccg
    17341 atgctgcaga agatatcccc cgcaagcgag ttcgccgctg caaacgccgg gataacgagc
    17401 gccccgtgcc cccgggtcgc cgcctccagc ttgtcgacgg tcatgtagtc gggctcatac
    17461 gtcacggcat gggccgcagc gaggagcacc cgctctcgtt ctatctccat gggtccggta
    17521 acctcccttc ttttacgcgt gaacaggagg tttcgggcat aaaaaggtgc cgcccggggc
    17581 ggtgccgggg gatccggcta catgtacagc gcctggatcg cgacgacttc agcgctgtct
    17641 tttgcctgtg cgtagtcttc caggggctcg gcgttcacct cgaggaaccg cagcccccac
    17701 ccgaacgggg cgaggatggt ccgggcctgc tcctcctcgc cgagtatgga gagcgtcgcg
    17761 gccatcgcct ccaccgatgt cagccggaac ggccggccga agttcaccgg gtttgcggcg
    17821 acgaggaagg ggagcgcccg gcggttgcgc caggacgcga cggcaccggt atcgaggacc
    17881 tcccaggagc agtcgagggc cgttatcgag gggagatggc ggtcggcggg ggagaccgcc
    17941 cgctccgcgg tcgggtcgag gaggagggtc gaacgaggga tctttgcgat gttcgtgacg
    18001 atcgtggcaa gcccgcgccg tgcgagcttc tttaccgtgc acttccgggg gtcgcaggtg
    18061 ttgtcccggt aggcgtagag cggtatcatc tgtcttgtta tggtacgccg ccgagatctt
    18121 atcaatgtat gtactcatcg ctccctgcat cgagaacccc gcctgccgtg cccgcggtat
    18181 aacgaccgat gaggatctcc gccacttcat ccgggccgtc gaacgctgcc ggcacttctc
    18241 gatcgatatg gtcccgctcc cctgcccgga gacgatctac atcggggcgg atcggccgcc
    18301 gggttcgtac ctcgaccgcc tcgcgacccc cgcgtttgcc gctctcctcg accggctcga
    18361 ggcggaggtc cgcgccgtca tccgcgagcg gggcgaggcg cctcttgctc tcgtcggtgt
    18421 ggactcctcc cctgcctgcg gggtgacggc cacctactat tcatccgtaa aacagcccgg
    18481 gcgcggcgcg ttcctcgccc ggttcccgga tataccggcc atcgacgtga aggagttcgc
    18541 ccgttaccgg atctacctcg caggccccct cttctccgag gctgaacggg cctacaacct
    18601 catcctccac gatctcctcg cagtgcacct cttcgacgtc tatcttcccc aggaggccgg
    18661 ggatacggac cccgcccggt gcggcgagga gcatggggcc atatttgcac agaacctcct
    18721 ggctctccgc gacgccgatg tcgtcgtcgc ggtcatcgat ggcgcggatg ccgactccgg
    18781 gacatcctgg gagatggggt atgcctatgc cctgaagaag aagattgtcg ctctccggac
    18841 cgatttccgg gtcgtcggcc accacgagcg ggtcaacctg atgctcgagg agtcggccgc
    18901 ggtcgtcacg aagaaggagg atcttccccg ggcgctcggg tcgcttctcc cggcgtcacc
    18961 ctgacgccgg ccgcctcttc tccatcgaga ggacgtttct gtcgtccctg cgggcatacg
    19021 ccacgctatc catcaccgtc cggatgaagt ggataccaag gcccccgatc ggccgctctt
    19081 ccgcggggat atcaaggagc ggcggcgggg cggtcagagg atcgaacgca acgccgtcgt
    19141 cggtgatcgt cacgcggatg acgtcgtcgt ccacggtgca ggcgatcgtg atcgctcctg
    19201 cctcccggtt cgggtagccg tagaggatgg tgttgctgca ggcctcgtcc acggcaagct
    19261 ggaccgaaaa gaccatatcg tcggcgctcc cggcgagtgt tccggcaagg aaatcggcga
    19321 tcgccgtgag agccccgagc tctgcccgca ccgtgagttc cgccatcaca ccacccggag
    19381 caccatgagc gtctggtcgt caaactgcgg ttcgtccccc gagaaatcag cgaccgcccg
    19441 gcggatacga tcgacgatct cccttgaggg gaggtcgtgg cagcccgtga cggtctcgat
    19501 cagtcgctgc tcaccaaact gcttctcatg ggagtcgatc gcctcggtga ccccgtcggt
    19561 gtagaggagg acgaggtcgt tcttctcaag cgcgagggtc tcctccccgt agtcggcatc
    19621 cggcatgaca ccgaggatga taccggtccc tttcagctcg agcacccgtt cgctgcctga
    19681 ccggaagagg aggggagggt tatgccccgc gttgacgtag gtaagcgtct tcttcaccgg
    19741 atcgacgacc gcgtagaaga gggtgacgaa catgccggac tttgcgtctg cggcgatcag
    19801 ggcgttcgct tcgcggacgg catcacgggc gctccgtggg atcagagcgt tcgcccggag
    19861 caccgtccgc gagagcgcca taaagagcgc cgcgggaacg cccttccccg cgacgtcggc
    19921 gatgacgaag gcaattcgct gcccggagag ggggattgcg tcatagaagt ccccgccgac
    19981 ctcttttgcc gggagcgaga aggcggcgat atcgaggccc gggacatcgg gcagccgttc
    20041 ggggaggaag ctcatctgga tatcgtgcgc aatccggagc tcggccttct tccgctcgag
    20101 ttccgagagg taagcatccc gctccgcttt cgtctgccgt tcagtgatca ggttcccgat
    20161 gatgagcgcg aatatgaaga ccccgatggc gtttgcgatg atcatcggaa gggccacctc
    20221 tctgacgacg gtgagcgcct ggtcgaacgg ccggcagagg aggagggtga tgcccatgtg
    20281 aaacgcctcc atgccgatgg caaagaggac cgcgccgtgt atcccgacga acttccgtcc
    20341 ggagaggagg aagatcaacc ccccgaagag gcccgcaagg atggttgctg ccgagcacgg
    20401 gacggcggtg aaccccgcaa gggagaagcg gtaagcggcg ccgatcaggc cggcgccgag
    20461 ccccaccacc gggccgcacg cgagcccccc gaccatgggg ccgaggtcgc ggacgtttgc
    20521 agtggcgccg aggatggtga tgccgctctc ggtgccgtag atggagagcg ccccaaagag
    20581 gaggatgagg atcgcctggc tcttccaggt cacgaggccg tcgagcaccc gggtaaacga
    20641 cgctgtccgg gtgatcaggt aggcggccac caggatgacg cagatcatct ggaagagcac
    20701 aaaaaagaga tccccaaatt cggagaacgc catattgttc agggccccgc cgggaacccg
    20761 gcaagcgccg tcgcctcgtc gggggagagg gagaagatcc ggtgaaagcc cgccatcgtg
    20821 aagacctcaa gtatctgcgg ctgtgcacag gctatcctga gcgctccgcc atctttcttc
    20881 agacgtttca gtgcggcaag cagtacccgg aggccggagc tgctgatgta gtcgagatcg
    20941 cagaggttga tgaggagata tctccctccg gcatcgagca ccctgttgat ccgttcttcc
    21001 gctttctccg aagagcctgc gtcgagcctt cctttcaccg caatgatgtc gacggcgccc
    21061 gctctccttt cgagtatatc aacacgctcg ctcattgaat ggatattcga tctggtgagg
    21121 gatagatgtt actctctgtt ccgggacctt ctgaggggga agcaaaaaaa atgagggtgt
    21181 attgtcgggt gtgtgataac catacggggt atttgggcag tcccggtgcg gttgtgccgt
    21241 ccgcgccggt gtcctgttcc accgtgccgg tatgagtgtt cagagaggtg tgcctgatca
    21301 tattcaccgg ggtgctcccg gcgtggacgt tggttctgac gtcggtgcaa tcagcgcggt
    21361 gaatgttccg ttcaccgacc ccagccgttc ttgtgccggt ccggaccacc tgtgcccgtg
    21421 tggcccgggt cgccggcaga gtttcccctt ctctgccgac tctggggatc atgcattgat
    21481 caatagagta tatatacttt ctctttaatg ccttgcattg tggtgtggca ttgcatagag
    21541 catcatgaca tgcagcactg caaaacacta tatcgtccgg cgcgaaactc tgttcgcaca
    21601 tggatcccac taccggcgca ctctctctcc tgctcagcaa tttaaaagag catccgcggg
    21661 gcatgtcggt ctccgatctt gctgccgccg ttggaatcaa ccggaacacc gtctcccggt
    21721 acctcgacgt cctcctcgtc tcgggccagg tggagatgga gacctacggg aaggccaagg
    21781 tcttctacct ctcgcaacgc gtccccatcg ccgccatgct caacttctca tccgatctcg
    21841 tgctgagcct cgaccgcgag ctccggattg tccaggcaaa cgatgccgtt tgcgcgtttg
    21901 cgggaaggga gcgggatgat atcatcggga acggcatcga ggcatcaccg cttgcggcgt
    21961 tcgaccaccc gctgatacgg ggccggatca ccgatgccct gaacggccgc gaggtcaccg
    22021 aggagctccg cttcctgcgg cacgaccggg aactcttctt ccgtgccaag ttcctcccca
    22081 ccgtcttcaa cgacggggcg ccgggagtca ccatcatact cgaggatatc accgaggagc
    22141 gccgggcgga agaggccctg cgcgagagcg aactgctctt ccggagcctc gtcgagaaca
    22201 tcagcgacct gatcctgaac gtcgacgaga cctttcggtt cacctacgtc agcccgaaaa
    22261 gccgggagat ccttggttat gcgcccgagg agatgcttca caagactccc tgcgacttca
    22321 tggcccctga gaatgccgaa cgggccaggg cacagttctc cgcgctgctc gccgacccgg
    22381 agccccggat tctctttgag tggacgatgc tccatcggga cgaaagcgac gtcatcctcg
    22441 acgtgagcgg gacgccggtc tacgatgtga tcggcgactt caccggctac cgggtggtct
    22501 gccgcgacgt caccgagcgg gcgcgtgccg caaagcgggt ggcccagtgg aagtcgttcc
    22561 tctactcggt cgtcaacaac gtcccgggca tggtcttcgt ccgggaggtg gaagggaaca
    22621 cgttcgtctt cgcaaaccgg gctgcggaga tcttccttgg catgacgcag gaggagatgg
    22681 cgggcaggca ggccgcggat atattcccgc cggagatggc cgtcttcttt gccgatggcg
    22741 accgggaact cgtggagcgg gcggccgccc ttgagaagga gatccggata tcaggcgggc
    22801 ggacgctttc catgaagaag atcccgatat tcaactcccg gggcgtgctc cgctacatgc
    22861 tcgggatcgc cgaggatatc accgaccgct ccgctgccaa ggatcttctg gtcgccgaac
    22921 gcgaccgggc gcagggctac ctggacgcgg ccggcgtgat gatcgcggtg atcggggcgg
    22981 acgggaccat cgatctcatc aaccggcggg gaagcgagct cctcgggtat gccggggacg
    23041 atctcattgg aaagaactgg tttgcgacgg tcgtcccgga gcgcctgcgc gatccgctcg
    23101 cccggaactt cgcccgcctc gtggccggca ggatcgagcc gcccgccttc gaagagagcc
    23161 cggtcgtcac ccgggacggc caggaacggt tgatccgctg gcacaacgct ctcctccggg
    23221 atgcagatgg gaaggtcgtg gcgatggtga gctccgggga ggagcccgcg gggtagctgg
    23281 atcctccctg ccggtatggg aaatccggat ggattcggcc atacccgcgc atttgagggt
    23341 tatctcttaa atacgcctta tagtacatac actatatggt gacaactttg gtggacatca
    23401 tctcccttgt ccttatcgcc gtcgtggtgc tgatcgtcat cggcatcgtg gcggtcatga
    23461 taggcatcta caaccgattc ttctccctca aaaactcggc cgacgcgacg gtcgggcagg
    23521 tgaaggtcgc catgaagaag cgactggata tgatcgagca gctcctcggc gcggtgaaga
    23581 gctacgcggc gtttgagaag gataccctga ccggggtgac cgcgatgcgg gcccgcatcg
    23641 ggagcgccgg ccccggcgac ttaaacgagc ttgagcgcga gtcgcggtcg gtcctcggcc
    23701 ggctgattgc cgtcgcggag aactacccca acctcaagac atctcagacg gtgcaggacc
    23761 tgatgggcgc ggtgaaggga atcgaggatg agatcgcccg gcaccgttac acctataaca
    23821 acatcgccca gcaatacaac accatgaccg acacgatccc ttcaaacctg gttgcgagcg
    23881 tcatgggctt taagaaactg gaatacctgg agttcggcga ggatatcgag cgggttccga
    23941 cgatagcgtt ttgatagatc atgcgcgtga ctgaacagca gcagatcctc atccttgttg
    24001 ccatcaccct gcttatcggg gtcctggcgg tcgccggagc tgtcgcgctc cccggtctcc
    24061 tccagggaga cctggacgtc gggcactacg acgccgtctt ctacgagaac gggaccctca
    24121 tcgagcgcta cacctacgat gtccgcgccg caggcgagta ccggatgctc tttcgctact
    24181 gggatgcgcc gctcaccttt gcggccatcg accggccgca cgtagagttc ctcggtatga
    24241 ccgcaccacc cggggtcatc ggctacgcca gggattccgg aggcgaggtc cggacggccg
    24301 ccgggagtgc gaccccatcg gatcttgcca cgatccggaa ccttgcactc gatagcgaag
    24361 tgggtatctt caaccccggc tactttgccc cgggcaccta cacggtggaa taccgttacc
    24421 tggtccggcc gccggtcgag tacgacagcc agtgggcgca cctgaacctg aaactcgtgg
    24481 acgagcacgt ccccttccgg aacctccgga tcaccctccc gtttgccggg gatatcgagg
    24541 aggtctacac gcacccccct acccttgata tcgagcgaac ggcggacgcc gtcgtcataa
    24601 cggggagcgc atcccagaac gacgcgctga acgtcgaact gatcctcgtt ccggcgttca
    24661 ccgagaaggt cggcggattt ggggagttcg tccccgacgt gcggcagaag gcggccgacg
    24721 cgaatcgctg gccccccctc ctctacgggg ccgcgaatat cctttacggc ctctcgatcc
    24781 tcctcgtcct cgcgacgccg ttcatcctcc ttggcgtcta cctccgctac ggcagagaga
    24841 aaccgttcac ggtgccggaa tacctgagtt tcacgccgaa taccgcgctc aaaccctggc
    24901 aggtgaacct cctcttcaag ggcgacgccc ttgagttcga cgataacggg ttctacgcga
    24961 cggtcctcga cctccaccgg cagaagaaga tcgtcgtgac cgaaaaatcg ggcggcaacg
    25021 gtgtcacggt ccggatcgtc tcgggggagt cctccgaccc ctacgagcag cgggtgctca
    25081 acttccttgc caacatagcc gacgaccatg tcgtggatac cgccgatctc gagacgttcg
    25141 ccgagaccgc ccgacggagc ccgggttacc agcaccggat cctgcagtac cagcagtcgc
    25201 ttgccgctct cacccggaat gtggatacga cgcttgcccg gcagtatgtc agtgatggga
    25261 ggggactcat cctcccgctc atcttccttg gcgccatccc ctgcgggctt gcgatcctca
    25321 cgatcatatt cgcgccgggc gcggcctacc tcctcatacc ggccgggatc ctctctttca
    25381 tcgtcgcggt ccaggtgggg atagcggccc tcttcccctc gaccctcttt ggggtctgga
    25441 agggcgatcg ctaccgggag aagctcgagt gggatgcgtt tgctcacttc ctctcggacc
    25501 tcgctctgat ccggcagtac tcccctgccg acctctcgat gtggggggag tggctggtct
    25561 acgggaccgc gctcggcctc ggcgataagg tcgagcaggc gatgaagaac ttaaacatca
    25621 gcctcccgga cgtcgggatg ccgctctact cgaacatgcc ggtgatcttt gccccgatcg
    25681 tcttctactc gccgccgtcg tcgggcggtg gccatggtgg attcgggggc ggcggatcct
    25741 tcggtggcgg cggtgggttt ggcggcggcg gcgtcggggg acgataatac ccttttttct
    25801 gccccgccgg ccggataggt ccgaatctgt tcgtagttaa ggtaatctac ttacatccgg
    25861 cgcgagaatg tacgggtagt gatgcctatg aggggatcct atctgaacta ttcagccgtc
    25921 gtcctcgcgc tcctttcagt cgccgtcgcc tgcgggtgca tggccggcgg ggagaaggac
    25981 gatgctcgcg accgcatatc cggaaacggg acgatcacgt atctcgatct cgagggcggt
    26041 ttctacggta tcgtcgccga cgacgggagc cgttatctcc ccgccgacct tccggcggac
    26101 ttccggcagg atggcctccg ggtcgcgttc gtcgttgata gagcagagga gacagcgacc
    26161 atccagcagt ggggaacgcc ggtcgatatc gtcagcatgg agaagggcga cgcccgccgg
    26221 acggtcacgg cgaacggaac ggtcacctac atcgatctcg agggcgggtt ttacggtatc
    26281 atcgcggagg acggcatgaa ctgtcttcct gcgaacctcc cggaggagta ccggatcgac
    26341 ggtctttctg tgcggttcag cgccgacctg gaggaagatg tcgtggggtt ccctatgtgg
    26401 gggatacccg tgaatatccg gaccatcgaa ccctccggcg gtgtccgtct tgtcgccggg
    26461 aacgggacga tcacctacgt cgatctcgag ggtgggtttt acgggatcgt cgcggatgac
    26521 ggcgaacagt atttcccgct cggcctcagt gaagcctggc tggtcgacgg catggacgtg
    26581 gcgttcgttg cccgggcaag ggaggatgtc gcggccatcg ggcagtgggg ggctccggtc
    26641 gatgtcatcg ccatcgataa ggcggggagc gcaacgtttg tcgctgaaaa cggcacggtc
    26701 acgtatatcg acctcgaggg cgggttctat gggatcatcg ccgatggcgg ccggcactac
    26761 ctcccgctcg ggcttgagga gcgctatcgc gtcgacggca tgcgggtcgc ctttgcgggg
    26821 aagatcgcgc gcgacatcgt gaccatccag cagtggggaa caccggtgga gatcctcgcc
    26881 gtcccgtggg cctgctcatc ctgtggcggg agcgccggca tcgcaaaccc tgccgcggcc
    26941 tggtgcctcg cgcagggcca cgcctacgag atccggaaga accccgacgg cagcgagtac
    27001 ggcgtctgca tctttgcaaa cggcaccgtg atcgatgagt gggactatta ccggcagagc
    27061 cactgaggct ctctacaggg ttctccccct ccctctcttt gcgcgaggcc gtttcatcgc
    27121 tcataccctc gagatcccgc gttgccgcga agggtgcgaa gtcctgcccg ggcacctgca
    27181 cccccccttc gcgcgatccc gtacgaccgt ccctctcccc ccggtctgag acgggcggct
    27241 ttttcccttc ccgcgccgaa aggagattat ggatgtaacg gtcagcgaac gtgacgatac
    27301 ggcaatcgtg tcggtcgacg gcaggatcga tgcagaatcg gcggaaaacc tggatacggc
    27361 gatatccggg gtgctggccc ggggagggcg gcgggtgctc gtgaacctgg agaaggcggc
    27421 ctacatgagc agcagcggtt tgcgggtgct gctcggcaag cttcgtgccc tgaagggagt
    27481 tgatggggag atggcgctct gctcgctcca gcccagcgtc taccaggtct tcttgctcgc
    27541 aggggtgcac gaagtcttcc cgatctacgt gggcctcgac gagatcctcg tcgggaccga
    27601 ggatgaggag aaggagtcgc cgtgacggag ggcctgatcc gtatcctccg tcacggcggg
    27661 agtgccgacc ggatggcggc ggcggaggcc ctcgccgcgc tcggcccggc cgccctcccg
    27721 gcgcttcttt cggcacttgc cgaccccgat ccccgcctca ggatgtg
//
LOCUS       DAOSII010000009        27763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_1921_length_27763_cov_49.006244, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000009 DAOSII010000000
VERSION     DAOSII010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 27763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27763
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1921_length_27763_cov_49.006244"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="PLV90_01405"
     CDS             complement(<1..668)
                     /locus_tag="PLV90_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOF95800.1"
                     /translation="MRYYPVHSGYPPYTRVNQAIEAAFAEHGRGGVRMPSKVYVGLVE
                     NGDFRTMPAYMPALGIAGVKIVNVHPNNRARGLPTVMALTEIIDVTTGVPQAIVNATE
                     LTAMRTGAAGAIAAKYLSPRRTVTLGVVGAGRQAEALVEAIAATLTIDEIRVWSRTEK
                     SSEAFAARYADYNAVSVSIERACDCDVLNTTTPSRKPLIMADWIGEGTHINAIGADAP
                     GKQEL"
     gene            complement(817..1470)
                     /locus_tag="PLV90_01410"
     CDS             complement(817..1470)
                     /locus_tag="PLV90_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HOF95801.1"
                     /translation="MLPDGISLGRLQGPLSALASISPRFTGAVRIKGPGGTGFILAQG
                     GNPYAAAFEGTGGGLPLRGDEAYRYLLDRPSFDYELIAYTADETGAAREVAREAGCLI
                     SGDGKTPASRANSGETARLDRIARQPGVIAVSVFHEGFALQSLGSADFDQVAAVAEDL
                     LRAGVRITADMDMGDLAQVILETPAGKLIIAPYGDLSLCILAKPDANLGLIRLSIRG"
     gene            complement(1475..1822)
                     /locus_tag="PLV90_01415"
     CDS             complement(1475..1822)
                     /locus_tag="PLV90_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HOF95802.1"
                     /translation="MLRQILGEFLRLDGVTAAVVAGRDGFVIESVVAGDIDVEALGAM
                     ASTGMGTSEAMSNELGKGEMHQMLVELENGPILLSPLSEDELIAIVANANVNVGRIRY
                     ELKKNRDRITAAL"
     gene            complement(1834..3201)
                     /locus_tag="PLV90_01420"
     CDS             complement(1834..3201)
                     /locus_tag="PLV90_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOF95803.1"
                     /translation="MKEITISPVTRIEGHAGVRIYLNDRGEVDSAHFQVVELRGFEKF
                     LVGAAIEEAPRITPRICGICPSAHHLAAAKATDRIFGVTPPATGKNLRELLGLGQFVH
                     SHALHFFMLAAPDFILSPDAPPEERNVVGLARHSPDIARKAITVRKFGQRLTEAVGGK
                     PIHPANALPGGMSAPLTEGKRDELAAMADEALGIAREGWEIAKNLLDGVDTEIGAVRT
                     GFMGMTNHGLYSTYDGPVAMLDPDGGRIGSFSGQDYTNFVEEYSEDWSYLKFARLKGG
                     GYYRVGPLARLNIVERMGTEHADAALAEYRERFGTVTQAPLAYHLARYIEFLASCERA
                     KDLLADPGITGSGIRTPAGKVVNRRGVGIIEAPRGTLIHDYTVNDAGIIEKCNLIVAT
                     CQNNYAIDRGVEDMARRVVKNGTLTEGAANRIEMIIRAYDPCISCATHAIGRMPLRIE
                     CMRRT"
     gene            complement(3198..4115)
                     /locus_tag="PLV90_01425"
     CDS             complement(3198..4115)
                     /locus_tag="PLV90_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-nonreducing hydrogenase"
                     /protein_id="HOF95804.1"
                     /translation="MKIAIEELAGCSGCTIAVLDLHEMLLDVVAKADIVYSPVIMDVK
                     EPPEGIDIAFVTGAVRNEENRERLEKIRKRAKTLVALGTCACYGGISGLSMLSSNQDL
                     FSCVYRGVETAKPDGVIPTDVPPFLYRAFAVGDLVKIDYYVTGCPPKEAFLKKIIPAM
                     VAGDTLELSRKSVCSECDRTMGPVGDWTLRRRHEGVPDREHCLLGQGYLCLGSVTFGR
                     CGATCPKNNIPCHGCNGPSLDILREPCRDIYGMMVRRISDLTDKPAKEVEKELYDVAH
                     TMYAFTIGSLIMEDKETSKIRDLVKERSR"
     gene            complement(4126..4689)
                     /locus_tag="PLV90_01430"
     CDS             complement(4126..4689)
                     /locus_tag="PLV90_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrocarbon binding protein (contains V4R
                     domain)"
                     /protein_id="HOF95805.1"
                     /translation="MGAGYIDELHEAFHSDLVYRPEDIPIDCSPRPKDLEQTLHGVMK
                     LNGLVIRSLEEIAGRGANAVTYRAGKKFGHEVAKYFQKREDVEGALHELSDLLRGQYS
                     FEVWKPEDKETFIIEENGETFIYLVFHDCIVRQTLRRNGLDQGGPLCQTLFGYVVGAV
                     EEITGRKARLEIVHTGPNACLKKLVLK"
     gene            complement(4689..4946)
                     /locus_tag="PLV90_01435"
     CDS             complement(4689..4946)
                     /locus_tag="PLV90_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOF95806.1"
                     /translation="MQRLIMDIFVDKDACVGCGLCVKDCPMHVYELQNSVSVPVRPNN
                     CMGCLSCHEICPAQALEHRGIYPARRHYIDIKVCEMLRKVI"
     gene            complement(5392..6180)
                     /gene="minD"
                     /locus_tag="PLV90_01440"
     CDS             complement(5392..6180)
                     /gene="minD"
                     /locus_tag="PLV90_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATPase MinD"
                     /protein_id="HOF95807.1"
                     /translation="MVKVYTIASGKGGTGKTTVTANLGPMLARYGKKTCILDADVGMA
                     NLGLILGLEDLPVTLHEVLAGKARIRDAIYEGPYGVKVVPCGLSLQGFQQSKPERLKD
                     VMADLVSGFDVLILDAPAGISRDGVIPLTIADGVILVVTPEISSIVDSLKTKILTETV
                     GGHVEGAIINRVAGNGDEFNRAQMERLLGVRVLGIIPEDANVRRASAGRSPIVVKYPD
                     SEASRALKRISADVAGIEYTDESVPAKREGFIDRLARALFRAKA"
     gene            complement(6217..6717)
                     /locus_tag="PLV90_01445"
     CDS             complement(6217..6717)
                     /locus_tag="PLV90_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95808.1"
                     /translation="MDSTGIIQIFLVIMTVTVIAGFAGVLYYLSRIAQALAKEIPGGA
                     IESQERPAGNRESPGDIDPRGAKSLEENLGAISRKYSLASFTLATADGLLVGSTKTGS
                     EGEAARYSHLYARGERQDGTGAELLGIPYRGETLVGIARPKEHLSAELMNALEQDTRD
                     ALQRWV"
     gene            complement(6740..7375)
                     /locus_tag="PLV90_01450"
     CDS             complement(6740..7375)
                     /locus_tag="PLV90_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95809.1"
                     /translation="MQLPRGRFHRLIKSTTSRVLIEEMASARFTGICTIVPGEKSAVL
                     VLNEGLVVLAEYGGIRGQHALDAVSNDESEAAAELNLLTPEQVQLAREFNQPCAIDAG
                     DAGREKRTSPTKPAPGGGRPLAGVAEPTAACSPEHRAEPTGEGHRIPMPGTRPVQEMV
                     AATPSGNDEIDMLIQNMEKMDVEQLISNFRINCKEMLRNIHLDHLIQDGDI"
     gene            complement(7462..8007)
                     /locus_tag="PLV90_01455"
     CDS             complement(7462..8007)
                     /locus_tag="PLV90_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOF95810.1"
                     /translation="MRENRIGIIGCGNPFMGNDGAGILVMQLFEGRYPGVDAIDGGTG
                     GFGLIPLMEGYEKVVIVDAMTGIGNRIGDVVVFATPPSLDLPACSLHDIGIGDAVAVA
                     RELGCVAEIVTIGIEVGDIRAFHRGVDPAVEGGVRAAEKEVVRLLWDWMDEPCGTLAG
                     SKPGGGSESPSPGQGSSRVRL"
     gene            8103..8897
                     /locus_tag="PLV90_01460"
     CDS             8103..8897
                     /locus_tag="PLV90_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOF95811.1"
                     /translation="MIHIVPKPTDTPDDNSTGAVIRELERAGARCRVIDLGTIDPLDS
                     GLENELIWVCGIRQDGHQFETLSVLALHNQVVNTPESIVTCASKVMTTALLLHDGIRT
                     PETAYMRSEAQAGAFLKRHGKVVYKPLYGFDGNGIRLVERPDDFGPGPWYLQEYVPND
                     RDFRVFVIGGEAVGAIARVSEGLMHNIHQGGIGSPVPIDEEMRAIAEASARAVDIDYC
                     GVDLLRDKEGYTVLEVNGTPNWHCMAAPIPRLLAAYLIERRRAMRG"
     gene            complement(8967..9251)
                     /locus_tag="PLV90_01465"
     CDS             complement(8967..9251)
                     /locus_tag="PLV90_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0058 family protein"
                     /protein_id="HOF95812.1"
                     /translation="MHKEELIALHGILTEIKDYFELMNPELKFSQYYALKIDPSQVHK
                     SKMEHKYAIFVLGTELANAMKDVEFSSSGRISARMKELAEKALKEIEYLQ"
     gene            complement(9315..10544)
                     /gene="dnaG"
                     /locus_tag="PLV90_01470"
     CDS             complement(9315..10544)
                     /gene="dnaG"
                     /locus_tag="PLV90_01470"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase DnaG"
                     /protein_id="HOF95813.1"
                     /translation="MYSPETTKYLIHLTLQAEGVVDKPDVVGAIFGQTEGLLGEDLDL
                     RDLQRTGRVGRIDVQITTKRGETKGEILISSSLDRAETALLASSLETIDRVGPCTARV
                     KVDRIEDIRVTKRRKIVERAKELLLEDFDEGAISSDDLLDEVREVIRIEKLEYLGEER
                     VHAGPNVMASDAIILVEGRADVINLLRYGIKNAVAVEGTNVPRVIIDLCSQKTATTLL
                     DGDRGGELILRELLQVAEIDFVAYSPRGKSVEEMSRKEIVKALRNKVPVEVVTDHIVD
                     EGAAAERLPPPAGPAEARPPAGAGEGSSGERPAAKEGSRRPPSTLGEHMADVRDRKIA
                     RFLSPDYTVLAESNAADVEGALQTLAGDVEGLVVDRIVDQKLLDQIGGRDLEFVAARD
                     FKGIVKRPLSIRLIKIG"
     gene            complement(10787..11104)
                     /locus_tag="PLV90_01475"
     CDS             complement(10787..11104)
                     /locus_tag="PLV90_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOF95814.1"
                     /translation="MEPYADAVVETAHGVVITLDVTAGAKRSSFPAGYNEWRKSIRCQ
                     VAAPAVGGKANRAVVDLVAGTLGVPRADVVILSGHTSSSKTVAVAGRSRDGVLACLNA
                     AGA"
     gene            complement(11303..12565)
                     /gene="pyrC"
                     /locus_tag="PLV90_01480"
     CDS             complement(11303..12565)
                     /gene="pyrC"
                     /locus_tag="PLV90_01480"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOF95815.1"
                     /translation="MPPVTADLVLKNVLLPTGRSADIVIAGGVVRHVGGAVRADETID
                     CSGLTCLPAAVDMHVHMRGGVQAEKEDWRTGTESAVAGGVTVVVDQPNTIPPLTTPDL
                     LCARIREAEEHAVCGFAVNAGVTTDADLEGMRNAGAMAFGEIFAAPSSYGEGLDPETL
                     ERLLARIHAMGGLATVHAEEVAGPAPATLAEHDRARSGAGEARAVRAVRALAPGGMRL
                     HFCHMSTAASVEAARGTVEATPHHLFLSRDMFAEDDARARVNPPLRDEETRRGLWSCW
                     ERIDIVASDHAPHTLREKELPFEGAPSGMPGVETMVPLLMAAVRRGDIPLASVIEKTS
                     WRPAAILGIRRAGFLPGDRADFALYPDAVTRIDAGSLHAKCGWSPFEGLDAIFPEEVV
                     VRGRRTYAGGELLEAHPAWYPGQGYLSL"
     gene            complement(12553..13467)
                     /locus_tag="PLV90_01485"
     CDS             complement(12553..13467)
                     /locus_tag="PLV90_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOF95816.1"
                     /translation="MLEFSEFKPVSLDDRDLFLRQYRQYPQVHSDNTFANMVCWNHYA
                     NYCFADVDGAIVLSSTIDGVTSFRPPIGPKNPALLDDVIDLALREGGAAPLLVLDPVN
                     EAWIREIYPNLILYPDRDYFDYIYRTEDLADFAGKGYATVRRQVNHFRREYAYSVEAI
                     TRENIDEVWEFLVIWCEWRDCDSVPILAYEKEAILFAINNFFAIGLRGWIIRVGETIG
                     AISVVDEVNADMAVVHFEKALPETYRDIYKVIMTETAAGLRRRYPFINRECDMGVPGL
                     RESKTRCHPACMVEVHYATRDDLEACRR"
     gene            complement(13472..13903)
                     /locus_tag="PLV90_01490"
     CDS             complement(13472..13903)
                     /locus_tag="PLV90_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF95817.1"
                     /translation="MIEQRAIDVRLVSVWDGDMIADLYRAGGWWNEEWDPAGLRALIA
                     GSFAFAVAIDPAAKKAVGMGRVISDGVSDAYIQDLVVLPGYRGRGIGTMILSTLLDHC
                     KSAGVTWVALVAEPGTEAFYTPLGFRRMEGHTPMRWYPDER"
     gene            complement(13984..14979)
                     /gene="hypE"
                     /locus_tag="PLV90_01495"
     CDS             complement(13984..14979)
                     /gene="hypE"
                     /locus_tag="PLV90_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HOF95818.1"
                     /translation="MKVNLMHGAGGEVMGELLQIITNLKHTNAGGIGLESLDDGAVIP
                     MCGQNVVFTTDSHVVSPIFFPGGDIGRIAVCGTINDLAMMGGRPIALSCGMVIPEGFE
                     VADLERIVASMDIALGECGANLVTGDTKVLERGALDTIIINTAGIGVADRVVRDNGLL
                     PGDAIVVSGTIGDHGIAIMAHREGFDFGGQIRSDVAPLWMLVERALAAGDIHAMKDPT
                     RGGFANAINEMAKKSGVGVVIEEEALPFKKNVKSAAGMLGIDPLEVANEGKVVMGVAP
                     GDAEAVLAALRSHPYGRDAAIVGKVVEGSHVVMRTAIGGERFIEPPIGDPVPRVC"
     gene            complement(14976..15317)
                     /locus_tag="PLV90_01500"
     CDS             complement(14976..15317)
                     /locus_tag="PLV90_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOF95819.1"
                     /translation="MHEYSIAYDIYTTARRAAIENNAKEITCISVDVGRMAMVNPEQV
                     EFLFNVIIEEDPLFSGARLVCRDIEARTRCSCGYEGSERFVCPRCGKLPEVVAGMEIV
                     VTNIEIEVDEA"
     gene            complement(15317..15544)
                     /gene="hypC"
                     /locus_tag="PLV90_01505"
     CDS             complement(15317..15544)
                     /gene="hypC"
                     /locus_tag="PLV90_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOF95820.1"
                     /translation="MCIAMPAEVLEIKEGNIGVVDFGDLQQEVRLDLVDVSVGEFVLV
                     HVGFAIQRLSREEGLETREIFRQVHAAMMEE"
     gene            complement(15727..16359)
                     /locus_tag="PLV90_01510"
     CDS             complement(15727..16359)
                     /locus_tag="PLV90_01510"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOF95821.1"
                     /translation="MCSVTDHAAAWDEDYRRRGNLWGGAVAPLPDIPAGAAVLELGCG
                     NGKTLSALARHSSGAVAIDVSPRAVALARQRPDTASVHLAVADARHLPFCGETFDAVF
                     IVHVAGHLTGPGRRALASEVRRVLLPGGTTFFRGFSIEDMRAGKGEETEHRTFARGDG
                     ILTHYFTEDEVSDLFTPLLPVLVRTHRWRMRVRGRDLPRAEIEAVFQRSA"
     gene            16432..17598
                     /locus_tag="PLV90_01515"
     CDS             16432..17598
                     /locus_tag="PLV90_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95822.1"
                     /translation="MIVMDLQAPAVAILALALFTGILFSPSMAASEIADIEPPQPAAA
                     RNISTLLSGVEERLQGGRLLNFTPGQVADEPVENETPPPPVEEILEPVYDDDELDLLV
                     EENGISLMLLSLQNTCALFAWDEVPAKESGAALRALAAGLRRDVAAMDISEDREALKD
                     AFTRALESYEAAGTLLQGNAPLNRTTVDAAVEANLEGSDCLREASKYLERPLLKAPED
                     IAAISLSLPRPRTASASDEELMLLQRYVYEDRNRANDISLMFESVSKTRIYYARDEGG
                     EEVFAEPGRVFLLVKVKVTNLGHKGESRTYTVRTPDIRDFTLEYRKTTYTPIKFASGT
                     SLGVPYATATLGRYEVKAGYIVYDIPEALPLDECFVRVNLGQATSPVWAIGKMM"
     gene            17647..18687
                     /locus_tag="PLV90_01520"
     CDS             17647..18687
                     /locus_tag="PLV90_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95823.1"
                     /translation="MAPGGSHRNILLAVGLIGVILSIALLVPEACATAIPSLPEENAT
                     SGNASSFEAGPEDETLLPEPASDDERLESLIAGAGIPLLDVSVGQICAAYAGDDAALR
                     SRAADMASLVDGYRADAAALDISPDMAPAYEHFVAALDEFAAAAALDRSEDMPGRLAL
                     GTEHLSSALRACNPPASADAPPLLMSAGEEEPAPRFPDALGMGERFCYDDSRKENTIS
                     LIASSLTWSYAFQTTGTSPVRYEAAPGSYFLLVAVKATHLGHKGEGVNTRIQTPAESA
                     FTLLYGDATYRPLAPPGSTNRGGSYSRVTLGRGESLSGYLFFEVPEELDLSRAYLRAN
                     LGRESPVWLLDG"
     gene            complement(18769..19233)
                     /gene="msrB"
                     /locus_tag="PLV90_01525"
     CDS             complement(18769..19233)
                     /gene="msrB"
                     /locus_tag="PLV90_01525"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOF95824.1"
                     /translation="MSEKSAGTVKVCNAVTGKVEEVEKVVKSDEEWQRQLTPEEFHVA
                     RQGGTEPAFTGRYVDCKEDGLYVCVCCANHLFPSAAKFGSGTGWPSFFRPVSDLNIRT
                     AIDTRFFMTRTEVLCRRCDAHLGHVFDDGPPPTYERYCMNSASLRFVRKKDL"
     gene            19432..19824
                     /locus_tag="PLV90_01530"
     CDS             19432..19824
                     /locus_tag="PLV90_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HOF95825.1"
                     /translation="MAGCTGTPGVPGTGVPGPEETVAVPATHSPPGPQPSFSTTVTAP
                     EKRFRADMSCYWIVTGTVTNTGDAPGRNVVIRFMLIDDGSAMIRSTETILIPRFPAGE
                     TKIFTANPLPGDCDRQYRADIVVMHDIP"
     gene            19839..20507
                     /locus_tag="PLV90_01535"
     CDS             19839..20507
                     /locus_tag="PLV90_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOF95826.1"
                     /translation="MKILGINGSPRGSKSQTLRLVQAVLDGARSAGADVEVVDVCKLH
                     IEYCNGCTVCYERGECVKDDDFAALYEKMCESDGIVLGSPNYIDSVTAQIKTMLDRMA
                     DAIHCQMFIGKYGCAVSTAGGSGADDVVGYLNGILQTLGANTVGGVGVVFAGDPEAIV
                     PAEGRAYELGKRLVRAIEKKETYPEQEERHAAMLERMRTLILANKDRWHHEYDHWKAA
                     GRLP"
     gene            complement(20504..23953)
                     /locus_tag="PLV90_01540"
     CDS             complement(20504..23953)
                     /locus_tag="PLV90_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOF95827.1"
                     /translation="MAFFDRFRANVERLQQERDYAGLARALSAEDPCTRADAARALAA
                     LGVPAIPALLDALESARPASRNRMTESLVSTGIPSIPLLLAVILRASPALQASISRAV
                     AGAGDGMFEALLPALHHEQPAIRRAAVIALRDTGKKAVPHFAKMLHGKDPSVGKEAAN
                     ALAALHWAPDDPGEKAWFCYLREDWKELAKLQGAAVPILLKALCSNRARERCDAARTL
                     GKIRDGRTIPALIGAMKDPDIDLRIHAAEALGEMGDDRAKPALANALNDPHHRVRMEA
                     AWALNRLGWVPPNDLARAEHLVAGEQWNELVRMGRPAIPALIRALEVEYSGVRTGASE
                     ALRQLGQPALTALKTEIVSKNPARQQRARMALEYIRRRQEEASRTGPAPADSSRYEQE
                     LKEGLAIQRRFVKQFGRERPLPSVPVKKPQPKEEEAPAPPVPESTPVQASEEPKKPVV
                     LEDLIMESQQAEEAWARVKAQFKQEVSVPAGAIPLEQLIPLEFEEAIAGSDSAGETNE
                     EPARKEPGPRELDIPDIPQKAPKPDIDEPVPEKTPLEQYLEALKSSDESIRATAIAAL
                     QAMGKEAIVYLIAALKDPHYSVRIAAVEALGELKDDAAVDALILVCADIREDVRIAVA
                     RALGSIGDRRAIRPLIRLFGDRYHGVRVAAADAVAVFGRDALGPLEDALDDPAPPVRV
                     TAAKAIGLIGAGESVPALIEHLGDAAPEVRWSVARALAGFGPLAVEPLFLVLRKGKKE
                     MRLAAIDALWEVPGERADEALLYASEDEDGDVREKAAAALRKRQVINVWRMALGSQIR
                     MGESTQKKRSARFEDTAAFEASGQQEIDTLIVALKDKAWNAQLGAATRLIMMGRPAVD
                     GLIRALRNENPDVQTAAASLLGEMRETAVAPLMDALNDTERFVRLVAARNLGKIGNKR
                     AIEALIGSLHREPDSEVRATVAEALGYMGSRQAIEPLVLALRDRDEAVKIAAARSLGY
                     MGDTRALEPLILALADPDDRVRHIALDILKDPDETVRGCLIGALRSGDEVLRAGAAEA
                     LSAGGWVPGTGEEKALHLMARDQWADVERIGADALPAVAGALANPSVEVRINAVGTIA
                     RIGGDAAIVPLIRALRDDALAVRAKAGQALVDMGDAALPALAAEERRPEVRQIVDEIR
                     RGT"
     gene            24103..25161
                     /locus_tag="PLV90_01545"
     CDS             24103..25161
                     /locus_tag="PLV90_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95828.1"
                     /translation="MLEVRLDITRLPGIVRELAPDTGDLVSRIYSWYVEPGNLVFPDA
                     MQAWVREKYGDIERQQIVRVTNDRTGESTLFNSVRARRPVLTPRAGETRDLEALAEGG
                     PFADPLNETPADTFGRIDGDHWITASNIARYDYLHAVIIAKEKNPYVVTEPQVADMVS
                     VAVRWCQEAHRADSRAVYPFIVWNYLWRAGASIVHNHAQVLLTHRPYAAPARLEQARE
                     EYRCRYGCEYYDDLFSAHVAIGLGLAYRGARVMAYLTPRKEHEVAIITESPHDIAPAL
                     AKVLACYRNIGVQSYNAAVYMPPLGEEGHYVTWVVDRGDLHSRTSDIGGMELYAGTPV
                     VTSDPFRLMEHLAAVMLP"
     gene            complement(25163..26311)
                     /locus_tag="PLV90_01550"
     CDS             complement(25163..26311)
                     /locus_tag="PLV90_01550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOF95829.1"
                     /translation="MKHLKIAFFCWESLYAERVGGLANAATNLAETLAQRHEVHFFTR
                     GEGRDAEINNVHYHYCRPAGEDIVRYCSDMSQKMLDRFGEFDRPAFDLLHFHDWHVVD
                     ALVRLRERETVFTYHSTEFGRSGNRYSDEWLFPAISGAERRGGEIAKRVTAVSSTLRR
                     EAMNLYDIPDWKIDVVQNGIIPGHYRADVDPEAVKQRYGFDPGAPLILFVGRLMWQKG
                     PDMLVDAAPRLLQEHADSQFAFVGDGQMRRGLEAQARSLPVRFLGRLDDADYVSLLNA
                     SDIVAIPSRNEPFGLVLLEAWSAGRCVVASDVGGLAENIEHGTDGVKVRPRPDSIARG
                     LSLASDSPALSLAMGRKGFDKVMEEFPWSRVRERMENSYRKARETLLC"
     gene            complement(26504..26995)
                     /locus_tag="PLV90_01555"
     CDS             complement(26504..26995)
                     /locus_tag="PLV90_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HOF95830.1"
                     /translation="MSQQSTEQKRFLADRMLGTLTRYLRFMGYDTMSANSIAPGMARE
                     DTLLLEIATRDGRLLLTRDRELARRGGAQAVYIVSEDIMEQIRQIAGLGLIEPRVRMN
                     RCSICNTHLRPATAREVREAREAREEDREREFSWCPVCRKLYWMGSHGEHIEERLRES
                     FSP"
     gene            complement(26976..>27763)
                     /gene="amrS"
                     /locus_tag="PLV90_01560"
     CDS             complement(26976..>27763)
                     /gene="amrS"
                     /locus_tag="PLV90_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOF95831.1"
                     /translation="LGTIGCNFHCEHCQNWHISQQTLEMEALRDLSPEQGVERAIRSG
                     SASIAWTYNEPTIWHEYPLAMGTLAREKGLGAVYVTNGYITEEGLREASSMLNAFRVD
                     IKSFSDPFYRKVCGGRLQPVLDATALAKELGMHVETVTLVIPGQNDSMDEMEMLIRWV
                     LDNLGPDTPMHFTRFHPDYRMLDARPTAVRTLEKIYERAKELGLNYPYLGNVGGHPYE
                     NTYCPACGSLAIERSGYAVRIRGIDDHTCTECGGRIEYVSTVD"
BASE COUNT         4628 a         9057 c         8697 g         5381 t
ORIGIN      
        1 agttcctgtt tgccgggagc atcggcgccg atcgcgttga tatgcgtccc ctccccgatc
       61 cagtccgcca taatgagggg ttttcttgac ggcgtcgtcg tgttcaggac gtcgcagtcg
      121 catgcgcgct cgatcgagac gcttaccgcg ttgtaatccg cgtaacgtgc tgcaaatgcc
      181 tcgctactct tctccgtcct gctccagacc cggatctcat cgatcgtgag cgttgccgca
      241 atcgcctcga cgagcgcctc cgcctgccgc ccggcaccca cgaccccgag ggtgacggtc
      301 cgccggggtg agagatactt cgccgctatg gctcctgccg ccccggtgcg catcgccgtg
      361 agctcggtgg cgttgacgat cgcctgcggt acgcctgttg tcacgtcgat gatctccgtg
      421 agcgccatga cggtaggcag gcctcgtgca cggttgttcg ggtggacgtt gacgatcttc
      481 accccggcta tcccgagcgc cggcatgtat gcaggcatcg tccggaaatc cccgttctcg
      541 acgagcccca cgtagacctt cgacggcatc cggactcctc ctctgccgtg ctctgcaaac
      601 gccgcctcga tcgcctggtt tacccgggtg tacggcgggt atccggagtg aaccgggtag
      661 tatctcatag tgcggtcacc tcacggcggg gcgattgggc cgggagcctt tcccgggctc
      721 ccgccggcca tacccctggt acagtccgga gttgcatagc tttctctccg atctgccggg
      781 atatcaacct gcgggtacag acccctctct cctcactcag ccccgtatcg agagccggat
      841 caggccgagg ttggcatcgg gttttgcaag tatgcagaga gagaggtcac cgtagggtgc
      901 gatgatgagt ttccctgccg gggtctcgag gatcacctgg gcaaggtccc ccatatccat
      961 atcggcggtg atgcggacgc cggcgcggag caggtcttcg gcgaccgcgg ccacctggtc
     1021 gaagtccgcg gaaccgagcg actggagggc gaacccctcg tggaagaccg agacggcgat
     1081 caccccgggc tgccgtgcga tccggtcgag gcgagccgtc tcccccgaat tagcccggga
     1141 ggcgggtgtt ttgccgtccc cgctgatcag gcaccccgct tcccgggcta cctcccgggc
     1201 cgcccccgtc tcgtcggcgg tgtaggcgat gagttcgtag tcaaacgacg gccggtcgag
     1261 caggtaccgg taggcctcgt ctcccctgag gggcaggcct cccccggtac cctcgaacgc
     1321 tgccgcgtag gggttcccgc cctgggcgag gatgaacccc gtccccccgg gccccttgat
     1381 acgcaccgct cccgtgaagc gggggctgat ggaggcaagg gccgagaggg gcccctgcag
     1441 ccgtccgagc gagatgccgt cggggagcat cgtctcacag ggcggcggtt atcctgtccc
     1501 tgttcttctt cagctcgtac cggatccgcc cgacgttgac gttcgcgttc gccacgatgg
     1561 cgatcagctc gtcctcggag agcggggaga ggaggatggg gccgttctcg agctcgacga
     1621 gcatctggtg catctcgccc ttcccgagtt cgttgctcat cgcctccgac gtacccatgc
     1681 ccgtcgatgc catggcgccc agggcctcga catctatgtc gccggcaaca acgctctcga
     1741 tcacaaagcc gtcccgtccc gccacgaccg cagcggtaac cccgtcaagt ctcaggaact
     1801 ctccgagaat ctgtcgtaac atctcacacc acctcaggtt cttctcatac attcgattct
     1861 aaggggcatc cgcccgatgg cgtgggtcgc acacgatatg caggggtcgt atgccctgat
     1921 gatcatctcg atgcggtttg ccgccccttc ggtcaacgtc ccgttcttca ccacccggcg
     1981 cgccatatcc tcgacacccc ggtcgatcgc gtagttgttc tggcaggtcg cgacgatcag
     2041 gttgcacttc tcgatgatgc cggcatcgtt cacggtataa tcgtggatca gggtcccccg
     2101 gggagcctcg atgatcccga cgccccgccg gttcacgacc ttccctgccg gtgtccttat
     2161 gccggaaccg gtgatgccgg gatccgcgag caggtctttt gcccgctcgc aggaggcgag
     2221 gaactcgatg tagcgggcga ggtgataggc aaggggtgcc tgggtgacgg tgccgaaccg
     2281 ctcgcggtac tccgcgaggg cggcgtcggc atgctccgtc cccatcctct ccacgatgtt
     2341 gagccgggcg agcggcccga cccggtagta gcccccgcct ttgagccggg cgaacttcag
     2401 gtaggaccag tcttccgagt actcctcgac gaagttcgtg taatcctggc ccgagaacga
     2461 gcctatccgc ccaccgtcgg ggtcaagcat cgccacaggc ccgtcgtatg tcgagtagag
     2521 cccgtggttg gtcataccca tgaacccggt ccggacggcg ccgatctccg tgtcgacccc
     2581 gtcgaggagg ttcttcgcga tctcccagcc ctcccgggcg atgccgagcg cctcgtccgc
     2641 catggcggcg agctcatccc tcttcccctc cgtgagcggc gccgacatgc cgccggggag
     2701 ggcgtttgcc gggtggatgg gtttgccgcc gaccgcctcc gtcaggcgct ggccaaactt
     2761 cctgaccgtt atggccttcc tggcgatgtc cggcgagtgg cgtgcaagcc cgacgacgtt
     2821 cctctcctcc ggcggcgcgt cgggggagag gatgaagtcg ggtgccgcga gcatgaagaa
     2881 gtggagggcg tgggaatgga cgaactgccc gagccccagg agctcgcgga ggttctttcc
     2941 ggtcgcgggg ggtgtgacgc cgaatatccg gtcggtcgcc ttcgccgccg cgaggtggtg
     3001 cgccgagggg catatgccgc atatccgggg agtgatccgg ggtgcctcct cgatagccgc
     3061 cccgacgagg aacttctcga acccccggag ttcgacgacc tggaagtggg ccgagtcgac
     3121 ctctccccgg tcgttgagat agatcctgac gccggcgtgg ccttcgatcc gggtcaccgg
     3181 gctgatcgtt atctccttca tcggctcctc tccttcacga ggtcgcggat cttcgacgtc
     3241 tccttgtctt ccatgatcag gctcccgatg gtgaaggcgt acatcgtatg ggcgacgtcg
     3301 tagagctcct tctccacctc ttttgccggt ttatcggtga gatccgatat cctcctgacc
     3361 atcatgccgt agatgtcgcg gcagggttcg cggaggatat cgagcgacgg gccgttgcac
     3421 ccgtggcagg gtatgttgtt cttcgggcac gttgcaccgc acctgccgaa ggtgacgctc
     3481 ccgaggcaga ggtagccctg cccgagcaga cagtgttcgc ggtcgggaac gccttcgtgc
     3541 cgccgccgga gcgtccagtc cccgaccggc cccatggtcc ggtcgcactc cgagcagacg
     3601 gacttgcgcg agagttcaag ggtatccccc gcaaccatcg ccgggatgat cttcttcaag
     3661 aacgcctctt ttggaggaca gcccgtgacg tagtaatcga tcttcacgag gtcgcccacc
     3721 gcgaacgccc ggtagaggaa cgggggcaca tccgtcggta taaccccgtc gggttttgcc
     3781 gtctccaccc cgcggtagac gcacgagaag aggtcctggt tcgagctcag catcgagagg
     3841 cccgatatcc ccccgtagca ggcgcaggtc ccgagagcga cgagcgtctt tgcccgtttc
     3901 cggatctttt cgaggcgttc ccggttctct tcgtttctga cggctcccgt cacgaacgct
     3961 atgtcgatac cctcgggggg ctccttcacg tccatgatca ccggggagta gacgatatcc
     4021 gccttcgcga cgacgtcaag gagcatctcg tggaggtcga gcaccgcgat cgtgcacccc
     4081 gaacatcctg ccagttcttc aatggcgatc ttcatcgtct ccacctcatt tcaggaccag
     4141 tttcttcagg caggcgttcg ggccggtgtg gacgatctcc agcctggctt tccggccggt
     4201 gatctcctcg accgcgccca cgacgtagcc aaagagggtc tggcagagcg ggccgccctg
     4261 gtcgaggccg ttgcgccgga gcgtctggcg gacgatgcag tcgtggaaga cgaggtagat
     4321 gaaggtctcg ccgttctctt cgatgatgaa cgtctcctta tcctcgggtt tccagacctc
     4381 gaaggagtac tggcccctga gcaggtcgga gagctcgtgg agagcgccct ccacgtcctc
     4441 gcgcttctgg aagtacttgg cgacctcgtg gccgaacttc tttcctgcac ggtaggtgac
     4501 cgcgtttgcc cctctgccgg ctatctcttc aagcgacctg ataacgaggc cgttgagttt
     4561 catcacgccg tgaagcgtct gctccagatc ttttggcctg ggcgaacagt cgatagggat
     4621 atcctccggc cggtagacga gatcggagtg gaaggcctcg tggagttcgt cgatgtagcc
     4681 cgcacccatc agatcacctt ccggagcatc tcacagacct tgatatcgat gtaatgccgc
     4741 cgggcagggt agatgccgcg gtgctcgagc gcctgggccg ggcagatctc gtgacaggac
     4801 aggcacccca tgcagttatt cggcctgacc ggaacactca cgctgttctg gagctcgtat
     4861 acgtgcatcg ggcaatcttt gacgcagaga ccgcacccga cacacgcatc cttatccaca
     4921 aatatatcca ttatgagcct ctgcatctgg ttaccacctc ctgctattaa aacgtagtta
     4981 tatatagttt atctgctaca aatatttcct ttaagaggcc gggttatgga ttttgtgcat
     5041 tctcccggca tcagccgggc atccggcaac tccaccggca ctcacgagaa gaggatactg
     5101 tcttgttgtt ggcagtggag aaggggaggt gcaggtaccc ggacttcgcg taagctcgga
     5161 ggtatgagca gtgaagcgat cgcacgcaaa gccttgatgg ggagtttctg gtgcccattc
     5221 accggacttc gcgaacttcc gcggcaccgc gcgagctatc cggtgcagag agggatgcgg
     5281 tctcgtgcgt gaaccgcccg gcctggatgc tagagcggcc tcacgcggtg aaccgcgaag
     5341 gtccgcctgc tgccccccgt gcctggagac ggagaaggca gcagggatgc atcacgcttt
     5401 tgcccggaag agggcgcgtg caagccggtc gatgaaccct tcgcgctttg ccggcacgct
     5461 ctcatcggtg tactcgatgc cggctacatc ggcggatatc cgctttaagg cccgggacgc
     5521 ttcggagtcc gggtacttca ccacgatggg cgatctcccc gccgacgcac gccggacgtt
     5581 ggcgtcttcg ggtatgatac caagcacccg gacgccaagg agcctctcca tctgtgcgcg
     5641 gttgaactcg tccccgttgc cggcaaccct gttgatgatc gccccctcga catgcccgcc
     5701 gaccgtctcg gtcaggatct tcgtcttcaa agagtcgacg atcgacgata tctcaggggt
     5761 cacgacgagg atcaccccgt cggcgatcgt cagcggtatg accccgtccc ggcttatccc
     5821 tgccggagcg tccaggatca ggacatcaaa cccgctcacg agatcggcca tgacgtcttt
     5881 caaccgttca ggtttcgact gctggaaacc ctgcagagat agcccgcacg gcaccacctt
     5941 cacgccgtac ggcccctcgt agatggcgtc gcgaatgcgg gctttgcctg ccagcacctc
     6001 gtgcagggtc accggcagat cctcaagccc gagaatgagg ccgaggttcg ccatcccgac
     6061 gtcggcatcg aggatgcatg tcttcttccc ataccttgcc agcatcggcc ccaggtttgc
     6121 cgtaaccgtc gttttgccgg taccgccttt gccggatgca atcgtgtata cttttaccat
     6181 tgaatcactc gttatcgtat atcgaacccg ccgatcttac acccagcgtt gcagggcatc
     6241 gcgggtatcc tgttcgagcg cgttcatcag ttcggcagag agatgctctt ttgggcgggc
     6301 gatgccgacg agcgtctccc cgcggtaggg aataccaagg agctccgctc cggtcccgtc
     6361 ctgccgctct ccccgggcgt aaaggtgact gtaccgggct gcctcgccct cgctgcccgt
     6421 cttcgtcgaa ccgaccagga ggccgtccgc ggttgccagc gtgaaggatg caaggctgta
     6481 cttcctgctg atcgcgccca ggttctcctc caggctcttt gcacctcgag gatcgatatc
     6541 gccgggcgac tcccggttgc ctgccggtct ctcctgagac tcgatggcgc ctcccgggat
     6601 ctctttggcg agtgcctgag cgatccttga gaggtagtag agaacgccgg cgaaaccggc
     6661 tatcaccgtc accgtcatga tgacgaggaa gatctggatg atccctgttg aatccattct
     6721 gttgtcagcc cctgtctgtt cagatatctc cgtcctggat gaggtggtcg agatgaatgt
     6781 tcctgagcat ctctttgcag tttatccgga agttgctgat gagttgttcg acatccatct
     6841 tttccatgtt ctggatgagc atatcgatct cgtcgttccc ggatggagtc gctgcaacca
     6901 tctcctgcac aggtctcgtg cccggcatgg ggatcctgtg cccttcccct gtcggttccg
     6961 cgcggtgctc cggtgagcat gccgcagtgg gttctgccac tccagcgagt ggtcttcccc
     7021 cgcccggtgc gggttttgtc ggtgatgttc ttttctccct gccggcgtcg ccggcatcaa
     7081 tcgcacacgg ttggttgaac tccctggcga gctgtacctg ctcgggtgtc aggaggttca
     7141 gttctgccgc tgcttcgctc tcatcgttcg atacggcgtc cagtgcgtgc tgtcctctta
     7201 tgcccccata ttcggcaagc accaccagac cttcattcag caccaggacg gcactcttct
     7261 cgccagggac gattgtacag atcccggtga atcgcgcgga agccatctcc tctatcagga
     7321 cacgggatgt cgtggacttg ataaggcgat ggaaccgacc tcgtggcagc tgcatacgga
     7381 catcctcaat actgttgtat gagataaacc ttgtcattta tactttttga aacgggcatg
     7441 gtggtctcgc gcccctgcac atcatagtcg cactcgtgaa ctgccctggc ccggagaggg
     7501 gctctcagat ccgcccccgg gtttgctgcc ggcaagggtg ccgcaaggct catccatcca
     7561 gtcccatagg agcctgacga cctccttttc ggcagcccgg accccccctt cgaccgccgg
     7621 gtctacgccc cggtgaaacg cccgtatgtc gcccacctcg atgccgatgg tcacgatctc
     7681 cgcgacgcac ccgagttctc gggccactgc caccgcgtcg ccgatgccga tatcgtggag
     7741 cgagcatgcg ggaaggtcca gagagggcgg tgttgcaaag acgacgacgt cgccgatacg
     7801 gttgccgatg cccgtcatcg catcgacgat cacgaccttc tcgtacccct ccatcagggg
     7861 tatgaggccg aaccccccgg tgccgccgtc gatcgcgtcg acgcccgggt acctcccttc
     7921 aaagagctgc atcaccagga tccccgctcc gtcattcccc atgaaggggt ttccgcaccc
     7981 gatgatgccg atccggttct ctcgcatctt cgccttcccc actgattata tccggcgagg
     8041 tacaaaaggg tactgagacc cggggagcaa atcgtcagtc ccgggagcgg aatgtgaggc
     8101 gtatgatcca tatcgtacct aaaccgaccg atacgccgga cgacaactcg accggcgcgg
     8161 tgatacggga gctggagagg gccggtgccc gatgcagggt catcgatctc ggcacgatcg
     8221 atcctctcga ctccgggctt gagaacgaac tcatatgggt ctgcgggatc cggcaggacg
     8281 gccatcagtt cgagaccctg agcgtgcttg cgctccataa ccaggtggtc aacacgcccg
     8341 agagcatcgt cacctgcgca tccaaagtga tgacgaccgc ccttctcctc catgacggca
     8401 tccggacgcc ggagacggca tacatgcgct cggaggcgca ggccggggcg ttcctcaagc
     8461 ggcacggaaa ggtcgtctat aaaccgctct acgggtttga cggcaacggc atccgcctgg
     8521 tggagagacc cgacgacttc gggccggggc cctggtatct gcaggagtac gtgccaaacg
     8581 accgggactt ccgtgtattc gtcatcggtg gagaggccgt cggcgcgata gcccgtgtat
     8641 ccgagggcct gatgcacaac atccaccagg gcggcatcgg ctcgccggtc ccgatcgacg
     8701 aggagatgcg ggccatagcc gaggcatcgg cgcgggcggt cgatatcgac tactgcggcg
     8761 tcgatctcct ccgcgataag gagggttata ccgtcctcga ggtgaacggg acgccgaact
     8821 ggcactgcat ggcggcgcct atcccgcggc tgctggcggc gtacctcatc gagcgcaggc
     8881 gcgccatgcg gggttgagga gaggagcgcc ggggcaaggc cccggccggg caccctgccc
     8941 gcgggcgatg cggggcagga aagtcgttac tggagatact cgatctcttt taaggccttc
     9001 tcggcgagtt ccttcatccg ggcagatatc ctcccggatg atgagaactc cacgtccttc
     9061 atggcgttgg cgagttccgt tccgagcacg aatattgcgt atttatgttc catcttgctc
     9121 ttatgcacct gagaaggatc tatctttaat gcgtaatact gtgaaaactt caactccgga
     9181 ttcatcaatt caaaatagtc tttaatctcc gtaagtatcc catgtaaggc tattaactct
     9241 tccttgtgca tattctctcc cggagataaa aatagtagat ataaacatat ataaatttgc
     9301 accccggata atcttcaccc tatctttata agcctgatgg agagagggcg cttcacgatg
     9361 cctttgaagt cgcgggcggc cacgaactcg aggtctcttc ccccgatctg gtcgaggagc
     9421 ttctggtcga cgatgcggtc gacgacaagc ccttcgacgt cgccggcgag cgtctggagc
     9481 gctccctcga cgtcggcggc gttcgactcc gccagaacgg tgtagtcggg ggagaggaac
     9541 cgtgcgatct ttctgtcccg gacgtcggcc atgtgctcgc cgagagtcga cggaggtctc
     9601 cggctcccct cttttgccgc cggccgctcg ccgctgctcc cctcgcccgc accggcagga
     9661 ggtcgcgctt cggcagggcc ggcagggggg ggcaaccgtt ccgccgccgc tccttcgtcg
     9721 acgatgtgat cggtgaccac ctccacgggc actttgttcc ggagcgcctt gacgatctcc
     9781 ttgcggctca tctcctcgac gctcttcccc cgcgggctgt aggccacgaa gtcgatctcg
     9841 gcgacctgga ggagttcgcg gaggatcagt tccccgcccc ggtctccgtc aagcagggtc
     9901 gtcgcggtct tctgcgaaca gaggtcgatg atgacgcgcg ggacgttggt cccttcgacc
     9961 gcaaccgcgt tcttgatgcc gtaacgcagg aggttgatga cgtcggcccg cccttcgacg
    10021 aggatgatgg catcggaggc catgacgttc gggcccgcgt gcaccctctc ctcgccgagg
    10081 tactcgagtt tctctatcct gatgacctcc ctgacctcgt cgagcaggtc gtcgctgctt
    10141 atggcgcctt catcgaagtc ttcgaggagg agctcttttg cgcgctcgac gatcttgcgc
    10201 cgcttggtca cccggatgtc ctcgatgcgg tcgaccttca cgcgggccgt gcatggcccg
    10261 acccggtcga tcgtctcaag cgacgaggca aggagagccg tctctgcccg gtcaagcgac
    10321 gacgatatga ggatctcgcc tttcgtctcg cctctcttcg tggttatctg aacgtcgatc
    10381 ctgccgacgc ggccggttct ctggagatcg cgcaggtcga ggtcttcacc gaggagaccc
    10441 tcggtctgac cgaagatggc gcccaccacg tcgggcttgt cgaccacccc ctcggcctgc
    10501 agagtaagat gaatgaggta ctttgtggtt tctggtgaat acatggagtc atgcctcctt
    10561 cctggtaagt atgatcgccc tcgtgccgcg cgggtcacgg tatgcgcggg cagaggatca
    10621 attatatatc gaaggtgcaa ttacttaaat cagatggggg attgaacata aggtttttta
    10681 aaagagaaat ccaaatttga gtcttatcca ggcatctttt gaatttatgg cgggttatat
    10741 atcctccgct ataatagcaa ctcaatcctc gtgatatacc tccggatcat gcaccggcgg
    10801 cgttgaggca ggcgaggaca ccgtctctcg atcgtccggc gaccgcgacg gtctttgatg
    10861 aggacgtgtg cccggatagg atgacgacgt cggcacgcgg cacgccgagc gtccctgcga
    10921 cgaggtcgac gacggcgcgg ttcgccttcc cgccgacggc aggggccgcg acctggcagc
    10981 ggatgctctt gcgccattcg ttgtagcccg ccggaaagga cgaccgtttt gcgcctgcgg
    11041 tgacgtcgag cgtgatgacc acgccatgtg cggtttcgac gacggcatcg gcatacggtt
    11101 ccataaaaga gcctcgtgat atgtcgcccg gcactaacga tcgcggatca caggcggctt
    11161 gtgccggtcc tcacccgatc gtaccctata tgccgggcgt gcctgtcgcg cacccgggtt
    11221 gtcccatgga tcatatcggg cttctgccgg tgatcacccg gggacctata aggtgcgctg
    11281 tatcggctct atattattct atctataatg ataaatatcc ttgcccgggg taccacgccg
    11341 ggtgcgcctc caggagttcg ccgccggcgt aggtgcgcct gccccggacg acgacctcct
    11401 cggggaagat ggcatcgagc ccttcgaacg gcgaccaacc gcattttgcg tggagactcc
    11461 ctgcatcgat gcgggtgacc gcatcggggt agagggcgaa gtccgcccgg tcgccgggga
    11521 ggaacccggc gcggcgtatg ccgagtatgg cggcaggccg ccacgacgtc ttctcgatga
    11581 ccgaggcaag gggtatgtcg cccctccgca ccgccgccat gagaagcggc accatcgtct
    11641 cgacgccggg cataccggag ggggcaccct caaacggcag ctctttctcg cggagcgtgt
    11701 gcggggcgtg gtccgaggcg acgatgtcga tcctctccca gcaggaccag aggccgcgcc
    11761 gggtctcctc atccctgagc ggggggttca cccgggcccg ggcgtcgtcc tccgcgaaca
    11821 tatcgcgcga gaggaagagg tggtgcggcg tcgcctccac cgtgccccgt gcggcctcga
    11881 ccgaggcggc ggtgctcatg tggcagaagt ggagccgcat gccgccgggg gcgagagcac
    11941 ggaccgccct gacggcgcgc gcctccccgg cgccggagcg ggcacggtcg tgctccgcaa
    12001 gcgtcgccgg cgccgggcct gcgacctcct cggcgtggac tgtggcaagc ccgcccatgg
    12061 cgtggatgcg ggcaaggagt ctttcgaggg tctccgggtc aagcccttcg ccgtaacttg
    12121 agggggcggc gaatatctcg ccgaacgcca tcgcaccggc gttacgcatc ccctccaggt
    12181 ctgcatccgt tgtgacgccc gcgttgacgg caaacccgca gacggcgtgc tcctctgcct
    12241 cgcggatgcg tgcgcaaagg aggtcgggcg tggtgagggg cgggatggtg ttgggctggt
    12301 cgaccaccac cgtcaccccg ccggcgacgg cgctctccgt ccccgtccgc cagtcctcct
    12361 tctctgcctg cacccctccc cgcatgtgaa cgtgcatatc gaccgccgcg gggagacagg
    12421 tcaggccgct gcagtcgatc gtctcgtcgg cacgcaccgc tcccccgacg tgccgcacca
    12481 ccccgccggc gatgacgata tcggcagagc gccccgtcgg gagcaggacg tttttgagca
    12541 ccaggtcggc ggtcaccggc ggcacgcctc caggtcgtcc cgggtcgcgt agtgcacctc
    12601 taccatgcag gcggggtggc agcgcgtctt tgactcgcgc agccccggca cgcccatatc
    12661 gcactcgcgg ttgatgaagg ggtagcgcct gcggagtcct gccgccgtct cggtcatgat
    12721 caccttgtag atatcccggt aggtctcggg gagggctttc tcgaagtgga cgaccgccat
    12781 atccgcgttg acctcgtcga cgaccgatat cgccccgatg gtctcgccaa cccggatgat
    12841 ccagccccga agcccgatcg cgaagaagtt gttgatcgca aagaggatgg cctctttctc
    12901 gtaggcaagg atgggcacgg agtcgcagtc ccgccactcg caccagatga ccaggaactc
    12961 ccagacctcg tcgatgttct cgcgggtgat cgcctcgacc gagtaggcgt actccctcct
    13021 gaagtggttg acctggcgcc ggacggtggc atagcccttc ccggcaaagt cggcgaggtc
    13081 ttctgtcctg tagatgtagt caaagtagtc gcggtcggga taaaggatga ggttcgggta
    13141 gatttcgcgg atccaggctt cattcaccgg gtcgaggacc agcaggggag ctgcgccccc
    13201 ctccctcagg gcgaggtcga tgacgtcgtc gagcagggcc gggtttttcg ggccgatcgg
    13261 ggggcgaaac gaggttacgc cgtcgatggt gctcgagagg acgatcgcgc cgtccacgtc
    13321 ggcaaagcag tagttcgcat aatggttcca gcagaccatg ttggcaaacg tgttgtcgct
    13381 gtgcacctgc gggtactgcc ggtactgccg caggaagaga tcgcggtcgt ccaggctcac
    13441 gggtttgaac tcggagaact ccagcaacgc ctcacctctc gtccgggtac caccgcatgg
    13501 gcgtgtgccc ctccatcctc cggaaaccga gcggcgtata gaacgcttcg gtcccgggtt
    13561 cggcgacaag ggcgacccag gtgacacctg cagatttaca gtgatcgagc agcgttgata
    13621 agatcatcgt tccgatgccc cggccccggt acccggggag gacgaccagg tcctggatgt
    13681 aggcatcaga gactccgtcc gatatcaccc tgcccatgcc gaccgctttt ttcgccgccg
    13741 ggtcgatggc gacggcaaag gcaaaactcc cggcgatgag agcacggagt cccgcggggt
    13801 cccactcctc gttccaccac ccgcccgccc ggtagagatc ggctatcata tccccgtccc
    13861 agacgcttac caggcgcacg tcgatcgctc tctgctcgat catatcagca taccactcca
    13921 ttgcgaccgc ataacgatgt gcatggtgga tcgggcgggc ataaaaatag gtgtgaccgc
    13981 ccgctaacag acgcgcggga ccgggtcgcc gatcggtggt tctatgaacc gctctccgcc
    14041 gatagcggtc cgcatgacca cgtgggaacc ttcgacgacc ttgccgacga tcgccgcgtc
    14101 ccgcccgtag gggtgcgacc ggagcgccgc gaggacggcc tccgcgtcgc cgggcgcgac
    14161 ccccatgacg acctttcctt cgtttgccac ctcgagggga tcgatgccga gcatgccggc
    14221 ggcgcttttg acgttcttct tgaaggggag ggcttcctct tcgatgacga cgcccacgcc
    14281 gctctttttt gccatctcgt tgatggcgtt tgcaaacccg ccccgggtcg ggtctttcat
    14341 ggcgtggata tcgccggccg cgagcgcccg ctcgacgagc atccagaggg gggcgacgtc
    14401 ggagcggatc tggccgccga agtcgaatcc ctcccggtgg gccatgatgg cgatgccgtg
    14461 gtcgccgatg gtgccgctga cgacgatcgc gtcgcccggc agaaggccgt tgtcccgcac
    14521 cacccggtcg gcaacgccga tgccggccgt gttgatgatg atggtgtcga gcgcgccgcg
    14581 ttcgaggacc ttggtgtccc ccgtgacgag gtttgccccg cactcgccga gggcgatatc
    14641 catcgaggcg acgatccgct cgaggtccgc gacctcgaac ccttcgggta tgaccatgcc
    14701 gcaggagagc gcgatcggcc gcccccccat catggcgagg tcgttgatcg tcccgcagac
    14761 ggcgatgcgt cctatatcgc cgccggggaa gaatatcggc gacacgacgt ggctgtcggt
    14821 cgtgaaaacg acgttctggc cgcacatggg gatgaccgcc ccgtcgtcga gcgattcgag
    14881 ccctataccg ccggcgttgg tgtgcttgag gttggtgatg atctgcagga gctcgcccat
    14941 cacctcgccg ccggcgccgt gcatgaggtt gactttcatg cttcgtccac ctctatctct
    15001 atgttggtaa cgacgatctc catgcctgcg acgacctccg ggagttttcc gcatcggggg
    15061 cagacgaacc gctcgctccc ctcgtaaccg caggagcagc gcgtgcgcgc ctcgatatcc
    15121 cggcatacga gccgggcgcc cgagaagagg gggtcttcct cgatgatgac gttgaagaga
    15181 aactcgacct gctcggggtt caccatcgcc atcctgccga cgtcgaccga gatgcacgta
    15241 atctcttttg cgttgttctc gatcgccgcc cggcgggcgg tcgtgtagat gtcgtaggcg
    15301 atgctgtact cgtgcattac tcctccatca tcgcggcgtg gacctgcctg aagatctcgc
    15361 gggtctcaag cccctcttct ctgctgagac gctggatggc aaacccgacg tggacgagga
    15421 cgaactcccc gacgctcacg tccacgagat cgagcctgac ctcctgctgc aggtcgccga
    15481 agtcgacgac gccgatgttg ccctccttta tctccagcac ctcagcgggc attgcgatac
    15541 acatgaactc tcttcctccc tgttagtttg tccgggcttc aaaaaaggtt gaccttgctg
    15601 cacaaagggc ccggggatgg gccgttttag ccggcgtgcg cccccgggcc cgtatatact
    15661 gctacgtagt attcggtctg gaagtgtaaa aaagtatgtc cgaccgggga agtgcggcac
    15721 tccgcttcat gccgatctct gaaacaccgc ctcgatctcc gcacggggga ggtcgcggcc
    15781 cctgacccgc atccgccagc ggtgcgtccg gaccaggacg ggcaagagcg gtgtaaagag
    15841 gtcgctcacc tcgtcttcgg tgaagtagtg ggtgaggatg ccgtcccctc tcgcaaaagt
    15901 ccggtgttcc gtctcctccc ccttcccggc acgcatatcc tcgatggaga acccccggaa
    15961 gaaggtcgtg ccgccgggga ggagcacccg acggacctcg gatgccaggg ctctccggcc
    16021 cggacccgtc aggtgaccgg ctacgtggac gatgaagacc gcgtcgaacg tctccccgca
    16081 aaacgggagg tgccgggcat cggcaacggc cagatggacc gacgccgtat ccggccgctg
    16141 ccgggcgagg gcgacggcgc gaggggatac gtcgatcgcg acggctccgg atgaatgccg
    16201 ggcaagggcg gagagggtct tgccgttgcc gcagccaagt tccaggacgg cggcgccggc
    16261 gggtatatcg gggagcggcg ccaccgctcc gccccagagg tttccccgcc tccggtagtc
    16321 ctcgtcccat gcggccgcgt gatcggtcac gctgcacatg tcggcgtcgc ccggacataa
    16381 tggttcgttc cgcctgcccg gggaaggttt ttgtctcagg agaggtgaga tatgatcgtt
    16441 atggacctcc aggctcccgc tgtggcgata ctggccctcg ctctcttcac gggcatactg
    16501 ttcagcccgt ccatggcagc atcggagatt gccgatatcg agcccccgca gcctgccgcc
    16561 gcccggaaca tatcaaccct cctgtccggg gttgaggagc ggctccaggg cggccgtctc
    16621 ctgaacttca cccccggcca ggtggcggat gaaccggtgg agaacgagac ccctcccccg
    16681 ccggtcgagg agatcctcga gcccgtctac gatgacgacg aactggacct gctggtcgag
    16741 gaaaacggca tatcgctcat gcttctttcg ttgcagaaca cctgcgccct ctttgcctgg
    16801 gacgaggtgc cggcaaagga gagcggtgcg gcgctccggg cgcttgccgc cggccttcgc
    16861 agggatgtgg cggcgatgga tatctcggag gaccgcgagg ccctgaagga tgcgttcacc
    16921 cgtgcactcg agtcctacga ggccgccggc acactgctcc aggggaacgc ccccctcaac
    16981 aggacgacgg tggatgcggc agtcgaggcg aacctggaag ggtcggactg ccttcgcgag
    17041 gcgtcgaaat acctggagcg cccgttgctg aaggcgccgg aggatatcgc ggcgatcagc
    17101 ctctccctgc cccgcccgcg caccgcgtcc gcatcggacg aggaactgat gctcctgcag
    17161 cgctacgtct atgaagaccg gaaccgcgca aacgacatat ccctgatgtt tgagtcggtg
    17221 agcaagaccc gcatatacta cgcccgcgac gagggcggag aggaagtatt cgcggaaccc
    17281 ggaagggtct tcctgctcgt caaggtgaag gttacgaacc tgggccacaa aggggagagc
    17341 aggacgtata cggtgcggac ccccgacatc cgggatttca ccctcgaata ccgcaagacg
    17401 acctacacgc cgataaaatt cgcgtccggg acgtcgcttg gcgtgccgta tgctacggcg
    17461 acgctgggcc ggtacgaggt gaaggcaggg tacatcgtct acgatatacc ggaggcactc
    17521 cctctcgacg agtgcttcgt ccgggtgaac ctcggccagg cgacgtcgcc cgtctgggcg
    17581 atcgggaaga tgatgtgacc ccctccagcg caacgaatat cccttcggtg cgcaataggt
    17641 tagtctgtgg caccgggagg ttctcataga aatatactgc ttgcggtcgg cctgatcggg
    17701 gtgatcctct cgatcgccct cctcgttccg gaggcatgcg cgacggccat cccctctctg
    17761 ccggaggaga acgctacctc cgggaacgca tcctcgtttg aggccggacc ggaagacgag
    17821 acgcttctcc ccgagccggc aagcgacgac gagaggctgg agtcgttgat agccggggcc
    17881 ggcataccgc tcctcgacgt ctccgtaggg cagatctgcg ccgcgtatgc cggcgacgac
    17941 gcagccctcc ggagccgggc agccgatatg gcttctctcg tggacggcta ccgtgccgat
    18001 gctgccgccc ttgatatatc ccccgacatg gcgcctgcat acgagcactt cgtcgccgcg
    18061 ctcgacgagt tcgccgcggc cgccgctctc gaccggagcg aggatatgcc gggccgcctc
    18121 gccctcggga ccgagcacct ctccagtgcc ctcagggcct gcaacccccc cgcgagcgcc
    18181 gacgctcccc cgctgctcat gagcgccggc gaggaggagc ccgcaccccg tttccccgac
    18241 gctctcggga tgggcgagcg gttctgctat gacgattccc gcaaagagaa taccatatcg
    18301 ctgatcgcca gttcgctgac ctggtcgtac gcgttccaga ccaccggcac gagcccggta
    18361 cgctacgagg cggccccggg gagttacttc ctcctggtcg cggtgaaggc gacgcacctc
    18421 ggtcataaag gggaaggcgt gaacacccgc atccagacgc ctgcggagag cgcctttacc
    18481 ctcctctacg gcgatgcgac ctaccggccg ctcgcgccgc ccggctcgac caaccggggg
    18541 gggtcctact cccgggtcac gctcggccgg ggcgagtcgc tctcgggcta cctcttcttc
    18601 gaggtgccgg aggaacttga cctgtcccgt gcgtacctcc gggcgaacct cggcagggag
    18661 agcccggtct ggcttctcga cgggtgaagc cgtttcactg tccacaccct cgagatcgcg
    18721 cgaaggcctg tccgggcact tgcggccata ttggaaaggc gacggcccct acaggtcttt
    18781 tttccgcacg aaccggagcg acgccgagtt catgcagtag cgctcgtagg tcggcggcgg
    18841 cccgtcgtca aagacgtggc ccaggtgggc gtcgcaccgc ctgcagagca cctcggtccg
    18901 ggtcatgaag aaccgggtgt cgatggccgt ccgtatgttc aggtccgata ccggcctgaa
    18961 aaaactcggc cagcccgtcc ccgacccgaa cttcgctgcc gacgggaaga ggtggttagc
    19021 gcagcagacg cagacataca ggccgtcttc cttgcagtcc acgtatcttc ccgtgaaggc
    19081 cggctccgtg cccccctgcc gggcaacgtg gaactcttcg ggcgtgagtt gccgctgcca
    19141 ctcttcgtct gatttgacga ctttttcgac ctcctcgacc ttcccggtca cggcgttgca
    19201 gaccttcacg gtcccggcgg acttctcgct catcagaact cctcccggct ccttaccccc
    19261 cggaggtata aatcgtttcc ccgccggggc gggagcggct cgcggcgcct cccggttccg
    19321 catcagttat atagcctccg gtcgatgaac catcatgcag gagggtgaac tcccctgaag
    19381 aagagatctc tatatctcct gctcgtcacc tgcctcatcg gttctgccgt catggcaggc
    19441 tgcacgggga cgccgggggt tcccgggacc ggcgtcccgg ggcccgagga gacggtggcg
    19501 gttcccgcca cccattcgcc ccccggaccg cagccgtcgt tctccaccac cgtcaccgcg
    19561 ccggagaagc gtttccgcgc cgatatgtcc tgctactgga tcgtgacggg aacggtgacg
    19621 aacaccggcg atgcgccggg aagaaacgtc gtcatccggt tcatgctcat cgacgacggg
    19681 agcgccatga tccggtcgac cgagaccatc ctcatccccc ggttcccggc gggcgagacg
    19741 aagatcttca cggcaaaccc gctcccgggc gactgtgacc ggcagtatcg cgccgatatc
    19801 gtcgtcatgc acgatatacc gtaaatagag gtgaaataat gaagatactt gggattaacg
    19861 gaagcccccg cggctcaaag agccagacgc tccggctcgt gcaggcggtc ctcgacggcg
    19921 cacggagcgc cggggccgat gtcgaggtcg tggatgtctg caaactgcat atcgagtact
    19981 gcaacgggtg cacggtctgc tacgagcggg gcgaatgcgt caaagatgac gattttgccg
    20041 ccctgtacga gaagatgtgt gagagcgacg gcatcgtcct cggctcgccg aactacatcg
    20101 actccgtcac cgcccagatc aagaccatgc tcgaccggat ggccgacgcc atccattgcc
    20161 agatgttcat cgggaagtac gggtgcgctg tctccacggc aggcggctcg ggggccgacg
    20221 atgtcgtggg ctacttaaac ggcatccttc aaactctggg cgcaaacacc gtcggcggcg
    20281 tcggcgtggt ctttgcagga gaccccgagg cgatcgtgcc ggcggagggg agggcttacg
    20341 agctcggcaa gaggcttgtg cgggccatcg agaagaaaga gacctatccc gagcaggaag
    20401 aacgccacgc cgcgatgctc gagcggatgc gtaccctgat cctggcaaac aaagaccgct
    20461 ggcaccacga gtacgatcac tggaaggcag ccgggcggct gccctaagta ccgcggcgga
    20521 tctcgtcgac gatctgccgg acctccggcc gcctctcctc ggccgcgagg gcgggaaggg
    20581 ccgcgtcccc catatcgacc agggcctgtc cggcctttgc cctgaccgcg agggcgtcgt
    20641 ccctgagcgc ccggatgagg gggacgatgg ccgcatctcc tcctatccgt gcgatggtcc
    20701 cgacggcgtt tatccgcacc tcgacggatg ggtttgcgag cgctcctgcc accgccggca
    20761 gcgcatcggc gccgatccgt tccacatcgg cccactggtc ccgggccatg aggtggagcg
    20821 ctttctcttc gccggtcccc ggcacccaac cgcccgcgga gagcgcttcg gcagcccccg
    20881 ccctgagtac ctcgtccccc gagcggagcg caccgatcag gcagccccgc accgtctcat
    20941 cgggatcctt cagtatatcg agcgcgatgt gccggacccg gtcgtccgga tcggcaagcg
    21001 ccaggatcaa cggttcgagg gcgcgggtat cccccatgta gccgagcgac cgggcggcgg
    21061 cgatcttgac cgcctcatcc ctgtcccgca acgccaggac cagcggctct atcgcctgcc
    21121 ggctccccat gtagccgagc gcctctgcca cggtcgccct gacctcgctg tcgggttccc
    21181 ggtgcaggga gccgatcagg gcctcgatgg cccgcttgtt ccctatctta ccgaggtttc
    21241 tcgcggcgac gagccggacg aaccgttcgg tgtcgttcag ggcatccatg agcggtgcga
    21301 ccgcggtctc gcgcatctcc ccgaggagac tggcggcagc ggtctggaca tcggggttct
    21361 cgttcctgag cgcacggatg aggccgtcca cggcagggcg gcccatcatg atgaggcgcg
    21421 tggcggcgcc aagttgcgcg ttccaggcct tgtccttgag cgccacgatg agcgtatcga
    21481 tctcctgctg gccggacgct tcaaacgctg ctgtatcctc gaaccgggcc gatctcttct
    21541 tttgggtgct ctctcccatc cggatctggc tgccgagcgc catccgccag acgttgatca
    21601 cctgccgttt ccggagggcc gccgctgctt tctccctcac gtctccatcc tcatcctcgc
    21661 tcgcgtagag gagggcttcg tccgcccgct cgcccggcac ctcccagagc gcatcgatcg
    21721 ccgcaaggcg catctctttc tttcccttcc tcaacaccag gaagagcggc tccaccgcca
    21781 gggggccaaa ccctgcaagt gcccgggcaa cgctccaccg gacttccggc gccgcgtctc
    21841 ccaggtgctc gatgagggcc gggaccgact cgccggcgcc gatgagccct atcgcctttg
    21901 ccgccgtcac ccggacgggc ggggccggat cgtcgagggc atcctcaagc ggcccgagcg
    21961 catcgcgccc gaagaccgcg acggcgtcgg cggcggcaac ccgaacgccg tggtacctgt
    22021 ccccgaagag gcgtatgagc ggccgtatag cacggcggtc gccgatggac ccgagggcgc
    22081 gggcgacggc gatccgcacg tcttcgcgta tatcggcaca gaccaggatc agcgcatcga
    22141 cggcggcatc gtctttgagt tcgccgagcg cctctacggc ggcgatccgg acgctgtagt
    22201 gcggatcttt gagcgccgcg atgaggtaca cgatcgcctc cttccccatc gcctgcagcg
    22261 cggcaatcgc cgttgccctg atgctctcgt cgctgctctt gagggcttcg aggtattgct
    22321 ccaggggcgt cttttccggg accggctcat cgatatcggg cttcggcgcc ttttggggga
    22381 tatccggtat atcgagttcc cgcggtccgg gctctttacg ggcaggttcc tcgttcgtct
    22441 caccggcgct gtcgcttccg gcgatcgcct cctcaaactc gagcgggatg agttgctcaa
    22501 gcggtatcgc tcctgccggg accgatacct cctgcttgaa ctgggccttg acccgcgccc
    22561 acgcctcttc cgcctgctgg ctctccatga tgaggtcttc gaggactacg ggtttcttcg
    22621 gctcttcgct ggcctgcacc ggggtgctct ccgggaccgg gggggcgggg gcctcctcct
    22681 ctttcggctg cggttttttg accggcacgc tcgggagggg tctctcgcgc ccgaactgct
    22741 tgacgaaccg tctctggatg gcaagcccct ctttgagttc ctgctcatac cgcgatgagt
    22801 ccgccggcgc aggccctgtc cgtgacgcct cctcctggcg ccgccgtatg tactcgaggg
    22861 ccatccgcgc ccgctgttgc cgtgcagggt tctttgatac gatctccgtc ttcagggcgg
    22921 tgagagcggg ctgtcccagt tgccgcagcg cttcgcttgc gccggtgcgg actcccgagt
    22981 actccacctc cagcgcccgg atgagcgccg gtatggccgg cctccccatc cggacgagct
    23041 cgttccactg ctcgcctgca acgaggtgct ccgctcttgc cagatcgttt ggcggcaccc
    23101 atcccagccg gttgagcgcc catgccgcct ccatccgcac ccggtggtgg gggtcgttga
    23161 gcgcattggc aagcgcaggt ttcgcccgat cgtcccccat ctccccgagg gcctcggcgg
    23221 cgtgtatgcg gagatcgata tccgggtctt tcatcgcccc gatgagcgcc ggtatggtcc
    23281 ggccgtcgcg gatcttgccg agcgttcgcg ccgcgtcgca tcgttcccgg gccctgttgc
    23341 tgcagagagc cttgaggagg atcgggacgg ctgcgccctg gagttttgca agctccttcc
    23401 agtcttcccg gaggtagcag aaccacgcct tctcgccggg gtcgtcaggc gcccagtgta
    23461 gtgcggcaag cgcatttgcg gcctctttcc cgaccgaagg gtctttgccg tggagcatct
    23521 tcgcaaagtg cgggaccgct ttcttcccgg tatcccggag agctatcacc gcagcccgcc
    23581 ggattgccgg ctgctcgtgg tgcagggcgg ggagcagcgc ctcgaacatg ccgtcgcccg
    23641 caccggcgac ggcgcgggag atggatgcct gcagggcggg gcttgcgcgg aggatgacgg
    23701 cgaggagcag gggaatggag gggatgccgg tcgagacgag gctctccgtc atccggttac
    23761 gcgatgcagg gcgagcgctc tcgagcgcat cgagaagcgc cggtatggcg gggacgccga
    23821 gggcagcgag cgctcgcgcc gcgtcggcac gggtgcaggg gtcttccgca gagagggctc
    23881 gggcgaggcc ggcataatct ctctcctgct gcagcctctc aacgttcgct cgaaacctgt
    23941 caaagaatgc catgtcgccg tatccctgga aatggtcagc tgaaacgttc tatccggaga
    24001 gttctccctg agatctgagt atagtctcta tccggaacgg ataagctttc cacatccgcg
    24061 ccggccaagg attatatggt accgccctct ctctccggga ctatgttaga ggtgagactt
    24121 gatatcaccc ggctgccggg tatcgtgcgg gaactcgcgc cggatacggg cgacctcgtc
    24181 tcccggatct actcctggta cgtcgagccc ggcaacctgg tctttccgga cgccatgcag
    24241 gcgtgggtgc gcgagaagta cggcgatatc gagaggcagc agatcgtccg ggtgactaat
    24301 gaccggaccg gcgagagcac gctcttcaac tccgtccggg cacgccgccc ggttctgacg
    24361 ccccgcgccg gggagacgcg ggacctcgag gcgctggcgg agggagggcc gtttgcagat
    24421 cctctcaatg aaacgccggc cgatacgttc ggccggatcg acggcgacca ctggataacg
    24481 gcgagcaaca tcgccaggta cgattacctc cacgccgtca tcatagcaaa ggagaagaac
    24541 ccttacgtcg tcacggagcc gcaggtggcc gatatggtca gcgtggccgt ccggtggtgc
    24601 caggaggcgc accgcgccga ttcccgggca gtctatccct tcatcgtctg gaactacctc
    24661 tggcgcgcgg gcgcgagcat cgtgcacaac catgcccagg tcctcctcac ccaccgcccc
    24721 tacgccgccc cggcacgtct cgagcaggcc cgggaggagt accggtgccg ctacgggtgc
    24781 gagtattacg acgacctctt ctctgcccat gtcgccatcg gcctcggcct tgcctaccgg
    24841 ggcgcccggg tcatggcata cctcacgccg agaaaagagc acgaggtcgc gatcatcacc
    24901 gagtcgccgc acgatatcgc cccggcactt gctaaagtgc tcgcgtgtta ccgtaacata
    24961 ggggtgcaga gctacaacgc ggcggtgtat atgccgccgc ttggcgagga gggtcattac
    25021 gtgacgtggg tggtcgaccg gggcgacctt cactcccgga cgtcggatat cggggggatg
    25081 gagctctacg cggggacgcc cgtcgtcacg tcggacccgt tccgcctgat ggagcacctt
    25141 gccgccgtca tgctgccgtg agtcagcaga ggagggtttc gcgggccttc cggtacgagt
    25201 tctccatccg ctctcgaaca cggctccacg ggaactcctc cattaccttg tcaaacccct
    25261 tccggcccat cgcgagcgag agcgccggtg agtccgacgc aagggagagc ccccgggcga
    25321 tcgagtccgg gcgcggccgc accttgaccc cgtcggtccc gtgctcgatg ttctcggcaa
    25381 gcccgccgac gtcggaggcg acgacgcacc tgccggcgct ccacgcctcg aggagcacga
    25441 ggccgaacgg ctcgtttctg ctcggtatgg cgacgatatc gcttgcgttg aggagcgaga
    25501 cgtagtcggc gtcgtccagc cgcccgagga agcggacggg gagcgaccgg gcctgtgcct
    25561 caaggccccg ccgcatctgc ccgtcgccga cgaacgcaaa ctggctgtcg gcatgctcct
    25621 ggaggaggcg gggggctgcg tccaccagca tgtcggggcc tttctgccac ataagccgcc
    25681 cgacgaagag gatcagcggg gcgccggggt caaagccgta gcgctgtttc accgcctcgg
    25741 ggtcgacgtc cgcccggtaa tggccgggta tgatgccgtt ctggacgacg tcgatcttcc
    25801 agtccggtat gtcgtagagg ttcatggcct cccggcggag ggtcgacgag accgccgtca
    25861 cgcgttttgc tatctccccg ccgcgacgtt ccgccccgga tatggcgggg aacagccact
    25921 cgtcgctgta cctgttcccg ctcctcccga actccgtcga atggtaggtg aatacggtct
    25981 cgcgctcccg gagcctgacg agcgcgtcga ccacgtgcca gtcgtgaaag tgcagtagat
    26041 cgaacgccgg ccgatcgaac tcgccgaacc ggtcgagcat cttttggctc atgtcggagc
    26101 agtaacgaac gatatcttct cccgccggcc ggcagtagtg gtagtggacg ttgtttatct
    26161 ctgcatccct tccctctccc cgggtgaaga agtggacctc gtggcgctgt gccagcgtct
    26221 ccgcgaggtt ggtcgccgcg tttgcaagcc cgccgaccct ctccgcatac aatgattccc
    26281 agcaaaaaaa cgctattttc aagtgtttca tgattttcct cttagtaaat tgcccggacc
    26341 acgtggtccg gaggaccggg cggccgactc ccttaccggg tgcccggaac cgctcctggt
    26401 acaggggtcg gccggcctcc ggccgccgcg ctacatgccc gctcaaagtt gatatatctt
    26461 tggctttttt tgaaaaatgg gctgaaatga ggagttttta tagtcagggg gagaaggact
    26521 ccctcaggcg ctcttcgatg tgctcgccgt gcgagcccat ccagtaaagt ttccggcaga
    26581 cggggcacca cgagaactct ctctcccggt cctcctcgcg tgcctcgcgt gcctcgcgga
    26641 cctcgcgagc ggtggcgggc cggagatgcg tgttgcagat cgagcagcgg ttcatacgga
    26701 cccggggctc gatgagaccg aggccggcga tctggcggat ctgctccatg atatcttcgg
    26761 atacgatgta gaccgcctgc gcaccgcccc gccgcgcgag ttcgcggtcc cgggtcagga
    26821 ggagccgtcc gtcccgtgtg gcgatctcga ggaggagcgt gtcctcccgg gccatgcccg
    26881 gggctatgct gtttgcgctc atcgtgtcgt agcccatgaa ccggaggtag cgggtgagcg
    26941 tcccgagcat ccggtcggca aggaacctct tctgttcagt cgactgttga gacatactcg
    27001 atacgcccgc cgcactccgt gcaggtatgg tcgtcgatcc cccgtatgcg gactgcgtag
    27061 cccgaccgct ctatggcgag gctgccgcag gcggggcagt aggtgttctc gtaggggtgg
    27121 cccccgacgt tgccgaggta ggggtagttg agcccgagct cttttgcacg ctcgtagatc
    27181 ttctcaagtg tcctgaccgc tgtcggtctt gcgtccagca tcctgtagtc ggggtggaac
    27241 cgggtgaagt gcatcggtgt gtcggggccg aggttatcga gcacccagcg tatgagcatc
    27301 tccatctcgt ccatcgagtc gttctgtccc ggtatgacca gcgtgaccgt ctcgacgtgc
    27361 atgccgagtt cctttgcaag cgccgtcgcg tcgaggaccg gctgcagccg ccccccgcag
    27421 accttccggt agaaggggtc ggagaacgac ttgatgtcga cgcggaaggc gttgagcatg
    27481 gacgaggcct cgcggagccc ttcctcggtg atgtagccgt tcgtgacgta gacggccccg
    27541 aggcccttct cccgtgcaag cgttcccatc gcgagcgggt actcgtgcca tatggtgggt
    27601 tcgttgtagg tccaggcgat gctcgcagag cccgaccgga tggcccgctc cacgccctgc
    27661 tccggggaga ggtccctgag cgcctccatc tcgagcgtct gctgggatat gtgccagttc
    27721 tggcagtgct cgcagtggaa gttgcacccg atggtcccga ggg
//
LOCUS       DAOSII010000010        27356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_1951_length_27356_cov_47.545658, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000010 DAOSII010000000
VERSION     DAOSII010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 27356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27356
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1951_length_27356_cov_47.545658"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(255..710)
                     /locus_tag="PLV90_01565"
     CDS             complement(255..710)
                     /locus_tag="PLV90_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOF95832.1"
                     /translation="MKVEDFQKIITDAIDSEVESYTFYKSVSERVKDANLRSLFLELA
                     EDEKGHREYLQGLLLRGAKEMHFAAVSDYKVTDAIEEPALSVDMKPLDGLILAIKKEL
                     HAAQMYTRLAGASADAGQKMIFEQLANMEKGHKARLEDIYTNMAYPEAW"
     gene            1031..1762
                     /locus_tag="PLV90_01570"
     CDS             1031..1762
                     /locus_tag="PLV90_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5591 domain-containing protein"
                     /protein_id="HOF95833.1"
                     /translation="MVEDRMAGRRSAGAPRQITGVVEDRHGNLIDIFDPPFYRREFED
                     AYRFIIDDYVMAEKEIALFLPCAVRKPYSQSPSHRLFRRIIDGALDPTEYQIVIFGTC
                     GTVPAELECMYPYRNYHYMLGKTTDGRIRRDFHRIEVYRLKGFLEKTYDTYRQRLAYC
                     IGPFRKAMVEASEETGIAVDLLPSDPMIERLYDIDCPFPEGSLSMQGYIDEFREGLAR
                     LSRSVEKSSPPVIERHDRGRRPLRA"
     gene            complement(1789..3081)
                     /gene="rbcL"
                     /locus_tag="PLV90_01575"
     CDS             complement(1789..3081)
                     /gene="rbcL"
                     /locus_tag="PLV90_01575"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III ribulose-bisphosphate carboxylase"
                     /protein_id="HOF95834.1"
                     /translation="MAIDWYEEFVDTDHTPGPDELVALYYFEPAKGISREEAVGRIAS
                     ESSTGTWTTLFTMPPRMRNLQAKAFDIEGNYVKIAYPLALWEEGNAPQLLSGIAGNVF
                     GMKALERLRLIDASLPAEYIRHFKGPHFGIEGIRDMMKVRDRPLTGAVPKPKVGFTAE
                     EHAAVGYETWMGGFDFVKDDENLTSLAFNRFDDRVRAMTKLRDKAEQETGDVKSALIN
                     ITADTETMEERAKMLADYGWSYAMIDVVVTGTAAVATLRDYCSDLGLAIHAHRAMHAA
                     FDRDEKHGITMQFLAKIMRLVGVSQIHTGTAVGKLVGTRAEATVLADMLRETHMNAVD
                     HMALDQDWGGIKSAFPVSSGGLHPGLVPDVLDIYGSELVLLVSGGIHGHPKGTRAGAE
                     ATMQAIEAWQEGETLDEKAKKAPELREALDKWGYYKPR"
     assembly_gap    3631..3730
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3878..4210)
                     /locus_tag="PLV90_01580"
     CDS             complement(3878..4210)
                     /locus_tag="PLV90_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95835.1"
                     /translation="MKQDRWKLFLKALWGAVIALLVISLIAGLIGGSKFWSTMLAGSV
                     WVIIQLGALTLLLIIVVRIKEWIEKQVANVAEQKAAGTELQMLRQSMERIEEKVDRIE
                     KVLDGISE"
     gene            complement(4197..4520)
                     /locus_tag="PLV90_01585"
     CDS             complement(4197..4520)
                     /locus_tag="PLV90_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOF95836.1"
                     /translation="MLTLSFSTIPELVRLLSLLGNEQRLAILRSIAIDEKYAREIAEE
                     LGISRPLAAIYLRQLEKAGLVEGASRTSDEPPYLRRYYRAVPFEVVVDLNVVLSLERS
                     EDETG"
     gene            4955..7753
                     /locus_tag="PLV90_01590"
     CDS             4955..7753
                     /locus_tag="PLV90_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF95837.1"
                     /translation="MLHILVVDNDPALLESTRIFLERSGDLQVEVTPSPLQGLDMLTA
                     APYDAIVAGYEMPEMDGIEFLREVRGRGMGIPFIIFSGCGREEVIIEAINNGADFYLR
                     KGDNPSARLAELRNMILQGIRQRQAEAEVQRSRDMYQSIFEHTGSATIIIEADMTIAL
                     ANSEAARLTGYSREEVEGKMPWTAFVVAEDLRRLSGYHQHRRLDPGSAPRNYEFRLVD
                     REGRKKDIYMTIGSIPGTDRTVASMIDISDRKQYEANLTAAHEELTAALEEVMAGQES
                     LAMQCRELEDYQATLQGIIDFLPDPTYVLDRTGTVTIWNRAIEQVTGIQKSQIIEARP
                     EFLAEAIPGLSTSLLAASILSRKAPEGEIAREISLPSPHTGEEIDLWSKASLLYDAQG
                     GLSGAIESLRDVTELKRMEAQIRRRIDLERMVGSISARFVALDPGNLNDALNETVRTL
                     GSFFGVDRSYIFSFSADLSCAGNTHEWCAEGIEPQIDLLQNLRLDTLPWGMAHLEARR
                     AISIPDIRNLPVEAAAERELFLQYGARSLVLVPIATADEILGIVGFETVRAPRKWSDD
                     DITLLTVVGNLLANLFIRIRAHERLRESEERFRMLVERSHDCYIRATWQPPAIEYISP
                     SCERLTGYTAGECMGIPGFIPDLIHPDDREAFRTLVETPVLPADRPPILRVRRKDGRY
                     AWIEVCTIPVYGNDGHVAAVEYAVHDIDAWKEAEAALLQANKKLTLMNSIVRHDILNQ
                     VTVVLGHIALLREQPLDAAVIAALDKQQAAIETIRSQIEFTRDYQDLGVRAPQWFSVE
                     PLVTAAARALRQNRIRIVTDLNKTSVYADPLLSMVFYNLLENTVRHGKTVTTVRVTAV
                     PDGGGARITWEDDGIGIPAAHKERIFERGFGSHTGLGLFLVREILSITGMGIRETGTP
                     GRGARFEILVPEGCARFE"
     gene            complement(7856..9277)
                     /locus_tag="PLV90_01595"
     CDS             complement(7856..9277)
                     /locus_tag="PLV90_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HOF95838.1"
                     /translation="MTDKRYILALLLVMALAVPGAAAIQGGTLQTDIRCDFPGELIEA
                     GDTAVFDLAVTNRGETGTCLLNFWTFRGTDGWKIRFEAGDREVYRMLIPAGETKTVRL
                     VAETSGHAAVGEYPIRVGIGEGTIWVYVQVTKTHEGEVGTLEATVVDKEGNVVKGADV
                     GLYDGSTRVEGMLTTAEGKVSIGAPMGTYTVNVAKPGYRSWKKEGVEVRIGRTTNLGI
                     VPLEKENFFAEIRVKSPSRMAAIGTNPQYEMTLKNAGRNDDTYALSVEGLPEQWYARF
                     KESRDATEEVSEIYIPAGEEKPLYLDLIPPYSVDVGEYNFTAVIDSSARQYEENLTLR
                     LRGSYTMRTYVKSYRHEINRGDTLTFDMTVSNAGVGGALTRIGINMSAPEGWRANVDP
                     ASVASLEPGERATVRVTVVPPADIVAGEYKVVALVKSDQAEGEDEFRIVVKEQSYVAI
                     LGLLVMVGVAAGLWYMFRRYGRR"
     gene            complement(9283..10389)
                     /locus_tag="PLV90_01600"
     CDS             complement(9283..10389)
                     /locus_tag="PLV90_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOF95839.1"
                     /translation="MALDRLFNVAKKEFSDHITSRRFVIILGLLLVISAIGMHSGIES
                     YNTMLESYNQQLQYMQGAEFDGPAGWMPEKPSIMLIFSAMMTYMTTLGAVLALAIGFD
                     LVSKEKETRSLKSLLSHPVYRDEIINGKALGGIAALGFAMVLALAISLAMLLVFSIIP
                     TLEEFAAILIFGAVTLGFLLAYFAIALTMSTVSKESGNALIYTLVIFFAVSTILPTLG
                     VMAGDVFAGDRPEPPQMAPMPTPSGRTVTATASGGYFTSSSSQTVVRVGMEDPEWQQY
                     EEDMKAYTERRMLINDVSNLLSPQMNYVRASMAVTNPRIATMLSSPYSSIPEETPGLA
                     EALSQVWMNIAALIVFPSAFFAAAYVKFMRMDIR"
     gene            complement(10394..11299)
                     /locus_tag="PLV90_01605"
     CDS             complement(10394..11299)
                     /locus_tag="PLV90_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF95840.1"
                     /translation="MIRTEKLTKVYNGKTAVDGLDLTVGEGEVFGFLGPNGAGKSTTI
                     LMLTGMIEPTGGRCLVDGIEVAKDPLAVKKIIGYLPEDVGFYGNMTGEQNLDYFARFY
                     GMNARERKERITTLLELVHLDGVTQKAGEYSRGMKQRLGLAQALINDPKVLFLDEPTA
                     NLDPEGVREYRELVERLAGEGKTVFVSSHILSEVRAVCRTVGLLAKGRLVAQGTLGEV
                     ARALASPDGDAVRIVVETREHLPDIEDPDIIDIERNGNRAIVRAKADIRDRLAAALFT
                     EGAHVREMRVEEPDIEDVFMAVYRR"
     gene            11785..12792
                     /locus_tag="PLV90_01610"
     CDS             11785..12792
                     /locus_tag="PLV90_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95841.1"
                     /translation="MRRLFGAATVIVLLSLVLAAGCTSSRPPAGYVTIRSVDISIPDG
                     QPPSNVTTVVVVPYLDAVYADVGNVDLRISAKEAGTDIVVAQKVLSIGNLTNGRTEKA
                     EVTLALENGRRYDIWATVWQDGRMRESGSVSVSLPDRTTAGRRLAYSFLAVTAIDVMT
                     PDIGADPITLDIITGIKNSAAAASGDLAMDIRVVNLQTGITAVRESRPLGTVAGEGAD
                     EKQVRVTVPNGYDYEISIKLVEGGAAFATSTGRVTLAPPPQVILGNGAVLDSGKVTSP
                     VSLPVPTAAPTSPSSAQVGQFKIQSGGEPTPTQASGFTAAFAAAALLGAGAAVRAGRR
                     R"
     gene            12797..13042
                     /locus_tag="PLV90_01615"
     CDS             12797..13042
                     /locus_tag="PLV90_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HOF95842.1"
                     /translation="MPEGLTAWRAYLYVVSFIALIIVIAGSVNLIAVAIEVFAYPAPQ
                     PYPASQYYYSALPGSVAQVAVGFIVWAYHWRLLGRER"
     gene            13143..14060
                     /locus_tag="PLV90_01620"
     CDS             13143..14060
                     /locus_tag="PLV90_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOF95843.1"
                     /translation="MGAKRLNRMAMRAATERSLIDATALIQGREFESIEEANAFLQKV
                     LKEGGLPPAPADTPLLQAQQVAYDAMEATGRRRVVLARRALEISEDCADAWVILAGEE
                     RKSLRKALEYARKGVAAGERALGKEVFEEEVGNFWTLIGTRPYMRALFALAELLWATG
                     DKDEAIGLYQEMLRLNPDDRQGVRYIVMQIFAEANRDDLLESILDEHGEDPTATVAYA
                     RALWLFRKEGAGPAADAALDDALQKNPFVPQYLLAFRALPSRMPDYYGLGDRNEAIVY
                     AMAALDGWIGTEGAPAWLASRVRKMRSSR"
     gene            complement(14087..14632)
                     /locus_tag="PLV90_01625"
     CDS             complement(14087..14632)
                     /locus_tag="PLV90_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95844.1"
                     /translation="MPRRKKTVSSTPLLDSLSVDQARFVLARLIGDDPALAARAEGIA
                     RDVLRMVDTEGVAESVASDLSCIDIEEVWETSGRRRDGYVYPSERAWEMLDDALEPHK
                     GEMMAYLKRGMVEESRLCCAGILLGIRRFEQDSGSDLLEEAPDYCNETFDSVREEWEK
                     AVGDAAQVRLLARFIEEKGLS"
     gene            14909..15379
                     /gene="ruvC"
                     /locus_tag="PLV90_01630"
     CDS             14909..15379
                     /gene="ruvC"
                     /locus_tag="PLV90_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOF95845.1"
                     /translation="MTVIGIDPGVARTGYGVLKRGDPYPVPLTFGCIETGSDSRPSRR
                     LLEIYERVSLLFDEYVPDSVVLEQLFFSKNITSAMHVSEARGVILLAAEERDISITEY
                     TPNQVKQAVTGSGRADKHQVQEMVRRLLRLQEIPRPDDAADGLAIALCHINMVR"
     gene            15382..15972
                     /gene="ruvA"
                     /locus_tag="PLV90_01635"
     CDS             15382..15972
                     /gene="ruvA"
                     /locus_tag="PLV90_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOF95846.1"
                     /translation="MIDHLSGELASTGDRWVVIDIGGIGYRVQVTGPTLQDLAGTGGR
                     VMVHTHMVVRDDDIQLYGFAHPRERELFTILIGVTGIGPQTAMNILSRVSFEDFAIAI
                     INDDEKVLTRIPGIGPKSAKRLILELKDKMKKCAATLPATSHRAEAGDAVSALVSLGF
                     SGREAEEAVDAALSTLSAPTVQDLIRASLARLRERP"
     gene            15969..16964
                     /gene="ruvB"
                     /locus_tag="PLV90_01640"
     CDS             15969..16964
                     /gene="ruvB"
                     /locus_tag="PLV90_01640"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOF95847.1"
                     /translation="MKDRITSSAALREEPDDAAIRPARFDEFVGQREVKETLAIAIAA
                     AKRRGESLDHILFSGPPGLGKTTLARIIAREMEVAIRTTTGPVLDRPADLAAQLTALS
                     RGDVLFIDEIHRLNPVVEEILYPAMEDACIDVMIGEGPGARSVQLPLEEFTLVGATTK
                     VGLLGSPLRDRFGFIFRLNLYEVEDLATIVRRSAGILHTPITPEGALEIARRSRGTPR
                     IANRLLRRVRDFALVRGDGSIDEKTADLALTMLGIDRLGLDELDRRILSVIVHDFDGG
                     PVGVKTIAISIGEEVRTVEEVYEPYLIQIGFIKRTPQGRETTPAARDHIESASRG"
     gene            complement(17040..18965)
                     /locus_tag="PLV90_01645"
     CDS             complement(17040..18965)
                     /locus_tag="PLV90_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HOF95848.1"
                     /translation="MQTNSRGTETATGYRPADGTDIRQDHAKQGAASPGILTSLIVFT
                     VLLAALAATSLYNYLLFHTIAELVAVGIAIAVFTVAWNARTMRGNSYLLIAGTGLLFV
                     AGVGLIHTLAYEGMGIFSGYDANLPTQLWIASRYLLAGTLLAAPLLLRRNPAPWGVFA
                     AFTGVTGALLLSIFVYPVFPDCYVEGSGLTPFKIVSEYVVAALLALGAVAVYRLRGIF
                     SPRVAGNLIAAILVLVVSELAFTLYISVYGFANMVGHLLMVLSFGLFYVAFVETGIRQ
                     PYAVVFTGLARSEEQLRREKDRLEQYLDIAGALFVVLDRDGRITLVNRRASEILGYPA
                     NVVVGEPWIERFVPPEERERAHALFLGLMGGKSAPVYARTPVLTAGGGERIIEWHNTV
                     LRDDDGVACGTLSSGVDITERQHAEEALRQANVKLNLLSGITRHDILNQVTVARAYID
                     LTAEDSDEPGIGNYLARARTAVEEIQKQVEFTRDYQDMGVAAPVWQRPEDLINESASD
                     LALPAGVRVRTDLADLEIYADPMVKKVFYNLIDNAIRHGEKITEIRFSCAKSGRDLLL
                     LCEDDGTGIPDGEKETIFHEAYKRRHGHGLFLVTGILGITGMTIRETGIPGEGARFEI
                     RVPNGGYRGDNERCAAP"
     gene            complement(19252..21699)
                     /locus_tag="PLV90_01650"
     CDS             complement(19252..21699)
                     /locus_tag="PLV90_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF95849.1"
                     /translation="MPTQDSTAPPRDDPGATVPCRIWICGPGGEALHLPDPGWAGRIH
                     PEDMDAVFSGWKRSVTAGAPWSRRYRIQDNGGEYRTVVSSGIPVRDARGRVVLWVGVT
                     GRDKSCGEGRELATLAAIARVIEEGDADPGEVVRRVVRLLPRGFWHPEEVSVRVVPGA
                     AVPGPGKVVAGRLVVEFRDRPEGMGDPYLPQERDLIYAVAAMLEAAVAPAQGETGVSS
                     IAERRQAEEALREQQIALDNRVRELATLYAMTGIVERPGISIDEILAEVVAILPAGWL
                     HPGDAAARITLDGREYRSPGFVETCWRQESPIVVCGRIAGKVEICYLHEEPAKDEGPF
                     LLEERSLIDAVAARLGRIIERVRADEGLRRSEEKYRLLFEQMLESYTLYEVILDDAGD
                     PIDYRLAELNEKAAAVFGCSRDELIGRRLFDIFPAIREGALAIYGEVAETGIPTRRRL
                     QEPKSGRWYDLHIYRPEIGRLVVTGQEITGQKKAELALQKSEERFRGIFEQAGTGIAL
                     IDPEGRIMEANPAFVRMFGYDEDELHAMLFLDLIYPPERETARALLQKTASGESARRE
                     KRYVTKDGRILRGRLTTSLLCDPEEEGLVIGMVEDVTDRREMQNALRESEERFREIAQ
                     RSFDMIYTCYNDRGITYISPAVTRILGYTPEEMIGVHCGEYILETSRQEWLEAESRIA
                     RGEPVAGLVVGFRRKNGTAAAVEMNESPIMEGGRVIGVQVVGRDISDRKRYEDMRLQA
                     FSQIEQNIEQFAVLADHIRLPLQVILGTADLTDDTNAAEKIREQVERINRIVKQLDEG
                     WVESREIREFLRRNELV"
     gene            21953..23905
                     /locus_tag="PLV90_01655"
     CDS             21953..23905
                     /locus_tag="PLV90_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOF95850.1"
                     /translation="MAAGEACPTCPRCRAGVCEIHGPIVSRRDIAVFTIAGILLLPGI
                     LIGAFTPYPLAGTVLLLAAAVISGYDVLRAGVLSFIRLRFSIAVLISIAAAGAFLTGN
                     PAEGATVLYLYAIAEFLEEYAAGRAHRSIASLLDIAPQTARVRRDGEEVTVGVGDIGV
                     GEIVIVKPGDTIALDGIVTAGASSVDQAAITGESVPVAKGIGAGVYAGTRNVDGYLEV
                     RVTKPEAESTIARVVALVAEAQAHTSPTEAFIERFSRYYTPAVIIVAAFLVVVPPLVF
                     GIPFLEAFYRALILLVISCPCALAISTPVSMVSGITTAAHNGVLIKGRDSLEAVGLAR
                     VVVFDKTGTLTTGRLEADDLVGLDRSAEEVLAVAASLESRSGHPIAEAIRRRADEAGT
                     ALRDVGAFVSLTGRGVRGDIGGETYLLGNEGLFSGTGGAWRAAYDRLEGEGKTVVLIG
                     TDAAVIGLIALSDEIRENARSTVADIRSKGIRTVMLTGDNERVGEAVARRIGIDECHA
                     GLLPEEKVAMVERLMDRYGLVVMVGDGVNDAPALARADVGVAMGAIGSDVAIDAADIV
                     VMEDDISRVAYLVALSEKTISVVRQNVATALVVKLGIAALAVPGLVTLWMAVAFGDMG
                     LSLAVIANALRIGRPGQRFFRRMKNA"
     gene            complement(23872..24621)
                     /locus_tag="PLV90_01660"
     CDS             complement(23872..24621)
                     /locus_tag="PLV90_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOF95851.1"
                     /translation="MDTPFIFTMHEGLPRQGPGSNACTRKAFSLLAGLPPRPGILDVG
                     CGSGMQTIELARTCRDARITAVDVHQPYLDDLARRAASAGVGERVATLRASMESLPFP
                     DASFDVLWAEGSIFIMGFRKGLKSWRRLLVPGGSICITEAVWFTEEPSPEAAAFWSEC
                     YPAMTTVPENCAIAGRTGYDIVATFPLPASAWWDDYYVPLARRLDSLRAVAAGDPGAE
                     AQIEAAEREIAVFREHAGEYGYAFFILRKNR"
     gene            24738..25931
                     /locus_tag="PLV90_01665"
     CDS             24738..25931
                     /locus_tag="PLV90_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOF95852.1"
                     /translation="MEQKTPASELRDRMERFRLRMERDHPTWEFAAIFGRANQFYLTG
                     TMQDGVLLIPRDREAVLWVRRSLERALDESFFPDIRPMRSYRDAAAGMGACPGVIHVE
                     TEVVPLALLERFQKHFPCTEVRALDLQAAMVRAKKSAYELAIMERAGKIHRRVLEDAV
                     PGMLREGMSEAELGGEVFSRLVREGHHGITRFGMFGTEMVLGQLGFGENSLYPTGFDG
                     PGGCRGMAPGAPVLGSRDRTLRSGDLVFVDCGCGVQGYHTDKTVTYTFREPVPEEAVE
                     AHYRCVEVQDEAASLLRPGVMPSEIYETILGSLDPAFLENFMGYGNRRVQFLGHGIGL
                     AVDEAPAIARGFDEPLEEGMVFAIEPKKGVRGVGMVGIENTFVVTRGGGRCITGKNAG
                     LVPVE"
     gene            complement(25956..26825)
                     /locus_tag="PLV90_01670"
     CDS             complement(25956..26825)
                     /locus_tag="PLV90_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOF95853.1"
                     /translation="MPYVTVGKENSGTIDLYYEDHGAGRPVVLIHGWPLSSKSWEKQV
                     PALIDAGYRVVAYDRRGFGNSAKPAFGYDYDTLAEDLHKLMTELDLTGATLVGFSMGG
                     GEVARYLGTYGSDRVDSAVFISAIPPFLLKTSENPEGVDGSVFDGIMESIIADRPAFL
                     SGFFSEFYNVDVLGGDRISDEAVRFSWNIAATASPRGTLDSVSAWLTDFRGDLRSIDV
                     PVLVIHGDADRIVPFAASGKRIPGLIRESRLVVVEGGPHGIIWTHAGRVNRELLDFLI
                     QKAEPMQPVAGRS"
     gene            27010..>27356
                     /locus_tag="PLV90_01675"
     CDS             27010..>27356
                     /locus_tag="PLV90_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95854.1"
                     /translation="MVRKKRLLIIAATALAAFSIVFAALTVGLLYYSGSSRPSYHYSL
                     TLSTSGPIEDAVLLIPLPSRYDPDSGTNVTPVNMSLASFANFDGENISVKVEHIDGIP
                     VLNISADRIDPVYT"
BASE COUNT         4857 a         8595 c         8645 g         5159 t
ORIGIN      
        1 tgatgttctg gaggttgtgc tcccgcgccc ccttgatgac gatcttcttc attcttatgt
       61 gcagatcagt ttgtcttctt acttcatagc cgtttgccga caccaacatt taaataaaat
      121 gatggaaacg agcccggtat acaggaggtt ttgagggtaa agattgagga agcggtccga
      181 tcccgggccg tcgcgcggcc gggccgggtc cacctgcaga agagcgatag agaggggttt
      241 tgaggcggcg gtggttacca cgcctcgggg tatgccatgt tcgtgtagat atcctcgagc
      301 cgggccttgt gccccttctc catgtttgca agctgctcaa agatcatctt ctggccggca
      361 tcggcagagg cgccggcgag ccgggtgtac atctgtgcag cgtgcagttc cttcttgatg
      421 gcgaggatca ggccgtcgag cggtttcatg tcgaccgaga gcgcgggctc ctcgatagca
      481 tcggtgacct tgtaatcgct gaccgctgca aagtgcatct cctttgcacc gcggagcagc
      541 aggccctgca ggtactcccg gtggcccttc tcgtcctcgg caagttcgag gaagaggctc
      601 cgcaggttcg cgtccttcac cctctcggat acggatttgt agaaggtgta cgactcaacc
      661 tcgctgtcga tggcgtccgt aatgatcttc tggaaatctt cgactttcat agctggtttc
      721 tcctttctgc gccgtcccgg ccctgtggga ggtcggctgc acggtttgtt cgtagttata
      781 cgaacaatac tctctatgag gggccggata tttattcttt ttcttttgca tccggcagaa
      841 cctccataag ggcccggcat gggtttacgg cacccgcccg ttcctgaaaa gggggagtgc
      901 aacctgtaga gatcccggcg cgtgccgccg gcacccgggc cccggcacta ccgggatacc
      961 cccctcgcaa ggccggtgct ttttcgttcc cggcggccaa caggtacatg ctgaagagca
     1021 aacccttttc atggtggagg ataggatggc agggagacgg agcgccggtg ctccccggca
     1081 gatcaccggg gtcgtagagg acaggcacgg gaacctgatc gatatattcg atcccccctt
     1141 ctaccggagg gagtttgagg acgcttaccg gttcatcatc gacgactacg tgatggccga
     1201 gaaggagatc gctctcttcc tgccctgcgc cgtccggaaa ccctacagcc agagcccgag
     1261 ccaccggctc ttccgccgga tcatcgacgg cgccctcgat ccgacagagt accagatcgt
     1321 catattcggc acctgcggga ccgtccctgc ggagctcgag tgcatgtacc cctacagaaa
     1381 ctaccactat atgctcggca agacaacaga cgggcgaatc cggcgggatt tccaccggat
     1441 cgaggtctac cggctgaaag ggttcctcga gaagacttac gatacctacc ggcagcggct
     1501 cgcctactgc atcgggccgt tccggaaggc gatggtggag gcatcggaag agaccgggat
     1561 cgccgtcgac ctgctcccct ccgacccgat gatcgagcgg ctctacgata tcgactgccc
     1621 gtttcccgaa ggcagcctct cgatgcaggg ctatatcgac gagttccggg aggggctggc
     1681 gaggctctcg cggtcggtag agaagagcag cccgccggtc atcgagcggc acgatcgggg
     1741 tcgcagaccg ctacgggcat gacggttccg ggtttgacga aaaagtagtt atctgggttt
     1801 atagtagccc cacttatcga gggcttctct gagctcggga gccttcttcg ccttctcgtc
     1861 gagggtctcc ccctcctgcc aggcctcgat cgcctgcatg gtcgcttccg cgcccgcccg
     1921 ggtgcccttc gggtggccgt ggatgccgcc gctcacgagg aggacgagtt cgctgccgta
     1981 gatatcgagg acatccggca cgagcccggg gtggagtccg cccgacgaga cggggaaagc
     2041 gctcttgata ccgccccagt cctggtcgag tgccatgtgg tccacggcgt tcatgtgtgt
     2101 ctcccggagc atatccgcaa gcaccgtcgc ctccgcccgg gtgccgacca gtttcccgac
     2161 ggcggtcccg gtgtggatct gggaaacacc gacaaggcgc atgatcttcg ccaggaactg
     2221 catcgtgatg ccgtgctttt cgtcccggtc gaaggccgcg tgcatcgccc ggtgggcgtg
     2281 gatggcaagc cctagatcgg agcagtagtc ccggagggtc gcaacggcgg cggtcccggt
     2341 gacgacgacg tcgatcatgg cgtaactcca gccgtaatcc gcgagcatct tcgcccgctc
     2401 ctccatcgtc tccgtgtcgg ccgttatgtt gatgagcgcg gacttcacgt cgccggtctc
     2461 ctgctcggcc ttatcgcgca gtttcgtcat ggcccggacg cggtcgtcga accggttgaa
     2521 cgcaagggac gtcaggttct cgtcgtcctt cacgaagtcg aatcctccca tccaggtctc
     2581 gtagccgacc gcggcgtgct cctctgcggt gaaccccacc ttcggtttcg gcaccgcccc
     2641 cgtgagcggt ctatcccgga ccttcatcat atcccgtatc ccctcgatgc cgaagtgcgg
     2701 ccccttgaag tgccggatgt attcggccgg gagcgaggcg tcgatcaggc ggaggcgttc
     2761 gagcgccttc atgccgaaga cgttcccggc gatgccgctc aagagctggg gtgcgttccc
     2821 ttcctcccag agggcgaggg ggtaggcgat cttcacgtag ttcccctcga tatcgaacgc
     2881 tttcgcctgg aggttccgca tccggggcgg catggtaaag agcgtcgtcc aggtccctgt
     2941 cgagctctcg gaggcaatcc tcccgacggc ctcctccctg ctgatccctt ttgccggttc
     3001 aaagtagtag agggctacga gttcgtccgg cccgggggta tggtctgtgt caacaaactc
     3061 ttcgtaccaa tcaatcgcca tagaatcacc tcttgaagga agggatgccc gggtagaaaa
     3121 actttccatt cgaggccggg tagagagagg caaaatcacg ttctccctgc agacaccgga
     3181 tttatccgcc ggaggccgag atgaaggcag aaggtgtggt atgggggaga ttgccgggaa
     3241 cgtgcgctct ttgactatag tgcggaaagg ccgcccgata tcgagattgc cggcaccggc
     3301 gcccgggcct ccccggcgac cccggcagaa gccggccggt caaagcgctt tgctgccatc
     3361 cttgttctct acccgggacc cggctcccgc gccgcgttcc ttgccggtat gggcgcggtc
     3421 tccctgctcg tcgatgctcc cctggcggag agaacggtgg cggcctgggg ccgtgcagtt
     3481 acggacccgg ggggaagccg tgcgggagca tatccggacg gcgctcgacc ttcgacgggg
     3541 gcttgacctc cttatagcgc agccgggcgc cgacccggaa cgaatcggac tcgtgggcca
     3601 ccatttcggg gcgctcatcg gccgccgacc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn tttgtcataa aagatagtat tattatatgc cggattcata ttactacatg
     3781 gtcaggcgtc atgcgcgatg gatccctgcc tgcgccccgt ctgccgtacc cttagaccgg
     3841 ttactgcacg ccccccatac accctcccaa tcacttttta ctccgagatt ccatcgagaa
     3901 ccttctcgat ccggtccacc ttctcctcga tccgctccat tgactgccgc agcatctgga
     3961 gctccgtgcc ggcagccttc tgctcggcga cattcgccac ctgtttctcg atccactcct
     4021 tgatccgtac cacgatgatg aggaggagcg taagggcacc gagctgtatg atgacccaga
     4081 cggacccggc aagcatggtg ctccagaact tcgaaccgcc gatcaggccg gcgataaggc
     4141 ttatcacgag cagtgcgatg acggcgcccc agagcgcctt taagaagagt ttccatctat
     4201 cctgtttcat cttccgatct ctccaatgac agaaccacgt tcaggtccac caccacctcg
     4261 aacggcaccg cccggtagta cctccggagg tacggcggtt cgtccgacgt cctgctggct
     4321 ccctccacaa gcccggcctt ttcgagctgc cgcaggtata tcgcggccaa cgggcgcgat
     4381 atgccgagct cctcggcgat ctcgcgcgcg tacttctcgt cgatcgcgat ggaccggagt
     4441 atggcgagcc gttgctcgtt cccgagcagg gagagcagtc gtaccagttc ggggatagtg
     4501 ctgaaagata gtgtcaacac gtgtttatta ttttttacat atatgaacat tatgatacgg
     4561 catcccgtgc tcccttcggg agtttggggt ttgtgagcca cctcgccccg gagggcggga
     4621 tagcatccgg ccaccgggtt gatcggaggt ggaagacacg cacgcccggg ttgcgggcga
     4681 gatcatcaaa cgcccgggcc ctcttccgac tcggagatct caaggaagag attcgcatca
     4741 tacatacgcc cgtcatcctg ccggcggcat agtgatgccg gaacatgctg ataattcccg
     4801 ggaccggtgt cgcccgcagg tacggcaaga tctcacggat cttttatctg tgggttttaa
     4861 aatcacagtt tcttgtttct ttcgatacga acgccaatgc aaaatcacaa tatttatcgt
     4921 tgctatcgtt cagatcttca catgggatgg gttcatgctc catatccttg tggtcgacaa
     4981 tgatccggcc cttctcgaat cgacccggat ctttctggaa cgatccggag atctccaggt
     5041 tgaggtgacg ccatcgcccc tgcaggggct tgatatgctt actgcggcac cgtacgacgc
     5101 catcgttgcc ggttacgaga tgccggagat ggacggcatc gaattcttgc gagaggtccg
     5161 cgggcgcggc atgggtatac cgttcatcat attttccggc tgcgggcgcg aggaagtcat
     5221 catcgaggcg atcaacaacg gcgcggactt ctacctccgg aagggcgaca acccctcagc
     5281 ccggttagcc gagctccgga acatgatcct ccaggggatc aggcagaggc aggccgaggc
     5341 ggaggtgcag cggtcgaggg acatgtacca gagcatcttc gagcacacgg gctccgccac
     5401 catcatcatc gaggccgaca tgacgatcgc cctcgcgaac agcgaggctg ctcgccttac
     5461 cggctactcc agggaggagg ttgaagggaa gatgccctgg acggcgtttg tcgttgcaga
     5521 ggacctccgg cgcctttccg gctaccacca gcatcggcgg ttggaccccg gctccgcacc
     5581 gagaaactac gagttccggc tggtcgaccg ggaaggtaga aagaaagaca tatacatgac
     5641 gatcgggagc atcccgggaa cggatcgcac cgtcgcatcg atgatcgata tatccgaccg
     5701 aaagcagtac gaggcaaacc tgactgcggc gcatgaagag ttgactgcgg cgctcgagga
     5761 ggtgatggca ggccaggagt cgcttgcgat gcagtgccga gagctcgagg attaccaggc
     5821 aaccctccag ggaatcatcg atttcctccc cgacccgacg tatgtgctcg accgtacggg
     5881 gacagtgacc atctggaacc gggcaatcga gcaggtgacc ggtattcaga agagccagat
     5941 catcgaagca aggccggagt ttctggccga ggcgataccc ggactctcta cttcactgct
     6001 tgcggcgagc atcctctcca gaaaggcccc ggaaggggag atcgcccggg agatctctct
     6061 cccttcacct cacaccggtg aggagatcga tctctggagc aaggcatccc tgctctatga
     6121 tgcgcagggg gggctctccg gcgccatcga gtcgctccgg gatgtcaccg agctgaagag
     6181 gatggaggcg cagatccggc gccggataga cctcgagcgg atggtgggtt cgatctcggc
     6241 gcggttcgtg gccctcgacc ccggcaacct gaacgatgcc ctgaacgaga cagtccggac
     6301 acttgggtcg ttcttcggcg tcgaccggag ttacatcttc agcttctccg cggacctctc
     6361 ctgcgcaggg aacacccacg aatggtgcgc ggaggggatc gaaccgcaga tagacctgct
     6421 gcagaacctg cgcctcgata cactcccctg gggaatggcg cacctcgagg cacggagagc
     6481 gatctccata cccgacatac gcaacctccc ggtggaagcc gccgctgagc gtgaactttt
     6541 cctgcagtat ggggccagat cgcttgtcct tgtcccgatc gccaccgccg atgagattct
     6601 cggcatcgtc ggcttcgaaa cggttcgagc accccggaag tggtcggacg acgatatcac
     6661 cctgctcacg gtcgtcggca acctgcttgc caatctcttt atccggatcc gcgctcacga
     6721 acgactccgg gagagcgagg agcggttccg tatgctcgtc gagcggtcac acgactgcta
     6781 catccgtgca acctggcagc cgccggcgat cgagtatatc agcccctcgt gcgaacgcct
     6841 gacgggttac acggccgggg agtgtatggg cattccgggg ttcatcccgg atctcatcca
     6901 tccggatgat cgggaggcat tccgtaccct ggtggaaacc ccggtgctgc ctgccgatcg
     6961 gccgcccatc ctccgggtgc gccggaaaga cggacgctac gcctggatcg aggtctgcac
     7021 gatcccggtc tatgggaatg acgggcacgt agctgcagtc gagtatgccg tccacgatat
     7081 cgatgcgtgg aaagaggccg aagcagcgct cctccaggcc aacaagaaac tcacccttat
     7141 gaacagcatc gtgcggcacg acatcctgaa tcaggtcacg gtggtgctcg ggcatatcgc
     7201 cctcctccgg gagcaaccgc ttgatgcggc cgtcatcgcg gccctcgaca agcaacaggc
     7261 cgccatcgag actatacggt cgcagatcga gttcacgcgg gattatcagg acctcggtgt
     7321 ccgggccccg cagtggttct ccgtcgagcc cctggtcacg gcggcagcac gggcgctccg
     7381 gcagaaccgg atccggatcg tcaccgatct caacaagacc tccgtctacg ccgatccgct
     7441 cctctcgatg gtcttttaca acctcctcga gaacaccgtg cggcacggga agaccgtgac
     7501 caccgtccgg gtcacggccg tgcccgacgg cggcggggca cggatcacct gggaggatga
     7561 cggcatcggc atccccgccg cacataaaga gcgcatattc gagcggggtt ttgggagcca
     7621 cacgggtctt gggctcttcc ttgtgcggga gatcctctcg atcaccggga tgggcatccg
     7681 ggagaccggg acgccgggca ggggggcacg cttcgagatc ctggtgccgg aaggatgtgc
     7741 ccggttcgag tgaagagacc gggctctacc acgaaggatc gcgttcctgc aacctctccg
     7801 gacgctcgcg tttgtgatag ttctcgcaga aaaagttgtc caggggattt tggcctcacc
     7861 gccggccgta tctcctgaac atgtaccaga gccccgcggc gaccccgacc atcaccagga
     7921 ggccgaggat agcgacgtag gactgctctt tcaccacgat ccggaactca tcctctcctt
     7981 cggcctggtc gctcttcacc agggcgacga ccttgtactc gccggcgacg atatcggccg
     8041 gcgggaccac tgtcacccgc accgttgccc gttcgccggg ctcaaggctc gcgaccgatg
     8101 ccgggtcgac gtttgcccgc cacccttccg gcgcgctcat attgatcccg atcctcgtca
     8161 gcgctcctcc gacgccggcg tttgagaccg tcatatcgaa cgtgagggta tcgccgcggt
     8221 tgatctcgtg gcgatagctc ttcacgtagg tccgcatggt gtacgacccc cggagccgga
     8281 gcgtcaggtt ctcctcgtac tgccgggcgg aggagtcgat caccgccgtg aagttatact
     8341 ccccgacatc gaccgagtag ggcggtatga ggtcgaggta gaggggtttc tcctcgcccg
     8401 ccgggatgta gatctccgag acctcttcgg tggcatcccg gctctccttg aaccgggcat
     8461 accactgttc ggggaggccc tcgaccgaga gggcgtaggt atcgtcgttc ctgccggcgt
     8521 ttttcagggt catctcgtac tgcgggttgg tgccgatcgc cgccatccgt gacggggact
     8581 tcacccggat ctcggcaaag aagttctcct tctcaagcgg cactatcccg agattggtcg
     8641 tccggccgat ccggacctcg acgccctcct tcttccacga ccggtagccg ggtttggcga
     8701 cgttgaccgt gtaggtgccc atcggggcgc cgatggagac cttgccctcg gcggtcgtga
     8761 gcatcccttc gacccgggtg ctgccgtcgt aaaggcctac atcggcgcct ttcacgacgt
     8821 tgccttcttt gtcgaccacg gtggcctcga gggttcccac ttcaccctcg tgggtcttcg
     8881 tgacctggac atagacccag atcgtgccct cgccgatccc cacccggatg ggatactctc
     8941 ccaccgcggc atggccggag gtctccgcca ccaggcggac ggtctttgtc tccccggccg
     9001 gtatcagcat ccggtagact tcgcggtctc cggcctcgaa ccggatcttc cagccgtcgg
     9061 tgccccggaa ggtccagaag ttcaggaggc acgtcccggt ctccccccgg ttcgtgacgg
     9121 cgaggtcgaa gacagcggtg tcgcccgcct cgatcagttc gcccgggaag tcgcaccgta
     9181 tatcggtctg caacgtcccg ccctgtatgg ctgccgctcc cgggacggca agcgccatga
     9241 cgaggaggag ggccaggata tatcgtttgt ccgtcaatcg tctcacctga tatccatccg
     9301 catgaacttc acgtaggccg cggcgaagaa ggccgacggg aagacgatga gcgcggcgat
     9361 gttcatccag acctggctga gggcctcggc aagtcccggg gtctcctcgg gtatcgagct
     9421 gtagggtgaa ctgagcatcg tggcgatcct ggggttcgtg accgccatcg aggccctgac
     9481 atagttcatc tgcggcgaga ggaggttcga gacgtcgttt atgagcatcc gcctctcggt
     9541 atacgctttc atatcctctt catactgctg ccactcggga tcttccatcc cgacacgcac
     9601 tacggtctgc gacgaactgc tggtgaaata cccgccgctg gcggtagcgg tgaccgttct
     9661 cccggaaggg gtcggcatgg gggccatctg cggcggttcg ggccggtcgc ctgcaaatac
     9721 atcgccggcc atcaccccga gggtcgggag gatcgtggag acggcaaaaa agatcaccag
     9781 ggtgtatatc agggcgtttc cgctctcttt tgagaccgtc gacatcgtga gcgcgatggc
     9841 gaagtaggcg agcaaaaacc cgagcgtcac cgccccgaag atcaggatgg cggcaaactc
     9901 ctcgagcgtc gggatgatcg agaagacgag gagcatcgcg agcgatatgg cgagcgcaag
     9961 caccatggca aacccgaggg cggcgatgcc cccgagcgcc ttgccgttga tgatctcgtc
    10021 gcggtagacc ggatgcgaga ggagcgactt cagggatcga gtctccttct cctttgagac
    10081 caggtcaaac ccgatggcga gggcaaggac ggcgccgagc gtcgtcatgt aggtcatcat
    10141 ggcgctgaat atgagcatga tcgagggttt ttcgggcatc catcctgcag ggccatcgaa
    10201 ctcggccccc tgcatatact ggagctgctg gttgtaggac tcgagcatgg tgttgtagct
    10261 ctcgataccc gagtgcatgc cgatcgccga tataacgagg agcaggccga ggatgatgac
    10321 gaaccgcctg ctggtgatat ggtcggagaa ctcttttttc gctacgttga acaatcgatc
    10381 cagtgccatc gtttcacctc cggtagaccg ccatgaagac gtcctcgata tccggttcct
    10441 cgacccgcat ctcgcggaca tgcgcgcctt cggtaaagag agctgctgca agccggtccc
    10501 tgatatcggc cttagcccgg acgatcgccc ggttcccgtt tctttcgata tcgatgatat
    10561 cggggtcttc gatatcgggc aggtgctcgc gggtctcgac gacgatccgg acggcgtcgc
    10621 cgtccgggga tgcgagcgcc cgggcgacct ctccaagcgt tccctgcgcg acaagtctcc
    10681 cctttgcgag caggccgacg gtccggcaga ccgccctgac ctcggagagg atgtgcgagg
    10741 agacaaagac cgtctttcct tcgccggcaa ggcgttcgac gagttcgcgg tactcccgga
    10801 cgccctccgg gtcgaggttt gccgtcggtt cgtcgaggaa gagcactttc ggatcgttga
    10861 tcagcgcctg ggcgagcccg agccgctgct tcatcccgcg ggagtactcg ccggccttct
    10921 gcgtgacgcc gtcgaggtgg accagctcaa ggagtgtggt gatccgctcc tttcgctccc
    10981 gcgcgttcat accgtagaac cgggcgaagt agtccaggtt ctgctcgccg gtcatgttcc
    11041 cgtagaaccc gacgtcctcg gggaggtagc cgatgatctt cttcaccgcg agcggatcct
    11101 ttgcgacctc gatgccgtcg accaggcacc gtccgccggt gggctcgatc atgccggtga
    11161 gcatcaggat cgtcgtgctc ttccccgctc cgttcggacc gagaaagccg aagacctcgc
    11221 cttccccgac cgtaaggtcc aggccgtcga cggccgtctt cccgttatac accttcgtga
    11281 gtttttctgt ccgtatcatc cgttcacact ccgccggttc ggccgggcgg cgcctaatgc
    11341 agactttgat gctctcttat taacgtcttc tgtgacacgc atctacacgt cgggacggga
    11401 tcgcggtttt gaaccgctgc agaaccgcaa taatcccgaa agaaccccgc agatccgtcg
    11461 cggatcccgg aattaccggt gtgccggctc acgcgcaatg cgatctgccc acgcgcgcgc
    11521 ccccgtctgg cgtttcacat aaggggatag gcgcgaacga ggataaagca atggtaaact
    11581 acgaaaatta tcgaacctat gatcccgggg gcccgatgca gtcatctggc gaccggagtc
    11641 gccctattcc cggcgcactc ccgggctgcg ggctcatgcc atgataggtg caatcgcacc
    11701 cgtcttctcc cccgccggcc tgcgaaggga gccgtaatcc ggaaaccttt tcttctggtg
    11761 ctcaaaaggt tctcccggtg cagtatgaga aggttattcg gtgcagcaac ggtcatcgtg
    11821 ctcctgtccc tggtgcttgc ggcagggtgt acgtcgtccc ggcccccggc gggctacgtg
    11881 acgatccgga gcgtggatat cagcatcccg gacggacagc cgccatcaaa cgtcaccacc
    11941 gtcgtggtgg ttccttacct cgacgcggta tacgccgacg tcggcaacgt cgacctgcgg
    12001 atcagcgcga aggaggccgg gaccgatatc gtcgtcgcac agaaggttct ttcgatcggc
    12061 aacctcacaa acggcaggac ggagaaggcg gaggtcacgc ttgcgctcga gaacggcagg
    12121 cgttacgaca tatgggcgac ggtatggcag gacgggcgca tgcgggagtc gggttcggtg
    12181 agcgtttctc tccccgacag gacgacggcc gggcggaggt tggcctactc gttccttgcc
    12241 gtcaccgcga tcgacgtcat gacccccgat atcggtgccg acccgataac gctcgatatc
    12301 atcaccggca tcaagaacag cgccgccgcc gcatccgggg accttgccat ggatatccgg
    12361 gtcgtcaacc ttcagaccgg gatcacggcc gtccgcgagt cccgaccgct cggaacggtc
    12421 gccggggagg gagctgacga gaagcaggtg cgcgtcacgg tcccgaacgg ctacgactac
    12481 gagatcagca tcaaactcgt cgaggggggt gcggcctttg cgaccagcac cgggcgcgtc
    12541 accctggccc cgccgccgca ggtgatcctc ggcaacggtg cggtcctcga ttcaggcaag
    12601 gtcacgtcgc cggtctccct cccggtcccg accgcggcgc cgacgtcgcc gtcctccgca
    12661 caggtcgggc agtttaagat ccagagcggc ggtgagccga ctccgacaca ggcgtcagga
    12721 tttacggccg cgttcgccgc cgcagcgctc ctcggcgccg gggctgcggt gcgtgcgggg
    12781 cggaggaggt aggagggtgc cggagggcct cacggcgtgg agggcgtacc tctatgtcgt
    12841 ctcgttcatc gcgctcatca tcgtgattgc aggcagcgtg aaccttatcg cggtcgcaat
    12901 cgaggtcttc gcctacccgg cgccccagcc ctacccggca tcgcagtact actactccgc
    12961 tctgcccggg agcgtggcgc aggtcgcggt ggggttcatc gtctgggcat atcactggag
    13021 gctgcttggg agggaacgct gaaggcagta tcccgcccgg gtgccgcctg cctccatgct
    13081 cgtgcgtact ccatgcggaa cctctatccg gcatcaggga tcaatgaaca gatagactga
    13141 ccatgggagc aaagcgactg aaccgtatgg cgatgcgggc ggcgaccgag agatcgttga
    13201 tcgacgcgac tgcgctgata cagggcaggg aatttgaatc gatcgaggag gcgaacgcct
    13261 tcctccagaa ggtgctgaag gaaggtggtc tgccgcctgc ccccgcggat actcctcttc
    13321 tgcaggcgca gcaggtcgcg tacgatgcaa tggaggctac cgggcgccgc cgggtggtgc
    13381 tggccaggcg cgcacttgag atatccgagg actgtgccga cgcatgggtg atcctggccg
    13441 gagaggagag aaaaagcctg cgaaaagcgc tggagtatgc ccggaagggc gtggctgccg
    13501 gggaacgggc actcgggaag gaggtctttg aagaagaggt cgggaacttc tggacgctga
    13561 tcgggacacg tccctacatg agggccctgt ttgcacttgc agaactcctc tgggctaccg
    13621 gggataaaga cgaggcaatc ggactctacc aggagatgtt gcggctcaac cccgatgatc
    13681 gccagggggt ccggtacatc gtcatgcaga tctttgcgga ggcgaaccgt gacgacctgc
    13741 ttgagtcgat cctggatgag cacggagaag atcccacggc aacagtcgcg tacgcacgag
    13801 cgctctggct gttccggaag gagggggccg gacctgcagc tgatgcggca ctcgatgatg
    13861 cactgcagaa gaacccgttt gtcccgcagt atctgcttgc gttccgggcg ttgccgtcgc
    13921 ggatgcccga ctattacggc ctcggcgacc ggaacgaggc gatcgtatac gccatggctg
    13981 cactcgatgg atggatcgga acggagggtg cgccggcgtg gctcgcgtcg cgcgtgcgaa
    14041 agatgcggag cagcaggtaa ccttcctgca aggctcgggc gtgttttcac gagagccctt
    14101 tctcctcgat gaaacgggca agcaggcgca cctgcgcggc atcgccgacg gccttctccc
    14161 actcttcccg gacggagtcg aaggtctcgt tgcagtagtc gggcgcttct tcgaggaggt
    14221 ctgaccctga atcctgttca aaccgccgga tcccgaggag gatgcccgcg cagcagaggc
    14281 gtgactcttc aaccataccc cgcttgaggt atgccatcat ctcgcccttg tgcggttcaa
    14341 gcgcatcgtc cagcatctcc cacgcccgtt cggatgggta gacgtagccg tcgcgcctcc
    14401 tgcccgaggt ttcccagacc tcttctatat cgatgcagga gaggtcgctt gcgacgcttt
    14461 cggcaacacc ttcggtatcg accatccgga ggacgtcgcg tgcgatacct tccgccctgg
    14521 ccgcgagagc ggggtcatcg ccgatgagcc gtgcaaggac gaaccgcgcc tgatcgacgc
    14581 tgagggagtc gaggaggggg gtggaggaga cagttttctt tcgtctcggc atgctgatgg
    14641 taggtgtgcc ggaggagata gggttatcgt tatggtcgca gagggtgata cggctccggt
    14701 agagagcccg gcccggggct tctccgggat cgaaccttca tccctgcagg ccggctgcgc
    14761 ctcttcccgg ccatgctttc cgcagcctgc agggatggaa gggcgcagcg ttcccgacga
    14821 cggcaccccg ggtcaaccgg tgcccggcag ccggaacccc gcgaaatagg tatctagcag
    14881 gagaacggag tactcatgcg tgagggagat gaccgtgatc gggatcgatc ccggggtggc
    14941 gaggaccggc tacggtgtcc ttaagagagg cgacccctac ccggttcccc tgacattcgg
    15001 ctgcatcgag accggaagcg atagccgccc ctcccggcgg ctcctggaga tctacgagag
    15061 ggtctcgctg ctctttgacg agtacgtacc agactctgtc gtccttgagc agctcttctt
    15121 ctcaaagaac atcacctctg ccatgcacgt cagcgaagcg cgcggcgtca tcctccttgc
    15181 tgcggaagaa agagatatct cgatcaccga gtacacgccg aaccaggtca agcaggcggt
    15241 gaccgggtcg gggcgtgccg acaaacacca ggtgcaggag atggtgcggc ggcttctccg
    15301 cctgcaggag attccccggc cggacgatgc cgcggatggc ctcgccatcg ccctctgcca
    15361 catcaatatg gtgcggtaac catgatagat catctttccg gagaactggc atccaccgga
    15421 gaccggtggg tggtgatcga tatcggcggc atcggctacc gggtgcaggt caccgggccc
    15481 acgctgcagg acctcgcagg aactgggggc cgcgtcatgg tgcacaccca tatggtggtc
    15541 cgtgacgacg acatccagct ctacggattt gcccacccgc gcgagaggga actcttcacg
    15601 atcctgatcg gcgtcaccgg gatcggcccc cagaccgcca tgaacatact ctcgcgggta
    15661 tcgtttgaag acttcgcgat agcgataata aacgacgacg agaaggtcct cacccggata
    15721 ccgggcatcg ggccaaagag cgccaaacgc ctgatcctgg agctgaaaga caagatgaag
    15781 aagtgcgcgg cgacgctgcc cgccacctcc catcgggcgg aggccggcga tgcggtgagc
    15841 gccctggtat cgctcgggtt ctccgggcgc gaagcggagg aggcggtcga cgccgccctc
    15901 tccaccctct ccgctccgac ggtgcaggac ctgatccgcg cctcccttgc ccgcctgcgg
    15961 gagcgaccat gaaggaccgg atcacgtcgt ccgccgccct gcgggaggag ccggacgacg
    16021 ccgctatcag gccggcgcgg ttcgatgagt tcgtgggcca gcgcgaggta aaagagaccc
    16081 ttgccatcgc catagcagcc gcaaagaggc gcggcgaatc cctggaccat atcctcttct
    16141 ccgggccgcc gggtcttgga aagacgacgc ttgcccgtat catcgcccgg gagatggagg
    16201 tggcgatccg gaccaccacc ggcccggtgc tcgaccggcc ggccgacctt gccgcccagc
    16261 tgacggcgct ctcccggggc gacgtcctct tcatcgacga gatccaccgc ttgaatccgg
    16321 tggtcgagga gatcctgtac ccggcgatgg aggacgcctg catcgacgtg atgatcgggg
    16381 aggggccggg ggcgcggtcc gtgcagctcc cgctcgagga gttcaccctc gtcggcgcga
    16441 cgacgaaggt cggtctgctc ggatccccgc tcagggaccg gtttggtttc atattccggc
    16501 tcaacctcta cgaggtcgag gacctggcga cgatcgtccg ccggagcgcc ggtatcctgc
    16561 ataccccgat caccccggaa ggcgcgctcg agatagcgag gagaagccgc ggaacgccca
    16621 ggatagcaaa ccggctgctc cggcgggtgc gcgactttgc cctggtcagg ggcgacggga
    16681 gcatcgatga gaagaccgcc gaccttgccc ttaccatgct cggcatcgac cggttgggcc
    16741 tggacgagct cgaccggcgt atcctctcgg tgatcgtaca cgatttcgac ggcgggccgg
    16801 tgggcgtcaa gacgatcgcg atatcgatcg gagaagaggt gcggacggtc gaggaggtct
    16861 acgagcccta cctgatccag atcgggttca taaaacggac accccagggc cgggagacga
    16921 cgccggctgc acgggaccat atcgagtcgg cgtcccgcgg gtgacggccc ttcgccccca
    16981 tctccggcgg gcctgatccc cttcttgaac cgataccgat cgaccggttg cccgccggtc
    17041 tacggggccg cgcatctctc gttatcaccc cggtacccgc cgttcggcac ccttatctca
    17101 aaacgcgccc cttcaccggg gatccccgtc tcccggattg tcataccggt gataccgagg
    17161 attcccgtga ccaggaagag cccgtgcccg tgccgccgct tatacgcctc gtgaaatatt
    17221 gtctctttct cgccgtccgg gatgccggtg ccgtcgtcct cgcagaggag cagcagatcc
    17281 cgtccggatt tcgcgcagga gaaccggatc tccgttatct tctcaccatg gcgaatggcg
    17341 ttgtcgatga ggttgtaaaa gaccttcttc accatcgggt ccgcgtagat ctcaagatcc
    17401 gcaaggtccg tccggacccg aactcccgcc gggagggcga ggtctgaagc gctctcgttg
    17461 atgagatcct ccggtctctg ccagacgggc gccgcgacgc ccatatcctg gtaatcccgg
    17521 gtgaactcga cctgtttctg gatctcctcc actgcggtcc gggcacgcgc gaggtagttc
    17581 ccgataccgg gctcatcgct atcttccgcg gtgagatcga tgtaggcgcg tgcgacggtg
    17641 acctggttga gtatgtcgtg gcgggtgatc ccggagagga ggttcaactt cacgtttgcc
    17701 tgtcggagcg cctcctcagc gtgctgccgc tcggtgatat ccaccccgga actgagcgtc
    17761 ccgcaggcga cgccgtcatc gtcccggagc acggtgttgt gccactcgat gatccgctcg
    17821 ccgccgcctg cggtgaggac cggcgtcctc gcgtatacgg gcgcggattt tccccccatc
    17881 agcccgagaa agagcgcgtg ggcacgctcc cgctcctccg gagggacgaa ccgctcgatc
    17941 cagggctcgc ccacgactac attcgccgga taaccgagaa tctcggacgc cctgcggttg
    18001 acgagggtga tccggccatc ccggtcgagg acgacgaaga gggcgccggc gatatccagg
    18061 tattgctcga ggcggtcttt ctctcgccgg agctgctcct cgcttctcgc caggccggtg
    18121 aagaccaccg cgtagggttg ccggatgccg gtctcgacga atgctacgta aaagagcccg
    18181 aacgagagca ccataaggag gtggccgacc atattcgcga acccgtagac gctgatgtat
    18241 aacgtgaagg caagttccga tacgaccagc acgaggatcg ccgcgataag attgcccgcc
    18301 acccggggcg agaagatacc gcgcaaccgg tagacggcga cggcgccgag ggcgagcagg
    18361 gccgcgacga catactcgct cacgatcttg aagggcgtca accccgagcc ctcgacgtag
    18421 caatcgggaa agaccggata cacgaagatc gagaggagaa gggccccggt gactcccgta
    18481 aacgcggcaa acacccccca gggggccggg ttcctccgga gcaggagcgg cgcggcgagg
    18541 agtgtcccgg caaggaggta gcgggaggcg atccagagct gcgtcgggag gtttgcatca
    18601 tagccggaaa agattcccat cccctcatag gcaagggtgt ggatcagccc aactccggca
    18661 acgaagagga gaccggtgcc ggcgatgagc aggtaactgt tcccccgcat cgtccgggcg
    18721 ttccaggcga cggtgaagac cgctatcgct attccgacgg cgacaagttc ggcgatcgtg
    18781 tggaagagga ggtagttgta gaggctggtc gctgcgaggg cggcgagaag caccgtaaag
    18841 acgatgagcg acgtcagtat gccggggctc gctgcaccct gttttgcatg atcctgccgg
    18901 atatccgtgc cgtctgcggg gcgatacccg gttgcggtct ctgttcctct ggaattggtc
    18961 tgcatggttc gagttcgggt cgggttcttc cggctgtgtg ggggggggcg ctgaaccgct
    19021 cttcaggtgc tcttttccgg atctcatcac ggtcgcacgg ttatgcgggc gcggcaatgg
    19081 ccggcatgtt aaccctaccc gtatctgcag tacagtatat tcaattgacc gataactgtt
    19141 ctgctgcccg atgcaggagt acgggatatc gttcgacgaa agaacgtgct gcctgaagcc
    19201 gccggggtac gatccgctcc ggcagatccc gcgaccggga gatggtggcc gttataccag
    19261 ttcgtttctc cgcaggaact cccggatctc gcgggactcg acccagccct cgtcaagctg
    19321 tttcacgatc ctgttgatcc gctccacctg ttcgcggatc ttctccgccg catttgtgtc
    19381 atcggtcagg tctgccgtgc cgaggatcac ctgcaggggc aggcggatat ggtccgcgag
    19441 gactgcaaac tgctcgatgt tctgctcgat ctgggagaat gcctgcagcc gcatgtcttc
    19501 gtagcgcttc cggtccgaga tgtcccggcc gacgacctgg acgccgatca ccctccctcc
    19561 ctccatgatc ggggattcgt tcatctcgac ggcggcggcc gtcccgtttt tccggcggaa
    19621 tcccactacc agccccgcga caggctctcc ccgggcaatc cgggactcgg cctccagcca
    19681 ttcctggcgg ctcgtttcaa ggatgtactc gccgcaatgc accccgatca tctcttccgg
    19741 ggtataaccg aggatcctcg tgacagccgg ggatatgtag gtgatccccc ggtcgttgta
    19801 gcaggtgtag atcatatcga aactgcgctg ggcgatctcc cggaaccgct cctcgctctc
    19861 ccggagtgcg ttctgcatct ccctccggtc ggtgacatcc tccaccatcc cgatgacgag
    19921 cccctcctct tccggatcgc agagcagcga cgtggtcagg cggccccgca ggatcctgcc
    19981 gtcttttgtc acgtaacgct tctcccggcg ggcgctctcc ccggaggccg tcttctgcag
    20041 aagggccctg gccgtctccc tctcgggcgg atagatcagg tctaaaaaga gcatggcatg
    20101 aagttcgtcc tcgtcatagc cgaacatacg cacaaacgcc gggtttgcct ccatgatcct
    20161 cccctcggga tcgatgagcg cgatcccggt tcccgcctgt tcgaatatcc cccggaatct
    20221 ctcctcgctc ttctggaggg cgagctcggc cttcttctgt ccggtgatct cctgtcccgt
    20281 aacgacgagc ctcccgatct ccgggcggta gatgtgcagg tcgtaccagc gtccggattt
    20341 cggttcctgc aaccgccgcc gggtcgggat gcccgtctcc gcgacctccc cgtagatggc
    20401 aagggccccc tcgcggatcg ccgggaatat gtcgaagagc cgtcgcccga tcagttcgtc
    20461 gcggctacat ccgaagacgg cggcggcttt ctcgttcagt tcggcgaggc ggtaatcgat
    20521 gggatctcct gcgtcgtcca ggatcacttc gtagagcgtg taactctcga gcatctgctc
    20581 aaagaggagg cggtacttct cctcgctcct tctaaggccc tcgtcggccc gcacccgctc
    20641 gatgatccgc ccgagccttg ccgcgacggc atcgatcagg gagcgttctt cgagaaggaa
    20701 cggtccctcg tccttcgccg gttcttcgtg aagatagcag atctccacct ttcccgcgat
    20761 ccggccgcat acgacgatcg ggctctcctg cctccagcag gtctccacga acccgggaga
    20821 gcgatactcc cggccgtcga gcgtgatccg cgcggcggca tccccggggt ggagccagcc
    20881 ggcggggagg atggcgacga cctccgcgag gatctcgtcg atcgatatac cgggccgctc
    20941 gacgatgccg gtcatggcat agagggtcgc aagttccctc acccggttgt caagagcgat
    21001 ctgctgctcc ctgagcgctt cttctgcctg ccgccgttct gcgatgcttg agaccccggt
    21061 ctccccttgc gccggcgcga cggcggcctc aagcatcgcg gcgacggcgt agatgaggtc
    21121 acgctcctgc gggaggtatg gatctcccat cccctccggt cggtcccgga actcgaccac
    21181 aagacgcccg gcaacgacct tccccgggcc cgggacggcg gccccgggca cgacccgtac
    21241 cgagacctct tccgggtgcc aaaacccccg gggaaggagg cgcaccaccc gccggacgac
    21301 ctccccggga tcggcatcgc cctcctcgat cacccgggcg attgcggcga gggtcgcaag
    21361 ttccctcccc tctccgcaag atttgtcccg gccggtgacc cccacccaga ggacgacccg
    21421 acctctggcg tccctgaccg ggataccgct gctcacaacg gtgcggtact ctccgccgtt
    21481 gtcctggata cgatacctgc ggctccacgg cgcgccggca gtaacggaac gcttccaccc
    21541 ggagaacacc gcgtccatat cttccgggtg aatcctgccc gcccatccgg ggtccgggag
    21601 gtggagcgcc tcgccgccgg gtccacagat ccagatacgg caggggacag tggctcccgg
    21661 atcatcccgg ggaggtgcgg tggaatcttg tgtaggcatc gtctgctcct gcaggaggag
    21721 aaacaggatt ggcagtcgct ttcagctgga accccggggt tcgctgcacg aaatcgctcc
    21781 ccgatcagtt ggtttgaacc cattataacg tttgtgcacg gtcaacctcg ccaccgatag
    21841 caccttttaa gttcgatatg accaagattc cgtacagtca tgagagagac ccatgtcccc
    21901 ggggttgagg ccgggggttg ccgccccatc gccggcgaga cggaggcgga ccatggccgc
    21961 cggtgaggcg tgcccgacct gtccccgctg ccgggccggg gtctgcgaga tccacgggcc
    22021 catcgtcagc cgccgggata tcgcggtctt cacaatcgca ggtatcctcc ttctcccggg
    22081 aatactcatc ggcgccttca cgccgtatcc cctcgccggg actgttctgc tgctcgccgc
    22141 tgcggtcata tccggctacg acgtcctgag ggccggagtc ctctccttca tccggctccg
    22201 gttcagcatc gccgtgctca tatccatcgc ggcggcgggt gcgttcctga ccggaaaccc
    22261 cgcagagggc gcaaccgtcc tctacctcta tgccatcgcc gagttcctgg aagagtacgc
    22321 ggccgggagg gcgcatcgct cgatagcatc gctcctcgat atcgcgccgc agacggcccg
    22381 ggtccggcgg gacggcgaag aggtgaccgt cggcgtcggc gatatcggcg tgggggagat
    22441 cgtgatcgtg aagccgggcg ataccatcgc gctcgacggt atcgtcacgg ccggcgcatc
    22501 ctcggtcgac caggcggcga tcaccgggga gagcgtcccg gtggcaaagg gtatcggggc
    22561 cggcgtctac gccgggaccc gaaacgtcga tggttacctc gaggtccggg tgacaaaacc
    22621 cgaggcggag agcacgatag cccgcgtcgt ggcgctggtc gcggaggccc aggcccacac
    22681 ctcccccacc gaagcgttca tcgagcggtt ctcccgctac tacacgccgg cggtcatcat
    22741 agtcgccgcc ttcctcgtcg tggttccgcc gctcgtcttc ggcataccgt tccttgaggc
    22801 gttctaccgc gcgctgatcc tcctcgtcat atcctgcccc tgtgcgctcg ccatatccac
    22861 cccggtctcg atggtctcgg ggatcacgac cgcggcgcat aacggcgtgc tcataaaggg
    22921 ccgggactcc cttgaggccg tggggcttgc ccgggtggtc gtcttcgaca agaccgggac
    22981 gctcacgacc ggaaggctcg aggcggacga tctcgtcggt ctcgatcggt cggcagagga
    23041 ggtgctcgcc gtcgctgcgt cgcttgagtc ccgctccggc catccgatcg cggaggcgat
    23101 acggcggcgg gcggatgagg cgggcaccgc cctccgggat gtcggggcgt tcgtctcgct
    23161 gacgggaaga ggcgtccggg gggatatcgg cggtgagacc tacctcctcg ggaacgaagg
    23221 gcttttttcc ggaacaggag gggcatggcg ggcggcctac gaccgcctgg agggcgaggg
    23281 aaagaccgtc gtcctcatcg gcaccgacgc ggcggtcatc gggcttatcg ctctctcgga
    23341 tgagatccgg gagaacgcac gttcgacggt ggccgatatc cggtcaaagg ggatacggac
    23401 ggtgatgctc accggggaca acgagcgcgt cggggaggcg gttgcccgcc gcatcggtat
    23461 cgacgagtgc catgccggcc tcctcccgga ggagaaggtg gcgatggtcg agcgcctgat
    23521 ggaccgctac gggcttgtgg tgatggtcgg cgacggcgtg aacgacgcgc cggcgctggc
    23581 ccgggccgac gtcggtgtcg ctatgggggc catcgggtcg gatgtcgcga tcgacgcggc
    23641 cgatatcgtc gtgatggaag acgatatatc ccgggtcgcc tacctcgtgg ctctctcgga
    23701 gaagacaatc tcggtcgtca ggcagaacgt agcaacggcg ctcgtcgtga aactcggtat
    23761 cgccgccctc gccgtaccgg gcctggtcac cctctggatg gcggtggcgt tcggggatat
    23821 ggggctctcg cttgcggtta tagcgaacgc tctccggata ggccggcccg gtcagcggtt
    23881 cttccggagg atgaagaacg cgtagccgta ctcgccggcg tgctcccgga agacggcgat
    23941 ctcacgctcc gcggcctcga tctgggcctc cgcgccggga tcgccggcgg caaccgcccg
    24001 gaggctgtca aggcgtcttg cgagcggcac atagtagtca tcccaccacg cggatgcggg
    24061 aagggggaag gtcgcgacga tatcgtagcc ggtacgcccg gcgatcgcgc agttctccgg
    24121 gaccgtcgtc atcgccgggt aacactcgct ccagaacgcc gccgcctccg gcgagggctc
    24181 ttcggtgaac cagacggcct cggtgatgca gatggagccg cccggtacga gaaggcgcct
    24241 ccacgatttg agccctttcc gaaatcccat gataaatatc gagccctccg cccagaggac
    24301 gtcgaacgat gcatcgggaa acgggagact ctccatcgag gcccggagcg tggcgacccg
    24361 ttcccccacc ccggccgatg ccgcccgccg ggcgagatcg tccaggtagg gctggtggac
    24421 gtcgacggcg gttatgcggg cgtcgcggca ggtccgggcc agttcgatcg tctgcatgcc
    24481 ggacccgcac ccgacatcca ggatcccggg ccggggcggg agaccggcga gcagcgagaa
    24541 ggccttcctc gtgcacgcgt tgctccccgg cccctggcgg ggcaggccct cgtgcatggt
    24601 gaagatgaag ggggtatcca tgacaaccgt gcagcgtgcg gcacgataaa ccggttggga
    24661 cggcggcgca cccccgccgc agggcgcccg ggtgctctct ctctatagtt aatatcaccg
    24721 ccgcccaacc attgcgtatg gagcaaaaga cccccgcctc cgagctccgg gaccgcatgg
    24781 aacgtttccg gcttcgtatg gagagagatc acccaacatg ggagtttgcc gccatctttg
    24841 gcagggcgaa ccagttctac ctcaccggga cgatgcagga cggagttctc ctgatccccc
    24901 gcgataggga agccgtgctc tgggtgcgcc ggagccttga gcgggcgctc gacgagtcgt
    24961 tcttccccga catccggccg atgaggagtt accgcgatgc ggcggccggg atgggcgcat
    25021 gcccgggagt gatccacgtc gagacggagg tggtgcccct cgcgctgctt gagaggttcc
    25081 aaaagcactt cccctgcacg gaggtgcgag cactcgatct gcaggcggcg atggtccggg
    25141 cgaagaagag cgcctatgag ctcgcgatca tggagcgggc ggggaagatc caccggcggg
    25201 tgctcgagga cgccgttccc ggtatgctcc gggagggtat gagcgaggcc gaactcggcg
    25261 gcgaggtctt ctcacgcctg gtccgggagg ggcaccacgg gataacccgg ttcggtatgt
    25321 tcgggacgga gatggtgctc ggacagctcg gtttcgggga gaactccctc tacccgaccg
    25381 gtttcgacgg gccgggcggg tgtcggggga tggctcccgg cgctccggtc ctcggcagcc
    25441 gtgaccggac gcttagatcg ggagatttag tcttcgtcga ctgcgggtgc ggcgtgcagg
    25501 gctaccacac cgacaagacg gtgacgtaca cgttcaggga accggttccc gaggaggcgg
    25561 tggaggccca ttaccggtgc gtggaggtcc aggacgaggc ggcttcactg ttgcggccgg
    25621 gggtcatgcc ctcggagatc tacgagacga tactcggcag tctcgatccc gcgtttcttg
    25681 agaacttcat gggctacggg aaccggcggg tgcagttcct gggccacggt atcggacttg
    25741 ccgtggacga ggcgcctgcg atcgcgcgag gtttcgatga accgctcgag gaggggatgg
    25801 tctttgcgat cgagcccaag aagggggtcc gcggagtcgg gatggtcggg atcgagaaca
    25861 ccttcgtcgt cacccgcggc gggggccggt gcatcaccgg gaagaacgcc ggcctggttc
    25921 cggtggagtg agaggggagc gggttcctcc ccggttcagg agcggccggc aaccggctgc
    25981 atcggctcgg ccttctgtat aaggaagtcc agcagctcgc ggttgacgcg cccggcatgg
    26041 gtccagatga tcccgtgcgg cccgccctcg accaccacga ggcggctttc cctgatcagc
    26101 ccggggatgc gcttccccga cgcggcgaag gggacgatcc ggtcggcatc gccgtggatc
    26161 acgaggaccg gcacgtcgat gctccgaagg tcgccccgga agtcggtcag ccacgcggag
    26221 acgctgtcaa gcgtgccccg cggcgaggcg gtggctgcga tgttccagct gaagcgaacg
    26281 gcctcgtcgc tgatacggtc gcccccgagg acgtcgacgt tgtagaactc cgagaagaat
    26341 ccggagagga atgccgggcg gtcggcgatg atgctctcca tgatcccgtc aaagacactg
    26401 ccgtccacgc cttcggggtt ttcggatgtt ttcaagagga acggggggat cgcggatatg
    26461 aagacggccg aatccacgcg gtcgctccca taggtgccga ggtagcgggc gacctcgccg
    26521 ccgcccatcg agaacccgac gagcgttgca cccgtgaggt cgagttccgt catgagtttg
    26581 tgcaggtctt ctgccagggt atcgtagtcg tagccgaatg ccggtttagc agagttgcca
    26641 aaccccctgc ggtcgtaggc gacgacgcgg tagcccgcat cgatcagtgc cggcacctgc
    26701 ttctcccagg acttactcga gagcggccag ccgtggatca ggacgaccgg tcttcctgcg
    26761 ccatggtcct cgtagtagag gtcgatggtg cccgaatttt ctttaccaac ggttacgtag
    26821 ggcataggtt agcctccttc tgcgggcccc cgataccggt catcctatat agtgttgtgg
    26881 tcaggggaga gttcgccgcg gttcaggagg gctcgcacgg caggggtgca ccggaggtgg
    26941 gtttttcaaa gaacaggaag gcataagagc agcagcagtc agatcactgg tcgataacag
    27001 gtgactcgat tggtccgtaa aaagaggtta ctcatcatcg cagcaaccgc cctggcggca
    27061 ttctcgatcg tattcgcagc gttgaccgtg gggctcctgt attactccgg gagttctcga
    27121 ccgtcgtacc actacagcct gacgctctcc acgtcggggc ccatcgagga tgcggtcctc
    27181 ctgatccccc tcccctcccg ttacgacccg gactccggga ccaacgtgac ccccgtcaat
    27241 atgtcgcttg caagcttcgc taacttcgac ggggagaaca tatcggtaaa ggtcgagcat
    27301 atcgacggca tccccgtgct gaacatatcg gccgaccgga tcgatccggt ctatac
//
LOCUS       DAOSII010000011        26426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_2047_length_26426_cov_51.848091, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000011 DAOSII010000000
VERSION     DAOSII010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 26426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26426
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2047_length_26426_cov_51.848091"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..1696)
                     /locus_tag="PLV90_01680"
     CDS             complement(11..1696)
                     /locus_tag="PLV90_01680"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOF95855.1"
                     /translation="MDEDPGRLLFIYALQNAVKHGGTPKSGAVIGVMLGKHPEFRSRA
                     RDIGPLAEDAIAKVAAISPAERKSLLLELAPELLEELSGPHERSRELPALEGAENGVV
                     MRFAPNPSGPLHLGHARASILNDYYVRRYGGRYVLRIEDTDPRRVDPEAYRMVLEDIE
                     WLGLNITDIVYQSDRLDVYYDWCRRLIELGGAYVCVCDAERFRELKLKGKACPCRERT
                     ADENLDLWQQMLDGEFYEGDATVRVKTDLAHPDPAMRDFSAMRIVNAPPHPRVDATVF
                     PLMNFSVAVDDHLLGITHVIRGKDHIANTGRQRYIFDYFGWRPPVYRHYGRMGISGVV
                     LSTSGMREGIRSGLYTGWDDIHLGTLRAIARRGIEPEAVRNAMVDIGIGETDITFSWE
                     NLYARNKELVDPKANRYFFVPDPVEVTVEGAPVHEARAALHPNDPSRGVRVLVAAGTV
                     LLPKADIEGKSMVRLKDLYNIRIAWDGDVPQVTYAGESLGDARREKAPIIQWLPADAK
                     LPCTLHTQDGDIEGFCEPLVAGERDNVVQFERVGFARVDAVDGGRVSAYFAHR"
     gene            complement(1701..2666)
                     /locus_tag="PLV90_01685"
     CDS             complement(1701..2666)
                     /locus_tag="PLV90_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOF95856.1"
                     /translation="MTTLNDYLEKNADRIDRVIHRYFGAVHGDLFRASAHLLLAGGKR
                     LRPAVVILAADAVKKGASDDLLPAALALELTHNFTLIHDDIMDGDVTRRGVPTVHTVW
                     DEPTAILAGDVLYAKAFEFICLADAKDIAKVRATRMLARTCAEICEGQSLDMAFEKRD
                     DVSETEYLDMVGKKTGVLYGASAAIGGILAGANPVQADALYQFGVNSGVAFQIQDDLI
                     DLVADTAKSGKDRASDIREGKQTLIAIKARESGIDLASYRRELTGAEIDGLIARLEDA
                     GILDEVRAAAVERAAIAKEALGVLPDSEEKRLLAEIADYFITRGN"
     gene            complement(2678..4015)
                     /locus_tag="PLV90_01690"
     CDS             complement(2678..4015)
                     /locus_tag="PLV90_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase J family beta-CASP ribonuclease"
                     /protein_id="HOF95857.1"
                     /translation="MDIEIIAVGGYNEVGRNMTAVRCGKEIVIFDMGLRLDQVMIHED
                     ADIENMHSLDLIEMKAIPDDTIMNAIEGNVKAIVCSHGHLDHIGAIPKLAHRYNAPII
                     STPYTSELIRQQIAGEQKFGVNNKLFALKAGQRYTISPHLTLEFVRSQHSIIDTVFPV
                     LHTPRGAVVYANDFKLDRTPVLGEPPDFARLRQLGKEGVLALIVESTNVSEKGRCPSE
                     KIARDLVRDTITSYEDDKSAIFVSTFSSHISRVKTIAECAHEIGRKPVLLGRSMERYS
                     SAAEQLKLVGFPESLSMFGNRRTVDRTLRRIMKTGKDKFVPIVTGHQGETGAILTRIV
                     MGDTPYKLERGDKILFSAKVIPNPMNYGQRYLVEARAKMAGVRIFDDLHVSGHAYRED
                     HYEFLHLLNPQHVIPAHGDINMTGSYAKFAEEIGYTLGNDLHILRNGQKVLIT"
     gene            complement(4043..5095)
                     /gene="fni"
                     /locus_tag="PLV90_01695"
     CDS             complement(4043..5095)
                     /gene="fni"
                     /locus_tag="PLV90_01695"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HOF95858.1"
                     /translation="MTRETATSSRKRDHLLICCEKPVEAGDAGFEDVRLVHNALTECN
                     MDAVETTTRFLGSTLASPLFIAAMTGGHPDTLEVNRRLARAADRYNLGMGVGSQRAAL
                     ENPELEDSFTIVREEAPRAFLCANLGIVQLRDHGIEWVERAVEMIDAQAIAIHVNSLQ
                     EAIQPEGDHNAEGCLDALRTLCEEFSLPVIVKETGSGISAGTARIIRGAGAAAIDIGG
                     YGGTSWAKIERLRTSDSGLADLGEAFLSWGIPTVVSLCEVRTAGGPIIATGGLRSGID
                     IAKSIALGADLGGMALPLLKPAMESEDALSGAIERIHRELCVAMFLTGSRTIRDLRHA
                     RTYITGLTRQMIENSD"
     gene            complement(5092..5856)
                     /locus_tag="PLV90_01700"
     CDS             complement(5092..5856)
                     /locus_tag="PLV90_01700"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HOF95859.1"
                     /translation="MTETVVLKLGGSVITDKSGDCAIDHARLREVARELAAARRPALV
                     LVHGAGSCGHPEARRYRINDGLTGENVPGVYETHAAVSRLNDAVVEALRDAGIEAIGI
                     HPLALALAEDGRLVSFETRHIAEMTEHGIVPVLHGDVVMDRLRGSCIVSGDQLVTRLA
                     VALASRRVGLATDVPGVLQNGSVIPRIDRSTVESLDIGESTKADVTGGMRGKIAELLA
                     LADAGIESHIFHVSKVGRFLDGTGHDGTIVTQRGAP"
     gene            complement(5849..6718)
                     /gene="mvk"
                     /locus_tag="PLV90_01705"
     CDS             complement(5849..6718)
                     /gene="mvk"
                     /locus_tag="PLV90_01705"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HOF95860.1"
                     /translation="MATWSAPGKVFLFGEHAVVYGKPGVAMAIKPRVFVTVKKSRNPT
                     HPKSPYIDECFKRMGVRGSVYVNSQLQSSSGLGSSAAVTVATLSAINEEFGIGRTRDE
                     IADIAFSIERKVQSGRASPTDTYVCTNGGMVLITGTSKRRLPPQGLQLVVGNTLVPHS
                     TAKMVELVGTLQKKHPDIADPILDAIGAVTLAALHNINNQKVLGQCMDTNNALLEALG
                     VGHPASSRLVLAARASGAYGAKITGAGGGGCIIALCPRRAKSRVAGAIEACEGKAIIT
                     TIDTDGARREKND"
     gene            complement(6719..7507)
                     /gene="amrB"
                     /locus_tag="PLV90_01710"
     CDS             complement(6719..7507)
                     /gene="amrB"
                     /locus_tag="PLV90_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOF95861.1"
                     /translation="MDMRPCSVAGMFYPAEPRHLEQLLETFFQRKAPGVTSRGIVSPH
                     AGYVYSGETSACAFSTIPPDFDGTFAVIGPSHRGYMTSASAVPWETPLGVVDVDTKFV
                     DALDIPIDEASHRGEHSIEVQMPFIKYRFPRARVAPIMMGDQGYEAAADLAEQVLRAI
                     RRTGRNVRIVASSDFSHYVPDKTARQQDLYAIDALKTLDVPEFYRRLHETGATACGYG
                     PIAAMCIACRSLGAGRGELLRYTTSGDVTGDINQVVGYAAIAVV"
     gene            complement(7536..8144)
                     /gene="rpsB"
                     /locus_tag="PLV90_01715"
     CDS             complement(7536..8144)
                     /gene="rpsB"
                     /locus_tag="PLV90_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOF95862.1"
                     /translation="MTGNELEIELKEPLVPVEEYLAAGVHIGTQQKSKDMMKFIYRVR
                     GDGLYILDIQATDDRIKTAAKFLSQYEPAKILVVTSRQYGQYPAKKFADAIGGMAVIG
                     RFIPGMLTNQRLNKYIEPDVVVVTDPIGDSQAINEAVQAGIPIVALCDTNNMTKYVDL
                     VIPTNNKGRKALSMAYYLLTKELLRLRGVATSLTVEDFETEL"
     gene            complement(8146..9333)
                     /gene="eno"
                     /locus_tag="PLV90_01720"
     CDS             complement(8146..9333)
                     /gene="eno"
                     /locus_tag="PLV90_01720"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOF95863.1"
                     /translation="MTTIEQIILRTILDSRGNETVEAEIYTDCGFGRAAAPSGASTGT
                     HEARVRPPREAVEDARKNLIPSLIGEDAHDQITFDALLRENDGTLDFSSIGANVAVAL
                     SLACAKAAASSLDIELFRYLGGAFATETPLPLGNVIGGGAHASGATSIQEFLVVPTGA
                     SGATEGVFVNAAVHRAVKKILQAQGKLSGKGDEGAWAPAISDIEAFDIIASAIDTVSD
                     ETNVEVRMGIDVAASELWDGERYRYKDAARTREDQIAYMADLVDRYNLIYVEDPLFEE
                     DFDAFADLTELVGDRCLICGDDLFVTNVERITKGIETSAANCVLIKPNQIGTLTDTFE
                     AIHLARESGMETVMSHRSGETTDATIAHLATAFGCIFLKTGVVGGERIAKLNELIRIE
                     ELI"
     gene            complement(9341..9508)
                     /locus_tag="PLV90_01725"
     CDS             complement(9341..9508)
                     /locus_tag="PLV90_01725"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit K"
                     /protein_id="HOF95864.1"
                     /translation="MESYTRYERARIIGARALQISMGAPVLVKTPNTEPLEIALEEFD
                     RGVIPITVKRK"
     gene            complement(9539..9613)
                     /locus_tag="PLV90_01730"
     tRNA            complement(9539..9613)
                     /locus_tag="PLV90_01730"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9575..9577),aa:Pro,seq:cgg)
     gene            complement(9614..9802)
                     /locus_tag="PLV90_01735"
     CDS             complement(9614..9802)
                     /locus_tag="PLV90_01735"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit N"
                     /protein_id="HOF95865.1"
                     /translation="MIPVRCFTCGKVISTAWKEFERRRDAGEDPKRILDALGLERYCC
                     RRMLLTHKEVVEDLNPYQ"
     gene            complement(9837..10238)
                     /locus_tag="PLV90_01740"
     CDS             complement(9837..10238)
                     /locus_tag="PLV90_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOF95866.1"
                     /translation="MAKIINTSGKRKTAIARATLKPGNGRVRINSVPLEVYGTELTRM
                     KIAEPLLLIPNALDGVDAAIDVSGGGVVGQAEAVRTALARGIVEWHNDPAIKDAFLAY
                     DRTLLVNDSRQKETKKPHGPGARARFQKSYR"
     gene            complement(10251..10673)
                     /locus_tag="PLV90_01745"
     CDS             complement(10251..10673)
                     /locus_tag="PLV90_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOF95867.1"
                     /translation="MVTVIDADGLLLGRMASIVAQRALAGEEIALVNVEKAIVSGNRA
                     HVLAAYYTKRDRGSREGGPFFPRRPDHIVKRTIRGMLPYKRERGIAAFKRVKTYVGVP
                     MQFAGMETESLEAAHIDRLSSPRYVTVGAISKNLGAKY"
     gene            complement(10679..11044)
                     /locus_tag="PLV90_01750"
     CDS             complement(10679..11044)
                     /locus_tag="PLV90_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18e"
                     /protein_id="HOF95868.1"
                     /translation="MKTTQNKSNPRLAALIVTLKDASRVHEAKIWREIARRLDAPRKN
                     YAEVNLSKIDRYANEGETILVPGKVLGSGALNLPVKVAALDFSEAAVSKITGANGTCM
                     TIEDLLRDNPKGSRVRILR"
     gene            complement(11041..11871)
                     /locus_tag="PLV90_01755"
     CDS             complement(11041..11871)
                     /locus_tag="PLV90_01755"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit D"
                     /protein_id="HOF95869.1"
                     /translation="MEIAFSRLDERVARFTISGVSTSFANMLRRAMVSEVPTLAIEDV
                     RIYDNTSVIFDEMLAHRLGLVPLRTDLARYAVRSECSCGGAGCPVCTATYTLSVEGPK
                     TVYSSDLISQDPDAAPAEGGIPIIDLGPEQKIVLEAHAIVGTGKEHAKWQATTACGYK
                     NYPAIAVDERCDGCGMCIDECPRDVLEAGQGKVRVVSGRQELCSLCRLCERACLAGGI
                     GTEPAIRIGTEPERFIFVVESDGSMPAREIIERALQYIQRSSDDLIDVINDMTGEGTE
                     "
     gene            complement(11871..>11990)
                     /gene="rpsK"
                     /locus_tag="PLV90_01760"
     CDS             complement(11871..>11990)
                     /gene="rpsK"
                     /locus_tag="PLV90_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOF95870.1"
                     /translation="QAAIRALARAGMRIGRIEDVTPVPHDSIRGKGGRRGRRV"
     assembly_gap    11991..12090
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12091..12415)
                     /locus_tag="PLV90_01765"
     CDS             complement(<12091..12415)
                     /locus_tag="PLV90_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOF95871.1"
                     /translation="MAEEKWGIAHIFASFNNTIITITDLSGAETVTKSSGGMVVKQDR
                     NESSPYAAMQMAIQVAQNARDKGITGVHVKVRAPGRGKQRSPGPGAQAAIRALARAGM
                     RIGRIE"
     gene            complement(12420..12968)
                     /locus_tag="PLV90_01770"
     CDS             complement(12420..12968)
                     /locus_tag="PLV90_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOF95872.1"
                     /translation="MGYPGKNYKQYATPKRRFEKTRLEDEKRLLIDYGLRNKRELWKA
                     QSVLRKYRAAARELVALRSAGLNVDEYQKKKDQLTNHLYRYGLVGEGADLGDILALKV
                     EQQLDRRLQTLVQRKGLARSPKQARQFITHGHIAIAGRRVTVPGYRVARSEEAAIAYY
                     GPSPFTNSVHPERSRIARAGVR"
     gene            complement(12980..13429)
                     /locus_tag="PLV90_01775"
     CDS             complement(12980..13429)
                     /locus_tag="PLV90_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOF95873.1"
                     /translation="MDEEEIKYFVRVRNTDLDGTKAVHIALTGIKGVGSHTSRTIAAL
                     AGVDPRAIIGKLDDESVGRIASAVDTYTEQVPAWMVNRPKDIYTGDARHLLGTDLTMA
                     NDDDVNRMRKMRSYRGIRHETGQKVRGQRTKSTGRTGTTVGVKRKKD"
     gene            complement(13464..13548)
                     /locus_tag="PLV90_01780"
     tRNA            complement(13464..13548)
                     /locus_tag="PLV90_01780"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13512..13514),aa:Ser,seq:gct)
     gene            complement(13742..14719)
                     /locus_tag="PLV90_01785"
     CDS             complement(13742..14719)
                     /locus_tag="PLV90_01785"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HOF95874.1"
                     /translation="MKPTQPVKPDRDVTTLLQAMSRTGFQGRKLGESLGVWTRMVEDP
                     DCTIFMGLSGAMIPAGMQNVLIELVRHRYVDVLVSTGANIFHDTCEHLGVRHYIGHHH
                     ADDAALFEEGIDRIYDVFAYEEEFRSVDAEIARFADEIAPFRGSSREFIRRLGGWLCT
                     RRPEGRSLIATCAEYDVPIFIPALGDSSIGIGLVMARRRGVDVDVDQLADTDEITRMV
                     EESQKTGVIYVGGGVPKNFIQQTQVIASIHEQHLGGHAYAVQYTTDAPHWGGLSGCTF
                     EEAISWGKEAPESPRVQCFCDATIALPLVASALIGSGVERARRSPRREA"
     gene            complement(14908..15852)
                     /locus_tag="PLV90_01790"
     CDS             complement(14908..15852)
                     /locus_tag="PLV90_01790"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HOF95875.1"
                     /translation="MGSWYYYPSYPPSAPRPVANGLKARSKRGAIGEQWWSQQFIKAL
                     EQMGNASRLQRGKRYARKGQVTGMEVRDGEVRAKVQGSAARPYGVTIRLRALTDAEWG
                     RVFDLMSAQAIFAAQLLMGVVPHDIEKVFSEAGVPLFPASEKDITTNCTCPDWENPCK
                     HIAAVYYLLAERFDEDPFLLFALRGQSRDEVMAALRERRTEDPEPVTPLAGSFWQGDD
                     TLAGFGIEITGSAPDAGAALKIFGEPPFTVRGNGLSTVLAPAYPAAQEYARAIAGIPG
                     EEPADEQPPGKRADDDLRSPGPAPTVPAPKTGSSRRRR"
     gene            complement(15856..18870)
                     /locus_tag="PLV90_01795"
     CDS             complement(15856..18870)
                     /locus_tag="PLV90_01795"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOF95876.1"
                     /translation="MIILHSFWADGSPGAFHVWGEDPALPRKGTPRRGRKPKKPPVLP
                     HPFAADHAALTAALDLAGEPVTVTVLLPATGQDPLPSPECEPGSVVPDRADCVTYRVP
                     AIRLPGTVPVACLADLPSGPGYGATIRFWSEAARFALGLVVRQSFVPGARGWEALIRG
                     EDHDRVARLAGALPPACRFWAAGADGSPPDPAALVTSFLDRAVHTLVTGALRERPLLQ
                     KPRGRPRKIVPFGEQWVEVLSGSRDDLAGDAEEVARFYEQLGDWLSPTIAPTPLRACF
                     RLEEPEEESEQWHLTFHLQAADDPGIVIPAGDIWKKRGDVRTSLAQRFEDPQDRLLTD
                     LGRASRIYPVLQSSLTSPRPAGVNLDTSEAYAFLSEWTPLLMDSGFGVILPAWWKSAA
                     SRPSLHLTAKPAREEEGRLGLATLVSYDWTVALGDKAISPEEFEELASLKIPLVRMHG
                     QWRSIDRDEIKKALKAFTRRYPGGTMTAGELLRIAAGADPGGPAVSDVAAEGWLRGFL
                     DRSGEGGTLEAVPQPAAFSGTLRPYQQRGLSWLSFLARNGCGACLADDMGLGKTVQVI
                     AYLLAEEERGTPATPALLICPMSIVGNWRREFARFAPGLTVAVHHGSARACGDAFARQ
                     AAGSDVTITTYPLAARDRDLLASVDWNCIVLDEAQNIKNPSTKQAQAVRSFKAPQRIA
                     LTGTPVENRLTELWSIMEFLNPGYLGSFESFHSGFAVPIERYRDPAKTGHLRELIRPF
                     VLRRMKTDRSIIGDLPEKMEMKVFCSLTAEQATLYEAVVQEMLERADEAEGIRRRGIV
                     LGGLTRLKQICDHPALFLHDTAAFEGRSGKIARLLEMLEEVLDAGERALIFTQFAEFG
                     KILEPYIRERFGTAVLYLHGGTPRQKRDEMVQRFQQPGGPPIFLLSLKAGGTGLNLTA
                     ANHVFHLDRWWNPAVENQATDRAFRIGQTRDVQVRLMIASGTLEEQIDTLIEDKKALA
                     DQIVGKGEDWIGSLSTEQLRDLLTLRRDAVED"
     gene            complement(19015..19797)
                     /locus_tag="PLV90_01800"
     CDS             complement(19015..19797)
                     /locus_tag="PLV90_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOF95877.1"
                     /translation="MSDGFQILVKDVMAKPVTIAKSAFVSEALEKMLDEGVDPLIVTN
                     NGTVIGTTSRAAIAETLGSRKTQALKATSIHVANTVEENFTSAYPDQNIDILVPLLQH
                     YKLVVVFDAEHRLIGQVTAGDLLKVLRPPGNLPDVMEPAHTIQVEERAVHLRRRMLDG
                     GINRFIVEEGNQTLGIVTETDVAKALYALKDIVEGTRQEFRIRNLLVRDIMSTPLISV
                     NADTDVSDVIDLMLKKNIGSVPITQNGRIAGVVTRNSLVQAL"
     gene            complement(19794..20633)
                     /locus_tag="PLV90_01805"
     CDS             complement(19794..20633)
                     /locus_tag="PLV90_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOF95878.1"
                     /translation="MRAVDVMSAPVYIVAPADNVAYARNLMLRYRVSRLPVMEEDELR
                     GILTKKDIAYRLRQTEPMWRRRPIDRIPVSILMAENPITVAPDTSIHDIAAMMLDRDI
                     SGLPVVDEGRVVGIVTKLDIMRSAHVRGLFARVDEVMEDAVTVSRYHSLDHVIDTIKG
                     KNDKLIVVNDNGSLAGIITESNLAFYEYLDGRMNLPRKDVTHLRKEEPGGQKRFRYVV
                     EVSAVAEDIMSRPVVTVSPGVSIQDAVGLMLEHQINSLVVVENGDIRGVLKRDDIIKE
                     VAK"
     gene            complement(20642..21589)
                     /locus_tag="PLV90_01810"
     CDS             complement(20642..21589)
                     /locus_tag="PLV90_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOF95879.1"
                     /translation="MQPNSNNAEKKPADRLLKSPGKRDRGPVNFKTKIAGYEGEIMAI
                     ATKNVVTAQRTTTIIQAVEIMTREGFRRLPLVDPGTHRLRGIVTVSDIIDFMGGGDKF
                     NLVQVKHGGNFLAAVNEELREIMTSQPVAMPVTGSIADAVDIIINRNVGGLPITDAEG
                     TLKGIVTERDVMKVLATEHSGRKVEDVMRSSVRVTSPDTPIGSVCREMVTCRFRRLPV
                     VVDDVLCGIVTATDIMSYLGKGRAFEQLTTGDSAEVMGAPVRSLLSGELYTTTPDRNI
                     HEIAVDMIKKRVGAFPVIEDSHLVGLVTEYDLVKAFAEG"
     gene            complement(21634..22389)
                     /locus_tag="PLV90_01815"
     CDS             complement(21634..22389)
                     /locus_tag="PLV90_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOF95880.1"
                     /translation="MKVAENLMVDIPTLCNGDYITKARSILRDDVFRELYVLDEKNRL
                     AGYLDISDVLRVVDTKSNVTIEGFVREAARVAPDTPLTGVGRAIMNARTNSAAVVDED
                     GVFLGGVLFSELFPILISRRDICGRVKDAMSPEPVTCAPEDPIHRIYNLIIASGFIAF
                     PVVQKKEPIGIISRRDLLRAGSVRMSVKNQADTTVDRVMTTPVITVTPDDPVATAARL
                     MVDHDVSILPVIDEKKRIVGVIDRHDVSSCLAP"
     gene            complement(22386..22949)
                     /locus_tag="PLV90_01820"
     CDS             complement(22386..22949)
                     /locus_tag="PLV90_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOF95881.1"
                     /translation="MMNNSDVIHFETRVPIREVMRSHPTTIDVGETVARAAQAMCRDE
                     VGSCIVLQNSLPIGIVTEEDINCKVVAKDMKPGNVHVDRIMSTPLITIGADRMVGDAV
                     AMMVKHRVRRLPVVENQRVIGIVTVRDILTVAAEVNELLADLIEINREEVYTMGVCDR
                     CGDMSDDLSRVDNLMLCHACREEEQLL"
     gene            23161..23802
                     /locus_tag="PLV90_01825"
     CDS             23161..23802
                     /locus_tag="PLV90_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Era-like GTP-binding protein"
                     /protein_id="HOF95882.1"
                     /translation="MTFLVRTKQKISRFLKTLFKRRTCRIGIYGPPNAGKTTLANRIV
                     RDWTGDAVGPVSEVPHETRRVRRKEDITISSQNGNSVTIDIVDTPGVTTKIDYNEFVE
                     YGIEKEEAVKRAREATEGVAEAMHWLREDIDGVIYMLDATQDPFQQVNIMLVGIIESR
                     KLPVLIVANKNDLPDASPARIRSAFPQHPVISISSLEGNNVNELYEKMTSYFG"
     gene            23808..24176
                     /locus_tag="PLV90_01830"
     CDS             23808..24176
                     /locus_tag="PLV90_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2073 domain-containing protein"
                     /protein_id="HOF95883.1"
                     /translation="MIQGVQIDLISAERLDRLTTMEKIRLILDDVMEGNIVVLEKGLA
                     PDEQSKLIEITMREIAPDGFSGIEMETYPVRDAPTGFLAKLLGGKRTEARLTVIGPAN
                     QLKTLRKEKDLISAWVSSSR"
     gene            24192..24566
                     /locus_tag="PLV90_01835"
     CDS             24192..24566
                     /locus_tag="PLV90_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing protein"
                     /protein_id="HOF95884.1"
                     /translation="MPHKCTQCGREFEDGSTKILKGCPSCGGKKFLYIREAERHDDVL
                     KEKTIEEIARETGEDVLEVREDKRRAEIEVFERIESIRILGPGSYELNLERLARSDEV
                     VVGLEKEGRYAVDIISMAKKKK"
     gene            24834..26003
                     /locus_tag="PLV90_01840"
     CDS             24834..26003
                     /locus_tag="PLV90_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOF95885.1"
                     /translation="MNPTYITSLDKVAGIGAQERAALAPVLENFAFRSNDYYLSLIDW
                     EDTKDPIRRLIIPSPDELEPWGRLDPSSEHQYTRAPGLQHKYRETALLLVSDLCGGFC
                     RYCFRKRLFIEDAREVNKDVSGGLAYIRDHPEITNVLLTGGDPLVLGTDRLRDILNHL
                     RAIRHVRIIRIGTKMLAYDPYRIINDPALVDAVRGCYDGENLVYIMAQFNHPRELTDA
                     ACRAIALLQDAGAVIMNQTPLIRGINDDPAVLSKLLGRLSFVGVNPYYVFQCRPTTGN
                     RTFAVPVEESYRIFEQARATCSGLSKQARFVMSHATGKIEVVGKTGGFTYFKYNQAAD
                     PDDIGRFMVYKSNPQAYWFDDYTDLVDEGRLGDPEEAVGDARDLALSPGAGYTLG"
     gene            complement(26103..26321)
                     /locus_tag="PLV90_01845"
     CDS             complement(26103..26321)
                     /locus_tag="PLV90_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOF95886.1"
                     /translation="MVLEYINAALDHARYEIIEDAEPYYGEVPELPGVWATGRTLEEC
                     RRNLAGVIDEWLILLLRRGLPIPPVAGT"
BASE COUNT         4489 a         8213 c         8255 g         5369 t
ORIGIN      
        1 atgaattatc ttaccggtgc gcgaaatacg cgctcacccg gccgccgtcc accgcgtcta
       61 ccctcgcaaa cccgacccgc tcgaactgga cgacgttatc cctctctcct gcgaccagcg
      121 gttcgcagaa cccttcgata tccccgtcct gggtatggag ggtgcagggg agtttcgcat
      181 ccgccggcag ccactggatg atcggggcct tctcgcgccg ggcatccccg agcgactcgc
      241 ccgcgtaagt gacctgcggc acatctccat cccacgcgat ccggatgttg tagagatcct
      301 ttagccggac catgctcttc ccctcgatat ccgccttcgg gaggaggacc gtgccggcgg
      361 cgacgaggac cctgaccccc cgggagggat cgttcgggtg gagggccgca cgggcttcgt
      421 ggaccggagc cccctccacg gtgacctcga ccgggtcggg cacgaagaag tagcggtttg
      481 ccttcgggtc gacaagctcc ttgttccggg catagaggtt ctcccacgag aacgtgatat
      541 cggtctcccc gatgccgata tcgaccatgg cgttccggac cgcctcaggc tcgatccccc
      601 ggcgcgctat cgcccggagc gtcccgaggt ggatgtcgtc ccatcccgtg taaaggccgc
      661 tccggatccc ctcgcgcatc cccgacgtcg agaggacgac ccccgatatc cccatccggc
      721 cgtagtggcg gtagaccggc ggtctccagc cgaagtaatc gaatatgtag cgctgtctcc
      781 cggtattcgc gatatggtcc ttcccgcgga tgacgtgggt gatcccgagc aggtggtcgt
      841 ccaccgcgac cgagaagttc atcagcggga agaccgtggc atcgaccctc gggtgagggg
      901 gtgcgttcac gatccgcatc gccgagaagt cccgcatcgc cgggtccggg tgggcgagat
      961 ccgtcttcac ccggacggtc gcgtcgccct cgtagaactc gccatcgagc atctgctgcc
     1021 agaggtccag gttctcatcc gccgtccgct cccggcaggg gcacgccttc cccttgagct
     1081 tgagttctct gaaccgctcg gcgtcgcaga cacagacgta ggcgcccccg agctcgatga
     1141 gcctccggca ccagtcgtag tagacgtcga gccggtcgct ctggtagacg atatcggtga
     1201 tgttgagtcc cagccactcg atatcctcaa gcaccatccg gtaggcctcg gggtcgaccc
     1261 gccgggggtc ggtatcctcg atgcggagga cgtaccggcc gccgtagcgc cggacgtagt
     1321 agtcgttcaa gatggaagcc cgcgcgtgcc cgaggtgcag cggcccgctc gggttcggcg
     1381 caaaccgcat gacgacgccg ttctccgccc cttcgagggc gggaagttcg cgggaacgct
     1441 cgtgcggccc ggagagctcc tcgaggagtt cgggggcgag ttccaagaga aggctctttc
     1501 tctccgccgg ggagatcgcc gccaccttcg cgatcgcatc ttctgcaagc ggcccgatat
     1561 cgcgagcccg gctccgaaac tcggggtgtt tcccgagcat tacgccgatg accgcaccgc
     1621 tcttcggggt gccgccgtgt ttcaccgcgt tctgcagggc gtagatgaag agcaggcgcc
     1681 cgggatcctc gtccatggta tcagttgccc cgggttatga agtagtcggc aatctccgcg
     1741 aggagacgct tctcttccga atccgggagg acaccaagcg cctcttttgc aatcgcggcc
     1801 cgttcgacgg ccgcggccct gacctcgtcg aggatgccgg catcctcgag gcgggcgatc
     1861 aggccgtcga tctccgcacc cgtaagttcc ctccggtaag aagcaagatc gataccactc
     1921 tcccgcgcct tgatcgcgat aagggtctgc ttcccttccc ggatatccga ggcacggtcc
     1981 ttgccgctct ttgcggtatc ggcaacgagg tcgatcaggt cgtcctggat ctggaaggcg
     2041 acgccgctgt tcaccccgaa ctggtagagg gcatcggcct ggacggggtt tgcacccgcg
     2101 agaatcccgc cgatggcggc cgacgcgccg tagagcacgc cggtcttctt gccgaccata
     2161 tcgaggtact ccgtctccga gacgtcgtct cgcttctcaa acgccatatc gaggctctgc
     2221 ccctcgcaga tctcggcaca cgtccgggca agcatcctcg tcgcccggac tttcgcgata
     2281 tcttttgcat ccgcaaggca gatgaactcg aacgccttcg cgtagaggac gtcgcctgca
     2341 aggatggccg tcggctcatc ccagacggtg tgcaccgtcg ggacgccgcg ccgggtgacg
     2401 tccccgtcca tgatgtcgtc atggatcagg gtgaagttgt gggtcagttc gagcgcgaga
     2461 gccgcgggga gcaggtcgtc cgaggctcct ttcttcaccg catccgcggc gaggatcacc
     2521 accgccgggc ggagcctttt gccgcctgca agcagcaggt gggcgctcgc ccggaagagg
     2581 tcgccgtgga cggctccaaa gtagcggtgg atcaccctgt cgatccggtc ggcattcttc
     2641 tccaggtaat cgttcagcgt cgtcatgcct tcatctccta cgttatcagc accttctgcc
     2701 cgttcctgag tatgtgcagg tcgtttccga gcgtataccc gatctcctcc gcaaacttcg
     2761 cgtaactgcc ggtcatgttg atgtcgccgt gagccggtat gacgtgctgg gggttcaaga
     2821 ggtgcaggaa ctcgtaatgg tcctcgcggt atgcgtgccc cgagacgtgc aggtcgtcga
     2881 atatccgcac gcctgccatc ttggcgcgtg cctcgaccag gtaacgctgc ccgtagttca
     2941 tcgggttcgg gataaccttc gccgagaaga ggatcttgtc gcccctctcc agtttgtagg
     3001 gcgtatcccc catgacgatc ctcgtcagga tggcgcccgt ctccccctgg tggccggtga
     3061 cgatcgggac gaacttatcc ttcccggtct tcatgatccg ccgcagcgtc cggtcgacgg
     3121 tccggcggtt gccgaacatc gagagcgatt cggggaaccc gaccagtttc aactgctcgg
     3181 ccgccgagct gtagcgctcc atcgaccgcc cgaggaggac cggctttctg ccgatctcgt
     3241 gggcgcactc ggcgatggtc ttgacacggg agatgtgcga cgagaacgtc gagacgaata
     3301 tggcgctctt gtcgtcctcg taactcgtta tggtatcccg gacaaggtcc cgcgctatct
     3361 tctcgctcgg gcatcgccct ttctcgctga cgttcgtgct ctcgacgatc agggcaagca
     3421 cgccctcttt gccgagctgc cgcagccgcg cgaagtccgg cggttcgccg aggaccggcg
     3481 tccggtcgag cttgaagtcg tttgcgtaga cgaccgcgcc tcgcggcgtg tgcaggacgg
     3541 gaaagaccgt atcgatgatc gagtgctggc tccgcacgaa ctcgagcgtc aggtgcgggg
     3601 agatggtgta gcgctgtccg gccttcaggg cgaagagctt attgttcacc ccgaacttct
     3661 gttcgcccgc gatctgctgc cggatcagtt ccgacgtata gggcgtgctg atgatcgggg
     3721 cgttgtagcg gtgcgccagt ttcggtatag cgccgatatg gtccaggtgc ccatgcgaac
     3781 agacgatcgc cttgacgttc ccctctattg cattcatgat ggtgtcatca ggaatggcct
     3841 tcatctcgat caggtccaga gaatgcatgt tctcgatatc cgcatcctcg tggatcatca
     3901 cctggtcaag acgcaggccc atatcaaaga tgacaatctc cttcccgcag cggacggcgg
     3961 tcatatttct gccgacttcg ttatacccgc ccactgctat gatttcaata tccatgtagc
     4021 ctccttgcag gaatttttgt agttagtcgc tgttttcaat catctgccgg gtcaatccgg
     4081 taatatacgt ccgtgcgtgc cggagatccc gtatcgtccg ggatcctgtc aggaacatcg
     4141 caacgcagag ttcgcggtgt atcctctcga tggcgccaga gagtgcgtct tcgctctcca
     4201 tggccggctt caagagaggg agcgccatgc ccccgagatc cgccccgagt gcaatggatt
     4261 tcgcgatatc gatccctgaa cgcagcccac ccgtcgcaat gatcgggcct cctgccgttc
     4321 gcacctcgca gaggcttacc acggtcggta tgccccagga gaggaacgct tccccgagat
     4381 cggcaagacc ggagtcgctc gtccgcaggc gttctatctt cgcccaggac gtcccgccgt
     4441 aaccaccgat atcgatggcg gccgctccgg ctcccctgat tatccttgcg gttccggcag
     4501 atatgccgga accggtctcc ttcacgatga ccgggaggga aaactcctca cagagggtgc
     4561 ggagtgcatc caggcacccc tctgcgttat gatcgccctc cggctgaatc gcttcctgga
     4621 gcgagttgac gtggatagcg atcgcctgtg cgtcgatcat ctcgacagcc cgctccaccc
     4681 actctatgcc gtggtcgcgg agctggacga tgccgaggtt cgcgcagagg aaggcccgcg
     4741 gcgcttcctc ccgcacgatg gtgaaactat cctccagttc gggattctcc aatgccgctc
     4801 gctgagaacc aacacccata ccgaggttgt atcggtcggc agcacgtgca agccgccggt
     4861 tcacctcaag ggtatccgga tgtcctccgg tcatcgctgc aatgaagagg ggggaggcga
     4921 gtgtgctgcc gagaaaccgc gtcgtggtct cgacggcatc catgttgcat tccgtgaggg
     4981 cgttatggac cagccggaca tcctcgaaac cggcgtcgcc ggcctcgacg ggtttttcgc
     5041 agcagatgag gaggtggtcc cgtttccttg aagatgtcgc ggtctcgcgg gtcatggtgc
     5101 gcctctctgg gttacaatcg tcccgtcgtg ccccgttccg tccaggaacc ggccgacctt
     5161 cgagacgtgg aatatgtgcg attctattcc ggcatcggcg agcgcgagga gttccgctat
     5221 cttgccccgc atgcctccgg tcacgtcggc cttcgtcgat tcgccgatat cgagcgattc
     5281 taccgtgctt cggtcgatgc gcggtatgac cgacccgttc tgcaggacgc cggggacatc
     5341 cgtcgcaaga ccgacgcgcc ggctcgcgag agccacggca agccgggtca ccagctgatc
     5401 gcccgacacg atgcaggagc cccgcaggcg gtccatcacc acgtcgccgt gcagcacggg
     5461 cacgatgccg tgttctgtca tctcggcgat gtggcgggtc tcaaacgaga ccaggcggcc
     5521 gtcttcggcg agggcgaggg cgagggggtg gatgccgatc gcctcgatgc ccgcatcccg
     5581 cagggcctcg acgaccgcat cgttcaggcg tgagacggcg gcgtgcgtct cgtagacgcc
     5641 gggcacgttc tctcccgtga gaccgtcgtt gatccggtaa cgccgggcct ccgggtgccc
     5701 gcacgagcct gcgccgtgga cgaggacgag agccggcctt ctcgccgcgg caagttccct
     5761 tgcaacctcg cggaggcgcg cgtgatcgat ggcacagtct cccgacttat cggtgatcac
     5821 gctcccgccc agtttcagta ccacggtctc agtcattctt ctctcttctc gcgccgtcgg
     5881 tatctatggt ggtgatgatg gcctttccct cgcaggcctc gatggccccg gcaacccggc
     5941 tcttagcgcg ccgggggcag agggcgatga tgcacccgcc accccctgcc ccggtgatct
     6001 ttgcgccgta ggcgccgctc gcccttgccg cgagcacaag cctgctgctt gcggggtgtc
     6061 cgacgccgag ggcctccagg agggcgttgt tcgtatccat gcactgcccg agcactttct
     6121 ggttgttgat gttgtggagg gcggcaagcg tcaccgcccc gatggcgtcc aggatcgggt
     6181 ccgcgatatc gggatgtttc ttctgcaggg tcccgaccag ttccaccatc tttgcggtac
     6241 tgtgcggcac gagcgtattc ccgacgacga gctggaggcc ctgcggcggg agccgtcgct
     6301 tggatgtccc cgtgatgagc accatcccgc cgttcgtgca gacgtaggta tcggtggggc
     6361 ttgcccgtcc gctctggact ttcctctcga tggaaaacgc gatatcggca atctcgtcac
     6421 gggtgcgccc gatcccgaac tcttcgttga ttgcagagag cgtcgccacc gtcaccgcgg
     6481 ccgaggaccc aagccccgac gaactctgga gttgcgagtt gacgtagacg ctgcctcgaa
     6541 cacccatccg tttgaagcac tcgtcgatgt agggcgattt cgggtgggtc gggttccgtg
     6601 acttcttcac ggtgacaaag acccggggtt tgatcgccat cgccaccccc ggtttaccgt
     6661 agaccactgc atgctcgccg aagaggaaga ccttgcccgg cgcgctccac gttgccaatc
     6721 agaccaccgc tatcgccgca taccccacta cctgattgat atcccccgtc acatcgccgc
     6781 ttgtcgtata ccggaggagc tcgccccgcc ctgcaccaag cgaccggcag gcgatgcaca
     6841 tggccgcgat cgggccgtag ccgcacgccg tcgcaccggt ctcgtggagc cggcggtaaa
     6901 actccggcac atccagggtc ttgagcgcat cgatcgcgta gaggtcctgc tgtcgagcgg
     6961 ttttatccgg gacgtagtgg gagaagtcgc tcgaggcgac gatccgcaca ttccttcccg
     7021 tccgccggat cgcccggagc acctgctccg caagatccgc cgccgcctcg tatccctggt
     7081 ctcccatcat gataggcgca accctcgctc gcgggaaccg gtacttgatg aacggcatct
     7141 gcacctcgat cgagtgttca ccccggtgcg acgcctcatc gatagggata tccagcgcat
     7201 cgacgaactt cgtatcgacg tcgacgaccc cgagcggcgt ctcccacggc acggcggatg
     7261 cagaggtcat gtatccccgg tggctcgggc cgatgacggc gaaggtgccg tcaaaatcag
     7321 gtggtatggt cgagaaggca caggccgagg tctcccctga gtagacgtag cctgcatggg
     7381 gggagacgat cccccgggac gtgacacccg gggccttcct ctggaagaat gtctccagca
     7441 gctgctccag atgccggggc tcggcaggat agaacattcc cgctacactg catgggcgca
     7501 tatccatcaa gattgagctc ttgtgccttc gagtcttata actctgtctc aaagtcctca
     7561 acggtgagcg atgtggcaac accgcgcagg cggagcaact cttttgtcag gaggtagtac
     7621 gccatcgaga gcgctttcct gcccttgttg ttggtgggga tgaccagatc gacgtacttc
     7681 gtcatgttat tggtgtcgca gagggcgacg atcggtatac ccgcctggac ggcttcgttg
     7741 atcgcctggg agtcgccgat ggggtcggtc accacgacca catcgggttc gatgtacttg
     7801 ttcaggcgct ggttggtgag catcccgggg atgaaccggc cgatgacggc catgccgccg
     7861 atggcgtcgg cgaacttctt cgccgggtac tggccgtact gccgggaggt gacgaccagg
     7921 atcttcgcgg gctcatactg cgaaaggaac ttcgcagcgg tcttgatgcg gtcgtcggtc
     7981 gcctggatat ccaggatgta gagtccatcc ccgcggacgc ggtagatgaa cttcatcatg
     8041 tctttgctct tctgctgggt gccgatgtgc acgcctgctg cgagatactc ctctacgggc
     8101 accagcggtt ctttcagctc gatctcgagt tcgtttcctg tcaagttaga tcagctcctc
     8161 tatgcgaatc agttcgttca gttttgctat tcgttctccg ccgacaacgc ctgtcttgag
     8221 gaatatgcac ccaaatgcgg ttgcaaggtg cgctatcgtc gcgtcggtgg tctcgccgga
     8281 ccggtggctc atgaccgtct ccatcccgct ctcccgggcg aggtggatcg cctcgaaggt
     8341 atcggtgagc gtgccgatct ggtttggttt tatcaggacg cagtttgccg cgctggtctc
     8401 gatgcctttc gtgatccgct cgacgttggt cacgaagagg tcgtcgccgc agatgaggca
     8461 gcggtccccg accagttcgg tcaggtcggc gaacgcgtcg aagtcctcct caaagagggg
     8521 atcttcgacg tagatgaggt tgtagcggtc gacgagatcg gccatgtagg cgatctggtc
     8581 ctcgcgggtc cgggcggcat ccttgtagcg gtaacgctcg ccgtcccaga gttcgcttgc
     8641 cgcgacgtcg atccccatcc ggacctcgac gttcgtctca tcagagacgg tatcgatcgc
     8701 cgaagcgatg atatcgaacg cctcgatgtc ggatatggcc ggtgcccagg caccttcatc
     8761 ccctttgccg gagagtttgc cctgcgcctg caggatcttc tttacggccc tgtgaacggc
     8821 ggcgttcacg aagacgccct ccgtcgcgcc ggaggcaccg gtgggaacca ccaggaactc
     8881 ctggatggag gtggcgcccg atgcgtgcgc gccgccgccg atgacgttcc cgagagggag
     8941 gggcgtctcg gtggcgaatg cgccgcccag gtaccggaag agttcgatgt caagagacga
     9001 tgcagccgcc ttcgcgcacg caagcgagag cgctacggcg acgtttgcgc cgattgaact
     9061 gaaatcgagt gttccgtcgt tctccctgag cagtgcgtcg aacgtgatct gatcgtgagc
     9121 gtcctcaccg atgagcgatg gaatcaggtt tttccgtgca tcctcgacag cctcgcgcgg
     9181 cggccgcacc ctggcttcgt gggtccccgt gctcgcgccg ctcggcgccg ctgcacgccc
     9241 aaaaccgcaa tccgtgtaaa tttcggcctc gaccgtctcg tttccccggc tatccaggat
     9301 cgttcttaag ataatctgtt cgatggtcgt catcagatca ctactttctc ttcaccgtta
     9361 tagggatcac gcctcgatcg aactcttcaa gtgcgatctc aagcggctcg gtatttggtg
     9421 tcttaaccag aaccggtgca cccatcgata tctgcagagc acgagcccct atgatccgcg
     9481 ctcgttcata tcgggtatat gattccatcg tgcagcctca aaatcattcg taataaaatg
     9541 gggtcgctga gatttgaact caggtcacag catcccgaac gccataggat agtccaggct
     9601 accccacgac ccctcattga tacgggttca gatcctccac tacttccttg tgcgtcagca
     9661 gcattcgcct gcagcaatac cgctccaggc cgagagcgtc gaggatccgt ttcggatcct
     9721 cgcccgcatc tcgccgcctc tcgaactcct tccaggctgt agagatgact ttaccgcatg
     9781 taaaacatcg tacgggtatc atataaattg tcctgtatcc cttatatctc ttttcctcac
     9841 cggtaggact tctggaaccg tgcccgtgca cccggaccgt gcggtttctt ggtctccttc
     9901 tgccgcgagt cgttcacgag cagcgtccgg tcgtacgcaa ggaacgcatc cttgatcgcg
     9961 gggtcgttgt gccactccac gatgccgcgt gcaagcgccg tccggaccgc ctcggcctgt
    10021 cccaccacac cgccgccgga gacgtcgatc gccgcatcga cgccgtcgag cgcgttcggg
    10081 atcagcagca ggggttcggc gatcttcatg cgcgtcagct cggtcccgta gacctccagg
    10141 ggcacggagt tgatgcggac ccggccgttg ccgggtttca gggtcgcccg ggcgatagcc
    10201 gtctttctct taccgcttgt attgataatc tttgccattc cgtcacctca tcagtacttt
    10261 gccccgaggt tcttgcttat cgccccgacc gttacatacc tcgggctgct caatcggtcg
    10321 atgtgggccg cttccaggga ttcggtctcc ataccggcaa actgcatggg aacgccgacg
    10381 tatgtcttta cccgcttgaa cgccgcgatg ccgcgctcgc gcttgtaggg aagcatcccg
    10441 cggatggtgc gcttgacgat gtggtcaggc ctgcgcggga agaacgggcc gccctcgcgg
    10501 gacccccggt cgcgcttcgt gtagtaagcc gcgagcacgt gcgcccggtt gccggagacg
    10561 atcgctttct ccacgttcac gagggcaatc tcttcgccgg cgagcgcgcg ctgcgcgacg
    10621 atgcttgcca tcctcccgag cagcagtccg tctgcatcga taactgttac cattcgtctc
    10681 acctgaggat ccgtacccgg ctccccttcg ggttgtcccg gaggaggtcc tcgatggtca
    10741 tgcacgttcc gttggcgccg gtgatcttgc tcaccgcggc ctcggagaag tccagcgcgg
    10801 ctaccttcac cggcaggttg agcgcaccgc tgccgagcac cttgcccggc accaggatcg
    10861 tctcgccttc gtttgcgtac cggtcgatct tgctgaggtt cacctcggcg tagttcttcc
    10921 ggggggcatc cagcctccgc gcaatctcac gccagatctt cgcctcgtgt acacgcgacg
    10981 catctttcag cgtcacgatg agggcggcca gccgggggtt ggatttattc tgggttgtct
    11041 tcattcagtc ccctctcccg tcatatcgtt tatcacatct atcaggtcgt ctgatgatct
    11101 ctgaatgtac tggagcgctc tctcgatgat ctcccgggcg ggcatcgacc cgtcgctctc
    11161 caccacgaag atgaatcgct ccggttcggt gccgatccgg atggccggct cggtgccgat
    11221 gccgccggca aggcacgccc gttcgcagag cctgcagagc gaacagagct cctgccgccc
    11281 gctgacgacc cggaccttgc cctgccccgc ctcgagcaca tcgcgcggac actcatcgat
    11341 gcacatgccg cacccgtcac accgctcgtc gacggcgatc gccgggtagt tcttgtaacc
    11401 gcaggcggtc gtcgcctgcc acttggcgtg ctccttcccg gtgccgacga tggcgtgcgc
    11461 ctcgagcacg atcttctgct ccggcccgag atcgatgatg gggatacctc cttccgccgg
    11521 tgcggcatcg gggtcctggg agatcaggtc gcttgagtag accgtctttg gtccctcgac
    11581 ggagagcgtg taagtggcgg tgcagaccgg gcaaccggcg cctccgcagg agcactcgct
    11641 ccggacggcg tagcgtgcaa ggtcggtccg gaggggtacg agccccagcc ggtgtgccag
    11701 catctcatcg aagatgacgc tcgtgttgtc gtatatgcgg acatcctcga ttgcgagcgt
    11761 cggcacttcg ctgaccatcg ctcgccggag catgtttgca aacgacgtcg aaacgccgct
    11821 tatggtgaat cgggcgactc tctcgtccag tcgagaaaac gcaatctcca ttacactctc
    11881 cttcctctcc ggccgccctt gccgcggatg ctgtcgtggg gcaccggagt gacatcttca
    11941 atgcggccga tcctcatccc cgcacgggcg agggcgcgga tcgctgcctg nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cttcaatgcg gccgatcctc atccccgcac
    12121 gggcgagggc gcggatcgct gcctgagcgc cgggaccggg gctcctctgt ttgccgcgtc
    12181 cgggtgcacg caccttcacg tggacgccgg tgatcccttt atcgcgcgcg ttctgggcga
    12241 cctggatcgc catctgcatg gccgcgtagg gcgaactttc gtttcggtcc tgtttcacga
    12301 ccataccgcc gctgctcttg gtgaccgtct cggcgccgga gaggtcggtg atggtgatga
    12361 tggtattgtt gaaggaggca aatatgtgtg caatgcccca tttctcttct gccatggtct
    12421 tacctcactc ctgcgcgggc gatcctgctt ctctcggggt gaacgctgtt cgtgaacggg
    12481 gaggggccgt agtaggcgat agcggcctct tcgcttcttg caacccggta gcccggcacg
    12541 gtgacccggc ggccggcgat cgcgatgtgt ccgtgggtga tgaactggcg ggcctgtttc
    12601 ggggagcgtg caagcccctt cctctggacc agcgtctgga ggcggcggtc gagctgctgc
    12661 tcgaccttca acgcaaggat gtcgccaagg tcggcgccct cgccgacaag accgtagcga
    12721 tagaggtggt tggtgagctg gtccttcttc ttctggtact catccacgtt gaggcctgca
    12781 gaccgcagcg ccacgagctc gcgggcggca gcccggtact tgcgcaggac gctctgggcc
    12841 ttccagagtt cacgcttgtt ccggagcccg tagtcgatga ggagcctctt ttcgtcttca
    12901 agtctcgtct tctcgaaccg ccgcttgggc gtcgcgtact gcttgtagtt ctttcccgga
    12961 tatcccattc aatcaccgtt cagtccttct ttctcttgac gccgacggtc gtgccggttc
    13021 ttccggtgga cttggtgcgc tggccgcgga ccttctgccc cgtctcgtgc cttatgccgc
    13081 ggtagctccg catcttcctc atgcggttga cgtcgtcgtc gtttgccatg gtgaggtcgg
    13141 tcccgaggag atggcgggca tctcccgtgt aaatgtcttt tggacggttc accatccatg
    13201 caggcacctg ctcggtgtag gtgtcgacgg cgcttgcaat acgcccgacg gattcgtcgt
    13261 ccagcttgcc gatgatggcg cggggatcca caccggcaag ggcggcgatg gtgcgggacg
    13321 tgtgcgaacc gacgcctttg ataccggtca gtgcgatgtg cacggccttg gtgccgtcga
    13381 gatcagtgtt cctgactcga acaaagtact ttatctcttc ttcatccatg tgatcgcctc
    13441 ttcatcgacg ttgtcggtga aagcgctgag ggagggattt gaaccctcga gtcccagggg
    13501 gacacaggtt tagcaaacct gcgccatacc aggcttggct acctcaacat agaacttcgc
    13561 atctctcgac actcgcagca cgcgggtgct gctccagaca cagcattcta tgacttgtgc
    13621 ttactagttt tggcgagatg aattaataat aattgtggtg atccgggatt ccaggcggca
    13681 cgacggtccc ggcgcccggg tgccggaccg tactccctcc cggctccggc ggcccgcccg
    13741 atcacgcctc tcttcggggg gaccggcgtg cccgctcgac cccgctcccg atgagtgccg
    13801 acgcgacgag agggagcgcg atcgttgcgt cgcagaagca ctggaccctg ggggactcgg
    13861 gagcctcttt gccccagctg atcgcttctt caaacgtgca cccggaaagc ccgccccagt
    13921 ggggggcgtc ggtggtgtac tggacggcgt aggcatgccc gccgaggtgc tgctcgtgga
    13981 tcgaggcgat gacctgggtc tgctggatga agttcttcgg gacgccgccg ccgacgtata
    14041 tgacgccggt cttctgcgac tcctcgacca tccgggtgat ctcgtcggtg tcggcgagct
    14101 ggtcgacgtc gacgtcgaca ccgcgccggc gggccataac gagaccgatc ccgatcgacg
    14161 agtcgccgag ggcggggatg aagatcggga cgtcgtactc cgcacaggtg gcgatgagcg
    14221 atcggccttc cggtctccgt gtgcaaagcc agcccccgag gcgccggatg aactctctcg
    14281 acgagccgcg gaagggcgct atctcgtcgg cgaaccgggc gatctcggca tcgacgctcc
    14341 ggaactcttc ctcatacgca aagacgtcgt atatccggtc gatcccctcc tcaaagagcg
    14401 ccgcgtcgtc ggcgtggtgg tggccgatgt agtgccggac gccgaggtgc tcgcaggtgt
    14461 cgtggaatat gtttgcaccc gtggagacga gaacatcgac gtagcggtgc ctgacgagtt
    14521 cgatgagcac gttctgcata cctgccggga tcatcgcccc cgagagcccc atgaatatcg
    14581 tgcagtccgg atcttcgacc atcctggtcc agacaccgag cgactctccg agttttcttc
    14641 cctgaaaacc ggtcctgctc attgcttgaa ggagggtcgt tacatcccta tccggtttga
    14701 ctggctgcgt tggtttcatg gtcaattctg gttatgggtt tcggccggag gatattaaat
    14761 gcctgcacgg gggcgacggt gcaccgcaaa gagcctgccg ttctccttag aacgcgggtt
    14821 ttccggaggg ctgagggaca tggccccctc caccgccgtg gcaggggtgc ttgataccgg
    14881 tatgatgggc ttcgagtgta tacgcgccta tcgccggcgt cgcgacgaac cggtctttgg
    14941 ggccggaacg gttggtgccg gcccgggaga acgaagatcg tcgtccgccc gcttcccggg
    15001 cggctgttca tccgccggtt cttcgccggg gatgccggcg atcgcccgtg catactcctg
    15061 cgctgccgga taggccggtg cgaggacggt ggagaggcca ttgccccgga ccgtgaacgg
    15121 gggttcgccg aagatcttca gggcggctcc ggcatccggg gcgctcccgg ttatctcgat
    15181 gccgaacccg gcaagcgtat cgtctccctg ccagaaactg ccggccaaag gcgtgaccgg
    15241 ctccggatcc tccgtccgcc gctccctgag tgccgccatg acctcatccc tgctttgccc
    15301 ccggagggcg aacaggagga aggggtcctc gtcaaaccgt tctgcgagga ggtaatagac
    15361 cgctgcgatg tgtttgcagg ggttctccca gtccgggcag gtgcagttcg tcgtgatatc
    15421 cttctcggag gcgggaaaga ggggaactcc tgcctcagaa aagaccttct cgatatcgtg
    15481 gggcacaaca cccatcagga gctgtgccgc aaagatcgcc tgcgcactca tcaggtcgaa
    15541 aacccggccc cactcggcgt cggtgagggc gcggaggcgg atagtgacgc cgtagggcct
    15601 cgcggcggag ccctggacct tcgcccgcac ctctccgtcc ctgacctcca taccggtcac
    15661 ctgccctttc cgggcatacc gcttcccgcg ctgcagacgt gaagcgttcc ccatctgctc
    15721 gagcgccttg atgaactgct gcgaccacca ctgctccccg atcgccccgc gcttgctccg
    15781 cgccttgagg ccgttggcca ccggccgcgg ggcggacggc ggatacgagg ggtagtaata
    15841 ccatgagccc attcgtcagt cctccaccgc atcccggcgc agggtcagga ggtcgcggag
    15901 ctgctcggtg gagagcgatc cgatccagtc ctcccctttg ccgacgatct ggtcggcaag
    15961 cgccttttta tcctcgatca gcgtgtctat ctgctcttca agcgttcccg aggcgatcat
    16021 gagccgcacc tggacgtcgc gggtctggcc gatccggaag gcccgatcgg tcgcctggtt
    16081 ctcgacggcc gggttccacc accggtccag gtggaagacg tggttggccg ccgtcaggtt
    16141 cagccccgtc cccccggcct tcagagagag gaggaagata ggcggccctc ccggctgctg
    16201 gaaacgctgg accatctcgt cgcgcttctg ccggggcgtc ccgccgtgca ggtagaggac
    16261 ggccgtgccg aaccgctcgc ggatgtaggg ctcgaggatc ttcccgaact ccgcaaactg
    16321 cgtgaagatg agggcccgtt cgcccgcatc cagcacctct tcgagcatct ccaggaggcg
    16381 cgcgatcttt ccggaccttc cctcgaaggc ggcggtgtcg tgcagaaaaa gcgccgggtg
    16441 gtcgcatatc tgctttaagc gggtgagccc cccgaggacg attcccctgc gccggatgcc
    16501 ctccgcctcg tcggcacgct cgagcatctc ctgcacgacc gcctcgtaga gcgtcgcctg
    16561 ttccgccgtg aggctgcaga agaccttcat ctccatcttc tcggggaggt cgccgatgat
    16621 cgagcggtcg gtcttcatgc ggcggagcac gaacggccgg atcagctccc ggaggtggcc
    16681 ggtctttgcc gggtcgcggt accgctcgat cggtacggca aacccggaat ggaacgattc
    16741 aaacgaaccg aggtacccgg ggttgaggaa ctccatgatc gaccagagct cggtcaggcg
    16801 gttctccacc ggcgtcccgg tcagcgcgat ccgttgcggc gctttaaaag accggaccgc
    16861 ctgcgcctgt ttcgtggagg ggttctttat gttctgggcc tcgtcgagta cgatgcagtt
    16921 ccagtccacg gatgcaagga ggtctctatc gcgcgcagca agcgggtagg tggtgatggt
    16981 gacgtcggaa ccggcggcct gccgggcaaa cgcatcgccg catgcccggg cgctgccgtg
    17041 gtggacggcg accgtgaggc ccggcgcgaa gcgggcaaac tcgcgccgcc agtttccgac
    17101 gatcgacatg gggcagatga ggagcgcggg tgtcgccggg gtgccccgct cctcttcggc
    17161 gaggaggtag gcgatcacct gcacggtctt tccaagcccc atatcgtcgg caaggcaggc
    17221 gccgcacccg ttgcgcgcaa gaaacgagag ccacgagaga ccgcgctgct ggtacggccg
    17281 gagagtgccc gagaacgccg ccggttgcgg gacggcctcg agcgttcctc cttccccgga
    17341 ccggtcaagg aacccgcgga gccatccctc cgccgcgaca tcgctcaccg ccgggccgcc
    17401 cgggtctgct cccgcggcga tgcggaggag ttcgccggcc gtcatggtgc ctccgggata
    17461 cctcctcgtg aaagccttga gcgccttctt gatctcgtct cggtcgatcg agcgccactg
    17521 gccgtgcatc cgcaccaggg ggatcttcag ggacgcgagc tcctcgaact cttccggcga
    17581 tatcgcttta tcgccgaggg cgaccgtcca gtcgtaggag acgagcgtgg caagaccgag
    17641 ccttccctcc tcctcccggg caggtttggc cgtgaggtgg agggaaggcc gggaggccgc
    17701 gctcttccac cacgcgggaa gtatgacgcc aaagccgctg tccatgagga gaggggtcca
    17761 ttcactgagg aacgcgtagg cctccgacgt atcgaggttc acccccgccg ggcgcgggga
    17821 cgtgaggctt gactggagca cggggtagat gcgggaggcg cggccgagat cggtgaggag
    17881 ccggtcctgc ggatcttcaa accgctgcgc aagagatgtg cgcacatctc cgcgcttctt
    17941 ccatatatcg ccggccggta tgacgatgcc ggggtcgtcg gcggcctgga ggtggaacgt
    18001 cagatgccat tgctcgctct cctcttcggg ctcttccagg cggaaacagg cgcggagagg
    18061 ggtgggggcg atcgtcggcg agagccagtc gccaagctgc tcgtaaaacc gtgcaacctc
    18121 ctctgcgtcg ccggcaaggt cgtcccggct gccggagagc acctccaccc actgttcgcc
    18181 gaacgggacg atctttctcg gccggccccg cggtttctgc agcagcggcc gctccctgag
    18241 ggcgccggtg acgagcgtgt ggaccgcacg gtcgagaaac gacgtcacca gagccgccgg
    18301 gtcgggcggg gacccatccg cccctgcggc ccagaaccgg caggccggcg ggagcgcccc
    18361 ggcgaggcgg gccacgcgat catggtcttc cccgcggatc agggcttccc atccgcgggc
    18421 gcccggcacg aacgactgcc gcacgacgag tccgagggca aaccgtgccg cctccgacca
    18481 gaaccggatc gtggccccgt agccgggccc ggaagggaga tccgcaaggc aggcgacggg
    18541 caccgtcccg ggcaggcgga tggcgggaac ccggtaggta acgcagtcgg cccggtccgg
    18601 cacgacggat ccgggttcgc actccggcga aggaagagga tcctgcccgg ttgccgggag
    18661 caggacggtg accgtcacgg gttcgccggc aaggtcgagc gccgcggtaa gcgcggcatg
    18721 atcggccgcg aacgggtgcg ggagaacagg cggctttttg ggcttcctgc cccgccgagg
    18781 cgtccctttc cggggcaggg ccggatcttc cccccagacg tgaaacgccc cgggagatcc
    18841 gtcggcccag aaactgtgga ggatgatcat acgatcgtgt gattatgtac tcttttttgt
    18901 accctcatca gtgcatcggt ggagcgatga ggccggcggc gcgtgggatc ggcatcccgg
    18961 gggccaacgc ccgggcgtgc ccggatccaa aaatggggtt tacccgtcct ctcctcagag
    19021 ggcctggacg agcgagtttc gggttacgac gcctgcgatc ctgccgttct gcgtgatcgg
    19081 gacggaaccg atgttcttct tgagcatcag gtcgatgacg tcggagacgt cggtatccgc
    19141 gttcaccgat atcagggggg tgctcatgat gtcgcgcacg aggaggttcc tgatccggaa
    19201 ctcctggcgg gtcccctcca cgatatcctt gagcgcgtag agcgcttttg ctacgtcggt
    19261 ctccgtgacg atgccgaggg tctggttgcc ctcttcgacg atgaaccggt tgatcccgcc
    19321 atcaagcatc ctccgccgga ggtggacggc gcgctcctcg acctggatgg tgtgcgccgg
    19381 ttccatgacg tcggggaggt tgcccggggg ccggagcacc ttcaggaggt cgcctgccgt
    19441 cacctggccg atgaggcggt gttcggcatc gaagaccacg accagtttgt agtgctggag
    19501 cagcggcacc aggatatcga tgttctggtc cggatacgcg gaggtgaagt tctcctcgac
    19561 cgtgttcgca acgtggatgg atgtggcctt cagcgcctgg gtcttcctgc tcccgagcgt
    19621 ctcggcgata gccgcccggg acgtcgtgcc gatgaccgtg ccgttgttgg tcacgatgag
    19681 ggggtcgaca ccctcatcga gcatcttttc aagcgcttcg ctgacaaagg cagattttgc
    19741 gatggtgacc ggttttgcca tcacgtcttt gacgagtatc tgaaatccgt cgctcatttt
    19801 gccacttcct tgatgatgtc atctctcttg agcacgcccc ggatatcgcc gttctccacg
    19861 accacgaggc tgttgatctg gtgctcgagc atcagcccga cggcatcctg gatggatacg
    19921 ccgggggaga cggtgacgac cgggcggctc atgatgtcct ctgcgactgc cgaaacctcg
    19981 acaacgtacc tgaaccgctt ctgtcctccc ggctcctctt tgcggagatg ggtgacgtct
    20041 tttctcggca ggttcatcct tccgtccagg tactcgtaga atgcgaggtt actctccgtt
    20101 ataatgcctg caaggcttcc attgtcgtta acgacgataa gtttatcgtt ctttcccttt
    20161 atcgtgtcga tgacgtggtc aagcgagtga taccggctga cggtgactgc atcctccatg
    20221 acctcgtcga cccgggcaaa gagaccgcgc acatgggccg accgcatgat gtccagtttg
    20281 gtgacgatcc cgaccaccct gccttcgtcg acgaccggca gccccgatat gtcgcggtcg
    20341 agcatcatcg ccgcgatatc gtggatgctg gtatcgggcg ctacggtgat cgggttctcc
    20401 gccatcagga tgctgaccgg gatccggtcg atcgggcgcc ggcgccacat cggttccgtc
    20461 tgcctgagac ggtaggcgat atccttcttg gtgaggatgc cccggagttc atcctcctcc
    20521 atcacaggca gcctcgatac gcggtacctg agcatgaggt tccgggcata ggccacgtta
    20581 tccgccggtg caacgatgta caccggggca gacatcacat ctacggcgcg catatattca
    20641 atcacccttc tgcaaatgct ttcacgagat cgtactccgt cacgaggccg acgaggtggg
    20701 agtcctctat gacggggaac gccccgacgc gttttttgat catatcgacg gcgatctcgt
    20761 ggatgttccg gtccggcgtg gtggtgtaga gttccccgga gaggagcgaa cgcaccggcg
    20821 cccccatcac ctcggcggaa tccccggtcg tcagctgttc aaaggccctg cccttcccga
    20881 ggtaactcat gatatcggtg gcggtgacga tgccgcagag gacgtcgtcc acgacgacgg
    20941 ggagccgccg gaacctgcat gtcaccatct ccctgcagac gctgccgatc ggtgtgtcgg
    21001 ggctcgtgac gcggaccgac gatcgcatga cgtcctcgac cttgcggccc gagtgctcgg
    21061 tggcgagcac cttcatcacg tcgcgctcgg tcacgatgcc cttcagggtt ccttcggcat
    21121 cggtgatggg gagcccgccg acgttgcggt tgatgatgat atcgaccgca tcggcgatgc
    21181 tcccggtaac cggcatggcg accggctgcg aggtcatgat ctcgcggagt tcctcgttga
    21241 cggcggcaag gaaattgccg ccgtgcttca cctgtacgag gttgaacttg tcgccgccgc
    21301 ccatgaagtc gatgatgtcg ctgacggtca cgatcccccg caaccggtgt gtcccgggat
    21361 cgacgagcgg cagccggcga aatccttccc gggtcatgat ctcgaccgcc tggatgatcg
    21421 tggtggtccg ttgcgccgtg acgacgttct tcgtggcgat cgccatgatc tcgccttcgt
    21481 agccggcgat ctttgttttg aagttgactg gcccgcgatc acgtttgccc ggcgatttca
    21541 ggagccgatc ggccggtttc ttctctgcgt tattcgagtt cggttgcatg gtatcactcc
    21601 tctggcgata atagtattca ggggtggcgc ccctcacggg gcgaggcaac ttgagacgtc
    21661 gtggcggtcg atgacaccga cgatcctctt cttctcgtcg atgaccggaa ggatgctgac
    21721 gtcgtggtcg accatcagcc gcgccgccgt cgcgaccgga tcgtcaggcg tcaccgtgat
    21781 gacgggggtc gtcatcaccc ggtccacggt ggtgtcggcc tggttcttca ccgacatgcg
    21841 gacgcttccc gcgcgcagga gatcccggcg ggatatgatg ccgatgggtt ccttcttctg
    21901 caccacggga aatgcgatga agccgcttgc gatgatcagg ttatagatgc ggtggatggg
    21961 gtcctcgggt gcgcaggtga cgggttcggg cgacatggcg tctttgaccc tgccgcagat
    22021 atcgcggcgg gagatcagga tggggaagag ttccgagaag agcacgccgc ccaggaagac
    22081 gccgtcttca tcgacgactg cggcgctgtt cgtgcgggcg ttcatgatgg cgcggccgac
    22141 cccggtgaga ggcgtatccg gggcgacccg ggcggcttcc ctgacaaacc cttcgatggt
    22201 gacgttggac ttcgtatcca cgacgcgcag gacgtcggat atgtcgaggt agcctgccag
    22261 ccggttcttc tcgtccagga cgtagagttc gcgaaagacg tcgtcccgaa ggatgctccg
    22321 tgctttcgtg atgtagtcgc cattgcacag ggtgggtata tccaccatca ggttctcggc
    22381 cactttcata gcaactgttc ctcctcccga caggcgtggc agagcataag gttgtcgacc
    22441 cgcgagaggt catcggacat atccccgcac cggtcgcaga cgcccatggt gtagacttcc
    22501 tcgcgattga tctcgatgag atccgccagc agttcgttca cctcggccgc gacggtgagg
    22561 atatccctga ccgtgacgat cccgatgacc ctctggttct cgacgacggg gagccggcgg
    22621 acgcggtgct tcaccatcat ggccacggcg tccccgacca tcctatcggc gccgatcgtg
    22681 atcagcgggg tgctcatgat ccggtcgacg tgaacgttgc cgggcttcat atcctttgca
    22741 acgactttac agttgatatc ctcctccgtg acgatcccga taggcaggct gttttgcagt
    22801 acgatacaac tgccgacctc gtcgcggcac atggcctgtg ccgcacgggc gaccgtctcc
    22861 ccaacatcga tggtcgttgg atggctccgc atgacctccc tgattggtac ccgtgtctca
    22921 aagtggatga cgtcgctatt gttcattatg ggattctcct aatctggtcg cggtgagagc
    22981 cgattctcca ggtgcaagtg tatctaggtt tttgcagtat aaaagaattc tctcggcggg
    23041 aacgattacc gtttatcccc gatttgaggg gattacgccg gatggaacat ataattatat
    23101 ttattcgggg gtgtcatgtt tacccaggtg taggtgaatc ggcctatcgg agggtgccaa
    23161 atgacgttct tagttcgaac aaaacagaag atctcgcggt tcctcaagac gctctttaaa
    23221 aggcggacct gccgcatcgg gatctacggg cctcccaatg caggcaagac gacgcttgca
    23281 aacaggattg tgcgggattg gacaggagat gcagtcgggc cggtcagcga ggtgcctcac
    23341 gagacccgcc gtgtgcggcg caaagaggat atcaccatat cgagccagaa cggcaactcg
    23401 gtcacgatcg atatcgtcga tacgccgggc gtgacgacca agatcgacta taacgagttc
    23461 gtcgagtacg gtatcgagaa ggaggaggcg gtcaagcggg ccagggaggc aacggaaggc
    23521 gtggccgagg cgatgcactg gctccgcgag gatatcgacg gcgtcatcta catgctcgat
    23581 gccacgcagg atccgttcca gcaggtgaac atcatgctcg tcggtatcat cgagagccgc
    23641 aagctcccgg tcctgatcgt cgcaaacaag aacgatcttc ccgatgcttc ccccgcgcgg
    23701 atcaggagtg cgtttcccca gcaccccgtg atatcgatat cgagccttga ggggaacaac
    23761 gtgaacgagc tgtacgagaa gatgacatcg tactttgggt gatggagatg atacagggtg
    23821 tacagataga tcttatctcg gcggagcgcc ttgaccggct gacgacgatg gagaagattc
    23881 gcctgatcct tgacgatgtc atggagggga acatcgtcgt tctggagaag ggccttgcgc
    23941 cggacgagca gagcaagctc atcgagatca cgatgcggga gattgcgccg gacgggttct
    24001 cggggatcga gatggagacc tatcccgtca gggatgcacc gaccggattt cttgccaaac
    24061 ttctcggagg gaagcgaacg gaggcccggc tgaccgtgat cgggcccgca aaccagctca
    24121 agacgctcag gaaggagaag gatctcatca gcgcatgggt atcctcctcg aggtaggtgg
    24181 ggtgaggggg tatgcctcac aagtgcacgc aatgcggcag ggagtttgaa gacggttcga
    24241 cgaagatact gaagggatgc ccaagctgcg gcgggaagaa gtttctctat atccgtgagg
    24301 ccgagcggca cgacgacgtg ctgaaagaga agacgatcga ggagatagcc cgggagacgg
    24361 gcgaagatgt gcttgaggtc cgcgaggata agcgaagggc ggagatcgag gtctttgagc
    24421 ggatcgagag tatccgcata ctcggtccgg gttcgtacga gttgaacctc gagagactgg
    24481 cccggtccga tgaggtcgtc gtcggcctgg agaaggaggg gcgatacgcg gtggatatca
    24541 tctccatggc gaagaagaag aaataatcgg catttgcgag ccttcgcacg aaaacagggc
    24601 ggttttcgcg cgatgatgcc cccttcccaa aactattata tatctcaagg accctttttt
    24661 gatacctggt ataacccggc accctctggc gcatacccgg gcgatggttt cctgcatggg
    24721 agtacggcac tctcatgaac cctttaccgt acctgactgc cgtcattcat gctctcatga
    24781 ggtatatccc cggttgcacc gcacagcggg ggatgccgac atgaaggaag agtatgaatc
    24841 cgacatatat cacatcactg gataaggtcg ccggcatcgg tgcacaggag cgtgccgctc
    24901 ttgcgccggt gctggagaac tttgcgttcc gctcgaatga ctactacctc tcgctgatcg
    24961 actgggagga cacaaaggat cccatccggc gcctgatcat accctcgccg gacgagcttg
    25021 agccctgggg gcgcctcgac ccgtcgtcgg agcaccagta cacccgggcg ccggggctgc
    25081 agcataagta ccgggagacg gctctgctcc tcgtgagcga cctctgcggc gggttctgcc
    25141 gctactgttt ccgcaagcgc ctcttcatcg aagatgcgcg cgaggtgaac aaagacgtct
    25201 cgggggggct tgcctacatc cgcgaccacc ccgagatcac gaacgtcctc ctgacggggg
    25261 gcgaccctct ggtcctcggg accgaccggc tgcgcgatat actcaaccac ctccgggcga
    25321 tccggcacgt ccggatcata cggataggga cgaagatgct ggcctacgat ccgtaccgga
    25381 tcatcaacga tccggccctc gtggatgccg tccgggggtg ctacgacggg gagaacctgg
    25441 tctacatcat ggcccagttc aaccacccgc gcgagctgac cgatgcggcg tgccgggcga
    25501 tagcgctcct gcaggatgcg ggagcggtca tcatgaacca gacgcccctg atccgcggta
    25561 tcaacgacga cccggcggtg ctgtcgaagc ttctcgggcg gctctcgttc gtcggggtca
    25621 acccctacta cgtcttccag tgccggccga cgaccgggaa ccggacgttt gcggtgccgg
    25681 tggaggagtc ttaccgtata ttcgagcagg cgcgggcgac ctgttcgggg ctctcgaagc
    25741 aggcgcggtt tgtcatgtcc catgcgacgg gaaagatcga ggtggtgggg aagaccggag
    25801 ggttcacgta cttcaagtac aaccaggcgg cggacccgga cgatatcggg cggtttatgg
    25861 tctataagag caacccgcag gcctactggt tcgacgacta cacggacctc gtggatgagg
    25921 ggaggctcgg ggatcctgag gaggcggtgg gcgatgcccg ggatctcgcg ctgtcgccgg
    25981 gcgcggggta cacgctgggg tgaggggggt cccggggatg gtttctcgcc ggcagggtgc
    26041 cggtctcccg gcatctcctg cgaagtcggg tgcgccggct ttatggagga gaggtctgag
    26101 gtttatgttc cggcaaccgg cggtatcgga agtccgcgcc ggagcaagag gatcagccat
    26161 tcgtcgatga cgccggcaag gtttctcctg cactcttcga gtgtcctccc ggttgcccat
    26221 acgccgggga gttccggaac ctctccgtag tatggttcgg catcctcgat gatctcgtag
    26281 cgggcgtggt cgagtgccgc gttgatgtac tcaaggacca tactacgcat ctgtagggtt
    26341 ccaactatag ggttttcgtt tgccggttgg agccggaggc aactttgcct catgatatgg
    26401 atcgttgcag cctcgccgac ctgtct
//
LOCUS       DAOSII010000012        26222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_2068_length_26222_cov_51.497153, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000012 DAOSII010000000
VERSION     DAOSII010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 26222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26222
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2068_length_26222_cov_51.497153"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PLV90_01850"
     CDS             complement(<1..592)
                     /locus_tag="PLV90_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95887.1"
                     /translation="MRPLLAVIVGITIIYACMHFFVLPEAPDVTRIENITYLESPAYN
                     PAILDLWKIVVRETGVENESAVLRQLEVDLAKDGAIQVIWFYFYGVESGKQHVYEVYV
                     GPGGTVSVKSQEFNHTVQGVHPLALLEEVDAIALEDIPFREQGMTILVSPNAYGDHYN
                     ETRGRLYEVSNGAFLPVKEATFGPDASWYTITITPHR"
     gene            776..1195
                     /locus_tag="PLV90_01855"
     CDS             776..1195
                     /locus_tag="PLV90_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95888.1"
                     /translation="MATESMVTGETQERRVSPGMKAERVFTVARKEFTDQVTGWRFLV
                     ILALFLAIALVGTYSGVGRYERDLDRYAQQLATMDNQNDGPAGMMPAKPPVEGVYSSM
                     FRTLVSYGGLLALALTAYPVVFFAVAYTRLMRIDIRG"
     gene            1644..2252
                     /locus_tag="PLV90_01860"
     CDS             1644..2252
                     /locus_tag="PLV90_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95889.1"
                     /translation="MTQEIRSLNQADTQIPAANRHIRQDRDCHSSAGAYLPFMIGYYR
                     HGLSDCGGGLGPWNSADCLPNVMIRYARTRKCLAHLQTLAGCYWMEIDGCPEHCYIEG
                     TFNLDFYMVSLINNSREFGHVICAEFLGSDMQTFSSWKFYQFDNLDIRPGNWQMPYGT
                     ETEDTKVKIYEITGIFNCGLPDHDLVPLVTFLIKENGGVTKG"
     gene            2267..3043
                     /locus_tag="PLV90_01865"
     CDS             2267..3043
                     /locus_tag="PLV90_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95890.1"
                     /translation="MTKATLRIIIALAIVCCTGAMIVTVHLPDSYPHPEVGNVTPEGS
                     PLGSDLPGGISSNATPNEVPEEMQEVRNMTPERSSQGLYVHPFVRQALEEANMTAEVL
                     PEELTEEIEQLQRSYEDRKFGISGWEFDSENKLVIIYAYSILDEKKVKAIQGRQAGGW
                     TFKVIHNLDYENERKQAAAELMQFQNDHPELEISGFIITSPAHVEMWVRNRTPENEAL
                     NGTVMYGRTIELIGGAPREELMMREELKWNEPASREPEAG"
     gene            3408..4427
                     /locus_tag="PLV90_01870"
     CDS             3408..4427
                     /locus_tag="PLV90_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOF95891.1"
                     /translation="MATESMVSGKVQMDRVSRGMKTERVFIVARKEFTDQVTGWRFLV
                     ILALFLAIALVGTYSGVVSYERELDRYSEQLAAMDDRFDGPERMMPTKPPVADIYSSM
                     FLTLISCGGLLAIAVGFDLVSREKESRSLKSILSHPVYRDEIINGKALGGIALLALVV
                     GGVLAISTALLLVFSIVPAPGDLWMVLTYAGVTLLFFVTFFSIALAISTLCRESGSAL
                     LLSMIVFIFLVLLAPYATTHIGMAFMMEEPDPAAYGGDISSEEFQAAITAYYGQFNLI
                     QSITNLVSPQMATNALIQGISNSSGATLEDMLGRIWSSIAALTIYPVIFFAIGYTKFM
                     RMDIR"
     gene            4602..5162
                     /locus_tag="PLV90_01875"
     CDS             4602..5162
                     /locus_tag="PLV90_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95892.1"
                     /translation="MSGNIIHPLPRSRFSPALPPLVAAVTAVIWAGTARIVSGTPGPA
                     PPFVLPVFILVIVALVVAGAVTPLPLIEGAVPVKRQRQAMLVAIAASVPFVGALVINP
                     ERWPGGPAPLIAESVPIFGWFFDGIMSVLPLAADTPTYKLFFSAFAVFGFYLECVLVA
                     AILYVVLRVLAALDPGPRGESRPEEE"
     gene            complement(5167..5832)
                     /locus_tag="PLV90_01880"
     CDS             complement(5167..5832)
                     /locus_tag="PLV90_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOF95893.1"
                     /translation="MATFDPSVLGVFVFGLVAGICPCNSVLCLGLIGYLTSGKTDLTL
                     AGILRLTIAFSFGTILVLLPLGVIAGYLGEYLLFLDETIAWAIGGVLMILMGLQLLHA
                     YKPPIRRIFNFFRAPISYSVTGAFLLGLSFGAITVGRGAPMLLIVLTYIALYQTAIEG
                     LFTIFVYAVGLSIPLIVLSSVGGAVGKKVKEKARMSGEVFDRIIGIAIVLIGVYFLTL
                     AFF"
     assembly_gap    6149..6248
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6323..7213
                     /locus_tag="PLV90_01885"
                     /pseudo
     CDS             6323..7213
                     /locus_tag="PLV90_01885"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
     gene            complement(7937..9040)
                     /locus_tag="PLV90_01890"
     CDS             complement(7937..9040)
                     /locus_tag="PLV90_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-NAD kinase family protein"
                     /protein_id="HOF95894.1"
                     /translation="MRKTVGFLVNPVAGMGGAVGLAGTDGKVSEALLRGAVPRARDRA
                     VQALSLLRDDDITWYTCAAPMGEDALAGAGIDRFTVVYRPSGPTGAADTKAACRAFLD
                     AGLDLIVFCGGDGTARDVFDAVGRSVPVLGIPAGVKMYSAVFAVNPAAAADLIRRAGG
                     VACRDSEVMDVDEEAYRSGRLSARLYGYLRVPYIPERTQGGKQVFEQQDEERAKDDIA
                     AFINEVMLPETLYIIGAGSTTARIMERLGLTPTLLGVDVVRDGRVVARNTDERTLLAL
                     LDRYPRAKIVVSPIGAQGFVLGRGNQQISPAVLRKTGLSNLVVVATPGKLAATPLLYV
                     DSGDPALDRDIGDSLLVVSGYRVAQRKRVLHPE"
     gene            complement(9037..9873)
                     /gene="trpA"
                     /locus_tag="PLV90_01895"
     CDS             complement(9037..9873)
                     /gene="trpA"
                     /locus_tag="PLV90_01895"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOF95895.1"
                     /translation="MSRIEALFARKNPVFVGFTVAGDPDRETSFGVAKTMVDAGVDLL
                     EIAVPYSDPVADGPVIERAHVRALRAGTTPDDVFALVRRVREYAPPLPLVLFTYYNIV
                     YRRGVDRFFAEAAAAGADGILIVDLPTEESGEVAPSALRHGIDRIVLVAPTTSPERRQ
                     KILGGASGFVYLISLEGVTGERDHLPPNLAGLVGAVREKTDLPLAVGFGVSRPEHVAA
                     VAAAGADGVIVGSALVRVIEEHPGDEAGMHEVLRAVIRALKSGFAPDPDPDMPRRERV
                     EG"
     gene            complement(9870..11042)
                     /gene="trpB"
                     /locus_tag="PLV90_01900"
     CDS             complement(9870..11042)
                     /gene="trpB"
                     /locus_tag="PLV90_01900"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOF95896.1"
                     /translation="MRAGRYGVYGGQYVPETLMSALIELEETYARVREDPEFSRRLSW
                     YLHEYAGRETPLTFCGNLSRDLGCRVYLKREDLLHGGAHKLNNTLGQALMAKFMGKRR
                     LIAETGAGQHGVATAIAGAVLDLPVEVYMGEVDTRRQALNVFRMELLGARVVPVASGT
                     RTLKDAINAAMRDWVANFGDTHYLLGSCVGPHPFPRIVRDFQSVIGEEARRQILEREG
                     RLPDTVVACVGGGSNAIGIFYPFVNDDVRLVGVEAGGEGLISGRHGASLSAGSVGVFQ
                     GALSYLLQDGDGQALETHSIAAGLDHPSVGPEHAMLKDAGRVRYEAVTDAEALHAFRY
                     LSRIEGIIPALESAHAVAYALRAADNLDEDDILVVNLSGRGDKDVADVANVHGGAA"
     gene            complement(11039..11611)
                     /locus_tag="PLV90_01905"
     CDS             complement(11039..11611)
                     /locus_tag="PLV90_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOF95897.1"
                     /translation="MKICGITTVGDARAAAAAGADAIGVVLASPSPRSVTPELAREIF
                     AAVPPFVTTVAVTSTGREEDLAAILSSRPDAVQVAGDLALPRGAGVRVIRMLAPGDAL
                     RDDCDAVVVDGSRGTGRAFDPEYARRCVASSPVPVILAGGLSPGNVAAAIRAVRPYAV
                     DVASGVEAAPGIKDERLMRAFVMACRTIEP"
     gene            complement(11608..12366)
                     /locus_tag="PLV90_01910"
     CDS             complement(11608..12366)
                     /locus_tag="PLV90_01910"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOF95898.1"
                     /translation="MILDDIVRATRERLESLDTLPEIDPGAPPHRSLEAAIRACRERR
                     AIIAEVKYASPSRGRIHDGCAPETIAREFAAAGAVGLSVLTEPAYFFGSIENLVRVRR
                     AVTVPILRKDFIVDERQLAETRALGADAVLLIARVLGDRLPAFVDGAHALGLEALVEV
                     HDRSEMERALATDARLIGINNRDLETMRIDLSTTFRLAEAARDAGRIVVSESGITWPY
                     DVRSLGRHCDAFLIGSALMSAKDRCKRLEGFVFA"
     gene            complement(12363..13379)
                     /gene="trpD"
                     /locus_tag="PLV90_01915"
     CDS             complement(12363..13379)
                     /gene="trpD"
                     /locus_tag="PLV90_01915"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOF95899.1"
                     /translation="MIREAIVRISTGTDLTSAEAGRVMEEIMRGAATPAQVGGFLTAL
                     RMKGESETEIAAFARAMRAAAVPVVLPAPEMRVDTCGTGGDGAGTFNISTAAAFVAAG
                     AGVPVVKHGNRGVSSRCGSADVLEALGVSVAIPPDKVGEVLAVAGIVFLFAPAYHPAM
                     QYARSARQEIGIRTVFNLLGPLTNPAGAGAHLLGVYDPRLTLPVARVLGDLGVERAMV
                     VHGAGLDEIATAGPTTVAELRDGEVETYTLDCMEFGIPRSPVAALRGGGPEENARLLL
                     SVLAGEDGPARDIVALNAGAAIYLGGKAGDIAAGIARAEASIDSGAALDRLRRLVEAT
                     GGPV"
     gene            complement(13376..13957)
                     /locus_tag="PLV90_01920"
     CDS             complement(13376..13957)
                     /locus_tag="PLV90_01920"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOF95900.1"
                     /translation="MRVLVIDSYDSFTFNLCQQIGMLGAEPVVVKSDTLFERIRPVLF
                     DRVVLSPGPGHPRDSALYREVLGTISRTVPTLGVCLGHQAIGLAFGARIARADRLMHG
                     KRSMIRHDGAGIYTGVASPLVATRYHSLVIDPATVPDCLEVTARSDDDGAIMGVRHRE
                     FPIEGVQFHPESILTPDGNRLMKNFLSGMGGAA"
     gene            complement(13957..15360)
                     /locus_tag="PLV90_01925"
     CDS             complement(13957..15360)
                     /locus_tag="PLV90_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOF95901.1"
                     /translation="MPLPAPSPADLYASLRDGPGFLLESLEGSGKTARYSFICTAPAA
                     TVVVASDGALTVSGDPRIRAIAGEVEAADPIGAARSFMARFRVAPSPLRGFSGGLAGY
                     FSYDLISSIYPVSRVETAEGSEEPAARFMLAQNCLALDHRRERLAVVGNLLVTDGADA
                     EEEYHRAREAIAGALARIRDLTPACTGYPPSSGVVTSSCTPEEFSGAVLRIKEHIAAG
                     DVFQAVLSRRLACRIAGDPFEVYRRLRVKNPSPYMYYLDFGDMAVAGSSPEMLVRVED
                     GRVTTVPIAGTRPRGRTPAEDDRLAAELLADEKERAEHIMLVDLARNDVGAVSRYGSV
                     SVEGFMTIERFSHVQHIASTVTGTLREGLDRFDALRSCFPAGTVSGAPKVRAMQIIGE
                     TEKLRRGIYAGAVGYLGFSGTMDLAIAIRTIVVEDGAASVQVGAGIVADSDPGREWAE
                     TENKGRAMLAALGAEAE"
     gene            complement(15476..16834)
                     /locus_tag="PLV90_01930"
     CDS             complement(15476..16834)
                     /locus_tag="PLV90_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOF95902.1"
                     /translation="MQTKILLDEGEMPKRWYNIQADLPTPMDPPLHPATGKPAVPDDL
                     RHIFPMELIRQEMTTERYIDIPGEVRDILTLWRPSPLYRARRLEAALKTPAKIYFKWE
                     GVSPPGSHKPNTAIPQAYYNREEGVERLATETGAGQWGSSLAFAAGLFDMECTVYMVR
                     SSYEQKPYRKSMMQVYGAECIPSPSDKTRSGRAVLAHSPDTPGSLGIAISEAVEDAAN
                     HENTNYALGSVLNHVCLHQTIIGQEAREQLAMVEAYPDVVIGCVGGGSNFAGLSFPFA
                     GDKITGKHPETDIVAVEPFACPTLTKGLYAYDFGDVAGLTPMLRMFTLGHDFVPPAIH
                     AGGLRYHGMSPLVSRLARDGVVRPVAYHQNEVFDAAVMFARTEGIIVAPEAAHAVKAA
                     IDEAVRCRTTGEAKTILFNNSGHGNFDFSSYEAYFGGRLVDYEYPVELIKESLARLPA
                     TG"
     gene            16972..17553
                     /locus_tag="PLV90_01935"
     CDS             16972..17553
                     /locus_tag="PLV90_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOF95903.1"
                     /translation="MYLLAHAMVGILIGIVLAAIVGDRRLIALGALGAVLPDLIDKPL
                     GHIVLAGTVNYGRIYFHGLTVLSLALLAGFLIYHYRGQVGLLVVAAGMASHQVLDGMW
                     RQPVEWFWPFLGPLPQHDYPGDYFWSSILRELAQPSEWLFFLLIAGLFTVLYRREIGA
                     LLTGLADPGTWRMLVLVLGLAAVAALVVGARFL"
     gene            complement(17572..18423)
                     /locus_tag="PLV90_01940"
     CDS             complement(17572..18423)
                     /locus_tag="PLV90_01940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOF95904.1"
                     /translation="MKDLPMPDWNEVWRRRLALNRSTPAFREGVELWSDRERARRYAT
                     RLASDHARRVAETLSDLDVAPGDRVLDIGSGPGTLALPLARAGALVTAVEPAEGMLAE
                     LRAAAEQEGITTITTVRGLWEEIDPACDIAPPYDRVVASFSLTMPDIRSAAAAMNAAA
                     SESGSVHLYWFADEPLWERIYLALWEDLHGAPYHPRPKADCLFNVLYGMGIYADVRMR
                     PMGDTVAFTTVEDAVDHFTPRMGVTTLRQREILRDYFREHLIRQDGGLALPGRSIYAA
                     IRWQKQG"
     gene            18541..>18895
                     /locus_tag="PLV90_01945"
     CDS             18541..>18895
                     /locus_tag="PLV90_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HOF95905.1"
                     /translation="MNSVTGESMLQITVDQLARSLCASAVLGAPVETAERVIIPVAEF
                     GFGFGAGECKGGTKEGNQGGGTGTGGGGGISAIALVIVTKGVSGPEGIQVISLKKKSE
                     IAEVITTLGEAVGPHV"
     assembly_gap    18896..18995
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18996..19542
                     /locus_tag="PLV90_01950"
     CDS             <18996..19542
                     /locus_tag="PLV90_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HOF95906.1"
                     /translation="GTATVAWGIVGARVRADEDVQVFEVLLAGRRVMVRDIREMAAAK
                     PEEEKKVEEPKPALPVGEYVDAARDLWPHIQRIVAAIYRSLYLETLRGEVTLGLESPA
                     STGVVYGYCTAARYALWPAEAIDLVLTPDFDHEVFEGTFTLRVQIRRPLLIVIPVVQA
                     LLQKPVRQRLRQVSGRGAPGA"
     gene            19535..19789
                     /locus_tag="PLV90_01955"
     CDS             19535..19789
                     /locus_tag="PLV90_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95907.1"
                     /translation="MREETELVIGGARRAGDRYIIPVIRTLVLHAGRTAVVSLTPVAL
                     IIAEGECEYLIRLPGAPRSIESALDTLRGDIDRQKRECPG"
     gene            complement(19793..20938)
                     /locus_tag="PLV90_01960"
     CDS             complement(19793..20938)
                     /locus_tag="PLV90_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF95908.1"
                     /translation="MTFFDLARRNVSRHWLRSILAVTGIAIGVVAIATLGIMGNSINL
                     MFNDMVSDVGDTITVSPATRSGTTELTKRQVDDITRAVGANRAIPFATTFDSVTVGET
                     KSGAMIYAIPAKDIPFLLDMEAGAYPKDTGSGCVIGKTLAENSKDTGLKLGARATIGN
                     ETLRVVGVLEERGMGFDINPDSAIVVPYSWYSNHYDKDTYDQVIVKVRDVNDLDAVKE
                     AIEQKMNRRENVVTVMDTRGILKEVFAAVDSITVFLMGIGAVSLIVAGVSILNVMLMS
                     VTERIKEIGVLRSIGTRRSEVMRMFIYEALILGLVGAAIGGVLSFGTGYMMTAVFSGN
                     PDYFFNPTSLLYIVFGMAFGIGMSAVSGIYPAWKAAHLNPIQALRHE"
     gene            complement(20935..21603)
                     /locus_tag="PLV90_01965"
     CDS             complement(20935..21603)
                     /locus_tag="PLV90_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF95909.1"
                     /translation="MPVISFDAVTKVYPLPAGDVVALAGIDLAIEEGEFVLIMGPSGS
                     GKSTLLNVMGSLDVPTSGEVTIAGKRISRMDDDELTLMRRDHIGFIFQQFNLIPLLSV
                     VENVEYPLILKGRRDVSRERAEEVLAAVGITESHFTHKPGELSGGQQQRVAIARALIN
                     DPDFLLCDEPTGNLDSKTGTAIMELLGRMNREEGKTVIMVTHDPRMTEYADRTIRLVD
                     GRLA"
     gene            complement(21609..22745)
                     /locus_tag="PLV90_01970"
     CDS             complement(21609..22745)
                     /locus_tag="PLV90_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95910.1"
                     /translation="MRPLYFIIISLVCALAAFTGPASAGPADITVTSSSIDPPILMKG
                     DTGTLTIAIQNNGAEPVAIRSARLFGSGVVPLSDSYPAVGELGTKNTKTFTFTVRADG
                     GEGTFYPVFVLDFVNAGSLRYPIPVQVENTPLSISVIKKPDAFSEGRTADVTIRVGNP
                     RPNAASGVQVIPQGTGLKAIPSSAFIGALAPDASGTAVFNLTPAAETTAAFQVVWRNG
                     INTHTADLILPITFDEDKRQADPVITNVEVRQEAKGYRIVGDVMNAGLESARSVIISP
                     GEPATPTDPYRIYIVGTLDPDDASPFEVTLQADADVTEIPIVIEYRDADGNRYTATTM
                     VDVGGTTTALSGGEDGELPLAGIVVVVLLALGIVGAIYYSWRRK"
     gene            complement(22748..23404)
                     /locus_tag="PLV90_01975"
     CDS             complement(22748..23404)
                     /locus_tag="PLV90_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HOF95911.1"
                     /translation="MEIGFLRVLFDPGRFFESRMRDEPSLKVPALIALVIGVIGAVSA
                     ALAANMFVGILPAEAQAIGVLMVGFAAVVAVVGGFLTWFIYGIVFYIISMAFKGEGSL
                     ARTLEVTGYGFLPQIFGGIIGALLSYQIIANLTLPIARSPEEIAAVTENLAHVIATDP
                     LAQIAGVVTILFLAWSANIWVFGMKHARNLSTRNAALTVGIPVGLYIIYLLITLAGWL
                     "
     gene            complement(23744..24415)
                     /gene="radC"
                     /locus_tag="PLV90_01980"
     CDS             complement(23744..24415)
                     /gene="radC"
                     /locus_tag="PLV90_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOF95912.1"
                     /translation="MKKMRDIPDHDRPREKLAARGPQSLTNAELIALLLGRGVKGRDV
                     SQIAGDVKQYLDNAKGNPSYDALLVIEGIGAAKACEIMACFELGRRYLADDEGVAGSR
                     ITKPDEVLPLVSEWRDKKQEYFFCITLNGAGEVIRRRVVTIGILNQSLVHPREVFAEA
                     ITDRAASVILVHNHPSGTLDPSTQDISITRQLIEAGSILGIRVLDHIIVTKKGYVSLK
                     ELGHM"
     gene            24613..>26222
                     /locus_tag="PLV90_01985"
     CDS             24613..>26222
                     /locus_tag="PLV90_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOF95913.1"
                     /translation="MELPWRSTEVWCRKGRAYCEEGQYDRAVECYDRAIRLNAGAADA
                     WYHKGLALTAAGRHQEAAGCLDQATRIDPASARSWCARGQVLYNLRQYQEAAECCSQA
                     VKLAPDSADAWLTRGHAFRSMGRTPDAIRNYDQAIAIDPGRVETWLARGTALATERQY
                     DAAIDCYDQVIALDPGNANAWYARGTIQAILSRHNDALASCDRAVAIDSNHADAWYVR
                     GCTLAVLEQYAEAINSYDRVLAVRPGDADAWYNRGRALHRLERHAEANECYDRALRIK
                     PDYAGIWYHKGTALRKLKRYEPALASFDRALKENAADPGIWYQEGLILFSLKRFEKAI
                     ECFDQVLRLRTDHPDACYYRGESYYALGDYGAAVTCYRTVVRMDPENAVAWNNYGNAL
                     YRLERYEEALACYERALGVNPENQGAWSNKANVLSILTHYDEALACYDRELRTNPENA
                     DAWYNKGLALFILGCYGEAAACYARALAIEPARAEVWCARGNTLVILERYEEALDCYD
                     RVLAANPDDPGALKGKAMALIYIDRPAEA"
BASE COUNT         4802 a         8272 c         8358 g         4590 t
ORIGIN      
        1 cccggtgggg agtgatcgtg atggtgtacc aggatgcgtc cgggccgaac gttgcctctt
       61 tcaccggcag gaacgctccg ttcgagacct cgtagagcct cccccgggtc tcgttgtagt
      121 ggtccccgta cgcgttcggg ctcacaagaa tggtcatacc ctgctcccgg aaggggatgt
      181 cctcaagagc gatagcgtcg acctcttcga ggagggcgag agggtggacc ccctgcacag
      241 tatgattgaa ctcctgcgat ttgacggaga cggttccacc cggtcccacg tagacctcgt
      301 agacgtgctg cttgccgctc tccaccccat agaagtagaa ccatattacc tggatcgctc
      361 catctttggc gagatcgacc tccagctgtc gcagcaccgc cgactcgttc tcgacaccgg
      421 tctccctaac gacgatcttc cagaggtcca ggatggccgg gttgtacgcc ggggattcga
      481 gataagtgat gttctcgata cgggtaacgt ccggggcctc cggcaggaca aagaagtgca
      541 tgcaggcata gatgatcgtt atccccacga taacggcaag gagcggtctc atgatttata
      601 gttccggtcg ggattccaga gatacgaggg gtagctgcag agcatgatcg tcaaagggtt
      661 tcacccggtc gatccgcgac ccgacgaccg gtaccatgct ggagatctca acgttctgct
      721 atataccttt tgtgacaggg atcatcgaac ctcctggatt tgcgaggata atgggatggc
      781 aacggaatcg atggtaacgg gggagacgca ggaacgccgg gtttccccgg gaatgaaggc
      841 ggaacgggtt ttcaccgtag cccggaaaga gttcaccgac caggtcacgg gctggcggtt
      901 cctggtgatc ctcgccctct tcctcgccat cgccctggtg ggaacctaca gcggggtcgg
      961 gaggtacgag agagacctgg ataggtatgc ccagcaactg gcgacgatgg ataatcagaa
     1021 tgacggaccg gccgggatga tgcccgcaaa accgccggtc gagggcgttt actcctcgat
     1081 gttccgaaca ctggtctcct acgggggcct ccttgcgctt gcgctgaccg cctatcccgt
     1141 cgtcttcttc gccgtcgcat acacgcggct tatgcggata gatatccgag gatagacagg
     1201 cgcgcacttt cgctccccga gaggtgctgc atcggggcgg gccgataatt ccgccgggct
     1261 tcgatgtccg ggaaaggtat gcagggctct catcggagta tgcactcata cccgcgatcc
     1321 ggggatcgag gtatatttcc actggatatc ggcagtactg ccgggagatg gaacaacgag
     1381 ggcgtatcag gagaatgcga cggccagggt acaaccgcga cagttcgatg tttgtcacag
     1441 aaaaggtata tgggggaacg ttgacgcctt gatcggtgac accgtgtacg gtatcccgcg
     1501 ggggccggga accccgattg ccgtcgtatc ccgcccccca aaagcgtgcc cggagggcca
     1561 atagggctgt aggctgccat cagggatagt cgttgtggtc tagcgttcct ccgggcatcg
     1621 tttcgaagac tggatgaaca cgcatgacac aggaaataag atcactcaat caggctgata
     1681 ctcaaattcc tgccgccaac cggcacatcc gccaagatag ggattgccac tcgtcggcag
     1741 gcgcgtatct gcccttcatg atcgggtatt accgccacgg gctttcggac tgcgggggag
     1801 gtttgggccc gtggaacagt gccgattgcc tgccgaacgt gatgatacgg tatgcccgga
     1861 cccggaaatg cctcgcacat ctccagacac tggcgggctg ctactggatg gagatagacg
     1921 gctgccccga gcattgctac atcgagggga ccttcaacct ggatttctac atggtgtcgc
     1981 tcataaacaa ctcccgggag tttggtcatg tgatctgtgc cgagttctta ggcagcgata
     2041 tgcagacgtt ctccagctgg aagttttacc agtttgacaa cctggatatc aggcccggga
     2101 actggcagat gccctacggg acggaaacag aggatacaaa ggtgaagatc tacgagataa
     2161 cgggcatctt taattgcggt ctcccagacc acgatttggt gcctctggtg acgtttctga
     2221 tcaaggaaaa tgggggtgta accaagggtt gagagaggag gaataaatga caaaagcgac
     2281 cctacggatc atcatcgcac ttgcaatcgt ctgttgcacg ggagcgatga tcgtgacagt
     2341 gcatcttccg gacagttatc cccatccgga ggtcgggaac gtgacgccgg agggatcgcc
     2401 actagggtcg gacttacccg gcggtataag ctcgaatgca acgccaaatg aggtacctga
     2461 agaaatgcag gaggttagga acatgacacc ggaaagatca tcgcagggac tatatgtgca
     2521 tccgttcgtg aggcaagcgc tagaagaggc aaatatgacg gcggaggtgt tgccggaaga
     2581 gctgacagag gagattgaac aactgcaacg aagttatgaa gacagaaagt ttggtataag
     2641 cggatgggag tttgactcag agaacaaact ggtcatcatc tatgcgtact ccatactgga
     2701 cgaaaagaag gtgaaggcca ttcagggcag gcaggctggc ggctggacat tcaaggtcat
     2761 ccataatctc gattatgaga atgagcgtaa gcaggctgcg gctgaactga tgcaatttca
     2821 gaatgatcat cctgaattgg agatatcggg ttttattata acatcacccg cccatgtgga
     2881 aatgtgggtg cgcaaccgta cacccgaaaa cgaggcattg aacggcaccg tgatgtacgg
     2941 taggacgatt gagttaattg gaggcgcacc aagggaagaa ctaatgatgc gggaggaact
     3001 gaaatggaac gaacctgcat cgcgggaacc ggaggcgggt tgaggcgcag agcgaaagcc
     3061 agagttcggg tggtcgacac caggccccgt gaggggaggg gaccggttat tgttagtgcg
     3121 tatattaaga gttccacaac ccgttcctcg ctgcaggtgg ccgtcgatgg cttatccgga
     3181 gcactcacct ccccggggca acccttgaag atccgggttt tttctgccac acaggaagga
     3241 cctcaccgcc cactttccgc aagacggcgg cagacgtgta gaggaacgtg agagaaaacg
     3301 taaatagagt tacggcccag tctctggatg gggatgtccg ggcggccttt ccaggtctga
     3361 gacccatcgg cggtgctcca ggcgggggtc tttacgagga taacaggatg gcaacagaat
     3421 cgatggtatc gggaaaagtg cagatggatc gggtttcccg ggggatgaag acagaacggg
     3481 tcttcattgt ggcgcggaaa gaattcaccg accaggtcac gggctggcgg ttcctggtga
     3541 tcctcgccct gttcctcgcc atcgccctgg tggggacgta cagcggggtc gtgagctacg
     3601 agagagaact ggatagatac tccgagcaac tggccgcgat ggacgacagg ttcgacggac
     3661 cggaacgaat gatgcccaca aaaccgccgg tcgcggatat ttactcctcg atgtttctca
     3721 cgctgatctc ttgcggaggc ctcctcgcca tcgccgtcgg gttcgacctg gtctccagag
     3781 agaaggagtc ccggtcactc aaaagcattc tctctcaccc ggtctaccgc gacgagatca
     3841 tcaacggcaa ggcgctcggg ggcatcgcac tgctggccct cgtcgtcgga ggagtgctcg
     3901 ccatatccac cgcgctcctc ctcgtcttct cgatcgtgcc cgccccgggc gacctctgga
     3961 tggtcctgac ctatgccggc gtcacccttc tcttcttcgt gaccttcttc tccatcgcgc
     4021 ttgccatctc caccctctgc cgggagagcg gcagcgccct gcttctttcg atgatcgtct
     4081 tcatcttcct cgttcttctg gccccttacg ccaccacgca tatcggcatg gcgttcatga
     4141 tggaagagcc cgaccctgcc gcatacggcg gggatatctc gtcagaagaa ttccaggcag
     4201 cgatcacggc atattacggg caattcaacc tgatccagag catcacgaac ctcgtatcac
     4261 cacaaatggc gaccaacgcc ctcatccagg ggatcagcaa ctcctccggg gcgaccctcg
     4321 aggatatgct cggcaggata tggtcgagta ttgcggcgct gaccatctac cctgtgatat
     4381 tcttcgctat cgggtatacg aagttcatgc ggatggatat ccgatgaccg ctcatgaggg
     4441 agggggccat gtatcccggc ccctccgtaa catcgagaga aaatcgatcg aaacactcca
     4501 atgtgtaaat aatcatggca gaggctattt atggggatgc gccgaccatt catcaactaa
     4561 taccgagccc ggcagcaaaa ccgcatcccg gacggtgcaa tatgtcaggt aacatcatcc
     4621 accccctccc ccgaagccgg ttctcgccgg cactccctcc gctcgtcgcg gccgttacgg
     4681 ccgttatctg ggcaggtacc gcaaggatcg tctccggaac gccgggcccg gccccaccgt
     4741 tcgtcctccc cgtcttcatc ctcgtgatcg tcgccctggt ggtcgcgggg gccgtaacac
     4801 cgctccccct gatcgagggc gccgtcccgg tcaaaaggca acggcaggca atgctcgtcg
     4861 ccatcgcagc ctcggtcccg ttcgtcggcg ccctggtgat aaaccccgag cggtggccgg
     4921 gcgggcctgc tcccctcata gcggagagcg tgccgatctt cgggtggttc ttcgacggca
     4981 tcatgagtgt cctgccgctc gccgcagata cgcccacgta taaactcttc ttctccgcgt
     5041 tcgcagtctt cgggttctac ctcgagtgcg tgctcgtcgc cgcgatcctt tacgttgttc
     5101 tccgggtgct cgccgccctc gatccgggcc cccgtgggga aagccggccc gaggaggagt
     5161 gaaccctcag aagaacgcga gagtcaggaa gtagaccccg atcaggacga tcgcgatgcc
     5221 gatgatccgg tcaaagactt ccccgctcat ccgcgccttc tccttgacct tcttcccgac
     5281 cgcgcccccg accgagctga gcacgatgag cgggatcgag agccccacgg catacacgaa
     5341 gatcgtgaag agtccctcga tagccgtctg gtagagggcg atgtaggtca ggacaataag
     5401 aagcatcggc gccccgcgcc ccacggtgat cgctccgaac gacaacccga gcaggaacgc
     5461 gcccgtgacg gagtaggaga taggcgcccg gaaaaagttg aatatcctcc gtatcggcgg
     5521 tttatacgca tgcaggagct gcagccccat caggatcatc agcaccccgc cgatagccca
     5581 cgcgatcgtc tcgtcgagga agaggaggta ctccccgagg taccccgcta taaccccgag
     5641 cgggaggagg acgaggatgg ttccgaacga gaacgctatg gtcagcctga ggatcccggc
     5701 aagcgtaaga tccgtctttc cgctcgtcag gtagccgatc agcccaagac aaaggacgct
     5761 gttgcagggg caaatgccgg cgacgaggcc gaagacgaag accccgagga ccgaggggtc
     5821 gaaggttgcc acagggaaag gttgggccgc aaccgggatt atacctgcga tccttttgcc
     5881 gggccattgc aggcggcgcc ctggataccc acttcacact ccaggtcggc atgtcaaccg
     5941 ccgccgggca tgcagagcgt tgcatgaggg ggtgaggata caggggcggg cgttgccgca
     6001 tcgagcacga agatgaggta taatgcggcg ctccccgtga ggcaacatat ggcgcggcat
     6061 acggggtgcc gtcccggcga agaacgcact gctcgccccg gtgaaatccc accgaaagca
     6121 ttgagagagg tatttcaaat cagagaacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnncg ccccggtgaa atcccaccga aagcattgag agaggtattt caaatcagag
     6301 aacctatatt tatagaacaa taatggtccc ctgggaaata gccttcgccg tcgtacctgg
     6361 cctgattctc ttcttctatg gaatcgagaa cttctcccgc gaaatcctcg ccgttgcaaa
     6421 aggcagcttc gcggcaatcc tcggcagggt gaccgagcgg ccgatcctgg ccgcgatact
     6481 cggcgccgtc gtcactgccc tcgttcagtc gagcgcagcg acggtgatca tcaccgtcag
     6541 cctggtcaac gccgggacgc tctcgttcgt ccagagcctc gggatcatca taggtgcaag
     6601 cgtcggaacg accatgaccg cccagctcgt cgccttcaag atgacggcgt tcggacaggt
     6661 gctgatcccg atcggtttcc tcgtcgggat cttcgggcgg cgttaccggt tcctcggaaa
     6721 acctctcttc tacttcggcc tcgttttctt cagtctgaac ctcatatcga cggcgctcgt
     6781 gccgttccag aacgatcccg agatcatcgg cctcatcacg cggttctcgg cgctcccgct
     6841 cgccatcctg atcgggtttc tcgtgacgac catggtgcag tcgagcgccg tcacgaccgg
     6901 ccttgccgtc gtcctcgccc agcaggggct gctcaccctt ccgcaggcga tcccgctcat
     6961 actcggcgcg cacatcggtt cgaccacgac ggccctcatc gcctccgccc ggatgaacct
     7021 ttactcccgg cgggcggcgg tggcccattt caccttgaac ctcgggggag tgctcctgat
     7081 cctcccgttc attgatccgt tcaccgaact catcatgacg atcggcggaa ccgaggcgca
     7141 gatggtggca aacgcccacc tcatatccaa cctgatcacg gcgacggtct tcctcatcct
     7201 catcaggcag ctcgggcaat tcgttacgtg gctggtgccc gggagcgagc ctgagatcct
     7261 gatgcggacg cactacctcg aagacggcat ccccgacgat acgcaccacg ggtttgagct
     7321 gatccgaaag gagctcgggt acgcccacgg ggtcacgctc gacctcttcc gcgcggtgat
     7381 gacctacctt gcgacgtcag atgatgcgga ttaccagagg gtcgagaggc tcaaagacct
     7441 caacagccac cttgacggga agatagggca ggcgcttcgg gagatatcgc ggaggcagct
     7501 tgcggaccag gaagcgaccg aggtggtctt cctggtccgg atgtcgcacg agatcggccg
     7561 gctcggctac ctcggggcgg acctcgggaa gttcttccgg aaagcatccg aaaacgggaa
     7621 cggcgccttt gtcgtcctcg cagagaagac agagagtgtc taccggatcc tcgacgcaaa
     7681 catcgtcggg ctcggccgcc acttcccggt gatcgatgac gcgaccgtcg ccgaactccg
     7741 cgcccgcgat gtcgagctcc agctcgccat caactggcac taccgcgagc agctcatctg
     7801 tctccgcgag aagggcgacc gcggggacag ccgctacctc gatgccctct ccatcatcga
     7861 ggcggcgaac gccaaagtgc gggatctccg caagctcgcc gagcagtact cgcgcgagaa
     7921 gatggatgaa ccggtgtcac tccgggtgga ggacccgctt cctctgcgcc acccggtagc
     7981 cggagacgac gagaagagag tccccgatat cccggtcgag cgccggatcc ccggaatcga
     8041 catacaggag gggtgtcgcg gcgagttttc cgggcgtggc gaccacgacc aggttcgata
     8101 acccggtctt ccggagcacc gcgggactga tctgctggtt ccccctcccg agaacgaacc
     8161 cctgggcgcc gatggggctc acgacgatct ttgcccgcgg atatcggtcg aggagggcaa
     8221 ggagggtccg ctcgtcggtg ttccgggcga cgacccgccc atccctgacg acgtcgacgc
     8281 caagaagcgt cggggttaga ccgagccgct ccatgatccg cgccgtcgtg ctcccggcgc
     8341 cgatgatata aagcgtctcc gggagcatga cctcattgat gaacgccgcg atatcgtcct
     8401 tcgcccgctc ctcgtcctgc tgctcaaaga cctgttttcc cccttgagtc cgctccggta
     8461 tatacgggac acgcaggtac ccataaagcc gggcagaaag ccggcccgag cggtaggctt
     8521 cttcgtcgac gtccatcacc tcggagtcgc ggcaggcaac cccccccgcc cgccggatga
     8581 ggtcggccgc ggccgccggg ttgactgcaa agaccgccga gtacatcttc acaccggcag
     8641 ggatcccgag gacggggacc gaccgcccca cggcgtcaaa gacgtctcgg gcagtcccgt
     8701 ccccgccgca gaagacgatg aggtcgagac cggcatcgag aaacgcccgg cacgcggcct
     8761 tcgtatcggc agcacccgtc gggccagatg gccggtagac gacggtaaaa cggtcgatcc
     8821 cggcaccggc aagggcatcc tcgcccatcg gcgcggcaca ggtataccac gtaatatcgt
     8881 cgtctctgag aagggagagg gcctggaccg cccggtcgcg ggcgcggggg acggcgccgc
     8941 gcaggagcgc ctccgagacc tttccatccg tccccgcgag ccccacggcg ccgcccatac
     9001 cggcgacggg gttgaccaga aacccgacgg tctttctcat ccctctactc tttcgcgccg
     9061 gggcatatca ggatcggggt cgggagcgaa tccgctcttg agcgccctga ttacggcgcg
     9121 gaggacttcg tgcatcccgg cttcgtcgcc ggggtgctcc tcgattacgc ggacaagcgc
     9181 gctccctacg atgacgccgt ccgcaccggc ggcggcgacc gcagccacgt gttccgggcg
     9241 cgagaccccg aacccgacgg cgagcgggag gtccgtcttc tcccgcaccg caccgaccag
     9301 cccggcgagg ttcgggggga gatggtcgcg ctcgccggtc accccctcaa gcgagatcag
     9361 gtagacgaac ccggaagcgc ccccgaggat cttttgccgg cgctccggcg aggtcgtcgg
     9421 ggcgacgagc acgatccggt cgatgccgtg ccggagggcg gagggtgcga cctcgcccga
     9481 ctcctcggtg gggaggtcga cgatgaggat accgtccgca ccggcggcgg cggcctccgc
     9541 aaagaaccgg tcgacccccc ggcggtagac gatgttgtag taggtgaaga ggacgagggg
     9601 gaggggcggc gcgtactccc tgacccgccg aacgagggcg aagacgtcgt ccggggtggt
     9661 gcccgcccgg agggcgcgga cgtgcgcccg ctcgatcacg gggccgtccg ccaccgggtc
     9721 ggagtagggg acggcgatct cgaggaggtc gacgcccgca tcgaccatcg tcttcgccac
     9781 gccaaacgac gtttctctat cagggtcccc ggcgacggtg aacccgacga agaccgggtt
     9841 cttccgggcg aaaagtgcct cgatccggct catgccgcgc ctccgtgaac gttcgcgacg
     9901 tctgcaacgt ccttgtcgcc ccggccggag aggttgacga cgaggatgtc gtcttcatcg
     9961 aggttgtccg ccgcccggag ggcgtaagcg accgcgtggg cggactcgag cgccgggatg
    10021 atcccttcga tgcgggagag gtagcggaag gcgtggagcg cttcggcgtc ggtgaccgcc
    10081 tcgtaacgga cccttcccgc gtccttcagc atggcgtgct ccggcccgac ggagggatgg
    10141 tcgaggcccg cggcgatcga gtgcgtctcg agcgcctggc cgtcgccgtc ctggaggagg
    10201 taggagagcg ctccctggaa gaccccgacc gaaccggccg agagcgaagc cccgtgacgg
    10261 ccggagataa gtccctcgcc gcccgcctcc acgccgacga gccgcacatc gtcgttcacg
    10321 aacgggtaga agatgccgat tgcgttcgaa cccccgccga cgcaggcgac gacggtgtcg
    10381 gggagccgcc cttcccgttc gaggatctgc cgccgcgcct cctcgccgat gaccgactgg
    10441 aagtcccgga cgatccgggg gaaggggtgc gggccgacgc aggagccgag caggtagtgg
    10501 gtatccccaa aattcgccac ccagtcgcgc atcgccgcgt tgatggcgtc tttaagtgtc
    10561 cgcgtcccgg aggcgaccgg gacgacccgg gcgccgagga gctccatccg aaagacattg
    10621 agcgcctggc gccgggtgtc cacctcgccc atgtagacct cgacgggcag atcgagaact
    10681 gcgcccgcga tcgccgtcgc gacgccgtgc tgcccggcgc cggtctcggc gatgagccgg
    10741 cgcttgccca tgaacttcgc catgagcgcc tggccgaggg tgttgttcag tttgtgggcg
    10801 ccgccgtgga ggaggtcctc ccttttcagg tagacgcggc agccgaggtc gcgggagagg
    10861 ttcccgcaga aggtaagcgg tgtctcccgg ccggcgtact cgtgcaggta ccaggagagc
    10921 cggcgggaga actccgggtc ctcgcggacc cgggcatagg tctcctcaag ctcgatcaac
    10981 gcgctcatca gggtttcggg cacgtactgc ccgccgtata ctccgtatcg tcctgctctc
    11041 atggttctat cgtcctgcat gccatcacga acgccctcat gagacgctcg tccttgatcc
    11101 ccggcgccgc ctcgacgcct gaagcgacgt cgacggcgta aggccttacc gcccggattg
    11161 ccgcggcgac gtttccgggg gaaagccccc ccgcgaggat gaccggcacc ggggaggagg
    11221 ccacgcatct tcgtgcgtat tcagggtcga acgcccgccc ggtgccgcgg ctgccgtcga
    11281 cgaccaccgc gtcgcagtcg tcccggagag cgtcgccggg agcaagcatc cggatgaccc
    11341 ggacccccgc gccccggggg agggcgaggt cgcctgccac ctggaccgcg tccggccgcg
    11401 aggagaggat cgccgcgagg tcctcctccc ggccggtcga ggtgacggcg accgtggtca
    11461 cgaacggcgg aaccgccgcg aatatttccc gcgccagctc cggggtcacc gaccggggcg
    11521 aggggctcgc gagcaccacg ccgatcgcat cggctccggc cgcggcggcg gcacgggcgt
    11581 cgccgacggt ggtgatcccg cagatcttca cgcgaatacg aatccctcca gccgtttgca
    11641 ccgatcttta gccgacatca gggcggaccc gatcaggaac gcatcgcaat gcctcccgag
    11701 gctccggaca tcgtagggcc acgtgatgcc gctctcggag accacgatcc tccccgcgtc
    11761 gcgtgccgct tccgcgaggc ggaaggtcgt cgagaggtcg atcctcatcg tctccaggtc
    11821 gcggttgttg atcccgataa gtctcgcatc cgtcgcgaga gcgcgttcca tctcgctcct
    11881 gtcgtggacc tcgacgagcg cctcaagacc gagcgcgtgt gccccgtcga cgaacgcggg
    11941 gaggcgatcc ccgagcaccc gggcgatcag gaggacggcg tcggcgccga gcgcccgggt
    12001 ttctgcaagc tggcgctcgt cgacgatgaa gtctttcctg aggatcggca ccgttaccgc
    12061 acgccgcacc cggacgaggt tttcgatgct cccaaaaaaa taggcaggtt cggtcagcac
    12121 cgagagcccg acggctccgg cggcggcgaa ctccctcgcg atcgtctcgg gggcgcagcc
    12181 gtcgtggatt ctgccgcgcg acggggaggc gtacttcacc tcggcgatga tcgcgcgtct
    12241 ctcccggcac gcacgtatgg cggcctcaag gctccggtgg ggcggagcgc cgggatcgat
    12301 ctccggaaga gtgtcgaggc tctccaggcg ctcccgggtc gccctgacga tatcgtcgag
    12361 gatcataccg ggcctccggt cgcctcgacc agccggcgga gccgatcgag cgccgcgccc
    12421 gagtcgatcg aagcctcggc gcgggcgatg ccggcggcga tgtcgccggc ctttccaccg
    12481 aggtagatcg ccgccccggc gttgagggcg acgatgtccc gggcggggcc gtcctccccc
    12541 gcgaggacgg agaggaggag ccgggcgttc tcctccggcc cgccgccccg gagtgcggcc
    12601 acaggcgagc gcgggatccc gaactccatg cagtcgagcg tatacgtttc gacctcgccg
    12661 tcccggagct ccgcgaccgt cgtcggcccg gccgtcgcga tctcgtcgag acccgcgccg
    12721 tggaccacca tcgcccgctc caccccgaga tccccgagca cccgcgcgac cggaagggtc
    12781 aggcggggat cgtagacgcc gaggaggtga gccccggcac ctgccgggtt cgtgagggga
    12841 ccgaggaggt tgaagaccgt ccgtatcccg atctcctgcc gggccgacct ggcgtactgc
    12901 atcgccgggt ggtaggccgg ggcgaagagg aagacgatcc cggcgaccgc gaggacctcc
    12961 ccgaccttat cgggcgggat cgcgacggag acgccgagcg cctcgaggac gtcggccgaa
    13021 ccgcatcggc tcgatacgcc ccggtttccg tgcttcacga ccgggacgcc ggccccggcc
    13081 gcgacaaagg cggccgcggt gctgatgttg aacgtccccg caccgtcgcc cccggtcccg
    13141 caggtatcca cccgcatctc cggggcgggg agaacaaccg ggacggccgc cgcccgcatc
    13201 gcccgggcga atgccgcgat ctccgtctcg ctctcccctt tcatccgcag tgccgtgagg
    13261 aacccgccga cctgggcggg agtcgccgcg ccccgcatga tctcctccat cacccttccc
    13321 gcctcggcgg aggtgaggtc cgtgcccgtg gagatccgga caatcgcttc gcggatcatg
    13381 ccgcaccccc cataccggag aggaagtttt tcatcaggcg gttcccgtcg ggggtgagga
    13441 tgctctccgg gtggaactgc accccctcga tcgggaactc ccggtgccgg acgcccatga
    13501 tcgccccgtc gtcgtcgctt cgggccgtca cttcgaggca gtccgggacg gtcgccggat
    13561 cgatgacgag cgagtggtaa cgggtcgcga cgagggggct tgccaccccc gtgtagatcc
    13621 ccgcaccgtc gtgccggatc atcgaccgct tcccatgcat cagccgatcc gcccgggcga
    13681 tgcgtgcacc gaacgcaagg ccgatcgcct ggtggccgag gcagaccccg agcgtcggga
    13741 cggtccggct gatcgtgccg aggacctccc ggtagagggc ggagtcccgg ggatgccccg
    13801 ggcccggcga gaggacgaca cggtcgaaga gcacgggacg gatccgctca aacagcgtat
    13861 cgctcttcac cacgaccggc tccgccccga gcataccgat ctgctggcag aggttgaagg
    13921 tgaagctgtc gtaactgtcg atgacgagca cccgcatcac tccgcctccg cgccgagcgc
    13981 cgcaagcatc gcccgccctt tgttctcggt ctcggcccac tcccggccgg ggtcggagtc
    14041 ggccacgatc cccgccccca cctggacgga ggcggcgccg tcctccacga cgatcgtccg
    14101 gatggctatc gcgaggtcca tcgtgccgga aaaaccgagg taaccgacgg ctcccgcgta
    14161 gatcccgcgc cggagcttct ccgtctcgcc gatgatctgc atcgcccgga ccttcggggc
    14221 gcccgaaacg gtccccgcgg gaaaacagga gcgtaaggcg tcgaatcggt cgagcccctc
    14281 ccggagggtg cccgtgaccg tcgaggcgat atgctgaacg tgcgagaacc gctcgatggt
    14341 catgaacccc tccaccgaga cgctcccgta cctcgagacc gccccgacgt cgtttctcgc
    14401 caggtcgacg agcatgatgt gttcggctcg ctctttctcg tccgcgagga gctcggccgc
    14461 gagccggtcg tcctccgccg gggtccttcc ccgcggccgg gtgccggcga tcgggacggt
    14521 cgtcacccgg ccgtcctcca cccggacgag catctcggga ctgctcccgg cgaccgccat
    14581 gtccccgaag tccaggtagt acatgtaagg gctcggattt ttcacccgga gccgccggta
    14641 gacctcgaac gggtcgcccg cgatacggca ggcgagccgc cgggagagga cagcctggaa
    14701 gacgtcgcct gcggcgatat gctccttgat ccggagaacc gcgcccgaga actcttccgg
    14761 ggtgcaggac gacgtcacaa cccctgagga ggggggatag cccgtgcatg ccggggtgag
    14821 gtcgcgtatc cgggcgagcg ccccggcgat cgcctcacgg gcccggtggt actcctcctc
    14881 tgcgtcggct ccgtcggtga cgagcaggtt cccgacgacc gcgagccgtt ctctccggtg
    14941 gtcgagggcg aggcagtttt gcgccagcat gaaccgggcg gcgggctctt cggagccctc
    15001 cgccgtctcc acccgggaga ccgggtagat ggaagagatg aggtcgtagg agaagtagcc
    15061 cgcgagcccc ccggagaacc cccggagcgg cgaaggggcg acccggaacc gggccatgaa
    15121 cgaacgggcg gcaccgatgg gatcggcggc ctcgacctcc ccggcgatcg ctcggatgcg
    15181 gggatcgccg gagacggtca gcgcaccgtc ggaggccacg acgaccgtcg cagccggggc
    15241 ggtgcagatg aaggagtaac gggcggtctt cccgctccct tcgagcgact ccagcaggaa
    15301 cccggggcca tcccggaggg acgcgtaaag gtccgccggc gaaggtgccg gaagcgggat
    15361 ctcggtaaat acggggacga tgagaggcag acaggccgcg tccgctgcag cgacggccgc
    15421 ttcgtacccc ggttcggtgt tcagctctct tggagcgccg tccagtccac cggcatcacc
    15481 ccgtcgccgg cagccgggcg agcgactcct tgatcagctc gaccgggtac tcgtagtcga
    15541 cgagccttcc tccaaagtat gcctcgtagg aggagaagtc gaagttcccg tgcccggagt
    15601 tgttgaagag gatcgtcttt gcctccccgg tcgtgcggca ccggacggcc tcatcgatcg
    15661 cggccttcac ggcgtgggct gcctcgggcg cgacgatgat cccctccgtc cgggcaaaca
    15721 tcacggcggc atcgaagacc tcgttctggt ggtaggcaac gggccggacc acgccatcgc
    15781 gggcgaggcg ggagacgagc ggcgacatcc catggtagcg caggcctccg gcatggatcg
    15841 ccggcgggac gaagtcgtgg ccgagggtga acatccgcag catcggcgtc agccccgcga
    15901 catccccaaa gtcgtaagcg tagagcccct tcgtcagggt cgggcaggcg aacggctcca
    15961 ccgcgacgat atcggtctcg gggtgttttc ccgtgatctt atcgccggca aacgggaagg
    16021 agagcccggc gaagttggaa ccgccgccga cgcaaccgat caccacatcg gggtaggctt
    16081 ccaccatggc aagctgctcc cgcgcctcct ggccgatgat cgtctggtga aggcagacgt
    16141 ggttgaggac ggagccgagc gcgtagttgg tgttctcgtg gtttgccgca tcctcgaccg
    16201 cctcggatat ggcgatgccg agggagcccg gcgtatccgg ggagtgggcg aggaccgccc
    16261 gtcccgaccg ggtcttatcc gacgggctcg gtatgcattc ggcaccgtag acctgcatca
    16321 tgctcttacg gtagggtttc tgctcgtagg aggaccggac catgtagacg gtgcactcca
    16381 tgtcaaagag gcccgctgca aacgcaagcg atgaccccca ctgccccgcc ccggtctcgg
    16441 tcgcgaggcg ctcgaccccc tcctcgcggt tgtagtaggc ctggggaatg gcggtgttgg
    16501 gcttgtgcga gcccggcggg ctcacgcctt cccacttgaa gtagatcttt gccggggtct
    16561 tcaaggcggc ctcgagcctt cgtgcccggt agagggggct tggtctccag agggtgagga
    16621 tatcccggac ctcgccgggg atatcgatgt agcgctccgt agtcatctcc tgccggatca
    16681 gttccatcgg aaatatgtgg cggaggtcat cgggaaccgc cggtttcccg gtcgccgggt
    16741 ggaggggcgg atccatgggt gtcgggagat ccgcctggat gttataccac cgcttcggca
    16801 tctccccctc gtcaaggagg atcttcgtct gcatacacca tggtgtgttt ctgacagata
    16861 tatacattgc ggatcaacaa tgatcagagt agtttgtttt aatgcgcatg gcccgggcgg
    16921 ggataggccc ccgggaaaaa gggtatatgg taatatcacc actggagagc gatgtacctc
    16981 ctcgcccacg ccatggtcgg catcctcatc gggatcgtcc tcgcggcgat cgtcggcgat
    17041 cggcgactta tcgcgctcgg cgccctcggt gccgtcctcc ccgacctgat cgataagccg
    17101 ctcgggcaca tcgtccttgc cgggacggtc aactacgggc ggatctactt ccacggcctc
    17161 accgtcctct ccctcgccct cctcgccggc ttcctcatct accactaccg cggccaggtc
    17221 ggcctcctcg tggtggccgc cggtatggca agccaccagg ttctcgacgg catgtggcgg
    17281 cagcccgtcg agtggttctg gccgttcctc ggccctctcc cacagcacga ctacccggga
    17341 gactacttct ggagctcgat cctgcgggag ctcgcccagc cgagcgaatg gctcttcttc
    17401 ctcctgatcg ccgggctgtt taccgttctt taccggcggg agataggggc cctcctcacc
    17461 gggcttgcag accctggaac ctggaggatg ctcgtcctcg tcctcggtct cgccgccgtc
    17521 gccgcgctgg tcgtgggcgc gcggttcctg tgagagaaga tctcccggcc ttcacccctg
    17581 tttttgccac cggatcgcgg cgtatatcga tctcccgggg agggcaagcc cgccatcctg
    17641 ccggatgagg tgttcacgga agtagtcccg gagtatctcc cgctgccgca gggtcgttac
    17701 gcccatccgg ggagtgaagt ggtcgaccgc atcctccacg gtcgtgaagg cgaccgtatc
    17761 gcccatcggg cgcatccgga cgtctgcgta gatccccatc ccgtagagga cgttgaagag
    17821 gcagtcggcc ttcggcctcg ggtgatacgg cgccccgtgc aggtcctccc agagcgcgag
    17881 gtatatccgt tcccagagcg gttcatcggc gaaccagtag aggtggaccg acccggactc
    17941 ggaggcagct gcattcatcg ccgccgcggc cgaccggatg tcgggcatgg tgagcgagaa
    18001 cgaggcgacc accctgtcgt aaggcggcgc gatgtcgcag gccggatcga tctcctccca
    18061 gaggccgcgg acggtggtga tcgtggtgat cccctcctgc tccgccgccg cccggagctc
    18121 ggcaagcatc ccctccgcag gctcgactgc cgtcaccagc gcaccggccc gggcaagcgg
    18181 cagggcgaga gtcccgggcc ccgacccgat atccagcacc cggtcgccgg gagcgacgtc
    18241 gaggtccgag agggtctcgg caacccgtcg tgcatgatcg gacgcaaggc gggtagcgta
    18301 ccgccgggcc cgctcccggt cgctccagag ttcgacaccc tcccggaacg ccggagtcga
    18361 ccggttcagg gcaagcctcc tccgccagac ctcgttccaa tccggcatgg gaagatcctt
    18421 catcggacaa tcggtcgcct ccgaagcata aacaggtatc catacgcgcc ctgtgggcat
    18481 ccgtgtggcc cgggggatac cccaaccatt atatatgagg ccggagaggg aatacttgtg
    18541 gtgaatagtg tgactggcga atccatgctc cagataaccg tcgatcaact tgcgagatca
    18601 ctctgtgcga gcgccgtcct cggtgccccg gtcgagactg ctgaacgggt catcataccg
    18661 gtcgccgaat tcgggttcgg gtttggtgcc ggagaatgca aaggagggac gaaagaaggt
    18721 aaccagggcg gcggaaccgg gaccggcggc gggggcggca tatcggccat cgctctggtc
    18781 atcgtcacca agggcgtatc cggccccgaa ggtattcagg tcatatcgct gaagaagaag
    18841 agcgagatcg ccgaggtgat cacgacgctc ggggaggcgg tcggcccgca tgtcgnnnnn
    18901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngggga cggcaaccgt agcctggggc
    19021 atcgtggggg cgagggtccg ggccgacgag gatgtgcagg tgttcgaggt cctccttgcc
    19081 ggccggcggg tcatggtccg ggatatccgg gagatggcgg cggcaaaacc ggaagaagag
    19141 aagaaggtgg aggagccaaa accggccctc ccggtggggg agtacgtcga cgccgcccgc
    19201 gacctctggc cccatatcca gaggatcgtt gccgccatct accggtctct ctaccttgag
    19261 accctccgcg gggaggtcac cctcggcctc gagagccccg ccagtaccgg cgtcgtctac
    19321 ggttactgca ccgccgcccg ttacgccctc tggccggcgg aggcgatcga tctcgtgctg
    19381 accccggact ttgaccatga agtcttcgag ggaacgttca cgctcagagt gcagatccgc
    19441 cgccccctcc tgatcgtcat accggtcgtg caggctctcc tgcagaaacc ggtgcggcaa
    19501 cggctccggc aggtctcggg aagaggtgct cccggtgcgt gaggagactg aacttgttat
    19561 cggcggcgcc cggagggccg gcgaccggta catcatcccc gtcatcagga cgctcgttct
    19621 ccatgcagga agaactgccg tggtcagcct gacgccggtc gcgctcatca tagccgaagg
    19681 agagtgcgag tacctcatcc gcctcccggg cgcgccccgg tcgatcgaga gcgcactcga
    19741 tacgcttcgc ggcgatatcg accgccaaaa aagagagtgt ccggggtagc cgtcactcgt
    19801 ggcggagagc ctggatcggg tttaagtgcg ccgccttcca ggccgggtag atgccggaga
    19861 ccgcgctcat cccgatgccg aaggccatcc cgaagacgat gtagagaagg cttgtcggat
    19921 tgaagaagta gtcggggttc ccggagaaga cagccgtcat catgtatccg gtgccgaaac
    19981 tgagcacgcc cccgatggcg gcacccacga gcccgaggat cagggcctcg tagatgaaca
    20041 tccgcatcac ctcgctccgg cgggtgccga tgctccgcag gaccccgatc tccttgatcc
    20101 gttcggtgac cgacatcagc atcacgttta agatcgagac tccggcgacg atgagcgata
    20161 ccgcgccgat acccatcaga aagaccgtga tcgagtcgac ggccgcaaag acctctttca
    20221 ggatcccccg ggtatccatg acggtgacga cattctcccg ccgattcatc ttctgctcga
    20281 tcgcctcctt gaccgcatcg aggtcgttga cgtcgcggac cttcacgatg acctggtcgt
    20341 acgtatcttt atcgtagtgg ttcgagtacc acgagtaggg cacgacgatg gcactatcag
    20401 ggttgatgtc gaaccccatc ccccgttcct cgagcacgcc cacgacccgg agggtctcgt
    20461 tgccgatcgt ggcccgggca ccgagtttga ggccggtatc cttgctgttc tcggcaagcg
    20521 tctttccgat cacgcacccg gaaccggtat ccttcgggta tgctcccgcc tccatatcga
    20581 gcaggaaggg gatatctttg gccgggatcg cgtagatcat ggcgccgctt ttggtctcgc
    20641 ccacggttac cgagtcaaac gtggtcgcga acggtatggc ccggttggcg ccgaccgccc
    20701 gggtgatatc gtccacctgc cgtttggtga gttccgtggt tcccgagcgg gtcgccgggg
    20761 agacggtgat ggtgtcgccg acatcgctga ccatatcgtt gaacatgagg ttgatgctgt
    20821 tgcccatgat accgagcgtg gcgatcgcga cgacgccgat ggcgatcccg gtgaccgcga
    20881 ggatcgacct gagccagtgg cggctgacgt tccggcgggc aaggtcgaag aaggtcatgc
    20941 cagcctccca tccaccagcc ggatcgtgcg gtcggcgtac tcggtcatcc ggggatcgtg
    21001 cgtgaccatg atgacggtct tgccctcctc gcggttcatc cggccgagga gttccatgat
    21061 cgccgtcccg gtcttcgagt ccaggttccc ggtcggttcg tcgcagagga ggaagtcggg
    21121 atcgttgatg agcgcccggg cgatcgccac ccgctgctgc tgtccgcccg agagttcgcc
    21181 gggtttatgg gtgaagtgcg actcggtgat tccgaccgcc gcgagaacct cctctgcccg
    21241 ctcgcgggat acatcccgcc ggcctttcag gatgagcggg tactcgacgt tctcgaccac
    21301 cgagaggagc ggtatgaggt tgaactgctg aaagatgaac ccgatgtggt cccgccgcat
    21361 aagggtcagt tcgtcgtcgt ccatcctgct gatccgcttt cctgcgatgg tgacctctcc
    21421 tgatgtcggg acgtcaagcg accccatgac gtttaagagc gtcgacttcc ccgacccgga
    21481 ggggcccatg atcaggacga actcaccctc ttcgatagcg agatcgatcc cggcaagcgc
    21541 cacgacatcg ccggccggga gggggtagac cttcgtgacc gcatcgaaac tgatgacggg
    21601 catcgctgtt acttccgtct ccaggagtag tagatggcgc cgacgatccc gagagcgaga
    21661 agcacgacga cgacgatgcc ggcaagcggc agctcgccgt cctctccgcc ggacagcgcc
    21721 gttgtggtgc cgccgacgtc gaccatcgtc gtcgccgtgt agcggttgcc atcagcgtcc
    21781 cggtactcga tgacgatcgg gatctccgtt acgtctgcgt cggcctggag tgtcacctca
    21841 aacggggacg catcgtcggg atcgagggtt ccgacgatgt agatccggta cggatcggtg
    21901 ggggtcgccg gctcgcccgg ggagatgata accgatcgcg cggactcaag gcctgcgttc
    21961 atgacatcgc ccacgatccg gtatcctttt gcttcctgcc ggacctcgac gttggtgatg
    22021 accgggtccg cctgccgttt gtcctcgtcg aacgtgatcg gcaggatgag gtcggcggta
    22081 tgggtgttga tgccgttgcg ccagaccacc tggaaggcgg cggtcgtctc cgctgccggg
    22141 gtcaggttga agacggcggt cccggaggcg tcgggcgcaa gagcgccgat gaacgcgctc
    22201 gacgggatgg ccttaagacc tgttccctgc gggatcacct ggacgccgga ggcggcgttc
    22261 gggcgggggt tccccacccg gatggttacg tcggccgtcc tcccctcgga gaaggcatcg
    22321 ggttttttga tcaccgatat gctgagcggg gtattctcga cctggaccgg gatcgggtag
    22381 cgcaggctgc cggcgttgac gaagtcgagg acgaagacgg ggtagaacgt cccctccccg
    22441 ccgtccgcac gaacggtgaa ggtgaacgtc ttcgtgtttt tggtaccgag ctccccgaca
    22501 gcggggtagg agtcgctcag cggcacgaca ccgctcccga agagccgggc gctccggata
    22561 gcgaccggtt cggcaccgtt gttctggatc gcgatcgtga gcgtcccggt atcccctttc
    22621 atcaggatcg gcgggtcgat ggaggaggag gtcacggtga tgtccgcggg gccggcagag
    22681 gccggtccgg tgaatgccgc gagggcgcag acaagtgata tgatgataaa gtagagtggt
    22741 ctcatggtta cagccatccg gcgagcgtta tgaggagata gatgatgtag aggcccacgg
    22801 gtatcccgac ggtgagggcc gcattccgcg tggagaggtt gcgcgcatgt ttcatgccga
    22861 agacccatat gttcgcgctc caggccagga agaggatggt cacgaccccg gcgatctgcg
    22921 cgagcgggtc ggttgctatg acgtgcgcga ggttctccgt caccgccgcg atctcttcag
    22981 ggctcctcgc gatcgggagc gtcaggttcg ctatgatctg gtaggagaga agagccccga
    23041 tgatgccgcc gaagatctgg ggcaggaacc cgtaaccggt gacctcgagc gtccgcgcaa
    23101 gcgatccctc tcccttgaag gccatcgata tgatgtagaa gacgatgccg tatatgaacc
    23161 atgtcaaaaa ccccccgacg acggcaacca ccgcggcgaa gcccaccatg agcaccccga
    23221 tggcctgggc ctctgcgggg agtattccaa cgaacatgtt tgccgccagt gcagccgaga
    23281 ccgcaccgat caccccgata acgagcgcga tcagcgccgg caccttcagg ctcggttcat
    23341 cccgcatgcg gctctcgaaa aaacgcccgg gatcgaagag tacccggaga aatccaattt
    23401 ccatgataga tatgcctcct gtatttctgt tatacaagag gggataaact ttttcggaaa
    23461 cacggaagca aaaattcagg gatcttccca aatctccgga gaattgcgag ggtattgccc
    23521 caaacccgga cctgaggcgg accgcaccgg gatcgaggtg ccggcgagga tatgccgccc
    23581 gcatcgcccg ggggaagagc ggcggcgggg acctccctga cgcctaacgt ctcaaaatcc
    23641 cgcatgcggt tcttcttcgc ggcaacgcga gaggctgtct caccgctcat atacctgaac
    23701 acgcgcattg ccgcgaagtc cgggagatgg gtcgagcact cccctacatg tgcccgagct
    23761 ctttgaggct cacgtaaccc ttcttcgtga cgatgatgtg gtcgagcacc cgtatgccga
    23821 ggatcgaccc ggcctcgatg agctgccggg tgatgctgat atcctgggtg gaggggtcca
    23881 gggtgcccga gggatggttg tggaccagta tgaccgatgc ggcgcggtcg gtgatcgcct
    23941 cggcaaagac ctcgcgggga tggacgaggc tctggttcag gataccgatg gtgacgaccc
    24001 ggcgtctgat cacctccccg gcaccgttga gcgttatgca gaagaaatat tcctgtttct
    24061 tgtcccgcca ctcgctgacc agcgggagca cttcatcggg cttggtgatc cggctcccgg
    24121 caacgccctc atcgtctgcc aggtatcgcc gcccgagctc gaagcaggcc atgatctcgc
    24181 aggctttcgc cgccccgatg ccctctatca cgaggagggc atcatacgag ggattgcctt
    24241 tggcattatc caggtattgt tttacatctc cggcaatctg cgagacgtcg cgccccttta
    24301 caccccgccc gaggaggagg gcaatcagtt cggcattggt gagcgactgg ggcccccgtg
    24361 ctgcgagctt ctcacgcggg cgatcgtggt cgggtatatc gcgcatcttc ttcatgctca
    24421 ttcggccctg cgaggttctt catctccggt tttgatttgg aagcatgtat acgttgtcaa
    24481 tcagttcgcc gcaccgggtg agcgcaacgc gagagcaggg cgaggggcgg cgttaggttc
    24541 tctgttgagc atgcagaaaa ccgggggata ctaccgaaac ccttttgtct gagaactgct
    24601 atctctcaca aaatggagct gccctggagg agcaccgagg tctggtgcag gaaaggacgg
    24661 gcgtattgcg aggaggggca gtacgaccgg gccgtcgagt gctacgaccg ggcgatcagg
    24721 ctgaacgccg gcgccgccga tgcctggtac cataaggggc tcgctctcac cgctgccggg
    24781 cgccaccagg aggcggccgg atgtctcgac caggccacca ggatcgatcc ggcatccgcc
    24841 cgctcctggt gcgcccgggg gcaggtgctc tacaacctcc ggcagtacca ggaggccgcc
    24901 gagtgctgca gccaggcggt gaagctcgcg cctgactccg cggacgcctg gcttacccgg
    24961 gggcacgcct tcaggagtat ggggcgaacc cccgatgcga tcagaaacta cgatcaggcc
    25021 atcgccatcg atccgggccg ggtggagacc tggcttgcca ggggaacggc gcttgccacc
    25081 gaacgacagt atgatgcggc gatcgactgc tacgatcagg tcatcgcgct tgaccccggg
    25141 aacgcaaacg cctggtatgc cagaggaacg atccaggcca tcctctcccg gcacaacgac
    25201 gcccttgcat cctgcgaccg ggccgtcgcg atcgactcaa accacgccga cgcctggtat
    25261 gtcaggggat gcacgcttgc ggtgctggag cagtatgcgg aggcgatcaa ctcctacgac
    25321 cgggtgcttg ccgtccgtcc gggggatgcc gatgcctggt acaaccgggg ccgggcgctg
    25381 caccgtctcg agcgccatgc ggaggcaaac gagtgttacg accgggcgct ccggatcaag
    25441 cccgactacg ccggaatctg gtaccataaa ggcacggcac tgaggaaact gaaacgctac
    25501 gaaccggcgc tcgcctcttt tgaccgggca ctcaaggaga acgccgccga cccggggata
    25561 tggtatcagg aggggctcat cctcttcagc ctgaagcgct ttgagaaggc gatcgagtgc
    25621 ttcgaccagg tgctcaggct ccgcaccgat cacccggatg catgctacta ccgtggcgag
    25681 agttactacg ccctcgggga ctacggggcg gcggtcacct gctaccggac cgtggtccgg
    25741 atggacccgg agaacgccgt cgcctggaac aactacggaa acgcgctcta ccggctcgag
    25801 cgttacgaag aagcccttgc ctgctacgaa cgggcgcttg gagtcaaccc cgagaaccag
    25861 ggcgcctgga gcaacaaggc aaacgtcctc tcgatcctca cgcattacga tgaagcactc
    25921 gcctgctacg accgggagct ccggacaaac ccggagaacg cagacgcgtg gtacaacaaa
    25981 ggtcttgcgc tcttcatcct cggatgttac ggcgaggctg ccgcctgcta tgcgcgagca
    26041 ctcgctatcg aacccgcgag agcggaggtc tggtgcgccc ggggcaacac cctcgtcatc
    26101 ctggagcgtt acgaggaggc gctcgactgc tacgaccggg tgctcgccgc aaaccccgac
    26161 gaccccgggg cgctcaaggg gaaggcgatg gccctcatct acatcgaccg ccccgccgag
    26221 gc
//
LOCUS       DAOSII010000013        25620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_2152_length_25620_cov_49.137649, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000013 DAOSII010000000
VERSION     DAOSII010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 25620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25620
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2152_length_25620_cov_49.137649"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(312..722)
                     /locus_tag="PLV90_01990"
     CDS             complement(312..722)
                     /locus_tag="PLV90_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOF95914.1"
                     /translation="MIEVYPLLVLVVAVVLIAGCTGPAESGVIIRTVPPAGASALIDE
                     RGQDPAFAVIDLRRPDEFAAGHVPGAINIDSREFEGRLTDLDRNGTYVIYCQRGGRSS
                     GVREMMREAGFREVYEVEGGMSAWAVAGLPVTLE"
     gene            complement(775..1494)
                     /locus_tag="PLV90_01995"
     CDS             complement(775..1494)
                     /locus_tag="PLV90_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF95915.1"
                     /translation="MTVVTVEDLIRCLERLQARGPIPFDVKSGEVFGILCTCGKGRRI
                     LTAILMTTIFPVPALAERSPLAILGNLGDVRRSMGVVFPEPILDPALTARENLDFHAR
                     MHGLGEGARRRRIAEIARLFNLSGSLDATVETFPPAMARSLEIARAYLIHPSVLFLAE
                     PTKGLDGAGRRGIWEALQRLNRDRRPTIVLTTESLEEARAICHRVAVVDGGEVVALDT
                     PDTLCAVMGMDDAAPGFDDTA"
     gene            1829..2536
                     /locus_tag="PLV90_02000"
     CDS             1829..2536
                     /locus_tag="PLV90_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HOF95916.1"
                     /translation="MTTDISTIAILPVPFSPDQALIALAVLIPVLLLLILLFISAGVF
                     ESIGFRFYQAVLVTAGALFGSLVNIPLIPVGEAIIAVNVGGAVIPLFVTLDLVVRGRV
                     SRPKTLAAIAAVTLVAYMLATPVPGTGITMPFYVAPLAGAAAGLILAGGCHAAPGLAY
                     AGGAMGTLIGADILNLANPAVFAALTGGKAAVLSIGGAGIFDGIFVTGIFAVLLAAYA
                     GRQLRKSAGVCPREGRE"
     gene            complement(2559..4361)
                     /gene="asnB"
                     /locus_tag="PLV90_02005"
     CDS             complement(2559..4361)
                     /gene="asnB"
                     /locus_tag="PLV90_02005"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOF95917.1"
                     /translation="MCGIAGMVAPDGEAADPTLVRAMAERLAHRGPDGEGEFFSGPVG
                     LAHRRLAIVGLSDEGRQPMANGDGSLQIVFDGEIYNFPEVREALVAAGHTFATATDTE
                     VILHAYEEWGHDCLSRFNGMWAFALWDGRRRELFCARDRLGVKPFYYTADAGSFLFAS
                     EIKALLVHAGRRPNDRMLLTFLAWGVADHTPETMYEGIVQLPPAHFVVVSEAGAGEPE
                     RYWDIAVNGAPRGADDEEAAQGVRSLLADAVRLRLQSDAPVGICLSGGIDSSTVTALA
                     NVLLQSGHPKSVGDRQKTFSVSFEDPRFDESRHIDTVVAATGVANRRTTPDTEGLWED
                     IGRLLYMQDEPFASLSIYAQYCVMRLASSEVKVVLDGQGADEQLAGYIAYQMPYIRGL
                     LRQGEVFAALREAAGSARRHTSFFSWAARQFAVRSERRGLLKGDAPEVLRYAGSLDEV
                     LAREITASNLPLLLHWGDRNAMAFSIEARVPFLDYRLVEYLAGLPLDQKIRSGVTKYV
                     LRRAIEGLVPDAIRCRMDKMGFVTPEEAWMKDELRPHILALFSSPEFVRRPYWDAERV
                     LRNYREFLEGKSPYSTEFWRIACTELWLRGFADR"
     gene            complement(4374..4820)
                     /locus_tag="PLV90_02010"
     CDS             complement(4374..4820)
                     /locus_tag="PLV90_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOF95918.1"
                     /translation="MDFERILARVAERGSDAIARELLREIENEYGRVPLIFERMAERP
                     EILISHLLYKGAVVETTRLDPKTIELISLAVGAALKCTHCVEYHMQAAKAKGATRDEI
                     LEAILIAGMLANSAVLADAYRVVNGSIPCASCDINGTGLDKTCLDE"
     gene            complement(4823..5764)
                     /locus_tag="PLV90_02015"
     CDS             complement(4823..5764)
                     /locus_tag="PLV90_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 7 protein"
                     /protein_id="HOF95919.1"
                     /translation="MTTLVPVTYKGGVYRHDEIMDLIDDLGGYIVQKHVMAQDVVLQS
                     FVPRDDIDLIRTVAKPLAGDVIEAPLVGTEIAVVSPSLEIHHLPHTACDIAEYLRRSG
                     AKTNMIGLARGFGKRIANLNDEERDVINEHDLAVYVLGNFEECIEHKFPALRRGIQVP
                     IVVTGAPDRETLARIVDPPVEGYVGGVGRIMHRFKRPEELAKLDELVDEVSRALDARR
                     DALARDPLSVFPPRLMAIVEEQIPEVTMLTHPTPVTAQMEGLRVKLPYDLYADDVRSL
                     DVTGGVTVGDIADVLPSRMRNYILIRIKPFSETRILV"
     gene            complement(5761..6345)
                     /locus_tag="PLV90_02020"
     CDS             complement(5761..6345)
                     /locus_tag="PLV90_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 17 protein"
                     /protein_id="HOF95920.1"
                     /translation="MPPLNYFVVESPDPVGREAYERITGDVLQDLDLIKVIDRLHIYV
                     DPKVPIFIAAGVLRHMPRSIRVGDFANVNPGEKLIVLDIGDETYLAPLLRELWAHYGK
                     ENVDQPDRFTIVLPAGVVGEEELEQMVVADPSETLHKDIIYALQYIAPEGFKVRRQYI
                     KDEKFYYVASEDTLPGDIVETMVVPIFAKMGVTL"
     gene            complement(6346..7584)
                     /locus_tag="PLV90_02025"
     CDS             complement(6346..7584)
                     /locus_tag="PLV90_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 15 protein"
                     /protein_id="HOF95921.1"
                     /translation="MSQKVRIAQLSCGPEYSGVQKEIYDAAGSVGAEVFFPDIALADV
                     ERAVDRFGLDVRSADLKLMIARGMALVDGKVDADGVFIGTCFRCAEAAIVRNELRRYI
                     PEHSRLPVVSYSFTERTTAGTLLTRMEALTTIARRRALLAREEQTGITMGVDSGSSTT
                     KAVVMKDNEIVGTGWHPTTEVIASADSVVADALAMAGVVPDDIQAIGTTGYGRFLIGK
                     HFGAELVQEELTVNSKGAVYLAGHQKGSATVIDIGGMDNKAISVIDGIPGTFTMGGIC
                     AGASGRFLEMTAKRLGVDITELGPLAMKGMGKNVPMNSYCIVFGTQSLVNALAAGSSR
                     EDVAAAACHSVAEQVFEQQLQEVDIKEPVIMVGGTSLIEGLVYAMGQLLQTEVVVPPY
                     SQYIGAVGSALLASGFVKGE"
     gene            complement(7581..8033)
                     /locus_tag="PLV90_02030"
     CDS             complement(7581..8033)
                     /locus_tag="PLV90_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 5 protein"
                     /protein_id="HOF95922.1"
                     /translation="MAKVFIYPATSLILSDLVARFGHKPLGAALGIRERIQTAGVDSP
                     PLQITPEEPKRGLKYAAVEVPSGVRGRMAIYGPLIEEAEAAVIVTDADFAFGCMGCAR
                     TDELILFSLRQSGIPILELKYPTTDDEGVRFVAAIKKFLAGLPNGGAA"
     gene            complement(8039..8494)
                     /locus_tag="PLV90_02035"
     CDS             complement(8039..8494)
                     /locus_tag="PLV90_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 6 protein"
                     /protein_id="HOF95923.1"
                     /translation="MAEYIPGYVGTVTKYVFVESPVMTPGELALRAYEASEGVLIKET
                     CFGLQVTGEPEAVDRLIARIRTFDPMHIFVKDRGFPPGDPRRCRANLGGARPGYLGHE
                     REFRILRYITRGLEEVERRGEEAETPEPAPEKRSGITIRRLQELIDEES"
     gene            complement(8495..10024)
                     /locus_tag="PLV90_02040"
     CDS             complement(8495..10024)
                     /locus_tag="PLV90_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012108012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 3 protein"
                     /protein_id="HOF95924.1"
                     /translation="MMEIYLDRDRIEVPEGARLGDILTGWDARCSVAVIRPALEEDAT
                     ESQEVRFATSAGDMVVEMADPALVSRLFAPGLAERLRLHWQDRYAAAFGPFETGIRPA
                     RAPGRYERGDVILGCGGYDPARSYLIFARMRHTADYGAPADGGVIGRVVTGRGLLDRI
                     AEGDRVVEIKRILRRADRSHAIVTRDPGLPLEDGMQVVSSIEAEVQGFTGGEIDTRAA
                     RSVEHFLLSVQSGRFRVDRSSSTHITDTHLVPTKVPPEFIGPRLEGSITVRTAGKSSG
                     ATYIYTQGVSASPAHTVVGRVVHGLELVRFSSDGDLLAIRVEPEQFDLVGLTLAEAEA
                     VAERRGIALTADAAGGDRMVVGQTPATTLEVLSAGAARVATEAAENVVSITLDEAAAP
                     RSVTIFREVTGLKHHAVGKMPLVFRFEDVSLFKPKIGKGVGIIPENTPTGEVPAFTLA
                     MTNDSRRGAGMVGIRETPNAEFGPTAEPLTGTNVIGKVLDAGSLAKMREGTTVYVREV
                     K"
     gene            complement(10032..11645)
                     /gene="atwA"
                     /locus_tag="PLV90_02045"
     CDS             complement(10032..11645)
                     /gene="atwA"
                     /locus_tag="PLV90_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl coenzyme M reductase system, component
                     A2"
                     /protein_id="HOF95925.1"
                     /translation="MTPLITVKDLCMEFNGSVVLKDIHFEVAEGETVGIIGRSGAGKT
                     VLMHLMRGVDQPPSRGAIIYHIAACGNCDYIGVPSEAGKPCPACGSELTPLDIDLWAE
                     ENAPMKRRIMRRTAIMFQRTFALYGDDRVIENVLRALDDVGYPSEKAVGRAADLIDQV
                     RLSHRMMHIARDLSGGEKQRVVLARQLAKNPFMLFADEPTGTLDPETATLVHRMLIES
                     AQKNDMAMVVTSHFSSVIEDVADRAILLENGEIKEIGDPGEVIRRFMEDYSDVERYQP
                     GEVGEKLLVARDVIKRYLSVDRGVVRAVNGVSFNVGEQEIFGIIGKSGAGKTTLSRII
                     SGILEPTSGEMNVRIGDDWVDMTKPGIEHRGRAKGYIGLLHQEYDLYPHRTVLDNLTD
                     AIGLEFPKELAMRKAIITLGMAGFTPEKSREILDRYPGQLSEGEKHRVALAQVLIREP
                     LLVVLDEPTGTMDPITKIDVKHSILHAREEMDETFIVVSHDMEFVRDICDRVALMRGG
                     KIIRLGPTEEVLAHLTDEERKVMGTGGAP"
     gene            complement(11693..12535)
                     /locus_tag="PLV90_02050"
     CDS             complement(11693..12535)
                     /locus_tag="PLV90_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOF95926.1"
                     /translation="MSVAVVFDSAGTLLRTYRVARDILREEMLIEVETTTITFGSDAR
                     VLVVLHLHSRDVIDAPPDQLLSTFFIERSVGFGVACSRRVIPAEEVANLLYADRCARI
                     SDMQACIREVWNCCKEESVVAMNSGVILNMDIPGIEFTVTTGGRPFDGAKETISELHR
                     MGVPAYIASGDRVTKLERMGDYLGIPRERVYGVATPSMKARIVEDLKEQYDKVVMVGD
                     AINDLHAFRKADLAVLTEQQSDRKPEVLYDCVDRVIRNVSEVPGIVAELLADTTAPGE
                     NATI"
     gene            12585..13514
                     /locus_tag="PLV90_02055"
     CDS             12585..13514
                     /locus_tag="PLV90_02055"
                     /EC_number="5.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0046033 - AMP metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphate isomerase"
                     /protein_id="HOF95927.1"
                     /translation="MILNGTAASIKSMEIRGAGRIARAAVEALADYARNLEVSDADAF
                     KREMQKAAEILTETRPTAVSLPNAVRYVMRALDSFESVEAAREAVLARAAEFIDHSEH
                     AVERIAEIGARHISDGDVLLTHCNSEAALGCILEAHRQEKEIEVYATEVRPRGQGLIT
                     IRALNDAGIRTNYIIDSAVRYFINDVDLVIVGADAIAVNGAVVNKIGTAQIAHAADEA
                     RTNVIVAAETYKFAPRTILGEKIAIEERDTGEVLPGTIAENLPFVRVRNPAFDVTPAE
                     YVDLIVTEMGAFPPELAYLVIRDYLGWKIEDLR"
     gene            complement(13580..13966)
                     /locus_tag="PLV90_02060"
     CDS             complement(13580..13966)
                     /locus_tag="PLV90_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOF95928.1"
                     /translation="MGLFKMTKTVLRNLAGGPATRCYPAVPARTSPLTRGHVAFDPAT
                     CRSCGICSRRCPCEAIRLDKEAKVWEIDRMRCIACGDCVEGCPFESLTMEQEYFPPVT
                     EHVVEHYTITYVKPEKPAKKPAEESA"
     gene            complement(13974..15053)
                     /locus_tag="PLV90_02065"
     CDS             complement(13974..15053)
                     /locus_tag="PLV90_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOF95929.1"
                     /translation="MSKRIVVPFGPQHPVLPEPIHLDLVLEDELVVDVVPSIGYIHRG
                     LEQLVLKREFTEYVYVAERICGICSFMHALCYSQGIEHIMNIEVPDRAKYLRTIWSEC
                     SRLHSHLLWLGLFADGMGFENLFMRSWQLRESVLDVLEDTTGGRVIQGTCKVGGVRRD
                     IGQEKLDEMHRTLDTFEENCRDLGDVFLNDGSVKYRLQGLSVIPRDDAYALGAAGPTA
                     RASGVAMDHRETGYAAYGDLGFKPVVETAGDCYARCAVRVRELYSSIDIIRRAIEGMP
                     EGPIDVKVKGTPDGEYFSRVEQPRGEVVHYLRGNGTKHLARARIRTPTLANIPILVKM
                     LPGIQLADVPVVVLSIDPCISCTER"
     gene            complement(15038..15424)
                     /locus_tag="PLV90_02070"
     CDS             complement(15038..15424)
                     /locus_tag="PLV90_02070"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOF95930.1"
                     /translation="MTMVHEEQTTMAVAREDLLNEVERLKTDGYRLVQIGCTDIGGAY
                     EINYSFDKGYQFKNLRLTVGPDEEVTSISGIYWGAFVYENEVHDLFGIPVTGINIDFK
                     GTFIRTATKYPFSVTRRGGDDPCQNA"
     gene            complement(15421..15888)
                     /locus_tag="PLV90_02075"
     CDS             complement(15421..15888)
                     /locus_tag="PLV90_02075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOF95931.1"
                     /translation="MAFLKRSPWILHYDASSCNGCDIEVLACLTPLYDIERFGIINTG
                     NPKHADILLITGGINEQNRPVVKNLYEQMPEPKVVVAIGACAATGGIFRECYNITGGV
                     DKVIPVDVYVPGCAARPEQIIDGVVTALGILEEKRMKMTGEATRPAAGVGEER"
     gene            complement(15894..16760)
                     /locus_tag="PLV90_02080"
     CDS             complement(15894..16760)
                     /locus_tag="PLV90_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOF95932.1"
                     /translation="MNWLWLIGAVLFLVLAPVAGGLIAGIDRKITARMQGRVGPPILQ
                     PFYDVGKLFEKERVVVTTAQNFYVLAYLVFIALSGAIFFAGGDLLLTVFAFTLAHVFL
                     VLGAYAVHSPYSHIGAERELIQVMAYEPMIILVVVGFYMVTGSFFVDAIAAATVPAIA
                     YLPGVFIGFAAILTIKLRKSPFDISTSHHAHQEIVKGITTEFSGSTLAQIEIAHWYEN
                     IFLLGLVYLFFAWNPVIAIIAVAVTYVAEIYIDNVTARVRWQAALKSGWLAAALGIAN
                     LAILSYMMIGGA"
     gene            complement(16757..18661)
                     /locus_tag="PLV90_02085"
     CDS             complement(16757..18661)
                     /locus_tag="PLV90_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOF95933.1"
                     /translation="MQTLLFLILFPILVACLLLFVKRTTSRYAVVALSALAIGGASVY
                     LLVRYASGGAVYFAASSEATDIVMVAIEMALALFIIAMGVKFRNYLAIALAVVQAALV
                     VYLETTFAGSLHAANNLFVDQFSIIMALIIGIIGSLIAVYGVRYMETYHHHHPEVRDR
                     QRMFFFVIFAFLAAMFGLVFSNNLMWVFFFWEVTTIASFVLIGYSETEEATTNAFRAL
                     VMNLLGGVAFVAALIFLAATGPTSGGVDLALVLASGDAAVILVPAVLIGFAGITKAAL
                     MPFSSWLLGAMVAPTPVSALLHSSTMVKAGVYILVRFAPVFAGTFAGLSVGLVGAVTF
                     VVASAIAISRSDAKSVLAYSTIANLGLIAACAGVGTYMLVWAAILLIIFHAVAKSLLF
                     LGTGAIEHRIGSRNIEDMEGLIVRMPKMAVMMFIGIAGMFLAPFGMLVSKWAAIEAFV
                     QAPFGLVFIAILAYGSAVTVFFWAKWMGKLVAVTRGTERIEKGFLEEPWVPLYITTAI
                     VILVVFLFPVISTALIEPYVFVIYGMTAHLAQANVAIMLLMISLLLILPISFSLFRKS
                     AKRLPVYMGGRPATPELQFAGSLGMTREAQTQNYYLTGYFGEARLYWPGAVVCIVLIL
                     ASWVFAGVAL"
     gene            complement(18915..19283)
                     /locus_tag="PLV90_02090"
     CDS             complement(18915..19283)
                     /locus_tag="PLV90_02090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOF95934.1"
                     /translation="MPCPFCNPAPEDIVARNDLCYALYDIHPVTTGHLLVIPFRHVAS
                     YFDTTDEEKMALARLIDECRAMTDHNLAPDGYNIGVNVGEAAGQNVMHVHVHFIPRYR
                     GDAAGEHEGGVRGVLPCRPW"
     gene            complement(19333..20511)
                     /locus_tag="PLV90_02095"
     CDS             complement(19333..20511)
                     /locus_tag="PLV90_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOF95935.1"
                     /translation="MQPAGRDATVAVARCDGYAPDEVDAAVRRAIDLVGGMERFVAPG
                     AKVLLKPNLLQGQAPERCVTTHPAVVAAVARLLAERGCRVVIADSPGAGIVYNEPNLR
                     RAYSRSGFAAVAEETDAVLNYDTGFRVIPFPEGEMVKQFPIIAPALEADAIVVVSKAK
                     THMWTRMTGAAKNLFGLVPGLEKPVFHFRFRDEYAFGSMLVDLNECMKPRLQIVDAVI
                     GMEGDGPQAGSPRKIGVILAGSDYAAVDTVLARLIGMDPLEIGTTRSAAARGLVDPSA
                     VRTVGDDPGEFVVPDFRKPSTYAGGRVGTWRRVVLAVVQRFGRTYAPRPGVIAPACIG
                     CGKCERICPVHAITVTEGRATVDLSRCIRCYCCHEICTEHAIALSRGFTGRLLARLLG
                     "
     gene            complement(20513..21289)
                     /locus_tag="PLV90_02100"
     CDS             complement(20513..21289)
                     /locus_tag="PLV90_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HOF95936.1"
                     /translation="MSLATIREKLRFGSFFRRRTPAIRGPASRDREAEMRALLARPPF
                     AITDIADAALPLYDRALTHRSYANGSEYPIAAADDNERLEFLGNYVLDFVIADHLYGE
                     YDLPPGEMNRRLQVTRNTKLADIVHRQGLGIDGLIRRRGQAPTDSIIADAFEALIGAI
                     YVDRGIGAAREVVLAIFEDEIAGCDTRRNYRGRLQELVARKGLGGIDYIYRRTGPGSS
                     PVWVARVIVGGTPLGRGEAPTKQGAAMLAAREALGRLGEE"
     gene            21400..22146
                     /locus_tag="PLV90_02105"
     CDS             21400..22146
                     /locus_tag="PLV90_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95937.1"
                     /translation="MQSAAVGGGGSVYLDIEFGYVYGTSRAVMMPVEIGAVIHHPEDD
                     SVRYAGEQFRYDIDVEVWKKVTDPCGRTVGVATTVANMGRGRYGGAYDHYFRLPGDRV
                     PAAEETAGKAFADLRVFMESLLTDDITEIVVFAADMERRAFRTADVPLDGRRLVDLQR
                     EIRRRLGMKQVLSLDRLARLIDFSAENGAVASTHFRYPVPPGYRHLLDVHRGMGDAVR
                     MFLLAREFREKLPELEKRVRALEDTCGGEE"
     gene            complement(22109..23275)
                     /locus_tag="PLV90_02110"
     CDS             complement(22109..23275)
                     /locus_tag="PLV90_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOF95938.1"
                     /translation="MMRSTFPCPDRTTRVPVAASAGRVTARPVHSPMTVPATDTAARD
                     GFAVISAETRGAGDATPVRLRNPHRVNTGNAIPPGYDAVVMIEDIAGEDGTWSTRKAA
                     APGEHIHPAGSEIEQGERILPAGHRIRPCDIGALLTYGIAEIDVPAVKVGLIPTGNEL
                     VPAGGHPGAGEVIESNTAVAAAWLCEAGASPTRYGIVADDPAALRQAIAKAVRENDLV
                     LVSAGSSTGTRDFTAGVIGELGEVLVHGIAMKPGRPAIVGRVEDKPVIGMPGYPIAAL
                     TTIRELALPLLAEWGFCAPPAERLRARLCESVLADPEFDEFILLTVYRADDGYTAVPL
                     PPHSRTQMAMVRANAYLHVPAGSGAIPKGAEVDVILTGPCAPVTPRPRRYPQEP"
     gene            complement(23334..24134)
                     /locus_tag="PLV90_02115"
     CDS             complement(23334..24134)
                     /locus_tag="PLV90_02115"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HOF95939.1"
                     /translation="MKTVTITLVNPPELYLEGQNITPDNVAGKTAAEIATLDVWEGKQ
                     KSPLGKYFTVSGNGGATAADTKIVFRGDCTRVKRIGQQMTAGEIVVEGNADMYIGGWM
                     KGGKIHVKGNVDSFCGIGMEGGELIVDGNAGHHLGSSPRGEWRGMQGGLIRVAGDAGN
                     DTATFINGGTIIIGGNADIHVSTHGEGGTVIIKGNAKGRVGGQMVKGDIYVFGTIENP
                     LPGFQRVGEVEQEVAGEKACFIHHVGDLGERHPIAKGKAVYANLYTRI"
     gene            complement(24148..>25620)
                     /locus_tag="PLV90_02120"
     CDS             complement(24148..>25620)
                     /locus_tag="PLV90_02120"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HOF95940.1"
                     /translation="LFKSPLKRGNRMEMGFSVPSTFKTGYDYARMGYVFVNEAAMPPL
                     HSPHVHEEIRDTPIIDNAAMPVFGNNWFTLDYLKNNEIENNAAYTAWLLRATRGFAIK
                     VVNPGGTEAWGWGLNCENIHDTVPYFDITPAKIITGLIETNEFLGLPHSMHLHTNNLG
                     NPGNYTDTLDSLRLAEGYTAKNNFGRDQVMHVTHVQFCSYGGDSWKNIDSQAKTIMDY
                     VNAHDNITIDMGQVTLDETTTMTADGPFEHHLRGLNNLKWANTDVELETAAGVVPYIY
                     SPNVKVSTIQWAVGLELALYSNDLMRTFITTDHPNAGPFTRYPRVIKWLMSRAARDAT
                     MKTFKWLDRTIESTTIDSIDRELTLYEIAQMTRAGPAKALGISNTYGGLAPGMDGDIA
                     VYALNPENMPSDPDRIEEAFSRCAYLVKDGVVTARGGEVVTEPAKRTIWVDVKVPENA
                     QVQRDIYEHFLRHYSVGVKNYQLFDEHLHNPRVIEVDTTQ"
BASE COUNT         4312 a         8343 c         8256 g         4709 t
ORIGIN      
        1 gaggtctgct acacccggct cgtccgggag gagaatacga gggcgggggt gcggtaagcg
       61 gccggagcgg cgccggggtc gcagggtatc cggcccgtac ctgaacaggg caccccatca
      121 tcgccggtgc gggacaaagg gcgaaacccc gtcgccacct ccatcacgtc cggtgtgtga
      181 aacgagaggg ccggcgtttc ctggtggggt atcccgagtt cggcgtggat gatggtgttc
      241 gcggggttcc tcgcacccac gcaccccccc gccgaccatc cggctctcca gcgccctttc
      301 cggccccccc ctcactccag cgtcaccggg agcccggcga ccgcccaggc gctcataccg
      361 ccctcgacct cgtagacctc gcgaaacccg gcctcccgca tcatctcccg gacgcccgag
      421 ctccgcccgc cccgctggca gtagatgacg taggtcccgt tccggtcgag atccgtgagg
      481 cgtccctcaa actcccggga gtcgatgttg atcgcgcccg gcacgtgccc ggcggcgaac
      541 tcgtccggcc tccggagatc gatgacggca aacgccgggt cctgccccct ctcatcgatc
      601 agggcggacg ccccggcggg cggcacggtc cggatgatca cgccggactc agccgggccg
      661 gtgcacccgg cgatgagcac gaccgcgacg acaagcacga gcagaggata cacctcgatc
      721 atacatgcat gtcgtgatcg ccgggataaa ggagttgcgg tcccccgggg cagctcaggc
      781 ggtatcgtcg aacccgggcg ccgcatcgtc catgcccatc accgcacaga gggtatccgg
      841 cgtatccagg gccacgacct ccccgccgtc gaccacggcg acccggtggc agatcgccct
      901 cgcctcctcc aggctctcgg tcgtcaggac gatcgtcggc ctccggtccc ggttcaaccg
      961 ctggagcgct tcccagatcc cccggcggcc ggccccgtcc agccccttcg tcggctcggc
     1021 aagaaagagg acggacgggt ggataagata cgcccgggcg atctcaagcg accgcgccat
     1081 ggcaggagga aaggtctcca ccgtggcatc caggctcccg gagaggttga agagcctggc
     1141 gatctcggcg atccgcctcc gccgcgcccc ctcgccgagg ccgtgcatcc gtgcatggaa
     1201 gtccaggttc tccctcgcgg tcagggcggg gtcgaggatg ggctccggaa agacgactcc
     1261 catgctccgc cgcacgtccc cgaggttccc gaggatcgca agcggcgacc gctcggcaag
     1321 cgccgggacg ggaaagatcg tcgtcatgag gatggcggtc aggatccgcc ggcccttgcc
     1381 gcaggtgcag aggataccga aaacctctcc gctcttcacg tcgaacggta tagggcctcg
     1441 cgcctgaagg cgctccaggc accggatcag atcctcaacg gtaacgacag tcatgcgcct
     1501 ccacagggat cccggccgca gatcccccgg aattccggga cggcggcttt acggcgaaat
     1561 atcgcccggg gattcgatat tgagtttccg ttacccgatt cggggaagga attgctgaat
     1621 aatcatatct taattgtcgc tatatgaaca tatctattta gaaatggata taaaatcaac
     1681 cgtttaagat tttcgtattg tctcaggtgc cctttttggc gtaaacctgc tcacccacat
     1741 cttcaggcag gaagcggatc cgcaccgcag acctccgggg gccggatcaa agtatttatc
     1801 gtgcgggacc aaccggagta cccggcccat gacgaccgat atatccacga tagcgatcct
     1861 cccggtaccc ttcagccccg accaggccct gatcgctctc gccgtcctca taccggtcct
     1921 tctcctgctc atcctgctct tcatcagcgc aggcgtcttt gagtcgatcg ggttccggtt
     1981 ctaccaggcg gtgctggtga ccgccggcgc cctcttcggg agcctggtca acataccgct
     2041 catccccgtc ggcgaggcca tcatagcggt caacgtgggc ggcgccgtca tccctctctt
     2101 cgtgaccctc gatctcgtcg tcaggggccg ggtctcgcgc cccaaaaccc tcgccgccat
     2161 cgccgccgtc acgctcgtcg cctacatgct cgcaacaccc gtccccggca ccgggatcac
     2221 catgccgttc tacgtcgccc ccctcgccgg cgccgccgca ggcctcatcc tcgccggggg
     2281 ttgccatgcc gcccccgggc ttgcctacgc gggcggggcc atgggcaccc tcatcggcgc
     2341 agacatactc aacctcgcaa accctgcggt ctttgcagcg ctcaccggag gaaaagcggc
     2401 ggtcctctcc atcgggggcg cgggtatatt cgacggcatc ttcgtcaccg gcatcttcgc
     2461 ggtcctgctc gccgcctacg cggggagaca gctgcgaaag tcggcaggcg tctgcccccg
     2521 ggaggggaga gagtgaacac ccccgcacaa aagaatcgct accggtcggc aaacccgcgg
     2581 agccagagct ccgtgcaggc tatccgccag aactcggtgg agtacgggct cttcccctcg
     2641 aggaactccc ggtagttccg gagcacccgc tccgcgtccc agtagggccg tcttacaaac
     2701 tccggcgacg agaagagggc aagaatatgg gggcggagtt catccttcat ccaggcctcc
     2761 tcgggagtga caaaccccat cttgtccatc cggcaccgga tagcgtcggg caccagcccc
     2821 tcgatcgccc gccggaggac gtacttcgtg acgccggagc ggatcttctg gtcgaggggg
     2881 aggcctgcga ggtactccac cagccggtag tcgaggaacg gcacccgcgc ctcgatcgag
     2941 aacgccatag cgttccggtc cccccagtgg agcaggagcg gaaggttcga ggccgttatc
     3001 tcccgggcaa gcacctcgtc aagcgatccc gcgtaccgga ggacctccgg cgcatccccc
     3061 ttgagaagcc cccgccgctc cgaccggacg gcgaactggc gggccgccca cgagaagaac
     3121 gacgtatgac gccgcgcgct cccggcagcc tcccggagag ccgcgaacac ctccccctgc
     3181 cggaggagcc cgcggatata cggcatctgg taggcgatat acccggcaag ctgctcgtcg
     3241 gccccctgcc cgtcgaggac gaccttcacc tcgctacttg ccagccgcat aacacagtac
     3301 tgggcgtata tcgagagcga tgcaaacggc tcgtcctgca tataaaggag cctcccgata
     3361 tcctcccaga gcccctccgt gtcgggggtg gtccggcggt tcgcgacccc cgtcgccgca
     3421 accaccgtat cgatatgccg gctctcgtcg aaccggggat cctcgaaact gaccgagaac
     3481 gtcttctgcc ggtcgccgac gctcttcgga tgcccgctct gcaggaggac gttggccagg
     3541 gcggtgaccg tcgacgaatc gatacccccc gaaagacaga tcccgaccgg ggcgtcgctt
     3601 tggagccgga gccggaccgc gtcggcgagg agcgatcgaa ccccctgcgc ggcctcctcg
     3661 tcatcggccc cccgcggggc accgttcacc gcgatatccc agtagcgctc cggctccccg
     3721 gcgcccgcct ccgacaccac gacgaagtgg gccggcggga gctgaacgat cccctcgtac
     3781 atcgtctccg gcgtatggtc cgcgaccccc caggcgagga acgtaaggag catgcggtcg
     3841 tttggtctcc ggccggcatg cacgaggagc gccttgatct ccgaggcaaa gagaaacgat
     3901 ccggcatccg ccgtgtagta gaacggcttg acgcccaggc ggtcacgggc gcagaagagc
     3961 tcgcggcgcc ggccatccca gagggcgaac gcccacatgc cgttgaaccg ggagagacag
     4021 tcatgccccc actcctcgta ggcgtgcagg atcacctccg tatcggtcgc tgtggcaaag
     4081 gtgtggccgg cggcgacgag cgcctcgcgg acctcgggaa aattatagat ctcgccgtcg
     4141 aagacgatct ggagcgaccc gtccccgttt gccatcggct gccgcccctc atcggagaga
     4201 ccgacgatcg caaggcgccg gtgtgccagc cccaccgggc cggagaagaa ctccccttcg
     4261 ccgtcagggc cgcggtgggc gagccgttcc gccatggccc ggacgagcgt cggatccgcc
     4321 gcttccccgt ccggggcgac cattcctgct atcccgcaca tgtcgggccc ccgtcactcg
     4381 tccaggcagg tcttatccag gccggtgccg ttgatgtcgc aggacgcaca gggaatcgat
     4441 ccgttcacca cccggtaggc atccgcaagg accgcggagt tcgcgagcat gccggcgatc
     4501 aggatcgctt ccaggatctc gtcccgggtc gcgccctttg ccttagccgc ctgcatgtga
     4561 tactcgacgc aatgggtgca cttgagcgcg gcgccgaccg caaggctgat cagctcgatc
     4621 gtcttcgggt caagccgggt ggtctcgacc acggcgccct tgtagagcag gtgcgatatc
     4681 aggatctcgg gccgctccgc catacgctcg aatataaggg ggaccctgcc gtactcgttc
     4741 tcgatctccc ggaggagctc gcgtgcgata gcatccgatc cccgctctgc aaccctcgcc
     4801 agtatcctct caaaatccat cgctacacca atatcctggt ctccgagaag ggtttgatcc
     4861 gtatcaatat gtaattgcgc atccgcgacg gcagaacgtc ggcgatatcc ccgacggtga
     4921 ccccgccggt gacatcgagc gaccgcacgt cgtcggcgta gaggtcgtag gggagcttca
     4981 cccggagccc ctccatctgg gcggtcaccg gcgtcgggtg cgtcagcatg gtgacctcgg
     5041 ggatctgctc ttcaacgatc gccatcaggc ggggcgggaa gacggagagc gggtcccgtg
     5101 caagggcgtc ccgccgggca tccagggcgc gggagacctc gtccaccagc tcgtcgagtt
     5161 tcgcgagctc ctcggggcgc ttgaaccggt gcatgatcct ccccacaccg ccgacgtagc
     5221 cctcgacggg cggatcgacg atccgggcca gggtctcgcg gtccggtgct ccggtcacga
     5281 cgatcggcac ctggatcccc cgccggagcg ccgggaactt atgctcgatg cactcctcga
     5341 agttcccgag cacgtagacg gcaaggtcgt gctcgttgat gacgtcccgc tcctcgtcgt
     5401 ttaagttcgc tatccgcttc ccaaatcccc gggcaagccc gatcatgttc gtcttcgcgc
     5461 ccgagcgccg gaggtattcg gctatatcgc aggcggtgtg ggggagatgg tggatctcaa
     5521 ggctcgggct cacgacggcg atctccgtcc ccacgagagg cgcctcgatc acatcgccgg
     5581 caagcggttt tgcaaccgtc cgtatcaggt cgatgtcgtc tcgcggcacg aacgactgga
     5641 gcacgacgtc ctgggccatg acgtgcttct gcacgatgta gcccccgagg tcgtcgatca
     5701 ggtccatgat ctcgtcgtgc cggtagacac cgcccttgta ggtcaccggc accagtgtcg
     5761 tcacagcgtc acccccatct ttgcaaatat cggtacaacc atcgtctcca cgatgtcgcc
     5821 cgggagggta tcctcgcttg ccacatagta aaacttctca tccttgatgt actggcggcg
     5881 gaccttgaac ccctcggggg cgatgtactg gagggcgtag atgatgtctt tgtggagcgt
     5941 ctcgctcggg tcggcgacga ccatctgctc gagctcctcc tccccgacca cccccgccgg
     6001 gaggacgatg gtgaaccggt cgggctggtc gacgttctct ttcccgtagt gggcccagag
     6061 ctctctgagc agcggcgcaa ggtaggtctc gtcaccgata tcgaggacga tcagcttctc
     6121 gccggggttg acgttggcaa agtcgccgac ccggatggac cgcggcatgt ggcggagcac
     6181 cccggccgcg atgaagatcg gcaccttcgg gtcgacgtat atgtggaggc ggtcgatcac
     6241 cttgatgaga tcgaggtcct ggaggacgtc gccggttatc cgctcgtagg cctctctccc
     6301 gaccggatcg ggcgactcga cgacgaagta gttcaacggg ggcatctact ctcctttgac
     6361 gaatccggat gccagcaggg ccgacccgac cgcgccgatg tactgcgagt agggcgggac
     6421 gacgacctcc gtctgcagga gctggcccat cgcgtagacc agcccctcga tcagggaggt
     6481 gccgcccacc atgatcaccg gctccttgat gtcgacctcc tggagctgct gttcaaagac
     6541 ctgttcggcg acgctgtggc aggccgcagc cgccacatcc tcccgcgacg acccggcggc
     6601 aagcgcgttc accaggctct gcgtcccaaa gacgatgcag taactgttca tcgggacgtt
     6661 tttgcccatg cccttcatgg cgagggggcc gagttcggtg atgtccacgc cgagacgctt
     6721 tgcggtcatc tcgaggaacc tgccgctcgc gccggcacat atgccgccca tcgtgaacgt
     6781 cccgggtatg ccgtcgatga cgctgatcgc cttgttgtcc atgccgccga tatcgatgac
     6841 ggtcgcagac cccttctggt ggccggcaag gtagacggcg cccttcgagt tcaccgtcag
     6901 ctcttcctgg acgagttcgg cgccgaagtg tttcccgatg aggaaccggc cgtacccggt
     6961 cgtccctatc gcctggatat cgtcggggac aaccccggcc atcgcaaggg cgtcggcaac
     7021 cacgctatcg gcgctcgcga tcacctcggt cgtcgggtgc cacccggtgc cgacgatctc
     7081 gttatctttc atcacgacag ccttcgtcgt gctcgacccc gagtcgacgc ccatcgtgat
     7141 accggtctgc tcctcgcggg caaggagcgc ccggcggcgg gcgatggtgg tcagggcctc
     7201 catccgggtg aggagggtgc ctgcggtcgt ccgctcggtg aaggagtaac tcaccaccgg
     7261 gagccgcgag tgttcgggta tgtagcggcg gagctcgttt cggacgatag ccgcctcggc
     7321 gcaccggaag caggtgccta tgaagacgcc gtcggcgtcc accttcccgt ccacgagcgc
     7381 catgccccgg gctatcatca gtttcaagtc ggcgctccgg acatcgaggc cgaaccggtc
     7441 gaccgcccgc tcgacgtcgg caagagcgat gtcggggaag aagacctccg cgcccaccga
     7501 tcctgcagcg tcgtatatct ctttctggac gccgctgtac tcgggcccgc aggagagctg
     7561 ggcgatccgc accttctggc tcatgctgca cccccgttcg ggaggccggc gaggaacttc
     7621 ttgatggcgg caacgaaccg gacgccttcg tcgtcggtcg tgggatactt cagttcgagg
     7681 atcggtatgc cgctctggcg cagggaaaag aggatcagct cgtcggtcct ggcgcacccc
     7741 atgcacccga acgcgaagtc cgcgtcggtg acgatgaccg cggcctccgc ctcctcgatg
     7801 agcgggccgt atatcgccat ccgtccccgg acaccggacg gcacctcgac ggccgcgtac
     7861 ttcaggccgc gtttcggttc ttcgggcgtg atctgcaggg gcggggagtc aaccccggcg
     7921 gtctgtatcc gctcccggat accaagggct gcaccgagcg gtttgtggcc gaaccgggcc
     7981 acgaggtcgg agaggataag gctcgttgca ggatatatga acacctttgc cattctcttc
     8041 aggactcctc gtctatgagt tcttgtagtc gcctgatagt tattccggat ctcttctcgg
     8101 gggccggttc cggcgtctcc gcctcttctc cccgccgctc gacctcttcg agaccccggg
     8161 tgatgtagcg caggatgcgg aactcgcgct cgtggccgag gtagcccggc cgcgccccgc
     8221 ccaggtttgc ccggcaccgc cgcgggtcgc cgggcgggaa cccgcggtct ttgacgaata
     8281 tgtgcatggg gtcaaacgtc cgtatccggg ctatgagccg gtcgacggcc tccggctcgc
     8341 ccgtcacctg caggccaaaa caggtctcct tgatgaggac gccctcggac gcctcgtatg
     8401 ccctgagggc aagctcgccc ggggtcatca ccggcgactc gacgaagacg tacttcgtga
     8461 ccgtgccgac gtaccccggt atatactcag ccactcactt cacctctctc acgtagaccg
     8521 tcgttccctc gcgcatttta gcaaggctcc ccgcatcgag caccttcccg atgacgttcg
     8581 tgccggtgag gggctcggcg gtcgggccga actcggcgtt cggcgtctcc cgtatgccga
     8641 ccatgccggc cccccgccgc gaatcgttcg tcatcgcaag ggtgaacgcc gggacctccc
     8701 cggtgggggt gttctccggt atgatcccga cgcccttccc gatcttcggc ttgaaaagag
     8761 agacgtcctc gaaccggaag acgagcggca tcttcccgac ggcgtggtgc ttgagcccgg
     8821 tcacctcgcg aaagatcgtg accgaccggg gcgcggccgc ctcgtcgagg gtgatgctga
     8881 cgacgttctc ggccgcctcc gttgcgaccc gggccgcacc ggcagaaagc acctcaagcg
     8941 tcgttgccgg cgtctggccg accaccatcc ggtctccgcc ggccgcatcg gcggtgagcg
     9001 cgatcccccg ccgctcggcg accgcctccg cctccgcaag ggtcaggccg accaggtcga
     9061 actgttcggg ctcgacgcgg atggcaagga ggtcgccgtc gctggaaaac cgcacgagtt
     9121 caaggccgtg gaccacccgc ccgacgacgg tgtgggccgg gcttgccgat acgccctgcg
     9181 tgtagatgta ggtcgctccc gaggatttcc cggcggtcct gaccgtgatg gacccctcga
     9241 gccggggtcc gatgaactcc ggcgggacct tcgtcgggac caggtgggtg tcggtgatat
     9301 gggtgctgct tgaacggtcc acccggaacc ggccgctctg caccgagagc aggaagtgtt
     9361 cgacgctccg ggcggcccgg gtatcgatct cgcctcccgt aaacccctgc acctccgcct
     9421 cgatagagga gacgacctgc atcccgtcct cgagcggcag cccggggtca cgggtgacga
     9481 tcgcgtgcga ccgatctgcg cgcctgagta tccgtttgat ctcgacgacc cggtcgcctt
     9541 cggcgatgcg gtcgagcagc ccccggccgg tgacgaccct gccgatgacg ccgccgtcgg
     9601 caggggcgcc gtagtcggcg gtatgccgca tgcgtgcgaa tatcaggtag gaccgggcag
     9661 ggtcatagcc gccgcacccg agtatgacgt cgccccgctc gtaccgcccc ggcgcccgcg
     9721 cggggcggat gccggtctca aacggtccga aggcggcggc gtagcggtcc tgccagtgca
     9781 ggcggagccg ttcggcaagc cccggcgcaa agagccttga aaccagggcc ggatccgcca
     9841 tctcgacgac catatccccc gccgacgtgg cgaacctgac ctcctgcgac tcggtcgcgt
     9901 cctcctcgag cgccgggcgg atgacggcga cgctgcaccg ggcgtcccat ccggtgagga
     9961 tatccccgag ccgggcgccc tccggaacct cgatacgatc tctgtccagg tagatctcca
    10021 tcattcgtgc ctcagggggc gccgccggtc cccatcacct ttcgttcctc gtcggtgagg
    10081 tgcgcgagca cctcttccgt cggccccagc ctgatgatct tgccgccccg catcagcgcc
    10141 acccggtcgc atatgtcccg cacgaactcc atgtcgtgcg agaccacgat gaacgtctcg
    10201 tccatctcct cgcgggcgtg gaggatagag tgtttcacgt cgatcttcgt gatggggtcc
    10261 atcgtgccgg tcggctcgtc gaggacgacg agcaggggct cgcgaatgag aacctgggcg
    10321 agcgccaccc ggtgtttctc tccttccgag agctgccccg ggtaacggtc caggatctct
    10381 ctgctcttct cgggggtgaa cccggccata ccgagggtga tgatcgcctt tcgcatggca
    10441 agttcttttg ggaactcgag gcctatggcg tcggtgaggt tgtcgagcac cgttctgtgc
    10501 gggtagaggt cgtactcctg gtggagaagc ccgatgtagc ctttcgcccg tccccggtgt
    10561 tcgatgccgg gtttggtcat atcgacccag tcgtcgccta tccggacgtt catctcgccg
    10621 ctcgtgggtt caaggatccc ggatatgatc cgcgagaggg tcgtcttgcc cgcaccgctc
    10681 ttgccgatga tgccgaagat ctcctgctcg ccgacgttga aggagacgcc gttgaccgcc
    10741 ctgaccacgc cccggtcgac cgagaggtag cgcttgatca cgtcgcgggc gaccaggagc
    10801 ttctcgccga cctccccggg ctggtagcgc tcgacgtcgc tgtagtcctc catgaaccgg
    10861 cggatgacct cccccgggtc cccgatctcc ttgatctcgc cgttctcaag caggattgcc
    10921 cggtccgcga catcctcgat gacgcttgag aagtgcgacg tgaccaccat cgccatatcg
    10981 ttcttctgcg cgctctctat gagcatccgg tggacgagcg tggcggtctc cgggtcgagc
    11041 gtcccggtcg gttcgtccgc aaagagcatg aacgggttct ttgcaagctg ccgggcaagc
    11101 actacccgct gcttctcgcc gccggagagg tcgcgggcga tatgcatcat ccggtgggag
    11161 agcctgacct gatcgatgag atcggccgcc ctgccgaccg ccttctccga ggggtaaccg
    11221 acgtcgtcga gcgcccgcag gacgttctcg atcacccggt cgtcgccgta gagggcaaac
    11281 gtcctctgga acatgatggc ggtccttcgc atgatccgcc gtttcatggg cgcattctct
    11341 tcggcccaga gatcgatatc caggggcgtg agttcgcttc cgcaggcagg acatggtttc
    11401 cccgcctcgc tcgggacgcc gatgtagtcg cagttcccgc aggcggcgat atggtagatg
    11461 atcgcacccc gtgacggggg ttgatctaca ccccgcatga gatgcataag aacggttttg
    11521 ccggccccgc ttcttcctat gatgccgact gtttcacctt ctgcaacctc gaagtgtatg
    11581 tctttgagta caacgctccc gttgaactcc atgcagaggt ctttcaccgt gatgagtggg
    11641 gtcatcttga gttcctcgtt aaagttatct tctcgtggta tcatattagg gtttatattg
    11701 ttgcattttc cccgggcgcc gtggtgtccg cgagaagttc cgccacgata ccgggcacct
    11761 cgctgacgtt ccggatcacc cgatcgacac agtcatataa aacctcgggc ttccgatcgg
    11821 actgctgctc ggtgagcacc gcgagatccg ccttccggaa ggcgtggagg tcgttgatgg
    11881 catcccctac catgacgact ttgtcatact gttctttaag gtcttccacg atccgggcct
    11941 tcatcgaggg ggtggcgaca ccgtagaccc gttcccgggg gataccgagg tagtcgccca
    12001 tccgctcgag tttcgtgaca cggtcccccg acgcgatgta ggcggggacg cccattcggt
    12061 gcagttcgct gatggtctct ttcgcgccgt caaacggtct gccgccggtc gtcaccgtga
    12121 actcgatccc gggtatatcc atgttcagga tcacgccgct gttcatggcg acgacagact
    12181 cttctttgca gcagttccag acctccctga tgcaggcctg catatcgctg atgcgggcgc
    12241 atcggtcggc gtagaggagg tttgcgacct cttcggcggg gatgacccgc cgggagcagg
    12301 ccacgccgaa accgaccgac cgctcgatga agaacgtcga gaggagctgg tcgggaggcg
    12361 catcgatgac gtcgcgggag tggaggtgga ggacgacgag cacccgtgca tcggacccga
    12421 aggtgatggt cgtggtctcg acctcgatga gcatctcttc gcggaggata tcgcgcgcta
    12481 cacggtaagt ccggagaagt gtgcctgcac tatcaaaaac aacggctacc gacatctacc
    12541 ctcacgtcaa tacatctatg tggcgctcgc ttgaatgaat gattatgata ctgaatggga
    12601 cggcggcaag cataaagagt atggagattc ggggagccgg caggatcgcc cgggcggcgg
    12661 tggaggcgct ggccgattac gctcgaaatc tggaagtttc cgacgccgat gccttcaagc
    12721 gggagatgca gaaggcggca gagatcctca cggagacccg cccgaccgcc gtctcgctcc
    12781 cgaacgcggt gcgctacgtg atgcgggccc tcgactcgtt tgagtccgtg gaggccgcac
    12841 gggaagcggt cctcgcgcgg gcggcggagt tcatcgatca ctcggagcac gcggtcgagc
    12901 ggatagccga gatcggtgct cgtcatatat ccgacggcga cgtcctcctg acccactgca
    12961 actccgaggc ggctctcggg tgtatccttg aagcgcaccg gcaggaaaaa gagatcgagg
    13021 tctacgcgac ggaggtgcgg ccgcggggcc aggggctcat caccatcagg gcgctgaacg
    13081 atgccggcat acggacgaac tacatcatcg actcggcggt ccgctacttc atcaacgacg
    13141 tcgacctggt catcgtcggc gccgatgcca tagcggtgaa cggagcggtc gtgaacaaga
    13201 tcgggaccgc ccagatcgcg cacgccgcag acgaggcaag gacgaacgtc atcgtcgcgg
    13261 cggagaccta caagttcgcg ccgcggacca tactcggcga gaagatagcg atcgaggagc
    13321 gcgacaccgg cgaggtgctg cccggaacta tagcagagaa ccttcctttc gtccgggtcc
    13381 gaaacccggc cttcgacgtg acgcccgcgg agtacgtcga cctgatcgtc accgagatgg
    13441 gggcgttccc gccggagctt gcgtatctgg tcatacggga ttacctcggg tggaagatcg
    13501 aagacctgcg gtgaaggcga tagttcctcc cgattgccga ataaaaaggt ctttgccggg
    13561 ggtccgggcc ccggctctct tacgcgctct cttcggccgg cttctttgcc ggtttctcgg
    13621 gtttcacgta ggtgatggtg tagtgctcca ccacgtgctc cgtcaccggc gggaagtact
    13681 cctgctccat cgtgaggctc tcaaacgggc agccctcgac gcagtcgccg caggcgatgc
    13741 agcgcatacg gtcgatctcc cagaccttcg cctccttgtc gaggcggatc gcctcgcacg
    13801 ggcaccgccg cgagcagatg ccgcaggagc ggcacgtggc cgggtcgaac gcgacgtgcc
    13861 cccgggtgag ggggctcgtc cgtgccggga ccgccgggta gcaccgggtg gccggtcctc
    13921 ccgcaaggtt ccggagaacc gtctttgtca tcttaaaaag tcccatgccg ccgtcacctc
    13981 tccgtgcagc tgatgcaggg atcgatcgag aggacgacga ccgggacatc ggcaagctgg
    14041 atgccgggga gcatcttcac gagtatgggt atgtttgcaa gcgtcggcgt cctgatgcgg
    14101 gcgcgggcga ggtgcttggt gccgttgccg cgcaggtagt ggacgacctc gccccgcggc
    14161 tgctccacgc gggagaagta ctcgccgtcc ggcgttccct tcaccttcac gtcgatgggt
    14221 ccttcgggca tcccctcgat cgccctccgg atgatgtcga tcgacgagta gagctccctg
    14281 accctgacgg cacaccgggc gtagcagtca ccggccgtct cgacgaccgg tttgaaacca
    14341 aggtccccgt aggccgcgta tccggtctcg cggtggtcca tggcgactcc gcttgctctc
    14401 gcggtcggcc ctgccgcccc gagggcgtaa gcgtcgtccc ggggtatgac cgagagcccc
    14461 tggaggcggt acttcaccga tccgtcgttt aagaagacgt cgccgaggtc ccggcagttc
    14521 tcctcgaagg tgtcgagcgt ccggtgcatc tcgtcgagtt tctcctgccc gatgtcgcgc
    14581 ctgaccccgc cgaccttaca ggtcccctgg atgacccggc cgccggtggt gtcctcgagg
    14641 acgtcgagca cgctctcgcg gagctgccag gagcgcatga agaggttctc aaaacccatg
    14701 ccgtccgcaa agaggccgag ccagaggagg tgggagtgga gacgcgaaca ctccgaccag
    14761 atcgtccgga gatacttcgc ccggtcggga acctcgatgt tcatgatatg ctcgatgccc
    14821 tgggagtagc agagagcgtg catgaaactg catatgccgc agatccgctc cgccacgtag
    14881 acgtactcgg tgaactcgcg cttcagcacg agctgttcaa gcccccggtg gatgtagccg
    14941 atggagggaa ccacgtccac caccagctcg tcctctaaga cgaggtcgag gtggatcggt
    15001 tccggcagca ccgggtgctg cggcccgaac ggcacgacta tgcgttttga cacgggtcgt
    15061 cacctcctcg ccgcgtgacg ctgaacggat acttcgttgc cgtcctgatg aacgtcccct
    15121 taaagtcgat gttgatcccg gtcaccggta tgccgaagag gtcgtgcacc tcgttctcgt
    15181 agacgaacgc tccccagtag ataccggata tgctcgttac ctcctcatcc ggcccgaccg
    15241 tcaggcggag gttcttgaac tggtagcctt tgtcgaacga gtagttgatc tcgtaagccc
    15301 cgccgatgtc cgtgcatcct atctggacga ggcggtagcc gtcggtcttg agtctttcga
    15361 cctcgttcag gaggtcttcc cgcgcgacgg ccatcgtcgt ctgctcttcg tgcaccatcg
    15421 tcatctctcc tcacccaccc ctgcggcagg tcgcgttgct tcccctgtca tcttcatccg
    15481 tttctcctcg aggatcccga gcgccgtaac gacgccgtcg atgatctgct ccggccgggc
    15541 cgcgcatccc gggacataga cgtcgacggg gataactttg tcgacgcccc ccgtgatgtt
    15601 gtagcactcc cggaatatgc cgccggtcgc ggcgcaggcg ccgatagcga cgacgacttt
    15661 gggctccggc atctgctcgt agaggttctt gaccaccggc cggttctgtt cgttgatccc
    15721 gccggtgatc agcaggatgt ccgcatgctt cgggttgccg gtgttgatga tgccgaaccg
    15781 ctctatgtcg tagaggggcg tcaggcatgc aagcacctcg atgtcgcacc cgttgcagct
    15841 ggatgcatcg tagtgaagga tccagggtga tcgcttcagg aatgccatac tactcatgca
    15901 cccccgatca tcatatagga gaggatcgcg aggttcgcga tcccgagtgc cgccgcgagc
    15961 cacccgctct tcagcgctgc ctgccagcgg acccgtgcgg tgacgttgtc gatgtagatc
    16021 tcggcaacgt acgtgaccgc gaccgcgatg attgcgatga cggggttcca ggcaaagaag
    16081 aggtagacga ggccgaggag gaagatgttc tcgtaccagt gggctatctc gatctgggcg
    16141 agcgtcgagc ccgagaactc cgtcgtgata cctttgacga tctcctggtg cgcgtggtgc
    16201 gacgtcgata tgtcgaaggg agacttcctg agtttgatcg tgaggatggc ggcaaacccg
    16261 atgaagacgc ccgggaggta ggcgatggcc ggcacggtcg cggccgcgat cgcgtcgacg
    16321 aagaagctcc ccgtgaccat gtagaacccg acgaccacga ggatgatcat cggttcgtag
    16381 gccatcacct ggatcagttc ccgctcggcc ccgatgtggc tgtagggcga gtggaccgcg
    16441 taggccccga ggaccaggaa gacgtgggcg agcgtgaagg caaagaccgt cagcaggagg
    16501 tctcctcccg cgaagaagat cgccccggag agggcgatga agaccaggta ggcgaggacg
    16561 tagaagttct gcgccgtggt gacgacgacc ctctccttct cgaagagttt ccccacatcg
    16621 tagaagggct gcaggatcgg cggcccgacc ctcccctgca tccgcgcggt gatcttacga
    16681 tcgatccccg ctatgaggcc gcccgcgacg ggcgcgagga cgaggaagag gaccgccccg
    16741 ataagccaga gccagttcat aaggccaccc ccgcaaagac ccacgatgcc aggatcagca
    16801 cgatgcagac caccgcgccc ggccaataga gccgcgcctc gccgaagtac ccggtgaggt
    16861 agtagttctg cgtctgggcc tcccgtgtca tgccgaggga gcctgcgaac tgcagttccg
    16921 gcgtcgcggg cctcccgccc atgtagaccg ggaggcgctt tgcgctcttc cggaagaggg
    16981 agaacgatat cgggaggatc aggagcaggg atatcatcag gagcatgatg gcgacgtttg
    17041 cctgcgccag gtgtgcggtc atgccgtata tgacgaagac gtagggttcg atcagcgccg
    17101 tggatatgac cgggaagagg aagacgacca gtatgacgat ggcggtggtt atgtagaggg
    17161 gaacccaggg ctcctccagg aaccctttct cgatccgctc cgtgccccgg gtgaccgcga
    17221 cgagtttgcc catccacttg gcccagaaga agacggtgac ggcgctcccg taggccagga
    17281 tggcgatgaa gacgaggccg aagggcgcct ggacgaacgc ctcgatcgcc gcccatttgg
    17341 agaccagcat accgaacggc gcaaggaaca tgccggcgat cccgatgaac atcatcactg
    17401 ccatcttggg catccggacg atcaggcctt ccatatcctc gatgttgcgg ctcccgatcc
    17461 ggtgctcgat cgccccggtc ccgaggaaga ggagcgactt tgcgacggcg tggaatatga
    17521 tcaggaggat tgccgcccag acgagcatgt atgtcccgac gcccgcacac gcggctatca
    17581 ggccgaggtt tgctatcgtc gagtaggcga ggaccgattt tgcgtcgctt cgcgatatcg
    17641 cgatagcgga ggcgacgacg aaggtcaccg cgccgacgag gccgacggag agcccggcga
    17701 acgtcccggc aaagaccggt gcgaaccgga ccagtatgta gacgccggcc ttgaccatgg
    17761 tgctcgagtg gagcagggcc gagaccggtg tcggggccac catcgccccg aggagccagg
    17821 aggagaaggg catcagggcg gccttcgtga tgccggcaaa cccgatcagg acggccggta
    17881 ccagtatgac ggcggcgtcg ccggaggcaa gcaccagggc gagatcgacg cccccgctcg
    17941 tcgggccggt cgcggcaagg aagatgagcg ccgcgacaaa cgcaaccccg ccgaggaggt
    18001 tcatcaccag ggcgcggaag gcgttcgtcg tcgcctcctc ggtctccgag tagcctatca
    18061 ggacgaacga ggcgatggtg gtgacctccc agaagaagaa gacccacatg aggttgttgg
    18121 agaagaccag gccgaacatc gcggcgagga aggcgaatat gacgaagaag aacatcctct
    18181 gccggtcacg cacctccggg tggtggtggt ggtaggtctc catgtacctg acgccgtata
    18241 cggctatgag gctccctatg atcccgatga tcagggccat gatgatggag aactggtcga
    18301 cgaagaggtt gtttgccgca tggaggctcc ccgcaaaggt cgtctcgagg tataccacca
    18361 gcgccgcctg gacgacggcc agtgcgattg cgaggtagtt tcgaaacttc acgcccatgg
    18421 cgatgatgaa gagcgcaagc gccatctcga tggcaaccat gacgatatcg gtcgcctccg
    18481 aggatgcggc aaagtagacc gcgcccccgg atgcataccg tacgagcagg tataccgatg
    18541 ctccgccgat ggcgagagcc gagagggcga ccaccgcgta tcgcgatgtc gtccgtttca
    18601 cgaacaataa aagacacgcg acgaggatgg gaaagagtat caggaagagc agcgtctgca
    18661 ctattaagtc cctcgtaggg taactatgcc ctacttacat aattaatcat tccaaatatg
    18721 cactccgggg tatcgcccgg ttcgcgcccg gcgccggccc cgcgcgaggg aggcgagccg
    18781 gatctgtcgt ctatcgttcg gataatttgt attgccggct ccgcgcgcgc gaggcgagcc
    18841 gctctctccc cgcgtgcgcc ggggggtaag ggttcgcgcg gtccccaccc ggccgggaga
    18901 gcccgccgcg ggcgtcacca gggcctgcag ggcaggacgc cccgcacgcc tccttcgtgc
    18961 tctccggcgg cgtcgccccg gtagcgcggt atgaagtgga cgtggacgtg catgacgttc
    19021 tgccccgcgg cctctccgac gttgacgcct atgttgtagc cgtcgggggc gaggttatgg
    19081 tcggtcatcg cccggcactc gtcgatgagc cgggcaaggg ccatcttctc ctcgtcggtc
    19141 gtatcgaagt aactcgccac gtgccggaac ggtatgacga gcaggtggcc ggtggtgacc
    19201 gggtggatgt cgtagagggc gtagcagagg tcgtttctcg ccacgatatc ctccggggcc
    19261 gggttgcaga acgggcacgg catggcacct gctcgtctcc cataccagat gagggttttg
    19321 gcccggctcc gctcacccga gcaggcgggc aaggagtctc cccgtgaagc cccgcgagag
    19381 ggcgatggcg tgctcggtgc agatctcgtg gcagcagtag caccggatgc agcgcgagag
    19441 gtcgaccgtc gccctgccct cggtgacggt gatcgcgtgc accgggcaga tacgctcgca
    19501 tttcccgcac ccgatgcacg ccggcgcgat cacgccgggc cggggggcgt aggtcctccc
    19561 gaaccgctgg acgacggcga ggaccacccg ccgccaggtc cccaccctgc cccccgcata
    19621 cgtcgagggt ttccggaagt ccgggacaac gaactccccc gggtcgtcgc cgaccgtccg
    19681 gacggcggaa ggatcgacga ggccgcgggc ggccgcgctc ctggtggtgc cgatctcgag
    19741 cgggtccatg ccgatgagcc gggcaaggac ggtgtccacg gccgcgtagt cgctcccggc
    19801 aaggatcacc ccgatctttc tcgggctgcc ggcctgaggg ccgtcgccct ccatgccgat
    19861 gaccgcgtcc acgatctgga ggcggggttt catgcactcg ttgaggtcga cgagcatact
    19921 cccgaaggcg tactcgtcac ggaaccggaa gtgaaagacc ggtttttcga ggcccggtac
    19981 gagaccgaag aggttctttg cggcacccgt catccgggtc cacatgtggg tctttgcctt
    20041 cgagacgacc acgatcgcgt ctgcctcgag ggccggggcg atgatgggaa actgttttac
    20101 catctcgcct tccgggaaag gtatgacccg gaaaccggtg tcgtagttca ggacggcgtc
    20161 ggtctcctcc gctaccgcgg cgaacccgct ccgcgagtat gcccgccgca ggttcggttc
    20221 gttgtagacg atcccggccc cggggctgtc ggcgatgacg acccggcacc cgcgctccgc
    20281 gaggaggcgg gcgacggcgg cgacgaccgc cgggtgggtg gtgacgcacc gctccggcgc
    20341 ctgtccctgg aggaggtttg gtttgaggag caccttcgcc cccggcgcga cgaaccgctc
    20401 catgccgccg acgagatcga tcgcgcggcg gacggccgca tcgacctcgt ccggcgcgta
    20461 accgtcgcac ctggcgacgg cgacggtcgc atcccgcccg gccggctgca cctcactcct
    20521 ccccgaggcg gccgagggct tctcttgccg cgagcatcgc cgctccctgc ttggtcggcg
    20581 cctcccctct cccgaggggc gtccccccga cgatcacccg ggcgacccag acgggagaac
    20641 tccccggacc cgtccgccgg tagatgtagt cgatcccgcc gaggcccttt cgcgcgacaa
    20701 gctcctggag cctcccccgg tagttccggc gggtgtcgca cccggctatc tcgtcctcga
    20761 atatggcgag gaccacctcc cgggccgcgc cgatgccccg gtcgacgtat atggcgccga
    20821 tgagagcctc gaacgcatcg gcgatgatcg agtcggtcgg cgcctgcccg cgcctcctga
    20881 tgagcccgtc gatgccgagg ccctgccggt gcacgatatc ggcgagtttg gtgttcctcg
    20941 tcacctggag ccgccggttc atctcgccgg gcggaaggtc gtactccccg tagaggtggt
    21001 cggctatgac gaagtcgagg acgtagttcc ccaggaactc gagccgctcg ttgtcgtcgg
    21061 ccgccgctat cgggtactcg gagccgttcg cgtatgaccg gtgggtcagc gcccggtcgt
    21121 agagggggag ggcggcgtcg gcgatatcgg tgatagcaaa cggggggcgg gcgaggagcg
    21181 cgcgcatctc cgcctcccgg tcgcgagagg cgggcccccg gattgcgggg gttctcctcc
    21241 ggaagaagga gccaaaacga agtttttccc ggatggtggc gaggctcatg gtcgtctggt
    21301 gcagggtatc ctgttgttcg tatagagagg ctctcgtgct ctccgataaa cctttgccgg
    21361 gaaacctttt cgtcgtctcc gaatatactt ctctctggta tgcagtccgc ggcggtcggg
    21421 ggcgggggga gcgtgtatct cgatatcgag ttcgggtatg tctacggcac cagccgggcg
    21481 gtcatgatgc cggtcgagat cggcgccgtc atccaccacc cggaagacga cagcgtccgc
    21541 tacgccgggg agcagtttcg ctacgacatc gacgtcgagg tatggaagaa ggtgaccgac
    21601 ccctgcggca ggaccgtcgg cgtcgcgacg accgtggcga atatgggccg cggcaggtac
    21661 ggtggggcct acgaccacta cttccggctc ccgggcgatc gggtccctgc ggcggaagag
    21721 actgccggga aagcgtttgc cgacctccgg gtcttcatgg agtctctcct gacagacgat
    21781 atcacggaga tcgtcgtctt tgcggccgac atggagcgga gggccttccg gaccgcggat
    21841 gtcccgcttg atgggcgccg gctggtcgat ctccagaggg agatacggcg ccgcctcggc
    21901 atgaagcagg tcctctccct cgaccgcctt gcccggctta tcgatttttc cgccgagaat
    21961 ggagccgtcg cctcgacgca ttttcggtac ccggtccccc ccgggtaccg ccacctcctc
    22021 gacgtccacc gggggatggg cgacgccgtc cgtatgttcc tgctcgcgcg ggagttccgg
    22081 gagaagctcc ccgaactgga gaagcgggtc agggctcttg aggatacctg cgggggcgag
    22141 gagtgaccgg tgcgcacggc ccggtcagga tgacgtcgac ctctgctcct ttcggtatag
    22201 ctccgctccc cgccgggacg tgcaggtagg cgtttgcgcg gaccatcgcc atctgcgtcc
    22261 ttgagtgcgg gggcagggga acggcggtat acccgtcgtc ggcccggtat acggtgagga
    22321 ggatgaactc gtcgaactcc ggatcggcca ggaccgattc gcaaagccgg gcacgaaggc
    22381 gttctgccgg cggggcgcaa aaaccccatt ccgcgagaag ggggagggcg agctcgcgta
    22441 tggtcgtcag cgcggcgatc gggtagcccg gcataccgat gaccggtttg tcctcgaccc
    22501 gccccacgat cgccggtctc ccgggcttca tcgcgatgcc gtggacgagc acctcgccga
    22561 gttcgcctat gacgccggcg gtgaagtccc gggtgcctgt cgaggagccg gcggagacca
    22621 ggaccagatc gttctcccgg acggcctttg cgatcgcctg ccggagcgcg gcggggtcgt
    22681 ccgcgacgat gccgtagcgg gtcggagagg cgccggcctc gcaaagccac gccgctgcca
    22741 cggcggtgtt gctctcgatc acctcgcccg cgcccgggtg cccgcccgcc gggaccagct
    22801 cgtttcccgt gggtatgagg ccgaccttca cggccggtac atcaatctcc gcgatgccgt
    22861 aggtgaggag cgcaccgata tcgcaggggc gtatgcggtg gccggccggg aggatcctct
    22921 ctccttgctc gatctcggat cccgcggggt ggatatgttc ccccggtgcc gcggcctttc
    22981 gggtgctcca ggtaccgtcc tctccggcga tatcctcgat catgacgacg gcgtcgtacc
    23041 ccggggggat cgcgtttccc gtgttcaccc ggtgagggtt ccggagccgg acgggggtcg
    23101 catcacccgc cccgcgggtc tcggcgctta tgacggcaaa accgtcccgt gcggcggtat
    23161 ccgttgccgg gaccgtcatg ggcgagtgga cgggccgtgc cgtcacccgt ccggccgatg
    23221 cggcgacggg aaccctcgtc gtccggtccg ggcacgggaa tgtggagcgc atcatggccc
    23281 ggagatcgcc gagcggtttc agggcgaggc gctctctttt ccggtgaaaa aaattagatc
    23341 cgggtgtaga ggtttgcata caccgccttg cccttcgcga tcgggtggcg ctcgccaagg
    23401 tccccgacgt ggtggatgaa gcaggccttc tcgccggcga cctcctgttc cacctcgccg
    23461 accctctgga atcccgggag cgggttctcg atcgttccga agacgtatat gtcgcctttc
    23521 accatctggc cgccgacgcg gcctttggcg ttgcccttga tgatgacggt gccgccttca
    23581 ccgtgggtgg agacgtggat gtcagcgttg ccgccgatga tgatcgtccc gccgttgatg
    23641 aacgtggccg tgtcgttgcc ggcatccccg gcgacccgga tcagcccgcc ctgcatgccg
    23701 cgccactcgc cgcggggcga cgagcccagg tggtgcccgg cgttcccgtc gacgatcagt
    23761 tcgccgcctt ccatccctat gccgcagaac gagtcgacgt tccctttgac gtgaatcttc
    23821 ccgcccttca tccagccgcc gatgtacatg tcggcgttgc cttcgacgac gatctcgcct
    23881 gcggtcatct gctggccgat cctcttcaca cgggtgcagt ccccgcgaaa gacgatcttc
    23941 gtatcggccg cggttgctcc gccgttgccc gagaccgtga agtacttccc gagcgggctc
    24001 ttctgttttc cctcccagac gtcgagggtg gctatctcgg ctgccgtctt gccggcaacg
    24061 ttgtcaggcg tgatgttctg cccctcaaga tagagttcgg ggggatttac cagggtgata
    24121 gttactgtct tcatgcttcc cgtcacctca ctgtgtcgta tcgacctcga tcacccgcgg
    24181 gttgtggagg tgctcatcga agagctggta gttcttgact cccacgctgt agtgcctgag
    24241 gaagtgctcg tagatgtctc tctgcacctg tgcgttctcc ggaaccttca cgtcgaccca
    24301 gatggtccgc tttgccggct ccgtgacgac ttcgccgcct ctggcggtca cgacgccgtc
    24361 tttgacgagg taggcgcacc gcgagaatgc ctcctcgatc ctatcggggt cggagggcat
    24421 attctcgggg ttgagggcgt agacggctat gtcgccatcc atgccgggcg cgagcccgcc
    24481 gtaggtgttg ctgatgccga gcgcctttgc gggtcccgcc cgggtcatct gcgcgatctc
    24541 gtagagcgtg agctcgcggt cgatgctgtc gatggtggtg gactctatcg tccggtcgag
    24601 ccacttgaac gtcttcatgg tcgcgtcacg tgcggccctg ctcatcagcc acttgatgac
    24661 cctcgggtag cgggtgaacg gcccggcgtt cgggtggtcg gtggtgatga acgtccgcat
    24721 caggtcgttg gagtagagcg cgagttccag accgacggcc cactggatgg tggagacctt
    24781 gacgttcggg ctgtagatgt aggggacgac acccgccgcg gtctcgagct cgacgtcggt
    24841 gtttgcccat ttcaggttgt tgaggccccg gaggtggtgc tcgaacggcc cgtcggcggt
    24901 catcgtcgtg gtctcgtcga gtgtcacctg gcccatgtcg atggtgatgt tgtcgtgcgc
    24961 gttcacgtaa tccatgatcg ttttggcctg agagtcgatg ttcttccagg aatcgccgcc
    25021 ataagagcag aactggacgt gcgtgacgtg catgacctga tcgcggccga agttgttctt
    25081 cgccgtgtag ccctctgcaa gcctgaggga gtcgagggtg tcggtgtagt tgcccgggtt
    25141 tccgaggttg ttcgtatgca ggtgcatcga gtgggggagg cccaggaact cgttcgtctc
    25201 gatgagaccc gttatgatct ttgccggggt gatatcgaag taggggaccg tatcgtgtat
    25261 attctcgcag ttcaggcccc atccccaggc ttccgtgccg ccggggttga cgaccttgat
    25321 ggcaaacccg cgggttgccc ggagaagcca ggcggtgtag gcggcattgt tctcgatctc
    25381 gttgttcttg aggtaatcga gcgtgaacca gttgttcccg aagaccggca tcgccgcgtt
    25441 gtcgatgatc ggcgtgtcgc ggatctcctc gtggacgtgg ggggagtgga gcgggggcat
    25501 cgccgcttcg ttgacgaaga cgtagcccat tcgggcgtag tcgtagccgg tcttgaacgt
    25561 ggaggggacg gagaacccca tctccatgcg gttgccccgc ttgagggggc tcttgaagag
//
LOCUS       DAOSII010000014        24059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_2335_length_24059_cov_47.579945, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000014 DAOSII010000000
VERSION     DAOSII010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 24059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24059
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2335_length_24059_cov_47.579945"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..611)
                     /locus_tag="PLV90_02125"
     CDS             complement(3..611)
                     /locus_tag="PLV90_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1673 family protein"
                     /protein_id="HOF95941.1"
                     /translation="MSLVETIRAYLGWCPMAGPVRMDLPVRPGTVAAPGRREGALRVE
                     PGWWNRYHNQLLVMALIASAATAMLFLLIGDTSGYPVVWTGLGFGVGWTIGFLLSYRK
                     QYARITAGEFRRENMSRRQRLVRDLSVPVAAVILIAALAYIVQNGMPGWIVGFMLGLS
                     LIGWTTYGITILWERRHRTTMIAEKRSMYTVDTAIQEETLWR"
     gene            complement(739..1506)
                     /locus_tag="PLV90_02130"
     CDS             complement(739..1506)
                     /locus_tag="PLV90_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95942.1"
                     /translation="MMPILETIRAYLGWCPVKGQVHRQVQPESGMRGDIPDDGRRRVG
                     GVSMKIDFIVKEELWRSIGAVYWVSFLTIGYLILSGYLPISISYAFSLALIPTVAHFL
                     IMVWYRRKENLVDPNPASILRHALGFERQSEKRTDQRRAIIEKVTDAALLVTIFLIYI
                     YASLAYSISQVYWLPVLIVISLFLARIVFTDGGVQRVTVARSVVFYLIAAGFLLLRHL
                     ALGHPVLLYLRGIVLVGIVAFPILYVWERRRTPEGTD"
     gene            complement(1503..1949)
                     /locus_tag="PLV90_02135"
     CDS             complement(1503..1949)
                     /locus_tag="PLV90_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95943.1"
                     /translation="MPLPLNDRQFAFWRLRRSGLQNIHIAERFGISRQAVSKALITMD
                     RKVEETLIAMAQANQIDVERLNAERGVLFGRSVPFDAGAIVFVSADHGVQVWYEHEGD
                     CGACPRYAQCIELLWGYADEMGIALEKTDDPTRLADELFAKLREMV"
     gene            2187..2957
                     /locus_tag="PLV90_02140"
     CDS             2187..2957
                     /locus_tag="PLV90_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HOF95944.1"
                     /translation="MDTALRDAYIRLHERYFPGTELPIAFEVGGPAEGVTRASTPKNW
                     KCFVCDLSKVRRGASLAFDEDSIGCRGGKFYLGYEGERFPDFRYFLSYGKPGVVEGER
                     YKQTPELVDELDRGAVRIPTKGKRIVFKRWDNLAAADNPDAVVFFARPEVLSGLFTLA
                     NFDRADPNGVVSPFGAGCSSIFYYPWLEQQKEDPRAVFGMFDPSARPCVPADVLTMAF
                     PMKKFERVIGFMEESFLTTDTWEKVMKKIARSNALHSP"
     gene            complement(3138..4628)
                     /locus_tag="PLV90_02145"
     CDS             complement(3138..4628)
                     /locus_tag="PLV90_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOF95945.1"
                     /translation="MVLTLEILFLGISLLFLISIIANKFGERLGVPALLIFLIVGMLA
                     GSEGPGGIPFDDPTIAQTIGVIALAYILFSGGLDTRWDEIQPVFWPAIALSTIGVVLT
                     AAVLGAFVVLILGLPPLTGFLLGAVVSSTDAAAVFSVLRMARARLKGNLRPFLEFESG
                     SNDPMAIFLTTGIIALILTPGEPVFALIPMFVQQMIVGGLFGYAMGRFVVWLINRLKL
                     ESEGLYPVLSLAMVLLTYGLTATVGGNGFIAVYIAGLVIGNSIVVYKKSLIRFHDGIA
                     WLMQIIMFLALGLLVFPSELVPVIVPGLLTATFLILVARPVAVMLTLLPWKMPLNEKA
                     LVSWVGLRGAVPVILATYPLVAGVPGADMIFNIVFFIVLVSALVHGTSIPSVARWLGL
                     AAPLQEKRKLSREFDVDPETPSELVEIAISPDAPAVGKQVVDLGLPRGALIILMQKGD
                     ERFVPGGSTVIEAGDTLLLLTTDDLVETVRARLLSGEKPGDTSRAS"
     gene            4864..5952
                     /locus_tag="PLV90_02150"
     CDS             4864..5952
                     /locus_tag="PLV90_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOF95946.1"
                     /translation="MVDTPAFSRSLGTVLLLTAAIFLIIGIQLISYLVTLIVVALILA
                     MLAYPATKVLKSRGLPEGVAVGVVTVVASLLVLLLCYVVFFSFESLIQDLPLFQQELS
                     ARLAEIITTLHGFGIDTGSFSASSINLQQFAGFFSSYAMSLADLLLYLFFIAVTTIFM
                     IFEAPKMPGRIQRVNGDRSGGAETFSRMSRFLIDFVIVRTEANLVHGFLFGSFLWVMG
                     VHAAVLWGVLTFLLSYIPYIGLLVAAIPAIFFAWLQFGLWGAIAVIAAVAVLNALVEN
                     PIFAHFASRRFDIPALVVILSVIFWGWALGIAGMVFAVPLTLIVLLVAQLSADWAWVN
                     TLLGVDHLFTGGQAPPAEGRGGDVPPAE"
     gene            complement(6059..6208)
                     /locus_tag="PLV90_02155"
     CDS             complement(6059..6208)
                     /locus_tag="PLV90_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HOF95947.1"
                     /translation="MAVDPVCKMDVDPATARFKSEYKGKTYYFCAPGCKKLFDKNPES
                     YLKGS"
     gene            complement(6198..8663)
                     /locus_tag="PLV90_02160"
     CDS             complement(6198..8663)
                     /locus_tag="PLV90_02160"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOF95948.1"
                     /translation="MPEEKRKAELKISGMHCASCALNLERALKGRDDVYGARVNFATG
                     TATVEYDPAKATLADLEQTVAGAGYEVMHNEATVRIGGMVCASCAQVIEASLTDLEGV
                     YEARINLATETAHVIYNPELVTTSEIRAAVEDAGYQYLGLAEEVSEDAEALAREEDLR
                     DKFRRFTVGFVVSIPLFFYMIFGMPGAAALPVSVNVVMLIITAPVFYYVSAPIFRAAA
                     AALRNRALTMDVMYAMGIGVAYGASILGTFGLVLTPAFNFYDTAVMLAAFLTLGRYLE
                     ARAKGRTSDAIKNLIGLRPRTATVIRDDREIEVAIEEVVVDDILLVRPGEKVPVDGVV
                     VGGESAVDESMITGEPIPVGKREGDGVVGGTLNVNGVLRIRAEKIGKDMVLSQIIRLV
                     RDAQGSKPPVERIADVAVSYFIPTVLLIATAAFLAWYLALGATLLFSLTVLISVLVVA
                     CPCALGLATPTAVTVGIGRGAEFGVLIRNGEALEISEKLTAVVFDKTGTLTRGKPDVT
                     DIVAFGMPEDRLLAIAAAVEKNSEHPLGAAVVRRAEAAGVTAPASERFRAFGGRGVSG
                     AVEGEEVLVGNRSFLDEHRIPIPPEAEESMAAFQDTGKTAVFVAVSGSFAGIVAVADT
                     LKPTAKTAVADLKRKGLSVTMITGDNPRTANAIAREVGIENVHAGVLPGDKAREVRSL
                     QERGEVVAFVGDGINDAPALAQADVGIAIGSGTDVALESADIVLIRDDLTDAVAAIEL
                     SRKVMGRIKQNLFWAFAYNSALIPLAAGVLYPFFGITFRPELAALAMALSSVTVVSLS
                     LLLKTYIPPAKRGILSEVDAHGS"
     gene            8879..9775
                     /locus_tag="PLV90_02165"
     CDS             8879..9775
                     /locus_tag="PLV90_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOF95949.1"
                     /translation="MASGQDIDGLTEALQNGDPDTRRRAALALSALGEWRAVGPLIRA
                     LADPSQAVREAAANALVMVGTPAVEPLIDLLERPDFTMVYGASREETGEGLTQVDLLG
                     GPEGIPPEKRTLRHGGGITQHDVFGGPEDLKKAGGKRALEHEEEGLTQHDAFGGPEDL
                     KKAGGKQTLEHEEGGIAQHALYAGPEAIPPEKRTLPHRELAQHDLFAGPEGVREHEAL
                     FPGARKAGIVTTGVPPGMKPGLRRAYAAIILGEIADPRAEGILTRSLTDSDPAVRRAA
                     EDGLARFRERRGMATPVPPAPR"
     gene            9877..10770
                     /locus_tag="PLV90_02170"
     CDS             9877..10770
                     /locus_tag="PLV90_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOF95950.1"
                     /translation="MPIEERRPTIEVLRAEKDVAGLIDALGSDDGLTRQRAALALGDL
                     AGAEAVAPLIRALGDPLTAVREAAADSLALLGPPAVGPLVELIESPEASGKYEERTVT
                     VIGAGGRTWELETRQDLRRVYAAAILGEIGDPAAVEPLVRALRDAGDDLRYQAAGAVA
                     KFGSGAVEPLTAVLADPDPDTRIVAVGVLGDTGEAAAVEPLVGALRDGNADVRGAAGG
                     ALIRLGGAAVEPLIAATKDADRNVRLYAAGALKYIGDPRAIEPLRDLARSGDAEEKSV
                     AEDAIERIRTVRRGMPAPTSR"
     gene            complement(10948..11916)
                     /locus_tag="PLV90_02175"
     CDS             complement(10948..11916)
                     /locus_tag="PLV90_02175"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOF95951.1"
                     /translation="MTIITRKGSTPRLPGTRMLMTRDGRRFGTVGGGFCEADAVRAAE
                     GCLASKKSCILRYSSVPGSILPSDIICGGSLEILVEYLPADPYHTRLIGDFVRLRSLR
                     QTPVMIVPLPDGSESGRVRFFIDADGLARGIYPAIHHTQVLKAAREMKRPDVFQAGGM
                     RFLIEPSPLKSRLVICGAGYIAAEIAPLAYHLGFEVSVLDDREDLLSADVYRGTERIP
                     VTSYESCLQALPVDSATDVLIVTRSHAFDLQVLRQALRSPAGYIGMIGSRHKWKTLSS
                     LLLEEGFGDDDLKRVHCPVGLEIGADSPEEIAVSIAAELIEHRALQ"
     gene            12128..12523
                     /locus_tag="PLV90_02180"
     CDS             12128..12523
                     /locus_tag="PLV90_02180"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOF95952.1"
                     /translation="MALREFLGIGCDVEETGRFHLDRTKDAKFLCRFFTAAELDYCFS
                     FEDPAPHLAARYCAKEAVEKALGAAGEEPVEYEDIEIENHSSGAPFITILAKRENLNK
                     KYTIQVSMSHCRTTAMAIATVSILPGEDP"
     gene            12558..13751
                     /locus_tag="PLV90_02185"
     CDS             12558..13751
                     /locus_tag="PLV90_02185"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF95953.1"
                     /translation="MEQSTLNLNDSRRARTLFILYLTGFTTMLGIGIITPVLPIYAQM
                     IGATGFWIGAIFSVFALSRTIFMPFVGRLSDAGGRRKFILLGLAGYAFFSALYVPADN
                     VLILTIVRFFHGMAAAMVYPIANAYVADMARVGEEGKTMGGFQSAAFLGMSFGPLLSG
                     IMVDLLYVQSAFIALAGLSLTALIICIAYLPEYHVPMREIPPLRSVFFSRGMRVPIIF
                     FFTYTAGYTAFMVFFPVLAHADQMTVSGIGALIFISSISTAVFQRLSGRYADLFDRHL
                     VLSTGAGVMAVSLLLFTMSEGFLSFLLTSVLLGAGIGISLTTVSAMAVIEGRNLGQGS
                     VEGVINTAQGIGIVISPLVLGLVMDFTGISMVFLTAAGVSGLMALAMMRFVYRETPAQ
                     ARNAE"
     gene            13887..15185
                     /locus_tag="PLV90_02190"
     CDS             13887..15185
                     /locus_tag="PLV90_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95954.1"
                     /translation="MNIPETAELIVPIAIRNVPIVTGMVEQSATIFGLDKEGAQNLGL
                     AAEEVFNVLASAARPDEKIRIVVRQGGYYVEVACYFSRHAIPITALNITKIPDPDDES
                     DIENLGIILAAKVVDRLQLVRSRQGEMALRFTIERRYPEQVIPSGIPPAGHYSVGEGG
                     FEEIKQFASRVSGMYGANSPGFFRFPGKIVDMVKSREFGVLLALDGKGNVGGGMLWKW
                     GGKMIDSFGPYIFTPQPGLVGEIVQGVLEKVGRKGAVCIVNRMPGDDLLRNFFEELVE
                     SCPANRIRTTACHPPLYRQLEEDTGSVAYVHPEVEIFVKECYERLVLPRSVVATTHAG
                     EFIEPHAAFGVQVDKKQNLAVISTIVAGIDAEEVLAGYVASLYDEGITRLEFEIDLGD
                     ETTPVLVPALHAVGFIPCYVLPWGGRGDILVFECQGPRGF"
     gene            15187..16302
                     /locus_tag="PLV90_02195"
     CDS             15187..16302
                     /locus_tag="PLV90_02195"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOF95955.1"
                     /translation="MAGSLPPLNRLVPPYIQKIEAYIPSKPDDVLKKMYNCPFLYRMN
                     NNENVIGPPEKAIEAIMDFDPARAAIYPSGDGYHLRHRLSEVFGLPSCSFIIGNGANE
                     LIAFVIKAFCWKGDNIITSNKTFAVYEWTAEFSGFESRLVPLKDNTFDDEGILKRIDD
                     RTRVIFICNPNNPTGTYWNEARLRRFLERVDGEQIVVVDEAYAEFVEQPDFPESMRLI
                     REFPNLVVFRTFSKIYGLAALRIGYLAGDRSVVDVIRRTCVVYSVNTLAQEAALAALG
                     DDGTHIARTRSLIRASKEYLLPELDRMDLPYISGEGNFVMIRLPFSDTLAYRRLMRKG
                     YMIRTMTGFRFPNHIRVTLHDVPVMEGFVDALRNTLA"
     gene            16373..17410
                     /locus_tag="PLV90_02200"
     CDS             16373..17410
                     /locus_tag="PLV90_02200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF95956.1"
                     /translation="MTTEPEYTIDDFRPEDAEGVVALYRAVYGENYPIRSVYDAREII
                     RQEESHETYRMVARSPTGEIVGHVAVYHSVPPAPNQELYEAGQLMVRPDYRQTAVAFE
                     LIHACISELPQKHNIGLWGEAVCNHLFTQQMMARERFVETGLEMDLMPESSYSLPTSQ
                     AAGGRVSVIFAFRVCSPHPRTLYLPSIYAESLTFIYEEVKDEYTIALSDAGLPPGTKT
                     KGTMELFKEAGVARITISAVGSDLSDWIGRHGREAAESGIIVTQVFMPLTVPWTGAAV
                     DILRKHGYFIGGALLRWFGGDGLLMQKHAGTPNFDGAHMYTKRAKRIKELVLEDWKAV
                     TEDRRKTEDLQ"
     gene            17476..19140
                     /locus_tag="PLV90_02205"
     CDS             17476..19140
                     /locus_tag="PLV90_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOF95957.1"
                     /translation="MKENADIPADWPEKTTFGAWFRAVAAGAGDRAVLVYRDPARTVT
                     YRELDLLSDAIAKSIIAMGIEKGTHAALWAPNIQEWPAILLGCAKAGLPLLLVNTSFR
                     SYDLEYCLNLVDVGILFLASGNGQPGEYISALYEACPGLGDSQPGKISSQKLPHLRTA
                     VQIDDGNHRGILPWTAFLGQGEQVARTDLQARESSVRPEDRFIFLFTSGTTGWPKCIT
                     WTHERLIGTTFTLADGFGLAPGDVVCIPPPFFHLLGCGILLSALSSSCTVAPLERFNP
                     QEMLRTIETFRVTHIFGVPSMFVSALNEYNRTRYNTASLRGGVVAGAVSPPAVVRAVM
                     DILGAREFGICYGLSEVLATITRPSDPMECRTGSIGRPLRDTDIRIVHPLDGTDVRPG
                     ETGEILVRSPWMISGYYDKPDTIVAATDDDGFLHTGDLGFVDGDGYYHIAGRSRDLII
                     RGGENISPSEIEEFLLTHPAVMDAQVVGIKSEYYGEEPVAFVRTKPGQAVTALGLKQF
                     CRRSIAIEKVPSTFFFVDQYPLTASGKVQKFRLREMAAELLSEQKR"
     gene            19171..19422
                     /locus_tag="PLV90_02210"
     CDS             19171..19422
                     /locus_tag="PLV90_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOF95958.1"
                     /translation="MDESQIEKTLREILARRVPNAPIDKLTLDAPLAALGIDSLAYSW
                     ILADTEDTFGFVMMGSDVMKLKTLKHTIEYVQTQIEKKG"
     gene            complement(19479..20315)
                     /locus_tag="PLV90_02215"
     CDS             complement(19479..20315)
                     /locus_tag="PLV90_02215"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HOF95959.1"
                     /translation="MSEVALPASLEEVYAIRKREPGAAVLAGGTDLLVRMRADGKKPP
                     VLIGLERIPGLSGIAIQDGAVRIGPMVTHQNLLDSPVIRKHLAVMHEAAQVLGSPPVR
                     HMGTIGGNICTASPAGDLLPALYVLDAEVEISSPAKKRVVPIATFITGPGRTILTGQE
                     ILTGIRIPVPDPGYQGFYRKVGQRRALAISIVSMAALLMVSPDKTVRSARFAWGSVGP
                     VVMRFPDVESSFAGRTFGPALLADVGRQYSERIQPISDIRASASYRKMVAAGLPLKIL
                     EQ"
     gene            complement(20308..20778)
                     /locus_tag="PLV90_02220"
     CDS             complement(20308..20778)
                     /locus_tag="PLV90_02220"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOF95960.1"
                     /translation="MKIRCIVNGKQTDLETAPDRRVVDLLREDLGLCGTKEACGSGEC
                     GACTILVDGESRLSCLMIAAQLEGKEIVTIEGFSKEHLHPIQEAFIVHGAVQCGFCTP
                     GMVLAAADLLKRNPAPDRHEIRRAISGNLCRCTGYQKIVDAIESLARAEEESDE"
     gene            complement(20775..23009)
                     /locus_tag="PLV90_02225"
     CDS             complement(20775..23009)
                     /locus_tag="PLV90_02225"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOF95961.1"
                     /translation="MTGRERYAADEYPAGLVYAGVVRSPHPHARILAIDTTGALACPG
                     VIAVLTGGDIRGTNRTGLPEMDQPVLADTKVRFTGDPVVLVVAEDRELLRQAMAAVKV
                     TYEPLPAVFDPDPALAKGAPVLHENRKEGNLLFSGVIAKGDADFAFSECAAVREGIFS
                     LPRQEHAYLETETGVAKVDGDGVLEMIVSTQSPFRDSREIAAALGIPPEKVRVIAPYL
                     GGAFGGKDGITVQGHLGLAALHSGGRPVKIWNSREESFVSSTKRHPGRVSLKLGCTKD
                     GTLHALSCRLIFDTGAYAGLGGPVAVYGMGHAAGVYRVAHSRVEGMNVYTNNPPSGAF
                     RGFGIPQVIAALEQVVDMLAESIGMDPLEFRLKNAIRVGDETGLGIRMSNSADIVTCL
                     EKARDHPLWTGRDEWIRQAPDYKRRGVGIATGVLGIGYGPVIPDIASAKLELLTDGRF
                     RIYAGVADMGQGNNSANIHIAGDILCQSPDRFELVQPDTFRCLNSSSSAASRTTYSYG
                     NALILAAGVLKERILDKVAQLHPLFRRENLILVPGAVRHTPDGSDIALHTIAEAMQEA
                     DRIATATWTAPVNRQQLAVAPDVKMYGFPHLAYTFTTHLTRVEIDEITGAVAVDAYLA
                     WTDAGRIMNPHVYEQQIQGGVIQGMGYALWEDFRVTEGAVVTKNLSTYILPTSMDVPD
                     ILSVPVETYEESGPFGMKGVGEIGIVAALPCIANAVAGAIGSRVCDAPLTPERILTAL
                     RRAA"
     gene            complement(23125..23913)
                     /locus_tag="PLV90_02230"
     CDS             complement(23125..23913)
                     /locus_tag="PLV90_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOF95962.1"
                     /translation="MSIFFWKKIIEYTRRLIGQGDRVQTNYRKVEALSMDELPGADRR
                     MQGREMKQPLCAIILAAGSSSRMGSPKAVLPLGGKTVLEHIVQTYLLAGITDIRVVTG
                     HDREQVRSVLEKIPAGEVNNPRHHEGMYSSVRCGVASCGPEISGYFIHPVDVPLITPR
                     TIRSLCVSADPAGDRIACPVYEGRRGHPPLIGASFGRAILEEDRPQGLKGFLYEYSDA
                     IVPVIVDDPGILLNMNTPADYQEVLRRMENTASDRRGRGNEKTT"
BASE COUNT         4869 a         7311 c         7237 g         4642 t
ORIGIN      
        1 cctcagcgcc atagcgtctc ctcctgtatc gccgtatcca cggtgtacat cgatcttttc
       61 tccgctatca tggttgtccg gtgccgccgc tcccagagga tcgtaatacc atacgtggtc
      121 cacccgatga ggctcaaccc cagcatgaac ccgacgatcc agccgggcat gccgttttga
      181 acaatgtacg ccagagcggc gatgagaatg acggcggcca ccggtacact cagatcccgg
      241 acgaggcgtt gccgccggct catgttctcc ctgcggaact caccggcggt aatccgtgca
      301 tactgcttcc ggtaactgag caggaagccg atggtccacc ccacgccaaa gcccaggccc
      361 gtccacacga ccggataccc cgacgtatct ccgatcagga ggaaaagcat cgccgtcgcc
      421 gccgatgcga tcagcgccat gacaagtagc tggttgtggt agcggttcca ccacccgggt
      481 tcgacccgga gtgccccctc ccgcctgccc ggtgccgcga ccgttcccgg ccgcaccggg
      541 agatccatcc gtaccggccc cgccatggga caccacccga ggtaggcccg tatcgtctcc
      601 acgagagaca tcacgcggcc tcccggagtt tttgcatgcc tgctttcggc tccgtcaaag
      661 agcactcgtt gcagatcttt ctcccgagta tatcccggag gagtacgcag agccgatcca
      721 tcgccccatg ggtcgccatc aatctgttcc ctccggcgta cgccgtcgct cccatacgta
      781 gaggatggga aaagcaacga taccaacaag cacgattccc cggaggtaga gaagaacggg
      841 atgccccagt gccaggtggc gcaagaggag gaacccggcg gctatgaggt agaagacgac
      901 cgatcgtgcg acggtgacac gttgaacacc gccgtcggtg aagacgatcc gcgcgagaaa
      961 caggctgatg acgataagga cggggagcca gtagacctgg ctgatggagt atgcaaggga
     1021 tgcgtagata tagatcagga agatcgtgac gaggagggcg gcatcggtca ccttctcgat
     1081 gatggccctt ctctgatccg ttctcttctc actctgtctc tcaaacccga gggcatggcg
     1141 gaggatgctt gcgggattgg ggtcgaccag attttccttg cgccgatacc agaccatgat
     1201 gagaaagtgc gccacagtcg gtatcaacgc aagggagaat gcataggaga tcgatatcgg
     1261 aaggtacccg ctcaggatga ggtaaccgat ggtaagaaac gatacccagt aaaccgcccc
     1321 aatcgagcgc cagagctctt ctttaacgat gaaatcgatc ttcatactta caccgccgac
     1381 gcgccgtcgg ccgtcgtccg gaatgtcccc gcgcatgccg gactccggtt gcacctgccg
     1441 gtgcacctgc cccttcacgg ggcaccaccc gaggtaggcc cgtatcgtct ccaggatggg
     1501 catcatacca tctcccggag ctttgcgaag agttcgtcgg ccaaccgggt cgggtcatcg
     1561 gtcttctcga gcgcgatacc catctcatcc gcgtagcccc agaggagctc gatgcattgg
     1621 gcatagcgtg ggcatgcgcc gcagtcgccc tcgtgctcgt accagacctg caccccgtgg
     1681 tccgccgata caaagacgat cgcgcccgca tcgaagggaa ccgaacgccc gaagaggacg
     1741 ccgcgttcgg cgttcaggcg ctccacatcg atctggtttg cctgcgccat cgcgataagc
     1801 gtctcctcga ccttccggtc catggtgatg agcgccttcg agaccgcctg gcgggatata
     1861 ccgaaacgct ccgcgatatg gatgttctgg aggccgctgc gccgaaggcg ccagaacgca
     1921 aactgcctgt cgtttaaggg gagaggcatg ggtcaacata cgcaggttga ctatatacgt
     1981 gtttcgatac tctccacgaa ccggggctat gtacagcgcc cggccgccgg ataacccttc
     2041 gtgtgcatcc ctcttcccga agagaccggc gagcgaccgc cggccgggca cgctccggga
     2101 tctccggagg agcccacgca caggtagacg gcccgcccga cagacagacc agaacattta
     2161 accccgccgt cctgaaacag actcacatgg acacagcact gcgcgatgcc tacatccggc
     2221 tccatgagcg ctacttcccg ggaacggagc tcccgatcgc gttcgaggtc ggaggccccg
     2281 ccgagggggt aacgagagct tccaccccga aaaattggaa atgcttcgtc tgcgacctct
     2341 caaaagtccg gagaggggca agccttgcct tcgatgagga ctcgatcggg tgccgcgggg
     2401 ggaagttcta cctcggctac gagggcgagc gattccccga cttccgttac ttcctctcgt
     2461 acgggaagcc cggcgtcgtc gaaggggagc gctacaaaca gacgcccgag ctcgtcgacg
     2521 aactcgaccg gggagccgtc cggataccca cgaagggaaa gcggatcgtc ttcaagcgct
     2581 gggataacct cgcggccgcg gataatccgg acgccgtcgt cttctttgcc cggcccgaag
     2641 tgctctccgg cctatttacg cttgcaaact tcgaccgggc agacccgaac ggggtcgtct
     2701 ctcccttcgg cgccgggtgc agctcgatct tctactatcc ctggcttgag cagcagaaag
     2761 aggatccgag agcggtgttc ggtatgttcg acccctcggc gcggccctgc gtcccggccg
     2821 acgtcctgac gatggccttc cccatgaaga agttcgagcg ggttatcggc ttcatggagg
     2881 agagcttcct caccacggac acctgggaga aggtgatgaa gaagatcgcc cggagcaacg
     2941 cacttcactc gccgtagggc accggactat ccgaccctgc gccggagaac cgggcgggag
     3001 ggatggggcg gcatccgccc ccatacctcg tgcggcaaag cccgcccggc accgggcaca
     3061 cgtgacggcg gcgaagagga gcggggccaa aaagaccgtt gagcaccggc acggccggga
     3121 gagcctcctg ccgctcatca cgatgcccgg gacgtgtcac ccggtttttc tccgctcaaa
     3181 agtctggcgc ggacggtctc caccagatcg tcggtcgtca ggaggaggag cgtatcgccg
     3241 gcctcgatga cggtgctgcc gcccggaaca aaacgttcgt cccctttctg catgagtatg
     3301 atgagcgccc ctctcggcag gccgaggtcg acgacctgct tgccgaccgc cggggcatcg
     3361 ggagatatgg caatctcgac cagctcgctc ggcgtctcgg ggtccacatc aaactcccgg
     3421 gataacttgc gcttctcctg gaggggcgcg gcgagaccga gccaccgggc gacggagggg
     3481 atcgacgttc catgaacgag cgccgagacg aggacgatga agaagacgat gttgaagatc
     3541 atatccgcgc ccggcactcc cgcgacgaga gggtaggtcg cgaggatgac cgggacggcg
     3601 ccccgcagcc ccacccagga gaccagagcc ttctcgttca acggcatctt ccaggggagc
     3661 agcgtaagca tgacggcgac cggccgggca accaggatca ggaaggtggc ggtgaggagg
     3721 ccgggaacga tcaccggcac gagctcggag gggaagacca gcaggccgag ggcgaggaac
     3781 atgatgatct gcatcaacca tgcgataccg tcatggaacc ggatcaggct ctttttgtag
     3841 acgacgatgc tgttcccaat aaccaggccg gcgatataga ccgcgatgaa cccgttccct
     3901 ccaaccgtgg cggtcagccc gtacgtcagc agcaccatgg caaggctgag caccgggtag
     3961 agcccttcgg actccagttt gagacggttg atgagccaga cgacgaatct gcccatagcg
     4021 tagccgaaca ggcccccgac gatcatctgc tgcacgaaca tcgggatgag ggcaaagacc
     4081 ggctcgccgg gggtgagtat cagggcgatg atgccggtgg tgaggaagat cgccatggga
     4141 tcgttgctcc ccgactcaaa ctcaaggaac ggacgcaggt ttcctttcag gcgcgctcgc
     4201 gccatgcgca ggaccgagaa gaccgccgcc gcatcggtgg acgagacgac cgcaccgaga
     4261 agaaaacccg tgagcggcgg caacccgagg atcaggacga caaatgcgcc gaggactgct
     4321 gcagtcagaa caacgccgat cgtagagagt gctatcgccg gccagaagac cggctggatc
     4381 tcgtcccatc gggtatcgag gcccccggag aagaggatat acgcaagcgc gatgaccccg
     4441 atcgtctgtg caatcgtagg gtcatcgaac ggaatgccgc ccggtccttc ggagcccgcc
     4501 agcatcccga cgatgaggaa gatgaggagc gcaggaactc cgagtctctc tccgaacttg
     4561 tttgcaatga tgctgatcag gaagagcaac gatataccga ggaacaggat ctcaagggta
     4621 agcaccatct gtgggtacaa ttcgtgcccg aactatctta aggatgactc cccgctgata
     4681 gaccgggtac cgcgcaccgt gccgatcttt ctgcctgcag cgcgaaacag cgccgcgtag
     4741 attcaatgta cccgggtaac cggtaattta ccggtcattc cgctatattc ctgatgggcc
     4801 gcaccgatac atttcgcggg attgataagg tcgccgcaga cacaggtacc tggtgaaccc
     4861 tggatggtag acactcccgc attctcccga tcgctcggta ccgtgctcct cttgacggca
     4921 gcgatattcc tgatcatcgg gatccagttg atatcgtacc tcgttaccct catcgttgtt
     4981 gcgctgatcc ttgccatgct cgcatacccc gcgacgaaag tgctcaaatc gcgcggcctt
     5041 ccggagggcg tcgccgtcgg cgtggtcacg gtcgtcgcca gccttctggt ccttctgctc
     5101 tgttacgtcg tcttcttctc gtttgagagc ctgatccagg atctccctct cttccagcag
     5161 gagttgagcg cccgacttgc ggagatcatc accaccctcc acgggttcgg gatcgatacg
     5221 ggctcgttct ccgcatcctc gatcaacctg cagcagtttg ccgggttctt ctcttcgtat
     5281 gccatgagcc ttgccgacct gctcctctac ctcttcttca tagcggtgac gacgatcttc
     5341 atgatctttg aagcgccaaa gatgcccggc cgcatacaga gggtgaacgg agaccggtca
     5401 ggaggcgccg agacgttctc ccggatgagc aggttcctga tcgacttcgt catcgtcagg
     5461 accgaggcga acctcgtcca cggtttcctc ttcggttcgt tcctctgggt catgggggtg
     5521 catgctgccg tcctctgggg agtgctgacg ttcctgctct cttacatccc ctacatcggc
     5581 ctgctcgtcg cagcaatccc cgctatcttc tttgcctggc tgcagttcgg gttatggggc
     5641 gcgattgcgg tgatcgctgc cgtcgccgtc ttgaacgcgc ttgtggagaa cccgatattt
     5701 gcacactttg catcgaggcg ctttgacatc cccgccctcg tggtcatcct ctcggtcatc
     5761 ttctgggggt gggcgcttgg catcgccggg atggtgttcg ccgttccgct cacgctcatc
     5821 gttctcctgg tcgcccagtt gagcgccgac tgggcgtggg tgaacacgct cctgggcgtc
     5881 gatcacctct ttacgggagg gcaggcaccc cctgcggagg gcaggggggg cgacgtgccg
     5941 ccggcagagt gatcatcccc tataccatcc tcgcgtgggc aaaacgccgg ccggtgccgc
     6001 gcccgcgcat cggttcgata ccgggcggat ctaatccccg cccggcacaa aaaaaaggtc
     6061 aggacccctt caggtaagat tccgggttct tatcgaagag cttcttgcac cccggagcgc
     6121 agaagtagta ggtcttcccc ttatactcgc tcttgaacct cgccgtcgcc ggatcgacgt
     6181 ccatcttgca taccggatca actgccatgc gcatcaacct ccgatagtat ccctctcttc
     6241 gccggaggta tatatgtttt gagcagcagc gagagcgaga cgaccgtcac cgacgagaga
     6301 gccatcgcga gcgccgcaag ctccggccgg aaggtgatgc cgaagaaggg gtagagcacg
     6361 cccgcggcga gggggatgag ggccgagttg taggcaaacg cccagaagag gttctgcttg
     6421 atccggccca tcaccttccg ggagagctcg atggccgcga cggcgtcggt gaggtcgtcg
     6481 cggatgagga cgatatcggc gctctcgagg gcgacgtcgg tcccgctccc gatcgctatc
     6541 ccgacgtccg cctgcgcgag cgccggtgca tcgttgatcc cgtcgcccac gaacgccacg
     6601 acctcgccgc gctcctggag gctccggacc tccctcgcct tatccccggg cagcaccccc
     6661 gcgtggacgt tctcgatacc gacttcacgt gcgatggcgt tcgccgtccg cgggttgtcg
     6721 ccggtgatca tcgtcaccga taggcccttt ctcttcaggt ccgcaacggc ggtcttcgcc
     6781 gtcggtttca gcgtatcggc aacggcgacg atgccggcaa aactgcccga gactgcaacg
     6841 aagaccgcgg tttttcccgt atcctggaat gcggccatac tctcctccgc ctcgggcggt
     6901 atgggaatgc ggtgctcatc gaggaaggac cggttcccga ccaggacctc ctcgccttcc
     6961 accgcgccgc tcacccctct gccgccgaat gcccggaatc tctccgacgc cggggcggtg
     7021 acgccggccg cctccgcccg ccgcacgacc gccgccccga gcgggtgctc ggagttcttc
     7081 tcgaccgccg ccgcgattgc gagcaaccgg tcctcgggca taccgaacgc gacgatatcg
     7141 gtgacgtcgg gtttcccccg ggtgagcgtc ccggtcttgt cgaagacgac cgcggtcaat
     7201 ttctccgaga tctcgagcgc ttcgccgttc cggatcagca cgccgaactc ggcgcccctc
     7261 ccgatcccga ccgtcacggc ggtcggcgtg gcaagcccga gggcgcaggg gcaggcgacg
     7321 accaggaccg atatcaggac cgtgagcgag aacaggagcg tggcgccgag ggccaggtac
     7381 caggcgagga aagcagcggt cgcgatcaaa aggacggtcg gtatgaagta agagaccgcg
     7441 acgtcggcga tccgctccac cgggggcttt gagccctggg cgtcacggac gagccttatt
     7501 atctgcgaga ggaccatatc cttccctatc ttctcggccc ggatccggag gacgccgttc
     7561 acgttcaggg tgccgccgac gaccccgtcg ccctcccgtt tgccgaccgg gatgggttcg
     7621 ccggtgatca tcgattcgtc gaccgcgctc tcgccgccca cgaccacacc gtctaccggc
     7681 accttctccc ccggcctgac caggaggata tcgtcgacga cgacctcctc gatggcgacc
     7741 tcgatctccc ggtcatcgcg gatcaccgtc gcggtccggg gccggagccc gatgaggttc
     7801 ttgatggcgt cggacgtccg ccctttggcc cgggcctcga gataacgccc gagcgtcagg
     7861 aacgcggcga gcatcaccgc cgtatcgtag aagttgaacg ccggggtgag gacgagcccg
     7921 aacgtcccga ggatgcttgc gccgtaggcg acaccgatgc ccatagcata catgacgtcc
     7981 atcgtcaggg ccctgttccg gagagccgcg gcggccgccc tgaatatggg ggcgctgacg
     8041 tagtaaaaga ccggtgcggt gatgatgagc atgaccacgt tcacggagac cgggagagcg
     8101 gccgcccccg gcatcccgaa gatcatgtag aagaagaggg ggatgctcac cacgaacccg
     8161 acggtgaacc ggcggaactt gtccctgagg tcttcctccc gcgccagggc ctcggcgtct
     8221 tcggagacct cctccgcaag cccgaggtac tggtagccgg catcctcgac ggcggcccgg
     8281 atctcggatg tggtgacgag ttcggggtta tagatgacgt gtgcggtctc ggtcgcaagg
     8341 ttgatccgtg cctcgtagac cccctcgagg tcggtgagcg atgcctcgat cacctgggcg
     8401 caggatgcgc agaccatccc gcctatcctg acggtggcct cgttatgcat gacctcgtaa
     8461 ccggcaccag cgaccgtctg ttcgaggtct gcaagggtgg ccttcgccgg atcgtactcg
     8521 accgtggccg tcccggtcgc gaagttcacc cgggccccat aaacgtcgtc acggcccttg
     8581 agggcacgtt cgagattgag ggcacaggac gcgcagtgca tcccggagat cttcaactct
     8641 gcctttcgtt tctcctccgg catgatgatc ttctcgtccg gtgaagataa atacctgcac
     8701 ctctcatctc cgggcctgta cagccgcacg tgggcgacga aaatctccgg tatgggcgcg
     8761 ccggcattta tacgcaacat tattatataa gcccctggat attaccgccc atcctgaggt
     8821 gatcgtggtg cagggaagga ataatccaga aggagagaag cgacgggatg ttgagcagat
     8881 ggcgtcagga caggatatcg acggcctgac cgaggcgttg cagaacggcg atccggacac
     8941 ccgccggaga gccgccctcg ccctcagcgc gctcggggaa tggcgggcgg tgggccccct
     9001 catccgcgcg cttgccgacc cgtcacaggc tgtccgggag gcagcggcga acgcgctcgt
     9061 gatggtcggg acgccggcgg tcgagccgct gatcgacctc ctcgagcgtc ctgactttac
     9121 gatggtctac ggagcgtccc gggaagagac cggggagggg ctgacccagg tcgacctcct
     9181 cgggggaccc gaaggcatac cgcccgagaa gaggacgctc cggcacgggg ggggcattac
     9241 gcagcatgac gtgttcggcg ggccggagga tctcaagaag gccggcggga agcgggcgct
     9301 cgagcacgaa gaggaagggc ttacgcagca cgatgcgttc ggcggacccg aggatctcaa
     9361 gaaggccggc gggaaacaga cgctcgagca cgaagagggg gggatcgccc agcacgctct
     9421 ctacgcgggc cccgaggcca taccgcccga gaagcggacc ctcccacacc gggagcttgc
     9481 gcagcacgac ctctttgccg gccccgaggg cgtgcgtgag cacgaggcac tcttcccggg
     9541 ggcgaggaag gccggcatcg tgacgaccgg ggtacccccg ggcatgaaac cggggctgcg
     9601 gagggcttac gccgccatca tcctcggcga gatcgccgat ccgcgggcgg aggggatcct
     9661 cacccggtcg ctcaccgaca gcgaccctgc cgtccggagg gcggccgagg acggactcgc
     9721 gcggttccgg gagcggcggg gtatggcgac gcccgtcccg ccggcacccc gctgataccc
     9781 ctccattttc acggtccccc ggaaccggac aaggttctta taccgggcaa ccatcatacg
     9841 ccggatccgg aggcgaagaa attgccggaa aagagcgtgc ccatagaaga gaggcgcccg
     9901 acgatcgagg ttctgcgggc ggagaaggat gttgccggcc tgatcgatgc gctcgggagc
     9961 gatgacggcc tgacccgcca gcgggccgcc ctggcgctcg gggatctcgc cggtgccgaa
    10021 gcggtcgccc ccctcatccg ggcgctcggc gatccgctga cggcggtgcg ggaggcggcg
    10081 gcggactcgc ttgccctgct cggccccccg gcggtcgggc ctctggtcga gctcatcgag
    10141 agccctgaag cgtccgggaa gtacgaggag cgcacggtga ccgtgatcgg ggccggcggc
    10201 cggacctggg agctcgagac ccggcaggac ctccggcggg tgtatgccgc cgccatactt
    10261 ggcgagatcg gcgatccggc agcggtagaa ccgctcgtcc gggcgctccg cgacgcaggc
    10321 gacgacctcc ggtaccaggc ggcgggagcc gtagcaaagt tcggttctgg agcggtagaa
    10381 ccgctcacgg cggtgctcgc cgaccctgac cccgataccc ggatcgtcgc agtcggcgtc
    10441 ctcggggata ccggggaggc ggccgcggtc gagcccctcg tcggtgcgct ccgtgacggg
    10501 aacgccgacg tcaggggggc ggcgggcggc gccctcatcc gcctgggcgg tgcggcggtc
    10561 gaaccgctca tcgccgcgac gaaggatgcc gaccgaaacg tccggctcta cgcggcgggg
    10621 gcgctcaagt acataggcga cccgcgggcg atcgaacccc tccgggacct cgcccggagc
    10681 ggcgatgcgg aggagaagag tgtcgcggaa gacgcaatag agaggatccg gacggtgcgg
    10741 agggggatgc cggcgccgac gtcgcggtaa ccatccaatt tttctgcttt ttcgccggat
    10801 cgtgcaccat aaaggattgc ctgaagcagt cccgcgcccg gcgtttatcc ggccgcggta
    10861 taagagaacg agtgtcgtcg aagcggcggg gtctatcgtg gtctccggtc aaacgaccca
    10921 taaccgtcaa gaacgccgga atgccgatta ttgaagagca cggtgttcga tcagctccgc
    10981 tgcaatgctc accgcgatct cctcggggga atcggcaccg atctcgagac cgaccgggca
    11041 gtgcacccgt ttcaggtcgt catcaccaaa tccttcctcc agcaaaagcg aagaaagagt
    11101 cttccatttg tgcctgctgc cgatcatccc tatataccct gccggactcc ggagggcctg
    11161 ccggagcacc tggaggtcga aggcatggct ccgggtcaca atcaggacgt cggttgccga
    11221 atccacgggc agagcctgaa ggcaggattc gtacgacgta acgggtattc gttccgttcc
    11281 acgatacacg tccgcactga ggagatcctc acggtcatcc agcacggata cctcaaatcc
    11341 caggtggtat gcaagaggag cgatctctgc tgcgatgtag ccggcaccac agataacaag
    11401 ccggcttttt aaggggctgg gctcgatcag gaaccgcatc ccgccggcct gaaagacatc
    11461 cggccgtttc atctcccggg cggctttcag cacctgcgta tgatgaatcg cggggtatat
    11521 cccccgggcc aacccgtctg catcgatgaa gaaccttacc cgaccgcttt cgctgccatc
    11581 cgggaggggc acgatcatca ccggggtctg ccgcaggctc cggagccgta cgaaatcgcc
    11641 gatgagccgg gtgtgatagg gatcggcagg gaggtactcg acaaggatct caagcgatcc
    11701 gccgcagatg atatcagaag gcagtatcga tccggggacg gaggaatacc gcaggatgca
    11761 acttttttta gaggcgagac acccttctgc ggcccggaca gcatcggctt cacagaaacc
    11821 gccgccgacg gtcccgaacc gtctcccgtc ccgggtcatg agcatccggg ttccggggag
    11881 ccggggcgtg gagcctttcc gggtgatgat cgtcatgacg gcaagatccc cgctcgtttc
    11941 cagcaggttg ctgatggtac gataatcgct gctctctgtc gggttcaggg taaccagcct
    12001 ccggataatt cctggttaca ggttgttgat agaatatcca tacggaaaag cataataact
    12061 atctgtcgga tcagatcttt ataccggaac gtacggatat caccgggcgg cccggcggga
    12121 atgcggcatg gccttgcggg aatttttggg aatcggctgc gacgtcgagg agacgggcag
    12181 gttccacctt gacaggacaa aagatgcaaa atttctatgc aggttcttca ccgcagcgga
    12241 gctggattac tgcttctcgt tcgaggaccc cgcgccacac cttgccgccc gatactgtgc
    12301 aaaagaggcc gtggaaaaag cgctcggcgc agcaggagaa gagccggtcg agtacgaaga
    12361 catagagatc gaaaaccact catccggagc gcctttcatc actatcctcg caaaaagaga
    12421 gaatcttaac aaaaagtata cgatccaggt gagcatgtcg cactgcagga cgactgccat
    12481 ggcaatagca actgtatcga tactccccgg ggaagatccc tgaagcctgc gcatcctcat
    12541 aaatagtgtc gtgggatatg gagcagagta ccctgaacct gaatgattcc cggcgagcac
    12601 ggacgctctt catcctgtac ctgaccgggt tcaccacgat gctgggtatc ggcatcatca
    12661 caccggtcct cccgatctat gcccagatga tcggtgccac ggggttctgg atcggggcga
    12721 tattctcggt atttgccctg tcccgtacca tcttcatgcc gtttgtcggc aggctgtcgg
    12781 atgcgggagg gagaaggaag tttatcctgc tcggccttgc cgggtatgcc ttcttctccg
    12841 cactgtatgt cccggcggat aatgtcctca tcctgaccat tgtccggttt tttcacggaa
    12901 tggcagcagc gatggtctat cccatcgcca atgcctatgt cgcggatatg gcgcgggtag
    12961 gtgaagaggg aaagacgatg ggagggttcc agagtgctgc attcctcggg atgagcttcg
    13021 gtccgcttct ctcaggcata atggtggatt tgttgtatgt ccagagtgct tttatcgcac
    13081 ttgcgggact gtcgctgaca gctctgatca tttgcatagc gtacctcccg gagtatcatg
    13141 tcccgatgag agagatcccg ccgctcaggt cggtcttttt cagcagagga atgcgagttc
    13201 ccatcatctt tttctttacc tacacggcgg gatacacggc gtttatggtc ttctttccgg
    13261 ttctcgctca cgcagaccag atgaccgtca gcgggatcgg agcactcatc ttcatctcct
    13321 cgatctcaac agcggtcttc cagcggcttt ccgggagata tgccgatctg ttcgacaggc
    13381 acctggtcct ctccaccggg gccggggtca tggcggtgtc tctgctgctt ttcacgatga
    13441 gcgagggctt tctttccttc ctcctcacct cggtgctcct gggtgccggc atcggtatat
    13501 cgcttacgac cgtatcggca atggcggtca ttgaaggaag gaatcttggc cagggaagtg
    13561 ttgaaggggt catcaacacg gcgcagggga ttggtatcgt tatcagcccg ctcgtcctgg
    13621 gactggtcat ggactttaca ggaatatcga tggtatttct cacagccgca ggagtatcgg
    13681 ggctgatggc actcgcaatg atgcggtttg tatatcggga aacgcccgct caggcccgga
    13741 atgcagaata gaaagattga tattgccgct gcataattga tacacctatt ggaaccgcgg
    13801 gaccaaaaca ggcatgccgg ttccgtgaat cacgaagatc atcgggtgtt tattctcaca
    13861 ataaagctca ggaatggtta gccatcatga acatacctga aacagcagaa ctcatcgtcc
    13921 cgattgcgat ccggaatgtc cctatcgtca ccggcatggt cgaacaatcg gcaactattt
    13981 ttggcctgga caaagagggc gcccagaacc tcgggcttgc agcggaagag gtgttcaatg
    14041 tcctggcatc tgccgcaagg cctgatgaaa agatccggat cgtcgtcaga cagggcggct
    14101 actatgtgga agttgcatgt tatttctcgc gccatgcaat cccgatcacc gcgctgaaca
    14161 tcaccaaaat acccgatcct gacgatgaat cggacatcga aaacctcggc atcatccttg
    14221 ccgccaaggt ggtcgaccgg ctccagctgg tcaggagccg tcagggagag atggctctcc
    14281 gtttcactat cgagcgacgg tatccggagc aggtcatccc ttccgggatc ccgcctgcgg
    14341 ggcattattc ggtcggggag ggagggttcg aagagatcaa gcagtttgct tcacgtgtct
    14401 cggggatgta tggtgccaat tcccccggat tcttccggtt cccgggaaag atcgtggata
    14461 tggtgaagag ccgggaattc ggtgttctcc tggcccttga cggaaaaggc aacgtcgggg
    14521 gcgggatgct gtggaaatgg ggggggaaga tgatcgactc ctttgggccc tacatcttta
    14581 caccacaacc cgggcttgtc ggtgagatcg tgcagggagt actggaaaaa gtgggcagga
    14641 agggtgcagt ctgcatcgtg aacaggatgc cgggcgacga tctcctccgc aatttctttg
    14701 aggaacttgt cgaaagctgc cctgcaaaca ggatccggac cacggcatgc cacccccccc
    14761 tgtatcgcca gctggaggag gatacggggt ccgttgcata tgtccatccc gaagtagaga
    14821 tttttgtaaa agaatgctat gaacggctcg tgctgccccg gagcgtggtc gccacgaccc
    14881 atgcaggaga gttcatcgag cctcacgcgg cattcggcgt ccaggttgat aaaaaacaga
    14941 accttgcggt catttcaacg atagtcgcag gaatcgatgc ggaggaagtc cttgcagggt
    15001 acgtcgcctc tctgtacgac gaggggataa cgcggctcga gttcgagatc gatctcgggg
    15061 acgagacgac tccggtgctg gttcctgctc tccatgccgt gggattcatt ccctgctatg
    15121 tcctgccctg ggggggtaga ggggatattc ttgtcttcga atgccaggga ccgcgaggct
    15181 tctgatatgg ccggcagcct tcccccgctc aaccggctcg tcccgccata tatccagaag
    15241 atcgaggcat atatcccgag caagccggac gatgtcttaa aaaaaatgta caactgcccg
    15301 ttcctgtacc ggatgaacaa caacgagaac gtgatcggcc ctccagagaa agccatcgag
    15361 gcgatcatgg atttcgatcc ggcgagagcg gcaatctacc caagcggcga cggctaccac
    15421 ctgcgccacc ggctcagtga agttttcggc ctcccgagct gttcgttcat catcggtaac
    15481 ggtgcaaacg aactgattgc atttgtcatt aaggcattct gctggaaggg agacaatatc
    15541 atcacgtcca ataagacgtt tgccgtttac gagtggacag cggagttttc cgggttcgaa
    15601 agccggcttg taccgctcaa agacaacacc ttcgatgacg aaggaatcct gaaacggatt
    15661 gatgacagga ccagggtgat cttcatctgc aaccccaaca accccacggg cacgtactgg
    15721 aatgaggcca ggttgagaag attcctggaa cgcgtcgacg gggagcagat cgttgttgtt
    15781 gacgaggctt atgccgagtt tgtcgaacaa ccggattttc cggagagtat gcgtctcatc
    15841 cgggagttcc ccaacctcgt cgtcttccgg acgttctcaa agatatacgg acttgcagcc
    15901 ctgcgcatcg gttatcttgc cggggacaga agcgtggtcg acgtcatccg ccgcacctgc
    15961 gtggtctatt ccgtgaacac gcttgcacag gaggccgcgc tcgcggcgct gggggacgac
    16021 ggcacccata tcgcacggac acggagcctg atccgggcat cgaaggaata tctcctgccc
    16081 gaactggacc ggatggacct cccgtacatc agcggggaag gcaatttcgt catgatccgc
    16141 ctgccgttct ccgatacgct cgcttaccgg aggctcatgc ggaaaggata catgatccgg
    16201 accatgaccg ggttccgatt ccccaaccat atccgggtca ccctgcacga cgtgccggtg
    16261 atggaagggt tcgtggatgc actcaggaac acgcttgcct gacacaacgt gcggatgcag
    16321 tagacccatt tcggtatagc atgagattta atgatgaaca agggatgaaa aaatgacgac
    16381 cgaaccagaa tacacgatcg atgatttcag gccggaggat gcagagggcg tcgttgcact
    16441 ctaccgcgcc gtgtacggcg aaaattaccc gattcgcagt gtatacgatg caagggagat
    16501 catccggcag gaagagagcc acgagaccta ccgcatggta gcccgttcac cgaccggtga
    16561 gatcgtcggg catgtcgctg tttaccattc ggtgcccccg gccccgaacc aggagctgta
    16621 cgaggccggg caacttatgg tacgtccgga ttaccggcag acggctgttg cgttcgagtt
    16681 gatacatgcc tgtataagtg agctcccgca aaaacacaac atcgggctct ggggagaagc
    16741 ggtctgcaac cacctcttta ctcagcagat gatggcgcgg gaaagattcg tggagaccgg
    16801 gcttgagatg gatctgatgc cggaatcctc ctacagcctg ccgacatccc aggctgccgg
    16861 cggccgggta tcggtaatct tcgccttccg tgtatgttca ccgcatcccc ggacactgta
    16921 cctgccatcg atttacgctg aatctcttac attcatctac gaggaagtga aggatgaata
    16981 cactatcgca ctctccgatg ctggcctgcc tccaggtacg aaaacgaagg gcacgatgga
    17041 gctattcaaa gaagccgggg tagcccggat caccataagt gccgtcggca gcgatctctc
    17101 cgattggatc gggaggcacg ggagagaggc tgccgaaagc ggcattatcg tcacccaggt
    17161 cttcatgccg ctcacggtgc cgtggaccgg tgcggcggtc gatatcctgc gaaagcatgg
    17221 atacttcatc gggggagcgc tcctccggtg gttcggcggc gacgggctcc tcatgcagaa
    17281 acatgccggc accccgaatt tcgacggggc gcatatgtac acgaagcggg caaaaaggat
    17341 caaggagctc gtgctcgaag actggaaagc cgtgaccgaa gatcggagga agacggagga
    17401 tctgcaatga aagagaatgc cgacgttccg gccgaccgtc aggggagaga caaacgtccc
    17461 tatcgggaac ctgcaatgaa agagaatgcc gatattccgg ccgactggcc ggagaagacg
    17521 acgttcggtg catggttccg tgccgtggcg gcaggggccg gagacagggc cgtgctcgtg
    17581 taccgcgatc ctgcacggac ggtcacctac cgtgaactgg atctgctctc ggatgcgata
    17641 gcaaaaagca tcattgccat gggtatcgaa aagggcacgc atgctgccct ctgggcaccg
    17701 aacatccagg aatggccggc catccttctc ggatgtgcaa aagccggcct tcctctcctc
    17761 cttgtgaata ccagtttcag atcctacgat ctggagtact gccttaatct ggtggatgta
    17821 ggtattctgt tccttgcttc cgggaacggg cagccgggcg aatatatctc ggcgctgtac
    17881 gaagcctgcc ccggacttgg ggattctcaa ccggggaaga tctcctcgca aaaactgccg
    17941 cacctcagga ccgcggttca gattgacgat gggaaccatc ggggcatcct gccctggacg
    18001 gcattcctcg ggcagggaga gcaggttgcc cgaacagatc ttcaggcacg ggagagttcc
    18061 gtaaggcccg aagatcgctt catcttcctt tttacctcgg ggacaaccgg ctggccgaag
    18121 tgcataacct ggacacatga acggttaatc ggaacaacgt tcactcttgc ggacggcttt
    18181 ggtctcgcac cgggcgatgt cgtctgcatt ccgcccccgt tctttcattt actcgggtgt
    18241 gggatattgc tgagcgcact ttccagcagc tgcacggtag cgcctctcga acggttcaac
    18301 ccgcaggaga tgctgaggac catcgaaact tttcgtgtca cgcatatttt cggggtgcct
    18361 tccatgttcg tgagcgcctt aaacgagtac aaccgaaccc gctacaacac ggcgtcgctc
    18421 agggggggtg tggtcgcggg ggctgtctcc cccccggccg tcgtcagggc ggtcatggat
    18481 atattgggag cgcgggagtt cgggatctgt tatggactca gcgaggttct agcgaccatc
    18541 acccgtccgt ccgatcctat ggagtgcagg acaggcagca tcgggagacc gctgagagac
    18601 accgatatac ggatcgtcca tcccctggac ggtacggacg tcaggcccgg ggagaccggg
    18661 gagatccttg tcaggagccc ctggatgata tccggctatt acgataagcc cgatacgatc
    18721 gttgctgcaa ccgacgacga cgggttcctc cataccggcg acctcggctt tgtggacggg
    18781 gacggctact accatatcgc cggccggagc agggatctga tcatccgcgg cggggagaat
    18841 atatctccct cggagatcga ggagttcctc ctcacccacc ctgccgtcat ggatgcacag
    18901 gtggtcggga tcaagagcga atattacggt gaggagccgg tggcattcgt ccgcacgaaa
    18961 cccgggcagg cggtaacggc cctcgggctc aaacagttct gccggcgcag tattgccatc
    19021 gaaaaagtcc cttctacttt cttctttgtc gaccagtatc ccctgacggc aagcgggaag
    19081 gtgcagaagt tccggctcag ggagatggca gccgaattac tctccgaaca aaaaagatga
    19141 atatatattc tacgagagag ttttagaaat atggatgaat ctcagattga gaagaccctg
    19201 cgggagatac ttgcccgccg cgtaccgaac gccccgatcg ataaactcac cctggacgca
    19261 ccccttgcag cgctgggaat cgattcgctc gcgtactcgt ggatccttgc cgatacggaa
    19321 gatactttcg gattcgtcat gatgggctcg gatgtcatga aactcaaaac actcaaacat
    19381 acgatcgagt acgttcaaac acagatcgag aagaaaggat aaacccggac ggcagcctcg
    19441 ttgaggcgat gccggagggt gcccgtagcc gccttttttc actgctcaag gatcttcagg
    19501 ggcaggccag ccgccaccat cttccggtac gaggcggaag cccgtatatc gctgattggc
    19561 tggatccgtt cggaatattg cctcccgaca tctgcaagaa gcgccgggcc aaacgtcctg
    19621 ccggcaaagg atgattcaac gtctggaaac cgcatgacaa ccggtcccac gctcccccag
    19681 gcaaaccggg cagaacgaac ggtcttatcc ggggagacca tgagcagggc tgccatgctg
    19741 acgatggata ttgcgagggc tctccgctgg cccaccttcc ggtaaaatcc ctgatacccc
    19801 ggatcgggaa cagggatcct gatcccggta aggatctcct ggccggtcag gattgtcctc
    19861 cccgggccgg tgataaacgt tgcaataggg acaacccgct ttttggcagg agaagatatc
    19921 tccacttcag cgtccagcac atacagggca ggaagaagat cgccggcagg cgaggcggtg
    19981 cagatgtttc ccccgatcgt acccatatgg cgtaccggcg gcgatccgag aacctgtgcc
    20041 gcctcgtgca taaccgcgag gtgtttcctg attaccgggc tgtcgagcaa gttctggtga
    20101 gtgaccatcg gcccgattcg gacggccccg tcctgtatcg ctatccctga aagcccgggt
    20161 atccgctcaa gaccgatcag gaccggcggc ttttttccgt cagcccgcat ccgcacgagg
    20221 agatccgtcc ctccggcaag caccgcagca ccgggttcgc gcttccggat cgcatacacc
    20281 tcttcgagcg atgcgggaag ggccacttca ctcatcggat tcctcctctg cccgggccag
    20341 ggattcgatg gcatcgacaa tcttctggta tccggtgcat cggcagaggt tcccgcttat
    20401 cgctctcctg atctcatgcc ggtccggcgc gggatttctt ttcagaagat ctgctgctgc
    20461 aaggaccatg cccggcgtgc agaacccgca ctggactgcc ccatgcacga tgaacgcttc
    20521 ctggatcggg tgcaggtgct cttttgaaaa accttctatt gtcacgatct cctttccttc
    20581 aagctgggcg gcaatcatca ggcaggagag acggctctcc ccatcgacga gaatggtgca
    20641 ggcgccgcat tcccctgacc cgcaggcttc ctttgtcccg cagaggccca gatcctcccg
    20701 gaggaggtcg acgacccttc ggtcgggagc cgtttcaaga tcggtttgct ttccgttgac
    20761 gatgcatcgg atcttcatgc tgccctccgg agagcggtaa ggatccgctc cggcgtaagc
    20821 ggggcatcgc agaccctgga tccgatagcc ccggcaacag cattcgcgat gcaggggagt
    20881 gccgcgacga taccgatctc cccaaccccc ttcatgccaa aaggcccgct ctcctcataa
    20941 gtctccacgg gaacggacag gatgtcaggg acatccattg aggtgggcag gatgtaggtc
    21001 gagaggttct tcgtcacaac agccccttcc gttacccgga aatcctccca cagtgcatac
    21061 cccatgccct ggatgactcc tccctggatc tgctgctcgt atacatgagg attcatgatc
    21121 cgcccggcat ccgtccaggc gaggtatgca tcgacggcca ccgccccggt gatctcgtcg
    21181 atctctaccc tggtcaggtg cgtggtgaac gtataagcaa ggtgggggaa accatacatt
    21241 ttaacgtcgg gtgcaacggc aagctgttgc cggttgaccg gcgcggtcca ggttgctgtt
    21301 gcaatcctgt ccgcctcctg cattgcttct gcgatagtat ggagtgcgat atcgctccca
    21361 tccggagtat gccggaccgc cccgggcaca aggataaggt tctctctccg gaagagggga
    21421 tgcagctgcg cgaccttatc caggatccgt tctttcagca cgcctgctgc gaggataagg
    21481 gcattcccgt acgagtaagt cgtcctgctt gcggccgagg acgatgaatt caggcaccgg
    21541 aacgtgtccg gttggaccag ttcgaaccgg tccggggact ggcagaggat atccccggcg
    21601 atatggatgt ttgccgaatt gttgccctgg cccatatccg cgacccccgc atagatcctg
    21661 aaccggccgt cggtgagaag ttccagtttt gcgctggcga tatcgggaat gaccggaccg
    21721 tacccgatgc cgagaactcc tgtcgcaatg ccgacccctc tccttttgta atcgggggcc
    21781 tgccgtatcc actcgtcccg gcctgtccag agcgggtgat ccctggcctt ttcaaggcag
    21841 gtgacaatat cggcggagtt ggacatccgg attccaagac cggtctcatc gcccacacgt
    21901 atcgcattct tcagcctgaa ctccagcgga tccatcccga tactctccgc aagcatatcc
    21961 acgacctgtt caagggctgc gatcacctgg ggaatcccga acccccggaa tgctcccgat
    22021 gggggattat tggtgtacac gttcatccct tccacacggc tatgagcgac acggtatacc
    22081 cctgccgcat gccccatgcc gtatacggcg accgggccgc caaggccggc ataggcgccg
    22141 gtatcaaaga taagccggca ggaaagggcg tgcagggtgc cgtcctttgt gcatcccagc
    22201 ttcaaggata cccggccggg atggcgtttt gtcgagctga cgaaactctc ttcgcgcgaa
    22261 ttccatatct tgaccggcct tcccccggag tggagggccg caaggccaag gtgcccctgc
    22321 accgtgatgc cgtccttgcc gccaaacgca ccgccgagat acggggcgat tacgcggacc
    22381 ttttcgggtg gaataccgag agccgcggca atctcccggg aatcccggaa cgggctctgg
    22441 gtcgatacga tcatctcgag caccccgtcc ccgtcaacct ttgccacgcc ggtctcggtc
    22501 tcaagatacg catgctcctg ccgcgggagg gagaagatac cctcccggac agctgcacat
    22561 tcggaaaatg caaagtccgc atctcccttt gcgatcacgc cggagaagag cagatttcct
    22621 tccttacggt tttcatgcag gacgggggct ccctttgcga gagccgggtc cgggtcgaat
    22681 actgcaggga ggggttcata cgtcaccttg accgcagcca ttgcctgcct gaggagctct
    22741 ctgtcctcag caacgaccag caccaccgga tccccggtaa accggacctt tgtatcggca
    22801 aggacgggtt ggtccatctc cggaaggccg gtccggttcg tcccccggat atccccgcct
    22861 gtcaggacag cgatgacacc ggggcaggca agcgcccctg tcgtatcgat agcgaggatt
    22921 cgggcatgcg ggtgcggaga cctgacaacc ccggcataca ccagcccggc agggtattca
    22981 tccgctgcat aacgctccct gccggtcacc tttccataag catcggcccg aaagagatct
    23041 gatccgaccg ggaccggcag gtcacttttc gtatgaaaat gcccttcccc tttttcatcc
    23101 ggtatgcctg tggccccatg ggcatcatgt agttttttca ttgccccttc cccggcgatc
    23161 gctggcagta ttttccattc tcctgagcac ttcctggtaa tccgcgggtg tattcatgtt
    23221 caaaaggatt cccggatcat caacgattac cggaacaatt gcatccgaat actcataaag
    23281 aaatcctttg aggccttgtg ggcggtcttc ttcaaggatc gctcttccaa acgatgcccc
    23341 gatgagcggg ggatggcctc gccgcccctc atacaccggg catgcgatac ggtcaccggc
    23401 agggtctgcg gatacgcaaa gagacctgat ggtccggggc gtgatgagcg ggacgtcaac
    23461 cggatggatg aaatacccgg agatctccgg gccgcacgag gcaaccccgc atcggacgga
    23521 cgagtacatc ccctcgtggt gccgggggtt attgacctcc ccggccggga tcttctcaag
    23581 aacggatcgg acctgttcgc gatcatgccc ggtcactacc cggatatccg taattccggc
    23641 gaggagatag gtttgaacga tatgttccag aaccgtcttt cctccaaggg ggagcacggc
    23701 ttttggagag cccatccggg acgatgagcc ggctgcaaga ataattgcgc acaacggttg
    23761 cttcatttcc cttccctgca tcctcctatc cgctcctggc agttcatcca tcgataacgc
    23821 ttcaactttt ctgtaatttg tctgaacccg gtctccctgt ccgatcagtc ttcgcgtata
    23881 ttcaattatc ttcttccaga aaaagatcga cacctaccct cttctcctca cccttaccga
    23941 ctccgcgtga gcgtggagcc cctccgcgag ttgcagcagg acggtaaagc gccgacaggg
    24001 tgatatcgga ggccgtagcg acggtgccgg tgttgcggcg agagcggttc tcaaaaaaa
//
LOCUS       DAOSII010000015        23086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_2457_length_23086_cov_50.018670, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000015 DAOSII010000000
VERSION     DAOSII010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 23086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23086
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2457_length_23086_cov_50.018670"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="PLV90_02235"
     CDS             complement(<1..166)
                     /locus_tag="PLV90_02235"
                     /inference="COORDINATES: protein motif:HMM:NF039182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5654 family protein"
                     /protein_id="HOF95963.1"
                     /translation="MSFKTEVIEKIAALVTAAFGLVAALAWNGAIQELFKLIFGEQST
                     LVAMFVYAVVV"
     gene            410..2422
                     /locus_tag="PLV90_02240"
     CDS             410..2422
                     /locus_tag="PLV90_02240"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOF95964.1"
                     /translation="MDTLDDWFPYREYRPHQREMLEAAASVARDGGIAMIDAPTGSGK
                     SSVISALLAENRGRKVLVAVRTISQLATFMRELELIRKKRGGLKFAYLIGKSSMCPLG
                     GEGDVYRRCEGVKAFSTALMRERAQKGSLVPANDRHIKQQIRKMDPDHPLICPYFIRG
                     KSFVEPGDSGLKMIPSAALRTRAERVSTELIWPDQLAGFCGDICPYDTMMHAARDADV
                     ILVNFYHLFDDTIREQLYLSLGIEGSDALLLIDEAHNCGDVVQSIESVTIEERDIVQA
                     GHELAGRRRSQQSADAVTQILPQITRFMEALKASHEVEDWFDPAIFQRMILAGTLYRN
                     VEGVVDDLLKISEGIREKNMQAGEFRESAIERLTEFFYRIFRSADDPAYLTVYRKADD
                     GSVALEVRNIDPSTKLQEIAQAHACSVLISGTLSPIESYRRYYFGDLPVTTISLPNAF
                     PPENRRIFCADDITTAYSMRRDKENLARTEDYIATFAALPGNLAVYFPSYDLLNTFAE
                     RCAPRIRKKQIYVEPKDTAAASVMLREFMALPGTGRFGVLFAVSGGKWSEGLDYRGEM
                     LAGALVIGLPLAPFNRVRRMVIDYFRMKFGAEGEFISYTLPAINRALQALGRVLRTPE
                     DRGVLVLGERRFLESRVHAGLPPWMQEEMTTCTIDLFKKEAGRWRF"
     gene            2410..2853
                     /locus_tag="PLV90_02245"
     CDS             2410..2853
                     /locus_tag="PLV90_02245"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOF95965.1"
                     /translation="MAVLTGSCRFGLWHVHVAWQDDLVYRVRFARTGLEGPVPEEILL
                     YCAGRRADLSSLRSVATGGSSTFAAIYRAVRDIPSGETATYGDIAQRVGTAPRAVGSA
                     MAKNPTPIVVPCHRVVAKTGIGGFTPDPAIKEALLAMERREKGRS"
     gene            2883..3794
                     /locus_tag="PLV90_02250"
     CDS             2883..3794
                     /locus_tag="PLV90_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HOF95966.1"
                     /translation="MKIVVLGAGAVGLTIAAKLSRVADVHAVARARHADAVRERGLFL
                     TGIWGEGTYRFSCSEDLPPAWRAADYFIITAKSTDTEAVCRQFADAIRGQEVVSLQNG
                     IGNEEVIGRFTDRVIGAMIITGFEWRGDAAVHVSVEAAPMRLGRFPSGTDEAVVRLAS
                     LFSEAGIRAEATGAIRSDLWSKTLYNCALNPLGAIMNVPYGALADPHAWAVVTGIVRE
                     AYLVVEAEGIALPRATPEAYLEYLRTVQLPATAAHHSSMLQDLARGQKTEIEFMNGAV
                     AALARRHGLDAPYNACIAELIRFRERL"
     gene            3807..4442
                     /locus_tag="PLV90_02255"
     CDS             3807..4442
                     /locus_tag="PLV90_02255"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HOF95967.1"
                     /translation="MRSAIILVGGAARRAGGREKYFFTFQGKTFIDRLIDTLREVVDE
                     IVVVARDPEQCERFIHLDDVRCIPDIRRDLGPIGGLHAGTLAAHGEYIFVAACDMPCI
                     NPGVVRMLFDAALGYDAAIPSWNADMLEPLHAVYWRSALLAYLEEHESLSLRPMIRSL
                     NTRYVSVETIREIDPDLLTFTNINKVEDLQLIDPSTEHGQDDACRSGLEPS"
     gene            4779..5912
                     /locus_tag="PLV90_02260"
     CDS             4779..5912
                     /locus_tag="PLV90_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOF95968.1"
                     /translation="MPLIIFCCLAALLCGTATADKGDYAPLWERGADDVVSTVAISHD
                     GSLVAAGAGSTVSLLDREGDILWETAVGSRISDVAISPEGSRIGVAADKLYLFDGDGN
                     LLWAEKTTYIYRGVALSSEGISIAAACDNGALFVFDQDKNILWDYDMGTDGYGVAISE
                     NGRRIALGCDNHGVYYLNSGEGESWSYGTGNCVKGIALTPDGRFVAAGSLDRCVYLST
                     AEGEHLWKYPTTEAVLATALTEEAREVLAASGRTIYVLDQSGAERQKITLGGRVESIA
                     VTPDGSFLAAGGGDGDRSVHLLTKDAALLERMNPVEPAVNETDLPKENVTVPATLADE
                     SADTAAEVNARAGGEEVSLFSTISGWLENILSLLFKPQADFIA"
     gene            complement(5902..6747)
                     /locus_tag="PLV90_02265"
     CDS             complement(5902..6747)
                     /locus_tag="PLV90_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOF95969.1"
                     /translation="MFDVLGFEFFRNALIAGVLASIACGIIGTYVVVRRMVSVSGGIS
                     HAAFGGIGLGYFLGIDPLLGAAGFTVAAALGVGTLQLRARQQMDTLIGAVWAAGMAIG
                     ILFVYLTPGFAPDLFSYLFGNILLVPRGDILLMGILTAVIVAVVAVLYRELQAVTFDP
                     DYAAVMNLPVERLSLLLLVLIALTVVMLIRVVGIILVIALLTLPAAISRLYTARVWTM
                     MLLAVVLGIIFTVAGIALSYVIDVPSGATIVLVSTLAYAGALGAERLREGRGSESAAA
                     ASVRR"
     gene            complement(6750..7517)
                     /locus_tag="PLV90_02270"
     CDS             complement(6750..7517)
                     /locus_tag="PLV90_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF95970.1"
                     /translation="MTAPVIEVDDVWVRLRGRTILEAVSLAIHSGEFYAIIGPNGGGK
                     TTLLRVILGLLAPCRGEVRIGGSTDPAMRKDLGYVPQFRSFDFDYPITVREMVLSGRL
                     GRIARRPRRYGAEDRARTEEALDTMGIADLADRQIRNLSGGEQQRAIIARALVGDPKA
                     LLLDEPTVYVDAPTETQFYEILDGLRDRMAIILVTHDIGVLPGHVTRVACLNRRLYTH
                     DTDEITPDMIEAAYRCPVDLIAHGVPHRVLPEHSREE"
     gene            complement(7514..8368)
                     /locus_tag="PLV90_02275"
     CDS             complement(7514..8368)
                     /locus_tag="PLV90_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOF95971.1"
                     /translation="MKPQATIALYLLVAVLLLTAGCTGVDHREQDAAIVVAVTIAPEQ
                     EFVERVGGDHVRVVLLVPPGADPHTYEPPPGILADVAGADMYAAVGSGVEFELSWKDK
                     IAALNPGMLVVDCSRGVDLISTDPHIWVSPRNAKVMVENICRGLIEVDPENATDYRRN
                     ADAYREELDALDAEIADALARSGVTKIMVYHASWAYFARDYGLVEVPIESEGKEPSPQ
                     RIEHLVRQAEEEGIRVIFASPEHSTRSAEVIAKEVNGTVVQVSPLAGDYLANMRRVAL
                     AFAESGGP"
     gene            complement(8379..8843)
                     /locus_tag="PLV90_02280"
     CDS             complement(8379..8843)
                     /locus_tag="PLV90_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOF95972.1"
                     /translation="MQEITGHELSSKKAEYLKYIYMQGDVVRTTEIAAHFSVAPSTAT
                     KTLADIAKAGYVEQLPYHGARLTARGIDYARFLIRRHRIVALVLSRYGLRPDEACTEA
                     KKIEQCISKDLTDRICTSLGHPMMSGCGEIEHDCFCCPRTSREEDNLESGQT"
     gene            complement(8887..10209)
                     /locus_tag="PLV90_02285"
     CDS             complement(8887..10209)
                     /locus_tag="PLV90_02285"
                     /EC_number="2.8.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase"
                     /protein_id="HOF95973.1"
                     /translation="MCSKEIGRAGAEYSPAERESIQGREAAERESFTIAIDDLRERKV
                     YIESYGCTYNHADARRLAAILEGLGCTLTGPDEADAVIVNTCTVIEATERKMLRRLAL
                     FAGSDLYVTGCMPLVQMEAIRSVCTPHVILPAEIQERAGSVGSRGTGAIGVVQVAGGC
                     VGRCSYCITRLARGRLVSALSEDILDAVRHLVASGAYEIRLTGQDVAAWGLDRGESLP
                     ELLSAITDIPGRFAVRPGMMHPASVLGILKPLIDVYASNKIFGFLHIPVQSGSDAVLE
                     RMQRGYRAADVVGIVDAFRERYPDMMISSDFIAGFPGETDEDFSQTLALLRRAAFVKV
                     NVTRYSRRPGTPAAALKDLPERIRKDRSRALLAEANRIYDSYNERWMGRETSIVATEK
                     NAPETTVCRNPCYLNVVVREDLPFGFSGRAVITENRRHYVVGELLQDI"
     gene            10244..11002
                     /gene="nadX"
                     /locus_tag="PLV90_02290"
     CDS             10244..11002
                     /gene="nadX"
                     /locus_tag="PLV90_02290"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HOF95974.1"
                     /translation="MIKIGLLGCGNVGRIIATRAESIQVVAVFDIIPGRAEELAALCR
                     ARPYTDFDAFMREDFSIVVEAASVDAVKLYGEAVLRSGRDIAVLSGGALADEEFRERL
                     VETARAAGRKIRIPSGAIIGLDNLKIGQVSPPRRLLLRTTKPPASLGLSVAAPTEVFK
                     GLAHDCIKQYPKNINVAVALGLAAGRDADVELRVDPAAERNIHEIFAEGDFGEIYIQV
                     RNVPSPDNPATSYMAALSILTLLKNLDNPLVVGT"
     gene            11005..11898
                     /gene="nadA"
                     /locus_tag="PLV90_02295"
     CDS             11005..11898
                     /gene="nadA"
                     /locus_tag="PLV90_02295"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOF95975.1"
                     /translation="MEAKIRALKAEKNAVVMAHNYQPLDIQALADVVGDSLELAVKAK
                     ETGADLIVVCGVRFMAETAKILNPDRKVVIPVEDAGCPLADFLTPDLIREAKERYPDA
                     AVVVYVNSTAESKALADITCTSANAVRVVMSLPNETILFGPDANLAAYVQRELPKKTI
                     VPVPPGGHCYVHTGFTLADVEAARRKGGTVVCHPECLPEVQEQADVIASTGGMVRGAA
                     GAGEPWSVFTERDMVSRLRVLYPGRVFYEKPEAICVDMKKISPADLQRALECEEHEII
                     LPPGVMDRARRALERMIAVGA"
     gene            11905..12759
                     /gene="nadC"
                     /locus_tag="PLV90_02300"
     CDS             11905..12759
                     /gene="nadC"
                     /locus_tag="PLV90_02300"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOF95976.1"
                     /translation="MIPIEDLLRFIREDAPWGDVTSETVVPDVTCRAVVRAKSQGIVA
                     GLSEARRLFEHFGVTVRERSADGRAVAPGTVLLDLDGPARAILLVERTALNIIGRMSG
                     IATRTREAVEAVRAVSPDVRVAATRKTAPGLRVLDKKAVILGGGDPHRYTLSDMVLIK
                     DNHLALTPLAEAIRRAKKESLYRVVEVEVETVEDAVTAAGAGADIVLLDNMTPDTVRE
                     AVRALAGRGLRDRLTLEVSGGVGEDTLAGYAATGVDIISMGALTHTVRNFDVSLDILK
                     GMGIVRIP"
     gene            complement(12763..13188)
                     /locus_tag="PLV90_02305"
     CDS             complement(12763..13188)
                     /locus_tag="PLV90_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95977.1"
                     /translation="MKSPTGTSEGTTFPPDLERLGIAPGTRIDIRDLDAMGKRHNFHV
                     YLYFEEDLAKDSTLQEDLQEYCDIPDLERPFIRLDAFLRFATESDPLFVRRLDELPLV
                     VEIVAYGEIGIREGKTTPYVKGVMPFLDELAMEELPDAS"
     gene            13280..13834
                     /locus_tag="PLV90_02310"
     CDS             13280..13834
                     /locus_tag="PLV90_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOF95978.1"
                     /translation="MRWPMAGKGASISGGEAADHGNEERLHRHLAMLESGDLNQRWRA
                     ADALGEMGDRRAVQPLIRALSDEFVDVRWKAARALGIIDEREPVLPLIRSLEDESSWV
                     RESAAWALGTIGDPRAVEPLIGVLADTKPRVRREAARALGRIGIPRAREPLVRLLGDP
                     DRGVRLAARAALDDIGTEREIPSV"
     gene            14009..14443
                     /locus_tag="PLV90_02315"
     CDS             14009..14443
                     /locus_tag="PLV90_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOF95979.1"
                     /translation="MDLFMKCAIEEAEMGLREGGIPIGSVLVRGGRIIGRGHNRRVQE
                     NDPVIHAEIDCLRNAGRIGTYADCTLYSTLMPCYLCAGAVVQFGIRKVVAGESANFAG
                     ARELLESHGVEVIDLDLDVCKTMMHAFIEAYPDLWYEDIGEL"
     gene            14458..15264
                     /gene="budA"
                     /locus_tag="PLV90_02320"
     CDS             14458..15264
                     /gene="budA"
                     /locus_tag="PLV90_02320"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HOF95980.1"
                     /translation="MADTFLRYTAVAVAMFVVGASVALLAAPHPESSADRETVFLVST
                     IDALLQGAYDGSVSFDELAAHGDTGIGCGDRLDGELIGLDGEWYLIRTDGRAYPVAGS
                     ATTPFAAATFFDPDVAVAIDEPVNLTALLGRLETMFPSENLFYAVRLDGTFPRVVTRS
                     VPAQEKPYPRLADVVANQTVFEFENVTGTAVGFWTPALAKGINVPGYHLHFITSDRTA
                     GGHLLDMTVAEGSVQLDTTANFTMVLPSRGDFLKVDLSGDLSGDLERVEK"
     gene            complement(15377..15826)
                     /locus_tag="PLV90_02325"
     CDS             complement(15377..15826)
                     /locus_tag="PLV90_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOF95981.1"
                     /translation="MKILVLIDGSKWSQKAALHAIEVAKQKNGEVVLFSVLDIKEAKA
                     MAFNFCTQSGICDRLQDYEGRIWADMRKSTEGDIAGILSRSREENIRCSSKIVEGRVR
                     EEVVSEANSGGYGLVVMGAFGKSGKARIGALIEDIGGVIRPPLLIVR"
     gene            complement(15832..17154)
                     /locus_tag="PLV90_02330"
     CDS             complement(15832..17154)
                     /locus_tag="PLV90_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HOF95982.1"
                     /translation="MDVLALIAVAVFLFTYALIIDERIHRAVAAMFGAAIVVFIGVVP
                     WEGLLEHVDFGTIFLLLGMMIIVNTARGSGLFEYIAIRTAKLANGSPIRILILFALVT
                     AVVSAFLDNVTTVLLLTPMLLYVARVMKLNPVPFLVTEIFASNIGGAATLIGDPPNIM
                     IASSAGLTFNEFLIHLGPIMAVDMVILLLMMYVIYGRSMKVSPGEREEMVRTLDSLDE
                     RAAITDRVLFRQSVIVIAFVVLLFFIHDRIGEILHVVLPFVDPAMGLEPAEVALIGAA
                     VLLFWSRQSPEEIFEKIEWPALFFFGGLFVIVGALVETGIIASIAAVMIENVGSVGEA
                     MFIVTWFAAIASAIVDNIPLTAAMIPLIHDLGTTMDVYPLWWSLALGACLGGNGTAIG
                     ASANVVVIGIAEREGIGITFIDFLKVGMLVLFVTVGVGFGMLWLTFGM"
     gene            complement(17166..17690)
                     /locus_tag="PLV90_02335"
     CDS             complement(17166..17690)
                     /locus_tag="PLV90_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOF95983.1"
                     /translation="MIRRKFKDIAGRRYEAVLKDYSEFLLTDKEMRVPEVRSILIPLD
                     LFVHDITDEAIDVLSAYNATITLAYITDAGVIELLEQALNHASTEEFRAKKEEYGREL
                     LDRTMAKLGDHGFMVQTRMFVGHKGDDVVRMAKNHDMVALCRRYADGESTETSVSPIV
                     LRICQRVETPALIY"
     gene            complement(17815..18345)
                     /locus_tag="PLV90_02340"
     CDS             complement(17815..18345)
                     /locus_tag="PLV90_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin family Zn-dependent
                     metalloprotease"
                     /protein_id="HOF95984.1"
                     /translation="MGINILWDRQAPEGFELPVARMMTMILGREIGFVEYPFLIDGYD
                     RNRNQHDAQKILDRLQDTLTRRYEVAGPLLLVTSRDLYINGCDFVFGLARPASGVAVV
                     STARLGNGYYGRTPDDADLIDRTAKEGAHEIGHLLGLGHCADPECVMFRPQTLDELDR
                     KRKMLCPACREAAGSR"
     gene            18363..18770
                     /locus_tag="PLV90_02345"
     CDS             18363..18770
                     /locus_tag="PLV90_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0146 family protein"
                     /protein_id="HOF95985.1"
                     /translation="MRHYKRIERGVGGYIAGRYRCAVEVGIGNNPDAARVIAAAGGLL
                     LCTDIRPGIRHDGLTVVTDDIFEPDARLYAGADLLYAVRPGVEMVPPLIALAARINCD
                     LLVYHLGCEIYGDGGDVVDCGPALLHRYHRRRS"
     gene            complement(18767..20134)
                     /locus_tag="PLV90_02350"
     CDS             complement(18767..20134)
                     /locus_tag="PLV90_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C large subunit"
                     /protein_id="HOF95986.1"
                     /translation="MDWAEKYRPQRLRDVVGNAGAVRQMYEWARDWSRQKKPLILYGK
                     PGIGKTSSAYALANDMNWEVVELNASDQRTKSALERVAGSGSTTASLLGASRKLILLD
                     EADNLHGQADRGGAKAIVEIIAASQQPIILIANDYYSLSRDLKAATEPVQFRALQARS
                     IVPRLRHICTAEGVTCDPAALDGIAERAGGDMRAAVNMLYAAAIGKESLAAGDVHTSA
                     KDERSTIFELVGAVFKGQDDADLLRTAMEVEDTPDTIVQWLEGNIDYIPDLASREKGY
                     ACLARADGYIGRTFRRQYYTLWRYASAVMLLGVADAAGGRGVRGRIMPPSRWQKMGAS
                     KKQKAVRAGLTRKLSNMAHIPEAALREDLFTAIGILVEQDPKSYVREFELDADELNLF
                     IHDKARAAKIVKELAKEEKAKEKKAGGKEKAAGKEKAPARKTRGDDPPGEARPDLPQG
                     QATLF"
     gene            20371..21381
                     /locus_tag="PLV90_02355"
     CDS             20371..21381
                     /locus_tag="PLV90_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 2 protein"
                     /protein_id="HOF95987.1"
                     /translation="MVKDCSTDTVAQAVREYEGVTRKRAIGDMVRSLRIENPDIVASF
                     GEDAAVIRHNGADALLLAADGIWSKLMEADPFWAGYCAVLVNVHDIAAMGGRPVAMVD
                     ILSVTNDAIRDNVTRGMVAASAQFGVPIVGGHLHPETPYSVVDVAILGTARMDHLIFS
                     DTAEVGDRVVAAIDLDGRVHPSCCFNWDSVTMKSAEAVRAQIRVMEDLGRDRLVTAGK
                     DISNPGVIGTLGMLLEMSGKGAVVDLEAIPRPDLSALDLSFEQWVRMYPGMGFILTVK
                     EENAEEVCRRFTDVGITASLIGEVNGSRKLTIRYLGRETQVFDLDRDGIMQIFSEGDV
                     CR"
     gene            21372..22133
                     /gene="mtxX"
                     /locus_tag="PLV90_02360"
     CDS             21372..22133
                     /gene="mtxX"
                     /locus_tag="PLV90_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker protein Mmp4/MtxX"
                     /protein_id="HOF95988.1"
                     /translation="MPVTVGLGAASPDEKIRATVRAVGREVDLTLFSCPGTAGSFGDA
                     VRVVEAVHPEAALVEALAAGDIDAAVRGTLPASSTLAALKAAAGVDHLERIALLETAE
                     GHLFLLAPVGVDEGWTVADKVRFVEHGRHIARSFGLAGGVAVLSGGRLGDIGRHPAVD
                     RTMADAELVARLTGAEHVEILIEEATRRYGVVIAPDGISGNLIFRTLAFLGAGAGHGA
                     PVVNIDKIFVDTSRASPDYTNAIMLAKSLAESRKP"
     gene            22321..22527
                     /locus_tag="PLV90_02365"
     CDS             22321..22527
                     /locus_tag="PLV90_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone family protein"
                     /protein_id="HOF95989.1"
                     /translation="MADLPIAAVVRIAKKNGAERVGSDAAAALVLKAEAYIAELTKEA
                     NRLAQHAGRKTIKAEDVELAVKSA"
BASE COUNT         4083 a         7375 c         7483 g         4145 t
ORIGIN      
        1 tcacgacaac ggcatacaca aacatcgcaa cgagcgtgct ctgttcaccg aatataagct
       61 tgaagagctc ctgaatggcg ccgttccacg cgagagcggc gaccaggccg aatgcggccg
      121 taacgagggc tgctatcttc tcgatgacct cggtcttgaa ggacatgtgg tttcctcaat
      181 cgggaagtgt acggtaaacg atataatact ttcgcagttc ggctttttcc ggggtttttc
      241 aggcaatagg gcctctatta agagtccaac gggtaaacgg gcggacatat gcgcgatggg
      301 aagcgttccc ggcgggatcg cggggatcag gtccccgccc gccggcgcac ctttcacatt
      361 caccccgcac acccaaacta cattgacgtc ggccgccaac ctgtatgtga tggataccct
      421 cgacgactgg ttcccgtacc gggagtaccg accccaccag cgggagatgc tcgaagcagc
      481 cgcatctgtc gcgcgcgacg gcggtatcgc catgatcgat gcgccgaccg ggagcggcaa
      541 gtcgagcgtg atctctgcgc tcctcgcgga gaaccggggg cgcaaagtgc tcgtcgccgt
      601 ccggaccatc agccagctcg ccacgttcat gcgcgagctc gagctcatcc ggaagaaacg
      661 cggcggcctg aagttcgcct acctcatcgg caagtcgagc atgtgcccgc tcggcggcga
      721 gggggacgtc taccggcggt gcgagggcgt caaggccttc tcgacggcgc tgatgcggga
      781 gcgggcgcag aagggatcgc tcgtcccggc aaacgaccgc cacatcaagc agcagatccg
      841 gaagatggac ccggaccacc cgctcatatg cccctacttc atccgcggca aatcctttgt
      901 cgagccggga gactcagggc tgaagatgat accctcggcg gccctccgca cccgggcgga
      961 gcgggtgagc accgaactga tctggcccga ccagctcgcc gggttctgcg gcgacatctg
     1021 cccctacgac acgatgatgc acgccgcgcg cgacgccgac gtgatcctcg tgaacttcta
     1081 ccacctcttt gacgacacca tccgcgagca gctctacctc tcgctcggca tcgaggggtc
     1141 cgatgccctg ctgctcatcg acgaggccca caactgcggc gacgtcgtcc agagcatcga
     1201 gagcgtgacg atcgaggagc gcgacatcgt gcaggccggc cacgaacttg ccgggcggcg
     1261 gcggtcgcag cagtcggcgg acgccgtcac ccagatcctg ccgcagatca cccggttcat
     1321 ggaagcgctc aaggcgtcgc acgaggtcga ggactggttc gacccggcca tattccagcg
     1381 gatgatcctt gccggcacgc tctaccggaa cgtggaaggg gtcgtcgacg atctcctcaa
     1441 gatcagcgag gggatccgcg agaagaacat gcaggcgggc gagttccggg agagcgccat
     1501 cgagcggctg accgagttct tctaccggat atttcggtct gcagacgacc cggcctacct
     1561 gacggtctac cggaaggcag acgacggatc ggtggcgctc gaggtgagga acatcgatcc
     1621 gagcaccaaa ctccaggaga tcgcgcaggc gcatgcatgc tctgtcctga tatccgggac
     1681 gctctccccc atcgagagtt accggcgcta ctacttcggc gaccttcccg tcaccacgat
     1741 atcgctcccg aacgccttcc cccccgagaa ccgccgcata ttctgcgccg acgacatcac
     1801 caccgcctac tcgatgcgcc gggacaaaga gaacctcgcg cggaccgagg actacatcgc
     1861 cacgtttgcc gccctcccgg ggaacctggc cgtctacttc ccctcctacg acctcctcaa
     1921 cacgttcgcg gagcggtgcg ccccccggat aaggaagaaa cagatctacg tcgagcccaa
     1981 agacaccgcc gccgcttccg tcatgctccg ggagttcatg gccctcccgg ggacgggccg
     2041 gtttggcgtc ctctttgcgg tctctggcgg caagtggagc gagggcctcg actaccgggg
     2101 cgagatgctc gcgggcgcgc tcgtcatcgg cctcccgctt gcgccgttca accgcgtccg
     2161 ccggatggtg atcgactact tccgcatgaa gttcggggcg gagggcgagt tcatcagtta
     2221 caccctcccg gcgatcaacc gcgctctcca ggcgctcggc cgggtgctcc ggacacccga
     2281 agaccggggg gtgctcgtcc ttggagaacg gcggttcctg gaatcccggg tccacgccgg
     2341 tctgccgccg tggatgcagg aggagatgac gacctgcacc atcgacctct tcaagaagga
     2401 ggccggccga tggcggttct gaccggatcg tgccggttcg gcctctggca cgtccacgtc
     2461 gcgtggcagg acgatctcgt ctaccgggtg cgttttgcac gcaccgggct tgagggcccg
     2521 gtcccggagg agatcctgct ttactgtgcc gggcgccggg cggacctctc ctccctccgg
     2581 agtgttgcga ccgggggaag ttcgacgttc gccgcgatct atcgcgcggt tcgcgacatc
     2641 ccctccggag agaccgccac ctacggcgat atcgcccaaa gggtcgggac cgcgccccgg
     2701 gcggtcgggt cggcgatggc taagaacccg accccgatcg tggtgccctg ccatcgggtc
     2761 gtcgcaaaga ccggtatagg cgggttcacc cccgatccgg ccatcaagga ggcgctgctc
     2821 gccatggagc gccgggagaa agggagaagt taacaggtaa ctgcgtctat ataggtagtg
     2881 acatgaagat cgtggtcctg ggtgcgggag cggtcggcct gaccatcgcc gcaaagctct
     2941 cccgagtcgc agatgtccat gccgttgcga gggcgcggca cgcggacgcg gtgcgggagc
     3001 ggggtctctt tttgaccggc atatggggag agggcaccta ccgcttcagt tgctctgaag
     3061 accttccccc cgcgtggcgg gctgccgact acttcatcat caccgccaaa tccaccgata
     3121 cggaggcggt ctgccgccag tttgcggatg caatccgggg acaggaggtg gtgagcctcc
     3181 agaacggcat cgggaacgag gaggtcatcg ggcggttcac cgaccgggtc atcggggcca
     3241 tgatcatcac cgggtttgag tggcggggcg atgcggcggt tcacgtctcg gtggaggcgg
     3301 cgccgatgag gcttggcagg ttcccgtccg gcacggacga ggcggtcgtc cgccttgcct
     3361 ctctcttttc ggaggccggc atccgggcgg aagcgaccgg tgcgatcagg agcgacctct
     3421 ggtcaaaaac cctctacaac tgtgcgctaa acccgctcgg ggccatcatg aacgttccct
     3481 acggggcgct tgccgatccg cacgcctggg cggtcgtcac cgggatcgtc cgggaggcct
     3541 atttggtggt ggaggccgag ggcatcgccc tcccccgggc aacgccggag gcgtacctgg
     3601 agtatctccg gaccgtccag ctccctgcga cggccgccca ccactcctcg atgctccagg
     3661 acctcgcccg cgggcaaaag accgagatcg agttcatgaa cggcgccgtg gcggcgctcg
     3721 cccggcggca cggtctcgat gcgccctata acgcctgcat cgccgaactg atccgcttcc
     3781 gggagcggct ctagcgcagg gtgagcatgc gatcggcaat tatcctcgtg ggcggagcgg
     3841 cacggcgtgc cggagggcgg gagaagtact tcttcacctt ccagggcaag accttcatcg
     3901 accgcctcat cgataccctg cgggaggtgg tggacgagat cgtcgtggtc gcgcgggacc
     3961 cggagcagtg cgaacggttc atccatctcg acgacgtcag gtgcataccg gatatcagga
     4021 gggatctcgg gcctatcggc ggcctgcacg cggggacgct tgcggcgcac ggcgagtaca
     4081 tattcgttgc cgcctgcgac atgccctgca tcaaccccgg ggtggtgagg atgctctttg
     4141 acgccgctct cgggtacgac gccgccatac cgagctggaa cgccgatatg ctcgaaccgc
     4201 tccatgcggt ctactggagg agcgcgctgc tcgcctacct ggaggagcac gagtccctct
     4261 cgctccgccc gatgatccgg agcctgaaca cccgttacgt gagcgtcgag acgatccgcg
     4321 agatcgaccc cgacctcctg acgttcacga acatcaacaa ggtcgaggac ctgcagttga
     4381 tcgacccgtc gacggagcac gggcaggacg atgcgtgcag gtccgggctg gaaccgtcct
     4441 gatccggccc ggaccgcgcc gtctcccggt tctctgcggc ccgaggcgat gacctccccg
     4501 gcccccggcg ccaaatgtcc cgcgggatac ccccgtgccc cggggctccg ccgcgccgcc
     4561 tgtcgtggga ttgcacgggt gccggtctgc cgtccgggga gggcgggcct cctcccgacc
     4621 gaaaggctta taccggttat ctgtgtagtc agggcctgat gtcatcgggg gaagtgactt
     4681 caaccagatc taccggaggg gggagggttt actccagggc cacgccaccc acgcgctccg
     4741 actcgaaaag ccgccggccg ggtgctgccc ggctttctgt gccgcttatc atattctgct
     4801 gtcttgccgc tctcctgtgc gggaccgcta ccgccgataa gggagactac gcccctctct
     4861 gggagagggg ggccgacgat gtcgtcagta ccgtagcgat ctcccacgac ggttctctgg
     4921 tcgctgccgg tgcgggctcg acggtctccc tcctggaccg ggagggtgat atcctctggg
     4981 agaccgcggt cggctcccgg ataagcgacg tcgccatatc gcccgaaggc tcacgcatcg
     5041 gcgttgccgc ggataaactc tacctctttg acggtgacgg gaacctgctc tgggccgaga
     5101 agacgacgta tatctaccgg ggcgtggccc tctcgtcaga aggcatctcc attgccgctg
     5161 catgcgacaa cggcgctcta ttcgtctttg accaggataa gaatatactc tgggactacg
     5221 atatgggcac cgacggttac ggcgtcgcca tatcggagaa cggccggagg atcgcgctcg
     5281 ggtgcgacaa ccatggggtc tactacctca acagcggcga gggggagtcc tggagttacg
     5341 ggaccggtaa ctgcgtaaag ggcatcgccc tcactcccga cggtcggttt gtggccgccg
     5401 gatctcttga ccggtgcgtc tacctctcca cggcggaggg ggagcacctc tggaagtatc
     5461 ccaccacaga ggccgtcctt gcaacggccc tgaccgaaga ggcgcgagag gtcctcgccg
     5521 catcgggcag gacgatctac gtcctcgatc agtcgggggc cgaacgccag aagatcaccc
     5581 tcggcggtcg cgtggagtcg atcgccgtga cccccgacgg ttcgttcctt gccgccgggg
     5641 gcggcgacgg cgatcggtcc gtccacctcc tcacgaagga tgcggccctg ctcgagcgca
     5701 tgaatccggt ggagcctgcg gtgaacgaga ccgatctccc gaaggagaac gtgaccgtcc
     5761 ccgcgaccct tgcagacgag agcgcggata cggcggcaga ggtgaatgcc cgggccgggg
     5821 gtgaggaggt ctccctcttc tcgacgatct cgggctggct ggagaacata ctctccctgc
     5881 tcttcaagcc ccaggcggac ttcatcgcct gaccgacgcg gcggccgcgg actcgctccc
     5941 cctcccctcc ctgaggcgct cggccccgag agcgcctgcg tacgcaagcg tgctcacgag
     6001 aacgatcgtc gcgcccgacg gaacatcgat gacgtaggag agggcgatcc ccgcgacggt
     6061 gaagatgatg ccgaggacga cggcaaggag catcatggtc cacacgcgtg cggtgtagag
     6121 gcggctgatc gccgccggga gcgtgaggag cgcgatcacg aggatgatcc ccaccacccg
     6181 tatcagcatc accaccgtga gagcgatgag caccaggagg aggagcgaga gccgttcgac
     6241 gggaagattc atgaccgccg cgtaatccgg gtcgaacgtg accgcctgga gctcccggta
     6301 gaggacggcg acgacggcga cgatgacggc ggtgaggatc cccatcagca ggatatcccc
     6361 ccgcggcacg aggaggatgt tcccaaagag gtaggagaag aggtcgggcg caaaccccgg
     6421 tgtcaggtag acaaagagaa tccctatagc catcccggcc gcccagaccg cgccgatgag
     6481 ggtatccatc tgctgccggg cccggagctg gagcgtgccc acaccgagcg ccgccgccac
     6541 ggtgaacccg gcggcgccga ggagcgggtc gatgccgagg aagtagccaa gacctatccc
     6601 cccgaaggcc gcatgcgata tgccgccgct gaccgagacc atccgccgca ccacgacgta
     6661 ggtgccgatg atgccgcagg cgatactcgc gagtacgccg gcgatgaggg cgtttctgaa
     6721 gaactcaaac ccgagcacat caaacatgct cactcctccc gcgagtgttc gggaagtacc
     6781 cggtgcggca caccgtgggc gatcaggtcc accgggcagc ggtaggccgc ctcgatcatg
     6841 tcgggcgtga tctcgtccgt gtcgtgcgtg tagaggcgcc ggttcaagca ggcaacccgg
     6901 gtcacgtgcc cggggagcac cccgatatcg tgagtcacga ggatgatcgc catccggtcg
     6961 cggagcccgt ccaggatctc gtagaactgc gtctccgtcg gggcatccac gtagacggtc
     7021 ggctcgtcga ggaggagcgc cttcgggtcg ccgacgagcg cccgggctat gatcgcccgc
     7081 tgctgctccc cgcccgagag gttccggatc tgccggtcgg caagatcggc gatccccatc
     7141 gtatcgagcg cctcctccgt ccgggcacgg tcttcggccc cgtagcggcg cgggcggcgg
     7201 gcgatgcgcc cgaggcggcc ggagaggacc atctctctga cggtgatggg gtagtcgaag
     7261 tcgaacgacc ggaactgggg gacgtacccg aggtcctttc gcatcgccgg atcggtgctg
     7321 ccgccgatcc ggacctcccc ccggcagggg gcgaggaggc cgaggatcac ccggaggagc
     7381 gtcgtctttc cgccgccgtt cggcccgatg atggcgtaga actcgcctga atggatggcg
     7441 aggctcaccg cctcaaggat ggtccggccg cgcagcctga cccagacgtc gtcgacctcg
     7501 atcacgggag cggtcatggt ccgccgctct ccgcgaatgc cagggcaacg cgccgcatgt
     7561 tcgcaaggta atcccccgcg agcgggctta cctgcaccac ggtgccgttg acctcttttg
     7621 cgatcacctc ggcgctccgc gtggagtgct cgggcgacgc gaagatcacc cgtatcccct
     7681 cctcctctgc ctgcctgacg aggtgctcta tcctctgcgg ggagggctct tttccctcgc
     7741 tctcgatagg aacctcgaca aggccgtagt cccgggcgaa gtaggcccac gacgcgtggt
     7801 agaccatgat cttcgtcacg ccggaccggg cgagcgcatc ggcgatctcc gcatccaggg
     7861 cgtcgagctc ctcccggtac gcgtcggcgt tccggcggta atcggtggca ttctccgggt
     7921 cgacctcgat gagcccccgg catatgttct cgaccatcac cttcgcgttc cgtggcgaga
     7981 cccatatgtg cggatcggtc gatatcaggt cgacgccgcg cgagcagtcg acgacgagca
     8041 taccggggtt cagggcggcg atcttatcct tccaggagag ttcgaactct acccccgatc
     8101 ccaccgcggc atacatatcc gcccccgcga cgtcggcaag gatacccggc ggcggttcgt
     8161 aggtgtgggg gtcggccccc ggcggcacca ggaggacgac gcggacgtgg tcgcctccca
     8221 cccgctccac gaactcctgt tcgggggcta tggtgaccgc gacgacgatc gctgcatcct
     8281 gctcgcggtg gtcgactccg gtgcagcccg ccgtgagcag gagaactgca acgagcagat
     8341 agagcgcgat agtagcctgt ggtttcatcc tctccacctc aggtttggcc cgactctaaa
     8401 ttatcttcct cccggctcgt tctcgggcag cagaagcagt cgtgctcgat ctcgccgcag
     8461 ccgctcatca tcgggtgccc aagcgacgtg catatcctgt ccgtgaggtc ttttgagatg
     8521 cactgctcga tcttctttgc ctcggtgcag gcctcgtcgg gcctgaggcc gtagcggctg
     8581 aggacgagag caacgatccg gtggcggcgg atcaggaacc gggcgtagtc gatgccgcgg
     8641 gccgtgagac gcgccccgtg gtagggcaac tgctcgacgt agcccgcctt cgcgatatcc
     8701 gcgagcgtct ttgtcgccgt cgagggcgcg acggagaaat gagcggcgat ctcggtcgtc
     8761 ctcacgacat cgccctgcat gtagatgtac ttgaggtatt cggccttctt tgaagagagt
     8821 tcgtgcccgg tgatctcctg catgaagagt ggaaggatct ctatccggga aaagttttcg
     8881 gtgtggctaa atatcctgca ggagttcgcc gacgacgtag tggcgccggt tctccgtgat
     8941 caccgccctc ccggagaacc cgaacggcag gtcctctctg acgacgacgt tgaggtagca
     9001 ggggttgcgg cagaccgtcg tttcgggcgc gttcttctcg gtcgcgacga tcgaagtctc
     9061 ccggcccatc cagcgctcgt tgtaggagtc gtagatccgg ttcgcttcgg cgagcagcgc
     9121 ccgggaccgg tcttttcgta tccgctcggg gaggtctttg agggcggcgg cgggcgtgcc
     9181 gggccgccgg gagtagcggg tgacgttcac cttcacaaac gccgcccgcc gcaggagggc
     9241 aagcgtctgc gaaaaatctt catccgtctc ccccggaaac ccggcgatga agtctgaaga
     9301 gatcatcatg tccgggtacc gctcccggaa cgcatcgacg ataccgacga cgtccgccgc
     9361 gcggtaaccg cgctgcatcc gctcgaggac ggcatcggag cccgactgga cggggatgtg
     9421 gaggaaccca aagatcttat tgcttgcgta aacgtcgatg aggggcttga gtatcccgag
     9481 caccgaggcg gggtgcatca tgcccggccg gacggcgaac cttcccggta tatccgttat
     9541 cgccgagagg agttcgggga gcgactcgcc ccggtcgagc ccccaggcgg ccacgtcctg
     9601 cccggtcagc cggatctcat aggcccccga cgccacgagg tgccggacgg catcgagtat
     9661 gtcctccgaa agcgcactta caagccgccc ccgtgcaagg cgggtgatgc agtaactgca
     9721 ccggccgacg cacccgcctg ccacctgcac cacgccgatc gcgccggtgc cgcggctgcc
     9781 gacgctcccg gcacgctcct ggatctcggc cgggaggatg acgtgcggcg tgcagaccga
     9841 ccgtatcgcc tccatctgca cgagcggcat gcacccggtc acgtagaggt cggagccggc
     9901 gaagagggca aggcgccgga gcatctttcg ctcggtcgcc tcgatcaccg tgcaggtgtt
     9961 gacgatcacc gcgtccgcct cgtccggccc ggtcagcgtg cacccgagcc cctccagtat
    10021 cgccgcaagc ctccgggcgt cggcgtggtt gtaggtgcac ccgtaactct cgatgtagac
    10081 ctttctttcc ctcagatcgt cgatggcgat ggtaaacgac tccctctctg cggcctctct
    10141 gccctgtatg ctctctcgtt cggccggaga atactctgcc cccgcccggc cgatctcttt
    10201 tgagcacaat acttaaccgc tactattaca acttctttac tcaatgatta agatagggtt
    10261 gctgggatgc ggcaacgtcg ggcgtatcat cgccacgcgc gccgagagca tccaggtcgt
    10321 cgcggtcttc gatatcatcc ccgggcgggc ggaggagctc gcggcgctct gccgggcccg
    10381 gccatacacg gactttgacg ccttcatgcg ggaggacttc tcgatcgtcg tggaagccgc
    10441 ctcggtcgac gccgtcaaac tctacggcga ggcggtcctc cggtcgggac gggacatcgc
    10501 cgtcctctcc gggggagcgc tcgcggacga agagttccgc gaacgcctcg tcgagaccgc
    10561 acgggcggcg ggaaggaaga tccggatacc gagcggcgcc atcatcgggc ttgacaacct
    10621 caagatcggc caggtctcgc cgccgagaag actcctcctc cggacgacaa aaccccctgc
    10681 gtcgcttggc ctctccgtcg cggccccgac agaggtattt aaggggctgg cgcacgattg
    10741 tataaagcaa tacccgaaga acatcaacgt cgcggtggcg ctgggactcg ctgccggccg
    10801 ggatgccgat gtggagctcc gtgtcgaccc cgcagcggag aggaacatac acgagatatt
    10861 cgccgagggc gacttcgggg agatctatat ccaggtcagg aacgtcccga gccccgataa
    10921 ccccgccacg agttatatgg cggccctctc catcctgaca ctcttgaaga acctcgataa
    10981 ccctctggtg gtgggcacat aggaatggaa gcaaagattc gtgcgctcaa ggccgagaag
    11041 aacgcagtcg tcatggcgca taactaccag cccctggata tccaggcgct cgccgacgtg
    11101 gtgggcgaca gcctcgagct tgcggtgaag gcaaaagaga ccggggcgga cctgatcgtc
    11161 gtctgcggcg tccggttcat ggccgagacg gcaaagatct taaaccccga ccggaaggtc
    11221 gtcataccgg tggaggacgc ggggtgcccc cttgccgact tcctgacccc cgacctcatc
    11281 cgggaggcaa aagagcggta tcccgacgcc gccgtggtgg tctacgtcaa cagcacggcg
    11341 gagagcaagg cgctcgccga tatcacctgc acatccgcaa acgcggtcag ggtcgttatg
    11401 tctctcccga acgagaccat cctcttcggg ccggatgcaa accttgcggc atacgtccag
    11461 cgcgagctcc caaaaaagac gatcgtaccg gtgccgcccg gcggccactg ctacgtccac
    11521 accgggttta cccttgccga cgtcgaagcg gcccggagga agggcggtac ggtcgtctgc
    11581 caccccgagt gcctgccgga ggtccaggag caggcggacg tgatcgcatc cacggggggc
    11641 atggtccggg gtgcggcggg agccggcgag ccctggtcgg tcttcaccga gcgggatatg
    11701 gtctcccgcc tccgggtgct ctacccggga agggtctttt acgagaaacc ggaggcgatc
    11761 tgcgtggata tgaagaagat atcccccgcc gatctgcaaa gggcgctcga gtgcgaggag
    11821 cacgagatca tcctccctcc cggggtcatg gaccgggcgc ggcgggctct cgagcggatg
    11881 attgccgtcg gggcgtgagt gcgtatgatt cctatcgagg acctgctccg gttcatccgg
    11941 gaggatgcgc cgtggggcga cgtcacgtcg gagacggtcg tccccgacgt cacctgccgg
    12001 gcggtcgtcc gggcgaagag ccaggggatc gtcgcggggc tctctgaggc gcgacggctc
    12061 tttgagcact tcggcgtcac ggtccgcgag cgctccgccg acggcagggc agtcgccccg
    12121 ggaacggtcc tcctcgacct cgacggccct gcccgggcga tcctcctcgt ggagcggacg
    12181 gcgctcaaca tcatcgggcg gatgagcggt atcgcgaccc ggacccgtga ggcggtcgag
    12241 gccgtccggg cggtctcgcc ggacgtccgg gtcgccgcga ccagaaagac ggctccgggc
    12301 ctccgggtgc tcgacaagaa agcggtgatc ctcggcgggg gcgacccgca ccgctacacg
    12361 ctctcggata tggtcctgat caaggataac cacctcgcgc tcacgcccct tgcggaggcg
    12421 atacggaggg caaagaagga gagcctctac cgggtggtcg aggtggaggt cgagacggtg
    12481 gaggatgcgg tcacggcagc cggcgccggg gccgatatcg tcctgctcga caacatgacc
    12541 cccgataccg tccgggaggc cgtccgggcg cttgccgggc ggggtctccg ggaccgcctg
    12601 actctcgagg tctccggggg ggtcggggaa gacaccctcg ccgggtatgc cgcaaccggc
    12661 gtggatatca tcagcatggg tgcgctcacc catacggtca ggaacttcga cgtgagcctg
    12721 gatatcctga aaggtatggg catagtccgg atcccgtgac cttcaggatg cgtccgggag
    12781 ttcctccatc gcgagttcgt cgaggaacgg catgaccccc ttgacgtagg gcgtggtctt
    12841 cccctcccgt atccctatct ccccataggc gacgatctcc acgaccagcg ggagttcatc
    12901 caggcgccgg acgaagagag ggtccgactc ggtggcgaac cgcaggaagg cgtccagcct
    12961 gatgaacggg cgctccaggt ccgggatgtc gcagtactcc tgcaggtctt cctggagcgt
    13021 ggaatccttt gccaggtctt cctcgaagta gaggtagacg tggaagttgt ggcgttttcc
    13081 catcgcatcg agatccctga tgtcgatcct cgtgccgggg gcgataccga ggcgttccag
    13141 gtccggcggg aacgtggtgc cctccgatgt gccggtcgga ctcttcatac tatggtgaag
    13201 gggatgatag tagaaaaaag cacctcccgg ggcaccctcc gggaggtaaa accttatact
    13261 ccggcgctcc aggggggacg tgagatggcc tatggcgggg aagggtgcat cgatatcggg
    13321 gggagaggct gcggaccatg gcaatgagga gcggctccac cggcacctgg cgatgctcga
    13381 gtccggggat ctcaaccagc gctggcgggc tgcggatgcg ctcggggaga tgggggatcg
    13441 gcgtgcggtg caaccgctga tccgggcgct ctcagacgag ttcgtcgatg tccggtggaa
    13501 ggcggcaagg gcgctcggta tcatcgatga acgcgaaccg gtgctcccgt tgatacggag
    13561 cctggaggac gagagctcct gggtccggga gagtgcggcc tgggcgcttg gaacgatagg
    13621 cgacccccgg gccgtcgagc cgctgatagg ggtgcttgcc gatacaaaac cccgggtccg
    13681 ccgggaggcg gcgcgggcgc tcggccggat aggaatcccg cgggccaggg aaccccttgt
    13741 gcgcctcctc ggcgatcccg accgcggcgt caggcttgcc gcccgggcgg cgctcgacga
    13801 tatcgggacc gagcgggaga tcccgagcgt ttgacgggct gtttcctgct ggtcgccaga
    13861 ctcgcgtggt cgaccgcacc gcctctctct ccgggaggcc ggtaacccgt ttctctcccg
    13921 gatataccct cgatccagaa acgacttccg ctattcttcc cggacattgc gattgattta
    13981 tatatccggc cctccggaaa tctattgaat ggacttattc atgaagtgcg ccatcgagga
    14041 ggcggagatg ggcctgcggg aggggggaat acccatcgga tcggtgctgg tccggggcgg
    14101 ccgcatcatc ggccgggggc acaaccgccg tgtccaggag aacgaccccg tgatccatgc
    14161 cgagatcgac tgcctgagga atgcgggccg gatcggcacc tacgcggatt gcacgctcta
    14221 ctcgacgctg atgccctgct acctctgtgc gggagcggtc gtccagttcg gtatccggaa
    14281 ggtcgtggcc ggggagtcgg ccaactttgc gggagcccgg gagctccttg agtctcacgg
    14341 ggtcgaggtg atcgacctgg atctcgacgt ctgtaagacg atgatgcatg cgttcatcga
    14401 ggcgtacccc gatctctggt acgaggacat cggggagctg tgagcggtcg gggctccgtg
    14461 gcagatacgt tcctccgtta caccgccgta gccgttgcca tgttcgtcgt cggggcctcc
    14521 gtcgcccttc tggccgctcc ccacccggag agcagcgccg accgggagac ggtttttttg
    14581 gtctcgacga tcgatgcgct cctgcagggc gcctacgacg gcagcgtctc gtttgacgaa
    14641 cttgcggcgc acggcgatac cggcatcggg tgcggcgacc gtctcgacgg ggaactgatc
    14701 ggccttgacg gcgagtggta cctgatccgg accgacgggc gggcctaccc ggtcgccggg
    14761 agcgcgacga cgccgtttgc ggccgcgacg ttctttgatc cggatgtagc ggtcgcgatc
    14821 gacgaaccgg tgaacctgac cgctcttctc ggccgcctgg agactatgtt cccctcagag
    14881 aacctctttt acgccgtcag gctggacggc accttcccgc gggtggtgac ccggagcgtg
    14941 cccgcccagg agaaacccta cccgcggctt gccgacgtgg tggcaaacca gacggtcttt
    15001 gagttcgaga acgtcaccgg gaccgcggtg gggttctgga cgcccgcgct ggcgaaaggg
    15061 atcaacgtcc ccgggtacca cctccacttc atcacgagcg accgcaccgc cggcgggcac
    15121 ctgctcgata tgaccgtggc agagggcagc gtgcagcttg acaccacggc aaacttcacg
    15181 atggttcttc cttcccgcgg cgatttcctg aaggtcgacc tctcaggcga cctctcgggc
    15241 gaccttgagc gggtggagaa gtagcgcgcc cgagggcagg gcgctgccct ccgccccccc
    15301 ggggggccgg cgggaagccg tgcgcctatc cggagcgcgg acgcaaccct gccacgggtg
    15361 ggccgaaaag agatgtttac cggacgatga gcagcggcgg cctgatcacg ccaccgatat
    15421 cctcgatcag ggcgcctatc cgggccttcc cgctcttgcc gaaggctccc atgacgacca
    15481 gcccgtatcc gccggagttc gcctcgctga cgacctcctc cctgacgcga ccctcgacga
    15541 tctttgacga acaccggata ttctcctccc gggagcgcga gaggatgccg gcgatgtcac
    15601 cctcggtgct cttgcgcata tccgcccaga tccgaccctc ataatcctgc agccggtcgc
    15661 atatgccgct ctgagtacag aagttgaacg ccatcgcctt ggcctccttg atatccagca
    15721 ccgagaagag gaccacttcg ccattcttct gctttgccac ctcaattgca tgcagagccg
    15781 ctttctggct ccacttcgaa ccatcgatca gcacgagaat cttcataatg ttcacatccc
    15841 aaatgtcagc cagagcatcc cgaacccgac tcccaccgtc acgaagagca ccagcatccc
    15901 caccttcagg aaatcgatga aggtgatgcc gatcccttcg cgctcggcga taccgatgac
    15961 gacgacgttt gccgacgccc cgatggcggt accgttcccg ccgaggcagg cgccgagggc
    16021 gagcgaccac cagagcgggt agacatccat cgtcgtcccg aggtcgtgta tgagcggtat
    16081 catcgccgcg gtcaggggaa tgttgtccac gatcgccgag gcgatcgcgg caaaccaggt
    16141 cacgatgaac atcgcctcgc caaccgatcc gacgttctcg atcatgaccg cggcgatgct
    16201 tgcgatgata cccgtctcga cgagggcgcc gacgatgacg aagagcccgc cgaagaagaa
    16261 gagggccggc cactcgatct tctcaaatat ctcctccggc gactgccggc tccagaagag
    16321 gaggaccgcc gccccgatga gggccacctc cgcaggctca agccccatcg ccgggtcgac
    16381 aaacggcagg acgacgtgca ggatctcccc gatacggtcg tgaataaaga agaggaggac
    16441 cacaaacgcg atgacgatca ccgactgccg gaagagcacc cggtcggtga tagccgcccg
    16501 ttcatcgagg ctatcgagcg tccggaccat ctcctcccgc tcgccgggac tcaccttcat
    16561 ggatcggccg tatatgacgt acatcatcag gaggaggatc accatatcga cggccatgat
    16621 ggggccaaga tggatcagga actcgttgaa cgtcagtccc gcagacgacg cgatcatgat
    16681 gttcgggggg tcgccgatga gcgtcgccgc accgccgatg ttcgaagcaa atatctccgt
    16741 gaccaggaac gggacagggt tgagtttcat cacccttgcg acgtagagga gcatcggtgt
    16801 gaggaggagg accgtcgtca cgttgtcgag gaacgcgctc accaccgcgg tcacgagcgc
    16861 aaagagaatg aggatccgga tcgggctccc gttcgcgagt ttcgccgttc gtatggctat
    16921 gtactcaaag agaccgctcc cccgggcggt gttgacgatg atcatcatgc caaggagcag
    16981 gaagatcgtc ccgaagtcga catgctcaag gagcccctcc caggggacga ccccgatgaa
    17041 gacgacgatc gccgccccga acatggcggc aaccgcccgg tggatccgct catcgatgat
    17101 gagcgcgtag gtgaagagga agaccgctac ggcgatcagc gcgagaacat ccatcgatgc
    17161 acccgtcagt agatgagcgc cggggtctcc acccgctggc agatgcgcag gacgatcggg
    17221 ctgaccgacg tctcggtcga ctcgccgtcg gcatagcgcc ggcagagcgc gaccatgtcg
    17281 tgattcttcg ccatccggac gacgtcgtcg cctttatgtc cgacaaacat ccgtgtctgg
    17341 accataaaac catggtcacc gagttttgcc atggtccggt cgaggagttc ccggccgtac
    17401 tcttccttct ttgcccggaa ctcttccgtc gatgcatggt tgagcgcctg ttcgaggagt
    17461 tcgatcaccc cggcgtcggt gatgtaggcg agcgttatgg tggcgttata agcagagagc
    17521 acatcgatcg cttcgtcggt gatatcgtgg acgaagaggt cgagcgggat caggatcgat
    17581 cggacttccg ggacgcgcat ctctttatcg gtcagcagaa attcgctgta atccttgagg
    17641 accgcttcgt agcgccttcc ggcgatatct ttgaactttc ttcggatcaa ggaagatgag
    17701 tatcccatgg tgattcactt ctacgttttc cagagcttcc gtttaatact ttgcccgtcc
    17761 gaccctgccg gacacccctg gagaacaccg cgatgcaacc cggagcggtt tccctcaccg
    17821 ggagcctgcc gcctccctgc aggccggaca gagcatcttc ctcttccggt ccagttcatc
    17881 cagcgtctgc ggacggaaca tgacgcactc ggggtcggcg cagtgcccga ggccgaggag
    17941 atgcccgatc tcgtgcgctc cctcttttgc cgtccggtcg atcaggtcgg cgtcgtcggg
    18001 cgtcctgccg tagtagccgt tcccgaggcg ggcggtcgag acgacggcga cgccgcttgc
    18061 cggccgtgcg aggccgaaga cgaagtcgca cccgttaata tagaggtcgc gggaggtaac
    18121 gaggagcagg gggccggcaa cctcgtacct gcgggtgagg gtatcctgca ggcggtcaag
    18181 gatcttttga gcatcgtgct ggttgcggtt cctgtcgtag ccgtcgatga ggaacggata
    18241 ctcgacgaat ccgatctctc ttccaaggat catcgtcatc atccgggcga ccgggagctc
    18301 gaagccttcc ggggcctgcc ggtcccaaag aatattgatg cccattgcct aatctctggg
    18361 taatgcgtca ctataaacgt attgaacggg gtgtcggggg gtatatcgcc ggccgctacc
    18421 ggtgcgcagt ggaggtgggc ataggcaaca accccgatgc cgcccgcgtg atcgcggccg
    18481 ccggcggcct tctgctctgc accgacatcc ggcccggcat ccgccacgac gggctcaccg
    18541 tggtgaccga cgatatattc gagcctgacg cgaggttata cgcgggcgcg gacctgctat
    18601 acgccgtgcg ccccggcgtg gagatggtcc cgccgctgat cgcgcttgcc gcccggatca
    18661 attgcgacct cctggtctac cacctggggt gcgagatata cggggacggc ggcgatgtcg
    18721 tggactgcgg cccggccctc ctccaccgct accaccgccg ccgctcttaa aagagggttg
    18781 cctggccctg cgggaggtcc gggcgcgcct ctccgggcgg gtcgtcgccc cgggtctttc
    18841 tggcaggcgc tttctccttg ccggccgctt tctccttacc gcccgccttt ttctcttttg
    18901 ccttctcctc ttttgccagc tctttgacga tctttgcggc gcgggctttg tcgtggatga
    18961 agaggttcag ctcgtcggcg tcgagctcga actcgcggac gtaacttttt ggatcctgct
    19021 cgacgaggat gccgatcgcg gtgaagaggt cctcgcggag ggcggcctcc ggtatatgcg
    19081 ccatattgct gagttttcgc gtcagacccg cccgcaccgc cttctgtttc tttgatgccc
    19141 ccatcttctg ccagcgcgac gggggcatga tgcggccgcg gacgccgcgc ccgccggcgg
    19201 cgtcggcaac gccaaggagc atgaccgcgc ttgcgtaccg ccagagggtg tagtactgcc
    19261 gccggaacgt ccggccgata tacccgtccg cccgcgcgag gcaggcgtag cccttctccc
    19321 gggaggcgag atcggggatg taatcgatgt tgccctcgag ccactgcacg atggtgtcgg
    19381 gcgtatcttc gacttccatc gccgtacgga gcagatcggc atcgtcctgg cccttaaaga
    19441 ccgcgccgac gagctcgaat atggtggagc gttcgtcctt tgcggatgtg tggacgtcgc
    19501 ccgccgcgag gctttccttc ccgatggccg cggcgtagag catgttcacc gccgcccgca
    19561 tatcgccgcc ggcgcgctca gcgataccgt cgagtgcggc ggggtcgcac gttaccccct
    19621 ctgcggtgca tatgtggcgg aggcggggga cgatcgaacg ggcctggagc gcccggaact
    19681 ggaccggttc cgtggccgct ttgaggtcgc gcgagaggga gtagtagtcg tttgctatca
    19741 ggatgatcgg ttgctgtgac gccgcgatga tctccacgat cgccttcgcc ccgccccggt
    19801 cggcctgccc gtgcaggttg tcggcctcgt cgagcaggat gagctttcgg ctcgcgccga
    19861 ggaggctggc cgtggtcgat cccgaacctg ccacccgctc gagggccgac ttcgtccgct
    19921 ggtcggaggc gttcagttcc accacctccc agttcatgtc gtttgcaagg gcgtacgcgc
    19981 ttgaggtctt gccgataccg ggtttcccgt acaggatgag tggtttcttc tgccgcgacc
    20041 agtcccgcgc ccattcgtac atctgcctga cggcgccggc gttccccacc acatcccgga
    20101 ggcgctgcgg cctgtacttc tccgcccagt ccatggggat ctatacgacg cggtgcatca
    20161 aaaataccac cccggagggc agagcgactc tggagcaacg tggcgatgga gcggcagggc
    20221 aaccgcaggg ggccttctcc cgatctaaac cgggcgggcc gtgctgccgg ttatcggctt
    20281 ccggagcaga gttcataata caggtgaatc aaatacatta tctctgatga gacagaatac
    20341 catacagaaa ggtggagtgt tggtgttctc gtggtgaaag attgctccac agatacggtg
    20401 gcacaggccg tcagggaata tgaaggcgtt acccggaaga gagcgatcgg ggatatggtc
    20461 cggtccctcc ggatcgagaa ccccgatatc gtcgcctcgt tcggcgagga tgccgcggtc
    20521 atccggcata acggagcgga tgcgttgctg cttgcggcag acggcatctg gagcaagctc
    20581 atggaggcgg acccgttctg ggcaggatat tgtgccgtgc tcgtgaacgt tcacgatatc
    20641 gccgctatgg gcgggagacc ggttgccatg gtcgatatcc tctctgttac aaacgacgcg
    20701 atccgcgata acgtgacccg gggcatggtc gccgcatccg cccagttcgg cgtcccgatc
    20761 gtgggcggac acctgcaccc cgagacgccg tacagcgtcg tggatgtggc gatcctcggg
    20821 acggcccgga tggatcacct catcttctcg gatacggcag aggtgggcga ccgtgtggtc
    20881 gcggcgatcg acctcgacgg ccgggttcac ccgtcgtgct gcttcaactg ggactccgtc
    20941 acgatgaagt cggcggaagc ggtgcgggcc cagatccggg tgatggagga cctcgggaga
    21001 gatcgcctgg tgacggccgg caaggatatc agcaacccgg gtgtcatcgg aacgctcggg
    21061 atgctccttg agatgagcgg gaagggtgcg gtggtcgacc ttgaggcgat accccggccg
    21121 gacctctccg cgctcgatct ctccttcgag cagtgggtgc gcatgtaccc cggcatgggg
    21181 ttcatactga ccgtgaagga ggagaacgcg gaggaggtct gccgccggtt taccgatgtc
    21241 ggcatcaccg cttctctcat cggagaggtg aacgggagcc ggaagctgac catcaggtac
    21301 ctgggccggg agacgcaggt ctttgacctg gaccgggacg gcatcatgca gatcttctct
    21361 gaaggggatg tatgccggta acggtcggac ttggtgcggc atcccccgat gagaagatcc
    21421 gtgcgaccgt ccgggcggtc ggccgggagg tcgatctcac tcttttttcc tgtcccggaa
    21481 ccgccggttc gttcggagac gccgtcaggg tcgtcgaggc ggtacatccg gaggctgccc
    21541 ttgtcgaggc tcttgccgcc ggcgatatcg atgccgccgt caggggcact ctgccggcgt
    21601 cttccacgct tgcggccctc aaggcggccg ccggcgtcga ccacctggag cggatagcgc
    21661 tcctcgagac cgccgaaggg cacctcttcc tccttgcccc ggtcggtgtc gacgagggct
    21721 ggacggtcgc ggacaaggtc cggttcgtcg agcacggccg ccatatcgcc cggagtttcg
    21781 ggcttgccgg gggggtcgcg gtcctctcgg gcgggaggct cggggatatc gggcggcacc
    21841 cggccgtcga ccggacgatg gcggacgccg agctcgtcgc ccgcctgacc ggggcggagc
    21901 atgtcgagat cctgatcgag gaggcgacga ggcgttacgg ggtggtcatc gctccggacg
    21961 ggatatcggg gaacctgatc tttcgtacgc ttgcgttcct tggagccggg gctggacacg
    22021 gtgccccggt ggtaaatatc gataaaattt tcgtggatac gtcgcgcgcc tcaccagatt
    22081 atacgaatgc tataatgctc gcgaaatcgt tagcggaatc cagaaaaccc tgaataatac
    22141 tcccttttct ggaaatatcc gtaatccggg gttatttgaa tcgtcacaat tttacgccgg
    22201 gtatcaggat gctctgccca taagcgacgc cgataagaaa gagaaacgca aattttcgaa
    22261 ataatcccga aatgtttaaa tattaactcg tacacctctt tttagaggtg aaatttgatt
    22321 atggcagatt tacctattgc tgcggtcgtt cggatcgcaa agaaaaatgg tgctgagagg
    22381 gtcggcagcg atgccgctgc tgcactcgtc ctgaaggctg aggcgtatat cgccgagctg
    22441 accaaggaag ccaacaggct tgcccagcac gcggggcgca agacgatcaa ggctgaggat
    22501 gtcgagctcg cggtcaagtc cgcgtaaccc ggctgctcac tctctctttc tctcttctcc
    22561 ttaccggggg tccgagagac ccccttctca aggcccgtcc cggcggtgat ggtctctctc
    22621 cggaggggcc ggcgttttgt ggatatctcg tcgtgacgca taccgggcca tgcccatgct
    22681 ggcaatgggc gtcagacgct tctatcttcg atcccaggca ggttgtgacg ggagacgggt
    22741 agaaatagat gccgcggaca aagagaggct ctgaaggccg atacgttgcc ccccggggca
    22801 acgttctctg ccggcatctc tgagggggga aaaccgatgc gttcttcgcc ggcatccccc
    22861 acccgcgcct tcggcagggg gatacccgtg catttctccg gcaggagata cccgcacact
    22921 ccttcggcct cccccacccc acccgcgcct ctgtaggaag atgcccatgc gcttctgccg
    22981 gcatcccccc cgccggcgcc ttcgtaggga tacccagaca cttccccggc acccccaccc
    23041 cgcccgcgcc ttcggcaggg atacccatgc acttctgccg gcatcc
//
LOCUS       DAOSII010000016        22213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_2592_length_22213_cov_50.829497, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000016 DAOSII010000000
VERSION     DAOSII010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 22213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22213
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2592_length_22213_cov_50.829497"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /gene="ftsZ"
                     /locus_tag="PLV90_02370"
     CDS             complement(<1..686)
                     /gene="ftsZ"
                     /locus_tag="PLV90_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOF95990.1"
                     /translation="MKSIVEEALSRAREEQRFAEPTGVDKDIEDVLMELRTEIAVVGC
                     GGGGSNTITRMSEEGINGARLIALNTDAQHLIRTHAETRILIGRQRTRGLGAGSIPQV
                     GEEAALENEDDIKRAVEGCDMVFITTGLGGGTGTGSAPVVAKAAREEGALTIAVVTLP
                     FTAEGAIRAQNAEAGLERLREVADTVIVVPNDRLLEVVPRLPLHAAFKVSDEVLMRAV
                     KGITELITMPG"
     gene            complement(683..1996)
                     /locus_tag="PLV90_02375"
     CDS             complement(683..1996)
                     /locus_tag="PLV90_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048063716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOF95991.1"
                     /translation="MRIALINSRVDAAGVNIRDHLGVLLTEGGRWPLADDHELVFHEV
                     AGRLIYQDRIDEEVDADLIVFISRHASAQPVPALTVHVTGNYEAADLGGEPGALAPAA
                     PAWMHAILGNLAARAPEGYRVSYEVTHHGPTALATPSLFVEIGSTAAEWADPAAGRAV
                     AESILAAAPQETIDLIGFGGTHYAVRQTAIALASRGAFGHIAPTRQIGALDLDLVRRM
                     REASRAVAAYIDKKSLSADEAARVERLLDGAGIPLLTESEIREIGDLEWATYLRIRAL
                     ADEIAPGSRARIHGLAGQGTPVPVDVNRDLVEETAKSDKTGFITALDGLPVAHLSKGS
                     TEVLPTFIGFVYGSSRLASDITTLCVKLLLISEDAVIDGDHLVLRKVRFDPEKARRLG
                     VPRGPLFAMLAGGKAVEIGGQTVTPDAVQATSIKRIHIPGLERYI"
     gene            2089..2271
                     /locus_tag="PLV90_02380"
     CDS             2089..2271
                     /locus_tag="PLV90_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF95992.1"
                     /translation="MDEEKVRKAFEDYGITDEITCPQAFDISEKYNIPKMDIARYCNQ
                     REPRIKIRSCQLGCFR"
     gene            2268..3470
                     /locus_tag="PLV90_02385"
     CDS             2268..3470
                     /locus_tag="PLV90_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOF95993.1"
                     /translation="MSLFLSVVSVGKAVEVVRGIAPPLESGDVPLENALHRVLAEDIR
                     ADIDIPGFDRSTVDGYAVIATDTTGASEAIPAMLSCRGRVGMGSADAGSITPGSCCYV
                     PTGGALPRGADAAVMIEHAEQIGDDVLVHRSVAPGENVITRGEDFRAGTVVLERGRVL
                     SPRDIGVAAAIGADRVTVAAVPRVGIISTGNEVVPVTATPGPGEVRDANSYLAGAFVT
                     ERGCRPVYFGIVRDEREALKAAVSSALDTCDVVLVSGGSSKDERDNTAAVVADLGEVL
                     VHGVALAPGKPTIIGTARGRPVIGLPGHPASTFVVLVAIVDHLLAAMRGTAVSRRTLS
                     ARLKQNIPSTRGREDYVRVSVRDGEAVPIFGKSGLLNTLVQSDGIVRVPASSEGLEVG
                     EEVEVILW"
     gene            3464..5317
                     /locus_tag="PLV90_02390"
     CDS             3464..5317
                     /locus_tag="PLV90_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HOF95994.1"
                     /translation="MVKRYLSVISLAEARALVERSFPAIPGVVRVPVTAGTVGRITAA
                     PIFARFSVPAIHLSAMDGIAVRSAETLGASEQNPVTLPDAARVNTGNIVPAGYDAVVM
                     IEDVWVDGETYTVRKPVSPWQHIRPVGEDIGESEMILSSHHRIRPQDVGALANYGVTE
                     VDVLNVRAGLIPTGSELVPAGVMPPPGKVVESNTLMAASVLAEAGVEAHRYPIVPDDP
                     DRIRDAVRRGVAENDILLVSAGSSAGTRDYTADIVRDLGEVLAHGVAIKPGKPVIVGR
                     VEGKPVIGLPGYPLAAATVLRELVMPLVARYGLPVREPGHVAARLTTSLQSDIGTDEF
                     VLLSVGKIGDRWVAVPQSRGAGIQMSAVRANGYLRIPEDMEGAEAGKTVDVRLTVPRA
                     EAEEAVLVTGSHDPALDYLADLVRPRGVDIHSTHLGSMGGVIALKKEECHAAPMHLLA
                     PDGTYNTYYLERYMPGADLALLCVAERQQGVISRDGLSFDDLPGRTFINRQKGSGTRM
                     LLDHTLAARGIDPASIPGYGREATTHLAVALAVRTGEADAGMGVYSAAKALDLAFVPV
                     ATERYELAMHRAMLDDPGIAALVETVSSAAFKQVLRDLGGYETDETGVLRG"
     gene            complement(5253..5726)
                     /locus_tag="PLV90_02395"
     CDS             complement(5253..5726)
                     /locus_tag="PLV90_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HOF95995.1"
                     /translation="MQIAVVGRGDCSGEEYAAARAVGRLIAGIGAVVCCGGLGGVMEA
                     ACRGAREAGGTTVGILPDTGDGNPYLDVAIRTGMGHARNVVLVHSADAVIAVGGGYGT
                     LSEIAFALKTGKPVYGLATWEIEGVVACATPEEAVSRAGRPSRPSRSPRGPGGPA"
     gene            5842..9051
                     /gene="ileS"
                     /locus_tag="PLV90_02400"
     CDS             5842..9051
                     /gene="ileS"
                     /locus_tag="PLV90_02400"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOF95996.1"
                     /translation="MKEVTSSYNPRDIEAGVQDYWTKENTYARVRALREGGKAFFFVD
                     GPPYTTGHIHLGTAWNKIIKDTILRYHRMRGADVIERAGYDMHGLPIEVKVEHQLGFT
                     SKKDIEEYGIAAFVEQCRTFAVTHMGIMSDQFRRLGVWLDFDNPYQTIKEDYIESAWW
                     TLQRADERGLLERGHRVVNWCPRCETAIADSEVEYWDETDPSIFVKFPLSGRENEYLV
                     IWTTTPWTLPANVAVAVNPAFIYARVKAKKDDVEEILWIADELIEPVLKMGRYQDYTV
                     LERVSGSDLVGTEYTSPLAGCVPHQAEIRHRVVAADYVALENTGLVHIAPGHGWDDYL
                     IGIREGLEAFCPVDSGGCFTPAAGAFADMYVRDANEIVIDALGGYLLARRTITHRYGH
                     CWRCKTPIIYRATAQWFLKATAIRDSMLEEIAGVKWYPDWAGSARFHDFVKESRDWCI
                     SRQRYWGIPIPIWHCEHCGEYTVVGTIAELEERSGAKVPDPHRPYVDEVVIPCPCGGE
                     MRRVEDIFDVWFDSAVASWATLGFPREREAFDRLWPADFITEGQDQTRGWFYSQLGAS
                     NVAFARAPYKSVLMHGFALDAEGRKMSKSFGNVVTPEEVVNQFGVDVLRFYVLWANAP
                     WDDLKFNWDGVKTIHRTLNILWNVYRFPLPYMILDSFQPAAGEGERWDDSFVRSHIRD
                     MPEEDRWIVSRANTLIRAIGEDMQEYHLHRETRALAAFILEDLSRWYVQLVRPRMWLE
                     EDSPEKRYAYETFYYVMRRLITLLAPFTPYIVEEIYQNLRLAGDPESVHMLDWPEADD
                     LLIDPALEADMGVIQSFDDAVATARQAGKRKLRWPVSETVVVTGSDEVKTALQDLNDL
                     ARTRANARAVRVVTGTWERILWQAAPVMRAIGPEFGREGPKVKALIEGADGTALKAAI
                     EREGKAELDGYEITARHVAFTEALPAGVFAAPMKDATVYVDVALTPALEAEGYAREVI
                     RRIQEMRRQLDLNVDDYIVADVHVADGRIVALIGEEEWKDEIAGEVRAAVLTVRHADG
                     ERPAEPFALEKDWDVEGVQMRIGISRADKRPDKGRPLDR"
     gene            complement(9004..9738)
                     /locus_tag="PLV90_02405"
     CDS             complement(9004..9738)
                     /locus_tag="PLV90_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008193 - tRNA guanylyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(His) guanylyltransferase Thg1 family
                     protein"
                     /protein_id="HOF95997.1"
                     /translation="MDINDREIFSRLAIFPPFFVRLDGRAFHRLARALELTRPFDPRF
                     HAAMRSACRYLLTGSGLTPLFAYTFSDEISLYFSTLPFSGRVEKIDSVTAASAASALT
                     IELGCREPLAFDARVIPAAGEFAVEYLVSRQNEAWRNHINAYCQSALVDEGMSPRDAA
                     AALRGMRSEAMHEIMFERGVNLAATPAWQRRGTLLYREECIKEGYNPLTGERVEATRT
                     CIREPEETPLFSTVEGTAFIRSLIGA"
     gene            complement(9766..10029)
                     /locus_tag="PLV90_02410"
     CDS             complement(9766..10029)
                     /locus_tag="PLV90_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOF95998.1"
                     /translation="MRTEITNLFELPVYTEKAIFVGNVDDIVVDVDQKKIDSLAVGNL
                     DPAIREVMKGSGLKIPFRIIKSIGNIVIIRHIPGLFRSPAKEE"
     gene            10083..11219
                     /locus_tag="PLV90_02415"
     CDS             10083..11219
                     /locus_tag="PLV90_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOF95999.1"
                     /translation="MNASLQIGKLAGIPVKLHWSFLLVIPLFAWIIGSQIRLTTELIA
                     TLFGVPIDVTLIAAGLNPYILGSIVALGLFVGVFIHEMAHSLIAKAKGIEIHSITLLI
                     LGGVSQMEETMPDPKIELPMALAGPMTSLAIGLISAALVYVFDAAVADPAVAGVLVFT
                     FGYLGLLNILLFGFNLLPAFPMDGGRVLRAWLARRMPLSRATRIAADVGKGFAVVFGI
                     VGLLFLNPILIIIAFFIYIGASQEATVLRYNILLQDITVADAMSSPVATVEPTLPLSR
                     LVEMMYETKHLGFPVVDRGALVGIVALADIHKITPQDREAMQVRDVMTRNPTTLPPSA
                     PLLDALKIITGKDIGRIPVVSDGTLVGIVTRTDVLRVMELREED"
     gene            complement(11216..12853)
                     /locus_tag="PLV90_02420"
     CDS             complement(11216..12853)
                     /locus_tag="PLV90_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOF96000.1"
                     /translation="MTSNALIIDGYVDEPACLGVAPYISPYIREVAGVLVEHGYTTGY
                     VTIDQVRADPSLVAGHSDLVVMIAGLTVPGAYIGGKPATLTEIQQLGTLLREPATAIG
                     GPIAFGYAPGGGRKAIRQAIAGFDATLSGSPAVALDAWLSGGEPAGAGDYSLTDPWAV
                     AGAGIVARHPAFPYVMCELETATGCSRATVGGCSFCTEPFYGLPRYRSIEGIAREVAA
                     LHAAGARHFRLGRQPDLLAYQSHGGEFPAPRPEVLAELFSAVRESAPGLATLHVDNIN
                     PGTIAHHEDAAREALGAIVAGHTPGDTAAFGMETADPAVVAANNLKAMPDDVFRAIEV
                     TNDVGGKRTKGIPELLPGLNFVVGLAGETPATFDANEAFLRRVLDAGLLVRRVNIRQV
                     MPFEGTAAYEKNTLGAHDARFRAFKEWTRREFDEPMLRRVFPIGTVLRDVVVEIAGRP
                     SFGRQMGSYPILAGIPLDLPAGTVLDAVVVDWGMRSVTALPCPVEVNTLPVAALRWIP
                     GVGKKRAATIAARRPFENIAEFQKIVGETGIEGAMKF"
     gene            complement(12843..13466)
                     /locus_tag="PLV90_02425"
     CDS             complement(12843..13466)
                     /locus_tag="PLV90_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOF96001.1"
                     /translation="MRFADWEPHYTAILDYFGFERDADEAAARLLEEVADGRDDVGLL
                     EALIRGRAVTVCGNAPSLPDELDRIEGTVLAADAAAEVLAGHGIRPDAVFTDLDGATD
                     VFVDLSRQGTVMVVHAHGDNMPLIRHWVPRLPGPLVATTQAAPFAHIHNFGGFTDGDR
                     AVFAADDLGAASIRIIGFDLADKNVDPVKRGKLYWAGELLRLIGYDL"
     gene            complement(13463..14926)
                     /locus_tag="PLV90_02430"
     CDS             complement(13463..14926)
                     /locus_tag="PLV90_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOF96002.1"
                     /translation="MPEAVQNVSKERIKYIIFGCGSTGYNVAEELGQESEDLIIVDKD
                     EKRVEDLRDQKYEALVRDLRDPNLMEGLPVPEVAFILTNDRDANLTALKTIKNRYPAT
                     YVIARATDPVSVDLLQQEGADIVLYPQEVVARTAIHHIRKLHSSRLALRLYDLLAAWE
                     GTLCIVTHLNPDPDSISSAMALSMIARHASHNKLNCRILYEGDIGHQENRAFINLLEI
                     KMERLTPQILSECNYIALVDSSAPGANNELPKTTRVNIIVDHHKNGERPPGAADFVDI
                     RPGVGATASIMTQYLMELDIPVSKSVATALLYGIRADTRDFKRNVTPQDLNYAAFLLP
                     LTDSDLLDKITSPSISQETVEIMGNAIRNRRIRSGYLFSNVGFIRNRDALPQAADILI
                     HLEGVNTALVYGISDQNIVISGRNKDIRLHLGNVMAEAFGPIGEAGGHATMAAAMIPL
                     SYFSMVKEKEELLNLIIDPILKRFANIVGLDNNEGKQ"
     gene            15040..15112
                     /locus_tag="PLV90_02435"
     tRNA            15040..15112
                     /locus_tag="PLV90_02435"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15073..15075,aa:Lys,seq:ctt)
     gene            complement(15475..16362)
                     /locus_tag="PLV90_02440"
     CDS             complement(15475..16362)
                     /locus_tag="PLV90_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF96003.1"
                     /translation="MITARSLAKHYGDFPALDDVTFDLDDARILGVIGHNGAGKTTLL
                     KIMAGLVTPTAGNLLIDGIDVVRRPFNLKRNLGYLPEESRLYETMTTDTYLAFFGEIY
                     GMPKAAIRTRRDDLLAGLALEPDGKKIGELSKGMKRKVAIARSLMHDPGLLIYDEPTS
                     GLDPMTSRSVLEYIKGLRDRGKTVIFSAHNLSQVEEVCDRVLILRRGRVVAGGTIAEL
                     RETFGSITYRVFFRIPDLSSFAPPVEYSAHDGRYLAEARSIDELNRTTAALAAEGAGI
                     DRIESHYPTLEEMLLKIGR"
     gene            16442..18247
                     /locus_tag="PLV90_02445"
     CDS             16442..18247
                     /locus_tag="PLV90_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HOF96004.1"
                     /translation="MSLASSIRTIAVWEMNRSMTTMGRGVLPLAAGLLVLLVLVTAFA
                     AESGIHMQDGIYRIGIDDPDVARIVDADTRFTVETVSGPALWNNRFAYDIVVIRGEVY
                     AADTGKGRAALTALKRDYEGYVSRIAAGEPDLFAGYPLWIDLEYVKSEIDFLATQSGQ
                     QVGAPANAREPPVPSGPVEAVTLPPSTVPVHEEDLRQHLAGMEKTNPLSRYTGIVSSG
                     SATDGLRTPSELSPPLPYDALILVFVFIFPLYFTSQFFMMSVMNERVGRAGEALLAAP
                     VRPAAIIVGKAIPYFAIMLLVSTAITFFIGAPLTILLPLIPVILFFLSSALIIGMAAR
                     SFKELSFVSIFFSTLATSYLFFPTVFANTHVVSLISPLTLVILELQGEGFTAAGYVYS
                     TALFFATSAVLFYAGTINFREERLFSEKSLTSRLMDFVAGAISRDRPHFSLFLLAAFT
                     IPFVFMAQMMLLVLFFNIPMPLSLVLLTVSAAFIEEVAKSVGLYAVAGERPGFLTPKN
                     LLVGAAAIGFGFLAGEKLLLFATLSQITESIFGSVLFLSLQVLWMPLLLHIAGVLITG
                     TFLLAGGKRAYVPGLVVASVVHSLYNLHFLLGGLV"
     gene            18244..19329
                     /locus_tag="PLV90_02450"
     CDS             18244..19329
                     /locus_tag="PLV90_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF96005.1"
                     /translation="MNARHIGIVAKKELLGLSAEKTIVFAILLQVFIAMFSSFLMVGL
                     TAMYDPEALEGYSTATYGVGYVGADSALIDEFEKRDDLILHRMDLSPALAALRDRKIA
                     AVVYVPDTPPDAEGPVAITLYLVQNDLQSSVINVKIKEVLQGYERELREVRADRMTAF
                     PVGLNFPDAGDAESFYEFVYGLLIPLLVLLPAIVSAALIIDLITEEYQRQTLDTLLST
                     PVTFTEMVAGKIAACFAIVPVQAGAWILLLELNGIAVANAVPILLHVSAAGLFLILLG
                     AAIALHYRERTSAQFVFSTGLVVVFLLAMAIPYNPLNIVVRLAVGTAGPVHWLILLLV
                     SGATALLAFAVAAYGRRVGKISPTAVR"
     gene            19407..20102
                     /locus_tag="PLV90_02455"
     CDS             19407..20102
                     /locus_tag="PLV90_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96006.1"
                     /translation="MQFSPSMTRLRYLTTTCAVCGESCRFTIPPGAVSAGSRDLDTRP
                     AEPLRSTIYAWVKRCPSCGYCAPDPGKALAGAADAVRLPRYRWQLDARGFPDIANTFL
                     CWSIIQEDLGAPAQAAWASLHAAWVCDDEGEDVPARVCRRRAADLLQRAREQDERLAP
                     LEGADEALLVDLLRRAGRFREARAVAARVLDQDPQPLIAGIMRLQVRLIAASDRGAHT
                     VAEARRSRQPTPL"
     gene            20160..21473
                     /locus_tag="PLV90_02460"
     CDS             20160..21473
                     /locus_tag="PLV90_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-coenzyme M reductase glutamine
                     C-methyltransferase"
                     /protein_id="HOF96007.1"
                     /translation="MDIAIFSPGIYTYGAMLIGGVLRDAGHRVSLTRAPAVPADSLLL
                     ASLFSTQHLLDPAIRDLVRTHREKGGKVYVGGPVSAAPEIVLGELAPDAVVVGEGEET
                     VVRLVAEGVSAALPGIAYLDGGRPVVNPPAPPASIDRPLPLIPGDIGSQSIRGASAYI
                     ETHRGCIGGCTFCQVPRFFGRKIRSRELASIIEEVRAFSAAGATRLSVSGGTGSLYGS
                     ADGEMNPEAFIALLHGMAEVMGARNVSSPDIRVDCITDEILDAVRRYTIGWVFFGIES
                     GSARVLRLMGKGATVRQVEEAVGRCREHGLRVAGSFIVGYPGETERDYEATKDLIARL
                     ALDDVFVSGAEPIPGTPLADLAVRTPRDKNPAFVPHTGEYRPLNLTESEARCFDLMMH
                     ADMFRPQMRLVTDEVYATYLGEARKQGQDIRAATELILRYSPSGA"
     gene            21600..>22213
                     /locus_tag="PLV90_02465"
     CDS             21600..>22213
                     /locus_tag="PLV90_02465"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOF96008.1"
                     /translation="MAEREAGQVLATRPEPGERIDLMRARDVADIKVQNPEGENIGDI
                     TDIAIDRDGGCIAYAVLAYGGILGFGEKHFAIPWEAVRVRSREKIAIVDTDKKTLDNA
                     SGFTRDKMPREGDWSVIRTPPRPTAAKERPAAEGERPPPKEEKPAAPAEPAPRTVESY
                     TPVPPPVQTVAGGWGEKAMKPRTEEQPARAAGAVVEEVRREEPAR"
BASE COUNT         3781 a         7333 c         7224 g         3875 t
ORIGIN      
        1 ccgggcatcg tgatcagctc ggtgatgccc ttgaccgccc gcatcagcac ctcgtcggag
       61 accttgaagg cggcgtggag ggggaggcgg ggcacgacct cgagcaggcg atcgtttggg
      121 acgacgatga cggtatccgc cacctcgcgg agccgctcaa ggcccgcctc ggcgttctgt
      181 gcccggatgg cgccttccgc cgtgaacggg agggtgacca cggcgatggt cagggctccc
      241 tcttcccggg cagccttcgc gaccaccggg gcggagcccg tgccggtgcc ccccccaagg
      301 ccggtggtga tgaagaccat gtcgcacccc tcgacggccc gcttgatgtc gtcctcgttc
      361 tcgagggccg cctcctcgcc gacctggggg atcgaacccg cgccaaggcc ccgcgtcctc
      421 tgccttccga tcaggatccg ggtctctgca tgagtccgga tgaggtgctg ggcatcggtg
      481 ttgagggcga tcaggcgcgc cccgttgata ccctcctccg acatccgggt gatggtgttc
      541 gaaccgccgc ctccgcaccc gacgaccgca atctccgtcc ggagttccat gaggacgtct
      601 tcgatatctt tgtcgactcc cgtgggctca gcaaaacgct gctcctctcg tgcccgtgaa
      661 agtgcctctt ctacgatgga tttcatatgt atctctccaa tcccgggatg tgtatcctct
      721 ttatactggt tgcctgtact gcatcgggcg taaccgtttg tcctccgatc tcaaccgcct
      781 tcccgccggc aagcatggca aagagcggcc cccgtggaac cccgagccta cgcgccttct
      841 cagggtcgaa ccgcaccttc cggaggacga ggtgatcacc gtcgataaca gcgtcttcag
      901 agataagtaa gagttttacg cagagggtag ttatatcact tgcgagtcgg gaagacccgt
      961 acacaaaccc gataaacgtc ggcagcacct ctgttgaacc tttcgataga tgggcgacgg
     1021 gtaacccgtc aagggcggtt ataaacccgg ttttatcgga ttttgccgtc tcctcgacga
     1081 gatctcggtt cacatcgacc ggcacgggcg ttccctgccc ggcaagaccg tggatccggg
     1141 cgcgggatcc gggggcgatc tcatcggcaa gagcccgtat ccggaggtag gtggcccatt
     1201 cgaggtctcc gatctcccgg atctccgact ccgtgaggag ggggataccg gcgccgtcga
     1261 gcagccgctc gacccgggcg gcctcgtcgg cggaaagcga ctttttatcg atgtaggcgg
     1321 cgacggcgcg gcttgcctcc cgcatccgcc ggacaagatc gagatcgagg gcgccgatct
     1381 gccgggtcgg cgcgatgtgg ccgaaggcgc cccgggaggc gagggcgatg gcggtctgcc
     1441 gcacggcgta gtgagtcccg ccaaacccga tgaggtcgat cgtctcctgc ggcgccgcgg
     1501 caaggatgct ctccgccacc gctctcccgg cggcggggtc ggcccactcg gcggcggtgg
     1561 acccgatctc gacgaagagc gagggtgtcg cgagcgccgt cgggccgtgg tgcgtcacct
     1621 cgtaggagac ccggtagccc tccggggccc gggcggcaag gttcccgaga atggcatgca
     1681 tccacgcagg ggccgccggc gcaagcgctc cgggctctcc gccaagatcg gcggcctcat
     1741 agttgcccgt cacgtgcacg gtcagggcgg gcaccggttg cgcgctcgcg tggcgggata
     1801 tgaatacgat gaggtcggcg tcgacctctt cgtcgatccg gtcctggtag atcagccggc
     1861 cggcgacctc gtgaaacacg agctcgtggt catccgcaag cggccaccgc ccgccctccg
     1921 taagtagaac cccaaggtgg tcacggatgt ttacccccgc ggcatctact cttgagttga
     1981 tcagcgcgat ccgcatcagt atacgtgggc cggcagcatg ctaaatgata gtgcttgaca
     2041 tgccctacag taaatcctat ctctttgcag gcagaccatg tgagtactat ggatgaagaa
     2101 aaggtgcgaa aggcattcga agactacggc ataacggatg agatcacctg cccccaggcc
     2161 ttcgacatat cggagaagta caacataccg aagatggata tcgcccggta ctgcaaccag
     2221 cgcgaaccga ggatcaagat ccggagctgt cagctgggct gcttcagatg agcctctttt
     2281 tatcggtcgt ctcggtcggc aaggccgtgg aggtggtgcg agggatcgcg cccccgctcg
     2341 agtccgggga tgtcccgctc gagaacgccc tccaccgggt ccttgcggag gatattcgcg
     2401 ccgatatcga cataccgggg tttgaccggt cgacggtcga cggctacgcg gtcatcgcga
     2461 ccgataccac gggggcctcc gaggctatac cggcgatgct ctcgtgccgg ggccgggtgg
     2521 gaatggggag cgcggatgcc gggagcataa cacccgggtc ctgctgttac gtccccaccg
     2581 gcggcgccct cccccgcggc gccgatgcgg ccgttatgat cgagcacgcc gagcagatcg
     2641 gggacgacgt cctcgtgcac cgctcagtgg cacccggcga gaacgtgatc acccgggggg
     2701 aggacttccg ggcggggacg gtcgtcctcg agcggggccg cgtcctttcg ccccgggata
     2761 tcggtgttgc cgcagcgatc ggcgccgacc gggtcactgt ggcggctgtc ccgcgggtcg
     2821 gcatcatatc cacgggaaac gaggtcgtcc ccgtcaccgc gacgccgggg ccgggcgagg
     2881 tgcgggatgc aaactcctac ctcgccggcg ccttcgtgac ggagaggggg tgtcgcccgg
     2941 tctacttcgg catcgtcagg gatgagcggg aggcgctcaa agcggcagtc tcatcggcgc
     3001 ttgatacgtg cgacgtggtc ctggtatcgg gaggctcctc gaaggacgag cgggacaaca
     3061 ccgccgcggt cgtcgcagac ctcggcgagg tgctggtgca cggggtcgcc ctcgcgcccg
     3121 ggaagcccac catcataggg acggcgcggg gaagaccggt catcgggctc cccggccacc
     3181 cggcctcgac gttcgtcgtc ctcgtcgcca tcgtcgacca cctgctcgcg gcgatgcgcg
     3241 gcaccgcggt ctcccggcgg accctctccg cacggctgaa gcagaacata ccctccacgc
     3301 gcgggcggga ggactacgtt cgggtcagcg tcagggacgg cgaggccgtg ccgatcttcg
     3361 ggaagtccgg cctcctcaac accctggtgc agagcgacgg gatcgtgcgg gtcccggcat
     3421 cgagcgaggg gctcgaggtc ggcgaagagg tggaggtgat cctgtggtga agcgctacct
     3481 ctcggtcata tcgctcgcag aagcccgggc actcgtggaa cgctcatttc cggcgatccc
     3541 gggggtcgtg cgggttccgg tgaccgccgg gacggtgggg aggatcaccg ccgcgcccat
     3601 attcgcccgg ttctcggtcc cggccatcca cctatcggcg atggatggga tcgcggtccg
     3661 gagcgccgag accctcgggg caagcgagca gaacccggtg acccttcccg acgcggcacg
     3721 ggtcaacacg gggaacatcg tcccggccgg ttacgatgcg gtggtgatga tcgaggacgt
     3781 ctgggtcgac ggcgagacct ataccgtccg aaagccggtc agcccctggc agcacatccg
     3841 cccggtcggc gaggatatcg gggagtcgga gatgatcctc tcctcgcacc accggatccg
     3901 gccacaggac gtgggagcgc tcgcaaacta cggggtcacg gaggtcgatg tcctgaacgt
     3961 ccgtgccggc ctcatcccca ccgggagcga actcgtgccg gccggggtca tgccgccgcc
     4021 cggaaaggtg gtggagagca acaccctgat ggccgcatcg gtcctcgcgg aggcgggcgt
     4081 ggaggcgcac cgctacccga tcgtgcccga cgaccccgac cggatccgcg acgccgtccg
     4141 gcgcggcgtc gccgagaacg acatcctcct cgtctcggca gggtcgtccg ccgggacgcg
     4201 ggactacacc gccgatatcg tccgggacct gggagaggtg ctcgcccacg gcgtcgccat
     4261 caagcccgga aaaccggtga tcgtcggccg ggtcgaggga aaaccggtca tcggccttcc
     4321 cggctacccg ctcgcggcgg caacggtcct ccgcgagctg gtcatgccgc tcgtcgcccg
     4381 atacggcctt cccgtccggg aacccgggca cgtcgcggcc cgcctgacca caagcctcca
     4441 gtcggatatc gggaccgacg agttcgtcct cctctcggtg ggaaagatcg gcgaccgctg
     4501 ggtggcggtg ccccagtcac ggggcgcggg tatccagatg agcgcggtgc gggcgaacgg
     4561 ctacctccgg atcccggaag atatggaggg cgccgaggcg ggaaagaccg tggacgtccg
     4621 gctcaccgtc ccgcgcgcag aggcggagga ggcggtgctc gtcaccggca gccacgaccc
     4681 ggccctcgac tacctggccg acctggtccg gccgcgcggc gtcgacatcc actccacgca
     4741 cctcgggagc atgggcgggg tgatcgccct gaagaaggag gagtgccacg cggccccgat
     4801 gcacctgctt gctcccgacg gcacctacaa cacctactac ctggaacgct acatgcccgg
     4861 ggcggacctc gccctcctct gcgtggcaga gcggcagcag ggggtcatat cccgcgacgg
     4921 cctctcgttc gacgacctgc ccggcaggac gttcatcaac cggcagaagg ggtcgggaac
     4981 gaggatgctc ctcgaccaca cccttgcggc gcgcggcatc gacccggcgt ccatccccgg
     5041 ctacgggcgc gaggcgacga cgcaccttgc ggtggccctc gcggtacgga cgggggaggc
     5101 ggatgcggga atgggggttt acagcgccgc aaaagcgctc gatctcgcgt tcgtcccggt
     5161 ggcgacggag cgctacgaac tcgcgatgca ccgcgcgatg ctcgacgacc cagggattgc
     5221 cgctctcgtc gagaccgtct cctcggctgc gttcaagcag gtcctccggg acctcggggg
     5281 ctacgagacg gacgagacgg gcgtcctgcg cggctgaccg cctcctcggg cgtcgcgcag
     5341 gccaccaccc cctctatctc ccaggtggca aggccgtaga ccggttttcc cgtcttgagg
     5401 gcgaatgcaa tctccgagag cgtaccgtag cctcccccga cggcgatcac cgcgtccgcc
     5461 gagtggacga ggacgacgtt cctcgcgtgg cccatgccgg tgcggatcgc cacgtcaagg
     5521 taggggttcc catccccggt atccgggagg atgccgacgg tcgtcccgcc ggcctcccgt
     5581 gcacccctgc aggcggcctc catcaccccg ccaaggccgc cgcagcagac aaccgcaccg
     5641 atgccggcga tgaggcggcc gacggcccgg gccgccgcgt actcctcgcc ggaacagtcc
     5701 cccctcccga cgaccgcgat ctgcatgcag ggaggtaggg ggccgggggt ataaaggctc
     5761 tcgggggtgc gtgagccgca caaatacttt gtccccggag aacaaacggt acaccagagt
     5821 gttggatatg agggatatga ggtgaaagaa gtcaccagca gttataaccc gcgtgacatc
     5881 gaagcaggag tccaggacta ctggacgaaa gagaatacct acgcacgcgt cagggcgtta
     5941 cgagagggtg gaaaagcgtt ctttttcgtc gacgggccgc cgtataccac cggccacatc
     6001 cacctcggca ccgcctggaa caagatcata aaagatacca tcctccgcta ccaccggatg
     6061 cggggcgccg acgtcatcga gcgggccggc tacgacatgc acggcctccc catcgaggtg
     6121 aaggtggagc accagcttgg gttcacctcg aagaaggata tcgaggagta cggcatcgcc
     6181 gcattcgtcg agcagtgccg gacgtttgcc gtgacgcaca tggggatcat gagcgaccag
     6241 ttccggcgtc tcggcgtctg gctcgacttc gacaacccct accagaccat caaggaagac
     6301 tacatcgagt ccgcctggtg gacgctccag cgtgcggacg agcgcgggct cctcgaacgc
     6361 ggccaccgtg tcgtgaactg gtgcccccgg tgcgagacgg cgatcgccga ctccgaggtg
     6421 gagtactggg acgagaccga tccctcgatc ttcgtcaagt tcccgctcag cgggcgcgag
     6481 aacgagtatc tcgtgatctg gacgacgacg ccctggacac tgcccgcgaa cgtggcggtt
     6541 gcggtcaacc ccgcgttcat ctacgcgcgg gtgaaggcaa agaaggacga cgtcgaggag
     6601 atcctctgga tcgccgacga actcatcgaa cccgtcctga agatgggccg ctaccaggac
     6661 tacacggtcc ttgagcgggt gagcgggagc gaccttgtcg ggacggagta tacatcgccg
     6721 ctcgccgggt gcgtgccgca ccaggcggag atccggcacc gggtcgtggc ggccgattac
     6781 gtcgcgctcg agaacaccgg ccttgtccac atcgcgcccg ggcacgggtg ggacgactac
     6841 ctcatcggca tccgggaggg gcttgaggct ttctgcccgg tcgattccgg cggatgcttc
     6901 accccggccg ccggggcgtt cgcggatatg tacgtccggg acgcaaacga gatcgtcatc
     6961 gacgcgctcg gcggctacct cctcgcccgg cggacgatca cccaccgcta cggccactgc
     7021 tggcggtgca agacccccat catataccgg gcgacggcgc agtggttcct gaaggccacc
     7081 gccatccgcg actcgatgct tgaagagatc gcgggggtga agtggtatcc ggactgggcg
     7141 gggagcgccc ggttccacga cttcgtcaaa gagtcgcgtg actggtgcat atcccggcag
     7201 cgctactggg gtatccccat acccatctgg cactgcgagc actgcggcga gtatacggtc
     7261 gtcggcacca tcgccgaact cgaggagcga tcgggcgcaa aggttcccga tccccaccgc
     7321 ccctacgtgg acgaggtcgt cataccctgc ccctgcgggg gagagatgcg ccgggtggaa
     7381 gacatattcg acgtctggtt cgactcggcg gtcgcctcct gggcaaccct cgggttcccc
     7441 cgggagcgcg aggccttcga ccgcctctgg ccggcggact tcatcaccga agggcaggac
     7501 cagacccggg gctggttcta ctcccagctc ggggcaagca acgtggcgtt cgcccgcgcc
     7561 ccctacaaga gcgtcctgat gcacgggttt gctctcgacg ccgaaggacg caagatgagc
     7621 aagagcttcg gcaacgtggt gacccctgaa gaggtggtga accagttcgg cgtcgacgtt
     7681 ctgcggttct acgtcctctg ggcgaacgcc ccctgggacg acctgaagtt caactgggac
     7741 ggcgtgaaga cgatccaccg gacgctcaac atcctctgga acgtctaccg gttcccgctc
     7801 ccgtacatga tactcgattc attccagccc gccgccgggg agggcgagcg gtgggacgac
     7861 tcgttcgtcc ggtcccacat ccgggatatg cccgaagagg accgctggat cgtctcccgg
     7921 gccaacacgc tcatccgggc gatcggggag gatatgcagg agtaccacct tcaccgggag
     7981 acacgggcgc ttgccgcctt catcctcgag gacctctccc gctggtacgt gcagctcgtc
     8041 cggccgcgga tgtggctcga ggaagactcg ccggagaaac ggtatgccta cgagaccttc
     8101 tactacgtca tgcgccgcct gataacgctc ctcgcgccgt ttacccccta tatcgtcgag
     8161 gagatctacc agaatctccg cctcgccggg gaccccgaga gcgtgcatat gctcgactgg
     8221 ccggaagcgg acgatctcct gatcgacccg gccctcgagg ccgatatggg ggtcatccag
     8281 tcgtttgacg atgctgtcgc caccgcccgg caggccggaa aacggaaact ccgctggccg
     8341 gtctccgaga ccgttgtggt caccgggagc gacgaggtga agacggctct ccaggactta
     8401 aacgatcttg cccggacccg ggcaaacgcc cgggcggtca gggtcgtcac ggggacgtgg
     8461 gagcggatcc tctggcaggc cgcaccggtc atgcgggcga tcggcccgga gttcgggaga
     8521 gaagggccga aggtcaaagc gctgatcgag ggggcggacg gcaccgccct gaaggccgcc
     8581 atcgagcggg aggggaaggc cgaactcgac ggctacgaga tcaccgcgcg ccacgtcgcc
     8641 ttcacggagg ccctccccgc gggcgtcttt gccgcgccca tgaaggatgc gacggtctac
     8701 gtggacgtcg ccctcacacc ggccctcgag gccgaagggt atgcccgcga ggtgatccgg
     8761 cggatccagg agatgcgccg ccagctcgac ctgaacgtcg acgactacat cgtcgccgac
     8821 gtccacgtcg ccgacgggcg aatcgtcgcg ctcatcgggg aggaggagtg gaaggacgag
     8881 atagccggcg aggtgcgggc ggcggtcctc accgtccgcc acgccgatgg agagaggccg
     8941 gccgaaccct ttgctctcga gaaggactgg gacgtggaag gcgtgcagat gcggatcggc
     9001 atctcacgcg ccgataagcg accggataaa ggccgtcccc tcgaccgttg agaagagggg
     9061 cgtctcctcc ggttcgcgga tgcaggtcct cgtcgcctcg acgcgctccc ccgtcagggg
     9121 gttatacccc tcttttatgc actcctcccg gtagaggagc gtcccccgcc gctgccaggc
     9181 cggcgtcgcc gccaggttga cgccgcgctc gaacatgatc tcgtgcatcg cctccgaccg
     9241 catcccccgg agagcggcgg cggcatcgcg ggggctcatc ccctcatcga ccagcgcgct
     9301 ctggcagtag gcgttgatgt ggttgcgcca cgcctcgttc tgccgggaga cgaggtactc
     9361 gaccgcaaac tcccctgccg cgggtatgac ccgggcatcg aacgcgaggg gctcgcggca
     9421 cccgagctcg atcgtcagcg cgcttgccgc agatgcggcg gtcacggagt cgatcttctc
     9481 cacccgcccg gagaagggga gcgtcgagaa gtagaggctg atctcgtcgg agaaggtgta
     9541 ggcaaagaga ggcgtaagcc cgctccccgt gaggaggtag cggcaggccg accgcatggc
     9601 cgcgtggaac cgggggtcaa acggccttgt aagctcgagc gcacgggcaa ggcggtgaaa
     9661 ggcccggccg tcgagcctga caaaaaaggg ggggaagatg gcgaggcgtg agaagatctc
     9721 gcgatcgttg atatccatga tcgcccggca ggcaggatcc cgcggttatt cctcttttgc
     9781 cggcgaccga aagagccccg ggatatggcg gatgatgacg atattcccga tgctctttat
     9841 gatgcggaag gggatcttca gccccgaccc cttcatcacc tccctgatcg cagggtcgag
     9901 gttcccgacc gcaagggagt cgatcttttt ttggtccaca tcgaccacga tgtcatcaac
     9961 attcccgaca aagatggcct tttcggtata gacggggagt tcaaacaggt tggtgatctc
    10021 tgttctcatc ggtatcctct gaaggtatac tactataaat gataaaaaga tactcatttc
    10081 ccatgaatgc gtcgctgcag atcggaaaac ttgccgggat tccggtcaaa ttgcactgga
    10141 gtttcctcct cgtgatccct ctctttgcct ggatcatcgg gagccagatc cggctcacga
    10201 ccgagttgat cgcgaccctc tttggcgttc cgatcgacgt gacgctgatt gcggccgggc
    10261 tcaaccccta tatactcggg agcatcgtcg ctctcggcct cttcgtcggc gtcttcatcc
    10321 acgagatggc tcactcgctc atcgcaaagg caaaaggcat cgagatccac agcatcacgc
    10381 tcctcatcct cggcggcgtc tcgcagatgg aggagacgat gccggacccg aagatcgaac
    10441 tccccatggc gctcgcaggc ccgatgacga gccttgcgat cggcctcatc agcgccgccc
    10501 tggtctatgt cttcgacgcc gcggtcgccg acccggcggt cgccggggtc ctggtcttca
    10561 ccttcggcta cctgggcctc ttaaacatcc tcctcttcgg gttcaacctc ctcccggcgt
    10621 tccccatgga cggggggcgc gttctccggg catggctcgc ccggaggatg ccgctctccc
    10681 gggcgacccg gatcgccgcc gacgtcggga aggggtttgc cgtcgtcttc gggatcgtcg
    10741 ggctcctctt cctcaacccc atcctgatca tcatcgcctt cttcatctac atcggggcaa
    10801 gccaggaggc cacggtcctc cgctacaaca tactgctgca ggacatcacg gtggcggatg
    10861 cgatgagcag ccccgtcgcc accgtggagc cgacactgcc cctctcccgg ctggtggaga
    10921 tgatgtacga gacgaaacat ctcggctttc ccgtggtcga ccggggggcg ctcgtgggga
    10981 tcgtcgccct cgccgacatc cataagataa cgccgcagga ccgggaggcc atgcaggtgc
    11041 gcgacgtcat gaccagaaac ccgaccacgc tcccgccgtc ggcaccgctc ctcgatgccc
    11101 ttaagatcat aacgggcaag gatatcggga ggatcccggt cgtttccgac ggcaccctgg
    11161 tcggtatcgt cacccggacg gacgtgctcc gggtgatgga gttgcgggag gaggactaaa
    11221 acttcatcgc accctcgata cccgtctccc ccacaatctt ttgaaactcc gcgatattct
    11281 caaacggccg gcgggcggcg atggttgcgg ccctcttctt gccgacgccc ggtatccacc
    11341 ggagagccgc gaccgggagg gtgttgacct ccaccgggca ggggagggcc gtcaccgacc
    11401 gcatccccca gtccaccacc acggcgtcga gcaccgtccc cgccgggagg tcgagcggaa
    11461 tcccggcgag gatcgggtac gaccccatct gccgcccaaa cgacggcctg cccgctatct
    11521 cgacgacgac atcgcgaagg accgtcccga tggggaagac ccggcggagc atcggctcgt
    11581 cgaactctct tcgggtccac tccttaaacg cgcggaaccg ggcgtcgtgc gcaccgaggg
    11641 tgttcttctc gtaggccgcc gtcccctcaa acggcatcac ctgccggatg ttcacccgcc
    11701 ggacgaggag accggcatcg agcacccgcc ggaggaacgc ctcgttcgca tcgaacgtcg
    11761 ccggggtctc ccccgcgagg ccgacgacga agttcaggcc cggcaggagt tcggggatgc
    11821 ccttcgtccg cttccccccg acgtcgtttg tgacctcgat cgcgcgaaag acgtcgtccg
    11881 gcatggcttt gaggttgttt gccgcgacca ccgccggatc ggcggtctcc atgccgaagg
    11941 ccgcggtgtc gccgggggta tggccggcga cgatcgcccc gagcgcctcc cgcgccgcat
    12001 cctcgtgatg ggcgatggtg cccgggttga tgttgtcgac gtgcagggtg gcaaggccgg
    12061 gagcgctctc ccgcacggcc gagaagagtt cggcgagcac ctccgggcgc ggtgccggga
    12121 actccccgcc gtggctctgg taggcgagga ggtcgggttg ccggccaagc ctgaagtggc
    12181 gggcgcccgc cgcgtgaagc gccgccacct ccctcgcgat cccctcgatg ctccggtagc
    12241 gcgggagacc gtagaacggt tcggtgcaga aggagcatcc cccgaccgtc gcccgggagc
    12301 acccggtcgc ggtctcgagc tcgcacatga cgtacgggaa cgccgggtgc cgggcgacga
    12361 taccggcgcc tgccacggcc cagggatcgg tcagggagta gtcgcctgca ccggcaggtt
    12421 ctccgcccga gagccaggca tcgagcgcca ccgcgggcga tcccgagagc gtggcgtcaa
    12481 acccggcaat tgcctggcgg atggctttcc tcccgccccc gggagcgtag ccgaacgcga
    12541 tcggcccgcc tatggcggtg gccggttccc ggagcagggt cccgagctgc tggatctccg
    12601 tgagggtcgc gggtttcccc ccgatgtagg cgccgggcac ggtcagcccc gctatcataa
    12661 cgacgaggtc gctgtggccg gcgacgagcg agggatcggc acggacctgg tcgatggtga
    12721 cgtagcccgt ggtgtaaccg tgttcgacga ggacgccggc gacctcgcgg atgtaagggg
    12781 atatgtaggg cgcgacgccg aggcacgcgg gctcgtcgac gtagccgtcg atgatgaggg
    12841 cgttagaggt catacccgat caggcggagg agctctccgg cccagtagag ctttccccgc
    12901 ttgacgggat cgacgttttt atcggcaaga tcgaacccga tgatccggat cgatgccgca
    12961 ccgaggtcgt cggcggcaaa gaccgcccgg tcgccgtcgg tgaacccgcc gaagttgtgg
    13021 atgtgggcga acggcgccgc ctgggtggtg gcgacgagcg ggcccgggag gcggggaacc
    13081 cagtggcgga tgagcggcat gttatcgccg tgggcatgca cgaccatcac cgtcccctgc
    13141 cgggagaggt cgacgaagac gtcggtggcc ccatcaaggt cggtgaagac cgcgtcgggc
    13201 cggatgccgt gacctgcgag cacctcggcc gcggcatcgg cggcaagcac cgtcccctcg
    13261 atccggtcga gctcgtcggg gagggaggga gcgtttccgc agaccgtcac cgccctgccc
    13321 cgtatgagcg cctcgaggag tccgacgtcg tcacgaccgt cggcaacctc ttcgagcagg
    13381 cgggcggccg cttcgtctgc atcgcgctcg aatccaaaat aatcaagaat tgcagtgtaa
    13441 tgaggttccc agtccgcaaa cctcattgct tgccctcgtt gttatccagg ccgacgatgt
    13501 ttgcgaaccg cttcagtatc gggtcgatga tgaggttgag cagttcctcc ttctccttca
    13561 ccatcgagaa gtagctgagc ggtatcatcg cggcggccat cgtggcgtgc ccgccggcct
    13621 cgccgatagg cccgaacgcc tcggccatca cgttcccgag gtggagcctg atatccttgt
    13681 tccggccgga tatgacgatg ttctggtcgc tgatgccgta gaccagcgcc gtgttcaccc
    13741 cctcgagatg gatcaggata tcggccgcct gcgggagcgc gtcgcgattc cggatgaaac
    13801 cgacgttcga gaagaggtaa ccgctcctta tccgccggtt ccgtatggcg ttccccatga
    13861 tctccacggt ctcctgcgat atggaggggg aggtaatctt gtcgaggagg tccgagtcgg
    13921 tcagggggag gaggaacgcg gcgtagttga ggtcctgagg ggtgacgttc ctcttgaaat
    13981 cccgcgtgtc ggcccggata ccgtagagga gcgcggtggc taccgatttg ctcaccggta
    14041 tatcgagctc catgaggtac tgggtcatga tgcttgccgt ggcaccgaca ccgggccgga
    14101 tatcgacgaa gtcggcggcg ccgggggggc gctcgccgtt cttgtgatgg tcgacgatga
    14161 tgttgacccg ggtggtcttc gggagctcgt tgttcgcgcc gggcgcggac gagtcgacca
    14221 gggcgatgta gttgcactcg ctaaggatct gcggggtgag ccgctccatc ttgatctcga
    14281 ggaggttgat gaacgcccgg ttctcctggt gcccgatgtc tccctcgtag aggatgcggc
    14341 agttcagctt gttgtggctt gcgtggcggg cgatcatcga gagcgccatg gcgctcgata
    14401 tcgagtccgg gtcggggttc aaatgggtga cgatgcagag cgtcccctcc caggcggcaa
    14461 gcaggtcgta gaggcgcagg gcaagcctcg acgagtggag tttcctgatg tggtggatgg
    14521 cggttcgggc gaccacctcc tgcgggtaga ggacgatatc ggcgccctcc tgctggagga
    14581 ggtcgacgct gaccgggtcg gtggcgcgcg cgatgacgta tgtcgccggg tagcggttct
    14641 tgatggtctt gagggcggtc aggttggcgt cacgatcgtt ggtgaggatg aacgcgacct
    14701 ccggcacggg caacccctcc atgaggttcg ggtcgcggag atcgcggacg agcgcttcat
    14761 acttctggtc ccggaggtcc tccacccgct tctcgtcctt atcaacgatg atgaggtcct
    14821 cgctctcctg cccgagttcc tccgcgacgt tatagccggt gctcccgcaa ccgaagatga
    14881 tgtacttaat acgttccttt gaaacgttct ggaccgcctc aggcatgcgt acaatggtgt
    14941 aacttctcat ggtattaagg gattccatat cggcgaatta cgggaggacc ccgtaaggtt
    15001 tatacctctt cacgtactat ctttttaggt cagatacagg ggcctgtagc ttagtcggca
    15061 gagcgacgga ctcttaatcc gtaggccaag ggttcaaatc ccttcaggcc cgtcgaaccc
    15121 tcattctttc ctgacctgta ctcgatatcg ctggcgacga ccgcccggtt ctcgcgatcg
    15181 cccttccgaa gcggttcttc attgccggcg gggcgcacgg ccatcgggtc gaccgtcatc
    15241 agccttcacc ccggtcacgg tttctcacag aaggccggtt gttgcggatt cccgggggtt
    15301 ccggaggcac atatatgccg ctgctatata aatgctgctc aggcaaaaaa ttcaagagag
    15361 ttccgtgagg atggcagaga tcgcccgggg ttcctcgttt tgcacctgcc cgccgcaggg
    15421 gcgccggcag ggtcggaagg ggagggggag tatgcggccg gacttacgct cccatcaccg
    15481 cccgatcttt aagagcatct cctcgagcgt cgggtagtgc gactcgatcc ggtcgatccc
    15541 ggcaccttcc gccgcgagcg ccgcggtcgt ccggttcaac tcgtcgatgg agcgcgcctc
    15601 ggcaaggtac cggccgtcgt gggcggagta ctcgaccggc ggggcaaagg acgagaggtc
    15661 cggaatgcgg aagaagaccc ggtaggtgat cgaaccgaag gtctccctga gttcggctat
    15721 ggtcccgccg gccaccaccc gcccccggcg caggatcagg acccggtcgc agacctcctc
    15781 cacctgggag aggttgtggg ccgagaagat caccgtcttc ccccggtccc tgaggccttt
    15841 gatgtactcg agcaccgacc gggacgtcat ggggtcgagg ccggatgtgg gttcgtcgta
    15901 gatgaggaga cccgggtcgt gcatcagcga tcgcgcgatg gcgaccttcc gtttcatccc
    15961 cttcgagagt tccccgatct tcttcccatc cggctcgagc gcgagccccg cgaggaggtc
    16021 gtcgcgccgg gtccgtatgg ccgccttcgg cataccgtat atctcgccga agaacgcgag
    16081 gtaggtatcc gtggtcatcg tctcgtagag ccgtgactcc tcgggaaggt agcccaggtt
    16141 ccgtttcagg ttaaacggcc ttctcacgac atcgatgccg tcgatcagaa gattccccgc
    16201 cgtcggcgtg acgaggccgg ccatgatctt caagagcgtc gtcttgccgg ccccgttatg
    16261 cccgatcaca ccgagtatcc gtgcgtcgtc gagatcgaac gtgacgtcgt cgagggccgg
    16321 gaagtcgccg tagtgcttgg caagagagcg ggcggtgatc atcgaatctt cctatggctc
    16381 cgatgactac taataggttg ctccacagag gtatgctcct ctgagactac cggggataac
    16441 catgagtctt gcttcctcca tccggacgat cgccgtctgg gagatgaacc ggtcgatgac
    16501 cacgatggga agaggcgtcc tgccgctcgc tgcgggactg ctcgtcctcc tggtcctggt
    16561 gaccgcgttc gccgcagaga gcggcatcca tatgcaggac ggcatctacc ggatcggcat
    16621 cgacgacccc gatgtcgccc ggatagtcga cgccgacacc cgcttcaccg tcgagacggt
    16681 cagcggaccc gctctctgga acaaccggtt cgcatacgat atcgtcgtca tccgcggaga
    16741 ggtctacgcc gccgataccg gcaaagggcg ggcggccctc accgcgctca aacgcgatta
    16801 cgagggttac gtctcccgga tcgccgcagg agaacccgac ctcttcgccg gctacccgct
    16861 ctggatcgat ctggagtacg tcaagagcga gatcgacttc ctggcgaccc agagcggaca
    16921 gcaggtcggg gctccggcaa atgcccggga accccccgtc ccgtccggcc cggtggaggc
    16981 ggtgaccctc ccgccgtcga cggtcccggt ccacgaggag gacctccggc agcaccttgc
    17041 cggtatggag aaaacaaatc ccctctcccg ctacaccggc atcgtatcga gcggttcggc
    17101 aacagacgga ctccggacgc cgtcggaact ctcgcccccg ctcccgtacg atgcgctcat
    17161 cctggtcttc gtcttcatct tccccctcta cttcacctcc cagttcttca tgatgagcgt
    17221 gatgaacgag cgggtgggaa gggccgggga ggcgctcctt gccgcccccg tccggccggc
    17281 ggcgatcatc gtcgggaagg cgatcccgta cttcgcgatc atgctcctcg tctcgaccgc
    17341 gatcaccttc ttcatcggcg caccgctcac gatactcctc ccgctcatac cggtcatcct
    17401 cttcttcctc tcaagcgccc tgatcatagg gatggccgca aggagcttca aagaactctc
    17461 gttcgtctcg atcttcttct cgacgcttgc cacgtcctac ctcttcttcc cgacggtctt
    17521 tgcaaacacc cacgtcgtga gcctcatatc cccgctcacc ctggtcatcc tcgaactcca
    17581 gggcgaaggg ttcaccgccg cgggatacgt ctactcgacc gcgctcttct ttgccacgag
    17641 cgccgtcctc ttctacgccg ggacgatcaa cttccgcgaa gagcgcctct tctcggagaa
    17701 atccctcaca tcaaggctca tggacttcgt cgcgggcgcg atcagccgcg accgcccgca
    17761 cttctcgctc ttcctccttg ccgccttcac gataccgttc gtcttcatgg cccagatgat
    17821 gctgctcgtc ctcttcttca acatcccgat gccgctctcg ctcgtcctcc tgacggtctc
    17881 tgcggcgttc atcgaggagg tcgcgaagtc cgtcggactc tacgcggtag caggcgagcg
    17941 gccggggttc ctcacgccaa agaacctgct cgtgggagcg gccgccatag gcttcgggtt
    18001 ccttgccggc gagaagctcc tcctctttgc cacgctctct cagatcaccg agtcgatctt
    18061 cgggagcgtc ctcttcctct ccctccaggt gctctggatg ccgctcctcc tccatatcgc
    18121 gggcgtcctg atcaccggaa ccttcctgct cgccggcggg aagcgcgcct acgtgcccgg
    18181 cctcgtcgtg gcaagcgtgg tccacagtct ctataacctc cacttccttc tcggagggct
    18241 ggtatgaacg cccgccacat cggtatcgtc gcaaagaagg agctcctcgg cctctctgcg
    18301 gagaagacga tcgtctttgc catcctcctg caggtcttca tcgccatgtt ctcctcgttc
    18361 ctgatggtgg ggctcaccgc catgtacgac ccggaggcgc tcgagggcta ctcgacggcc
    18421 acctacggcg tcggttacgt cggggcggac tcggccctca tcgacgagtt cgagaagcgc
    18481 gacgacctca tcctgcaccg gatggacctc tccccggcgc ttgcagcgct gcgcgaccgg
    18541 aagatcgcgg cggtggtcta tgtccccgat acgccccccg acgccgaagg accggtcgcg
    18601 ataaccctct acctcgtcca gaacgacctg cagtcgagcg tcatcaacgt gaagatcaag
    18661 gaggtcctcc agggctacga gagggaactc agggaggtgc gggccgaccg gatgaccgcc
    18721 ttccccgtcg ggctgaactt ccccgacgcc ggcgatgcgg agagtttcta cgagttcgtc
    18781 tacggcctcc tcatcccgct gctcgtcctc ctcccggcga tcgtatcggc ggccctgatc
    18841 atcgacctca tcaccgagga gtaccagcgc cagaccctcg acaccctcct ctcgacgccg
    18901 gtcacgttca cggagatggt cgccggaaag attgccgcct gctttgccat cgtcccggtc
    18961 caggccgggg cctggatact ccttctcgag ctgaacggta ttgcagtagc aaacgccgtc
    19021 ccgatcctcc tccacgtctc ggcggccggg ctcttcctca tcctgctcgg tgccgcgatt
    19081 gcgctccact accgcgagcg gaccagcgcc cagttcgtct tctcgacggg gctcgtcgtg
    19141 gtcttcctcc tcgccatggc catcccctat aacccgctca acatcgtggt caggctggcg
    19201 gtggggaccg ccggcccggt tcactggctg atcctcctcc tggtcagtgg cgcgacggcg
    19261 ctcctcgcct tcgccgtcgc ggcctacggc cggcgggtcg ggaagatctc cccgacagcg
    19321 gtccggtgat gggttcggcg gggagtcttc tggtgggtcc gccctcccgc cgcattccat
    19381 caggggaaaa ttatatattt taggttatgc aattcagccc atcgatgacc cgccttcgct
    19441 acctcaccac cacctgtgca gtctgcggcg agtcctgccg tttcacgatt cccccgggcg
    19501 ccgtgagcgc cgggtcgcgg gacctcgaca cccgccctgc cgaacccctc cggtccacca
    19561 tatacgcgtg ggtgaaacga tgcccttcct gcggctactg cgcccccgac ccgggcaaag
    19621 ccctcgcggg ggcggccgat gcggtgagac tcccgcgcta ccgctggcag cttgacgccc
    19681 gcgggtttcc ggatatcgcc aacaccttcc tctgctggtc gatcatacag gaggacctcg
    19741 gcgccccggc ccaggcggcg tgggcgtccc tccatgccgc ctgggtctgc gacgacgagg
    19801 gggaggatgt gccggcccgg gtctgccgga ggcgcgccgc cgatctcctg cagcgggccc
    19861 gggagcagga cgaacgcctg gcccccctgg aaggggccga cgaggcgctc ctcgtggacc
    19921 tgctccgccg ggccggccgg ttcagggagg cccgcgccgt ggcggcgcgg gtgctggatc
    19981 aggatccgca gcctctcatc gccggtatca tgcggctcca ggtccggctg atcgctgcgt
    20041 cggatcgagg ggcccacacc gtcgccgagg cgcggcggtc caggcagccg acaccgctct
    20101 gaccggagaa tccggcagag ccccaccatt tgtatactgt tcttacaata ctgtacatca
    20161 tggatatcgc gatcttctcc cccggcatct acacctacgg cgccatgctg atcggcggcg
    20221 tcctccgtga cgcagggcac cgggtctccc tcacccgggc gcccgccgtc ccggcggact
    20281 ccctcctcct cgcaagcctt ttttcgaccc agcacctcct cgaccccgcc atccgggacc
    20341 tcgtccggac ccaccgcgag aagggaggga aggtctacgt cgggggcccg gtctcggccg
    20401 cgccggagat agttctcgga gagctcgccc ccgacgccgt ggtggtcggg gagggcgagg
    20461 agacggtggt ccgcctcgtc gcggaggggg tgtcggccgc cctccccggc atcgcctatc
    20521 ttgacggcgg ccgaccggtc gtgaaccctc ccgccccgcc ggcctcgatc gaccggccgc
    20581 tccccctcat accgggcgat atcgggagcc agagcatccg gggcgcgagc gcctacatcg
    20641 agacccaccg ggggtgtatc gggggatgca ccttctgcca ggtgccccgg ttcttcggga
    20701 ggaagatccg gagccgcgaa ctcgcgagca tcatcgagga ggtgagggcg ttctcggcag
    20761 ccggggcgac gaggctctcg gtctccgggg ggaccgggtc gctctatggg tcggccgacg
    20821 gcgagatgaa cccggaggcg ttcatcgccc tcctccacgg catggcggag gtgatgggag
    20881 cgcgaaacgt ctcctccccc gacatccgcg tggactgcat caccgacgag atcctggacg
    20941 ccgtccggcg atacacgata gggtgggtct tttttggtat cgagtcgggg agtgcccggg
    21001 tgctccggtt gatgggcaag ggagcaaccg tccggcaggt cgaggaggcg gtggggcggt
    21061 gccgggagca cggcctccgg gtcgcgggaa gtttcatcgt cggctacccg ggggaaacgg
    21121 agcgggacta cgaggcgaca aaagacctga tcgcccgcct cgccctcgac gacgtcttcg
    21181 tgagcggcgc cgagcctatc ccggggacgc cgcttgccga cctcgcggtc aggacgcccc
    21241 gggataagaa cccggcgttc gtgccgcaca ccggtgagta ccgaccgctc aacctcaccg
    21301 agagcgaagc acgatgcttc gacctgatga tgcatgccga tatgttccgc ccgcagatgc
    21361 ggctcgtgac cgacgaggtc tacgccacct accttggcga ggcaaggaag caggggcagg
    21421 atatcagggc ggcaaccgaa cttatcctcc gctactcgcc gtccggtgca taagcgccgc
    21481 gccggcagcc tcatctcccg gtttttcggg gcgggaaacc cttaagtata ctcgatcctc
    21541 tactcccccg tcctgaaggg gatatccctt cgcaggtaaa caggttggag gcgagacgat
    21601 tggcagagag agaggcagga caggtactgg caacccggcc ggagcctgga gagaggatag
    21661 acctgatgcg cgcacgcgac gtggcggaca tcaaggtaca gaacccggaa ggggagaaca
    21721 tcggcgacat caccgatatc gctatcgacc gcgatggtgg ctgcatcgcg tacgcagtgc
    21781 ttgcgtacgg cggtatactc gggtttggcg agaagcactt tgccataccc tgggaggcag
    21841 tccgggtccg ctcccgcgaa aagatcgcta tcgtggatac ggataagaag acgctcgata
    21901 acgcttccgg tttcacccgt gacaaaatgc cacgggaagg cgactggagc gtgatcagga
    21961 cacccccgcg cccgactgcg gcaaaagagc gtcctgctgc agaaggagag cgcccgccgc
    22021 cgaaagagga gaagccggca gcaccggcag agcccgcacc ccgcactgta gagagttaca
    22081 ccccggtgcc gccgcccgtc cagacggtcg cgggcggttg gggagagaag gcgatgaaac
    22141 cacgaaccga ggagcagccg gcccgggccg cgggggccgt cgtggaggag gtccggcgtg
    22201 aagagccggc acg
//
LOCUS       DAOSII010000017        22099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_2604_length_22099_cov_42.434086, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000017 DAOSII010000000
VERSION     DAOSII010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 22099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22099
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2604_length_22099_cov_42.434086"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PLV90_02470"
     CDS             <1..506
                     /locus_tag="PLV90_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HOF96009.1"
                     /translation="GAYTLVVWENKTAPILGGREQTGIPKIYADIEDLHIVRPHYATT
                     VSYEGSTFLTMNFEAAGPITGRDLDTLKSQFISMNTIGWRYIPKVGAPGAELSQFVLY
                     PQGMEVETAEIGRGSLKWTELTPMQSPVQYYIVNSLASLPMKRVTQAVLIEGRAILHA
                     MGARAIE"
     gene            521..1360
                     /locus_tag="PLV90_02475"
     CDS             521..1360
                     /locus_tag="PLV90_02475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOF96010.1"
                     /translation="MVDPDYYKSKVCIVTGANSGIGYAISEELLKRGAIVYMAGRSRE
                     KVAAAAGQLSAYGADRVRPLIMDVTKQEQVQKGIENTAAEAGRLDLLFNNAGVGGTIP
                     FEKATLDDWKTIIDTNIWSVIYGVHAAVPIMLKQGFGHVVNTSSIAGIAPPPFQALYS
                     LTKYGVTGLTECLRYEYADRGLYFSTICPANIATPIFNKSIDGQAHGELRIPDDAYPV
                     DKAASLILDRVAEHKGIIVVPEEPYTDLWKGYVLGNPEVEERLLQMARERREAFAKGG
                     AYF"
     gene            1729..3063
                     /locus_tag="PLV90_02480"
     CDS             1729..3063
                     /locus_tag="PLV90_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96011.1"
                     /translation="MTGQNVLIFVIVALIAVVAGIMIAFAPGETVPAEKASPLDAMSA
                     LATEVAGQIDGDAMAALKPGDESTPAFAAIHDRLNAFRSQHPDIIYIYTLQRTGNAVE
                     YVVDADYGNAGARAIGGGHQVVDPKTPLFAGFEKPSAEVYSAIYGYAPVKNGGMVCLE
                     PEGPIAQERLEALAVEVAGQIDGDAMAALKPGDEETPAYVAIYDRLNAFRAEHPEIAY
                     AYTMRKAGNTVEYVVDADYGSDDGVAIGDGYTPTAIDGVLLRGFEKPSAEIYSLICGY
                     APVKSTSGAGVGMVAVEVGNPITEERLKALAVEVAGDIDGTAMAALRPGDENTPAFTA
                     IRDDLNAFRDATPGVVYVYTMRKTGNATEYVVDADYGNGDAPAIGYTYVPTEEDAAFL
                     AGFEGPSAEPGFYVDDWGNETATVLSGYAPIRDAVGAIVGLVGVDMGIVHES"
     gene            3191..3493
                     /locus_tag="PLV90_02485"
     CDS             3191..3493
                     /locus_tag="PLV90_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96012.1"
                     /translation="MDRTTSRPARCATGSPKGDRLALLEEIAAFVREHADILARYHIH
                     TMADLNRIEQECWRLHDEACSRGACGTAGELAELEYLIGRAKEMKAKRMEGERGPG"
     gene            3746..5413
                     /locus_tag="PLV90_02490"
     CDS             3746..5413
                     /locus_tag="PLV90_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HOF96013.1"
                     /translation="MTSGSPIDDKSPWLQVIAGFLVLTIIVLIPIGLSWYLHPAPVHV
                     RILAVNDFHGQLTAGKSLSGEPVGSAPVLASYLNAAAGRPATFIALPGDIVGASPPES
                     GLLLDEPTILFWNGLAGDCPQIATFGNHEFDRGTDELMRMVDGGNGATTIAHLVDPYP
                     GAVMEYVSSNVVWRVNDTPIAAPYTIRDAGGVGIAFIGATTIETPSIQKPANIEDIVF
                     KDEAESINRYIPEIQEKGVHAIIVLLHEGGSQDAYDGPTREGGNVTGRITGIVAGLDP
                     DVDLVLSAHTHEFTNACLENAGGRSVLVTQAYSYGKAFADIELVIDPATGDVTHRTAE
                     IVPAYANRLSGTDPGAAALLERARDAVRPLTERVVTSASASITGQATGAGESPLGNLV
                     ADGQRAAMQADVAFITTGSLRADIPQGNVTWGDLYAVQPFASTVLSMTLTGEQIRDVL
                     EQQWQTPLPPHNLAVSGLSYTFDDGKPAGEKITEIRVGGAPLEPGANYTAAMVDFLAT
                     GGDGYTVFTKGVNPAYGPLDIDALVTYMGALPEPIGVKAEARIAKAA"
     gene            5539..7695
                     /locus_tag="PLV90_02495"
     CDS             5539..7695
                     /locus_tag="PLV90_02495"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOF96014.1"
                     /translation="MTSRLLFYRTRIIPALALFCIVTLAISSAGCTTTAADPIRIGVL
                     LPADGPVALHSLEGLAWAADAMNRQGGRMIELVCRDTSTGNISAYAEELAGRDDIAIV
                     IGPAMSAELIDIAPLVTGRGKLLISPSVTMELATEELNDNSLLWRTSLADGRQVETIF
                     LRLRGDGVRNVSLVTGATPYGETFARLAPEAAAKNGIRITGAASLSASDDLSEIARQI
                     VKEEPDIVVAAVYPEEAIGLVNVLSPDRLFFSDACRSSYLIEHLGERAEGIRGTSLAP
                     DPSTGYAIAYEEIFGEAPPAFAAQTYDAFLLALYTAARQEAAPGESLAASIRWVVTGD
                     GTVKGWDPEEAGEAMALLRAGAHPAVTGASGPLRFGEHSSRGPLAGYYSIWAIEGGAF
                     SEGSPVPSAAIDPYIPDLSFAGSAGDRPVVWMIYPLEKGDRSFADSAYRGLFRAQESF
                     SFTKREFTYADLALLDAVFSLQNFTEKPDLVITEGFEFTGAARAWAAANPDIRFVTLE
                     QEDYGLPNACDVVMVPYGASYLAGVLAANITRTGHIGAIAGAPVAVIEPFVRGFTGGA
                     AAYDPSVNVTVLYVGESFEGFGMPERAGDLARDLRADGVDVILMAAGASGTGVVDAAR
                     ETGDIFLIGVDTDQSYVAPNLVVASVVKGIDTIVYHAVANEIAGRFAPGQEVWTLENG
                     GTGLVLSSRFAEHTWVDTGWRDRAIAAEKEYLKAVA"
     gene            7836..8213
                     /locus_tag="PLV90_02500"
     CDS             7836..8213
                     /locus_tag="PLV90_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96015.1"
                     /translation="MAWQDLAITGITILFAVMLLPQLRDVVSRGVVLNCFSAFFTSVL
                     SYTLAMVFATLGLWISVFGQALVASVWLLLAYFSLRNVQASMYPDEALTSVARDFFTV
                     WLQGVAFVVSGGVTGFFSRLRRG"
     gene            8256..8555
                     /locus_tag="PLV90_02505"
     CDS             8256..8555
                     /locus_tag="PLV90_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96016.1"
                     /translation="MEHTASGADRSPGAVRKGQIDLIAEIAAFADEYGDILARYHRYT
                     MDDLCRIEGECRRLQDEARRRETWGIADELAGLEYLIDRAKAMRAARMAEEDSRG"
     gene            complement(8623..9726)
                     /locus_tag="PLV90_02510"
     CDS             complement(8623..9726)
                     /locus_tag="PLV90_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96017.1"
                     /translation="MFSYLSKRQASTAIILTLLVFMLHYGFSLLFPGYLYLEEASDLI
                     AIAAQSGAIGFQLAGARYLVARLKRTFKDEFPAVPGRGGGDCPYMAARSVLCNRAHYI
                     AFIAAFLSPFIVITLVSLQPDSAPRHLLSLASRSIPGLTFDVFNYGTAYANYWLLGTI
                     AWILLGVVVALNGMEGAGCSVYDAVDIYSPDRIGGLSPVRRYLMSALSIFLISIALLV
                     MGYINLLESFIGSSQGLFHFFFDVVAEICVFSVLSLSGVALTVVGLNRLSAICLKKIE
                     DRVNAVNERCRTFQENLFSFSPDELERRGDEVGSLKLVVEALSDERERLLQRYSDCSG
                     FNARMALRIFVAYIPPLVTIAFQVSQLLPRLRV"
     gene            complement(9726..11327)
                     /locus_tag="PLV90_02515"
     CDS             complement(9726..11327)
                     /locus_tag="PLV90_02515"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOF96018.1"
                     /translation="MKIGMTRICHIAGFFLLVIAISATVQSVQPADAVQADTSNPALQ
                     AVPGADNFPIVQITNSTRDQVLPRISGNRILWMETDRINTRNLYLYDIANRSAEMIIE
                     HFSPRTSPEISGNWIGWVEDQSDSSGKIFPCIRVYDIRSGTITQITQYPAMPYQTSES
                     QDITWERESTLAISGDIIVWHDKRDGNMDIYYRNLSSGVENPIARSAADETCPSISGD
                     RIIWAEQHGTKGYTIHLYNLTSNEHDRITDLPVMRRNQVISGDYIVWSEWREGSYDIC
                     CYDIASGNTTWITDEPGHDLWPAVSGDAIVWSVYGRGSDDNGISAYDLSTETGTEILA
                     DLPHPVWPDIDGGKVVWSDNRTGNYDVYLCSLENRSDAAPGLYTVHLNSIPSGADISV
                     NNEPYGRTPATLSFDRTGICPIGLKKNGFIPYTTTLDISASLTFVVNLERELVGPVSG
                     PRPILMSITVDSVPRGANVSVDGEVYGTTLFGMDRLPSGDHEVELTLEGYQSNRTTVN
                     SSEPINITLIPGTDADRPLLMERYA"
     gene            11759..12760
                     /locus_tag="PLV90_02520"
     CDS             11759..12760
                     /locus_tag="PLV90_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96019.1"
                     /translation="MNVPDVRYTVRNPTAEPVWVRLVSEYPGYSAPTVSVSEIGAGSP
                     ATFDHFVVLDREEIQEIRAPARVAVHYRIEYWDGGNWTVHDEQTDPVTFYPMDQMVWA
                     TEDKDGVITIYHGLIAIFVTPQSPGVAALAAKAKERATGEFDRRYVDYGMERTLPGYA
                     LPNQRTTYADTKNRTALQVKAIYNALKYDYNLSYVDALVAFGMGDSQRVSTPDESLAT
                     GSANCIDGAVLFASAIERLGMQPYIVVVPGHAYVAWKTDKAGTEVDALETTWVSSCDF
                     ERAYDAGTKRYKEDAKSGRMELYQSLFDRRLSRNEYDSIYVLVEIRWLRSQGILPMK"
     gene            13077..14975
                     /gene="acs"
                     /locus_tag="PLV90_02525"
     CDS             13077..14975
                     /gene="acs"
                     /locus_tag="PLV90_02525"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOF96020.1"
                     /translation="MAENFNVRLNEAKYYTPEASCREQSWIGDYNRAYQAFLADPDGF
                     WDSAARELEWFQPWDQVREWEYPHARWFVNAKLNITHNCLDRHAHSQRRNKVALMWRG
                     ETEDEERILTYRQLLQEVCRFANGLSRLGVRKGDRVCIYMPVVPEQIVAMLACARIGA
                     IHTVVFGGFGVSALNQRIRGTAAKVVITADFTYRRGKAIPLKNIVEEAVVKAPSVERI
                     VILRRDAEKPVEIHPEMEVDYHDLMNGVGRECPPEPMDAEDPLFILYTSGTTGSPKGI
                     VHACGGYMVGTYYTTKHIFDVKDSDIYWCTADPGWITGHSYGVYGPLLNGATCLITET
                     TPDYPTPATNWDLIEEYGVTIFYTAPTAIRMFMRVGEEWPNRYNLSSLRILGSVGEPL
                     NPEAFEWFYQTIGKGRCPIVDTWWQTETGMQMLTTTIGEPMRPGFVGKPIPGVVADVV
                     DKTGKPVPPGTGGLLVIKEPWPSMMRTIWNDDERYCKYWDTIPGCYTAADLAVKDEDG
                     YIMVIGRSDDLIVVAGHNIGTAEVESALVSHEAVAEAAVIGKPDALKGNTIKAFVILR
                     DGRQPGDKLKNDLTYHVRLTLGPIAIPSEIEFVRSLPKTRSGKIMRRVLKAQELGMDP
                     GDISTLEE"
     gene            15018..15581
                     /locus_tag="PLV90_02530"
     CDS             15018..15581
                     /locus_tag="PLV90_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HOF96021.1"
                     /translation="MNERRPEESLKIENIVASAKVTDSLDLPSLASQLKDAEYNKKRF
                     PGVVLRMQDPKIAALVFGSGKVVLTGAKSIDSLSRGLDILADQLRALGIDIPEDLTYK
                     IQNIVTSADLGTPINLNKIAVGFNLDKIEYEPEQFPGLVYRLDDPKVVVLLFGSGKLI
                     ITGGKVPEDAKRAVQRILSELSGLGLI"
     gene            15931..16311
                     /locus_tag="PLV90_02535"
     CDS             15931..16311
                     /locus_tag="PLV90_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HOF96022.1"
                     /translation="MRSDKVRYFTPRDEELAELLMGIGIKRNVSKVLVYLANIDEATS
                     RDIERGTDLRQPEVSIAMRYLKECDWIDTRESKSESKGRPVKIYALSRPITEIMDTIE
                     KEKKKEARHQLDLIQKMRECISMQ"
     gene            complement(16300..16986)
                     /gene="rpiA"
                     /locus_tag="PLV90_02540"
     CDS             complement(16300..16986)
                     /gene="rpiA"
                     /locus_tag="PLV90_02540"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HOF96023.1"
                     /translation="MTAEPKRNAGYRAAAMVEDGMAVGLGTGSTVFFAMERLGERIAG
                     EGLSFTGIPTSYQAAIRARRYGIPLAGLDEYPELDVAIDGADQVDPALNLIKGRGAAH
                     LREKCVCDAAREVIIVVDPSKTVDTLSAPVPVEVLPFALAGVSRRLSLLGAAPVLREG
                     VKKDGPVVTDNGNFILDCDFGVIGEPERLETAIAAIPGVLECGLFTAYTEKITVIVGE
                     KIGCRVVSLH"
     gene            complement(16983..17780)
                     /gene="surE"
                     /locus_tag="PLV90_02545"
     CDS             complement(16983..17780)
                     /gene="surE"
                     /locus_tag="PLV90_02545"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOF96024.1"
                     /translation="MKPKIMLTNDDGITSAGLWAAYEALAPIADVTVVAPATQQSAVG
                     RSISIFEPIRATKVLMNGVPAYSVGGKPTDAVIIGLFALHLNPDVVVSGINIGENLSY
                     ESIMTSGTVGAALEASNQGVPSLAFSLQVEDQGDKFDDPSQIRDRFFDAKRVVREVCE
                     RVLANGFPGNAHVINVNIPTPVRGGYEITRLAEKLFYTGVEERFDPRGRPYYWIDGPL
                     HEDAEEGTDVHAVQKGNVSITPITLDCTAFGARDELRGIFDGMVSRS"
     gene            17863..18750
                     /locus_tag="PLV90_02550"
     CDS             17863..18750
                     /locus_tag="PLV90_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOF96025.1"
                     /translation="MKVFLAEYSICNDPGLAPEGAAMLAVLSGSFERCGYDVVTPEGP
                     DLEDGIRRLAPGCDAGLVITPDRLLFRYTHLLEQFTHNIGCGSMNAAISANKQRAGAI
                     LARHGIAVPPAAGSGRRVVKPVMGCGAQDVRLTDGEPGAGEFAEAYIEGEALSVSLVG
                     SRVTGNACEYYSGNPPLLLAVNRQDIAVADDGSFQYLGGETPIHPDREEEIVDLAVRA
                     MNVLGCQGYAGIDVVVGDEIYVVDVNPRPTTSLVGIAAVMEEEIADILIRASHGEAPA
                     DVHLSGRVRFDKDGRIARV"
     gene            18747..19673
                     /locus_tag="PLV90_02555"
     CDS             18747..19673
                     /locus_tag="PLV90_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HOF96026.1"
                     /translation="MIGIDIGGANLKIVDDAGVHIHYCPLWQGAPLAGLLEPYAGSAA
                     VVMSGELADCFSTKIEGIRWIVGTVREAIPDAAFYGTDAAFHTRPVPGLAAANWLASA
                     DYLREEYADAVLLDVGSTTADVIPLTRFEDLKGLTDTRRLQKQYLVYTGMLRTNVATL
                     LSSVTLDGTVTPVSTEYFAASADAHLVLGHIAPADYTCPAPDNGEKTVDAALRRLARV
                     VCADIDEIGKSGAHQVARQFWEVQRRVIGRAVGRALFQGDAERVITAGIGADLFAREL
                     GCATLAQEIGEVSDALPAYAVREVALRRAACD"
     gene            complement(19751..20323)
                     /locus_tag="PLV90_02560"
     CDS             complement(19751..20323)
                     /locus_tag="PLV90_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96027.1"
                     /translation="MLADLVGNALTAMDGVRFTHLDACPSCGGQVTGHDLRPKRFAVI
                     LEEGEERTIRVFVKRFYCRQCGSLCYADAPFYPDTRLASPIVDLCLLLSQRMPFNQVA
                     EHLRALGIVVDRGTIRNYASRDFDAIPATELFGLLLPLSLLNLAISTLGREGCPVIGA
                     EALAACGLPPADRAAPRGRGLSEERYQRYN"
     gene            20641..>22099
                     /locus_tag="PLV90_02565"
     CDS             20641..>22099
                     /locus_tag="PLV90_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOF96028.1"
                     /translation="MTLQYVATTCPYCGTGCSFNLVVQDGKVVGTAPYRRSPVNEGKV
                     CPKGTYAHEFVNSPDRLTKPLIKKDGKFVEATWDEAYDLIAQKFKSYKPDEFAALSSA
                     RVSNEENYLMMKFARGVMKSRHVDHCARLCHASTVAGLAASFGSGAMTNSIGDIAESK
                     CVFVLGSNTLEQHPLIGRKIVQAKKNGAKVIVADPRYTPTARLADLYMPFVSGSDVAL
                     LNGLMQEIIRNGWEDKEFIEKRTKDYEKLKEVVMKETYSLENVSKISGIPVEQLKQAA
                     EWFGTTKPGAILYSMGITQHTVGVDNVRSVANIQMLTGNLGKPGSGVNALRGQNNVQG
                     ACDMGALPVVFTGYQKVIDPAAYKKFADAWGFPGGICEPKNGYEVTVMMDVLTDNPGE
                     LKCMYIMGENPMLSDPDLNHVRHALESVEFMVVQDIFLTETAELADVVLPAACYAERG
                     GTQTSTERRVQMWRKAQDPPGEAKEDWQIISELAAR"
BASE COUNT         4282 a         6951 c         6907 g         3959 t
ORIGIN      
        1 acggtgccta cacgctggtt gtctgggaga acaagaccgc ccccatcctc ggggggcggg
       61 agcagaccgg catcccgaag atatatgcag atatcgaaga cctgcatatc gtacggcctc
      121 actatgcaac gaccgtcagc tacgagggga gcaccttcct caccatgaac ttcgaggcgg
      181 caggcccgat aaccgggcgg gacctggata ccctgaagtc gcagttcatc tccatgaaca
      241 ctatcggatg gcgctacata ccgaaggtcg gggctccggg tgcggaactc tcccagttcg
      301 tcctctaccc ccagggcatg gaggtggaga cggcagagat cggaagaggc agcctgaaat
      361 ggaccgagct gaccccgatg cagagcccgg tccagtatta catcgtcaac agcctcgcat
      421 ccctgcccat gaagcgggtg acgcaggcgg tactgatcga ggggagagcc atccttcacg
      481 cgatgggtgc aagggccatc gaatgaacga ggcgaggatt atggtcgatc cggattacta
      541 taaaagcaag gtttgcatcg tcaccggcgc gaactccggg atagggtatg caatcagcga
      601 agagctcctg aagcgggggg caatcgtcta catggccggc cgcagccggg agaaggtcgc
      661 cgcggccgcc ggccagctct ccgcatacgg ggctgatcgg gtgcgcccgc tcatcatgga
      721 cgtgacgaag caggagcagg tacagaaggg catcgagaat acggcggcag aggccggcag
      781 gctggacctg ctcttcaaca acgcgggcgt cgggggaacc atcccgttcg agaaggccac
      841 cctcgacgac tggaagacga tcatcgatac caacatctgg agcgtcatct acggcgtcca
      901 tgccgccgtg ccgatcatgc tcaaacaggg gttcgggcac gtcgtgaaca ccagttcgat
      961 agcgggcatc gctccgccgc cgttccaggc gctctactcc ctgacgaagt acggcgtcac
     1021 cggcctcacc gagtgcctga ggtacgagta cgcggataga gggctctatt tctccacgat
     1081 ctgtccggcg aacatcgcga caccgatctt caacaagagt atcgacgggc aggcccatgg
     1141 cgagctgagg atccccgacg atgcataccc cgtcgacaaa gcggcgtcgc tcatactgga
     1201 tcgggtcgca gagcataagg ggatcatcgt cgtgcccgag gaaccctaca ccgacctctg
     1261 gaaagggtat gtcctcggca atccggaggt tgaggagcgg ctattgcaga tggcacgcga
     1321 gcggcgggaa gcgtttgcga aaggcggggc atacttctga taggtcgggg atcgccgtgc
     1381 gatggtgccg ctccgtcctg gccggcagcg atccggtgtc gctgttgcgt caccggacgc
     1441 ccggcaacga cggaaggaca ggctgaaccg ctcccgggct accgtggatc ttcgacgcgg
     1501 tctaccggag cgcgccgcca tggaatatcg gccggtcccg gagggcgttt gcctgccccg
     1561 gcggggcgct cgccgctatc actcccggct tccccctgtt ttccgttctt ctcccggata
     1621 ctcgacaatc cgacatctaa atctctatac ggcgatgcac agccgcgata catttgcgag
     1681 cgtttaagta tgtactcctg caataaatcc tgaaagagtg tatcccatat gacaggccag
     1741 aacgttctga tatttgttat cgttgcgctg atcgccgttg ttgcggggat catgatagcg
     1801 ttcgctcccg gcgagaccgt gccggcagag aaggcgtccc cgctggacgc gatgagtgcc
     1861 ctcgcgacgg aggttgcggg gcagatagac ggcgatgcga tggcggcgct caagcccggc
     1921 gacgagagta caccggcatt cgccgccatc cacgaccggc tcaacgcctt ccggtctcag
     1981 catcctgata tcatctatat ctacacgctg cagaggaccg gaaacgccgt cgagtacgtc
     2041 gtcgacgcag actacggcaa cgccggtgca cgcgctatcg gcggaggaca ccaggtcgtg
     2101 gacccaaaga ctccactctt tgccgggttt gagaaaccct ccgcagaagt gtactccgcc
     2161 atttacgggt atgctccggt gaagaacggt ggaatggtct gcctcgaacc ggagggcccg
     2221 atcgcgcagg agaggctcga ggcccttgca gtggaggttg cggggcagat cgacggcgac
     2281 gccatggcag ccctcaagcc cggcgatgag gagacgccgg cgtacgttgc catctacgat
     2341 cggctcaatg cgttccgggc cgaacacccc gagatcgctt acgcctacac gatgcgtaaa
     2401 gccgggaaca ccgtcgagta cgtcgtggat gcggattacg gcagcgacga tggagtcgcg
     2461 ataggcgacg ggtatacccc gacggcaatc gatggtgtcc tccttcgtgg ctttgagaaa
     2521 ccttcggccg agatctacag cctcatctgc gggtatgctc ccgtgaagag cacgagcggc
     2581 gccggcgtcg gtatggtggc cgtcgaagtc gggaacccga tcacggagga gaggctgaag
     2641 gcccttgcgg tggaggttgc aggcgatatc gacggcaccg cgatggcggc gctcaggccc
     2701 ggcgacgaga atacaccggc gttcaccgcc atccgcgacg acctcaacgc cttccgcgac
     2761 gcaacccccg gggtcgtcta cgtctacacc atgcgtaaga ccgggaacgc caccgagtac
     2821 gtcgtcgatg cggactacgg caacggcgac gcgcccgcga taggctatac ctacgtgccc
     2881 acggaggagg acgccgcgtt cctcgccggc tttgagggac cttccgcaga acccgggttc
     2941 tatgtcgacg actggggcaa cgagaccgcc accgtccttt ccgggtatgc cccgatcagg
     3001 gatgcggtcg gcgccatcgt cgggctggtc ggcgtcgata tgggtatcgt ccacgaatcg
     3061 tagtagatgg agattaccgg gggcgggatg tgcgaatccc ccgtctatct caattgcttt
     3121 tttcccgtgg tcccgggatt gctccccgcc gcttcggaaa gactaaccct cccggcagcg
     3181 atgacgtaac atggaccgca cgacttcccg gccggcccgg tgcgccacag ggtcgccgaa
     3241 gggggaccgg ctcgccctcc ttgaagagat tgccgcgttc gtccgcgaac acgcggatat
     3301 actcgcccgg taccacatcc atacgatggc cgacctcaac cggatcgagc aggagtgctg
     3361 gaggctgcac gacgaggcgt gcagcagggg cgcctgcggg actgccggcg aacttgcgga
     3421 gctcgagtac ctcatcggcc gggcaaagga gatgaaggcg aagaggatgg agggggagag
     3481 ggggccgggt tgacccggaa accgccgggg aacgacggat gaagatatcc gtgatgtcga
     3541 ggaggcaatt gcggatatcg ggaccgggag agtttacacg acggacgagg cgaagcagga
     3601 accggggctc gattgagtac cctgaacaac ctgctccgcc gcctcgctat cgatcgcggt
     3661 atccggcccc gatcgtgggc cgcgccgcag tctcgtgcgc atccattcca gctgggttta
     3721 tcccgatcag gccagaactc tcctgatgac atccggttcg cccatcgacg acaaatcgcc
     3781 atggttacag gtgatcgccg gctttctcgt gctcacgatc atcgttctca tccccatcgg
     3841 gctctcctgg tatctgcacc cggcgcccgt gcacgtcagg atactcgcgg tcaacgactt
     3901 ccacgggcag ctgaccgccg gaaagagcct cagcggcgaa ccggtcggca gcgcgccggt
     3961 gctcgcatcg tacctcaacg cggcagccgg ccggccggcc acgttcatag cgttgccggg
     4021 cgacatcgtc ggcgcatcgc cgccggagtc cggcctgctg ctggacgagc ccaccattct
     4081 cttctggaac ggccttgcag gcgattgccc gcagattgca acgttcggca accacgagtt
     4141 cgaccggggc acggacgaac tgatgcggat ggtcgacggc ggaaacggcg cgacgaccat
     4201 cgcccacctg gtcgacccct accccggcgc ggtgatggag tacgtctcct caaacgtcgt
     4261 ctggagagtg aatgacaccc ccatcgccgc cccctacacc atccgcgatg ccggcggcgt
     4321 agggattgcg ttcatcggcg ccacaacgat cgagacgcct tccatacaga aaccggcgaa
     4381 catcgaggat atcgtcttca aagacgaggc cgaatcgatc aaccggtata tccccgagat
     4441 ccaggagaag ggcgtgcacg cgatcatcgt cctcctccac gaggggggtt cacaggatgc
     4501 ctatgacggt ccgacgcgag aaggcggcaa cgtgacgggc aggatcaccg ggatcgtcgc
     4561 cggcctcgac ccggacgtgg atcttgtcct ctcggcccac acccacgagt tcacgaacgc
     4621 ctgcctggag aatgccggcg gtagatcggt gctggtgacc caggcataca gttacggcaa
     4681 agcgtttgcc gacatcgagc tcgtcatcga tcccgctacc ggcgacgtca cccacaggac
     4741 ggccgagatc gtcccggcat acgcgaaccg attgtccggc accgatcccg gggcagccgc
     4801 actcctcgag agggcgagag atgcagtcag gcctctgacg gagcgggtcg tcacgagcgc
     4861 atcggcgagc atcacgggcc aggcgaccgg tgccggcgaa tcccccctcg gcaacctcgt
     4921 cgccgacggc cagcgggcgg cgatgcaggc cgacgtcgcc ttcatcacca ccggttcgct
     4981 ccgggcggac ataccgcagg gcaacgtcac ctggggcgac ctctacgcgg tgcagccgtt
     5041 cgcctcgacc gtcctctcga tgaccctcac gggggagcag atccgggatg tgctcgaaca
     5101 gcagtggcag accccgttgc cgcctcataa cctcgccgtc tcggggctct cctacacgtt
     5161 cgatgacggg aagcccgcgg gcgaaaagat caccgagatc cgggtgggcg gcgcccccct
     5221 ggagccgggc gcgaactata cggcagcgat ggtcgatttc ctcgcaaccg gcggcgacgg
     5281 gtataccgtc ttcacgaaag gagtgaaccc ggcctatggc ccgctcgata tcgatgcgct
     5341 ggtcacctac atgggcgcac tccccgaacc gatcggcgtg aaggcggaag cgcgaatagc
     5401 aaaggctgcg tgaaggctct ccgacgggaa atccccggaa aactcttttt ccttccggat
     5461 aacccgatga aaaggcatat tcgagccgtc gccgaagtac aacgatcagc cgccggccgg
     5521 cggcgtccgg gcgttatcat gacctcacga ctgctttttt ataggaccag gattattccg
     5581 gctctcgctc tcttctgtat cgtcacgctg gcgatctctt ctgcggggtg caccaccacc
     5641 gcggctgacc ccatccgtat cggcgtcctg ctgcccgcag atgggcctgt tgccctgcat
     5701 tctctggaag ggctcgcctg ggcagcggat gcgatgaacc ggcagggcgg gcgcatgata
     5761 gaactcgtct gccgggatac gagcaccggc aacatatctg catacgcaga ggaactcgcc
     5821 gggcgcgacg acatcgctat cgtcatcggt cccgcgatga gcgcggagct catcgatatc
     5881 gctcccctcg tcacgggtcg gggcaaactc ctcatatccc cgagcgtcac gatggagctc
     5941 gccacggaag agctcaacga taacagtctt ctctggagga cctcccttgc cgacggccgg
     6001 caggtggaga ccatcttctt gcgcctccgc ggggatggtg tcaggaacgt ctcgctcgtc
     6061 acgggagcta cgccctacgg ggagacgttt gcacggcttg ccccggaggc ggccgcgaag
     6121 aacggcatcc ggataaccgg agcggcatcg ctcagcgcat ccgacgacct ctcggagatc
     6181 gcaaggcaga tcgtgaagga ggagcccgac atcgtcgttg ccgccgtcta ccccgaagag
     6241 gcgatcgggc ttgtaaacgt tctttctccc gaccgcctct tcttctctga cgcctgccgc
     6301 tcgtcttacc ttatcgagca cctcggcgag cgggcggagg gtatccgggg cacgtcgctt
     6361 gcccccgacc cttcgaccgg ctacgcgatc gcctacgagg agatcttcgg cgaagcaccg
     6421 ccggcctttg ccgcacagac ctacgacgcc ttcctcctcg ccctctacac cgcggcccgg
     6481 caggaggcgg ccccgggcga gtcgctcgcg gcatcgatcc gctgggtcgt caccggcgac
     6541 ggcaccgtca agggctggga tccggaggag gccggcgagg ccatggcgct gctccgggca
     6601 ggcgctcatc cggcggtcac cggcgcatcg ggacccctcc ggttcggcga gcactcttcc
     6661 agagggccgc tcgccggcta ctactctatc tgggcgatcg agggcggggc gttctcggag
     6721 gggagcccgg tcccgtccgc ggcgatcgac ccgtacattc ccgacctctc ctttgccggc
     6781 agcgccggcg accgcccggt cgtctggatg atctacccgc ttgaaaaggg cgaccggtcg
     6841 tttgccgact cggcgtaccg cggcctcttc cgcgcccagg aatcgttctc gttcacgaag
     6901 agagagttca cctacgccga ccttgcgctc ctcgacgccg tcttctctct acaaaacttc
     6961 accgagaagc ccgacctcgt catcaccgag gggtttgagt tcaccggagc cgcccgggcg
     7021 tgggccgcgg cgaaccccga tatccggttc gtcacccttg agcaggagga ctacggcctc
     7081 ccgaacgcct gcgacgtcgt gatggtcccc tacggggcat cctacctcgc gggcgtgctg
     7141 gcggcgaata tcacccggac cgggcatatc ggcgcgatcg ccggggcgcc ggtcgccgtc
     7201 atcgagccct tcgtcagggg gttcaccggg ggggcggcgg catacgatcc gtccgtgaac
     7261 gtcacggtcc tctacgtcgg cgagagtttc gaggggttcg gcatgccgga acgcgcgggt
     7321 gatctcgccc gggacctccg cgccgatggc gtcgacgtca tcctgatggc tgccggcgct
     7381 tcgggcaccg gggtcgtgga tgccgcccgg gagaccgggg atatctttct catcggcgtg
     7441 gataccgacc agtcttacgt cgccccgaac ctcgtcgtcg cgtcggtggt gaaggggatc
     7501 gacacgatcg tctaccacgc ggtggcaaac gagatcgcgg gccggttcgc accggggcag
     7561 gaggtctgga cgctcgagaa cggcggcacc gggcttgtcc tatcctcgcg gtttgcggag
     7621 cacacgtggg tcgataccgg ctggcgggac cgggcgatcg ccgcggagaa ggagtacctg
     7681 aaggcggtgg cgtgagcccc ggcctacggc actccgctca tatttgcggt cgccgttctg
     7741 gcccgaccgg acccggtggg cgaaagttcg atccggatct gccgatctga tatgtccgga
     7801 cgtcgatatc tgcaggcagc acggatgtga tcgatatggc ctggcaggac cttgcgataa
     7861 ccggaatcac gatactcttt gcagtcatgc tcctgccgca gctccgcgac gtggtgtcgc
     7921 gcggcgtcgt gctgaactgc ttctcggcct tcttcacgag cgtgctcagc tataccctgg
     7981 ccatggtatt cgccaccctc gggctctgga tatcggtctt cggccaggcg ctcgtcgcgt
     8041 ccgtatggct gctgctcgcg tacttctcgc tccggaacgt gcaggccagc atgtacccgg
     8101 atgaggcgct tacatccgtc gcccgggact tctttacggt atggctgcag ggcgtggcct
     8161 tcgtcgtctc gggcggcgtt acggggtttt tctcccggct ccgccggggc tgaccgctct
     8221 ggaaggtata acccttcccg cggcgagatg tgagtatgga gcataccgct tccggggcgg
     8281 ataggagccc cggtgccgta agaaagggcc agattgacct tatcgcggag atagccgcgt
     8341 tcgccgacga gtacggggac atacttgccc ggtatcaccg ctacacgatg gacgaccttt
     8401 gccgcatcga gggggagtgc cgccggctgc aggacgaggc ccgccgcagg gagacctggg
     8461 gtatcgccga cgaacttgcc ggtcttgagt acctcatcga tcgggcgaag gcaatgcggg
     8521 cagcgcggat ggcagaggag gactcccggg ggtgacacac accctccggc acgggcatca
     8581 ggatcacgct gccggcccgc tcaggcctct ccgtcacggt tgttagaccc gtaatcgagg
     8641 cagcaactgc gacacctgga acgctatcgt gacgagcggc gggatatacg ctacaaagat
     8701 cctgagcgcc atcctggcat tgaacccgct gcagtcggaa taccgctgca gcaaccgttc
     8761 ccgctcatcg ctgagggcct cgacgaccag tttcaggctg ccgacctcgt ctccccgtct
     8821 ctccagttcg tcgggcgaga aggagaagag gttctcctgg aacgtgcggc acctctcgtt
     8881 tacggcgtta acccggtctt cgatcttttt caggcagatg gctgagagcc ggttcagccc
     8941 gacgacggtg agagcgacac cagagagtga gagaaccgag aatacacaga tctctgcgac
     9001 cacatcgaag aagaagtgga ataatccctg gctggagccg atgaaactct ccagtaagtt
     9061 gatgtatccc atcaccagca gggcgatgct tatcaggaag atggagagag cgctcatcag
     9121 gtacctcctg accggagaga ggcctccgat ccggtcgggg ctgtagatat cgacggcatc
     9181 gtatacgctg catccggcac cttccatacc gttcagcgca accacaacgc caaggaggat
     9241 ccatgcgatc gtgccgagca gccagtagtt ggcatacgcc gtcccgtagt tgaacacgtc
     9301 gaaggtgagg ccggggatcg atctgctcgc aagagagagg aggtggcggg gtgcgctgtc
     9361 cggctggaga ctgacgagcg tgatgacgat gaacggcgag aggaaagcgg caatgaacgc
     9421 gatgtaatgt gccctgttgc agagaacgct tcttgccgcc atatacggac aatcgccgcc
     9481 cccacgcccg ggaactgccg gaaactcatc cttaaacgtc ctcttcagcc gggcgacgag
     9541 ataccgggcg ccggcgagct ggaacccgat cgccccgctc tgtgcagcga ttgcgatgag
     9601 gtcggatgct tcctccaggt agagataccc tggaaacagt aaactgaacc cataatggag
     9661 catgaaaacg aggagggtga ggatgatggc ggtggatgcc tgccttttcg agaggtacga
     9721 gaacatcagg cgtatctttc catcaacaga ggccggtcgg cgtccgttcc gggaatcagg
     9781 gtgatgttga tcggttcgga tgagttcacg gtcgtgcggt tggactgata gccctcaagg
     9841 gtcaactcga cctcgtggtc ccccgagggg agcctgtcca tcccaaagag cgtggtcccg
     9901 taaacctctc cgtccaccga gacatttgcg ccccgcggga ctgagtctac ggttatgctc
     9961 atcagtatcg ggcgaggtcc gcttaccggg ccgaccagct cccgctcaag gttcaccacg
    10021 aacgtcagcg atgcggatat gtcaagcgtc gtcgtatagg gtatgaaacc attctttttc
    10081 agcccgatgg ggcagatacc tgtccgatcg aaggagagcg tcgccggcgt gcgaccatac
    10141 ggctcgttgt tgacagatat atcggccccg gaggggatag agttgaggtg aacggtatag
    10201 agcccgggcg cggcatccga tcggttctcc agcgagcaga ggtagacgtc gtaattgccg
    10261 gtccggttgt cggaccagac gaccttgccg ccgtcgatgt ccggccagac cgggtgggga
    10321 agatcggcga ggatctccgt tccggtttcc gtcgagaggt catacgcaga aatcccattg
    10381 tcgtcgctcc ctctcccgta aacagaccag acgatggcgt cgccggagac cgccggccag
    10441 agatcgtgcc cgggctcatc ggtgatccag gtcgtgttgc ccgatgcgat gtcatagcaa
    10501 cagatgtcat aactcccttc cctccactcc gaccagacga tgtagtctcc cgatatcacc
    10561 tggttccttc gcatgacggg gagatcggtg atccggtcgt gctcgtttga cgtcaggtta
    10621 tagaggtgga tggtatagcc tttcgtcccg tgctgttcag cccagatgat ccgatcgccc
    10681 gatatcgagg gacaggtctc gtcggccgcc gatctggcta tcggattctc gacccccgaa
    10741 gagaggttgc ggtagtagat atccatattt ccgtcccgtt tgtcgtgcca gacgatgatg
    10801 tcgcccgata tggccagggt cgactcccgc tcccatgtta tatcctgcga ctcgctcgtc
    10861 tggtacggca tggcgggata ctgcgtgatc tgggtgattg taccgctccg gatgtcatac
    10921 acgcggatgc acgggaagat cttaccggag gaatcgctct ggtcttccac ccacccgatc
    10981 cagttgccgg agatctccgg ggatgtccgg ggcgaaaagt gctcgatgat catctccgcg
    11041 gatcggtttg caatgtcgta gagatacagg ttcctggtat tgatgcgatc ggtctccatc
    11101 catagtatcc tgttgcctga tatgcgcgga agaacctgat ctctcgtgct gtttgttatc
    11161 tgtacgatcg ggaagttatc tgcccccgga accgcctgta gcgcagggtt tgaggtgtcg
    11221 gcctggaccg catcggcggg ttgcacgctc tggacggtcg cggagatcgc gattacgagg
    11281 aggaaaaatc cggcaatatg gcagatccgg gtcatgccga tcttcaccgg ggcataatat
    11341 gtatcccaga atataatagt atccatatga atgcaggacg atgttccggc gctgtccggc
    11401 ccggtggcgg gcgcttgtgc aggcggcagc gctcccccgg ggaagactgc atgcggccgg
    11461 ctgctgatct gcccgggaag gcgtatgcgg ccggccggta tcctgaggca aggtatttaa
    11521 ccgccaccgc atccacactg cagccgatcg tatgaccctc gtaccaaccc ttcttcgccg
    11581 cgttgtggtg tgctggctgg tgatgttgat cgtgctcgta tcctgttctc tccccgttct
    11641 gggagccgat ggctccgcgg acctgccgcc ggggcacccg ggctcccctc tccggacctc
    11701 cgtggagcac cgaaccgatt atcctttagc ctggcacaac ctgaggccta catggttcat
    11761 gaacgtaccg gacgtccggt ataccgtgcg aaacccgacg gcagagcccg tctgggtccg
    11821 gctcgtatcg gagtacccgg gctacagcgc accgaccgtc agcgtatcgg agatcggcgc
    11881 cgggagtccg gccacgttcg accatttcgt cgtcctggac cgcgaggaga tccaggagat
    11941 ccgggcgccg gccagggtag ccgtccacta ccggatcgag tactgggatg gcggcaactg
    12001 gaccgttcac gacgagcaga cggacccggt cacgttctac ccgatggacc agatggtgtg
    12061 ggccacggag gacaaggacg gggtgatcac gatctatcac gggctcatcg ccatattcgt
    12121 gaccccgcag tcccccgggg tggcagcgct ggcggcaaag gcaaaggagc gggcgaccgg
    12181 ggagttcgac cggaggtatg tggactacgg tatggagcgg acgctgccgg gctatgccct
    12241 tcccaaccaa cggacaacct acgccgatac gaagaatcgg acggcgctgc aggtcaaagc
    12301 catctacaac gcgctgaagt acgactataa cctgagttac gtcgacgcgc tggtagcgtt
    12361 cggcatgggc gactcgcagc gggtcagcac cccggacgag tcgcttgcaa ccgggagcgc
    12421 aaactgtatc gacggtgccg tcctcttcgc ctcggcgata gagcggctcg gtatgcaacc
    12481 gtacatcgtc gtcgtcccgg gtcacgccta cgtcgcatgg aagaccgata aggccgggac
    12541 ggaggtcgat gccctcgaga cgacgtgggt gagttcttgt gattttgaga gggcatacga
    12601 cgcggggaca aagaggtata aggaagatgc taaatccggg aggatggagc tctaccagtc
    12661 gttgttcgac cggaggttgt cgcgcaatga gtacgattcg atctacgttc tcgtggagat
    12721 acggtggctg cggtcccagg gcatcctgcc gatgaagtga ggttttgggg ggccggcggg
    12781 atcgtagaga cggtgccgca ccggggaggg agacggtatg ggcacgaccg caccgcgagg
    12841 cccaaacccc tggcggacag gtctctcctc ctccgtagga gcccgctccc gggcccgatt
    12901 cgcccgcatc ccggcaccgc ccccgcgcac cgcctctccg gcataatcac cccgccaacc
    12961 ggagcgcacc ccggtatccg ctctcaggag gcgttcgttc gtgcccgaac acctccctcg
    13021 accataatgt tgatatacaa aatacattat taataatgta taattggggt aataggatgg
    13081 ctgaaaactt taacgtcagg ctcaatgagg cgaagtacta tactcccgaa gccagctgca
    13141 gggagcagtc ctggataggc gactacaacc gtgcctacca ggcgttcctt gcggaccccg
    13201 atgggttctg ggatagtgcc gccagagaac ttgaatggtt ccagccctgg gaccaggtga
    13261 gagaatggga atacccgcac gccaggtggt ttgtgaacgc aaagctcaac atcacccaca
    13321 actgcctgga ccgccacgcc cacagccagc ggcgaaacaa ggtcgccctc atgtggcggg
    13381 gagagaccga ggacgaggag cgtatcctca cctaccgcca gctcctgcag gaggtctgcc
    13441 ggttcgcaaa cggcctctcc cgccttggcg tcaggaaggg cgaccgggtc tgcatctaca
    13501 tgccggtcgt ccccgagcag atcgtcgcga tgctcgcctg cgcccgcatc ggggccatcc
    13561 acacggtcgt cttcggcggg ttcggtgtca gcgcgctcaa ccagcgtatc cggggcaccg
    13621 ccgcgaaggt agtcatcacc gccgacttca cctaccggcg cggcaaggca atcccgctca
    13681 aaaacatcgt agaggaagcg gtcgtcaaag ccccgagcgt cgagcggatc gtcatcctcc
    13741 ggcgtgacgc cgagaaaccc gtcgagatcc accccgagat ggaagtagac taccacgacc
    13801 tgatgaacgg cgtggggcgc gaatgcccgc ccgagccgat ggatgccgag gatccgctct
    13861 tcatcctcta cacgagcggc accaccggat ccccgaaggg catcgtacac gcctgcggcg
    13921 gctatatggt cgggacctac tacacgacaa agcacatctt cgacgtaaag gacagcgaca
    13981 tctactggtg caccgccgac cccggctgga tcaccggcca cagctacggc gtctacggcc
    14041 cgctcctgaa cggcgccacc tgcctcatca cggagacgac ccccgattac ccgaccccgg
    14101 ccaccaactg ggacctcatc gaggagtacg gcgtcacgat cttctacacc gccccgaccg
    14161 ccatccgcat gttcatgcgg gtaggagaag agtggccgaa ccgctacaac ctctcgtcgc
    14221 tccgcatcct cggatcggtc ggcgaaccgc tcaaccccga ggcgttcgag tggttctacc
    14281 aaaccatcgg gaaaggccgg tgccccatcg tggacacctg gtggcagacc gagaccggta
    14341 tgcagatgct gaccacgacg atcggtgaac cgatgcgccc cgggttcgtc ggaaaaccga
    14401 taccgggcgt cgtcgcggat gtcgtcgaca aaacgggcaa accggtcccg ccgggcaccg
    14461 gcggcctcct ggtcatcaaa gagccctggc cgtccatgat gcggacgatc tggaacgacg
    14521 acgaacgcta ctgcaagtac tgggacacca tcccgggctg ctacacggca gccgacctcg
    14581 cggtgaagga cgaggacggt tacatcatgg tcatcgggcg gtccgacgac ctgatcgtcg
    14641 tcgccggtca caacatcggc accgcggagg tcgagagcgc cctcgtctcc cacgaggccg
    14701 tggcggaggc tgccgtcatc ggaaaacccg acgctctgaa ggggaacacc atcaaggcct
    14761 tcgtcatact cagggacggc cgccagcccg gcgacaagct gaagaacgac ctcacctacc
    14821 acgtccggtt gacgctcggc cccatcgcca taccctccga gatcgagttc gtccggtcgc
    14881 tccccaaaac ccggagcggc aagatcatga ggcgggtctt aaaggcgcag gaactgggta
    14941 tggaccccgg agacatctcg accctggaag agtagacaac caaccccttt atatactctt
    15001 ttatatatgt tataaatatg aatgagcgta gaccagagga atctctcaag atagaaaata
    15061 tcgttgcttc ggcgaaagtg acggattctc ttgatctccc ctcccttgcc tctcagctga
    15121 aggacgcgga atataataaa aaacggttcc ccggtgtcgt tcttcgtatg caggacccga
    15181 agatcgccgc gctcgtcttc ggttccggca aggtcgtcct gacgggcgca aagagcatcg
    15241 acagcctctc gagggggctc gatatactcg ccgaccagtt gcgggcgctc ggcatcgata
    15301 tacccgaaga cctgacctac aagatccaga acatcgtcac gtccgccgac ctcgggacgc
    15361 cgatcaactt gaacaagatc gccgtggggt tcaacctgga caagatcgag tacgaacccg
    15421 agcagttccc cggcctcgtc taccgcctcg acgacccgaa ggtggtcgtc ctcctcttcg
    15481 ggtccggcaa actgatcatc acgggcggca aggtgcccga ggatgcgaag cgtgcggtgc
    15541 agcggatcct ctccgagctc tccggtctcg ggctcatctg aactctggca actactcttt
    15601 tccggattcc gggaatgcgt tctcgggtct ctgatccgta tcccccttct cacacggtgc
    15661 cgccgcaaaa tccgctcccg gccagcacgt gccgccccct gcccgggagg cgcctctctc
    15721 cggcgtatgt tggtcggggt tgccggccac gccctgtccc cgggtattcc ccacactgcc
    15781 cggcaaagcg cgatccgtca gagaaagata tcatcaaccc gatgccctat cggtcttttc
    15841 aggtgcagcg taatcgccgg atctcccatt agaataataa tatttttata gtcagattta
    15901 aaaccataga tactacctga tggtgaacgg atgaggtcag acaaggtacg atactttacg
    15961 ccacgcgacg aagaactcgc cgagctcctc atgggcatcg gtatcaagag aaatgtctcc
    16021 aaagtgctcg tatatcttgc aaacatagat gaagcgacgt cccgcgatat cgagcgcggc
    16081 accgatctcc gccagcccga ggtcagcatc gccatgcgct acctcaagga atgcgactgg
    16141 atcgataccc gcgagagcaa gtcggagagc aaggggcggc cggtgaagat atacgcgctc
    16201 tcccgtccca taaccgagat catggatacg atcgagaagg agaaaaagaa agaagcccgg
    16261 caccagctag acctcatcca gaagatgcgg gaatgtatat caatgcagtg agacgacgcg
    16321 gcaaccgatt ttttcgccga cgataaccgt tatcttttct gtatacgccg taaaaaggcc
    16381 gcactccaga acaccgggga tggcggctat cgctgtttca agcctctcgg gctccccgat
    16441 caccccgaag tcgcagtcga gtatgaagtt gccgttatcg gtgacgaccg ggccgtcttt
    16501 cttcacccct tcccggagga ccggcgcggc cccgagcagg gagaggcgcc gggagacacc
    16561 cgcgagggca aacgggagca cctcgaccgg gacgggcgcg ctcagggtat cgaccgtctt
    16621 ggaggggtcg acgacgatga tcacctcacg ggccgcatcg cagacgcact tctcccggag
    16681 gtgggccgcc ccgcgcccct ttatcaggtt cagggccgga tcgacctggt cggccccgtc
    16741 gatggcgacg tcgagctccg ggtactcgtc gaggccggca agcggtatgc cgtagcggcg
    16801 cgcccggatg gccgcctggt aggatgtcgg tatgccggtg aaagagagcc cctccccggc
    16861 tatccgctcg cccaggcgct ccatcgcaaa gaagaccgtc gaccctgtcc cgaggccgac
    16921 ggccataccg tcctcgacca tcgcggccgc acgataccct gcgttccgct tcggttctgc
    16981 cgtcacgacc tgctcaccat accgtcgaat atgccccgga gctcatccct ggcgccgaac
    17041 gccgtgcagt cgagcgtgat gggcgtgatg gagacgttgc ccttctgcac cgcatgcacg
    17101 tcggtcccct cctccgcatc ctcgtgcagc ggcccgtcta tccagtagta cgggcgcccg
    17161 cgggggtcga agcgctcctc gacgccggtg tagaagagtt tctcggcaag gcgggtgatc
    17221 tcgtagccgc cgcgcaccgg tgtgggtatg ttcacgttga tgacgtgggc gttccccggg
    17281 aacccgtttg cgaggacccg ctcgcagacc tcgcggacca cccttttcgc gtcaaagaac
    17341 ctgtccctga tctgggatgg gtcgtcgaac ttgtcgccct ggtcctccac ctggagagag
    17401 aaagcaaggg aaggcacccc ctggttggat gcctcgaggg cggcgccgac ggtccccgac
    17461 gtcatgatgg actcgtacga gaggttctca ccgatgttga tgccgctgac aacgacatcc
    17521 gggttcaggt gcagggcaaa cagaccgatg ataacggcat cggtcggttt cccgccgacc
    17581 gagtaggccg gcacgccgtt catcagcacc ttcgtcgccc ggatcggctc aaagatcgat
    17641 atcgatcgcc ccacggcgct ctgctgggtg gcgggcgcga cgaccgtgac gtcggcgatc
    17701 ggcgcgaggg cctcataggc cgcccagagt cccgcggacg tgatcccgtc gtcgttggtg
    17761 agcataatct ttggcttcat ccttatctat aggattgttc ccccgtatca aatacttgcc
    17821 cgatcgtcag ggtcaagtgc ctgagaggca acctttactg cgatgaaggt ttttctcgcc
    17881 gaatactcca tctgcaacga tcccggcctt gcgcccgagg gtgccgccat gctcgccgtg
    17941 ctcagcggaa gttttgaacg gtgcggctac gacgtggtga cgccggaggg ccccgacctc
    18001 gaggacggca tacggaggct cgcgccgggg tgcgatgccg gtctggtcat cacgccggac
    18061 cgcctcctct ttcggtatac gcacctcctt gagcagttca cccacaacat cgggtgcggg
    18121 agcatgaatg cggcgatctc tgcaaacaaa cagcgggccg gagcgatcct cgcccggcac
    18181 ggcatagcgg tcccgcccgc tgccggcagc gggagacggg tcgtcaaacc ggtgatgggc
    18241 tgcggggcac aggatgtcag gctgacggac ggggagccgg gcgccgggga gtttgccgag
    18301 gcttacatcg agggggaggc gctgagcgtg agcctcgtgg ggagccgggt gaccggcaac
    18361 gcctgcgagt actactcggg caaccccccg ctcctgcttg ccgtcaaccg gcaggatatc
    18421 gccgtcgcgg acgacgggag tttccagtac ctcgggggag agacgcccat ccaccctgat
    18481 cgggaagagg agatcgtcga tctcgccgtt cgggcgatga acgtcctcgg gtgtcagggg
    18541 tatgccggca tcgacgtcgt cgtcggggac gagatctacg tggtcgacgt caacccgcgc
    18601 cccacgacca gcctggtcgg catcgcggcg gtcatggagg aggagatcgc cgacatactc
    18661 atcagggcgt cgcacggcga ggcgccggcg gacgtgcacc tctccggaag ggtgcggttc
    18721 gataaggacg ggaggatcgc ccgggtatga tcggcatcga cataggcggt gcaaacctca
    18781 agatcgtcga cgacgccggg gtgcacatcc actactgccc gctctggcag ggcgcgccgc
    18841 tcgccggcct cctcgagccc tacgccggtt ccgccgccgt ggtgatgagc ggggagctcg
    18901 cggactgttt ttcgaccaag atcgagggca tccggtggat cgtcgggacc gtccgggagg
    18961 ccatacccga tgccgccttc tacggcaccg atgcggcgtt ccatacccgg ccggtcccgg
    19021 gccttgccgc cgcgaactgg cttgcatccg ccgactacct gcgggaggag tatgccgatg
    19081 cggtcctcct cgacgtgggg agcaccaccg ccgacgtcat ccccctgact cgctttgagg
    19141 acctcaaagg cctgaccgat acccggcggc tgcagaagca gtacctcgtc tacaccggca
    19201 tgctcaggac gaacgtcgcg acgctcctct cgtcggtcac gctcgacggg acggtgaccc
    19261 cggtgagcac cgagtacttc gcggcgagcg cggacgcgca cctcgtcctc ggccacatcg
    19321 cccctgcgga ctacacctgc ccggcgccgg ataacggcga gaagaccgtc gatgccgcgc
    19381 tccggcggct tgcccgggtc gtctgcgccg atatcgacga gatagggaag tccggggctc
    19441 accaggtcgc ccggcagttc tgggaggtcc agcggagggt gatcgggcgc gcggtggggc
    19501 gcgcactctt ccagggcgat gccgaacgag tgatcacggc cggcatcggg gcggacctct
    19561 ttgcccggga actgggctgt gccacgctgg cgcaggagat cggggaggtc tcggatgccc
    19621 tgccggcgta cgctgtcagg gaggtggcgt tacggcgagc cgcctgcgac tgaccctcct
    19681 tttgcttgcg gtgtccgtcg ccgtcacggc agtctcgttc gccctcgggt ttccgttttt
    19741 cttcctcttt ctagttatac cgctgatacc gttcttcgga aagccccctg ccgcgaggcg
    19801 ctgcccggtc tgcgggtggg agaccacagg cggcgagcgc ttctgcccct atgacgggac
    19861 acccctcgcg cccgagggtg gatatggcga ggttcaagag cgagaggggt agaagaaggc
    19921 cgaagagttc ggttgccggt atcgcgtcga agtcgcgcga ggcgtagttc cggatcgtgc
    19981 cgcggtccac gacgatcccc agcgcgcgga ggtgttcggc aacctggttg aagggcatcc
    20041 gctgcgagag gaggaggcag aggtcgacga tgggcgacgc gaggcgggtg tcggggtaga
    20101 aaggtgcatc cgcgtagcag agcgaaccgc actggcggca gtaaaaccgc ttcacaaaga
    20161 ccctgatcgt ccgctcctct ccctcctcaa gtatgacggc aaaccgtttc ggccgcaggt
    20221 cgtgtccggt cacctggccg ccgcacgacg gacaggcgtc gaggtgggtg aatctcacgc
    20281 cgtccatcgc tgtcagggcg ttgcccacga gatcggcgag catcggcgga accggcagtg
    20341 ttttctttgc gatggtaacg ctctcctgtc cagctgtacg aaaagtgtgg gcgtccaccg
    20401 ataataacat attcctccgg ccggcagtat cccggccccc ggttgaacac atatgtctga
    20461 ccggtgcagg tacataaacc cgggatctat ggaaggtcct ccgcgtaaat gtcagacaaa
    20521 gccatacaat ccatttttga cctgccggga ttgttcaggg ggcccgggca gatctggtta
    20581 accactataa tttaagtagc ttaacatcag atattcggtc gccagagagg cgatagatgc
    20641 atgacgttac agtatgttgc tacaacgtgc ccctactgcg gtacgggatg cagtttcaac
    20701 cttgtcgtcc aggacggcaa ggtggttggg accgcaccgt accgccgctc acccgtcaat
    20761 gagggaaagg tctgcccgaa aggcacctac gcccacgagt tcgtgaacag cccggatcgt
    20821 ctcaccaagc cgctcatcaa gaaggacggc aagtttgtcg aggcgacctg ggacgaggcc
    20881 tacgacctga tcgcgcagaa gttcaagtcc tacaagcccg atgagttcgc agcgctctca
    20941 tctgcgaggg tctccaatga agagaattac ctgatgatga agttcgcccg cggcgtcatg
    21001 aagtcgcggc acgtcgacca ctgcgcccgg ctctgccacg catccaccgt cgccggcctt
    21061 gccgcgtcgt ttggctccgg tgcgatgacg aactccatcg gcgacatcgc cgagtccaag
    21121 tgcgtcttcg ttctcgggtc gaacacactc gaacagcacc ccctgatcgg gcgtaagatt
    21181 gtccaggcga agaagaacgg tgcgaaggtc attgttgccg acccgcgcta cacaccgacc
    21241 gcccggctgg ccgacctcta catgccgttt gtctccggct ccgatgtcgc tcttttaaac
    21301 ggcctgatgc aggagatcat cagaaacggc tgggaagaca aagagttcat cgagaagcgg
    21361 acgaaggact acgagaagct caaggaggtc gtcatgaagg agacctacag ccttgagaac
    21421 gtctcgaaga tctccggcat ccctgtcgaa cagctcaagc aggccgccga gtggttcggc
    21481 accaccaagc ccggcgcgat cctctactcg atgggtatca cccagcacac cgtcggcgtc
    21541 gacaacgtcc ggtcggttgc aaacatccag atgctcaccg gcaacctggg taagcccggc
    21601 agcggcgtga acgccctccg cggccagaac aacgtccagg gcgcctgcga catgggagct
    21661 ctgcccgtcg tcttcaccgg ctaccagaaa gtcatcgacc cggccgccta caagaagttc
    21721 gccgacgcct ggggtttccc cggcggtatc tgcgagccga agaacggcta cgaggtcacc
    21781 gtcatgatgg acgtcctgac cgacaacccc ggcgaactca agtgcatgta catcatgggc
    21841 gagaacccga tgctctccga cccggacctc aaccacgtcc ggcacgccct cgagagcgtg
    21901 gagttcatgg tcgttcagga tatcttcctg accgagaccg ccgaacttgc cgatgttgtt
    21961 ttacccgccg catgctacgc tgaacgcggc ggcacccaga cgtccaccga gcggcgcgtc
    22021 cagatgtggc gcaaagccca ggatccgccg ggcgaagcaa aggaagactg gcagatcatc
    22081 agcgagcttg ccgcccgga
//
LOCUS       DAOSII010000018        20568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_2843_length_20568_cov_43.700336, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000018 DAOSII010000000
VERSION     DAOSII010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 20568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20568
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2843_length_20568_cov_43.700336"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1219)
                     /locus_tag="PLV90_02570"
     CDS             complement(152..1219)
                     /locus_tag="PLV90_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96029.1"
                     /translation="MVLEEMIIIRGPIDGPEIARAFREAGMPTRITQTVTLDGRVAAK
                     GTIGDIRALLKGEIARIEKLRKEFLADEEGEEFDAPEDKEGADDREDKEGADDREDEE
                     DADDWEDEYFDTGHLYDPYLDMTECIAEDIARFMEKYRPGDVISMADLKEWMAPLADV
                     APGEERSDALAKSILYKMTALSMLDKNGLIEIVGETVTVKGHMDPEDVIITIPGEAAE
                     EIIPETLKEHNVTMAVTVTPVPEHLLIFGPEAIIDGDLDTIDEIIDDLGIDEEEYISF
                     RAGVSMKRAVIARTLEILEERGTLTPEEALETLNGTPLEGTEEAYSISLELTSEFVKG
                     LLNDLKKVGLVRKKGEKFRAI"
     gene            complement(1250..2425)
                     /locus_tag="PLV90_02575"
     CDS             complement(1250..2425)
                     /locus_tag="PLV90_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF96030.1"
                     /translation="MAGVKVYTTESCPYCRMVQAFLQKHGVEYTIVDVGKEREAAREM
                     VRISGQRGVPVTVFGDEVIVGFDAKKLRELFGTPVADEIYDAVIVGAGPAGLTAAVYC
                     ARKLMKTIVVSEDIGGQAAWSWAIENYMGFRMITGEDLIGKFEDQVRGFDVGLELDRV
                     RGIQKEGDLFFVRASSTYRCRTVILAPGKQPRTLGLPGEDRLIGRGISVCSTCDGPLF
                     RDRPIAIVGGGNAAIQTAVEMTEIASSVALIVRSALRCDEIYIRRAEEAGVRVFPNHE
                     VVELHGDTALAGVTVRDRETGRETALDVEGLFLEIGRIPNTGFLGDLVAQNEQHEILV
                     DENNHTNVDGIFAAGDATCIKGKQIIIAAGEGAKAALEAHEYLIKKGLSRAPLSATL"
     gene            complement(2521..3021)
                     /locus_tag="PLV90_02580"
     CDS             complement(2521..3021)
                     /locus_tag="PLV90_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOF96031.1"
                     /translation="MPTDENAQEAFAGESQANRKYSVFAEKATAEGYPVVGKLFRAAS
                     EAEAVHAKRLLFIKNAVGSTEENLKGAIEGENYEFMTMYPTFAEEAKKERRNEAAIVF
                     THAMKAEEVHANLYLQALEAVREGKDIDVEKIYLCPVCGNVELGAAPEKCPICGVPAR
                     MFREVQ"
     gene            3110..3976
                     /locus_tag="PLV90_02585"
     CDS             3110..3976
                     /locus_tag="PLV90_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOF96032.1"
                     /translation="MQGDRVQRVFVIVLILNVAVAVVKAVFGVMAGSVSMVADALHSG
                     FDSFSNVVGIVAMHLAGKPPDPEHPYGHGKIETLGTLVIGAMLLLTAAGILLEGYGRL
                     ITPITPDITPITVGVMVGTLITNIIVSTYERRKGEEYQSQILIADSLHTRSDVFVSTA
                     VLGGFLAIRLGYPAADPIIALGIGLLIARMGVGILYGAAEVLIDSMNLPCDPALVRAV
                     VMDTPGVAGYHDFRCRGKPGEIFADIHVTVDPALSIARAHAISEEVEQRLKETVPGLA
                     EVVVHIEPDDSA"
     gene            complement(3968..4564)
                     /locus_tag="PLV90_02590"
     CDS             complement(3968..4564)
                     /locus_tag="PLV90_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOF96033.1"
                     /translation="MGRKVIALLGSPLPDGNTARLLDEAIRGAEEAGCDVERIVAAHL
                     DIAPCMEIFYCQENETCEIRDEMIGIYDKFREMDGLIIATPVMTMGIPGRLKSFMDRF
                     QVFYMAKYHRGRSFISPEQRRRRKMLFISIAGMNLPTVFEGAKMTAKAFGEIIDCPYW
                     DEVLRNDMDTIQDIRTRPEVMEAAYRKGYELGQLLSSR"
     gene            4692..4922
                     /locus_tag="PLV90_02595"
     CDS             4692..4922
                     /locus_tag="PLV90_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96034.1"
                     /translation="MAEPGTRTTAKAGEKPGPGRFICIDCGKEVRIGSTDEDLVKCPT
                     CACEMYNCLPMTHIRPDIKTPEDVMNPPERKK"
     gene            4948..5073
                     /locus_tag="PLV90_02600"
     CDS             4948..5073
                     /locus_tag="PLV90_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin FeS4 iron-binding
                     domain-containing protein"
                     /protein_id="HOF96035.1"
                     /translation="MVNVSAKGEIYRCEICGNVVEVLEAGGGELICCGEPMVLEE"
     gene            5079..5435
                     /locus_tag="PLV90_02605"
     CDS             5079..5435
                     /locus_tag="PLV90_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOF96036.1"
                     /translation="MAEEQKNLEEKIGKIPEIFKELKETDPDLYGKVMGLDQMIWADG
                     ALSKQTKKVIAIAIAAALRDGHAVRAQMAGAGNLGVSKEEIEEGLRVAFLLAGMPAYV
                     HGKTALEEYLGSRPRK"
     gene            5440..6084
                     /locus_tag="PLV90_02610"
     CDS             5440..6084
                     /locus_tag="PLV90_02610"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOF96037.1"
                     /translation="MEPEGLPQMPVIGEKAPEFDALTTHGPLKLSDLAGKWVILFSHP
                     ADFTPVCTTEFIAFAGMADELAKLNVQLVGLSIDSVHSHLAWVRNIKEKMGVDIPFPI
                     IADLDMKVAKRYGMLHPGQSSTSTIRTVFFIDDKGIMRAMLYYPMSSGRSMPEILRLT
                     KALQTADKHHIATPANWEPGDKVIVPAPKTPKEIEKRMQEGYECKDWYLCFKKL"
     gene            6126..6737
                     /locus_tag="PLV90_02615"
     CDS             6126..6737
                     /locus_tag="PLV90_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOF96038.1"
                     /translation="MTIDVLAFATSPRRHGNSETLLDWMLDAMKEEGAAVEKIVVPEV
                     DIRPCRGCNACAKLNRCIQRDYMDYVHDRIVEADCIILASPIYCMGLTAQAKVLVDRA
                     QVFCTRKYVLRLPVVPPERKGKRVGVFLSTAGQNWDHVFDAAIPSVKCFFNVIDVRDK
                     DIRYLMVNNVDEKGAIARHPTARADAAALAGEVAAYLQEVGVA"
     gene            6734..7360
                     /locus_tag="PLV90_02620"
     CDS             6734..7360
                     /locus_tag="PLV90_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOF96039.1"
                     /translation="MTDSVRVLGISGSPRRHGNTETLLDAVLEGTREAGADVEKIVLR
                     SLAYTPCRGCNVCHKTGVCIIEDDLTEIFDKIAAADVLIIASPIYSMGITAETKGLID
                     RAQYLWARKFILKTLYFTTEQTRRHKGIFVSTAGLGWGNVFDAAFPAITAFFNTTGFE
                     YWDNVIANDLDRYGGIAGHPTALAAGREKGREVVALIRKMQAREGTET"
     gene            7595..10540
                     /locus_tag="PLV90_02625"
     CDS             7595..10540
                     /locus_tag="PLV90_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF96040.1"
                     /translation="MPLELRLLDGIDDWLLLIGRDHNVIRVNSAIRRIHGALEGDLDQ
                     AVCALGGSRGLAGRIRTALDDEDEIPGFECRLQGPDGGERRYLCSGWRMPSGEGWLLR
                     FREIPVAGDYEAVVEHTGTATILIEDDGTISLANTEFERLSGYSRREIVGVKRLTDFI
                     ASEDECRQIREYHILRRSNPAAAPKNYSFSFIDRAENVHIVEATIGMMPGTARAVMSL
                     INVTGRRRAEQALQESEERLNLAFLAANDGLIDWDVRTGKIFYSPRSFTMLGYEPGAF
                     VPTYPMILTLVHPEDRDRVGEALADLTSGRRDRCDMEFRVRSASGDFVYVLDRLQVVS
                     HGAGGEPLRIVGTHSDITERKEAERELRIKDMAIASSLDAISLTDLEERITYANRAFL
                     TMHGWDREEIIGQPASVLWADPEEAKRVIAEFLRDGTWSGEVAGRRRDGSTVHMQLAL
                     STITDEAGARLGSMGSGIDITRRKQAERELLIRNMAIASSLTAFAITDLDGRLTYANQ
                     AFFSLWGYTSAAEVVGRRVTGFWQDEGEAEDAVRMLLETGRYTGERVGRRRDGAKFYA
                     MFSGNLVRDEAGAPICLAASLTDITDLKRAEAEIQAHNRELSVLNQIIGVSTSATGID
                     EALGDMLAATIALLDLRGGGVYLVEADRQRARLICTRGLPDGFPLQTCIPDITAPPYA
                     DVLVAGEPFFFDDPTHLRYPGEEREMLHPYASIPIVASRGIIGSLNVVPRDRRRFTDA
                     DRSLLLAIGKEVGSSLECILLIRQLESAEREANLYLDILSHDIRNAENISSLYIDILI
                     DMLEGDAKDHAQKIRSSIRKSIEILRNVSTIRRIHHESVTLAPVDLDEVIRDEIGVFS
                     EINFNYEGTDHAVMADPLLPEVFTNLIGNAVKFGGSAVEITIRVEESGNDVLISVEDT
                     GPGISDEMKDTVFMRFGKNRIQKSGQGLGLSIVRMLVERYGGTIRSDDRVSGCPGCGA
                     AFRFTLKRA"
     gene            complement(10556..11140)
                     /locus_tag="PLV90_02630"
     CDS             complement(10556..11140)
                     /locus_tag="PLV90_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50"
                     /protein_id="HOF96041.1"
                     /translation="MLERIPLRERRDLLIAWLAISIAFTLIYIRGGVNVTGLIFFFVM
                     SLLTVGVAFILHELAHKFTAMRYGYWAEFKKDNQMLLVAVVMAALVGVVFAAPGATYI
                     YGNATRAENGRISAAGPLTNLLLCIPFALLMLFGGGLIGIVGLVGFRVNAMIATFNML
                     PISVLDGRKILAWNPAVFAVLMAASAGLLIWSLL"
     gene            11242..11526
                     /locus_tag="PLV90_02635"
     CDS             11242..11526
                     /locus_tag="PLV90_02635"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOF96042.1"
                     /translation="MSLDAVRSEIRGIDEKIIDLIAERQRLAARIARFKQEEGLPIRD
                     GPQRKAVLDRVFAHAAESRIDPVAVRNIFEILIDMNEERQRECSGDGNLP"
     gene            complement(11536..12405)
                     /gene="argB"
                     /locus_tag="PLV90_02640"
     CDS             complement(11536..12405)
                     /gene="argB"
                     /locus_tag="PLV90_02640"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOF96043.1"
                     /translation="MKREDVLMEALPYIQKFYGRTIVIKLGGHAMVDQNILETVIRDA
                     VLLRYVGMKVVLVHGGGPEITAKMQAMGKEPRFVGGLRITDAETLEIAQMVLVGKIND
                     GIVSLIANCGTRAVGISGNDGNLLIARKMEPQRVRVGEVEEEVDLGRVGEIEEVDPEV
                     LHCLLAQNYIPVVAPIAIDRQGGSLNINADTAAAEIAIALQAFKLINLTDVDGVMNAD
                     RTMTYHRLTLSEADTMIAEGIISGGMIPKLDGCMRAVRSGVTSAHVVNGNREHNLLLE
                     LFTDEGVGTMITL"
     gene            complement(12402..13541)
                     /gene="argJ"
                     /locus_tag="PLV90_02645"
     CDS             complement(12402..13541)
                     /gene="argJ"
                     /locus_tag="PLV90_02645"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HOF96044.1"
                     /translation="MKSICAVKGVSASGIKEGKFGLALIRAGGTAAGVFTSNKVRAAP
                     IEVMMKRMERGTLDAVAVNSGCANAYTGERGCRDALEMCDIAAETLKLAPESVGVAST
                     GVIGRYLNLPLIRDQCRRVAPLLARNGDAEDAAARAIMTTDTFPKHALVRTESFAVGG
                     ITKGSGMIAPNMGTMLAFIYTDAEVEAPALRDILREATRRSFNRVVVDGDTSTNDVAL
                     CTATGTAGRVDRDELARAIEAVCRSLAIQIARDGEGATKLLEVTVRGAADEDEAAGVA
                     RTIVASPLVKTAIYGEDPNWGRVVAAAGRAGAEFDPYAVSLSVGGVPLVVRGEIVADL
                     GAAKKAMHGDTVAFDLDLAAGDGMATAWGCDLTEKYVEINGKYTT"
     gene            complement(13538..14014)
                     /locus_tag="PLV90_02650"
     CDS             complement(13538..14014)
                     /locus_tag="PLV90_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOF96045.1"
                     /translation="MQVKDIMTPDPVTVRVDSPIREAAGLLRKYHIGGLPVMDGDRVA
                     GIVTETDIISLLDTGSLSSDLWLPSPLEVIEVPVREFINWEKTKRALTDIGDREVRRV
                     MSSPAVTIDEESDIADAASLMLRERIARLPVIRNGKLIGIVTRADIVRGIGEGEGA"
     gene            complement(14028..15014)
                     /gene="argC"
                     /locus_tag="PLV90_02655"
     CDS             complement(14028..15014)
                     /gene="argC"
                     /locus_tag="PLV90_02655"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOF96046.1"
                     /translation="MEIAIIGASGYTGGELMRLLLNHPSAEVVAATSRKLDGTPVASV
                     HPHLRGLTDLAFRNIGAGDIDADFVFLAVPHTAAMKVAGTLVERGIRTVDLSADYRLS
                     KEIYEKTYGVTHEAYFEAPYGLTELHRDEVRGASFVANPGCFPTGATLAAAPLAHLAH
                     TIIYDSKTGVTGAGNTPSATTHYPNVGDNFSAYKWTSHRHLAEMKQEAARLGSTARCY
                     FTPHLLPVNRGILTTAHILLNEPMESKEVETLYRKFYEREFFVRYQKPTLAAVRGTNF
                     CDVAVESEGDRVVAVSTIDNLVKGASGQAIQNMNLMCGFAEGAGLCFAGMSP"
     gene            complement(15041..15946)
                     /locus_tag="PLV90_02660"
     CDS             complement(15041..15946)
                     /locus_tag="PLV90_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOF96047.1"
                     /translation="MLDQFKGCLLGAAIGDALGMARESTPPDFQRLHEGYRRAWRGHP
                     NAGLKPGQFTDDTQMMLLVAGMLADGTYSESAYAAALARMYMNEELRFPDGAVDAACR
                     HLLLSGEKSVGVASNTAGCISIAIPFALAYAEPVDVTERVVQACSVTHTHPAAHAGAV
                     TVGMLVHYAVRGRSDALTLAGKHAGLEDVTLGNKIRDAVRLANEGISLESALSVIGND
                     VTVYQTVPLAFFLIHRIRDASALLNTAAHVGGNTDTIALICGAYIGAVYGKTALPGDL
                     LEGLECRSEIESVAARLYERCTTKP"
     gene            complement(16036..16350)
                     /locus_tag="PLV90_02665"
     CDS             complement(16036..16350)
                     /locus_tag="PLV90_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96048.1"
                     /translation="MREKQFTNCIGQKRLICVALLLFILIGSGIAHAATDPGEDVQAN
                     PTDGAAPSAALAEPADTTAYQGSNDVFSFFYNPVLLMCALGAVIVLLAGYVLVRKSRG
                     KA"
     gene            16999..17172
                     /locus_tag="PLV90_02670"
     CDS             16999..17172
                     /locus_tag="PLV90_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96049.1"
                     /translation="MEEIERIGKTYGFPIHLTIYPREVFDRDLHTDPFLREVLRNHVL
                     IKGAEHFFEKAVR"
     gene            17169..17309
                     /locus_tag="PLV90_02675"
     CDS             17169..17309
                     /locus_tag="PLV90_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96050.1"
                     /translation="MTDRLVWCNRIKNGISLSAPNETLASAYLKKAEEALETMQTITA
                     RD"
     gene            17409..17642
                     /locus_tag="PLV90_02680"
     CDS             17409..17642
                     /locus_tag="PLV90_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96051.1"
                     /translation="MERLLSDYFTPAETALVEKARGARIDAWYYVSREVPDPFCEELI
                     WAAPRFLVKCGGIADGMNEKTIETLRGALRKEE"
     gene            complement(17862..18761)
                     /locus_tag="PLV90_02685"
     CDS             complement(17862..18761)
                     /locus_tag="PLV90_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96052.1"
                     /translation="MMNGIKVRLFGLLLASLLIGAFFVPVISAGGESTNWLDTFDERV
                     TPRGMSTYNKPIVTKEPEQPGPEPAEIHPGLVLLGENVTFPDAGYVAASGLPSGPTAH
                     VTVADPAYFSPVPSAGEVNLTNLPPLRKYDLVTADPAAFVAGAGSGSPVTLRLPDREF
                     VLDLEPVPGPVAEGARAFVKNESGTFEVALPHIWRFEGTVVGDPGSSASFTVGGDAIL
                     GTIRCGPTLLVIGQAGTVEIDGEQRVVHVIYDERDITPRLRSPIFDCCVPPAGARDSP
                     DITENRITSLVKSLIDAIATGRT"
     gene            complement(18770..19582)
                     /locus_tag="PLV90_02690"
     CDS             complement(18770..19582)
                     /locus_tag="PLV90_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-dependent protein kinase"
                     /protein_id="HOF96053.1"
                     /translation="MFSLTAEFPEMRDSYTLYRTVPVTVTEEQVREVAERFGLAGEPG
                     TMNKKTGEFLLIDASKDPEEQVSVYAHSGAVAYHIPDLELPTEAIRQPDLPTDAEAER
                     IALAFLEKTGMQSPGARVVKIGVGQKQEVWKAGESEPEVSCDVTKTVRFGRPLDGLPV
                     YGDEFTVILGDGGKVAGLVKTWREVTPDGNISLRSPGEAYEDLLAARTIRPRVGGEYG
                     RITIENVSIGYWMEPRGVVQDQVRPVYAFSGTAFRDGVPEPYIGYVFADAGG"
     gene            complement(19710..>20568)
                     /locus_tag="PLV90_02695"
     CDS             complement(19710..>20568)
                     /locus_tag="PLV90_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96054.1"
                     /translation="RARWHPGCVPGEAPTPSLWWKIRMKSLKTDRTSKIAFILLLITI
                     GAFGILLLVDLLAIGPGYPPPKALQKWYIPQPRYEYAENGSVAANRTIDGNVVLLGDI
                     EDGCPSLFPVLSRHCSHAMYVDTMSGDRYLVVNWYFDDKTDLLQAETVLCIHLRASGN
                     VSSAEMFLSREPELSRGEPVFSPVAVTKFDNKTSSGYLAVVEKPLSPKHDDYFIVYYG
                     VVGPAILPDHTATLENLMLRSYSFHGARPLAPCSAREAATGSMHSVCEGMPLPPRAAE
                     STAASNQRF"
BASE COUNT         3917 a         6429 c         6253 g         3969 t
ORIGIN      
        1 catccatggc agggaatcgt ctccccggga gataagggtg cctctcccgg gagcggacgt
       61 tacgcccgca attatcatgg agctctatgg ccggggcctt tccccggcag tccgcccggt
      121 tcccggagcc gttcacaggc ccgtatcgtg tctaaatcgc gcggaacttc tcgcccttct
      181 tgcgcaccag tccgaccttc ttgaggtcgt tgaggagccc tttcacgaat tcggatgtga
      241 gctccagcga tatgctatag gcctcttcgg tgccctcaag aggagtgccg ttgagcgtct
      301 ccagggcctc ctcgggcgtc agcgttccgc gttcttcgag gatctccagg gttcttgcga
      361 tgacggctct cttcatggaa acacccgccc ggaacgatat gtactcctct tcatcgatcc
      421 cgaggtcgtc gatgatctcg tcaatcgtat cgagatcccc atcgatgatg gcctcaggac
      481 cgaagatcag caggtgttcg ggcaccggcg tgacagtcac cgccatcgtg acgttgtgct
      541 ctttgagagt ctccggaata atctcctcgg cggcctcgcc cgggatggtg atgatgacat
      601 cctcaggatc catatgcccc ttcacggtca ccgtctcacc cacaatctcg atcaggccgt
      661 ttttatccag catggagagc gccgtcatct tgtataatat ggacttcgcc agggcatccg
      721 agcgttcctc tcccggggca acatcggcga gtggcgccat ccactctttg aggtcggcca
      781 tagagataac atccccgggc cggtacttct ccatgaaccg ggcgatatcc tccgcgatgc
      841 actccgtcat atcaaggtag gggtcataca gatgcccggt atcgaaatat tcgtcctccc
      901 agtcatcggc atcttcctcg tcttcccggt catcggcgcc ctccttgtcc tcccggtcat
      961 cggcgccctc cttgtcctcc ggggcgtcga actcttctcc ctcttcatca gccagaaatt
     1021 ccttgcggag tttctcgatt cgcgctatct ctcccttcag cagcgccctg atatccccga
     1081 tcgttccctt cgcagcaacc cggccgtcca gggtaaccgt ctgcgtgata cgggtcggca
     1141 taccggcctc cctgaaggcg cgcgcgatct ccgggccgtc gatagggcca cggatgatga
     1201 tcatctcttc gagtaccata cgtcttcccc acactattga cgtacctgct cataaggtcg
     1261 ccgataaagg agcgcgagaa agtccctttt tgatgagata ctcgtgtgct tcaagcgccg
     1321 ccttcgcccc ttcgcctgca gctatgatga tctgcttgcc cttgatgcag gtcgcatcgc
     1381 cggccgcaaa gattccgtcg acgtttgtgt gattgttctc gtcgaccagg atctcgtgct
     1441 gctcgttctg cgccaccaga tccccgagga accccgtatt cggtatacgc ccgatctcaa
     1501 ggaagagccc ctccacgtcg agagccgtct ccctcccggt ctcgcgatcg cgaaccgtga
     1561 cgccggccag ggctgtatcg ccgtggagct ccaccacctc gtgattgggg aagacccgca
     1621 cacctgcctc ctctgcccgc ctgatataga tctcgtcgca ccggagagcg ctcctgacga
     1681 tcaacgccac ggaacttgca atttctgtca tctcgacggc tgtctggatc gctgcattcc
     1741 ccccgccgac gatggcgatg ggccgatcac ggaagagcgg gccatcgcag gtcgagcaga
     1801 cggagatacc tcttccgatc aaccggtcct cacccgggag ccccagggtc cggggctgtt
     1861 tcccgggggc gaggatgacc gtccggcaac gataggtgct ggacgcccgg acaaaaaaga
     1921 ggtcgccttc cttctggata ccccggaccc ggtcaagctc gagaccgaca tcgaacccgc
     1981 ggacctggtc ctcaaacttc ccgataaggt cctcccccgt gatcatccga aatcccatat
     2041 agttctcgat tgcccagctc caggctgcct ggccgccgat atcctccgat acgacgatgg
     2101 tcttcatgag ttttctcgca cagtagaccg cggccgtcaa ccctgccggc cccgcaccca
     2161 cgatgaccgc atcatagatc tcatccgcca ccggcgtccc gaagagttcc cggagcttct
     2221 tcgcatcgaa cccgacgatc acctcatcgc cgaagaccgt gaccggcacg ccccgctgtc
     2281 ccgatatccg gaccatctcc cgcgccgcct cgcgttcctt cccgacatcg acgatcgtgt
     2341 attcgacacc gtgcttctgg agaaacgcct ggaccatccg gcagtaggga cagctctctg
     2401 tcgtgtagac cttcacgcct gccataagga aaggatagtc cgggagctct atttaatctg
     2461 tggggtctgg ccgggcagat cgcggatgtc cggccggagc gatacggggc aaaaaaaggg
     2521 ttattggacc tcgcggaaca tccgcgccgg gaccccgcag atggggcact tctccggcgc
     2581 cgctccgagc tcgacgttgc cgcagaccgg gcagagatag atcttctcca cgtcgatatc
     2641 ttttccctcc cggacagcct caagtgcctg gaggtagagg tttgcgtgca cctcttcggc
     2701 cttcatggcg tgggtgaaga cgatcgcagc ctcattcctc cgctctttct tcgcctcttc
     2761 agcaaacgta gggtacatcg tcatgaactc gtagttctcc ccctcgatcg cgcctttcag
     2821 gttctcttcg gtgctcccga cggcgttctt gatgaagagg agacgttttg cgtggacggc
     2881 ttctgcctcg cttgccgccc ggaagagttt cccgaccacc gggtaacctt cggcggttgc
     2941 tttctcggca aagaccgagt atttcctgtt cgcctgggac tccccggcga acgcttcctg
     3001 agcgttctca tctgtcggca taacgaatta tttcggaggt gccccgttaa atatctttca
     3061 cccggcgccc ccggaaaccc tatgccgcgg gagcgactat taccggtata tgcagggtga
     3121 ccgggtgcaa cgggtcttcg tcatcgtcct catcttgaat gtcgccgttg cagtagtaaa
     3181 agcggtcttc ggcgtcatgg caggatcggt gagcatggtg gcggatgctc tccactcggg
     3241 attcgactcc ttctcaaacg tcgtcgggat cgttgccatg caccttgcag ggaaaccccc
     3301 cgacccggag cacccgtacg gccacggcaa gatcgagacc ctcgggaccc tcgtcatagg
     3361 ggcgatgctc ctcctgaccg ctgccggcat cctccttgag gggtacgggc gcctcattac
     3421 ccccatcact cccgatatca cccccatcac cgtcggggtc atggtcggga cgctcatcac
     3481 caacatcatt gtctccacct acgagcggcg gaagggcgag gagtaccaga gccagatcct
     3541 catcgccgac tcgttgcaca caaggagcga cgtcttcgtc tccaccgccg tcctcggcgg
     3601 gttcctcgcc atcaggctcg gctaccccgc ggccgacccg atcatcgcgc tcggcatcgg
     3661 cctcctcatc gcgcgaatgg gtgtcggaat cctctacgga gccgcggaag tcctcatcga
     3721 ctcgatgaac cttccctgcg acccggcgct cgtccgggcc gtggtgatgg atacccccgg
     3781 tgtcgccggt taccacgact tccggtgccg gggtaaaccg ggcgagatat ttgccgacat
     3841 ccacgtcacc gtcgacccgg ccctctccat cgcccgggcc cacgcgatct ccgaggaggt
     3901 ggaacaaagg ctcaaagaga cggtgccggg actcgccgag gtcgtcgtcc acatcgaacc
     3961 ggacgattca gcgtgaggag aggagttgcc cgagctcgta acccttccgg taggccgcct
     4021 ccatcacctc cggccgcgtc ctgatatcct ggatggtgtc catatcgttt cggagcacct
     4081 catcccagta ggggcagtcg atgatctctc caaacgcctt cgcggtcatc ttcgcgccct
     4141 caaagacggt cggaaggttc atccccgcta tcgatatgaa gagcattttg cgcctcctcc
     4201 tctgttcagg ggatataaag gacctgcccc ggtgatactt cgccatgtaa aagacctgga
     4261 accggtccat gaacgacttg agcctgcccg ggatccccat cgtcataacg ggagtggcga
     4321 tgatcaggcc gtccatctcc cgaaatttgt cgtatatccc gatcatctcg tccctgatct
     4381 cgcaggtctc attttcctgg cagtaaaaga tctccataca gggcgcgata tccaggtgtg
     4441 cggcaacgat ccgctcgacg tcgcacccgg cctcctccgc cccccggatg gcctcatcga
     4501 ggaggcgagc ggtgttcccg tcggggagcg ggctcccgag aagagcaata accttccttc
     4561 ccatgatgct gggttacaca cggccctatt aataagatgc ggctctccgg ggcccgggag
     4621 cccgctccgg ccttgcccgt gaggatactg ctaaatactt tgaaacgata tgatcgctct
     4681 ggtgagtgcc tgtggccgaa ccaggaacca gaacgacagc aaaagcagga gagaagccgg
     4741 gccccggacg gttcatctgc atcgactgcg ggaaagaagt ccggatcggc agcaccgacg
     4801 aggatctcgt caagtgcccg acctgtgcct gtgagatgta caactgtctt ccgatgaccc
     4861 acatcaggcc ggatatcaag acgccggagg atgtcatgaa tcctcctgaa aggaagaagt
     4921 gaccggaaaa ctatcaaggt gagagatgtg gtgaacgtat cagcaaaagg tgagatttac
     4981 cgctgcgaga tctgcggaaa cgtggtcgag gtcctggagg ccggcggcgg cgagctgatc
     5041 tgctgcggcg agccgatggt gcttgaggag tgacggacgt ggcggaagag cagaaaaacc
     5101 tcgaagagaa gattggaaag atccccgaga tcttcaaaga gctcaaagag acggatcccg
     5161 acctttacgg gaaggtgatg gggctcgatc agatgatctg ggccgacggc gccctctcaa
     5221 agcagacgaa gaaggtcatc gcgatcgcga tcgccgccgc gctccgggat gggcatgccg
     5281 tccgcgcaca gatggccggc gccgggaatc tcggcgtatc gaaggaagag atcgaggaag
     5341 gactcagggt cgcgttcctg cttgcaggga tgccggccta cgtccacggc aagaccgccc
     5401 tcgaagagta tctgggcagc cgcccgcgga agtgatacga tggaaccaga aggattaccc
     5461 cagatgccgg ttatcggcga gaaagcaccc gaattcgacg cattgaccac ccatggacct
     5521 ctgaaactct cggacctcgc aggaaagtgg gtcatcctct tctcccaccc ggccgacttc
     5581 acgccggtct gcaccacgga gtttatcgca ttcgccggga tggctgacga gcttgcgaaa
     5641 ctgaatgtcc agctcgtcgg cctctcgatc gacagcgttc actcgcacct tgcgtgggtc
     5701 aggaacatca aggagaagat gggcgtcgat ataccgttcc ccatcatcgc cgacctcgac
     5761 atgaaggttg caaagcgtta cgggatgctc caccccgggc agagcagcac atcgaccatc
     5821 cggacggtct tcttcatcga cgataagggc atcatgcgtg ccatgctcta ctacccgatg
     5881 tccagcggcc gctccatgcc cgagatcctc aggctcacga aggccctcca gaccgccgat
     5941 aagcaccata tcgcgacgcc ggcgaactgg gaacccggtg acaaggtcat cgtcccggcc
     6001 ccaaagacac ccaaagagat cgaaaagcgg atgcaggaag gctacgagtg caaagactgg
     6061 tacctctgct tcaaaaaact ttaaatccga cacttttttg ccttcagccg gacagtactc
     6121 actgtatgac cattgatgtc ctcgcgtttg cgacatcgcc ccgccgccat ggcaactccg
     6181 agaccctgct cgactggatg ctcgatgcga tgaaagagga aggggcagcg gtcgagaaga
     6241 tcgtcgtccc ggaagtcgat atccggccct gccgggggtg caacgcctgc gcgaagctca
     6301 accggtgcat ccagcgggac tacatggact acgtccacga ccggatcgtc gaggccgact
     6361 gcatcatcct cgcgtccccc atctactgca tggggcttac ggcgcaggcg aaggtgctgg
     6421 tcgaccgtgc gcaggttttt tgcacccgga agtacgtgct tcgcctcccg gtcgttcccc
     6481 cggagcggaa gggaaagcgt gtcggggtct tcctctcgac cgccgggcag aactgggacc
     6541 atgtctttga tgcggcgata ccgtcggtga agtgcttctt caacgtcatc gacgtcaggg
     6601 ataaggatat ccggtacctg atggtgaaca acgtcgacga gaaaggagcg atcgcccgcc
     6661 atcccaccgc gagagcagac gccgcggccc tggccgggga ggtcgccgcg tacctgcagg
     6721 aggtcggtgt ggcatgaccg attccgtgag ggtcctcggg atatccggga gcccccggcg
     6781 gcacggaaac accgagaccc tgcttgatgc cgtcctcgag gggacccggg aggccggggc
     6841 cgacgtggag aagatcgtcc tccggtcgct tgcctacacc ccatgtaggg ggtgcaacgt
     6901 ctgccacaag accggggtct gtatcatcga ggacgacctc acggagattt tcgataagat
     6961 cgccgccgcc gacgtcctca tcatcgcgtc tcccatctac tcgatgggga tcacggcgga
     7021 gacgaagggg ttgatcgacc gtgcccagta cctctgggca cgcaagttca tactgaagac
     7081 cctctacttc accaccgaac agacccgccg tcataagggg atcttcgtat cgactgcggg
     7141 gctcgggtgg gggaacgtct tcgatgcggc gtttcccgcc atcaccgcct tcttcaacac
     7201 caccgggttt gagtactggg acaacgtcat agcaaacgat ctcgaccgct acggcgggat
     7261 tgcgggccac cccaccgcac tcgcggcagg ccgggagaag gggcgggagg tggtcgctct
     7321 catccggaag atgcaggcgc gagaaggcac ggagacctga tcccctccgg gtcgggtggg
     7381 catcaatact atttccccgg ctgccggatc ccgatatatt ccagcagggt cccgatgtga
     7441 agcgcgggta atattttccc actcattcgg gatcgaagtg tattaatacg gctatggtaa
     7501 gataacaccc ctgtaactac ccccgagatg cttttcaggg ccacagaagg atacgcaggt
     7561 gactcctatc aaaccccggg aaacccctga tgaggtgcca ctggaattac ggttacttga
     7621 cggcattgat gactggcttc tcctgatcgg cagggatcac aacgtaatcc gggtaaactc
     7681 ggccatccgc cgtatccacg gggcactgga gggcgacctc gatcaggctg tctgcgcact
     7741 cggcgggagc agaggtcttg ccgggaggat ccgcaccgcg ctcgatgatg aagatgagat
     7801 ccccgggttt gaatgccggc tccagggccc ggacggaggg gagcggcggt atctctgctc
     7861 ggggtggagg atgccgtccg gggagggctg gctactccgg ttccgggaga tccccgttgc
     7921 cggggattac gaggccgtcg tcgagcatac cgggacggcg acgatcctca tcgaggacga
     7981 cggcaccata tcgcttgcga acacggagtt cgagcggctc tccgggtact cgcgccggga
     8041 gatcgtcggg gtgaaacggc tgaccgattt cattgcctcc gaggacgagt gccggcagat
     8101 cagagaatac catattctcc ggaggagtaa ccccgcggcg gcgccgaaga actactcatt
     8161 ctcgttcatc gaccgggccg aaaacgtcca tatcgtcgag gccaccatcg gcatgatgcc
     8221 cgggacggcc cgggcggtta tgtcgctcat caacgtgacc gggcggaggc gtgcggagca
     8281 ggcgctgcag gagagcgaag agcggttgaa ccttgcattc ctggcggcaa acgacggact
     8341 gatcgattgg gacgtccgca ccggcaaaat cttttacagt ccgcgcagtt ttacgatgct
     8401 cggctacgaa ccgggagcgt tcgtgcccac gtaccccatg atccttaccc tcgtccatcc
     8461 tgaggaccgc gatcgtgttg gggaggcgct cgccgatctt acctccggcc ggcgcgaccg
     8521 ctgcgatatg gagttccggg tgcggtcagc atccggggac tttgtctacg tgctcgaccg
     8581 actccaggtg gtcagccacg gtgccggcgg agagccgctc cggatcgtgg ggacgcattc
     8641 cgatatcacg gagaggaaag aggcggagag agaactccgg atcaaggata tggcgatagc
     8701 gtcatcgctc gatgccatct ccctcaccga tcttgaagag aggatcacct acgccaaccg
     8761 tgctttcctc accatgcatg ggtgggatcg ggaggagatc atcgggcagc cggcttccgt
     8821 gctctgggcc gatccggagg aggcaaaaag ggtcatcgcg gagttccttc gggacggcac
     8881 ctggtccggg gaggtggcgg ggcgacggcg ggatgggtcg accgtccata tgcaactcgc
     8941 cctctccact atcaccgacg aggcaggagc acggctcggt tcaatgggtt ccgggatcga
     9001 tatcacccgg cgaaaacagg ctgaacggga acttctgatt cgcaatatgg cgatagcgtc
     9061 ctcgctcact gcattcgcga tcacggatct cgacggccgc ctgacctacg ccaaccaggc
     9121 gttcttctcc ctgtggggct acacctccgc tgctgaggtc gtcgggaggc gggttacggg
     9181 gttctggcag gatgaagggg aggcggagga tgccgtccgg atgctccttg agaccggcag
     9241 gtataccggc gaacgggtcg ggaggaggcg ggacggggcg aaattttatg ccatgttctc
     9301 cggaaacctg gtcagggatg aggccggagc acccatctgc cttgcggcct ccctcaccga
     9361 tatcaccgat ctgaaacgag ccgaggcgga gatccaggcc cataaccggg agctctcggt
     9421 cttgaaccag atcatcgggg tatccacatc cgcgaccggg atcgatgagg cactcgggga
     9481 tatgctcgcg gcgaccatcg ccctcctcga tctccgtggc ggcggcgtct acctggtcga
     9541 ggcggaccgg cagagagcac ggcttatatg cacccggggt ctcccggatg gttttcccct
     9601 gcagacgtgc atccccgaca tcaccgcacc cccgtatgcg gatgtgctgg tggcgggaga
     9661 gcccttcttc tttgacgatc ccacgcacct ccgctacccg ggggaggaga gggagatgtt
     9721 gcacccttac gccagtatcc cgatcgtagc atccagaggg atcatcgggt cgttgaacgt
     9781 ggtgcctcgc gaccgcaggc ggttcaccga cgccgaccgc tccctccttc tcgccatcgg
     9841 aaaagaggtt gggagttctc ttgagtgcat actgctgatc cggcagcttg aaagcgccga
     9901 gcgggaggcg aacctctacc tcgatatcct cagccacgat atcaggaacg cggagaacat
     9961 atcaagtctc tacatcgata tcctcatcga tatgctcgaa ggcgatgcga aggatcacgc
    10021 acagaagatc cggagcagta tccgaaagag catcgagatc ttacggaacg tctcgacgat
    10081 ccgccggatc catcacgagt cggtcacgct cgcgccggtc gatctcgatg aggtgatccg
    10141 ggacgagatc ggggtattct ccgagatcaa cttcaactat gagggaaccg atcacgcggt
    10201 catggccgat cccctcctcc ccgaggtctt tacgaacctc atcgggaatg ccgtcaagtt
    10261 cggggggtcg gcggtcgaga tcaccatccg ggtggaggag agcggtaacg acgtcctgat
    10321 ctcggtcgag gataccggac cggggatctc cgacgagatg aaagataccg tattcatgcg
    10381 gtttggaaag aacaggatcc agaagagcgg ccagggcctc ggcctctcca tcgtccggat
    10441 gcttgtcgag cgctacggtg gcactatccg gtccgacgac cgggtttcag ggtgccccgg
    10501 atgcggcgcg gcgttccggt tcactctgaa gagagcctga aaattgggtg gggcctcaca
    10561 ggagcgacca gataaggagc cctgcagacg cggccatgag caccgcaaac acggccgggt
    10621 tccaggcgag gatcttgcgc ccatcgagga cgctgatcgg gagcatgttg aacgtcgcga
    10681 tcatggcgtt gacccggaaa ccgacgaggc ccacgatacc gatgagaccg ccgccgaaga
    10741 gcatgaggag cgcaaacggt atgcagagga ggaggttcgt gagtggtcct gctgcagata
    10801 tccgcccgtt ctcggcccgt gtcgcgttcc cgtaaatata cgtcgctccc ggcgccgcaa
    10861 agacgacccc gacgagcgcc gccatcacca cggcgacgag gagcatctgg ttatccttct
    10921 tgaactccgc ccagtagccg tagcgcatcg cagtgaactt gtgcgccagc tcgtggagaa
    10981 tgaaggcgac ccctaccgtg aggagggaca tcacgaagaa gaagatgagc ccggtgacgt
    11041 tcacccctcc ccggatgtag atcagggtga aggcgattga gatggcgagc cacgcgatca
    11101 ggagatcccg gcgctcgcgc agcggtattc gttccagcat ttggtacagg gtttgtcccg
    11161 gcacgataat agccttcgcc tcttccggga gataggcacg gacctggaag gttatttccc
    11221 ggtcgcggcc aaccatggag tatgtccctt gatgccgtca ggagcgagat ccgtgggatc
    11281 gacgagaaga tcatagatct gattgcagag cggcagagac ttgcggcgag gatcgcccgc
    11341 ttcaaacagg aggagggcct ccccatccgc gatggaccgc agcggaaggc ggtcctcgac
    11401 cgggtcttcg ctcacgccgc cgagagccgg atcgacccgg tcgccgtacg gaatatcttc
    11461 gagatcctga tcgatatgaa cgaagagcgg cagcgggagt gcagcgggga tgggaacctg
    11521 ccgtgaaaaa aaagattaga gggtgatcat cgtcccgacg ccctcgtcgg tgaagagttc
    11581 gaggagcagg ttgtgctccc ggttgccgtt cacgacgtga gcgctcgtaa caccgctcct
    11641 gaccgccctc atgcacccgt cgagcttcgg gatcatcccc ccgctgatga tcccttcggc
    11701 gatcatggta tccgcctcag acagcgtaag acggtggtaa gtcatcgtcc ggtcggcgtt
    11761 catcacgccg tcgacatccg ttaagttgat caacttgaac gcctgcaggg caatcgcgat
    11821 ctcggccgcc gccgtatcgg cgttgatgtt caggctcccg ccctgccggt cgatcgctat
    11881 cggggccacg accgggatat agttctgggc aaggaggcag tggagcacct cggggtcgac
    11941 ctcctcgatc tcgccgaccc ggccaaggtc cacctcttcc tcgacctccc ccaccctcac
    12001 ccgctggggc tccatcttcc gggcgatgag gaggttgccg tcgttgccgg atatcccgac
    12061 cgcccgggtg ccgcagttcg cgatgagaga gacgatcccg tcgttgatct tgcccacgag
    12121 gaccatctgg gcgatctcga gcgtctcggc atcggtgatc cggaggcccc cgacgaacct
    12181 gggttctttc cccatcgcct gcatctttgc ggtgatctcg gggccgccgc cgtggaccag
    12241 gacgactttc atccccacgt agcgcagaag gacggcgtcg cggatcaccg tctccaggat
    12301 attctggtcg accatcgcgt ggccgccgag tttgatcacg atcgtcctgc cgtaaaactt
    12361 ctggatgtag gggagtgctt ccatcagcac gtcttcgcgt ttcatgtcgt atacttcccg
    12421 ttgatctcta cgtacttctc ggtgaggtcg cacccccatg ccgtcgccat tccgtcgccg
    12481 gccgcaaggt cgaggtcgaa cgcgacggta tcgccgtgca tggccttctt cgccgccccg
    12541 aggtcggcga cgatctcgcc gcggacgacg agcggcaccc cgccgacgga gagggagacc
    12601 gcgtaggggt cgaactctgc gccggctctc cccgccgccg cgaccacccg gccccagttc
    12661 gggtcttcgc cgtatatggc ggtcttgacg agcggagatg cgacgatcgt ccgcgcaacg
    12721 ccggcagcct cgtcctcgtc ggcggcgccg cggaccgtca cctcgaggag cttcgtcgcc
    12781 ccctcgccgt cgcgggcgat ctggatggca aggctgcggc agacggcctc gattgctctt
    12841 gcaagttcgt cgcgatccac ccggcccgcg gtaccggtcg cggtgcagag agcgacgtcg
    12901 ttcgtgctcg tatcgccgtc gaccacgacc cggttgaagg accgccgggt agcctcgcgg
    12961 aggatatccc ggagggccgg tgcctcgacc tccgcatccg tgtagatgaa cgcaagcatc
    13021 gtccccatgt tcggggcgat catcccgctg cccttggtga tcccgccgac ggcaaacgac
    13081 tccgtccgga cgagggcgtg cttcgggaac gtatcggtcg tcatgatcgc ccgggccgcc
    13141 gcgtcttcgg catccccgtt ccgggcgagg agcggcgcca cccgcctgca ctggtcccgg
    13201 atgaggggga ggttgaggta gcgcccgatc actccggtgc tcgcgacacc gaccgactcc
    13261 ggtgcaagct tgagggtttc tgccgcgata tcgcacatct caagtgcgtc gcggcatccc
    13321 cgctcgccgg tgtaagcgtt tgcgcaccca ctgttcacgg caacggcatc gagggttcct
    13381 ctctccatcc gcttcatcat cacctcgatc ggggccgccc gcaccttgtt cgatgtgaag
    13441 acaccggccg ccgtcccgcc tgcccggatc agagcaagcc cgaactttcc ttccttgatc
    13501 cccgaggcgc tgacgccttt caccgcacag atactcttca tgccccttcc ccctccccga
    13561 tgccgcggac gatgtcggcc cgggtgacga taccgatcag ctttccgttg cggataaccg
    13621 ggaggcgtgc gatcctctcg cggagcatca gggatgctgc atcggcgata tccgactctt
    13681 catcaatcgt gacggccggg ctgctcatca cccggcgcac ctccctatcg ccgatatcgg
    13741 tgagcgcccg cttcgtcttc tcccagttga tgaactcgcg gaccggaacc tcgatcacct
    13801 cgagcggcga cgggagccag aggtcgcttg agaggctgcc ggtatcgagg agcgatatga
    13861 tatcggtctc ggtgacgatc cctgccaccc ggtcgccgtc catgacggga aggccgccga
    13921 tgtgatactt ccggagcaat cccgccgcct cgcggatcgg tgagtcgacc cggacggtca
    13981 cggggtcggg ggtcatgatg tctttgacct gcaccatcgc gaccacctca gggggacatc
    14041 ccggcgaagc acaggccggc accttccgca aacccgcaca tcaggttcat gttctggatg
    14101 gcctggccgc ttgcgccctt gaccaggttg tcgattgtag agaccgcgac gacccggtcg
    14161 ccttcgctct ccaccgccac gtcgcagaag ttcgtcccgc gcacggcggc aagggtgggc
    14221 ttctggtagc ggacgaagaa ctctctctcg tagaacttcc ggtagagcgt ctcgacctct
    14281 tttgactcca tcggctcgtt gaggaggatg tgggccgtcg tcagtatgcc ccggttcacc
    14341 gggaggaggt gcggcgtgaa gtagcaccgg gcggttgaac cgagccgggc ggcctcctgc
    14401 ttcatctccg cgaggtggcg gtggctcgtc cacttgtagg cgctgaagtt gtcgccgacg
    14461 ttcgggtagt gggtggtcgc ggagggtgtg ttcccggccc cggtgacgcc ggtcttggag
    14521 tcgtatatga tggtgtgggc aaggtgcgcg agcggtgccg ccgcaagcgt cgccccggtc
    14581 gggaagcatc ccgggtttgc gacgaacgag gcgcccctca cctcgtcgcg gtggagttcg
    14641 gtgaggccgt agggcgcctc aaagtaggcc tcgtgggtca cgccgtaggt cttctcgtag
    14701 atctcctttg agagccggta gtcggccgag aggtcgacgg tcctgatccc ccgttctaca
    14761 agcgttccgg caaccttcat cgccgcggtg tgcggtacgg cgaggaagac aaagtcggcg
    14821 tcgatatcgc cggccccgat gttccggaag gcgagatcgg tcagtccccg aaggtggggg
    14881 tggaccgagg cgacgggggt gccgtccagt tttcgggatg tcgcggcgac gacctctgcg
    14941 gacgggtgat tcagcaggag ccgcatcaac tcgccgccgg tgtatccgga tgcaccgatg
    15001 atcgcaattt ccataggaga gaggtgtctt ttgcagaatc ttaaggcttt gtggtgcatc
    15061 gctcgtagag acgggccgcc accgactcga tctcgctccg gcactcgagt ccttcgagga
    15121 gatctcccgg cagcgcggtt ttcccgtaga ccgcgcctat gtaggcgccg cagatgagcg
    15181 cgatggtgtc ggtgtttccc ccgacgtgcg cggcggtatt gaggagagct gaggcatctc
    15241 tgatgcgatg gataaggaag aacgcgaggg ggacggtctg gtagacggtc acgtcgttac
    15301 ctatcaccga taacgcgctt tcaaggctga tcccctcgtt tgcgagccgg acggcgtcgc
    15361 ggatcttgtt gccgagggtg acgtcctcaa ggcccgcgtg tttcccggca agcgtgagcg
    15421 cgtcggatcg gccgcggacg gcgtagtgga cgagcatccc gacggtgacg gcccccgcgt
    15481 gcgccgccgg gtgggtatgg gtaacgctgc aggcctggac cacccgttcg gtcacgtcga
    15541 ccggttctgc gtaggcgagc gcgaacggga tggcgatgct gatgcagccg gcggtgttgg
    15601 aggcgacgcc gaccgatttt tcgcccgaga ggaggagatg ccggcacgcg gcgtccacgg
    15661 cgccgtcggg gaaccggagc tcttcgttca tgtacatccg ggcaagggcg gccgcgtagg
    15721 cactctccga gtaggtgccg tcggcgagca tcccggcgac gaggagcatc atctgggtgt
    15781 cgtcggtgaa ctgcccgggt ttcagcccgg cgttcgggtg cccccgccac gcccggcggt
    15841 acccctcgtg gaggcgctgg aagtcgggcg gggtgctttc ccgcgccatg ccgagggcgt
    15901 cgccgatggc ggccccgagg agacagccct tgaactgatc gagcatttag tagtatctct
    15961 cgtacagttg ctcttaaggg ttgttgttct gcgcgcgggg gagaacggcg gccgtccggt
    16021 caactcccgg gtgcctcaag ccttcccgcg ggacttccgg acgaggacat accctgccag
    16081 gagcacgatc acggccccga gagcgcacat gagaagcacg gggttataga agaagctgaa
    16141 cacatcgtta gatccctgat atgccgtcgt atctgcaggt tccgcgagcg cggccgacgg
    16201 tgccgcgccg tctgttgggt tggcctgcac gtcttcaccc ggatccgtgg ctgcatgtgc
    16261 tatgccgctg ccgatcagga tgaagagcag cagggcgaca cagatcaacc gcttctggcc
    16321 gatacagttt gtaaattgct tttctctcat cgatcatcat ctgccggtgc gccccgggac
    16381 accggtcccg gtgctcatgg tggagacgtc gacgctagcc tataaacctt ttctgtgccg
    16441 cgcgtctgca cgtcgtgtct tcgtgaccga tttcgggccg aaaatcgccg cagtgggccg
    16501 gaaaccggag ccgcgcaggg gagccttcac tccggtaacc acccggcaac cggaaccctg
    16561 atacgggact gtccaatatt ccggacatat gtctaaaata tcggacattc gggagaacca
    16621 cctcatggtt ctctccctct tcacccgggg ctacgatcgc aaactttaca tcggggaggc
    16681 cgcgaggctc cttcccatca gccgcggaac agctcaggcg gtccttgaat accttgaggc
    16741 aaagcaggtt ctccgctccg ccaccggggg cagggcgcgg ttgttctccc tgcggcgggg
    16801 cgatcatgcg cgggacttct acacccttgc agagatctac ctggggatct gcttccggga
    16861 ggaggagccc atcctcgccg gagttctcag ccgaatcgcc cccttatcga gggatgcggt
    16921 gcggtcttcg ggagtcacgc agaagggact gcggacgagg agtccggcct cgacctcttc
    16981 gttgccggtg agtgccgggt ggaggaaatt gagaggatcg gaaagaccta cggctttccg
    17041 attcacctca cgatctaccc gcgggaggtc ttcgaccggg atctccatac ggaccccttt
    17101 ctccgggaag tgctccggaa ccacgtgctg atcaaaggcg ccgaacactt cttcgagaag
    17161 gcggtgaggt gacggatcgg ctcgtatggt gcaaccggat caaaaacggc atctccctct
    17221 ccgcaccgaa cgagaccctg gcgtcggcat acttgaagaa ggccgaagag gcccttgaga
    17281 cgatgcagac gattacggcc cgcgactgaa agataacgac cgcgtattac gcgacgtact
    17341 tctccctcga tgcggttctc acgtggatcg ggatacggtg cgagaaccat ccctgcacca
    17401 atttgtccat ggaacgactt ttgtcagatt atttcactcc tgcggagacg gcactcgtgg
    17461 aaaaagcaag aggagcacgg atcgatgcat ggtactatgt ctcacgggaa gttccggatc
    17521 ccttctgcga ggaacttatc tgggcggctc cgcgattcct ggtgaagtgt ggaggcatcg
    17581 ccgacggaat gaatgagaag accatcgaga ccctgagggg ggcgctacga aaagaggagt
    17641 gagagggcaa gctggctcat ggtcgctccg ggcatcactt gccgatcgat gcggcaacct
    17701 tcgttatctc acctgcaggg agccacccag tcatgcagaa ggaggcatcg ttccactccc
    17761 accggagcag ccagagggag tggaacgatg ccctcctctt ctccggtgaa ccggctaccc
    17821 gggagtcggc cgatacccct gtaccggcag ccctccggtc atcaggttct tcccgtcgct
    17881 atagcgtcta tcaggctctt tacaaggctt gtaatccgat tttcggtaat atccggagag
    17941 tccctggccc cggccggcgg cacgcagcaa tcgaagatgg gggaccggag tctcggggtg
    18001 atgtcccgct cgtcatagat gacgtgcaca accctctgct ctccgtcgat ctcgacggtc
    18061 ccggcctggc cgatgacgag caatgtcggg ccgcaccgga tcgtcccgag gatcgcgtcg
    18121 ccgccgaccg taaacgaggc ggaactcccc ggatccccta cgaccgtccc ctcaaagcgc
    18181 catatatgag gaagagcgac ttcaaacgtg cccgactcat tcttgacaaa ggcccgggcg
    18241 ccttcggcga cggggcccgg caccggttcg aggtcgagta cgaactcccg gtcggggagg
    18301 cggagcgtca ccgggctccc cgagcccgcg ccagcgacga acgccgccgg gtcggcggtc
    18361 acgaggtcat actttcggag gggcgggagg ttcgtgaggt tcacctcgcc ggccgatggg
    18421 accgggctga agtaggcggg atcggccacg gtcacgtgcg ccgtagggcc tgacggcagg
    18481 cccgatgccg ccacgtatcc ggcatccggg aacgtcacgt tctccccgag cagaaccagg
    18541 ccgggatgta tctcggccgg ttccgggccc ggctgctccg gttcttttgt aactatcggc
    18601 ttgttgtacg tcgacatgcc ccggggtgtg acccgttcgt caaaggtatc aagccagttt
    18661 gtgctctccc cgccggcaga gatcaccggg acgaagaacg ccccgatgag cagtgatgcc
    18721 agcagcaggc cgaaaagccg cactttgatg ccgttcatca tatcccgaat cacccccctg
    18781 catcagcaaa gacatacccg atatacggtt caggaacgcc gtctcggaac gctgttccgg
    18841 aaaacgcata caccggccgg acctgatcct gtacgacacc cctcggttcc atccagtaac
    18901 cgatcgagac attctcgatc gtgatccgac cgtactcccc tcccacacgg gggcggatgg
    18961 tccttgctgc aaggagatcc tcatatgcct ccccggggga ccggaggctg atattcccgt
    19021 ccggcgtcac ctcacgccag gtcttcacga gcccggcgac cttgccgccg tcccccagga
    19081 tcaccgtaaa ttcgtcgccg tagaccggca gcccgtcgag cggccggccg aaccgaaccg
    19141 ttttcgtcac atcgcaggac acctccggct cgctctcgcc ggccttccag acctcctgct
    19201 tctggcccac cccaatcttc acgacccggg cacccggcga ctgcatgccg gtcttctcaa
    19261 ggaacgcaag cgcaatcctc tctgcctccg cgtccgtcgg gaggtccggc tgccggatcg
    19321 cttcggtcgg cagttccagg tcggggatgt gataggccac ggcgccggaa tgtgcataca
    19381 ccgatacctg ctcctccggg tccttcgagg catcgatgag gaggaactcc ccggtctttt
    19441 tgttcatcgt cccgggttcc cctgcaagcc cgaaccgctc cgcaacctcc cggacctgct
    19501 cttccgtgac ggtgacgggg actgtccggt agagcgtgta cgagtcccgc atctccggga
    19561 actcggcagt gagggagaac atagtttcac ctgcacccgc tctggcagga agagggattt
    19621 ccggagcctg gcccggacca agaacccata cgcacccggc tatgatcgcg agagcgagac
    19681 agacgagtgc aacaattttt aatcgcatgt caaaacctct gattggatgc cgcggtagat
    19741 tcagcagccc gaggcgggag gggcatgcct tcgcacacag aatgcataga ccctgttgcc
    19801 gcttcccgtg ccgagcaggg ggcgagcggc cttgcaccat gaaacgaata actcctgagc
    19861 ataaggttct ccagggtagc agtgtggtca gggagaatgg ccggcccgac caccccatag
    19921 tagacaatga agtaatcgtc gtgttttggg gacagcggct tctcaacaac ggcgagataa
    19981 cctgaagacg tcttgttatc gaattttgtt acggcaacgg gactaaaaac cggttctccc
    20041 cgtgataatt caggttctct ggagaggaac atctcagcag aagagacatt ccccgatgct
    20101 cggaggtgga tgcagaggac cgtctcagcc tgtaagaggt ctgtcttatc gtcgaaatac
    20161 cagttgacga ccagataccg atcacccgac atggtgtcga catacattgc atggctgcag
    20221 tgccgtgaga gaaccggaaa caaggatgga cacccgtctt cgatatcgcc gagcaggaca
    20281 acattcccat caatcgttct atttgcggcg acagagccgt tctcggcata ttcgtaccgg
    20341 ggctgcggga tataccattt ctgcagggct ttagggggag gatacccggg accaatggcg
    20401 aggagatcaa cgagcaggag gatgccgaaa gcaccgatag tgatcaggag gagaatgaat
    20461 gcgattttgc tggttctgtc ggttttcaga gatttcatcc ggatcttcca ccataaagaa
    20521 ggggtcgggg cctcacccgg gacgcatccc ggatgccagc gggctcgc
//
LOCUS       DAOSII010000019        19991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_2943_length_19991_cov_53.912972, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000019 DAOSII010000000
VERSION     DAOSII010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 19991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19991
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2943_length_19991_cov_53.912972"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="PLV90_02700"
     CDS             complement(<1..1115)
                     /locus_tag="PLV90_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOF96055.1"
                     /translation="MVYRFAGRMGRVPDSFLDELFRVSAVPGVISFAGGLPGSAYIDV
                     EGIRDAARDVFTDEGRTALQYTTTDGYLPLREFIADRYRARLGLPATPEEIQIVNGSQ
                     QCLDLVAKIFLDPGDAVGMERPGYLGAIEAFSLYEPEFCSVPLTDDGPDLDRFASLVR
                     EHAPKFFYGIPNSQNPSGMTYSDDTRRGVADILEGTDTVFYEDDAFGELFFDGRPRPP
                     VKRYLPDRTVMSGSFSKIVAPGMRIGWIYAPAPILREFNVAKQAADLHSNFLCQVILH
                     RYLATHDLDAHVARVSAVYGRHCRLICDLLDDLMPPGTTHTTPEGGMFMMVTLPEGVS
                     SMEVFREGVREGVAVLPGVPFYVGGGGEDTIRLNFSAA"
     gene            complement(1153..2892)
                     /locus_tag="PLV90_02705"
     CDS             complement(1153..2892)
                     /locus_tag="PLV90_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2070 family protein"
                     /protein_id="HOF96056.1"
                     /translation="MESSPDVRFERLTRYLFSAPSWPRSLLLIILLGVVIDIAAYRVG
                     NGFFLVGTLGFALPALVAFLLTVPLVEVFGRQITYNRSGLLALACTVLMVILSLSPVL
                     VFGRELFSALYAGVLGLATGIRLLVLVAVADYRITRMAAPALLQGVTGIAVGAWIFTP
                     GFVPYAILLQVVFGLVFVLLIGLIERPLKRAFQVSGLNFLNAFIAHLTDGSKSMEAFF
                     RDIGEEVCVQQASLFFSRDSGEDVLFTVPNVHPGPMGDVGGGNLPRVLHDTFAAETLV
                     AHGCATHDFNLVSESEIETIARAIEASGEGISFADTASRPVRVASGSVSILCQRFGDA
                     LLMVSTRSPERTEDLDYSIGMVIMAEGRCFFSEVAFVDAHNCMTSVGSPVLPATRIAM
                     EYIDAAREGFSAARDLPAEPLAVGVSHVRVPFTREQGFGSLGVQALVTDAGGKRAAYV
                     LVDGNNMAPGVRERLLEVVLGYADEAEIMTTDTHTVNTISGKNPVGYVVPVDAIAPYI
                     EQAVREAVADIAPARVGAVTASCEGVVVFGSQRVSQLASTVNAMLAFIGPISLIILLL
                     AFLLSVFAYLLLQ"
     gene            complement(2958..3848)
                     /locus_tag="PLV90_02710"
     CDS             complement(2958..3848)
                     /locus_tag="PLV90_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOF96057.1"
                     /translation="MIAVVGHTAIDHLFSVPGLPGRHNSTYIVDHQVYFGGGAANIAA
                     GIATLGERCRLVSPVGGDFPGSDYDRWMRDLGIVQDFTLVKEARTATAYVFTDEAGDQ
                     ETFFEWGASAAFSRAEAPALDFVHMATADPDFNVRVAEKSRFASFDPGQDLLRYTPDQ
                     LEIILAKIDILFSNNHEMDRMCDMLGLERPALIASVPIVVTTRGAEGSVLCMDGEEHH
                     VPAVRVNAVDPTGAGDGYRAGFLTAYRRGYAPIDCCRAGAVVSSFVVERPGTQTNLPD
                     WERMLVRYRKVFGEPGEISP"
     gene            complement(3845..4183)
                     /locus_tag="PLV90_02715"
     CDS             complement(3845..4183)
                     /locus_tag="PLV90_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF555 domain-containing protein"
                     /protein_id="HOF96058.1"
                     /translation="MPDYLVTLESAWIIKDVKSMDDAVSIAISEAGKRLNPSAKFVDI
                     EAGMIACPYCEGELNSALVVANTALVGLVLQMKVFRAESGEHASRIAKSVVGKALRDV
                     PLKVQEVQEV"
     gene            complement(4204..4731)
                     /locus_tag="PLV90_02720"
     CDS             complement(4204..4731)
                     /locus_tag="PLV90_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOF96059.1"
                     /translation="MTSSDLLRLLKARSSVRDYTGEPLDPEDIDYILTCASTAPSAGN
                     REAWDVVVVTDEDLRIGLAVAALEQMHIREAPAIFVVCANYVRSMSQYGERGILYALE
                     DATIACTYMMLAAHARGLHSCWTGAFDENDVRETLGLPEHIRPLALLAVGKGIPPLRP
                     VERMPVEEHVHRETW"
     gene            complement(4718..5074)
                     /locus_tag="PLV90_02725"
     CDS             complement(4718..5074)
                     /locus_tag="PLV90_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96060.1"
                     /translation="MDLKTAVRTYQFAERAKSELIVGSQLTTALIQFPLQEKPGGKRM
                     LLMVLQSIRSELEFAYGDTEQREFRKAIDHLNEGISLTESMELGAASERMARAVSAVT
                     TAAQTAWDTLKEHDLL"
     gene            complement(5131..5439)
                     /locus_tag="PLV90_02730"
     CDS             complement(5131..5439)
                     /locus_tag="PLV90_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5350 domain-containing protein"
                     /protein_id="HOF96061.1"
                     /translation="MGKTGSVKWAQVKGVKGQIRLVPASEGEVKKPGPNQRFKPLADI
                     RKRASREGQDQRRGGRGGRGGRGGRGARGGGTPTMDVRVRRSIRRAKVSALGTKQKSR
                     "
     gene            complement(5527..7386)
                     /gene="gatE"
                     /locus_tag="PLV90_02735"
     CDS             complement(5527..7386)
                     /gene="gatE"
                     /locus_tag="PLV90_02735"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HOF96062.1"
                     /translation="MDYKDLGLKAGIEIHQQLDTAEKLFCRCPTVLRDVADRTGEFYR
                     YLRATESELGEIDRAAEEEMKLVRKFCYYTYDTVCLVDHDEEPPTPMNPEALDICLTI
                     AKMLGMTPVEQIHTMRKLVVDGSNTSGFQRTALVALSGALPDGCRIETICLEEEAAQR
                     VEGETFSLDRLGIPLVEITTAPCMRTPEAVQEVAAYIGMVLRSTGRVKRGLGTIRQDI
                     NVSIAGGARVEIKGVQELDLIAEVVRREVDRQTNLLAIRDALRDRGARVDRTVVDVTA
                     LFAGTKSAVLKKAKFILAIRLCGFAGLVGREIQPGRRLGSEMSDYAKKCGVGGIFHTD
                     ELPAYGVTAEEVARLREFVGAGDDDCVAIVAASRERAGCAAEQVMIRADMALAGVPEE
                     TRKMLEEGSSAYMRPLPGAARMYPETDVFPIALSGALWEGIRVPELLTDRAERFVREF
                     GLDGALARQVAFSERLPLFERAVAAGVRPTLAARTLLATCRELARGGVAIDRVSEDDI
                     LALLSAVEAGRAAKEAIPDLLAELARTADESAGTPRERVDAAIAKMAPAVSQADVESI
                     VRRIVAEREAFAREKGMGALGPLMGVVMQELRGSVDGKVVSETLRRELKRLLS"
     gene            complement(7387..8619)
                     /gene="gatD"
                     /locus_tag="PLV90_02740"
     CDS             complement(7387..8619)
                     /gene="gatD"
                     /locus_tag="PLV90_02740"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HOF96063.1"
                     /translation="MTESLEAGDRVRYANGGTALTGIYIAERDGMAVIKLDSGYNIGA
                     SPEKLERVGRAAPQPPAGAGIVIQDPALPELSIISTGGTIASRIDYRTGAVTSQFSAN
                     DILRSIPELGDIARYRDRQVASILSENMRPAIWQALARAIYDEIRHGTSGVIVTHGTD
                     TMASSAAAVRFMLRTPVPVVFVGSQRSADRPSSDNAMNTLCSAAVAAGDLGEVAVVMH
                     ATTNDDRCAVHRATRVRKMHTSRRDAFQSMGMAPLGSVDYPSLAVTLSDEAVRRGAEE
                     PGLHDTLEERCALLQFYPGMPPGILDAFEGYAGLVIVGTGLGHVATDWIPKLRDRIEG
                     GTTVVMTSQCLHGRVCDRVYNTGRDLLRAGVIEGEDMLPEVALVKLMWVLGNEPDPER
                     AGVLMQTDLAGEIRRRSI"
     gene            complement(8616..10094)
                     /gene="argH"
                     /locus_tag="PLV90_02745"
     CDS             complement(8616..10094)
                     /gene="argH"
                     /locus_tag="PLV90_02745"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOF96064.1"
                     /translation="MRSDQIRQGRLSGERSADLEHFLASMAADRWIAEADLLVDMAHL
                     LGLSRQGIIDEAPARALMAALLDLHDHGLPEEAFDEHYEDIHAGKEAYLIGRAGEDFG
                     GRLHMGRSRNDEVATCIRIRLKQELIALVQSLAGLRRTLLDVAAAHTETVMPGFTHLQ
                     HAQPTTLAHHLLAYEQAFSRDTARLREAYARVDVSPLGSAAFASTGFPLDRDYTARLL
                     GFSRPAPNSMDAVAARDFAIEVLGDIAALMTTASRFCEELVIWSSAFVGFVHLDDAYC
                     SSSSIMPQKKNPDVAEIMRARAGSAAGSLIAAITIVKGLPMSYNRDLQELTPHLWRGV
                     EAARQSIPLLSGMIATAAFNTERMAAEAGRGFSTATELADVLVREYGLPFRTAHRIVG
                     RAVRHGSLDIATLEGAAREAAGLSVVDLGLTEERIALALDPRHAVAVRTIVGGPAPAA
                     VAVQLTEQEELLARDRAWAEETDTALSRAFEDLISESRRLIL"
     gene            10581..11567
                     /locus_tag="PLV90_02750"
     CDS             10581..11567
                     /locus_tag="PLV90_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOF96065.1"
                     /translation="MRSITATLLVVAMVAAFACIAGCTGTGADDMTHLRIGYQPSTHQ
                     IAHFTAMEKGWWQEDLAPLGITKVTDNQFGTGPPEMQAMLAGELDIAYVGAAPVVSAL
                     ASGLDAKIIAAVQTQGSDLMLRNDIAYESPQDLVGKEIATYHPGSIQDTLLRTWLEKN
                     GVDPAKVIIKPMDPGAATTAIAAGKVDGVFLPDPYPAIIIAEGTGRTIVQSGEMMKDH
                     ACCVLVATGSLIREHPDIVEQIVRTHIKATEYNFEHQDEAAELYSAKTGQNLDTVKAS
                     FKIWDGTWTADPRIIIPSTIEYTNLQYELGYIKKPLTQDDLFDLSFYEKARA"
     gene            11703..12479
                     /locus_tag="PLV90_02755"
     CDS             11703..12479
                     /locus_tag="PLV90_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF96066.1"
                     /translation="MNRQTQKRLLGITALVTVAVIWQIIAEYVVGRSFILPSVTDVIS
                     AFVDLIGSGTLLTDITVSLEHFGIGLLAAFFLGVPIGILMGWFRTADFLLDPIIEVFR
                     PIPPLAWIPFSIIWFGLTTQAAGFMIFVGAFFPILTATYTAFRSIPKVFVEAGKVLGC
                     NTSFELIRHVALPAAIPAIASGIRIAMGVGWMCLVGAEMFGVSNYGLGYQLWHNYYLH
                     QMPNVVVYMLILGFTGLIIDRIFRNYVDGRLLRWHAGEVA"
     gene            12480..13241
                     /locus_tag="PLV90_02760"
     CDS             12480..13241
                     /locus_tag="PLV90_02760"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF96067.1"
                     /translation="MGDVSIRDLGKAFPKEDGTVTQALKGIDLEIRDEEFICLVGPSG
                     CGKTTLLRIIAGLETATTGEVTVDGRAVTGPDPKRGMVFQEYSLFPWRRVIDNVAFGL
                     EMKGVKKEERHKTADYYIDMVGLSQFRDAYPYELSGGMRQRVAIARALANDPDVLLMD
                     EPFGALDAQTRNRMQKELLSLRDKTKQTIVFVTHSVDEAVYLADRIIVLSPRPATVRE
                     DIAIPWPRPRDRTSAGFAEVRRRVLDMINETGSVQ"
     gene            13303..13815
                     /locus_tag="PLV90_02765"
     CDS             13303..13815
                     /locus_tag="PLV90_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOF96068.1"
                     /translation="MVRKPAKMYRNLAKKAYTRREYMGGVPGSKIVQFDMGNLTQDFP
                     VELSIVVEETCQIRHTALEAARIGINRQLLKEVGRANYHLKIRTFPHHVLRENKQATG
                     AGADRVSEGMRLAFGKAVGTAARVQSGQKVFSVWTTPQYAEKAKVSLKRGIYKIPTPA
                     RIVEEQTMEA"
     gene            13921..14757
                     /locus_tag="PLV90_02770"
     CDS             13921..14757
                     /locus_tag="PLV90_02770"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOF96069.1"
                     /translation="MTHPADPALLRALATATEKALLEAEIRLPTDVLAALRRAAAAEK
                     NEVARQEFALILENIALAGERQVPICQDTGVPVVYLTLPPDVPFTPDLFAGIREGVRR
                     ATTAIPLRPNVVDPLTRENTGDNTGAGMPAVHVVFGDRLTVTVLPKGAGSENASRIGM
                     LLPSQAADIPRFVAETMLIAGGKPCPPVFLGVGIGGTFDGAAALAKEALLLPVDTMDD
                     YEQEICDAVNALGIGPMGLGGETTALAVKVKRRDCHTASLPVAVNVQCWACRRATVEV
                     ER"
     gene            14724..15296
                     /locus_tag="PLV90_02775"
     CDS             14724..15296
                     /locus_tag="PLV90_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOF96070.1"
                     /translation="MPPGYRGGGAVNLTTPLGDEVLDLRAGDRVTLTGTIYTARDEAH
                     LRMMEEGIPFDPVGAAVYHCGPVVRDGRLVVAGPTTSARMNALTGFLIDAGVRALIGK
                     GGMGPEVVEQLRGRAVYLAFTGGCAALAADRMSLSGVYFEDLGMAEAVWIIEADHLPL
                     TVGIDARGGDLFSAVREKAKTQFHQRFNIR"
     gene            15308..15580
                     /locus_tag="PLV90_02780"
     CDS             15308..15580
                     /locus_tag="PLV90_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOF96071.1"
                     /translation="MKLVIDESRCKGCNLCVLVCPYGIFREGTELNSRGIVSPVLDRP
                     ERCTNCRLQALYGRMLCGICHMICPDQAISWVEEKPFEPHRVEVEF"
     gene            15577..16677
                     /locus_tag="PLV90_02785"
     CDS             15577..16677
                     /locus_tag="PLV90_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOF96072.1"
                     /translation="MSRVEFMQGNAACAEGALAAGCRFFGGYPITPSTEIAEAMARRL
                     PKAGGVFISMEDELASIAAVIGAAWTGVRAMTATSGPGFSLMMENIGYAAMTETPCVI
                     VNVQRGGPSTGQPTRAAQGDMLQCRFGSHGDYSIIALTPNSVQETFDLTAKAFDLADR
                     FRVPTFLMTDEIVGHMRERVTIPDAVDITRSSPLAEGSLPFEAGDDGIPGFAPFGSGR
                     AVHVTGLTHDERGYPDTTDPAAHDRLVRRLVAKVESARRAIADYEVTNPDAGTVFVTY
                     GPPSRTVEQVMRDYPDDAVGHLRLRVVWPFPEFALHEFPNAETYIVPELNMGQVVREV
                     ERHVDRPVVPLGKIGGELHTPAELVRALEAHR"
     gene            16674..17462
                     /locus_tag="PLV90_02790"
     CDS             16674..17462
                     /locus_tag="PLV90_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOF96073.1"
                     /translation="MIAPDWLREDRLPHIYCTGCGNGTVINCTLAAVEEMGWKRDETV
                     FVSGIGCSSRAPGYILTDSLHTTHGRALAFATGVKMARPEFNVVVFTGDGDLAAIGGN
                     HFIHACRRNVDMTVVCMNNHIYGMTGGQGSPTTPPGAISTTTPYGMSEPAFDLAELAV
                     AAGANYVARWTSYHVKELTKAITAGMRTPGFAFIEARTQCPTNYGRHNKLRTVPAMIE
                     HIRSHAMLIEKRDRLVREGRPVPEDIFTIGELVRRNRPAMGVRR"
     gene            17459..17998
                     /locus_tag="PLV90_02795"
     CDS             17459..17998
                     /locus_tag="PLV90_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOF96074.1"
                     /translation="MRHEVRFSGYGGQGIILSAVILGRAAALYDGKHAVQTQVYGPEA
                     RGGASMGQVVIDDRPILYPEVADPDIYAIMSQQGFEKYGVRARPDAVMLLDTDLVRSR
                     PGCRYYEIPATAEAKTVLGREIVANIVMIGALVTSTGIVSREAIERAVLDSVPKGTEN
                     LNMKALKRGFELGERACNL"
     gene            17995..19068
                     /locus_tag="PLV90_02800"
     CDS             17995..19068
                     /locus_tag="PLV90_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOF96075.1"
                     /translation="MKLLEYEAKQIFSEYGIPVPKGALIRAPEEAAAHLPETGGAVVL
                     KAQVDVGGRGKAGGVLFADAKTAVDTARELFAREIKGIPVREVLIEERLAIEHEYYVS
                     IAVDRSSRQPAVLFADAGGVEIESAASAVRKVIVSPLLRDIPPFLMRELLGGAPKVLA
                     PVINRLYRVFQEKDAMLAEVNPLVTTPQGVYAADAKLIVDDNALARQGIAVNRDLSER
                     EREAEKHGFSYVELDGTIGVIGNGAGLTMSTLDLIEFYNGRAANFLDVGGGADQERVM
                     HAVRLVASMPDVSVIVVNLLGGITRCDEVAKGIIAAGVAPTVIVRMAGTNEAEGRQLL
                     GEHGYRMLESMDAAVRAAMEVAP"
     gene            19065..19931
                     /gene="sucD"
                     /locus_tag="PLV90_02805"
     CDS             19065..19931
                     /gene="sucD"
                     /locus_tag="PLV90_02805"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOF96076.1"
                     /translation="MIYGDKSLGVIVQNITGRQGMFHTELMNAYARDLGGRGVVAGVA
                     PGKGGREVHGVPVYNTVKEALREHDATRSVVFVPGGAAGDSIMEAAHAGITLAVVITE
                     HIPVHDAMKAIAYARLHDCTVIGPNCPGLLSPGECKLGIMPAHLATRGNVGVVSRSGT
                     LTYEVVDELTRAGIGQSTVVGIGGDPVIGQTFVDVLARFEDDPLTKAVVVIGEVGGNL
                     EEEGVRSTDLPVVTYIAGVSAPPEKRMGHAGAIIEGGEGDARSKVRRLAALGIPVASR
                     PSEIPGLIREVL"
BASE COUNT         3599 a         6602 c         6575 g         3215 t
ORIGIN      
        1 gccgccgaga agttcaggcg gatcgtatcc tccccgccgc cgcccacgta gaacggaacc
       61 ccggggagga ccgcgacgcc ctccctgacg ccctcgcgga aaacctccat cgacgagacg
      121 ccttccggca gcgtcaccat catgaacatc ccgccctcgg gggtggtgtg ggtcgtcccc
      181 ggcggcataa ggtcgtcgag gaggtcgcag atcaggcggc agtgccgccc gtagaccgca
      241 gagacccggg caacgtgggc gtcgaggtcg tgcgtcgcaa ggtagcggtg gaggatcacc
      301 tggcagagga agttcgagtg gaggtcggcg gcctgctttg cgacgttgaa ctcgcgaagt
      361 atcggagccg gcgcgtatat ccagcctatc cgcatgcccg gggcgacaat ctttgagaac
      421 gaccccgaca tgaccgtccg gtccggaagg tagcgcttca ccggcggccg cggcctcccg
      481 tcaaagaaga gctccccgaa agcgtcgtcc tcgtaaaaga ccgtatccgt cccctcgagg
      541 atatccgcga cgccccgccg cgtatcgtcc gaatacgtca tccccgaagg gttctgcgag
      601 ttcggtatcc cgtagaagaa cttcggggcg tgctcccgga cgagcgacgc aaaccggtcc
      661 aggtccggcc cgtcatcggt gagcggaacc gaacaaaact cgggctcgta gagcgagaaa
      721 gcctcgatgg caccgaggta gccgggccgc tccatgccga cggcatcccc cgggtcaaga
      781 aagatcttgg cgaccaggtc gaggcactgc tgcgaaccgt tcacgatctg gatctcttca
      841 ggcgtcgccg ggagaccgag ccgcgcccgg taccggtcgg cgatgaactc ccggagcggc
      901 aggtagccgt ccgtcgtcgt gtactggagc gccgtccgcc cttcatcggt aaagacatcg
      961 cgggccgcat cccggatccc ctcgacgtcg atataggccg aacccggcag cccccctgca
     1021 aacgatatca cgccggggac cgccgagacc cggaagagct catcgaggaa cgaatccggg
     1081 acccgtccca tccggccggc aaaacggtaa accatacgat catattgggc gcaccggcaa
     1141 aaagatgccg gcttactgca acaggagata cgcaaagacc gagagcagga acgcgagcag
     1201 gagtatgatg agactgatcg ggccgatgaa cgcgagcatg gcgttcacgg tgcttgccag
     1261 ctgcgatacc cgctgcgacc cgaagacgac gaccccctcg cacgaggccg tcaccgcccc
     1321 cacccgggcc ggggcgatat cggcgaccgc ctccctgacc gcctgctcga tatacggggc
     1381 gatcgcatcg accggcacca cgtagccgac cgggttcttc ccgctgatgg tgttcaccgt
     1441 atgcgtatcg gtggtcatga tctcggcctc gtccgcgtag ccgagcacca cctcgagcag
     1501 gcgctcccgg acacccgggg ccatgttgtt cccatccacg aggacgtagg ccgcccgttt
     1561 ccccccggca tcggtcacga gcgcctgcac cccaagcgac ccgaacccct gctcgcgggt
     1621 gaacgggaca cggacgtgcg agacgccgac cgcgagcggt tctgcgggca ggtcacgtgc
     1681 ggcggagaac ccctcgcggg cggcatcgat gtactccatc gcaatccgcg tcgccgggag
     1741 gacgggggag cccacgctgg tcatgcagtt gtgggcatcc acgaacgcga cctccgagaa
     1801 gaagcaccgc ccttcggcca tgatcaccat gccgatcgag tagtcgaggt cctccgtcct
     1861 ctccggcgac cgggtgctca ccatgaggag agcgtccccg aaccgctggc agaggatcga
     1921 gaccgacccc gacgcgaccc ggacagggcg gctcgccgta tcggcaaaag agatcccctc
     1981 gccggacgcc tcgatggccc gggcgatcgt ctctatctcg ctctccgaga ccaggttgaa
     2041 gtcgtgggtg gcgcacccgt gggcgacgag ggtctccgca gcaaacgtat cgtggaggac
     2101 ccgggggaga ttcccgccgc cgacgtcgcc catcggcccg gggtggacgt tcgggaccgt
     2161 gaagagcacg tcctccccac tatcgcggga gaagaagagc gaagcctgct ggacgcagac
     2221 ctcctcgccg atatcgcgga agaacgcctc catgctcttt gagccgtcgg tcaggtgggc
     2281 gatgaaggcg ttgaggaagt tgagcccgct gacctggaac gcccgtttca gcggccgctc
     2341 gatcagcccg atcaggagga cgaagaccag cccgaagacc acctggagga ggatggcgta
     2401 ggggacgaac ccgggcgtga atatccaggc accgaccgct atgccggtca cgccctggag
     2461 gagcgccggg gcggccatcc gggtgatccg gtagtcggcc acggcgacga ggaccagcag
     2521 gcggatgccg gtggcaagcc cgaggacacc cgcgtagagc gcggaaaaga gttcccgccc
     2581 gaagaccagg accggcgaga ggctcagtat caccatgagg accgtgcagg caagggcaag
     2641 aaggcccgac cggttgtagg tgatctgacg gccaaagacc tccaccagcg gcacggtcag
     2701 caggaaggcg accagggccg ggagcgcaaa gccgagcgtg ccgaccagga agaacccgtt
     2761 gcccacccgg tatgcggcta tatcgatgac gaccccgagc aggatgatga gcagaagcga
     2821 ccgcggccag gacggagcgg agaagaggta gcgggtgagc cgctcgaaac gcacgtctgg
     2881 gctggactcc atcagatgcc cccgggagca ggagccggcg ataaagcgcc ggcattcagc
     2941 gcaatgcacc ccatgattca gggagatatt tcgcccggct caccaaagac cttccgatac
     3001 cggacaagca tccgctccca gtccggaagg tttgtctggg tgcccggccg ctcgacgacg
     3061 aaggacgaga ccaccgctcc cgcgcggcag cagtcgatcg gggcgtagcc cctccggtat
     3121 gccgtgagga acccggcccg gtaaccgtcg ccggcgccgg tggggtcgac ggcattcacc
     3181 ctgacggccg ggacgtggtg ctcctcgccg tccatgcaga ggacgctccc ttccgctccc
     3241 cgggtcgtga cgacgatcgg gaccgaggcg atgagcgccg ggcgttccag ccccagcata
     3301 tcgcacatcc tgtccatctc gtggttgttc gagaagagta tatcgatctt cgcgagtatg
     3361 atctcgagct ggtcgggggt gtagcggagg aggtcctggc ccgggtcgaa cgatgcgaac
     3421 cggctctttt cggccacccg gacgttgaag tcggggtcgg ccgtcgccat gtggacgaag
     3481 tcgagcgccg gggcctccgc ccgggagaac gcggcggacg ccccccactc aaagaaggtc
     3541 tcctggtcgc cggcctcgtc cgtgaagacg tatgcggtcg ccgtccgggc ctccttcacc
     3601 agggtgaagt cctgcacgat accgagatcg cgcatccagc ggtcgtagtc gctccccgga
     3661 aagtcgccgc cgaccggcga caccagccgg caccgttccc cgagcgtcgc gataccggcc
     3721 gcgatgttcg ccgccccgcc gccgaagtag acctggtggt cgacgatgta ggtcgagttg
     3781 tgcctcccgg ggaggcccgg gacagagaag aggtggtcga tggcggtatg cccgacgacg
     3841 gcaatcatac ctcctgcacc tcctggacct ttaagggcac gtcgcgcagc gccttcccga
     3901 cgaccgattt tgcgatccgg gatgcgtgtt cgccggactc cgcgcggaag accttcatct
     3961 ggagaacgag ccccacgagg gcggtgttcg cgaccacgag cgcgctgttg agttcaccct
     4021 cgcagtaggg gcaggcgatc atacccgcct cgatatccac gaacttcgcc gaggggttga
     4081 gccgtttccc cgcctcgctg atagcgatgc tgacggcgtc atccatagat ttgacatcct
     4141 tgattatcca cgctgattca agcgtaacca gatagtccgg catgagtctc tcctgttgat
     4201 aacctaccac gtctcgcggt gcacgtgctc ctccaccggc atccgctcca cgggcctgag
     4261 cggcggtatc cctttgccga ccgcaagaag cgcaaggggg cggatgtgct ccgggagccc
     4321 gagggtctcg cggacgtcgt tctcatcgaa cgctcccgtc cagcacgagt gcaggccgcg
     4381 ggcgtgggcg gcgagcatca tgtaggtgca ggcgatcgtg gcgtcctcga gcgcgtagag
     4441 gatcccccgc tccccgtact gcgacatgga cctgacgtag tttgcgcaga ccacaaagat
     4501 cgccggagct tcccggatat gcatctgctc gagggccgcg accgcaagcc ctatccgcag
     4561 atcctcgtcg gtgacgacga cgacgtccca ggcctcgcgg ttgccggcgc tcggcgccgt
     4621 gctcgcgcac gtgaggatgt agtcgatatc ctccgggtcg agcggttctc cggtgtagtc
     4681 ccggaccgac gaccgcgcct tgaggaggcg gaggagatca gaggaggtca tgctctttga
     4741 gcgtatccca cgccgtctgg gcggcggtcg tgacggcgct gacggctcgt gccatccgct
     4801 ccgacgcggc gccgagctcc atgctctcgg tcaggctgat cccctcgttg aggtgatcga
     4861 tggctttcct gaactcacgc tgttccgtgt cgccgtatgc aaactcaagc tccgagcgga
     4921 tagactggag caccatcaga agcatccgtt ttccgccggg tttctcctgg agcggaaact
     4981 ggatcagggc tgtcgtcagc tgcgatccga cgatcagctc ggacttcgcc cgttcggcaa
     5041 actggtaggt cctgacggcg gttttgagat ccataccaca tactggaact cgctactata
     5101 aaaagtattg aaagagaaga gagggagagc ttatctcgat ttctgcttgg tcccgagagc
     5161 cgaaaccttc gcgcgccgga tgctccggcg tacccgcacg tccatcgtgg gagtgccccc
     5221 gcctctggca ccgcgtccgc cacggccgcc gcgtccaccg cgtccaccac gccgctggtc
     5281 ctgtccttct ctgcttgccc gcttcctgat atccgccaga ggcttgaacc gctggttcgg
     5341 gccgggtttc tttacttcgc cttcgcttgc agggacgagc cgtatctgtc ccttcacgcc
     5401 cttaacctgg gcccacttta cgcttcctgt ctttcccatg gataaaactc ctttacaatt
     5461 atgagcggat ccgctgataa aagtaacccg gggccctgcc gcaacagggc aggatcccgc
     5521 cgcagatcag gagaggagac gcttgagctc gcgccggagg gtctcgctga cgaccttgcc
     5581 gtcgacgctc cccctgagtt cctgcatgac gacgcccata agggggccga gcgcgcccat
     5641 gcctttttcg cgggcgaacg cctcgcgttc ggccacaatc cggcgcacga tcgactccac
     5701 gtccgcctgc gagacggcgg gcgccatctt tgcgatggcc gcatccacgc gttcgcgggg
     5761 tgtcccggcg ctctcgtccg ccgtccgcgc gagttcggcg aggagatccg gtatcgcctc
     5821 ctttgcggcc cggccggcct ccacggcgga gaggagggcg aggatgtcgt cctcgctcac
     5881 ccggtcgatg gcgacgccgc cgcgggcaag ctcgcggcag gtggcaagga gcgtccgggc
     5941 cgcgagggtg gggcggacgc cggcggcgac ggccctctcg aagagcggca gccgttcgga
     6001 gaacgccacc tgccgggcca gcgccccgtc aagcccgaac tcccggacga accgctccgc
     6061 ccggtcggtg aggagttcgg ggaccctgat gccctcccag agggcgccgg agagcgctat
     6121 cgggaagacg tcggtctcgg gatacatccg ggcggccccg gggagggggc gcatgtaggc
     6181 ggagctcccc tcctccagca tcttccgggt ctcttcgggg acgccggcga gggccatgtc
     6241 cgcgcggatc atcacctgtt cggcagcaca cccggcccgc tcccggctcg ccgcaacgat
     6301 ggcgacgcag tcatcgtcgc cggcgccgac gaactcgcgg aggcgcgcca cctcttcggc
     6361 ggtgacgccg taggcgggga gttcgtcggt gtggaatatg ccgccgacac cgcacttctt
     6421 cgcgtagtcg gacatctcgc tgccgaggcg gcggccgggc tggatctccc gcccgacgag
     6481 ccctgcaaac ccacagagcc ggatcgcgag gatgaatttc gccttcttca gtacggcgga
     6541 cttcgtcccg gcaaagaggg ccgtgacgtc gacgacggtg cggtccaccc gggcgccgcg
     6601 atcccggagg gcgtcgcgga tggcaaggag gtttgtctgc cggtcgacct cgcgcctgac
     6661 cacctcggcg atgaggtcga gttcctggac gcccttgatc tcgacccgtg cgccgcctgc
     6721 tatggagacg ttgatgtcct ggcggatcgt cccgaggccc cgcttcaccc tgccggtcga
     6781 ccggagcacc ataccgatat atgcggcgac ttcctggacg gcctcgggtg tgcgcatgca
     6841 gggggcggtg gtgatctcga cgagcggtat gccgagacgg tcgagggaga aggtctcgcc
     6901 ctccacccgc tgcgccgcct cctcctcaag gcagatcgtc tcgatccggc acccgtcggg
     6961 gagggcgccg gagagagcga cgagcgccgt ccgctggaac ccgctggtgt tcgatccgtc
     7021 gacgacgagc tttctcatcg tgtggatctg ttcgacgggg gtcatgccga gcatcttcgc
     7081 tatcgtgagg cagatgtcga gcgcctcggg gttcatcggc gtcgggggct cctcgtcgtg
     7141 gtccacgaga cagaccgtgt cgtaggtgta gtagcagaac ttccggacga gtttcatctc
     7201 ctcttcggcc gcccggtcga tctccccgag ctcgctctcc gtcgcccgga ggtagcggta
     7261 gaactcgccc gtccggtcgg caacatcccg gaggacggtg gggcaccggc agaagagttt
     7321 ctcggcggtg tcgagctgct ggtggatctc gatgccggcc ttgaggccga ggtctttgta
     7381 atccattcag atcgacctcc gccggatctc gcccgcgaga tcggtctgca tcagcacgcc
     7441 ggcccgttcg gggtcgggct cgttcccgag cacccacatc agtttcacga gcgccacctc
     7501 ggggagcata tcctcgccct cgatgacgcc ggcgcggagg aggtctcttc cggtgttgta
     7561 gacccggtcg cagaccctcc cgtgcaggca ctgcgacgtc atgacgacgg tcgtcccgcc
     7621 ctcgatccta tcccggagtt tcgggatcca gtccgtcgca acgtgcccga gtccggtccc
     7681 gacgatgacg aggcccgcgt agccctcgaa ggcgtcgagg atgccgggcg gcatgccggg
     7741 gtagaactgg aggagggcgc agcgctcctc gagtgtatcg tggagcccgg gctcctccgc
     7801 cccccggcgc accgcctcat ccgagagggt gaccgcgagc gaggggtagt cgacggagcc
     7861 gagcggggcc atgcccatgc tctggaaagc gtcgcgccgg gatgtgtgca ttttgcggac
     7921 ccgcgtcgcc cggtggacgg cgcaccggtc gtcgtttgtc gtcgcgtgca tcaccaccgc
     7981 cacctcgccg agatcgccgg cggcaacagc ggcgctgcag agggtgttca tggcgttgtc
     8041 ggagctcggg cggtcggccg accgctgcga cccgacgaag acgaccggca ccggcgtcct
     8101 gagcatgaac ctgaccgccg cggccgatga cgccatcgtg tcggtgccgt gggtgacgat
     8161 gacgccggac gtgccgtgcc ggatctcgtc gtagatcgcc cgggcaaggg cctgccagat
     8221 cgcgggacgc atgttctcgg agaggatgct tgccacctgg cggtcgcggt agcgggcgat
     8281 atccccgagt tccgggatcg accggagtat gtcgtttgcc gagaactggc tcgtcaccgc
     8341 cccggtccgg tagtcgatcc ggcttgcgat cgtcccgccg gtggatatga tggagagttc
     8401 cgggagggcg gggtcctgga tgacgatgcc ggccccggcc ggcggctgcg gggctgcacg
     8461 cccgacccgt tcaagcttct cgggggatgc cccgatgttg tagccgctgt cgagcttgat
     8521 gacggccatg ccgtcccgct cggcgatgta gatgccggtg agtgcggtcc cgccgttggc
     8581 gtagcgcacc cggtctccgg cctcgagtga ttccgttata gtatcagcct ccgtgattcc
     8641 gatatgagat cttcaaaagc gcgagagagc gccgtatccg tctcctccgc ccatgccctg
     8701 tcccgggcga ggagttcttc ctgctcagta agctgcaccg cgaccgccgc gggtgccggg
     8761 ccgccgacga tcgtccgcac cgcgacggcg tggcgcgggt caagggcgag ggcgatccgc
     8821 tcctctgtca gcccgaggtc gacgaccgat aggccggccg cttcgcgggc ggctccttcg
     8881 agcgtcgcga tgtcgagcga accgtgccgg accgcccgcc cgacgatccg gtgggcggtg
     8941 cggaagggaa gtccgtactc cctgacgagg acgtcggcaa gttccgtcgc ggtcgagaac
     9001 cctctgcccg cctcggcggc catccgttcg gtgttgaagg ccgcggtggc gatcatgccg
     9061 gacagcaggg ggatgctctg gcgagcggcc tccacacccc gccagaggtg gggagtcagt
     9121 tcctggaggt cgcggttgta gctcatgggg agacccttca cgatggtgat cgccgcgata
     9181 agcgaaccgg cggccgaccc ggccctggcc cgcatgatct ccgcaacgtc ggggttcttc
     9241 ttctggggca tgatcgagga ggaggagcag taggcgtcgt cgaggtggac gaacccgacg
     9301 aacgcgctgc tccagatcac cagttcttcg cagaaccggc ttgccgtcgt catcagggcc
     9361 gcgatatccc cgagcacctc gattgcgaag tcccgggcgg cgaccgcgtc catgctgttt
     9421 ggcgccggcc gggagaagcc gaggagccgg gcggtgtagt cgcggtcgag ggggaaaccg
     9481 gtcgaggcga acgccgccga accgaggggg gatacgtcca cccgggcgta ggcctcccgg
     9541 agccgggcgg tatcgcggga gaaggcctgc tcgtaggcga ggaggtggtg ggcgagcgtc
     9601 gtgggctggg catgctgcag gtgggtgaag cccggcatca ccgtctccgt atgggcggcg
     9661 gcaacgtcga ggagggtccg ccggaggccg gcgagcgatt ggacgagggc gatgagttcc
     9721 tgcttcaggc gtatcctgat acaggtcgcg acctcgtcgt ttcgggaccg gcccatgtgg
     9781 aggcggccgc cgaagtcctc cccggcccgg ccgatgaggt aggcctcttt cccggcgtgg
     9841 atgtcttcgt agtgctcatc gaacgcctcc tccgggaggc cgtggtcgtg gaggtcgagg
     9901 agcgccgcca tcagcgcacg ggccggggct tcgtcgatga tcccctgccg cgagaggccg
     9961 aggaggtgcg ccatatccac gaggaggtcc gcctccgcga tccagcggtc ggcggccatc
    10021 gaggcgagaa aatgctcgag gtcggccgaa cgctcgccgg aaagacggcc ctgccgaatc
    10081 tgatccgaac gcatgctatc aacagtcctg tgtatcgttt ggcccgggaa gagattaaaa
    10141 ccacgcccgg ggtggggaac gggcggcgag ccggggctca cgcgggagga ggatgccggc
    10201 ggctgcgggt tggcggcaga gggcggcgag ccgggtcctg aaggcgtggg ggatagactg
    10261 gagaagagcc gggcacggcc gcccaccaga tatatgcccg ggcactgcac ggccccgggc
    10321 gggggcgggg gaggaggccg gaggcgtggg tggggtgggg acggccggcc tcgccttcgc
    10381 gctcttttgc ggaggcgccc cgcgccatct ctcgtctccg cgcacggcgg cagcgccccg
    10441 gccggcctcc tgcaccccca cctgatcatc cttaaaggat aattcataca ttaaaattaa
    10501 tactgaagta taacaataaa ccgatttttc ggtaacgcta tatatcgcca gcatgaagat
    10561 tacacacata ggagatacct atgagatcga tcacagcaac tcttctggtc gtggccatgg
    10621 tcgcggcgtt tgcgtgcatc gccgggtgca cgggcaccgg cgccgacgac atgacgcacc
    10681 tgcggatcgg ctaccagccg agcacccacc agatcgctca cttcaccgcg atggagaagg
    10741 gatggtggca ggaagacctc gcgcccctcg gcatcacaaa ggtgaccgac aaccagttcg
    10801 gcaccggccc gcccgagatg caggcaatgc ttgccggaga actcgatatc gcctacgtcg
    10861 gcgcagcacc ggtcgtatcc gccctggcaa gcggccttga cgcaaagatc atcgccgcag
    10921 tgcagactca aggatccgac ctcatgctca ggaacgacat cgcatacgag agccctcagg
    10981 acctcgtggg caaagagatc gccacctacc acccgggctc gatccaggat acactcctcc
    11041 ggacctggct tgagaagaac ggtgtcgacc ccgcgaaggt catcatcaaa cccatggacc
    11101 ccggtgcggc caccaccgcc atcgctgcgg gcaaggtcga cggcgtcttc ctgcccgatc
    11161 cctacccggc gatcatcata gcagagggca cggggaggac catcgtgcag tccggcgaga
    11221 tgatgaagga ccacgcctgc tgcgtcctcg ttgcaaccgg ctcccttatc cgcgaacacc
    11281 ccgacatcgt cgaacagatt gtccggactc acatcaaggc gacggagtac aacttcgaac
    11341 accaggatga ggctgcagag ctctactccg cgaagaccgg acagaacctc gataccgtga
    11401 aggcatcctt caagatctgg gacggcacct ggaccgccga cccgcggatc atcataccgt
    11461 ccacgatcga gtacacaaac ctccagtacg agcttggcta catcaagaag cccctgaccc
    11521 aggatgacct cttcgatctc tcgttctacg agaaggcccg ggcataaccc gggaccaaac
    11581 cccaccattt tccccccaaa gtatcttttg ctgcgatgtc gataaagtac tgaagaatat
    11641 accgggagga tcgatagaca attcctcccg gcaggaatac ctttgagcag cggaatcgag
    11701 acatgaatcg acagacacag aaacgactcc ttggcatcac agcgctcgtc accgtggccg
    11761 tgatctggca gatcatagcc gaatacgtgg tagggcgatc gttcatcctc ccgagcgtca
    11821 ccgatgtcat cagcgcattc gtagatctca tcgggtccgg cactctcctt accgatatca
    11881 ccgtaagcct tgaacacttc gggatcgggc tcctcgcggc gttcttcctc ggcgtcccga
    11941 tagggatact gatggggtgg ttccgaacgg cagacttcct ccttgacccg atcatcgagg
    12001 tcttccgccc cattccgccg cttgcctgga taccgttctc catcatctgg ttcggactca
    12061 ccacccaggc agcgggcttc atgatcttcg tcggagcctt cttccccatc ctgaccgcga
    12121 catacaccgc gttccggtcc ataccgaagg tctttgtcga ggcgggcaag gtgctcgggt
    12181 gcaacacatc gtttgaactg atccgccacg tcgccctccc cgccgccata ccggcgatcg
    12241 cctccggcat caggatagcg atgggcgtcg ggtggatgtg cctcgtaggt gccgagatgt
    12301 tcggcgtatc aaattacggc ctcggctacc agctctggca caactactac ctccaccaga
    12361 tgcccaacgt cgtcgtctac atgctgatcc tggggttcac gggcctcatc atcgaccgca
    12421 tcttccgaaa ctacgtggat ggacggctcc tgcggtggca cgcaggggag gtggcttaaa
    12481 tgggcgacgt atcgatacgg gacctcggca aggccttccc gaaggaggac ggcaccgtca
    12541 cgcaggcgct caagggcatc gacctcgaga tccgggacga agagttcatc tgcctggtcg
    12601 ggccgtcggg gtgcggaaag acgacgctct tacggatcat agccgggctt gagaccgcga
    12661 ccaccggaga ggtgaccgtc gacggccgcg ccgtcaccgg cccggacccg aagcggggca
    12721 tggtctttca ggagtactcc ctcttcccct ggcggcgggt gatcgacaac gtcgccttcg
    12781 gcctcgagat gaaaggagtc aagaaagaag aacgccacaa gacggcggat tactacatcg
    12841 acatggtcgg cctctcccag ttccgggatg cctaccccta cgaactctcc ggcggtatgc
    12901 ggcagcgggt cgcgatagca cgagcgctcg caaacgaccc cgacgtcctc ctcatggacg
    12961 aaccgttcgg ggcgctcgat gcccagaccc ggaaccggat gcaaaaagag ctcctctctc
    13021 tccgggacaa gacgaaacag acgatcgtct tcgtcaccca cagcgtcgac gaggcggtct
    13081 acctcgccga ccggatcatc gtcctctcgc cacggccggc gacggtccgc gaggatatcg
    13141 ccatcccctg gccacggccg cgggaccgga ccagtgccgg gttcgccgag gtccgccggc
    13201 gggtgctcga tatgatcaac gagacgggaa gcgtccagta aggtttatta gacaaaatcc
    13261 ccattttata aagcactcat agatcggcag gagtttttaa cgatggtacg aaaaccagcg
    13321 aagatgtaca ggaatctcgc aaagaaagca tatacacgca gggaatacat gggcggcgtg
    13381 ccgggcagca agatcgtgca gtttgatatg ggcaacctca cccaggactt cccggtcgaa
    13441 ctctccatcg tcgtcgaaga gacctgccag atacgccaca cggccctcga ggccgcccgt
    13501 atcggcatca accggcagct cttgaaggag gtcgggaggg caaactatca cttgaagata
    13561 cggaccttcc cccaccacgt cctccgcgag aacaagcagg cgaccggcgc aggagccgac
    13621 cgtgtctccg aaggcatgcg tcttgccttc ggaaaggccg tcggcaccgc ggcgcgggtc
    13681 cagagcggcc agaaagtctt ctccgtctgg acaacccccc agtacgccga gaaggcgaag
    13741 gtctctctca aacgcggcat ctacaagata ccgacccccg cacggatcgt cgaagagcag
    13801 accatggaag cgtaggcctg cacaacgccc aattcttttt tgcgccgaac ggttcgcaga
    13861 gggcggaact gaggccggcg ggaatagagt atttatagcc tcttcccacc agtaataata
    13921 atgactcatc cggcagaccc ggcgctcctg cgcgcgctcg ccaccgccac cgagaaggct
    13981 cttctagagg ccgagatccg gctccccacg gacgtcctcg cggcgctccg gagggcggcc
    14041 gcggccgaga agaacgaggt cgcccggcag gaattcgccc ttatcctcga gaacatcgct
    14101 ctcgccggag agcggcaggt gccgatctgc caggacaccg gcgtgccggt ggtctacctc
    14161 accctcccgc ccgacgttcc gttcacgccc gaccttttcg cgggaatccg ggaaggagtg
    14221 cgccgggcaa ccaccgcgat cccgctccgc ccgaacgtcg tcgatcccct gacccgggag
    14281 aacaccggcg acaacaccgg ggccggcatg cccgcggttc acgtcgtttt cggcgaccgg
    14341 ctcacggtga cggtcctccc gaagggcgcg ggctctgaga acgcatccag gatcgggatg
    14401 ctcctcccct cgcaggcagc ggatatcccc cggttcgtcg cggagacgat gctcatcgcg
    14461 ggcggcaaac cctgcccgcc ggtcttcctc ggcgtcggta tcggcgggac gtttgacggg
    14521 gccgcggcgc tcgcaaagga ggccctcctc ctcccggtcg acaccatgga cgactacgag
    14581 caggagatct gcgacgcggt caacgccctc ggcatagggc cgatgggtct agggggcgag
    14641 acgacggcgc ttgccgtgaa ggtgaagcgc agagactgcc acaccgcatc cctcccggtg
    14701 gcggtgaacg tccagtgctg ggcatgccgc cgggctaccg tggaggtgga gcggtgaacc
    14761 ttaccacacc gctcggcgac gaggtcctcg acctccgggc gggcgaccgg gtcaccctca
    14821 cgggaacgat ctacaccgca cgggacgagg cccacctccg catgatggaa gaaggcatcc
    14881 ccttcgaccc cgtgggcgcc gcggtctacc actgcggccc ggtggtccgg gacggccgtc
    14941 tcgtcgtcgc cggacccacc acatccgcgc ggatgaacgc tctcacgggg ttcctcatcg
    15001 acgcgggcgt ccgcgccctc atcggcaagg ggggaatggg gccggaggtg gtcgagcagc
    15061 tccgggggcg cgccgtctac ctcgcgttca cgggaggctg cgcagccctt gccgctgacc
    15121 ggatgagcct ctccggcgtc tattttgagg acctcggcat ggccgaagcg gtctggatca
    15181 tcgaggcgga tcacctcccg ctgacggtcg gcatcgacgc ccgcggcggc gatctcttca
    15241 gtgcagtacg cgagaaagcg aaaacacaat ttcaccagcg cttcaatatc aggtaggatg
    15301 ccgtctcatg aaacttgtca tcgatgaaag ccgctgcaag gggtgcaacc tctgcgtgct
    15361 ggtctgcccc tacgggatct ttcgagaagg gacggaactc aacagccggg ggatcgtctc
    15421 ccccgtcctc gaccgccccg agcggtgcac gaactgccgg ctgcaggctc tctacgggcg
    15481 gatgctctgc gggatctgcc acatgatctg ccccgaccag gccatctcct gggtcgagga
    15541 gaagccgttt gagccgcaca gggtggaggt ggagttttga gcagggttga gttcatgcag
    15601 gggaacgccg cctgcgcgga gggggcgctt gctgccgggt gccggttctt cggcggctac
    15661 ccgatcacgc cctcgaccga gatcgccgaa gcgatggccc ggcggctccc gaaggccggc
    15721 ggggtcttca tatccatgga ggacgaactc gcgagcatcg ccgcggtcat cggcgccgcc
    15781 tggacgggcg tccgggccat gaccgcgacg agcggcccgg ggttctctct gatgatggag
    15841 aacataggct acgcggccat gaccgagacg ccgtgcgtca tcgtcaacgt ccagcgaggc
    15901 ggcccgagca ccggccagcc gacgcgggcg gcgcagggcg acatgctcca gtgccggttc
    15961 gggtcgcacg gcgactacag catcatagcg ctcacgccga actccgtcca ggagacattc
    16021 gacctgacgg cgaaggcgtt cgatctcgcc gaccggttca gggttcccac cttcctgatg
    16081 accgacgaga tcgtcggcca catgcgggag cgggtgacca tccccgatgc ggtcgatatc
    16141 acccgctcct ccccgctcgc ggaaggctct ctaccgttcg aggccggcga cgacgggata
    16201 ccggggtttg cgcccttcgg ctcggggagg gcggtccacg tgaccgggct cacccacgac
    16261 gagcgcggct acccggacac gaccgatccc gcggcgcacg accgactggt gaggaggctc
    16321 gtcgcgaagg tcgagtcggc gcggcgcgct atcgccgact acgaggtcac gaaccccgac
    16381 gccgggacgg tcttcgtcac ctacggcccg ccgtcccgga cggtcgaaca ggtgatgcgc
    16441 gattaccccg acgacgccgt cggccacctc cggctcaggg tcgtctggcc gttcccggag
    16501 ttcgcgctcc acgagttccc gaacgcagag acctatatcg tgccggagct gaacatgggc
    16561 caggtggtcc gggaggtcga gcgccacgtc gaccggccgg tcgtccccct cggaaagatc
    16621 ggcggcgaac tccacacccc cgccgaactc gtccgggccc tggaggcgca ccgatgatcg
    16681 cgcccgactg gctcagggag gaccgcctgc cccacatata ctgcaccggg tgcggcaacg
    16741 ggacggtcat caactgcacc ctcgcggcgg tggaggagat gggctggaaa cgggacgaga
    16801 cggtcttcgt ctccggtatc gggtgctcgt cccgcgcccc cggctacata ctcaccgact
    16861 cgctgcacac cacccacggc cgggcgctcg cgttcgctac gggtgtgaag atggcgcggc
    16921 cggagttcaa cgtcgtcgtc ttcacgggcg acggcgacct tgccgccatc ggcggcaacc
    16981 acttcatcca cgcctgccgc cggaacgtgg acatgacggt ggtctgcatg aacaaccata
    17041 tctacggcat gaccggcggc caggggagcc cgaccacccc gccgggggcc atatcgacga
    17101 cgacgcccta cggcatgagc gaaccggcct tcgatcttgc ggaactcgcg gtcgccgccg
    17161 gcgcgaacta cgtcgcccgc tggacgtcct atcacgtcaa agagctcacg aaggccatca
    17221 ccgccggcat gcggacgccg gggtttgcgt tcatcgaggc aaggacccag tgcccgacga
    17281 actacggccg ccacaacaag ctcaggaccg tcccggccat gatcgaacac atccggagcc
    17341 acgcgatgct catcgagaag cgcgaccgcc tggtccgaga gggaagaccg gtcccggagg
    17401 atatcttcac catcggcgaa ctcgtccggc ggaaccggcc ggcgatgggg gtgcggcgat
    17461 gaggcacgag gtcaggttct cgggatacgg cggccagggg atcatcctct ccgcggtcat
    17521 actcgggcgg gcggcggcgc tctacgacgg gaagcacgcc gtccagacac aggtctacgg
    17581 cccggaggcc cggggcgggg catcgatggg acaggtggtg atcgacgatc gcccgatcct
    17641 ctaccccgag gtggccgacc ccgacatata cgccatcatg tcgcagcagg ggtttgagaa
    17701 gtacggggtc agggcccggc cggacgcggt catgctcctg gataccgatc tcgtccggtc
    17761 ccgcccggga tgccgctact atgagatacc ggcgacggcg gaggcaaaga ccgtcctcgg
    17821 ccgggagatc gtcgcaaaca tcgtcatgat cggcgccctc gtgacctcga ccggcatcgt
    17881 gagcagggag gcgatcgagc gtgcggtgct cgacagcgtc cccaagggca ccgagaacct
    17941 gaacatgaaa gcactgaaac gcggatttga actcggagag cgagcgtgta acttatgaaa
    18001 ctactggagt acgaggcaaa acagatcttt tcggagtacg gcataccggt cccgaaggga
    18061 gcgctcatac gggcgcccga agaggccgcg gcgcacctgc cggagacggg cggcgccgtt
    18121 gtgctgaagg cgcaggtgga cgtcggcggg aggggaaagg ccggcggcgt cctctttgcc
    18181 gatgcaaaga cggcggtcga taccgcccgc gaactcttcg cgcgggagat caagggcatc
    18241 ccggtcaggg aggtccttat cgaggagcgc ctcgcgatag agcacgagta ctacgtcagc
    18301 atcgccgtcg accggtcgag ccggcagccg gcggtcctct ttgccgacgc cggcggggta
    18361 gagatcgaga gtgcggcatc ggccgtccgg aaggtcatcg tatccccgct gctccgggat
    18421 ataccgccat tcctcatgcg cgaactcctc ggcggagccc cgaaggttct tgcgcccgtc
    18481 atcaaccgcc tctaccgcgt attccaggag aaagatgcca tgctcgcgga agtgaacccg
    18541 ctcgtgacga cgccgcaggg ggtctatgcg gcggacgcga aactgatcgt cgacgacaac
    18601 gccctcgccc gccaggggat agcggtcaac cgcgacctct cggagcgcga gcgcgaggcc
    18661 gagaagcacg ggttctccta cgtcgaactg gacgggacca tcggtgtcat cgggaacggc
    18721 gccggtctca cgatgtcgac gctcgacctg atcgagtttt acaacggccg ggcggcaaac
    18781 ttcctcgacg tcgggggcgg cgccgaccag gagcgcgtta tgcacgccgt ccggctcgtc
    18841 gcaagcatgc cggacgtctc ggtgatcgtc gtcaacctcc ttggcgggat cacccggtgc
    18901 gacgaagtgg caaagggcat catcgccgcc ggcgtcgccc cgacggtcat cgtccggatg
    18961 gccgggacga acgaggcgga aggccggcaa ctgctcggcg aacacggcta ccgcatgctc
    19021 gagagcatgg atgcggccgt ccgggcggcg atggaggtcg cgccatgata tacggcgata
    19081 agagcctcgg ggtgatcgtc cagaacatca ccggcaggca gggcatgttc cacacggagc
    19141 tgatgaacgc ctacgcccgc gatctcggcg gccggggtgt cgtcgccggt gtcgcgcccg
    19201 gcaagggggg ccgcgaggtc cacggcgtcc cggtctataa caccgtaaag gaggcgctcc
    19261 gcgaacacga tgcgaccaga agtgtcgtct tcgtgcccgg gggcgccgcg ggcgactcga
    19321 tcatggaggc ggcgcacgcg gggatcacgc ttgccgtcgt catcaccgag catataccgg
    19381 tccacgacgc gatgaaggcg atcgcctacg caaggctcca cgactgcacg gtcatcggcc
    19441 cgaactgccc cggcctcctc tcgcccgggg agtgcaaact cggcatcatg ccggcccatc
    19501 ttgccacccg cggtaatgtc ggcgtcgtct cccggagcgg caccctcacc tacgaggtgg
    19561 tcgacgaact cacccgcgcc ggcatcggcc agagcacggt cgtcggcata ggcggcgacc
    19621 ccgttatcgg ccagacgttc gtggacgtgc tcgcgcggtt cgaggacgat cccctgacaa
    19681 aggcggtcgt cgtcataggc gaggtgggcg gaaacctcga agaggagggg gtccggtcga
    19741 ccgatcttcc ggtcgtcacc tacatcgccg gcgtgagcgc tccccccgag aagaggatgg
    19801 gccacgcggg cgcgatcatc gagggcggcg agggcgacgc ccgctccaag gtgcggcggc
    19861 tggccgccct cggcataccg gtcgcgtccc ggccttcaga gataccgggg ctgatccgcg
    19921 aggtgctatg aacagcgacc tgatgctcgc caccgcctgc gaggtggcgg cgaagatcgg
    19981 cgcacgggcg g
//
LOCUS       DAOSII010000020        19499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_3051_length_19499_cov_48.307241, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000020 DAOSII010000000
VERSION     DAOSII010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 19499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19499
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3051_length_19499_cov_48.307241"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="PLV90_02810"
     CDS             <1..438
                     /locus_tag="PLV90_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOF96077.1"
                     /translation="CAAAMRERGLEPEDGLHNAVLGARKWAEELPPDPKVHFCSSVFK
                     DSVQLRERLRRIAANTARPFEEVTDDGTVVYGVLEPAGPLDGFLESLDEDDYVVCEGR
                     VEMAWWVLLDHGAGLPGKKYVVERYPNGGMVVEVTPLDAAIRD"
     gene            419..1189
                     /gene="uppS"
                     /locus_tag="PLV90_02815"
     CDS             419..1189
                     /gene="uppS"
                     /locus_tag="PLV90_02815"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOF96078.1"
                     /translation="MLRSGIDPLYERYLRWQVKHIPLHVAVIQDGNRRFARDQGLDRA
                     VGHRRGADTTEEFLDWACDLGVRHITLYTFSTENFRRDKDELAALFTLFQEKFAAIMT
                     DERVHRNHIRVQMIGDRSLLPDDLLATIDAAEEATLAYDDYYINIALAYGGRNEIVHA
                     ARSILDEVRQGAIDPATIGTTTVETHLHRGAPIPPVDLIIRTGNDCRTSNFLPWLANG
                     HESAVYFCAPYWPAFRRIDLLRAMRVYDQRMRLKERAA"
     gene            complement(1229..1819)
                     /locus_tag="PLV90_02820"
     CDS             complement(1229..1819)
                     /locus_tag="PLV90_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HOF96079.1"
                     /translation="MIYRLYEKYLLRDLSTLPQQICFMITEQDMLDAPGNLCLAAQWC
                     RDLGIGSVMFHISTADPARLARCLPLIRAVSSIGRLTLHYRDEKEVSGEGMHVTVAIG
                     KSGREEIACCVRRMAEDGIDPASVDEDLLESYLTFDYEPDLVIKTGGDHLTDFLIWQS
                     VYSELFFLDVNWALLRKVDFIRALRDFQSRARRFGR"
     gene            complement(1816..3483)
                     /locus_tag="PLV90_02825"
     CDS             complement(1816..3483)
                     /locus_tag="PLV90_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOF96080.1"
                     /translation="MRAPAGSVTNRDVAEQFLVMSRLLEIAGEDRYRSEAYGRASRAI
                     DRLALPVAGLDVEELVRIPGIGARIAGQIREIAETGSFDELDDMRAAVPASVVDLLGV
                     PGIGPRTLHILWKRLGILTVDDLERAVRGRRLRALRGFGPKREEAIERGIRQFRRRSN
                     RITRPQADAVLASVAALLCDGRYAVAGSYRRGASTVDGLDIVATGDRAALVRRVAAGE
                     SGDGVSFSLDGVPLNVRFTDPGRCGTALLGATGSDGFLARLGDVALARGYRFTPGGLV
                     DCSDGSLRAFASEEEVFSFLGMAFIPPELREDRGEVDLALRHALPDLIEIADIRGDLH
                     AHTTASDGRQSLEDVAEAGDARGYEYIAITDHSSRVGPEALARQQEAIERVNRRHTCE
                     LLCGSEVDIRSDGTLGYPDGVLADLDIVVASVHSGFAQDEDLLTRRILTAMENEHVDI
                     IGHPTGRLLGRRPPYAVDLVRVIEHAAVTGTALEINASPLRLDLEDIYVWRAKKQGVK
                     LAAGTDAHRTSEFSNMQNAVLQARRGWCAPGDVVNTLSLSELTEWVS"
     gene            3574..4305
                     /locus_tag="PLV90_02830"
     CDS             3574..4305
                     /locus_tag="PLV90_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOF96081.1"
                     /translation="MLPSTLEDYLEAIFTIAGDGGRSVGSQEVVAALGTEEAAAGATV
                     ASLVREGYLEQLADDTVRLTEKGLAVASRVARKHRVLQCFLTEMLGVDADAADKEACT
                     LEHGISDETIDRLSSYMDSAPPGHAGRCPACEICTLERETPDEAIDRSSMSDLRRPPG
                     RARHCRGRDACTLLDCKEGDTVRVAMIRGPRRNRRLLDLGIFPGEIVQIRRKLPNHSV
                     VVRVKGCDIAISPEIAMSIIVESCP"
     gene            4302..6152
                     /gene="feoB"
                     /locus_tag="PLV90_02835"
     CDS             4302..6152
                     /gene="feoB"
                     /locus_tag="PLV90_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOF96082.1"
                     /translation="MKFALIGNPSVGKSLIFNQLTGLGVEVSNYPGTTVELQRGNTCF
                     QREMIELVDLPGIYSLDGESGEEALVRRFLEQQDTDVVIAVLSATRLERNLYLLLQVA
                     EYGLPMVVVLNMVDEAAKQGLEIDPGPIGDLLGVEVILTAASQGKNIDRIIPAALAAT
                     RPVKVEVPYDHHIEAAIRSLGKISAADRKESIRALQGFGENPDLLEAAQTIAEEIESR
                     HRMTVAQIIAANRHNFAHQIAGLTIKEKTLHQKTDLDGILTRVIPGIPILIGILLGML
                     LVVFVVGSFLEELIVELFDIFLLQPFLALGLPPIVDALGTALILALQAGLGIAFPYVF
                     LFYIIISVLEDSGYMTRAAFLADNAMHRVGMHGGAVIPLTLAFGCNVPAIMSVRLLHS
                     RRERIIASFLVTMVPCSARTVIIAGIVAGFVGIGAAFSVYAIVFALIVLTGLVLSRIT
                     PGEQFGMIMEMVPLRRPDTRLVMRKAWMRLSEFLFIAMPLLLVGSVVLGLLEFFGIMA
                     IFEGFVEPYTMALLGLPGYSATALIFGILRKEMAFETLAILAGTADLGAVLSSLQLYI
                     FAVVTVLFIPCLATITVLLREVGSRITLAVTIYTVALGLFIGGLMYRILA"
     gene            6166..6891
                     /locus_tag="PLV90_02840"
     CDS             6166..6891
                     /locus_tag="PLV90_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96083.1"
                     /translation="MYRYEIGIPMIDREFGGLRAASNVLILAPPLTYAEHLAYRMACP
                     RAGEWTIALSTDERAAEVVGAFRKQGAQRNQVGIIDAVTKSSVPTLRDTAKAKFVTSP
                     LDLTSMGIKLSRMVEDMWREGVMADPPGPIPPPIRLCVNSVSTILMYARLEVTFKFLH
                     VITNRMKKFEGIGIYILNSESFDERTIATIKQLMNIVIEVRTEDGRQSVERDFRVIGV
                     RGRTTPWIRYFYDDGTLTIEEWR"
     gene            6917..7669
                     /gene="dph5"
                     /locus_tag="PLV90_02845"
     CDS             6917..7669
                     /gene="dph5"
                     /locus_tag="PLV90_02845"
                     /EC_number="2.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004164 - diphthine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0017183 - peptidyl-diphthamide biosynthetic
                     process from peptidyl-histidine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine synthase"
                     /protein_id="HOF96084.1"
                     /translation="MLTFVGLGLYDPEDISVKGLECVKSADVVYLESYTSRLMGTDVA
                     GMEAFFGKEVRVLAREDVEQDPRDIIEHALHGHVAFLTGGDPMVSTTHADLRIRAAAA
                     GVATAIVHASSISSAVCGLSGLQNYRFGKSCSVPFPAKGWFPTAPVETIAANLACNLH
                     TLVYLDIQKDRYMRIPEAIAIVEEMAAKRGIEPPALYVGIARAGSACPVVAAGTGADL
                     KETDFGPPLHILVVPGDLHPVEREYLEIFAGL"
     gene            7666..8235
                     /locus_tag="PLV90_02850"
     CDS             7666..8235
                     /locus_tag="PLV90_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF357 domain-containing protein"
                     /protein_id="HOF96085.1"
                     /translation="MTLEEQGALYLAALRRTRIAVPEGTLLYRAAEEVLEMAVAYQSD
                     GMAFLRAGDHVNALAGFAYGLGWLDAGSRLGLLLPLAAHPPGGAGVYIPEALAARLDE
                     KTHRYRRMLQSALPAIEVGPDEASPLSCGAEELRSTARCWYTDGVNRLDAGDPAGALA
                     RFSYGYAWLDAGIRAGLFRITGERGLFTV"
     gene            8413..9453
                     /locus_tag="PLV90_02855"
     CDS             8413..9453
                     /locus_tag="PLV90_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HOF96086.1"
                     /translation="MKKSQWKWLLVSISFSTLVLAGVLYFTVDETTIDYLSRVNTFYL
                     LLATLLHIVSLGFWALRIQAMSASLGYRVNFRHCLNLVFANMLVAAVTPSQAGGEPVR
                     IHELYRAGVPVGDATAVVVMERILDGIILGALGAFAMLFLGRYWSSLASGFSGISFIM
                     YAAWIAVTLFVLIFVYSVKNPDHLKRALRRLSCWIDRRWHLRRLEQLLGTIDREVDNF
                     NQGLVKFVNHGKSGLVWGLIFTTFFWFSEFIIASLLLVGLGQPPYFIESFVVQIVIAV
                     IMMIPLTPGGSGIAELGATSLYSLIIPSSIVGIFVLLWRLILYYLNILLGLLSSLVIV
                     RREFLARAEKQQ"
     gene            9500..9946
                     /locus_tag="PLV90_02860"
     CDS             9500..9946
                     /locus_tag="PLV90_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOF96087.1"
                     /translation="MTAQSCRVQGVFMSVSEMGAAPTVVLDAGGDSTIPIYVGLWEAI
                     SISNALNSEMLPRPITHDLVVELFRNFEIVLDALHIDSLEEGVFYAKLLLRQGSRTEV
                     MDCRPSDGIAIALRYRAPIMIEDTVVESAAVKKDDLPRMVDLKEYL"
     gene            complement(9979..10272)
                     /gene="hisE"
                     /locus_tag="PLV90_02865"
     CDS             complement(9979..10272)
                     /gene="hisE"
                     /locus_tag="PLV90_02865"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HOF96088.1"
                     /translation="MRDWSILDDLWLVIQDRAEHPSPESYVTSVLTHRKGIDKSLEKV
                     GEEAVEFILAAKNRVPERTVSEAADLLFHLLVALQASGTDVADVLDELAARRK"
     gene            complement(10265..10705)
                     /locus_tag="PLV90_02870"
     CDS             complement(10265..10705)
                     /locus_tag="PLV90_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusA-like transcription termination
                     signal-binding factor"
                     /protein_id="HOF96089.1"
                     /translation="MIRTICFKERRYIEELRILTRATALDCIIDERFDRIVYLIKEGD
                     MGLAIGRKGSNIRKMQRVLGKRIEMVEYSPEIESFTRNVFKPAEVAGVKKEDDGRLTV
                     YINKGDLGIAIGKGGCTIEKARILLSRYFDTELAGVLAREDVHA"
     gene            complement(10862..12304)
                     /locus_tag="PLV90_02875"
     CDS             complement(10862..12304)
                     /locus_tag="PLV90_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF96090.1"
                     /translation="MNCSQIPMGIFDHLRQPALVLDCEGRVVIWNESMERYTGIRAED
                     IVGEGGYAHGEALFGEKFPTLANRILSRGDAGDDTRYRLSAREDGKVIAGTCISPVSG
                     REVASMAAPLYDTAGRQIGAVETIQDTPDARPLRDPPGVPDEQAQILADALSCMIFTL
                     DWDGIYSRFFTTGAAPGSVPEEIVGKTPHALFPAAEADFLVEAARRVVEAGEVVAEMR
                     SFEWRGEQRSFEVTMQPLHDPKGRIVAAAGLSFDITRDLLRERALQEANQASSLYLDL
                     LGTDIYNTSMVAATVIEMLRERLSGEDAELAQRAKNTVEQGINVIKNVELLNTLNKHR
                     IRLEPVDLDEIIRGQIRRYAGIDIRYEGGSCMVWANPLLEHIVSNLISNSIKFGGMKV
                     RIDISVMETAEVVTLTVADTGIGIPDPLKPNIFDRFNRDARSVSGSRGLGLHIVKTLT
                     NQYGGRVWAADRVPGRPEAGAAIKVILQRC"
     gene            12875..13501
                     /locus_tag="PLV90_02880"
     CDS             12875..13501
                     /locus_tag="PLV90_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF432 domain-containing protein"
                     /protein_id="HOF96091.1"
                     /translation="MTYRRNCEGRTFERILISETGETIINPVEPVNLPGDVTDLFMVE
                     FSPMLIEPGITQTIYLKFPIEVGVFLEAAKDIEVLDIFGLGTQKYTLYGPPTSGAIAR
                     WYRSAVYPDIPAVERLREGVMELSIQNMSHEWVEVSCAVFDSVDMKIYYGDFVATTAT
                     MKIVTTHMAETDFIDAPLVPGMARSIELYTARKIPVIKRGYLMEWGLS"
     gene            13486..14358
                     /locus_tag="PLV90_02885"
     CDS             13486..14358
                     /locus_tag="PLV90_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOF96092.1"
                     /translation="MGVIVTFNVTEIFEIPIGVGSITVENLLYFAVIIIVGVAVAKTV
                     STNVRRALVERLPKNEREVLVKLVYYIIVTGALVIALPQLQFDLSGLLVAGGIVGLVI
                     GFASQSVVSNLVSGLFLMFEHPIKIGDSINVAGISGSVEDIRILSTVVKTYDGIFTRI
                     PNEKVFTSNIANYVHNAARRFEYQIGIRYEDDAEVAIRVIKEVIWTHPFALKNPAPSV
                     FVDNFGDDGVNLTAYIWAPTRNWWDVRTSLLWQIKQKLEENGIEMPFPQRTVRFADDL
                     RVTAGDAGKGGAED"
     gene            complement(14564..15226)
                     /locus_tag="PLV90_02890"
     CDS             complement(14564..15226)
                     /locus_tag="PLV90_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOF96093.1"
                     /translation="MFTITEVYNNIPCREGLTPDWGFSCLVEEAGLLFDTGAKGDVLL
                     SNMRALGIDPADIRYLVLSHDHWDHTGGVRAILGVNPSMEVFVHDAFTAQTLGMIGEY
                     TKPRIVREWMEIADGIAVTGPLGTDIREQSLAITAPGGYLVITGCAHPHIGRIVDRVS
                     QEGRVWGVVGGFHTVTTGDIGALAGVEYLSASHCTDAIPEIRERYPGTFRPGGVGKVH
                     RV"
     gene            complement(15348..16088)
                     /locus_tag="PLV90_02895"
     CDS             complement(15348..16088)
                     /locus_tag="PLV90_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844608.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOF96094.1"
                     /translation="MRGKAALLGIALMVLCAAVIGNVTAETPEESGDKLIHVSGTGKV
                     TTTPDQAIIVLAVEIENADAKVAQQQNAQKMDAVTNALKNAGIPAKDIRTAGYNIMPV
                     TEIDDKPLATSRVKFYRVTNNLEITLHDVDRAGEIVDIAVANGANRVDRLMLTLSDEK
                     QQQFRSEALTAAVKQARSDADAVVAALGKTVVDVKEVNVGSNYIPMAYDNRYLAATDM
                     AKGASAPTPVEIGEIDVTATVSIAYIIA"
     gene            16802..17884
                     /locus_tag="PLV90_02900"
     CDS             16802..17884
                     /locus_tag="PLV90_02900"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOF96095.1"
                     /translation="MTPVRLRAVGDVLLWTRNGKRPFDLVHHELQEKDLLFGNLETVL
                     SESGDPSRKRWVNTTPPEAAEYLEEAGFDILSLANNHTLDMGREGFEKTLDVLESHGI
                     AYIGGARGDGPPQALVVERRGIKLGFLAYTNGMFRSPPGVAVSRLKKKTVIDEIRRLK
                     DRCDHIIVSLHWGIENVYYPSPAQVRLAHDLIDGGATLILGHHSHTIQGLERYKDGLI
                     AYSLGNFQFDTGLFKEKIKSTMILSVDLDRGGIRDYTVIPCFINDDFQPEAAEGRIGE
                     KVERWITECSESVQNGRVTRRWWFEEIGEEYITYNLESYRYRIRQHGLAPLVECGVWL
                     VTPFCLNCYAGVIRRRLRERYTEGYA"
     gene            17935..19074
                     /locus_tag="PLV90_02905"
     CDS             17935..19074
                     /locus_tag="PLV90_02905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOF96096.1"
                     /translation="MARVAYDISRNLHENGHAVTVYTTNRSIYPSDLPTNRPTDVDGM
                     NVYYFENLRRYIPGATPPVIPYRMPAVAKRETARFDVIHIHDHRTLLTVIASHYARKY
                     GVPYVLQAHGALPDDTGSARMKRLFDVLWAKKVILGAAGVIALNETEAERYRNLGVDE
                     EKIAIVPNGIDLAEYPDLPPRGRFRAAWGIDDATKVVLYLGRLDPTKGIDLLIRSFGE
                     VGQEFGDAVLMLVGGDMGHNEEFRRRVRSLGLDDRVIFTGFVSREDKMAAFTDADVFV
                     TPSFTGFPITFLEACLCGTPIVTTGNGDLLGWVDNTVGFNTAYTPEALAGAIGRLLAD
                     DALRDGFSRQAKEIVRTRYNWQAIVHEIEALYATVANGYREPRGG"
     gene            complement(19174..>19499)
                     /locus_tag="PLV90_02910"
     CDS             complement(19174..>19499)
                     /locus_tag="PLV90_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96097.1"
                     /translation="NCTSINANCYGLQVDLAKNVRIENFHLENPAGINGKGASLGGTN
                     GQFGDSSVDIVASGDRVETLVWAKNNQNVEYSGEIRSGSARAFLVEGGGNVRTSGMKV
                     MPAGE"
BASE COUNT         3566 a         6227 c         6088 g         3618 t
ORIGIN      
        1 tgcgccgccg ccatgcgcga gcgcgggctc gaacccgagg acggattgca caacgccgtt
       61 ctcggcgccc ggaagtgggc ggaagaactc ccccccgacc cgaaagtcca cttctgctcg
      121 tcggtcttca aggactccgt ccagttacgg gaacggttaa ggcggatcgc cgccaacact
      181 gcgcgtccgt ttgaggaggt gaccgatgac gggacggtcg tctacggggt gctcgaaccg
      241 gccgggcctc tcgacggatt cctggagagc ctcgatgagg atgattacgt ggtctgcgag
      301 gggagagtcg agatggcgtg gtgggtgctc ctcgaccacg gggcaggact gccgggcaag
      361 aagtacgtcg tcgaacgcta cccaaacggc ggcatggtcg tggaggtgac cccgctcgat
      421 gctgcgatcc gggattgacc ctctctatga acgttacctg cgatggcagg tgaaacacat
      481 cccccttcac gtcgcggtga tccaggacgg caaccgccgg tttgcccggg accaggggct
      541 ggacagggcg gtcggacacc ggcgcggcgc agataccacg gaggagttcc tcgactgggc
      601 atgcgacctc ggcgtccggc acatcacgct ctacaccttc tcgaccgaga acttccggcg
      661 ggataaggac gagctggcag ccctcttcac cctcttccag gagaagttcg ccgccatcat
      721 gaccgacgag cgggtgcacc gcaaccacat acgggtacag atgatcgggg accgctccct
      781 cctccccgac gacctgcttg caaccatcga cgccgccgag gaggcgacgc tcgcctacga
      841 cgactactac atcaacatcg ccctcgccta cgggggccga aacgagatcg tgcacgccgc
      901 ccggtcgatc ctcgatgaag tccggcaggg cgcgatcgac cccgccacca tcggcaccac
      961 gaccgtcgag acccatctcc accggggagc ccccatcccc cccgtcgacc tgatcatccg
     1021 caccggcaac gactgccgga cgtcgaactt cctcccctgg ctcgcaaacg gccacgagtc
     1081 ggccgtctac ttctgcgctc cctactggcc ggcgttcagg aggatcgacc ttttgcgggc
     1141 gatgcgcgtc tacgaccagc gcatgcgctt gaaagagcgc gcggcctgac ggtcgccgcc
     1201 cgaaccggcg agatacgccc ggaacagatc accgcccgaa ccggcgtgcc ctggactgaa
     1261 agtcccgaag cgcccggatg aagtccacct tccggagaag cgcccagttc acgtcgagga
     1321 agaagagctc ggagtagacc gactgccaga tgaggaagtc ggtcaggtgg tccccgcccg
     1381 tcttgatgac gaggtcgggc tcgtagtcga aggtgaggta ggactcgagc aggtcctcgt
     1441 cgaccgacgc cgggtcgatg ccgtcctcgg ccatccgccg gacgcagcag gcgatctcct
     1501 cccgcccgct cttcccgatc gccaccgtca catgcatgcc ttccccgctg acctccttct
     1561 cgtcacggta gtggagcgtc aggcgcccta tcgaagagac cgcgcgaata agcgggaggc
     1621 accgggccag ccgggcagga tcggccgtgc tgatgtggaa catgacgctt ccgataccga
     1681 gatcgcggca ccattgggcg gcaagacaga ggttgcccgg cgcgtcgagc atatcctgct
     1741 cggtgatcat gaaacagatc tgttgaggga gcgtgctgag gtctcggagg aggtacttct
     1801 cgtagagccg gtagatcatg acacccactc cgtcaactcc gacagggaga gggtgttcac
     1861 gacgtcgcca ggggcgcacc agccccggcg cgcctgcaag acggcgttct gcatatttga
     1921 aaactcgctc gtccggtgag catcggtccc tgcagccagt ttcactcctt gtttctttgc
     1981 acgccagaca taaatatcct caagatcgag acggaggggc gacgcgttga tctcaagcgc
     2041 agtccccgtc acggccgcat gctcgatcac ccggacaagg tcgaccgcgt aaggcggccg
     2101 ccgcccgagg aggcgcccgg tggggtgacc gatgatatcg acgtgctcgt tctccatcgc
     2161 ggtgaggatg cgccgggtca ggagatcctc gtcctgggca aaccccgagt ggacgctcgc
     2221 gacaacgatg tcgaggtcgg caagcacccc gtcagggtag ccgagcgtgc cgtcgctccg
     2281 tatgtccacc tcgctcccgc agaggagctc gcaggtgtgg cgccggttga cccgctcgat
     2341 agcttcctgc tgccgggcga gggcctcagg gcccacccgt gacgagtggt cggtgatcgc
     2401 gatgtactcg tagccccggg catcccccgc ctccgccaca tcctcaagcg actggcggcc
     2461 gtcgcttgcg gtggtgtggg cgtgcaggtc ccccctgata tcggcgatct cgataagatc
     2521 ggggagcgcg tggcggaggg caaggtcgac ctcgccccgg tcctcgcgca gctccggagg
     2581 gatgaacgcc ataccgagga acgaaaagac ctcctcctcg ctcgcgaacg cccgcaggct
     2641 gccgtccgaa caatccacaa gaccgccggg cgtgaaccgg tagccgcgag caagcgccac
     2701 gtccccgagg cgggccagga acccatccga gccggtcgcc ccgaggagcg ccgtgccgca
     2761 ccggccggga tcggtgaacc ggacgttcag gggcaccccg tcgagggaga acgagacccc
     2821 gtccccgctc tcgccggctg cgacacggcg cacgagggcg gcccggtcac cggtggcgac
     2881 gatatcgagc ccgtcgacgg tgctcgcccc ccggcggtaa ctccccgcaa ctgcataccg
     2941 accatcgcac aggagcgccg ccacgctcgc gaggacggcg tcggcctgcg gccgggtgat
     3001 ccggttcgac ctccggcgga actgcctgat gccgcgctcg atcgcctcct ccctctttgg
     3061 gccgaacccc ctgagcgccc ggagccggcg gccccgcacc gcccgctcca ggtcgtccac
     3121 ggtcaggatc ccgagccgtt tccagaggat gtgcagcgtc cgcggcccga tgccgggaac
     3181 gccgagcagg tcgacgaccg atgccggaac ggcggcccgc atatcgtcga gctcgtcaaa
     3241 cgacccggtc tccgcaatct cccggatctg cccggctatc cgggcgccta tacccgggat
     3301 gcggacaagc tcctccacgt cgagcccggc gaccggaaga gcgagccggt cgatcgcccg
     3361 cgacgcccgc ccgtaggcct cgctccggta gcggtcctct cccgcaatct caagcaggcg
     3421 gctcataacg aggaactgtt cggcaacatc cctgttcgtg accgatcctg ccggtgccct
     3481 catgatcacc gatcgttcgc aataccatat agccgttgac accggtggca ccatccaaat
     3541 ccataaactg ctggaagtaa aagagattat atcatgcttc cttccacact cgaggattat
     3601 ctcgaagcaa tcttcacgat cgcgggagac ggaggacgat cggtcggatc gcaagaggta
     3661 gtcgcagcgc tcggcaccga ggaggccgcc gccggggcga ccgtcgcctc cctggtcagg
     3721 gaaggatacc tggagcaact ggcggacgac accgtcaggc tgaccgagaa ggggctcgcg
     3781 gtcgcttcac gggtggcgcg gaaacatcgc gtcctccagt gcttcctgac cgagatgctc
     3841 ggcgtcgatg cggatgcagc ggataaagag gcctgcacgc tcgagcacgg catatccgat
     3901 gagacgatcg accgcctctc ctcctacatg gacagcgccc cgcccgggca tgccgggcgc
     3961 tgcccggcat gcgagatctg cacgctcgaa cgcgagaccc ccgacgaagc gatcgaccgg
     4021 tcctccatga gcgatctccg gcgcccgccc gggcgtgccc ggcactgccg ggggcgcgat
     4081 gcctgcacgc tcctcgactg caaggaaggg gataccgtcc gggtggcgat gatccggggg
     4141 ccccggcgga accggaggct gctcgacctc ggcatcttcc cgggcgagat cgtgcagatc
     4201 cggcggaaac tcccgaacca ttcggtcgtc gtcagggtga aaggctgcga tatcgccatc
     4261 agccccgaga tagccatgtc catcatcgtg gagtcgtgtc catgaagttt gcgctgatcg
     4321 gcaaccccag cgtcggcaaa tccctgatct tcaaccagct caccggtctc ggggtggagg
     4381 tgagcaacta ccccgggacc accgtcgaac tgcagcgggg caacacctgt ttccagcgcg
     4441 agatgatcga actcgtggat cttcccggca tctactcgct cgacggggaa tcgggcgaag
     4501 aggctctggt ccgccggttc ctggagcagc aggacaccga tgtggtcata gcggtcctga
     4561 gcgccacgcg cctcgagcgc aacctctacc tcctcctcca ggtggccgag tacggcctcc
     4621 cgatggtcgt cgtcctgaat atggtcgatg aggctgcaaa acaggggctc gagatcgacc
     4681 ccggccctat cggcgacctc ctcggcgtcg aggttatcct gacggccgca tcgcagggga
     4741 agaacatcga ccggatcata ccggcggccc ttgccgccac ccggccggtg aaggtggagg
     4801 tcccgtacga ccaccacatc gaggccgcca tccggagtct cggaaagata tcggcggccg
     4861 accggaaaga gagcatccgg gcgctccagg ggttcggcga gaaccccgac ctgctcgagg
     4921 cagcacagac gatcgccgaa gagatcgagt cgaggcaccg gatgaccgtc gcccagatca
     4981 tagcggccaa ccggcacaac ttcgcccacc agatcgccgg cctcaccatc aaagagaaga
     5041 cgctgcacca gaagaccgac ctcgacggca tcctgacgcg ggtcatcccg ggtataccga
     5101 tcctcatcgg gatcctcctc gggatgctcc tcgtggtctt cgtcgtcgga tcgttcctcg
     5161 aggagttgat cgtggagctc ttcgatatct tcttactcca gcccttcctc gcgctcgggc
     5221 tgccgccgat cgtggatgcg ctcgggacgg ccctcattct cgccctccag gcaggcctcg
     5281 gcatagcgtt cccgtacgtc ttcctcttct acatcatcat atccgtcctt gaggattcgg
     5341 gatacatgac ccgggcggcc ttcctcgccg acaacgccat gcaccgggtc ggcatgcacg
     5401 gcggggcggt catccccctc accctggcgt tcgggtgcaa cgtgccggcc atcatgagcg
     5461 tgcggctcct ccactcccgc cgcgagcgca tcatcgcatc gttcctggtc acgatggtcc
     5521 cctgctcggc ccggaccgtc atcatcgccg ggatagtggc gggtttcgtc ggcatcgggg
     5581 cggcgttcag cgtctacgcc atcgtctttg ccctgatcgt cctcacgggg ctcgtcctct
     5641 cccgcatcac cccgggcgag cagttcggta tgatcatgga gatggtgccg ctccgccggc
     5701 cggacacgcg gctggtgatg aggaaggcct ggatgcgcct ctccgagttc ctcttcatcg
     5761 cgatgcccct cctcctcgtg gggagcgtcg tcctcgggct gctcgagttc ttcggtatca
     5821 tggcgatatt cgaggggttc gtggagccct acaccatggc cctcctcggc ctgcccggct
     5881 actcggcgac ggcgctcatc tttggaatcc tcagaaaaga gatggcgttt gagacccttg
     5941 ccatcctcgc ggggaccgcc gacctcgggg cggtgctttc gtcgctccag ctctacatct
     6001 tcgcggtcgt gaccgtcctc ttcatcccct gtcttgcgac catcacggtc ctcctgcggg
     6061 aagtcggctc acggatcacg cttgcggtca cgatctacac cgtcgccctc ggcctcttta
     6121 tcggaggcct tatgtatcgt atcttagcat aagtgatgtg ctgacatgta ccgttacgag
     6181 atcggcatac cgatgatcga cagggagttc gggggcctgc gggccgcctc gaacgtcctc
     6241 atcctggcac cgcccctgac ctacgccgag caccttgcct accggatggc ctgcccccgg
     6301 gcgggggaat ggacgatcgc cctctcgacg gacgagcgtg cagccgaagt cgtcggtgcg
     6361 ttccggaaac agggcgcgca gaggaaccag gtagggatca tcgatgcggt cacgaagagt
     6421 tcggtgccga ccctgcgcga caccgcaaag gcgaagtttg tcacgagccc tctcgacctg
     6481 accagcatgg ggatcaaact ctcccggatg gtcgaggata tgtggagaga aggggtgatg
     6541 gccgatcccc cggggccgat ccccccgccg atccggctct gcgtcaactc ggtctcgacc
     6601 attctcatgt acgcgcgcct tgaggtgacc ttcaagttcc tccacgtcat cacgaaccgg
     6661 atgaagaagt ttgaagggat cggcatctac atcctcaaca gcgagtcgtt cgacgagagg
     6721 acgatagcca cgataaagca gttgatgaac atcgtcatcg aggtgaggac ggaagacggg
     6781 cggcagtcgg tggagcggga cttccgcgtc ataggcgtcc gcggcaggac gaccccctgg
     6841 atccgttact tctacgacga cggaacgctg acgattgagg aatggcgttg ataccctatc
     6901 ccggagcacg cctctcatgc tgacgttcgt gggcctcggc ctctacgacc cggaagatat
     6961 atcggtcaaa gggctcgagt gcgttaaaag tgccgatgtc gtctacctgg aatcgtatac
     7021 gtcgcggctg atggggacgg acgttgccgg gatggaagcg ttcttcggca aagaggtccg
     7081 ggtgctcgcg cgcgaggacg tcgagcagga cccccgcgat atcatcgagc acgccctcca
     7141 cggccatgtg gcgttcctga ccggggggga ccccatggta tcgacgacgc acgccgacct
     7201 ccggatacga gcggccgccg ccggcgttgc gaccgcgatc gtccacgcct cgtccatatc
     7261 gagcgccgtc tgcggcctct cggggctcca gaactaccgg ttcggaaaat cctgctcggt
     7321 gccgttcccc gcgaaggggt ggttcccgac ggcgcccgtc gagacgatcg cggccaacct
     7381 tgcctgcaac ctgcacacgc tcgtctacct cgatatccag aaggaccgct acatgcgcat
     7441 cccggaagcg atcgcgatcg tcgaggagat ggcggcaaag cgcggcatcg agccgcccgc
     7501 gctctacgtg ggcatcgccc gggcggggtc ggcgtgcccg gtggtcgcgg cgggtaccgg
     7561 cgcagacctc aaagagacgg actttggacc cccgctccac atcctcgtcg tgcccggaga
     7621 cctccacccc gttgagcggg agtacctgga gatcttcgcc ggcctatgac ccttgaagaa
     7681 cagggtgccc tctacctcgc ggctctccgc cggacccgga tagccgtccc ggaggggacg
     7741 ctcctctacc gggcggcgga ggaggtgctc gagatggccg ttgcgtacca gagcgacggc
     7801 atggcgtttc tccgggcggg cgaccacgta aacgctcttg ccgggtttgc atacgggctc
     7861 ggctggctgg acgcaggctc gcggctcgga ctgcttcttc cgctcgccgc ccacccgccg
     7921 ggcggggcgg gcgtgtacat accggaagcg ctcgcggccc gcctggacga gaagacccac
     7981 cgctaccgtc ggatgctcca atccgccctc ccggcgatcg aggtggggcc ggacgaggca
     8041 agcccgctct cttgcggcgc ggaggaactc cgttcgacgg cgcgttgctg gtacacggac
     8101 ggtgtaaatc gcctcgatgc cggcgaccct gccggcgccc tcgcccggtt cagctacggg
     8161 tatgcctggc tcgatgccgg catccgggcg ggactcttcc ggataaccgg cgagcgcggc
     8221 ctctttacgg tctgagagaa tcgcctcccg cagcgaaccg gagatatcgc cgaatcgcga
     8281 ccgaaaatca tcgttaaata gtagccacac gataccttat tagtccaatt ccgggtaatt
     8341 ctctcctgaa tctgcccgaa atcatcaacg aaccgtgggt gtcaccggac accgatccgg
     8401 ggagtacaga gtatgaagaa gtctcagtgg aagtggctgc tcgtctcgat cagcttcagc
     8461 accctcgttc tcgcgggcgt cctctacttc accgtcgacg agacgacgat cgactacctc
     8521 agccgcgtaa acacgttcta cctgctcctt gccaccctcc tccacatcgt ctcgctcggg
     8581 ttctgggcgc tccggataca ggcgatgtcg gcgtcgcttg ggtaccgggt gaacttcagg
     8641 cactgcctga acctggtctt cgcgaacatg ctcgttgcgg cggtcacccc gtcacaggcc
     8701 ggcggcgaac cggtccggat ccatgaactc taccgggcgg gcgttccggt cggggacgcc
     8761 accgccgtcg tcgtcatgga gcggatactc gacgggatca tcctcggggc gctcggggct
     8821 tttgccatgc tcttcctcgg caggtactgg agcagccttg cctcggggtt cagcggcata
     8881 agtttcatca tgtacgctgc ctggatagcc gtcaccctct tcgtcctgat cttcgtctac
     8941 tcggtgaaga accccgacca cctcaaacgg gctctccggc ggctctcgtg ctggatagac
     9001 cggcgctggc acctgcggcg gctcgagcag ctcctgggca cgatcgaccg ggaagtggac
     9061 aacttcaacc agggactcgt caagttcgtg aaccacggca aaagcggcct cgtgtggggg
     9121 ctgatcttca cgacgttctt ctggttctcg gagttcatca tcgcatcgct cctcctcgtg
     9181 ggcctcgggc agccgccgta cttcatcgag tcgttcgtcg tccagatcgt catcgccgtc
     9241 atcatgatga tcccgctcac gccggggggc tcgggcattg cggagctcgg cgccacgtcg
     9301 ctttacagcc tcatcatccc gtcgtcgatc gtgggcatct tcgtcctcct ctggcggttg
     9361 atcctctact acctcaatat ccttctcggc ctgctctcga gtctcgtcat cgtacggcga
     9421 gagttcctcg cccgtgccga gaagcagcag tgatcgaccc caaggtttaa acatcataag
     9481 actcatgtca gatgatcaca tgactgctca aagttgcagg gtgcagggcg tgttcatgtc
     9541 ggtgagcgag atgggggcag cgccgactgt cgtcctggat gccgggggcg acagcacgat
     9601 accgatatac gtcgggctct gggaggcgat ctcgatcagc aacgcgctca acagcgagat
     9661 gctcccccgg cccatcaccc acgacctggt cgtcgaactc ttccggaact ttgagatagt
     9721 gctcgatgcc ctgcacatcg attctctgga ggagggggtt ttttacgcca aacttctcct
     9781 ccggcagggc tcgcgcaccg aggtcatgga ctgccggcca agcgacggta tagccattgc
     9841 tctgcgttat cgggcgccga tcatgatcga ggatacagtc gtagagtccg cggcggtgaa
     9901 gaaggacgat ctcccgagga tggtagacct gaaggagtac ctctgagcga tcgccccggg
     9961 ggagagggaa gcttgccgtt acttccggcg tgcggcgagc tcgtcgagca cgtccgccac
    10021 gtcggtgccc gatgcctgga gcgccacgag gagatgaaag aggagatcgg ccgcctcaga
    10081 taccgttctt tccggaaccc ggttctttgc ggcgaggatg aactcgaccg cctcttcgcc
    10141 gaccttctcc agggacttgt cgatcccttt ccggtgggtc aggacggagg tgacgtagct
    10201 ctcgggcgac gggtgctcgg cgcggtcctg gatgaccagc cagagatcgt cgaggatgct
    10261 ccagtcacgc atggacgtcc tcccgtgcca gcaccccggc aagttcggtg tcgaagtacc
    10321 gggagaggag gatgcgtgct ttctcgatgg tgcatccccc tttccctatg gcgatcccga
    10381 gatcgccctt gttgatgtat acggtgagtc tcccgtcgtc ttccttcttc acgccggcga
    10441 cctccgccgg tttgaacacg tttctcgtga atgattcgat ctcgggagag tattcgacca
    10501 tctcgatgcg cttcccgagg actcgctgca tcttccggat gttgctccct ttccggccga
    10561 tggcaaggcc catatctcct tcttttatga ggtatactat gcgatcgaag cgctcgtcga
    10621 tgatgcagtc cagcgccgtc gcccgggtga ggattcgcag ctcttcgata tatcgtcgtt
    10681 ctttaaaaca tatggttcgt atcattggag aagtctccga tgcgaatgat gcggtatagc
    10741 atagtcatca ggaggttata tatctgagta ccgggatcgc ctgccggcag gcgatccggg
    10801 ctgtcatggg atccgcgaag cgatggcacg ggatctgcag gttttcgggc ggcgcggggc
    10861 gctaacacct ctgcaggatc accttgatgg ccgctcccgc ctcgggtctg ccgggcaccc
    10921 ggtccgccgc ccagacgcgc ccgccgtact gattcgtgag ggtcttgacg atgtgaagcc
    10981 ccagacccct gctcccggat acgctcctcg cgtcacggtt gaaccggtcg aatatattgg
    11041 gctttaaggg gtccggtatg ccgatcccgg tgtcggcgac ggtgagggtc accacctccg
    11101 cggtctccat gacggatatg tcgatccgca ccttcatgcc gccgaacttg atgctgttgc
    11161 tgatcaggtt ggatacgata tgctcgagga gggggtttgc ccagaccatg cagctccccc
    11221 cctcgtacct gatgtcgatg ccggcatagc gtcgtatctg gccgcgtatg atctcgtcga
    11281 gatcgaccgg ttcaaggcgg atgcggtgtt tgttgagcgt gttcaagagt tcgacgttct
    11341 tgatgacgtt gatcccctgc tcgaccgtgt tcttggccct ctgggcgagc tccgcatctt
    11401 cgccggagag tcgttcccgg agcatctcga tgaccgttgc cgcaaccatg ctggtgttgt
    11461 agatgtcggt gccgagcagg tcgaggtaga ggctggaggc ctggttggcc tcctggaggg
    11521 cccgttcgcg gaggaggtcc cgggtgatat caaagctgag accggcggca gccacgatcc
    11581 ttccttttgg gtcatgcagg ggttgcatgg tgacctcaaa cgaccgttgt tcgccacgcc
    11641 actcaaacga ccgcatctcc gccacaacct cccccgcctc gacgacccgg cgtgcggcct
    11701 ctaccaggaa gtccgcctcc gccgccggga agagggcgtg gggggtcttc ccgacgatct
    11761 cctccgggac cgatccgggg gcggcgccgg tcgtgaagaa ccgggagtag atgccgtccc
    11821 aatcaagcgt gaagatcatg caggagaggg catccgcgag gatctgcgcc tgctcatccg
    11881 gcaccccggg gggatcgcgg aggggtcggg cgtccggcgt atcctggatc gtctccaccg
    11941 cgccgatctg tctgccggcg gtgtcgtaga ggggggcggc catgctggcc acctccctgc
    12001 cggataccgg actgatgcat gttccggcga tcacctttcc atcttcacgg gcagagaggc
    12061 ggtagcgggt gtcgtctccg gcgtcgcccc gggagaggat gcggtttgca agcgtaggga
    12121 acttctcgcc gaacaacgcc tccccgtgcg catatcctcc ttccccgacg atatcttcgg
    12181 cccggatccc cgtgtatcgt tccatgctct cgttccagat gaccaccctt ccttcgcagt
    12241 cgagaaccag tgccggttgt cggaggtgat cgaagatccc catgggtatc tggctgcaat
    12301 tcacggattc tctggtattc tcgtggttta ccttcacggg tatcacccgg ggccgatcgt
    12361 gcctcccaca accgcaaagg agtcctctcc tccgggaggg ttcttcgcag cggcaggtac
    12421 ggcgccccca tatctgtgct cccggtcccg gtaccccgtt ctcccgcgac agcagccccg
    12481 gatggtggcg aaggataggg gtgcttgccc ggggaccgga acagatgcac gttaaaaaat
    12541 gtctgggatg accgcttaaa aggatgtcta atgatgcccc gaagaccgtg cttcccggaa
    12601 ggcctgatat ttatgccttg ccggatcgct cccgggcata atggccgggg ttgatacaat
    12661 gaattggttt catggaacgg cacgaccgaa agaccgatat gctccgtcat caacgatcgg
    12721 gtgcagggtc gcgaacgtcg gcaggtcgcg gccggtcgcg taccttaata tttgattatt
    12781 tgcagatcat agagtgatgt tcgggcgtta ctctggcact ttccgccacg aaaatggcga
    12841 tattctggta gaaaccgagc ggaacggcgg ttttttgacc taccggcgga actgcgaagg
    12901 gcggacgttc gagcggatac tgatctcgga gacaggcgag accatcatca acccggtcga
    12961 accggtcaac cttcccgggg atgtcacgga tctctttatg gtagagttct ctccgatgct
    13021 catcgagccc ggtatcacgc agacgatcta cctgaagttc cccatcgagg tcggcgtctt
    13081 ccttgaggcg gcaaaagata tcgaggtgct cgacatattc ggcctcggca cccagaagta
    13141 cacgctctac ggcccgccga ccagcggcgc catcgcccgc tggtaccgga gcgcggtcta
    13201 ccccgatatc cccgctgtcg agcgtctccg cgagggggtg atggagctct ccatacagaa
    13261 tatgtcccac gagtgggtcg aggtctcgtg cgccgtcttt gacagcgtcg atatgaagat
    13321 ctactacggc gacttcgtgg cgaccacggc cacgatgaag atcgtcacca cccatatggc
    13381 ggagacggac ttcatcgacg ccccgctcgt gcccggtatg gcgcgatcca tcgagctcta
    13441 cacggcacgg aagataccgg tgatcaaacg agggtatctc atggaatggg ggttatcgtg
    13501 acgttcaacg tgaccgagat cttcgagatc cccatcggcg tcgggtcgat caccgtcgag
    13561 aacctcctgt acttcgccgt catcatcata gtcggcgtcg ccgtcgcaaa gacggtctcg
    13621 acaaacgtcc ggagagccct cgtagagcgg ctcccaaaga acgagcgcga agtgctcgtg
    13681 aaactggtct actacatcat cgtcaccggg gcgctcgtca tcgccctccc gcaactccag
    13741 ttcgacctct ccggcctcct ggtggccggg ggcatcgtcg gtctcgtcat cgggtttgcg
    13801 agccagagcg tcgtctcgaa cctggtctcg ggcctcttcc tcatgttcga gcaccccatc
    13861 aagatcggcg acagcatcaa cgtcgcgggc atcagcggca gcgtcgagga tatacgaatc
    13921 ctctcgacgg tcgtcaagac gtacgacggc atattcacca ggatcccgaa cgagaaggtc
    13981 ttcacgtcga acatcgccaa ctacgttcac aacgccgccc gcaggttcga gtaccagatc
    14041 ggcatccgct acgaggacga cgccgaggtg gcgatacggg tcatcaaaga agtcatctgg
    14101 acccacccgt tcgcgctcaa gaacccggca ccgtcggtct tcgtcgataa cttcggcgac
    14161 gacggcgtca acctgacggc ctacatctgg gcgccgaccc ggaactggtg ggacgtcagg
    14221 acaagcctcc tctggcagat caagcagaaa ctcgaggaga acggcatcga gatgcccttc
    14281 ccgcagagga ccgtccggtt tgcggatgac ctccgggtga cggccggcga tgccgggaaa
    14341 ggcggggcag aagactgacc ccgggttcag ggaactgcgg ggatgcggcc cccgtggtat
    14401 aaaggctgcg aaggagcgct tcaggtaccc gggatcgggc ttcgcgcgaa ctcgagggta
    14461 tgagcggtga aacggcctca cgcaaaagag cgcaaagggg cgttatgtgc ccctatactc
    14521 cgtaaatggg taaattctct gcagggtgcc gtgcacccct ctctcacacc cggtgcacct
    14581 tccccacccc gccgggccgg aaggtccccg gatacctctc ccggatctcg gggatcgcat
    14641 cggtgcagtg cgatgccgag agatactcca caccggcgag cgcgccgata tccccggtgg
    14701 tgacggtatg gaatccgccg acgacccccc ataccctgcc ctcctgagag acccggtcga
    14761 cgatccggcc gatgtgggga tgcgcgcacc cggtgatgac cagatagccg ccgggggcgg
    14821 tgattgcaag cgactgctcc cgtatatcgg tcccgagcgg gccggtgacg gcgataccgt
    14881 ccgcgatctc catccactcc cggacgatcc ggggttttgt gtactccccg atcatcccga
    14941 gcgtctgcgc ggtgaaggcg tcgtgaacga agacctccat cgaggggttt acgccgagga
    15001 tcgcgcgaac ccctccggta tgatcccagt ggtcgtggga gaggacgagg tacctgatat
    15061 ccgcagggtc gatgccgagc gcccgcatgt tggatagcag tacgtcgcct ttcgccccgg
    15121 tgtcaaagag cagccccgcc tcctcgacca ggcaggagaa cccccagtcc ggcgtgagac
    15181 cctcccggca ggggatgttg ttgtagacct cggtgatggt gaacatgatg aaaactccat
    15241 ctcccggtct ctcttcccaa acgattatgc tacccgatgc ggcagacccg gggggtatcg
    15301 ggcccgggag ttcggagcac ccccgggcct gcagacgaaa aagagggtta cgctatgatg
    15361 taggcgatgg agaccgtcgc ggtcacgtcg atctcgccga tctcgaccgg ggtcggggcg
    15421 ctggcgccct ttgccatatc agtagcggcc aggtagcggt tatcgtaggc catggggatg
    15481 tagttgctcc cgacattgac ctccttgaca tcgacgacgg tcttgccgag ggcggcgacg
    15541 accgcatccg cgtcgcttcg cgcctgtttc acggcggcgg tcagggcctc cgaccggaac
    15601 tgctgctgtt tttcatcgct caaggtgagc atgagccggt caacccggtt tgccccgttc
    15661 gcgaccgcga tatcgacgat ctcgcctgca cgatcgacgt cgtgtaaggt gatctcgagg
    15721 ttgttggtca cccgatagaa tttaacccgg gaggtcgcca ggggtttgtc gtctatctcg
    15781 gtcaccggca tgatgttgta gccggcggtc ctgatatcct ttgccgggat gccggcgttc
    15841 ttcagggcgt tcgtgacggc atccatcttc tgggcgttct gctgttgcgc gactttcgca
    15901 tcggcgttct cgatctcgac cgcgagcacg atgatggcct ggtcgggcgt ggtcgttact
    15961 ttgccggtgc cggatacgtg gatcagtttg tccccggatt cctccggcgt ctctgcggtg
    16021 acgttcccga tcaccgcggc gcagaggacc atcagggcga tccccaggag tgctgccttt
    16081 cctcgcatgg tatcgatcat ctcttgattt gacgaaaata aatatcttgc tttaactacg
    16141 ggaattttcg tagttttaag aggaccgatc ccggaaatgt ccgtttttat ctcccggagg
    16201 tgagaggatg gagaagcggg aagacccaag cccggaaggc gcagggatga gagcggggcc
    16261 ggctcatacc cgcaggatac cgcataccct cccgtcccgg cagatcctgc aaaactcgac
    16321 ggttacagcc acccgcacgc ccgtgcgggt gccccggaag aagagagcct caaggcgggg
    16381 aatggtccgg tgcccgggta ccgcatgccc ggtacgggag accggctctc aggataaata
    16441 cagaacggca tcagacccct gcttcatcac acggatcagt gggggtaact ctcatcatct
    16501 gccatcacat ggtcggcgta tgacctgtta atgcttcggc gccccgggtg ctgcagtgct
    16561 ggaaccgctc cgtgctccac ccggagtccg gaggctcaca gggctgccgg aagcgccggg
    16621 agcggcctat ccggggcggc aatcgaggca aaggtatata tcgctgcaat gcctgtctgg
    16681 agctcgatgc ttcgaggggg aggggagtgt tccgggggat ccgcacacgg cggccctgcc
    16741 cggagagaac cgcgatgaaa gcgggtttca ggcgcggccc ctccctttcc gggagagagg
    16801 tatgacgccg gtgcggctgc gggctgtcgg ggatgtgctg ctctggacca ggaacgggaa
    16861 aagaccgttt gacctggtac accatgagct ccaggagaag gacctgctct ttgggaacct
    16921 ggagacggtc ctctccgaga gcggcgatcc gagccggaag cgctgggtca acacaacccc
    16981 gccggaggcg gccgaatacc tcgaagaggc cgggttcgat atactgagcc tcgcgaacaa
    17041 ccatacgctc gatatggggc gggaggggtt tgaaaagacc ctcgacgtgc ttgagagcca
    17101 cggtatcgcc tacatcggcg gcgcacgggg cgacgggccg ccgcaggccc tggtcgtcga
    17161 gaggaggggg atcaaactcg gattcctggc gtatacgaac ggcatgttcc ggtcgccgcc
    17221 gggcgtcgcc gtatccaggc tgaagaaaaa gaccgtcatc gacgagatca ggaggctgaa
    17281 ggaccgctgc gaccacatca tcgtctccct gcactggggg atcgagaacg tctactaccc
    17341 ctctccggcc caggtcaggc ttgcccacga cctcatcgac ggcggagcaa ccctgatact
    17401 cgggcaccac tcgcacacca tccaggggct ggaacggtat aaggacggcc tgatcgccta
    17461 ctccctcggg aacttccagt tcgataccgg actcttcaaa gagaagatca agagcaccat
    17521 gatcctctcg gtagaccttg accggggcgg tatccgggac tatacggtca tcccctgctt
    17581 catcaacgac gatttccagc cggaggcggc ggaggggcgg ataggggaga aggtagagcg
    17641 ctggatcacg gagtgttcgg agagcgtcca gaacggcagg gtcacccgga ggtggtggtt
    17701 tgaggagatc ggcgaggagt acatcaccta taacctggag agttaccgct accgcatcag
    17761 gcagcacggc ctcgcccccc tggtcgagtg cggggtctgg ttggtgaccc cgttctgcct
    17821 gaactgttat gcaggcgtga tcaggagacg gttgagagaa cgatatacag agggatatgc
    17881 gtgaatatat tgcaggttac accgtttttt aaaccggtct gggagtcggg gggagtggcg
    17941 cgggtcgcat acgacatatc gcggaacctt cacgagaacg ggcacgccgt cacggtctac
    18001 acgacgaacc ggagcatcta cccaagcgac ctgccgacaa accgcccgac cgatgtcgac
    18061 ggtatgaacg tctactactt cgagaacctg cgcaggtata tccccggcgc gacaccgccg
    18121 gtgataccct atcgcatgcc ggcggtcgca aagagagaga cggcacgatt cgatgttatc
    18181 cacatccacg accaccgcac cctcctcacc gtcatcgcct cgcactacgc gcgaaagtac
    18241 ggcgtgccgt acgtgctcca ggcgcacggg gcgctccccg acgacaccgg ttctgcgcga
    18301 atgaaacggt tgttcgacgt tctctgggca aagaaggtga tcctcggcgc cgcaggagtg
    18361 atcgcgctca acgagacgga ggcagagcgc taccgcaacc tcggcgtcga tgaggagaag
    18421 atcgccatcg tcccgaacgg catcgatctt gctgaatacc ccgacctccc tccccgcggc
    18481 aggttccgcg cagcatgggg tatcgatgac gccacgaagg tcgtgctcta cctcgggcga
    18541 ctcgacccga caaaagggat cgatctcctg atccgctcgt ttggagaggt cgggcaggag
    18601 ttcggcgacg ccgttctcat gctggtaggc ggagatatgg gccacaacga ggagttcagg
    18661 cggcgggtcc ggtcgcttgg ccttgacgac cgggtgatct tcacggggtt cgtgagcagg
    18721 gaggacaaga tggccgcgtt caccgacgcc gacgtcttcg tgacgccgag cttcaccggc
    18781 tttcccatca cgttcctcga ggcatgcctc tgcggaacgc ccatcgtgac gacggggaac
    18841 ggggacctcc tcggatgggt cgacaacacc gtcgggttca acaccgcata tacaccggag
    18901 gcgcttgccg gcgccatcgg gcgcctgctc gctgacgacg ccctgcgaga cgggtttagc
    18961 aggcaggcga aggagatcgt ccggacgcgc tacaactggc aggcgatcgt ccatgagatc
    19021 gaggccctgt atgcgaccgt agcaaacggc tacagggagc cgcggggcgg ataagcgttc
    19081 gccggcgggg atccggccgc ccgggaagca caaaagaggt acgggagccc gcaggcgcgt
    19141 tctttaaaaa aaggttgagg acgaggtatc ggtttactcg ccggcaggca taactttcat
    19201 gcccgaggtc cggacgttcc cgccgccctc gaccaggaac gccctggcgg agccggatcg
    19261 gatctcgccc gagtactcga cgttctggtt gttctttgcc cagaccagcg tctcgacccg
    19321 gtcgccggag gcgacgatat cgaccgacga atcgccgaac tggccgttcg tgccgccgag
    19381 gcttgcacct ttgccgttga tcccggccgg gttctccagg tggaagtttt cgatccggac
    19441 gttcttcgcg aggtcgacct ggaggccgta acagttcgcg ttgatgctcg tgcagttct
//
LOCUS       DAOSII010000021        19480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_3058_length_19480_cov_46.856371, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000021 DAOSII010000000
VERSION     DAOSII010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 19480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19480
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3058_length_19480_cov_46.856371"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="PLV90_02915"
     CDS             <1..1165
                     /locus_tag="PLV90_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HOF96098.1"
                     /translation="GRRLSIIAASVVFLAGTGIVVGATDLSIFLVGRVLIGIAIGVAS
                     FVSPLYISEIAPPAFRGGMVSLNQLFITFGILISYGVDFLFSSSGDWRAMFALGAVPA
                     AVLLIGMVLLPDSPRWLLSRQHMNRAIAVLEKARGTGDVFGELDDIKKAIGMQKGGNW
                     SDLLASSIRMPLIVGLGLAVLQQLTGINTVIYYAPTIFQFSGLHSVGASIAATAGVGT
                     VNVIATAVAVVLIDRVGRRPLLLVGVAGMVVSLAVLGAGFAFSGTVQGGSLLGLITAV
                     SLMTYIASFAIGLGPVFWLLISEIYPLTVRGRAMGVTTIANWAANFLITVTFLTLVGA
                     IGQAVVFWLYALVSIIAWFFVFRLVPETKGLTLEEIEEHFRAGRHPRELKGLP"
     gene            1179..3065
                     /locus_tag="PLV90_02920"
     CDS             1179..3065
                     /locus_tag="PLV90_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HOF96099.1"
                     /translation="MSNQIQQNRPQAHGEYRPIEDYGVIGNGHTVALISSSGSIDWLC
                     FHRFDSPSLFARILDPGRGGYWSVTTKGAFESSHRYQEETNILETTLRCAGGTVVLRD
                     FMDIASIARLRRLPAPGRIVRIAECTDGKVGVVSRCQPRPNYARTHPEFALYGDQVTF
                     GGYTLTGPTAWQVDDADASLTCRITLRAGETTAFTLATEDDATLPQRTPADALKVTTD
                     YWRKWSSACTYRGPYRDIVIRNALTLKMMTYEPSGAIVAAPTTSLPETFGGERNWDYR
                     FTWIRDASLTLYALLLAGYLDNEQPFFDWLVRTVKLKGTGISILYPIIPEGRIAETVL
                     DNFRGYRDSRPVRIGNQAAVQEQLDVYGEVMGAIQFAWRIGKYDPTPLWGTMRQMLDW
                     VTRHWHDRDSGLWEVRGGVRHFVYSKAMMWFALDCGIEIAEGMRLSGDIKTWRRERDL
                     IHEEVLDKGWSDDLGAFKQSYEDENLDAANLRLPLINFVGGDDPRMLSTIDATLEHLV
                     VDGLCYRYIDAPEGVTGKEGSFVVCTTWLINALIRAGRTGEAHRMFQNLLARASPLGL
                     YAEEIQPATGAHMGNFPQSFSQIGIINAAVSLAHAGQVGTVSPHHAAAADAAGHGGGG
                     KRTG"
     gene            3151..3870
                     /locus_tag="PLV90_02925"
     CDS             3151..3870
                     /locus_tag="PLV90_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOF96100.1"
                     /translation="MKERPGLRYAAASDIGGRQQNEDAFFADEAAGYSVFAVADGLGG
                     HARGDVASKMAIRALEETARECLPGLKPARVLELAFNRANARIYTYNQENRLNAGTTL
                     SAAIVDGSGGCWIGTVGDSRTHVIRSSSVWHTRDQSYVQGLVDAGVLSPVEAMLHPKK
                     NILTGALGLSAGVQVDLDAVDAAGAVLVLSSDGLHDCVPADAIRDIVLADEPDAACRR
                     LIDAAKDAGSTDNITVIVARG"
     gene            complement(3877..4392)
                     /locus_tag="PLV90_02930"
     CDS             complement(3877..4392)
                     /locus_tag="PLV90_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOF96101.1"
                     /translation="MTHAMLPDTGDEHPRDLQPLLIRLFDPDKAVRAEAVHGLIAIGS
                     PAVPACIALLQDADWRVRYRAAEVLGRIGDADAFAPLVAALGDEKDHVRYMAAKGLGL
                     LCDPRAVAHLGVMQRDENEFVRRSAAGSLGRIGGAEAVGLLHAALGDEATEGVRAAIL
                     AALRDAGAEGT"
     gene            complement(4565..4690)
                     /locus_tag="PLV90_02935"
     CDS             complement(4565..4690)
                     /locus_tag="PLV90_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96102.1"
                     /translation="MHVLVKWIESDPVFKTLGPLIVGIGILLLVSLILYALGYLI"
     gene            complement(4905..4977)
                     /locus_tag="PLV90_02940"
     tRNA            complement(4905..4977)
                     /locus_tag="PLV90_02940"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4941..4943),aa:Asp,seq:gtc)
     gene            complement(4994..5100)
                     /locus_tag="PLV90_02945"
     tRNA            complement(join(4994..5029,5063..5100))
                     /locus_tag="PLV90_02945"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5064..5066),aa:Tyr,seq:gta)
     gene            5248..6792
                     /locus_tag="PLV90_02950"
     CDS             5248..6792
                     /locus_tag="PLV90_02950"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOF96103.1"
                     /translation="MKRTVFFTDSRAKKNVTVFDTTLRDGEQTPGISFTREEKFGIAE
                     HLSNIGVHTIEAGFPASSAAEHEIVRAIKGLGLSAQVCGLARSLPADVDACIDCDVDM
                     VHVFIPTSDVQREYTIKKTREQVLAATGDIIAYVRDHIGRCMFSAMDATRTDWDYLME
                     VYRVAVDAGATIINVPDTVGVITPTAMKHLITRIDREVDCPIDVHCHNDFGLAVANTI
                     AAVEGGASQVQVTVNGIGERAGNADLAQTVMILSSIYGVETGIRTTSLVETSRLVARY
                     AGMSIPAIQPVVGENAFAHESGIHSHGVITRSDTFEPGIMTPEMVGHRRRLKLGKHAG
                     RHAVRQMLAEVHMTPTDAQLDEIILRVKAIAGRGKRVTDADLYEIGESVMQIVSNEKT
                     LELQDIAIMTGNHVIPTASVRATVDGVEHAFSSIGNGPVDAAVRAILGIIPAPVHLKE
                     FNIEAISGGTDALGHVTITVEDERGRVFDASASSDDIILASVEAVINAINLVCRTQKN
                     DKPRKE"
     gene            complement(6817..7182)
                     /locus_tag="PLV90_02955"
     CDS             complement(6817..7182)
                     /locus_tag="PLV90_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit beta"
                     /protein_id="HOF96104.1"
                     /translation="MENIPPKVQNQLAMLQQMQQQLQTVVSQKAQYELTIREARRAVE
                     ELGDVPEDAAVFMSVGGVMMQKGREQVLTSLNERVETLELRVKSLEKQEKALQGRFEQ
                     LSSQIRGALEGKQQPPGAS"
     gene            complement(7454..7690)
                     /locus_tag="PLV90_02960"
     CDS             complement(7454..7690)
                     /locus_tag="PLV90_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HOF96105.1"
                     /translation="MHTAVFTFATDDARALYRSVCQEMGDVGDRSSVRVRLADDNTLL
                     LEATAADIPALRAALNTWLRLINIAIEMHEIVAP"
     gene            complement(7690..8094)
                     /locus_tag="PLV90_02965"
     CDS             complement(7690..8094)
                     /locus_tag="PLV90_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96106.1"
                     /translation="MTVVTTSRKPLPELRSLARDLAFAIGAGYTTRGKAGMGDLFALD
                     ESVVIVSRSGPFFLIQVFVDGETITEITVRSFAVEERPGEIARGLFVSDRPVYEALKD
                     RFNVAFAGETLPRRRIVFDGTQRKRYTLEVLP"
     gene            complement(8091..8222)
                     /locus_tag="PLV90_02970"
     CDS             complement(8091..8222)
                     /locus_tag="PLV90_02970"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit P"
                     /protein_id="HOF96107.1"
                     /translation="MAAYKCARCKQKVEIDVNIRCPYCGYRILFKERGAGIKDLKAR"
     gene            complement(8229..8519)
                     /locus_tag="PLV90_02975"
     CDS             complement(8229..8519)
                     /locus_tag="PLV90_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37ae"
                     /protein_id="HOF96108.1"
                     /translation="MAKRKQSAKGRVVGSSGRFGPRYGRFIRKRINQLEQVSRANHAC
                     PRCDQVAVRREGTGIWACRKCGFKFAGGSYVPETPVMRVAARSIERSLQREG"
     gene            complement(8575..9279)
                     /locus_tag="PLV90_02980"
     CDS             complement(8575..9279)
                     /locus_tag="PLV90_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly factor SBDS"
                     /protein_id="HOF96109.1"
                     /translation="MIPLDQAVVARLESHGERFEVLVDPDQAMRIRRGEEIEIEEAVA
                     ANYIFENAAHAERASDEALLKVFKTTEFEPAALRIIQKGEIHLTSEQRRHLIVEKRNR
                     IITFIARNAVNPQTGFPHPPQRIELAMEEARVNVDPFKSVEEQVKETVKALRPLLPIR
                     FEEIRIAVKIPADYAARAYGELQSAVTLEQNEWQKDGSWIAVVRIPAGAQEELYDLVN
                     RISRGNAETRIIERRS"
     gene            complement(9294..10016)
                     /gene="psmA"
                     /locus_tag="PLV90_02985"
     CDS             complement(9294..10016)
                     /gene="psmA"
                     /locus_tag="PLV90_02985"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit alpha"
                     /protein_id="HOF96110.1"
                     /translation="MQPQYQMGYDRAITVFSPDGRLYQVEYAREAVKRGTTAVGIKCR
                     EGVVLIVDKRVTSRLLEPVSIEKIFKIDTHIGVASSGLVGDARSLVDRARVESQINRV
                     SYSEPINVEILAKKLCDHMQTYTQFGGARPYGTALLIAGVSDGEARLFETDPSGTLLE
                     YKATGIGTGRPAVMKVFEDEYQEDADFSGAIKLGIKALHAATEGKLDVSAIEIGIVSI
                     ATREFKRLDKDEVKVYVDQFEE"
     gene            complement(10016..10498)
                     /locus_tag="PLV90_02990"
     CDS             complement(10016..10498)
                     /locus_tag="PLV90_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpp14/Pop5 family protein"
                     /protein_id="HOF96111.1"
                     /translation="MRPRPPAMRTKRRYILARILPYGATPDQKDVYLSVVEAATSLFG
                     DAAVGLAQPAVVFCDGGYAVIRCRRGTEKDIATALATVTAIGVCRAALRAVAVSGTIH
                     ALKRRVRPIRPLSGDGEMTIEGRCFTMYRYPRQKVDLVEKSIKHQKLLFFTEPDLEER
                     "
     gene            complement(10495..11139)
                     /locus_tag="PLV90_02995"
     CDS             complement(10495..11139)
                     /locus_tag="PLV90_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P subunit p30 family protein"
                     /protein_id="HOF96112.1"
                     /translation="MKITDASVRPYPAGDSTLARMAVEAAELGFDSVVAIGEGAPPVG
                     GAAVLRGAVISGASPKEVIKQVREPAAQHADVVFVNAGDIAFNRAVVSIKEVQVLRNI
                     HAARKNAFDHVAARSAAQRGLAVDISLAPLIQYRGTKRQRALQQYEDILLLHRRYGFL
                     LTISSGACSILEQRSVREIRGLCALFGMTGAEVNEALASVGRIVEPDQPVRVVG"
     gene            complement(11172..11762)
                     /locus_tag="PLV90_03000"
     CDS             complement(11172..11762)
                     /locus_tag="PLV90_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15e"
                     /protein_id="HOF96113.1"
                     /translation="MAKSMYAYVREAWKRPDRSEVKSLLWERLQQWRREGSIVRVERP
                     TRIDRARSLGYKAKQGIIVVRVKVRRGGRRKPRYIRGRRTARMGMRRMTPAKSLQRMA
                     EERASRKFPNMEALNSYWVGEDGRSKWFEVILVDGHHPSIRSDRNLAWLADPTHRGRA
                     ERGKTSAGMKGRGMRTRGRGTEKTRPSIRSHANRGK"
     gene            complement(11880..12374)
                     /gene="moaC"
                     /locus_tag="PLV90_03005"
     CDS             complement(11880..12374)
                     /gene="moaC"
                     /locus_tag="PLV90_03005"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOF96114.1"
                     /translation="MNESGRHGRTPVFTHIEDDRAQMVDITAKAEVPREAVASGRIYL
                     REETLRAIREGTTIKGNVLATARVAATLAVKDTPRIIPMCHPIPLGGITIDFAEGDGY
                     IEATARVKSYGRTGVEMEALTGVSVALLTVWDMVKSAEKDENGQYPVTRIDAIRVVEK
                     RKGL"
     gene            complement(12432..13823)
                     /locus_tag="PLV90_03010"
     CDS             complement(12432..13823)
                     /locus_tag="PLV90_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOF96115.1"
                     /translation="MATGELREFRETGVIDAGRMRAIEENAVALGYPSLLMMESAGRA
                     VADAVLASDPDRILILCGRGNNGGDGMVAARYLQHIGAVDVVYPECGSMTPSAAAQAS
                     LLRHCTAALHPVRCATDVKPLGRLFEEADVIVDAMLGTGASGAVREPLATLVAGANAS
                     PAPIVAVDIPTPGIRANRILSFHRPKVEGADVADIGIPLDAEIYTGPGDLLLVPARSP
                     GAHKGAGGEVLVIGGGPYQGAPYIAAMGALRAGADIVRIASPAYIPMPDLIYERLEGK
                     VITEEHLATILSLVDRTDVVVCGMGLGKESHDVVLAIAEAAERAVFDADALARPLPAA
                     KRTIYTPHAGEFARMTGREPPADLAARARCVRGARTTGTVLLKGPVDVISDGARVRFN
                     RTGTPAMTVGGTGDLLAGIAGALFCRLPAFEAAGIAAYVNGRAGMRAAEGRGCGMLAT
                     DMLDFIPEELFPV"
     gene            13957..15354
                     /locus_tag="PLV90_03015"
     CDS             13957..15354
                     /locus_tag="PLV90_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOF96116.1"
                     /translation="MGLERDVQRAVDRISEADSVTIISHIDADGISTESILAQALARE
                     GIPATSVFVRQLEPMAMRHIPDDDSLKIFTDLGAGQQALLEEHGLSADGVLIIDHHVS
                     QPCGTAYPQVNCLDFGITRMSAAGIAYMVARAIDSANTDLAKLAVVGNVGDMMARENC
                     GLVGPAREIVQDGVEYGNVIVQRADLNCYGISTRPVHVCLGRCDDPYIDGISNSVTRA
                     LRFLERLGVTLKTPRGGWLVWEEVPLDDRRTIISALTQQLVAHGREADRLLAETYIFP
                     DEPERTPFRNASEYATLLNACGRWAKPKVGGRICRGDRGDAYREAEYMLAHHRSVIRG
                     LLQYILETGVTELSHLQYIHTGDRFPDTIVGIGAGMALSKLNWKKPIMVLAAMVDEPD
                     VTKVSMRTNEWALARGVDLQAALVEASATVGGAGGGHRIAAGAFIPHDTEEEFIDGIN
                     RILTRQFASADTDNS"
     gene            15296..15796
                     /locus_tag="PLV90_03020"
     CDS             15296..15796
                     /locus_tag="PLV90_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOF96117.1"
                     /translation="MVSTEYSRDSSLRRIRTIADFQFGAGSGEALFPEGCTFEFSTTG
                     RIRQVRLGNERLVTVRAQDGRLTLGIAGAARLAAVLAPPTYRVTVREDVAAFIADGKN
                     AMAKHVVAADEGIRAGDEVLVVTGDDMLLATGAALLSGREILAFNYGVAVKVRQGRGS
                     ECFQAR"
     gene            15778..16119
                     /locus_tag="PLV90_03025"
     CDS             15778..16119
                     /locus_tag="PLV90_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nascent polypeptide-associated complex protein"
                     /protein_id="HOF96118.1"
                     /translation="MFPGKINPRKMKQMMKQMGMEMEELEGVEKVVIYTPAGNYVFDD
                     AQVVATTMQGVTTYQLTGEARFEEAVPEIPDEDVDLVASQAGVAGEVAKKALIETRGD
                     IAEAILKLTQQ"
     gene            16116..16841
                     /locus_tag="PLV90_03030"
     CDS             16116..16841
                     /locus_tag="PLV90_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HOF96119.1"
                     /translation="MIETGEQILLVGEDREYFVTAGEGQFSTDRGMIDRGALVGMSPG
                     DEIRTHLGAPFTVLCPRSTDFFVHAKRSGAPMLPKDIGTVIAYTGMNRDDTVLDAGTG
                     SGVAAIYFGKIVRAVKTYEVRPEFARLAEKNIRAARLDNVDVIAADMLTATGAFDVVH
                     LDLTITPAHVEHAFSLLSPGGYLATYTPFLEHTFVALDAATPLFRDVHCYECMERELT
                     RSARGTRPSTRVAHSGYITIARK"
     gene            complement(16845..17033)
                     /locus_tag="PLV90_03035"
     CDS             complement(16845..17033)
                     /locus_tag="PLV90_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96120.1"
                     /translation="MPEPEEEYVSEVCCHCGGLGCLYCNKKGTVMVHKPPQKCTHCGG
                     DCCIYCGYTGWERPLKEY"
     gene            17240..18136
                     /gene="pyrB"
                     /locus_tag="PLV90_03040"
     CDS             17240..18136
                     /gene="pyrB"
                     /locus_tag="PLV90_03040"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOF96121.1"
                     /translation="MYHIISIHDFERSDLDYLLDRAKEFDTGGYRSRALEDKLAALLF
                     FEPSTRTRMSFATAMARLGGRWISVDSVEASSIVKGETLADTIRVMSNYADAIVLRHP
                     KEGAARLASEFATVPVINAGDGAGQHPSQTLLDLYTIRQSMPIDGIDVGLLGDLRYGR
                     TAHSLALALAHYGVTLHTIAPGGLEMPASITLELREHGMEVVEHADVGEVIRDLDVLY
                     VTRIQRERFPDSASYYNVASSYRITLDLLNGVRDDLMILHPLPRAGEIDSAVDRTPYA
                     RYFEQAKNGVPVRMALLHEVMK"
     gene            18133..18600
                     /gene="pyrI"
                     /locus_tag="PLV90_03045"
     CDS             18133..18600
                     /gene="pyrI"
                     /locus_tag="PLV90_03045"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HOF96122.1"
                     /translation="MSRKDPSGGLLISPIRNGTVIDHITAGEALNVLRILGITGSTRE
                     CLSIATNVESKRLGRKDIVKIENRELRTEEVDRIALIAPHAKINIIRDLQVVEKKGVE
                     IPRVLRGVVRCPNPGCITNTNEPVESTFEVLDKGLHCLYCDWLIKNDIANHII"
     gene            complement(18593..19051)
                     /locus_tag="PLV90_03050"
     CDS             complement(18593..19051)
                     /locus_tag="PLV90_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine adenylyltransferase"
                     /protein_id="HOF96123.1"
                     /translation="MRVMVGGTFDPLHAGHRKLLSRSFELAGPDGEVVIGLTTDEFAG
                     AKVHPVRSYRERLASVASFIREHGYTAAWRVEPLSDRYGSALNADFDILVVSEETFPV
                     AVEINDLRRNRGKKRVDLHEISCVLAEDGRRISSTRICRGEIDRHGRLIR"
BASE COUNT         3544 a         6129 c         6141 g         3666 t
ORIGIN      
        1 cgggcggcgc ctctcgatca tcgctgcgtc ggtcgtcttc cttgcaggca ccgggatcgt
       61 cgtcggtgca acggacctct ccatcttcct ggtcggccgc gttctgatcg gcatagccat
      121 cggtgtcgca tcgttcgtca gccctctcta catatcggag attgcgcctc cggcgttccg
      181 cggcggcatg gtgtcgctca accagctctt catcacgttc gggatcctca tctcctacgg
      241 ggttgacttc ctcttctctt caagcggcga ctggcgggcc atgtttgccc tcggcgcggt
      301 gccggccgcg gttctcctca tcggcatggt tctgctcccg gatagcccca ggtggctttt
      361 gtcccgacag catatgaacc gggcaatcgc cgtcctcgaa aaagcccgcg ggaccggtga
      421 tgtctttggg gagcttgatg atatcaagaa ggcgatcggg atgcagaagg gcggcaactg
      481 gtcggacctg ctggcttcat cgatacgaat gcccctgatc gtcggtctcg gcctggccgt
      541 cctgcagcag ctgaccggca tcaacaccgt catctattac gccccgacaa tctttcagtt
      601 ttcagggttg cactctgtcg gcgcttcgat tgcagcgact gcgggcgtgg gcaccgtgaa
      661 cgtcattgcc accgccgttg ccgtcgtgct catcgatcgg gttggacgcc gccctcttct
      721 cctcgtgggg gtcgccggca tggtcgtgag ccttgccgtg ctcggtgccg ggtttgcttt
      781 cagcggaacg gtgcagggcg ggagtctcct cgggctgatc accgcggtca gcctgatgac
      841 gtacatcgct tcgtttgcta tcggcctcgg gccggtgttc tggctcctga tatccgagat
      901 ctacccgctc accgtgcggg ggcgtgccat gggcgtcaca accatcgcca actgggccgc
      961 gaacttcctc atcaccgtga cattcctgac gctggtcgga gcaataggac aggccgtcgt
     1021 cttctggctt tatgcgctcg tgagcatcat cgcgtggttc ttcgtcttca ggctggtgcc
     1081 cgagacgaaa ggactcacgc tcgaagagat tgaagaacac ttcagggccg gcaggcaccc
     1141 ccgcgagctg aagggtttac cgtgatcacg tgatcgatat gtccaaccag atccagcaga
     1201 accggccgca ggcccatggt gagtaccggc cgatcgagga ctacggtgtg atcgggaacg
     1261 ggcacaccgt ggcgctcatc agcagcagtg ggtccatcga ctggctctgc tttcacaggt
     1321 tcgactctcc atcgctcttt gcccggatac tcgatcccgg ccgcggcgga tactggagcg
     1381 tgacgacgaa aggagcgttc gagagctcgc accggtatca ggaggagaca aacatcctcg
     1441 agacgaccct caggtgtgcc gggggaaccg tggtcctccg ggacttcatg gatatcgcaa
     1501 gcattgcccg cctccgccgg ctcccggccc ccggcaggat cgtacgcata gcggaatgca
     1561 ccgatgggaa ggtgggggtc gtctcccgct gccagccccg gccgaactac gcccgcacac
     1621 accctgagtt tgcgctttat ggagatcagg taacgttcgg tgggtatacg ctcaccgggc
     1681 cgacggcatg gcaggtcgac gacgcagatg catcgttgac ctgcaggatc acgctccgtg
     1741 cgggcgagac gacggcgttc acgctcgcaa cggaggacga cgccaccctg ccgcagcgta
     1801 cgcctgcgga tgccctgaag gtgacgaccg attactggcg aaaatggagc agcgcctgca
     1861 cgtaccgggg gccgtatcgc gatatcgtca tccgcaacgc cctcactctc aagatgatga
     1921 cctacgagcc gtccggggcc atcgttgccg cgccgacgac atcgctcccc gagaccttcg
     1981 gcggcgagcg gaactgggat tatcgcttca cctggatacg cgatgcgtcg ctcacgctgt
     2041 atgccctcct ccttgccggc tacctcgaca acgagcagcc gttcttcgac tggctcgtgc
     2101 gcacggtcaa actgaaggga acgggcatat cgatcctcta tccgatcata ccggagggca
     2161 ggatcgccga gacggtgctc gataacttca ggggataccg ggattcccgg ccggtgagga
     2221 ttggaaacca ggcagcggtc caggagcagc tcgacgtcta cggcgaggtc atgggggcca
     2281 tccagttcgc ctggcggatc gggaagtacg acccgacgcc gctctggggg acgatgcggc
     2341 agatgctcga ctgggtgacc cgtcactggc atgaccgcga tagcggactc tgggaggtca
     2401 ggggcggcgt gcggcatttt gtctacagta aagccatgat gtggttcgct cttgactgcg
     2461 ggatagagat cgcagagggt atgcgccttt ctggggatat caagacctgg cgccgcgagc
     2521 gcgacctgat ccacgaggaa gtgctcgata aggggtggag cgatgatctg ggtgcgttca
     2581 agcagtcgta cgaggatgag aacctcgatg ccgcgaacct gcgcctcccc ctcatcaact
     2641 tcgtcggggg ggatgatccg cggatgctct cgacgataga tgcgacgctc gagcaccttg
     2701 tcgtcgacgg actctgctac cgctacatcg atgctcctga gggcgtgacc gggaaggaag
     2761 ggtcgttcgt cgtctgcacg acctggctca tcaacgcgct gatacgtgcc gggcgcaccg
     2821 gagaggcgca ccggatgttt cagaaccttc ttgcacgtgc aagtcctctc ggcctctacg
     2881 ccgaagagat ccagccggcg accggggccc atatgggcaa cttcccgcag tcgttctcac
     2941 agatcggtat catcaacgcc gccgtctcgc ttgctcacgc agggcaggtc ggcacggtca
     3001 gcccgcacca tgccgccgct gcggatgcgg cgggacacgg cggcgggggc aaacgcaccg
     3061 ggtgaggcga agggtgacga cggtgaaggg agatggctcc ggggaccggg caccaaaagg
     3121 ctattcgtcc ggccatccag agtggtatac gtgaaggaga ggcctgggct ccggtacgca
     3181 gcggcgagcg atatcggcgg gcggcagcag aacgaggacg cgttctttgc cgatgaagcg
     3241 gccggatact ccgtcttcgc cgtcgcggac gggctcgggg ggcatgcccg cggggatgtt
     3301 gcgagcaaga tggcgatccg ggccctggag gagaccgcga gggagtgcct tcctggatta
     3361 aaacccgccc gggtgcttga actggcattc aatcgggcaa acgcccggat atatacgtat
     3421 aaccaggaga accgcctgaa cgccgggacg accctctcgg ccgcgatcgt cgatggctcc
     3481 ggggggtgct ggatcggcac cgtgggcgac agcaggacgc acgtcatccg atcatcgtcc
     3541 gtctggcata cgcgcgacca gagttacgtc cagggcctgg tggatgcagg ggtcctctcc
     3601 cccgtcgaag cgatgcttca tccgaagaag aacatactga ccggggcgct cggcctctcg
     3661 gccggggtgc aggtcgacct tgatgccgtg gatgccgccg gggcggtcct ggtgctcagc
     3721 tcggacgggc tccatgactg cgttccggcg gatgcgatcc gcgatatcgt gctcgccgac
     3781 gagcctgatg cggcatgccg gaggctgatc gatgccgcga aggatgcggg aagcaccgac
     3841 aacataacgg tgatcgtcgc gagagggtga cggggctcag gtgccctctg ctcccgcatc
     3901 gcggagcgcc gcaaggattg ccgcacggac gccttcggtc gcctcatccc cgagtgctgc
     3961 atggagcagg ccaacagctt ccgcgccgcc aatcctgccg agggagccgg cagcgctccg
     4021 ccgcacgaac tcgttctcgt cgcgctgcat cactccgagg tgtgcaaccg cccgggggtc
     4081 gcagagcagc ccgagcccct ttgccgccat gtagcggacg tggtctttct catcgccgag
     4141 cgcggcaacg aggggtgcga atgcatcggc gtcgccaatc cggccgagga cctccgccgc
     4201 acggtagcgc acccgccagt cggcatcctg cagcagggcg atgcaggcag gaacggcagg
     4261 gcttccaatc gctataagtc cgtgcacggc ttctgcccgc acagccttgt ccgggtcaaa
     4321 gagtcggata aggaggggtt gcaggtcgcg cgggtgttca tctccggtat ccggcagcat
     4381 ggcgtgggtc atatccagtc acatctgcgg gttgtagccg cttgcatcac atgttgaggc
     4441 ggcgttgtga taagagttga tcatttgcct ccggccggtg ccggggatac cgccgcacga
     4501 gagggtatct ggaggcgccg ggaaccacca tacggtgcag tatcccgact cccccggtgt
     4561 gccgtcagat cagatatccc agcgcatata gtatcaggct tacaagaagc aggatgccga
     4621 tcccgacgat gagcggcccc agagtcttga atacgggatc gctctcaatc catttcacca
     4681 gaacgtgcat ggcaccggat acaccattcg gatatatata ttttatctta aaaataaaaa
     4741 tcacatctga tatttaatgg tagtgcatcg cgccaggtac agtgccgggg aggttaacgg
     4801 ttatccggca actttcggca gttctcggtc tattccgggt ggcgagagga gttcccttgc
     4861 cggggatgcg ctctgctcgg accttgagaa aatgggagaa atgacgccca ggacggaatt
     4921 cgaatccgtg tcgacggcgt gacaggccgt catgataggc cactacacta cctgggcaca
     4981 tttgatgggt ggatcccgcc tcccggattc gaaccgggga catcgcggta gccgcgcagt
     5041 tcgcccgaga gcgaagacca tactacagcc gcgcactcta ccagtctgag ttaaggcggg
     5101 tttctgctct aacaatgttg tcgtgggttg ttattaaata tttcgtagaa tccccgggat
     5161 gttgcacgat ccggagctct ggctatatat acaacctctg ataatgagta agtatgaacc
     5221 ctacaaacgc tgaagcaccc aggaacgctg aagcgtactg tcttcttcac cgatagccgt
     5281 gcgaaaaaga acgtcactgt tttcgacacc acactgcgcg acggtgaaca aacgccgggc
     5341 atctcattca cgcgtgaaga gaagttcggt attgcagagc atctctcgaa cataggagtc
     5401 cacaccatcg aagcgggctt tcccgcatct tctgctgccg agcacgagat cgtcagggcc
     5461 atcaagggcc tcggtctctc cgcacaggta tgcggcctcg cgcggtcgct cccggccgac
     5521 gtcgatgcgt gcatcgactg cgacgtcgat atggtccacg tcttcatacc gacatccgac
     5581 gtccagcggg agtacaccat caaaaagacc cgcgagcagg tcctcgcggc caccggcgat
     5641 atcatcgcgt acgtccgcga ccacatcggc cggtgtatgt tctccgccat ggatgccacc
     5701 aggaccgact gggattacct gatggaggtc taccgcgtcg cggtggatgc gggagcgacg
     5761 atcatcaacg tccccgatac cgtcggcgtg atcaccccga cggccatgaa gcacctcatc
     5821 acccggatcg accgggaggt cgactgtccg atcgacgtcc actgccacaa cgacttcggg
     5881 cttgcggtgg caaacaccat cgcggcggtc gagggcggcg cctctcaggt gcaggtgacg
     5941 gtgaacggca tcggcgagcg ggcgggaaac gccgatctcg cgcagacggt gatgattctc
     6001 tcgtctattt acggtgtaga gaccggcatc cggacgacaa gcctggtcga gacctcgcgg
     6061 ctcgtcgcac gctacgccgg aatgagcatc cccgccatac aaccggtcgt cggcgagaac
     6121 gcctttgccc acgagagcgg tatccactcc cacggcgtga tcacgcggtc ggatacgttc
     6181 gagcccggta tcatgacgcc ggagatggtc ggccaccgcc gcaggctgaa actcggcaaa
     6241 cacgccggca ggcatgcggt ccggcagatg ctcgccgagg tgcacatgac gcccaccgac
     6301 gcgcaactcg acgagatcat cctgcgggtg aaggcgattg ccggcagggg caagcgggtg
     6361 acggatgcgg atctctatga gataggagag agcgtcatgc agatcgtgtc caacgaaaag
     6421 acactcgagc tccaggatat cgccatcatg accggcaacc acgtcatacc gacggcgagc
     6481 gtcagggcga ccgtcgatgg ggttgagcac gccttctcaa gcatcggcaa cgggccggtg
     6541 gatgcggccg tcagggcaat ccttggcatc atccctgccc cggttcacct aaaggagttc
     6601 aacatcgagg ctatatccgg tggcaccgat gccctcgggc acgtcaccat caccgtcgaa
     6661 gacgagcggg gccgggtgtt cgatgccagc gcctcaagcg acgacatcat cctcgcgtcg
     6721 gtcgaggcgg tgatcaacgc gatcaacctc gtctgccgga cgcagaagaa cgataaaccg
     6781 cggaaagagt gagttctcga taaaaaaaat tcgggttcag gaagcgccgg gaggctgctg
     6841 tttgccttcc agcgctcccc ggatctgcga ggagagctgc tcgaacctgc cctgcagggc
     6901 cttctcctgc ttctcaagcg acttgacccg gagttcgagc gtctcgaccc gctcgtttaa
     6961 ggaggtgagc acctgctccc tgcccttctg catcatgacg ccgccgacgc tcatgaagac
     7021 cgccgcatcc tcggggacat cgccgagctc ctcgaccgct cgcctggctt cgcggatagt
     7081 cagttcatac tgcgctttct gtgagaccac agtctgcagc tgctgctgca tctgctggag
     7141 catagccagc tggttctgga ctttcggggg gatattctcc atattcatca atcctccttc
     7201 gaatgggatg ggagtagcat gatctcgggg gcaggggcct ttgcggccct ctgtccattc
     7261 tctacatgat agggcgggga acccttaaaa ggtgagggtt cggaagagag aatattgtcc
     7321 cgtaacgagc cgcatggtac gctcgttcga ccgcccaggg gaggttgacg gttcggaaga
     7381 gagaatgctg tcttgtaacg aaccgcgggc ggtctatccc cggagggggt gccggcgacc
     7441 cgttccggca tcgtcagggg gcgacgatct cgtgcatctc gatcgctata ttgatcaacc
     7501 gcagccaggt attgagggcc gcacggagcg ccggtatatc ggcggcagtc gcctcgagaa
     7561 ggagcgtatt gtcgtccgcg agccgtacgc ggacggacga cctgtccccc acgtcgccca
     7621 tctcctggca gacggagcgg tagagggcac gcgcatcgtc ggtcgcgaac gtaaagaccg
     7681 ccgtatgcat cacgggagaa cctccagggt gtagcgcttc cgttgcgtcc cgtcaaagac
     7741 gatccggcgc cggggaagag tttcgccggc aaacgcgacg ttgaagcgat ccttgagcgc
     7801 ctcgtatacg gggcggtcgg agacgaaaag cccgcgggcg atctctccgg gccgctcctc
     7861 gacggcaaac gaccggacag tgatctccgt tatcgtctca ccatccacaa atacctgaat
     7921 gagaaaaaaa ggacctgatc gtgagacgat cacgaccgac tcatcgagag cgaagaggtc
     7981 gcccatgccc gccttgccac gggtggtata cccggcgccg atcgcaaacg ccagatcccg
     8041 ggcgagggag cgcaactcag gaagcggttt acgtgacgtc gtgacgaccg tcatcgggcc
     8101 ttcagatctt ttatgccggc cccgcgctct ttgaagagga tacggtatcc acagtaagga
     8161 cagcgtatat tgacgtcgat ctccactttc tgcttgcagc gagcgcactt ataggcggcc
     8221 acgaccggtt agccctccct ctgcagcgag cgctcgatgc tccgcgcggc gacgcgcatc
     8281 accggcgtct cggggacgta acttccgccc gcaaacttaa acccacactt gcggcatgcc
     8341 cagatcccgg taccctcgcg cctgactgct acctgatcgc agcgggggca cgcgtggttc
     8401 gcgcgggaga cctgctcgag ctggtttatt ctcttccgga taaaccggcc ataccgcgga
     8461 ccaaaccgtc ccgaacttcc gactaccctg ccctttgcac tctgtttacg ctttgccatt
     8521 tcgcgtacct cttatatgtc ctctaatttt tgtgctgtcg attaatatag ttactcacga
     8581 cctgcgctcg atgatccggg tctcggcatt cccccgggat atcctgttga ccaggtcgta
     8641 gagttcttcc tgggcacccg ccggaattct gacgacggcg atccaggaac cgtccttctg
     8701 ccactcgttc tgctcgagcg tcaccgcgct ctggagctcg ccgtaggccc gcgcggcata
     8761 atccgccggg atcttcacgg cgatgcggat ctcctcaaac cggatgggga ggaggggcct
     8821 gagcgctttg accgtctctt tgacctgctc ctcgacggac ttgaagggat cgacgttcac
     8881 ccgtgcctcc tccatggcaa gctcgatccg ctgcggagga tgggggaagc cggtctgggg
     8941 gttgaccgcg ttcctcgcaa tgaacgtgat gatccggttc cgcttctcga cgatgaggtg
     9001 gcggcgctgc tccgacgtga ggtggatctc gcctttctgg atgatccgga gtgccgccgg
     9061 ttcgaactcg gtcgtcttga agactttcag gagggcctcg tcggaggccc gttcggcatg
     9121 ggcggcattc tcgaagatat agttggccgc gacggcctcc tcgatctcga tctcttcccc
     9181 ccgccggatg cgcatggcct ggtcggggtc gacaagcact tcaaaccgct ctccgtggct
     9241 ctcaagccgc gctacaactg cctggtccag gggtatcata ggaggtgccc gctctactct
     9301 tcgaactgat cgacgtagac cttcacttca tctttatcga gtcgcttgaa ctcccgggtc
     9361 gcgatggaga cgataccgat ctcgatggcg ctcacatcca gtttgccttc cgtcgcggcg
     9421 tgcagggcct tgatgccgag cttgattgcg ccggaaaagt cggcgtcctc ctggtactcg
     9481 tcctcaaaga ccttcatgac cgcaggccgg cccgtgccta tcccggttgc cttgtactcg
     9541 agcagcgtgc cgctcggatc ggtctcaaag agccgggcct ccccgtcgct gaccccggca
     9601 atcaggaggg ctgtcccgta ggggcgtgca ccgccgaact gggtgtaggt ctgcatatgg
     9661 tcgcagagct tctttgccag gatctcgacg ttgatcggct cgctgtagga gacacggttg
     9721 atctgcgact cgacccgcgc tctgtccacg agagaccgcg catcgccgac gaggccggag
     9781 gatgccacgc cgatatgcgt gtcgatcttg aagatcttct cgatggagac cggctcgagg
     9841 aggcgggacg tcaccctttt atccacgatc agcacgactc cttcgcggca cttgatgccg
     9901 acggccgtcg ttccccgttt cactgcttct cttgcgtact caacctggta gagccttccg
     9961 tccgggctga ataccgtgat cgcccggtca tatcccatct gatattgtgg ttgcattatc
    10021 gttcctccaa atcgggttcg gtgaaaaaca atagtttctg atgcttaata cttttttcaa
    10081 ccaaatcaac cttttgacgt ggatagcggt acatggtaaa acatctccct tcaatcgtca
    10141 tctccccgtc cccggaaaga gggcggatcg gtctcacccg gcgcttgagg gcgtgaatgg
    10201 tcccggagac cgcgaccgcc cggagggccg cccggcaaac gccgatcgcg gtgaccgtcg
    10261 caagggcggt ggcgatatcc ttctccgttc ccctccggca ccggataacg gcgtatcctc
    10321 cgtcgcagaa gaccaccgcc ggctgtgcga ggccgacggc tgcatcgcca aagagcgatg
    10381 tcgcggcctc gacgaccgag aggtagacat ccttctggtc cggggtcgcc ccatagggga
    10441 ggatccgcgc caggatgtag cgccgctttg tccgcatcgc cggcggtctt ggtctcatcc
    10501 taccacccgc acgggctgat cgggttcgac gatgcggccg acggatgcga gagcctcgtt
    10561 cacttccgct ccggtcatgc cgaagagcgc gcagaggccc cggatctcgc gaaccgaccg
    10621 ctgttcgagg atcgagcatg cgcctgagga tatcgtcagc aaaaagccgt agcgccggtg
    10681 cagcaggagg atatcctcgt actgctgcag cgccctctgg cgcttcgtcc cccggtactg
    10741 gatgaggggg gccagcgata tatccacagc aagaccgcgc tgtgccgccg accgtgcggc
    10801 gacatggtcg aaggcgttct tccgggcggc gtggatgttc ctgagaacct gcacctcttt
    10861 gatcgagacg acggcacggt tgaaggcgat atctcctgca ttgacaaaga ccacgtcggc
    10921 gtgctgggcg gcaggctcgc gcacctgctt gatgacctct tttggggatg cgccgctgat
    10981 cacggcaccg cggagaaccg ccgccccccc gaccggggga gcaccctcgc cgatggcgac
    11041 gacgctgtca aatccgagtt ccgctgcctc gaccgccatc cgcgcaagcg tcgagtcgcc
    11101 ggcgggatag ggacgtacgc tcgcatctgt gatcttcatc gtcgagaggc cgtatgtaaa
    11161 aagagggtta tttatttacc gcggttcgcg tgggagcgga tgctcgggcg ggtcttctcg
    11221 gtcccgcgcc cgcgcgtccg catgccgcgg cccttcatgc cggcggaggt cttgccgcgc
    11281 tccgcccggc cccggtgagt cgggtcggcc agccacgcaa ggttgcggtc gctccggatc
    11341 gatgggtggt gaccgtcgac gaggatgacc tcgaaccatt tggaccgtcc gtcttccccg
    11401 acccagtagg agttcagggc ctccatgttc gggaacttgc gggatgcgcg ttcctcggcc
    11461 atacgctgca ggctctttgc cggggtcatc cggcgcatgc ccatgcgtgc tgtcctgcgc
    11521 ccgcggatgt accggggttt cctccggccg ccgcggcgga ccttgacccg cacgacgatg
    11581 ataccctgtt tggccttgta tccgagcgat cgggcccggt cgatacgggt tgggcgctcc
    11641 acgcgcacga tgctcccctc gcggcgccac tgctggaggc gctcccagag gagactcttt
    11701 acttccgacc tgtccggccg tttccatgcc tcgcgaacgt aggcatacat cgattttgcc
    11761 attctcagat cacctcaggt tcagcgtttc cgccacattc ctttccggga cgtatcccgt
    11821 ggttcctaac aggttgacgg gagcgctaat aaagaattct cttctgccgg ctcccgcgcc
    11881 tacaacccct tgcgcttctc cacgacgcgg atggcatcga tccgggtgac ggggtactgc
    11941 ccgttctcgt ctttttccgc cgatttgacc atatcccaga cggtgaggag agcgaccgag
    12001 acaccggtga gggcctccat ctcgacgccc gttcttccgt aggacttcac gcgggcggtc
    12061 gcctcgatgt agccgtcgcc ctctgcaaag tcgatcgtga tgcccccgag aggtatgggg
    12121 tggcacatgg ggatgatgcg cggcgtatcc ttcaccgcga gggtggccgc cacccgtgcg
    12181 gtcgcgagca cgttgccttt gatcgttgtt ccttcgcgga tggcccggag cgtctcttcc
    12241 cggaggtaga tcctgccgct tgcaaccgct tcccggggga cctcggcctt cgccgtgatg
    12301 tccaccatct gcgcgcggtc gtcctcgata tgggtgaaga ccggggtcct gccgtgtctg
    12361 ccggactcgt tcatgggtat cgcaggagat cgtctgtctc ttgagggata aaagagggcg
    12421 tctccaggaa attataccgg gaagagctcc tccggtatga agtcgagcat atcggtggcg
    12481 agcataccgc atccccgccc ctctgcggca cgcatgccgg ctcttccgtt cacgtaggcg
    12541 gcgatgccgg ccgcctcgaa ggccgggagg cggcagaaga gcgctcccgc aatcccggcg
    12601 agcaggtcgc ccgtcccccc gacggtcatc gccggcgtcc cggtccggtt gaacctgacc
    12661 cgcgccccgt cggatatgac atcgaccggg cctttgagga ggacggtccc cgtcgttctc
    12721 gcgcccctca cgcaccgggc gcgtgcggcg aggtccgccg ggggttccct cccggtcatc
    12781 cgggcgaact cgcccgcgtg cggggtgtat atcgtcctct ttgcggccgg cagggggcgg
    12841 gcgagcgcat cggcatcaaa gaccgcccgc tctgccgcct ctgcgatggc gagcacgacg
    12901 tcgtggctct ctttcccgag gcccatgccg cagacgacga cgtcggtgcg gtcgacgagg
    12961 gagaggatcg tcgcgaggtg ctcttcggtg attaccttcc cttccaggcg ctcgtagata
    13021 aggtcgggca tggggatata ggccggggag gcgatgcgca cgatgtcggc cccggcacgg
    13081 agcgccccca tcgccgcgat gtagggcgct ccctggtagg gtccgccccc gatgacgaga
    13141 acctcgccgc cggcgccctt gtgcgctccg ggcgagcgcg ccgggacgag caggaggtcg
    13201 ccggggccgg tgtaaatctc tgcatcaagc gggatgccga tatccgcgac gtccgcgccc
    13261 tcgacttttg gccggtgaaa cgagaggatc cggttcgccc ggatgcccgg ggtggggata
    13321 tcgacggcga cgatcggtgc cgggctcgcg tttgccccgg caacgagggt cgcgagcggc
    13381 tcgcggaccg cgcccgatgc cccggtcccg agcatggcgt cgacgatgac atccgcttcc
    13441 tcaaagaggc ggccgagggg tttgacgtcg gtcgcgcacc tgaccgggtg gagggcggcc
    13501 gtgcagtgcc ggaggaggga tgcctgggcc gcggccgaag gggtcatcga accgcattcc
    13561 gggtagacga cgtcgaccgc accgatatgc tggaggtacc gggccgcaac catcccgtcg
    13621 ccgccgttgt ttcccctccc gcagaggatg aggatacgat cggggtcgct cgcgaggacg
    13681 gcatccgcga ccgcccggcc ggcgctctcc atcatgagga gcgacgggta gccgagcgca
    13741 acggcgttct cctcgatcgc ccgcatccgg cccgcatcga tcacgccggt ctcccgaaac
    13801 tcccgcaatt cgcctgttgc catgtatcgt tcctccggag cgttgcctga tgggtgctat
    13861 gatagttctc tcttagatac cttttgcgtg ggattctccg gggaaccggt tcgagacacc
    13921 atggtttatt gatactcgcg gcgcactact atggagatgg gcctcgaacg tgacgtgcaa
    13981 agggcagtag atcggatctc tgaagccgac tcggtgacga tcatctccca catcgacgcc
    14041 gacggcatca gcacggagtc gatacttgcg caggcgctcg cccgtgaagg tatacccgcc
    14101 acttccgtct tcgtccgcca gctcgagccg atggcgatgc gccacatccc ggatgacgat
    14161 tccctgaaga tcttcaccga cctcggggcc ggccagcagg cccttcttga ggagcacggc
    14221 ctctctgcgg atggcgtcct gatcatcgac caccacgtca gccagccctg cggcacggcc
    14281 tatccccagg tgaactgcct tgacttcggc atcacccgga tgagcgccgc gggcatcgcc
    14341 tacatggtgg cgagggcgat cgattcggca aacaccgacc ttgcgaagct tgccgtcgtc
    14401 gggaacgtcg gcgacatgat ggcccgcgag aactgtggac tggtcggtcc ggcgcgcgag
    14461 atcgttcagg acggcgtgga gtacggcaac gtaatcgtgc agagagcgga cctgaactgc
    14521 tatgggatat ccacccgccc cgtccacgtc tgcctcggcc gctgcgacga cccttacatc
    14581 gacggtatat caaacagcgt gacccgggcg ctccggttcc ttgagaggct gggtgttacg
    14641 ctgaagacgc cgcggggcgg ctggctcgtc tgggaggagg ttccgctcga tgaccggcgc
    14701 acgatcatca gcgcccttac ccagcagctc gtcgcgcacg gaagagaggc cgaccgcctc
    14761 ctcgccgaga cctacatctt ccccgacgaa ccggaacgga caccgttccg gaacgcctcc
    14821 gagtacgcca ccctcctgaa cgcctgcggc cggtgggcaa aaccgaaggt cggggggcgt
    14881 atctgccgcg gagaccgcgg ggatgcctac cgtgaggcag agtacatgct cgcccaccac
    14941 cgctcggtca ttcgcggcct gctccagtac atactcgaga ccggggtgac ggagctctcc
    15001 cacctccagt acatccacac cggcgaccgg ttccccgaca ccatcgtcgg tatcggggcg
    15061 ggcatggcgc tctccaaact gaactggaag aaaccgatca tggtgctcgc cgcgatggtg
    15121 gatgagccgg atgtcaccaa ggtctcgatg cggacgaacg aatgggcgct cgcccgggga
    15181 gtcgacctgc aggcggcgct cgtcgaggca tcggcaacgg tgggcggcgc gggcgggggg
    15241 caccggatcg cggccggggc gttcatcccc cacgacacag aagaggagtt tatcgatggt
    15301 atcaaccgaa tactcacgcg acagttcgct tcggcggata cggacaatag ctgacttcca
    15361 gttcggcgcc gggtcgggag aggccctctt tcccgaaggg tgcacgtttg agttctcgac
    15421 gaccggacgc atccgccagg tgcgcctcgg gaacgagaga ctcgtgaccg tccgggcaca
    15481 ggacggccgc ctgaccctcg ggatcgccgg agcggcgcgc cttgccgctg ttctcgcccc
    15541 cccgacctac cgggtgacgg tgcgggagga tgtggcggcg ttcatcgcag acgggaagaa
    15601 tgcgatggca aagcacgtcg tcgctgccga tgagggtatc cgtgcagggg acgaggtgct
    15661 ggtggtgacc ggggacgaca tgctccttgc caccggtgca gccctgctct ccggccggga
    15721 gatactggca tttaattacg gtgtagcggt aaaagtacga cagggaagag gatccgaatg
    15781 tttccaggca agataaaccc gagaaaaatg aagcagatga tgaagcagat gggcatggag
    15841 atggaggaac ttgagggcgt cgagaaggtg gtgatctaca cgcccgccgg gaactacgtc
    15901 tttgacgatg cccaggtcgt cgcgacaacg atgcaggggg tcaccaccta ccagctcacc
    15961 ggggaggccc ggtttgaaga ggcggtcccg gagattcccg acgaggacgt cgacctcgtc
    16021 gcatcccagg ccggtgttgc cggagaggtc gcgaagaagg cgctcatcga gacccgcggc
    16081 gacatcgccg aagctatcct gaagctcacg cagcaatgat cgagaccggc gaacagatcc
    16141 tgctcgtcgg cgaggaccgc gagtacttcg tgacggcggg cgaggggcag ttctccaccg
    16201 accgggggat gatcgaccgc ggggcgctcg tcggcatgag cccgggcgat gagatcagga
    16261 cccacctcgg cgctccgttc acggtcctct gcccccggtc gaccgacttc tttgtccacg
    16321 cgaaaaggag cggcgctcct atgctcccaa aggacatcgg caccgtgatc gcttacacgg
    16381 gtatgaaccg cgacgacacg gtcctcgatg ccggcaccgg gagtggtgtt gccgcgatct
    16441 atttcggcaa gatcgtccgc gcggtgaaga cctacgaggt gcgcccggag ttcgcccgcc
    16501 tcgcggagaa gaacatcagg gccgcccgcc tcgataacgt cgatgtgatc gcggccgata
    16561 tgctcacggc aaccggcgcg ttcgacgtcg tccacctgga cctcacgata acgccggcgc
    16621 acgtggagca cgccttctcg ctcctctcgc ccggcggcta cctcgcgacc tacacgccgt
    16681 tccttgagca cacgttcgtc gcgctcgatg ccgcaacgcc gctcttccgc gacgtccact
    16741 gctacgagtg catggagcgc gaactgacgc gctcggcccg ggggacgcgg ccgtcgacca
    16801 gggtcgccca cagcggctac atcacgatcg cacgaaaata ggggttagta ctctttcagc
    16861 gggcgttccc acccggtgta gccgcagtat atgcagcaat caccgccgca gtgcgtgcac
    16921 ttctgtggcg gcttatgcac catcacggtg ccctttttgt tacagtacag gcatccaagt
    16981 ccgccgcagt gacagcagac ctcggagaca tactcttctt ctggttcagg cactgatctc
    17041 cctcccggaa tagtatgagg gggcagaggt gacaaaggtt gtcatgaggg atcacgatcc
    17101 tcgaaaaaaa gtattagtgc gtttgggatg aacctgtatg caggagagaa ggggggcttc
    17161 atccgcacca cagcaaggga aggacggatc ttctgcccgc acgccggccg gacgaaagac
    17221 ccccggaagg taacccacga tgtaccacat tatctctatc cacgattttg agaggagcga
    17281 tcttgattac ctgctcgacc gggcgaaaga gttcgacacc gggggatacc ggtcccgggc
    17341 gcttgaggat aaacttgccg ccctcctctt ttttgaaccg agcacccgga cgcggatgtc
    17401 gtttgcgacg gccatggcgc gccttggcgg acggtggatc agcgtggact cggtcgaggc
    17461 gagttccatc gtcaagggag agacgctggc cgacaccatc agggtgatga gcaactacgc
    17521 ggacgccatc gtgctccgcc atccaaagga aggagcggca aggcttgcaa gcgagtttgc
    17581 cacggtgccg gtcatcaacg ccggggacgg cgcggggcag cacccgagcc agacgctgct
    17641 cgacctctac accatccggc aatcgatgcc gatcgacggt atcgacgtcg ggctcctcgg
    17701 ggacctgcgc tacgggagga cggcacactc gctcgctctc gcgctcgccc attacggtgt
    17761 cacgctccat acgatagcgc cgggagggct tgagatgccg gcgagcataa ccctcgaact
    17821 tcgcgagcac ggcatggagg tggtcgagca cgcggatgtc ggggaggtga tccgggatct
    17881 cgacgtcctc tacgtcacgc ggatccagcg cgagcggttc ccggattcgg cgtcgtacta
    17941 caacgtcgcg tccagttacc ggatcacgct cgatcttctt aacggtgtca gggatgacct
    18001 gatgatcctc caccccctcc cgcgcgccgg ggagatcgat tcggcggttg accgcacacc
    18061 gtatgcgcgt tactttgagc aggcaaagaa cggtgtacct gttcggatgg cgctcctcca
    18121 tgaggtgatg aaatgagcag aaaagatccg tcgggtgggc tgcttatcag ccctatcagg
    18181 aacggcaccg tcatcgacca tatcaccgcc ggcgaggcgc tcaacgtcct ccggatcctc
    18241 gggatcaccg ggtcgacgcg ggagtgcctg agcatcgcga cgaacgtcga gagtaaacgg
    18301 ctcgggagaa aggatatcgt caagatagag aaccgggagc tccgcacgga ggaggtggac
    18361 cggatcgccc tgatcgcgcc gcatgcaaag atcaacatca tccgcgatct ccaggtggtg
    18421 gagaagaagg gcgtggagat accgagggtc ctcaggggcg tggtcaggtg cccgaacccc
    18481 gggtgcatca cgaacacgaa cgagccggtg gagagcacgt ttgaggtgct cgacaagggg
    18541 ctgcactgcc tctactgcga ctggctgatc aagaacgata tagcaaacca catcatctga
    18601 tcaggcgtcc gtgccggtcg atctcccccc ggcagatccg ggtgctcgat atccgccggc
    18661 cgtcttcggc aaggacgcat gatatctcgt ggagatccac cctctttttc ccccggttcc
    18721 gccggaggtc gttgatctcc acggcgaccg ggaaggtctc ctcggagacg accagtatgt
    18781 cgaagtcggc attgagggcg ctgccgtagc ggtcggagag cggttcgacc ctccacgctg
    18841 ccgtgtagcc gtgctccctg atgaaggacg cgacgcttgc caggcgttcc cggtaggagc
    18901 ggacgggatg caccttcgcc ccggcgaact cgtcggtcgt cagtccgatg accacctcgc
    18961 cgtcaggccc ggcgagctca aaagaacggg agaggagttt cctgtgcccg gcatgcaggg
    19021 gatcgaacgt ccccccgacc atgactctca tcagtgaggg tttgcccgga gagggtttat
    19081 gggtagtgta tcggttctcc ctcgctaccc ccctgcccgg agaggtgccg gagaagtaag
    19141 atgtttgaag cgcgagtggc aggagggggg taaacccctc ctcgcgagcc ctctcccccc
    19201 gtggcgatag ccaccccgga tacggttgtt ccccggctcc accccggata cggcttccca
    19261 ctgactccat cccgggtacg gctgctcccc ggttccaccc cgggtacggc tgttccccgg
    19321 ttccaccccg ggtacggctg ttccccggtt ccaccccggg tacggctgtt ccccggttcc
    19381 accccgggta cggctgttcc ccggttccac cccgggtacg gctgttcccc ggttccaccc
    19441 cgggtacggc tgttccccgg ttccaccccg ggtacggctg
//
LOCUS       DAOSII010000022        19356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_3084_length_19356_cov_47.233511, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000022 DAOSII010000000
VERSION     DAOSII010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 19356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19356
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3084_length_19356_cov_47.233511"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..986
                     /locus_tag="PLV90_03055"
     CDS             165..986
                     /locus_tag="PLV90_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOF96124.1"
                     /translation="MHAWNPDDYHKHSAAQRAWATELIGKLELAGNERVLDLGCGEGK
                     VTAEIAARLPSGSVLGLDISRDMIAFARMHLPPERYPNLRFAEGDMLDLPFDTEFDIV
                     FSNAALHWVSDHGRVFSGIARALRPGGRVLLQMGGRGNAAPVLAVADDMLAGKPWTTL
                     ASGPPSRYAFYGPEDEREPLRAAGLIPVRVELIGKDMAFDSPDGLAGWVRTTWHLYLE
                     RLPEEVRPAFVAELVARYVEGRPSQDGRIHVPMVRLEVEAVKAAKRWSTGNSSLR"
     gene            1093..2487
                     /locus_tag="PLV90_03060"
     CDS             1093..2487
                     /locus_tag="PLV90_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOF96125.1"
                     /translation="MNDILTAQCSNNLLTEGSVGKGLWIVALPIIISNFLASILEVVD
                     MYFIGKLGDVSIAGGAMSISIIIVLTTVIFGTVTATAAFVSRAYGSERYERIPVILSH
                     SLYLALGFSAVLAVIGMFWSQDLLLLLGADPEVAAVGARFLSPMLMGMFVFVTLMILT
                     TVFQSTGDSRTPMFVMIGVNIANIVLNPTLIMGLGGLPAFGIAGSAYASIASRATGVL
                     LLVGVMYLLPSKKNGPVRLPKQWTFEPRLLKDIVRVMVPSAVQSGVRSFAFLGMTAIV
                     ALYGTSAVAAYGICQRLDMLGLIFVMGLCTGVAVMVGQNLGAGKVERAEKTVRIAMIV
                     NALFMALVGILYLRSADHLLAFFGATEASLANGIQFMHIVPPSYFVIAMAMTMGFAMN
                     GAGMTRPGMYAAIAGQLIVQVGLAAALVAMEMPLQFIWFAVVCGTVVVFLCDLFFYRQ
                     GAWKTKKLDLGGEN"
     gene            2779..4188
                     /locus_tag="PLV90_03065"
     CDS             2779..4188
                     /locus_tag="PLV90_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF96126.1"
                     /translation="MDRQQKIVLSVLVLGTLMGALDSTIVILAFPVIADGLHADLLST
                     MWIILVYLLIMASLTVPLGRLGDLFGRPRMFNLGFGLFTVSSFFCGISSSITMLILFR
                     GVQALGGALLESNSGAIIADIFPPNARGAAYGYNSMGWTVGATLGIVLGGIITTFAGW
                     EYIFFINVPIGIVALYLGLRYVKGAARTQASLDLPGMALLTIALSLISYAAIDFAGNG
                     LTTWSTIVAPLGVFFLLVFAWHEVRTRTPMIDFESLNRPILKYSLLAAFFQSLGYLAV
                     IFLITMYLQGIRGLSPLDAALLLTPGYVVGSVLGPFMGRLSDRLGARIIATTGTLVIA
                     AAIFIFMMLRVDSPLSIVLLASFVSGVGTSMFYPANNSAIMANARAGDYGGISGALRM
                     VQNIGILGSFVLAITVASASIPRALAFEIYVGTSHLVGNVAGAFIQGIDAALMVSLLL
                     LLLAGAMSWSRGSEHREAA"
     gene            4373..7768
                     /locus_tag="PLV90_03070"
     CDS             4373..7768
                     /locus_tag="PLV90_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOF96127.1"
                     /translation="MTHRPTSGERRALCGICSAGCGVIVAYDDHGRIVSVRPDEDAEI
                     GIICRLGEASPEIVYSSDRVLYPLRRAGPKGTHAFERITWDEAYETIVANLTRIREES
                     GPEAVAIYTGSGSFELSFCDIFQPKGVAVSSASSVLFPFGSPNTMGVGALCYVSFAMI
                     APHVTMGEMYFNMFSDYEQSDLILVWGTNPATDCPPLALRALAGARRRGAEVVVIDPR
                     RTRTVDLVDAEWVPIRPGTDGALALGLANVIVAEELYDADFVANWCHGFEEFATYVQH
                     YRPEVVESITGIPAETVASLARRIARARGASFAMYTGLEYSDSGVQAIRAVFVLWGIS
                     GNLDVPGGRCFAMRGNAFPINRSGYIQNPDLKRAIGTDRFPVYTHYRQEGHAIALPDA
                     VLRGRPYRIRALILEAAHMLISWPQTPVWRETLANLDFLVSVDRHLTADAAYADIVLP
                     ATTLYERESYMTYGPVFRLRERVIEPLGEARGDVQIMAELARRLGYGHLYPQSEEEAL
                     RHVLKGSGFTLEGVRAAGGTARSPAAMMEYRKWEKGLLRPDGRPGFDTPTGKFEIYST
                     ILEEYGYDPLPVYTEPGESPLSQPEQADRFPLVFNSGARVTTDFHAQHHSIPSLVRER
                     PEPTVTLNSHDARERGIRDGDRVIVRTARGEIVLRALVTDGIVRGAVEANMGGGCHQA
                     PEAWREGNVNELTDLARYDPISGFPVYKALLCDVARAEGDGGRVAIGTGEVDSVAGAA
                     PPPEAPRIYLDHNATTPLDPAVHQAMAAYLASNPGNPSSIYREGRDAKVAVESARRSL
                     ARLLNCTARRITFTGSCTEANNTVIKGLAFAGRDGIRREIITSPTEHSAVIEPCRWLE
                     QFGFRVTFLPVDRSGRVDPADLEGLIGPETLLVSVMVANNETGTIQPVRELVRIAHDH
                     GALFHTDATQAIGKMPVDAVDLDVDFLTLSGHKIYGPKGVGALYMKKGVRIAPLVHGG
                     EQEGRFRAGTENVAGIVGLGRAAEIAEQHIGRMDAVRHLRDRLEAGIRETVLGARLNG
                     HPTERLANTLNMVLPGYRGESIVLALDRRGISLSSGSACHSGSPAPSHVLLAMGLSAE
                     DAHCSVRMTLGAHTTDEDIDRALEAFRDLLHGPGEIIRFVACR"
     gene            7835..8905
                     /gene="egtD"
                     /locus_tag="PLV90_03075"
     CDS             7835..8905
                     /gene="egtD"
                     /locus_tag="PLV90_03075"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR03438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HOF96128.1"
                     /translation="MTGRLSTPSVRSELRNYLISAYRDDLASDILSGMTAEQKYIPSR
                     YFYDARGSQLFEEICRLPEYYPTRLELSILRGACGALMESFGEGDLVELGSGANKKIS
                     LLIDAAPGPEGEGLRYIPVDVSESALAAATEGLLWGYPHLHVLGIVGDFTRDIERIPR
                     GRKRLITFFGGTVGNFTGPEAHRVLANVAAMMEEDDRFLVGLDMVKPVPVLEAAYNDT
                     AGVTAAFNRNILAVVNRELGADFDPGLFEHRAFYNRDLAQIEMHLMAREAMTVRIPAL
                     GLRVAFERGETILTEISRKFTRESAGRLFGAAGLAVERWFSDPRGWFALASLSRAAGR
                     PVEPAGMAQDPPARGGPAGIHR"
     gene            8980..9813
                     /locus_tag="PLV90_03080"
     CDS             8980..9813
                     /locus_tag="PLV90_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S16"
                     /protein_id="HOF96129.1"
                     /translation="MRVREKTLTILFVLSLFANVFLLAVALVPGDDIPALFPPDAGPS
                     PAATMASLPGIGRTASMQAPVTLQKVEITGIGPFSTNRMIEEGAMVNISVEIVPGRGR
                     VLVQTTPLMGIVFQDAANRAVDLAAGRSHADLAGSDIIFSIQSPDGVSEIDGPSAGAL
                     MTALLLSVMEDFALNESVTVTGTIDENGNIGPVGGILEKAGAAAASGKTLLLLPAENN
                     NIVESREETGIFCGLRIARQRPVAVDTGMYIEERYGIRVKYVHTLDDFLADVCTPAAT
                     P"
     gene            9879..10472
                     /locus_tag="PLV90_03085"
     CDS             9879..10472
                     /locus_tag="PLV90_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96130.1"
                     /translation="MKPQSLLIIGVLAVALVAVLAILPPGPAAPQPPGPGEGEPAPAP
                     TPAPLPENGTYINTTYGYAVTCPAGWFFTESGESVWFSSPVKHEEVRINTIPLPDGNS
                     SREAILETLNATYAEDLGTDIGAVWVSTEKTALDGIPAYRSVYSVPIVESDRYTFIIR
                     YAVRDDIVYSVMHTVFPEGYDSYNADAAAVAESFEFI"
     gene            complement(10587..10751)
                     /locus_tag="PLV90_03090"
     CDS             complement(10587..10751)
                     /locus_tag="PLV90_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96131.1"
                     /translation="MTLLQQIRLWMQTRGEGKQLHDCENDPIIEPYFDLSPEATLPPE
                     TRENLLEEDR"
     gene            complement(10923..12668)
                     /gene="mutL"
                     /locus_tag="PLV90_03095"
     CDS             complement(10923..12668)
                     /gene="mutL"
                     /locus_tag="PLV90_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOF96132.1"
                     /translation="MTKIRILDPDTVNQIAAGEVVERPASVAKELLENAIDAGATSIL
                     LDVGSDMAGVTKIRVTDDGAGMTAKEALLAFHPHATSKIQDIADLSTVRTLGFRGEAL
                     ASIAAVAEVTLVTRPRGAGALAGTRVVVRGGEVVETGDVGAPEGTTIAVEHLFYNTPA
                     RRKFLKSRNTELAHLYGVVENLSLAHRGIAFRVVHNGKERMAVQRSGELIATIAGLYG
                     ADLARALVPVRGRLPFLSIGGYVSRPSESRGNPSQVVISVNGRSISSRPIAAAVREGY
                     GTLLPKDRYPVAFIDLAIDTDLVDVNVHPTKREVRLSREREILAAVAAAVEEALLGHD
                     LAGATPAEPVQQVLTGERPEAAPAAREAEAAYAAGHRDIARSDRQLRRTEGEGEENLL
                     PAMEPVGQVAATYIVAEGVDGTLYLIDQHAAHERILYDQVAERRDAAPGSQELIMPVV
                     LSLPPKESAALRDAMPFLAEEGFEVEEFGRDTFAVRAVPAALGAMEDPESIRETIADI
                     LAGESRTTPGRREAVTCIVACRGAVKAGTLLTHDQQRRLLSQLARTKTPWTCPHGRPT
                     VVAFDRRRLDGMFGR"
     gene            complement(12665..15283)
                     /gene="mutS"
                     /locus_tag="PLV90_03100"
     CDS             complement(12665..15283)
                     /gene="mutS"
                     /locus_tag="PLV90_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOF96133.1"
                     /translation="MTDGPTPAMRQYYSVKNRYPDAIIFFRMGDFYETFGEDAGVVAR
                     ELDITLTARGKDRKGDRMPLAGVPHHAADGYIARLVGRGYKVVICDQVEDPKTAKGVV
                     KREVTRVITPGTLIDSSMLGSAGARYLMAVAPDRKDTFGLAFLDVSTGEFFVSAGSGG
                     REYADVVSEAVRYRPSEAILPEALDEGLAGRLESIGVTVSRYRDDAFDPDAACRLLCE
                     QFGTATLDGYGCAQMTGAVAAAGAALHYARETQQSPLPHITGLSTRVPSGTMVLDAIT
                     LRNLEITTGIRGEGDRSTLLAALDVTETSMGSRTMRSFLVAPLVRKAAIEERLDAVEW
                     LIGHTVERQALRAALGDFADIERIAGRIAYGNAGPRDLAMLRESLSAVPGVKTLLPGD
                     LPTLVREALDAMSDHASASDLIGRAIVDDPPALAKSGGMIREGFNAKLDELRGLATTG
                     KDWIVEFQQQERERTGIRSLKVAYNRVFGYYIEVTKPNLPLVPPEYERRQTTANGERY
                     TIPDLREKEALIATAEERLSALEAEIYAELVRTLAAEVPGLQATARAVGLLDVYSALA
                     EVAARYGYSRPAIEESGRIVIRDGRHPVVERHLPVPFVPNDTELDSAGDQIMIITGAN
                     MAGKSTYMRAVALCCIMAQMGCFVPARHATIGIVDRVFTRVGAFDDLASGQSTFMVEM
                     LELANILNNATPQSLVVLDEIGRGTSTLDGCAIARSVVEFLHGRAIAGPRTLFATHFH
                     DLIDLEGSQKRVKNFHFAVKDTGQDVVFLRKIIPGATDRSYGVHVARLAGLPKKVTDR
                     AMDLLKEASVRELPTGVRAPRYTQMLIVDPGEGAVAENPAVGELKRLNPDEMTPIEAL
                     NALCRLRRMANGEGER"
     gene            15430..16683
                     /locus_tag="PLV90_03105"
     CDS             15430..16683
                     /locus_tag="PLV90_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOF96134.1"
                     /translation="MAATIAEKIFSMKCGRSVRAGDVVMAPLDAAMIHDITGPLAVRV
                     FGEMGGERVFDPERIIMLFDHQVPADSIPAAENHVFMRRFAEEQGIHNYDLLEGVCHQ
                     VVMEKGRAAPGEIIVGTDSHTCTYGAAGAFATGIGSTDMGFVLKFGALYFRVPESILL
                     EVDGVFDRRVGAKDLILSLAGDIGADGATYMTLEFTGKAMERMNMAGRMTCANMAIEM
                     GAKAGIVRPDAATWEYVAARREIEPFDLAGDDDAVYRERRHYDVTDLAPQVAVPHNVD
                     NVVDVADVAGTKVDQVFIGSCTNGRYEDLAEAGEVMGNADRFAKGVRVIVIPASRTEY
                     LKALRAGLIERFVEAGALVEAPCCGPCMGGAFGLLAPGEVSLSTSNRNFRGRQGSAQA
                     SVYLSSPATAMASALYGEITDPREV"
     gene            16688..17179
                     /locus_tag="PLV90_03110"
     CDS             16688..17179
                     /locus_tag="PLV90_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HOF96135.1"
                     /translation="MRVWKFGDDIDTDAIIPGRFLTIYDPAELAKHAFEGTRDEFARD
                     VREGDVVVGGTNFGCGSSREHAPLALLGAGVRVVVARSFARIFYRNAVNTGLLPLVAA
                     GADSIPDGAAVSVDIAGGFVEVDGRRLVVETVPGFLKEIVDAGGLVAYAKNLDEVKTC
                     STE"
     gene            17164..18285
                     /locus_tag="PLV90_03115"
     CDS             17164..18285
                     /locus_tag="PLV90_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HOF96136.1"
                     /translation="MQHRVAAIGGDGIGPEIVAAGREVIDAAGERYGFDIAWTDFDVG
                     AERYLATGELLTEEDIRELSKFKAIYFGAIGDERVKPGILEKGILLAIRFHFDQFVNL
                     RPIRLLKGVATPLAHKRPEDIDFVVVRENTEDFYVGIGSRFTGKRESKTLEVVRDLYN
                     VKFGLDVETDADELAYQIGVVTRPGAERVIRYAFDLAGRRRGKLTSVDKANVLSDVYG
                     LWRDVFTGVAAGYPDVQTEFNFVDAVTMWFVKNPEWFDVVVTPNMFGDIITDLGAMIQ
                     GGLGLAPGGNINPAGTSMFEPIHGSAPKYRGQDVANPLATIWAGSMLLDHIGEHEAAK
                     AVLSAVEQSILDGFVTRDMGGAKKTSEVGRYVASLVRGV"
     gene            18326..>19356
                     /locus_tag="PLV90_03120"
     CDS             18326..>19356
                     /locus_tag="PLV90_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOF96137.1"
                     /translation="MPENQHTPCENPLWPCAMTGAVACLAGFEDLAVVVHGSSGCYFY
                     PASLVGVPVHGTFLIENEIIFGTESRLAEVIHDLEGRYSRIAVVNTCVPAIMGEDIGA
                     LARDGRVLIVDSPGFIGDLEAGYRRALDALAPEVDEGIAGVNIDGICRTDPFCRGNAI
                     EARRLLDLAGASVGTTFCLDRFDAAHRAAPFTVGTNPDLASGVGTACGSLLGLDAVRE
                     TFERLGAVIGGIDTRPVAEEVAWADERIRKACEKYLRRFDPPRAAIAAQSAYAAFAAD
                     LLDRYLGADIACVAARNDPGEGGAAAVRTTDFAVLQERMTGAEPDLVIGSSYERAMAP
                     GAAFVGLTPP"
BASE COUNT         3157 a         6355 c         6341 g         3503 t
ORIGIN      
        1 gagaccctct tcgtgatccg ggcgatgcgg gacctctgcc ggatgggggc gtgagtggtc
       61 cccggggcaa aacccgctga tgcacttttc cggcgcgctc cctcaccgat ggcttgatgc
      121 cctgcggcag cggtacccgt accagcggag agggtattcc tgtgatgcac gcgtggaacc
      181 cggacgacta ccacaagcac tccgccgccc agcgggcttg ggcgacggag ttgatcggaa
      241 agctcgaact tgccggcaac gagcgggtgc tcgacctcgg ctgcggcgag gggaaggtga
      301 ccgcggagat cgccgcccgc ctcccctcgg gctcggtgct cggccttgac atatcccgcg
      361 atatgatcgc cttcgcgagg atgcacctgc ccccggagcg gtatccgaat ctccggtttg
      421 ccgagggcga tatgctcgat ctgccgttcg atacggagtt cgacatcgtc ttctcgaacg
      481 ccgccctgca ctgggtctcc gaccacggca gggtcttctc ggggatcgca cgcgccctcc
      541 ggccgggagg acgggttctc ctccagatgg gcgggagagg gaacgccgcg ccggtcctcg
      601 cggttgcaga cgatatgctg gccgggaaac cctggaccac gcttgcaagc ggaccgccgt
      661 cccgttatgc cttttatggg ccggaagacg aacgtgaacc gctccgggcg gcagggctca
      721 tccccgtgcg ggtcgagctg atcgggaagg atatggcctt cgatagccct gacggcctcg
      781 cgggatgggt ccggacaacc tggcacctgt acctggagcg gctccccgaa gaggtccggc
      841 cggcgttcgt cgccgaactg gtggcccggt acgtggaggg gcgcccctcg caggacggcc
      901 gcatccacgt tcccatggtg cgcctcgagg tagaagccgt caaagcggcg aagcgatggt
      961 ccaccggcaa cagctctctt cgataacata tcctccacag cccgatcaaa agccataacg
     1021 cttcttttca catttacgga aaaatgcagg ttgaggcggt cggtcaaact ttatcgtctt
     1081 ccgccccgta taatgaatga tatcttgacg gcacaatgtt ccaataatct tctcaccgag
     1141 ggatcggtcg gaaaaggcct ctggattgtg gccctcccga tcatcatcag caatttcctt
     1201 gcgagcatcc tggaggtggt ggatatgtac ttcatcggca aactcggcga tgtctcgatt
     1261 gccggcggag cgatgagcat atccatcata atagtgctta ctaccgtgat cttcgggacc
     1321 gtgacggcaa cggctgcatt cgtgtcgcgg gcgtacgggt cggagcggta tgagcgcatc
     1381 ccggtgatcc tctcgcattc gctgtacctg gcacttggct tctccgcggt ccttgcggtc
     1441 atcggtatgt tctggtcgca ggaccttctc ctgctcctcg gagccgatcc cgaggttgca
     1501 gcggtggggg cacgcttcct ctccccgatg ctgatgggga tgttcgtctt cgtcaccctg
     1561 atgatcctga cgaccgtgtt ccagagcacg ggggactcga ggacgccgat gttcgtcatg
     1621 atcggggtga acatcgcgaa tatcgtcctg aacccgacgc tgatcatggg ccttggcggt
     1681 ctcccggcgt tcggcatagc cggatccgcg tatgcgtcca ttgcgtcgcg tgcaaccggc
     1741 gtgctgcttc tcgtcggggt gatgtatctc ctgccgtcga agaagaacgg tccggtcagg
     1801 ctcccgaagc agtggacgtt tgagccgcgg ctgctcaagg atatcgtgag agtcatggtg
     1861 ccgtcggccg tgcagagcgg ggtcaggagt tttgcgttcc tcggtatgac ggcgatcgtg
     1921 gcgctgtacg gcacgtcggc ggtggcggcg tacggtatct gccagcgcct ggatatgctg
     1981 ggcctgatct tcgtgatggg gctctgcacg ggagtcgcgg tgatggtcgg gcagaacctg
     2041 ggagcaggga aagtggagag ggcggagaaa acggttcgga ttgcgatgat cgtgaacgca
     2101 ttgttcatgg cattggtggg catcctctac ctgcggtctg cggatcatct cctggcgttc
     2161 ttcggcgcga ccgaggcgtc gcttgcaaac ggcatccagt tcatgcatat cgttcccccg
     2221 tcgtacttcg tgatagcgat ggcgatgacg atggggtttg cgatgaacgg tgcggggatg
     2281 acgagaccgg gcatgtacgc ggcgatcgcg gggcagttga tcgtgcaggt gggccttgcc
     2341 gcggccctgg tggcgatgga gatgccgctg cagttcatct ggttcgcggt ggtctgcggc
     2401 accgtggtgg tcttcctgtg cgatctcttc ttctaccggc agggggcctg gaagacgaag
     2461 aaactggatc tcggcgggga gaactgaccc ggaaaacccc cgacaacctt tttttcgact
     2521 gggccggcac gccgctgagg tcttgaagaa ccctcgcagg gggtcaggct gccgatctcc
     2581 ctgcgcggtt ttgcgctcga cacgaagggt gccggtcgtc aagatgagca agatccggtg
     2641 cctgcgctct cgctgcatcg ggcggttgca acccgttctc acctccgggc tctccttctt
     2701 cacgatgggc agaaagaccg atatgcacgg acgccaattt gggaacccct atatggatac
     2761 acaatctaac ctctcgatat ggatcggcag cagaagatcg tgctctctgt tctcgtgctc
     2821 ggcaccctga tgggggcact tgacagcacc atcgtcatcc tggcctttcc cgtcatcgcg
     2881 gatgggctcc acgcagatct tctcagcacc atgtggatca tcctcgtcta tctcctgatc
     2941 atggcgtccc tgaccgtccc gctcgggaga ctcggggatc tcttcggccg gccccgcatg
     3001 ttcaacctcg ggttcggcct cttcaccgtc agttcgttct tctgcggtat ctcctcatcc
     3061 atcaccatgc tgatcctctt ccgcggcgtc caggccctgg gaggggcact cctggagtcg
     3121 aactcaggag ccatcatagc cgatatattt cctcccaatg cacgcggggc agcatacggc
     3181 tacaactcca tgggctggac ggtcggtgca acgctcggca tcgtgctcgg gggcatcatc
     3241 accacctttg ccggatggga gtacatcttc ttcatcaacg tccccatcgg catcgtcgcc
     3301 ctctaccttg gtctgcgcta cgtgaagggt gcagccagga cgcaggcatc cctcgacctc
     3361 cccggcatgg ctctccttac catagccctc tcgctcatat cctacgccgc gatcgacttt
     3421 gccggaaacg gcctcacgac gtggtcgacg atcgttgccc ccctgggggt cttcttcctc
     3481 ctggtatttg cctggcacga ggtgcgcacc cggaccccca tgatcgattt cgagagcctg
     3541 aacaggccga tccttaaata cagccttctc gctgcattct tccagagcct tggctacctc
     3601 gcggtcatat ttctcatcac catgtacctc caggggatcc gcgggctctc ccccctggat
     3661 gccgccctcc tcctcacgcc cggttacgtc gtggggagcg ttctcggccc cttcatggga
     3721 aggctctcgg atcgcctcgg ggcacggatc atcgccacca ccggaacgct ggtgatcgcg
     3781 gcggcgatct tcatcttcat gatgctcagg gtagactccc ccctctccat cgtcctcctg
     3841 gcgtcgttcg tctccggtgt cggcacatcg atgttttacc cggcaaacaa cagcgccatc
     3901 atggccaacg cgcgggcggg ggattacggg ggcatatccg gcgcgctccg gatggtgcag
     3961 aacatcggca tcctcggcag tttcgtcctt gccataacgg tggcctccgc ctcgatcccc
     4021 cgcgctcttg cgttcgagat ctacgtgggc acatcccacc tggtagggaa cgtggccggg
     4081 gcattcatcc agggcatcga tgccgcgctc atggtctccc tcctcctcct cctccttgcc
     4141 ggggcgatgt cctggtcccg cgggtcggag caccgcgagg cggcatgacc cgcccccata
     4201 ccccggccgc ccgatccggt ggtcgtgcgg gtagcggcag gataccggat agccacggca
     4261 tccccggaaa gactcctgcc ggcctctcct gatccggcca tggtaaccat tatgccgcac
     4321 cggccccagg gggcggttcg tgcaaccggg gtataccatg cagggatcac acatgactca
     4381 ccgccccaca tcgggggagc gccgggcgct ctgcgggata tgctctgcgg ggtgcggcgt
     4441 aatcgtcgcc tacgatgacc acggaaggat cgtctccgtc cgccccgacg aggatgccga
     4501 gataggcatc atctgcaggc tcggggaagc ctcgcccgag atcgtctact caagcgaccg
     4561 ggtgctctac ccgctccggc gcgcagggcc gaaaggcacc catgcgtttg aacggatcac
     4621 ctgggacgag gcatacgaga cgatcgtcgc gaacctgacc cggatcagag aggagtccgg
     4681 gccggaggcg gtcgccatct acaccgggtc ggggagtttc gagctctcgt tctgcgacat
     4741 attccagccg aaaggtgtcg cggtctcgtc cgcatcgagc gtcctctttc ccttcggctc
     4801 cccgaacacc atgggcgtcg gggcgctctg ctacgtctcc tttgctatga tcgcgccgca
     4861 cgtgaccatg ggggagatgt acttcaacat gttctccgac tacgagcaga gcgacctcat
     4921 actcgtctgg gggacaaacc cggccaccga ttgcccgccc cttgccctcc gggctctcgc
     4981 cggggcgcgc cggcgggggg cggaggtcgt cgtcatcgat ccccgccgca cccggacggt
     5041 ggacctcgtg gacgcggagt gggtgcccat ccggccgggg acggatggag cgctggcgct
     5101 cggtctcgca aacgtcatcg tcgcggagga actctacgat gccgatttcg tcgcgaactg
     5161 gtgccacggt tttgaagagt tcgcaaccta cgtccagcac taccgccccg aggtggtgga
     5221 gagcatcacc gggattccgg cagagaccgt ggcgtcgctt gcccggcgca tcgcccgcgc
     5281 ccggggcgca tcgtttgcca tgtacacggg gcttgagtac agcgactccg gcgtgcaggc
     5341 catccgggcg gtcttcgttc tctgggggat atcggggaac ctggacgtcc ccggcggccg
     5401 gtgttttgcg atgaggggaa acgccttccc catcaaccgc tccggctaca tccagaaccc
     5461 cgacctgaag cgggctatcg ggaccgaccg gttcccggtc tacacccatt accggcagga
     5521 gggccacgcg atcgccctgc cggatgcggt ccttcgcggg aggccctacc ggatacgcgc
     5581 cctgatactg gaggccgccc atatgctgat atcctggccg cagacgccgg tctggcggga
     5641 gaccctcgcg aacctggact tcctggtctc tgtggaccgg cacctgacgg ccgatgccgc
     5701 ctacgccgac atcgtcctcc cggcgacgac cctctatgag cgggaatctt acatgaccta
     5761 cggcccggtc ttccggctca gggaacgggt gatcgagccc ctcggcgaag cccggggcga
     5821 tgtccagatc atggcggaac tcgcccgccg cctcggttac ggccacctct acccgcagtc
     5881 ggaagaagag gctctccggc acgtcctgaa aggctcgggg ttcacgctcg agggcgtcag
     5941 ggcggccggc gggaccgccc ggtcgccggc ggctatgatg gagtaccgga agtgggagaa
     6001 aggcctcctc cggcccgacg gccggcccgg gttcgatacg ccgacgggga agttcgagat
     6061 ctattcgacc atcctcgagg agtacggcta cgatcccctc ccggtctaca ccgagcccgg
     6121 cgagagccct ctctcgcagc cggagcaggc agatcggttc cctctggtct tcaactccgg
     6181 agcgcgggtg acgaccgatt tccacgccca gcaccacagc atcccctcgc tcgtcaggga
     6241 gcggccggag ccgacggtca ccctcaactc ccatgacgcc cgagagcggg ggatccgcga
     6301 cggcgaccgg gtcatcgtcc ggacggcgcg gggcgagatc gtgctccgcg cactcgtcac
     6361 cgacggtatc gtccggggcg ccgtcgaggc gaacatgggc ggcgggtgtc accaggcgcc
     6421 ggaggcctgg cgggaaggaa acgtgaacga gctgaccgat cttgcccgat acgacccgat
     6481 atcggggttc cccgtctaca aagccctcct ctgcgatgtg gcgagggccg aaggcgacgg
     6541 cgggagggtc gccatcggga ccggcgaggt cgactccgtc gccggggcgg cgccgccgcc
     6601 ggaggcaccc cggatatacc tcgaccataa cgccaccacc cccctcgacc ctgcggtcca
     6661 ccaggcgatg gccgcgtacc tcgcgtcgaa cccgggcaac ccctcgagca tataccggga
     6721 ggggagagac gcgaaggtcg ccgtcgagtc cgcccggcgg agccttgccc gcctgctcaa
     6781 ctgcaccgcc cggcggatca ccttcaccgg ctcctgcacc gaggcgaaca acacggtgat
     6841 caagggcctt gcctttgccg gccgggatgg gataagaagg gagatcatca cctccccgac
     6901 cgagcactcc gcggtcatcg agccctgccg gtggcttgag cagttcgggt ttcgcgtcac
     6961 gttcctgccg gtggaccgct ccggccgggt cgacccggcc gatctcgagg gcctgatcgg
     7021 cccggagact ctcctcgtct ccgtcatggt ggcgaacaac gagaccggga cgatccagcc
     7081 cgtccgggaa ctggtgcgga tagcccacga ccacggcgcc ctcttccaca ccgacgcgac
     7141 ccaggcgatc gggaagatgc cggtggatgc ggtcgacctc gacgtggact tcctgaccct
     7201 ctccgggcac aagatatacg gcccgaaggg cgtcggagcg ctctacatga agaagggggt
     7261 ccgcatagcc ccgctcgtcc acggcggcga acaggagggc cggttccggg ccgggacgga
     7321 gaacgttgcc gggatcgtgg ggctcgggag ggccgcggag atcgcggagc agcacatcgg
     7381 tcgtatggat gccgtccggc acctgcggga ccggctggag gccgggatcc gggagacggt
     7441 tctcggggcc cggctgaacg gccatccgac cgaacggctc gcaaatacgc tcaatatggt
     7501 gctccccggc taccgcggcg aatcgatagt cctcgccctc gaccggcggg gtatctccct
     7561 ctcctcgggc tcagcctgcc actcgggctc tccggcgccg tcccacgtcc tcctggcgat
     7621 gggcctttcg gcagaggatg cccactgctc ggtccggatg acgctcggcg ctcacacgac
     7681 ggatgaggat atcgaccggg cactcgaggc cttccgcgat ctcctgcacg gtccggggga
     7741 gatcatccgg ttcgtcgcct gccggtgacc ggagatctcc gcgccatcgg gggagcgggc
     7801 aatatatatg ccataatcga gatcggtgtc gggcatgacg ggtcgcctga gcactccttc
     7861 cgtacggagc gagcttcgga actacctcat ctctgcatac cgtgacgacc tggcctcgga
     7921 tatcctctcc gggatgaccg cggagcagaa gtacataccg agccggtact tctacgacgc
     7981 ccggggatcg cagttgttcg aggagatctg ccgtctccct gagtattatc cgaccaggct
     8041 tgagttatcc atccttcgcg gggcgtgcgg ggcgctcatg gaatcctttg gggagggcga
     8101 cctcgttgaa ctggggtcag gagcgaacaa aaagatctcc ctgctcatcg atgcggcgcc
     8161 ggggccggaa ggcgaaggcc tgcggtatat cccggtggat gtgagcgagt cggccctggc
     8221 cgccgcaaca gaggggctgc tttgggggta cccgcacctg cacgtcctcg ggatcgtggg
     8281 cgatttcacc cgcgatatcg agcgcatccc ccgcgggaga aagaggctca tcacgttttt
     8341 tggaggcacc gtcgggaact ttaccggccc tgaggcgcac cgggtgctcg cgaacgtggc
     8401 tgccatgatg gaggaggacg accggttcct ggtcgggctc gatatggtga aaccggtccc
     8461 ggtgctcgag gccgcgtaca acgacaccgc cggggtgacc gccgcgttca acaggaacat
     8521 cctggccgtg gtcaaccggg aactcggggc ggacttcgat ccgggcctct ttgaacaccg
     8581 tgcgttctac aacagggatc tcgcgcagat cgagatgcac ctcatggcgc gggaagcgat
     8641 gacggtcagg atccctgccc tggggctccg cgtggcgttt gagcgggggg agacgatcct
     8701 caccgagata tcccggaagt tcacccggga gagcgccgga cggctctttg gtgccgccgg
     8761 gcttgccgtg gagcgctggt tctccgaccc ccgcggctgg tttgcgctcg cctctctctc
     8821 ccgggcggca gggcgccccg tagagccggc cggtatggct caggatcccc cggcccgggg
     8881 aggtccggcc gggattcatc gatgaatcgg cgggcaggta cgtgcgcata tccgccaggg
     8941 gaaaagtata tatctggatg aacggtatgc gggcagcata tgcgcgtacg ggagaagacc
     9001 ctgacgatcc tcttcgttct ttcgctcttt gcgaacgtct tcctccttgc cgtcgctctc
     9061 gtgcccgggg acgatatccc cgcgctgttc cctccggatg ccggtccgtc cccggcggcg
     9121 accatggcct ctctccccgg gatcgggaga acggcatcga tgcaggcacc cgtcaccctc
     9181 cagaaggtcg agatcaccgg catcggcccg ttctccacga accggatgat cgaggagggg
     9241 gcgatggtga acatatcggt cgagatcgtg cccggaagag ggcgggtgct cgtccagacg
     9301 acgcccctga tggggatcgt ctttcaggat gccgcaaacc gtgccgtcga cctcgccgcc
     9361 ggccgttccc acgcggacct tgcggggagc gacatcatct tcagcatcca gagccccgac
     9421 ggggtctccg agatcgacgg ccccagtgca ggagcgttga tgaccgcgct cctcctctcg
     9481 gtgatggagg actttgcgct caacgagagc gtgaccgtga ccgggaccat cgatgagaac
     9541 ggcaacatcg gcccggtcgg cggcatcctc gagaaggccg gggccgccgc ggcgagcgga
     9601 aagaccctcc tcctcctccc ggcggagaac aacaacatcg tcgagagccg ggaggagacg
     9661 gggatctttt gcggtctccg gatagcccgg cagaggccgg tcgccgtcga tacggggatg
     9721 tacatcgagg agcgctacgg gatccgggtg aagtacgtgc ataccctcga cgacttcctt
     9781 gccgatgtct gcacccctgc cgcgaccccc tgatcgcccg gggaacggcc actgcaatga
     9841 aggatattta tggtctcccg ggccacgtac cccggagcat gaaaccacag agtctgctga
     9901 tcatcggggt tctcgcggtc gccctggtcg ccgtcctcgc gatcctgccg ccgggccccg
     9961 cggcgccgca acctccgggc ccgggagagg gtgaacccgc tcccgccccg actccggcgc
    10021 ccctgcccga gaacggcacc tacatcaaca cgacctacgg ctacgccgtc acctgcccgg
    10081 cggggtggtt cttcaccgaa tcgggagaga gcgtatggtt ctcttcgccg gtgaagcacg
    10141 aggaggtccg catcaacacg atcccgctcc cggatgggaa tagctctcgc gaggcgatcc
    10201 tcgagacgct gaacgcgacc tacgcagagg acctcgggac tgatatcggg gccgtgtggg
    10261 tctcgacgga gaagaccgcc cttgacggta taccggcgta caggtcggtc tactccgtcc
    10321 ccatcgtcga gagcgaccgc tacaccttca tcatccgtta tgccgtcaga gacgacatcg
    10381 tctactcggt catgcacacg gtcttccctg aggggtacga tagctacaac gccgacgcgg
    10441 ccgccgtggc ggagtcgttt gagttcatct gaccccccgc gatcatcccc gggcaccggg
    10501 cgggttctca aaaagaggtg atggggggtg ggttatctgg aggtaagagg cgatccatcg
    10561 ggtagggcgt ttttgggagg cccccgtcac ctgtcctctt ccagcaggtt ctcgcgggtc
    10621 tccgggggga gggtcgcttc gggggagaga tcgaagtaag gctcgatgat cggatcgttc
    10681 tcgcaatcgt gcaactgctt cccttcaccc cgggtttgca tccagagcct gatctgctgg
    10741 agtaatgtca cgtggttcaa tcctttcgtc cgcagcgggt gagtcacccc tgtgacgtat
    10801 agccattctg atcgagcgag aataaatcca ttgtgatacg ccatttggct caaatagccc
    10861 tcgttctccc ctctatgcag gatccggaga ggaactgcag ggattcgtag tggacggctc
    10921 cgtcaccgcc cgaacatgcc gtcgagcctg cgcctatcaa acgccaccac cgtcggcctc
    10981 ccgtgcgggc acgtccaggg ggtctttgta cgggcgagct gtgagaggag gcgtctctgc
    11041 tggtcgtgcg tgagaagggt gccggccttc accgcccccc tgcaggcgac gatgcaggtg
    11101 accgcctccc gccggccggg ggtcgtccgg gactcgccgg cgagtatatc ggcgatcgtc
    11161 tccctgatgc tctccggatc ctccatagcg ccgagggcgg ccggcaccgc ccggacggcg
    11221 aaggtatccc ggccgaactc ctccacctca aacccctctt ccgcgaggaa cggcatcgcg
    11281 tcccggagcg ctgccgattc cttcgggggg agggagagga cgacgggcat gatcagctcc
    11341 tgcgacccgg gcgccgcatc gcgccgctcc gcgacctggt cgtagaggat ccgctcgtga
    11401 gccgcgtgct ggtcgatgag gtagagggtg ccgtcgaccc cctccgccac gatgtaggtc
    11461 gcggccacct gcccgaccgg ttccatggcg gggaggaggt tttcttcccc ctccccctcc
    11521 gtccggcgaa gctgcctatc ggagcgggcg atatcgcggt ggcctgcggc gtaggccgct
    11581 tcggcctcac gggctgccgg ggccgcctca ggtctctctc ccgtgaggac ctgctgcacc
    11641 ggctctgccg gcgtagcgcc tgcaaggtcg tgcccgagga gtgcctcttc taccgcggcc
    11701 gcgaccgccg cgaggatctc cctctcccgc gagaggcgga cctcgcgctt tgtcgggtgg
    11761 acgttgacgt ccacgagatc ggtatcgatc gcaaggtcga tgaacgccac cgggtagcgg
    11821 tccttcggga ggagggtgcc gtaaccctcc cggacggcgg cggcgatcgg tcgggaggag
    11881 atgctccggc cgttgacgct gatgacgacc tgtgacgggt tcccccggct ctccgacggg
    11941 cgggagacgt agccgccgat ggagaggaac ggaagccttc ccctgaccgg gacgagcgcc
    12001 cgggccaggt ccgcgccgta gagcccggcg atggtggcga taagctcgcc ggatcgctgg
    12061 accgccatcc gctccttccc gttgtgcacc acccggaagg caatccccct gtgcgcgagc
    12121 gagaggttct ccacgacgcc gtataggtga gcgagttcgg tgttcctgct cttcaagaac
    12181 ttccggcggg cgggggtgtt gtagaagagg tgctcgacgg cgatcgtcgt tccctccggc
    12241 gcccccacat cgcccgtctc cacgacctcg ccgccccgga cgacgacccg ggtgccggca
    12301 agcgccccgg cgccccgcgg ccgggtgacc agggtcacct ccgcgacggc ggcgatgctc
    12361 gcgagcgcct ccccccggaa cccgagcgtc ctgacggtag aaaggtcggc tatatcctgt
    12421 atcttgctcg ttgcgtgagg gtgaaacgcg aggagagcct cctttgcggt catgcccgca
    12481 ccgtcgtcgg tcacccggat cttcgtgacc cctgccatgt ccgacccgac atcgaggagg
    12541 atgctcgtcg cgcccgcgtc gatggcgttc tcgaggagct cctttgccac cgatgcgggc
    12601 cgctccacga cctcgccggc ggcgatctgg ttcacggtat cggggtcgag tatgcggatc
    12661 ttcgtcatcg ctccccttct ccgttcgcca tcctccggag gcggcagagg gcgttcaacg
    12721 cttcgatcgg cgtcatctca tcggggttga gcctcttgag ctccccgacc gccgggttct
    12781 cggcaacagc cccctccccg ggatcgacga tgagcatctg ggtgtagcgg ggtgcccgca
    12841 ccccggtcgg gagctcccgc accgacgcct ccttcaggag gtccatcgcc cggtcggtca
    12901 ccttcttcgg gagaccggcg aggcgggcca cgtggacgcc gtagctcctg tcggtcgcgc
    12961 cgggtatgat ctttcggagg aagacgacgt cctgtcccgt atccttcacg gcgaagtgga
    13021 agttcttcac cctcttctgc gacccctcaa ggtcgatcag gtcgtggaaa tgggtcgcaa
    13081 agagcgtccg ggggccggcg atagcccgcc cgtgcaggaa ctccaccacc gaccttgcta
    13141 tcgcgcaccc gtcgagcgtg ctcgtccccc gcccgatctc gtcgaggacg accaggctct
    13201 gcggcgttgc gttgttcaag atgtttgcga gttcgagcat ctccaccatg aacgtggact
    13261 gcccggaggc gagatcgtca aacgccccca cccgggtgaa gacccggtcg acgatgccga
    13321 tcgtcgcgtg gcgggcaggg acgaaacacc ccatctgggc catgatgcag cagagagcca
    13381 ccgcccgcat gtaggtggac ttgcccgcca tgttcgcccc ggtgatgatc atgatctggt
    13441 cgccggcgct atcgagttcc gtgtcgttcg ggacgaacgg gaccgggagg tgccgctcca
    13501 cgaccgggtg gcgcccgtcg cggatgacga tccggccgct ctcctcgata gccgggcggg
    13561 agtagccgta gcgggcggca acctcggcga gggccgagta gacgtcgagc agccccacgg
    13621 cacgggcggt cgcctggagg ccggggacct ccgccgcgag cgtccggacg agttctgcgt
    13681 agatctccgc ttcgagcgcg gaaagccgct cctccgcggt cgctatcagc gcctccttct
    13741 cccggaggtc gggtatcgtg tagcgttcgc cgttcgcggt cgtctgccgc cgctcgtact
    13801 ccggcggcac cagggggagg tttggtttcg tcacctcgat gtagtagcca aagacccggt
    13861 tgtaggcgac cttgagcgac ctgatgccgg tccgctcccg ctcctgctgc tggaactcca
    13921 cgatccagtc cttcccggtc gttgcaagac cccggagctc gtcgagtttc gcgttgaacc
    13981 cctcccggat catgccgccg gattttgcaa gcgccggggg gtcatcgacg atcgcccggc
    14041 cgatgaggtc gctcgcggag gcgtgatcgc tcattgcatc gagggcctcg cggacgaggg
    14101 tcggaaggtc gcccggaaga agcgtcttca cgcccgggac cgcagaaagc gattccctga
    14161 gcatcgcaag atcccggggg ccggcgttcc cgtaggcgat ccttcccgct atccgctcga
    14221 tatcggcaaa atcgccgaga gccgcgcgga gcgcctggcg ctcgacggtg tggccgatga
    14281 gccactcgac ggcatcgagc ctctcctcga tcgccgcctt cctgaccagg ggggctacca
    14341 ggaacgaccg catcgtccgg cttcccatcg acgtctccgt gacgtcgagg gcggcaagga
    14401 gggtgctcct gtcgccttcg ccccggatgc cggtcgtgat ctcgaggttt cggagggtga
    14461 tcgcgtcgag caccatggtc cccgacggga cccgggtcga gagccccgtg atgtggggga
    14521 ggggcgactg ctgggtctcg cgggcgtaat ggagagccgc ccctgccgcg gcaacggctc
    14581 ccgtcatctg agcgcacccg tagccgtcga gcgtcgccgt cccgaactgc tcgcagagga
    14641 gcctgcaggc tgcatccgga tcgaacgcat cgtcgcggta acggctcacc gtcaccccga
    14701 tgctctcgag ccggccggcg agcccttcat cgagagcctc ggggaggatc gcctcggatg
    14761 gccggtacct gaccgcctcc gatacgacgt cggcgtactc gcgcccgccg ctccccgcgg
    14821 agacgaagaa ttcgccggtg gagacgtcca ggaacgcaag gccgaaggtg tcttttctgt
    14881 cgggggcgac cgccatcagg taccgcgccc cggccgagcc gagcatcgac gagtcgatca
    14941 gggttcccgg ggtgatcacc cgcgtcacct cccgcttcac gacgcccttt gcggtctttg
    15001 gatcctccac ctggtcgcag atcaccacct tgtagcccct gcccaccagg cgggcgatgt
    15061 agccgtcggc ggcgtggtgg gggacgcccg caagcggcat ccggtcgcct ttcctgtcct
    15121 ttccccgtgc cgtcagggtg atatcgagct cgcgggccac cacgccggcg tcttcaccga
    15181 atgtctcgta gaagtcgccc atccggaaga agatgatggc gtcgggatac cggttcttca
    15241 ccgagtagta ctgccgcatc gccggggtcg gtccatccgt cattgcttct ccctatacaa
    15301 tccgcgcgag gccacataag gatactcctt gagaaatatc gccgaatacg gccccgatta
    15361 cctgcaccac gaggccggac cccgcaaagg tagatacgtc cggagcgcgt accaggaaca
    15421 cgataactca tggcggcgac gatcgcagag aagatattct cgatgaagtg cggcagatcc
    15481 gtccgcgcgg gggacgtcgt gatggccccg ctggatgcgg cgatgatcca cgacatcacc
    15541 gggccccttg cggttcgggt ctttggtgag atgggaggag aacgagtctt tgatccggag
    15601 cggatcatca tgctcttcga ccaccaggtt cccgccgact cgataccggc tgccgagaac
    15661 cacgtcttca tgcggcggtt tgcagaggag caggggatcc acaactacga cctcctcgag
    15721 ggcgtctgcc accaggtcgt tatggagaag gggcgggcgg cgccgggcga gatcatcgtc
    15781 gggaccgact cccacacctg cacctacggt gcggcgggag cgtttgcgac cggcatcggc
    15841 tcgaccgaca tggggttcgt cctgaagttc ggggcgctct acttccgggt gcccgagagc
    15901 atcctccttg aggttgacgg ggtgttcgac cggcgcgtgg gggcaaaaga cctgattctc
    15961 tcccttgccg gggatatcgg cgccgacggc gcgacctaca tgacgcttga gttcaccggt
    16021 aaagcgatgg agaggatgaa tatggcgggc cggatgacct gcgcgaacat ggcgatcgag
    16081 atgggcgcaa aggcgggtat cgtccggccc gacgcggcca cgtgggagta tgtcgccgcc
    16141 cgccgcgaga tcgagccgtt cgatctcgcg ggcgacgatg atgcggtcta tcgcgagcgc
    16201 cggcactacg acgtcaccga cctcgctcca caggtcgccg tcccccacaa cgtcgacaac
    16261 gtcgtggatg tcgcggatgt cgcggggacg aaggtcgacc aggtcttcat aggttcctgc
    16321 acgaacgggc gctacgagga tctcgccgag gcgggggagg tgatggggaa cgccgaccgt
    16381 ttcgcaaagg gtgttcgggt gatcgtcata ccggcatcgc ggacggagta tctcaaggct
    16441 ctccgggccg gtctcatcga gcggttcgtc gaggccgggg ccctcgtgga agcgccgtgc
    16501 tgcggcccct gcatgggggg tgcgttcggg ctgcttgccc cgggcgaggt ctcgctctcg
    16561 acgtcgaacc ggaacttccg gggcaggcag gggagcgctc aggcctcggt ctacctctcg
    16621 tcgccggcga cggcgatggc aagcgccctc tacggcgaga tcaccgatcc gagggaggtg
    16681 tgataccatg cgagtctgga agttcggcga cgatatcgat acggatgcga tcataccggg
    16741 gaggttcctc acgatctacg atccggcgga actcgcgaag cacgcgttcg aggggacgag
    16801 ggatgagttt gcgcgcgatg tgcgggaggg cgacgtcgtc gtcggcggca ccaacttcgg
    16861 gtgcggctcc tcccgcgagc acgcgccgct tgcgctcctc ggcgccgggg tccgtgtcgt
    16921 ggtcgcgaga tcgtttgccc gtatattcta ccgaaacgcc gtcaacacgg ggcttttgcc
    16981 cctggtcgct gccggggcgg acagcatccc ggacggtgcc gccgtatcgg tggatatcgc
    17041 cggcgggttt gtcgaggtgg acggcagaag gctcgtcgtc gagacggtgc ccgggttctt
    17101 gaaagagatc gtcgatgccg gcgggcttgt cgcctacgcg aagaacctcg acgaggtgaa
    17161 gacgtgcagc acagagtagc ggcgataggc ggggacggca tcggtcccga gatcgtcgcc
    17221 gcaggaagag aggttatcga cgccgcaggg gagcggtatg ggtttgacat cgcctggacc
    17281 gacttcgacg tcggggcgga gcgttacctt gcgaccggag aactcctcac cgaggaagat
    17341 atccgggagc tctcgaagtt caaggcgatc tacttcggcg ccatcggcga cgagcgggtg
    17401 aaacccggga tactcgagaa gggcattttg ctcgccatcc ggttccactt cgaccagttc
    17461 gtcaacctcc ggccgatccg gcttcttaag ggagtcgcga caccgcttgc acacaagcgc
    17521 ccggaggata tcgacttcgt cgtcgtccgg gagaacaccg aggacttcta cgtcggcatc
    17581 ggctcgcggt tcacggggaa gcgcgagagt aagaccctcg aggtcgtccg cgacctctac
    17641 aacgtcaaat tcggcctcga cgtcgagacc gacgccgacg aactcgccta ccagataggc
    17701 gtcgtcaccc ggccgggcgc ggagcgggtg atccgctacg ccttcgacct tgccgggcgg
    17761 cgccgcggga aactcacgtc ggtcgacaag gcaaacgtcc tctccgacgt ctacggcctc
    17821 tggcgggacg tcttcacggg cgtcgccgcc ggctaccccg atgtccagac ggagttcaac
    17881 ttcgtcgatg ccgtcacgat gtggttcgtc aagaaccccg agtggttcga cgtcgtcgtc
    17941 accccgaaca tgttcggcga catcatcacc gacctcgggg cgatgatcca gggcggtctc
    18001 ggccttgccc ccggcgggaa catcaacccc gcaggtacat cgatgttcga gccgatccac
    18061 ggttcggcgc cgaagtaccg cggccaggac gtcgcaaatc cccttgccac catatgggcg
    18121 ggctcgatgc tcctcgacca catcggcgaa cacgaggcgg cgaaggcggt tctttcggcg
    18181 gtcgagcaaa gcatcctcga cgggttcgtg acccgggata tgggcggagc aaagaagacg
    18241 agcgaggtcg gcaggtacgt cgcctcgctc gtccgggggg tgtagggcgg ccggcccctt
    18301 ctcccgcact cccatggtcc tgccgatgcc agaaaaccag catacgccgt gcgagaaccc
    18361 cctctggccc tgtgcgatga ccggggcggt cgcctgcctt gcggggttcg aggacctcgc
    18421 cgtcgtcgtc cacggctcga gcgggtgcta cttctacccc gcatccctgg tcggggttcc
    18481 cgtccacggg acgttcctca tcgagaacga gatcatcttc gggacggagt cccgccttgc
    18541 ggaggtgatc cacgaccttg aggggcgcta ctcgcggata gccgtcgtga acacctgcgt
    18601 gcccgcgatc atgggcgagg atatcggcgc cctcgcccgc gacggaaggg tgctgatcgt
    18661 cgacagcccg gggttcatcg gcgacctcga ggccggctac cgccgtgcgc tcgatgcgct
    18721 cgcgcccgag gtggacgagg gcatcgccgg cgtcaacatc gacggcatct gccgcaccga
    18781 ccctttctgc cgggggaacg ccatcgaggc gcgacgcctg ctcgaccttg ccggcgcctc
    18841 agtcggcacc accttctgcc tggaccgctt cgatgcggcc caccgtgcgg cgcccttcac
    18901 cgtcgggacg aaccccgatc ttgcaagcgg cgtcggaacc gcctgcggct cgctcctcgg
    18961 gcttgatgcc gtcagggaga ccttcgagag gcttggtgcc gttatcggcg ggatcgatac
    19021 ccggccggtc gcagaggagg tcgcgtgggc cgatgagcgg atccggaagg cctgtgagaa
    19081 gtacctccgc aggttcgatc ctccccgggc cgcgatcgcc gcccagagcg cgtatgccgc
    19141 ctttgccgcc gatctcctcg accgctacct cggcgccgac atcgcctgcg tcgcggcccg
    19201 aaacgatccg ggggaggggg gcgccgccgc tgtccgcacg accgactttg cggttctcca
    19261 ggagaggatg acgggcgccg agccggacct ggtcatcggc tcctcctacg agcgggcgat
    19321 ggctccgggt gccgcgttcg tcggcctgac gccgcc
//
LOCUS       DAOSII010000023        18987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_3138_length_18987_cov_55.252588, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000023 DAOSII010000000
VERSION     DAOSII010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 18987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18987
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3138_length_18987_cov_55.252588"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..1310
                     /locus_tag="PLV90_03125"
     CDS             102..1310
                     /locus_tag="PLV90_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOF96138.1"
                     /translation="MKIIKDPVHGYVEADEPMLRLLDSGVVQRLRHVTQLGFANLVYP
                     GANHTRFEHSLGAMHLAGIMCRELGLDGEETRLVTTAALLHDVGHGPFSHVTEPVMEE
                     FVGRKHHQIGYLVREGAIAAILEAEGIDPAEVCAVVAGEHRFASIIHGSLDVDRMDYL
                     MRDAHYTGVPYGTVDAHRLIRCSVLAESGIALRESGINAAESLLIARTLMRPAVYFHH
                     VSRIATSMFVHALREEVLTVPGANARELVRMDDAACMERLKHSPSPIVRDLASRVYAR
                     DLYKRALYVGEDRVNAAALRQDPGPAREREIARDIAGVAGVPVAKVLVDIPPLPRALS
                     MEVRVRNGHAMVDIEEVSPLIGTLNDTRRQQWRLGVYTAPEHRQAVEQAATEVLRVKR
                     ATKQDKLVVT"
     gene            1325..1879
                     /locus_tag="PLV90_03130"
     CDS             1325..1879
                     /locus_tag="PLV90_03130"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HOF96139.1"
                     /translation="MKKEFVIGVTGASGVIYARRLLEVLCDRATVHIVVSDTARQIAG
                     IEGVDLTGFDAVYAENENLAADIASGSFRYDGMAIVPCSMKTLAAVSNGLADNLIARA
                     ADVCLKERRPLLLLLREMPLSRVHLRNMLAADEAGATVMVASPPFYQRPATIDDLVDM
                     VVARVLDHLGVEHALGSRWSGYDA"
     gene            1872..3113
                     /locus_tag="PLV90_03135"
     CDS             1872..3113
                     /locus_tag="PLV90_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HOF96140.1"
                     /translation="MRDFIAMMREQGRVEDIERPCSTIYEAPRMASRTDKILFFHDLD
                     GHRAVMNLLGDRKSLAAALGVGERDLVRNLAARTYNGRVVDAGRLAGGIPADLSRLPV
                     MKHFPGDAGRYFTAGVVFSRYGGVENASIHRMMVLDDHRVVARLVEGRHTHTLLKAAL
                     AGGERLPVAVTVGTHPLVTFAACTRVPEGKELPYAAELMGGELAVHECANGVRVPGAE
                     IVLEGSIGPETAAEGPFVDITGTYDPVRQQPVIEFSRMYLRENPIYHGILPAGDEHKL
                     LMGAPYEPKIYRAVGEVTTVRDVLLTKGGAGYLHGVVQIRKNTDGDAKNAIMAAFAAH
                     TSLKHVVVVDEDIDIHDPYDVEYAIATRVRGDTDIMIITGVRGSSLDPMRLADGTNVK
                     VGVDATMLMGREGEFRRAEWP"
     gene            3117..4277
                     /locus_tag="PLV90_03140"
     CDS             3117..4277
                     /locus_tag="PLV90_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HOF96141.1"
                     /translation="MYLNEDDERILAGEFGETRQKMLEILVALGNVYGAERLVPITSA
                     QVSGASYKTIGRWGLAWLQSLNARVAVPAVLNPIGMPREEWREFGIREEFAHNQGAVI
                     EAYRRLGIKLECTCTPYYLRITEYGEHLAWSESSAVAYANSVLGARTNREGGPSALAA
                     AIIGKTPYYGLHIVANRRPQVVVDVEGGTGLRSDHWGAIGHVAGRKVGNRIPIFAGIR
                     PNRDQLKALGAAMAATGAVALFHVDTITPEARVFSFDTGDLDRVTVSADEVETLFAEM
                     DVEAVAVGCPHCSAEELRELADLLRGKKTTKPFYIFAARGVAKNNPDLIAAIERSGAR
                     VIPDTCMVVSPRMDEFSSIMVDSGKALAYVPGMCGALARIGTRRECVEVATS"
     gene            complement(4389..5075)
                     /locus_tag="PLV90_03145"
     CDS             complement(4389..5075)
                     /locus_tag="PLV90_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96142.1"
                     /translation="MRRELLLCGILASILIVACAPAVTAAAVGPVMPRDPVGPTASRM
                     GNVIKPGTVLTYDASISRITTDQYGMSSQSYVTGFDWVIRVEEASGGRYAGTAEIVPR
                     SAGMTGGVYRWSYVEGQQNPDWYGPPLWVDPGAPAESAGTYGSSTWQLLGDPRGLRMP
                     NPSTGVEQSVNQMQTVGPGVLRYISYDTDYGTVLLYSEKTDQYFMSFWFRSLNEGGGG
                     LTGPFGGMVS"
     gene            5417..5686
                     /locus_tag="PLV90_03150"
     CDS             5417..5686
                     /locus_tag="PLV90_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOF96143.1"
                     /translation="MEEKDIDTLRRRRDIDGLIRALSDPAGIVRLTAAEALGSAGDER
                     ALEPLERLKFSDPDIGVRRAASLAHARVAARLAEKKAVEGWLLDR"
     gene            complement(5714..6805)
                     /locus_tag="PLV90_03155"
     CDS             complement(5714..6805)
                     /locus_tag="PLV90_03155"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HOF96144.1"
                     /translation="MEYPPPKPVSKAGPFRDIPVDILRGLAITFMIMANLTPALLLPP
                     APGWYRFLSSLAAPIFIGLAGMMVALSRTGKGRPFSYIATRGGLFILAGALLQVLIFD
                     YVPFLDVDVLYLIGLALPLTYLYLGLPERGRWAVIVAILCLTPLLQVGLGYSGAVITR
                     PDVTLTPFSISGDPFPAPVEIARAWLMEGWFPVFPWLAIAFFGAELGKYRWRDRAAPR
                     FRFKDEGRWGAGLLLAGAVLWMLFPGAQYIRDSYVELFYPPMIGFFVFAAGLVVFALT
                     VLDHIPAQYGLLDPLRAAGGCSLAIYLAQYTVIVKVIEPFGLQLPLPAYLGVFLVLYT
                     GMILLAYFLRYVRTSWHGQPFLVRFLIGS"
     gene            7030..7308
                     /locus_tag="PLV90_03160"
     CDS             7030..7308
                     /locus_tag="PLV90_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOF96145.1"
                     /translation="MTAPEDIDAMRDARDVAGLIRALDDADEFVRAQAALSLGTIADP
                     EAREPLDRMRREDPSVSAREAAAIAYKWLVGRLEEVEAVRGPKPPPPW"
     gene            complement(7316..8596)
                     /locus_tag="PLV90_03165"
     CDS             complement(7316..8596)
                     /locus_tag="PLV90_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HOF96146.1"
                     /translation="MIGPELIAIAIFLFTYALIIDERIHRAVAAMLGASVIVFLHIVP
                     WEKIPVYVDLGTIFLLMGMMIIVNTARGSGLFEYIAIKTAKLAKGSPMRVLLLFSLVT
                     AVISAFLDNVTTVLLITPMLLYIAGVMRITPLPFLIAEIFASNVGGAATLIGDPPNIM
                     IGSAAGLTFNEFLVNLAPIMVVNIVVVMGMLALIYRKELHVSPGEKGVIEKTFAELNE
                     QEAIRDISLFKKSVAVIALVIGMFFIHDLLGLEPALVALIGASILLFWSRQPPEEIFE
                     KIEWPALFFFGGLFIVVGALVETGTIAAVAGFVVESVHSEGEAMLIIAWFSAIASAVV
                     DNIPLTATLIPLIHDMGASMNTYPLWWALSLGACLGGNGTAIGASANVVVLGIAARNG
                     LTISFVEFLKVGMLVLFVTVGIGTAILWLNFFVL"
     gene            complement(8793..11504)
                     /locus_tag="PLV90_03170"
     CDS             complement(8793..11504)
                     /locus_tag="PLV90_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOF96147.1"
                     /translation="MIVPTGDREEALPAEWYTLSSDEVEQTLGTGPHGLSREEAEARL
                     QRYGANVLREEERETRLQVFLRQFASILIVILIVAAVVSFFVGEPQDAAAILIIVVLN
                     ALLGYSQEWQAGEAIEALRQMLVQKAVVIRDGKQQEIDASGIVPGDLVALDMGDRVPA
                     DLSLVEATSLQVDEAPLTGESSPVDKATGPLTPETPLAERSNMVFAGTTVVNGWGRGV
                     AVATGMRTEFGLIAGLSQRVRAEATPLARQMDVLGRDIGIIAVGIAVLAIGVGFLQQR
                     EFLEMFLIGVSLAVAVIPEGLPAVVTLTLAIGIKNMMRRNCLIRRLPASETLGAVSVI
                     CTDKTGTLTRNEMTVVRVRTPESEVAVTGTGYAPEGEFLRDGSAIDPLADPDLRQFLR
                     AALLCNHATFAGDEAGEWRIFGTPTEGALVVAAAKAGLSRDDAPEAVREFSFNSTRKR
                     MTIIYREGDGDVAYVKGAPEVLLDLSSRLFRDGAVVPLTDDLRDALQQELCGYASRGL
                     RVLGAAYRPLPGDVPRTAEAVEKDLIFLGFAGIVDPPRPEAAEAIRLCRSAGIDVIMI
                     TGDNPLTASAVAQDLGLSSEGALTGADLEAMTDDELARRLRTTKVLSRVTAEHKLRVI
                     DILSRDREVIAMTGDGVNDAPALKKASIGIAMGIKGTDVAKESSDMVLVDDNFASIVA
                     GVEEGRREYDNIARFTRYLLSSNVGEIVAIVGGLLMGLPLILIPVQILWINLITDGPT
                     ALALGLEPAEKDVMQRRPRNPDESILTRSALLVILVIGAWLGLLTLYAFSGFYEVDID
                     RARTMAFTGLIIFEMYNVLNFRSFRFPLHRIGFFSNPTLILAILGSLGLQALVVYVPI
                     LQEFLGTAPLTLADWGLLFLLGLPILLAGEIYKILRLRSEEKGRTPA"
     gene            11650..12201
                     /locus_tag="PLV90_03175"
     CDS             11650..12201
                     /locus_tag="PLV90_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HOF96148.1"
                     /translation="MSRIAVLITDMFEDVEYTEPAAAFREAGHDLVHVGLSAGETVHG
                     KKGEAEVTIDRAVSDVSVDSFDALFIPGGYSPDKLRAHDAPVKFVQEFVQSGKPVLLI
                     CHAPQLLITAQALSGRRITGWRSVAQDIKNAGAEYVDQEVVVDGNLVSSRQPDDIPAF
                     VRASLEKLAEAGGKVEAAAVRQR"
     gene            12269..13468
                     /locus_tag="PLV90_03180"
     CDS             12269..13468
                     /locus_tag="PLV90_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOF96149.1"
                     /translation="MKPAGSTWSRPAVIVLTAVLIVLLGAIARVLTGIDAAYTDLLYL
                     VLIPTAYWYRRHAVYPGILVAVLHIVATYIVTGHVGAETLVRAGMLIVIPYVLGYFFE
                     VVGRRTEKKHFHAGESGPVACDRSTKRLIARLSSRDPDTRYEAAGCLGNAGDPAAVLP
                     LAALLRDPESGVRWKAAEALGRLGSPAVEPLSGSLQSEDVDVRWMAAVALGDIGDPAA
                     VPALAKALDDDDAYVRSRAALALAAIGEPAREMLITGLSTGNARVRGSAALALGRLGG
                     ESATGALIDALRDPDEEVRHRAAVALGDIGEPAVPDLLSATRADEADVRRGVTAAFGQ
                     IGKPAVAPLIAALDDDDWRIRRGAAAALGDIGDPGSVDALIRALDDAREEVREQARKA
                     LGSIRKT"
     gene            13495..14016
                     /locus_tag="PLV90_03185"
     CDS             13495..14016
                     /locus_tag="PLV90_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOF96150.1"
                     /translation="MTDETRIDELVADLRRGPLAVRRAATAELGASGAVAVEPLIAAM
                     LDGDNDLRWYAARALVQIGEAAIDPLLAAMRAGENRDFRRYAMAALAGIGEAAVEPLI
                     DIIECADPALQPFAAMALMKIGEPAVEPLLRLMQSPDPKTQERAALLLCRMGETGAEP
                     LTGALEADRSGKR"
     gene            14199..18062
                     /locus_tag="PLV90_03190"
     CDS             14199..18062
                     /locus_tag="PLV90_03190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase II large subunit"
                     /protein_id="HOF96151.1"
                     /translation="MEVSPAIERYFENLAAGLDSAMALAAAARARGLDPRTEIEIPVA
                     SDLADRVEALLGYPGIAARIRELEAEMSREEAALRIGDDFAARMFGETTTEEILDHAI
                     RGAMALLTEGVVAAPTEGIAKVSLGKNDDGTDYLKIYYAGPIRSAGGTAQALSVLVGD
                     YVRQALGINRYVPRPEEVERYIEEIRQYNNIMSLQYLPSEKELRTIITNCPVCIDGEP
                     TEQQEVSGYRNLERVETNTVRGGMALVVAEGLALKAPKIVKNVKKMKMAGWDWLEEMI
                     GGGGAAKSDDDDKGAAVKPKDKYLRDLIGGRPVFSYPMRKGGFRLRLGRSRNTGFAAA
                     GLNPATMHILGDFLAVGTQMKIERPGKAAGIVPVDSIQGPTVKLRSGEVRRVDDAAEA
                     RRIAGQVDEILDDGEILISFGEFMENNHPLMPPCYCEEWWRLEGGPRHPASELEAIEF
                     ALDGIPLHPDYTYLWDDVAPADIALLADRISAGGRIEGGVLTLPDTPEAKAILEELLV
                     PHRLSGDRIAIPGYLVLLACLGLTLHLDKRPAWENAPLENSLDLVMHLSGFSIRSRAG
                     TRVGGRMGRPGKSKQREMKPPPHSLFPIGDEGGSRRSFQAACASRPRSNTDGGVIEVE
                     VGERRCPSCGTFTYKNLCDCGAHTRPVLRCPKCNQEIGKDMCPRCSVATVCLQKVSIN
                     VRIEYAAALANLGVRESAIPLLKGVKGLISRERPVEPIEKGILRALQNLYVFKDGTVR
                     YDMIDLPLTHFRPDEIGVPVERLRELGYTHDIYGRELVETDQVLELRHQDILVSEDCG
                     EWLVRVAKFIDDLLVRVYGLEPFYRAEQPLDLIGHLLMGLAPHTSAGVLVRLIGFSKA
                     GVGYGHPFFHAAKRRNCFAGDTEIAVSDGRQWITVPIRQFVVENFDLSKPGLDRTGTF
                     YSDPRQPFYARSIDPQGLVGVKKITSVSIHRAPAHLIRFVTGRGKVLSVTPDHAMLVW
                     DTDYLRKIRALEVKIGDRVPVEEGGLVISDVVVSRETVQALDDRVYCLTVAENHTLVA
                     NGIFCGQCDGDEDCVMLLLDGLINFSRAYLPETRGGTMDAPLVLTTRIDPAEVDKESL
                     NVDVCDHYPLEVYNGCLAYAHPKDLDKYVDRVERRLGTPAQVEGFFFTHPTSDISAGP
                     LESTYTKLGTMLEKLDAELDLAKKIRAVDADDVAERVLNTHFIRDLQGNLNAFSKQKV
                     RCMKCNAKYRRMPLAGKCTRCGGNVIPTVHEGSVKKYLDMSRNICETYAITEYTKQRV
                     EVLFMQIESTFGEPPERQLGLADFM"
     gene            18069..18896
                     /locus_tag="PLV90_03195"
     CDS             18069..18896
                     /locus_tag="PLV90_03195"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOF96152.1"
                     /translation="MEAREVLSAIRAGIPIRGDRRRPEIEPLPGIRAYRLTTPATRAD
                     RTILFFHAGGFTSGSTADSFDLCARLCDAARSEVVSVDYRLAPAHPFPAAVEDCLSAY
                     RYLLREGYSPERVVPAGLSAGGTLALSMLLSARGAGLPMPAAGVLLSPAVDMLFLGES
                     VLLNRETDWLAPEQFAAMREDYLAGGDPCDPLASPLYADLSGLPPLLVQAGGGEVLID
                     GIEAFVSTARLQGVPVAFDCTEGMFHCWQAFAGVLPEGAMAVERIGAFVRRHVPDEG"
BASE COUNT         3450 a         5971 c         6432 g         3134 t
ORIGIN      
        1 gggcgccccg gatgagcgcg ggcgtcccgg ttccgccgga gaggaaggtg atcattctcc
       61 atacctttat cactcctctc ccaagaacct taacggaacg gatgaagatc atcaaggatc
      121 cggtgcacgg ctacgtcgag gcggacgaac cgatgctcag gctgctcgac tcgggagtcg
      181 tccagaggct tcgccacgtc acgcaactcg ggtttgcaaa cctcgtctac ccgggcgcaa
      241 accacacccg gtttgagcac tcgctcgggg cgatgcacct tgccggcatt atgtgccggg
      301 agctcgggct tgacggcgag gagacgcggc tcgtcacgac ggccgccctc ctccacgacg
      361 tcggtcacgg gcccttctcc cacgtcaccg aaccggtgat ggaagagttc gtcgggagga
      421 agcaccacca gatcggctac ctggtcaggg agggagcgat cgccgccatc ctcgaggcgg
      481 aggggatcga tcccgccgag gtctgcgccg tcgtcgccgg tgagcaccgc ttcgcgagca
      541 tcatccacgg gagcctcgac gtcgaccgga tggattacct gatgcgggac gcccattaca
      601 ccggcgtgcc ctacgggacg gtcgacgccc accgcctgat ccggtgttcg gtcctcgccg
      661 aatcaggcat cgcgctccgc gagagcggca tcaacgccgc cgaatcgctc ctcatcgccc
      721 ggaccctgat gcgccccgcg gtctacttcc accacgtgag ccggatagcg acgagcatgt
      781 tcgtccacgc cctccgcgag gaggtgctca ccgtcccggg cgcgaacgcc cgcgaactcg
      841 tgcggatgga cgacgccgcc tgcatggagc gtctgaagca ctcgccctct ccgatcgtcc
      901 gcgaccttgc cagccgggtc tacgcccggg acctctacaa gcgggcgctc tacgtcggcg
      961 aagaccgggt gaacgccgcg gccctccggc aggaccccgg gcctgcccgg gagcgggaga
     1021 tcgctcgcga tatcgccggg gttgccggag tcccggtggc aaaggtcctc gtggatatcc
     1081 ccccgctccc gcgggcgctc tcgatggagg tccgggtcag gaacggccat gccatggtgg
     1141 atatcgagga ggtctcgccc ctcatcggca ccttaaacga cacccggcgg cagcagtggc
     1201 gcctcggggt ttacaccgcc ccggagcacc ggcaggcggt ggagcaggcg gcgaccgagg
     1261 tccttcgggt gaagcgggcg acaaaacagg ataaactcgt agtgacataa tatatgagga
     1321 agagatgaag aaggagtttg tcatcggggt caccggggcg agcggggtca tatacgctcg
     1381 ccgcctgctc gaggttctct gcgatcgtgc aacggtgcac atcgtcgtat ccgacacagc
     1441 ccggcagatc gccgggatcg agggcgtgga ccttaccggg tttgacgccg tatacgccga
     1501 gaacgagaac ctcgcggcgg atatcgcgag cgggtcgttt cgctacgacg gcatggcgat
     1561 cgtcccctgc agcatgaaga cgcttgccgc ggtcagcaac ggccttgccg ataacctgat
     1621 cgcccgggca gcggatgtct gcctcaagga gcggcggccc ctcctcctcc tcctccggga
     1681 gatgccgctc tcccgggtcc acctgaggaa catgctcgcc gccgacgagg ccggcgcgac
     1741 cgtgatggtc gcaagccctc ccttctacca gcgcccggca acgatcgacg acctcgtcga
     1801 tatggtggtg gcccgggtgc tcgaccacct gggggtcgaa catgctctcg ggtcccgatg
     1861 gagcggatac gatgcgtgat ttcatagcga tgatgcggga gcagggccgg gtcgaggata
     1921 tcgagcggcc ctgctcgacc atctacgagg ctccccgcat ggcgagccgg accgacaaaa
     1981 tactcttctt ccacgacctt gacggccacc gggcggtgat gaacctgctc ggcgaccgaa
     2041 aatcgcttgc ggcagccctc ggcgtgggcg agcgcgacct cgtccggaac ctcgcggcca
     2101 ggacctacaa cggccgcgtc gtggatgccg gacggctcgc gggcggcatc cccgccgacc
     2161 tctcccgcct cccggtcatg aagcacttcc cgggcgatgc cgggcgctac ttcacggctg
     2221 gcgtggtctt ctcccgctac ggcggcgtgg agaacgcctc catccaccgg atgatggtcc
     2281 ttgacgacca ccgggtcgtc gcccgcctgg tggaggggag gcacacccac acgctcctca
     2341 aggcggcgct tgccgggggg gaacggctcc cggtcgcggt caccgtcggg acccatcccc
     2401 tcgtgacgtt tgccgcctgc acccgtgtcc cggaaggaaa ggaactcccc tacgcggccg
     2461 agctgatggg cggagaactc gccgtccacg agtgtgcaaa cggcgtccgg gtccccggcg
     2521 ccgagatcgt gcttgaaggc tccatcgggc cggagacggc ggcggagggg ccgttcgtcg
     2581 acatcaccgg gacatacgac cccgtgcggc agcaaccggt gatcgagttc tcccggatgt
     2641 acctcaggga gaaccccatc taccacggca tcctccccgc cggcgacgag cacaaactcc
     2701 ttatgggagc cccgtatgag ccgaagatct accgcgcggt cggcgaggtg acgacggtcc
     2761 gcgacgtcct cctcacgaag ggcggggccg gctacctcca cggcgtggtc cagatccgga
     2821 agaacaccga tggtgatgca aagaacgcca tcatggcggc gtttgccgcc catacgtcct
     2881 taaaacacgt cgtcgtggtg gacgaggata tcgatatcca tgacccctac gacgtcgaat
     2941 atgcgattgc cacaagggtg cggggcgata ccgacatcat gatcatcacc ggcgtgcggg
     3001 gttcgtcgct tgacccgatg cgccttgcag acgggacgaa cgtgaaggtc ggggtcgacg
     3061 ccacgatgct tatgggtaga gagggcgaat tcaggagagc ggagtggccg tagttcatgt
     3121 acctgaatga agatgacgag aggatcctcg ccggcgagtt cggcgagacg cggcagaaga
     3181 tgctggagat cctggtcgcg ctcgggaacg tctatggagc cgagaggctc gtcccgatca
     3241 cgagcgccca ggtgagcggt gcgtcgtata agaccatcgg gaggtggggg cttgcctggc
     3301 tgcagagcct caacgcccgg gttgcggtcc cggccgtctt aaaccccatc ggcatgccgc
     3361 gggaggagtg gcgggagttc ggcatcagag aggagtttgc ccacaaccag ggggcggtca
     3421 tcgaggccta ccggaggctc gggatcaaac tcgagtgcac ctgcaccccc tactacctcc
     3481 gcataacgga gtacggggag catctggcct ggtcggagtc ttcggcggtc gcctacgcaa
     3541 actcggttct cggcgcccgg acgaaccggg agggcgggcc gagcgccctt gccgcggcga
     3601 tcatcgggaa gactccttac tacggccttc acatcgtcgc gaaccggcgg ccgcaggtcg
     3661 tcgtcgatgt cgagggcggc acgggtctgc gctccgacca ctggggcgcc atcggccacg
     3721 tggcgggcag gaaggtgggg aaccggatac cgatctttgc cggcatccgg ccgaaccgcg
     3781 accagttgaa ggcgctcggg gcggctatgg cggcgaccgg cgcggttgcg ctcttccacg
     3841 tcgacacgat cacgccggag gcccgggtct tttccttcga taccggcgac cttgaccggg
     3901 tgacggtgag cgccgatgag gtcgagaccc tcttcgcgga gatggatgtc gaggcggtcg
     3961 cggtcgggtg cccgcactgc tcggccgaag agctccgcga acttgccgat ctcctcaggg
     4021 gaaagaagac gacaaaaccc ttctacatct tcgctgcccg gggggtcgca aagaacaacc
     4081 ccgacctcat cgccgccatc gagcggagcg gcgcccgggt gatcccggat acctgcatgg
     4141 tcgtctcgcc ccggatggat gagttcagct cgatcatggt ggattcgggc aaagctctcg
     4201 cctatgtccc cggtatgtgc ggggcgctcg cccggatagg gacgcggaga gagtgcgtcg
     4261 aggtcgcgac gtcgtgagga gggccggcgc tccgtgccgc cctccccccc cgcagaccgc
     4321 atgccgcctt tcgcggggtc gtcgggggtg tctgcgatcc gccgcaggcg ccgggatggc
     4381 ccggtgcgtt acgagaccat ccctccgaat ggcccggtca ggccgccccc gccctcattc
     4441 agagatcgga accagaacga catgaaatac tggtctgtct tctcgctgta caggagaacc
     4501 gtcccgtaat cggtatcgta cgatatatac cgcagaaccc cggggcccac cgtctgcatc
     4561 tggttcacgg actgttcaac gccggtagaa gggttgggca tcctcaggcc ccggggatct
     4621 ccgagcagtt gccaggtgct gctcccgtat gtccccgcgg actccgccgg agctccgggg
     4681 tcaacccaga gcggagggcc ataccagtca gggttctgct gcccctccac atacgaccat
     4741 cggtacaccc ccccggtcat acccgcgcta cgcgggacga tctctgcggt ccccgcgtag
     4801 cggccaccgg atgcctcctc gacacgtata acccagtcga accccgtgac ataggattga
     4861 ctcgacatgc cgtactgatc ggttgtaatc cgggagatgg atgcgtcata cgtcagaaca
     4921 gtcccgggct tgatgacatt ccccatcctt gaagcagtgg ggcccaccgg atctctcggc
     4981 atcaccggtc ccaccgccgc cgccgtcacg gcgggtgcgc acgcgacgat aagaatgctc
     5041 gcgaggatcc cacacaacag caattccctt cgcatcagtc tcacatcctg caatcatcgt
     5101 tccacaccgg gtcatcctca ccgcgtccct ggtgccgcgc ggtggagcac catcccggcc
     5161 tctggaatgc gagcagtcga tgcgaaccgg gtatatagac ctatgcgctc ccggccatgc
     5221 cggcacgaga tctccgcgaa gtcgtcgccc ggccagtacc gatgaccacc acgacatcgg
     5281 gagcatcgtg cgggtttcac cggccgttac cgggcgggca ggggtccggg ttgccgtcgt
     5341 gccgggatcg cctccggaac actgatgaga tggcgggcca taactgcacc gtaaagactg
     5401 gtcgaggaga tcctggatgg aagagaagga tatcgatact ctgcggaggc gccgggatat
     5461 cgacgggttg atccgggcgc tctcggatcc cgccgggatc gtgcggttga ctgcggccga
     5521 ggccctcggt tccgccggcg atgagagggc gcttgagccg cttgagcgac tgaagttctc
     5581 ggacccggat ataggggtcc ggcgggctgc gtcgcttgcc cacgcccggg tggcggcaag
     5641 gcttgcggag aagaaggcgg tggaaggctg gctccttgat aggtaggccc gccagcacac
     5701 cggggagatg gggtcaactc ccgatcagga accggacaag gaacggctgc ccgtgccagg
     5761 atgtcctgac gtaccgcagg aagtacgcaa gcaggatcat gccggtatag agaaccagga
     5821 agacgcccag gtacgccggc aacggcaact gaagtccgaa gggctcgatg acttttacga
     5881 tgaccgtata ctgggcgagg tagatggcaa gcgagcaccc gccggccgcc cgcagggggt
     5941 caaggagccc gtactgcgcc gggatgtgat cgagaacggt cagtgcgaag acgacgagcc
     6001 cggcagcaaa cacgaagaaa ccgatcatcg gcgggtaaaa aagctccacg tagctgtcac
     6061 ggatgtattg tgcgccgggg aagagcatcc agaggacggc cccggcaagc aggaggccgg
     6121 caccccaccg tccctcgtcc ttgaaccgga accgcggtgc ggccctgtct ctccagcggt
     6181 acttcccgag ctcggctccg aagaaggcga tcgcaagcca ggggaagacc gggaaccagc
     6241 cctccatgag ccaggcccgg gcgatctcga caggtgccgg gaacgggtcg ccggagatgg
     6301 agaagggggt gagcgtgacg tcgggtctcg tgatgaccgc ccccgaatag cccaacccca
     6361 cctggagaag aggtgttaga cagaggatcg cgacgatgac ggcccagcgt ccccgctccg
     6421 ggaggccgag atagaggtag gtgagcggaa gagcaaggcc gatgaggtag agcacatcga
     6481 cgtcaaggaa cgggacgtag tcaaagatca gcacctggag aagcgccccg gcgaggatga
     6541 agagcccacc cctcgtggcg atatacgaga agggcctgcc cttgccggtc cgtgagaggg
     6601 cgaccatcat accggcaaga ccgatgaaga tcggggctgc aagggaggag aggaaacggt
     6661 accagcccgg tgcagggggc aggaggagcg ccggcgtgag gtttgccatg atcatgaacg
     6721 tgatggcaag gccccggagt atgtcgacgg ggatatcacg aaaagggcca gctttggaga
     6781 ctggtttcgg aggcgggtac tccatatgtt gcgtgtgatc tttaagggat aaattttgct
     6841 gaatactctt gccctgagga ggggcgctcc ccgggagata acgttatccg gaccaggaat
     6901 catcctgtgc tcggggcagg ggacgtatcg cgcttcagcc cgtgcggccg gccgtgcatc
     6961 cagaacatcc cagggaagga atatatacac cggagacatg ccagccctga cgacattcgg
     7021 gagtctgtca tgactgcacc ggaagatatc gatgcaatgc gcgatgcccg ggacgtcgca
     7081 gggttgatcc gggccctcga tgacgccgac gagtttgtca gggcgcaggc ggccctctcg
     7141 ctcgggacga tcgccgaccc ggaggcacga gagccgctcg accggatgcg aagagaggat
     7201 ccgagcgtct cggcccgcga ggccgccgcg atcgcgtaca aatggttggt cggccgcctc
     7261 gaggaggtcg aggcggtcag gggacccaaa cccccgccgc cctggtagat gcggctcaca
     7321 ggacaaagaa gttgagccag aggatggcgg tgccgatccc gaccgtgacg aagagcacga
     7381 gcatccccac cttcaaaaac tctacaaagg agatagtgag accgttccgc gccgcgatcc
     7441 cgagcacgac gacgtttgcc gatgctccga tggccgtccc gttcccgccg aggcaggcgc
     7501 cgagcgagag cgcccaccag agcgggtagg tgttcatcga tgcgcccata tcgtggatga
     7561 gcgggatcag ggtggcggtg aggggtatgt tgtccacgac ggctgaggca attgccgaga
     7621 accaggcgat gatcagcatg gcctcgccct ccgagtggac gctctcgacc acgaacccgg
     7681 caacggccgc aatcgtcccg gtctcgacga gagcgccgac gacgatgaag agcccgccga
     7741 agaagaagag ggccggccac tcgatcttct caaagatctc ttccgggggc tgccggctcc
     7801 agaagagaag gatcgacgcg ccgatcaggg cgacgagggc gggttcgagg ccgaggaggt
     7861 cgtggatgaa gaacatcccg atgacgagcg cgatgacggc gaccgatttc ttgaagagcg
     7921 atatgtcgcg gatcgcctcc tgctcgttca actctgcaaa cgtcttctcg atcactccct
     7981 tctctcccgg cgagacatgg agctcttttc ggtatatgag ggcgagcatc cccatgacga
     8041 cgacgatgtt gacgaccatg atgggggcga ggttgaccag gaactcgttg aacgtcagac
     8101 cggcggccga acctatcatg atgttcgggg ggtcgccgat gagcgtcgcc gctccgccga
     8161 cgtttgaagc gaatatctcg gcgatgagga agggcagggg agtgatccgc atgacgcctg
     8221 cgatgtagag gagcatcgga gtgatgagga ggaccgtcgt gacattgtcg aggaacgcgc
     8281 tgatgacggc ggtcacgagc gaaaagagga gcagtacccg catcgggctt ccttttgcaa
     8341 gctttgccgt ctttatggcg atatactcga ataagccgct cccgcgggcg gtgttgacga
     8401 tgatcatcat ccccatcagg aggaagatgg tgccgagatc gacgtagacc ggtatcttct
     8461 cccacgggac gatgtggagg aagacgatga ccgaggcgcc gagcatggcg gcgaccgccc
     8521 ggtgaatccg ctcatcgatg atgagcgcgt aggtgaagag gaagatcgcg atggcgatca
     8581 gttccgggcc gatcacgccc tgtcaccccg gtgatggttg ggggcggagg cggcggagcg
     8641 ggtaatgcat gccgtgagag attgacgaat ggtcataccg ttcctcaaaa agaatgaatt
     8701 gccgggagtt aggtttgcgc gctcctcccc ataatcatgc cggtcgcgcc gtctatcggg
     8761 ctctcccgca caccggggag agcccccccg gttcaggccg gcgttcgacc tttctcctcg
     8821 ctccgcaacc ggaggatctt atagatctcg cctgcaagga ggatcggcag accgaggagg
     8881 aagagcagtc cccagtcggc aagggtcagt ggcgcggtgc cgaggaattc ctgcaggatc
     8941 gggacgtaga ccaccagcgc ctggagaccg aggctcccga ggatggcaag tatcagggtg
     9001 gggtttgaga agaacccgat ccggtggagg gggaaccgga acgaccggaa gttcagcacg
     9061 ttatacatct caaatatgat caggccggtg aacgccatcg tccgggcccg gtcgatatcg
     9121 acctcgtaga agccggagaa ggcgtagagt gtcaggagac cgagccatgc cccgatgacg
     9181 aggatgacca gaagagcgga tcgggtgagg atggactcgt ccgggttccg tggccgcctc
     9241 tgcatgacgt ccttctcggc gggctcgaga ccgagcgcaa gagcggtcgg gccgtcggtg
     9301 atcaggttga tccagaggat ctggacgggg atgaggatca gcgggagacc catgaggagc
     9361 ccgccgacga tcgcgacgat ctctccgacg ttcgaggaga ggaggtagcg ggtgaaccgg
     9421 gcgatgttgt cgtattctcg cctcccctcc tcgacgccgg cgacgatgct tgcgaagttg
     9481 tcgtccacga gcaccatgtc gcttgactcc ttcgcaacgt ccgtcccctt gatgcccatg
     9541 gcgataccga tgctcgcctt cttcaacgcc ggcgcgtcgt tgacgccgtc gccggtcatg
     9601 gcgatgactt cgcggtcgcg ggagagtata tcgatcaccc ggagtttgtg ctccgccgtg
     9661 acccgggaga ggaccttcgt cgtccggaga cggcgcgcga gctcgtcgtc ggtcatcgcc
     9721 tcgaggtcgg ctcccgtcag cgccccctca ctcgagaggc ccaggtcctg cgcgacggcg
     9781 gaggcggtca gggggttgtc ccccgttatc atgatgacgt cgataccggc gctccggcag
     9841 agccggatag cctccgccgc ttcagggcgt gggggatcta cgatcccggc aaacccgagg
     9901 aagatcaggt ctttctcgac cgcctccgcc gtccggggaa cgtcgcccgg gagcgggcgg
     9961 tatgccgccc cgagtacccg gagcccccgc gatgcatacc cgcagagttc ctgttggagg
    10021 gcgtcgcgga ggtcgtcggt gagcggcacg accgcaccgt cccggaacag acgggacgag
    10081 aggtcgagga ggacttccgg cgctcccttc acgtaagcga cgtcgccgtc gccctcgcgg
    10141 tagatgatcg tcatccgctt ccgggttgag ttgaacgaga actcgcggac cgcttcggga
    10201 gcgtcgtccc gggagaggcc ggccttcgcc gccgcgacaa cgagcgcccc ttcggtcggc
    10261 gtgccgaaga tccgccactc ccccgcctcg tcgccggcaa atgtcgcgtg gttgcagagg
    10321 agcgctgcgc gcaggaactg ccggagatcg gggtccgcga gagggtcgat ggcgctccca
    10381 tcacggagga actcaccctc cggggcgtaa ccggtcccgg tcaccgccac ctcgctctcc
    10441 ggtgtcctca ccctgacgac cgtcatctcg ttccgggtga gcgtcccggt cttgtccgtg
    10501 catatgaccg agaccgcgcc gagggtctcc gatgcaggga gacggcggat aaggcagttt
    10561 cgccgcatca tgttcttgat gccgatggca agcgtcagcg tcacgaccgc cggaaggccc
    10621 tccgggatca ccgccaccgc gagcgagacg ccgatgagga acatctccag gaactcccgc
    10681 tgctgcagga agccgacgcc gatcgcgagc acagcaatac cgacggcgat gatcccgatg
    10741 tcgcggccga ggacgtccat ctgccgcgcc agaggcgtcg cttcggctct gacccgctgg
    10801 gagaggccgg cgatcaggcc gaactccgtc ctcataccgg tggcgaccgc gacaccccgc
    10861 ccccacccgt tgacgaccgt cgtacccgca aagaccatgt tgctccgttc ggcaagcggg
    10921 gtttccggag tgagcggtcc cgtcgcctta tcgacgggag aggactcgcc ggtcagcggg
    10981 gcctcgtcca cctgaagcga cgttgcctcg acgagcgaga ggtcggcagg cacccggtcc
    11041 cccatatcga gggcgacgag atcccccggc acgatcccgg atgcatcgat ctcctgctgc
    11101 ttcccgtcgc ggatgacaac ggccttctgg acgagcatct gccgcagcgc ctcgatcgcc
    11161 tccccggcct gccactcctg ggagtagccg aggagagcgt tcaggacgac gatgatgagg
    11221 atggcggcgg cgtcttgtgg ttcgccgacg aagaacgaga ccaccgcggc aacgatcagg
    11281 atgacgatca ggatgcttgc aaactggcgc aggaagacct ggaggcgcgt ctctcgctcc
    11341 tcctcgcgga ggacgtttgc cccgtagcgc tggaggcgcg cctccgcctc ctctcgagag
    11401 aggccatgcg ggccggtgcc gagcgtctgt tcgacctcat ctgaggagag ggtataccat
    11461 tcggcaggaa gggcctcctc gcggtccccg gtcggcacga tcatactttt tcggttttgc
    11521 ggcgggccat aaatagatag agatgtcgtc tcggtgactg cagcaacccg gctctcgccg
    11581 gtaacattat atccccccgg ggggttcgat agcaaccggg ccgagatccg gtccccggga
    11641 ggaatgacga tgagcaggat agcagtcctg ataacggata tgttcgagga tgtcgagtac
    11701 acagagcccg ccgcggcgtt tcgtgaggcc ggccacgatc tcgtccatgt cggcctctcc
    11761 gcaggagaga ccgttcacgg caagaagggg gaggcagagg tcaccatcga ccgggccgtc
    11821 tccgacgtat cggtggatag cttcgatgca ctcttcatac cgggcggcta ctcgcccgac
    11881 aaactccggg cccacgatgc gcccgtgaag ttcgtgcagg agtttgtcca gagcggcaaa
    11941 ccggttctct tgatctgcca cgcgcctcag ctcctgatca cggcgcaggc cctctccggc
    12001 cggaggatca ccggctggag atccgtcgcc caggatatca agaacgccgg tgcggagtac
    12061 gtcgaccagg aggtggtggt cgacggcaac ctggtgtcga gccggcagcc ggacgatata
    12121 cccgcgttcg tcagggcgtc gcttgagaag cttgcagagg ccggcgggaa ggtagaggcc
    12181 gcggctgtgc ggcagcggtg agaccgcgag ccgggatggc aatcccggca catcctttta
    12241 tttgcacagc cagatctcat gcatgggaat gaagcccgca ggatcgacat ggagcagacc
    12301 cgctgtcatc gtcctgactg cggtgctcat cgtcctcctg ggggccatcg ctcgcgtgct
    12361 caccggtatc gatgccgcgt acaccgatct cctctacctc gtgctcatcc cgaccgcgta
    12421 ctggtaccgg cggcatgcgg tataccccgg gatactggtc gcggtgctcc acatcgtcgc
    12481 tacatacatc gtcaccgggc atgtgggtgc ggagacgctc gtgcgtgcgg ggatgctcat
    12541 cgtcattccc tacgtcctcg ggtacttctt cgaggtcgtg ggccggcgga ccgagaagaa
    12601 acattttcat gccggcgaat ccgggccggt cgcatgcgac cggagcacga agaggttgat
    12661 agcccggttg tcgagccggg acccggatac ccgctacgag gcggccgggt gcctcggcaa
    12721 cgcgggcgat cccgctgcgg tcctgcccct cgccgccctt ctccgcgatc cggagagcgg
    12781 cgtacgctgg aaggcggcgg aagcgctcgg aaggctgggt tcgccggccg tggaaccgct
    12841 ttcagggagc ctgcagagcg aggatgtgga tgtccgatgg atggccgcgg tcgctctcgg
    12901 cgatatcggc gacccggcgg ctgttccggc gctcgcaaag gcgctcgacg atgatgacgc
    12961 ctacgtcagg agcagggcgg ccctcgcgct tgcggctatc ggggaacccg cgcgggagat
    13021 gctcatcacc ggtctttcca ccgggaacgc ccgggtccgg gggagcgcgg cgctggcgct
    13081 cgggaggctt gggggcgaga gcgccaccgg ggctctcatc gacgccctcc gcgacccgga
    13141 cgaggaggtc cggcaccggg cagcggtcgc tctcggggat atcggggagc ctgccgtccc
    13201 cgatctcctc tcggccaccc gggccgacga ggcggacgtc cggcgggggg tgaccgccgc
    13261 cttcggccag atcgggaaac ccgccgtcgc cccgcttatt gcagcgctcg atgacgatga
    13321 ctggcgcatc cgcaggggag cggcggcggc tctcggcgat ataggcgatc ccggatctgt
    13381 cgacgctctg atcagggcgc tcgacgatgc gcgggaggag gtgcgagaac aggcccggaa
    13441 agcgctcggc agcatcagga agacataaca tcgacgaaga tgcacctgaa cggtatgact
    13501 gacgagaccc ggattgatga actggttgcc gacctccggc ggggccctct tgcggtgcgg
    13561 cgggcggcga ccgccgaact cggggcgagc ggggccgtgg cggtcgaacc gctgattgcc
    13621 gcgatgcttg acggcgacaa cgacctccgg tggtatgccg cccgggcact ggtgcagatc
    13681 ggtgaggctg cgatcgatcc cctcctcgcg gcgatgcggg ccggggagaa ccgtgatttc
    13741 cggcgatacg ccatggcggc cctggccggg ataggagagg ccgccgtcga acctctcatc
    13801 gatatcatcg agtgcgccga cccggccctc caaccgtttg ccgcgatggc cctcatgaag
    13861 atcggcgagc cggcggtcga accgctcctc cgcctgatgc agagcccgga cccgaagacc
    13921 caggaacgcg ctgcgctcct cctctgcagg atgggagaga cgggtgccga acctctcacc
    13981 ggagcgctgg aagctgaccg gtccggaaaa aggtgatatt ggcctctttc tgctacaggg
    14041 tgcgggcaat tgattttttc tcggttgagg cggcgggtga tcgcctgcag ggtctcaacc
    14101 ccatccccgt actctccggg atcgaagatc tccctgccgg cgggactttg cgcccggcaa
    14161 gaagtctcta aatgcgtgaa gggctcaata gttcctgtat ggaggtctca cctgctatcg
    14221 agcgatactt cgagaacctc gcggccgggc ttgattccgc catggcactc gccgctgccg
    14281 cgcgggcccg gggactcgat ccgagaacgg agatcgagat cccggtagca agcgaccttg
    14341 ccgatcgcgt cgaggcgctc ctcggctatc cgggcatcgc ggcccggatc cgcgaacttg
    14401 aggcggagat gtcccgtgag gaggcggccc tccggatagg cgacgatttt gcagcgagga
    14461 tgtttggcga gaccacgacg gaggagatcc tcgaccacgc catccgtggc gcgatggcgc
    14521 tcctgaccga aggggtggtg gccgccccga cggagggtat cgcgaaggtc agcctcggga
    14581 agaacgacga cgggaccgat tatctcaaga tctactacgc ggggcctata cggagcgccg
    14641 gcgggacggc gcaggcgctc tcggtactgg tgggcgacta cgtccgccag gcgctcggca
    14701 tcaaccgcta cgtcccccgc cctgaggagg tggagcgcta catcgaggag atccggcagt
    14761 acaacaacat catgagcctg cagtacctcc cgagcgagaa ggagctccgg acgatcatca
    14821 cgaactgccc cgtctgcatc gacggtgagc cgaccgagca gcaggaggtg agcggctacc
    14881 ggaacctgga gcgggtggag acgaacaccg tccggggcgg gatggcgctc gtcgtcgcgg
    14941 agggactggc cctgaaggcc ccgaagatcg tcaagaacgt caagaagatg aagatggcgg
    15001 gctgggattg gcttgaagag atgatcggcg gcggtggtgc ggcaaagagc gatgacgacg
    15061 acaagggcgc cgccgtcaaa ccgaaggaca agtacctccg ggaccttatc ggcggccggc
    15121 cggtcttttc gtacccgatg cgcaaggggg ggttccgcct gcgcctcggc cggtcgcgga
    15181 acaccgggtt tgcggccgcc ggcttaaacc ccgcgacgat gcacatcctc ggcgatttcc
    15241 ttgcggtcgg gacccagatg aagatcgagc ggccgggcaa agcggcgggg atcgtgccgg
    15301 tggactctat ccaggggccg acggtcaaac tccggagcgg tgaggtccgc cgggtggacg
    15361 atgcggcgga agcgcggcgg atagccggcc aggtcgatga gatcctggat gacggcgaga
    15421 tcctgatcag tttcggggag ttcatggaga acaaccaccc gctgatgccc ccgtgctact
    15481 gcgaggagtg gtggcggctc gagggtggtc cgcggcaccc ggcaagcgaa cttgaggcta
    15541 tagagttcgc gctcgacggc atccctctcc accccgacta cacctacctc tgggacgacg
    15601 tcgccccggc ggatatcgcc ctcctcgccg accggatcag cgccggcggc cggatcgagg
    15661 gtggagtgct gacgctgccc gatacaccgg aggcgaaggc gatcctcgag gagctcctgg
    15721 tcccccaccg cctctccggg gaccggatcg cgattcccgg gtacctggtc ctcctcgcct
    15781 gcctcggtct gacgctgcac ctcgataaaa ggccggcgtg ggagaacgct ccccttgaga
    15841 actcgcttga ccttgttatg cacctctcgg gcttttcgat ccgctcccgg gcgggcaccc
    15901 gggtcggcgg caggatgggg cggccgggga agtcaaagca gagggagatg aaaccgccgc
    15961 cccactcgct cttccccatc ggggacgagg gcggttctcg ccggtcgttc caggcggcct
    16021 gcgcctcacg gccccggtcg aacacggacg gcggcgtgat cgaggtggag gtgggcgagc
    16081 ggcgctgccc ctcctgcggc acgttcactt acaagaacct ctgcgattgc ggggcgcaca
    16141 cgcggccggt cctccggtgc ccgaagtgca accaggagat cggtaaggat atgtgccccc
    16201 ggtgcagcgt tgcgacggtc tgcctccaga aggtttcgat caacgtcagg atcgagtatg
    16261 cggcggcgct cgcgaacctc ggcgtgcgcg agtcggcgat cccgctcctg aagggtgtca
    16321 aaggcctgat ctcccgcgag cggccggtgg agccgatcga gaaggggatc ctccgggcgt
    16381 tgcagaacct ttatgtcttc aaggacggga ccgtccgcta cgatatgatc gatctccccc
    16441 tgacccattt ccggccggac gagatcggcg tcccggtcga gcggctccgc gaactcggct
    16501 acacccacga catctacggc cgggaactcg tcgagaccga ccaggtgctg gagctccgcc
    16561 accaggatat cctggtctcg gaagactgcg gcgagtggct tgtccgcgtg gcgaagttca
    16621 tcgacgacct cctggtgcgg gtttacgggc ttgagccctt ctaccgggcg gagcaacctc
    16681 tcgaccttat cggccacctc ctgatggggc ttgcgccgca cacgagcgcc ggtgttcttg
    16741 tccggcttat cgggttttcg aaggccggtg tcggctacgg ccacccgttc ttccacgcag
    16801 ccaaacggcg gaactgtttt gcgggcgaca cggagatcgc cgtttccgat ggccggcagt
    16861 ggattacggt gccgatccgg cagttcgtgg tggagaactt cgatctctca aagccggggc
    16921 tcgaccggac gggaacgttc tactccgacc cccggcagcc gttctatgca cgaagcatcg
    16981 acccgcaggg gctggtcggc gtgaaaaaga tcacgtcggt ctcgatccac cgcgccccgg
    17041 cgcacctcat ccggttcgtg acggggcggg ggaaggtcct ctcggtcacg cccgaccacg
    17101 cgatgctggt ctgggatacc gattacctcc ggaagatccg ggcgctcgaa gtgaagatcg
    17161 gcgaccgggt cccggtggag gagggggggc tcgtcatcag cgacgtggtc gtgagccgcg
    17221 agaccgtcca ggcgctcgac gaccgggtct actgcctgac ggttgcggag aaccacaccc
    17281 tggttgcaaa cgggatcttc tgcgggcagt gcgacgggga cgaggactgc gtgatgctcc
    17341 tcctcgacgg cctgatcaac ttctcccgcg cgtatctgcc ggagacccgg ggcgggacga
    17401 tggatgcccc gctcgtcctg acgacccgga tagacccggc ggaggtcgac aaggagagcc
    17461 tgaacgtcga tgtctgcgac cactaccccc tcgaggtcta caacggctgt cttgcctacg
    17521 cccacccaaa agacctcgac aaatacgtcg accgggtgga gcggcgcctt gggacgccgg
    17581 cccaggtcga ggggttcttc ttcacccacc cgacatccga catatcggcg gggccgcttg
    17641 agtccaccta cacgaagctc ggtacgatgc ttgagaaact cgatgcggag cttgacctcg
    17701 caaagaagat acgggcggtg gatgccgacg atgtggccga gcgggtcttg aacacccact
    17761 tcatccgcga cctccaggga aacctcaacg ccttctccaa gcaaaaggtg cggtgcatga
    17821 agtgcaacgc gaagtaccgg cggatgccgc ttgccgggaa gtgcacccgc tgcgggggaa
    17881 acgtcatccc gaccgtccac gagggttcgg tgaagaagta cctggatatg tcgcgcaaca
    17941 tatgcgagac ctacgcgata accgagtaca cgaagcagcg ggtggaggtg ctcttcatgc
    18001 agatagagtc gaccttcggc gagcccccgg agcggcagct cgggcttgcg gacttcatgt
    18061 gagggaggat ggaggcgaga gaagtcctct cggccatcag ggccgggatt cctatccggg
    18121 gcgaccggcg gcggcccgag atcgagccgt tgccggggat ccgtgcatac cggctgacga
    18181 cacctgcaac gcgagcagat cggacgatcc tcttcttcca tgcgggtggg tttacgtccg
    18241 gctcgacggc cgattctttc gatctctgtg cccggctttg cgatgcggcg agatcggagg
    18301 tcgtctcggt cgactaccgg ctcgcgccgg cacacccctt cccggcggcg gtggaggatt
    18361 gtctctcggc ctaccgctac ctgctccgcg agggctattc gccggagcgg gtggtgccgg
    18421 ccgggctctc ggccgggggg acgcttgcgc tctcgatgct tctctctgcc cggggcgcgg
    18481 ggctcccgat gccggcggcc ggggtcctgc tctcgccggc ggtcgacatg ctcttcctcg
    18541 gcgaatcggt cctcctcaac cgggagaccg actggctcgc gccggagcag tttgcggcga
    18601 tgcgggagga ttacctcgcc ggcggcgatc cgtgcgatcc gctggcctca ccgctctacg
    18661 cggacctttc ggggttgccg ccgctcctcg tgcaggcggg gggcggagag gtgctgatcg
    18721 atggaattga ggcgttcgtg agcaccgccc gcctccaggg ggtgccggtc gccttcgact
    18781 gcacggaagg gatgtttcac tgctggcagg cgtttgccgg ggtactcccg gagggcgcga
    18841 tggcggtcga gcggatcggg gcgttcgtcc gccggcacgt gccggatgag ggatgagggg
    18901 ccggcggggc atcaagctca ttactctgga ggttcaagaa gtacaggcgc gagggtagcc
    18961 aagcggtcaa cggcggtcga ctcaaga
//
LOCUS       DAOSII010000024        18788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_3173_length_18788_cov_47.555757, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000024 DAOSII010000000
VERSION     DAOSII010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 18788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18788
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3173_length_18788_cov_47.555757"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..961
                     /locus_tag="PLV90_03200"
     CDS             116..961
                     /locus_tag="PLV90_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOF96153.1"
                     /translation="METMMMQKKLIWTLLALLCAGMLVSAASAAPTTELHVAKIASDG
                     VTVINETTVDYRWMEKNLPVLGDGVTHYYHQGPVFEGDKWDPLETTNFKGRGAVKGTN
                     IRDLAELVGGAAPGDEIMVRAADGYHVEFAYENVYNPDPRQGPIGICWYNGAEAGAGE
                     RQGCGYVPDYYTGMRLVFFADNSTNSEGLNVYGNQDMYDTLPESAQHFYDLYPSTGGL
                     TVKWVDEVRVYAGGFSGERGALAGSLTTPVAKATTEAAKAPLSLMVTLAAALGAVALH
                     ARREA"
     gene            958..1524
                     /locus_tag="PLV90_03205"
     CDS             958..1524
                     /locus_tag="PLV90_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOF96154.1"
                     /translation="MIPVRRISLPLILVLIGALALGAGCTDTGSVDDYPAWNLTLAGD
                     REQVLTHPEIRALPAAEGYGYSVSTVGIKTGPNRYRGVLLADLIDMAGGAGADDLIYV
                     SAEDGYLWVFDMDQVNGEGFFTFDENLREVTSPPLRVILAYEQDKEPLAYENGGPLRL
                     VVITESPDVITEGSPWVKWVDRVEVHRK"
     gene            1521..2555
                     /gene="wtpA"
                     /locus_tag="PLV90_03210"
     CDS             1521..2555
                     /gene="wtpA"
                     /locus_tag="PLV90_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tungstate ABC transporter substrate-binding
                     protein WtpA"
                     /protein_id="HOF96155.1"
                     /translation="MNRSPVLAVLILAVLAASAGCADAGPERTILMVIPAGSLLVPLE
                     DIEAAFESQHPDIDVRVEGHGSIQAIRQVTDLGREVDVVLVADASLIPDMMYLPMGAG
                     EGNYTDWYIPFAENEVVIAYTGRSAGAGEITAENWYRILSRPDVRVGFSNPMLDACGY
                     RAIMVTALAEEYYGEPGLFEAIIGGSFKPPIAAVWTDGATTIALPELMRPAAEKVAIR
                     DGSIYLLSLLDAGGIDYAFEYRSVAEEHGLRWIDLPPAINLGSPEHADDYRKVRVNLG
                     FQRFRSIGSERIGQPIVYAMTIPANAPHPAEARIFVDFILDAFREGRAGWPDPVRQDP
                     GTATVYHATD"
     gene            2690..3979
                     /locus_tag="PLV90_03215"
     CDS             2690..3979
                     /locus_tag="PLV90_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOF96156.1"
                     /translation="MFWNRAIETIRPEELADLQLKRLKWALRQAQEVGLYQRKFREAG
                     ISPDDIRTLDDVEKLPFTYKKELQAGYPFGLFAVPMREIVRIHTTSGTTGKPTVVGYT
                     RQDLDNWADLIARNMTMIGLGVDDVFQNAVNYGLFTGGLGFHYGAEKIGMTVIPSATG
                     NTRRQIEMIEDFGVTAIHCTPSYALHLAEVAESMGKTLDTLKTGIFGAEPWSENTRVE
                     LERRLGVKAYDSYGLSEMYGPGVAFECPEHNGLHIWHDCYLTEVIDPVTGERLAPGER
                     GEMVITPLVKEALPLVRYRTGDITRLLEGECACGRGQKIERITGRSDDMLVIRGINIF
                     PSQIEHVLRSLPEVGDQFMVYVDRVRHLDEMTIEVEMTRAGFSGELQDLARVQKKIVG
                     ELHSTLGLRTAVKLVEPGSLPRFEGKAKRVIDRRGAV"
     gene            3979..5280
                     /locus_tag="PLV90_03220"
     CDS             3979..5280
                     /locus_tag="PLV90_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOF96157.1"
                     /translation="MAPWNRQIEEMPPDELRQLQYRLAKSLIRRLYETSEFYRRRMKE
                     HDVRPDDIGSLADIGKLPFMYKGDLRDNYPDGLFCAQKDELVRYHVSSGTTGKPTVVG
                     YTRQDIENWSESLARAFSSCGLGRGDVLQVSYGYGLFTGGLGVHYGAERVGATVLPTS
                     VGNTERQIELMQDLHTTAIACTPSYLLHMGEVAEKMGVSIKNDTDLRIGILGAEPWSE
                     QMRGRLEEWLGIRAYDIYGTSELSGPMFTECSEQQGIHIWGDIALVEVVDPETGEALP
                     PGERGEMVVTMLQKEAFPLVRYRIGDITVLDEEPCACGRTHPRIRRIQGRVDDMLIVR
                     GINVFPSQVEHVLLEIPEVGRHFQIVIDRKGALDTMLVRVEVSEEAFSDKITELMVIK
                     KAVEHRLKTTLNVAVGVELVEPGTLPRFEGKSKKVVDRRSL"
     gene            5280..5711
                     /locus_tag="PLV90_03225"
     CDS             5280..5711
                     /locus_tag="PLV90_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOF96158.1"
                     /translation="MEKSYIIKQISIFSENRPGRLAAVAGALRDAGINIFAFSIAEAN
                     GFGVVRALVNRPEDAHRTLTGLGFRVSFTDVIGVKMRDEPGGLSEVASVLGDAGINIE
                     YAYAYSGKDAAVLILRVDKVEDAVRQILDHGGELLKASLFR"
     gene            complement(5769..7058)
                     /locus_tag="PLV90_03230"
     CDS             complement(5769..7058)
                     /locus_tag="PLV90_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hexulose-6-phosphate
                     synthase/ribonuclease regulator"
                     /protein_id="HOF96159.1"
                     /translation="MATPVLQVALDLLELDRAVEIADEAVRGGADWIEAGTPLIKSEG
                     MRAVRTLRERFPGREIVADMKIADTGAVEVEMAAKSGAGIVCILADADDTVVAEAVRS
                     ARKYGVRIMADLINIDDPVARSREVAALGVDYINAHVGIDQQMIGRSSLDLLRRLAGE
                     VDIPIAVAGGLDATTASEAVAAGASIVIVGGNIIKAADVTAAAQRVRAAIDRPDIRPR
                     EEESPDVAIRRLLEAVSAPNVTDAMHRKGAMAGVVSLCGRVKAVGRAVTVRTVAGDWA
                     KPVEAIDVAGPGDVLVISNDGGTHIAPWGELATHSAKNRGVSGVVIDGAVRDVDDIRE
                     MAFPVFARATVPNAGEPKGLGEINAEIACCGQEVRPGDWIVADESGVVVVPRERAYEV
                     ARRAMEVKKTEERIREEIRRGKTLSEVSELLKWEKRH"
     gene            7166..8125
                     /locus_tag="PLV90_03235"
     CDS             7166..8125
                     /locus_tag="PLV90_03235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HOF96160.1"
                     /translation="MVDIGYKLASEEHGPLDLVCSARQAEDAGFSFAMISDHYHPWTT
                     RQGESPFVWGVIGGISQVTADLELVTGVTCPTMRIHPGIIAQAAATAAAMMPGRFVLG
                     LGSGENLNEHIFGDRWPPAPVRIEMLEEAVEVIRALWTGSMQTYYGSFYTLENAQVFS
                     LPEILPPIAIASEGPISASLAARVGDGFINGGTNAEESLVVFKKSGGDDKPAYVEISV
                     CWAESEEVGERTAYEQWPIAANTGELNRLLPTPAHYEQLAKMATLEDVVEGMPCGPDP
                     EPYIKKIEDRVRMGFDHICIHQIGEQQKEFMDFAAAEILPHFR"
     gene            8232..8786
                     /locus_tag="PLV90_03240"
     CDS             8232..8786
                     /locus_tag="PLV90_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2115 family protein"
                     /protein_id="HOF96161.1"
                     /translation="MSRGEDGVDIRRVCARLRSATTRAELASILYREVSGYSLFDLQA
                     LRGRVERDLQSVPAGYRQRLYPLMMEMLFATHHTLIATMRRPDPDLPDEPLAGDFQEF
                     CGMVEKTCLTRESDQRLGLLYYLLAAFNIFVLALPAHPVGTPFPGGLTVEVRNGEYLC
                     PVREKADDVENALCPYCPAKQSDV"
     gene            complement(8890..9420)
                     /locus_tag="PLV90_03245"
     CDS             complement(8890..9420)
                     /locus_tag="PLV90_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOF96162.1"
                     /translation="MSPIGGTVNLGVTVIRSRHSVRKYKESPIEDKAIKDALDCARLA
                     PTARNEQPWLFGTIRDRDMLKAIADLAENARFIADAPICFAVFGKRDAKYYLEDCCAA
                     TTQLILALQAWGVGTCWVAGEKKDYADDVRQLLNVPDEYALVSLVPAGYPEEIQIKKK
                     KLPDEVTFFERYEEEK"
     gene            complement(9483..10679)
                     /locus_tag="PLV90_03250"
     CDS             complement(9483..10679)
                     /locus_tag="PLV90_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HOF96163.1"
                     /translation="MAEIHLVGTAHVSQKSIEDVRSAIEGFEPDIVGVELDRGRYVAL
                     TEEIAEPSIADILKGGNFGRLLVQWVLTYIQQRIGAETGVKPGAEMLVAIEEAEKHQK
                     PVAFIDRDIRITLGRFWGRMGIWEKIKLIGALIYSLVSVEGQDLDVDELTNQDVVSAA
                     MEEFRKFSPHGAQALIDERDAYLAHQILMLGNRYDRVLAVVGAGHIQGVQRYLNAPET
                     LPPLPSLTADVKGLPWGKIIGAAVTIIFLLLIAAIAFSGVGLEVLIAALVYWILINGV
                     LSAAFTLLAGGHPFSAATAFAVSWLTSLNPLLAAGWFAAIVEAKIRKPTTGELRQIIE
                     AETFSEMRRIPLFRVVLVAALANVGSSLGTVAYFVFIFPVLGIDPTVVITEGFSNMIR
                     MIAGFF"
     gene            complement(10672..11214)
                     /locus_tag="PLV90_03255"
     CDS             complement(10672..11214)
                     /locus_tag="PLV90_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOF96164.1"
                     /translation="MLGIDDVADRVGSVGFRCRECGACCRRVAEDSNLVIVSPAEVRA
                     IMAATGMTWNEVAAPYPEFIDAGNGGEYTLAWCIRRTNEACIFLRDGRCSVYAHRPWI
                     CRTYPFMLVDDDLRVSECPGLGTPLSPRETRTLGADLCRRQAAEAAEEAGVRAVYREA
                     TIPPGGRAVIDSEGVKEVHG"
     gene            complement(11289..12305)
                     /locus_tag="PLV90_03260"
     CDS             complement(11289..12305)
                     /locus_tag="PLV90_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00303 family protein"
                     /protein_id="HOF96165.1"
                     /translation="MSRPFLSKDPGLRPRRPVMALVLANTMLSTVPGISGAGPTPEKT
                     LLTPVLDAELVTTGAITSVPARPNTPTGCPTPATITRSMVELTGLSPVFINAGLVHTP
                     TVPCLDVYGSAGGDPRKGDAVPQAALLFERGETVGRLLAQYSDLLVLGECVPGGTTTA
                     LCVLRALGYDASVSSAFVENPLALKDAVCRDTLARIRKAGESEPLGILRAAGDPMMPV
                     AAGIASAYTGDILLAGGTQMLAVAATLKELGRRVPHLATTVYIRDDPSAGFSRSVADI
                     GTTAYYVDPDFAGLGHAGLARYCTGEVKEGTGAGGALMLAYLMGHEPEEITQKVVDFV
                     RKYV"
     gene            12425..12859
                     /locus_tag="PLV90_03265"
     CDS             12425..12859
                     /locus_tag="PLV90_03265"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOF96166.1"
                     /translation="MFEIEHRLEGKGIMRRDIVAAAMDLYVSHGIGADEAAKKLDEKI
                     GKAFEDPNVSSLLLSAILLEDELYWRRKNSEIADDPVFLLSDEIIGMAIAEVIGGTYA
                     RFEFTRYDQKKPGILSKLGPFLDDAVAGLIAGCTSRLYSESM"
     gene            12856..13566
                     /gene="cobS"
                     /locus_tag="PLV90_03270"
     CDS             12856..13566
                     /gene="cobS"
                     /locus_tag="PLV90_03270"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HOF96167.1"
                     /translation="MKSVLALLQFCTALPLGKPVEFEHFARRSYIYPLAGYVIGGIAA
                     GVVAWIGSPAVGAAVALAVVLILSGCNHFDGLLDFGDGLMAHGSREKRIAAMTDRTTG
                     AGAVAAGIIVTLVAFAGLQSTTLLPAAILTAEVSAKVAMSYLTTLGVPFREGIHSYLH
                     GFARPWFVVPATLLALPLFLLPVSPMKIALALVAAAGIAAALLALSRHLFGGVNGDVV
                     GAANEITRAGVILLLALL"
     gene            13604..14479
                     /locus_tag="PLV90_03275"
     CDS             13604..14479
                     /locus_tag="PLV90_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOF96168.1"
                     /translation="MMKDYTLHTRGGVITERNADYATVRLRIPAGVLTADQLRQIARI
                     SVKYGDGSVHLTMRQTAEIPHVNPENLAKVAKALEKNGTPIGAEQNEVVNIMACPGTE
                     RCKYANCETIDLARKIDARVFGKELPIRLRIAISGCTYMCNSPLLNDIGIIGRIRPLR
                     IPGLCTGCGTCVEYCKQCAISLKNGISVLDEEKCVQCGVCVHSCPYHLLKSEYDHYQI
                     TVGGWRGTTPRVGRELVTVETEEEVVEVVDRIVYWVYRTAWSGRPLADQMDEIGFEKF
                     AEGIRKEFGEKAAEA"
     gene            14556..14792
                     /locus_tag="PLV90_03280"
     CDS             14556..14792
                     /locus_tag="PLV90_03280"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOF96169.1"
                     /translation="MSKVEIRPVDAQGRIILPRSWRNRFKTGYVVIVAEDDRLEIRPA
                     DADLSRFVDSVEVEAEHFDDYHDLRKELRKNAVH"
     gene            14779..15195
                     /locus_tag="PLV90_03285"
     CDS             14779..15195
                     /locus_tag="PLV90_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOF96170.1"
                     /translation="MRFIDANVFIYAILKPKAALPDTVLRKKAAAKDIFLRVNAGEPV
                     TTTTVHLSEVANVLEDAAGVAFATDLLSAILLKPAITVESVSADDYRESIHLARNHAI
                     GINDALAVVVMEREGISEIYTFDRHFRETRVKVVQE"
     gene            complement(15293..16108)
                     /locus_tag="PLV90_03290"
     CDS             complement(15293..16108)
                     /locus_tag="PLV90_03290"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOF96171.1"
                     /translation="MTDGEGQRDRRHAYRAVGYIPADTFLHRMDPRTKLLTVLAISIA
                     AFATISPLRTAILFAFVVCLAVAAGLVRPFSRGLLLLLPLLVFIVVIDIFFPEISSGH
                     VYLSAQVGPFSSVITSGGLLYAAAKGIRILVIGGISFLFVMTTSYTDFVASLRISRLP
                     PVIAFSLGYALKSMTALADDLRNIMDAQRSRALEFDRGTLIKNRRKLMALGVPMTVSI
                     LNRARYVTEAMQSRGFASTPRPTCYRPPRPGRHDLLLLVALGIVCLATLLIPV"
     gene            complement(16105..17538)
                     /locus_tag="PLV90_03295"
     CDS             complement(16105..17538)
                     /locus_tag="PLV90_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF96172.1"
                     /translation="MTADPAIEFRSVSFRHPASRANRPREVLAGLDCVIGEGERVLLT
                     GPSGSGKTTFLRCCNGLIPHARPGTFKGDVFVRGRNTRDCTIGDLATTVGMVFQDPDH
                     QIFSNDVISEIAFGPEQLGWPPSRIDRAVNEILEQFRLDRLCDRQTTELSWGERQRLA
                     IASVVVLRPAILILDEPFSGLDAANAAGLLDLLERLNRESGMTIVLSEHRTEMVARWA
                     SRRISLLDGRLIEPPVPDAPLRGRPPRPGAKAARPGVVTLENVAFRHPGKVSPAMDGV
                     SLTLYAGEATVLAGPNGSGKSTLLRLLNGLLRPESGQIVVNGRDVRESTVAEIARTVG
                     LVCQHADYQLFGETIAEELAFAPANLGTPRDVIEQRVERAREGLGIEHLGLTASPLAL
                     SVGEKQRVSIASVLTMDTQVLALDEPTLGLDPAAKQRLAGLIDALCAHGKAVVIATHD
                     AGFSAAVADRIVRLERGRIVDDRRVQA"
     gene            complement(17528..18085)
                     /locus_tag="PLV90_03300"
     CDS             complement(17528..18085)
                     /locus_tag="PLV90_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOF96173.1"
                     /translation="MMKRPSYFSLYEIALLSLFGALVFVLRTAIIVPIHVPGSSGIFW
                     VLPLIVGTAIVRKPGAGTYAGLVSGILASFFGIEPLHVFDVFKYTAMGITIDLVSMAF
                     GYRLENPVVGFVAGAAGNVVKMVVNYAVHLLLGVQGVFILLGIGLSSATHIVFGGIGG
                     IIAALIVGRLYRAGIVHQDDVPDDG"
     gene            complement(18082..>18788)
                     /locus_tag="PLV90_03305"
     CDS             complement(18082..>18788)
                     /locus_tag="PLV90_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96174.1"
                     /translation="AYARESAFADSLPHTAAIDRYDLRYGPQSWRPYATPIAIGPGDL
                     PSGDWYIAFVELGVVPVGGNPALARTGSVRVDYRFTDLAGTASLGVYGLHAGDGRAWT
                     NRQSGYGTSGYYVSGTAAPGDLPRWTTPLEAGNRYVVEVAGSPRPVDEDVARDTCTIW
                     FERISSGLDGIHITTDRAAPKGQVTETADLDGSFYITYTGGNRVGALFLMVAVDRPQP
                     EGFGLALESTFVEISR"
BASE COUNT         3479 a         5999 c         6177 g         3133 t
ORIGIN      
        1 gcggggtgac gatgcatccg gcaggggccg tcatcgatac cgtccgcctc aatacggccg
       61 gcgggtggtg gagcgtgctc gcatccgccc ccggcgtatg ggaattatca ggattatgga
      121 gacgatgatg atgcagaaga aactgatatg gacgctgctc gcgctcctct gcgcgggcat
      181 gctggtaagc gccgcatcgg cggcgccgac gaccgaactg cacgttgcaa agatagcatc
      241 cgacggggtc accgtcataa acgagacgac ggtcgattac cgctggatgg agaagaacct
      301 cccggtcctc ggcgacggcg tgactcacta ctaccaccag ggaccggtct ttgaaggaga
      361 caagtgggat cccctggaga cgacgaactt caagggcaga ggagccgtga aaggaacgaa
      421 cattcgtgac ctcgccgaac tggtcggcgg cgccgcaccc ggcgacgaga tcatggtccg
      481 ggcggcggac ggctaccacg tcgagtttgc ctacgagaac gtctacaacc ccgacccgcg
      541 ccagggcccc atagggatct gctggtacaa cggcgccgaa gcgggagcag gcgagcggca
      601 ggggtgcggc tacgtccctg actactacac cggtatgcgc cttgtcttct ttgcggacaa
      661 ctcgaccaac agcgagggcc tcaacgtcta cgggaaccag gacatgtatg atactctccc
      721 cgaatcagcg cagcacttct acgacctcta tccctccacc ggcggcctca cggtgaagtg
      781 ggtggacgag gtccgggtct atgccggcgg attctccggc gaacgggggg cccttgcggg
      841 atccctcacc accccggtgg caaaagcgac gaccgaggcg gcaaaagccc cgctctccct
      901 catggtgacg ctcgccgcgg cactcggggc ggtggcgctc catgcccgga gggaggcatg
      961 atcccggtgc gccggatctc tctccccctc atcctggtgc tgatcggtgc cctggccctc
     1021 ggggccgggt gtaccgatac cggttccgtc gacgactatc ctgcctggaa cctgacgctt
     1081 gccggagacc gggaacaggt cctcacccac ccggagatca gggccctccc ggccgccgaa
     1141 ggctacggct actcggtctc gacggtgggg atcaagaccg gcccgaaccg ctaccgcggc
     1201 gtgcttcttg ccgatctcat cgatatggcg ggcggcgccg gggcggacga cctgatctac
     1261 gtctctgcag aagacgggta tctctgggtc tttgatatgg accaggtgaa cggagagggg
     1321 ttcttcacct tcgatgagaa cctccgggag gtcacgtcgc cgccgctccg cgtgatcctg
     1381 gcctacgagc aggataaaga accgctggcc tacgagaacg gcggaccgct ccgcctcgtc
     1441 gtcataaccg agagccccga cgtcatcacc gaagggagtc cctgggtgaa atgggtcgac
     1501 cgggtcgagg tccacaggaa atgaaccgct ctccggtcct cgccgtgctc atccttgccg
     1561 tgctcgcggc atccgcaggc tgcgccgatg ccggcccgga gcgcaccatc ctcatggtga
     1621 taccggcagg cagcctcctt gtcccgctcg aggatataga ggccgccttt gagagccagc
     1681 accccgatat cgacgtcagg gtcgaagggc acgggagcat ccaggccatc cggcaggtga
     1741 cggacctcgg gagagaggtg gacgttgtcc tggtcgccga cgcctcgctc atcccggata
     1801 tgatgtacct gccgatgggt gccggggagg gaaactacac cgattggtac attccctttg
     1861 cggagaacga ggtggtgatc gcgtataccg gccggagcgc cggggcaggc gagatcacgg
     1921 cggagaactg gtaccggatc ctctcccgcc ccgacgtgcg ggtagggttc tcaaacccca
     1981 tgcttgatgc ctgcggctac cgggcaatca tggtgacggc gcttgcagag gagtattacg
     2041 gcgagcccgg tctcttcgag gccatcatag gcggttcatt caagccgccc atcgcggcgg
     2101 tctggacgga cggcgccacg acgatcgccc tgccggaact gatgaggccg gcggccgaga
     2161 aggtcgccat ccgtgacggg agcatctatc tcctctcgct cctcgatgcc ggcgggatcg
     2221 actacgcctt cgagtacagg agcgttgcgg aggagcacgg actccggtgg atcgatcttc
     2281 cccccgcaat caacctcggt tcgccggagc acgccgacga ctaccggaag gtgcgggtga
     2341 acctcgggtt ccagcggttc cggtccatcg ggagcgagcg gatagggcag ccgatcgtct
     2401 acgcgatgac catcccggca aacgcaccgc accccgccga agcgcggatc ttcgtggact
     2461 tcatcctcga tgcattccgc gaggggaggg ccggatggcc cgaccccgtg cggcaggatc
     2521 cggggactgc cacagtgtac cacgctacgg attgacaaac cttttccgca ccggacaccc
     2581 atgtaaaggt agcgtatgcc cggcggcgcg ggctctctcc gccgccagga tcgcaaccgt
     2641 atgacggatg ccaggcagca tgcggccggc acgataccga tgggatatta tgttctggaa
     2701 cagggcaata gaaacaatcc ggcctgaaga actcgcggat ctccagctga agcggctcaa
     2761 atgggcgctt cgccaggcgc aggaagtagg gctataccaa agaaagttca gggaagcggg
     2821 catatcgccc gacgatatca ggacgcttga cgatgtggag aagctcccgt tcacctacaa
     2881 aaaggagctc caggcaggat acccgttcgg cctctttgcg gtccccatga gagagatcgt
     2941 ccggattcac acgacatccg gcacgacggg gaaaccgacc gtcgtcggct acacccggca
     3001 ggatctcgac aactgggcgg acctgatagc gcggaatatg acgatgatcg gtcttggagt
     3061 agacgatgtc tttcagaacg cggtcaacta cggcctcttc accggcggtc tcgggttcca
     3121 ctacggcgcc gagaagatcg gcatgacggt gatcccgagc gcaacgggaa acacccgccg
     3181 ccagatcgag atgatcgagg acttcggggt gaccgccatc cactgcacgc caagttacgc
     3241 ccttcacctc gccgaagtgg ccgagtcgat gggaaagacg ctcgatacac tcaagaccgg
     3301 catattcggg gcggaaccct ggtcggagaa cacgcgggtg gagctcgaac ggcgcctcgg
     3361 ggtgaaggcc tacgacagtt acgggctttc ggagatgtac ggcccggggg tggcgtttga
     3421 gtgcccggag cacaacggtc tccatatctg gcacgactgt taccttacgg aggtcatcga
     3481 tcccgtgacc ggcgaacggc tcgcccccgg cgagcgggga gagatggtca tcacgccgct
     3541 cgtcaaagag gccctgccgc tcgtccgcta ccgcaccggc gacatcaccc ggctgctcga
     3601 gggcgagtgc gcctgcgggc gcggacagaa gatcgagcgg atcacgggcc ggagcgacga
     3661 catgctggtt atccggggga tcaacatctt cccgtcacag atcgagcacg tgctccggtc
     3721 tcttccggag gtcggggacc agttcatggt ctacgtcgac cgggtacggc acctcgacga
     3781 gatgacgatc gaggtggaga tgacccgggc cgggttctcc ggcgagctcc aggacctcgc
     3841 ccgcgtccag aagaagattg tcggcgaact ccacagcacc ctcggcctcc ggaccgcggt
     3901 gaaactggtg gagcccgggt cgctgccccg gttcgagggg aaggcaaaga gggtcatcga
     3961 ccgccggggg gccgtctgat ggcgccctgg aaccggcaga tcgaggagat gccgcccgac
     4021 gaactgcggc agctccagta caggctcgca aagtcgctca tccggcggct ctacgagacg
     4081 agcgagttct accggcgacg gatgaaggag cacgatgttc ggcccgacga tatcgggtcg
     4141 cttgcggata tcgggaaact cccgttcatg tataagggag acctccgcga taactaccct
     4201 gacggcctct tctgcgcaca aaaggatgag ctggtccgct accacgtctc atcggggacg
     4261 accgggaagc cgaccgtcgt cggctacacc cggcaggaca tcgagaactg gagcgagtcg
     4321 ctggcgcgcg cgttctcctc ctgcggcctc gggcgcgggg acgtgctcca ggtgagttac
     4381 ggctacggcc tcttcaccgg cggcctcggc gtccactacg gcgccgagag ggtcggcgca
     4441 accgtcctcc cgacaagcgt cgggaacacc gagcggcaga tcgagctgat gcaggacctc
     4501 cacaccaccg ccatcgcctg caccccctcc tacctcctcc acatgggcga ggtggcggag
     4561 aagatgggcg tctcgatcaa gaacgacacc gatctccgga tcggcatact cggcgcagaa
     4621 ccctggtcgg agcagatgcg gggccggctt gaggagtggc tcggtatccg cgcctacgac
     4681 atctacggca cgagcgaact ctccgggccc atgttcaccg agtgctccga gcagcagggc
     4741 attcatatat ggggcgacat cgccctcgtc gaggtcgtcg atcccgagac cggcgaggcg
     4801 ctcccgccgg gtgaacgggg cgagatggtc gtcaccatgc tccagaagga ggcgttcccc
     4861 ctggtccgct accggatcgg agacatcacg gtcctggacg aggagccctg cgcctgcggg
     4921 cggactcatc cgcggatccg ccggatccag gggcgcgtgg acgacatgct gatcgtcagg
     4981 ggcatcaacg tcttcccctc acaggtggag cacgtgctgc tcgagatacc cgaggtcggc
     5041 aggcacttcc agatcgtcat cgaccggaag ggcgcgctcg ataccatgct cgtgcgggtg
     5101 gaggtgagcg aggaggcgtt ctccgacaag atcaccgaac tcatggtgat caagaaggcg
     5161 gtggagcacc gcctcaagac cacgctgaac gtcgccgtgg gcgtcgaact ggtcgagccg
     5221 ggaaccctcc cgcgctttga gggcaagtca aagaaggttg tcgacaggag gtcattgtaa
     5281 tggagaagtc gtatatcatc aaacagatct caatcttttc ggagaaccgc ccggggcgcc
     5341 ttgcggcggt cgccggtgcg ctgcgggatg cgggaatcaa catatttgcg ttcagcatcg
     5401 ccgaggcaaa cgggttcggt gtcgtcaggg cgctcgtcaa ccggccggag gatgctcacc
     5461 ggacgctgac ggggctagga tttcgggtat cgttcaccga cgtcatcggc gtgaagatgc
     5521 gcgacgaacc cggcggcctc tcggaggtcg catcggtcct cggggatgcg gggatcaaca
     5581 tcgagtacgc ttacgcctac tcggggaagg atgcggcggt ccttatcctg cgcgtggata
     5641 aggtcgagga tgccgtccgg cagatcctcg atcacggcgg agaactcctc aaagcgtccc
     5701 tcttccggtg accacaagag cataacccta ccgcggggac ggggggccgc accgacgccg
     5761 gcccccgctc aatgccgttt ctcccacttc aggagttccg agacctcaga gagcgtcttt
     5821 ccgcgccgga tctcctcgcg aatcctctcc tcggtcttct tgacctccat cgcccggcga
     5881 gcgacctcgt aggcccgctc ccgggggacc accacgacac cgctctcgtc ggcgacgatc
     5941 cagtctcccg gccgcacctc ctgaccgcag caggcgatct cggcgttgat ctccccgaga
     6001 cccttcggtt cgccggcgtt cgggacggtc gcccgggcaa agaccgggaa tgccatctcc
     6061 cggatgtcgt ccacatcccg gaccgccccg tctatcacga cgccgctaac cccccggttc
     6121 ttcgcggagt gggtggcaag ttcaccccag ggcgcgatat gggttccccc gtcgttactg
     6181 atgacgagga cgtcgccggg tccggcgacg tcgatcgcct cgacgggttt tgcccagtcc
     6241 ccggcgacgg tccggaccgt caccgcacgg ccgaccgctt tcaccctgcc gcagagggag
     6301 acgacgccgg ccatggctcc cttccggtgc atggcgtcgg tgacgttcgg cgcagagacg
     6361 gcttcaagga gccggcggat ggcgacatcg gggctctctt cctcccgcgg gcgtatatcg
     6421 ggccggtcga tggcggcccg gacccgctgc gccgccgcgg tcacgtcggc ggccttgatg
     6481 atgtttccgc cgacgatgac gatcgaggcg ccggcggcaa ccgcctcgga tgcggtcgtc
     6541 gcgtcgagcc cccccgcgac ggcgatgggt atatcgacct cgccggcaag gcggcggagg
     6601 aggtcaagcg acgaccgccc tatcatctgc tggtcgatgc cgacgtgggc gttgatgtaa
     6661 tcgacgccga gcgccgcaac ctcccgcgac cgggcgacgg ggtcgtcgat gttgatgagg
     6721 tcggccatga tccggacgcc gtacttccgg gccgaccgga ccgcctcggc gacgacggtg
     6781 tcgtccgcat cggcaaggat gcagacgatg ccggcgccgg actttgccgc catctccacc
     6841 tcgacggccc cggtatcggc gatcttcatg tccgcgacga tctcgcggcc ggggaagcgc
     6901 tcccggagcg tccgcacggc ccgcatgccc tcgctcttga tcaggggggt cccggcctct
     6961 atccagtccg caccgccacg aaccgcttca tccgcgatct ctaccgcacg atcgagctcg
     7021 aggagatcga gcgccacctg gagaaccggt gttgccatac tatactgtag ggcggagaac
     7081 gtatttatct ggtgccgggc cgggggaaac ttatatgccg tgcggtgaag gaggtagccc
     7141 gcccaccgca tggatgtgat gatgaatggt cgatatagga tataaactcg cgagcgagga
     7201 gcacgggcct ctcgatctgg tctgtagcgc ccgccaggcg gaggacgccg gttttagttt
     7261 tgccatgata tcggatcact accacccctg gaccacccgg cagggggaga gcccgtttgt
     7321 ctggggggtc atcggcggca tctcccaggt gacggcggac ctggaactgg tgacgggcgt
     7381 cacctgcccg acgatgcgga tacaccccgg catcatcgcc caggccgccg caaccgccgc
     7441 cgcgatgatg cccgggcggt tcgtgctggg cctgggatcg ggcgagaacc tcaacgagca
     7501 tatattcggc gaccgctggc ccccggcgcc cgtcaggata gagatgcttg aggaggcggt
     7561 ggaggtgatc cgggcactct ggacggggag catgcagacc tactacggct cgttctacac
     7621 gcttgagaac gcgcaggtct tctcgctccc cgagatcctt cccccgatcg ccatcgcatc
     7681 cgagggcccc atcagcgctt ccctggccgc gcgggtcgga gacgggttca tcaacggggg
     7741 caccaacgct gaggagagcc tcgttgtctt caaaaaatcc ggtggcgatg ataaacccgc
     7801 atacgtggag atatcggtct gctgggcgga gagcgaggag gtgggagaga ggaccgccta
     7861 cgaacaatgg cccatcgccg caaacacggg agaactcaac cggctcctcc ccacgccggc
     7921 ccattacgag caactcgcga agatggcgac gcttgaagat gtggtggagg gcatgccctg
     7981 cggcccggac ccggagccct acatcaaaaa gatcgaggac cgggtccgga tggggttcga
     8041 ccatatctgt atccaccaga tcggagagca gcagaaggag ttcatggatt tcgcggcggc
     8101 cgagatcctg ccgcacttcc ggtagagcgc cggcgtcggg cgcacggggg cagtcgaccc
     8161 cgaaccggcg ctcgcggcac ccgcccggaa gagctgattc atgcattctg ccgaccaaga
     8221 ttaatcgcgt gatgagccgg ggagaagacg gggtggatat ccgccgggtc tgcgctcgcc
     8281 tccggtcggc aacgacccgg gcagaactcg cgtcgatcct ctaccgcgag gtctcgggct
     8341 actcgctctt cgacctccag gcgttgcggg ggagggtgga gcgggatctc cagtccgttc
     8401 ccgccgggta ccggcagcgc ctctaccctc tgatgatgga gatgctcttt gcaacgcacc
     8461 ataccctcat agccaccatg cgccggcccg acccggacct ccccgacgag ccgctcgccg
     8521 gagatttcca ggaattctgc gggatggtcg agaagacgtg tctcacccgg gagagtgacc
     8581 agcgcctggg cctcctctat tacctccttg ccgccttcaa catcttcgtc ctcgccctcc
     8641 ccgctcaccc cgtgggcacg ccgtttcccg gcggactcac ggtcgaggtc aggaacggcg
     8701 agtacctctg cccggtccgg gagaaggccg acgacgtcga gaacgccctc tgcccttatt
     8761 gcccggcaaa gcagagcgac gtgtgatgcc ggcaccccgg aggctccggg gcctgccggc
     8821 accgggaaag acgtcagtga ataccgggca tccatggcat gataaaaagt gagttcgtgc
     8881 agtatcgacc tacttctctt cctcgtagcg ctcgaagaac gtgacctcgt cggggagttt
     8941 cttcttcttg atctggatct cttcggggta gcccgcgggc acgagggaga cgagcgcata
     9001 ctcatcgggg acgttgagga gttgccggac atcgtcggcg taatccttct tctctcccgc
     9061 aacccagcag gtcccgaccc cccaggcctg gagcgcgagg atcagctggg tcgtcgccgc
     9121 acagcaatct tcaaggtagt acttcgcatc tcgtttcccg aagactgcaa aacagatggg
     9181 cgcatcggcg atgaaccggg cgttctcagc gagatccgcg atcgccttca gcatgtcgcg
     9241 gtcccggatc gtcccgaaga gccagggctg ctcgtttctt gccgtcgggg caaggcgtgc
     9301 gcagtcaagg gcatccttga tggccttatc ctcgatcggg ctctctttgt acttccgtac
     9361 gctgtgccgg ctccgtatga cggtgacgcc gaggttgacc gttccaccaa tcggactcat
     9421 gcaacgatgg tgggcactcc aggcctaaat accttgggga aacggataac cgggggagga
     9481 gctcagaaga agcccgcgat catccggatc atgttcgaga agccctcggt gatgaccacg
     9541 gtcgggtcga taccgaggac ggggaagatg aagacgaagt aggcgacggt cccaagcgaa
     9601 ctcccgacgt ttgcaagagc ggcgaccagc accacccgga agagcggtat ccgccgcatc
     9661 tcggagaaag tctccgcctc gatgatctgc cggagttccc ccgtcgtggg tttacggatc
     9721 ttcgcctcga cgatggctgc aaaccatccc gccgcaagca gggggttgag cgacgtcaac
     9781 caggagacgg caaacgccgt ggcggcagag aaaggatgtc cgccggcgag cagggtgaac
     9841 gccgcgctca ggacgccgtt gatcaggatc cagtagacga gggcggcgat caggacctcg
     9901 agccccacgc ccgagaaggc tatagcggcg atcaggagga ggaatatgat ggtgacggcc
     9961 gccccgatta tctttcccca ggggaggccc ttcacatcgg cggtcaggga cgggagcggc
    10021 ggcagggtct ccggcgcgtt gaggtagcgc tgcaccccct ggatgtgccc ggcgccgacg
    10081 accgcgagca cccgatcgta ccggttcccg agcatcaaga tctggtgcgc aaggtaggcg
    10141 tcccgttcgt cgatcagggc ctgggcgccg tgcggagaga acttccggaa ctcttccatc
    10201 gcagcgctca ccacgtcctg gttcgtgagt tcgtccacgt cgaggtcctg gccctcgacg
    10261 ctcacgagcg agtagatgag ggcgccgatg agtttgatct tctcccagat ccccattctg
    10321 ccccagaacc gcccgagcgt gatccggata tcccggtcga tgaatgcgac gggtttctgg
    10381 tgcttctcgg cctcctcgat ggcgaccagc atctcggcgc cgggtttcac gcccgtctcg
    10441 gcgccgatcc gctgctggat gtaggtgagc acccactgga cgaggagccg gccgaagttc
    10501 ccgcccttca gtatatccgc gatcgagggc tcggcgatct cttcggtgag cgccacgtac
    10561 cggccccggt cgagctcgac gccgacgata tcgggctcga acccctcgat ggcggaacgg
    10621 acgtcctcga tgctcttctg cgagacgtgg gccgtcccga caagatggat ctcagccatg
    10681 gacctccttc acgccctcgc tgtcgatcac ggcgcggccc ccgggcggga tcgtcgcctc
    10741 ccggtagacg gcccggacgc cggcctcctc cgccgcctcg gcggcctgcc gccggcagag
    10801 gtcggcgccg agtgtgcggg tttcgcgggg agagaggggc gtgccgagcc cggggcattc
    10861 cgagacccgc aggtcgtcgt ccacgagcat gaacgggtag gtgcggcaga tccaggggcg
    10921 gtgggcgtat acggagcacc gcccgtcgcg gagaaatatg caggcttcat tcgtgcgcct
    10981 gatgcaccag gcgagggtgt actccccgcc gttcccggcg tcgatgaact cggggtaggg
    11041 cgcggcgacc tcattccagg tcataccggt cgccgccatg atcgcccgca cctcggccgg
    11101 gctcacgatg acgaggtttg agtcctccgc gacccggcgg cagcaggcgc cgcactcccg
    11161 gcaccggaac ccgacggacc cgacccggtc cgcaacatca tctattccga gcatatccgg
    11221 ttctcaaaga aaggtcgtcc gtggaaaaca ttgcttatag gtggatgtaa aaaggtatag
    11281 tccttccctc agacatactt ccggacgaaa tcaacgactt tttgggtgat ctcctcaggt
    11341 tcgtggccca tgaggtaggc gagcatgagc gccccgccgg cccccgtccc ttccttgacc
    11401 tcgccggtgc agtaacgggc aaggccggca tgcccgaggc cggcgaagtc cgggtcgacg
    11461 tagtacgcgg tggtgccgat atcggcgacc gaccgggaga aaccggccga gggatcgtct
    11521 ctgatgtaga ccgtggtcgc aaggtgcggc acccgtctcc cgagttcttt caaggtagcc
    11581 gccacggcga gcatctgggt cccgccggcg agcaggatat ccccggtata ggcgcttgct
    11641 atccctgccg ccaccggcat catggggtcg cccgcggccc ggaggatacc gagcggctcg
    11701 ctctcgcccg ctttgcggat ccgggcgagg gtatccctgc agacggcatc tttgagggca
    11761 agagggttct ctacgaacgc gctgctgacc gaggcgtcgt agccgagcgc ccgcagcacg
    11821 cagagggcgg tcgtcgtccc gccggggacg cactcgccga gcaccaacag gtcgctatac
    11881 tgcgcaagga gccggccgac cgtctctccg cgctcgaaga ggagcgccgc ctgcggcacc
    11941 gcgtccccct tccgggggtc gccgcccgcg ctcccgtaga cgtccaggca ggggacggtg
    12001 ggggtatgca cgagcccggc gttgatgaag acgggggaga gaccggtcag ctccaccatg
    12061 gaccgggtga tcgtcgccgg cgtcgggcac cccgtggggg tgttcggccg ggccggcacg
    12121 ctcgttatcg cgccggtcgt gaccagttcc gcgtccagga ccggcgtgag gagagtcttt
    12181 tccggtgtcg gcccggcacc cgatatgccg gggacggtgg agagcatggt gttcgcgagg
    12241 accagcgcca tcaccgggcg gcgcggcctg agaccggggt cttttgagag aaaaggacgg
    12301 gacatccggt ataaggtggg tgccgcgggt agaagaggtt gcggtctttc gacgcgatat
    12361 ccagtggaac ccggtacgca cggaatccaa acgcgcaaat accgccaggc tcaaccacta
    12421 tgtcatgttc gagatcgagc acaggctgga agggaagggt atcatgagga gggatatcgt
    12481 cgccgccgcg atggatctct acgtctccca cgggatcggg gcggatgagg ccgcaaagaa
    12541 gctggacgag aagatcggga aggccttcga ggaccccaac gtatcgtcgc tccttctttc
    12601 cgccatcctg ctcgaggatg aactctactg gaggaggaag aactccgaga tcgccgacga
    12661 cccggtcttc ctcctctcgg acgagatcat cgggatggcc atcgcagagg tgatcggggg
    12721 aacctacgcg cggttcgagt tcacccgcta cgaccagaag aagccgggga tcctctctaa
    12781 actcgggccg ttcctcgacg acgccgtcgc cgggctgatc gcgggctgca cgtcgcgcct
    12841 ctatagtgaa tcgatgtgaa gtcggtcctt gccctcctgc agttctgcac ggcgctccct
    12901 ctcgggaaac cggtcgagtt cgagcacttc gcccgccgct cctacatcta cccgctcgcg
    12961 gggtacgtga tcggcgggat agcggccggc gtcgtcgcct ggatcggatc tccggcggtg
    13021 ggtgcggccg tcgctctcgc cgtggtgctc atcctctcgg ggtgcaacca cttcgacggc
    13081 ctgctcgact tcggcgacgg gctcatggcc cacgggagcc gggagaagcg gatagcggcc
    13141 atgaccgacc ggaccacggg ggccggcgcc gtcgcggcgg gtatcatcgt cacgctcgtc
    13201 gcgtttgccg ggctccagag cacgacgctg ctccctgcgg ccatcctcac ggccgaggtc
    13261 tccgcgaagg tcgctatgtc ctacctcacc acgctcggtg tgccgttccg ggaggggatc
    13321 cactcctacc ttcacgggtt tgcacggccc tggttcgtcg tcccggcaac cctccttgcg
    13381 ctccctctct tcctgctgcc ggtctctccg atgaagatcg cgctcgcgct cgttgccgcg
    13441 gccgggattg ccgcagcgct cctcgcgctc tcccggcacc tctttggcgg ggtcaacggc
    13501 gacgtcgtcg gggccgcaaa cgagatcacc cgggcggggg tcatcctcct cctcgccctc
    13561 ctgtaaccat tctttttatc ccggcgaccg ccactactgt aggatgatga aggactacac
    13621 cctccacacc cggggagggg tcatcaccga gcgaaacgcc gattatgcaa cggtacggct
    13681 gcggattccg gcaggtgtcc tcaccgcgga ccagcttcgc cagattgcgc ggatcagcgt
    13741 gaaatacgga gatggctccg tccacctcac catgcgccag acagcggaga tcccgcacgt
    13801 gaaccccgaa aacctcgcga aggttgcaaa ggccctcgag aagaacggga cacccatcgg
    13861 ggcagagcag aacgaggtgg tgaacatcat ggcctgcccg gggacggaac ggtgcaagta
    13921 cgccaactgc gagacgatcg atcttgccag aaagatcgat gcgcgggtct ttggaaagga
    13981 gctcccgata cgcctccgca tcgccatatc gggctgcacc tacatgtgca acagtccgct
    14041 cttaaacgac atcggtatca tcggccgtat ccggccgctg cgcatacccg gtctctgcac
    14101 ggggtgcggc acctgcgtgg agtactgcaa gcagtgcgcc atatcgttga agaacggcat
    14161 atccgtcctc gacgaggaga agtgcgtcca gtgcggcgtc tgcgtccact cctgccccta
    14221 ccacctcctg aagtcggagt acgaccatta ccagatcacg gtcggcgggt ggcgggggac
    14281 cacgccccgg gtagggcgtg aactcgtcac cgtcgagacc gaggaggagg tcgtcgaggt
    14341 cgtcgaccgc atcgtctact gggtctaccg gactgcctgg agcggccggc ctcttgccga
    14401 ccagatggac gagatcgggt ttgagaagtt cgcggagggg atccgaaagg agttcgggga
    14461 gaaggcggcg gaggcgtgaa gaggggccgg ccggtgtgat ccgggcgatg gggtaaatct
    14521 taatacctgg tgatacgaag taaccctggt gatttatgtc aaaagtggag ataagaccgg
    14581 tcgatgccca ggggcggatc atcctgccgc gatcgtggcg caaccggttc aagacaggct
    14641 acgtggtcat cgtcgccgaa gacgaccggt tggagatacg accggcagat gcggatctct
    14701 cgcggtttgt ggatagcgtt gaggtcgagg cggagcattt cgacgattac cacgacctcc
    14761 ggaaggaact gagaaagaat gcggttcatt gatgcaaacg tcttcatata cgccatcctg
    14821 aaaccgaagg ccgccctgcc ggataccgtc ctgcgaaaaa aggctgcggc aaaagatatc
    14881 ttcctccggg tgaatgccgg cgaaccggtc accacgacca ccgtgcatct gagcgaggtg
    14941 gcaaacgtgc tggaagatgc tgcgggagtg gcgtttgcaa cggatctcct ctcggccata
    15001 ctcctgaaac ccgccatcac tgtcgaatcg gtctctgccg atgactaccg ggagagcata
    15061 cacctcgccc ggaaccacgc catcggcatc aacgacgccc ttgccgtggt cgtcatggag
    15121 cgagagggga tcagcgagat ctataccttc gaccggcact tcagagagac ccgggtaaag
    15181 gtagtgcagg agtagccccc ctgcagatct tttggtgaac tgctgccgtg gcccgtgcag
    15241 gccacttccg cgccagccgg gaggctgatc gagccccctg ccatggaaac ccctacacgg
    15301 ggatcagcag cgtcgcaaga cagacgatgc cgagcgcgac gagcagcagg agatcgtgcc
    15361 tcccgggccg gggagggcgg tagcaggtgg gcctcggggt cgacgcaaac ccccggctct
    15421 gcatcgcttc ggtcacgtag cgggcccggt tcaggatcga taccgtcatc gggacgccga
    15481 gcgccatcag tttcctcctg ttcttgatga gcgtcccccg gtcgaactca agcgcccggg
    15541 agcgctgggc gtccatgata ttccggagat cgtcggcaag cgccgtcatc gacttcagcg
    15601 cataccctaa cgagaaggcg atgaccgggg gcagccggct gatgcgaagg ctggcgacga
    15661 aatccgtgta tgatgtggtc atcacgaaga ggaacgatat cccgccgatg acaaggatcc
    15721 ggataccttt tgccgcggca tagagcagcc cgccggaggt tatcaccgac gaaaacgggc
    15781 cgacctgggc ggaaagatac acgtgccccg aggagatctc ggggaagaat atgtcgatca
    15841 cgacgatgaa gaccagcagc ggcaggagga ggagaagtcc ccgggagaac ggtctcacga
    15901 gccctgcagc aaccgcaagg cagacgacga aggcaaagag gattgcggtg cggagaggcg
    15961 atatcgtggc gaacgcagcg atgctgatcg caagcacggt caggagcttg gtcctcgggt
    16021 ccatccggtg aaggaacgta tccgccggta tgtagcctac cgcccggtac gcatgcctcc
    16081 ggtcgcgctg cccctccccg tcggtcatgc ctgcactctc cggtcgtcca cgatgcggcc
    16141 gcgctcgagc ctgacgatcc tgtccgcaac cgccgcggag aacccggcat cgtgcgtcgc
    16201 gatgacgacc gcttttccat gggcgcagag tgcatcgatg agcccggcca gcctctgttt
    16261 ggcggcgggg tcgagcccga gcgtcggttc gtcgagcgcg aggacctgcg tatccatcgt
    16321 caggacggat gcgatgctga cacgctgctt ctccccgacc gagagggcaa gcggcgaggc
    16381 ggtaagcccc aggtgctcga tgccgagacc ctcgcgcgca cgctcgaccc tctgctctat
    16441 cacgtcacgg ggagtgccga ggtttgccgg ggcaaaagca agctcctcgg cgatggtctc
    16501 cccgaagagc tggtaatcgg catgctggca gaccaggccg acggtgcggg cgatctcggc
    16561 gacggtgctc tcccggacgt cccttccgtt cacgacgatc tgcccggact ccggccgcag
    16621 gaggccgttc aagagtctca gcagcgtgct cttgcccgag ccgttcgggc cggcgagcac
    16681 cgtcgcctcg ccggcgtaga gggtgaggga gacgccgtcc atcgccggcg agactttccc
    16741 cgggtgccgg aacgcgacgt tctccagggt gacgaccccc gggcgggcgg ccttcgcccc
    16801 cgggcgggga ggccggcccc ggaggggagc gtccgggacg gggggctcga tcagccttcc
    16861 gtcgagaagg gatatccggc gggacgccca gcgagcgacc atctcggtcc ggtgctcgga
    16921 gaggacgatc gtcatgccgg actcccggtt cagtctctcg aggaggtcaa ggagcccggc
    16981 cgcattcgcg gcgtcaagcc cggagaacgg ttcgtcgagt atgaggattg ccgggcgcag
    17041 aacgacgacg gaggcgatcg ccagacgctg gcgttctccc caggagagct cggtcgtctg
    17101 ccggtcgcag agccggtcaa gccggaactg ctccaggatc tcgttgaccg cccggtcaat
    17161 tcgcgacggc ggccagccaa gctgttcggg gccgaacgct atctcgctta tcacgtcgtt
    17221 agagaagatc tggtggtcgg ggtcctgaaa taccatgccg acggtggtgg caaggtcgcc
    17281 gatcgtgcag tcgcgggtgt ttcgtccccg gacaaagacg tcgcccttga aggtgcccgg
    17341 gcgggcgtgc gggatgaggc cgttgcagca ccggaggaac gtcgtcttcc ccgagccgct
    17401 cggcccggtc agcagcacgc gctcgccctc cccgatgacg cagtcaagcc cggcaagcac
    17461 ctcccgcggc cggttcgccc gggaggccgg gtgccggaac gagaccgacc gaaactcgat
    17521 cgccgggtca gccgtcatcc ggcacatcgt cctgatgcac gatacccgcc cggtagagcc
    17581 gcccgacgat cagggcggcg atgatgcctc cgatgccccc gaagacgata tgcgtggccg
    17641 acgataaccc gatgccgagg agaatgaaga cgccctggac gccgaggagc aggtgtacgg
    17701 cgtagttgac caccatcttc accacgttcc ccgcggcgcc ggcgacaaag ccgacgacgg
    17761 ggttctcgag acggtagcca aacgccatag agacgaggtc gatcgtgatc cccattgcgg
    17821 tgtacttgaa tacatcgaag acgtgaagcg gctcgatgcc gaagaacgac gcgaggatgc
    17881 cggataccag tccggcatac gtgccggccc cgggttttct cacgatggcg gtgccgacga
    17941 tgagcggcag gacccagaat atcccggagc tcccgggaac gtggatcggg actatgatcg
    18001 ccgtccgcag gacgaagacg agagcgccaa agagcgagag gagggcgatc tcgtacaggc
    18061 tgaagtagga gggacgtttc atcatcggct aatctccacg aatgttgatt cgagcgcaag
    18121 cccgaaacct tcgggctgcg gccggtccac ggcgaccatc aggaagaggg ctccgaccct
    18181 gttgccgccg gtatacgtga tgtagaacga accgtcgagg tcggcggtct cggtcacctg
    18241 acctttgggg gccgcacgat cggtggtgat gtggatccca tccaggcccg agctgatccg
    18301 ctcaaaccag atggtgcagg tatcccgggc cacgtcctcg tctaccgggc gcggcgagcc
    18361 cgccacctcg acgacgtagc ggttgcccgc ttccagggga gtcgtccacc gcggcaggtc
    18421 gccgggcgcg gcggtcccgg agacgtagta gccggacgtg ccgtaacccg actgccggtt
    18481 cgtccatgcc ctgccgtccc cggcgtggag accgtagacg cccaacgatg ccgtcccggc
    18541 aagatccgtg aaccggtagt cgacccgaac cgatcctgtc cgggcaaggg ccgggtttcc
    18601 accgacgggc accacgccca gctcgacaaa ggcgatgtac cagtccccgg aggggagatc
    18661 gcccgggccg atcgcgatcg gcgtggcgta gggccgccag gactgggggc cgtaccggag
    18721 atcgtagcga tcgatggccg cggtatgcgg gagagagtcc gcaaaagcgc tctcccgggc
    18781 ataggcct
//
LOCUS       DAOSII010000025        18245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_3296_length_18245_cov_40.373117, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000025 DAOSII010000000
VERSION     DAOSII010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 18245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18245
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3296_length_18245_cov_40.373117"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PLV90_03310"
     CDS             <1..331
                     /locus_tag="PLV90_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF96175.1"
                     /translation="NRENKLEERIKALTKFHLLIIDEMGYLPFNREGAHCFFNLISRR
                     YEKSSTIFTSNKSYGEWGEIFGDHVIAAAVLDRILHHSTTLNIKGDSYRLKERRRQGI
                     YSHTLPG"
     gene            684..3950
                     /locus_tag="PLV90_03315"
     CDS             684..3950
                     /locus_tag="PLV90_03315"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96176.1"
                     /translation="MVEKQTNPEEVNSLWWLLSVWAGSYYRDLMYCFSHQVGQSYTVI
                     RGLYYSLRLQFVLIAIAVLAFDCLSANLADKQMDVSPINLEWVSIDVLPAIMNVLLIW
                     FQSISLIKGIELFGVILLLFLAFSLARRANVASKIVTIDEFKDCRQKNHVRGLEENVP
                     NVAEDLHHRLAAELTRIRSLYENVAGSMDVTYGYRPEPPRITADYSTTSKNISQSNQP
                     LSIGPINLPQEIIRDIASRVVRGTRITGILFEEDDRLHLVAHRIDNEEPLSWTVVGPK
                     KAHGDGTSSEPALNDMIHELACRIFTSIYTDDATEWKAVQAFNRGLEEYLHSKAKPQG
                     RTIGLIRAEEKFIETLSEDSGYHNAYHNLGVIYREQKKWQAAERVFILSTHSRPKEAE
                     SYYTLALNYFDEAYDHLYEGDIEKRGSLERDDGIFRDYWSLCRVIELCDACLMLQPVC
                     PKAYELKGFAYGLRGYITEKRPDVINRPDPSGDLDYPGKLPPFESGGHDSLDHAIQCR
                     RIAVEQSWKLLCQNLLRPSQKNDRANPLVTVTEDRDRAYKCLNNMAYTLHLKSLKRKS
                     GNSGSEKSHSTIDRLSLWLLSQIQMRIYRQALALNPSKANPHLGIGGLHLMANRPDLA
                     IESFKRAISIDYTLPTAWMYLALAYNESIANIKRIQKPGAKHQVGYPDSRSAMPGIYR
                     QGRDHALIQFLNYIEELKPLHLYRIAEEFENRIECKDITLLIRRSLAFLEETRKCESS
                     KAIDLSPPEIPSSGIEHLRSIYDDWQKGESLRVQGINFLSHGEPGKADEMFIESLQSL
                     KTLYPERIRRNAIYASQAHAQLQKKDYPTSIKTTHDALLLDPGCSHSHLRRGQGLYGI
                     NDLDHCINEYRLALQCDPEQPEIYFRIGQVDLLRAKRSHNPEEKRELSEEASNNFKIA
                     LELRKGESKDIAKMYYWLGKSSLIMGSYNEARAYLETAESLSIAPIKVAFHLGEAYLG
                     MGLFATSENYFEQVRRHLDKSIRDSTISRDSWLQSEVEEFLEDDVCAGALLARWHIRN
                     ATLAIHTSQSGEEAEKHLEAVREIIGEISSTTTRRDLESEYLRARRWLLKQKEHLKPT
                     "
     gene            complement(3961..5913)
                     /locus_tag="PLV90_03320"
     CDS             complement(3961..5913)
                     /locus_tag="PLV90_03320"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0043682 - P-type divalent copper
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HOF96177.1"
                     /translation="MGHGEEAGRETHHHIFDDYRRRFIISIILTVPVLLLSLPVGALP
                     IAFPGSAYLLLALATAVYLYGGWPFLSGFVHELRARMPGMMTLVAVAITVAYVYSSAV
                     VIGVVGGEGFYWELATLIDVMLLGHWVEMRSVLGASRALEELVRVMPSEAHRVRDGGT
                     DDVPVDRLVPGDRVLVRPGEKVPVDGVIVEGGTSVNEAMLTGESRPIEKRPGDTVIGG
                     AINGEGSIVVEVERTGAETYLARVIDLVRRAQESRSRAQDLADRAAFFLVAIALSVGA
                     VTFAVWFAIAGTGFAVERAATVMVIACPHALGLAVPLVVAVSTALAAHSGLLIRDRAA
                     FERAKDLEAVVFDKTGTLTTGTFGVTDVLAFGGAEADVVRAAASVEAHSEHPLARAVV
                     RSAEERGLSLLPVEGFRALPGKGVEGSVGGRRVSVVGPGYLAERGMAAGDERVEPLQR
                     QGKTVVFLIEGDRLTGALALADTIRPESGEAISRLKAMGIRCMMLTGDHHDVAAWVAG
                     ELGLDEFFAGVLPHEKAAKIREIRRRYRVAMVGDGINDAPALVEADLGIAIGAGTDVA
                     VESADIVLVRSDPGDAAAIIDLAKKTYRKMVENLLWATGYNAVAIPLAAGVLFGAGIV
                     LTPAAGAVLMSASTVIVAVNARMLRV"
     gene            complement(5915..6130)
                     /locus_tag="PLV90_03325"
     CDS             complement(5915..6130)
                     /locus_tag="PLV90_03325"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HOF96178.1"
                     /translation="MTLIRLLVFLAVPVYRDVNRPGVNGLLRFIPAFLILVIRRPGAG
                     DAAMDLFRQRCARGEIDEEEFQRERRG"
     gene            6537..8852
                     /locus_tag="PLV90_03330"
     CDS             6537..8852
                     /locus_tag="PLV90_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF96179.1"
                     /translation="MHEPIPAYGSISALSAVLDELDEGLLVVGSDNRIACINKRLRHI
                     LGIDRDAAGGTDATRFVHQTLAPRIAEESCRRDLLEFFSGRQRSLDLSCTLLTSGGKS
                     NRVCCSCKTVGSGLFQGMRVARFETVPREDSASPGWIGDLLRQDDDWYRFLVENLNEG
                     IGLIDREGIVVFANKRLADIIGCPIAETIGAPASRFTDEEGARCMEDYLQNLDTFPHE
                     IFEIDLIRRDGIRIHALMATTPVIDASGACRGFLAGVLDITPLKQMELQLEESKEKYR
                     SLVELSAEAILIYQDGIVTYINPAGVRLLGAQDPGEVIGTNVADIIHPGSRETVRSFA
                     ARNLQRGETPLVELPVVRLDGTMVFVEGRGTRAFFAGRPAVQVVMRDITHRKQAEERL
                     QASNRHLLLLNRIIGISAQAQTPDELLETALDQTLDLLGYDGGGIYLTEPGRSGVALR
                     YERGMPKRCLERAGELFRASYAEVCATGAPRYIEQDDSPVSCLLRDIGFTALACTPFT
                     VEPDVAGALLVGSRNRQSFPPEERALLEAVGREIGAGMLRGMLHQRLEAANREANLYL
                     DILTHDIKNADNVANIYADLLIDELEGEVVGHAQKLKDGIRKSIEITANVATIRKIHE
                     TRAGLEPVDLDAVIKSEIEHFPDLPICYDGLPLKVFADDLLPQVFTNLIGNTTKHGGS
                     KVNVAVAVEDLGEEVVVTVADTGPGIPDDMKETILYRFERENDKRGSQGLGLSICRML
                     TARYGGRIWIEDRVAGRPGEGAAFRFTLRKA"
     gene            complement(8872..10029)
                     /locus_tag="PLV90_03335"
     CDS             complement(8872..10029)
                     /locus_tag="PLV90_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04083 family peptide-modifying radical SAM
                     enzyme"
                     /protein_id="HOF96180.1"
                     /translation="MKSPFHVMIIPTLGCPSKCHYCWSSDERSPRMSIETVREITEWL
                     KGYQSNQITITFHGGEPLLAGAEFYRQALPILSEGLAHLTPTFALQSNLWLLTPDMAR
                     ILAEYRIPIGSSIDGPEEINDVQRGKGYFKKTMRGYDIARANGLEVRFICTFTNRSVE
                     RKEEIFNFFLQNGFPLKLHPALPSLRSENPKEWALEPEAYGELLVYLLDRYLENLGRI
                     EVMNINDICRGVFTRHGTVCTFVDCMGTTLAIGPDGSIYPCYRFVGMPEYVMGNVYDR
                     PGPEDLARSDAWRLMQEFREYVDRECKGCPHIKYCRGGCPYNAIAPTDGEIRGVDPHC
                     TAYRRIFDEITDRVNKELYDSTDMEMGDFGSRQRRRARPGIMSILQAMAER"
     assembly_gap    10254..10353
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10570..10998
                     /locus_tag="PLV90_03340"
     CDS             10570..10998
                     /locus_tag="PLV90_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOF96181.1"
                     /translation="MDFSECVKFANENPVTYIATMDGDQPRVRAFVMWFADATGFYYH
                     TGTPKAVWRQLAKNPKVELCFYAPGDVNTMMRVAGAVEFLDDAALKERLVGDRPWLLQ
                     IGISGPADPKLAVFRVAHGEAYFWTMEHNMREADAPRVRF"
     gene            complement(11007..11720)
                     /locus_tag="PLV90_03345"
     CDS             complement(11007..11720)
                     /locus_tag="PLV90_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOF96182.1"
                     /translation="MDPVIEAIRQEFAAHADPDRRKSSQRFFKEEIRCYGMKTATAVT
                     VAKKYWKKVRSREKQEIFGLCEELYRSGMMEEAFVVSQWAQMLGERFEPGDIAVFRHW
                     IDTYITNWAACDGLCNHAVGDLVVRYPEHIEELKRWAQSDNRWMRRAAAVSLIVPAKH
                     GEFLDEAIAIADLLLADTDDLVQKGYGWLLKEASREHTDEVFSYVMRNKRVMPRTALR
                     YAIELMPKDLKAEAMKKDW"
     gene            complement(11901..12293)
                     /locus_tag="PLV90_03350"
     CDS             complement(11901..12293)
                     /locus_tag="PLV90_03350"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOF96183.1"
                     /translation="MVECEQDEILNHFRDLLPSIRAILLFGSRVAGYAGPTSDIDICL
                     ILTGEADRTAVHDGMLIAPEHYDIVVYDEIPWYLRGGILERHRIIYAEDPDDLDFWLY
                     KQRRIWGDMKRRQQRASSEDLLKRIGYR"
     gene            complement(12263..12691)
                     /locus_tag="PLV90_03355"
     CDS             complement(12263..12691)
                     /locus_tag="PLV90_03355"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOF96184.1"
                     /translation="MDDLVRERILQKVEVLIDRIAFIEHHLSDAVMNDRILRKALYKE
                     FQEAVESATDICALIRRILLSSAQDDYTNLDYLVGEGIISRETGRDLKAANGLRNRLV
                     HEYDGINDHIAYGAMQRLMPSLRRFSGEVLTWLNANRTRS"
     gene            complement(12811..13677)
                     /locus_tag="PLV90_03360"
     CDS             complement(12811..13677)
                     /locus_tag="PLV90_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOF96185.1"
                     /translation="MTGIIAFSDLAWGTRERGLPGGGRVGIASFMRIVDEADPAIVIF
                     AGDAAYDRCSRSGLDETEVFVDLLRDIAAAGRHCIVVEGNNDNARGTYDRVREAAEAS
                     PFLHEVSGKAETVRGIRFLGVPTGKERRMARSAEGPVDIVVAHAPLADRVWLFDLPAA
                     CIVTGHYGMMVAGIAGKAYVALDCSPASYAAIEWEGGWRRIAYVAGACRISLSPGEGV
                     AATGCDAEERRRLTGGPGELPYRAEIEALRQAKDEVAVLGREEVAGRLLAMGIKKTHI
                     ERYLGRQGRPEP"
     gene            13917..15374
                     /locus_tag="PLV90_03365"
     CDS             13917..15374
                     /locus_tag="PLV90_03365"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HOF96186.1"
                     /translation="MTNKKRLTTAAGAPVPDNQNAVTAGPRGPMLMQDVWYQEKLAHF
                     NREVIPERRMHAKGSGAFGTFTVTHDITRFTRARLFSDIGKKTDVFMRFSTVAGERGA
                     ADAERDIRGFAVKFYTEEGNWDLVGNNTPVFFIRDPHKFPDLNRAVKRDPHTNMRSAT
                     NNWDFWTSLPESLHQVTITMSDRGIPLSYRHMHGFGSHTFSMINAENERVWVKFHLQT
                     RQGIKNLTDQEAEAIVAKDRESHQRDLFESIRKGDFPRWTMYIQVMTEEQAKAMPYNP
                     FDLTKVWYKGEFPLIEVGVLELNRNPENYFQDVEQAAFNPAAVVPGIGFSPDKMLQGR
                     LFAYGDAQRYRLGVNHHQIPVNAAKCVANPSHRDGQMRVNGNAGATIGYEPNSFGEWQ
                     EQPEYREPFLALSGAGGSWNFREDDADYYTQPGKLFRLMSPGQQQVLFENTARAMGDA
                     PELIKVRHIANCLKADPAYGKGVADALGIPLDRVK"
     gene            complement(15417..15905)
                     /locus_tag="PLV90_03370"
     CDS             complement(15417..15905)
                     /locus_tag="PLV90_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOF96187.1"
                     /translation="MDARRFSVTPVGTVHAGRGVFSIEIAEPFRPALTELSGFSHMLV
                     LWWCDGVDTVECRSETTCEKPYTRGPDTIGIFATRSPVRPNPIALSVAPVLGVDAAAG
                     IIRVAYIDADDGTPVLDIKPYLPCNERVRDAKVPAWASHWPQWYEENEGFDWAAEFTF
                     GD"
     gene            complement(15963..17252)
                     /locus_tag="PLV90_03375"
     CDS             complement(15963..17252)
                     /locus_tag="PLV90_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="condensation protein"
                     /protein_id="HOF96188.1"
                     /translation="MSPRYPAPAFDVFNVYFERIYDPTMHAVFTFDGGIDAGALRKAT
                     MRTVASDPYTRSRYAEVDGRPVWEEIAEEEWARAFVVLPAGGDVDMPPPPLDVRAGPQ
                     VRVGLYRRAGGDIVAVTCHHGFCDVSGTMALARDIFATYRGIVADPGYRPPPREPYDR
                     STDRILALSSDEEQRRALAEEEPFIDRWRFPAGRMGRGTPRIASRTLAPDRLERMKAF
                     GREHGATVNDVLIGAFFLALLDTRDDPTDRGAPRSILTSADLRRRYPGCHGEGLPVNL
                     SVAYDVTLTVEEGAGLEDIIGRVTAITAHKKAGNPGLAAILFYEGIMAGGMPAVRAFF
                     DEMAGRYTSSGNKNPVFSNLGVFDPGDYLPVPGKDGRALDLLDIQYLPCVCWPYGFLM
                     AASTFCGRLTVTTAYEEGPYSTATVERFLGCVDGYLP"
     gene            complement(17316..18071)
                     /locus_tag="PLV90_03380"
     CDS             complement(17316..18071)
                     /locus_tag="PLV90_03380"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOF96189.1"
                     /translation="MRLKDKVVLVTGASSGIGLEIARLFAGEGASVVAVARREDKLAA
                     LVDQITAAGGKAVSVAGDVTREEDIRNAIRAAVETFGKLDVVVNNAGLMDRMEPVAEL
                     DDDTWNAVVDVNLTGPMRLFRGAVPEMEKAGGGAFVTIASVGGIRGARAGAAYTASKH
                     GVVGLAMNVAYAYAKKGIRSNVIAPGGVSTDILTGKSLSREGTEVYMAGMATNPRMGE
                     PIEVARVALFLASDEASLVNGAVITADAGWTAY"
BASE COUNT         3737 a         5690 c         5318 g         3400 t
ORIGIN      
        1 aaatcgggag aataaactcg aggaacggat taaagccctt acgaagtttc atctcttgat
       61 catcgacgaa atgggctacc ttcccttcaa tcgcgaaggt gcccactgtt tcttcaacct
      121 gatctcacgg cggtacgaga agtcgtccac gatctttacg tcgaacaagt cctacgggga
      181 gtggggcgag atcttcggcg accatgtgat cgccgctgca gttcttgacc gaattctcca
      241 ccactccacg acattgaaca ttaaaggcga tagctaccgg ctgaaagaac gacgaagaca
      301 ggggatctac tcccacaccc tgccgggatg agtttcgata ccattatggg atcaggtgta
      361 cacttttatt ccgcctgaat ctgtacactt ttacgccgcc gttgacaaga gcaacctgat
      421 ggagtaacag ggggaatcac gctgaaaaac atgctgtcaa attaggatgc tctctgattt
      481 gatgggccct ggaataggga ggttattcga aattctccgc tgtctagggc ttatcccctc
      541 ctcgacccgg tccatctgga ttcccgtaaa gtatatctat gatggaggag gagggcgcct
      601 ctcggagtta ctggcggatg atcttttcag gcagatgggg tgagcatttt ttgagataac
      661 ttcattgggt gcaagaatgc ggaatggttg aaaagcaaac aaaccctgag gaagtaaatt
      721 ccctctggtg gctactttcc gtatgggcgg gttcttacta tcgagattta atgtattgct
      781 tcagtcatca ggttggacaa tcttacacgg ttatacgtgg attatactac tctcttcgcc
      841 ttcagtttgt cctcatcgca attgctgtct tagcctttga ctgcttgagc gccaacctcg
      901 cggataagca gatggatgtt agcccgataa acctggaatg ggtttcgata gatgtcctac
      961 ccgctatcat gaatgtattg ctcatatggt tccagagcat ttctctcata aaaggaattg
     1021 agttattcgg agttatttta ctgctttttc tggccttctc gctcgcccga cgtgcgaatg
     1081 ttgcctccaa aatcgtgact atcgatgagt tcaaggattg tcgtcaaaag aatcatgtac
     1141 ggggtttaga ggagaatgtg cctaatgtcg ctgaagatct tcaccatcga ttggctgccg
     1201 agctgacccg gatacgctca ttgtacgaga acgtagccgg atcaatggat gtaacatacg
     1261 gttatcggcc ggaacctcca cgtatcactg cggattactc aactacctcc aaaaatatct
     1321 cccagagcaa tcaaccgctg agcataggcc cgattaacct tcctcaagaa ataatcagag
     1381 atattgcatc acgggttgta agaggtaccc gtatcactgg aatccttttt gaagaagacg
     1441 ataggctcca cctggttgcc cataggatcg acaatgaaga accactcagc tggacggttg
     1501 taggaccaaa gaaagcccat ggtgatggca catcgtcaga gccggctctt aacgatatga
     1561 tccatgaact tgcgtgcagg atatttacat cgatatatac cgatgatgcc actgagtgga
     1621 aagcggtcca ggcctttaac cggggccttg aggaatatct ccatagtaag gcaaagccac
     1681 agggtcgaac tatcgggttg attcgagccg aagaaaagtt tatcgaaacc ctctccgaag
     1741 atagcggtta ccataacgcg tatcataacc tcggggttat ctatcgcgag cagaagaaat
     1801 ggcaagcagc agagagggta tttattttga gtacccattc acgcccaaaa gaggcagaat
     1861 catattacac gttggcgctg aattattttg acgaggcata cgaccatctc tatgaggggg
     1921 acatcgaaaa aaggggttcc ctggagcgtg atgatggaat ttttcgcgat tactggagct
     1981 tgtgccgggt aattgaactg tgtgatgcat gtttaatgct ccaaccagtt tgtcctaaag
     2041 cctacgaatt aaaagggttt gcgtacgggc tcagaggata tatcactgaa aaacgtccgg
     2101 atgttataaa ccgccctgat ccttcaggag atctggacta tcctggaaag cttcccccgt
     2161 tcgagagtgg cggccacgat agccttgatc atgcaataca gtgccgtcgt atagcagtag
     2221 agcagtcatg gaagttactc tgtcagaacc tcctgcgacc atcacaaaag aatgatagag
     2281 ccaatccact tgtaacagtg acggaagata gagatcgggc atacaaatgc ctgaacaaca
     2341 tggcatatac cctccatctg aaatccctga agcgaaaatc cggcaatagc ggatccgaga
     2401 agagtcactc aacgatagat cgcctgtcgc tctggttatt atcgcagatc cagatgcgaa
     2461 tataccggca ggcattagcg ctcaatccct caaaggcgaa cccgcatcta gggataggag
     2521 gactgcacct tatggccaac cgcccggatc tggcaataga atcatttaaa cgagccatat
     2581 cgatcgacta cacacttcca accgcctgga tgtatcttgc cctggcatac aacgagtcga
     2641 tagccaacat taagaggatc cagaaaccgg gtgcgaaaca tcaggtcgga tatccggatt
     2701 caagaagcgc tatgcccggg atctatcgac aggggcgaga tcatgcactc atacagttct
     2761 taaactatat cgaagagtta aagcctcttc atctctatcg catcgccgaa gaatttgaga
     2821 ataggataga atgcaaggac attaccttat taattcgaag atcactggca ttcttggaag
     2881 aaaccaggaa atgtgagagt tccaaggcca tcgacctgtc tcccccagag atcccatcca
     2941 gcgggattga acaccttaga tcgatctacg acgactggca gaagggagag agcctccggg
     3001 tacagggtat caactttcta agtcatggag agcccggtaa agccgatgag atgtttatcg
     3061 agtcccttca atcattaaag acgttatacc cggagaggat acggagaaat gctatctacg
     3121 cctctcaagc acatgcccag ctccagaaaa aagactaccc gacgtcgatt aaaaccacgc
     3181 atgatgcttt actgcttgat ccgggatgtt ctcattctca tctgcgcagg gggcagggac
     3241 tctacggcat taatgacctc gaccattgca tcaatgaata ccgcctcgcc ctccagtgtg
     3301 atccggagca accggaaata tacttccgta tagggcaggt agatctattg cgtgcaaagc
     3361 gctcccataa ccctgaagaa aagcgggaac tatcggagga agcatccaat aactttaaga
     3421 tcgctctgga gctccgtaag ggcgagagta aggatatcgc gaagatgtac tactggcttg
     3481 gaaagtcctc tttaatcatg ggttcgtaca acgaggcaag ggcgtatctt gaaactgcag
     3541 aatcgctcag tatcgctccg atcaaagtag catttcacct tggtgaggca tacctgggta
     3601 tgggtctgtt tgcaaccagt gagaattatt tcgaacaggt ccgccggcac ctcgataaat
     3661 ccattcgcga tagtaccatc tcccgtgaca gctggcttca gtctgaggtt gaggagtttc
     3721 tcgaggatga cgtatgtgcc ggtgccctcc tggcacgatg gcatatcagg aatgcaaccc
     3781 tcgctatcca tacatcgcag agcggagagg aggcagagaa acaccttgaa gcagtccggg
     3841 agatcatagg agagatatcc agcacaacaa cgcgccgtga tctggaatca gagtacctac
     3901 gtgcacggag atggctgctc aagcaaaaag agcacctcaa acccacgtag atgacccccc
     3961 tcacacccgg agcatccggg cattaaccgc gacaatcacc gtgctcgcgc tcatcagcac
     4021 cgccccggcc gccggggtga ggacgatgcc ggcaccgaag agcacgccgg cggcaagcgg
     4081 gatagcgacc gcgttgtagc cggttgccca gaggaggttc tcgaccatct tccggtaggt
     4141 cttcttcgcg agatcgatga tcgccgccgc gtccccagga tcgctcctga ccagaacgat
     4201 atcggcgctc tccacggcca catcggtccc cgccccgatg gcgataccga ggtccgcctc
     4261 gacgagcgcc ggggcgtcgt tgatcccgtc gcccaccatc gccacccggt aacggcgccg
     4321 aatctcccgg atcttcgcag ccttctcgtg cggcaggacc ccggcaaaga actcgtcgag
     4381 gccgagctcc cccgccaccc acgcggcgac gtcgtggtgg tcgccggtca gcatcatgca
     4441 ccggatgccc atcgccttga gccgggagat tgcctccccc gactccggcc ggatggtatc
     4501 cgcgagagcg agcgccccgg tgagccgatc accctcgatg aggaagacga cggtctttcc
     4561 ctgccgctgg agcggctcga cccgctcgtc cccggccgcc atcccccgct ccgcgaggta
     4621 gcccgggccg accaccgaga cccgccggcc gccgaccgaa ccctcgacgc ccttccccgg
     4681 cagcgcccgg aacccttcga ccggcagaag agagagcccg cgctcctccg cgctccggac
     4741 gacggcccgg gcgagcgggt gctcggagtg cgcctccacc gacgccgccg ccctgacgac
     4801 atccgcctcg gcaccgccga aggcaagtac atccgtgaca ccgaacgtcc ccgtcgtgag
     4861 ggtcccggtc ttgtcgaaga cgacagcctc gaggtccttc gcccgctcaa acgccgctcg
     4921 gtcgcggatg aggagccccg agtgggccgc aagagccgtc gagaccgcga caacgagcgg
     4981 gacggcaagg ccgagcgcgt gggggcaggc gatgaccatc acggtcgcgg cccgctccac
     5041 agcaaacccg gtccccgcga tggcgaacca gaccgcaaac gtcaccgcgc cgaccgaaag
     5101 ggcgatcgcc accaggaaga acgccgcccg gtcggcgagg tcctgggccc gcgaccggct
     5161 ctcctgcgcc ctccggacga ggtcgatcac ccgggcgagg taagtctccg cgcccgtcct
     5221 ctcgacctcg acgacgatcg aaccctcgcc gttgatagcg ccgccgatga ccgtgtctcc
     5281 cggccgtttc tcgattggcc ttgactcccc ggtgagcatc gcctcgttga cgctcgtccc
     5341 cccctccacg atcacgccgt ccaccggcac cttctcaccg ggccggacga gcacccggtc
     5401 gcccgggacg agccggtcga caggcacgtc gtccgtcccc ccgtcgcgga cccggtgcgc
     5461 ctccgagggc atgaccctga cgagctcctc aagtgcccgc gacgccccga ggaccgagcg
     5521 catctccacc cagtgcccga ggagcatgac gtcgatcagg gtggcaagtt cccagtagaa
     5581 cccctctcca ccgacgacac cgatgacgac cgccgagctg tagacatagg caacggtgat
     5641 cgcgacggcg acgagcgtca tcatgccggg catccttgcc cggagttcgt ggacgaaccc
     5701 ggagaggaac ggccagccgc cgtagaggta gacggcggtc gcgagggcga ggaggaggta
     5761 cgccgagccc gggaaggcga tggggagcgc cccgaccggc aacgagagga gaaggaccgg
     5821 gaccgtcagg atgatcgata tgataaatcg ccgtcggtag tcgtcgaaga tgtggtggtg
     5881 agtctcccgg cccgcctcct cgccgtgccc cacgtcagcc cctccgctcc cgctggaact
     5941 cctcttcgtc gatctcgccg cgcgcgcagc gctgccggaa gagatccatc gctgcatcgc
     6001 cggccccggg tctccggatg accaggatca ggaaggcggg gatgaaccgg agcaggccgt
     6061 tcacccccgg gcgatttacg tcccggtaga ccgggacggc gaggaagacg agcagccgga
     6121 tgagcgtcat accccctccg gagaacaggc acgcgggccc ccctcaacca tcggatgacc
     6181 gtcacggtgc gggtgcatga atccgtccat gcggcggaat gccccccaaa gaacgtaaat
     6241 atgccggccg tttcaggtgc ggagggcctg ccccgagcac ccctgtgcgc gcaatggcag
     6301 gaacgccggg tgcgtgcctc ctcagccagg tgatgcgtga agatccgccg gagagaataa
     6361 acaggtttat atgtacaaaa gcaacagcat acatctcgta gtcagacaga tccgcccgaa
     6421 ggagttccga actcctcgat tcatctgaac cgagcccggg ttcatctgaa tcgcacggga
     6481 acattttatc ttaacataat ataaaaacat cctgagggaa gaggtgatcc cggccaatgc
     6541 acgaacccat acctgcatac ggtagtatca gcgcgctctc cgccgtcctg gacgagctcg
     6601 atgagggtct gctcgtcgtc ggttcggata atcggatcgc ctgcatcaac aagcgtctcc
     6661 ggcacatcct gggtatcgac cgagatgcgg ccggcggaac cgacgcgacc cgattcgtcc
     6721 accagaccct ggcgcctcga atcgccgaag agtcgtgcag gagagacctc cttgagttct
     6781 tctccggccg ccagcgatct ctcgacctct cgtgcacgct cttgacgtcc ggcggcaaga
     6841 gcaaccgggt ctgctgctcg tgcaaaaccg tcgggagcgg gctgttccag ggtatgcgag
     6901 ttgcccgttt cgagaccgtg ccccgggagg attccgcctc cccgggatgg ataggcgatc
     6961 tcctgcggca ggacgacgac tggtaccggt tcctcgtcga gaacctgaat gaggggatcg
     7021 ggctgatcga cagggagggc atcgtggtct ttgcgaacaa gagactggcc gatatcatcg
     7081 gttgccccat cgctgagact atcggagcgc cggcctcccg tttcaccgat gaagaaggtg
     7141 cccggtgcat ggaggactac ctgcagaacc tggatacgtt tccgcacgag atcttcgaga
     7201 tcgatctgat tcgtagagac ggtatccgca tccatgccct gatggcgacc acaccggtta
     7261 tcgacgcctc aggcgcttgc cgcgggttcc ttgccggggt cctggatatc acccccttaa
     7321 agcagatgga gctgcagctt gaggagagca aagagaagta tcggtcgctc gtcgagctct
     7381 cggccgaagc gatcctcatc taccaggacg gcatcgtcac ctacatcaac cccgccggcg
     7441 tgaggcttct tggcgcgcaa gaccccggcg aggtcatcgg gacgaacgtc gccgatatca
     7501 tccatcccgg ctcccgcgag accgtcagat ccttcgctgc ccggaacctg cagagggggg
     7561 agacgccgct cgtcgagctc ccggtcgtca ggcttgacgg gacgatggtc ttcgtcgagg
     7621 ggcggggcac ccgggctttc tttgcgggga ggcctgcagt ccaggtcgtc atgcgggata
     7681 tcacccaccg aaagcaggcg gaagagcggt tgcaggcaag caatcgccat ctcctgctgc
     7741 tcaacaggat catcggcata tccgcccagg ctcaaacccc cgatgaactc ctggagacgg
     7801 cgcttgacca gacgctcgac ctcctcggct acgatggcgg cggcatctac ctcaccgaac
     7861 ccggaaggag cggggtcgca cttcgttacg aacggggtat gccgaagaga tgtctggaac
     7921 gggccgggga actcttcagg gcatcgtacg ccgaagtatg cgccaccggc gctccccgtt
     7981 acatcgagca ggacgatagt ccggtatcct gtctcctccg cgatatcggg tttaccgcac
     8041 tggcctgcac cccgttcacc gtcgagcccg acgttgccgg agcgcttctt gtcgggagca
     8101 ggaaccgaca gtccttccct cccgaagagc gtgcactcct cgaggcggtc ggccgggaga
     8161 tcggggccgg gatgctccgg ggaatgctcc accagagact cgaggcggcg aaccgggagg
     8221 caaatctcta cctcgatatc ctcacccacg acatcaagaa cgccgataac gtcgcgaaca
     8281 tatacgccga cctcctcatc gacgaactcg agggggaagt ggtcgggcat gctcagaaac
     8341 tgaaggacgg tatccgcaaa agcatcgaga tcacggcaaa cgtcgccacc atcagaaaga
     8401 tccacgagac ccgggccggg cttgaacctg tcgatctcga tgccgtcatc aaaagcgaga
     8461 tcgagcactt ccccgatctc cctatctgct acgacgggct gcccctgaag gtcttcgccg
     8521 acgatcttct cccccaggtc ttcacgaacc tgatcggcaa caccacaaag cacggcggat
     8581 caaaggtcaa tgtcgccgtg gcggtcgagg acctgggcga agaggtcgtc gtcaccgtcg
     8641 ccgacaccgg ccccggtata ccggatgaca tgaaggagac gatcttatac cggttcgagc
     8701 gggagaacga caaaaggggg agtcagggcc tcggtctctc gatctgccgg atgctcaccg
     8761 cccgttacgg cggcaggatc tggatcgagg accgggtggc ggggcgcccc ggggagggtg
     8821 cggcgttccg gttcaccctc cggaaggcgt gagcctctca ggtccgaccg cttatctctc
     8881 cgccatcgcc tgcaggatcg acatgatacc gggtctggcc ctcctccgct gcctcgaacc
     8941 gaagtcgccc atctccatgt cggtggagtc gtacagttct ttattcaccc ggtcggtgat
     9001 ctcgtcgaat attcgccggt acgcggtgca gtgggggtcg acgccgcgga tctcgccgtc
     9061 tgtcggcgct atcgcgttgt aggggcaccc accccggcag tactttatat ggggacatcc
     9121 tttgcattcg cggtcgacgt actccctgaa ctcctgcatg agcctccagg catccgaccg
     9181 ggcgaggtct tccgggcccg ggcggtcgta gacgttgccc atcacgtatt ccggcatccc
     9241 gacgaaacgg tagcatgggt atatgctccc gtccggcccg atggcaaggg tggtgcccat
     9301 gcagtccacg aacgtgcaga cggtgccgtg ccgggtaaag acgcccctgc agatgtcgtt
     9361 gatgttcatg acctcgatac ggcccaggtt ctccaggtac ctgtcgagga ggtataccag
     9421 cagctctccg taagcctcag gttcgagcgc ccactctttt gggttctcgc tccggaggga
     9481 cggcagcgcc gggtgcagtt tgagggggaa cccgttctgc aggaagaagt tgaagatctc
     9541 ttccttgcgc tcgaccgacc ggttggtgaa cgtgcagatg aacctgacct caagcccgtt
     9601 tgcccgcgcg atatcgtagc cccgcatggt cttcttgaag tatcccttcc ccctctgcac
     9661 gtcgttgatc tcctcggggc cgtcgatgct cgaaccgatg gggatcctgt actccgcaag
     9721 tatccgggcc atatcggggg tgagcagcca gaggttgctc tggagggcaa acgtcggggt
     9781 aaggtgggcg agcccctccg agagtatggg gagcgcctgc cggtagaact ccgcgccggc
     9841 aaggagcggt tctcccccgt ggaaggtgat ggtgatctgg ttggactggt agcctttgag
     9901 ccactccgtt atctcccgga ccgtctcgat gctcattctt ggagaacgtt catccgagct
     9961 ccagcagtag tggcatttcg aggggcaacc gagcgtgggg atgatcatga cgtgaaaagg
    10021 gctcttcatc gtatcgagat tctccttgag agtgttaaaa cctactcata cggtcatccc
    10081 ggcagataga gctcccccat tccggtattc accgggctgc ctgcgccctg cggcacccgc
    10141 gagagtatcc tccgggctgc tcccggaggt tcttccccgg tgccgcagct gctcccccgc
    10201 gtgcgcaggg ataaaacgga gggactatcg gggggagtgg ggaagactgc gctnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcgtgcg cagggataaa acggagggac
    10381 tatcgggggg agtggggaag actgcgctcc cccgcgtgcg tgaggataaa actcgggcgg
    10441 gcacccggtg gggggtgtgc tctgtgctgc ccgcccgggc gaagttctac cggcaggcgc
    10501 cgggtcccgc atgacggtct ccgcaaccct tttcatgcaa ctcaccgtaa gaatgcatga
    10561 ggaagaagaa tggacttttc cgaatgcgtg aaattcgcaa acgaaaaccc tgtgacctac
    10621 atcgcgacga tggacggcga ccagccccgg gtccgggcgt ttgtcatgtg gtttgccgat
    10681 gcgaccgggt tctactacca caccgggacc ccgaaggccg tctggcggca acttgcgaag
    10741 aacccgaagg tcgagctctg cttctatgcc cccggcgacg tgaacacgat gatgcgggtc
    10801 gcgggcgcgg tcgagttcct cgacgacgcg gccttgaagg agcgcctcgt cggggatcgg
    10861 ccgtggctcc tccagatcgg catctccggc cccgccgatc cgaaactcgc cgtcttccgc
    10921 gtggcccacg gcgaggcgta tttctggacg atggagcaca acatgcggga ggcggacgct
    10981 ccccgggtcc ggttctaagg gacctctcac caatctttct tcatcgcctc ggccttcagg
    11041 tctttcggca tcagctcgat ggcgtagcgg agcgccgtcc ggggcatgac ccgcttgttt
    11101 ctcatgacgt aggagaagac ctcgtccgtg tgttcgcggc tcgcctcctt gaggagccag
    11161 ccgtagcctt tctgcaccag gtcgtccgtg tcggcaagga gaagatcggc tatggcgatg
    11221 gcttcgtcaa ggaactcccc gtgctttgcc ggcacgatca gcgagaccgc cgccgcccgg
    11281 cgcatccagc ggttatccga ctgcgcccag cgcttcagct cctcgatatg ctccgggtac
    11341 cggacgacga gatccccgac ggcatggttg cagaggccgt cgcatgccgc ccagttcgtg
    11401 atgtaagtgt cgatccagtg ccggaagacg gcgatatcgc cgggttcaaa gcgttcgccg
    11461 agcatctggg cccactggga gaccacgaac gcctcctcca tcatccctga ccggtagagc
    11521 tcctcgcaga gtccgaagat ctcctgcttc tcgcgagacc tgaccttctt ccagtacttc
    11581 tttgcgaccg taactgcggt ggcggtcttc atcccgtagc accggatctc ctccttgaag
    11641 aaccgctgac tgctcttcct gcgatcgggg tccgcgtgcg ccgcaaactc ctgccgtatc
    11701 gcctcgatga ccgggtccat agagaggtta cggagaggcg gcggataaac gatccggtag
    11761 agatagggct gtaacggggc aatctctccc ggcgaatatg ttcccggccg cgggcctcct
    11821 cgctcacgca gacgcgaacc ggacgcccgg gggaaggcgc gggcggcgta gtgcgggacc
    11881 tggatcccgg ggtgagccga tcagcgatac cctatgcgct tcaggagatc ttcggaggat
    11941 gccctctgct ggcgccgctt catgtccccc cagatccgac gctgcttgta gagccagaag
    12001 tcgaggtcgt cggggtcttc tgcgtagatg atacgatgcc gctcgagtat ccctcccctg
    12061 aggtaccagg ggatctcgtc gtaaacgacg atatcgtagt gctccggggc gatgagcatc
    12121 ccgtcatgca ccgcagtcct gtccgcctca ccggttaaga taaggcagat atcgatatcg
    12181 gacgttgggc ccgcgtagcc ggcgactcgc gaaccgaaga gcaggatagc ccggatcgaa
    12241 gggagaaggt cccggaagtg gttcaggatc tcgtcctgtt cgcattcaac catgtgagca
    12301 cctcgcctga aaacctgcgg agagacggca tcagccgctg cattgcgcca tacgcgatat
    12361 ggtcgttgat gccgtcatat tcgtgaacca ggcggtttct aagcccgttt gcagctttga
    12421 gatctcttcc cgtctcccgg ctgatgatcc cttcgcccac cagatagtcg agattggtgt
    12481 aatcatcctg tgccgaagag aggagtatcc gccggatgag ggcacagata tccgtagctg
    12541 attctacggc ctcctgaaac tctttgtaga gcgctttgcg caggatacgg tcgttcatca
    12601 cggcatccga gaggtggtgc tcgatgaacg cgatccgatc gatgagcacc tcaacctttt
    12661 gcagtatccg ctccctgacc aggtcatcca ttgaagagtt attcatccat tacctcgata
    12721 aaacttattc ccttctctac tgccgtgcgc cctccggccg gctgaaaacg aggtgctgcc
    12781 gggagaggtc atcccggccg cagccgggga tcacggctca gggcgcccct gcctcccgag
    12841 gtagcgctcg atgtgcgtct tttttatccc catcgcgagc agccgcccgg cgacctcctc
    12901 ccgcccgagc accgcgactt cgtcctttgc ctgccgcagc gcctcgatct cggcccggta
    12961 cgggagttcc cccggccccc cggtcaggcg ccggcgttct tctgcatcgc acccggtcgc
    13021 ggcgaccccc tcccccgggc tgagactgat ccggcacgcc ccggcgacgt acgcgatccg
    13081 ccgccagccc ccctcccact cgatcgccgc ataggatgcc ggggagcagt cgagggcgac
    13141 ataggccttc ccggcgatcc cggcaaccat catgccgtag tggccggtga cgatgcacgc
    13201 tgcggggaga tcgaagagcc agacccggtc ggcgagcggt gcgtgggcca ccacgatatc
    13261 gaccggccct tcggccgagc gggccatcct cctctccttt cctgtgggga cgccgaggaa
    13321 acggatgccg cggacggtct ccgccttccc cgacacttcg tggaggaagg ggcttgcctc
    13381 cgccgcctcc cggacgcggt cgtaggtgcc gcgcgcattg tcgttgttcc cctcgacgac
    13441 gatgcaatgc ctccccgccg ctgcaatatc ccggagaagg tcgacgaaga cctccgtctc
    13501 gtcgagcccc gagcgcgaac accggtcgta ggcggcatcg ccggcaaaga tgacgattgc
    13561 cgggtccgct tcatcgacga tgcgcatgaa cgaggcgatg ccgacccggc cgccgccagg
    13621 caaaccccgt tccctcgtcc cccacgcaag atcgctgaaa gcgatgatcc ccgtcacgga
    13681 ccggggttgg gcacccgggg gtgatagacc tcgcggtcgg gcgggaggtg tcgcgccgcc
    13741 ctttgcgccc cggccgcctt ccccgtagac agtatctcct ccacccccgt tggagtcggg
    13801 accgcctgcc gtttcaccgg atacctccgg acaaggtata aatacgcaca aatagagtct
    13861 ccatcaaggg attatcgtac ccgtttcatc cagcggacag aaacgaggag tgatttatga
    13921 caaacaaaaa gagactgacc accgctgccg gcgcacccgt gccggacaac cagaatgccg
    13981 tgaccgccgg tccacgcggc cccatgctga tgcaggatgt ctggtaccag gagaaactcg
    14041 cccacttcaa ccgggaggtg atcccggaac ggcgcatgca cgccaagggg tccggcgcct
    14101 tcggtacgtt caccgtcacc cacgacatca cccggttcac cagggccagg ctcttctccg
    14161 atatcggcaa gaagaccgac gtcttcatgc gcttctcgac ggttgccggc gaacgcggtg
    14221 cggccgatgc cgagcgcgac atccgcggct ttgccgtgaa gttctacacc gaggagggaa
    14281 actgggatct ggtcgggaac aacacccccg tcttcttcat acgtgacccc cacaagttcc
    14341 ccgacctcaa ccgtgccgtc aagcgcgacc cccacaccaa catgcgctcc gcgaccaaca
    14401 actgggactt ctggacatcc cttcccgaat ccctgcacca ggtgaccatc accatgagcg
    14461 atcgcgggat tcctctctcc tatcgccaca tgcacgggtt cgggagccac acgttcagca
    14521 tgatcaacgc ggaaaacgaa cgtgtctggg tcaaattcca cctccagacc cggcagggca
    14581 tcaagaacct gaccgaccag gaagccgagg cgatcgtcgc aaaagaccgc gagagccatc
    14641 agcgcgacct cttcgagagc atccggaagg gcgacttccc ccgctggacg atgtacatcc
    14701 aggtgatgac cgaagaacag gcaaaggcca tgccctacaa ccccttcgac ctcaccaaag
    14761 tctggtacaa gggtgagttc cccctgatcg aggtgggcgt gctcgagctg aaccggaacc
    14821 ccgagaacta cttccaggac gtggagcagg cggctttcaa cccggccgcc gtggtgccgg
    14881 gcatcgggtt ctcgcccgat aagatgctgc aggggcgcct ctttgcctac ggagacgcac
    14941 agcgctaccg cctgggcgtc aaccaccacc agatcccggt caacgccgcg aagtgtgtcg
    15001 cgaacccctc tcaccgcgac ggtcagatgc gggtgaacgg caacgccggc gccaccatcg
    15061 gctacgagcc caacagtttc ggcgaatggc aggaacagcc cgaatacaga gaaccgttcc
    15121 tcgctctctc cggtgcggga gggtcctgga acttccgcga agacgatgcc gactattaca
    15181 cccagccggg caaactcttc cgcctgatga gcccgggcca gcagcaggtc ctcttcgaga
    15241 acaccgcgcg cgccatggga gatgcgcccg aactcatcaa ggtccggcac atcgcaaact
    15301 gcctgaaggc cgatccggcc tacgggaagg gcgtcgcgga tgcgctcggc atcccgctcg
    15361 atcgcgtgaa gtaacccccc gtatggggcc actcccatca aaagggcggc aggagttcaa
    15421 tccccgaacg tgaactccgc cgcccagtca aacccttcgt tctcctcgta ccactgcggc
    15481 cagtgggagg cccatgccgg caccttcgcg tccctgaccc gctcgttgca cgggaggtag
    15541 ggcttgatgt cgaggaccgg cgtgccgtcg tcggcatcga tatacgcaac cctgatgatg
    15601 cccgcggcgg catcgacgcc gagcaccggg gcaaccgaga gcgcgatagg gttaggccgg
    15661 accggcgacc gggtggcgaa tatgccgatc gtatccgggc ctctcgtgta cggcttttcg
    15721 caggtcgtct cgctccggca ctctaccgta tcgaccccgt cgcaccacca gaggacgagc
    15781 atgtgactga accccgagag ttccgtaaga gcgggccgga acggttcggc aatctcgatc
    15841 gagaagaccc ctctgccggc atgcacggtg ccgaccggtg ttacgctgaa tctccgtgcg
    15901 tccatacagg aacggtgggc gcggcaggag aaaaagggcg cgagtctctc ccgcgggaga
    15961 ggtcacggga ggtatccgtc cacgcaaccg aggaaccgct ccacggttgc cgtcgagtat
    16021 ggcccttcct cgtaggcggt cgtgaccgtc aggcggccgc aaaaggtcga ggccgccatc
    16081 aggaacccgt acggccagca gacgcaggga aggtactgga tatcgagaag gtcgagcgct
    16141 ctgccgtctt tccccgggac ggggaggtag tcgccggggt caaagacacc gaggttcgag
    16201 aagaccgggt tcttgttgcc cgacgaggtg tatcgcccgg ccatctcatc gaagaacgcc
    16261 cgcaccgccg gcatcccgcc cgccatgata ccctcgtaga agaggatggc ggcaagtccg
    16321 gggttccctg ccttcttatg ggccgttatc gccgtcaccc ggccgatgat atcttccagc
    16381 cccgcgccct cctccacggt gagggtgaca tcataggcga ccgagaggtt cacgggcagc
    16441 ccctcgccgt ggcatcccgg gtatctcctc cggaggtcgg ccgacgtcag gatcgaccgg
    16501 ggcgccccac ggtccgtggg gtcgtcccgg gtatccagca gggcgaggaa gaacgcaccg
    16561 atcaggacgt cgttcaccgt ggccccgtgc tccctgccga atgccttcat gcgctcgagg
    16621 cgatccggcg caagcgtccg tgaggcgatc cgcggcgttc cccgtcccat ccgcccggcg
    16681 gggaaacgcc accggtcgat gaacggttcc tcctctgcga gcgcccgtct ttgctcctcg
    16741 tcggaggaga gcgcgagtat ccgatccgtg ctccggtcgt agggctcgcg gggagggggc
    16801 cggtagcccg ggtcggccac gatgccccgg taggtagcaa agatgtcgcg ggcaagggcc
    16861 atggtgccgg agacatcgca gaaaccgtgg tggcaggtga cggcaacgat atcccctccc
    16921 gccctccggt agagaccgac ccgcacctgc ggcccggcgc ggacatcgag cggcggcggc
    16981 ggcatatcta catccccgcc agccgggaga acgacgaacg ccctcgccca ctcctcctcc
    17041 gcgatctctt cccagacggg ccggccgtca acctccgcgt accgggacct ggtgtagggg
    17101 tcggacgcaa ccgtcctcat cgtcgccttc cgcagggccc cggcatcgat cccgccgtca
    17161 aacgtgaaga cggcgtgcat ggtgggatcg tagatacgct cgaagtagac gttgaagacg
    17221 tcgaaggcgg gtgccgggta acggggagac atactgtaag agtctccccg ccgggataac
    17281 tgccttatgc tcgatagtgg ccggaacggc cgcgatcagt aggcggtcca cccggcgtcg
    17341 gcggtgatga ccgccccgtt caccaggctg gcctcatccg aggccaggaa gagcgccacc
    17401 cgggcgacct ctatcggctc tcccatacgc gggttggttg ccatacccgc catatagacc
    17461 tccgtcccct cccggctcag gctcttgccg gtgagtatat cggtgctgac gccgcccgga
    17521 gcgatgacgt tcgaccggat gcccttcttc gcatacgcat aggcgacatt catcgcaagc
    17581 ccgacgaccc cgtgcttcga cgccgtgtat gcggccccgg cccttgctcc gcgtatgccg
    17641 cccacggatg ctatggtgac gaaagcaccc cctcccgcct tctccatctc cggaacggcg
    17701 ccccggaaga ggcgcatcgg cccggtcagg ttgacgtcga cgaccgcgtt ccacgtatcg
    17761 tcatcgagct cggcgaccgg ttccatcctg tccatcaacc cggcgttgtt gacgacgaca
    17821 tcgagtttac caaacgtctc tactgccgcc cgtatggcat tccggatatc ttcttcgcgg
    17881 gtaacatccc cggcgaccga aacggccttc ccgccggcgg cggttatctg atcgacgagc
    17941 gccgccagtt tatcctccct tcgtgcaacc gccacgacgg atgcgccttc cccggcaaag
    18001 agccgggcaa tctccagtcc tatccccgaa ctggctcccg tgacgagaac aactttatct
    18061 ttcaatctca tggtttcccc ggtatccggg gagcgcggta tgacatccta aatgtttcgg
    18121 cacgcggcgg acatggccgc cggtctgctc cgggaagcga tgacggcgct gccggcgtgc
    18181 tacaaaggta tttgagatgg tggcaaaaag gtaccataag tatcaggaag aacaggatac
    18241 agcat
//
LOCUS       DAOSII010000026        18084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_3324_length_18084_cov_55.694437, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000026 DAOSII010000000
VERSION     DAOSII010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 18084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18084
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3324_length_18084_cov_55.694437"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..301)
                     /gene="rrf"
                     /locus_tag="PLV90_03385"
     rRNA            complement(180..301)
                     /gene="rrf"
                     /locus_tag="PLV90_03385"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(307..378)
                     /locus_tag="PLV90_03390"
     tRNA            complement(307..378)
                     /locus_tag="PLV90_03390"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(344..346),aa:Cys,seq:gca)
     gene            complement(488..3416)
                     /locus_tag="PLV90_03395"
     rRNA            complement(488..3416)
                     /locus_tag="PLV90_03395"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02540"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02540"
     gene            complement(3541..3613)
                     /locus_tag="PLV90_03400"
     tRNA            complement(3541..3613)
                     /locus_tag="PLV90_03400"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3578..3580),aa:Ala,seq:tgc)
     gene            complement(3680..5146)
                     /locus_tag="PLV90_03405"
     rRNA            complement(3680..5146)
                     /locus_tag="PLV90_03405"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01959"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01959"
     gene            complement(6191..6931)
                     /locus_tag="PLV90_03410"
     CDS             complement(6191..6931)
                     /locus_tag="PLV90_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOF96190.1"
                     /translation="MDEEPVEILAGMAERGEIDPWNVDIVDVTDRFLAELDRRKELDL
                     RVSGRTLFYAACLLRLKSEYLEGWSEEEDDESALDEEEDGFPGIDFDFGTDDGTEPIE
                     RLEREIQRRLGRKNLRKRPPVTLYELIKQLKTAEKEQRRRQRRRMPAEREPDLDISAG
                     DVVAVAHDEGYQKAVFVVMEEFRRASRDGDTLTLDGLSASLGRTRREVYIPLLFLMLE
                     GRLALWQDEFFGEVYIGDRIPEGDTDEE"
     gene            complement(6924..10367)
                     /gene="smc"
                     /locus_tag="PLV90_03415"
     CDS             complement(6924..10367)
                     /gene="smc"
                     /locus_tag="PLV90_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOF96191.1"
                     /translation="MYITQLEIDNFKSFAKKTKIPFFGGFTVISGPNGSGKSNIIDSI
                     LFVLALSGARGLRAEKLTDLINVNTGKNTAEVTATFSDGTAIRRRIKRTPAGYYSYNY
                     LNNRLCKQSEVIEFLAKIGIKPEGYNVVMQGDITRIMEMSDGERRKIVDEIAGVAEFD
                     HKREQAFSELEVVRERIEREEILLSELVARLDALQHEREQAMEYRRWQEELEHLGRCR
                     GAALVRQKEQEIGTLHALTLDQKEALGRTAGEADAVRAQAEEARERRQAVEEEISTKS
                     GPEYLALVGRLEEARGGIKLAEKTIERLAAGRDENLETIQRVYMDGRRAEAKVEGCAG
                     QIRTLSIDRANLAMEASIERDQLAAVEKRIASESKEVEGIKDQLFARMQDLENRKELR
                     AKILREQDILIEKSRMRTSERERLEARTRQIDEELVNKRAQMADYASSMATCETEKRR
                     IERDLSEAESTLFARRSALDRLQKEIRENQQSLMRLEAQQQAQGDAGGKAMDAILGME
                     GVHGTVAQLGRAPPEYATALDVAAAGRLRWVIVDTDGIASDAIRYLKDNRLGRVTFLP
                     LNKLRPPVLSPLEADPGIVGYAVDLLEFDPAFERAFRVVFGATVVVDTLERARRLMGR
                     YRMVTLEGDFVEKTGAMTGGSQAKKVRGFGVAVDDEVARIRAVLADLEAEAAEIEASI
                     RRFAAAAEAKRSERSAVDEQVARYRMLAEEFSKRTDVLAGEKESLAKTLEGMLLEAKA
                     GGEELARLEADRERVSAEVAGLSAEVDALKKKLDDTEIPALTEQYERHRRSVEDIERR
                     LRNKDADITDAKRERQHFANRAEELAAERDRLTAKNQEIDGEIAATTEQIEGLRRLTV
                     QLEARQKEFSNELAGLHGERDRILEEIRALDRQALDLSGAMEKARMQIDALMERERIL
                     IEELTALREQAGDVESDLDLAAIDAGIAEAERALKKIGAVNMLAIDECDRVGARVEER
                     TAKKEVLSRERMMLLERIERYEKMKYEAFMTAFTAIDTNFREIFARLTEGSGRLVLEN
                     EDDPFAGGMTFAVQPRDKKVHLLSALSGGEKSLTTLAFIFSIQQYMPAPFYALDEVDM
                     FLDGNNVGRIAAMISDLSGNAQSIIVSLRKPMIESADRIVGVTIRPDKSTYVTGVQNN
                     G"
     gene            complement(10385..11002)
                     /locus_tag="PLV90_03420"
     CDS             complement(10385..11002)
                     /locus_tag="PLV90_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96192.1"
                     /translation="MAQKDARIRFLERELAEREKEMETMTEPEQPAAPAVNDERLRDL
                     ERKVRELEALVRGLTEEILDLKSVTMKLSREAEERRVKSVTAESKKAGATLQAEPRAA
                     VEPRVAAVPREPAQPAEKQPVRANQDDADMELIMQNDGTLKPESRRSSEYIVASNKFG
                     NAPMKGQSRSRGGKSSDRTLFVEQKKREVENVIQAEEDDTVDLDR"
     gene            11191..12462
                     /gene="glyA"
                     /locus_tag="PLV90_03425"
     CDS             11191..12462
                     /gene="glyA"
                     /locus_tag="PLV90_03425"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOF96193.1"
                     /translation="MSYLANFDPEVAGIIENERLRQINGLELIASENVVSKAVLEATG
                     SIMTNKYAEGYPGKRYYGGCEYHDAVENLARDRLCKLFGAEHANVQPHSGSQANQAVY
                     FAYLNYKDRIMSQSLTQGGHLSHGSPVNITGRWYSIFHYGVDSVTETLDYAVIEDMAR
                     IAKPQIIVCGASAYPREIDFKAFQEIADTVGARCMADIAHIAGLCATGYHNSPVGVVD
                     IVTTTTHKTLRGPRGGAIMCGKENAAAIDKSIFPGMQGGPLMHIIAAKAVCFKEALTP
                     AYGDYCGQVVRNARTMADVLTEEGLDLVSGGTDNHLILLDLTGVSTNHEHLTGLAAET
                     ALGEAGITVNKNTIPREQLSPFVTSGLRIGTPAVTSRGMKEEEMKQIGHWIARVVKDV
                     ARDRTSKKAITEVREEVIALASKYPLYPEVA"
     gene            12459..13310
                     /gene="folD"
                     /locus_tag="PLV90_03430"
     CDS             12459..13310
                     /gene="folD"
                     /locus_tag="PLV90_03430"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOF96194.1"
                     /translation="MILDGKAVSEKRLEILKEKIDESGLYPRLATVIVGEDPASQMYV
                     RMKHRACERVGIGSIGIELSADASTEQVLEAVARLNTDPDIDGILVQLPLPRGVETTR
                     VIDAVAPIKDVDGFHPFNLGRLLAGKPVYAPCTPQGVMTILDEYGIPIAGKRAVVVGR
                     SIDVGRPMAVLLLNADATVTICHSRTENLADETRRADILVSAVGKAKFVRPDMVKAGA
                     TVIDVGINQDEQGRLSGDVDFEAVRERAGAITPVPGGVGPMTIATLMENTFKAARLRT
                     CCDEIVR"
     gene            13286..14101
                     /gene="folP"
                     /locus_tag="PLV90_03435"
     CDS             13286..14101
                     /gene="folP"
                     /locus_tag="PLV90_03435"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOF96195.1"
                     /translation="MLRRDCTINRLRIGGDAPVRLMGVINCSPESFYRGSYTPAGRVY
                     DRALAMIDAGADLIDLGARSTAPGSPPITVAEEAARVEAALSELDGTGIAVSVDTRYA
                     EVLDLALRHDIHAVNDISGLADERYARAVADSGLPVFVMASLREPGDAVGLAATLAAL
                     DAVVARCTRLGIEEYVLDPAVGRWVPGRTSEDDWGLCRNFEAFSSFGRPVLAAVSRKT
                     FIGDLLGREPDDRLAGTLALTAMLVEKGAAVVRSHDVRETADLLRVSAKMRGR"
     gene            14098..14904
                     /gene="cofE"
                     /locus_tag="PLV90_03440"
     CDS             14098..14904
                     /gene="cofE"
                     /locus_tag="PLV90_03440"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOF96196.1"
                     /translation="MTAPSFSVYGLATALIRAGDDVAAHLIEAAARAGCEGFQTGDVV
                     VIAESAVATAEGRAVQLAGIEPSDEARRLAENYRMDPCQVEVVLRESDRVVGGIPGFL
                     LCMKNGTLLPNAGIDASNTPRGSVLLLPADPDASAARIRATIRERSGADVGVIVIDSR
                     THAMRLGCSGVAIGCSGIPSVLDERGKQDLFGRELEVTKRAVADCIASAAELVMGEAN
                     ECVPAAVVRGLMLPVGDYTGVATIDASECLFMGVALHADPSLLVDDKREP"
     gene            complement(14830..15477)
                     /gene="cofC"
                     /locus_tag="PLV90_03445"
     CDS             complement(14830..15477)
                     /gene="cofC"
                     /locus_tag="PLV90_03445"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HOF96197.1"
                     /translation="MYFHALIPFKPVNPKTRLSCILDQEEREAFARAMLEDVIASVLK
                     SGCSATLLCTHPFKHENALIAVRKESLNDAINWALGQFHCPALIIMADIPLVTAGDIQ
                     RLIRTEKDMAIVPGRGGGTNIIFLKKPRCFRADYYGASFLDHMRIAGECGFSVEVIDS
                     FRMSTDIDEKEDLVEILVHGKGRKSREYLENSGFSLVIDEKGRVGVQRDAHKETL"
     gene            complement(15599..15829)
                     /locus_tag="PLV90_03450"
     CDS             complement(15599..15829)
                     /locus_tag="PLV90_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96198.1"
                     /translation="MPFDELRNTVAKYLKTAQDVEEFRITLAVLESGAWRLNLSWIEK
                     IEDFEWPRRAGMKVNANTGALLEFRYSYNYSH"
     gene            16009..17004
                     /gene="cofG"
                     /locus_tag="PLV90_03455"
     CDS             16009..17004
                     /gene="cofG"
                     /locus_tag="PLV90_03455"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HOF96199.1"
                     /translation="MHRRVITFSRNAFLPLTTVCRNRCGYCCFRTPVREGCVMPPDDA
                     LRTMEASARLGCTEALFTFGERPGAVAGFDAVLGRIGYADILDYVYDLSLAAIERGLL
                     PHTNAGILSYDELDRLRGVNASMGLMLETTADIPAHRNSPGKDPAVRIDMIENAGRLS
                     IPFTTGILVGIGETMEDREESLSVIRDLHVRFGHIQEVIVQNFCPKPGTLMEGAPAPG
                     TDELCATIALAREVLPADVAVQIPPNLADASHLVGCGVDDLGGVSPLTIDYVNPERPW
                     PQLEELRRVAGDAELRERLCIYPQYIEKGWYPPLLEPLIRRLAARIAAPGGGEGA"
     gene            17047..17772
                     /locus_tag="PLV90_03460"
     CDS             17047..17772
                     /locus_tag="PLV90_03460"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOF96200.1"
                     /translation="MKQVDLLYSGKAKSVYRTDDPGVYIMKFRDDITAFDGGKKDTLG
                     GKGRYNAEVSSFFFRYLEENGIKTHYLSSVDPTAIAVRSLTMIPLEVIVRNVAAGSIV
                     RNYPFREGEVLDPAVIVIDYKSDAHHDPMLNDDLIYALGLATPEELDQIKAMALAVNI
                     VLSEYLAARGITLVDFKLEFGKTDGEIVVGDEISMDSMRLWDKETGASLDKDVYRFNK
                     GDVMETYAGVAKRILSPPGEESA"
BASE COUNT         3376 a         5869 c         5130 g         3709 t
ORIGIN      
        1 ggtgcatgaa gagaaaaccc ggtgcctgga gagaaagggt cgctttgagg cgccgtccgg
       61 atgaaacgcg accgtccggt aaggatggaa caagtatcaa ggcgcgttgc aggagagaag
      121 taaaggcaaa cgctcgcaaa atgtcaatca gaaaagcaat cattcaatgt aaagggaggt
      181 aatagcagcg aattgggctt cccgaggact ctcgtacctc agtaccacac agcacgtgag
      241 agggcttaac ttctgggttc ggaatgggtc caggtgtttc ccctctgcta tggccgctat
      301 taccaaagcc aaggcccgga tttgaaccgg ggtgtagttg atctgcagtc aaccgcgtgg
      361 ccgctccgcc accttggcaa gccgtgttac ctcctatagg ttagtctccc gggcatataa
      421 aggtaatcac cttgcacccg gatgaaacca tcaggattaa gtgcgcgatt ctggatttcg
      481 tctgagttca gcggaaggca tcggacgtta gtatccgcgg actgaacatg tcgttgcctt
      541 catgcttaca tcccggacct atcaagcggg tcttttaccc atgtcctcaa cggagtctct
      601 tttcaggtca ggcttcaagc ttagatgctt tcagctttta tcccttatcg cgtagctgct
      661 cggcattgcc ctgtcggaca accgatcgac cagaggcgac gacggaaagt tcctctcgta
      721 ctatttccat cttaccttca gactccaaac actccagata gatagtaacc gacctgtctc
      781 acgacggtct aaacccagct cacgatcccc tttaataggc gaacaacctc acccttggcc
      841 gctgctgcac ggccaggatg gaaagaaccg acatcgaggt agcaagccgc cgggtcgata
      901 tgtgctcttg ccggcgacga ctctgttatc cccggggtag cttttctgtc gtcaatggcc
      961 ctcatcaata aggctcattg gttcgttaga cccgagtttc ctctcgcgat tacttgcttt
     1021 gcgtaatcgc gtcaggccaa cttatgctct tgacactctc ctgtgagttt ctgactcaca
     1081 tgagttgacc atagggcacc cttgatattt tttcgagggt gtggcgcccc acccaaactg
     1141 cctacctacc gttgtcctca agtttgagtt agtgttacag taaaccaagg atggtgtctc
     1201 attggtggct ccccaccccc cggaggggat ggttcgacgc ctcccatcta cgctgcgcaa
     1261 ggaataccgt aacacagcga caggctgcag taaagctcca cggggtcttc acttcccatc
     1321 tggagtccct agtctctgca ctaggatgaa atgttcaacg gattcgtgtt agggacagta
     1381 gggctcttgt taatccattc atgcaagtcg ccaattaagc gacaaggtac tacgctacct
     1441 taagagggtc atagttaccc ccgccgttta cgagtccttc gtccggttga acccggtatt
     1501 cagatactcg cactgggcag gaatcagtga ctatactaat cgtttccgag ttgcagtcac
     1561 ctatgttgtt attagacagt cagagccccc tggtcactgc gacctgcctg atcgccaggc
     1621 aggcacccct tatcccaaag ttacggggcc aatttgccga gttcccttaa cacgattaaa
     1681 ccgacacgcc ttagcctctt cagctagggg cacctgtgtc agatctcggt acggtcattc
     1741 atcccccttt tcacgggctc caggaatttg cctggttacc ccatcacgcc ttcactcgct
     1801 tctcaccatg gcggtactcc acgagcttga acgcttggac agggcgacga ccctgtcagg
     1861 catatcccga agcgtcaggg cgaactaacg ctgcggatga atggtacagg aatattaacc
     1921 tgtttccctg ttgatgtgct cgaattacga cacaccttag gaccgactaa ccctcggctg
     1981 acgaacattg ccgaggaaac ctagcccctc cggcggttgg gattctcacc caactttgct
     2041 tctactaatg ccagaattct cattactacc cggtccacag gagctcacgc ccctgcttcg
     2101 acccaagcag cacgcctctc tacgccatca cacaaacgtg tggtccgtgg tatctgtagt
     2161 aggctttagc cccgtccatt ttcagtgccc caaacctcga ctggtaagct gttacgcact
     2221 ttttaaagga tagctgcttc tgagctcacc ttccagttgt ctttggcctg ggacgccttt
     2281 cagtgttgac acttagccta cattgaggga cattaaccac ggtctgggtt gtctccctta
     2341 cggactacaa gcttaccccg tagtccggac tgccagccat cttcgacgac ggggaatttg
     2401 gagtttgaca ggagggtgag ggatttctcc cccagctccc ccaatcagtg ctctacctca
     2461 ccgtctatct ccggccaggt catgctacgg catgtttcga gaggaaccag cagatgccca
     2521 gctcgattaa tctttcactc ctatacgcag gtcacgcgaa tgatttgcat atcaaaaccg
     2581 cttgcggtcc tccacgcgcc tttcggcacg cttcaacctg cccacgcata gatcgctagg
     2641 cttcgggtct tatcctgcag actccacgca cttttaatac gtcgtcccat gcaagcgcta
     2701 cggacatgtt ggtttccctt cggctcccct tttgggttaa ccttcgcctg cagaataaac
     2761 tctctggccc gttcttcaaa acgtacgtta cgacaccggc aaccacgccc gtactaccgc
     2821 ctcgcgacgg gttccttcac gcggaagatc ctttcgcgcc gtaactcacc atcacctatc
     2881 agtttcaggc gcttttaacc acctttcaag ggttactttt cagccttcgc tcacgctact
     2941 aatacgctat cggtttcgag tagtatttag ctttggaggt tgatgacccc cagattcccg
     3001 cgagaattcc gacccgcggt actcaagact ctaccaggat atctcggctt atacgtacgg
     3061 gactatcacc ctctttggtg ccacgttcca gaggacttcc gtttgacctt gatatccgga
     3121 cggtaggctt actacaccac atctccccgt gaggggattc agtttgaact ctgtcgtgtt
     3181 cgatcgcctc tactaacgac atctcggttg atttctcttc ctccccctac taagatgttt
     3241 caattcgggg ggttcccgat ccttacggat caacaccgaa gtgttgggat gtcccattag
     3301 ggtatctccg gatcgtagac tccatgcgtc ttcccggagc ttatcgcagc ttggcacgcc
     3361 cttcttcggc actcgaaccg agccatccac tgataggcat atcgccgttc cgctgaactc
     3421 atttaacgtc cagaatcacg cacctttaca cggcttcttc aggtcaaaga ccttcagccc
     3481 ttccccggtg actctcatct ccgggtgcat tgaaggcagg aagggatgct tcctgcccag
     3541 tggactcagg gggatttgaa cccccggcct ccgcctcgca aaggcggcgc tcttccaact
     3601 gagctatgag cccgagttac ctgttgacat cggcaatttt acgttttgtc cagcaaccgc
     3661 atcccaagcg aatttcatta ggaggtgatc cagccgcaga ttcccctacg gctaccttgt
     3721 tacgacttaa ccccccttgc ggaacctaga ttcgactacg gcaacgaccg cagcctcatc
     3781 caaaccccac tcgggtggtt tgacgggcgg tgtgtgcaag gagcagggac acattcaccg
     3841 cgttattttg aaacgcgatt actacggatt ccagcttcat gtgggcgagt tacagcccac
     3901 aatccgaact aaggacaggt ttaggagatt gccttcacct ttcggtgtcg atacccattg
     3961 tcctgcccat tgtagcccgc gtgtagcccg gataattcgg ggcatgctga cctaccgttg
     4021 cccattcctt cctcctcttt agcagaggcg gtcccaacag tgtccccatc agtccggaga
     4081 ccatgctggc aactgttggc gtgggtctcg ctcgttgcct gacttaacag gatgcttcac
     4141 agtacgaact gacgacggcc atgcacctcc tctcagctag tcatgcagag tcttcagccc
     4201 ggcaatcatt cagctgtctt atccggtgag cttcccggcg ttgagtccaa ttaaaccgca
     4261 ggctccaccc gttgtggtgc tcccccgcca attcctttaa gtttcagcct tgcgaccgta
     4321 ctccccaggc ggcgcgtttc acggtttccc ttcggcacct cagtgacacg tggtcactga
     4381 cacacctaac gcgcatagtt tacagctggg actacccggg tatctaatcc ggtttgctcc
     4441 cccagctttc gtccctcact gtcgaagccg ttctggtgag gcgccttcgc cacaggtggt
     4501 ccctcgagga ttacaggatt tcactcctac ccccgaagta cccctcacct ctcccggttc
     4561 caagattgcc agtatccctt agacgcctga cggttgagcc gtcagatttc ccaagagact
     4621 taacaaccca gctacgaacg ctttaagccc aataaaagtg gccaccactc gagccgccgg
     4681 tattaccgcg gcggctggca ccggtcttgc ccggcccttt cttcaggtgt gttttagaca
     4741 cccgaacagc ctgcatgtac aggcactcga ggttccctta tcacggttgc ccgcattgta
     4801 aagttttcgc gcctgctgcg ccccgtaggg cctggattcg tgtctcagaa tccaactccg
     4861 ggctcttgct cccacaaccc gtaccgatta ctggcttgtt gggccgttac cccaacaaca
     4921 acctaatcgg ccgcagatcc atcctaaggc gccggagctt tgggttacag ggcattccag
     4981 catctgtaac ctatagggta ttatccccag tttcccgggg ttatcccctt ccttagggca
     5041 ggttatccac gtgttactga gcagtacgcc gagggccgaa ccctctcgac tcgcatggct
     5101 taatcgaacc ccgatagcag tgacctctgg caggatcaac cagaatttgg aagtatgcac
     5161 actactaaac ttgggaggaa ttagcggtta ctgaacaaat ttccgcattg ccaatgccaa
     5221 cgtcagaacc aacccgcgtc atgcgcggtc gtgtcggttc tccatcaaac aggaaaacaa
     5281 tgtttgttct gtaggtattt aaacatttgt ttcctgcatc gggtctgagt catgagaact
     5341 ccgaccttca tatccgtttt gatacaggga gtctacccgg acgggcctgg tatttcacct
     5401 gtggggaagt aacgtttgtt gcttctggca tataaacgtt agtattccac ttcaggttcc
     5461 attccggagt tctgagaaga acttccggcc cgactgcaga aactcgccgg aacagtgcgt
     5521 tccggcggcc ctgtttcgcc tgtgcaggaa gcaatgtttg tgagtcatca catataaaca
     5581 ttacttcttg cccgggtctc gggccggaag ttcatcgcgt attcccgacc cgatcgcacc
     5641 ggaatcccgc atcgcggaac ccggcgcatc ttgtgagaat taacgttgaa cttccggata
     5701 tataaacatt gtcaacctgt atcaggccca aatcacgact tgagccctca caatggttga
     5761 gggggagccg accagaccac ccgaaacggg ctgcacggtc cggcgccccg gcaacacagc
     5821 cttactatgt tggcgccaat gttatataaa catttccctg ctcagtcggg tccgagcacc
     5881 cggctcggac ccccatgaac gtcggaagcc tcgtgcatcc tgcacctgaa tgcaggatcc
     5941 ggcacgagaa tcccttccga cttgactatt acatgttgat gccggaggta tttatacatt
     6001 taccctgcac acgtgcacaa aaccggtatc cggccggtcg cccgccctgc gccgccggcc
     6061 gaatacggcg ccatatataa ccgtgatgcg tggcgctcca gggcgcgcgc agcagcgcgg
     6121 gccatccgtc cggcgggctg cacggaaaac agagcgatat ccggcaccta tcggcccgga
     6181 ttcgccagga tcactcctcg tcggtgtcgc cctcggggat gcggtcgccg atgtatacct
     6241 cgccgaagaa ctcgtcctgc cagagggcaa gcctcccctc cagcatcagg aagaggagcg
     6301 gtatatacac ctcgcgccgg gtccgcccca gcgatgcgga gaggccgtcg agcgtcaggg
     6361 tatctccatc gcgacttgcg cgccggaact cctccatcac gacaaagacc gccttctggt
     6421 agccctcgtc gtgcgccacc gccaccacat cgccggcgct gatatcaaga tccggttccc
     6481 gctctgccgg catcctccgg cgctgccggc ggcgctgctc cttctccgcc gtcttcaact
     6541 gtttgatcag ctcgtaaagt gtgaccggag gccgtttccg gaggtttttg cgccccaggc
     6601 ggcgctggat ctcgcgttcc aggcgttcta tgggttcggt cccgtcatcg gtgccgaagt
     6661 cgaagtcgat gcccggaaac ccatcctcct cctcatcgag agccgattca tcgtcttcct
     6721 cctcgctcca cccctcgagg tactccgact tcaggcgcag caggcacgcg gcgtaaaaga
     6781 gcgtcctccc cgagacgcgc aggtccagtt ccttccggcg gtcgagctcg gcgaggaacc
     6841 ggtccgtcac gtccacgata tcgacgttcc agggatcgat ctcgccccgc tcggccatgc
     6901 ccgccaggat ctcgaccggt tcctcatcca ttgttctgca cgcccgtcac gtaggtgctc
     6961 ttgtcggggc ggatcgtcac gcccacgatg cgatcggcgc tctcgatcat cggctttcgg
     7021 agcgagacga tgatcgactg cgcgttcccg gagaggtcgc tgatcatggc ggctatccgg
     7081 ccgacgttgt tcccatcgag gaacatatcc acctcgtcca gcgcgtagaa cggcgcgggc
     7141 atgtactgct ggatcgagaa gatgaacgcg agcgtggtga gcgacttctc gccccccgag
     7201 agcgcggaga ggaggtggac ctttttgtcg cggggctgca cggcaaacgt catgccgccg
     7261 gcaaacgggt cgtcctcatt ctcaaggacc aggcgcccgc tcccctccgt cagccgcgcg
     7321 aagatctccc ggaagttcgt atcgatcgcc gtgaacgccg tcatgaacgc ctcgtacttc
     7381 atcttctcat agcgctcgat ccgctccagc agcatcatcc gctcccgcga gagcacctcc
     7441 ttctttgccg tccgctcctc gacgcgcgca ccgacccggt cgcactcatc gatcgcgagc
     7501 atgttcaccg cgccgatctt cttcaacgcc cgctcggcct ccgcgatccc ggcatcgatc
     7561 gcggcaagat cgagatcgga ctccacgtcg ccggcctgct cgcggagcgc cgtaagttcc
     7621 tcgatgagga tgcgctcccg ctccatgaga gcgtcgatct gcatccgggc cttctccatc
     7681 gcacccgaga ggtcgagggc ctgcctgtcc agagcccgga tctcttcgag tatgcggtcc
     7741 cgttcgccat gcaggccggc aagctcgttt gagaactcct tctggcgggc ctcgagctgc
     7801 acggtcaggc ggcgcaggcc ctcgatctgc tccgtggtgg cggcgatctc gccgtcgatc
     7861 tcctggttct tcgccgtcag gcggtcccgc tccgcggcga gctcctcggc ccggttcgcg
     7921 aaatgctgcc gctcccgttt ggcgtcggtg atatccgcat ccttgttccg gagccggcgc
     7981 tcgatatcct cgaccgatcg ccggtgccgc tcgtactgct cggtgagcgc ggggatctcc
     8041 gtatcgtcca gtttcttctt gagggcgtcg acctccgcgg agaggcctgc gacctcggcg
     8101 gatacgcgct cgcggtccgc ctcgagccgg gcgagctcct cgccgccggc cttcgcctcg
     8161 aggagcatcc cctccagggt ctttgcgagg ctctcttttt cgccggcaag cacgtccgtg
     8221 cgcttcgaga actcttccgc gagcatgcgg tagcgtgcaa cctgctcatc gaccgcgctc
     8281 ctctccgagc gcttcgcctc ggcggccgcc gcaaagcggc gtatggacgc ctcgatctcc
     8341 gctgcctccg cctccagatc ggcaagcacc gcacggatcc gtgcgacctc gtcgtcgacc
     8401 gccaccccga acccccggac cttctttgcc tgggaaccgc cggtcatggc gccggtcttc
     8461 tcgacgaagt caccctcgag cgtgaccatc ctgtagcggc ccatcagccg ccgcgcccgc
     8521 tcgagggtat ccaccaccac cgtcgcgccg aagacgacgc ggaaggcgcg ctcgaacgcc
     8581 gggtcgaact caagaaggtc gactgcgtac ccgacgatgc ccgggtccgc ctcgagcggc
     8641 gagaggacgg gcgggcgaag tttgttgagc ggcagaaagg tgacccgccc gaggcggttg
     8701 tcctttaagt agcggatcgc gtcggacgcg atcccgtcgg tatcgacgat cacccagcgg
     8761 agccttccgg ccgccgccac gtcgagagcg gtcgcgtact ccggcggcgc ccggccaagc
     8821 tgcgccacgg tgccgtggac cccctccatg ccgaggatgg cgtccatcgc cttcccgccg
     8881 gcatcgccct gcgcctgctg ctgcgcctca aggcgcatca ggctctgctg gttctcccgg
     8941 atctcctttt gcagccggtc aagagccgag cgccgcgcaa agagcgtgct ctcggcctcg
     9001 gagaggtcgc gctcgatccg gcgcttctcg gtctcgcagg tggccatgga agaggcgtag
     9061 tccgccatct gcgcccgttt gttcaccagt tcctcgtcga tctggcgggt gcgcgcctcc
     9121 aggcgctcgc gctccgacgt ccgcatccgg ctcttctcga tcaggatatc ctgctcgcgg
     9181 aggatctttg cccggagctc ctttctgttc tcgaggtcct gcatccgcgc gaagagctgg
     9241 tccttgatcc cctcgacctc tttgctctcg ctcgcgatac gtttctcgac cgccgcaagc
     9301 tgatcgcgct ctatcgatgc ctccatcgcg aggtttgccc ggtcgatcga gagagtgcgg
     9361 atctggccgg cgcatccctc gaccttcgcc tcggcgcgcc tgccgtccat gtagacccgc
     9421 tggatggtct cgaggttctc gtcccggccg gccgcgaggc gctcgatggt cttctcggcg
     9481 agtttgatcc cgccccgtgc ctcctcgagc cgcccgacga gcgcgaggta ttcgggaccg
     9541 ctctttgtgc tgatctcctc ttcgaccgcc tgccggcgct cgcgcgcctc ttcagcctgt
     9601 gcccggacgg cgtcggcctc gccggcggtg cgcccgagcg cctccttctg gtcgagcgtg
     9661 agcgcatgca gggtaccgat ctcctgctcc ttctgccgga cgagcgcagc cccccggcag
     9721 cggccgaggt gctcgagttc ctcctgccac cgccggtact ccatcgcctg ctcgcgctcg
     9781 tgctggagcg catccagtcg tgccacgagt tcggagagga ggatctcctc gcgctctatc
     9841 cgctcccgga ccacctcgag ctccgagaac gcctgctcgc gcttatggtc gaactcggcg
     9901 acacccgcta tctcgtcgac gatctttcgc cgctctccat cgctcatctc catgatgcgg
     9961 gtgatatcgc cctgcatcac gacgttgtag ccttccggtt tgatcccgat cttcgcgagg
    10021 aattcgatga cctcgctctg cttgcagagg cggttgttta agtagttgta actatagtac
    10081 cccgccgggg tccgcttgat cctgcggcga atcgccgtgc cgtccgagaa cgtcgccgtc
    10141 acctcggcgg tgttcttccc ggtgttgacg ttgatgagat cggtcagttt ctcggcccgc
    10201 agcccccgcg cacccgagag agccaggaca aagaggatgc tgtcgatgat gttgctcttc
    10261 cccgagccgt tcgggcctga gatgaccgta aaccctccaa aaaaagggat tttcgtcttt
    10321 ttcgcgaagg acttgaagtt gtcgatctca agctgcgtga tatacaagga tcgggctccc
    10381 ctgtctacct gtcgagatcg accgtgtcgt cctcttcagc ctggatgacg ttctcgacct
    10441 cccgcttctt ctgctccacg aagagggtcc ggtcggatga cttgccgccc cggctccggc
    10501 tctggccttt catgggagcg ttcccgaact tgttgctggc aacgatgtac tccgaggatc
    10561 tccgcgattc cggtttcagc gtcccatcgt tctgcatgat gagttccatg tccgcatcat
    10621 cctggtttgc ccggaccggc tgcttctccg ccggctgcgc gggctcccgg ggcacggccg
    10681 caacacgggg ttccacggcg gcgcgaggtt ctgcctggag cgtcgccccg gccttcttgc
    10741 tctcggccgt aaccgactta accctgcgct cctccgcttc ccgggagagt ttcatcgtca
    10801 cggacttgag atccaggatc tcttccgtca ggcccctgac cagggcctcg agctcccgca
    10861 ccttgcgctc cagatcgcgc aggcgctcat cgttcacggc cggtgccgcc ggctgttcgg
    10921 gttctgtcat cgtctccatc tccttctcgc gttccgcaag ttccctctca aggaaccgta
    10981 tcctcgcatc tttctgtgcc attgtctccc tcgaattaat ccttgataat aattacgtaa
    11041 taatattact atggatttcc gaaaagaaga atgattacat tttatccggg atctgccgga
    11101 caccctgccg gaagtcactg gataggcagt cgacgcggac aaattctgca aaagaaagaa
    11161 tattacatcc ccagagcaac cttttcctgc atgtcttatc tggcaaactt cgatccggaa
    11221 gtcgcaggca tcatcgagaa cgagcgcctg cgccaaatca acgggttgga gctgattgcc
    11281 tcagagaacg tcgtcagcaa ggcggttctc gaggcgaccg gctccatcat gaccaataag
    11341 tacgccgagg gctaccccgg caaacgttac tacggcggtt gcgagtacca cgacgccgtg
    11401 gagaacctgg cgcgcgaccg gctctgcaaa ctcttcggcg cggagcacgc aaacgtccag
    11461 ccgcactcgg gaagccaggc aaaccaggcc gtctacttcg cctacctcaa ctacaaggac
    11521 aggatcatga gccagagcct cacccagggc ggccacctct cccacggctc gccggtcaac
    11581 atcaccgggc gctggtactc catcttccac tacggcgtcg actccgtgac ggagacgctc
    11641 gactatgcgg tcatcgagga tatggcgcgg attgcaaagc cccagatcat cgtctgcggc
    11701 gcaagtgcct acccgcgcga gatcgacttc aaagcgttcc aggagatcgc tgacaccgtc
    11761 ggtgcgcggt gcatggccga tatcgcccac atcgccggac tctgcgcaac cggataccac
    11821 aactccccgg tcggcgtcgt cgatatcgtg acgacgacga cgcacaagac cctgcggggc
    11881 ccccggggcg gcgccatcat gtgcggcaaa gagaacgccg ccgccatcga caagtccatc
    11941 ttccccggca tgcagggcgg tccgctgatg cacatcatag ccgcaaaggc ggtctgtttc
    12001 aaggaagcgc tgacccccgc atacggggac tactgcgggc aggtcgtcag gaacgcacgc
    12061 acgatggccg atgtcctcac cgaggaaggg ctcgatctcg tctccggcgg caccgacaac
    12121 cacctgatcc tcctcgacct gaccggggtc tccacgaacc acgagcacct caccggcctt
    12181 gcggccgaga ccgctcttgg cgaggccggc atcaccgtga acaagaacac catcccccgc
    12241 gagcagctca gccccttcgt gacgagcggt ctacggatcg gcacgccggc cgtcacctcc
    12301 cgcggcatga aagaagagga gatgaaacag atcggccact ggatcgcccg ggtcgtgaag
    12361 gacgtcgccc gggaccggac gtcaaagaag gcaatcaccg aggtgaggga agaggttatt
    12421 gccctcgcaa gcaagtatcc tctttatcct gaagtagcat gatactcgat ggaaaagcgg
    12481 tctctgagaa gaggctcgag atcctcaagg agaagataga cgagtccggg ctctacccgc
    12541 gccttgcgac cgtcatcgtc ggagaggacc cggcatcgca gatgtatgtc cggatgaagc
    12601 accgggcgtg cgaacgcgtc gggatcgggt ccatcgggat cgagctttcg gcagatgcct
    12661 ccacggagca ggtgctcgag gcggtcgcgc gcctcaacac cgacccggat atcgacggca
    12721 tcctggtcca gctgccgctc ccccggggcg tggagaccac ccgcgtcatc gatgcggtcg
    12781 cgcccataaa ggatgtggac gggttccacc ccttcaacct gggccgcctc cttgccggga
    12841 aaccggtcta cgccccctgc accccgcagg gggtcatgac gatcctcgat gagtacggta
    12901 tcccgatcgc cgggaagcgg gcggtcgtcg tcggccggag catcgatgtg gggaggccca
    12961 tggccgtcct gctcttgaat gccgatgcga ccgtcacgat atgccactcc aggaccgaaa
    13021 acctcgccga tgagacgcgg agagccgata tcctggtcag cgcggtcgga aaggcgaagt
    13081 ttgtcaggcc ggatatggta aaggccggcg cgacggtcat cgatgtcggg atcaaccagg
    13141 acgagcaggg cagactctcc ggcgacgtcg acttcgaggc ggtgcgcgag cgggcggggg
    13201 cgataacccc ggtccccggc ggcgtcggcc ccatgacgat cgcgacgctg atggagaaca
    13261 ccttcaaggc tgccaggttg aggacatgct gcgacgagat tgtacgataa accgcctccg
    13321 gatcggcggc gacgccccgg ttcgcctgat gggcgtcatc aactgcagcc ccgagtcgtt
    13381 ctaccggggt tcctacaccc cggccggccg ggtgtacgac cgggctctcg ccatgatcga
    13441 tgcgggcgcc gacctgatcg acctcggggc gcggagcacg gccccgggtt ctcccccgat
    13501 caccgtcgcc gaggaggcgg cgcgggtgga ggcggcgctc tcggagctcg acgggaccgg
    13561 tatcgccgta tcggtggata cccgctatgc ggaggtgctc gatctcgctc tccgccacga
    13621 tatccacgcg gtcaacgaca tatccgggct tgccgacgag cgctacgccc gggcggtcgc
    13681 cgactccggt ctcccggtct ttgtcatggc aagcctccgg gagcccggcg atgcggtcgg
    13741 tcttgccgca accctcgccg cgctcgatgc ggtcgtggcg cggtgcacgc gcctcgggat
    13801 cgaggagtac gtcctcgacc ccgctgtcgg aagatgggtc ccggggagga cgagcgagga
    13861 cgactggggg ctctgccgga acttcgaggc gttctcatcc ttcggccgcc cggtgctcgc
    13921 cgcggtctcg cgaaagacgt tcatcgggga cctgctcggg agggagcccg acgatcggct
    13981 tgccggaacg ctcgcgctca cggcgatgct ggtggagaag ggggcggccg tcgtgcggag
    14041 ccacgacgtc cgcgagaccg ccgacctcct gcgggtgtcc gcgaagatga ggggacgatg
    14101 acggcgccat cgttctcggt ctacgggctt gcgaccgcgc tgatacgtgc cggcgacgac
    14161 gtcgcggccc acctcatcga ggcggccgct cgtgcggggt gcgaagggtt tcagaccggc
    14221 gatgtcgtgg tcatcgccga gtcggccgtg gccaccgccg aggggcgggc cgtgcagctc
    14281 gccggcatcg agccgtccga cgaagcgcgc aggctcgccg aaaattaccg tatggacccc
    14341 tgccaggtcg aggtggtcct ccgcgagagc gaccgggtcg tcgggggcat accggggttc
    14401 ctgctctgca tgaagaacgg gacgctcctc ccgaacgccg ggatagacgc ctcgaacact
    14461 cctcgcggtt ccgtcctcct cctgccggcc gaccccgacg catcggcggc gcggatacgc
    14521 gcgacgatcc gggagcggtc gggggcggac gtcggcgtca tcgtcatcga ctcgaggacg
    14581 cacgcgatgc gccttggatg cagcggggtc gccatcgggt gttcgggtat accgtcggtg
    14641 ctcgacgagc gcgggaagca ggacctcttc ggccgcgagc ttgaggtcac caaacgggcg
    14701 gtggcggact gtatcgcgtc cgccgcagaa ctcgtgatgg gggaggcaaa cgagtgcgtc
    14761 cccgcagccg tggtgcgggg gcttatgctc ccggtcggcg actataccgg ggtcgccacc
    14821 atcgacgcgt cagagtgtct ctttatgggc gtcgcgctgc acgccgaccc gtcccttctc
    14881 gtcgatgaca agagagaacc ctgagttctc caggtactcc cggctcttcc tcccttttcc
    14941 atggacaagg atctcgacca ggtcctcctt ctcgtcgatg tcggtcgaca tccggaacga
    15001 gtcgatcacc tcgacggaga aaccgcactc ccctgcgatc cgcatgtggt cgaggaagct
    15061 tgcgccgtag tagtcggcgc ggaaacaccg gggtttcttt aagaatatga tgttcgtccc
    15121 gccgccccgg cccggcacga tggccatgtc cttctcggtg cggatcagcc gctggatatc
    15181 cccggccgtc accaggggga tgtcggccat gatgatgagc gccggacagt gaaactgacc
    15241 gagcgcccag ttgatcgcgt cgttcaacga ctctttcctg acggcgatga gcgcgttctc
    15301 gtgcttgaac gggtgggtgc agaggagggt ggcgctgcac cccgacttca gcacggaggc
    15361 gatcacatct tcaagcatcg cccgggcgaa cgcctcccgc tcttcctggt cgaggatgca
    15421 ggagaggcgc gtcttggggt tgaccggctt aaaggggatg agcgcgtgga agtacatcgg
    15481 gaaaatcgtt gtcggggcag cctcaaaaag gcatcgctgg atgcgtaccg gagagccccc
    15541 cacccgcctg ctttatgtcc tctgcgtgcc ggtgcggcga agcgccgggt gccgcgcttc
    15601 agtgtgaata gttgtaagaa taccggaact ctaacagagc gccggtattc gcattaactt
    15661 tcattccagc ccgtctcggc cattcgaaat cttcaatctt ttcaatccag gagagattca
    15721 gcctccaggc gccgctttct aaaacagcca acgtgatcct gaactcttca acatcctggg
    15781 ctgttttcag atatttcgcc actgtgttgc gcagttcgtc aaaaggcaaa ttctctccgg
    15841 gtatactgac cgccccccaa tgatgataac cggattccgg gacaaaaaag cgttttcctc
    15901 atgccggata tcccggcgtg cagaggggtg cgccgcctcg cgcagatcgc ccgcgagaga
    15961 cggcatcgcc atccctacct tcaagtggag ggatgccgac atatacccat gcaccgccgc
    16021 gtgattacct tctcacgaaa cgcgttcctc cccttaacga cggtctgcag gaaccgttgc
    16081 ggctactgct gtttccggac gccggtacgg gagggatgcg tcatgcctcc cgacgatgcg
    16141 ctccgcacca tggaggcgag cgcacgcctc ggctgcacgg aggcgctctt caccttcggg
    16201 gagcgcccgg gagcggtggc gggattcgac gccgtgctcg gcaggatagg gtatgccgac
    16261 atactcgact acgtctacga cctctccctt gcggccatag aacgggggct cctcccgcac
    16321 accaacgccg gcatcctctc ctacgacgaa ctcgaccggc tccgcggcgt gaacgcgagc
    16381 atggggctga tgctcgagac gaccgcggat atccccgccc accgtaactc ccccggaaaa
    16441 gacccggcgg tccggatcga catgatcgag aacgccggca ggctctcgat accgttcacg
    16501 accgggatcc tggtcgggat cggcgagacg atggaggacc gcgaggagtc gctctcggtc
    16561 atccgggacc ttcacgtgcg gttcggccac atccaggagg tgatcgtgca gaacttctgc
    16621 ccgaaacccg ggacgctgat ggagggtgcg ccggcgcccg gcaccgacga gctctgcgcg
    16681 acgatcgccc ttgcccgcga agtcctcccg gcagacgtcg cggtgcagat acccccgaac
    16741 ctcgcggatg catcgcacct ggtcgggtgc ggcgtcgacg acctcggcgg ggtctccccg
    16801 ctcaccatcg actacgtcaa ccccgagcgc ccctggccgc agcttgaaga actgaggaga
    16861 gtggcgggag acgccgagct gcgggaacgg ctctgcatct acccgcagta tatcgagaaa
    16921 ggctggtatc caccgcttct cgaacccctg atccggcgtc tcgcggcacg gatcgcggca
    16981 cccggcggag gtgagggagc atagtctcat catacatccc gatcaacacc tgggaggaga
    17041 tctggcatga aacaggttga cctcctctac tcagggaagg cgaagtccgt ctatcgcacc
    17101 gacgacccgg gcgtctacat catgaagttt cgcgacgaca tcacggcctt cgacggcggg
    17161 aagaaagata ctctgggcgg caaagggaga tataacgcag aagtctcctc gttcttcttc
    17221 aggtacctgg aggagaacgg gataaagaca cactacctct caagcgtcga ccccaccgcc
    17281 atagcggtgc ggagcctcac gatgatcccg ctcgaggtga tcgtgaggaa cgttgcggcc
    17341 ggttccatcg tgcgcaacta tccgttcagg gagggagagg tgctcgatcc ggcagtgatc
    17401 gtcatcgatt acaagagcga cgcgcaccac gacccgatgc tcaacgacga cctgatctat
    17461 gccctcgggc ttgccacgcc cgaggaactc gaccagatca aggcgatggc gctcgccgta
    17521 aacatagtgc tctcggagta cctcgccgcg cgcggcatca ccctggtcga cttcaaactc
    17581 gagttcggca agaccgacgg cgagatcgtc gtcggcgacg agatcagcat ggactcgatg
    17641 cggctctggg ataaagagac cggggcatcc cttgacaagg atgtctaccg cttcaacaag
    17701 ggggacgtca tggagaccta cgccggcgtc gcgaaacgga tactctcgcc gcccggagaa
    17761 gagtccgcgt gagccatacc gcgaccgtcg ccgttgcacc ccggggagag atgcccggca
    17821 ccggggtgcg atccctctct ccggggagga aaaccggcat gccgccctga accgtgagag
    17881 aggggtcgcg ctccgtccct ctgatgggca cggcaacgcg acggcgggga gagcctgaac
    17941 gggccggcct tctctccgga gcaggaacca ccctcgcaac gatgccacgc cccgggcatg
    18001 cccgcagagg gcagggtcaa aatctttaac ttgatgacaa cccatatcga agagtacgga
    18061 cggcaggagg cctgattaag agta
//
LOCUS       DAOSII010000027        17993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_3342_length_17993_cov_48.512710, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000027 DAOSII010000000
VERSION     DAOSII010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 17993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17993
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3342_length_17993_cov_48.512710"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PLV90_03465"
     CDS             complement(<1..537)
                     /locus_tag="PLV90_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOF96201.1"
                     /translation="MAFDRVLNVAKKEFSDHITSRRFIIILALFLVISTVSIHEGIEL
                     YNTRLEMYNEQLRQMTAGFEGLYPGQMPEKPSIMLVFLFMMGYMTALGSILALAVGFD
                     LVSKEKESRSLKSLLSHPVYRDEIINGKMLGGVGALGFAMALALALSLAMLLVFSVVP
                     TLDEFAAILIFGAASLGLL"
     gene            complement(541..1446)
                     /locus_tag="PLV90_03470"
     CDS             complement(541..1446)
                     /locus_tag="PLV90_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF96202.1"
                     /translation="MIRTENLAKVYNGKAAVDGLDLEVGEGEIFGFLGPNGAGKSTTI
                     LMLTGMIEPTGGRCLVDGIEVAKDPLKVKKIIGYLPEDVGFYGNMTGEQNLDYFARFY
                     GMNAKERKERIAELLDLVHLGGVTQKAGEYSRGMKQRLGLAQALINDPRVLFLDEPTA
                     NLDPEGVREYRDLVERLAGEGKTVFVSSHILSEVRAVCRTVGLLAKGKLVAQGTLDEV
                     ARTLAPSDGDAVRIVVETQEHLPDIEDTELLAVERNGNRAVFRAKTDIRDRLAASLLE
                     QGIHVREMRIEEPDIEDIFMAAYRR"
     gene            complement(1450..2085)
                     /locus_tag="PLV90_03475"
     CDS             complement(1450..2085)
                     /locus_tag="PLV90_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1673 family protein"
                     /protein_id="HOF96203.1"
                     /translation="MMSLVETIRAYLGWCPMQGAMRADLPMRPDMTAAPGGQDGFPRA
                     GSGWWSRYHNQALVLAVAFSAAAAAAFLLIEDAPGYPTVWTGLAIGVGTVIGFLLTYR
                     RQYARVAAGEFIEANMTGRQRIVRRLSVPAAAVILVTLMAYFFLRGMFGWIVRFMLAL
                     SVIAWALYGVTILWERENRKILIMEKGSMYALGAEYGDEVPPGDMQADGQE"
     gene            complement(2082..2528)
                     /locus_tag="PLV90_03480"
     CDS             complement(2082..2528)
                     /locus_tag="PLV90_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96204.1"
                     /translation="MSLPLGDRQFTFWRLRRTGLPNVGIADRFGISRQAISKALLAMG
                     RKVEETLPEVAHANQIDVERLNAGKGVLFGHPVPFDAAAIVFVSANHGVQVRYEHEGD
                     CGACPRYARCIELLRDYADEPGIALKKTADPTRMADELFAKLREIV"
     gene            3486..3845
                     /locus_tag="PLV90_03485"
     CDS             3486..3845
                     /locus_tag="PLV90_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96205.1"
                     /translation="MQRRVVVEKAADAALAVVIFLIYLYASLAYSINQVFWLPVLVII
                     GLLLARIVFTDGGERRVTFARSAVFYLIAAGIFLLRYAVLEYPVLPLFQGIALVGIIT
                     FPILYIWERRRTPEGTD"
     gene            complement(4369..5010)
                     /locus_tag="PLV90_03490"
     CDS             complement(4369..5010)
                     /locus_tag="PLV90_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96206.1"
                     /translation="MDSAITFQCAFEGSLDGAVVRRLLRHAGALPGDLYRQRKSYLLE
                     KLQGFNVSARARPWVVVVDLDHDAGCAPEVVRDWLPAPSYFMNFRVAVREVEAWILAD
                     RERLARYLQVPETRITGTPEEIDYPKEYLINCARESSSSRIRKGIVPGPGSRRVEGPA
                     YLSLLTEFVADAERGWRPDVASDHSESLERCIRSLQNSIGTFSRESQRRRSSR"
     gene            complement(5012..6154)
                     /locus_tag="PLV90_03495"
     CDS             complement(5012..6154)
                     /locus_tag="PLV90_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOF96207.1"
                     /translation="MPKRFTHISLWNWRNFQRVDMDLQARVFLVGPNASGKSNFLDVF
                     KFLRDIVSVGGGFQGAVKERGGVSSLRALSARKYSDIGIMVRLGNAERDDLWEYELTF
                     RQDERNLPVIAKERVARCGNEVIARPSAQDEEDPERLTQTYLEQVNVNKDFREVADFL
                     KTTRYLHLVPQLVREPDRSVGYRNDPYGSDFLKQVMGRSKEERDSMLRRIERALSVAV
                     PQLSEIKVEFDSIGKPHLYGRYKHWKPQEAWQTEQQLSDGTLRLLGILWALLDDGGPL
                     LLEEPELSLHPAIIRYVPQIFSRVQLKSDRQILISTHSSELLQDEGIGLDEVLLLRPS
                     EEGTTVVSAGNIEEIDDLLKGGLSLADVVIPKTCPGDTAELAWFGE"
     gene            6370..6675
                     /locus_tag="PLV90_03500"
     CDS             6370..6675
                     /locus_tag="PLV90_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96208.1"
                     /translation="MRILKITVEEHPDAYVACPPGLKGVVIAEGDTHEEALAHSNPDS
                     TTMPNHPQIKGLTLRSICTQAGIPREEFLRAYEQEQAIQFPAFSPARAPYYPAPQTC"
     gene            6678..7031
                     /locus_tag="PLV90_03505"
     CDS             6678..7031
                     /locus_tag="PLV90_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase family protein"
                     /protein_id="HOF96209.1"
                     /translation="MITDRERAAFEAGIKLGALYHQFVGTPIARETAAGVEAAIEQAV
                     GLQPYVTEIRVRLNRDMMVSNRFGYSELSGRMFDAEITTTVGATVCRARLHLEDDYPM
                     MEIVDIYETSRDTDN"
     gene            7003..7833
                     /locus_tag="PLV90_03510"
     CDS             7003..7833
                     /locus_tag="PLV90_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOF96210.1"
                     /translation="MKPPAIPITDDHIHIDPVNGRGVEAAKDFRRSGGTHIFLVSKPS
                     WSFGVEPSSGEDYREVFLATLRVADMVRETGLVVYPVLGVHPAEINRLAARMSLEEAA
                     GVMMAGLDLAARYVEEGEAVALKSGRPHYEVAPEVLAASNAVLYHALELGADYDCAVQ
                     LHAESGPCTDVVDMAGRAGIPVERVVKHFATPDTPLMPSLIARHEDIPALARAGRHFT
                     MESDYMDENARPGAVIGPKSVPRFTHRYLKEGLITEEDAWRIHAATPSRTYGVDITLP
                     "
     gene            7878..8171
                     /locus_tag="PLV90_03515"
     CDS             7878..8171
                     /locus_tag="PLV90_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF424 domain-containing protein"
                     /protein_id="HOF96211.1"
                     /translation="MYLKVHHVPDAGEVVAACDSELLNVTLMHGDVEVCITGGFYGTE
                     RVAEEEVRRALLGATNANIIGKRVVALAVALGLVRREDCIMIGDVPHAQIFGM"
     gene            8175..9218
                     /locus_tag="PLV90_03520"
     CDS             8175..9218
                     /locus_tag="PLV90_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_function: GO:0043023 - ribosomal large subunit binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="60S ribosomal export protein NMD3"
                     /protein_id="HOF96212.1"
                     /translation="MAIQTSICPRCGQPTEEGLCPQCRAADTRLLACEPRVTAVYCPV
                     CDSQRRGKTWSDLRMPREDLIAEMAIAATSIHEDAKDIRVAVRSAETGPNRTTCTVDV
                     EGTLYSVPVHGTCEVEILWQKEACDRCSRISGGYYEGIVQVRATDRKINGYEREVAAS
                     IADQAEESLQQAGDRLSFISDLKDSKDGVDITVGSQHLGQEIARMITGALGGRYTTHP
                     KLVGEKDGKALYRVTYSIRLPFYQKGDVVVSHGGYFEVRDIDGRRLRAFDLATGATRT
                     LAEDEVERLIGNVREAESALVVFTQPDIVGLMDPKTYRTREQSPVPWTFPVEGQPIRV
                     LRDEEQDRLVIVG"
     gene            9228..10097
                     /locus_tag="PLV90_03525"
     CDS             9228..10097
                     /locus_tag="PLV90_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOF96213.1"
                     /translation="MRARRVPAAVLADIVGAEWVDPARRPYVRGGTAWVPVREGYPAD
                     ADLPERESYRGRGYHRIGDVAVLRGDAPSEAELAAIVEHCRPRGVLLVKGYLGDMRIP
                     DVEILYGNTGEVCHREQGYTFILDPARVMFAQGNRNEKARIASLVRPGERVADMFAGI
                     GYFTIPAAAGGAQVHAMEINPIAFEYLQRNSMANHVADRVTAENGDSRILLSGTYDRI
                     LMGHFDAPSMLADALAHAHSGSVLHVHSIGDVRGAIRDATAEAGFTAMVTSRRVKKYG
                     PHAWHMVQDVTIS"
     gene            10094..11224
                     /gene="coaBC"
                     /locus_tag="PLV90_03530"
     CDS             10094..11224
                     /gene="coaBC"
                     /locus_tag="PLV90_03530"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOF96214.1"
                     /translation="MKTFSGKTVVLAVTGSIAAVETVKLAHALRRRGAEVQAVMTEAA
                     TGIIHPDALTYATGRPAITRITGLVEHILYCGEGGAADLLLIAPCTANTIGKIAAGID
                     DTPATTFATTALGRRMPVVVAPAMHESMYRHPGVVENLERLRSWGIDIVAPRIEEGKA
                     KVADIDTIVLHAARAVSGRPLAGRRVLITSGACAEPFDDVRVLTTRSTGRMGRALALE
                     AFRLGADVTVVHAGSFPCVRNIRVATAADMRRAVLSVCRDGGVDIYMSAAAISDFAPE
                     RCEGKIPSGSPLDIRLNPLPKIIDEVMATCCPVTVAFKLGWHEEERARTMLDAGVKMV
                     VVNAPPAMGAAGGTFSLMMADSTIEVTGSKEEVAAAIWSGLL"
     gene            11206..12048
                     /locus_tag="PLV90_03535"
     CDS             11206..12048
                     /locus_tag="PLV90_03535"
                     /EC_number="2.7.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate kinase"
                     /protein_id="HOF96215.1"
                     /translation="MVRTAVAFSPGHISGYFRRVEGSDPRTTGSVGAGVVIDEGVRAT
                     VVPAAKTGVRVIRQGRVSTGSPPVEYALDRLGVTARVTTECRLPIGAGFGLSAAALLA
                     TLTAVNHLFDLGLSADEVASRAHEAEVVHRTGLGDVAASRSGGVVCRMGPGIHADIAR
                     FYPDEPLYAVSLGPLPTASVLSSEEAMARIASAYPDRCPRDIADFCFLSRQFAERSGL
                     IAPDVRKVLSACDAAKIPASMTMLGNGVFAAGNSAERVLSGFGEVYRLNVARRGAYLI
                     EMRP"
     gene            12045..12776
                     /locus_tag="PLV90_03540"
     CDS             12045..12776
                     /locus_tag="PLV90_03540"
                     /EC_number="6.3.2.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphopantoate--beta-alanine ligase"
                     /protein_id="HOF96216.1"
                     /translation="MIPKDHPRYRSLIARERLARCAREGIVALEGLAAHGRGEAFDYL
                     IGERTSESAALAARTAAAMLLSARHPVISVNGNTAALAADEIAALQKASGADVEVNLF
                     HRTEERIRLITRLLEEHGVRVLSGTAERLVPLAHDRALSLREGIGDADVVLVPLEDGD
                     RCAALRAMGKTVITIDLNPLSRTARTATLTIVDELTRAVPAITVACASLSPGEGERLI
                     ASLDNIYLLRAAIDEMRERLSHALE"
     gene            12763..13773
                     /locus_tag="PLV90_03545"
     CDS             12763..13773
                     /locus_tag="PLV90_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOF96217.1"
                     /translation="MLWSEVYRPKRCEDIIGQDAVIRYLVSFADAGSVPHMILSGPHG
                     TGKTVAVECLAKRLYGENWEANTTVLSAADLLGRGRSALETDERFAQIYRKDQSLIVN
                     FKYVVKWYASMRPLDADFKLMVFEDAEGLTFEAQQALRRTMERYSATCRFIFVTTRPS
                     AIIPAIASRCLPLFFAPLESALIRTRLAEILAAEAATCPADDIDLIVHAARGDLRRAI
                     TYLQIVAGAGEEIDLAEVARSETSNVATSAFEALRAGNFAAARSIAESLMIEYGLPAR
                     EVVGELRRAVRREYNHPALAIALADADCQLGHNANDFVQINALLARIAREVSSEESTA
                     AL"
     gene            13748..14464
                     /locus_tag="PLV90_03550"
     CDS             13748..14464
                     /locus_tag="PLV90_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOF96218.1"
                     /translation="MKKVQQHYDEIADIYDDRYDGNRGKYYHGHISDHVMGVLPRGGF
                     LLDIGCGTGLFVQRYVEEGGRAVGLDISPGMVRHGRQRCPASGFFVGTADVLPFKDAT
                     FDALASLLAFSYVPDPEAMLRECYRILKPGGRIAVCTLSRTVFTSIVPIAYQVGEKVG
                     LKKVGVGDFGEHYYTNSEIARLFCEAGFEDVAVSRCSFAHLNLADPVFYLARKVEPFI
                     EENLPYLAYNVCAGGKKPEG"
     gene            14593..15138
                     /locus_tag="PLV90_03555"
     CDS             14593..15138
                     /locus_tag="PLV90_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOF96219.1"
                     /translation="MEPVDIVLTKDTFEVLASETRLTILKTLNERRMTITELADNLGL
                     AKSTIHHHLQKLTGAGFVAADGDGHAWVYYALTPEGRALLQPHGSARIRIILTAGLAS
                     LAGGVCALAAFLTAPVREGPVFPGPKGGGGLPVPEGAPVELLVAGVALAAVGGALMTY
                     ACVRWRNTRAAGYFPADEKSA"
     gene            15295..16587
                     /locus_tag="PLV90_03560"
     CDS             15295..16587
                     /locus_tag="PLV90_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOF96220.1"
                     /translation="MKGTIWLFSGISILILALILVGLTAAGTAPQNTTSISGKPVAGD
                     AGIAHPDTPDGAGNVAAGNNRFAFDFYRRLATDPAHAGDNLFFSPYSISSALAITCEG
                     ARGATADEIGAVLHLPTNETLRREGFAAIDTGLNCASGNYTLRTANALWAEKTHSFLP
                     EYIDVAVRWYGANVTNLDFVENPGGSRETINRWVEEKTEEKIRDLLPPGSIDSMTRIV
                     ITNAIYFKGTWAEQFDPDGTTGEEFRVGPNETVTVPMMHGKTIYPYAETDTLRVLEMP
                     YAHGNETELAMLVLLPKEDNLTAAEEALDTERLAGLQESLVSQNVRVFFPKFTLETGY
                     PLAGVLAAMGMPTAFSPDADLSGMDGTRNLFITGIYHKAFVDVNEEGTEAAAATGVIG
                     GKGVTPVFRADHPFVFLIVEKNSGTILFAGRVVNPESP"
     gene            complement(16652..16819)
                     /locus_tag="PLV90_03565"
     CDS             complement(16652..16819)
                     /locus_tag="PLV90_03565"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOF96221.1"
                     /translation="MIGLRNVLVHAYFRVDPEEVWAVVNRDIPALREEVAMLLSLLGG
                     TDGAGQPTIPS"
     gene            complement(16816..17022)
                     /locus_tag="PLV90_03570"
     CDS             complement(16816..17022)
                     /locus_tag="PLV90_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96222.1"
                     /translation="MRDGIDRLLDIQEAIKEIERYAVRGRDAFLQDELVQVWMVHHLR
                     IIGEAAARLPLTLEALLPAFPGNR"
     gene            complement(17019..17312)
                     /locus_tag="PLV90_03575"
     CDS             complement(17019..17312)
                     /locus_tag="PLV90_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOF96223.1"
                     /translation="MPVTVDLIREKRSRILAIAGRHGARNLRVFGSVARGEAGPESDL
                     DLLVELEPGRSLLDHIALIQDLEEALGCRVDVVTEAALKERYKKRILKEAVPI"
     gene            complement(17524..>17993)
                     /locus_tag="PLV90_03580"
     CDS             complement(17524..>17993)
                     /locus_tag="PLV90_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOF96224.1"
                     /translation="TDVTNGGLRGDAYEMAETAGCRIVVEEDELRTLVQPKVLAMLDA
                     LEIDYLGVSLDALLVVAPPEVAPDIRRVVASAGVAMKEVGYVEKGAPESVLSVGGEIR
                     DFSPRFRESAYTPVKKVVDEDKRDFAEMKAGVERAAEAALAKKLRILSRLRSS"
BASE COUNT         3329 a         5741 c         5575 g         3348 t
ORIGIN      
        1 caggagcccg agcgaggccg ccccgaagat caggatggcg gcgaactcat ccagcgtcgg
       61 gacgaccgag aagacgagga gcatcgcaag tgagagggca agcgcgagcg ccatggcgaa
      121 cccgagcgcg ccgacgccac cgagcatctt gccgttgatg atctcgtcgc ggtagaccgg
      181 gtgggagagg agcgacttga gcgaccggga ctccttctcc tttgagacca ggtcgaaccc
      241 gacggcaaga gcaaggatac taccgagggc cgtcatgtaa cccatcatga acaggaagac
      301 gagcatgatc gagggtttct cgggcatctg gcccgggtaa aggccctcga aacccgccgt
      361 catctgccgg agctgctcgt tatacatctc caggcgggtg ttatacagtt cgatgccttc
      421 gtggatgctg accgtggata ttaccaggaa cagggccagg atgatgatga accgcctgct
      481 ggtgatgtgg tcggagaact ctttctttgc tacgttgagc actcgatcga acgccatgct
      541 ttacctccga taggccgcca tgaagatgtc ctcgatatcc ggctcctcga tccgcatctc
      601 ccggacgtga atcccctgtt caaggaggga ggctgcaagc cggtcccgga tgtcggtttt
      661 cgcccggaag accgcccggt tcccgtttcg ctcgacggcg aggagctcgg tatcttcgat
      721 gtccggcagg tgctcttggg tctcgacgac gatcctgacg gcgtcgccgt ccgaaggtgc
      781 gagcgtccgg gcgacctcgt caagcgtccc ctgcgcgacg agttttcctt tcgcgaggag
      841 cccgacggtt cggcagaccg cacggacctc ggagaggatg tgggaggaga cgaagaccgt
      901 ctttccctcg ccggcgaggc gttcgacgag atcccggtac tcccggacgc cttcggggtc
      961 gaggttcgcc gtcggttcgt cgaggaagag tactctgggg tcgttgatca gcgcctgggc
     1021 gaggccgagc cgctgcttca tcccgcgaga gtactccccg gccttctggg tgacgccgcc
     1081 gaggtggacc aggtcgagaa gttcggcgat ccgctccttg cgttccttcg cgttcatccc
     1141 gtagaaccgg gcaaagtagt ccaggttctg ttcgccggtc atgttcccgt aaaacccgac
     1201 gtcttcgggg aggtagccga tgatcttctt tactttcagc gggtctttcg cgacctcgat
     1261 cccgtcgacc aggcaccgcc cgccggtggg ctcgatcatg ccggtgagca tcaggatcgt
     1321 cgtgctcttc cccgccccgt tcgggccgag gaagccgaag atctcgccct ccccgacctc
     1381 caggtccagg ccgtcgacgg ccgcttttcc gttgtatacc ttcgcgaggt tctccgttcg
     1441 tatcatgttc tactcctgtc catccgcctg catatcgccc gggggaactt catcgccata
     1501 ttccgcaccc agagcataca tcgacccttt ctccatgatc aggatcttac ggttctcccg
     1561 ctcccagagg atcgtaacgc cgtacagggc ccaggctatg acgctcaggg ccagcatgaa
     1621 ccggacaatc cagccgaaca tacctctcag gaagaaatac gccataaggg tgacgagaat
     1681 aacggcggcc gccggtacgc tcaggcgccg gacgatgcgc tgccttccgg tcatgttggc
     1741 ctcgatgaac tcaccggcgg cgacccgcgc gtactgcctc cggtaggtga gcaggaagcc
     1801 gatgaccgtt cccaccccga tggcaagacc cgtccatacg gtcgggtacc ccggagcatc
     1861 ctcgatcagg agaaaggctg ccgctgccgc cgccgagaag gctaccgcta gaaccagcgc
     1921 ctggttgtgg tagcggctcc accacccgga cccggctcgg gggaacccgt cctgcccgcc
     1981 cggtgccgcg gtcatgtccg gccgcatcgg caggtccgcc cgcatcgccc cctgcatggg
     2041 gcaccagccc aggtaggctc gtatcgtctc cacgagagac atcatacgat ctcccggagt
     2101 ttcgcgaaga gttcgtcggc catacgggtc gggtcggcgg tctttttcaa cgcgatcccc
     2161 ggttcgtccg cgtaatcccg gaggagctcg atgcaccggg cgtaccgggg gcatgcgccg
     2221 cagtcgccct cgtgctcgta ccggacctgc accccgtggt ttgccgatac gaagacgata
     2281 gcggcggcat cgaaggggac gggatgcccg aagaggaccc ctttccccgc gttcaggcgt
     2341 tcaacgtcga tctggtttgc gtgcgccacc tctggaagcg tctcctcgac cttcctgccc
     2401 atggcaagca gcgccttcga gatcgcctgg cgggatatgc cgaagcggtc tgcgataccg
     2461 acgttcggga gaccggtgcg acggaggcgc cagaacgtaa attgcctgtc gcccagagga
     2521 agagacatat gtcaacactc attggttgac gctatatagt ttacggtttg ttcctctccg
     2581 aatccctctc gctggcctgt ccgggtgccg ggcggcccgg aagcgttccg gtgcagggca
     2641 accggcggcg ccccgcccgt cccccctcct gtcgcccggg gagtgcagcg gcaatcaccg
     2701 tgctcccatc tccggtgatc gcgacagggt gcacgccgcc ccggtgcgcg acggtccccc
     2761 gcgcctcgat gcggccgatg aactcttgtt tggggcggtc attcgcgcgg ggcttaaccg
     2821 gaagagtctc agtcaaagcg tggcggagcg gtgaaaggga gaaggatcta agtctcctat
     2881 gcagggtcac gttcgattgc tcgttcggac tcgtgaatat ccatgggatt atagtgattt
     2941 ggagatcgaa aagaatatga tgagggactt ctaaagggta aaccgtcggg cgacttgttc
     3001 agcgatgggt aagtcgcgga ttgcaggtaa aattgcggag gcccacccaa tccgtccaag
     3061 gtcgtgcgag cccccccact acccgaaggc gagatgcagt tttgcattcc acgtacttaa
     3121 gtactgcgcc tcttctgcct ccgggaatgc tgcaggagga aagcaaatga atggaacaat
     3181 cggaatgcgg gcgctctccc cgatggtgct ttggtgatag gtgcatgaaa ttcgacccta
     3241 tcgtcaagga ggcgctctgg cgttcagtcg tagtcttttt cgtgtcgatc atcatcgtcg
     3301 ctatcatcat cgggacagct ccccttccga tacctgcgtt cttgctcttt cccctggcat
     3361 tggcgatcgc tgcaatatat ttcatggtta tggtatggta tggacgaaag gcagccggac
     3421 gatcgagatc tcacgcacat ccgccttcat cccttccggt tagcggatac cgggaaggag
     3481 aaaagatgca gaggcgagtc gttgtcgaga aggcggccga tgccgccctt gccgtcgtga
     3541 tcttcctgat ctatctctat gcgtcccttg cctactccat caaccaggtc ttctggctcc
     3601 ccgtccttgt catcatcggc ctgcttctcg cgcggatcgt cttcaccgat ggcggtgagc
     3661 gccgcgtcac cttcgcgcga tcggctgtct tctacctcat agcagctggg attttccttc
     3721 tgcgctacgc ggtgctggaa taccccgttc tccccctctt ccagggaatt gcgcttgtcg
     3781 gtatcattac ttttcccatc ctctacatct gggaacgacg acgcacaccg gagggaacag
     3841 attgatggct gagcaagggg ctgcggagtg ggatgtctta tgctgagatc gttccactgg
     3901 cgtcaggtta attttgagag gggggtatct ctcatcgcac ttatggctcc tattccataa
     3961 agtagcactt tatatgtcca tcaggtcgct cattcccgtg accacaacga ctccaccctg
     4021 tcgagcgacc cactgtcacc ctctttcccg gacttacccg tcaatctctg gcatcgagca
     4081 caccaaggtc acgtgatccc cggttgatca cgccattttt tcaaaaaaac gtcaatttag
     4141 gcgtattttc cagttctttt ttcgaaagct cccgctctcc ctgacagacc gtgttcgagc
     4201 cggatgccct ccggtcgttc aggtcaacgc ttcaggatag cgtctcgggc cagcttcttt
     4261 gggtggtatt ggagaccgtg acggcagagt cgagcgaacc agaggttcca tccctctctg
     4321 ccccgggcaa aaacgaatga gcgaatacag agaagcatcg ggggcggttt accgggaaga
     4381 gcgccggcgt tgcgactcac gggaaaaagt gccgatagag ttctgcagag accggatgca
     4441 tctctccagg ctttctgagt gatccgacgc aacatccggt ctccatcccc gttcggcatc
     4501 ggcaacgaac tctgtgagga gcgagaggta cgcagggccc tccacccgcc ggctgcccgg
     4561 ccccgggacg atgcccttcc tgatcctcga ggaactcgat tcccgtgcac agttgatcag
     4621 gtattccttc gggtaatcga tctcttcggg cgttccggtg atgcgtgtct ccggaacctg
     4681 cagatacctt gccagtctct ccctatcggc caggatccag gcctcaacct ctctcacggc
     4741 cacgcggaag ttcatgaagt acgaaggggc gggcagccag tcccgaacaa cttccggcgc
     4801 gcacccggca tcgtgatcca gatcgacgac cactacccag ggccgggcac gggcagaaac
     4861 gttgaatccc tgtaacttct ccagcagata ggatttcctc tgcctgtaga gatcccccgg
     4921 cagggccccg gcgtgccgga gcagccgtct cacaactgct ccatccagcg atccctcaaa
     4981 agcgcactga aacgttatcg cactgtccat cctactcccc aaaccaggcc agctctgccg
     5041 tgtcgccggg acaggtcttc gggatgacga catctgcaag ggataggcca cctttgagca
     5101 ggtcgtcgat ctcctcgatg ttgccggccg acacaaccgt cgtaccctcc tcagagggcc
     5161 ggagcagcag cacctcatcg agcccgatgc cctcgtcctg cagcagttcg gaggaatggg
     5221 tacttatgag aatctggcgg tcactcttca attgcacccg ggagaagatc tgcgggacat
     5281 acctgattat cgcagggtgg agcgagagtt ccggctcctc gagaaggagc ggtcctccgt
     5341 catccagcag cgcccagaga atgccgagga gcctgagtgt tccatcagag agctgctgct
     5401 cggtttgcca ggcttcctgc ggcttccagt gtttatatct cccgtagagg tgcggttttc
     5461 caatgctgtc gaactcaacc tttatctcgc tgagttgtgg caccgcaaca ctgagcgccc
     5521 tctcgatccg gcgcagcatg gaatcccgct cttcttttga tctgcccatg acctgcttga
     5581 ggaaatcact gccatacgga tcgttcctgt aaccaacaga ccgatccggt tcgcggacca
     5641 gttgcgggac gaggtgcagg tagcgggtgg tcttcaggaa atcagcgacc tccctgaaat
     5701 ctttgttgac gttgacctgc tccaggtatg tctgggttaa cctttcggga tcctcctcat
     5761 cctgcgcgga gggacgggcg atcacttcgt tcccacacct tgcaacccgt tcctttgcga
     5821 taaccggcag gttacgctcg tcctgcctga acgtcagctc gtattcccag agatcgtcgc
     5881 gctcagcgtt acccagccgc accattatgc ctatatcgga atactttcgt gcagagagcg
     5941 ccctgaggct ggagacacca cctcgttctt tcaccgctcc ctgaaatccg ccacctacag
     6001 atacgatatc ccgtaagaat ttgaatacgt ccaggaagtt ggatttcccg gatgcgttgg
     6061 gtccgacaag gaaaacccga gcctgcaggt ccatatcgac cctctggaag ttgcgccagt
     6121 tccagagaga gatgtgggta aatctcttcg gcatatccac gcatctggag cagttaccca
     6181 tgatactgat cgattgaagc cccgtgtata ttaccctttt tgaggaccgg aggttccctc
     6241 acgcagtatg cccggagata agaggctgat ctgcaacctt cgccggcaga gaaaccaggc
     6301 taaatatatc gtcaggtagg ttgaatacca caccgatcgt cctgttccat tcgactttga
     6361 gagaaaccta tgaggatctt aaagattacc gtcgaggaac accctgacgc ttatgtcgcc
     6421 tgccccccgg gtctgaaggg tgtcgtcatt gcagaggggg acacccatga agaggcgctt
     6481 gcccacagta atccggacag cacaacgatg ccgaaccacc cacaaatcaa aggcctgact
     6541 ctgcggagca tctgcacaca ggcagggatt ccgcgcgaag agttcctgcg agcgtatgag
     6601 caggagcagg caatacagtt ccctgctttc tccccggcaa gagcccctta ttaccctgca
     6661 ccacaaacct gctaaccatg atcaccgacc gcgagagagc ggcgttcgaa gccggtatca
     6721 agctcggcgc cctctaccac cagttcgtcg ggacgcccat cgcccgcgag accgccgccg
     6781 gcgtcgaggc cgcaatcgaa caggccgtcg gccttcagcc ttacgtgacg gagatccgcg
     6841 tccggttgaa ccgcgacatg atggtctcga accggttcgg ctactcggaa ctttccgggc
     6901 ggatgtttga tgccgagatc acgacaacgg tcggcgcaac cgtctgccgc gcccggctcc
     6961 atctcgagga cgactacccc atgatggaga tcgtcgatat ctatgaaacc tcccgcgata
     7021 ccgataactg acgaccacat ccacatcgac cccgtgaacg gccggggcgt cgaggccgca
     7081 aaggactttc gccggagcgg ggggacgcac atcttcctgg tctcaaagcc ctcctggtcg
     7141 tttggggtcg agccgtcctc gggcgaagac taccgggagg tttttctcgc gacgctccgt
     7201 gtggccgata tggtccggga gaccggactc gtcgtctacc cggtcctcgg cgtccacccg
     7261 gcggagatca accgtctggc ggcgcgtatg agtcttgaag aggccgccgg cgtcatgatg
     7321 gccgggctcg accttgctgc ccgctacgtc gaggaagggg aggccgtcgc cttgaagagc
     7381 ggccggcccc actacgaggt cgcgcctgag gttcttgccg catcaaacgc ggttctctac
     7441 cacgcgctcg aactcggcgc agactacgac tgcgccgtcc agctccacgc cgagagcggg
     7501 ccctgcaccg acgtcgtcga tatggccggc cgggccggta tcccggtcga gcgggtcgtc
     7561 aagcacttcg cgacccccga tacgccgctc atgccctccc tcatcgcgcg gcacgaagat
     7621 atcccggcgc tcgcccgggc ggggcggcat ttcaccatgg agagcgacta catggacgag
     7681 aacgcccgcc ccggcgcggt gatcgggccg aagtcggtcc cgcggttcac gcaccgctac
     7741 ttgaaagagg gattgatcac agaagaggat gcctggcgca tccacgccgc gaccccgtcg
     7801 cgcacttacg gcgtggatat cacgcttccc tgacggaact gcacaccttt ttgacgactc
     7861 ccgtcaaacg atatacgatg tacttaaaag tgcaccacgt accggacgcc ggtgaggtgg
     7921 tggctgcctg cgactccgag ctcctgaatg tcaccctgat gcacggagac gtggaggtct
     7981 gcatcaccgg aggattctat ggcacagaac gtgtcgctga ggaggaggtc aggcgtgctc
     8041 ttctgggtgc gacgaacgcg aacatcatag ggaagcgtgt cgtagccctg gccgtcgccc
     8101 tggggctcgt cagaagggaa gactgcatca tgatcggcga cgtaccccac gcccagatct
     8161 tcgggatgta attcatggcc atacagacga gcatctgccc ccgctgcggg cagccgaccg
     8221 aagaggggct ctgcccgcag tgccgggccg cggatacccg gctgctcgcc tgcgagcccc
     8281 gcgtgaccgc ggtctactgc ccggtctgcg actcgcagag gcgcggcaag acctggtcgg
     8341 atctgcggat gccgcgcgaa gacctcatcg cagagatggc catagcggcg acgagcatcc
     8401 atgaggatgc aaaagacatc cgggtcgccg tccggtcggc agagaccggg ccgaacagga
     8461 cgacctgcac cgtcgacgtg gaggggaccc tctactccgt cccggtccac gggacctgcg
     8521 aggtcgagat cctctggcag aaggaggcct gcgaccggtg cagccgcata tccgggggct
     8581 actacgaggg gatcgtccag gtcagggcga cggacaggaa gatcaacggc tacgagcgcg
     8641 aggtcgcggc aagcatcgcc gatcaggccg aagagtccct ccagcaggcg ggggaccgcc
     8701 tctcgttcat atcggatctc aaagactcta aagacggcgt cgacatcacc gtcggatccc
     8761 agcacctcgg ccaggagatc gcccggatga tcacgggggc tctcggcggg cggtatacga
     8821 cccacccgaa actggtcggc gagaaggacg gtaaagccct ctaccgcgtc acctactcca
     8881 tccgccttcc tttctaccag aagggggacg tggtggtctc ccacggcggc tactttgagg
     8941 tccgcgatat cgacggacgc cggctccggg cctttgatct tgcaaccggc gcgacccgga
     9001 cacttgccga ggatgaggtc gagcgcctga tcgggaacgt tcgcgaggcg gagtccgcgc
     9061 tcgtcgtctt cacgcagccc gacatcgtcg ggctgatgga cccgaagacc taccggaccc
     9121 gggagcagag cccggtgccc tggacgttcc cggtcgaagg gcagccgatc cgggtgctcc
     9181 gcgacgaaga gcaggaccgc ctggtcatcg tcgggtgagg ggccggcatg cgtgcgcgga
     9241 gggtgccggc ggccgttctt gccgatatcg tgggtgcgga gtgggtggac cctgcccgcc
     9301 ggccctacgt ccggggcggc accgcctggg tcccggtccg ggaggggtat ccggccgacg
     9361 cggaccttcc cgaacgggag agctaccggg ggcgcggtta ccaccggatc ggcgatgtcg
     9421 cggtcctccg tggcgacgcg ccgtcggagg ctgagctcgc cgcgatcgtg gagcactgcc
     9481 gcccccgggg cgtcctcctg gtgaagggat acctgggcga tatgcgcatc cccgatgtgg
     9541 agatcctcta cgggaacacg ggcgaggtat gccaccgcga gcagggctac acgttcatcc
     9601 tcgacccggc ccgggtgatg tttgctcagg ggaaccgcaa cgagaaggcg aggatcgcct
     9661 ccctcgtccg gcccggcgag cgggtcgccg atatgttcgc cgggataggc tacttcacca
     9721 taccggcggc cgcaggcggc gcgcaggtcc atgcgatgga gataaacccg atagcctttg
     9781 aatacctgca gcgcaatagt atggctaacc atgttgctga tcgggtcacg gcagagaacg
     9841 gggactcccg gatactcctc tccgggacct acgaccgcat cctgatgggg cacttcgatg
     9901 ctccgtcgat gctcgccgac gccctcgccc acgcccacag cgggagcgtg ctccacgtcc
     9961 acagcatcgg ggatgtgaga ggtgcgatcc gcgacgcgac agcggaagcg ggctttacgg
    10021 cgatggtgac ctcccgtcgg gtgaagaagt acggcccgca tgcgtggcat atggtgcagg
    10081 atgtgacgat atcgtgaaga cgttttcggg aaaaacggtg gtcctcgccg tgacggggag
    10141 catcgcggct gtggagacgg tgaaacttgc ccatgcgctg cgccgccggg gtgctgaggt
    10201 gcaggcggtc atgacggagg cggcgaccgg gatcatccac cccgatgccc tgacctacgc
    10261 gaccgggcgg ccggctataa cccgtatcac cggcctcgtg gagcacatcc tctactgcgg
    10321 cgagggcggg gcggccgacc tcctcctcat cgctccctgc acggcaaaca ccatcggcaa
    10381 gatcgccgcc ggtatcgacg acaccccggc gacgaccttc gcgacgacgg cgctcggccg
    10441 gaggatgccg gtggtggtcg ctccggcgat gcacgagagc atgtaccgcc accccggggt
    10501 cgtggagaac ctcgaacggc tccgctcctg ggggatcgac atcgtcgccc cccggatcga
    10561 ggagggaaag gcgaaggtcg cggatatcga tacgatcgtc ctccacgccg cacgtgcagt
    10621 ctccggccgg ccgctcgcgg ggagacgggt cctcataacg agcggggcgt gcgcagaacc
    10681 cttcgacgac gtccgggtcc tcaccacccg gtcgaccggg cggatgggac gggcgctcgc
    10741 gctcgaggcc ttccggctcg gcgccgacgt gacggtcgtg cacgcgggat cgtttccctg
    10801 cgtccgaaac atccgtgtag cgaccgctgc ggatatgcgc cgggcagtcc tctcggtctg
    10861 ccgcgatggt ggtgtcgata tctacatgag cgcggcggcg atatcggact tcgcccccga
    10921 gcgctgcgag gggaagatac cgagcggctc gcccctcgac atccggctca accccctccc
    10981 gaagatcatc gatgaggtga tggcgacgtg ctgccccgtg acggtggcgt tcaagctggg
    11041 ctggcacgag gaggagcgag cgagaacgat gctcgatgcg ggcgtgaaga tggtcgtcgt
    11101 gaacgctccg ccggcgatgg gggcggcagg ggggacgttc tcgctcatga tggcagatag
    11161 taccattgag gtaacgggga gcaaagagga ggtcgcggcg gcgatatggt caggactgct
    11221 gtagccttct cccccgggca catatccggc tacttcaggc gggtcgaggg gagcgatccc
    11281 cggaccaccg ggagcgtcgg ggccggcgtg gtcatcgacg aaggggtgcg cgcgacggtc
    11341 gtcccggccg caaagaccgg tgtccgggtg atccggcagg gccgcgtgag caccggatcg
    11401 ccgccggtcg agtacgccct cgaccggctg ggggtcacgg cccgcgtcac cacggagtgc
    11461 cggctcccca tcggcgccgg gttcggcctc tcggcggccg cgcttcttgc gacgctcacg
    11521 gcggtaaacc acctcttcga tctcggtctc tccgcggatg aggtggcatc ccgggcccac
    11581 gaggccgagg tcgtccaccg caccggcctc ggggacgtcg ccgcgagccg gtcggggggc
    11641 gtcgtctgcc ggatggggcc cggcatccac gcggatatcg cccgatttta cccggacgaa
    11701 cccctctacg ccgtgagtct cggcccgctc ccgaccgcct cggtcctctc gtcggaggaa
    11761 gcaatggcgc ggatagcctc tgcctacccg gatcgctgcc cccgcgatat cgccgacttc
    11821 tgcttcctct cacggcagtt tgccgagaga agtgggctca tcgcgccgga tgtgcgaaag
    11881 gtgctctctg cctgcgacgc cgcgaagatc ccggcaagca tgacgatgct tggaaacggc
    11941 gtctttgctg caggcaattc cgcagagcgc gtgctttcgg gattcggtga ggtctaccgc
    12001 ctcaacgtcg cacgccgcgg cgcatacttg atcgagatga gaccatgata ccgaaagatc
    12061 atccacgcta ccgctccctc atcgcccgcg aacggctggc acggtgcgcc cgcgagggta
    12121 tcgtcgccct cgaggggctt gccgcccacg ggcgcgggga ggcgtttgac taccttatcg
    12181 gagaacggac gagcgagagt gcagctctcg ccgcccggac ggccgccgcc atgcttctct
    12241 ctgcccggca cccggtgata tcggtgaacg gcaacaccgc agcgcttgcg gcagatgaga
    12301 ttgcggccct ccagaaggca agcggcgcag atgtcgaggt gaacctcttc caccggacgg
    12361 aggagcggat acggctgatc acccggctcc ttgaagagca cggtgtccgg gtcctctccg
    12421 ggaccgcgga acggctcgtc ccgctcgctc acgaccgggc gctctcgctc cgggagggga
    12481 tcggagacgc cgacgtggtc ctcgtgccgc tcgaagatgg ggaccgctgc gcggcgctcc
    12541 gggcgatggg gaagacggtc atcaccatcg atttaaaccc gctctcccgg acagcccgca
    12601 cggcgaccct gacgatcgtc gatgaactga cgcgcgccgt ccctgccatc accgtggcgt
    12661 gcgcctctct atcgcccggc gagggcgagc gcctgatcgc ctcgcttgac aatatctatc
    12721 ttctccgggc ggcgatagat gagatgagag agagattgag ccatgctctg gagtgaggtc
    12781 taccgcccga agcggtgtga ggatatcatc gggcaggatg cggtcatccg ttatctcgtc
    12841 tcgtttgcgg atgcaggaag cgtgccccac atgatcctat cgggcccgca cggcaccggg
    12901 aagaccgtgg ccgtcgagtg cctggcaaaa cggctctacg gcgagaactg ggaggcaaac
    12961 accaccgtcc tcagcgccgc cgatctcctc gggcggggga ggagcgccct tgagacggac
    13021 gagcgctttg cccagatcta ccgaaaggac cagagcctga tcgtcaactt caagtatgtc
    13081 gtgaaatggt atgcctcgat gcgcccgctc gatgccgact tcaaactgat ggtcttcgag
    13141 gatgccgaag gcctgacgtt tgaggcgcag caggccctgc gccgcaccat ggagcgctac
    13201 agcgccacct gccggttcat attcgtcacc acccggccat cggcaatcat ccccgcgatc
    13261 gcgtcgcgct gcctgccgct cttcttcgct ccgcttgaga gcgcgctcat ccgcacccgc
    13321 ctggcagaga tcctcgccgc ggaagcggct acgtgcccgg ccgacgacat cgacctgatc
    13381 gtccacgctg cccggggcga cctgcgacgg gcgatcacgt acctccagat cgttgccggg
    13441 gcgggagaag agatcgacct tgcagaggtg gcgcgctccg agacctcgaa tgtggcaaca
    13501 tccgcgtttg aagcgctccg ggccggcaat tttgccgccg cgcgcagcat cgccgagtcg
    13561 ctgatgatcg agtacgggct cccggcgcgg gaggtcgtcg gcgaactccg gcgggcggtc
    13621 aggcgcgagt acaaccaccc tgcccttgcg atcgcgctcg ccgatgcgga ctgtcagctc
    13681 ggtcacaatg caaacgattt tgtacagatc aatgcacttc ttgcccggat tgcccgggag
    13741 gtgtccagtg aagaaagtac agcagcacta tgatgagatc gctgatatct acgacgatcg
    13801 ctacgatggc aaccgcggga agtactatca cgggcacata tccgaccacg tgatgggcgt
    13861 tctcccccgg ggcgggttcc tcctcgatat cgggtgcggg accgggcttt tcgtgcaacg
    13921 ctacgtcgaa gaagggggtc gggcggtcgg cctcgatata agcccgggga tggtccggca
    13981 cgggcggcag cgctgcccgg cgagcgggtt ctttgtcggg accgctgacg tcctcccgtt
    14041 taaagatgcc acgttcgatg ctcttgcaag cctgctcgcc ttcagttacg ttcccgaccc
    14101 ggaggcgatg ctccgggagt gctaccggat cttaaaaccc ggcggccgga tcgccgtctg
    14161 caccctctcg cggacggtct tcacgagcat cgttcctatc gcctaccagg tgggggagaa
    14221 ggtcggcctg aagaaggtgg gcgtcgggga cttcggcgag cactactaca ccaacagcga
    14281 gattgccagg ctcttttgcg aggcaggatt cgaggatgtc gcggtcagcc ggtgctcgtt
    14341 tgcgcactta aacctcgcag acccggtctt ttacctcgcc cggaaggtgg agcccttcat
    14401 cgaggagaac ctgccatacc tcgcctataa cgtctgtgcg ggcgggaaga agccggaggg
    14461 ttagatcact gtgtgggcgg gatgcgggag tcactccctg ctcccaccca ccgggccgca
    14521 tgggaaggct ctgttttacg atgcagagac gataacggaa tagttatatc ctgcctcccc
    14581 gaactcctcc ccatggagcc ggtcgatatc gtcctgacga aggatacgtt cgaggttctc
    14641 gcatccgaga cccggctcac gatcctgaag accctgaatg aacgcaggat gacgatcacg
    14701 gagctcgcag acaacctcgg cctggcaaag tccaccatcc atcaccacct gcagaagttg
    14761 accggcgccg gttttgtcgc tgccgatggt gacgggcacg cctgggtata ctatgcgctc
    14821 acgccggagg gacgcgccct cctgcagccg cacggcagcg cccggatccg gatcatcctg
    14881 actgcggggc tggcaagcct cgccggaggg gtctgtgccc tcgcggcctt cctgacggcg
    14941 ccagtccggg aagggccggt atttccggga ccgaagggcg gcggcggcct cccggtcccg
    15001 gagggggcgc ctgtggaact gctcgttgcc ggggtcgcgc tcgcagcggt cgggggggcc
    15061 ctcatgacgt atgcctgcgt ccggtggcgg aacacgcgcg cggcggggta cttccctgcg
    15121 gacgaaaaaa gcgcgtagcc tcaatacata gcttttgaaa ccattcgacc gggatcgaac
    15181 ggtttcgccg gtattatcgt ctttctgcga gagagaatcc atgtggatgc ccggggtgta
    15241 cccggtctcg aaggttttcg gccttccccc cgatgcgatc cacgggagag aacgatgaaa
    15301 ggaacgatat ggctattctc cggaataagc atcctgattc tggcgctcat tcttgtagga
    15361 ctgaccgccg ccggcaccgc accccagaac acgacatcaa tctccggcaa acccgttgcc
    15421 ggggatgccg gtatcgccca tcccgatacg ccggacggcg ccggcaacgt ggcggccggg
    15481 aacaatcggt tcgcgttcga cttctaccgg cgcctcgcga ccgacccggc gcacgcggga
    15541 gataacctct tcttctctcc ctacagcatc tcgtcggctc tcgcgatcac ctgcgaaggc
    15601 gcccgcggcg cgaccgccga cgagatcggg gcggtgctgc acctcccgac aaacgagacc
    15661 ctccggcgag aggggtttgc cgcgatcgat accggtctga actgcgcgag cggcaactac
    15721 accctccgca ccgcaaacgc cctctgggcg gagaagaccc attcgttcct cccggagtac
    15781 atcgacgtgg ccgttcgctg gtatggggca aacgtcacga acctcgactt cgtcgagaat
    15841 cccggggggt cgcgggagac gatcaaccgg tgggtggagg agaagaccga agagaagatc
    15901 cgcgacctcc tgccgccggg ttcgatcgac tccatgaccc ggatcgtgat cacgaacgcg
    15961 atctacttca aaggaacctg ggccgagcag ttcgaccccg acgggacgac cggggaggag
    16021 ttccgggtcg gaccgaacga gacggtgacc gtcccgatga tgcacgggaa aaccatctat
    16081 ccgtacgccg agaccgatac cctccgggtg ctcgagatgc cctacgcgca cggaaacgaa
    16141 accgaactcg cgatgctcgt cctcctcccg aaggaagata acctgacggc tgcggaggag
    16201 gccctggaca cggagaggct tgccggcctg caggagtccc tggtctcaca gaacgtcagg
    16261 gtcttctttc cgaagttcac gctggagacc gggtatcctc tagcgggagt gcttgcggcg
    16321 atggggatgc cgacggcgtt ctcccccgat gccgacctct cggggatgga cggcacgaga
    16381 aacctcttca tcaccgggat ctaccacaag gcgttcgtgg acgtgaacga ggagggcacc
    16441 gaggccgcgg ccgcgaccgg ggtcataggg gggaagggtg tcacacccgt cttccgtgcg
    16501 gaccacccgt tcgtcttcct catcgtcgag aagaactccg gcacgatcct cttcgcagga
    16561 agggtcgtca atcccgaaag cccgtgaacg agaggctgtt ctctcccctt ttgtcggcaa
    16621 cggataccgg ggcaactgtc gcctacccgt ttcatgaagg gatggtaggt tgcccggcgc
    16681 cgtctgtgcc gccgagcaaa gagaggagca tcgcgacctc ttctctgagt gcggggatat
    16741 cgcggttgac gaccgcccag acttcctcag ggtccacgcg gaagtatgcg tgaaccagaa
    16801 cgttccgaag accgatcacc tgtttccagg gaacgccggg agaagcgctt ctaaggtcag
    16861 ggggaggcgg gcggctgctt ccccgatgat ccggagatgg tggaccatcc agacctgcac
    16921 cagttcgtcc tgcaagaaag catctctgcc ccggacggca taccgctcaa tctccttgat
    16981 cgcttcctgg atgtcgagga gccggtcgat cccatccctc atatcggcac tgcctctttc
    17041 aagatccgct ttttgtaccg ttccttgagc gccgcttccg tcaccacgtc cacccggcac
    17101 cccagggcct cctccaggtc ctggatgagt gcgatgtgat caaggaggct cctgcccggt
    17161 tccagctcga caaggaggtc gaggtcgctc tccgggcctg cctcgccccg ggcgaccgac
    17221 ccgaagaccc ggaggttcct ggcaccgtgt cgcccggcta tagcgagaat cctggaccgc
    17281 ttctcccgga ttagatccac tgtgacaggc atggctgttc aatatcttct atcgggagct
    17341 ggcaatataa atctgccctc aatggtcgca ccgctaaaaa gatcccgggc ataccccgaa
    17401 ctatggtctt ccgaagaggg tgaaatccgg ctggcatccc cgccttcttg tactgctgat
    17461 tgggaatcga tgcactaaag agagaaagta tctctccatc gctcaataag ccgccctgtt
    17521 tcctcacgac gaccggagcc gggagaggat ccggagcttc tttgcaagcg ccgcctccgc
    17581 cgcccgctcg acccccgcct tcatctccgc aaagtctctc ttgtcctcgt ccacgacctt
    17641 cttgaccggg gtgtaggccg actccctgaa tcggggcgag aagtcgcgga tctcgccgcc
    17701 gaccgagaga accgattccg gtgccccttt ctcgacgtag ccgacctctt tcatcgcgac
    17761 acctgcggac gcaacgaccc ggcggatatc gggcgcgacc tcgggcggtg cgacgacgag
    17821 aagcgcatcg agcgatacgc cgaggtagtc gatctcgagc gcgtcgagca tggcaaggac
    17881 cttcggttgc acgagcgtgc ggagttcgtc ctcctcgacg acgatccggc acccggcagt
    17941 ctccgccatc tcgtaggcgt cgccccggag gccgccgttc gtgacgtcgg tca
//
LOCUS       DAOSII010000028        17799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_3391_length_17799_cov_56.496450, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000028 DAOSII010000000
VERSION     DAOSII010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 17799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17799
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3391_length_17799_cov_56.496450"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PLV90_03585"
     CDS             <1..569
                     /locus_tag="PLV90_03585"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOF96225.1"
                     /translation="VYGYPAAEPAGWRSARLRCRREGAYTTAGRTLANFRGLRASLSS
                     APSRIIEEDSSMQPDNEQLIDDFLAHADDLGDDIVADVKEMLGLVPFIFTILRDRPDV
                     FALSTIADYRTSRPDSLDSKTAELIAVAAAASAGADSCLKVHIGAALKEGASRDEVLD
                     TLLIAAMIGKTRVLASSLRQFREVCGEE"
     gene            651..1226
                     /locus_tag="PLV90_03590"
     CDS             651..1226
                     /locus_tag="PLV90_03590"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOF96226.1"
                     /translation="MSQTKATLRAQAREARSLLSREEAAIQSATIERHLLDLLDGFET
                     VMVYTAKPQEVDTSGLIADLNRRGVRVVVPIIERETVSLRLSYLPDPAVLVPSTFNVP
                     EPIGHELPARPGDIEAVVIPMLAFDIEGNRLGYGAGYYDRFLCRYPRPQRIGIAFSCQ
                     RAESIPADENDVKMDCIVTETGIRRYNRRGP"
     gene            1287..3710
                     /locus_tag="PLV90_03595"
     CDS             1287..3710
                     /locus_tag="PLV90_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HOF96227.1"
                     /translation="MANTESVEVTIKEAAHEDAGRGIARLSIDTMKVLGLVSGDVVEV
                     EGRQKAATLIWPGFPQDTGKAILRIDGSTRSNVGAGIDDKVRIRKTEAGYAKKVTIQP
                     TQPIRLVGGEQYLGRLLRGRPVTEGQHVRVNILGNPLTFVIAKVAPKGIAIVTENTEI
                     ELKETPYEPKEGKREVAGDIHYEDIGGLDRELQLVREMIELPLRHPELFERLGIEPPK
                     GVLLYGPPGTGKTLIAKAVANEVDAHFITLSGPEIMSKYYGESEERLREVFEEAQENA
                     PSIIFIDEIDSIAPKREEVKGEVERRIVAQLLALMDGLKTRGQVVVIAATNLPDMIDP
                     ALRRGGRFDREIEIGIPDTKGRQQIFQIHTRGMPLAEDVNLDEYARSTHGFVGADIAL
                     LAKESAMHALRRIIPQIKIEEEIPAEIIDELRVTNEDFLEAHKHVEPSAMREVLVEIP
                     DVKWEDVGGLEDVKAELAEAVEWPLKYPEIFASLDTEPPRGILLFGPPGTGKTLLAKA
                     VANESESNFISVKGPELLSKWVGESERGVRQVFRKARQAAPSIIFFDEIDALMPKRGT
                     YIGSSHVTESVVSQILTELDGLEELNNVVVLGATNRPDMLDDALLRPGRLDRMIYVPP
                     PDREGRKKIFEVYMRNKEILANDVDIDELVDRTEGYVGADIEALVREAKISAMREFIA
                     AMGKKSDDERKKAIGNVRITKRHFEEALSRVRGTLDIDRLEENERQSWQILYNQEQRS
                     ALEDAISTINRARMRETGKSEQETRDLTQTLKDEVYRRKKDFGEIKRLTRELKTRIER
                     PLPQAGMAF"
     gene            3794..4246
                     /locus_tag="PLV90_03600"
     CDS             3794..4246
                     /locus_tag="PLV90_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOF96228.1"
                     /translation="MSDSPADIFEQLNELMKRLMEQGLKEQGDQNRSFAYGFKIVLHD
                     AGKTETPAKEADPVAEPEKASSEGTIEPIAEMYTTDDDVTVAIDLPGFEKENIHLSLI
                     NGTLTIIAGSSQLTSVEVPAVDAGSMQSNYKHGVLEVRFSRGKTIKID"
     gene            4578..5675
                     /gene="fbp"
                     /locus_tag="PLV90_03605"
     CDS             4578..5675
                     /gene="fbp"
                     /locus_tag="PLV90_03605"
                     /EC_number="3.1.3.11"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphate aldolase/phosphatase"
                     /protein_id="HOF96229.1"
                     /translation="MVKTTVSVIKADVGSFPGHSRTHPKLLEVAARRLKEAEGSTIID
                     SYVTHCGDDLELIMTHTKGEDNEEIHKLAWDIFMECAELAKEMKLYGAGQDLLADAFS
                     GNVKGMGPGVAEMEFEERGSDPVLVFMADKTEPGAWNYFLYKIFADPFSTSGLVIDPS
                     MHQGFTFEVHDVMEKRRILFNTPEESYSLLAYIGAPSRYVIKYVRKKDGTVGASTSTQ
                     RLNLMAGRYVGKDDPVMVVRAQSGFPAVGEVIDPFRTPILVAGWMRGSHHGPFMPVGV
                     GDANCTAFDGPPRVICLGFQVNNGKLIGPADMFDDPAFDRVRDRCCELSDVLRAHGPF
                     EPHRLSLEEMEYTTLPGVEKQFKDRWEDLPE"
     gene            complement(5863..6183)
                     /locus_tag="PLV90_03610"
     CDS             complement(5863..6183)
                     /locus_tag="PLV90_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96230.1"
                     /translation="MSGEVYQAQVLRSFFDTITGTDRNLTRIYMCVVSLAKLRMEPPE
                     RMTFLVDQLRKSKQKRELSVDIIDYMCDAARDLELGTVRTAFGVKDINEVADEFSGIS
                     LDSL"
     gene            complement(6284..7453)
                     /locus_tag="PLV90_03615"
     CDS             complement(6284..7453)
                     /locus_tag="PLV90_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOF96231.1"
                     /translation="MITLAIAGKPNCGKSTFFRAATLAQAEIANYPFTTIDANHGVAY
                     ARTTCPCREMRVPCENCLDGVRFVPIGLIDVAGLVPEAHKGRGLGNQFLDNLRQADAI
                     LQIVDASGGTDAEGNPVGIGSRDPEKDIEFLQYEFSMWMYGILSRNWAKLQRQAQARD
                     FSLAAAIADTFAGLGVTLEQIRDATDEAGIDLRTAGEEDLIRFCRELMAISKPMLIVG
                     NKADLAPQECLDRLAKHDVIFASAAGELALRMAAEGKFIRYLPGDQSFTPNPEANLSA
                     AQQAGLAKVAEFMRRFDGTGIQRALDAAVFDLLDRIVVFPVEDEHKLTDSKGRVLPDA
                     FLMKRGSTPRDLAYQVHTDIGEGFLYAIDARTGMRVKDTLELKNGDIIKIVSVRK"
     gene            7662..8252
                     /locus_tag="PLV90_03620"
     CDS             7662..8252
                     /locus_tag="PLV90_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HOF96232.1"
                     /translation="MGFVTRNTYYFDAPGAQNTADAARFAVERAQELGAKKIVVASTG
                     GRTALTLLDAVKGTDLRLIVVTHVVGFSKPGEWEFSREAAETLRAAGATIVTGTHALS
                     GLERAISRSPGLGGSSRTEAIAEALRRVVAIGLKVAVECVLIAADQGVVGVDEEVIAV
                     GGTLSGADTVCVIRPAHTATFFNLQVREIVAMPRNR"
     gene            8255..9082
                     /locus_tag="PLV90_03625"
     CDS             8255..9082
                     /locus_tag="PLV90_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96233.1"
                     /translation="MTLGSLSGAAGLLRRHPVLWSVGIVMGVLAVIDLVIPLYGGTFY
                     AEPLALLQALVMPFLAGGVYGTIRANSFSAGEFLQSGKTYYFRILLPALLIFFAVILT
                     AFLLAIPLTLLGAGAASGTMPLLLGVLVSIVFFTFFYDTVAVFEETNVFESIRRSVEF
                     VMNNLGNVLVFYLVNIVVLVGIGFAAFVAIAALLVDRLEPLTRLDPAELQAVTPQDLL
                     GLIGTEGIWTIAAVYAVVIVLFAAFLYAYKASFFRDHAASVPIQQGEYDEKGRWYKY"
     gene            complement(9299..10495)
                     /locus_tag="PLV90_03630"
     CDS             complement(9299..10495)
                     /locus_tag="PLV90_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HOF96234.1"
                     /translation="MTKPPGLVLASVLTLAFIGLAPLLQPAWLLTLLLIPFSLVLFLI
                     RDTRYVSLSIIVLAALYGFGWLSTFVFTCTLGIVVIGELAFRFTGGKQASYLYHLAAA
                     VGVSLGVMLYLRYSAPLVVMMGVVAAVLLRAALRGRDDVLMVEALGVAMTMFLFEEIN
                     FQVDLTILVVAAVIAFGFGYSSYRLRVADVSGLFSGAMIGIILIVFAGVPWFLIMLTF
                     FIVGAGATRYRYGDKEQLGVAQEHAGVRGYFNVFANGLVATGAAILYGVTGNPAFAAL
                     FMGSVASAAADTAASEIGVTGKTPYLITTLQPVPRGTNGGVTLRGEMAAVAAAALVAL
                     VAWMLGVADPRMAVVTVAAGFVGTNVDSLVGATLENSGRIGNSGTNLAATFFGGVFGM
                     LVYLVT"
     gene            10659..13250
                     /locus_tag="PLV90_03635"
     CDS             10659..13250
                     /locus_tag="PLV90_03635"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOF96235.1"
                     /translation="MSPSHQLPKNYDIEEVERRWQDTWRDEENYFDPDSTKPRFIIDT
                     PPPYPTGNFHIGNALNWFYIDFVARYKRMRGFNVMFPQGWDCHGLPTEVKVEETYGIT
                     KNDVPRERFREMCRDLTLGNIEKMRATMRRLGFSTDWSHEYVTMLPEYYKKTQASFLQ
                     MLKAGDIYQSEHPVNFCTRCETAIAFAEVNYAPRTTTLNYFDFDGVEIATTRPELLAA
                     CVAVAVHPGDERYRGMKGATLAVPIFGHRVPVIEDVAVDPEFGSGAVMICTFGDKTDV
                     YWWKQHSLDLRKAIDRQGVMTAVAGRYAGMSSDACRKAILEDMEEAGILKRREELEQR
                     VGTCWRCKTPIEILSERQWFVRVHQKEILEAAKEITWLPEHMFTRLENWTESMEWDWC
                     ISRQRIFATPIPVWFCDACGGTILPAMEDLPVDPTADMPKAPCPRCGGTRFTGEKDVL
                     DTWMDSSISVLHVTGWDGSSRPPLFPAQLRPQGHDIIRTWAFYTILRSKALAGSHPWE
                     QILINGMVLGDDGFKMSKSRNNIISPEEVVGKYGADALRQWGAGGAAIGSDIMFNWND
                     VVAASRFQTKLWNIFRFVMGHLERGADPAAPVTELIDRWLLVRLSDTVAEATAAMEGY
                     QFDRALKAVREFAWNTLADDYIEIAKGRLYAEEGGRASAASALRTTMDVLCRLLAPII
                     PHFAEECYHILTGGSVHKEAWPDFTYADDEARRHGDLLVKTVAELRRYKHDKGMALNA
                     PLGHVTIYAPEPVDDTGDAGRALAADAHWRTGTPRLEEVADGVDFNMAVIGPALRKQA
                     RGFMEAVRALPPDLLKNPPAAVTVDGEEVFVPPGSFTPKIAYQVAGEEVDVLTLGDVV
                     VTIGHSS"
     gene            complement(13207..13824)
                     /gene="tmk"
                     /locus_tag="PLV90_03640"
     CDS             complement(13207..13824)
                     /gene="tmk"
                     /locus_tag="PLV90_03640"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOF96236.1"
                     /translation="MLITIEGIDGSGKSTLIARLRELIADLDPLFTREPGATWVGESV
                     RRAVAERMDPITEALLFCADHAAHIDGVIRPALDEGRLVVSDRYSDSRFAYQPVVLAG
                     ILPDPLLWLRRIHDGWSVRPDRTFLLVLPIEDAVARLDTEKKREYFENAAILARVQEN
                     YLDLAASDPARFVVVDALLPKEDVARFIADEIRTNVLSSRRHPRA"
     gene            complement(13818..14498)
                     /locus_tag="PLV90_03645"
     CDS             complement(13818..14498)
                     /locus_tag="PLV90_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOF96237.1"
                     /translation="MELILAVDLAGGLVVHGRSGDRAGYRPLTWGLAPSAEPETYIAA
                     LEPRYIYIADLESIQGRTPQDELVRRCTALVERCYLDRGIRSPADCTAVAGVTPVVGT
                     ETAAKAIEDLAAYRAGYLSIDVKGGRVLPWGIRPEEMLCHASEFSFEGCIILNIGAVG
                     TQRGLVREDLEEMRACYPGRLLYGGGVAGTDDIRLLDDAGFDGAIIATAVHRGTVPLE
                     WVRRGHPC"
     gene            14543..15232
                     /locus_tag="PLV90_03650"
     CDS             14543..15232
                     /locus_tag="PLV90_03650"
                     /EC_number="4.2.3.153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HOF96238.1"
                     /translation="MQLLVSPSSIEEARSSLSADIIDVKKPSEGSLGANFPWIIREIK
                     TLAGRKPVSAAIGDNEYKPGTAALSAYGAAHAGADFIKVGLMFDGTDHAREVIGAVTT
                     AVKEEFPHKYVVIAAYADFQRMDTISPLAISPLVADAGADIAMIDTGIKDGKSLFEFM
                     DEEALTRFTEQNRGFGLKTALAGSLRFEDLDALKRIDPEIIGVRGMVCGGDRSATIRA
                     ELVEKAMMMLR"
     gene            15236..16702
                     /gene="guaB"
                     /locus_tag="PLV90_03655"
     CDS             15236..16702
                     /gene="guaB"
                     /locus_tag="PLV90_03655"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOF96239.1"
                     /translation="MYIEKMQMETTFTFDDVLLEPAESWVEPDEADVRSRFSRNIPLY
                     IPLVSAAMDTVTESVMAITMAREGGIGVIHRNMPPEREVAEIRIVKQAEDLIEREVVA
                     VGPDSTVADVERVMKLHGIGGVPIIEGSKVIGIVSRRDIRAILPRRGEAKITEYMTRK
                     LITASEDVTAESALETMYANKVERLPVVDGEGCLVGIITMRDILEKRQYPRANRDANG
                     KLRVAAAVGPFDFNRAMLLVDGGVDALVVDCAHGHNMNVVNAVREIKASVAIDVVAGN
                     IATRQAAGALVDSVDGLKVGIGPGSICTTRIVAGVGVPQVSAIASVAEVARDAGVPVI
                     ADGGIRYSGDIAKAIAAGADSIMAGSLFAGTDEAPGRITTIKGRRYKQYRGMGSLGVM
                     SGGESSDRYFQKKEIGKTKFVPEGVEGVTPYVGRVSDVIYQLVGGLKSAMGYTGSKTI
                     GDLKKNGKFLRITSAGYGESHPHNIMITDEAPNYRLLD"
     gene            16737..17360
                     /locus_tag="PLV90_03660"
     CDS             16737..17360
                     /locus_tag="PLV90_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HOF96240.1"
                     /translation="MLEGDEVSRLLDILGNRNRRRIIELLRQKPCFVTEISERLVLSP
                     KAVIEHLQMMEREELLVSCQDERRRKYYYLSHDINVIVNLQKQDGITPPSVEEDQRAR
                     FTRNLSALRRMILARDELLENLEQLEREIESRFVEVVRTGGGFTADDGDLEIILALSH
                     AALTLAELEEVSSLSTDELKQRLGSLMHMGIVRRINDRYRIRGTYGE"
     gene            17350..17763
                     /locus_tag="PLV90_03665"
     CDS             17350..17763
                     /locus_tag="PLV90_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96241.1"
                     /translation="MANNPYDEMLKNIAQLMGKILSEMPLQDPKIIGFTIISGVPEGA
                     PYLDLAGDEGAGFEVIEGDDCIYITATVNAQVQGAPYVTFQRESVTLCTGGDEETIIG
                     LNCEIDIPHSFYNVQHGVIDAVCRKRSTPGEGARP"
BASE COUNT         3585 a         5525 c         5672 g         3017 t
ORIGIN      
        1 aggtatacgg ctaccctgca gcggagccgg cagggtggcg atctgcccgc ttacgctgcc
       61 gccgggaagg agcgtacacc actgccggcc ggactctcgc gaacttccgt ggccttcgcg
      121 cgagtttatc gtcggcgccg tccaggatca tagaagagga cagttccatg caacccgata
      181 acgagcaact gatcgacgat tttctcgctc acgccgacga cctcggcgac gatatcgtgg
      241 cggatgtcaa agagatgctc ggcctcgtgc cgttcatctt caccattctc cgtgaccggc
      301 ccgatgtctt cgcgctctcc accatcgccg attaccggac ctcccgcccg gattctctcg
      361 attcaaagac cgcggagctc atcgccgttg cagctgcggc gtctgccggc gccgacagct
      421 gcctcaaagt ccatataggg gccgccctga aagaaggcgc atcccgcgac gaggtcctcg
      481 acaccctcct gatcgccgcg atgatcggaa agaccagggt cctcgcatcc tccctgcgcc
      541 agttcaggga ggtctgcggc gaggagtaga cgccgggggc gtagcaggcg cccgcacccg
      601 tgccggctca ggcacccttt tatggtctga ttgagccaaa acttcctgca atgagccaga
      661 caaaggcgac cctccgggca caggccagag aggcccgctc ccttctctcc cgcgaggaag
      721 ccgccatcca gagtgcaacg atagagcggc acctcctcga cctcctcgat gggtttgaga
      781 ccgtcatggt ttacaccgca aaaccccagg aggtcgatac ttccggcctt atcgccgact
      841 taaaccgccg gggcgtacgg gtcgtcgtcc ccatcatcga gcgggagacc gtgagcctcc
      901 gcctctccta tctgccggac cccgcggtcc tcgtcccgag cacgtttaac gtgcccgaac
      961 cgatcggcca cgagctcccg gcccggccgg gggacatcga ggccgtcgtc ataccgatgc
     1021 tcgccttcga catcgaaggg aaccggctcg gctacggtgc cggctattac gaccgcttcc
     1081 tctgccgcta tccgcgcccg cagaggatcg gcatcgcgtt ctcctgtcag agggcggaga
     1141 gcatccctgc cgacgagaac gacgtgaaga tggactgcat cgtcacagaa acggggatac
     1201 gtcggtacaa caggagggga ccatagaaaa atataaatat ttgtttacac ttacctatgg
     1261 taaacataaa ggaggagatt gttcatatgg ccaacaccga atccgttgaa gtgaccatca
     1321 aggaggcggc tcacgaagac gccggccggg ggatcgccag actgagcatc gacaccatga
     1381 aggtgctcgg cctcgtcagc ggcgacgtcg tcgaggtcga ggggcgccag aaggcggcga
     1441 cactcatatg gccgggcttc ccgcaggata ccggcaaggc gatcctgcgc atcgacggca
     1501 gcacccggag caacgtcgga gccgggatcg acgacaaggt ccggatcagg aagaccgaag
     1561 ccggatacgc aaagaaagtg accatccagc cgacccagcc catccgcctc gtgggcggcg
     1621 agcagtacct ggggaggctc ctccgcggcc ggccggtcac ggaagggcag cacgtccggg
     1681 tcaacatact cggaaacccg ctcacgttcg tcatcgctaa agtggcaccg aaaggcatcg
     1741 ccatcgtcac ggagaacacc gagatcgagc tcaaagaaac cccgtacgag cccaaagagg
     1801 ggaagcgcga ggtggcgggc gatatccact acgaagatat cggcggcctg gaccgcgaac
     1861 tgcagcttgt ccgcgagatg atcgagctcc cgctccggca ccccgaactc ttcgagcgcc
     1921 tcggcatcga acccccgaaa ggcgtcctgc tctacggtcc tccggggacg gggaagacgc
     1981 tgatcgcaaa agcggtggca aacgaggtgg acgcccactt catcacgctc tcgggccccg
     2041 agatcatgag caagtactac ggcgagtccg aagagcgcct gcgggaggtc tttgaggagg
     2101 cgcaggagaa cgcgccctcg atcatcttca tcgacgagat cgactcgatc gcgcccaagc
     2161 gcgaagaggt gaaaggcgag gtcgagcgcc ggatcgtcgc ccagctcctg gccctgatgg
     2221 atgggttgaa gacccgcggc caggtagtgg tgatcgccgc aacgaacctt cccgacatga
     2281 tcgaccccgc cctccggcgc ggcggccggt tcgaccgcga gatcgagatc ggcataccgg
     2341 acaccaaggg gagacagcag atcttccaga tccatacgcg cggcatgccg cttgccgaag
     2401 atgtgaacct cgacgaatac gcccgctcca cgcacggttt cgtcggggcg gacatcgcgc
     2461 tgctcgcaaa ggaatcggcg atgcacgcgc tccgccggat cataccccag atcaagatcg
     2521 aagaggagat ccccgccgag atcatcgacg agctccgcgt gacgaacgaa gacttcctcg
     2581 aagcgcataa acacgtcgaa ccgagcgcga tgcgcgaggt gctcgtagag atacccgatg
     2641 tcaaatggga ggacgtcggc ggcctcgaag atgtcaaggc cgaactggcg gaggccgttg
     2701 agtggccgct caaatatccg gagatattcg catcgctcga caccgaaccc ccccggggca
     2761 tcctgctctt cggcccgccc ggaacaggaa agacactcct tgcaaaggcg gtcgcaaacg
     2821 agagcgaaag caatttcatc tccgttaaag ggccggaact cctctcaaaa tgggttggag
     2881 agtcagaacg cggtgttcgc caggtattca ggaaagcaag gcaagctgcg ccgtcgatta
     2941 tctttttcga cgagatagac gcactcatgc caaagcgcgg aacttatata ggatcatcgc
     3001 acgtaactga aagtgtggta agccagatcc tgacggagct cgacggcctt gaggagctga
     3061 acaatgtcgt ggttctcggc gctaccaacc gcccggacat gctggacgat gcgctgcttc
     3121 gccccggcag attggaccgc atgatctacg tcccgccgcc cgaccgggaa ggcaggaaga
     3181 agatcttcga ggtgtacatg aggaacaagg agatcctcgc aaacgacgtc gacatcgacg
     3241 aactggtcga caggaccgag ggttacgtcg gcgccgatat cgaggcactg gtccgcgaag
     3301 caaagatctc cgctatgcgc gagttcatcg ccgccatggg caaaaagagc gatgacgagc
     3361 ggaaaaaggc gatcggcaac gtgaggatca cgaagaggca cttcgaggag gccctctccc
     3421 gcgtgagagg cacgctcgat atcgaccggt tggaggagaa cgaacgccag tcctggcaga
     3481 tcctctacaa ccaggagcag cggtcggcac tcgaagacgc catctcgacg atcaaccgtg
     3541 cccggatgcg ggagaccggg aagagcgagc aggaaaccag agatctcacg cagaccctca
     3601 aggacgaggt ctaccggagg aagaaggact tcggtgagat caaacgcctc accagggagt
     3661 taaagaccag gattgaacgc ccgctgcccc aggcgggtat ggcgttctga cgggagcgcc
     3721 ctcgacccat gaggcgcttc cttcccgggg gcatccggcc cccgccgaca gggacaaaga
     3781 caacgtgatg tacatgagtg acagcccagc agacatcttc gagcaactca acgaattgat
     3841 gaaacgcctc atggagcagg ggctcaaaga gcagggagat cagaacagat catttgccta
     3901 cgggttcaag atcgtcctcc acgatgccgg caagaccgaa accccggcaa aggaggcgga
     3961 tccggtagca gagccagaga aagcatcttc cgaaggaacc atcgagccga tagcagagat
     4021 gtacacgacc gacgatgacg taacggtggc aatcgatctc ccgggtttcg agaaggagaa
     4081 cattcacctg tcactcatca acggcactct gacgatcatc gccggcagca gccagctgac
     4141 ctcggtggag gtcccggccg tggatgccgg ttcgatgcag tcgaactaca agcacggcgt
     4201 cctggaggtc aggttctcgc ggggcaagac gatcaagatc gattgatcgg cgggcaacct
     4261 cccggagagg ccgcacccca cccctcttct tgtcgtatcc gggcctcccg acgagggcac
     4321 cagagaatct gctcacggcg atgcccggac cgttcataga cctgatttcg acgacgaaag
     4381 cgatgagcaa catcaaatca tccccggaat agccgccggg cgaatctgcc acggaggatc
     4441 ggtttacggg gggagagagc ctatccgtaa gatcggttgc aggagccaac cctttttcta
     4501 ggcagcaaca cttacatata cacgaatacc tatctagtag tcttgagggc tacacctttc
     4561 caggcaatgg tgattaaatg gttaagacca ccgtatccgt gatcaaagca gatgtcggca
     4621 gttttccggg gcactcccgc acccacccga agctcctcga ggtggccgcc cggagactta
     4681 aagaggcaga gggcagcacc atcatcgact cctacgtcac ccactgcggt gatgaccttg
     4741 agttgatcat gacgcacacg aagggcgagg acaacgagga gatccacaaa ctggcgtggg
     4801 atatcttcat ggaatgtgcc gagcttgcga aagagatgaa actctacggc gccggccagg
     4861 acctgctcgc agatgcgttc agcggcaacg tcaaggggat ggggcccggc gtcgccgaga
     4921 tggagtttga agagcggggt tctgacccgg tgctggtctt catggccgat aagaccgagc
     4981 cgggggcgtg gaactacttc ctctacaaga tcttcgccga ccccttcagc acatccggcc
     5041 tcgtcatcga cccgtcgatg caccaggggt tcaccttcga ggtccacgat gtcatggaga
     5101 agcgcaggat cctcttcaac acgcctgaag agtcctacag cctccttgcc tacatcggag
     5161 caccgagccg ctacgtgatc aagtacgtcc ggaagaagga cggcaccgtc ggggcctcca
     5221 cgagcaccca gcggttgaac ctgatggccg gccgctacgt cggcaaagac gatccggtca
     5281 tggtcgtcag ggcgcagagc gggttcccgg cggtcggcga ggtcatcgac cccttcagga
     5341 cgcccattct tgtggccgga tggatgcgcg gttcgcacca cggaccgttc atgccggtag
     5401 gcgtcggcga tgcaaactgc accgcgttcg acggaccgcc ccgggtcatc tgcctcgggt
     5461 tccaggtcaa caacgggaag ctgatcgggc cggcggatat gttcgacgac ccggcgttcg
     5521 accgcgtccg cgaccggtgc tgcgagctct ccgatgtgct cagggcccac gggccgttcg
     5581 aaccgcaccg cctctcgctt gaggagatgg agtacaccac cctccccggc gtcgagaagc
     5641 agttcaaaga tcgctgggaa gatctcccgg agtaacccct ttttgcagga caccgggagg
     5701 cctctctacc tccccggtgt cgctcgcgcg caccgccggc tgcagaccgt cgtctctcgc
     5761 agaggcttat tgaccattca ggccccggtg ccgccggagt ttgccgggga gacggtcccg
     5821 ccgctcgcga gaaggactat ccggcaaccc ggggtgggaa gatcagagcg agtcgaggct
     5881 gatcccgctg aactcgtcgg cgacctcgtt gatgtctttt acgccgaacg cagtccggac
     5941 cgtgccaagc tcgaggtcgc gcgccgcatc gcacatataa tcgatgatat cgaccgagag
     6001 ctcgcgtttc tgtttggact tgcggagctg gtcgacgagg aacgtcatcc tctcgggagg
     6061 ctccatgcgg agcttggcga ggctcacgac gcacatgtag atccgggtca ggttcctgtc
     6121 cgtcccggtg atggtatcga agaagctccg gaggacctgc gcctggtaga cctcgccgct
     6181 catggtgatc ggatattccc cggtgcaata ttaaagcctg ccgatttgtg ttcgccggga
     6241 agggcacaaa gagaggggat ccgccccttc ccctctaccg ggctcacttc cggacgctga
     6301 cgatcttgat gatgtcgccg ttcttcagtt cgagtgtatc tttgacccgc atgccggtcc
     6361 tcgcgtcgat ggcgtagagg aacccctcgc cgatatcggt gtggacctgg taggcaaggt
     6421 cgcggggggt cgacccgcgc ttcatgagga aggcatcggg gagcacccgg cccttgctgt
     6481 cggtgagttt atgctcgtcc tcgaccggga agacgacgat ccggtcgagg agatcgaaga
     6541 ccgcggcatc cagcgcccgc tggataccgg tgccgtcgaa cctccgcatg aactccgcca
     6601 cctttgcaag tcccgcctgc tgcgccgcgc tgaggtttgc ctccgggttt ggcgtgaaac
     6661 tttggtcccc ggggaggtaa cggatgaact tcccctccgc ggccatccgg agcgcaagtt
     6721 ccccggcggc gctcgcgaag atgacgtcgt gctttgcgag ccggtccagg cactcctgcg
     6781 gcgccaggtc ggctttgttc ccgacgatca gcatcggttt gctgatcgcc atcagttccc
     6841 ggcagaaccg gatgaggtcc tcctccccgg cagtccggag gtcgataccc gcctcatccg
     6901 tggcgtcgcg gatctgctcg agcgtcactc caaggccggc aaacgtatcg gcgatcgcgg
     6961 cggcaaggga gaagtcacgt gcctgggcct gccgctggag tttcgcccag ttgcgcgaga
     7021 ggatgccgta catccacatc gagaactcat actggaggaa ctcgatgtcc ttctccgggt
     7081 cgcgcgatcc gatgccgacc gggttcccct cggcgtccgt cccgccgctg gcatcgacga
     7141 tctggaggat tgcgtccgcc tgccggaggt tgtcgaggaa ctggttccca agccccctcc
     7201 ccttgtgcgc ctcaggcacg aggcccgcca cgtcgatcag gccgatgggg acgaaccgga
     7261 ctccgtcgag gcagttctcg cacgggacgc gcatctcccg gcaggggcag gtggtcctcg
     7321 cgtatgcgac gccgtggttg gcgtcgatcg tcgtgaacgg atagttggcg atctctgcct
     7381 gggccagggt cgccgccctg aagaatgtcg atttgccgca gttgggtttt cctgcaatgg
     7441 ccagtgtgat catgagcaac tcctgttata ctgcaccgtt cggcgtgaga gtagtgttgg
     7501 gttatgcacc atttatggat tgtgcgcagg ggatctgccg tttgccggaa ccggtgatct
     7561 ctccgtcgcc cgggatcccg cacattgcac cgcctccctt gaaaaccacg ttccagcgcc
     7621 ggaacggggg attcaataag gagcgggaac aacatgactg catgggcttc gtaaccagaa
     7681 atacctacta cttcgatgcc ccgggtgcac agaacaccgc cgacgccgcc cggttcgccg
     7741 tcgagagggc gcaggagctc ggcgcaaaaa agatcgtcgt cgcgagcacc ggcggccgga
     7801 cggcgctgac cctcctggat gccgtgaagg ggacggacct tcgcctgatc gtcgtcaccc
     7861 acgtggtcgg tttctcaaag ccgggcgagt gggagttttc ccgggaggcg gcagagaccc
     7921 tccgggcggc gggcgcgacg atcgtcaccg gcacgcacgc gctctcgggt cttgaacgcg
     7981 cgatctcccg ttcgccggga ctcggcggga gctcgcgcac cgaggcgatc gccgaggcgc
     8041 tgcggcgcgt cgttgccatc ggcctgaagg tcgccgtgga gtgcgtcctc atcgcggccg
     8101 accagggtgt ggtcggcgtc gatgaagagg tgatcgcggt gggcggcacc ctgagcggcg
     8161 ccgataccgt ctgcgtcatc cgcccggcgc ataccgcaac gttctttaac ctgcaggtcc
     8221 gcgagatcgt cgccatgccg aggaaccggt gaatatgacg ctcggatcgc tttccggcgc
     8281 cgccggactc ctccggcggc accccgtcct ctggtcggtc ggcatcgtca tgggggttct
     8341 cgcggtcatc gatctcgtca tccccctcta cggcgggacc ttctacgccg aacccctggc
     8401 actcctgcag gccctcgtga tgccgtttct ggccggcggg gtctacggca cgatcagggc
     8461 gaactcgttc tccgccgggg agttcctgca gtcgggcaag acctactact tcaggatact
     8521 cctgcccgcg ctgctcatct tctttgcggt catcctgacg gcctttctgc tcgcgatacc
     8581 gctcacgctc cttggcgccg gtgccgcctc gggtacgatg ccgctcctcc tcggagtgct
     8641 cgtctcgatc gtcttcttca cgttcttcta cgataccgtg gcggtctttg aggagacgaa
     8701 cgtctttgag tcgatccggc ggagcgtcga gttcgtcatg aacaacctgg gcaacgtcct
     8761 cgtcttctac ctggtcaaca tcgtcgtcct cgtgggcatc gggtttgccg ccttcgtcgc
     8821 catcgctgcc ctgctcgtcg acagactcga gccgctcacc cgcctggacc ctgccgaact
     8881 ccaggcggtg acgccgcagg atctcctcgg gctcatcggg accgagggta tatggaccat
     8941 cgcggccgtc tacgcagtcg tgatcgtgct cttcgcggcc ttcctttacg cctacaaggc
     9001 aagtttcttc cgagaccatg ccgcaagcgt ccccatacag cagggagagt acgacgagaa
     9061 gggccggtgg tataagtact gaggcggtgc cccggggcac cgattctctc tctttttacg
     9121 cgggggttca aacgggcggg ccgccggctc ccggagagtt ccggatcttt cgggcgagat
     9181 cccggcctct ggcgctcttc cccgatcttt ctgggaagag tggccggata cggaagcacc
     9241 ggaacgtgag tgcaggagag aggggatgcg cgagctcgat gccgcagatc gccgcgcctc
     9301 acgtcacgag gtagaccagc atcccaaaga ccccgccaaa gaacgtggcg gcgaggttcg
     9361 tgcccgagtt ccctatcctg ccgctgttct cgagcgttgc gcccacaagg ctgtcgacgt
     9421 tcgtgccgac aaaaccggcg gcgaccgtga cgacggccat gcgggggtcg gcgacgccca
     9481 gcatccaggc gacgagggcg acgagggcgg cggcggcgac ggccgccatc tcgccccgca
     9541 gggtcacccc cccgttcgtt ccccggggca ccggctggag cgtcgtgatc aggtagggcg
     9601 tcttccccgt gaccccgatc tcgcttgctg cggtatcggc ggcggcggag gcgacgctcc
     9661 ccatgaagag cgctgcaaac gcagggttcc cggtaacgcc gtagagtatg gcggcacccg
     9721 tcgccacgag gccgtttgca aagacgttga agtagccgcg cacgcccgcg tgctcctggg
     9781 cgaccccgag ctgctcttta tccccgtaac ggtaccgggt cgccccggcg ccgacgatga
     9841 agaaggtgag catgatcagg aaccacggca cgcccgcaaa gacgatgagg atgatcccga
     9901 tcatggcgcc cgagaagagg ccgctgacgt cggccacccg gagccggtag gacgaataac
     9961 cgaacccgaa cgctatcacg gccgcgacga cgaggatggt caggtcgacc tggaagttga
    10021 tctcctcaaa gaggaacatg gtcatcgcga cgccgagcgc ctcgaccatc aggacatcgt
    10081 cccggccgcg gagggccgcc cggagcagca ccgcggccac aacccccatc atgacgacga
    10141 gcggagcgct gtagcggagg tagagcatca ccccaagcga tacgccgacg gcggcagcca
    10201 ggtggtagag gtaggatgcc tgctttcctc ccgtgaaccg gaaggcgagc tcgccgatga
    10261 cgacgatgcc gagcgtgcag gtgaagacga acgtcgagag ccacccaaac ccgtagagcg
    10321 cggcgagcac gatgatcgag agcgagacgt accgggtatc ccggatgagg aagaggacga
    10381 gcgagaacgg tatgaggagg agggtgagca gccacgccgg ctggagcagg ggagcaaggc
    10441 ctatgaaggc gagggtgagc accgatgcca gaaccaaccc cggcggcttt gtcattcata
    10501 cacctttggc ggtgcaggga aaaagccttt agggtcgagt atccgccctc ttccctctcc
    10561 cgggatgtga gagccctggt gcgcacggca ggcccgggga cgccaaacct ccgtcgaaca
    10621 atatatttca caggaacgag agagatcata cgctgaaaat gtcgccgtca catcaattac
    10681 ccaaaaatta cgatatcgaa gaagtggaga ggcgctggca ggatacctgg cgggatgagg
    10741 agaactactt cgatcccgat tcaaccaaac cccggttcat catcgatacg ccgccgccct
    10801 accccaccgg caacttccat atcgggaacg ccctaaactg gttctacatc gacttcgtcg
    10861 cgcggtacaa gcgtatgcgc ggcttcaacg tcatgttccc gcaggggtgg gactgccacg
    10921 gccttcccac cgaggtgaag gtcgaggaga cctacgggat aaccaaaaac gacgtacccc
    10981 gggagaggtt ccgggagatg tgccgcgacc tcacgctcgg gaacatcgag aagatgcggg
    11041 cgaccatgcg gcgcctcggg ttctccaccg actggagcca cgagtacgtc accatgctcc
    11101 cggagtacta caaaaagacc caggcgtcgt ttttacagat gctcaaagcc ggcgacatct
    11161 accagagcga gcatccggtg aacttctgca cccggtgcga gacggccatc gcgtttgccg
    11221 aggtcaacta cgctccccgc acgaccacgc tcaactactt cgactttgac ggcgtcgaga
    11281 tcgccaccac ccggcccgag ctcctcgcgg cctgcgtcgc ggtggcggtc caccccggcg
    11341 acgagcggta tcgcggcatg aagggagcaa cgctcgcggt ccccatattc gggcaccgcg
    11401 tgccggtcat cgaagatgtc gcggtcgacc cggagttcgg cagcggcgcg gtgatgatct
    11461 gtacgttcgg ggataagacg gacgtctact ggtggaaaca gcacagcctg gaccttcgga
    11521 aggcaatcga ccggcagggc gtcatgaccg ccgtcgcggg gcgctacgcc gggatgtcgt
    11581 cggatgcctg caggaaggca atccttgagg atatggagga ggcgggtatc ctgaagcgcc
    11641 gggaagagct cgagcagcgg gtgggaacct gctggcggtg caagacaccg atcgagatcc
    11701 tctcggagcg ccagtggttc gtccgggttc accagaagga gatcctcgag gcggccaaag
    11761 agatcacctg gctgccggag catatgttca cccggcttga gaactggacg gagtcgatgg
    11821 agtgggactg gtgcatatcc cggcagcgga tattcgcaac gcctatcccg gtctggttct
    11881 gcgacgcctg cggcggcacg atcctcccgg ccatggagga tctccccgtc gacccgaccg
    11941 ccgatatgcc gaaagccccc tgcccgcggt gcggcggcac ccggttcacc ggcgagaagg
    12001 atgtgctcga cacctggatg gactcgtcga tatcggtgct ccacgtcacc gggtgggacg
    12061 ggagcagcag accaccgctc ttcccggcgc aactccgccc ccaggggcac gacatcatac
    12121 ggacgtgggc gttctacacg atcctccggt cgaaagccct ggccggcagc catccctggg
    12181 agcagatcct catcaacggc atggtgctcg gggacgacgg gttcaagatg agcaagagcc
    12241 ggaacaacat catctccccc gaagaggtcg tcgggaagta cggggcggat gcgctccggc
    12301 agtggggtgc cgggggggct gcaatcggct cggatatcat gttcaactgg aacgatgtcg
    12361 tcgccgcatc gcggttccag accaagctct ggaacatatt caggttcgtc atggggcacc
    12421 tggagcgggg tgccgatccg gcggcgccgg tgacggagct catcgaccgg tggctcctcg
    12481 tccggctctc cgataccgtc gcggaggcca ccgccgcgat ggagggctac cagttcgacc
    12541 gggcgctcaa ggccgtccgg gagtttgcct ggaacaccct tgccgacgac tacatcgaga
    12601 tagccaaggg ccgcctgtat gcggaggagg gcggcagggc gagcgccgca agcgcgctcc
    12661 ggacgaccat ggatgtcctc tgccgcctcc ttgccccgat cataccgcac ttcgccgagg
    12721 agtgctacca tatcctgacc ggaggaagcg tgcataaaga ggcctggccc gactttacgt
    12781 acgccgacga cgaagcgcgg cgccacggcg atctcctggt gaagacggtc gccgaactcc
    12841 ggcgctacaa gcacgataag ggtatggcct taaacgcccc gctcggccac gtgacgatct
    12901 acgcgccgga gccggtcgac gataccggcg acgccggccg tgcgctcgcc gcagacgccc
    12961 actggcgcac cggtacaccc cggctcgaag aggtcgcgga cggcgtggac ttcaacatgg
    13021 cggtcatcgg cccggcgctc aggaagcagg cccgcgggtt catggaggcc gtccgggcgc
    13081 tcccgccgga cctgctcaag aacccgcccg cggccgtcac cgtcgacggc gaggaggttt
    13141 ttgtcccgcc cggctccttc acgccgaaga tcgcctacca ggtggcgggc gaagaggtgg
    13201 atgtcctcac gctcggggat gtcgtcgtga cgataggaca ttcgtcctga tctcgtcggc
    13261 gatgaaccgg gcgacatcct cttttgggag cagcgcgtcg acgacgacga accgcgcggg
    13321 gtcggacgcc gcaaggtcca ggtagttctc ctgcacccgc gcgaggatgg cggcgttctc
    13381 gaagtactct ctcttttttt cagtatcaag cctcgcaacg gcatcttcga tggggaggac
    13441 caggaggaac gtccggtcag gccggaccga ccacccatcg tggatccggc ggagccagag
    13501 gagcgggtcg gggaggatgc cggcgaggac gaccggctga taggcaaacc gggagtcgga
    13561 gtagcggtcg gatacgacga gtctcccctc atcgagcgcg ggccggatca ccccgtcgat
    13621 atgcgcggcg tggtcggcgc agaagagcag cgcctcggtg atcgggtcca tccgttcggc
    13681 aaccgcccgc cgcaccgact cgcccaccca ggtcgcgccg ggttcgcggg tgaagagggg
    13741 gtcaagatcg gcgatcagct cgcggaggcg ggcgatgagc gtgctcttgc cgctcccgtc
    13801 gatcccctcg atggtgatca gcacgggtgc cccctccgga cccactcgag cggcaccgtg
    13861 ccgcggtgga cggcggttgc tatgatcgct ccatcgaacc cggcgtcatc aaggagacgg
    13921 atgtcgtccg ttccggcgac gccgcccccg tagaggaggc ggccgggata acatgcccgc
    13981 atctcctcga ggtcttcccg gacaaggccg cgctgtgtcc ccacggcgcc gatgttgagg
    14041 atgatgcacc cctcaaacga gaactccgac gcatggcaaa gcatctcctc cggccggatg
    14101 ccccagggga gcacccggcc tcctttgacg tcgatgctga ggtagccggc ccggtatgcc
    14161 gcaaggtcct cgatcgcttt tgccgccgtc tcggtgccga cgaccggcgt caccccggcg
    14221 accgccgtgc agtcggcagg cgaccggatg ccccggtcga ggtagcagcg ctcgacaagg
    14281 gcggtgcaac gccggacgag ttcgtcctgc ggggttctgc cctggatgct ctcgaggtcc
    14341 gcgatgtaga tgtaccgggg ttcgagggcg gcgatgtagg tctccggctc ggccgacggg
    14401 gcaagtcccc acgtcagggg gcggtagccg gcgcggtcgc ccgatcttcc gtgcacgaca
    14461 agaccgcccg ccagatcgac tgcaagaatc agttccatgt cctcaacgca atcactatta
    14521 gggtgaatga tcattaattt ctatgcaatt actcgtcagt ccgagcagca ttgaggaggc
    14581 aagaagctcg ctttccgctg acatcatcga tgtaaagaaa ccctccgagg gctcccttgg
    14641 ggcgaatttc ccctggatca tccgggagat caagaccctt gccggcagga agcctgtcag
    14701 tgctgcaatc ggggacaatg agtataagcc aggaaccgcg gctctctcgg cctacggcgc
    14761 cgcccacgcg ggtgctgatt ttatcaaggt cggcctgatg ttcgacggca ccgaccatgc
    14821 ccgcgaggtc atcggggcgg tgaccacggc ggtgaaagag gagtttcccc ataaatacgt
    14881 cgtcatcgcc gcttacgccg attttcagag gatggacacg atctctccgc tcgcgatcag
    14941 cccgctggtg gcggatgccg gggcggatat cgccatgatc gataccggca tcaaagacgg
    15001 gaagagtctc tttgagttca tggacgagga agcgctgacc cggttcaccg aacagaaccg
    15061 ggggttcgga ctgaagacgg cccttgccgg ttcgctgagg ttcgaggatc tggatgcctt
    15121 gaaacgcatc gatcccgaga tcatcggcgt ccgggggatg gtctgcgggg gcgatcgatc
    15181 cgccacgatc agggccgagc tggtcgagaa agcaatgatg atgctcaggt gatgtatgta
    15241 tatcgagaag atgcagatgg agacaacgtt tacgttcgac gacgtgctgc ttgaacccgc
    15301 cgaatcctgg gtcgagcccg acgaggccga cgtcaggtcg cggttctcgc gcaacatccc
    15361 cctgtatatc cctctcgtga gcgccgccat ggatacggtg accgagtcgg tgatggcgat
    15421 aacgatggcc cgggaaggcg gcatcggggt catccaccgg aatatgccgc cggaacgcga
    15481 ggtggccgag atccggatcg tcaaacaggc cgaagacctc atcgagcgcg aggtcgtggc
    15541 ggtcggcccg gactccacgg tcgccgatgt ggaacgggtc atgaagctgc acggtatcgg
    15601 gggcgtgccc atcatcgagg ggagcaaggt gatcggtatc gtcagccgca gggacatcag
    15661 ggcgatcctg ccccggcgcg gcgaggcgaa gatcacggag tacatgaccc ggaaactgat
    15721 cacggcgtct gaagacgtca cggccgagag cgcgcttgag acgatgtacg caaacaaggt
    15781 cgaacgcctt ccggtcgtgg acggcgaggg gtgtctggtc ggcatcataa cgatgcgcga
    15841 cattctcgaa aaacggcagt acccccgcgc gaaccgcgac gcaaacggga aactcagggt
    15901 cgccgccgcg gtggggcctt tcgacttcaa ccgggccatg ctgctcgtcg atgggggcgt
    15961 cgacgccctg gtggtggact gcgctcacgg ccataacatg aacgtcgtca acgcggtcag
    16021 ggagatcaag gcaagcgtcg ccatcgatgt cgtggcagga aacatagcga cgaggcaggc
    16081 agcaggagcc ctggtcgact ccgtcgacgg gctgaaggtg ggcatcgggc cgggttccat
    16141 ctgcacgacg aggatcgtcg cgggcgtggg cgtgccgcag gtctccgcga tagccagcgt
    16201 cgccgaggtg gcgcgcgatg ccggcgtgcc ggtgatcgcc gacgggggca tccgctactc
    16261 cggcgacatc gcgaaggcaa tcgccgccgg cgcggacagt atcatggcag gcagcctctt
    16321 tgccggaacc gacgaagctc cggggaggat tacgacgatc aagggccggc gctacaagca
    16381 gtaccggggc atgggatcgc tcggcgtcat gagcggcggg gaatcaagcg accgctactt
    16441 ccagaagaag gagatcggga agaccaagtt cgtccccgag ggtgtcgagg gggtgacccc
    16501 ctacgtcggg agggtctcgg atgtcatcta ccagctcgtc ggcggcctga agtccgcgat
    16561 gggctacacg gggtcaaaga cgataggaga cctgaagaag aacggtaagt tcctcaggat
    16621 cacgtccgcc ggctatggcg agagccaccc gcacaacatc atgatcaccg acgaggcgcc
    16681 gaactaccgc ctcctcgatt aaccttttat attaaaaaca ctaacgatca gtaaacatgc
    16741 tcgagggaga tgaggtttcc cgtctcctcg atatcctggg aaaccggaac aggcggcgaa
    16801 taatagaact gctgaggcag aaaccgtgtt ttgtgaccga gatctccgag cgcctggtgc
    16861 tcagtccgaa agcggtgata gagcacctgc agatgatgga gcgcgaggag ctcctcgtct
    16921 cgtgccagga cgagcgcaga cgaaagtatt actacctctc ccatgacatc aatgtcatcg
    16981 taaacctgca gaagcaggat gggattacac ctccctccgt tgaagaggac cagagggcac
    17041 ggtttacgag gaacctctcg gcgctcagga ggatgatcct ggcccgcgac gagctcctgg
    17101 agaacctcga acaattggag cgggagatcg agtcgaggtt cgtcgaggtc gtacgcacag
    17161 ggggagggtt caccgctgac gatggggacc tggagatcat ccttgccctc tcccacgctg
    17221 ctctgacgct tgcggaactc gaagaagtga gcagcctgtc cacggatgag ctgaaacaga
    17281 gactgggcag cctcatgcac atggggattg tcaggcggat caacgatcga tacaggatac
    17341 gtggtacata tggcgaataa tccatacgac gagatgctga agaacatcgc gcagctgatg
    17401 gggaagatcc tgagcgagat gcctctccag gacccgaaga tcatcgggtt caccatcatc
    17461 agcggggtcc ccgagggagc gccctacctc gacctggccg gggatgaagg tgccgggttt
    17521 gaggtgatcg agggggacga ctgcatctac atcaccgcaa cggtgaatgc gcaggtccag
    17581 ggcgctccct acgtcacgtt ccagagggag tccgtgaccc tctgcaccgg gggcgatgag
    17641 gagacgatca tcggcctcaa ctgcgagatc gatataccgc acagtttcta caacgtgcag
    17701 cacggcgtca tcgatgccgt ctgccggaag aggagcaccc cgggcgaggg agcccggccc
    17761 tgaaccgggt gcgcatcccg ccctccttct ctttttaca
//
LOCUS       DAOSII010000029        17793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_3393_length_17793_cov_44.074417, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000029 DAOSII010000000
VERSION     DAOSII010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 17793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17793
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3393_length_17793_cov_44.074417"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..1474
                     /locus_tag="PLV90_03670"
     CDS             176..1474
                     /locus_tag="PLV90_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOF96242.1"
                     /translation="MSKTLVVGITLLLLCIGISAVSAEIGGDQGWYRIHCNVDGASVY
                     FDGEYKGTISGGELYVAVYTTGTPYSTIRVERSDYRTYTASLPKAPIMGDTAEVYATL
                     QPAETYGSIYVSSSPSGAAIYLNGNYRGVAPLTIRDLTPGTYNVEADLSGYQSDWSRV
                     TVQSGKQSNVQFTLTPIQQYGSVSITSSPSGAYVYMDGVYKGRTPLTLNNVAANNHNV
                     ELDLSGYYDWRTTVSVASGVTRYVDARMVAIPSETTGRIDVASSPTGADVYLDGVHQG
                     KTTSTGPFTISNVRVGTHTVRVAMSGYHDYTTSVDVGGATTSYISAALQPASMSATGS
                     IAVASSPSGAEIYIDNAYRGITPLTVDGISAGTHTVMVALNGYNDWSTSVQVGAGSIA
                     SASASLSPVSAPTAKAGVAPFAAIGALAALGLLGARRKQN"
     gene            1624..3132
                     /locus_tag="PLV90_03675"
     CDS             1624..3132
                     /locus_tag="PLV90_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HOF96243.1"
                     /translation="MEKSLDLINARIRDGSARVVTAEEMPAIVDALGEEGALEEVDVV
                     TTGTFGAMCSSGAFFNFGHADPPIRMERVWLNDVEAYAGIAAVDAYLGATQEADTQDR
                     VYGGAHVLEELVAGGTVELRATSHGTDCYPRRSITTDLLLEDMNQAIMLNPRNSYQRY
                     NAATNTTDRTLSTYMGTLLPRCGNVSYSGAGTLSPLANDPNFRVIGSGVPIFLAGAEG
                     MVVGEGTQHSSGGGFGTLMVTGDMKRMRQEFLRAAVMPGYGVTIYVGVGVPIPVLDTG
                     IVRSTAVRDEDILTDVIDYGTPRRDRPSMAKVSYADLRSGSIDIGGEAVRTSSLSSYR
                     RAREVARELKTWVEQGKMELTLPVRRLDPAKRAKPMRETAITPRVRDIMNRQVISIAE
                     DEDIRAAAKRLLRGETNHLPVLAGDGTLVGIITTYDVSKAVVTDGRLRQVKDIMTRQV
                     IKTTPDEPVDIAARKLEQNNISALPVVDATNRVVGILSAIDLGKLFGGRRWR"
     gene            3129..3527
                     /locus_tag="PLV90_03680"
     CDS             3129..3527
                     /locus_tag="PLV90_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOF96244.1"
                     /translation="MKLLLTYSRRGQSDPGRKPVIARIVKETGILVNVEKANIDSTAG
                     EVLIDIPDADADLVRRCLEEMGVAVRVMEDAVTLDEAECVDCGACISICPQEVFSFDD
                     EWRLRVSAARCVLCGKCIRACPHSALSLQG"
     gene            3524..4243
                     /locus_tag="PLV90_03685"
     CDS             3524..4243
                     /locus_tag="PLV90_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HOF96245.1"
                     /translation="MIREHFEFRQTIATILADEPRHVAAAKAGMLAARHEVERQIALD
                     PYFAATFEPYAPKDPERTVARMVRAAGEAGVGPMAAVAGAIARAGVEAMVEAGASFAL
                     IDNGGDIALVSDRDLTIGVYAGASSLSGRIAFRLSPQMDILGICTSSATVGPSISFGV
                     ADAVTVFAPDPAVADAWATAICNRITADDTSVLDALPGTGVRGVLAIIDDAVIRHGDL
                     PRIVRAKVDERLITAGRPDPR"
     gene            complement(4255..4647)
                     /locus_tag="PLV90_03690"
     CDS             complement(4255..4647)
                     /locus_tag="PLV90_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96246.1"
                     /translation="MADYMDLADIAERLFEASDDDDERLAGRLDTLDEETRRALLASD
                     LLNAYQVFYFWFRETPDDLAMERLQLHAASDLARGIVIDEIDIYDLVFSVKGGNPVLV
                     LTDGENTLARFEGERAYGEILEYLDEHP"
     gene            4775..5056
                     /locus_tag="PLV90_03695"
     CDS             4775..5056
                     /locus_tag="PLV90_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOF96247.1"
                     /translation="MREEDLDWTVYHCIPENSDITTPDLVAATGFEPGAVMASLERLE
                     HYLLIRRSGDGVRLLSLQESLIACQCRHTKDDLPFVIENGVVRATRRGD"
     gene            5053..5586
                     /locus_tag="PLV90_03700"
     CDS             5053..5586
                     /locus_tag="PLV90_03700"
                     /EC_number="2.1.1.206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(56)-2'-O)-methyltransferase"
                     /protein_id="HOF96248.1"
                     /translation="MSGAPTVAVLRVGHRPERDQRVTTHVGLAARALGARGMYLAADD
                     PGVITSITDVASRWGGDFFVENDVSWRRCIGDWKAGGGKVAHLTMYGLRMTDVIDDIR
                     REERVLVVVGAEKVPGDIYGLADYNVSVTTQPHSEISSLALFLDHLFEGRELNREYPD
                     AKIRIEPAKAGKKTVER"
     gene            5583..6659
                     /locus_tag="PLV90_03705"
     CDS             5583..6659
                     /locus_tag="PLV90_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOF96249.1"
                     /translation="MKGRVLVAGFATRHIAQSACRAGYTVYAVDHFCDRDLAWYTEDR
                     LSFDELADLPAKIAELAARHPVDALVVASGAEAVGTTIPLCGTPPAKVERFLDKLEIQ
                     RFFEGLDVPVPPLAGRDRYPAMVKPRRGAGGWRNAVAWAEEDLRRWEEAWPDVPYVAQ
                     QLVDGIPSSVSCIADGRRALAVAVNRQILRGEGECAHGFAGSVTPFSHPLAGEMVACA
                     ERIAAASGCTGSVGIDFMAGEKPWAIEINPRFQATLDTVEMATGENVFALHMDACRGV
                     LPATRPPARRVAVRRILFAERDMRLDADLSALAPRVADIPWPGTEFEEGQAVVSIFGC
                     AETEAAAFHDLERNIAAVRGLIGG"
     gene            6766..7287
                     /locus_tag="PLV90_03710"
     CDS             6766..7287
                     /locus_tag="PLV90_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HOF96250.1"
                     /translation="MVSITELLNDPAINAYVLRLIGEEGVELLKRFPEGGEHSDEELA
                     EKTGVNLNTVRHTLYTLYEKRLAEYRRLKNTETGWLTYLWHLRLDRLHGVIEEEIRDV
                     LEHLDARLTYEEKNDFYMCKDCGIIYTFTDAAGWNFECPNCEEMLEHFDNELIANALR
                     RRVDRIKESLGSA"
     gene            7280..7762
                     /locus_tag="PLV90_03715"
     CDS             7280..7762
                     /locus_tag="PLV90_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOF96251.1"
                     /translation="MREEDCIALLRRAGCSDDVVAHCRAVRDLALTYADGIADRDLIE
                     AGALLHDIGRGVTHDLHHAEAGGAICRAFGLDERIERIVERHIGAGLTADECSLLCLA
                     PRDCMPATLEEKIVAHADNLVKGTRAITMEERMMHAIALPRRQRKRIRRLGLDMELFR
                     "
     gene            complement(7755..8258)
                     /locus_tag="PLV90_03720"
     CDS             complement(7755..8258)
                     /locus_tag="PLV90_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator of amino acid metabolism, contains ACT
                     domain protein"
                     /protein_id="HOF96252.1"
                     /translation="MWADISREFADSPSQTRVVRFLLENGFGINEEGRIVCNGVEIPA
                     THIGRAIETDRRVVDATARRILDNPDLREVFIRMRASPDLSRVAEALGLSVITLYPKD
                     AQQKGIVGAAVKVLVDHDLSIRQIFVTDPYLAEEPRLVMIVDGRRVPASVYEDLRALP
                     QVKRLII"
     gene            8430..8514
                     /locus_tag="PLV90_03725"
     tRNA            8430..8514
                     /locus_tag="PLV90_03725"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8466..8468,aa:Leu,seq:gag)
     gene            complement(8826..9254)
                     /locus_tag="PLV90_03730"
     CDS             complement(8826..9254)
                     /locus_tag="PLV90_03730"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOF96253.1"
                     /translation="MESDSSLEHIRRIQALIGRIEELADRIDRYQQGSNVQLNALREE
                     CDAVLAEAKQDYHALIVSAEMRGQDVTPDIALEDRRLKNTWLALYRHHGGATAEEISG
                     ELKRHRTTVSTYLNTLVLMGYAKKERVGHEVHYTAILNKD"
     gene            9325..9513
                     /locus_tag="PLV90_03735"
     CDS             9325..9513
                     /locus_tag="PLV90_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96254.1"
                     /translation="MTGAEREDPPESAGQFTGIILFTLLKIILFAGTKICRRSLVKLD
                     ADPYLDGRYLPARKEGMR"
     gene            complement(9640..10227)
                     /locus_tag="PLV90_03740"
     CDS             complement(9640..10227)
                     /locus_tag="PLV90_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96255.1"
                     /translation="MKVTEDGEQADDSALSIVLVFFDVYQKSIEVTIRRTMEEFLSSL
                     QSELSRPFDLSTMHQHNPLISQFKHKKEGFCPILHEIDGSIKIGITEIGIWDPVPPSR
                     FLYGVECDAHASLLSLYRFRQESTETDQLLQRLRKATIKIFGVACGLYHCDDPGCFLT
                     YHMTISDLDTGMYACRGCRDDFIRALQSLNSRIGA"
     gene            complement(10899..11243)
                     /locus_tag="PLV90_03745"
     CDS             complement(10899..11243)
                     /locus_tag="PLV90_03745"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOF96256.1"
                     /translation="MQKYDFTGASNGADCLVRQAREKSMHGDHTAAVEYLMEAVDIEP
                     KHPEAFALMGDCCDYLGQYEQAIANYDRALELDPYHADAWFNKGMSLRSIGRNIEAVQ
                     CIEKSIELYCGR"
     gene            11573..14026
                     /locus_tag="PLV90_03750"
     CDS             11573..14026
                     /locus_tag="PLV90_03750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOF96257.1"
                     /translation="MSVLDQERMDQIKQHLKWNPRGMTISDVSSKMHMNRNLVAKYLD
                     MLLISGQIDMQEMGTAKVYFLSHRVPISAMLDFSSDMVVVLDDENRILRVNGPMLAVL
                     GEDRQSVVGTDLDDSQNPFLIALRAHLPPPESRGAPDQIIGVRCALQGKECHFRVKRI
                     PTVFEDGGRGITLIIEDVTDQVANRERLKVSEARYRGIIEDQTEFIVRFMPDGAATFL
                     NAAYARYLEESVEHLIGKPFLPRIHTEDAPIRDQAILLLNRDHPVSIFECRIRHPSGR
                     IRWNRWTVRALFDTSGHLSECQGVGRDITEKREAAAKVNDYIRGMEFLSQTCIAFMDM
                     HEDHNIYDYVARQICALAPGLLVWISTIDESDQNLVLKSVVGDPAAREVIGQLPGTHP
                     AGTTFPINQTDRAELVRCKSLVRMPPHSRLLQIQIPEELHSRIAKTTDSIDTYLMGLV
                     TKGRLIGSVGISQKSGSVLPSKDLIEAFIRQAAIAIDRRDASDTLKASLAREQEQVQN
                     LQFLSRTAMKFIEMEDSEDIYRYIADRLYDLVPESIIGINSFDSGRRELTLRAVAGRE
                     EQVDTFWKALGINNPLGTSFPISKLPRSEVDFCKNTLFEIPSVYDMFFHQIPQERCEQ
                     ARDKLQFGSAYCMGFSRKGEIFGNIFIGLFRPGTVTNREIIEAFINQASVALLRWFAR
                     EHHRESEGLFRVLVEQMPYPVVILGRDGYILGANGLAATLIGCRESAGLVGRPFRDCV
                     EGGADLPPGLSRCLVSYEGSDLSCRLCLQPPGKGACEVEAWGRMVRYNRKPALYVVLH
                     PLSAEMENGSIPSTMPSSP"
     gene            14409..14891
                     /locus_tag="PLV90_03755"
     CDS             14409..14891
                     /locus_tag="PLV90_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOF96258.1"
                     /translation="MACKCCQCGSCCSHMRIVHATIEERGNYTFIVHNAYNGDVKEVR
                     VDPDKIALFEDRSSIEELPDACPFLRFDEKTGKALCTVHLTRPDLCREYCCWRLLILD
                     PRGKRAGRVMYQTTFLPDDDELSRLWERVQPTLNGLCGTEWDGAVIDALTRAGYRVRR
                     "
     gene            15259..16542
                     /locus_tag="PLV90_03760"
     CDS             15259..16542
                     /locus_tag="PLV90_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOF96259.1"
                     /translation="MDIKTERRDGILTFAIRGRLDGYGAGQLADAVEASLQADDRSVV
                     FDLAGMSYLSSAGIRILIATKKRLKERGGTLALAGIQDYPKSVLDMAGVTPIFSLYPD
                     LEVAVAACRRSKDSLSVINDLVRAPVVRDGVAYTVEPASTRSSSLLVTGDLDKMLHAR
                     LTESDIRAIPFSALPYSLGLGALGENVADALPLLGEMITLHGSVVWLPTDGNATPDFF
                     TPVKDAGKPCIYTGYNVTLYGPFHEIITIDAGDEGGIALSDIYRTIFDFARERKRDFS
                     GVVALAMWAVVDGVCSLGVKRSPIAASSPANGLPINAEENAKEWLEENDEPKYRGETM
                     VSFGFGIDYSACSGEHLSALTDRNRPKTQQLQLHNHGVIFRGVPWDASLDLTRQIERI
                     VTGAEFVDMRHLKDNTRIRRAKVGVAYISEIAVEE"
     gene            complement(16610..17083)
                     /locus_tag="PLV90_03765"
     CDS             complement(16610..17083)
                     /locus_tag="PLV90_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOF96260.1"
                     /translation="MAEKSRPPLPPFTYETAVQKVRMAEDAWNSRDPEKVSLAYSIDS
                     IWRNRSEFINGREEIVAFLQRKWAKELDYRLIKEVWAFHEARIAVRFAYEWHDDSNNW
                     FRSYGNENWEFDESGLMRRRYASINDLPIKEGERKYHWPLGRRPDDHPGLSDLGL"
     gene            17504..>17793
                     /locus_tag="PLV90_03770"
     CDS             17504..>17793
                     /locus_tag="PLV90_03770"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOF96261.1"
                     /translation="MKQVLLVTDPGVRAAGWADAVGESLTEAGVPFTIFDAISPNPRS
                     GQVMAGAEVYHSEECNGIVAVGGGSPMDCAKGIGIVSSNMQDILTFEGVDRVP"
BASE COUNT         3599 a         5357 c         5398 g         3439 t
ORIGIN      
        1 tgccggcaga gagcatatgg gtagccggcc ggcaagtaac ccggggtgtc gggtccatcg
       61 agcggagaaa attctgcagt tttgctttaa gtctatggag atagagtata tcgtatagaa
      121 ataattatcc gtgactaaac cgacctacca ctcattgttt atcggagaac cgattatgtc
      181 aaagactctt gttgtcggaa taacgcttct tctcctctgc atcggcatct ctgccgtatc
      241 cgcagagatc ggaggagacc agggctggta tcgcatccac tgcaacgtcg acggcgcctc
      301 cgtctacttt gacggagagt acaaaggcac catatccgga ggcgaacttt acgtcgcggt
      361 ctacaccacc ggaacgccct acagcaccat ccgggttgag aggagcgatt atcggaccta
      421 caccgccagc ctgccgaaag cccccatcat gggcgatacc gcggaggtct acgcaaccct
      481 gcagccggcc gagacctacg gctcgatcta cgtctcgtcg agcccctccg gtgcggcgat
      541 ctacttaaac ggcaactacc ggggcgttgc gccgctcacg atcagggacc tcacccccgg
      601 cacctacaac gtcgaggccg acctctcggg ctaccagtcc gactggtcca gggtcacggt
      661 ccagtcggga aagcagtcga acgtccagtt caccctgacg cccatccagc agtacgggtc
      721 ggtcagcatc acctcaagcc cctcgggcgc ctacgtctac atggacgggg tctacaaggg
      781 ccgcaccccc ctgacgctca acaacgtcgc ggcgaacaac cacaacgtcg agctcgacct
      841 ctccggctac tatgactgga ggacgaccgt ctccgtcgcg tccggcgtga cccggtacgt
      901 cgatgcccgt atggtcgcca tcccctccga gaccaccggg cgcatcgacg tcgcctcctc
      961 tccgacgggc gcggacgtct atctcgacgg cgtccaccag gggaagacga catcgacggg
     1021 accgttcacg atatcgaacg tccgtgtcgg gacccatacc gtgagggtcg ccatgtccgg
     1081 gtaccacgac tacaccacat cggtcgacgt cgggggagcg acgacgagtt acatcagcgc
     1141 ggccctccag cccgcctcga tgagcgcgac cggctccatc gcggtcgcct cctctccatc
     1201 gggcgccgag atctacatcg ataacgccta caggggcatc accccgctga ccgtcgacgg
     1261 catatccgcc ggcacccata cggtcatggt ggcgctcaac ggctacaacg actggtcgac
     1321 cagcgtccag gtcggcgccg ggagcatcgc atcggcctcg gcatccctct cgccggtgtc
     1381 ggccccgacg gcgaaggcgg gcgtggcgcc ctttgccgca atcggtgcgc tcgccgccct
     1441 cggactcctc ggagcccggc gaaaacagaa ctaaaacact ctcttttttt attatccggg
     1501 cgcgaggtgc gtccctcccg ggaccgtgaa ggaaaccgga gccggctatc cccgtgccgg
     1561 agcaccgtat taccgaaagt aatagtcaac acctataaat attccagaat agatatacgt
     1621 ttcatggaaa agtcccttga cctcatcaat gcccggatac gcgacggtag tgcacgagtt
     1681 gttaccgccg aagagatgcc ggccattgtc gacgcactcg gagaggaagg ggcactggag
     1741 gaggtcgacg tcgtcacgac cgggacgttc ggggcaatgt gctcctccgg cgcgttcttc
     1801 aacttcgggc acgccgaccc ccccatcagg atggagcgcg tctggctcaa cgacgtggag
     1861 gcctacgccg gtatagcggc ggtcgacgcc tacctcggcg caacccagga ggccgacacc
     1921 caggaccggg tctacggcgg cgcccacgtc ctcgaagaac tcgtcgccgg gggcaccgtc
     1981 gaactgcggg cgacatccca cgggaccgac tgctacccgc gccggagcat cacgacggac
     2041 ctcctcctcg aggacatgaa ccaggcgatc atgctcaacc cgcgaaactc ctaccagcgc
     2101 tacaacgcgg cgacgaacac caccgaccgg acgctctcca cctacatggg cacccttctc
     2161 ccgcggtgcg gcaacgtctc ctactccggc gccgggaccc tctcgccgct cgcaaacgac
     2221 cccaacttcc gggtcatcgg gagcggcgtc ccgatattcc ttgccggagc ggaggggatg
     2281 gtcgtcggcg aaggcaccca gcactcttcg ggcggcggat tcgggacgct gatggtgacc
     2341 ggggatatga aacggatgcg ccaggagttc ctgcgggcgg cggtgatgcc cggctacggc
     2401 gtgacgatct acgtcggcgt cggcgtcccc ataccggtgc tcgataccgg catcgtccgg
     2461 agcaccgcgg tgcgggacga ggatatcctg accgacgtca tcgactacgg caccccccgg
     2521 cgggaccgcc cctcgatggc gaaggtcagt tacgccgacc tcaggagcgg ttctatcgat
     2581 atcggcgggg aggcggtcag gacgtcgtcg ctctccagtt accggcgggc acgggaggtg
     2641 gcgcgggagc tcaaaacctg ggtcgagcag gggaagatgg agcttacgct ccccgtccgg
     2701 cggctcgacc cggcaaaacg cgcaaaaccc atgcgggaga cggccatcac cccccgcgtc
     2761 cgcgacatca tgaaccgcca ggtgatcagc atcgcggagg acgaagatat ccgggcggcg
     2821 gcaaaacggc tcctccgggg cgagacgaac cacctcccgg tcctcgccgg cgacggcacg
     2881 ctcgtcggga tcatcaccac ctacgacgtc tcgaaggcgg tcgtgaccga cgggcggctg
     2941 cggcaggtca aagacatcat gacccggcag gtgatcaaga cgacccccga tgagccggtg
     3001 gatatcgccg cccggaaact ggagcagaac aacataagcg ccctcccggt ggtggacgcg
     3061 acgaaccggg tggtcggcat cctctcggcg atcgatctcg gcaaactctt cggcgggagg
     3121 cgatggcgat gaaactcctg ctgacgtact cgcggcgggg gcagagcgac cccgggagga
     3181 aaccggtgat cgcccggata gtgaaagaga ccggcatcct cgtcaacgtc gagaaggcaa
     3241 acatcgactc gacggccggc gaggtgctga tcgatatccc cgatgcggat gcggacctcg
     3301 tccggcggtg cctcgaggag atgggggtcg cggtccgggt gatggaggat gccgtcaccc
     3361 tcgacgaggc ggagtgcgtc gactgcggcg cctgcatcag catctgcccc caggaggtct
     3421 tctccttcga cgacgaatgg cgtctccggg tgagcgccgc gcggtgcgtc ctctgcggca
     3481 agtgcatccg ggcgtgcccc cactcggccc tctcgctcca gggatgatcc gggagcactt
     3541 cgagttccgg cagacgatcg cgacgatcct cgccgacgaa ccccggcacg tcgcggcggc
     3601 aaaggccggc atgctcgccg cccggcacga ggtcgagcgg cagatcgccc tcgaccccta
     3661 ttttgcggcg acgttcgagc cgtatgcccc gaaggatccg gagagaaccg tcgcccggat
     3721 ggtgcgggcc gccggggagg ccggcgtcgg tcccatggcg gccgtggccg gcgccatcgc
     3781 ccgggcggga gtggaggcga tggtcgaggc cggcgcatcg ttcgccctca tcgacaacgg
     3841 gggggatatc gccctcgtga gcgaccgcga tctgacgatc ggcgtctatg caggcgcttc
     3901 atccttgagc gggcgtatag cgttccggct ttcgccacag atggatatcc tcggcatatg
     3961 cacgtcgtcg gcgacggtgg gcccgtcgat cagcttcggc gtcgccgacg ccgtgacggt
     4021 ctttgccccc gacccggccg ttgcggatgc ctgggcgacg gcgatctgca accggatcac
     4081 ggcggacgac acctccgtcc tcgacgccct tccggggacg ggtgtccggg gggtgcttgc
     4141 gatcatcgat gacgccgtta tccgccatgg ggacctcccg cggatcgtcc gggcgaaggt
     4201 cgacgaacgg ctgatcaccg ccggaagacc ggacccccgt tgaggatcat tgcactacgg
     4261 gtgctcgtcc aggtactcga ggatctcccc gtacgcacgc tccccctcga accgcgcgag
     4321 cgtattctcc ccgtcggtaa ggacgaggac ggggttcccg ccttttacgg agaagaccag
     4381 atcgtagatg tcgatctcgt cgatgacgat cccccgggcg aggtccgacg ccgcatggag
     4441 ctgcagccgc tccatcgcaa ggtcgtcggg cgtctcccgg aaccagaagt aaaagacctg
     4501 gtaggcgttg aggaggtcgg aggcaagcag cgctcgccgg gtctcttcgt cgagcgtatc
     4561 gagcctcccc gcgaggcgtt cgtcgtcgtc gtcgctcgcc tcaaagagcc tctcggcgat
     4621 atccgcaaga tccatataat cggccatacg tcgtttcacc ctgccccgcg cccctttcct
     4681 cccggagaac cgccctggca gcggccctcc cggccggaac ggtgcgggcg gcgcatgaat
     4741 aagtatatcg gtccgcccgg aaaaaagaat aggtgtgcgt gaggaagatc tggactggac
     4801 cgtataccac tgtattcccg aaaacagcga cattacgacc ccggacctgg tagcagccac
     4861 cgggtttgaa cccggtgcgg tcatggcatc gcttgagcgc ctcgagcact atctcctgat
     4921 ccggcggtcc ggcgatgggg tacggcttct ctctctccag gagtcgctga tcgcgtgcca
     4981 gtgccgccat acgaaggatg atctcccgtt cgtcatcgag aacggcgtcg tcagggcaac
     5041 gaggagaggg gattgagcgg cgcgcccacc gtagcggtgc tccgggtagg ccaccggccc
     5101 gaacgcgatc agcgggtgac gacccacgtc ggactcgcgg cgcgggcgct cggcgcccgg
     5161 ggcatgtatc tcgcggcgga cgaccccggt gtcataacga gcataacgga cgtggcctcc
     5221 cggtggggag gagacttctt cgtggagaac gacgtctcgt ggcgccggtg cataggggac
     5281 tggaaggccg gcggcggcaa ggtcgcccac ctcacgatgt acgggctccg catgaccgac
     5341 gtcatcgacg acatccggcg cgaagaacgg gtgctcgtcg ttgtgggggc ggagaaggtc
     5401 ccgggcgaca tatacggcct tgccgactac aacgtctcgg tgacgaccca gccccactcg
     5461 gagatatcga gcctcgccct cttcctcgac cacctcttcg aggggcggga gctcaaccgg
     5521 gagtacccgg acgcgaagat ccggatcgaa ccggcgaaag caggcaaaaa gacggtggag
     5581 cggtgaaggg gcgggtgctg gttgccgggt ttgccacgcg gcacatagcg cagtcggcct
     5641 gccgggccgg ctacacggtc tacgccgtcg accatttctg cgaccgggac ctcgcctggt
     5701 acacggagga ccgcctctcg ttcgacgaac tcgccgacct cccggcaaag atagcggaac
     5761 ttgccgcccg ccacccggtc gacgcgctcg tcgtcgcatc gggcgccgag gccgtcggga
     5821 cgacgatacc gctctgcggg acgccgccgg cgaaggtgga gcggttcctc gacaaactcg
     5881 agatccagcg gttcttcgag gggctggacg ttccggtccc gccgcttgcg gggcgcgacc
     5941 gctacccggc gatggtcaaa ccgcgccggg gagcgggagg atggcgaaac gcagtcgcgt
     6001 gggcggagga agacctccgc cgatgggaag aggcctggcc ggatgtgccg tatgttgcac
     6061 agcaactcgt cgacgggata ccctcgagcg tctcctgcat cgcggacggc cggcgcgcgc
     6121 tggccgtggc ggtcaaccgg cagatcctcc ggggcgaggg cgagtgtgcg cacgggttcg
     6181 ccggatcggt caccccgttc tcccacccgc ttgccgggga gatggtcgcg tgcgcggagc
     6241 ggatagccgc cgccagcggg tgcacaggtt cggtcggcat cgacttcatg gccggggaga
     6301 aaccctgggc gatcgagatc aacccccggt tccaggccac cctggatacg gtcgagatgg
     6361 cgacggggga gaacgtcttt gccctgcaca tggacgcctg ccgcggggtt ctcccggcaa
     6421 cgaggcctcc ggcgcgaagg gtcgcggtcc ggcggatcct ctttgcggag cgcgacatgc
     6481 ggctcgatgc ggacctctcg gcgctcgcgc cgcgcgtcgc cgacattccc tggccgggaa
     6541 cggagtttga ggagggtcag gccgtcgtga gcatattcgg gtgcgcggag acggaggcgg
     6601 cggcatttca cgatctcgag cggaatattg cggccgtccg ggggctgatc ggggggtgag
     6661 ccgttcggga gaataggaaa agaagataag ggttgagtgt acaatacgat aggtatcgat
     6721 cccgagcgcc tattataaaa tatcatctcc gggggagaat gaaatatggt ttctatcacc
     6781 gaactcttaa atgatccggc aatcaacgct tatgttctac ggctgatcgg ggaggagggc
     6841 gtcgaacttc tcaagcggtt ccctgagggc ggggagcaca gcgacgagga actcgccgag
     6901 aagaccgggg tcaacctcaa caccgtccgc cacaccctct acaccctcta cgagaagcgt
     6961 ctcgccgagt accggcgcct gaagaacacc gagaccggct ggctcaccta cctctggcac
     7021 ctccgcctgg accgcctcca cggcgtgatc gaggaggaga tccgcgacgt gctcgaacac
     7081 ctcgatgcac ggctcaccta cgaggagaag aacgactttt acatgtgcaa agactgcggc
     7141 atcatctaca cgttcacgga tgcggcgggc tggaacttcg agtgtccgaa ctgcgaggag
     7201 atgcttgagc acttcgacaa cgaactcatc gcaaacgccc tccggcggag ggtcgaccgg
     7261 atcaaggaga gcctcgggag tgcgtgaaga ggactgcatc gccctcctca ggcgcgccgg
     7321 gtgctccgac gacgtcgtcg cgcattgccg tgcggtgcgc gaccttgcgc tcacctatgc
     7381 cgacggtatc gccgaccgcg acctcatcga ggccggcgcc ctcctccacg acatcggccg
     7441 gggagtgacg cacgacctcc atcacgccga ggcgggcggc gcaatatgcc gcgcgttcgg
     7501 ccttgacgag aggatcgaga gaatcgtcga gcggcacatc ggtgccgggc tgacggccga
     7561 cgagtgctcg ctcctctgcc ttgcaccgcg cgactgcatg cccgcaacgc ttgaagagaa
     7621 gatcgtcgcc catgccgaca acctggtgaa ggggacccgc gccataacga tggaggagcg
     7681 gatgatgcac gcgatcgccc tcccgcgccg gcagaggaag cggatccgcc ggctcgggct
     7741 ggatatggaa ctcttcagat gatcaaccgt tttacctgcg ggagcgcgcg caggtcttcg
     7801 tagaccgatg ccgggacccg gcgcccgtcg acgatcatca cgagtcttgg ttcctccgcg
     7861 aggtaggggt cggtgacgaa gatctggcgg atggagaggt cgtggtcgac gaggaccttg
     7921 accgccgcac cgacgatccc cttctgctgc gcgtcttttg gatagagcgt gatgaccgag
     7981 aggccgaggg cttcggcgac ccgggagagg tcgggcgagg cgcgcatacg gatgaagacc
     8041 tcccggagat ccgggttatc gaggatgcgc cgggccgtgg cgtccacgac cctcctgtcg
     8101 gtctcgatcg ccctgccgat gtgcgtggcg gggatctcga cgccgttgca gacgatgcgc
     8161 ccctcttcgt tgataccgaa cccgttctcg agcaggaacc ggaccaccct ggtctgggac
     8221 ggtgaatcgg caaactcccg tgatatatcg gcccacatgc gggagtgttg gaaccgggcg
     8281 cataaatata caccgaaggc gggagtgggg caccggcgga agggtatgcc ccgggcgccc
     8341 ggagagcaac ctatttccgg tcgcagtgcg ttgtaatgaa gagcaggacg gggattgccc
     8401 ggtgcaggac cggcgattcc cgaatgcgtg cgagggttgc caagccaggt caaaggcgat
     8461 aggttgaggg cctattctcg taggagttcg tgagttcgaa tctcacccct cgcatggtat
     8521 tttccgggtt atgggtaagt gaagaatacc gctcttgcaa atcggtttat cttatggggc
     8581 agagcgggaa gaccccggtc gagagcgcgg ggatgaaagc accgccgccc ttcccatatt
     8641 gtgcgttttt ctccaaacag ggggtatctg cgttgcgacg atcaatcgac cggactgtta
     8701 cactctgctg cagggcgatt cctgcccgaa cagagttatt cacgcctgtg gagatcgtgt
     8761 gagtcctctt cggggcgggt tcgatgatag ccgggaagcc gcgcccgaga gtgcgaagta
     8821 gttcctcaat ccttattcag gattgcagta taatgaacct cgtgccctac ccgttctttt
     8881 ttcgcgtagc ccatcaggac aagtgtgttc aggtatgttg agacggtcgt tctatggcgc
     8941 ttcaactcgc cggagatctc ttctgcggtt gctcccccgt ggtgccggta aagggcaagc
     9001 caggtgttct tcaatcgccg atcctcaagg gcaatatcgg gggttacatc ctgccccctc
     9061 atctccgctg atacgatcaa ggcgtgatag tcctgttttg cttcggcaag aacagcatcg
     9121 cactcctcgc gaagtgcgtt gagctggaca ttgcttccct gctggtatcg atcgattcgg
     9181 tcagccaact cctcaatgcg acctatcagc gcctgtatcc ttctgatgtg ctcgagtgat
     9241 gagtcggatt ccatagtatt caactactta tgttgagcat cagacttgaa tgtgttgaag
     9301 tgttgagttg tttaccgtgc aggggtgacg ggggcggagc gggaagaccc gcccgagagc
     9361 gcggggcagt tcaccgggat tatcctcttt actctcctta agatcatcct atttgccggt
     9421 acgaagattt gcaggcgctc cctggtgaaa ctcgatgcag atccctatct ggacggaaga
     9481 tacctccccg ccagaaagga gggtatgaga taacagacac cctggagtac gatcatcact
     9541 cttcgcatac tgccgcccga aattctggtt gtgaagcgcc cgggcggagt gcggtgaaag
     9601 cgtgcacgct tcgtcctgag aagaggacgt ggagtaattc tacgccccta tccggctatt
     9661 cagggactgc aatgcgcgga taaaatcatc tctacaccct ctgcaggcgt acataccagt
     9721 gtcaaggtcc gatatcgtca tgtggtatgt cagaaaacag ccgggatcat cacaatgata
     9781 aaggccgcac gccaccccaa agatcttgat agttgctttc ctcaggcgtt gcagcaactg
     9841 atctgtttct gtgctttcct gcctgaaccg gtagagggac agaagagagg catgggcatc
     9901 gcactcaaca ccgtacagga atcgtgaagg cggcaccgga tcccagatgc cgatctctgt
     9961 gatgcctatc ttgatggaac catcgatctc gtgcaagatc gggcagaacc cctctttctt
    10021 atgcttgaac tgggatatta acggattgtg ctgatgcatc gttgaaagat cgaaaggtct
    10081 cgaaagttca ctctgaagtg atgagagaaa ttcttccatg gtccttctga tcgtaacctc
    10141 tatggatttc tgatagacat cgaaaaatac cagaacgata gatagagcac tatcatcggc
    10201 ttgttctcca tcttcagtaa ctttcaagtt aagacctcac attctcccat cgtgtgaatt
    10261 acagtcgttt cccgcatttc actccatgaa ggccgatttt tccccgacga gcgggaaaaa
    10321 gaggctcaat tttgacatgt tcaacccgat cgccggtatc tccggaaaat catcgcaccg
    10381 catcaccaga cccgcagact cacgaacggt gtcacctgtg cggtacctgt ttcctgcttt
    10441 ccatatcacc aaccatcaaa agttcctgcc cggtaaccgc catgtgctta ttacccccat
    10501 aagtgatcaa cagttaaata atttgtgttg agataccaac ggttctcggc gaggaagtga
    10561 acgttcatcg cgcaaagatc accccggaac ctggaacggc aaagggtatg agggcaagag
    10621 agcacatcgg atattccaca accaattgtt caaagtacgg cagttctgct ccttaaccga
    10681 accggtggta tgacggtgca ttgatccgaa cagataggca cagaaatacc tatggcagca
    10741 cagagcggcg atatgtcggt ggaattgttc atcagttacc ctgcccttta acataacctc
    10801 aaaagaggga tgcatccctt ctccctcgat tgctttccgg accctttgtt gtggtggtgt
    10861 ttgtcgaaag ttgtgttgtg tggtaacttg ctgctgcctc agcggccgca atacagttcg
    10921 attgatttct caatgcactg cactgcttct atgttcctcc cgatcgatct caagctcatt
    10981 cccttgttga accatgcatc agcatgatag ggatcgagct cgagtgcccg atcatagttc
    11041 gcgattgcct gttcatactg accgaggtag tcgcagcaat ctcccatcag agcaaacgcc
    11101 tccgggtgtt tgggctcgat atcgactgcc tccatcagat attcaactgc agcagtatga
    11161 tctccatgca tcgacttttc ccgtgcctgc cgaaccaggc aatctgcccc gttcgaggcg
    11221 ccggtgaaat catatttctg catcgcttct ctctcccgtg gtcacctggc gcattgccag
    11281 ttgctttgtg actgaactaa attactaacg gccttataat gaaaatttgc acgataataa
    11341 cgccgtagtg gtctgcaatt gccttatgca acaggatctg ccatgcaaat tattatatac
    11401 ttatctttag gcgtaatcgt gcacccagag caaccgcaat aatcagctta ccacggggat
    11461 gacagcgtgt tgaatcagtg ccggaagaga gggcagctac cagaaacccg gttacccgtt
    11521 tcaaggcctg ccagataaaa aaatttacta tcccagcacc ctatcacctt gtatgagcgt
    11581 gctcgatcag gagaggatgg atcaaatcaa acaacacctg aaatggaatc cgaggggcat
    11641 gaccatctcg gacgtttctt ctaaaatgca catgaaccgg aacctcgtag caaaatatct
    11701 tgatatgctc ctcatctccg ggcagatcga tatgcaggag atggggactg cgaaggtata
    11761 cttcctctcc catcgcgttc ccatctctgc gatgcttgac ttttcctcag atatggtcgt
    11821 cgttctcgat gatgagaaca gaatattgag ggtcaatgga ccgatgctcg cagtgctggg
    11881 cgaggatcgg caatctgtcg tcggtacaga tctggacgac tcacaaaatc cctttctcat
    11941 cgccctgcgg gctcatctgc ctccgccgga gtcgcgcggc gcaccggacc agatcattgg
    12001 ggtgcgttgc gccctgcagg gaaaagagtg tcacttccgc gtgaaacgga taccgacggt
    12061 atttgaagac ggaggccggg gcatcacgct gatcatcgag gatgtcaccg atcaggttgc
    12121 caatcgggaa aggctgaagg tgagtgaagc ccggtaccgg ggtatcatcg aagaccagac
    12181 cgagttcatc gtccggttta tgccggacgg cgctgctacg tttctgaatg ctgcatatgc
    12241 ccggtatctt gaggagagcg tggagcacct tatcgggaaa ccctttcttc cccgtatcca
    12301 taccgaagat gctcctatca gagatcaagc aattctatta ctgaatcgtg accatcctgt
    12361 ctcgatattc gaatgccgga tccgtcatcc ttcagggcgg attcgctgga atagatggac
    12421 ggtacgcgcg ctctttgata cgtccgggca cctctccgag tgccagggcg tgggcaggga
    12481 tatcaccgag aaacgcgaag ctgcagcgaa ggtcaacgat tatatccggg ggatggagtt
    12541 cctctcacag acctgtattg ccttcatgga catgcacgaa gatcataaca tctacgatta
    12601 cgttgcacga cagatctgtg cgcttgcccc cgggctactg gtatggatca gtaccatcga
    12661 tgaatccgac cagaatctcg tccttaaaag cgttgtcggg gatccggccg cacgggaggt
    12721 tatcgggcaa ctcccgggaa cgcaccctgc ggggacgacg tttcctatta accagacaga
    12781 tagggcggaa cttgtccggt gtaaaagcct cgtcaggatg cctccacact cccgacttct
    12841 gcagatacag atccctgaag agctccacag ccggattgcg aagacaacag acagcatcga
    12901 cacctacctg atggggctcg tcaccaaggg caggctcatc ggaagcgtcg ggataagtca
    12961 gaagagcggt tctgtactgc cgagtaagga tctcatcgag gcgttcatcc ggcaggctgc
    13021 gattgcgatc gataggaggg atgccagcga taccctcaaa gcgagcctgg cgcgcgaaca
    13081 ggagcaggtt cagaacctgc agttcctctc ccggactgcg atgaaattca tcgagatgga
    13141 ggactcggag gatatctatc gctatattgc ggatcgcctc tacgatcttg tcccggagag
    13201 tatcatcggc atcaattcgt tcgattcggg gcggagggaa cttacgcttc gagctgttgc
    13261 gggaagagaa gagcaggtcg ataccttctg gaaggctctc gggattaaca atccgctcgg
    13321 gacgtcgttt cccatcagta aactgccccg atctgaggtt gacttctgta aaaacacgct
    13381 ctttgagatc ccatcggttt acgatatgtt cttccatcaa atcccgcagg agcgctgcga
    13441 gcaggcccgg gataagttac aattcggcag cgcttattgc atgggatttt cccgtaaggg
    13501 tgaaattttt ggtaacatct ttatcgggct cttccggccg ggcactgtta cgaaccggga
    13561 gatcatcgag gcatttatca atcaggcctc tgtcgccctc ctgcggtggt ttgcgagaga
    13621 acaccaccgc gagagtgaag gactctttcg tgtgcttgtt gaacagatgc cgtaccctgt
    13681 cgtgattctc ggccgggacg gatacattct tggtgcaaac gggcttgctg ccactcttat
    13741 cggatgccgt gaatcagcag ggcttgtcgg gaggccgttt cgagactgcg tggaaggtgg
    13801 agcggacctt cccccgggat tgtcacggtg cctcgtttcc tacgaaggca gcgatctgtc
    13861 ctgccgacta tgtctgcaac ctcccgggaa aggagcctgt gaagtagagg cctggggacg
    13921 gatggttcga tataacagga aacctgcact gtatgtggtt ttgcatccgc tctcagcaga
    13981 aatggagaac gggagcatcc cgtctactat gccgagcagc ccttagtcta cagacgtgtc
    14041 aagacctcgt taccatgcct cccgcatcgc catctcctcg accttccgcg gtacgcaggc
    14101 agagcgggat cacctggcac gtttcgtctc tccgcgctgc cgggcggccg gcaaccgtcg
    14161 ccgccccccc gctctgcatt gagggccgtg cctctcctcc cgggcggtca agattcttct
    14221 gcgccgtgac cggcactgct acgccgagag ccgggcggcc ggcaaccgtc gccggcggct
    14281 ccggaaccgc ccggttgcag gtgtggtccg ggcggttttc cggcaggtat ttcatcattg
    14341 cgatgcgtag taccgggtaa tgagcatgcc gatacaccaa ccgcccggtc tttggggagg
    14401 cggtccccgt ggcgtgtaag tgctgtcagt gcggcagctg ctgcagccac atgcggatcg
    14461 ttcacgcgac tatcgaagag cgtggcaact acaccttcat cgtgcacaac gcctataacg
    14521 gggatgtgaa ggaggtgcgg gtcgacccgg acaagatcgc cctcttcgag gacaggagca
    14581 gtatcgagga gctgccggat gcatgccctt ttctgcgatt cgacgaaaag acgggaaagg
    14641 cgttgtgcac ggttcacctg acacgccccg acctctgccg cgagtactgc tgctggcgcc
    14701 tcctcatcct cgacccgcgg ggaaaacggg ccggacgtgt gatgtatcag acaacgttcc
    14761 tcccggacga tgacgaactc agccggctct gggagcgggt gcagccgacg ttgaacgggc
    14821 tctgcggcac ggaatgggat ggcgccgtca tcgatgctct cacgagagcc ggctaccgtg
    14881 ttcgccggta gctgccggga gactccgaaa aagaggggta tgcccctgtt cctctccgga
    14941 acgggctccc ctcggcccca tcgtatcgtc gagcccggcg gcaaccgatt gcgtgaacgc
    15001 cccaacccgg caccgtagca catcgcccgt ccccgttcgc agacgaggtg gtcggagggc
    15061 gtctttcctt cggttgcaac cggggggcaa tcccgacggt aaacgccgga gaactcaaga
    15121 gcgcggttat gtgagatccc cggtgccggg ccgttccggc gatccccgac aaagtcttcc
    15181 ccggcacgac acgccgatgg gggaacgaat ttgagaaaga ttaactgaca acagcctgat
    15241 atctataccg ggatacacat ggacatcaag accgagagga gagacgggat cctgacgttt
    15301 gcgatccgcg gaaggctcga cggctacggc gcagggcagc ttgcagatgc cgtggaggct
    15361 tccctgcagg ccgacgaccg gtcggtcgtc ttcgaccttg ccggcatgag ttacctgagc
    15421 agtgcaggca tccggattct catcgccaca aagaagcgcc tgaaggaacg aggcggcacg
    15481 ctggccctcg cgggaataca ggattaccca aaaagcgtcc tcgatatggc cggcgtgacg
    15541 ccgatcttct ctctctaccc ggatctcgag gtggccgtcg ccgcctgcag gcggtcaaag
    15601 gacagcctct cggtcatcaa cgaccttgtc cgggcgccgg tcgtgaggga cggcgttgcc
    15661 tataccgtcg agccggcctc cacgcggagt tcgtcgctcc tggtcaccgg cgacctggat
    15721 aagatgctgc atgcccgcct gaccgagagc gacatccgtg cgattccgtt ctcggctctc
    15781 ccgtactcgc tcgggctcgg tgcactcgga gagaacgtgg cggatgcgct cccgctcctc
    15841 ggggagatga tcacgctgca cggctcggtg gtatggctgc cgaccgacgg gaacgccacg
    15901 ccggacttct tcacgccggt gaaggatgcc ggaaagccct gcatctacac agggtacaac
    15961 gtgacgcttt atgggccgtt tcacgagatc atcacgatcg acgcggggga tgagggcggc
    16021 atcgcgctct ccgacatata ccgcacgatc tttgactttg cccgggagcg aaagcgcgat
    16081 ttttccggcg tcgtcgctct tgccatgtgg gccgtcgtcg acggggtctg cagcctgggg
    16141 gtgaagcgtt cgcccatcgc cgcgtcgtcc ccggcaaacg gcctccccat caacgcggag
    16201 gagaacgcaa aagagtggct cgaggagaac gacgaaccga agtaccgcgg ggagacgatg
    16261 gtcagcttcg ggttcggtat agactactcc gcctgcagcg gcgagcacct atcggccctc
    16321 accgaccgga accgtccgaa gacgcagcag ctgcaactcc acaaccacgg cgtcatcttc
    16381 cggggggtgc cgtgggatgc gtccctcgac ctcacgcggc agatcgagcg gatcgtaacc
    16441 ggggccgagt tcgtcgatat gcgccacctc aaggacaaca cccgtatccg gcgggcgaag
    16501 gtgggcgtgg cctacatatc ggagatcgcc gtcgaagagt gagccggata cctccggaga
    16561 taaaagaggc gggggatatc cccgctcctc ctgcccgtgg gcgggaggtt tatagcccga
    16621 ggtcggacaa cccggggtga tcgtcggggc gccgcccgag cggccagtga tacttccgct
    16681 ccccctcttt gatcgggaga tcgttgatgc ttgcgtagcg gcggcgcatc aggccggact
    16741 catcgaactc ccagttctca ttcccgtagg accggaacca gttgttcgaa tcgtcgtgcc
    16801 attcgtaggc gaaccgtacg gcgatacgcg cctcgtggaa cgcccatacc tccttgatca
    16861 ggcggtagtc aagctctttt gcccattttc gctgcaggaa tgcgacaatc tcctcgcgac
    16921 cgttgatgaa ctccgatctg ttgcgccaga tactgtcgat gctgtacgca agcgagacct
    16981 tctcgggatc acgggagttc caggcatcct cagccatacg gaccttctgc accgccgtct
    17041 catacgtgaa cggcggcagt ggagggcgac tcttctcggc catgcgcccc ttctacctca
    17101 tgcaacatga taaaggtgcg gtcgataccc acgatgacgg gatggcatga caccgatcga
    17161 agtggtgcag gagatcatgg ttcgctacat ctcctattgc gcaaaatttt acgattttca
    17221 caaattccct gaccattatc cagcagtacc ctttaatcga ccaggataac cccctggtta
    17281 cttcgccaaa gtatatatgg atatagttcc gtacctggtg aacaacggac tcatttcagc
    17341 caccggaata ctattgggtc ttgcagtcag gaatcgattc acaaccgggt gccggataat
    17401 gatccagaga aatcagcttg atctgagaaa attcgtcgtt cctgagttta tctgcggata
    17461 tggagcactg caacttgcag gccggtatgc gcgtaacttc ggtatgaaac aggtgctcct
    17521 cgtcaccgat ccaggcgtcc gggccgcagg ctgggcggat gccgtcggag agagcctgac
    17581 ggaggcgggg gtaccgttca ccatctttga tgccatcagt ccaaatcccc gctcagggca
    17641 ggtgatggcg ggcgcggagg tgtatcattc cgaagaatgc aacggtatcg tcgccgtcgg
    17701 cgggggtagc ccgatggact gcgccaaggg tatcgggatc gtgagttcca acatgcagga
    17761 tatcctcacg tttgagggcg tcgaccgggt gcc
//
LOCUS       DAOSII010000030        17580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_3434_length_17580_cov_52.915835, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000030 DAOSII010000000
VERSION     DAOSII010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 17580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17580
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3434_length_17580_cov_52.915835"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..414)
                     /locus_tag="PLV90_03775"
     CDS             complement(79..414)
                     /locus_tag="PLV90_03775"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOF96262.1"
                     /translation="MRRSRGDIWFVDLADARGHEQARLRPAVVIAVAYGGMTIVVPLT
                     TTPAALSFPHTYGIEKSSQNGLTSDSAALIFQIVALSEDRFVKKIGRCSVEDMESISV
                     LLKDLLALE"
     gene            complement(416..952)
                     /locus_tag="PLV90_03780"
     CDS             complement(416..952)
                     /locus_tag="PLV90_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96263.1"
                     /translation="MPASDRSKVIACFREAGFRMDKNRFEHRLIAQKLIYLLKLKGVA
                     FCYSFHLYVRGPYSPDLAREYYQHADEFSRCETESTLSSAEADAVAGLTSLFDKSPSL
                     LEIGATYGYLAYELHHPPEQAYRTVRRMKSFYPGEQIVKGVNRAKQYLFVPADEEKAA
                     LEAELQEWQRAGIRSMRH"
     gene            complement(936..1277)
                     /locus_tag="PLV90_03785"
     CDS             complement(936..1277)
                     /locus_tag="PLV90_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOF96264.1"
                     /translation="MPDDLITIVEESEDVDSTLISRVRLEILWALSELGEDGATARQL
                     KAGLNLSDGVLYANLKKLVAMGYLRSEKVTLEGKELELYAITPEGLLEWRRIRSWLCK
                     LLDCDDDACER"
     gene            1987..2244
                     /locus_tag="PLV90_03790"
     CDS             1987..2244
                     /locus_tag="PLV90_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96265.1"
                     /translation="MLGDTQTMQQQAVMGEIEEMIQSMPDYAEVREALRSLDFIPEQD
                     DADVAIWVQPDLRIFVLLRMNLGGGYAGYKIAAYDEADTAR"
     gene            2290..2556
                     /locus_tag="PLV90_03795"
     CDS             2290..2556
                     /locus_tag="PLV90_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96266.1"
                     /translation="MHRQVTIDREEQERVRARVREVIATSPAYGDIRAGLEHLGFQAK
                     EDNPALALWENGENELFVMVHMDPDTGRLRDCEVKTFEEMEGFE"
     gene            complement(2560..3054)
                     /locus_tag="PLV90_03800"
     CDS             complement(2560..3054)
                     /locus_tag="PLV90_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96267.1"
                     /translation="MNVAMRRHFHIGGAPFLSGMGRKGSLAGILVSRLTGIILFLILI
                     GVLNVFADVYVGNPVFLRVVAFLNANVGFLILIAVIFLLGDLFCTLVFPLNLPGPIFG
                     ALGAVFVVAFIFRVFMLAGDMTGIEVFRIFSGTLAHLIYVLVFAAVLIGDYISLFSEP
                     SGRA"
     gene            3089..4831
                     /locus_tag="PLV90_03805"
     CDS             3089..4831
                     /locus_tag="PLV90_03805"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HOF96268.1"
                     /translation="MKFATFAACSEELGATSSRNDMTTLLARLLATAAPDDIAVVCYF
                     TLGDIAPGYSGVTTGIGDRTVAAAIALAAGTTPLSVEAAARETGDYGDAAARLAGAGP
                     AAEPLTVAAVHRGLMDIARAAGPGSAERKVETLAALLSAATPPERRSIVRLATGKMRL
                     GAGDMTLLDALAEAYLGSKDARAPLEHAYNISSDIGLVARTLKSGGLPAVEAISVTLH
                     RPVRPMLTQRVARLSEIPERIGSPAIAVEEKYDGERVQAHKDGDDVTLFSRRLTDVTH
                     QYPDIVRSVQEHVTAGTVILDGEAVAFDHETKTYRPFQTLMRRRRKHRVGEVAREIPA
                     TYRAFDLLYMDGASYLRRSYPDRRAALEAVLSPDENISPADRIVTGDLNAITEFYIAC
                     IERGLEGIVCKSCADDSFYRAGAREWQWIKWKSDYAPDLADTLDLAVVGANAGRGKRA
                     GTFGSILCAAYNPEEDIFQTVCAVGTGFTDADLADLSRRFAGATVDRQPARVMATPQA
                     APDFWFVPADVAEVRGLEITESPVHTCNWDPERRRGLALRFPRFVRWRDDRSPEAATT
                     TAEVAAMFRRRRGV"
     gene            4835..6568
                     /gene="polX"
                     /locus_tag="PLV90_03810"
     CDS             4835..6568
                     /gene="polX"
                     /locus_tag="PLV90_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HOF96269.1"
                     /translation="MEMLPGMTNLEVAAILHEVADLLDIKGVRFKPHAYRRAAMAIET
                     LPEDVADVAREGRLGDIPGVGRGIAEKIEEIVKTGRLAYLESLRRELPAGVQELIRIE
                     GIGPKKATVLTRDLGIRSIDDLEAAAKAGRIRDLPGFGEKTEQNILAGIRMSRTAKER
                     YLLGYILPIAREIEERLRGLAAVRQVSLAGSTRRRKETIGDLDILAASARPEEVMEAF
                     VALPGVVRVLARGTTKTSVVLATGIQVDLRVVEERQFGAALQYFTGSKEHNIALRKLA
                     IARNWRLNEYGLVDLADNRTIAGEDEAGVYRALGLAWIEPELREDRGELEAARTGTLP
                     DIVGYDAIRGDLHVHTRWSEGVHSIGDMAEAATALGYEYIAICDHAETLHIARGLSPE
                     RLADQMREIEQINREGGSVTVLAGTECNIGMDGSVDLPDSVLADLDVVVASVHSGFKQ
                     PERAMTERVVTAMHNDHVDIIGHPTGRILLTREPYRIDIAAVAKNAAALGVLLEIDAF
                     PGRLDLSDVNARLARDAGARFSLGSDAHNRENLRYMEFGVATARRGWLTADDIANTRP
                     LAGLRELLTRR"
     gene            complement(6555..6992)
                     /locus_tag="PLV90_03815"
     CDS             complement(6555..6992)
                     /locus_tag="PLV90_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOF96270.1"
                     /translation="MVQAKEGDTVKVHYTGKLEDGTVFDTSGDQDPLEFTIGGGQVIP
                     GFEQAVVGMEPGETKTATIPPEEAYGPHREDMTLTVDRSQFPEEINPEPGQQLQVQQP
                     DGRAAIVVVSDVSESTVTLDANHPLAGHPLTFDIQLVDLVSAG"
     gene            complement(7106..7660)
                     /locus_tag="PLV90_03820"
     CDS             complement(7106..7660)
                     /locus_tag="PLV90_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOF96271.1"
                     /translation="MPDGADAVYVVGEYHFDDIGPEDIVLDIGANVGAFCIRAARRSR
                     RVLAVEPVLAGALQENVRENGVSVTVVSGALGGGGDERITWGGRTLTVATRTLGEFAG
                     MIGGCDFLKCDCEGAEWSIRPADLDGVRRIEMELHVPPIGGPVNRRLLDYIGEHYDFT
                     IDRTPGYAALGVMGVLHAARRSGD"
     gene            8002..9261
                     /locus_tag="PLV90_03825"
     CDS             8002..9261
                     /locus_tag="PLV90_03825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOF96272.1"
                     /translation="MAYMDGITCICGNGRTLEDHRPIVGNDVISGIYRKAAGLRGKRV
                     LHVNSTYQSGGVAEMILSLVPLMNDVGIRTQWNILNGEGDFFVITKNFHNALQGQAIA
                     FSDEEKTLYLRTNETFSQRMAIDHDLVVIHDPQPLPLIRFYEKTQPWVWRCHVDLSNP
                     DPALWEFLKGFALDYDRMVISHEQYRRSGMPMEQWICRPAIDPLSEKNRDLSDAEIAG
                     LLAKYGVPTDKPLVTQISRFDKWKDPEGVVDVFCEVRRHVDCRLVLCGSMAPDDPEGW
                     AIYRRVEEAARPLIDAGDVVLITAEDHLLVNALQRASAVILQKSLREGFGLTVTEGLW
                     KGRPVIASRVGGIPLQIEDGETGFLLDPTDTEGFAWRIRQVLENPELGERLGRAGKEH
                     VRQHFLITRLLSDYLDMLNAELIGRNA"
     gene            complement(9766..11325)
                     /locus_tag="PLV90_03830"
     CDS             complement(9766..11325)
                     /locus_tag="PLV90_03830"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOF96273.1"
                     /translation="MENNITFKEFNISQKMLRAIEDLGFEEPTPIQVSTIPAILEGRD
                     VTGQAQTGTGKTAAFGIPAIERIDPESRETQVLVLSPTRELAIQIAEEFARLAKHHRG
                     INILPIYGGQPIERQFKGLQRGAQIVVGTPGRVLDHLDRGTLSFAAVNLVVLDEADQM
                     LDMGFREDIEKILDETPEGRQTVLFSATLPKPILEISKRFQKDPEFISVARREVTVPQ
                     IEQLYLEVRSRDKLEILSRLLDMYDPDLTLIFTNTKRGVDDLTTHLQARGYFAEGLHG
                     DMKQTLRDRVMAKFRAGSIDILVATDVAARGIDVEDVDLVINYDVPQDIEYYIHRIGR
                     TARAGRAGRAVTFVGPKEYFKLRTIQNYTRIQIARIPLPTPGDVEESRTRKLIDRVQQ
                     VIDEGSLERYVNLVERITSEDYTSLEVAAALLKLELGAGGQGPAQETGLALLRIPLGR
                     EHQVRPKDIVGALAGETGIPGGAIGAINIYETYSTVEVPQGAVDQVIEGMKGKTIAGI
                     RLPGPIETAGR"
     gene            11421..11858
                     /locus_tag="PLV90_03835"
     CDS             11421..11858
                     /locus_tag="PLV90_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96274.1"
                     /translation="MAARHPPPVSAADMREVYRRYAADRPGLRGILLPDLLPHDACGA
                     SPALGRIRVLFFAESPPWAAGPSDCLSPDYPYFYDDRYDAGQKRRSPAVLRRTSLLCS
                     VSTAGRAARTWRPSPGRDATSSTWSGAPSERTEKRPYRPTSSG"
     gene            complement(12119..12814)
                     /locus_tag="PLV90_03840"
     CDS             complement(12119..12814)
                     /locus_tag="PLV90_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOF96275.1"
                     /translation="MAWRRMAREPWGEFDDMIANMQRQFEETMGRISGAAQQVALPGG
                     IRTTVDVREDETDVLVVADLPGVERQDISVRLTDPRTLRITARREMIHEGEETGYYVR
                     ERRVGAIARTVSLPADVTGEGTATFRNGVLEVRLKKIAEARGREIEVGGTSGKAALSA
                     AEEQRQQKEKLYREDREKVEPSGYLDPREIAKEARDIQLEEPGSPEERSTAARLRKRK
                     EELYAEGKKKLAG"
     gene            13010..16777
                     /locus_tag="PLV90_03845"
     CDS             13010..16777
                     /locus_tag="PLV90_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HOF96276.1"
                     /translation="MFRFNLSPSLIGRFFYHDCERYLRYHATPEQERENAGIPAIAVD
                     TSPTTRALLDAGIRWEEEVVRTRLSGRVRVPGGTGPISERSFSIEESFGLIPSLSPGD
                     AIYQTTIPVSVHFLKGYGLDPDMHRFSPCRPDLIRVDEEGRLSVIDIKASEDLSVSHR
                     VQATLYALILDHALDLLGISVPVDLSRAGIWLYGKDEPEYFDLSLNIRVIEDFFRHRL
                     PGILAGPPEDVPWHITPRCEACEFYPHCRAGAEASSSVSLIPGLSPTARRYLREAPWD
                     GGLSINTLADLAAFLRDPASDRHLDDCGSLAGRGDRLRATVRALATGEVVRLAGTSLA
                     LPVYEDVAIILSLQKDPVSGGVYALGFRRSRGEAVYGTPSHEAIYVAADEGDCARVRR
                     EFIRALAAELAAVHDYNQGREWADQKSVQTYVYDATEGELFARSIREGLFDPATAEDA
                     LRLQFTYQETDIARGSSHPAGSVAFPLVVITGEIRRMLALPAPFALRLPEVLAIIPSS
                     RFAYTLSASSLFWNEQSNAMKGDAVIMAWSGRAEAAGWIRQEVSRRLLAAGSILDGLR
                     ERVKAGLVRWPENFRFPRPWDAATPELSRLLFVTEYESTLAARRMQELRSRPWPVRVR
                     EGISIPLEKSEGNFWKVRTPLDLALFEQSQAFGHLLVPDGDAGEEAQLGFDDLRYRDS
                     PNPGKSGVSFARVHDSIVDREAGIVRGLVLTVNVGRDRTPFVEGDRAVLHPRFTDFTA
                     PRYVDRLLALDRQPENTFVRLLRDPRGFAAPVAETEAVIADAEQTVLDAGFTKSQARA
                     FHRVMTGRLTLVWGPPGTGKTHFLATAILALVRARRAHGERIRVGVTAFTHAAIENLL
                     VRVQDSVDEFGLAAGFPIYKLKDARTSRGERSLKVLPHDRADTVVGYPALLLGGTVHS
                     FERLEKSLPSLDLLIVDEASQMRPADLAMVLPLLADGGRLVLAGDDLQLPPVIQGEYP
                     EPEDGLPGLWDSIFAYLRHRDDPGRYTCQLQENWRMNRTLSRFPAETLYGAGYAPAND
                     AVGEQRIALAPGLPGEEWIAWALDPAYPLVLCILENVRTTVENQVEAGLVARLACTLR
                     ERLLDPASGNPYPATADGDALFWRRGLFVVSPHHAQIGAIRDCLSRERAWESRPFVDT
                     VDKMQGQEAEAVIVSYGVSDVETALSEAGFIYSRNRLNVSLTRGRSKCVVFLPRPLLE
                     PPLDLVGDPTAAAGLRHMLDLQEFCRTHGEEKTFSGDARGVRLTVMRARVE"
     gene            16820..>17580
                     /locus_tag="PLV90_03850"
     CDS             16820..>17580
                     /locus_tag="PLV90_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOF96277.1"
                     /translation="MIGSLDPDQNEREQRILKETREHGATPEAVRLFRALVLAHYRAH
                     GRDLPWRQTTDPYRILVSEIMLQQTQVERVAVKYPEFIDRFPDFASLARAPQSDILLA
                     WQGMGYNRRALALKRTAERVVDDYGGRLPADVETLATFPGIGRATAAAICAYAFNMPV
                     AYIETNIRRVFIHFFFQDREDVRDDEILVLVEQTLDRENPREWYGALMDYGSILKKRT
                     ANPNRRSASYSRQSRFEGSDRQIRGRLLALVLAEGA"
BASE COUNT         3061 a         5855 c         5638 g         3026 t
ORIGIN      
        1 tccaccgaga tctattatca ggtatatgcc ggaggcactg cgctctggct gcttcgatga
       61 atccccggga acaaaccgct actccagcgc caacagatct ttcaaaagaa cgcttatcga
      121 ttccatatcc tcgaccgaac accgcccgat ctttttgacg aagcgatcct ccgagagggc
      181 aacaatctgg aatatcagcg cggcactatc tgaagtaagc ccgttctgtg agctcttctc
      241 gatgccgtac gtgtgcggga acgacagagc cgccggggtc gtcgtcaacg ggaccacgat
      301 ggtcataccc ccgtaggcga cggcgatcac cacggcagga cgcaaacggg cttgctcatg
      361 cccccgtgca tcggcgaggt cgacaaacca gatatcgcct ctgctccggc gcatcctaat
      421 gcctcatcga ccggataccg gcccgctgcc actcctgcag ttcggcttcg agcgccgcct
      481 tctcttcatc ggcggggaca aagaggtact gtttcgcccg gttgacgccc ttgacgatct
      541 gctcccccgg gtagaacgac ttcatcctcc gaaccgtccg gtaggcctgc tcgggcgggt
      601 ggtgcaactc gtaggccagg tacccataag tcgccccgat ctcaagcagg gacgggcttt
      661 tatcgaagag gctggtgagc ccggcaacgg cgtccgcctc agcggaagag agcgtgctct
      721 ccgtctcgca ccgggaaaat tcatcggcat gctggtagta ctcccgtgca aggtccggcg
      781 agtagggccc ccggacgtag aggtggaacg agtagcaaaa cgccacccct ttcaacttca
      841 gcaggtagat gagtttctgg gcgatgagcc ggtgctcgaa ccggtttttg tccatccgga
      901 atcccgcttc ccggaaacag gcaatgactt tgcttctatc gctcgcaggc atcgtcatca
      961 cagtccagga gtttgcagag ccagcttcgg atacgccgcc actcaagaag tccttccggg
     1021 gtgatggcgt agagttcgag ttcctttcct tcgagcgtca ccttctcgga gcggaggtac
     1081 cccatagcga cgagtttttt gaggtttgca taaagcacgc cgtcgctcag gttcagcccg
     1141 gccttgagct gccgtgcggt ggccccgtcc tccccgagct cggagagagc ccagagtatc
     1201 tccaggcgca cccgggagat gagggtgctg tccacgtctt cggactcttc cacgattgtt
     1261 ataagatcat caggcatagg cgacttttag ttttgagtag atcttggagt tagaggagat
     1321 aaaggattcg ggcggtgttt cttatgtgtc agcgggttac tatacccaca caaggacacg
     1381 acgcagtcaa ctaccccgcg ccttttagga ggggcttgta acaaggttaa atgtccttaa
     1441 agcgtcacaa ttctgaaggc atgggatcgt gctatcggac gatctcatgg gctcgaggga
     1501 aaccaaactg gcaaacttag gttgcgagca acttcatcga gcctaaaaaa ggcatgacgc
     1561 cagcaatgag ggaagatact acggatcgaa tatggacctg gaaggagctc ttgacgtatc
     1621 attttcagtt ataacttaga gcactacccc gtttctgcat cttatcacct ccatcccgaa
     1681 gcttccggca taaccgggaa aatggacgtg tacgcgtatt gcaggtgcaa aacgatagcc
     1741 ctttctgaac gcagggggaa ggggcgggag tttgaggggc cttgagcaac gacatcaaga
     1801 gaagggttgc ggcgcctgcg aggagcgagc atcaggtatc tccccgatcg ttcccgcaca
     1861 gcccggccgc ccctcgtatt cccccggaga aagaagagtc gccggcagcc ccgtgcgagc
     1921 cgataccacg ccacccgcct tcacccccgg atcaccttta tgccggatgc cggcaacggg
     1981 gggtccatgc tcggcgatac ccaaacaatg cagcagcagg cggtgatggg cgagatcgag
     2041 gagatgatcc agtcgatgcc cgattacgcc gaagtcaggg aggctctccg ctcgctggac
     2101 ttcataccgg agcaggatga cgccgacgtc gcgatctggg tccagcccga cctcaggatc
     2161 ttcgtcctgc tccggatgaa cctcgggggc ggatacgcgg gttacaagat tgccgcctac
     2221 gatgaggccg acaccgcccg gtgatcgccg caggtcaggt ttatcccggc gtacgcagta
     2281 gggggagcca tgcacagaca ggtaaccatt gaccgggagg agcaggagag ggtgagggcc
     2341 cgggtccggg aggtcatcgc cacgagccct gcctacggcg atatccgggc gggcctcgaa
     2401 cacctcgggt tccaggcaaa agaggacaac cccgctctcg ccctctggga gaacggcgag
     2461 aacgaactct tcgtcatggt ccacatggac cccgacaccg gccggctgcg ggactgcgag
     2521 gtgaagacgt ttgaggagat ggaggggttc gagtaacgat cacgccctgc cggacggctc
     2581 cgagaagagc gatatgtagt cgccgatgag gacggcggcg aagacgagga cgtagatcag
     2641 gtgcgcaagc gtccccgaaa agatcctaaa aacctcgatg cccgtcatat cgcccgcgag
     2701 catgaagacc cggaagatga aggcgaccac gaagacggcg ccgagggcgc cgaagatcgg
     2761 ccccggcagg ttcaggggga atacgagggt gcagaagagg tcgccgagca gaaagatcac
     2821 cgcgatcagg atgaggaacc cgacgttggc gttgaggaac gcgaccacgc ggagaaagac
     2881 cggattcccg acataaacgt cggcgaaaac gttcaggacg ccgatcagga tcaaaaagag
     2941 gatgatcccg gtgagcctcg atacgaggat gccggcaagt gatcccttcc tgcccatccc
     3001 ggagaggaag ggcgccccac ctatatggaa gtgtcgtcgc atcgcaacgt tcatctctct
     3061 cccggccgtg atcactcatg agagccccgt gaagtttgca acctttgccg cctgttcgga
     3121 ggaacttggg gccacgtcgt ccagaaacga catgaccacc ctccttgcgc gtctgctcgc
     3181 cacagcggcg ccggacgata tcgccgtcgt ctgctacttc acgctcggcg atatcgcccc
     3241 cggctacagc ggagtgacca cggggatagg ggaccggacg gtggcggcgg ccatcgccct
     3301 tgcggccggg accacacccc tctccgtcga ggcggcggcc cgggagaccg gagattacgg
     3361 cgacgccgcc gcccgactgg ccggggcagg ccccgccgcc gaacccctca cggtcgcggc
     3421 cgtccaccgg ggcctcatgg atatcgcacg ggcagccggc cccggatcgg ccgagagaaa
     3481 ggtagaaacc cttgccgcgc tcctttctgc cgcgacgccg cccgagcggc gctccatcgt
     3541 ccggctcgcc accgggaaga tgcggctcgg cgccggggat atgacgctcc tcgatgcgct
     3601 cgcggaggca tacctgggct cgaaggatgc ccgcgccccc ctcgaacacg cctacaacat
     3661 atcctccgac atcgggctcg tggcccggac gctcaaaagt ggaggcctcc cggccgtgga
     3721 ggcgatatcc gtcaccctgc accgccccgt aaggccgatg ctcacccaga gggtcgcccg
     3781 gctatcggag atcccggaac ggatcggctc gccggccatc gccgtcgagg agaagtacga
     3841 cggcgaacgg gtccaggcgc acaaagacgg cgacgacgtc accctgtttt cacggcggct
     3901 gaccgacgtc acccaccagt accccgacat cgtccgaagc gtgcaagagc acgtcacggc
     3961 cggcacggtg atactcgacg gcgaagcggt cgcgttcgac cacgagacca aaacgtaccg
     4021 ccctttccag acgctcatgc gccggcgccg gaagcaccgg gtcggagagg tcgcaagaga
     4081 gatccccgcg acctaccggg cctttgacct cctctacatg gacggcgcat catacctccg
     4141 gcgaagctac ccggaccggc gggcggcgct cgaggcggtc ctctcccctg acgagaatat
     4201 ctcccccgcg gaccgcatcg tgaccggcga tctcaacgcg atcacggagt tctatatcgc
     4261 ctgcatcgag cgggggcttg aagggatcgt ctgcaaatcg tgcgcagacg actcgttcta
     4321 ccgcgcggga gcgcgggaat ggcagtggat caagtggaag agcgattacg ccccggatct
     4381 cgccgatacg ctcgacctcg ccgtcgtcgg cgccaacgcc gggcgcggga agcgggccgg
     4441 gacgttcggc tcgatcctct gcgccgccta caaccccgaa gaggatatat tccagacggt
     4501 ctgcgccgta gggacggggt ttacggacgc agacctcgcc gacctctccc ggcggttcgc
     4561 cggcgcaacg gtagaccggc agccggcccg ggtgatggcg acaccgcagg cggcgcccga
     4621 cttctggttc gtgccggcag atgtggcgga ggtgcggggg cttgagatca ccgagagccc
     4681 ggtccacacc tgcaactggg acccggagcg gcggcggggg cttgccctcc ggttcccgcg
     4741 gttcgtgcgg tggcgggacg acaggagccc tgaagcggcg acgacgaccg cagaggtcgc
     4801 ggccatgttc cgccgccggc ggggggtata aggcatggag atgctccccg gcatgaccaa
     4861 cctcgaggtc gcggcgatcc tccacgaggt cgccgacctc ctcgatatca agggcgtccg
     4921 gttcaaaccg catgcctatc ggcgggcggc gatggcgatc gagaccctgc cggaagatgt
     4981 cgccgacgtc gcgcgggagg ggcggctcgg cgatataccc ggtgtcggcc ggggcatcgc
     5041 cgagaagatc gaggagatcg taaagaccgg gagactggca taccttgagt ccctgcgccg
     5101 cgagctcccg gcaggcgtgc aggaactcat ccggatcgag gggatcggcc cgaagaaggc
     5161 gaccgtgctc acccgggacc tcggtatccg gtccatcgac gacctggagg cggcggcgaa
     5221 agcgggccgg atccgcgacc tcccggggtt tggggagaag accgagcaga acatactcgc
     5281 cggcatccgg atgagccgga cggcaaaaga acgctacctg ctcgggtaca tcctccccat
     5341 cgcccgcgag atcgaggagc gcctccgggg gctcgccgcg gtccggcagg tgagcctcgc
     5401 tggctcgaca cggagaagga aggagacgat cggggacctc gatatcctcg cggcatcggc
     5461 acgcccggag gaggtgatgg aggccttcgt cgcgctcccc ggcgtcgtgc gggtccttgc
     5521 ccgggggacg acgaagacgt cggtggtgct tgcgaccggt atccaggtcg acctccgggt
     5581 ggtggaggag agacagttcg gggccgccct ccagtacttc acgggttcaa aggagcacaa
     5641 catcgccctc agaaagctcg cgatcgcgcg gaactggcgg ctcaacgagt acggcctcgt
     5701 ggacctcgcg gacaaccgca cgatcgcggg ggaggacgag gccggcgtct accgggcgct
     5761 cggtcttgcc tggatcgagc cggaactcag ggaggaccgc ggcgagctcg aggccgcccg
     5821 gaccgggacc ctgcccgaca tcgtcggcta cgacgctatc cgcggcgacc tccacgtcca
     5881 cacccggtgg agcgagggcg tccactccat cggcgatatg gcagaggccg cgacggcgct
     5941 cggctacgag tacatcgcca tatgcgacca cgcggagacc ctccatatcg cccgcggcct
     6001 ctcccccgag cgcctggccg accagatgcg cgagatcgag cagatcaacc gcgaaggcgg
     6061 cagcgtcacc gtcctcgccg gcaccgagtg caacatcggc atggacggca gcgtcgacct
     6121 tcccgacagc gtccttgccg acctcgacgt cgtcgtggca agcgtccatt cggggttcaa
     6181 gcagcccgag cgggctatga cggaacgggt cgtcacggcg atgcacaacg accacgtcga
     6241 catcataggc cacccgacgg ggaggatcct ccttacgcgg gagccctacc ggatcgatat
     6301 cgccgcggtc gcgaagaatg cggcggcgct cggcgtcctc ttagagatcg acgccttccc
     6361 cggcaggctg gacctctccg acgtcaacgc acgtctcgcg cgggatgccg gcgcccggtt
     6421 ctcgctcggc tcggatgccc acaaccgcga gaacctccgc tacatggagt tcggcgtcgc
     6481 gaccgcccgg cggggatggc tcacggcgga cgatatagcc aacacccgcc cgcttgcggg
     6541 tctgcgggag ctcctcaccc ggcgctgacg agatccacga gctggatgtc gaacgtcagg
     6601 ggatgcccgg ccagggggtg gtttgcgtcc agcgtcacgg tcgattccga gacgtcgctg
     6661 acgacgacga tggccgccct gccgtccggc tgctgaacct ggagctgctg gcccggttcg
     6721 gggttgatct cctcggggaa ctggctccgg tcgaccgtga gcgtcatgtc ctcgcggtgg
     6781 gggccgtagg cctcctcggg cggtatcgtg gcggtcttcg tctcgcccgg ctccatgccg
     6841 acgacggcct gttcgaaccc gggtatgacc tgtcctcccc cgatggtgaa ctcgagggga
     6901 tcctggtcgc ctgacgtatc aaagaccgtg ccgtcctcca gttttcccgt gtagtgcacc
     6961 ttgacggtgt cgccttcttt tgcctgcacc atgctgcttc accggcgggg tggtgaacag
     7021 gaatcctatt tatgcatgcc ggccggaccc gaacaaaccg ggatcgtgcg cctttccggt
     7081 gacaggaacg gcggccggcg atcggttagt ctccgctccg ccgggcggca tgcaggacgc
     7141 ccatgacgcc gagtgcggcg tacccgggcg tccgatcgat cgtgaagtcg tagtgctccc
     7201 cgatgtagtc gaggagcctg cggttcaccg gcccgcctat cggggggacg tggagctcca
     7261 tctcgatgcg gcgtactccg tcgaggtccg ccggccggat cgaccactcc gccccctcgc
     7321 agtcgcactt caggaagtcg cacccgccga tcatgccggc aaactcaccg agggtccggg
     7381 tcgccacggt gagggttctg cccccccagg tgatccgctc atcgcccccg ccgccgagtg
     7441 ccccggagac gaccgtgacc gagacgccgt tttccctgac gttctcctgc agagccccgg
     7501 ctaggacggg ttcgaccgcg agaacccgcc gcgaccggcg ggcggcgcgg atgcagaaag
     7561 cgccgacgtt cgccccgata tcgagaacga tatcctccgg cccgatatcg tcgaaatggt
     7621 actcgccgac gacgtagacg gcgtcggccc catcaggcat cctccgaaag gttacgccgt
     7681 cggaagtgac gacccgggag gggtcttttg gggcacgctc tcggtacacg gaatagtatt
     7741 ggaatttcgg tatacatggg cgtttctccc cgggtgcgcg ccaccccatc tgcggccccg
     7801 gagggcctga atcaggccat cttattaatc ttttccggca tattccaatc gtcgcaggag
     7861 tgcggaattt catttccgtc cggtgtgcga gacggtgccg gtgtgccgcg ccggtaaaaa
     7921 ctccagttat atataaatat atttataaat ttagaagtgt aaataatcca tgtagcacaa
     7981 ccacttgagg ggagatctgc tatggcctac atggatggaa taacctgtat ctgcggcaac
     8041 ggccggacgc tcgaggacca ccggcccatc gtcggcaacg acgtcatctc cgggatctac
     8101 cgaaaagcgg cgggcctccg gggaaaacgg gtcctccacg tgaactcgac ctaccagagc
     8161 ggcggcgtgg cggagatgat cctctcgctc gtcccgctca tgaacgatgt cggcatcagg
     8221 acgcagtgga acatactgaa cggcgaaggc gacttcttcg tcataacgaa gaacttccac
     8281 aacgccctcc aggggcaggc gatcgcgttc tcggacgagg agaaaaccct ctacctccgg
     8341 acaaacgaga cgttctcaca gagaatggcg atcgaccacg acctcgtggt catccacgac
     8401 ccgcagcccc tgcctctgat ccggttctac gagaagaccc agccctgggt ctggcggtgc
     8461 cacgtcgacc tctcgaaccc cgaccccgcc ctctgggagt tcctcaaggg cttcgccctc
     8521 gattacgacc ggatggtcat atcgcacgag cagtaccggc gctccggcat gcctatggag
     8581 cagtggatct gccggccggc aatcgacccg ctctcggaga agaaccgcga cctctccgac
     8641 gccgagatcg ccggcctcct tgcgaagtac ggggtgccga ccgataaacc tctggtcacc
     8701 cagatatcgc ggttcgacaa gtggaaggac cccgaaggcg tcgtcgacgt cttctgcgag
     8761 gtcaggcggc acgtcgactg cagactggtc ctctgcggga gcatggcccc cgacgacccc
     8821 gagggctggg cgatctaccg gcgggtcgag gaggcggcgc ggcccctcat cgatgccggc
     8881 gacgtcgtcc tgatcaccgc cgaggaccac ctgctggtca acgccctcca gagggcatcg
     8941 gccgtcatcc tccagaagtc gctccgcgag gggttcggcc tgaccgtcac cgaagggctc
     9001 tggaaagggc ggccggtcat cgcctcccgc gtcggcggca taccgctcca gatcgaggac
     9061 ggcgagaccg ggttcctcct cgacccgacc gatacggagg ggttcgcctg gcggatccgg
     9121 caggtccttg agaacccgga actcggcgag aggctcggcc gggccggcaa agagcacgtc
     9181 cggcagcatt tcctgatcac ccgcctcctc tccgactacc tggatatgct caacgccgag
     9241 ctcatcggcc ggaacgcctg aagaataaag cgatacgcga gatccggagg gaccggtttc
     9301 cccggaatcg gtctctccgg aaaggtgcgc ggcggggtgc cccgccgcgt ccgggatgca
     9361 ggataggcgg cgcaggtgcc ggctcaacat ggtcagcggt gctggatgcc gggtattctt
     9421 ttctatcaat atcctgttgt gcacatgcgg aacacagggc aggtactatg gaagttttcg
     9481 atggcgatga gactccggaa gaattcagat atcacgtcca accggttgac gcaaccaccc
     9541 cccaatgatg acaggatata catccggaca tataattgtt tctcacgccg gcgaacggtg
     9601 ccggtagcca tggggtctcc ttcacgctcc cggttgtcgt gaggtacccc cggcggcgct
     9661 cgccgggtgc gccggggttt ctccaaaaaa ggttccggga gtgcgaaaga tactcgtctg
     9721 cggcaccgcc cgccggcctg aaaaggatga aacccggggc caccctcacc ggccggcggt
     9781 ctcgatcggg ccgggcagcc tgataccggc gatggtcttt cccttcatcc cctcgatgac
     9841 ctggtcgacg gcaccctggg gcacctcgac ggtggagtaa gtctcgtata tgttgatcgc
     9901 gccgatggcg ccgccgggta tgccggtctc gcccgcgagc gccccgacga tatccttcgg
     9961 ccggacctgg tgctctcttc caagcggtat ccggaggagt gcgagcccgg tctcctgcgc
    10021 cggcccctgg ccgcccgcac cgagctccag tttcaggagg gcggcggcga cttcaagcga
    10081 cgtgtaatcc tcagacgtta ttcgttcgac caggttcacg tagcgctcga ggctgccctc
    10141 gtcgatgacc tgctgcaccc ggtcgatcag ttttcgcgtc cggctctcct cgacgtcccc
    10201 cggcgtcggg agcgggatgc gtgcgatctg gatccgggtg tagttctgga tcgtccggag
    10261 cttgaagtac tcttttgggc ccacgaacgt gacggcccgc ccggcccgcc cggcgcgtgc
    10321 cgtccggccg atgcggtgga tgtagtactc gatgtcctgc gggacgtcgt agttgatgac
    10381 caggtcgacg tcctcgacgt cgatgccccg ggcggcgaca tccgtcgcga cgaggatgtc
    10441 gatggacccg gcgcggaact ttgccatgac ccggtcccgc agggtctgtt tcatatcccc
    10501 gtggagacct tcggcaaagt agccccgggc ctggaggtgg gtggtcaggt cgtcgacgcc
    10561 ccgcttggtg ttcgtgaata tgagcgtcag gtccgggtcg tacatgtcga gcaggcgcga
    10621 gaggatctcg agtttgtccc ggctgcgcac ctcaaggtag agctgctcga tctgcgggac
    10681 ggtcacctcg cggcgcgcga ccgatatgaa ctcggggtcc ttctggaacc tctttgatat
    10741 ctctaagatg ggtttaggga gggtggccga gaagaggacc gtctggcggc cttccggtgt
    10801 ctcgtcgagt atcttctcga tgtcctcccg gaaacccata tccagcatct ggtccgcttc
    10861 gtcaaggacg acgaggttca ccgcggcaaa cgagagggtc ccgcggtcga ggtggtcgag
    10921 cacccgcccc ggtgtcccga cgacgatctg ggcgccgcgc tgcagcccct tgaactgccg
    10981 ctcgataggc tggccgccgt atatcgggag tatgttgatg ccccggtggt gctttgccag
    11041 gcgggcgaac tcctcggcga tctggatggc gagctcccgc gtgggggaga ggacgagaac
    11101 ctgggtctcc cggctttcgg ggtcgatccg ctcgatcgcc gggatgccga acgccgcggt
    11161 cttcccggtc ccggtctggg cctggccggt cacgtcgcgc ccttcgagta tcgcgggtat
    11221 cgtgctcacc tggatgggcg tgggctcctc aaaacccaga tcctctatcg cccggagcat
    11281 cttttgcgat atattgaact ccttgaatgt gatattgttc tccattcatc acttcttggt
    11341 gttgctacct ctttatccct gcgggacacc ggtggcccga gagcggtgat ctctatccgg
    11401 tcggcaaaga gactaccagt atggctgcac gacacccccc gcccgtgagc gccgcggata
    11461 tgcgcgaggt ctaccgccgg tatgcggccg accgcccggg tctccgcggc atcctcctcc
    11521 ccgacctcct gccgcacgac gcctgcggcg caagcccggc tcttggccgc atacgggtgc
    11581 tctttttcgc cgagtccccg ccctgggccg ccgggccttc cgactgcctg agccccgact
    11641 acccctactt ctatgacgac cgctacgacg ccgggcagaa acgccgctct ccggcagtct
    11701 tgcggagaac ctctttactc tgctcggtct cgacggcggg acgcgccgcg agaacctgga
    11761 ggccttcgcc gggaagggat gctacctcgt cgacatggtc cggcgcgcct tccgaaagaa
    11821 ccgaaaagcg gccataccgg ccgacctcat ccgggtgagc gcgagggata tcctcgcccg
    11881 cgagttcaag gcgctctccc cggattacgt cgtcgccaaa cacggcgctt gccggtctcc
    11941 gggagatcga gccctgcgcg agcgctcttc gtgatgccgg gaagatcacc gcgatcggtg
    12001 ccgaccgccg ggagcaccgt ctcctctgcg taccctaccc cggcgggcgg aaccggcgct
    12061 acctgggcgc gatcgagtcg ggatttcggg ccatcgggga tcttctgccc tgatccgcct
    12121 atccggcaag cttcttcttt ccctctgcgt agagctcttc tttgcgcttg cggagccgtg
    12181 cagccgtcga tcgttcttca gggctccccg gctcttccag ctggatatcc ctggcctctt
    12241 tcgcgatctc ccggggatcc aggtacccgg acggctccac cttctcccgg tcctcccggt
    12301 agagtttttc tttctgctgc ctctgctcct ctgcagcgga cagggcagcc ttcccggagg
    12361 ttcctcccac ttcgatctct ctcccgcggg cctcggcgat cttcttcaac cgcacctcga
    12421 ggacaccgtt ccggaaggtg gctgtcccct ctccggtgac gtcggcgggc agggagaccg
    12481 tccgggcgat cgcaccgacc cttcgctccc gcacgtagta cccggtctcc tccccctcgt
    12541 gtatcatctc ccggcgtgcg gttatccgca acgtcctcgg gtcggtgagc cgaaccgata
    12601 tgtcctggcg ctcgacgccg gggaggtcgg cgacgaccag aacgtccgtc tcgtcctccc
    12661 ggacgtcgac cgtcgtccgg atgccgccgg gaagcgccac ctgctgcgcg gctcccgata
    12721 tgcgtcccat cgtctcctca aactgcctct gcatgttcgc gatcatatcg tcgaactcgc
    12781 cccagggttc ccgggccatt cttctccatg ccatggatta ctctctcctg cctctcggcg
    12841 ccctcgggtt gtgaacacat gatatataca ttcctgtgcg gggggtgccg ccgcaccgtc
    12901 ctgccgtgcg cggcaagaga ggtcagccgg tgccccgccc tcaccggatc tcagcaatac
    12961 caactgtttt cccgcgaccg cacaaacaat gagtcactcg tgatgccgca tgttccgctt
    13021 caacctcagc ccgtcgctca tcggccggtt cttctaccac gactgcgagc gctacctccg
    13081 ttaccacgcg acgccggagc aggagcgcga gaacgccggc atcccggcga ttgcggtcga
    13141 cacgagcccg acgacccggg cgctcctcga tgccggcata cggtgggaag aagaggtggt
    13201 ccggacgagg ctttcgggac gggtgcgggt cccgggcggg acggggccga tatctgagcg
    13261 atcgttctcc atcgaggaga gtttcggcct catcccgagc ctctccccgg gcgacgcaat
    13321 ctaccagacg accataccgg tctcggtcca cttcctgaag ggctacggcc tcgacccgga
    13381 tatgcaccgg ttctccccct gccgccccga cctcatcagg gtcgacgaag aagggcgcct
    13441 ctctgtcatc gacatcaagg caagcgaaga cctcagcgtc agccaccggg tccaggcgac
    13501 cctgtatgcc ctgatactcg accacgccct cgacctgctc ggtatcagcg tcccggtcga
    13561 cctctcccgg gcggggatct ggctctacgg caaggacgaa ccggagtact tcgacctctc
    13621 cctcaacata cgggtgatcg aggacttctt ccgccaccgc ctccccggca tccttgccgg
    13681 cccgccggag gacgtgccct ggcacataac gccgcggtgc gaagcctgcg agttttaccc
    13741 tcactgccgc gccggggcag aggcctcttc atcggtatcg ctgatcccgg gcctctcgcc
    13801 cacggcacgg cggtacctcc gggaagcccc gtgggacggc gggttatcga tcaacaccct
    13861 cgccgacctc gccgcgttcc tccgcgaccc ggcaagcgac cgccacctcg acgactgcgg
    13921 gtcgcttgcg ggccggggcg accgcctgcg ggcaaccgtc cgggccctcg ccacgggaga
    13981 ggtcgtccgg ctcgccggga cgtcgcttgc cctccccgtc tacgaggatg tcgccatcat
    14041 cctctcgctc cagaaggacc ccgtctccgg cggggtctac gccctcgggt tccggcggag
    14101 caggggcgaa gcggtctacg ggacgccctc gcacgaggcg atctatgtcg cggcggacga
    14161 gggcgactgc gcccgggtgc ggcgggagtt catccgggcg ctcgccgccg aactcgcggc
    14221 cgtccacgac tacaaccagg gccgggagtg ggcggaccag aagtcggtgc agacctacgt
    14281 ctacgacgct accgaggggg aactcttcgc ccggtcgatc cgggaggggc tctttgatcc
    14341 cgcgacggcg gaggatgcgc tcaggctgca gttcacctac caggagaccg atatcgcccg
    14401 ggggtccagc cacccggcag gctcggtagc gttcccgctc gtcgtcatca cgggggagat
    14461 acggcggatg ctcgctctgc ccgccccctt tgccctccgc ctgccggagg tccttgcgat
    14521 cattccctcc tcccggtttg cgtataccct cagcgcaagc agcctcttct ggaacgagca
    14581 gagcaacgcc atgaagggcg acgcggtcat catggcgtgg agcgggcggg cggaggccgc
    14641 gggctggatc cggcaggagg tctcgcgccg gctgcttgcg gcggggagca tccttgacgg
    14701 tcttcgcgag cgggtgaagg ccggcctcgt gcggtggccg gagaacttcc ggttcccgcg
    14761 cccgtgggat gccgcgacgc cggagctatc gcgcctcctc ttcgtgacgg agtacgagtc
    14821 gacgcttgcc gcccggcgga tgcaggaact gcggagcagg ccgtggcccg tgcgggtccg
    14881 ggaggggatc agcataccgc tcgagaagag cgagggaaac ttctggaagg tgcgaacccc
    14941 cctcgatctc gctctcttcg aacagtcgca ggcgttcggg cacctcctcg tgccggacgg
    15001 cgatgcgggg gaggaggcgc agctcgggtt tgacgacctc cgctaccggg atagcccgaa
    15061 ccccgggaag agcggtgtct cctttgcccg ggtgcacgac agcatcgtcg accgggaggc
    15121 gggcatcgtc cgggggctcg tcctcacggt gaacgtcggc cgggaccgca cgccctttgt
    15181 cgagggcgac cgtgcggtgc tgcatccccg gttcaccgac ttcaccgcgc cgcgttacgt
    15241 cgaccgcctc ctcgccctgg accgccagcc ggagaacacg ttcgtccggc tcctccgcga
    15301 cccgcggggg tttgccgccc cggtcgcgga gacggaggcg gtcatagcgg acgcggagca
    15361 gaccgtcctc gacgccgggt tcacgaagag ccaggcccgg gcgtttcacc gggtgatgac
    15421 gggccggctc acgctggtat gggggccgcc ggggaccggg aagacgcatt ttctcgcgac
    15481 ggcgatactc gccctcgtca gggcccggcg ggcgcacggg gagcggatcc gggtgggggt
    15541 tacggcgttc acccacgccg ccatcgagaa cctgctcgtc cgggtgcagg actcggtcga
    15601 cgaattcggg cttgccgcgg ggttcccgat atacaagctg aaggatgccc ggacgagccg
    15661 gggcgagcgg agcctcaagg ttctgcccca cgaccgggcc gacaccgtcg tcggctaccc
    15721 ggccctcctc ctcgggggga cggtgcacag tttcgagcgg ctcgagaagt cccttccgtc
    15781 tcttgacctc ctcatcgtcg acgaagcgtc ccagatgagg ccggcagacc tcgcgatggt
    15841 cctccctctg ctcgccgacg gcggaaggct cgtcctcgcg ggcgacgacc tgcagcttcc
    15901 cccggtcatc cagggggagt acccggagcc ggaggacggt ctcccgggac tctgggactc
    15961 gatatttgcc tacctccggc accgcgacga tcctggccgc tacacctgcc agctccagga
    16021 gaactggcgg atgaaccgga ccctctcgcg gttcccggcg gagacgctct acggcgccgg
    16081 ctacgctccc gcgaacgatg cggtcgggga gcagcggatc gcccttgccc cgggcttgcc
    16141 gggcgaggaa tggatcgcat gggcgctcga tccggcatac ccgctcgtcc tctgcatcct
    16201 cgagaacgtc cggacgacgg tcgagaacca ggtcgaggcg gggctcgttg cacgtcttgc
    16261 ctgcacgctc cgggagaggc ttctcgatcc ggcctcgggg aacccgtacc cggcgacggc
    16321 ggacggcgac gccctcttct ggcggcgcgg gctcttcgtc gtcagcccgc accatgccca
    16381 gatcggcgcc atccgcgact gcctctcccg ggagagggcc tgggagtccc ggccgttcgt
    16441 ggataccgtc gataagatgc agggccagga ggcggaggcg gtcatcgtca gttacggcgt
    16501 aagcgacgtc gagaccgctc tctcggaggc cgggttcatc tacagcagga accgcctgaa
    16561 cgtctcgctg acccggggcc ggtcgaagtg cgtggtcttc ctgccccggc cgctgctcga
    16621 gccgccgctc gaccttgtcg gggacccgac ggcggcggcg gggctccggc acatgctcga
    16681 cctccaggag ttctgccgga cccacggcga ggagaagacg ttctccgggg atgcgcgagg
    16741 ggtccggctc acggtgatgc gggcgcgggt ggagtgaacc ggcccaacct atatccccgt
    16801 cccgaccgaa acgttgggtg tgatcggcag tctcgatccc gaccagaacg agcgcgaaca
    16861 gcggatcctc aaagaaaccc gcgagcacgg cgccaccccg gaggccgtcc gcctctttcg
    16921 cgctctcgtc ctcgcgcact accgcgccca cggccgcgac cttccctggc ggcagaccac
    16981 cgacccctac cggatactcg tctccgagat catgctccag cagacgcagg tcgagcgggt
    17041 cgccgtgaag tacccggagt tcatcgaccg gttccccgac tttgcaagcc ttgcccgcgc
    17101 tccgcagagc gatatcctcc ttgcctggca ggggatgggc tacaaccgcc gggcgctcgc
    17161 cctcaagagg accgcagagc gggtggtcga cgactacggc ggccggctcc cggccgacgt
    17221 cgagactctc gcgaccttcc ccggcatagg gagggcgaca gcggcggcca tatgcgcgta
    17281 tgccttcaac atgccggtcg cctacatcga gacgaacatc cggcgggtct tcatccactt
    17341 cttcttccag gaccgcgagg atgtcaggga cgacgagatc ctggtgctcg tcgagcagac
    17401 gctcgaccgc gagaatccgc gggagtggta cggtgcgttg atggattacg gctcgatctt
    17461 aaagaagcgg acggcaaacc cgaaccggcg gagcgcgtcc tactcccggc agagccggtt
    17521 cgagggctcc gaccggcaga tcaggggaag gctgctcgct ctcgtcctcg cggagggcgc
//
LOCUS       DAOSII010000031        17479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_3464_length_17479_cov_51.334826, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000031 DAOSII010000000
VERSION     DAOSII010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 17479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17479
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3464_length_17479_cov_51.334826"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..112)
                     /locus_tag="PLV90_03855"
     CDS             complement(<1..112)
                     /locus_tag="PLV90_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HOF96278.1"
                     /translation="MKAPKKPRSSSLSTAQQIYFGRVSGCHINPAVTIALF"
     gene            complement(170..1171)
                     /gene="fen"
                     /locus_tag="PLV90_03860"
     CDS             complement(170..1171)
                     /gene="fen"
                     /locus_tag="PLV90_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease-1"
                     /protein_id="HOF96279.1"
                     /translation="MGVAIRDILADCKEPLAWNDLSGIAAVDAHNALYQFLSIIRQPD
                     GTPLMNGAGRVTSHLSGILFRTVNFLEKGIRPVFVFDGRPPEFKQGTINERREIRARA
                     DEAWKTALAEGDMEEAYKQASASARIDGHILESSRELLDLLGIPRVQAPSEGEAQAAH
                     MARRAGVAYAVSQDYDSLLFGTPVLVRNLTISGRRKMRGRTITVNPERIVLASLLDRL
                     GVTREQLVDIGILVGTDFNPGIRGVGGKTALKIVRKGEFASVIAEKQPDFDPDPIREF
                     FLDPPVTDDYTLEWRPPDVEGVVSMLCGRYDFSEDRVRSALARAPAKGTQKTLDAWF"
     gene            complement(1220..1534)
                     /locus_tag="PLV90_03865"
     CDS             complement(1220..1534)
                     /locus_tag="PLV90_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96280.1"
                     /translation="MEIRELIGDIVLTIVMVVSTVVLVMRFWQDLTIAVAATLMMLSL
                     GGLLISLGMKIVSLERSVAQRERTMRVNIEEMEKTMSARYDNTISHIEGIVDGLSRRM
                     YR"
     gene            1592..2503
                     /locus_tag="PLV90_03870"
     CDS             1592..2503
                     /locus_tag="PLV90_03870"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042500 - aspartic endopeptidase activity,
                     intramembrane cleaving [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presenilin family intramembrane aspartyl
                     protease PSH"
                     /protein_id="HOF96281.1"
                     /translation="MQVRDWLSLLGMPLMLLSVQVIAVVLVLPMQAAGLAAFEDPESV
                     ANPLIFIGMLLVFTLILLLLIRFGGRRFIALFIGFAIFMTFLYIFSALSLLVLGATDA
                     AAISTLIAAAAATALIYLYPEWYVIDILGVLISAGVAAIFGVSLAILPVLVLLVLLAI
                     YDAISVYRTKHMITLAEGVLETKAPIMVVVPKRADYSFRREGLNISDGEERGAFVMGM
                     GDLIMPTILVTSSHVFVDAPAVLWTLSAPTLGAMAGSLAGLAVLLRFVNRGNPQAGLP
                     PLNGGAILGFLVGAVLAGSFGWCPFCI"
     gene            complement(2436..3416)
                     /locus_tag="PLV90_03875"
     CDS             complement(2436..3416)
                     /locus_tag="PLV90_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOF96282.1"
                     /translation="MEFETIPTVPTADEVLDRSLRRAAAKKKQKINVDRANEEFVRAV
                     ASAIHDKLKSVVASFPSFERLPPFYQEAADILVSLDRLKKSLGAVTWAADQVRTIGSG
                     YARSMRSAEETDRVRRQAVARIASVVHQVEDDLLFLNEARNILRKLPHVNEGEFTVVV
                     AGYPNVGKSSFIRLVSTAEPEIAAYPFTTKGVIVGHRDIGRRERIQFIDTPGVLERPA
                     DERNPIERQAVSAIINTADVVLFILDASEHCGYTLDDQVRLLEEVRGFVDVPVVAVAN
                     KADIRGIEGCPAMSTLTGEGVEAALDLLLIYRKDTTQTNLREPPQPGTQE"
     gene            3465..4343
                     /locus_tag="PLV90_03880"
     CDS             3465..4343
                     /locus_tag="PLV90_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOF96283.1"
                     /translation="MDLSPIQKDILITLITLYHQSSHSIKGEEIADVLKRNPGTVRNQ
                     MQALKALGLVDGVPGPKGGYSPTARAYKELNLADLEHQSEVPIFRDGEKVKGVRVSEI
                     GFTTLCHPDLCHAMVKIIGSVKLFKVGEMLSIGPTPVNKLLIRGEVFGMDENQQALMI
                     SISEMISLPKQAIKHYMSTPLLTLPLSATLRDAVHLFNTHHIHGAPVMEDGNLAGIVT
                     LSDIARALDQGQTLDAPVSGVMTTDVIEAPSSIRLYELVGRFKEREIGRLIVVEGGKP
                     VGIVTQTDIIRVFPSL"
     gene            4488..4697
                     /locus_tag="PLV90_03885"
     CDS             4488..4697
                     /locus_tag="PLV90_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone family protein"
                     /protein_id="HOF96284.1"
                     /translation="MVDIPLAPVGRIVKKSGAERVSSDANEELAKLMEQYASRIAKEA
                     IKLAGHAGRKTVKATDVRMAAETVK"
     gene            5154..5411
                     /locus_tag="PLV90_03890"
     CDS             5154..5411
                     /locus_tag="PLV90_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOF96285.1"
                     /translation="MSKTFCRTLAKKKVMSNDGMLIGTIKNIMVDLTTGQVVDLIVKP
                     DDTFKTDGYKMDGDKMVVPFEAVKDIKDYIVVDRYLLKRSG"
     gene            complement(5387..6091)
                     /locus_tag="PLV90_03895"
     CDS             complement(5387..6091)
                     /locus_tag="PLV90_03895"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HOF96286.1"
                     /translation="MLKALVTDVDGTLTDRRRRINTAAVEAIRTLVDAGIEVVLASGN
                     TVCFMDGLCRMIGTDGTIIGENGGVCRRGYKGSLHIAGDREICLEAFEVLHDHFAGQG
                     IDLELLSAQYRFADVALARNIDPDEARAVLRDHHLPVRVLDTGFAVHIQAPGVSKETA
                     MRKLAVDMGLSLDDMIAVGDSENDIEMLRAAGVGVAVQNAPELTRSAADWVSESAYGD
                     GFVEAVKKYYPDLFSR"
     gene            complement(6085..7059)
                     /gene="radA"
                     /locus_tag="PLV90_03900"
     CDS             complement(6085..7059)
                     /gene="radA"
                     /locus_tag="PLV90_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadA"
                     /protein_id="HOF96287.1"
                     /translation="MTEIDLEDLPGVGPTTAEKLREAGYGTVESIATATTSDLAEAAE
                     IGEGTAKKVILAARKMADIGGFKTGRDILDKRKDVKKLSTLVPEFDELVGGGLETQAI
                     TEVYGEFGSGKSQLVHQLAVNAQLPEDLGGLHGSIIYVDTENTFRPERIEQMVNGLPE
                     GTDLGSMEEILERIHVARAHSSDHQMLLLETARELANQLRTSDYPVRLFVIDSLTSLF
                     RSEYAGRGTLAARQQKLNRHMHDLLKLLDDHNAVGLVTNQVMSNPAILFGDPTKPIGG
                     NIVGHTATFRLYLRKSKGGKRVARLVDSPNLPEGEAPFMVEQAGLKPC"
     gene            complement(7167..8453)
                     /locus_tag="PLV90_03905"
     CDS             complement(7167..8453)
                     /locus_tag="PLV90_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HOF96288.1"
                     /translation="MKFHYLLVDDLVGREEFERRVDAKVAESGDLLDERTAAMLVVRD
                     LDRAHVRIRDLARTSSLACFFAKVLSVEAPREFERSDGTTGIVANVMVGDETGRAKLA
                     LWDEKAGGVSEIGVGSVLEILGRPKGGGGRVVDVTAIAIQEAACDITCNEADTLAPAG
                     PAGDIEVRLIAVEAPRAFRRRDGSPGEMVEAVVGNEKGIFRLVAWVPETLLEAETGTN
                     VVIRGAVARESDRGIEYSLGEAGSVSPSDREIVIPMDTIAGIEEGKSYSIAGTVVSVQ
                     PSRSFVTKGGRPSSVRNLVITDSTGEVPVVIWGEKADGHLVSGDRIEVYNAAARRGRY
                     GDTELHLSWGSALVVLAGEEEEVDVRGTVIATGQGVALDTGDACYLLADPLPVGYDLR
                     VRGSLHRGVITVHHAEAVIPDPGDLQSRLDRFSGQP"
     gene            complement(8470..9762)
                     /gene="lysA"
                     /locus_tag="PLV90_03910"
     CDS             complement(8470..9762)
                     /gene="lysA"
                     /locus_tag="PLV90_03910"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOF96289.1"
                     /translation="MILPSHLSVRGGHLYIGEHDTVALAERFGTPLYVTSEDRVRENF
                     RRLSGALSAHYGKVRVLYAAKANGNLAVFRTLASMGAGSDVFSAGEVALALAAGMRPE
                     LLLFNGSSKSPADLALAVEKGIRVSVDSPDELRQLDRAAGAAGRTVDIAFRVNPAIDV
                     PTHPKIATGLATSKFGIPAEEILDAYREALAAEHVNPVGVHCHIGSQILAVEPFAREV
                     EVLIDLAADLLDIGVDLSFIDIGGGLGIPYRRETDRAPTPEEYAGAVMPVFLEGIRDL
                     GIEPELWVEPGRWLVADSTVLLSRVNSVKTAHKTFANVDAGFNLLIRPTMYDSYHEVV
                     AANRADAPAVREYSIAGPICETGDVLAKDRMLPELAAGDIVAMLDAGAYGFAMSSQYN
                     SRPRCAEVLLAGEKAALMRRAETIDDLTAPMATPPWQE"
     gene            complement(9759..10907)
                     /locus_tag="PLV90_03915"
     CDS             complement(9759..10907)
                     /locus_tag="PLV90_03915"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOF96290.1"
                     /translation="MYARRMDHLPPYLFARIDDMKEAKQRQGVDVIDLGVGDPDLPTP
                     PHIVEALCTAARDPKNHHYPSYAGMPAYREAVAEWYRGRFGVDLDQKKEVLALIGSKE
                     GIAHIAEAFVNPGEVVLAADPGYPVYKTSTLFAEGKVHELPIRAENDFLPVLEDIPAD
                     VVKQAKLIFINYPNNPTAATAPLSFFEEVVEFAREHNIVVVHDNAYSEITFDGYKAPS
                     FLEVDGAMDVGIEMHSLSKTYNMTGWRIGMACGNPDIVAGLGRVKTNVDSGAFNAIQH
                     AAITALTGPQDCVEQACAIYQERRDALVKGLAELGLDVTAPKATFYVWAPVDDCMAFA
                     AKLLDETGIVATPGVGFGRNGEGFVRFAITRSIERIHEAVDRMRGIDL"
     gene            11016..12215
                     /gene="nifS"
                     /locus_tag="PLV90_03920"
     CDS             11016..12215
                     /gene="nifS"
                     /locus_tag="PLV90_03920"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOF96291.1"
                     /translation="MNDQAERPVYMDHAATTCMKPEVLAAMAPYFSEHFGNPSSLYRF
                     SRGSREGVEGARGEVAKALGAKPEEVFFTAGGTESDNWAIKGVAAAAKKKGGHIVTSA
                     IEHHAVLHTCEWLTKQGFSVTYLPVDGYGRVDPGDVENAITDRTVLVSVMTANNEIGT
                     VQPIGEIGRIAHDHGVLFHTDAVQAIGAVPIDVDAMGIDLLSLSGHKFYGPKGTGALY
                     IRQGTRIESLIHGGGQERGRRAGTENVPGIVGLGRAIEVATADIEGHNRRIAAMRDRL
                     IRGVLDTIPDTRLNGHPVERLANNANFSFRYVEGESILLLLDAHGICASTGSACSSGS
                     PEPSHVLLATGLTPEEAHGSLRLTLGDANTADDIDYVLRLLPEAIGRLRRISPLTPAE
                     KRVQEAD"
     gene            12215..12643
                     /locus_tag="PLV90_03925"
     CDS             12215..12643
                     /locus_tag="PLV90_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOF96292.1"
                     /translation="MYTEKVIAEFTNPEYVGEIADADGVGEVENPGCGDIMRISLKVE
                     GDRIVDARFKTFGCAAAIASSSMATRMIRGMTLSEAWNLSNADVVAALGGLPEPKVHC
                     SLLARGAIRAAINDYRTRRGLPPLEAGPSGCCEGTKPDNG"
     gene            12669..12986
                     /gene="cutA"
                     /locus_tag="PLV90_03930"
     CDS             12669..12986
                     /gene="cutA"
                     /locus_tag="PLV90_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOF96293.1"
                     /translation="MDLPEYVVVFCTAPAGEAEALARTLVDARLAACVNVTDVRSCYR
                     WEGKVHGEPEQLLIVKTQYRLLDPLVARIKALHSYETPEIIAMPIAGGYAPYLEWIRE
                     ETT"
     gene            12986..13735
                     /gene="fdhD"
                     /locus_tag="PLV90_03935"
     CDS             12986..13735
                     /gene="fdhD"
                     /locus_tag="PLV90_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HOF96294.1"
                     /translation="MEIVCRSGIQVTGEDVCAVRDDLIVEDRIRLFLNGEYLTTLVAS
                     PDRLDDLAAGFIICEGLADRVASVNVSGKDVYVTAPVGRDIRLEVESSGGYRVLGEPR
                     KVTSSITTTAETIRAVTAAIESDTWRRTGGVHCSVLFCGGKLATRACDVGRHNTVDKV
                     VGYAALRGLDRSTCILGCTGRQPAGMVAKAANAGIPIVVSRAASTDRGILTAERAGLT
                     LVCFSRGERFTIYTHPGRVSDVLAAIKKAQP"
     gene            13732..14205
                     /locus_tag="PLV90_03940"
     CDS             13732..14205
                     /locus_tag="PLV90_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1890 domain-containing protein"
                     /protein_id="HOF96295.1"
                     /translation="MTENDSRDALILLGCPQVPVQTSMALYLVHNLAQRGIRPVIAGT
                     PAARKLMEVADPGRHYLEEMTDIDAAIDEITEKRRDFDLCFVFIHNDSGVAYAGTMAY
                     ISEARVYALIFGEHAEDLAGEIEFPCEVVAAKATHNPMPLKRRLDEVMQWAVLRR"
     gene            14184..14456
                     /locus_tag="PLV90_03945"
     CDS             14184..14456
                     /locus_tag="PLV90_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1894 domain-containing protein"
                     /protein_id="HOF96296.1"
                     /translation="MGCLEALKPEILLSQTTFRDAREYIRKNIREYHEVEPGYRIFDV
                     HIIGVPPLYIGIEGGDLIFPYTKPCHGTFVVKVPGGEEIARLRARK"
     gene            complement(14509..15066)
                     /locus_tag="PLV90_03950"
     CDS             complement(14509..15066)
                     /locus_tag="PLV90_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOF96297.1"
                     /translation="MHTSSRSFLCICALGLLLITAGCTQNEPDTIVSAPETPGIRGPE
                     HYTANETLVAFVESAVDYARTHGKDKALVEFSDPNGSFVRGELYIYAYDFNNTVLAHP
                     FDPDKIGVNRMDELDAFGNPYTQQFLDAAKNGSGFVRFYYVNPAHNRTVESKLGYVMK
                     VDEDWWLGSGIYMGPADTGASRATP"
     gene            15369..>17479
                     /locus_tag="PLV90_03955"
     CDS             15369..>17479
                     /locus_tag="PLV90_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF96298.1"
                     /translation="MSEPLRVLLIGESPGNLGEMLRTCGRKAELIHRERPRDGLALAS
                     GGEIDVVLLDLDLADGEGTAFLDRLRRTAPDIPVIVLTAAGEDRAAFHAMQSGAQDYL
                     VREKTDAELLCRAIRYAQERMRAEVLLRHSEAMYRRLIENLNEGIIAVDEAGLITFAN
                     PRMGEIFGCPARRLVGLPIAGFIAGENIPAAPNPFCRQTDQRQEFEQDLIRCDGGQVH
                     ALVATSPIVDAAGAPLGCLAGVLDITGRKQAEEALRQSEQRYRLVVESLSEGIWVIDR
                     NACTSFVNPRMAEMLGYSPGEMIGRSVHSFLPASGWAAMQDRLKRRRDGTREQYECKF
                     TRKGGETITVLVISSPFVDDAGEFQGSIVGVMDITERKRVEEEIRVRNEQLMVLNQVI
                     SVSATSLSLAELLEESLEKTLNLMGFHVGIVYMLDAERKQATLQHQRGMPPSCMASNR
                     IIKVHHWPFNFTFIAGQPRYIERQADLSSIEATILEEFGVSALACIPLIAESVVVGAV
                     YAGSREKMSFSREERTLLESIGKEIGSGILKGMLHKRLEAANREANLYLDIMTHDIKN
                     AENVSNLYTDLLIGMLEGETAQYVRRIQSSIQKSAGILRNVTTIRRIHHDASDLGPID
                     LDAVIRGEIEAFPGIDIEFSGIRCPVWADDLLCEVFSNLIRSAVRFGGSDIRIVIRVE
                     DYDGENVLVSIEDTGPGIPDSV"
BASE COUNT         3114 a         5452 c         5641 g         3272 t
ORIGIN      
        1 caaagagggc tatcgtcacc gccgggttga tgtgacagcc cgataccctt ccgaaatata
       61 tctgttgcgc cgttgacaga gaggaggacc ggggtttttt tggggctttc atccccaatg
      121 atgctcgata cccgcgattt tggtgggaaa cgatctctcc ggcgcctcgt cagaaccagg
      181 catcgagcgt cttctgcgtc cccttcgccg gggccctggc aagagcgctc ctcacccggt
      241 cttcggagaa gtcgtagcgt ccacagagca tggagacgac cccctcgacg tccggcggcc
      301 gccactcgag ggtgtagtcg tcggtgaccg gcggatcgag gaagaactcg cggatcggat
      361 cagggtcgaa gtcgggctgt ttctcggcga tgacagatgc aaattcacct tttcgaacga
      421 tcttcagggc cgtcttgccg ccgacgcccc ggatgccggg gttgaagtcc gtgcccacca
      481 ggatgccgat atcgacgagt tgctccctcg tcacgccgag gcggtcgagg agagaggcga
      541 ggacgatgcg ctcgggattc accgtgatcg tccgtccccg catcttccgt ctcccgctga
      601 tcgtgaggtt ccgcaccagg accggggtgc cgaagaggag cgagtcgtag tcctgcgaga
      661 ccgcgtaggc gacgcccgcc ctccgcgcca tgtgggcggc ctgcgcttcg ccttcgctcg
      721 gcgcctgcac ccgggggata ccgagcaggt ccaggagctc gcgggacgat tcaagtatat
      781 ggccgtcgat ccgggcggat gcgctcgcct gtttgtacgc ctcctccata tccccctccg
      841 caagcgcagt cttccaggcc tcgtcggccc ttgcgcggat ctcgcgccgc tcgttgatcg
      901 tcccctgctt gaactccggc ggtcttccgt caaagacaaa gaccggccgg atccccttct
      961 ccaggaagtt caccgtccgg aagaggatgc cggagaggtg cgaggtgacc ctccctgcac
     1021 cgttcatcag cggggtgccg tcaggctgcc ggatgatgga gagaaactgg tagagcgcgt
     1081 tgtgggcatc cacggcggcg atgccggaga ggtcgttcca cgccagcggt tctttacagt
     1141 ctgcgagaat atcgcgaata gcaacgccca tgctatctac tcatgagaga gggagatacc
     1201 attaagatgg cggtccggtt cagcggtaca tccggcggga gagcccgtcg acgatcccct
     1261 cgatgtggct gatcgtgttg tcatacctgg cgctcatcgt cttctccatc tcctcgatgt
     1321 tcacgcgcat ggtgcgctcc cgctgtgcga cgctccgctc gaggctcacg atcttcatgc
     1381 cgagagatat gagcaagccc ccaagagaga gcatcatgag cgtcgcagca accgctatgg
     1441 tcaggtcctg ccagaaccgc atcaccagca cgacggtgga gaccaccatc acgatggtga
     1501 gaacaatgtc gccgatgagt tcgcgaatct ccatatttta aagtgatgag gatgcattaa
     1561 ttaacctacc taaaatgact ggagacatat gatgcaggta cgtgactggc tatcgcttct
     1621 tggaatgccc cttatgctcc tctccgtcca ggtgatcgcc gtcgtcctcg tgcttcccat
     1681 gcaggcggcg ggtcttgcgg cgttcgaaga tcctgaatcg gttgcaaatc ccctgatatt
     1741 tatagggatg ctgctcgtct tcacgctgat cctgctcctc ctgatacggt tcggaggccg
     1801 gcgcttcatc gccctcttca tcgggtttgc catattcatg acattcctct atatcttctc
     1861 ggcgctctcg cttcttgtgc tcggggccac cgatgccgct gccatcagca cgctcatcgc
     1921 tgccgcggcg gcgacggcgc tcatctatct ctaccccgag tggtacgtca tcgatatcct
     1981 cggcgtcctg atatccgcgg gggtcgcggc gatcttcggg gtctcccttg ccatcctccc
     2041 ggtccttgta ctgctcgttc tgctcgctat ctatgatgcc atatcggtct accgcacgaa
     2101 acacatgatc acgcttgcgg agggcgttct cgagacgaaa gccccgatca tggtcgtggt
     2161 cccgaaacgg gcggattact cgttccgaag agaggggctc aatatcagtg atggagaaga
     2221 gcgcggcgcg ttcgtcatgg gcatgggcga cctcatcatg cccaccatcc tcgtcacgtc
     2281 gtcgcacgtc ttcgtggatg cacccgccgt cctctggacc ctctccgccc cgacgctcgg
     2341 cgcgatggcg ggatcgcttg ccggccttgc cgtcctcctc cgcttcgtca acaggggcaa
     2401 cccccaggca ggcctcccgc ccttgaacgg cggcgctatt cttgggttcc tggttggggc
     2461 ggttctcgca ggttcgtttg ggtggtgtcc tttctgtata tgaggaggag atcgagcgcc
     2521 gcctccacgc cctcgccggt gagcgtggac atcgcggggc agccttcgat cccccggata
     2581 tccgccttgt ttgcgaccgc caccaccggg acatcgacaa acccgcggac ctcttcgagg
     2641 agccggacct gatcgtcgag cgtgtagccg cagtgctcgc ttgcatccag tatgaagagc
     2701 acgacgtcgg ccgtgttgat gatggcgctc accgcctgcc gctcgatggg gttcctctcg
     2761 tcggccgggc gttcgaggac accgggcgta tcgatgaact ggatccgttc gcgccgcccg
     2821 atatcgcggt ggccgacgat gacccctttg gtggtgaaag ggtaagcggc gatctcgggt
     2881 tccgccgtgg agacgagcct gatgaacgat gatttcccga cgttggggta tcctgccacg
     2941 accacggtga actcgccctc gttcacgtgc ggaagttttc ggagtatgtt tcgcgcttcg
     3001 ttcaggaaga ggaggtcatc ctccacctgg tggacgacgg aggcgatgcg ggcgacggcc
     3061 tgccgccgga cccggtcggt ctcctctgcg gatcgcatgc tgcgggcgta gcccgaaccg
     3121 atggtccgga cctggtcggc cgcccaggtg acggcgccga gagacttctt caaccggtcg
     3181 agcgagacga gtatgtcggc cgcctcctgg taaaagggcg ggaggcgctc gaagctcggg
     3241 aacgacgcga ccacgctctt gagtttatca tggatggcgc tcgcgaccgc tcttacgaat
     3301 tcctcgttcg cacggtcgac gtttatcttt tgcttctttt tggctgccgc ccggcgaaga
     3361 ctgcggtcga gcacctcatc tgccgtcggg acggtcggta tagtttcaaa ctccactgca
     3421 cgcacaacct aatctattta aacaacgcat tatgagtgat aattatggat ctttccccaa
     3481 ttcagaagga tatcctgata accctgatta ccctgtatca tcagtcttct cactctataa
     3541 aaggtgagga gatcgcggat gtgctgaagc gcaaccccgg caccgtccgc aaccagatgc
     3601 aggcattaaa agcgctcggg cttgtagacg gcgttccggg cccgaaaggc ggttacagcc
     3661 cgaccgcacg ggcatacaag gagctcaatc ttgccgatct cgaacaccag tccgaggtgc
     3721 cgatctttcg cgacggcgag aaggtgaaag gtgtccgggt ctcggagatc gggttcacca
     3781 cgctctgcca tcccgacctc tgtcatgcga tggtcaagat catcgggagc gtgaagctct
     3841 tcaaggtagg cgagatgctc tcgatcggac ccacgccggt gaacaaactc ctcatccgcg
     3901 gcgaggtctt cggaatggac gagaaccagc aggccctcat gatcagcata tcggagatga
     3961 tatcgctccc gaagcaggcg atcaagcact atatgagcac cccgctcctg accctgccgc
     4021 tctccgccac cctccgtgat gccgttcatc tcttcaacac gcaccatatt cacggggcgc
     4081 cggtgatgga ggacggcaac cttgcaggca tcgtgacgct gagcgatatc gcccgcgccc
     4141 tggaccaggg ccagacactg gatgcgcccg tttccggggt catgaccacc gacgtgatag
     4201 aggcgccgtc gtcaattcgg ctctacgagc tcgtgggccg gtttaaggaa cgggagatag
     4261 ggaggttgat tgtagtggag ggcggcaagc ccgtcggtat cgttacccag accgatatta
     4321 ttcgggtctt tccctcactt tgataccctc cccgttccga atttcctgct tcaacccctc
     4381 tggaagggga taagtattta tatatgaata cctatggtgc ttctgtaaaa cgagctctgt
     4441 ttgagagtac atttcgcaga gctcaatgaa aagggggaaa tgagatcatg gtagatatac
     4501 ctctggcacc tgttggcaga atcgtgaaga agtccggcgc ggaacgagtg agttctgatg
     4561 ccaatgaaga gctcgcaaaa ctcatggagc agtacgcatc gcgaatcgca aaagaggcga
     4621 tcaagctcgc gggacacgca ggccgaaaga cggtcaaggc aaccgatgtc cggatggcgg
     4681 ccgagaccgt aaagtaaacc aatcccgact ccatttttcc ccgggacaac ctgttgtgcc
     4741 ggttattatt gctgcaagaa ctgctctacc tgccggggtg cccgtctttg cccctgcagg
     4801 gatacccgta cgccgttctt cgggaactcc caccccttcc cgggcggggc agcgttcgcg
     4861 atcattcgat gggaagccct gcccggtcct tctccgggga tctccccgtg gagatatcgc
     4921 ggaggggcat gaacctcacc cctgcggggc tcaaggttac cgtcatgccg gccctcacga
     4981 acggttgcgg caccgcacct ccgggctgag agcgggggga cggagaccgg gactgccggg
     5041 gggcagggcg gttgtggtgg ccggcctctc cgggcagcga tcctggaaag ccatttctga
     5101 agagttttga gggatttaga atatttatat agtacaaaca ccccacgctc tctatgagca
     5161 aaaccttctg ccgcaccctt gccaagaaga aagtgatgag taatgacggc atgctcatcg
     5221 ggaccatcaa gaatatcatg gtcgacctca ctacggggca ggtcgtcgac ctgatcgtca
     5281 agccggacga tacgttcaag accgatggct acaagatgga tggagataag atggtcgtgc
     5341 cgtttgaggc ggtgaaagac ataaaagatt atatcgtggt cgaccgctac ctgctgaaga
     5401 gatcggggta atactttttt acggcttcca caaacccatc cccgtatgcg ctctccgaga
     5461 cccagtccgc cgccgaccgg gtcagttccg gcgcgttctg caccgcgacg ccaaccccgg
     5521 cagccctgag catctcgata tcgttctcgg aatccccgac ggctatcata tcatcaaggg
     5581 atagtcccat atccacggcg agttttcgca tcgccgtctc cttgctcacg ccgggtgcct
     5641 ggatgtggac ggcaaacccc gtgtccagca cccggacagg aagatggtgg tcacggagga
     5701 ccgcccgtgc ctcgtcgggg tcgatgttcc gggcaagggc cacgtcggca aaccggtact
     5761 gtgcgctgag cagctcgaga tcgatgccct gcccggcaaa atgatcgtgt aagacctcga
     5821 acgcctcaag gcagatctcc cggtcgccgg cgatatggag ggagccttta taccccctcc
     5881 ggcagacgcc gccgttctcg ccgatgatgg tcccgtccgt cccgatcatc ctgcagagcc
     5941 cgtccatgaa acagacggtg ttgccgcttg caagcaccac ctcgataccc gcatcgacga
     6001 gcgtccggat ggcctcgacg gcagcggtgt tgatccgccg ccgccggtcg gtgagggtgc
     6061 cgtcgacatc cgttacgagt gccttcagca cggcttgagc cccgcctgct cgaccatgaa
     6121 cggcgcctcg ccctcgggga ggttggggct gtccacaagg cgggcaaccc tcttgccgcc
     6181 cttgctcttg cgcaggtaga ggcggaaggt tgcggtgtgc ccgacgatgt tgccgccgat
     6241 cggttttgtc gggtcgccaa agaggatggc agggttcgac atcacctggt tggtcacgag
     6301 accgaccgcg ttgtggtcgt cgagcagctt caagaggtcg tgcatgtgcc ggttcagttt
     6361 ctgctgacgg gcggcgaggg tcccgcgccc tgcgtactcc gagcggaaga gggacgtcag
     6421 cgagtcgatg acgaagagcc tgaccggata gtcggatgtc cgcaactggt ttgcgagttc
     6481 ccgtgcggtc tcgaggagca gcatctggtg gtcggagctg tgtgccctgg cgacgtggat
     6541 acgctcgagg atctcctcca tcgaaccgag gtccgtaccc tccgggaggc cgttgaccat
     6601 ctgctcgata cgctccgggc ggaacgtgtt ctcggtgtcg acgtatatga tgctcccgtg
     6661 cagcccgcca aggtcttcgg ggagctgggc gttgacggcc agctggtgga cgagctggct
     6721 cttcccggac ccgaactccc cgtacacctc ggttatcgcc tgcgtctcaa gacccccgcc
     6781 gaccagttcg tcgaactcgg ggaccagggt gctgagtttc ttgacgtctt ttctcttgtc
     6841 cagtatatcc cggcccgtct tgaacccgcc gatatccgcc atcttccgtg cggcgaggat
     6901 caccttcttc gcggtcccct cgccgatctc ggccgcctcg gcgagatccg acgtggtggc
     6961 cgttgcaatg ctctcgacgg tcccgtagcc cgcctcgcgg agtttttcgg cggtggtcgg
     7021 cccgacgccc ggtaaatctt caagatcaat ctctgtcatg tcaatcctca cttgatctct
     7081 gtggttgttt gttatgttac acttagagtg ttgaacggga gacataaagg tcgcttgcca
     7141 tgcggggtga aagtgaaagt cagggatcag ggttgcccgg agaaccggtc caggcggctc
     7201 tgcagatctc ccggatcggg gatcacagcc tcggcgtggt ggacggtgat cacgccccgg
     7261 tgcaggctac cccggacgcg cagatcgtac ccgaccggca acgggtcggc aaggaggtag
     7321 caggcgtcgc cggtatcaag agcgaccccc tgcccggtgg cgatgaccgt ccccctgaca
     7381 tccacctcct cctcttcccc ggcaaggacg acgagggcgc ttccccagga gaggtgcagt
     7441 tcggtatcgc cataccgccc ccggcgggcg gccgcgttgt agacctcgat acggtcgccg
     7501 gataccaggt gcccgtccgc tttctccccc catatgacga ccgggacctc gccggtcgaa
     7561 tcggtgatga ccaggttccg gacggacgac ggcctcccgc ccttcgtgac gaacgaccgg
     7621 gacggctgga cggatacgac cgtcccggcg atcgagtacg acttaccttc ctcgatgccc
     7681 gcgatcgtat ccatcgggat gacgatctcc cgatcggagg gcgagacgga tcctgcctcg
     7741 ccgaggctgt actcgatgcc gcggtcgctc tcccgcgcga cggcgccccg gatgacgacg
     7801 ttcgttcccg tttccgcctc aagaagggtc tccgggaccc aggcgacgag ccggaagatg
     7861 cccttttcgt tcccgacgac cgcctcgacc atctcccccg ggctcccgtc gcgtctcctg
     7921 aacgcccgcg gggcttcgac ggcgatcagc ctgacctcga tgtcgcccgc cggcccggcg
     7981 ggcgcgaggg tgtcggcctc gttgcacgtg atgtcgcacg ctgcttcctg gatggcgatc
     8041 gcggtgacgt cgacgactct tcccccgccg cctttcggcc ggccgagtat ctcgagcacg
     8101 ctcccgacgc cgatctccga gacgccgccg gccttctcgt cccagagggc gagcttcgcc
     8161 cttccggtct cgtcgccgac catcacgttc gcgacgatgc ccgtcgtgcc gtcggaccgc
     8221 tcgaactccc ggggcgcctc gaccgagagg acctttgcaa agaagcacgc aaggcttgag
     8281 gtgcgggcaa ggtcgcgtat ccggacatgc gcccggtcga ggtctctgac gacgagcatc
     8341 gccgccgtcc gctcatcgag gaggtcgccg gactccgcca ccttcgcatc gacccggcgc
     8401 tcgaactcct ctctccccac gaggtcgtcg accaggaggt agtgaaactt cacggtcgcc
     8461 gctccggccc tactcctgcc agggcggcgt cgccatcggg gcggtcaggt cgtcgatcgt
     8521 ctcggcccgg cgcatcaggg ccgccttctc gcccgcgagg agcacctcgg cgcaccgggg
     8581 gcggctgttg tactgcgacg acatggcaaa cccgtatgcc ccggcatcca gcatcgcgac
     8641 gatgtcgccg gcggcaagtt cggggagcat cctgtctttt gcgaggacgt cgcccgtctc
     8701 gcagatcggc cccgcgatcg agtactcccg caccgcgggt gcgtccgccc tgtttgccgc
     8761 gacgacctcg tggtaggagt cgtacatcgt cgggcgaatg aggaggttga acccggcgtc
     8821 gacgtttgca aacgtcttat gggcggtctt gaccgagttg acccgggaga ggaggaccgt
     8881 cgagtcggcg acgagccacc ggccgggttc gacccagagt tcgggctcga tcccgaggtc
     8941 acggatgcct tcgaggaaga ccggcatcac cgctcccgcg tactcctccg gcgtcggggc
     9001 gcggtcggtc tcccgccggt aggggatgcc gaggccgccg ccgatgtcga tgaacgagag
     9061 gtcgaccccg atatcgagga gatcggccgc aagatcgatc agcacctcta cctcgcgggc
     9121 gaagggctcc acggcgagga tctgcgagcc gatgtggcag tggaccccga ccgggttcac
     9181 gtgctcggcg gcgagcgcct cgcggtaggc atcgaggatc tcctctgccg ggatgccgaa
     9241 cttgctcgtc gcaaggccgg tcgcgatctt cgggtgggtg gggacgtcga tcgcggggtt
     9301 gacccggaat gcgatgtcga cggttcttcc tgccgctccc gccgcgcggt cgagctgccg
     9361 gagctcgtcg ggcgagtcga ccgagacccg gatgcccttc tcgaccgcga gggcgaggtc
     9421 ggcgggggat ttcgagctcc cgttgaagag gagcaactcc gggcgcatac cggcggcaag
     9481 ggcgagcgcg acctcgccgg cggagaagac gtccgatccc gcgcccatag acgcgagtgt
     9541 ccggaatacc gcaaggttgc cgttcgcctt tgcggcgtag aggacgcgga cctttccgta
     9601 gtgggcgctg agcgctcccg agaggcgccg gaagttctcg cggacccggt cctcgctcgt
     9661 gacgtagagc ggggtgccga accgctccgc gagggccacc gtgtcgtgct cgcctatgta
     9721 gaggtgcccg cccctgaccg agaggtggga ggggaggatc acaggtcgat cccccgcatc
     9781 cggtcgaccg cctcgtggat ccgctcgatc gaccgggtga tggcgaaccg gacgaaccct
     9841 tcgccgttcc tgccgaagcc gacgccgggg gtcgcgacga tcccggtctc gtcgaggagt
     9901 ttcgcggcaa acgccatgca gtcgtcgacc ggcgcccaga cgtagaaggt ggccttcggc
     9961 gccgtgacgt caagcccgag ctcggcgagc cccttcacca gggcgtcgcg gcgctcctgg
    10021 tagatcgcgc acgcctgctc aacgcagtcc tgggggccgg tgagcgccgt tatcgccgcg
    10081 tgctggatgg cgttgaacgc gccggaatcg acgttcgtct tgacccggcc gagcccggcg
    10141 acgatgtcgg ggttgccgca ggccatgccg atccgccacc cggtcatgtt gtaggtcttg
    10201 gagagggagt gcatctcgat gccgacatcc atcgcgccgt cgacctcgag gaacgagggc
    10261 gccttgtagc cgtcgaaggt gatctcggag taggcgttgt cgtggacgac gacgatgttg
    10321 tgctcccgcg cgaactcgac gacctcttcg aagaacgaga ggggcgccgt cgccgcggtg
    10381 gggttgtttg ggtagttgat gaagatcagc ttcgcctgct tcacgacatc ggcggggata
    10441 tcctcgagga ccgggaggaa gtcgttttcg gcccgtatgg ggagttcgtg gactttaccc
    10501 tcggcaaaga gggtggatgt cttgtagacc gggtagccgg ggtcggcggc aaggacgacc
    10561 tcgcccgggt tgacgaaagc ctcggcgatg tgcgcgatgc cttcctttga cccgatgagg
    10621 gcgagcacct ccttcttctg gtccaggtcg acaccgaacc tgccccggta ccattcggcc
    10681 accgcctcgc ggtaggcggg cataccggcg taggaggggt agtggtggtt ttttgggtcg
    10741 cgcgccgcgg tgcagagcgc ttccacgatg tgcgggggtg tggggaggtc ggggtcgccg
    10801 acgccgaggt cgatgacgtc gactccctgg cgctgttttg cctctttcat atcgtcgatg
    10861 cgggcaaaga ggtagggggg aaggtggtcc atgcgtcttg cgtacattac cagatcttac
    10921 gcacggaacc ctatgtaatt atccgcgacc gggcctcccc gtggctcccg gccggcaacg
    10981 ctgatacgct tccagcgcgt acgctgtacc tggacatgaa cgaccaggca gagcgccccg
    11041 tgtacatgga tcacgcggcg acgacatgca tgaagcccga ggtcctcgcg gcgatggccc
    11101 cgtacttctc ggagcacttc ggcaacccct catccctcta ccggttctcc cgcgggtccc
    11161 gcgagggcgt ggagggggcg aggggagagg tggcgaaggc tctcggggca aagcccgagg
    11221 aagtcttctt caccgccggc ggcacggaat ccgacaactg ggcgatcaag ggggtggcgg
    11281 ccgccgcaaa aaagaagggc ggccatatcg tcacctccgc gatcgagcac cacgccgtcc
    11341 tccatacctg cgagtggctc acgaagcagg ggttctcggt cacctacctc ccggtcgacg
    11401 ggtacggccg ggtggaccct ggcgacgtcg agaacgcgat caccgaccgg acggtcctcg
    11461 tctcggtgat gacggcaaac aacgagatcg ggacggtcca gccgatcggg gagatcggcc
    11521 ggatagcgca cgaccacggc gtcctctttc ataccgacgc cgtccaggct atcggggcgg
    11581 tcccgatcga cgtggacgct atggggatcg acctcctctc gctctccggg cacaagttct
    11641 acggcccgaa ggggaccggg gcgctctaca tacggcaggg aacccgtatc gagagcctga
    11701 tccacggcgg agggcaggag aggggccggc gggccgggac cgagaacgtt cccgggatcg
    11761 tcgggctcgg gcgcgcgatc gaggttgcga ccgccgatat cgaggggcac aaccgccgga
    11821 tcgccgcgat gcgggaccgg ctgatccgcg gggtcctcga taccatcccg gatacccggc
    11881 tgaacggcca cccggtcgaa cggctcgcga acaatgcgaa cttcagtttc cgctacgtcg
    11941 agggggagtc tatcctgctc ctgctcgacg cccacggtat atgcgcatcg accgggagcg
    12001 cctgctcgtc ggggtcgcct gagccctcgc acgtcctgct cgcgacgggg cttacccccg
    12061 aagaggcgca cgggtcgctc cggctgacgc tcggcgatgc caatacggcg gatgatatcg
    12121 attatgtcct tcgtctgctg ccggaggcga tcgggcggct gcggcggata tcgccgctga
    12181 cgcctgcaga gaagagagtg caggaggccg actgatgtat acggagaaag tgattgcaga
    12241 gttcacgaac ccggagtacg tgggcgagat agcggatgcc gacggtgtcg gcgaggtcga
    12301 gaacccgggt tgcggggata tcatgcggat ctccctcaag gtcgagggcg accggatcgt
    12361 ggatgcccgg ttcaagacgt ttgggtgcgc agcggcgatc gcatcgagtt cgatggcgac
    12421 ccggatgatc cgggggatga ccctctccga ggcatggaac ctctcgaacg ccgacgtggt
    12481 ggctgctctc ggcggcctcc cggagccgaa ggtgcactgc tcgctccttg cccgcggcgc
    12541 catccgggca gcgatcaacg actaccggac ccgccgggga cttccccccc tggaggcggg
    12601 accctccggg tgctgcgagg gaacaaaacc cgataacggt taaatacagg cggtacgaag
    12661 ttaaaaccat ggatctcccg gaatatgttg tggtcttctg cacggcaccg gccggggagg
    12721 cggaagcgct tgcacgtacg ctcgtcgatg cccggctcgc ggcctgcgtg aacgtcaccg
    12781 atgtgcggtc ctgctaccgg tgggaaggta aggtccacgg cgagcccgag caactcctga
    12841 tcgtcaagac gcagtaccgc ctgctcgacc ccctcgtcgc ccggatcaag gcgctccaca
    12901 gttacgagac ccccgagatc atcgccatgc ccatcgccgg cggctacgct ccctacctcg
    12961 agtggatacg ggaggagacg acctgatgga gatcgtctgc cgctccggta tccaggtgac
    13021 gggcgaggat gtgtgcgccg tccgcgacga cctcatcgtc gaggaccgca tccggctctt
    13081 cctgaacggc gagtacctca cgaccctggt ggcgagtccc gaccgcctcg acgacctcgc
    13141 ggccgggttc atcatatgcg aggggctggc cgaccgggtc gcttcggtga acgtctccgg
    13201 aaaggatgtg tatgtcacgg ccccggtcgg gcgggacatc cggctcgagg tggagtcgtc
    13261 cggggggtac cgcgtgctcg gcgaaccgcg gaaggtcacc tcgtcgatca ccaccacggc
    13321 ggagaccatc cgggcggtca cggcggcgat cgagtccgat acctggcgga ggaccggcgg
    13381 cgtccactgt tcggtcctct tctgcggcgg caagcttgcc acccgggcct gcgatgtggg
    13441 gaggcacaac accgtcgata aggtcgtggg ctacgcggcc ctccgggggc tggaccggtc
    13501 gacctgcatc ctcgggtgca cggggaggca gccggcgggg atggtcgcga aggcggcgaa
    13561 cgcgggcatc ccgatcgtcg tctcccgcgc cgcctcgacc gaccggggca tcctcacggc
    13621 ggagcgggcg gggctcaccc tggtctgctt ctcgcggggg gagcggttca ccatctacac
    13681 gcaccctggg cgggtgtccg acgtccttgc cgcgataaag aaggctcaac catgacggag
    13741 aacgatagcc gggacgcgct gatcctgctc gggtgcccgc aggtgcccgt ccagacgagc
    13801 atggcgctct acctggtgca caacctcgcg cagcggggca tccggccggt gatcgccggg
    13861 acgccggctg cgcggaaact gatggaggtg gccgacccgg gccggcacta cctggaggag
    13921 atgaccgata tcgatgccgc gatcgatgag atcacggaaa aacggcggga cttcgatctc
    13981 tgcttcgtct tcatccacaa cgattcgggg gtcgcgtatg ccgggacgat ggcgtatata
    14041 tcagaggcgc gggtgtatgc gctcatcttc ggggagcatg cggaggacct tgccggcgag
    14101 attgagttcc cctgcgaggt cgtggcggcg aaggccaccc ataaccccat gccgctgaag
    14161 cggcgactcg acgaggtgat gcaatgggct gtcttgaggc gctgaaaccc gagatcctcc
    14221 tctcccagac gacgtttcgg gacgcgagag agtacatcag gaagaacata cgggagtacc
    14281 acgaggtcga accgggctac cggatattcg acgtccacat catcggcgtg ccgccgctgt
    14341 atatcggcat cgaaggcggg gacctgatct ttccctacac caagccctgc cacgggacgt
    14401 tcgtggtgaa ggtccccggc ggcgaggaga tcgcgaggct gcgggcgaga aaatagtact
    14461 tttctcttct atttcttcca aaaatgtgaa tgcggtccgg atggtcatct acggcgtagc
    14521 ccggctggct cccgtatctg ccggtcccat gtagatcccg gagccgagcc accagtcctc
    14581 gtccaccttc atcacgtagc ccagtttgga ctcgaccgtc cgattgtgcg cggggttgac
    14641 gtagtagaac cggacgaacc cggagccgtt ctttgccgca tcgaggaact gttgcgtgta
    14701 agggttgccg aaggcgtcga gttcgtccat ccggttgacg ccgatcttgt cggggtcgaa
    14761 cggatgggca aggacggtgt tgttgaagtc gtaggcgtag atgtagagct cgccccggac
    14821 gaacgaaccg ttcgggtcgg agaactcaac aagggcttta tccttgccgt gcgtcctggc
    14881 gtagtcgaca gcgctctcga cgaaggcgac gagcgtctcg tttgcggtgt agtgctccgg
    14941 tccccggatg ccgggagttt ccggcgccga aacgatcgtg tcgggctcgt tctgtgtaca
    15001 tcccgcggtg atgaggagca agccgagggc gcagatgcag agaaacgatc ttgaagacgt
    15061 atgcatatga ttgagtcggt atttattccc taaaaatgca tgccgaggac aaccggcgcc
    15121 ttatgggccc ttccggccgc gggactgggc gctgccggga gcccggcgat gggcactgcc
    15181 gccgccggag catctccggt gtgcgggaac ccatgcagga gagacgccca aagaaagagg
    15241 tataaattca ccgagggcgg tactacagta gtgagaaccc ggtcgcctgc ggtcatgccc
    15301 gcgatggccg ggagagcgaa gtttacgccg atacgggcag gtaccgggcc ctgaatgcga
    15361 gtgatacgat gagcgaaccg ttgagagtgc tgttgatcgg ggagagtccc ggcaatctcg
    15421 gggagatgct tcgcacctgc gggcgcaagg ctgagttgat ccaccgtgaa cgcccccggg
    15481 acgggcttgc cctggcatcg gggggcgaga tcgacgtcgt gctcctcgat ctcgatctcg
    15541 cggacggaga ggggaccgcc tttctcgacc gcctgcgccg gacggctccc gatatcccgg
    15601 tcatcgtgct cactgcggcg ggagaagacc gcgccgcgtt ccatgcgatg cagagcggcg
    15661 cgcaggatta cctggtccgg gagaagaccg acgcggaact gctctgccgg gcgatccggt
    15721 acgcgcagga gcggatgcgg gcggaggttt tgctccggca ctccgaggcg atgtaccgga
    15781 ggctgatcga gaacctcaac gagggcatca tcgctgtgga cgaggccggc ctcatcacct
    15841 tcgccaaccc gcgtatgggg gagatcttcg gctgccccgc aaggcgcctt gtcgggctgc
    15901 cgatcgccgg tttcatcgcc ggcgagaaca tcccggccgc cccaaatccg ttctgccggc
    15961 agaccgacca gagacaggag ttcgaacagg acctcatccg ctgtgacggc gggcaggtcc
    16021 acgcgctcgt ggccacgtcg ccgatcgtgg atgccgccgg cgcccctctg gggtgccttg
    16081 caggggtgct cgacatcacc ggccgcaagc aggccgagga ggccctcagg cagagcgagc
    16141 agaggtatcg actggtcgtg gagagcctca gcgaggggat atgggtgatc gaccggaacg
    16201 cctgcacgtc gtttgtcaac ccccggatgg cggagatgct cggctactcc ccgggagaga
    16261 tgatcgggag atccgtccat tcgttcctcc ccgcctccgg ctgggctgcg atgcaggacc
    16321 gcctgaagcg tcgacgggac ggaaccagag agcagtacga gtgcaagttc acccggaagg
    16381 ggggcgagac catcaccgtg ctggtgatat cgtcgccgtt cgtagacgac gccggggagt
    16441 tccagggctc gatcgtcggc gtgatggaca tcaccgaacg aaagagggtg gaagaggaga
    16501 tccgggtcag aaacgagcag ctcatggtcc tcaaccaggt gatcagcgtc tcggccacct
    16561 cgctctccct tgccgagctc ctggaggagt cgcttgagaa gacgctcaac ctcatggggt
    16621 ttcacgtcgg catcgtctac atgctcgacg ccgagcgcaa gcaggctacg ctgcagcacc
    16681 agagagggat gccgccatcg tgcatggcga gcaaccgtat catcaaggtc caccactggc
    16741 cgttcaactt caccttcatc gccggccaac cccgctacat tgaacgacag gccgacctga
    16801 gctccatcga ggcgaccata ctcgaggagt tcggcgtctc ggcgctcgcc tgcatccccc
    16861 tcatcgccga gtcggtcgtc gtcggggcgg tgtacgccgg cagcagggag aaaatgtcgt
    16921 tctcccgtga ggagaggacg ctcctcgagt cgattggaaa agagatcggc tcaggcatcc
    16981 tgaaaggcat gctccacaag aggcttgagg cggcaaaccg ggaggcgaac ctgtatctcg
    17041 acatcatgac gcacgatatc aagaacgccg agaacgtatc gaacctttac acggatctcc
    17101 tcatcgggat gctggagggc gagaccgcgc agtacgtgag gaggatccag agcagcatcc
    17161 agaagagcgc cggtatcctc aggaacgtca ccacgatccg ccggatccac cacgatgcat
    17221 ccgatctcgg gccgatcgac ctcgacgcgg tcatcagagg ggagatcgag gcctttcccg
    17281 gtatcgacat cgagttttcg ggcattcgct gcccggtctg ggcggacgac ctcctctgcg
    17341 aggtcttctc aaacctcatc cggagtgccg tcaggttcgg gggttcggac atccggatcg
    17401 tcatccgggt ggaggattac gacggcgaga acgtcctcgt ctccatcgag gataccggtc
    17461 cgggtatccc tgactccgt
//
LOCUS       DAOSII010000032        17141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_3532_length_17141_cov_55.341566, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000032 DAOSII010000000
VERSION     DAOSII010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 17141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17141
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3532_length_17141_cov_55.341566"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..2200
                     /gene="fdhF"
                     /locus_tag="PLV90_03960"
     CDS             140..2200
                     /gene="fdhF"
                     /locus_tag="PLV90_03960"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HOF96299.1"
                     /translation="MDIKYVSTTCPYCGTGCGFNLVVRNGKVFDVAHWQRAPVNGGKL
                     CPKGRYAHEFINSPDRLTKPLIKKDGKFVEATWDEAYDLIAERFGSYKPDEMACLSSA
                     RTSNEENYLMQKFARVALKTPHVDHCARLCHSSTVAGLAAVFGSGAMTNSIRDLVESK
                     CIFILGSNTFEQHPLIARSVVRARTNGAKVIVADPRYTPTARQADLYMPFVSGTDVAI
                     LNGLMQEIIRNGWEDKEFITKRTKDYEKLKEVVMKDVYSLENATKVSGIPVESLRTAA
                     EWIAAADVAALIYSMGITQHTTGVDNVKSTANLMMLTGNLGRPGGGVNPLRGQNNVQG
                     ACDMGALPNVYSGYQKVTDEATRTKMREMWGVDDIAEGKAGYTVTEMVNVLADTPGTL
                     KCMYIMGENPMLSDPDIRHVEDALRNLEFLVVQDIFLTETGRLADVILPAACYAEKDG
                     TQTNTERRVQRWRKAQDPPGDAKPDWQILCDLGKRMGYEREFAFENPEAIFAEVTAVT
                     PSYHGMSYARLDPDGLHWPCPTAEHPGTAILHREKFATPDGLGVFSAIEWKPPAEVPD
                     AEYPYVLTTGRVIWQWHTGTMTRRSWSLEKEAPIGWIEINPEDAKTLGIRDNEVVRAS
                     TRRGSIDIPARVTPEIIRGVMFIPFHYKEHPANMLTHNALDPIAKIPEFKACAVRVEK
                     VAEA"
     gene            2204..3469
                     /locus_tag="PLV90_03965"
     CDS             2204..3469
                     /locus_tag="PLV90_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOF96300.1"
                     /translation="MAGKGDLLYAWTTDEALREKAETGGAVSALLRHALTSGMVDGVF
                     AVRKGADVYDAVPALITDPADIEGIAGSLHCGTLLLPKQMRRCLLSAEPDMRIATVLK
                     GCDVKAMYEMAKRNQVNLDNILMIGLNCGGTIRPEVARTIVRERLGLDPDDVVKEEID
                     KGKFIVITKDGQHASISIDELEEGAEDLLGNEGLGRRSNCRRCKIKIPRQADLACGNW
                     GVIGERAGSATFVEVGSEKGANLLNGAVEAGAVATEAAPAKGIEIRGKVENAMLKLGD
                     RWRARYFGALGEGKERLNRIREQTNRCIKCYSCIENCPICYCIDCSTRKEYLVEPGVI
                     PPPFMFHLIRFAHISDSCVNCGQCEELCPVEIANSVFLHAIQTDLEELFGFHPGEDMT
                     PPVLALVEEKPERDRLMATGSDQIFDIFK"
     gene            4257..5129
                     /locus_tag="PLV90_03970"
     CDS             4257..5129
                     /locus_tag="PLV90_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HOF96301.1"
                     /translation="MVFHPPVAIVAKAGDAGKYKVGLPVWNMIVRGFMSGAYIAMGGA
                     LATVCSTGVMATDVALRSGFASAGMAQFMLGAVFPVGLIITIMTGAELFTGDAMLAPM
                     AAFIHKVSWGAVLNLWLWVYVGNLIGSVVFAYIMAYGPCVSFDAAGTATLTAFGVRAI
                     GIATAKTSYVGGMALWSVFVKGIGCNWLVNLAVLLGICADDAIGKIVGIWFPIFAFVA
                     TGFEHSVANMYFIPTGIFCTGLDPTKAVDTVNWVAMWTNNLIPVTLGNIVGGMLFVGI
                     IYWVAFRKEIAALK"
     gene            5163..5651
                     /locus_tag="PLV90_03975"
     CDS             5163..5651
                     /locus_tag="PLV90_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HOF96302.1"
                     /translation="MRVTPVALAVFLFFMILIAVYVFATGDVRSLIWAVPAAVMLILI
                     PVALNYMSQKQYASLVPVYEAEARTTRVREINLNMLGETVRVKGVVERVYFQFLNRPQ
                     YLVADRTGEISVKMFTSPAEDVQKGDVVEVLGMVVKRYLLSGDAVINCVSIRKVEKVQ
                     RS"
     gene            complement(5734..7332)
                     /locus_tag="PLV90_03980"
     CDS             complement(5734..7332)
                     /locus_tag="PLV90_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF96303.1"
                     /translation="MMSLIDIDGDDASKYRLIGDRVLSYRFAVPHDAELYLGDILKIT
                     DSVKGLTFFAKVSDLLHDCNFSDPKWDTRPHTEHFYGIGEDVFILVEAVPLGYIGEDG
                     EFRKPRTIPAKFSRVERPESQDFAFLRQVMGDIEVGVMKTGQGVLKDVRVALHSAVMR
                     QHMGVFATTGMGKSNFMKVFCASCMRVREFGLLIVDPHGEYVAGGRSSSGESTRGLLH
                     YQAGRDGLAVFSTRSEQYRKKYHLDQLFLDYDDFRMPDLLLLYEHSPPQREVVEMLES
                     VPGSDVIDFFQGIDAATFDADTYSGPHRGIARGLKNFSASTLAVMQRRIAGMLNRNRG
                     FFRRQGTSIPDIMRALHENKVALIDIPGMSEQSELFVLSIITRMIMRNHQGEDARSAE
                     EARQVLITIEEAQRVLGSGSGSTRVFREAAMEGRKFGVGLCVVTQQPKNIDARVLAQL
                     NTFVVMGLSDRGDRDMIASSAKQDLSRLDTEIQTLEPGEAVISTIGIPFPVSTRIHLF
                     EDYIGDLSERPGARPIDDGLERTF"
     gene            complement(7310..8347)
                     /locus_tag="PLV90_03985"
     CDS             complement(7310..8347)
                     /locus_tag="PLV90_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HOF96304.1"
                     /translation="MDQNTLYRDAIRRVAGRIREVAPPDLAERFSAAGGFDPASYRHC
                     RPEFGGAVCAVDGSNTIVLDAGCFAVAAVRASVSAYAGGARLYHRTTPLHIVTVNPGT
                     GNDDFDALFYDCFQRPPKVHLDHDDPVRNAAVIRDTLEYRAAMEMAGELDAGDLIVLD
                     GTLQVRHASHDEVVESLLNLCNLRGVLIAAVTKRTSLTWGGGHPIVPAAEGLARGLGI
                     PEPWYLSLSAAGGLLDRRETSAWKQRGEQYVARLHPRADRAFKVEIPRYYDTERVERV
                     FSALASFADDGRVTGYPYPLLDAHLTTRIGRDAVEQVRHDIIRDMDRLGMNLADYTGI
                     FGDYHDEFDRY"
     gene            complement(8347..11520)
                     /locus_tag="PLV90_03990"
     CDS             complement(8347..11520)
                     /locus_tag="PLV90_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HOF96305.1"
                     /translation="MLLNKLAMRNFKRFRNQEIAFQDGITGIVGNNGTGKSSIVAAVL
                     FALYGLQGTGLDANYIVSSFAGPQDVCEVRLDFVAGGNEYAVVRRFKRRPSSTLHEAN
                     LYLNQKLLASSVQKVGEEMQRIIGMGPGDFRNTIYAGQKELLALIESRAGSRKDWFMQ
                     VLGIDYLRKDSMEDLKRIVDAREGVSLELSGRLRELDAASVRDRLAALRADLARAEEE
                     AKRAGAVEKNAIAALDNARGERDRLLFVRDRFLHLAREKEVLATDIARLRTECREAEE
                     EIAGRRRLQADLEALAPLAGRYEPLKAELAAMAETKAARDRLSLEAGAAGEQVREYDE
                     RRARLDAGLATLARDEQTVAGLSREVAEWQALRGRITAMKALQPEYDRLREDLARMDE
                     RFVQTERRVQENRREIETIEEKNRHLRALETEIGDYDGLRAQQEVYLRVLEYSRQEER
                     CLQEVRAGSEEMSHLEGEIAGLARQLAGMAAIEERIESAASRKEYLTSRTSACAERQE
                     TLREEIRRLAGHRADILSAGPEGACPTCRQSLGAHYEELVENLAAATASMQATLADLE
                     YEHARGTIEHQSLAAELADLQQHLTVFRRLKEQHALSSTRYEQSAGIVQRWQAEASEH
                     RAAIRALGMDGYDQAAHAALRERIRYIEGKRTAADTLRGACCRLPALQEERRRLIAEV
                     EGYLARKEDIEAKIARLGFDPEVRQRLEADAEALEASYRACTEAEARLARRPDIEEEL
                     RAVVARADGLRERLRVVTDELARLAFDPERFAHLNEECNRAEAAYRTTLELRVRLEEI
                     PRLAGALKTKQDLLASRETERLQVEKAVNDLDFDERQVAAIEKALAGCERDLFAAREQ
                     EIAIVARITGLSADIEETTGRLARADALLRQQEALTEEIGRLKLTRSLIKDYTDYLLQ
                     VVRDRIEEEAGRVLAEITDGRYSTVMLDDDFTVLVHDMGDDYPADRFSGGEQDDIAIA
                     LRVALSRFLAEVNGVHDSTFLIFDEIFGSQDEGRRNNLLRALRTQEAHFPQILLISHI
                     TEVQDEFSTTLVVEMGADQASRVREFA"
     gene            complement(11507..12667)
                     /locus_tag="PLV90_03995"
     CDS             complement(11507..12667)
                     /locus_tag="PLV90_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HOF96306.1"
                     /translation="MKIVHLADTHLGLSAFNRVDPETGMNLREQLIYDNFLAAVDRVI
                     AMRPDALVHAGDLFHQVKPKTRAYTTALDALSRLQDAGVPVLVVAGNHSMAKTRYTAS
                     PFEVLERGGYAARGLYVAHHNRYRRVELGDTVFHLIPNMLEAEGYRRAFDGLEFSPGT
                     NVLVTHGLASNLKDRRLHTVAEHELDATILSDRFDYIALGHFHGQVQVADNAWYSGSL
                     EYCNYGEIADTKGGLTADLSSGTVTHIDFPHTPMIGLGKINCDGLSAREVMDGILGAV
                     DGAGEKTRRAICQVTLDGIRRETLRALDQKTLAEVRSRMLDLKLQVFAADDPSRIFYG
                     DSLVGLDYVAEFERFVEREHLAPGDEEYVKKTGTTILRSVIARHKEGENAAE"
     gene            12798..12980
                     /locus_tag="PLV90_04000"
     CDS             12798..12980
                     /locus_tag="PLV90_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96307.1"
                     /translation="MSKRAVDAVFQALFILSDIRFLLRETAPQHDLDEAQKKRAATSI
                     EKVKRQIAIIEEELMR"
     gene            12977..>13836
                     /locus_tag="PLV90_04005"
     CDS             12977..>13836
                     /locus_tag="PLV90_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOF96308.1"
                     /translation="MKCAVDIDARDIEELYINIDPIQAGGRLTADAMKAAISFGDGYS
                     VCDNCRNPPRLDYIRNPPIAQFHADLAAWLNMDAVRVVPGARRGFQAVASTLVSKGDP
                     VIVTALAHYTEFMAVEAAGGIPREVPKNELNHITPDAAAEKIEAVIREFSRTPPLLFV
                     DHVDYQFGNVHDVAGIIKVAHQYDIPVLVNGAYTVGIMPVDGKALGADFVVGSGHKSM
                     AAPAPSGLLATTSEHADRVFRMTQAKGDVTGRTFGMKEVEMMGCTLMGVTVVGMMASF
                     PHVKERVKRW"
     assembly_gap    13837..13936
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14059..15228)
                     /gene="thiI"
                     /locus_tag="PLV90_04010"
     CDS             complement(14059..15228)
                     /gene="thiI"
                     /locus_tag="PLV90_04010"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HOF96309.1"
                     /translation="MEAVMIRYGEIFLKSETVKRRFISIMTENIRLALEAKGLSHRIE
                     TPRGRILIFGDEPRRIAAAVAGTFGVVSASVCTMTSSDIDTIAAVAVEHAGRHLRPGM
                     SFAVRARRSGVEGFNSQELAAAVGSAILDRVPEATVDLSTPDYEVFVEAREFGGLVYD
                     ERLKGPGGLPYGTQGEVVALLSEGIDSPVASWLMMRRGCRVVHVHMHGGRFGGTDGEE
                     TVLRHHARLSAWVPGHPLDLLVVDMEPFFEVIIALKEPRYRCILCKRFMLRVAGILAE
                     ERGAYALVNGDNLGQVASQTLANMAVIAPAASVTVLRPLIGFDKEEIVERARAIGTFE
                     ARAGSTGCTVAPRYPSTAAPAATIVDIEEKIGAKDLAARAAGTVRVYRAKNGEIA"
     gene            complement(15216..15992)
                     /gene="larE"
                     /locus_tag="PLV90_04015"
     CDS             complement(15216..15992)
                     /gene="larE"
                     /locus_tag="PLV90_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOF96310.1"
                     /translation="MTHDADLALLLKSCEPIAIALSGGTDSSVLLAYARRHGIRTIAI
                     SVDTGLTPPGELEAARVLSERLGIPHIVIPLDALDIPAVRENRPDRCYACKRAMMEAV
                     VREAERRGCRAVVDGTHADDRSAARPGMRALAELGIRSPFAECDIGKKDIEALAADLG
                     VPVRPPSACLATRIPAGDAVTRECLALVAAAEALIAKEIPGTIRVRCIGEGARASIEA
                     DPAYHDRLEELLDAVRDLGFEDAVIASGGYREGGADAWKR"
     gene            16107..16733
                     /locus_tag="PLV90_04020"
     CDS             16107..16733
                     /locus_tag="PLV90_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2150 family protein"
                     /protein_id="HOF96311.1"
                     /translation="MAKKAKAKQAEPMKLFYIFYNQERWDNWIKTLEGADFEPAEGED
                     VSEGEQMLYSFTEDITLSVLKIIRLYQNDRFTKEEAIAKLDDVELIVMTGLPEGELED
                     IIGSLQLSLLVLFTACRKYIEGEFDKDIKALVKKGRGIDEENFEEALEVAANIGAAVV
                     DGATCCAKYIKDDAENPGLFDEWLIEIETMSNAMKSLAKFDEEPGEAS"
     gene            16730..>17141
                     /locus_tag="PLV90_04025"
     CDS             16730..>17141
                     /locus_tag="PLV90_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5814 domain-containing protein"
                     /protein_id="HOF96312.1"
                     /translation="MIADKARFRAARKLERAAGFRLPDHVFSGAFLESLGKAINFEHL
                     DRRMQEQLLAFFRDFMDCKCKNAPFCGCPERKFTLAIIEFREMGLDHRQIGAHLLDEY
                     GIDLYPADILSFLEDSVHMLEAIRDVAELQGREKL"
BASE COUNT         3145 a         5372 c         5325 g         3199 t
ORIGIN      
        1 cctgccgttt cggggtgacc ccgggcagga catataccgt gtatttgccg tatatcgtta
       61 atttacctcg aaaaccacgt tggaccatgc ctattatatg acacgggcgg tatgttctcc
      121 ctgccagagg ggaacgacaa tggatatcaa gtatgtatcg acaacgtgcc cgtactgcgg
      181 caccgggtgc gggttcaacc tcgtcgtccg gaacggcaag gtctttgacg tggcgcactg
      241 gcagcgcgca cccgtcaacg ggggtaaact ctgccccaaa ggacgttacg cccacgaatt
      301 catcaacagc cccgaccgcc tgacaaaacc gctcatcaag aaggacggca agtttgtcga
      361 ggcgacctgg gacgaggcct acgacctgat cgcggaacga ttcggatcct acaaacccga
      421 cgagatggcc tgcctctcct ccgcccggac atcgaacgaa gagaactacc tgatgcagaa
      481 gttcgcccgg gtggcgctca agacgccgca cgtcgaccac tgcgcccggc tctgccactc
      541 gtccaccgtc gccggcctcg cggcggtctt cgggtccggg gcgatgacga actcgatccg
      601 ggatctcgtc gagtcgaagt gcatcttcat actcgggtcc aatacattcg agcagcaccc
      661 cctgatcgcg cggagcgtcg tccgcgcgag aacgaacggt gcgaaggtga tcgtggccga
      721 cccgcgctac acgccgaccg cccggcaggc cgacctctac atgccgttcg tctccgggac
      781 cgacgtcgcc atcttgaacg ggctgatgca ggagatcatc aggaacggtt gggaggacaa
      841 ggagttcatc acgaagcgga cgaaggatta tgagaaactc aaggaggtcg tcatgaagga
      901 tgtctacagc cttgagaacg ccacgaaggt ctccggtatt cccgtcgaga gcctcaggac
      961 cgcggcagag tggatcgcag ccgccgacgt cgccgcgctc atctactcga tgggcatcac
     1021 ccagcacacc accggcgtcg acaacgtcaa atcgaccgcg aacctcatga tgctcaccgg
     1081 caacctgggc aggcccggcg gcggcgtgaa cccgcttcgc ggccagaaca acgtccaggg
     1141 cgcctgcgac atgggagccc tcccgaacgt ctactccggc taccagaagg tcaccgacga
     1201 ggcgaccaga acgaagatga gggagatgtg gggcgtcgac gatatcgccg agggtaaggc
     1261 cggttacacc gtcaccgaga tggtgaacgt ccttgccgat acgcccggca cgctcaagtg
     1321 catgtacatc atgggcgaga acccgatgct ctccgacccg gacatccggc acgtcgagga
     1381 tgcgctcagg aacctcgagt tcctggtcgt tcaggacata ttcctgaccg agaccggccg
     1441 tcttgccgat gtgatcctgc ccgccgcctg ctacgcggag aaagacggca cccagacgaa
     1501 caccgagcgg cgggtccagc gctggaggaa ggcccaggat ccaccggggg atgcgaaacc
     1561 cgactggcag atcctctgcg acctcgggaa gaggatgggt tacgagagag aattcgcgtt
     1621 cgagaaccct gaggcgatct ttgccgaagt caccgccgtc accccgtctt accacggcat
     1681 gtcctacgcc cgcctcgacc cggacggcct gcactggccc tgtccgaccg cggagcaccc
     1741 gggaacggcc atcctccacc gggagaagtt cgccacgccg gacggcctcg gggtcttctc
     1801 cgctatcgaa tggaaacctc ccgccgaggt ccccgacgcc gagtatccgt acgtcctcac
     1861 gaccgggcgg gtcatctggc agtggcacac cggcacgatg actcgccggt cgtggagcct
     1921 tgagaaagag gcgccgatcg gctggatcga gatcaatccc gaggatgcaa agacgctcgg
     1981 catcagggac aacgaggtcg tccgggcaag cacccgccgc gggtccatcg atatccccgc
     2041 cagggtcacc ccggagatca tcaggggcgt catgttcatc ccgttccact acaaggagca
     2101 cccggcgaat atgttgaccc acaacgcgct cgacccgatc gcgaagatcc ccgagttcaa
     2161 ggcgtgtgcg gtcagggtcg aaaaggttgc ggaggcgtga gatatggcag gaaaaggcga
     2221 tttgctctat gcatggacga cggatgaggc gctcagggag aaggcggaga ccggcggagc
     2281 ggtcagcgca ctgttgcgcc acgctcttac gagcggtatg gtcgacgggg tctttgcggt
     2341 caggaagggc gcggatgtct atgatgcggt gccggcgctg atcaccgacc ccgccgatat
     2401 cgaggggatt gcaggctcgc tccactgcgg caccctcctc ctcccgaaac agatgcgccg
     2461 ttgtcttctc tccgccgagc cggatatgcg tatcgcgacg gtcctcaagg gttgcgacgt
     2521 caaagcgatg tacgagatgg cgaagcgcaa ccaggtcaat ctggataaca tcctgatgat
     2581 tggcctcaac tgcggcggca ccatccggcc ggaagtcgcc cggacgatcg tccgggagag
     2641 actcggcctt gaccccgacg atgtcgtcaa ggaggagatc gacaagggta agttcatcgt
     2701 catcacgaag gacggccagc acgcatccat ctcgatcgac gaactcgagg agggggcaga
     2761 agacctcctc gggaacgagg ggctcggccg ccggtccaac tgccgccggt gcaagatcaa
     2821 gatcccgcgc caggcggacc tcgcctgcgg caactggggg gtcatcggtg agcgcgccgg
     2881 cagtgccacg tttgtcgagg tcggctccga gaagggcgca aacctcctca acggcgctgt
     2941 cgaggccggc gcggtggcga cggaggcggc gccggcaaag ggtatcgaga tccgtggcaa
     3001 ggtcgagaac gcgatgctga agctcggcga ccggtggcgg gcgcgctact tcggggcgct
     3061 cggcgagggt aaggagcgcc tgaacaggat ccgggagcag acgaaccggt gcatcaagtg
     3121 ttactcctgc atcgagaact gcccgatctg ctactgcatc gattgcagca cacggaagga
     3181 gtacctggtc gagccgggcg tgatcccgcc gccgttcatg ttccacctga tccggttcgc
     3241 gcacatatcc gactcctgtg tcaactgcgg ccagtgcgag gagctctgcc ccgtcgagat
     3301 agcgaactcg gtcttcctcc acgccatcca gaccgatctc gaggaactct tcgggttcca
     3361 cccgggcgag gatatgaccc cgccggtgct cgcgctcgtc gaggagaaac ctgagcgcga
     3421 ccggctcatg gccaccggca gcgaccagat attcgacatc ttcaagtaac ccctgcccgc
     3481 aggataggag tccgggggat ggtcagatga gttggaaccc gggaaagtga atctcccggg
     3541 tttccccgtg cgacccaccc ccgggttccc ccaagatgcc ttacagcgca cctatagctc
     3601 atgatcttcc cgggctggtg tttcggattg tcagcatggc gttgtggtgc cagaagaaag
     3661 gtgtgtgcgt ctcagcggga tgtagcgccg ggacgagggc tcgtggcgaa ggatcccctg
     3721 gcaccgggtg acggacctgg tggggggccg caggcaagac gctccagttt ccctgaatct
     3781 cccggatcag gcctcgctcc agggctccaa gcccgctcga aaaacgagag gtacggcctg
     3841 aatataccaa aaaaagagta attcccgggt atcacacccg ggtcgtggtg gcatccggcg
     3901 acgcgcaggc atccgcgacg attttagtgg gcgatcatcg ccccacgaga ggaagagcag
     3961 gaggttggag gagctgcgcg cctgctcccg cacgctcgct caaacatcac cctaaaacta
     4021 cctatattcc gcgtggtctc aaaaacaaat ccagaagcgt cgtttatcga ttcttgatgt
     4081 ttaattacgg gaattaactc aacccgatga taaacgccta aatttgggtt atactcttcc
     4141 tgcgtgacga tcaattcgaa acttattaat atccctctca aattactgat taattagacc
     4201 tatacagtct gattacttga tggtataggt aggacgttta gagaggtgta tgaaaaatgg
     4261 tgttccatcc tccagttgct atcgtagcaa aagcgggtga tgccggaaag tacaaggtcg
     4321 ggctgcccgt ctggaacatg atcgtgcgtg gcttcatgtc tggagcctac atcgccatgg
     4381 gcggtgcgct cgcaacagtc tgttcgaccg gcgtcatggc aaccgacgtc gcactgagat
     4441 ccggtttcgc aagcgccggt atggcccagt tcatgctggg tgccgtcttc cctgtggggc
     4501 ttatcatcac gatcatgacc ggtgcagaac tcttcaccgg cgacgcgatg ctcgccccca
     4561 tggcagcgtt catccacaag gtcagctggg gcgctgtgct caatctctgg ctctgggtgt
     4621 atgtcggcaa cctcatcggg tcggtagtct ttgcgtatat catggcatac ggcccgtgtg
     4681 tctccttcga tgccgccgga accgcaacac tcaccgcatt cggtgtgaga gcgatcggca
     4741 tagcaacggc aaagacgagt tacgtcggcg gtatggctct atggtcggta ttcgtcaagg
     4801 gtattggttg taactggctc gtcaaccttg ctgtcctgct cggtatatgc gctgacgatg
     4861 cgataggcaa gatcgtcggt atctggttcc cgatcttcgc tttcgtcgcc accgggtttg
     4921 aacacagtgt tgcaaacatg tatttcattc ccacgggcat cttctgtaca ggtcttgacc
     4981 ccacaaaggc agtcgacaca gtcaactggg tagccatgtg gaccaacaac ctgattccag
     5041 tcacgcttgg caacatcgtc ggcggtatgc tctttgttgg tatcatctac tgggtcgcgt
     5101 tcagaaagga aattgcagcc ctcaagtaaa cctgattgac aatgcagatt ggagatatta
     5161 gagtgagggt gacgccagtt gcactggccg tcttcctctt ttttatgatc ctgattgcgg
     5221 tgtacgtctt tgctaccgga gatgtacgtt cgctgatctg ggcggttccg gcagcagtca
     5281 tgctgatcct cataccggtg gcgctcaact acatgagcca gaaacagtat gcatcgctgg
     5341 tgccggtcta tgaagcagaa gcacgaacaa cccgcgtccg cgagatcaac ttaaacatgc
     5401 tcggcgagac ggtgcgcgtc aaaggcgtcg tcgagcgggt atacttccag ttcctcaacc
     5461 ggccgcagta tctcgtcgcc gaccggacgg gtgagatatc ggtcaagatg ttcacctccc
     5521 cggcggaaga tgttcagaaa ggggatgtgg tcgaggtact gggaatggtc gtcaaacgct
     5581 acctcctgtc cggggatgcg gtcatcaact gcgtctccat ccggaaagtc gagaaagttc
     5641 agcgatcctg acgcctcagg gcgccagagt gttcagtgaa gggagggttg acacgggata
     5701 gaaccctgcc gccatcacct tcttgcattc ctgctaaaat gttctctcaa ggccgtcgtc
     5761 tatcggtctt gcccccggcc tttcgctgag gtctccgatg tagtcctcga agagatggat
     5821 ccgggtgctc acgggaaacg gtataccgat ggtgctgatg accgcttctc ccggctcaag
     5881 cgtctggatc tcggtatcca ggcgagagag gtcctgtttt gcgctgctcg ctatcatgtc
     5941 gcggtccccc cggtcggaaa gccccatcac gacgaacgta ttcagttgcg caaggacccg
     6001 ggcgtcgatg ttctttggct gctgggtaac cacacagaga ccgacaccga acttcctccc
     6061 ctccatcgcg gcctcccgga agacccgggt gctcccggac cccgacccga gcaccctttg
     6121 cgcctcctcg atggtgatca gcacctgcct cgcctcttct gcgctccttg catcttcgcc
     6181 ctggtggttg cgcatgatca tccgggttat gatcgagagg acgaagagtt cgctctgctc
     6241 gctcataccg ggtatatcga tcagggcgac tttgttctcg tgaagcgccc tcatgatatc
     6301 cggtatcgag gtgccctgtc tccggaagaa accccggttc cggttgagca tgcccgcgat
     6361 gcgccgctgc atgacggcaa gcgtgcttgc ggagaagttt ttcaggcccc gggcaatccc
     6421 gcggtgcggg ccgctgtagg tatcggcatc gaaggtcgcg gcatcgatcc cctggaagaa
     6481 gtcgatcacg tctgagcccg gcacgctctc gagcatctcc acgacctcgc gctggggtgg
     6541 agaatgctcg tagaggagga ggaggtcggg cattcgaaag tcgtcgtagt cgagaaagag
     6601 ctggtccagg tgatacttct tccggtactg ctccgaccgg gtggagaaga cggcaaggcc
     6661 gtcacggccc gcctgatagt gcaggagccc tctcgtcgac tctccgctcg acgatcgccc
     6721 tccggccacg tactcgccgt gcgggtcgac gatgaggagc ccgaactccc gcacccgcat
     6781 gcaggaggcg cagaagacct tcatgaagtt gctctttccc atccccgtcg tcgcaaagac
     6841 acccatgtgc tgccgcatca ccgccgagtg gagcgccacc cttacatcct tcaggacacc
     6901 ctggccggtc ttcatcacgc cgacctcgat atcgcccatc acctgccgga ggaacgcgaa
     6961 gtcctgcgac tccggccgct ccacgcggga gaactttgcc gggatcgtcc gcggttttcg
     7021 gaattcgcca tcctccccga tgtaaccgag agggaccgcc tcgacgagga tgaagacgtc
     7081 ctcccctata ccgtaaaagt gctcggtatg cgggcgggtg tcccacttcg ggtcggagaa
     7141 gttgcagtcg tggaggagat cgctgacttt ggcaaagaac gtcagccctt tgacgctgtc
     7201 ggtgatcttc aagatatccc caaggtagag ctccgcatcg tggggaacgg caaaccggta
     7261 gctgagcacc cggtctccga tcaggcggta cttcgaggcg tcgtcgccgt caatatcgat
     7321 caaactcatc atggtaatca ccgaagatgc cggtgtaatc ggcgaggttc atgccgagcc
     7381 ggtccatatc gcggatgatg tcgtggcgga cctgctcgac cgcatccctc cctatccgtg
     7441 tggtgaggtg ggcgtcaagg agcggatacg ggtaacccgt gacccttccg tcgtcggcaa
     7501 atgaggcgag cgcggagaag acccgttcta cccgctccgt gtcgtaatac cgggggatct
     7561 ccactttgaa cgcccgatcg gcccgagggt gcagccgcgc gacgtactgt tcgccccgct
     7621 gcttccaggc ggaggtctcc cggcggtcga gcaggccccc ggcggcggag agagagagat
     7681 accagggctc ggggatgccg aggccgcggg caagcccctc cgccgccggg acgatcgggt
     7741 gccccccgcc ccaggtgagc gacgtccgtt tcgtcacggc ggcgatgagc accccccgga
     7801 ggttgcagag gttcaggagg ctctcgacga cctcgtcgtg gcttgcgtgg cggacctgga
     7861 gcgtgccgtc gaggacgatg aggtcgccgg catcgagttc tccggccatc tccatcgccg
     7921 cccggtactc gagggtatcc cgtatgacgg cggcgttcct cacggggtcg tcgtgatcga
     7981 gatgcacttt cgggggacgc tggaagcagt cgtaaaagag cgcatcgaag tcgtcgttcc
     8041 cggtcccggg gttgaccgtg acgatatgga gaggcgtcgt cctgtgatag aggcgcgcac
     8101 caccggcata cgcgctgacc gatgcccgga cggcggcgac ggcaaaacat cccgcgtcga
     8161 ggacgatggt gttgctcccg tcgacggcgc agacggcgcc gccgaactcg ggccgacagt
     8221 gccggtagga ggcggggtca aacccgccgg ctgccgagaa ccgctccgca agatccggcg
     8281 gggcgacctc acggatcctt cctgcgaccc gccggatggc gtcccggtag agggtgttct
     8341 ggtccatcac gcaaactccc ggacccggct tgcctgatct gcccccatct cgaccacgag
     8401 cgtcgtcgag aactcgtcct ggacctcggt gatgtgagat atgaggagga tctgggggaa
     8461 atgggcctcc tgggtcctga gagcgcggag aaggttgttc cgccgtccct cgtcctggct
     8521 cccgaatatc tcgtcgaata taaggaacgt ggagtcgtgc accccgttca cctcggcaag
     8581 gaaccgggag agggcgacac ggagggcgat cgctatatcg tcctgctctc ccccgctgaa
     8641 ccggtcggcc gggtagtcgt cccccatatc gtggacgagg acggtgaagt cgtcgtcgag
     8701 catcacggtg ctgtacctcc cgtcggtgat ctccgcaagc accctcccgg cctcctcctc
     8761 gatccggtcc ctgaccacct ggaggaggta gtcggtgtaa tctttgatga gggatcgggt
     8821 gagtttcagg cgcccgatct cctcggtgag cgcctcctgc tgccggagga gggcatccgc
     8881 acgggcaagc ctcccggttg tctcctcgat atcggccgag aggccggtta tgcgggcaac
     8941 gatcgcgatc tcctgttccc gtgcggcgaa gagatccctt tcgcatcctg cgagcgcctt
     9001 ctctatcgcc gcgacctgcc gctcgtcgaa gtcaaggtcg tttacggcct tctcgacctg
     9061 gagccgttcg gtctcccggc tcgcgaggag gtcctgcttt gtcttgagcg ccccggcgag
     9121 ccgcgggatc tcttcgagcc ggacgcggag ctccagggtc gtcctatacg ccgcctcggc
     9181 acggttgcac tcttcgttga ggtgcgcgaa cctctccggg tcgaacgcga ggcgggcgag
     9241 ttcgtccgtg acgacccgca acctctcgcg gagcccgtcg gccctggcaa cgacggctct
     9301 caactcctcc tctatgtccg gtctccgcgc aaggcgggcc tctgcttccg tgcatgcccg
     9361 ataggatgct tcaagcgcct ccgcatccgc ctcgagacgc tgccggacct ccgggtcgaa
     9421 cccgagccgg gcgatcttcg cctcgatatc ctccttgcgg gcgaggtaac cctcgacctc
     9481 ggcgatgagc cgtcgccgct cctcctggag ggcggggagg cggcagcacg cgccccggag
     9541 ggtatcggcc gccgtccgct tcccctcgat ataccggatc ctctccctga gggcggcgtg
     9601 cgcagcctgg tcgtacccgt ccatgccgag cgcccggatt gcggcacggt gctcgcttgc
     9661 ctcggcctgc cagcgctgca cgatacctgc gctctgctcg taccgggtcg aggagagggc
     9721 gtgctgctct ttcaagcgcc ggaagacggt gaggtgctgc tggaggtcgg caagctcggc
     9781 ggcgagggac tggtgttcga tggtcccccg cgcatgctcg tactcgaggt ctgcaagcgt
     9841 cgcctgcatc gatgcagtgg cggcggcgag gttctcgacc agctcctcgt agtgagcgcc
     9901 gaggctctgg cggcacgtcg ggcaggctcc ttccgggccc gcagagagga tatccgcccg
     9961 gtgcccggcg agccgccgga tctcctcgcg aagcgtttcc tggcgttcgg cgcatgcgct
    10021 ggttcgtgaa gtcaggtact ccttccggct tgccgccgac tcgatccgct cctcgatggc
    10081 ggccatcccg gcgagctgcc gggcaagtcc ggcgatctcg ccctcaagat ggctcatctc
    10141 ctcggacccg gcgcggacct cctgcaggca ccgctcttcc tgccgggagt actcaagaac
    10201 cctgagatac acctcctgct gggcccggag accatcgtag tcgccgatct cggtctcgag
    10261 cgcacgaaga tgccggttct tctcctcgat cgtctcgatc tccctccggt tctcctggac
    10321 acggcgctct gtctggacga accgctcgtc catgcgggca aggtcttcgc ggagacgatc
    10381 gtattccggc tgaagggcct tcatcgccgt gatacgaccg cggagggcct gccactccgc
    10441 gacctcccgg gaaagcccgg caactgtctg ttcgtcccgg gcaagcgtgg cgagcccggc
    10501 atcgaggcgc gcgcgacgtt cgtcgtactc ccgcacctgc tcgcccgcgg cgccggcctc
    10561 gaggctcagg cggtcgcgtg cagccttcgt ctccgccatc gcggcaagct ccgccttcag
    10621 gggttcgtag cgcccggcaa gcggggcaag ggcttcgagg tcggcctgca gtctcctgcg
    10681 tcctgcgatc tcctcctcgg cctcccggca ctcggtgcgg agccgcgcga tatcggttgc
    10741 gagcacctct ttttcccgcg caaggtgcag gaagcgatcg cgcacaaaga gcagccggtc
    10801 acgctccccc cgggcgttat cgagcgccgc gatggcgttc ttctctacgg cgccggcacg
    10861 ctttgcctcc tcctccgcac gggcaaggtc ggcgcggagc gccgcaaggc ggtcgcgcac
    10921 ggaggcggcg tcgagctccc ggaggcgccc ggagagctcc agagagaccc cttcccgggc
    10981 atcgacgatc cgcttcaggt cctccatgct gtccttcctg aggtaatcga tgccgagcac
    11041 ctgcatgaac cagtctttcc gggacccggc ccgactctcg atcagggcga ggagctcctt
    11101 ctgcccggca tagatggtgt ttcggaagtc acccggaccc atgccgatga tccgctgcat
    11161 ctcctccccc accttctgga cgctgcttgc gaggagcttc tggttcaggt agaggtttgc
    11221 ctcgtggagg gtcgaggagg ggcggcgctt gaacctgcgg acgacggcgt attcgttgcc
    11281 gccggccacg aagtccaggc ggacctcgca gacgtcctgc gggcccgcaa aggagctgac
    11341 gatgtagttc gcgtcaagcc ccgtcccctg gaggccgtag agggcaaaga ggacggcggc
    11401 gacgatgctg ctcttgccgg tcccgttgtt cccgacgatc ccggtgatgc cgtcctggaa
    11461 ggcgatctcc tggttgcgga accgcttgaa gttgcgcatg gcaagtttat tcagcagcat
    11521 tctctccctc cttatgccgc gcgatgaccg agcggaggat cgtcgtcccg gtcttcttca
    11581 cgtactcctc gtcgcccggg gcgaggtgct ctctctcgac gaaacgctcg aactcggcga
    11641 cgtagtcgag gccgacgagc gagtctccat aaaagatacg ggagggatcg tcggcggcga
    11701 acacctgcag tttgaggtcg agcatccggc tccggacctc cgcgagggtt ttctggtcga
    11761 gcgcgcggag cgtctcgcgg cgtatcccgt caagcgtgac ctggcagatc gcccggcggg
    11821 tcttctcccc tgcgccgtca accgcgccga ggatcccatc catgacctcg cgggcggaga
    11881 ggccgtcgca gttgatctta ccgaggccga tcatgggggt gtgcgggaag tcgatatgcg
    11941 tgaccgttcc ggacgagagg tccgcggtaa ggcctccttt ggtatccgcg atctcgccgt
    12001 agttacagta ttcgagcgac ccgctgtacc aggcgttgtc ggcgacctgc acctggccgt
    12061 ggaagtggcc gagcgctatg taatcgaacc ggtcggagag gatcgtcgca tccagttcgt
    12121 gctcggcgac ggtgtgcaac cgccggtctt tcaagttgct cgcaaggccg tgggtgacga
    12181 ggacgttcgt gccgggcgag aactcgagtc catcaaacgc cctccggtaa ccttccgcct
    12241 cgagcatgtt cggtatgagg tgaaagaccg tatctcctag ttccacccgc cggtaacggt
    12301 tgtggtgggc gacgtagaga ccgcgtgcag cgtagccgcc ccgctcgagc acctcgaacg
    12361 gggacgccgt gtaacgggtc tttgccatgc tgtggttgcc ggcgaccacg agcaccggaa
    12421 ctcccgcatc ctgcaggcgg gagagggcgt cgagcgccgt cgtgtaggcg cgggtcttcg
    12481 gtttgacctg gtggaagagg tcgcccgcat ggacgagcgc atcggggcgc atggcgatga
    12541 cccgatcgac ggcggcgagg aagttgtcgt agatgagctg ttcgcgcagg ttcatcccgg
    12601 tttccggatc gacccggttg aacgccgaca acccgagatg cgtgtctgct aaatgaacga
    12661 tcttcatgcc caattaatct ccgtgtgttg cgggtataaa tggctgctgc gtgcggtgtg
    12721 aaagtgttcg atgccgggcc gatgcccgga atacgcgtaa cctaatatat gctcaatgat
    12781 atttgtatgg tgatctcgtg tcaaaaagag ccgttgatgc agtttttcag gcactcttca
    12841 tcctctccga catccggttc ctgctccggg agaccgctcc gcagcatgac ctcgatgaag
    12901 cgcagaagaa gcgtgccgca acctccatcg agaaggtgaa gcggcagatt gccatcatcg
    12961 aagaggagct gatgcggtga agtgcgcagt cgatatcgat gcccgcgaca tcgaggagct
    13021 gtacatcaac atcgatccca tccaggcggg cgggcgactc accgccgatg ccatgaaggc
    13081 ggcaatatcg ttcggcgacg gctactcggt ctgcgataac tgtcggaacc ccccccggct
    13141 cgactacatt cgaaaccctc ccatcgcgca gttccacgcg gacctcgcgg catggctgaa
    13201 catggatgcg gtgcgggtgg tgccgggagc gcgccggggg ttccaggcgg tcgcctccac
    13261 gcttgtctcg aagggcgacc cggtcatcgt gacggcgctt gcccactata cggagttcat
    13321 ggcggtcgag gcggcgggcg ggatcccgcg cgaggtcccg aagaatgagt tgaatcatat
    13381 cacccctgac gccgctgcag agaagatcga ggcggtgatc cgtgagttct cgcgcacacc
    13441 cccgctgctc ttcgtcgacc acgtcgacta ccagttcggg aacgttcacg acgtcgcggg
    13501 gatcataaag gtcgcccacc agtacgacat cccggtcctc gtcaacgggg cctacacggt
    13561 cgggatcatg cccgtcgacg gcaaagcgct cggcgccgac ttcgtcgtcg ggtcgggcca
    13621 caagagcatg gccgccccgg cgccctcggg gctcctcgcg acgacgagcg agcacgcgga
    13681 tcgagtcttt cggatgacac aggcgaaagg ggacgtgacc ggcaggacgt tcggcatgaa
    13741 ggaggtggag atgatgggct gcaccctgat gggcgtcacc gtcgtcggta tgatggcctc
    13801 cttcccccat gtcaaagagc gggtgaagcg ctgggannnn nnnnnnnnnn nnnnnnnnnn
    13861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13921 nnnnnnnnnn nnnnnnacct tcgggctgac cgataagcag acccggcacg tcggggagtc
    13981 gtttgtcgcg gttgcccggg agaacgggct gaatattgtc tgaagatccc gaaccttttt
    14041 ttcgggtgga aaaaattctt acgcaatctc cccgttcttc gcgcgatata cccgcaccgt
    14101 ccccgccgcc cgggccgcga ggtctttcgc gccgatcttc tcctcgatat cgacgatcgt
    14161 cgctgcaggt gccgccgtcg aggggtaacg gggggcgacc gtgcacccgg tgctccccgc
    14221 ccgggcctca aacgtcccga tggcgcgggc gcgttcgacg atctcctcct tatcaaaccc
    14281 gatcagcggc cggagcaccg tcaccgatgc cgccggggcg ataaccgcca tgttcgcgag
    14341 cgtctgggac gccacctgcc ccaggttgtc gccgttgacg agcgcgtagg cgccgcgttc
    14401 ttctgcaagg atgcctgcca ccctgagcat gaaccgcttg cagaggatac accggtaacg
    14461 gggctctttt aaggcgatga tgacctcaaa gaacggctcc atgtccacga cgaggaggtc
    14521 gagaggatgc cccggaaccc aggccgagag ccgggcgtgg tgccggagaa ccgtctcttc
    14581 cccgtccgtt ccgccgaacc gtccgccgtg catgtggacg tgcaccaccc ggcacccccg
    14641 gcgcatcatc agccacgatg caacgggcga gtcgatcccc tccgagagca gggccaccac
    14701 ctccccctgc gtcccgtaag ggagccctcc cggccccttg agcctctcgt cgtagacgag
    14761 gccgccgaac tcccgggcct ccacgaagac ctcgtaatcg ggcgtcgaga ggtccaccgt
    14821 ggcctccggc acccggtcga ggatggccga gccgaccgcc gccgcaagtt cctggctgtt
    14881 gaacccctcg acgcccgacc ggcgtgccct gaccgcaaac gacatccccg gccggaggtg
    14941 ccgtcctgcg tgctctacgg ctacggccgc gatagtatcg atatcggaag aggtcatcgt
    15001 gcagacgctc gcgctcacca ccccgaaggt tccggcgacg gccgcggcga tgcgccgggg
    15061 ttcgtcgccg aagatcagga tccgcccccg gggcgtttcg atgcggtggg agagcccttt
    15121 tgcctccagc gcgagccgga tgttctccgt catgatggat atgaaccgcc gtttcaccgt
    15181 ctcgctcttg aggaatatct cgccgtacct gatcatcacc gcttccatgc gtccgcacct
    15241 ccctcgcggt agcctccgga ggcgatgacc gcatcctcaa acccgaggtc ccttaccgca
    15301 tcgagcagtt cttccagcct atcatggtat gcgggatcgg cctcgatgga ggcccgggcg
    15361 ccctccccga tgcaccggac ccgtatggtc ccggggatct ctttcgcgat gagagcttcc
    15421 gcagccgcga cgagcgcgag gcactcacgg gtgaccgcat cgccggcggg gatgcgcgtc
    15481 gcaagacatg cggacggtgg gcggacgggc accccgaggt ctgccgcaag ggcctcgata
    15541 tccttctttc ctatgtcgca ctccgcaaac gggctcctta tcccgagttc ggcgagtgcc
    15601 cgcataccgg gccttgcggc ggatcggtcg tcggcgtggg tcccgtcgac caccgcccgg
    15661 catccgcgcc gctctgcctc gcggacgacc gcctccatca tcgccctctt gcaggcgtag
    15721 caccggtccg gccggttctc ccggaccgca gggatatcga gcgcatcgag cggtataacg
    15781 atgtgcggga tgccgagacg ctccgagaga acccgcgccg cctcgagttc ccccgggggg
    15841 gtaaggccgg tatcgacgct gatagcgatc gtccggatgc cgtgccgccg ggcataggcc
    15901 agcaggaccg aactgtctgt tcccccggag agggcgatcg caatgggttc gcaggatttc
    15961 aggagcagcg cgagatccgc atcgtgtgtc atctgctcag cttatgctcg cctggcctac
    16021 atgaaggttc gcgaatcgcg gcaattcggg ctttcggcac agagagcaaa tgatttttaa
    16081 aagaggcact ccgaatgtgt actgatatgg cgaagaaagc gaaagccaaa caggcagaac
    16141 ccatgaaact cttctatatt ttttacaatc aggagcgctg ggacaactgg atcaagacac
    16201 tggagggtgc tgacttcgag cccgccgaag gcgaagacgt ctccgaaggc gagcagatgc
    16261 tctacagttt caccgaagat atcaccctct cggtcttgaa gatcatccgc ctctaccaga
    16321 acgaccggtt cacgaaggag gaggccatcg cgaagctcga cgacgtggaa ctgatcgtca
    16381 tgacggggct tcccgaggga gaactcgagg atatcatcgg gtcgctccag ctctccctgc
    16441 tcgtactctt caccgcctgc cggaagtata tcgagggcga attcgataaa gacatcaagg
    16501 cgctcgtgaa gaaaggccgg ggcatcgatg aggagaactt cgaggaagct cttgaggttg
    16561 cggcaaatat cggcgccgcc gttgtcgatg gcgccacctg ttgcgcgaag tacatcaagg
    16621 acgatgcgga gaaccccggc ctcttcgacg aatggctcat cgagatcgag accatgagca
    16681 acgccatgaa gtctctcgcg aagttcgacg aggagcccgg cgaggcgtcg tgatcgcgga
    16741 taaggcccgg ttccgggcgg cgcggaagct ggagcgggcg gcaggattcc ggctgccgga
    16801 ccatgtcttc tcgggcgcat tcctcgaatc gctcgggaag gcgatcaact tcgagcacct
    16861 cgaccgccgg atgcaggagc agctccttgc ttttttccgc gatttcatgg actgcaagtg
    16921 taaaaacgcc cccttctgcg ggtgcccgga gcggaagttc accctcgcca tcatcgagtt
    16981 ccgggagatg gggctcgatc atcgccagat cggcgcccat ctcctcgacg aatacgggat
    17041 cgacctctac cccgccgata tcctgagttt cctcgaagac tccgttcaca tgctcgaggc
    17101 gatacgggac gtcgccgaac tgcaggggag ggagaaactc g
//
LOCUS       DAOSII010000033        16430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_3702_length_16430_cov_44.913954, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000033 DAOSII010000000
VERSION     DAOSII010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 16430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16430
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3702_length_16430_cov_44.913954"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1500)
                     /locus_tag="PLV90_04030"
     CDS             complement(130..1500)
                     /locus_tag="PLV90_04030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOF96313.1"
                     /translation="MEREYDVVVIGTGVAGSDVAWHCGDAGMRVAITDRREYGGTCAL
                     RGCVPKKVLAGAAEVVARVHDQQGNGVRGTVSIDWPALIAFVRTFTGPTPRRKEESLR
                     EAGVDTYHESARFVGPNRVMIGDDTLTARQIVIAAGAHPRPLGVPGADLVTSSDDFFY
                     LETLPDRIVFIGGGYISFELAHVAARAGSEVTILQRSGRVLKGFDPDIVDRLVLASRE
                     IGIDVQVNMPLVSVERTTAGLLVRAGKKGEESTFEADMVVHGAGRVSTTEGLGLAAGN
                     VATDRRGIVVDDHLRSVSNPAVSVAGDANPTSPQLTPVAVMDAHIVVDNILNGNTRIA
                     DYSVVPSAVFTAPPLASVGLTEEAAQKKGISYVVTAGDLSARFTIRSIGQKHAGYKLL
                     VEKDSRRILGAHLIGPHVEEVINIFALAIKHNLTVDDLTLDAIPWAYPSSTYEVIHMI
                     HPLVRK"
     gene            complement(1730..2431)
                     /locus_tag="PLV90_04035"
     CDS             complement(1730..2431)
                     /locus_tag="PLV90_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOF96314.1"
                     /translation="MLSFDEEIDALEQERELLLRFPEEEFIAIIRDVGFSCDCCGRCC
                     TREFNGHVFLLEDDTDRVLSFQPDALMPAPDFDACDQKGRFYVSGYALRTKPDGSCIF
                     LEDGRCSIYDRRFSICRVYPYMLHREADERGVVDWRQIGGLNEHGCYNTPIDGAECAR
                     IARETRAYEAAFLDQEIRFKRALRDLFARDGLRYVRRTYDLLMRDFRKGKEVEVRVFH
                     RGRFYPHRVACDSYE"
     gene            2696..3631
                     /locus_tag="PLV90_04040"
     CDS             2696..3631
                     /locus_tag="PLV90_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HOF96315.1"
                     /translation="MIITVIILVVGIAILVKGADLFVSGGSGLALHHRISPALIGSTI
                     IAFGTSLPELVVSTDAAATGNTGIALGNVLGSNIANIALVLALCTLIRPGMVAASGTS
                     RSALVRHTALMLGATAAFTLLAMRGTLDALTGVVLLILFAIILYLLLRERREESVPDI
                     KSHGWADALYIGLGLVAVIVGARLVVDSAVSLADAFGIPAFVIGLSIVAVGTSLPELA
                     TSLVAAIKNEGAISIGNILGSNIFNLLLVLGISLLLAPVAIGSFADIIAVALFSVAIL
                     PLLFARPSVVRAWSIVLLIGYAAYIAWIFSTAPVG"
     gene            3725..5614
                     /locus_tag="PLV90_04045"
     CDS             3725..5614
                     /locus_tag="PLV90_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HOF96316.1"
                     /translation="MENWRSVAAAALGCLVVAAIIGTALASDEAGTDTLGDLQKFGSK
                     EEIEVFLKENTQKGWNGYPGAPGGAGRETGMNAPAPMATAAPTSSTRDYSTTNVQVEG
                     VDEADFVKNDGKYIYILSGGTLAIVEAFPAKDAKILSETRIEGTPEALFLAGDRLVVF
                     AVATEEEMTTPAGSVTPVPISRTVTHAHVYDIRDRSGPELIRTLTFTGDYYDARMVGE
                     YVYALTRESPLWVRDDLALPAVREGEAPPIRPEIYRPKTPMQNYVFYTASAFSVRGKG
                     APDAETFLLGYDTTLFASQENLYIGYRNMGPAYPAGGTPERTIIHRFAIGQGKIGYRA
                     MGEVPGHLLNQFSLDEHEKNLRVATTVEGWMWEGSIQYNNVYVLNSAMETIGTLEHIA
                     PGERIYAARFVGDRLYLVTFKQIDPLFVIDLSDPKHPGILGELKIPGYSDYLHPYDAD
                     HIIGIGKETDENGWGGVSVAGLKIALFDVSNVNKPIQVDAVVIGEAGTDSAALHDHKA
                     FLFAKEKGLLVVPVSEIVRVENPASKYPGSYGTATWQGAYVYRVSPASGFTLEGKVTH
                     AEKGPYPIWDASDAVRRSLYMDDTLYTVSARSIVMTGLGDGKRMNEVFLPYREGASPP
                     PYPVW"
     gene            complement(5572..8328)
                     /locus_tag="PLV90_04050"
     CDS             complement(5572..8328)
                     /locus_tag="PLV90_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOF96317.1"
                     /translation="MVPLLSPAESRATAWHALSAAEVAGRFDSPEGGLSGTEVARRRE
                     IFGENALPTKHPPAVIEIFLRQFASPLIYVLLAAGVVSVFFADAADAVFIFAVILLNA
                     VIGTSQEVKAERGATALQQMLRIRARVRRDGREATVPAENLVPGDRAILESGDRVPAD
                     IRVTRAQSLSIDESLLTGESHAVGKNPDAVDPSLPPADRLNMLYAGSTVMSGRAMGIV
                     VATARNTEIGQIAETVTASEAGKPPLTIRMEDFSRKISIAVLAATGLLAFIVLGQGMP
                     LLEVFFLAIALAVSAIPEGLPVALTVALSIATSRMAGRNVVVRYLAAVESLGSCTLIA
                     SDKTGTLTVNQQTARVVALPTGEVFEVTGAGYSGEGEVVDESGTPVAGAPRAHLEHLA
                     RAAAINNEATLERNGGGDWAHRGDAIDVAFLALTYKLGLDPAGIREDAPPVAGLPFES
                     ERRYAAVWYREEEEILVAVKGAAETVVPFCRTMAGGSGGNVPLDPDRVQKGHDNLTSR
                     GFRVLAVAHGRVEGTFSAGEPELPPLELLGLVGFIDPIRPDVPDAVRRCRSAGVDVVM
                     VTGDHPATALAIARELRITDSHDEVVTGAELDDEETSTSLRFIDRVKHARVFARVTPL
                     QKLRIVEAYRESGAFVAVTGDGVNDAPALRSANIGIAMGSGTDVARDTASLIITDDNF
                     ASIVAGIEEGRYAYDNVRKVVYLLISTGFAEVLLFMLALAMGLPLPLIAVQLLWLNLV
                     TNGIQDVALAFEGGEPGVMDRPPRRPEEGIFNRLMMEEVLLSGTVIALVTLGVWYWLL
                     SNGWDEFAARNLLVLLLVLFENFHVFNCRSEHLSAFRIPISNNYFLIAGVIAAQGIHI
                     LALYTPVLRDVLQVQPVSLVEWLSLLVLASSVVVVMEVFKAVRKRRPSGHQTGYGGGE
                     APSR"
     gene            complement(8514..8846)
                     /locus_tag="PLV90_04055"
     CDS             complement(8514..8846)
                     /locus_tag="PLV90_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOF96318.1"
                     /translation="MSKAKENLAEQVIDLCNLVAYQSGSIVSRMLVYTKAGTITVFAF
                     DAGEGLSEHTAPYDAVLQVLDGEALITIADKEYPMKAGELIIMPAKIPHAVHATTPFK
                     MMLTMIHA"
     gene            8979..10097
                     /locus_tag="PLV90_04060"
     CDS             8979..10097
                     /locus_tag="PLV90_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04084 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HOF96319.1"
                     /translation="MYYHLILTDNCNLCCTYCRGKAFTVDPPELPDITVDEDLPVDLD
                     IDLSDLYRFLAKDPDAVLTFYGGEPLLAADRICEIARDAPVSALILHTNGTLLDRLEP
                     RVANRFDTILVSIDGPEALTDAHRGEGTFGLITKNLRNLVAGGFAGELIARMTVTEDT
                     DIVEAVSYLAENDRFSFPAIHWQMDANFWNDYSLRDYAAWVTESYNPGIRSLVALWVE
                     TMREEGRVLRWYPFMDPMQDMLLSRPSMLRCGCGHANYSIMTDGQIAPCPIMVGMRDY
                     YVGHIATADPLRLPVTTVGSPCTECRLLDFCGGRCLYSNITRPWPEAGRRVVCETVEN
                     LYQALSAALPEVRALIGAGRVGMEDFNHRKYNSCEIIP"
     gene            10094..10255
                     /locus_tag="PLV90_04065"
     CDS             10094..10255
                     /locus_tag="PLV90_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96320.1"
                     /translation="MTANSLLCRIGLHRWGKKRGYDQYSSNVIEWEQRCERCGKRKRW
                     VEAKRDRRR"
     gene            complement(10284..10859)
                     /locus_tag="PLV90_04070"
     CDS             complement(10284..10859)
                     /locus_tag="PLV90_04070"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOF96321.1"
                     /translation="MTKRSIGPRTLLYPHPVLIIGTYDVSGRPNAMAAAWGGICSSRP
                     PAVAISVQKARQTYENLVEQREFTISIPPVDYVREADYFGIVSGHDTDKFAATGLTPV
                     KADRVNAPYVGEFPVVLECRLLQTVEIGVHTQFIGEILDVKVDESVIGPDGKPDIGKI
                     RPFAYDSMRQEYYGFAGVVAKAFSAGKEVKR"
     gene            10937..11230
                     /locus_tag="PLV90_04075"
     CDS             10937..11230
                     /locus_tag="PLV90_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96322.1"
                     /translation="MHRPVEKVASFAATTARSLEIVANSAPMGSGDGVVARTISRGRS
                     PPTKKTATIGPPVARAPALSMLEAISKTERPATVRRMGSRSIIPDVQPLADRI"
     gene            11308..11760
                     /locus_tag="PLV90_04080"
     CDS             11308..11760
                     /locus_tag="PLV90_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOF96323.1"
                     /translation="MGLVKCCREQVVAVSPDTPAVEVAKIMGEKNVGSVVIVTGDNRP
                     TGVITDRDLTIRVMAHEKNPGEVRAGDITTRDVITFRDSMGIYEAIQKMTAEGIRRMP
                     VVDDAGELIGIVTMDDIVRMLGEEMAAIAKNIEKQSPPLQREAPPIPM"
     gene            complement(11800..12255)
                     /locus_tag="PLV90_04085"
     CDS             complement(11800..12255)
                     /locus_tag="PLV90_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HOF96324.1"
                     /translation="MPKLTVKLDFEDFPDVYTCKGANRSPPIRVEGILPNIKSLAVLA
                     TTSPEEGPSKVAWILWNLPAVPLIPEGFPAGGVVESPLHAVQGTNDFGTVGYRGPCPE
                     TGKGEAYLFRVYALDLDLALAPGANWEEMIRKMQGSLNQSGEAIAFAVR"
     gene            complement(12473..12961)
                     /locus_tag="PLV90_04090"
     CDS             complement(12473..12961)
                     /locus_tag="PLV90_04090"
                     /inference="COORDINATES: protein motif:HMM:NF013340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HOF96325.1"
                     /translation="MSMQDLKVEIDFDHFPPEYTCDGEGISPRIRITGSGAPYLAIII
                     DDPDASKGTFTHWVAWNIPSTEEIPPGIPPEPRISHPVSIVQGMNDSRKTGYTGPCPS
                     KGVHRYFIRVWGVERELDLPPGSERVELTDALASAATGYGEAMATYGREKVLKSPPAT
                     RT"
     gene            complement(13139..13843)
                     /locus_tag="PLV90_04095"
     CDS             complement(13139..13843)
                     /locus_tag="PLV90_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOF96326.1"
                     /translation="MLDTGRIIEDKTMRGRLSVFADRTDAGRRLAATLSQLETVAEPV
                     VCAIPAGGVPPGLEVARALQAPLRMAVVRKVQIPWNPEAGFGAVTWDGRVFLNRALMA
                     GLGLSEAEVGAAVAKARKSVEERVEMFSGGRPEPGLFGRTAILVDDGLATGYTMLAAV
                     EAVRAGSPREVIVAVPTGSLHAVEFIARKADLVVCPNIRTGRTFAVAQAYERWHDITD
                     REVQELLIQARDMGLF"
     gene            13949..14392
                     /locus_tag="PLV90_04100"
     CDS             13949..14392
                     /locus_tag="PLV90_04100"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HOF96327.1"
                     /translation="MTQKETGGGTGEVTLYTDGASRGNPGDAAWAYVIVRDGAVAASS
                     SGFIGKATNNVAEYHAVINGLRAAREFTRGHLEVRSDSELVVRQLTGRYRITKEHLAA
                     LADEVRQWSRHFTKVRFENVPREDPCIQVADRLCNEVLDAEGRRR"
     gene            14395..14832
                     /gene="tsaA"
                     /locus_tag="PLV90_04105"
     CDS             14395..14832
                     /gene="tsaA"
                     /locus_tag="PLV90_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOF96328.1"
                     /translation="MAPIECTPIGVVRSPYRARGDAPRQGRLADTVAEIHVFDAYAPG
                     LEDVERSSHLIVLYWLDRAERGLLYAKPPGETRARGVFSTRSPARPNPIGFGIVDLVR
                     RDGDILVVRGLDALDGTPVLDIKPYSPEIDCIPEATGGWHIKK"
     gene            15211..16113
                     /locus_tag="PLV90_04110"
     CDS             15211..16113
                     /locus_tag="PLV90_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOF96329.1"
                     /translation="MTQTNEPNKDTCSGDCAGCPSATKCDDQRNVGAPKGLPPKADVM
                     VKHVVLVLSGKGGVGKSTVAANLAYALANQGYNTGLIDLDIHGPDIPKMLGIEDARLQ
                     SYDGKVIEPVRVTGNLAIISMAFLLPERNTPVIWRGPMKMTVIKQFLEDVNWGSLDYL
                     VVDLPPGTGDEALTVAQLAPNIAGAVIVTTPQDVAILDSSKAVQFIKKLELPVLGIVE
                     NMSGFICPHCKEEIDIFGKGGGEKEAKELGVPFLGAIPLDPDMRKAADEGRPFIIRQA
                     GSAENPTWKSIDAVMKALVKQIEE"
     gene            16117..>16430
                     /locus_tag="PLV90_04115"
     CDS             16117..>16430
                     /locus_tag="PLV90_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96330.1"
                     /translation="MEYARVLSGREDPLPIFSKVVEALDNFEEFPFLLEPIYREASDL
                     GDEDLDRFRFGLVRLQVYADIHRYEDMEAAQRMKYVAATIERVLFGRLLLEGEEAGGK
                     QC"
BASE COUNT         3067 a         5306 c         5265 g         2792 t
ORIGIN      
        1 ccgcggcggg ggtgtactga cccacgcagg ggatccctgg agaaggagag cgcggtgggg
       61 agcatccgga gccgcaggag tctccggcac atggacccgc atgtggccgg ccccgggccg
      121 gggaactcat cactttcgca caaggggatg gatcatgtgg atgacctcgt aggtgctgct
      181 cgggtaggcc cacgggatgg catcgagcgt gaggtcgtcc accgtcaggt tgtgcttgat
      241 cgcgagggcg aatatgttga tcacctcctc gacgtgcggc ccgatcaggt gggccccgag
      301 tatccggcgg gagtccttct cgaccaggag tttgtagccg gcgtgcttct ggccgatgct
      361 ccggattgtg aaacgcgccg agaggtcgcc ggcggtgacg acgtacgata tccccttctt
      421 ctgcgccgcc tcttccgtga gcccgaccga ggcgaggggc ggggccgtga agacggcgct
      481 cgggacgacc gagtagtccg cgatccgggt gttgccgttc aatatgttgt cgacgacgat
      541 atgggcatcc atcaccgcca ccggcgtcaa ctgtgggctg gtggggttcg catctccggc
      601 aacggagacg gcgggatttg agacgctccg gaggtggtcg tcgacgacga tcccccggcg
      661 gtcggtcgcg acgtttcctg ccgcaaggcc gagaccttcg gtcgtagaga cccggccggc
      721 gccgtggacg accatatcgg cctcgaaagt actctcttcg cctttctttc cggcccgcac
      781 cagaagaccg gcggtggttc tctcgactga gacgaggggc atgttcacct ggacgtcaat
      841 cccgatctcc cttgaggcca gcaccaaccg gtcgacgata tcgggatcga accctttcaa
      901 gacccgtccg ctccgctgga ggatggtcac ttccgacccg gccctggcgg ccacatgggc
      961 gagttcaaag gagatgtatc ctccgccgat gaagacgata cggtcgggaa gcgtctcaag
     1021 gtagaagaag tcgtcgcttg acgtcacgag atccgcaccc gggacaccga gcggccgggg
     1081 atgtgcgccg gcagcgatga cgatctgacg ggccgtgagc gtatcgtcgc cgatcatcac
     1141 ccggttcggg ccgacaaagc gggcagattc gtggtaagta tcgacccctg cctcccggag
     1201 actctcctcc ttccgtcggg gggtgggacc ggtgaacgtg cgcacaaagg cgatgagagc
     1261 cggccagtcg atcgagacgg ttccgcggac cccgttcccc tgctgatcat gaacgcgtgc
     1321 aaccacctcg gcagccccgg cgagcacctt ctttgggaca caaccccgga gagcgcacgt
     1381 ccccccatac tcccgccggt cggtgatcgc cacccgcata ccggcatctc cgcagtgcca
     1441 ggcgacatca gaaccggcga ccccggtccc gatgacgacg acatcgtact cccgctccat
     1501 aatggggccc cagtcaccac agagcggtat aaaggtttac ccggatcacg ggagaggaga
     1561 gcccgtcagg ccgcaggtaa aacagctccc gctctccctg gagagagagc gaagggcacg
     1621 atcattgcga cgacagttgc cgcacggaga atcggagaag acccggatag ggatgaccat
     1681 gccgggcctg gcggcaccgc ggggaccccg gcctccgccg gcgattccat cattcgtaac
     1741 tatcgcaggc gacccggtgc gggtagaacc gcccgcggtg aaagaccctg acctcgactt
     1801 cctttccctt ccggaaatcc cgcatcagga ggtcgtaggt ccgccggacg tagcgcaacc
     1861 catcacgcgc aaagaggtca cggagggctc ttttgaaccg gatctcctgg tcgaggaacg
     1921 ccgcctcgta tgcccgggtc tcccgggcta tccgggcgca ttcggcgcca tcgatcgggg
     1981 tgttgtagca cccgtgctcg ttcaggccgc cgatctgccg ccagtcgacg acgccccgct
     2041 catcggcctc ccggtggagc atgtagggat agacccggca gatcgagaac cgccggtcgt
     2101 agatgctgca cctgccatcc tcaaggaaga tgcaggaacc gtccggcttt gtccggagtg
     2161 cgtagccgga gacatagaac cgtcccttct ggtcgcaggc atcgaagtcc ggcgccggca
     2221 tgagggcatc gggctgaaac gataggaccc ggtcggtgtc atcctcgagc agaaagacgt
     2281 ggccgttgaa ctcccgggtg cagcaccggc cgcagcagtc gcaggagaac cccacgtcgc
     2341 ggatgatagc gatgaactcc tcctcaggga accggagaag cagctcgcgt tcctgctcga
     2401 gggcatctat ctcttcgtca aacgagagca tacggcagca acctccccat cgtgagcccg
     2461 gcaggtgtgc cgggactgat accctcaagt attcgacggc atggcggata tctcctccgg
     2521 caccccgggg tgcaccggag gggcaggcat cctgtgcggc gccacgggcg gcagcgggga
     2581 ggcaccatcg tctcttcgcc ggcacccggc ccgggagagg ctgtagaggg cccggatcgg
     2641 gaaaagtatt aggagaactc cgcccaagta ctaccactac atccggggcg ctcccatgat
     2701 aataaccgtc attatactcg tcgtcggcat cgccatcctc gtcaaaggag ccgatctctt
     2761 cgtgagcggt gggagcggtc ttgctctcca ccaccgcata tccccggccc tgataggatc
     2821 cacgatcatc gcgttcggca catcgctccc ggagctcgtc gtcagcacgg atgcggcagc
     2881 cacggggaac acggggattg ccctcggaaa tgtcctcggg agcaacatag caaacatcgc
     2941 cctcgtcctc gcgctctgca ccctcatccg gcccggcatg gttgccgcgt cggggacgtc
     3001 acgctcagcg ctcgtccggc acaccgccct gatgctcggt gcgacggcgg ctttcaccct
     3061 cctcgctatg agaggtacgc tcgacgccct gacaggggtt gtactcctca tcctctttgc
     3121 gatcatcctc taccttctcc tgcgggagcg ccgtgaagaa agcgttcccg atatcaagtc
     3181 tcacgggtgg gccgacgcgc tctacatcgg cctcggcctc gtcgccgtca tcgtcggcgc
     3241 ccgactggtc gtcgacagtg ctgtctcact cgcggatgcc ttcgggatcc cggcattcgt
     3301 catcggcctc tcgatcgtcg ccgtcggcac gtcgctcccg gagctcgcga catccctcgt
     3361 ggctgctatc aagaacgaag gcgccatatc gatcggcaac atcctcggga gcaatatatt
     3421 caaccttctt ctggttctcg ggataagcct cctcctcgcc ccggtcgcca tcgggtcgtt
     3481 tgccgatatc atcgccgtcg ccctcttctc ggtcgcgatc ctccccctgc tctttgcccg
     3541 gccgtccgtc gtcagagcct ggtcaattgt cctgctcatc ggctacgcgg cctacatcgc
     3601 ctggatcttc tcgacggcgc cggtcgggtg agagtgttct caacgaggct tcgaaaatat
     3661 tcgtaggtta cacaaacctg atgctccggc gcgcccatct tccttcatcg gaaggtgaac
     3721 gagtatggaa aactggagat cggttgcggc agccgccctc ggctgtctcg tggtcgcagc
     3781 gatcatcggg accgcccttg cgtcggatga ggccgggacc gataccctgg gcgacctgca
     3841 aaagttcggg tcgaaagagg agatcgaagt gttcttgaag gagaacacgc agaaagggtg
     3901 gaacggctac cccggggccc cggggggtgc cggccgggag accgggatga acgccccggc
     3961 gccgatggcg accgccgccc cgacgtcttc tacccgggac tactccacga cgaacgtgca
     4021 ggtggagggc gtggatgagg ccgacttcgt gaagaacgat gggaagtaca tctacatcct
     4081 ctcgggcgga accctcgcga tcgtcgaggc gttcccggca aaagatgcga agatcctatc
     4141 cgagacccgg atcgaaggca ccccggaggc actcttcctc gcgggcgacc gtctggtggt
     4201 ttttgccgtc gcaactgaag aagagatgac cacaccggca ggaagcgtga cgccggtccc
     4261 catctcgcgg acggtgacgc acgcacacgt ctacgatatc cgtgatcggt ccggcccgga
     4321 gctgatccgg accctgacct tcaccggcga ctactacgac gccaggatgg tcggggagta
     4381 cgtctacgcc ctcacgcgag agtcgcccct ctgggttcgg gacgacctcg ccctccccgc
     4441 ggtgagggag ggtgaagcgc cgcccatccg acccgagatc taccgcccga agactcccat
     4501 gcagaactac gtcttctaca cagcaagcgc cttttccgtc agggggaagg gcgcccccga
     4561 tgccgagacg ttcctcctcg gctacgacac cactctcttt gcgtcgcagg agaacctcta
     4621 catcggctac cggaacatgg ggccggcata tcccgccggc ggcacccccg agcggacgat
     4681 catccaccgg ttcgcgatcg ggcaggggaa gatcggctac cgtgcgatgg gcgaggttcc
     4741 cgggcacctc ctgaaccagt tttcgctcga cgagcacgaa aagaacctcc gggtagcgac
     4801 gaccgtcgag ggctggatgt gggagggctc gatccagtac aacaacgtct acgtcctcaa
     4861 cagcgcgatg gagaccatcg ggacgctcga acatatcgcg ccgggggaac ggatatacgc
     4921 cgcccggttc gtcggcgacc ggctgtacct cgtgacgttc aagcagatcg accccctctt
     4981 cgtcatcgat ctctcggacc cgaagcaccc gggcatcctc ggggaactca agatccccgg
     5041 gtactccgac tacctccatc cctatgacgc cgaccacatc atcggtatcg ggaaggagac
     5101 ggacgagaac ggctggggcg gcgtctcggt cgcgggtctc aagatcgccc tcttcgacgt
     5161 ctcgaacgtg aacaagccca tccaggtcga cgccgtcgtt atcggcgagg ccgggaccga
     5221 ctccgcggcg ctccacgatc acaaggcctt cctctttgca aaggagaaag gtctcctcgt
     5281 cgtcccggtg agcgagatag tgcgggtgga aaaccccgca tcgaagtacc ccggctctta
     5341 cggcaccgca acatggcagg gggcctacgt ctaccgggtg agccccgcct ccgggttcac
     5401 cctcgaagga aaggtcaccc acgccgagaa ggggccgtac cccatctggg atgcatccga
     5461 cgcggtgcgc cggtcgctct acatggacga caccctctac accgtctcgg cgcgaagcat
     5521 cgtcatgacg gggcttggcg acggtaaacg gatgaacgag gtcttcctcc cctaccggga
     5581 gggggcctcc ccgcccccgt acccggtctg gtgaccggaa ggacggcgtt tcctgaccgc
     5641 cttgaagacc tccataacga ccacgaccga ggaggcaagg acgaggagcg agagccactc
     5701 gaccagtgaa acgggctgca cctggagcac atcccggagg acgggcgtgt agagagcgag
     5761 gatatggata ccctgcgcgg ctatgacgcc ggcgatcagg aagtagttgt tgctgatagg
     5821 gatgcggaac gcagagaggt gttcggaacg gcagttgaag acgtggaagt tctcaaagag
     5881 caccaaaagc aggacgagca ggttccgggc cgcgaactcg tcccacccgt tcgagagaag
     5941 ccagtaccag acaccgagcg tgacgagcgc gatgaccgtg ccggagagca gcacctcctc
     6001 catcatcaat cgattgaaga tcccctcctc cggcctccgc ggcggccgat ccatgacgcc
     6061 gggctcgccc ccttcaaatg ccagcgccac gtcctgaatg ccgttcgtca ccaggttcag
     6121 ccagaggagc tggacggcta tgagggggag cggcaggccc atggcaaggg cgagcataaa
     6181 gaggaggact tctgcaaacc cggtcgatat caggagatag acgactttcc ggacgttatc
     6241 gtaggcgtag cggccctctt ctatcccggc gacgatcgag gcgaagttat cgtcggtgat
     6301 gatcagcgat gccgtatccc gggcaacgtc ggtcccggag cccatcgcga tcccgatgtt
     6361 cgcgctccgg agcgccggcg catcgttgac cccatccccg gtgaccgcga cgaacgcccc
     6421 gctctcacgg taggcctcga cgatccggag tttctgcagc ggcgtcaccc gggcaaagac
     6481 ccgggcgtgc ttcacccggt cgatgaaccg gagggaagtc gaggtctctt cgtcatccag
     6541 ttccgcgccg gtgacgacct cgtcgtggga atccgtgatc cggagttccc gtgcgatggc
     6601 gagagccgtt gccggatggt ccccggtcac catcaccacg tcgactccgg cgctgcggca
     6661 tcgccgtacg gcatcgggaa cgtcgggccg gatcgggtcg atgaatccga cgaggccaag
     6721 gagctcgagc ggcggcagtt ccggctctcc tgcagagaat gtgccttcga cgcggccatg
     6781 agccacggca aggacccgaa agccccggga ggtgaggttg tcgtgccctt tttgcacgcg
     6841 atccgggtcg agcggtacat tgccgcccga tccgccggcc atggtgcggc agaacggaac
     6901 gaccgtctcg gcggcccctt tgaccgccac caggatctct tcttcctccc ggtaccagac
     6961 ggcggcatac cgccgctcgg actcgaaggg gagtccggca acaggaggcg catcttcgcg
     7021 gatgccggca ggatccagac ccagtttgta ggtgagcgcg aggaacgcca catcgatcgc
     7081 atcgccccgg tgcgcccagt ctcctccacc gttccgttcc agggttgcct cgttgttgat
     7141 cgctgcggcc cgggccaggt gttccagatg ggcacggggg gcaccggcca ccggggtgcc
     7201 gctctcatca accacctctc cttcgcccga gtaccccgcc ccggtgacct cgaagacctc
     7261 gccggtgggc agggcaacga cccgggcggt ctgctggttc accgtgaggg tcccggtctt
     7321 atcgctcgct atcagggtgc agctcccgag gctctccacg gcggcgaggt accggacgac
     7381 gacgtttcgc ccggccatac gtgacgtcgc aatcgagagc gcgacggtca gagcgaccgg
     7441 gaggccctcc gggatcgccg agaccgcaag cgcgatggcg aggaagaaga cctcgagcag
     7501 gggcatcccc tgaccgagga caatgaacgc gagcagtccc gtagcggcaa ggacggcgat
     7561 gctgatcttt cgggagaaat cctccatccg gatggtgagc gggggtttgc ccgcctccga
     7621 cgccgtgacc gtctcagcga tctgcccgat ctcggtattc cgggctgtgg caaccacgat
     7681 tcccatggcc cgaccgctca tgacggtgct gccggcatag agcatgttca ggcggtcggc
     7741 cgggggcagc gaggggtcga ccgcatcggg gttcttcccg accgcatggg attcaccggt
     7801 caggagcgac tcatcgatgg agagcgattg cgctctcgta acacggatat cggcgggaac
     7861 acggtcgccg gattccagga tggcgcggtc gccgggaacc aggttctcgg ccgggacagt
     7921 cgcctcacga ccgtctcgcc tcacccgagc ccggatccgg agcatctgct gcagcgccgt
     7981 tgcgccccgc tccgccttca cttcctggga ggtcccgatg acggcgttga gcaggatgac
     8041 cgcgaagatg aagaccgcat cggcggcatc ggcaaagaag acggatacga cccctgccgc
     8101 gaggaggaca taaatgagcg ggcttgcgaa ctggcgcaga aatatctcga tgaccgcagg
     8161 agggtgcttt gtggggaggg cgttctcacc gaagatctct cgccgtcggg ctacttcagt
     8221 gccggagagg cctccctccg gcgaatcgaa ccgcccggct acctcggcgg ccgaaagggc
     8281 atgccatgcc gtcgcccggg attcggccgg ggagaggaga ggaaccatgc cgtcgcctca
     8341 cgaggatacg ccgcagggta caggtgcagc cttcgggatc ggttcgagca ggctacgcgg
     8401 ttctccaggg cgaggccggc gataccactt ccagagaata tgcacggaag gagatccatc
     8461 accggcgcat aaatagttgc aggcggtgac cggcaacccc tcctcccggc gggtcaggcg
     8521 tgaatcatcg tcagcatcat cttgaacggc gtggtggcgt ggaccgcgtg gggaatcttc
     8581 gccggcatga tgatcagttc acccgccttc atgggatact ccttgtccgc gatcgtgatg
     8641 agcgcctccc cgtcgaggac ctggagaacg gcgtcgtaag gtgcggtgtg ctccgagagc
     8701 ccttcgcccg cgtcgaacgc aaagaccgtg atggtgccgg ccttcgtgta gacgagcatc
     8761 cggctgacga tcgagcccga ctggtaggcg acgaggttgc agaggtcgat gacctgttcg
     8821 gcaaggttct cttttgcttt ggacatacga gtccatttat cctgtgggaa cttctgtttt
     8881 gcgccgggtg tggtgggggc tcgccgccgg tgtggcgccg ggatgccccg tatccttatc
     8941 tttttcccgc cctgccccca acctgtaaga gaccttcagt gtactaccac ctcatcctga
     9001 ccgataactg caacctctgc tgcacctact gccgcgggaa ggcgttcacc gtcgaccccc
     9061 cggaactgcc cgatatcacc gtcgacgagg atctcccggt ggacctcgat atcgatcttt
     9121 ccgacctcta ccggttcctc gcaaaggacc ccgacgccgt cctgaccttc tacggcggag
     9181 agcccctgct cgccgccgat cgcatatgcg agatcgcccg cgacgccccg gtatcggcgc
     9241 tgatcctgca cacgaacggc accctcctcg accgcctcga gccccgggtc gcgaaccggt
     9301 tcgataccat cctggtctcg atcgacggcc ccgaggccct caccgacgct caccgcggag
     9361 aggggacgtt cgggctgatc acgaagaatc tccggaatct tgttgcgggg gggtttgccg
     9421 gcgagctgat cgcccggatg accgtgaccg aggataccga tatcgtcgag gccgtcagct
     9481 accttgcgga gaacgaccgg ttctcgtttc ctgccatcca ctggcagatg gacgcaaact
     9541 tctggaacga ttactccctg cgcgattacg ccgcatgggt gacggagagt tacaaccccg
     9601 gcatcagatc gctcgtcgcg ctctgggtcg agacgatgcg ggaggagggc cgggtgctcc
     9661 ggtggtatcc cttcatggac ccgatgcagg atatgctcct ctcccggccg agcatgctcc
     9721 ggtgcgggtg cggccacgcg aactacagca tcatgaccga cggacagatc gcaccctgtc
     9781 ccatcatggt cggtatgagg gactactacg tcgggcatat cgccacggcc gaccccctcc
     9841 gcctcccggt gacgaccgtg gggagcccct gcacggagtg ccggctcctc gacttctgcg
     9901 gcggccggtg cctctactcg aatatcaccc gcccctggcc ggaagccggg cggcgggtcg
     9961 tctgcgagac ggtggagaac ctctatcaag cgctctcggc ggcactcccg gaggtcaggg
    10021 ccctcatcgg cgcggggcgg gtcgggatgg aggactttaa tcaccggaag tacaacagct
    10081 gcgagatcat accatgacgg caaacagcct cctctgcagg atcgggcttc accgctgggg
    10141 taaaaagcgc ggatacgacc agtactcttc aaacgtcatc gagtgggagc agcggtgcga
    10201 acggtgcggg aagcggaagc ggtgggtcga ggcgaaacgg gaccgccggc ggtgacgggg
    10261 ataccgcacg gatacaaaaa gagttacctt ttgacctcct tccccgccga gaaggccttc
    10321 gcgacgacgc cggcaaagcc gtagtactcc tgccgcatcg agtcgtaggc gaacggccgg
    10381 atctttccga tatcgggttt gccgtccggc ccgatgacgc tctcgtccac cttcacgtcc
    10441 aggatctcgc cgatgaactg ggtgtgcacc ccgatctcga ccgtctggag gagccggcac
    10501 tcgaggacga ccgggaactc ccctacgtag ggagcgttca cgcggtcggc cttcaccggc
    10561 gtaagcccgg tcgccgcgaa tttatccgta tcgtggccgg agacgatgcc gaagtagtcg
    10621 gcctccctga cgtagtccac cgggggtatg ctgatcgtga actcgcgctg ctcgaccagg
    10681 ttctcgtagg tctgccgggc cttctggacc gatatcgcga ccgccggggg gcgggatgag
    10741 cagataccac cccatgcggc ggccatggca ttcggccggc cggagacgtc gtaggtcccg
    10801 atgatcaaga ccgggtgagg gtagaggagg gtccgtggac cgatcgatcg cttcgtcata
    10861 ggataacggt tcgcccctgg accgggataa acctttttga cccgggtcat gcagcgcccg
    10921 cccccctccc ccggaggtgc accgaccggt agagaaagta gcctctttcg cggccacgac
    10981 cgcgaggtcg cttgagatcg tcgcgaacag tgcgccgatg gggtcaggcg acggcgtcgt
    11041 cgcccggacg atcagccgcg ggagaagccc tccgaccaaa aagaccgcaa cgatcgggcc
    11101 ccccgtcgcg agggcgccgg cgttatcgat gctcgaggcg atctcaaaga cggagagacc
    11161 cgctacggtc agaaggatgg gcagccggtc catcatcccc gatgtacagc ctctcgccga
    11221 tcgcatatag ggggagaagg ccgggggtgt acgggttacg ttcatatatc ataccggcat
    11281 ggatgaggcg ccagaggagg taacgatatg ggacttgtga aatgctgccg tgagcaggtc
    11341 gtcgccgtct cgccggatac gccggcggta gaggtggcga agatcatggg agagaagaac
    11401 gtgggaagcg tcgtcatcgt caccggtgac aaccggccca caggggtgat caccgaccgc
    11461 gacctcacga tccgggtgat ggcgcacgag aagaacccgg gcgaggtccg ggcgggggat
    11521 atcacgaccc gggacgtgat cacgttccgg gacagcatgg ggatatacga ggccatccag
    11581 aagatgaccg ctgaaggcat ccggcgaatg ccggtcgtcg acgatgccgg ggaactcatc
    11641 gggatcgtca ccatggacga catcgtccgc atgctcgggg aggagatggc cgctatcgca
    11701 aagaatatcg agaagcagag tccgcccctg cagagagagg ccccgcccat acccatgtga
    11761 tggggccggc acccctgttc tggcgggaac cccccgcccc taccttacgg caaacgcgat
    11821 agcctccccg gactggttca gcgacccctg catctttcgt atcatctctt cccagttcgc
    11881 cccgggcgcg agcgcaaggt cgaggtcgag cgcgtagacc cggaagaggt aggcctcgcc
    11941 cttcccggtc tccgggcagg ggccccggta gccgacggtg ccgaagtcgt ttgtgccctg
    12001 gacggcatgg agcggcgact ccaccacccc gccggccggg aatccctccg ggatcagggg
    12061 gaccgccggg aggttccaga ggatccacgc caccttcgac ggcccctcct cggggctcgt
    12121 cgtcgcgagg accgcaagcg acttgatgtt cgggaggatc ccttccaccc ggatgggagg
    12181 ggaacggttc gcccccttgc aggtgtaaac gtcggggaaa tcttcaaagt ccagtttcac
    12241 ggtcagtttc ggcatacagc acgtctccgt tggtagagat ctgtcgggga cgtgaaaaag
    12301 gtttgtccga ccatggagag gaggaacccc tgcatggtat cgataagaat atatctgccg
    12361 gcgcccgccg cgggaacgag cgtgacgtgc gatccataac cctctcgccc gggggaggtg
    12421 gagaccgaac cgggcggagt cccgaaagat tggagggggg gtggaggaga tctcaggttc
    12481 gggtcgccgg cggggacttg agcaccttct cacggccgta agtcgccatc gcttcaccgt
    12541 agccggtggc ggcggaggcg agcgcatccg taagctctac ccgctcggat ccgggcggga
    12601 gatcgagctc tctctcgacg ccccagaccc gtatgaagta ccggtgcaca cccttcgagg
    12661 ggcaggggcc ggtgtaacct gtcttccggg agtcgttcat cccctggaca atggagaccg
    12721 gatgggatat ccggggctcc ggggggatgc ccggtgggat ctcctccgtc gacggtatat
    12781 tccaggcaac ccagtgcgtg aatgtgccct tcgaagcatc cggatcatct atgatgatcg
    12841 caaggtaggg cgcacctgat ccggtaatcc ttatccgcgg ggagatgccc tccccatcac
    12901 aggtatactc ggggggaaag tgatcaaaat cgatctcgac cttcaggtcc tgcatgctca
    12961 tcacctctcc ggcacccctg aggggaggtc gtatatacct gttgcggtac gggacatcgg
    13021 ggccggatcc caaggcccgg tgaacccgaa gcggcggcga cgtggctcac ccgcaagcag
    13081 gggcgccggc agcgggtggt gccggggccg tttcccggac cgttccggct ccgggttatc
    13141 aaaagagccc catatcccgg gcctggatca gcaactcctg cacctcccgg tcggtgatgt
    13201 cgtgccaccg ctcgtaggcc tgtgccaccg cgaaggtgcg gccggtcctt atgttcgggc
    13261 agacgacgag gtcggccttc cgggcgatga actcgacggc atggagggag cctgtgggga
    13321 ccgccacgat gacttcgcgg ggtgacccgg cccggaccgc ctcgacggcg gcaagcatcg
    13381 tgtaccctgt cgcgaggccg tcgtcgacca ggatcgcggt ccgaccgaaa agcccgggtt
    13441 cgggccgccc gccggagaac atctccacgc gctcctccac gctcttcctg gcctttgcga
    13501 cggcagcgcc cacttcagcc tccgagagac cgagcccggc catcagggcg cggttcagga
    13561 agacccgccc gtcccaggtc acggcaccga acccggcctc ggggttccag gggatctgca
    13621 ccttccgcac cactgccatc cggagcggtg cctggagcgc ccgtgccacc tcaagccccg
    13681 gcggcacccc gccggcgggg atggcacaga ccaccggttc ggcaaccgtc tcgagctgcg
    13741 agagcgttgc ggcgaggcgg cgcccggcat cggtccgatc cgcaaaaacg ctcaatcgac
    13801 cccgcatcgt tttatcctca atgatccggc ccgtatccag cataggtccg cccttaaccc
    13861 gccggggtat taacatttcg gggtggactt cccttcaccc cggaaagcga tatcaataac
    13921 cctccagaga tcgatgatcg atcggcagat gacacagaaa gagacgggag ggggcaccgg
    13981 tgaggtgacg ctgtacaccg acggtgcatc gcgggggaac cccggggatg ccgcctgggc
    14041 atacgtgatc gtgcgggacg gcgccgtcgc cgccagtagt tcggggttca tcgggaaggc
    14101 gaccaacaac gttgccgagt accacgccgt catcaacggc cttcgcgccg cacgggagtt
    14161 cacccggggg caccttgagg tccggtcgga ctcggagctg gtcgtccgcc agctcacggg
    14221 ccggtaccgc atcacgaagg agcacctggc ggcccttgcg gatgaggtgc ggcagtggtc
    14281 tcgccacttc acaaaggtca ggttcgagaa cgtcccgagg gaggacccct gcatccaggt
    14341 cgccgaccgc ctctgcaacg aggtgctcga tgcggagggg aggaggcggt agatatggcc
    14401 ccgatcgagt gcacccccat cggcgtcgtc cggtcgccct accgggccag gggtgacgcc
    14461 ccgcgccagg ggcgcctcgc ggataccgtc gcggagatcc acgtcttcga tgcttacgcc
    14521 ccggggcttg aggatgtaga gcggagcagc cacctcatcg tgctctactg gctcgaccgg
    14581 gcggagaggg ggctcctcta tgcaaagccg ccgggggaga cccgggcgag aggggtcttt
    14641 tccacccgct cgcccgcccg cccaaacccg atcggattcg ggatcgtcga cctggtccgg
    14701 cgcgacggcg atatcctggt cgtcaggggg cttgacgccc tggacggaac cccggtgctg
    14761 gacatcaagc cctactcgcc cgagatcgac tgcatacccg aggcgacggg cggatggcac
    14821 atcaagaaat aggccgggga agaggggccg atgccggggt cgcgggggtc cctatcgcgg
    14881 tcccattccg ggagctccgg tactgtacgg ccggcatcgg cgaggtgccc cgggggatgg
    14941 gcccggcgac gtcctctccg caccgccggg gtcttgagag gatggcagag gcagccaggc
    15001 gaatgtcgta agagacccct ggcgacggca acgttgccgc cctcctcccc ccccttgcgg
    15061 cgggcggata cactcgccga aaagcggtat tccccgaggc atacaggaaa ttttgccatc
    15121 acctatacct ttttgtgacg cttgccgcta atatatagta gctctagatc ttccttagcg
    15181 aaggatatca tatcgtggag gataattctg atgactcaaa cgaatgaacc caataaagat
    15241 acctgttccg gagactgcgc cggctgtccg tctgcgacca agtgcgatga ccagagaaac
    15301 gtgggcgctc caaagggcct ccccccgaag gccgatgtca tggtaaagca tgtcgtcctc
    15361 gtcctctccg ggaaaggcgg ggtcggcaag agcacggtag cggcaaacct tgcctacgcc
    15421 cttgccaacc agggctacaa cacgggcctc atcgaccttg acatccacgg cccggatata
    15481 ccgaagatgc tcggcatcga ggatgcccgt ctccagtcct acgacggaaa ggtcatcgag
    15541 ccggtcaggg tcaccggcaa cctcgcgatc atctccatgg cgtttttgct cccggagcgc
    15601 aacacccccg tgatctggcg cggcccgatg aagatgacgg tcatcaagca gttcctggag
    15661 gacgtcaact ggggcagcct cgactacctg gtcgtcgacc tcccgcccgg aaccggcgac
    15721 gaggcgctca ccgtcgccca gctcgccccg aacatcgcgg gggccgtcat cgtcaccacc
    15781 ccgcaggacg tcgcgatact cgactcgagc aaggccgtcc agttcatcaa gaaactcgag
    15841 ctcccggtcc tcggtatcgt ggagaacatg agcggcttta tctgccccca ctgcaaggag
    15901 gagatcgata tcttcggcaa aggcggcggc gaaaaagaag caaaggagct cggggttccc
    15961 ttcctcggcg ccatcccgct cgatcccgat atgcggaagg cggcggacga agggcggccc
    16021 ttcatcatcc gccaggccgg gagcgccgag aacccgacct ggaagagcat cgatgccgtc
    16081 atgaaagccc tggtcaagca gatcgaggag tgaccaatgg agtacgcgag ggttctttcc
    16141 gggcgagagg accccctccc aatattctca aaggttgtcg aggcgctcga taacttcgag
    16201 gagttcccct tcctgctcga accgatctac cgtgaggctt ccgaccttgg ggacgaggat
    16261 ctcgaccgct tccggttcgg cctcgtccgc ctgcaggtct acgcggacat ccaccgctac
    16321 gaggatatgg aggcggccca gcggatgaag tacgtggccg caacgatcga gcgagtgctc
    16381 ttcggccggc tcctcctcga gggagaagag gccggcggga agcagtgctg
//
LOCUS       DAOSII010000034        16407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_3709_length_16407_cov_49.485996, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000034 DAOSII010000000
VERSION     DAOSII010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 16407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16407
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3709_length_16407_cov_49.485996"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2381
                     /locus_tag="PLV90_04120"
     CDS             <1..2381
                     /locus_tag="PLV90_04120"
                     /inference="COORDINATES: protein motif:HMM:NF023252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3344 domain-containing protein"
                     /protein_id="HOF96331.1"
                     /translation="TFNRTFTLPAYTDIQWARLYTVVYAAGTDNRAGTVTVRFDGNGT
                     YATLGIETLATAGDNTANVYPVNDHVNRQYSDYLLWYDVTDLIGSEDPKAEVVATPVA
                     PNFDGRIKELVLVVAYNDGDGDQVHYWVNEGHDWQANGADGVTSTFATGGLGAGWTDA
                     TLRNVMLSSKDALYTFNGAPCTGADSSITFRANTWDVRTNLTAGSESRLTYVPNGGSY
                     KTTLATLAVRYPASVALPDLTVSTLAPNSGEVFSASENTYTATVTNIGAADAGAFAVG
                     FNASGAAGTAPVAGLAAGATATVTWTDETIRNAGDVVTITATADAANSVAEADEENNH
                     KTLVKTVVHNGYRGKRWTGGEDIATVKTYTVRGDLVYSSGNSTYLSASTYPNWAGYAV
                     SWTAGDLVVPANATVAAARLYVPYTWDKGPVFPSNVTLAFNGKTVDRAAFYADEKMWG
                     SSYPYGMTAYDVTDAFSVAGNTAVLTSTFPGGGNVSVRGMLLAVVYDDGRTAPHTVLV
                     NEGFDLLYGGAAQGTTPEQATAYAPFAIDAKDAVNARLVTVAPGAGPTKGDLLFNDRV
                     WTRVWNYTGTTQIGVDDRDVTALLAGENLAGFRSDADYMEAAAAFLVVEYPVPAGDTA
                     IILSPGWNFVSTPKRLADGANTFAIFDTVDTAAHSIQLYDGLERKWRQMAPTDPFRPL
                     DAVWVYANKTCTVPLTFAAGTPELPPAKDLGKGWNAIGFTDTVPETANYTLQSLGDHW
                     SILIGFDAGIQEYDTSIVRGATGPHGDERLMRPMQGYWLYVNAADTLGSLSG"
     gene            2392..2559
                     /locus_tag="PLV90_04125"
     CDS             2392..2559
                     /locus_tag="PLV90_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96332.1"
                     /translation="MRQGITLLLALVAGATAAGEVAPPAFPHEFYGSMTIDGATAPPP
                     LKSHDEDRDRR"
     gene            2870..4285
                     /locus_tag="PLV90_04130"
     CDS             2870..4285
                     /locus_tag="PLV90_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF96333.1"
                     /translation="MSDYQDKFSKIVDLLEERSPQGLSISAIARELGMNRASVSKYLE
                     MLQSSGDVSMQRFGRSKLYTPAHQVPFSELFDRLSSAIVILDADLRILMVNTSFIKAL
                     GIHRERNIIGAPLFDLNLRILSDPAIRRNIERIRAAGPYLAEMQHIEDSTNHIHLIEF
                     APIASRAGKPGIMISLRDITAWKNAEVALKNSEKKIRTIFETAPSGIVFFAVDGTILN
                     ANRASLRILGLKDFQELVNGNIFDISCYKGKILELIRQGKVAETELVCDFDLLKQEQR
                     IPSSRSGIAYFDVVFTPIALDGGGAPREFAILFWDVTTERLTRKELVFKETRYRSFFE
                     NTCNGVLIYEPVDDGNEYIFKDINHATEQILRMKKEDLIGRKLFEVFPDLPDPDVRNA
                     LIRVLKTEKPEFLLPLQYRKGKDSPWIWHYIFKLPSGEIASFMIDVSDAIREDAEGPL
                     RACGMHRQHSRDQPAPVSHAD"
     gene            4947..5939
                     /locus_tag="PLV90_04135"
     CDS             4947..5939
                     /locus_tag="PLV90_04135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HOF96334.1"
                     /translation="MKTQIGYFASLEQYRPMDALEQTIRAEKVGFDSIWADDHFHPWY
                     HDNAQSAQAWAWMGAALQATKRVFISTCITCPIIRYNPAIVAQTFATLRQMYPKRVGV
                     AVGAGEAMNEVPVTGVWPSVAERQDMTVEAIEVMRKLWESKEPVTFKGKFFTLDKAFM
                     YTKPDDEVPLYFSGLGPKGAKLAGMYGDHLMTVAAAPSVLKNVTIPKFEEGAREAGKD
                     PSKMERAMLIWYSVDPDYEKAVEGNRFWAGCLVPSMFKYKVYDPKEVQLHANLVHCDT
                     IKENYMCATDAEEMIKEIERFKAAGINHFCLGNSSPDVNTGIDIFKEIIPAVRD"
     gene            5980..6369
                     /locus_tag="PLV90_04140"
     CDS             5980..6369
                     /locus_tag="PLV90_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOF96335.1"
                     /translation="MENQSIARLMEEGAAFFSAGEAGRRLTWYTNPAWTGVSLADLAP
                     GADTKGSFTSHFVRIRKGCEVPDHLHESQWEWNVILDGHAKMVLDGREIPFKPGDTFT
                     TPPGVRHAVVAGKEDVALLAVFVPGPA"
     gene            complement(6553..7305)
                     /locus_tag="PLV90_04145"
     CDS             complement(6553..7305)
                     /locus_tag="PLV90_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOF96336.1"
                     /translation="MTRPCTIVISGKGGTGKTTIAALLIDALIAAGERPILAVDADPN
                     ANLHEALGIALTETLGGMREEAFTRAIPPGMDRTGYVKYRFRRALAEAEGYDLLAMGR
                     PEGSGCYCFPNALLTECIGTLERGYRFVVVDSEAGMEHIARGTIKNPDVLLIVSDPSA
                     RGIRTAGRIRDLAEGLGLSRDRVFLAVNRDRGDAVVESNLPLVARIPEDRGVGEADLA
                     GTPIVGIPQESPARAAVRMLAGWLIEECARRG"
     gene            complement(7302..8327)
                     /locus_tag="PLV90_04150"
     CDS             complement(7302..8327)
                     /locus_tag="PLV90_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96337.1"
                     /translation="MIPPPELLATGVGALPHRDPIEACRAVLAAFPRIPYVPTLPNRG
                     FLEAIVFADAEHLPGGVVRDGRLVIDRSTDPSEAMEQVLLDYLERNAEPYRVGKAYGS
                     GFHAMMGRDLSDALLVKCQVTGPVTFGMQVVDEARRPILYDPEYADLLGKLLGLRARW
                     CENAMRERMGARATLVVLSDPYLASLGSAVVPVDAGVAASAFEDIAALLDGGLGIHCC
                     ANTDWGFVLGLAPAVVSVDAWTYAREFLLYADEIARYMERGGVVAWGIVPADHAAFAA
                     EKPDAFFSRFEEIRARATETIDPDLFDRQSLITPTCGIRNANEEEAIEIMAATASLSR
                     RLRGEEP"
     gene            complement(8324..10258)
                     /locus_tag="PLV90_04155"
     CDS             complement(8324..10258)
                     /locus_tag="PLV90_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOF96338.1"
                     /translation="MEDVMPTVTFLPGYRKVTVPEGTTLLDAARAAGLSMNVVCGGQG
                     KCGKCLVFIKEGTVTFDREKYRRFFSDDEIARGACLACQATVVGDIQVNVPAESLIQE
                     QKILVEVPGLEGIPFNPAVRKYEVRLEPPSLDDTTPDLTRVLEGIELQGGPPPTSIYA
                     PLDVLCYLPLVLRRSGWHATATVALAPVGYRLSSVDEGNTTGRLYGAAVDLGTTTVVV
                     YLWSLVDGRILATASNYNRQISCGEDILSRVNFSRKSGLSRLQQLAAESINEALTTAA
                     DAAGIDREEIFEVMIAGNTVMTHLFLGIDPAYMIAEPYIPVVRRMLSTGAGQSGIAAH
                     KNAEVYTFPAVSNFIGGDIIADILATGMAEREEPTLMVDIGTNFEAVLGNSDWMLSCA
                     GAAGPALEGGEVLFGMRANPGAIERARIDAETLAPTCSTINGARPRGICGSGLIDLLA
                     ELFRACVIDRTGRINLEIGHPRVRKGQYYPEFVVVWKEETSLGKDIVITENDIRNLIM
                     SKAAIHGACMTLLDAAGLMPGDIGRVCFAGAFGNYLNKENAITIGLIPEISLERVENI
                     GNGAITGANIALLDRKRRKELDAIARKITYIELNAEPSFMDRYTSSCFLPHTDFTLFP
                     HVQQTLEACRARQGVRSWQA"
     gene            complement(10246..11580)
                     /gene="acsC"
                     /locus_tag="PLV90_04160"
     CDS             complement(10246..11580)
                     /gene="acsC"
                     /locus_tag="PLV90_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HOF96339.1"
                     /translation="MAMKALDIYKHLPKTNCKKCGYPTCLAFAMKLAVGKADVEACPD
                     LEAEARTLLGGVTRPPVRLVTIGTGERSVAVGEEFVLFRHEKTFYHPPGIVVQVSDAW
                     PDDEVRSVVEMVEETVVHRVGQDLVLNGVAVKYESNLPERFAEVVTLAGGGNSLPLAL
                     IADDPGALEAALAVAGHYRPLIHAATEENHEALAALAKRYGCPLAVRAADPAELAALA
                     GKCADAGVPDIVLDPAPSSLAGFVAIATDIRKAAIGRTEPALGYPLSIDAGAFGDADA
                     AIAAGILKYAGIIVVPPLSPASVEAALTLRQNIYTDPQKPIQVTPGIYPINEPGRNAP
                     VLLTVNFSLTYFTLLGYLEASRIPCYLFIADTEGLSVLTAVAGGKLTETLVADSIKEF
                     GVGDLVDHRLLVIPGYAAPLSGKIEDATGWKVLVGPRDAADLPGYLETEWKT"
     gene            complement(11584..12492)
                     /locus_tag="PLV90_04165"
     CDS             complement(11584..12492)
                     /locus_tag="PLV90_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HOF96340.1"
                     /translation="MESNDERLAIREVRLGATRAEGGTREVSYAIGGERDLPFSGKRP
                     ERLLVALEICDDPRFWPPVVRDYVGDLANDTAEWARAAERTYGADLVRLNFTSTKQRG
                     FDAFGAIAATTEEVLAATARPLIVEGSNEPELDSEVFRRSGEAGEGERLLLGTAEADR
                     YRSVAAAALAYGHAVIAQSPIDVNLAKQLNILLRETGVPQDRIVIDPYTGALGYGFEY
                     TYSVMERIRLAALAGDADLAMPMISAPTDTLSIREVREAAPADREAMAVAWEFYTAYA
                     AVVAGASIVCVRHPLTVARLKTALEG"
     gene            complement(12508..14544)
                     /gene="acsB"
                     /locus_tag="PLV90_04170"
     CDS             complement(12508..14544)
                     /gene="acsB"
                     /locus_tag="PLV90_04170"
                     /EC_number="2.3.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
                     /protein_id="HOF96341.1"
                     /translation="MKDRLTDSLVTAIEHGRTGLDARMQALAGKLAYPETAYALPVAY
                     AVTGIAVRDANGAREAYRQSGNNLLVACECLMAGEGGVASPYTGFIADAVLRRLGYTL
                     VDGSVTGLGLLVGTPESPDAAAAICREMQEKYLLTFLAGGVVEALSGAGVRLGLDYRL
                     VPLGPESAHGIHFADILARVAMMFGGVQPGDVHSLLGYAAERAKAFVIVFGGLTDEET
                     AFVDALRVLGIPILSAGGGYEGGEWIGIAPAEAVRTGMDLRGIRVTVTAIPIPMACSP
                     AFEGKSIRKEEMYVEFGGGRTPAFELLRMRPREEIDDGKVTVVGPDLDDVRTGSALPL
                     AILVDVAGARMKADYEPVIERRIHTFINYGEGSWHVAQRDLIWVRLSKEAVAKGVRIR
                     DIGTLLAHKLKAEFPDHIDAVQVTLVTDREQVERMLVEAREIYARRDERISGMKDEDV
                     ETFYSCTLCQTFAPNHVCVITPERPALCGAITWLDARVAHDIAPAGANQPVPKGEMVD
                     ALYGEFVGVNRFVKKASHGEVDRVSLYSMIENPMTACGCFECIAAIVPEVNGILVVSR
                     DFAGETPLGMTFSTLAGTIGGGAQTPGFIGISKNYILSDRFLAAENGIMRVVWMPSSL
                     KEELGDRLRERLAAQGVPDLFAKIADEVSAPTIEDLVLFLERVKHPALTMRSLI"
     gene            complement(14881..15942)
                     /gene="corA"
                     /locus_tag="PLV90_04175"
     CDS             complement(14881..15942)
                     /gene="corA"
                     /locus_tag="PLV90_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOF96342.1"
                     /translation="MSRHDERAEQNRMRGGQGMLISTGDTPAHSVTITVIDYDGSRVD
                     ERICRPEDLRALIARPTVTWIDVDGVHDAGIIEAVGDVAGIHPLTLEDIANTRQRPKI
                     EDYGNYLYVAVRMLSPDGAGAFQSKQMSLVLGTGYVISFQERPGDVFSRIREHLRTGA
                     GRIRNEGADYLFYSLLDAIVDGYFTVIEEFGERIEAVEEEVVADPDHETLQAIYALKR
                     SLIALRRSVWPLRDVVAELERGDSSLIRTSTLVYLRDVYDHAIQVAETVETYRDTASG
                     TLDVYLSSQSSRMNEIMKVLTIIATIFIPLTFIAGVYGMNFAYMPELGYSWGYPVTLA
                     LMAVVAGIMLFYFKRKGWF"
     gene            complement(15987..16238)
                     /locus_tag="PLV90_04180"
     CDS             complement(15987..16238)
                     /locus_tag="PLV90_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5654 family protein"
                     /protein_id="HOF96343.1"
                     /translation="MSFKTEVIEKIAALVTAAFGLVAALAWNGAIQELFKLIFGEQST
                     LVAMFVYAVVVTIIAVIAVVLIGRAAAKAKGEDEAAERK"
BASE COUNT         2985 a         5380 c         5126 g         2916 t
ORIGIN      
        1 acacattcaa ccggacattc accctcccgg catacaccga tatccagtgg gcacggctgt
       61 acacggtcgt ctacgccgca ggtacggaca accgggcggg gaccgtgacc gtcaggttcg
      121 acggcaacgg cacatacgcc acgctcggca tcgagaccct tgccaccgcc ggggataata
      181 cggcaaacgt ctacccggtc aacgaccacg tgaaccgcca gtattcggat tacctgctct
      241 ggtacgacgt gaccgatctc atcggttcag aggacccgaa agcggaggtc gtcgcaaccc
      301 cggtggcacc gaacttcgac ggccgcatca aggagctcgt cctcgtggtc gcgtacaacg
      361 acggcgacgg cgatcaggtg cactactggg tgaacgaggg gcacgactgg caggcaaacg
      421 gcgccgacgg ggtgacctcg acctttgcaa ccggcgggct tggtgccggc tggacggatg
      481 caaccctccg aaacgtcatg ctctcctcaa aagacgccct gtatacgttc aacggcgctc
      541 cctgtacggg tgcggactca tcgatcacgt tcagggcgaa cacctgggat gtcaggacca
      601 acctgaccgc cggcagcgag agccgtctca cgtacgtgcc gaacggcgga tcgtacaaga
      661 ccacgctcgc aacgcttgcc gtgagatacc cggcctccgt cgccctcccc gatctcacgg
      721 tctcgaccct cgcgccgaac agcggcgagg tcttctccgc atccgagaac acctacacgg
      781 cgacggtcac gaacatcggc gccgccgacg ccggcgcgtt tgcggtcggg ttcaacgcga
      841 gcggcgcggc cggcacggcc cccgtcgccg ggcttgccgc cggtgcgacc gccaccgtca
      901 cctggaccga cgagaccatc cgcaacgccg gcgacgtcgt gacgatcacg gcgaccgctg
      961 atgccgcaaa cagcgtcgcc gaggccgacg aggagaacaa ccataagact cttgtaaaga
     1021 cggtcgtcca caacggctac cgcggcaagc gctggaccgg cggagaggac atcgcgaccg
     1081 tgaagacgta caccgtccgc ggcgatctcg tctactcttc cggcaacagc acctacctct
     1141 ccgccagcac ctacccgaac tgggccggct acgccgtctc ctggacggcc ggcgatcttg
     1201 tggtcccggc gaacgccacc gtggcggctg cccggctcta cgtcccctac acctgggaca
     1261 agggcccggt cttcccctca aacgtcaccc tggccttcaa cgggaagacc gttgaccggg
     1321 ctgccttcta cgcggacgag aagatgtggg ggtcgtccta cccctacggc atgaccgcct
     1381 acgacgtcac cgatgccttc agcgtcgccg gcaacaccgc cgtcctcacg agcaccttcc
     1441 ccggcggcgg caacgtctcg gttcgcggga tgctccttgc ggtcgtctac gacgacggca
     1501 ggaccgcccc gcacacggtg ctggtgaacg agggcttcga cctcctctac ggcggcgcgg
     1561 cccaggggac aacccccgag caggcgacgg cgtatgcccc cttcgccatc gatgcgaagg
     1621 acgccgtcaa cgcccgcctg gtcaccgtcg cccccggtgc cgggccgaca aagggcgacc
     1681 tcctcttcaa tgaccgggtc tggacccgcg tctggaacta caccggcacg acccagatcg
     1741 gggttgacga ccgcgacgtg acggcgctcc ttgccggcga gaacctggcc gggttccgga
     1801 gcgacgccga ctacatggag gctgcggcag ccttcctggt cgtcgagtac ccggtcccgg
     1861 ccggggatac cgcgatcatc ctctccccgg gctggaactt cgtttcgacg cccaagaggc
     1921 ttgctgacgg tgcgaacacc ttcgccatat tcgatacggt cgataccgcc gcccactcga
     1981 tccagctcta cgacggtctg gagcggaagt ggaggcagat ggctccgacc gacccgttcc
     2041 ggccgctcga tgcggtctgg gtctacgcaa acaagacctg caccgttccc ctgaccttcg
     2101 ccgccggcac accagagctt ccgcccgcaa aagacctcgg gaaaggctgg aacgcgatcg
     2161 ggttcaccga taccgtccct gagacggcga actacaccct gcagtctctc ggcgatcact
     2221 ggtccatcct gatcgggttt gatgcaggca tccaggagta cgatacatcg atcgtccgcg
     2281 gcgccaccgg tccccatggg gatgagcgcc tgatgcgacc catgcagggc tactggctgt
     2341 acgtgaacgc ggcagatacg ctcggcagtc tcagcgggtg agaggaaagg cgtgcgacag
     2401 ggcatcaccc tcctcctcgc cctcgtcgcc ggcgctacgg cggccgggga ggtggcacct
     2461 cctgccttcc cccacgagtt ctacgggagc atgacgatcg acggtgcaac agccccgcct
     2521 cccctcaaaa gccatgatga ggaccgggat cgcaggtagc agccggcgag cgcctccacg
     2581 atcccgccgg caggaggccc gggcagtcgt ttctcctgca ctcccggtgg ctggccgcgg
     2641 cagcatcggg attatccggc gccccgatac ggaagtcgat gcaggtgacg cttccgtcga
     2701 tcgggcttcc cgagggccat gcccgggacg aaaccggtgc tccggtgccg gcagggtggg
     2761 gattccttcg gggcccgaaa ggtaaaatta ccgatccggc caaattacca gtacagatcc
     2821 cgaccgtggg tacccaggga aacccggcgg ggttcggggg acagcaccca tgagcgatta
     2881 ccaggacaaa ttcagcaaga tcgtcgatct tcttgaggag cggtcgcctc aaggactctc
     2941 tatctctgcg atcgcccgcg aactcgggat gaaccgtgct tccgtctcca agtacctcga
     3001 gatgcttcaa tcaagcgggg atgtgagcat gcagcgtttc ggcagatcaa aattatatac
     3061 accggcgcat caggttccct tctcggagct ctttgaccgt ctctccagcg cgatcgtcat
     3121 cctggatgcg gatcttcgca ttctcatggt aaacacaagt ttcatcaagg ccctcggcat
     3181 ccaccgcgag cgaaacatca tcggtgcgcc gctctttgac ttaaacctcc gcatcctctc
     3241 agatccggcg atcaggcgaa atatcgagag gatccgggct gccggaccgt atcttgccga
     3301 gatgcagcac attgaggata gcacgaacca tatccatctc atcgagttcg ccccgatcgc
     3361 atcccgcgca ggaaagcccg ggatcatgat cagcctccgg gatatcacgg catggaagaa
     3421 tgccgaggtg gccctgaaga actcagaaaa gaagatccgg acgatcttcg agacggcccc
     3481 gagcgggatc gtcttttttg cggtcgacgg caccattctc aacgcaaacc gcgcgtccct
     3541 gcggatcctc ggtctgaagg attttcagga gctggtgaac gggaacatct tcgatatctc
     3601 ctgctataaa gggaagattc ttgaactgat ccggcaggga aaggtcgccg agacggaact
     3661 cgtctgtgat ttcgacctcc tgaagcagga acagcgaatc ccgagctccc gatcgggcat
     3721 cgcctacttt gatgttgtct tcacccctat cgccctcgat ggcgggggag ccccgcgcga
     3781 gtttgccata ctcttctggg atgtcactac ggagcggttg acccggaaag agctggtgtt
     3841 caaagaaacc cgctaccgat cgttcttcga gaatacctgc aacggtgtcc tgatctacga
     3901 accggtcgac gatggcaacg agtatatctt caaggatatc aaccatgcta cagagcagat
     3961 cctccggatg aagaaggaag atctgatcgg gagaaaactc ttcgaggtct tccccgatct
     4021 ccccgacccc gatgttcgga acgctcttat ccgggtcttg aaaaccgaaa agcctgagtt
     4081 cctgctgccc ctccagtatc ggaaagggaa ggattctccc tggatctggc actatatatt
     4141 caaactaccc tccggggaga tcgcatcgtt catgatcgat gtctccgatg ctattcgcga
     4201 ggatgctgaa gggccgctcc gggcatgcgg tatgcatagg cagcattccc gggatcaacc
     4261 ggctccggtg tcccacgccg attgattcat gggggcgcct ggacagcgta tccggaggcg
     4321 ggacccatgc ccgaacgccg atgctggaaa ggcggtcgtg tgtgaaacgc tgtccgggaa
     4381 gcggcagcat atggggagga gaatgccgat cccccctcct gttttcttcc ggggccttct
     4441 cgctcctgcc ctattcgcgc gaacaaataa cccaatggag ttaacttatg gtttcaatat
     4501 aaaaatatat tgtttattga ttaacgttgc accgcgccgg cgccagccgg cctatccgca
     4561 cgccgcagta cactcgatgc cagaagaggt gttatatttc acatttattc aataattgag
     4621 aaaatcactg caattttaat ccaggggcgg gtgccgcacc gcctgatatc ccgtataatt
     4681 gtcggagtta ttgttaatca ataaacaaca cctatatcta cagttagata tattacttct
     4741 tatgtggacg accagtccgc atacgacaga atcggaagag aaccattaac ggggtaggat
     4801 ggccttggtc cgctacaggc aagtgggatt gacgtgcggg cctcagctgc cgtaccagtg
     4861 ttttcattgg cgctggtaac atatcaagat tagggtagtc ttgcctccgg atagggtact
     4921 ctgccgatcg acaggagacc agtaccatga agacacagat cggttacttt gcatctctgg
     4981 agcagtatcg ccccatggat gcgcttgaac aaacaatcag agcagaaaag gtcggcttcg
     5041 actcgatatg ggcggacgac cacttccatc cctggtacca cgacaatgcg cagtcggcgc
     5101 aggcctgggc ctggatgggt gcagcgctcc aggcaaccaa gagagtattc atctcgacct
     5161 gtatcacctg cccgatcata cgctacaacc cggcaatcgt cgcgcagacg tttgccaccc
     5221 ttcggcagat gtacccgaag agagtcggcg ttgccgtggg tgccggcgag gcgatgaacg
     5281 aagtgccggt gaccggcgta tggccgagcg ttgccgaacg gcaggacatg accgttgaag
     5341 ctatcgaggt gatgcggaag ctctgggaga gcaaggagcc cgtcaccttc aagggcaagt
     5401 tcttcactct tgacaaggcg ttcatgtaca ccaaacccga cgatgaggtc ccgctctact
     5461 tcagcggcct cggccccaag ggtgcgaaac tcgccggcat gtacggcgac cacctcatga
     5521 ccgttgcagc cgccccgtcg gttctcaaga acgtcaccat ccccaagttc gaggaaggcg
     5581 cccgcgaggc cgggaaggac ccgagcaaga tggagcgcgc gatgctcatc tggtactcgg
     5641 tcgaccccga ctacgagaag gcggtcgagg gcaaccggtt ctgggcggga tgccttgtcc
     5701 cctcgatgtt caagtacaag gtctacgacc cgaaggaagt ccagctccac gcaaacctcg
     5761 tccactgtga caccatcaag gagaactaca tgtgtgccac ggatgccgaa gagatgatca
     5821 aggagatcga gcggttcaag gcggcaggca tcaaccactt ctgcctcggc aactcgagcc
     5881 cggatgtgaa caccggcatc gatatcttca aggagatcat ccctgccgtc agggattgaa
     5941 ccgggcatca ccggtccatc cccatttccc tgaggaacga tggaaaacca gagcattgcc
     6001 cggctcatgg aggagggtgc cgccttcttc tccgccgggg aagcgggcag acggttgacg
     6061 tggtatacga atccggcgtg gaccggcgtc tccctggcgg atcttgcgcc gggagcggat
     6121 acaaaggggt cgttcacctc ccacttcgtc aggattagga aaggctgcga ggttcccgac
     6181 catctccacg agagccagtg ggagtggaac gtcatcctgg acggccacgc gaagatggtc
     6241 ctcgacggga gggagatccc ctttaagccc ggagacacct tcaccacgcc gcccggtgtc
     6301 cgtcacgccg tcgttgccgg caaggaggat gtcgcgctgc ttgcggtatt tgttccgggt
     6361 ccggcatgac ggcaggggtc tttcgcctca ttctccccga taccacacct ccgcgtccgg
     6421 tgcatccggg tgcggggatc ccctccccac tccctatatc tccccgctga aagatcggca
     6481 ccttccccat atgggccgat ggaaccccgg ggcaccttca atatccgcct catccttttt
     6541 tatattggag catcacccgc gccgggcgca ctcctcgatg agccatccgg cgagcatcct
     6601 caccgccgcc cgtgccggac tctcctgcgg tataccgacg atcggcgtcc ccgcaagatc
     6661 cgcctcccct acgccccggt cctccggtat ccgggcgacg aggggaagat tgctctctac
     6721 gacggcatcg ccccggtcac ggttgacggc gaggaatacc cggtcgcgcg agaggccgag
     6781 cccctccgcg aggtcgcgga tgcggccggc ggtccgtata ccgcgggcag agggatcgct
     6841 cacgatcagg aggacgtccg ggttcttgat cgttccccgg gcgatatgct ccataccggc
     6901 ctcggagtcc acgacgacga accggtagcc gcgttcgagc gtcccgatgc actcggtgag
     6961 gagggcgttc gggaagcagt agcaccccga cccctccggc cggcccatgg cgaggaggtc
     7021 gtagccctcc gcctccgcga gagcccggcg gaaccggtat ttgacgtagc ccgtcctgtc
     7081 catgcccggg ggtatggccc gggtgaaggc ttcctcccgc atgcccccga gcgtctccgt
     7141 gagcgcgatc ccgagcgcct cgtggaggtt tgcgttcgga tcggcgtcca cggcaaggat
     7201 tggccgctcg ccggccgcga taagcgcatc gatcaggagc gccgcgatcg tcgtcttccc
     7261 ggtaccgcct ttgccggata tgacgatggt gcagggccgg gtcatggctc ctcaccccgg
     7321 aggcggcggg agagcgacgc ggttgctgcc atgatctcga tggcctcctc ctcgttagcg
     7381 ttccggatcc cgcaggtcgg cgtgatcagg gattggcggt cgaagagatc ggggtcgatc
     7441 gtctccgtcg cccgggcgcg gatctcttcg aagcgggaga agaacgcgtc gggcttctcc
     7501 gccgcaaagg cggcgtggtc cgcggggacg ataccccagg ccaccacgcc tcccctctcc
     7561 atgtagcggg cgatctcgtc ggcatacagc agaaactcgc gggcgtaggt ccaggcgtcg
     7621 acggagacca cggccggcgc aagaccgagg acgaaccccc agtcggtgtt ggcgcagcag
     7681 tggatcccga gccccccgtc gaggagggcg gcgatatcct cgaaggctga cgccgccacc
     7741 ccggcatcca ccgggacgac cgccgaaccg agcgacgcga ggtagggatc gctcagaacc
     7801 accagggtcg cgcgtgcgcc catccgctcc cgcatcgcgt tctcgcacca ccgggcccgc
     7861 aggccgagga gttttccgag caggtcggca tactccgggt cgtagaggat gggccggcgc
     7921 gcctcgtcca ccacctgcat gccgaaggtc accgggccgg tcacctggca cttgacgagc
     7981 agtgcgtccg agaggtcgcg gcccatcatg gcgtggaacc ccgacccgta agccttgccg
     8041 acccggtagg gctccgcgtt ccgttcaagg tagtcgagca ggacctgctc catcgcctca
     8101 gagggatcgg tgctccggtc gatcacgagc ctgccgtccc ggaccacccc gccggggagg
     8161 tgttcggcat cggcaaagac gatcgcctcg agaaaccccc ggttcggcag ggtgggtaca
     8221 taagggatcc gggggaaggc cgcgagaacc gcccggcacg cctcaatcgg gtcgcggtgg
     8281 gggagcgccc ccacgccggt cgcgaggagc tccggcggcg gtatcatgcc tgccacgacc
     8341 tcaccccctg tctcgcccgg cacgcctcaa gtgtctgctg cacgtgcggg aagagcgtaa
     8401 agtcggtgtg cggcaaaaag cagctcgatg tgtaccggtc catgaaggac ggctcggcgt
     8461 tcagctcgat gtaggtgatc ttccgggcaa tcgcgtcgag ctcctttcgc cgcttgcggt
     8521 cgagcagggc gatgttcgca ccggtgatcg ccccgttccc gatgttctcc acacgctcca
     8581 gggatatctc gggtatcaac ccgatggtga tggcgttctc cttattgagg tagttcccga
     8641 acgcccccgc aaaacagacc ctgccgatat ccccgggcat aaggcccgcc gcgtcgagga
     8701 gcgtcatgca ggccccgtgg atcgctgctt tgctcatgat caggttcctg atgtcgtttt
     8761 cggtgatgac gatatccttc ccgagcgacg tctcctcttt ccagaccacc acgaactccg
     8821 ggtagtactg ccctttccgg acccgcggat ggccgatctc caggttgatc cgtcccgtcc
     8881 ggtcgatgac gcaggcccgg aagagctccg cgagcaggtc gatgaggccc gagccgcata
     8941 tcccccgcgg tcgtgcaccg ttgatcgtcg agcaggtcgg tgcgagcgtc tcggcgtcga
     9001 tccgggcccg ctcgatcgca ccggggtttg cccgcatgcc aaagagcacc tccccgccct
     9061 ccagggccgg ccctgccgcg cccgcacagg agagcatcca gtcgctgttg ccgagcaccg
     9121 cctcgaagtt cgtgccgatg tccaccatga gcgtgggttc ttcgcgctcc gccatcccgg
     9181 ttgccaggat atcggcgatg atatcgccgc cgatgaagtt cgagaccgcc gggaaggtgt
     9241 agacctcggc gttcttgtgg gccgcgatcc cgctctgccc ggcacccgtc gagagcatcc
     9301 gccgcacgac cggtatgtaa ggctcggcga tcatgtaggc agggtcgatc ccgaggaaga
     9361 ggtgggtcat caccgtgttc ccggcgatca tcacctcaaa gatctcctcg cggtcgatcc
     9421 ccgcggcatc ggcggcggtc gtcagggcct cgttgatgct ctccgccgca agctgctgca
     9481 gccgggaaag gccgcttttg cgggagaagt tcacccgcga gaggatgtcc tcgccgcagg
     9541 atatctggcg gttgtagttc gatgccgtag cgaggatccg cccgtcgacg agactccaga
     9601 ggtagaccac gaccgtggtg gtcccgaggt ccaccgccgc cccgtagagc ctccccgtgg
     9661 tgttgccttc gtcgacgctg ctcaagcggt agccgacggg cgccagggcg accgtggcgg
     9721 tcgcgtgcca cccgctccgg cggagcacca gcgggagata acagaggacg tcgagcggtg
     9781 cgtagatact ggtcgggggc gggccgccct gcagctcgat cccttccagc acacgggtga
     9841 gatcgggggt cgtgtcgtcc agggacggcg gctcgaggcg gacctcgtac ttccggacgg
     9901 cagggttgaa cgggatccct tccaggccgg ggacttccac caggatcttc tgctcctgga
     9961 tgagcgactc ggccgggaca ttcacctgga tgtcgccgac gacggtcgcc tggcaggcaa
    10021 gacacgcccc gcgggcgatc tcgtcgtccg agaagaacct gcggtatttc tcgcggtcga
    10081 acgttaccgt gccctctttg atgaagacaa ggcacttccc gcacttcccc tgcccgccgc
    10141 agaccacgtt catggagagg ccggccgcgc gcgccgcgtc gaggagagtc gttccctccg
    10201 gcacggtcac cttccggtag cccggaagga acgtcacggt cggcatcacg tcttccactc
    10261 cgtttccagg tatccgggga gatcggccgc atcacgaggg ccgaccagca ctttccagcc
    10321 ggtcgcgtcc tcgatctttc cggagagggg ggcggcgtag ccgggaatga cgagcagccg
    10381 gtggtctacc agatccccga cgccgaactc cttgatcgag tcggcgacga gggtctccgt
    10441 caatttcccg ccggcgacgg ccgtgaggac cgagagcccc tcggtatccg cgatgaagag
    10501 gtagcagggg attcgagacg cctcgagata cccaagcaac gtgaagtagg taagcgagaa
    10561 gttcaccgtg aggaggaccg gggcgttacg cccgggctcg ttgatcgggt agatgcccgg
    10621 cgtcacctga atgggtttct gcgggtcggt gtatatgttc tgccgcaggg tgagcgccgc
    10681 ctccacggag gcgggggaga gcggggggac gacgatgatc cccgcgtact tcagtatgcc
    10741 cgccgcgatg gccgcatcgg catccccgaa ggcgccggcg tcgatggaga gggggtagcc
    10801 gagcgcgggt tcggtccgcc cgatggcagc cttccggata tccgtcgcaa tggcgacgaa
    10861 gccggcaagc gacgacggcg cagggtcgag cacgatatcg gggacaccgg cgtctgcaca
    10921 cttcccggcg agggcggcga gttcggcagg atcggcggcc cggacggcaa gggggcaccc
    10981 gtaacgcttc gcgagggcgg caagggcttc atggttctcc tccgtcgcgg cgtggatgag
    11041 cggccgatag tgccccgcga ccgcgagggc cgcctcaagg gccccggggt cgtcggcgat
    11101 cagcgcgaga gggaggctgt ttccgccccc ggcgagcgtc acgacctctg caaaccgttc
    11161 ggggaggttg ctctcgtact tcaccgcgac cccgttcagg acgagatcct ggcccacccg
    11221 gtggacgacc gtctcttcga ccatctccac gacggagcgc acctcgtcgt ccggccaggc
    11281 atcggagacc tgcaccacga tccccggcgg gtggtagaag gtcttctcgt gccggaagag
    11341 gacgaactct tcgccgaccg caaccgaccg ctcgcctgtg ccgatggtga cgagccggac
    11401 cggcggccgg gtcaccccgc cgaggagggt ccttgcttcg gcctcgaggt cggggcaggc
    11461 ctcgacatcc gccttgccga cggcgagctt catcgcaaag gcaaggcacg tggggtagcc
    11521 gcacttcttg cagttcgtct tcgggaggtg cttgtatata tcgagcgctt tcatcgccat
    11581 gtttcagccc tccagggccg ttttgagcct tgccacggtc agggggtggc ggacgcagac
    11641 gatcgacgca ccggcgacga cggcggcgta ggcggtgtag aactcccagg caaccgccat
    11701 cgcctcccga tccgccggtg cagcctcccg cacttcccgg atcgagaggg tgtcggtggg
    11761 ggcgctgatc atcggcatcg ccaggtccgc atcgccggcg agggcggcga gccggatccg
    11821 ctccatcacc gagtaggtgt actcgaaccc gtagccgagc gctcccgtgt aggggtcgat
    11881 cacgatccgg tcctgcggca cgccggtctc gcggaggagg atgttcagct gcttggccag
    11941 gttgacgtcg atgggcgact gggcgatcac ggcatgaccg taggcgagcg ccgccgcggc
    12001 gacgctccgg tagcggtcgg cctccgcggt cccgaggagg aggcgttcgc cctctcccgc
    12061 ctccccagag cggcggaaga cctcgctgtc gagttccggt tcgttgctcc cctcgacgat
    12121 caggggccgg gccgtcgccg cgagcacctc ctccgtcgtc gcggcgatcg cgccgaacgc
    12181 atcaaacccg cgctgtttcg tgctcgtgaa gttcagccgc accaggtccg caccgtaggt
    12241 gcgctccgcg gcccgtgccc actcagccgt gtcgttcgca aggtcgccca cgtagtcgcg
    12301 gacgaccggc ggccagaacc gcggatcgtc gcagatctca agcgccacca gcagtcgctc
    12361 cggtctcttc ccggagaacg ggagatcccg ttcgcccccg atcgcgtagg agacctcccg
    12421 ggttccccct tcggcccgcg tggcgccgag gcgcacctcc cggatcgcca atcgctcatc
    12481 attgctctcc atcacgcacc atccctccta tataagcgac ctcatcgtga gcgcggggtg
    12541 tttgacgcgc tcgaggaaga ggacgagatc ctcgatcgtc ggcgccgaga cctcatcggc
    12601 gatctttgca aagaggtccg gtactccctg tgcggcaagt ctctcccgca gccggtcgcc
    12661 gagttcctct ttcagggaag acggcatcca gaccactcgc atgatcccgt tctcggccgc
    12721 cagaaacctg tcgctcagga tgtagttctt cgatatgccg atgaaccccg gcgtctgcgc
    12781 gccgcccccg atcgtccccg caagcgtcga gaaggtcatc ccgagcggcg tctccccggc
    12841 aaagtcgcgg ctgacgacga ggataccgtt cacctccggc acgatggccg cgatgcactc
    12901 gaagcacccg caggcggtca tcgggttctc gatcatgctg tagagggaga cccggtcgac
    12961 ctcaccgtgg gatgccttct tgacgaaccg gttcaccccc acgaactcac cgtagagggc
    13021 gtcgaccatc tcccccttcg ggaccggctg gtttgccccg gcgggggcta tatcgtgggc
    13081 aacccgggcg tcgagccagg tgatcgcccc gcagagcgcc ggccgctccg gcgtgatgac
    13141 gcagacgtgg ttcggggcaa acgtctggca gagggtgcag gagtagaacg tctccacatc
    13201 ctcgtccttc atgccgctga tccgctcgtc gcgccgcgcg tagatctccc gcgcctccac
    13261 aagcatcctc tcgacctgct ccctatcggt gacgagcgtc acctggaccg cgtcgatgtg
    13321 atccgggaac tccgccttga gtttgtgggc aagcagcgtc ccgatatccc ggatcctgac
    13381 gccttttgcc accgcttcct ttgagagcct gacccagatg aggtcccgct gcgcaacgtg
    13441 ccaggacccc tcgccgtagt tgatgaaggt atggatccgc cgctcgatca ccggttcgta
    13501 gtccgccttc atcctcgcgc cggcgacgtc caccaggatc gcgagcggaa gggcggagcc
    13561 tgtccggacg tcgtcgagat ccgggccgac gacggtgact tttccgtcat cgatctcctc
    13621 gcggggccgc atccggagga gctcgaacgc gggagtccgt ccgccgccaa actccacgta
    13681 catctcctcc ttgcggatgc tcttcccctc gaatgccgga gaacaggcca tgggtatcgg
    13741 tatcgccgtg accgtgaccc ggatcccccg gaggtccatg ccggtccgga ccgcctccgc
    13801 gggagcgatc cctatccact cgcctccctc gtagccgccg ccggccgaga ggatcggtat
    13861 gccgagcacc cgcagggcat cgacgaacgc ggtctcctcg tcggtgaggc cgccaaacac
    13921 gatcacgaac gctttcgccc gctctgcggc atacccgagc agggagtgga cgtctcccgg
    13981 ctgcaccccc ccaaacatca tcgcgacccg ggcgaggatg tcggcgaagt ggatgccgtg
    14041 agcgctctct ggaccgagcg gcaccaggcg gtaatccagc cccaggcgga caccggcgcc
    14101 ggagagcgcc tccacgacac cgccggcgag gaaggtgagc aggtacttct cctgcatctc
    14161 ccggcatatg gccgcggcgg catcagggct ctccggcgtc ccgacgagga gaccaaggcc
    14221 cgtgacgctc ccgtcgacca gggtgtagcc gagcctccgg aggaccgcat ccgctataaa
    14281 gccggtatac gggcttgcca cgccgccttc gccggccatc aggcattcgc aggcgacgag
    14341 gagattgttg ccggactgcc ggtatgcctc gcgcgctccg tttgcgtccc gcacggctat
    14401 gccggtgacg gcgtaggcca cggggagggc gtatgccgtc tccgggtagg cgagtttccc
    14461 ggcgagtgcc tgcatcctcg cgtcgaggcc ggtgcgaccg tgttctatcg ccgtaacaag
    14521 agaatccgtg agtctgtcct tcatgatgcg ttcaacagat ggtactcttc gcacggcata
    14581 aaacattgtc tccgtgcagg acggcgccaa tcgcggtctt tggggctctc cggccggtga
    14641 tgcctatccg ggatcacggg cggatctctt cggcacacgc cgccaggaag gggtctgtac
    14701 ctccgtggaa aggccggtgc ctccgtgcag gggggcacgc tcggtgagat ccatggctgc
    14761 ccatctcccg gatatctcac cgggataaaa catcactgcc ggtggggccg gactccggcg
    14821 taatgggggg gatatcccgg ttacgggtgg gcgatggcga tcccgcccgc ccttcgccgg
    14881 tcagaaccag ccctttctct tgaagtagaa gagcatgatg cccgcgacga ccgccatcag
    14941 ggcgagggtc acggggtatc cccaggaata cccgagttcg ggcatatacg caaagttcat
    15001 gccgtagacc ccggcgatga aggtgagcgg tatgaatatg gtggcgatga tcgtgagaac
    15061 cttcatgatc tcgttcatcc ggctgctctg gcttgagagg tagacgtcga gggtcccgga
    15121 tgccgtgtcc cggtaggtct cgacggtctc ggcaacctgg atggcgtggt cgtagacgtc
    15181 gcggaggtag acgagcgtcg atgtgcggat cagggacgaa tcgccccgct cgagttccgc
    15241 caccacgtcg cggagcggcc agaccgaccg ccggagagcg atcagcgacc gtttcagggc
    15301 gtagatcgcc tgcagggtct cgtggtcggg atccgccacc acctcctcct cgaccgcctc
    15361 gatccgctcc ccgaactcct cgatcacggt aaaataccca tcgacgatcg cgtccagcag
    15421 cgagtagaag aggtagtccg ctccttcgtt ccggatccgc ccggccccgg tgcgcaggtg
    15481 ttcgcggatg cgggagaaga cgtcgccggg ccgttcctgg aacgatatga catatcccgt
    15541 gccgaggaca aggctcatct gcttgctctg gaacgcaccc gcgccgtcgg gggagagcat
    15601 ccggactgcc acgtagagat aattgccgta atcctcgatc ttcgggcgct ggcgggtgtt
    15661 ggcgatatcc tcgagcgtca gcggatggat tccggcgacg tctccgacag cctcgatgat
    15721 cccggcgtcg tggacaccgt cgacgtcgat ccaggtgacc gtcgggcggg cgatgagtgc
    15781 ccggaggtct tccggccggc agattcgctc atcgacccgt gacccgtcgt agtcgatgac
    15841 cgtgatggtg accgaatggg cgggtgtatc cccggtggat atgagcatcc cctgcccgcc
    15901 tctcatccgg ttctgctctg cgcgttcatc gtgccggctc atcggacctg cgaactccgg
    15961 gtcaaaaaga gaagggttgc cggggcttat ttcctctctg ccgcctcgtc ctcgcccttc
    16021 gccttcgctg cggcgcggcc gatcaggacg accgcgatca ccgcgatgat cgtcacgaca
    16081 acggcataca caaacatcgc aacgagcgtg ctctgttcac cgaatatgag tttgaagagc
    16141 tcctgaatgg cgccgttcca cgcgagggcg gcgaccaggc cgaatgcggc cgtaacgagg
    16201 gctgctatct tctcgatgac ctcagtcttg aaggacatgt ggtttcctca accgggaagt
    16261 gtacggtaaa cgatataata ctttcgcatt tcggcttttt ccggggtttt tcaggcaata
    16321 gggcctctat tgggagtcca aagggcaaac gagcggacat atgcgcgatg ggaagcgttc
    16381 ccggcgggat cgcggggatc aggtccc
//
LOCUS       DAOSII010000035        16382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_3715_length_16382_cov_50.615300, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000035 DAOSII010000000
VERSION     DAOSII010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 16382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16382
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3715_length_16382_cov_50.615300"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /gene="rplF"
                     /locus_tag="PLV90_04185"
     CDS             complement(<1..278)
                     /gene="rplF"
                     /locus_tag="PLV90_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOF96344.1"
                     /translation="MGILRQIEIPPGVNARLEGSVLTVSGPKGTLVRDMRFPQIDVRI
                     ADGEVIVSTASDKRRFLAMSGTLEAHVKGMFRGVVEGYEYRMKVVYSH"
     gene            complement(293..685)
                     /locus_tag="PLV90_04190"
     CDS             complement(293..685)
                     /locus_tag="PLV90_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOF96345.1"
                     /translation="MARLNPIADAMSTIKNAGDAGKSEVIVEPAGKLLGAMLRVMQEN
                     GFIGGFEFIDDGRGGQFRVQLPGTINKCGAITPRFSVGMADMEYWESQYLPAKNFGIL
                     IVSTSKGVISHEQARGEGIGGQLLGYVY"
     gene            complement(699..878)
                     /locus_tag="PLV90_04195"
     CDS             complement(699..878)
                     /locus_tag="PLV90_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOF96346.1"
                     /translation="MAGKSGASAPGAGVKRFGRGANECRICGRKQGLVRKYGIYFCRQ
                     CFREWAGRMGFKKMS"
     gene            complement(882..1385)
                     /locus_tag="PLV90_04200"
     CDS             complement(882..1385)
                     /locus_tag="PLV90_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOF96347.1"
                     /translation="MSAMKDIYIDKVVVHMGVGESGERLVKAEDLVKKITGQKPVRTI
                     AKRTQPAFGIRKGAPIGCKVTLRRANAEKFVETALAIVERHLAASQFDRTGNVSFGIE
                     EHTDFPGMAYDPAIGIFGMDVNVVLERKGVRIARRSVERRKLPAKQRVNQEEAIAFMR
                     ERYQVEV"
     gene            complement(1382..2110)
                     /locus_tag="PLV90_04205"
     CDS             complement(1382..2110)
                     /locus_tag="PLV90_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4e"
                     /protein_id="HOF96348.1"
                     /translation="MSNHLKRLVAPGSWHIPKKVQKFVMKTAPGPHNAGALPVGVWLR
                     EHIGLAGNASEVRKILHQRDVIVNGRACRNPQMGLGVFDIVSIPKLAKHYRIQLDKRG
                     NLIAVDISAASAKTRLCKIRNKTTVRGGRVQLNLAFGANVLADNTYRAKDSIVVTLGT
                     DGEERFRIVDHFPFEEGNVAMVVGGKHSGKVARIVEIARTASSVPNRVILVDDSTDER
                     FETIEEYIFMVGRTAIAPELEASA"
     gene            complement(2110..2475)
                     /gene="rplX"
                     /locus_tag="PLV90_04210"
     CDS             complement(2110..2475)
                     /gene="rplX"
                     /locus_tag="PLV90_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOF96349.1"
                     /translation="MVRIVSKQPRKQRKARYNAPNHTRGRFLSASLSPELRGKYSVRR
                     TRVVRGDTVKVLRGDFAKEEGIVDAVDMKMCRLIVHGVMVTKSDGTEVPRPVDPSNVQ
                     ITKLNLKDKLREERLGGGA"
     gene            complement(2489..2887)
                     /gene="rpl14p"
                     /locus_tag="PLV90_04215"
     CDS             complement(2489..2887)
                     /gene="rpl14p"
                     /locus_tag="PLV90_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOF96350.1"
                     /translation="MKAMQSTIPRALATGSRMVCSDNTGARLVEVVSVDGYHGVRRRQ
                     PCLGVGDVATVSVKKGTPDMRRKLEKAVVIRQKKEIRRPNGIRLSFEDNAMVLINERG
                     EPKGTEIKGPVPREIAERFPKIASMATIIV"
     gene            complement(2884..3210)
                     /locus_tag="PLV90_04220"
     CDS             complement(2884..3210)
                     /locus_tag="PLV90_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOF96351.1"
                     /translation="MARNIGLDVAVPETECDDANCPFHGTLPVRGQVITGKVVSDRMN
                     GTVVVEREFLHYVKKYKRYEKRRSRYHAHSTPCINAGVGDVVRIAECRPLSKTKNFVV
                     VEVMKE"
     gene            complement(3220..3492)
                     /locus_tag="PLV90_04225"
     CDS             complement(3220..3492)
                     /locus_tag="PLV90_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component 1"
                     /protein_id="HOF96352.1"
                     /translation="MISPQNILRHELIGLDVLVARASNPGHVGVSGRIIDETKNTLVI
                     QTGRGEARIPKRFSVFRIRLPDGTTVDVDGSSLEMQPERRISMRIK"
     gene            complement(3489..3692)
                     /gene="rpmC"
                     /locus_tag="PLV90_04230"
     CDS             complement(3489..3692)
                     /gene="rpmC"
                     /locus_tag="PLV90_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOF96353.1"
                     /translation="MAIFRAHEVRQLTDTELLEQEQKLSLELIQERGKVSAGGATENP
                     GQIREIRRTIARIRTEQNARRTA"
     gene            complement(3693..4385)
                     /locus_tag="PLV90_04235"
     CDS             complement(3693..4385)
                     /locus_tag="PLV90_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOF96354.1"
                     /translation="MAIEKKFITDGVRNVRVEKFLTKELKRAGYGGMDITRTPLGTQV
                     TIFAEKPGIVIGKGGKQVRQLTQDLAAAYAIESPQVEVQQVQNPNFNAQIMAERLANA
                     LERGWYFRKAGQSTIRRVMDSGALGCEVIIAGKLTGARARTQKFTEGYVKHCGEPSET
                     IVEKGYAVAVKKLGTIGVQVKIVPPDARLPDTFDVLAPAPKQAPVPPAEPEEVGEDEF
                     EDIPDEEYVEER"
     gene            complement(4385..4846)
                     /locus_tag="PLV90_04240"
     CDS             complement(4385..4846)
                     /locus_tag="PLV90_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOF96355.1"
                     /translation="MARTEYSANIEGENVARAKANELPISPKHSIEIARFIKNMTTTE
                     ARAYLADVVELKKAIPFKRFNRNVAHKRGLDRWAAGRYPVKAAEAYIRLLKSVEQNAE
                     YIGLDTENLRIDHVAANTGRGLRAFFPRAMGRATPKRRETVNIEIVVTEVA"
     gene            complement(4852..5265)
                     /locus_tag="PLV90_04245"
     CDS             complement(4852..5265)
                     /locus_tag="PLV90_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOF96356.1"
                     /translation="MAKKTQKRMPRRREEFTYRGHSVTDLQQMALSELLPIMPARARR
                     KFDRGLSRDHEKLLADVRSGDETIRTHLRDMVIMPEMVGRTIQIHNGKEFQKVEIQPE
                     AVFHYLGEFALTRKRVSHGSAGIGATRSSKYVPLK"
     gene            complement(5276..5995)
                     /locus_tag="PLV90_04250"
     CDS             complement(5276..5995)
                     /locus_tag="PLV90_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOF96357.1"
                     /translation="MAHRIIAQNRGKGGPTYRAPSHRYKAELRHAGKNTTLTSGRVVD
                     IEHDPARHAPIALTRLDDGKKIYVLATEGLGVGDTVTWGSGGAVKNGNTLPLQEIPVG
                     AYICNIEARPNDGGKFVRASGVQALVIGKADDGRVGVRMPSGKNKWFSGVCMATVGIV
                     AGGGRGEKPFAKAGKKHFHVKSSAEKWPRVKGVCMNVIDHPFGGGGHQHCGRPKTVSR
                     GTSPGRKVGHIAARRTGKWKK"
     gene            complement(6008..6256)
                     /locus_tag="PLV90_04255"
     CDS             complement(6008..6256)
                     /locus_tag="PLV90_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOF96358.1"
                     /translation="MILKYPFVTEKATMMLEGENKLQFIVQKNATRQEIKRELEAAFE
                     QDVVAIRTMMTMKGEKKAIVTFSDAKAAEEILSRLGIM"
     gene            complement(6253..7002)
                     /gene="rpl4p"
                     /locus_tag="PLV90_04260"
     CDS             complement(6253..7002)
                     /gene="rpl4p"
                     /locus_tag="PLV90_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOF96359.1"
                     /translation="MKAQVRTLAGEIAHEIDLPEIFDEEYRPDLIKRAVLALQSTRFQ
                     PHGTDPYAGLRTSAESWGSGRGVAQVPRLKNGSRVARVPQATGGRAAHPPKVAKVLVK
                     EINRKEKQKALRSAIAASTSPELVRGRGHLFEGDLPLVFEDRFEEVTRTADVIAALSA
                     LGVYADVERAKGSKKVRAGRGTMRGRRYKQRKSILIVTGDEPLRAARNLAGVDAVAVD
                     QLNAELFAPGTEAGRLTVWTESAIRRLEELS"
     gene            complement(7009..8022)
                     /locus_tag="PLV90_04265"
     CDS             complement(7009..8022)
                     /locus_tag="PLV90_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOF96360.1"
                     /translation="MPKYNRPRRGSLAYSPRKRAKSPVPRYGSWPEYKGAPAVQGFAG
                     YKVGMTHVIMVDDHKSSPTEGRDVMVPVTVVEVPPMRVAGVRAYCEDTYGKHALTEAW
                     TTDLDDELSRRINVPKNHDTAAALDAIKAAIGEGRVTELYALAYTRPMELTGVPKKVP
                     DLMEMRIAGGSLDDQVTYAAGVLGKEVVISGNLKVGEYVDVTAVTTGKGTEGPVKRWG
                     VQVRKRKHSRGGKKRHIGNLGPWHPHHVRWQVPQMGQMGYQQRTEFNKRILKIGTNGD
                     DITPEGGFLHYGLVRGPYVLIKGSVPGPNKRLVRIRPAIRQGEHTVRTPTINFVSAQS
                     KQG"
     gene            complement(8820..9224)
                     /locus_tag="PLV90_04270"
     CDS             complement(8820..9224)
                     /locus_tag="PLV90_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfx family DNA-binding protein"
                     /protein_id="HOF96361.1"
                     /translation="MKQGLLTDRQKEVLRYRKRGLTQQQIADIIKTSKANVCTIEKAA
                     LENIARARETLEFLYTLDAAHLCTLEGGLDLLKAPEIIYAEAERMGVKVKYDTISLIN
                     RLRDANPDRFRGRQVRDDVEIYINQDGDLYFG"
     gene            9366..10208
                     /locus_tag="PLV90_04275"
     CDS             9366..10208
                     /locus_tag="PLV90_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-dependent methylenetetrahydromethanopterin
                     dehydrogenase"
                     /protein_id="HOF96362.1"
                     /translation="MVVKVGIAKLGNIASGVMGELLLDERADREDMITFMATSGTKLQ
                     PEDIDRVVSNMKAWNPDFCIVVSPNGVLPGPTKAREDLAAAKIPCIVITDDVTTKKEQ
                     FDALKASNFGYIIMKADAMIGARREFLDPIEMADYNGNLVKVLAITGAFRKMQQELDK
                     VIDQVKAGKKGADLALPKVVMTVDRAVDGEFSNPYALAKARAAYEIAQSVAGVNVRGC
                     FMTKEWEKYIPMVSSAHEMMRQAMLLCEEAREIEKSTDGVIRKPHKKTGEIVSKTKLI
                     SKPE"
     gene            complement(10533..11459)
                     /locus_tag="PLV90_04280"
     CDS             complement(10533..11459)
                     /locus_tag="PLV90_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HOF96363.1"
                     /translation="MFTIREVRTERGTFQYRRESLTGIRCRISPVRVERKIDTVPALP
                     LSREGCPFCPDSIGVATPTFEDGSRLRYGESVTFPNLYPYAERHIVTVITPDHATGRF
                     GVRCLADALSGAAEALSRSPGYASINWNYLPSAGASIVHPHLQGIADTEPTFLAGLYI
                     AAARRYLADHGRLYWDDLVEHERRSGRFLFEDEIFWSASPVPLGEREVRGVLPVSTLA
                     DIGPYIEPLAGGILRIVEFYRSLGTHAFNVSILFDAPGAGRGHRAFCSLIARLNPNSL
                     SMCDSAFMERLHLEPVILTLPEDLGALFREKG"
     gene            complement(11615..12622)
                     /locus_tag="PLV90_04285"
     CDS             complement(11615..12622)
                     /locus_tag="PLV90_04285"
                     /EC_number="1.5.98.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5,10-methylenetetrahydromethanopterin reductase"
                     /protein_id="HOF96364.1"
                     /translation="MRCIALTTYGIEFVPGAINVKQVVTYTKLAESKDIDYAWITNHY
                     NNRHAYPTLAMIAANTDTIKMGPGIMNTFTDTPAAIASFMATLNEISDGRAVLGIGPG
                     DLSTLPKLAIDPVKPVGHLKEGVEQIRKLLAGEEVKKTGAMEFFDYDGAKLTGVTLPG
                     KKGIPVYIGAQGPKVLELAGTIGDGALINASNPKDFDVAIPIIKKAMEAVGKKTFDVG
                     AYTAMSIDMNEKKARNAAKIVAAFIAAGSPPALLQRHGLDLANVAKIKEALGRFDFKT
                     VGGLVGDKEIDAFTIAGTPDVVKQKCEDLAKAGVTQIIFGSPLGPDMTNSIRLLGKYI
                     V"
     gene            12961..>14437
                     /locus_tag="PLV90_04290"
     CDS             12961..>14437
                     /locus_tag="PLV90_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin/FtsZ family protein"
                     /protein_id="HOF96365.1"
                     /translation="MRVLAIGLGSAGSRVVDNLYDHDRRSRVCCMSAVAIDIDPNSLI
                     QLRHLPDPARVFFPRIDPADQGGVASVIDIEEVMTRIQGMDTMEIDAILLCCGLGGNV
                     ADIAPLVIGELRTSYMEPIFALAVLPCLNEGKRVSAKAADDLDALQALVDAVILFDNE
                     TWSQRITAAATAAGNTGIVDQLRRNAPSGELDPRTRYNLLNERIARQIGLLLRAGEFN
                     ESGLEVAEVVLDAGEVLNTLKGNGLVAVGYAAERLPTGWLDRIYRRRSLKFFIQGSQE
                     KAARIVSLAKKAVYEEISVPCDLTSADKALVLIAGPSTELSMKGFQTVRKWIDRNIAG
                     LEMRSGDYPVKNTSFVGIIIVLSGLANVPRVEELREIRAEYRLECEEEALGAKEEAAR
                     EPVVAPAMREEDIPEFASPIEAAFLKESDPMAEESTAEKDEMIVLTGGGGNNRKSRDE
                     TIDMLPRAVKSQDDGAVILPPKEGGREIDLAGSASVTSSVPA"
     assembly_gap    14438..14537
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14538..14978
                     /locus_tag="PLV90_04295"
     CDS             <14538..14978
                     /locus_tag="PLV90_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HOF96366.1"
                     /translation="GREIDLAGSASVTSSVPAPKNATFGLKGIRIGKTAPKDDDTLFA
                     ASLKPVHRPKDDVLTGDTVVLDRGVRRPRDGTLDEPGLHMRGAAPAPKDAPLDQTGRG
                     FGRGGPHPKEVDPSRGRLDVIDAAERPKKRPEDDRGDDGITWIR"
     gene            complement(14962..15930)
                     /locus_tag="PLV90_04300"
     CDS             complement(14962..15930)
                     /locus_tag="PLV90_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HOF96367.1"
                     /translation="MSPSIHSERRYLELLRILAESHEPLGAKRLSEKMAERGFVLSDR
                     AVQYYLQYLDEMGFTEKVGNRGRILTEAGTAESESALVDERIGFIISRLEQLAFRSTF
                     DPSTGTGSVAYNLSLVREEDLPGVAAAFDEVAEAGYGFLGTYRVVDADPRIPDGHVGL
                     MTVCSVTLDGLLQKRGIPARLEYAGRMIVGENGTAGFLDLIGYRGTSVDPLHLFISAG
                     LTAINRLVTTRTGIALANIRMVPAAARDRVKEAADLMMECGFTFPAGNGIGEFNLPGH
                     PYRLSVVAFSGMNMVANAVEKGYVVRTEIGAGTIPFERMADTTGSR"
     gene            complement(15927..16352)
                     /locus_tag="PLV90_04305"
     CDS             complement(15927..16352)
                     /locus_tag="PLV90_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOF96368.1"
                     /translation="MILSSSEIARRREDGDLVIEPYHPASQQPASYDLRAAGATVLPC
                     GACTLVPSVERVELPADLAATLRCRSSLARRGVLIGGGFVDPGFRGQLTLCLTNMGPE
                     PITLAGGDRVVQMVVQEVRNGDRLYRGRYQDSLGAVHTR"
BASE COUNT         2788 a         5046 c         5125 g         3323 t
ORIGIN      
        1 aagtggctgt agaccacttt catgcggtac tcgtagcctt cgacgacgcc ccggaacatg
       61 cctttcacgt gcgcctcaag cgtgccgctc atggcaagga accgcctctt gtcggatgcc
      121 gtggagacga tgacttcgcc gtcggcgatc ctgacgtcga tctgcgggaa acgcatatca
      181 cggaccagcg tgcctttcgg ccccgatacc gtgagcacgc tgccttcgag tctcgcgttc
      241 acgccgggag ggatctcgat ctgtcgtagt attcccatga gtctctcctc cgtcagtaga
      301 cgtatcccag cagctgcccg ccgatacctt cgccccgggc ctgctcgtgg gagatgactc
      361 ccttcgaggt cgagacgatc aggataccga agttcttcgc ggggaggtac tgcgactccc
      421 agtattccat atcggccata cccaccgaga accggggggt gatggcgcca cacttattga
      481 tcgtcccggg gagctggacc cggaactggc cgccgcggcc gtcgtcgatg aactcgaagc
      541 cgcctataaa gccgttttcc tgcataacgc ggagcattgc gcccaggagc ttgccggcag
      601 gttcaacaat aacctcgctc tttcctgcgt cgccggcatt cttgatcgtg ctcatcgcgt
      661 cagcgattgg attcagtcgt gccatattct ctcacccctt agctcatctt cttaaagccc
      721 atccttccgg cccactcgcg gaagcactgg cgacagaagt agatgccgta tttacgcacc
      781 aggccctgct tccggccgca gatgcggcac tcgttcgcgc cacggccaaa cctctttaca
      841 cccgcgcccg gagcagatgc acctgacttt cctgccatac cttacacctc cacctggtag
      901 cgctcgcgca tgaacgcgat ggcttcttcc tggttcactc tctgctttgc cggcaatttc
      961 ctgcgctcga cgcttctccg tgcaatccgc acgcctttgc gctcgagcac cacgttgacg
     1021 tccatgccga agatgccgat tgccggatcg taggccatgc cggggaagtc cgtgtgctcc
     1081 tcgatgccga aggagacgtt acccgtccgg tcgaactgcg aggcggcaag gtgccgctcg
     1141 acgatggcaa gcgcggtctc gacgaacttc tcggcgtttg cccggcgcag ggtgaccttg
     1201 cacccgatgg gtgcgccctt ccggatgccg aacgccggct gggtccgctt tgcgatggtg
     1261 cggacgggtt tctggccggt gatcttcttc acgagatcct cggccttgac cagacgctcg
     1321 ccgctctctc cgacccccat atgcacgacg accttgtcga tgtagatatc cttcatcgcg
     1381 ctcatgccga ggcctccagt tcgggtgcga tcgcggtgcg gccgaccatg aatatgtact
     1441 cctcgatggt ctcgaaccgc tcgtcggtcg agtcgtccac gaggatcacc cggttcggga
     1501 cggagcttgc ggtcctcgcg atctcgacga tcctcgcgac cttcccggag tgctttccgc
     1561 cgacgaccat ggccacgttg ccttcctcaa agggaaagtg gtcgacgatc cggaaccgtt
     1621 cttcgccgtc cgttccaagg gtcacgacga tggagtcttt tgccctgtag gtgttgtcgg
     1681 caagtacgtt cgcgccgaac gcaaggttca gctgtaccct gccgcccctg acggtggtct
     1741 tattccggat cttgcagagc cgggtctttg cggacgccgc agatatatcg acggcaatca
     1801 ggttgccccg cttgtcgagc tggatgcggt agtgctttgc aagcttcggg atcgagacga
     1861 tgtcaaagac accaaggccc atctgggggt tcctgcaggc ccgcccgttg acgatgacgt
     1921 cgcgctggtg gaggatcttt cgaacctcgc ttgcgttccc tgcaaggccg atatgctcgc
     1981 ggagccagac accgaccggc agggcgccgg cgttgtgggg ccccggagcg gtcttcatga
     2041 cgaacttttg tactttcttc gggatatgcc aggatcccgg cgctaccagc cgcttgagat
     2101 gattggacat tatgctccgc ctccgagtct ctcctcgcgg agtttatctt tcaggttgag
     2161 cttcgtgatc tgcacgttcg agggatcgac cggccgggga acctcggttc catcggattt
     2221 ggtcaccatc acgccgtgca cgatcaaccg gcacatcttc atgtcgaccg catcgacgat
     2281 gccctcttct ttggcgaagt cgccacggag caccttcacg gtatcgcccc tgaccacacg
     2341 ggttctccgg acgctgtact tcccgcgaag ttcgggggag agggatgcgg agaggaagcg
     2401 gccccgggtg tggttcggcg cgttgtaacg cgctttacgc tgttttctcg gttgtttgct
     2461 gactatgcgt accatctttc accacacctt acacgatgat cgtcgccatg gaggcgatct
     2521 ttgggaaccg ttccgcgatc tcgcggggga cagggccctt gatctcggtc cccttcggct
     2581 cgccgcgctc gttgatgagc accatggcgt tgtcctcgaa cgagagccgg atgccgttcg
     2641 gccggcggat ctccttcttc tgccggatga cgaccgcctt ctcaagcttc ctccgcatat
     2701 cgggggtgcc tttcttgacg ctcacggtcg cgacatcgcc gacgcccagg cagggctgtc
     2761 gtcgccggac accgtggtag ccgtcgaccg agacgacctc cacgaggcgt gcgccggtgt
     2821 tgtcggagca gaccatccgg gagccggtgg cgagggcgcg cggaatggtc gactgcatcg
     2881 ccttcattcc ttcatcacct cgaccaccac gaagttcttg gtcttcgaga gcgggcggca
     2941 ctccgcaatc cggaccacat cgccgacgcc cgcgttgatg cagggcgtgc tgtgggcatg
     3001 ataccgggac cggcgcttct cgtatcgctt atatttcttg acgtagtgga ggaactcacg
     3061 ctccacgacg acggttccgt tcatacggtc gctcacgact ttgccggtaa tcacctggcc
     3121 gcgtaccggc aaagtgccgt gaaacggaca atttgcgtca tcacattccg tttccggaac
     3181 ggcgacgtct aacccaatgt ttcgtgccat tattcatcct cattttatgc gcatgctgat
     3241 ccgccgttcc ggctgcatct ccaggctcga gccgtcgacg tcgacggtcg tgccgtccgg
     3301 gagccggatg cggaatacgc tgaaccgctt tggaatccgc gcctctcctc gcccggtctg
     3361 gatgaccagg gtgtttttcg tctcatcgat gatgcgaccg gacaccccga catgacccgg
     3421 gttgctcgcg cgggcgacca gaacatccag gccgatcaac tcgtgccgca ggatgttctg
     3481 gggagagatc atgcagtcct ccgcgcgttc tgctcggtcc ggatacgcgc gatcgtcctc
     3541 cgtatctcgc gtatctgccc gggattctcg gtagcgccgc cggcgctgac tttcccccgc
     3601 tcctggatca gttcgaggga aagtttctgc tcctgttcga ggagctcggt atcggtgagc
     3661 tgtctcactt cgtgtgcccg aaagattgcc atttacctct cctccacgta ctcctcgtcg
     3721 gggatatcct caaactcatc ctcgccgacc tcttcgggct cggcgggcgg aaccggagcc
     3781 tgtttcggcg cgggcgcgag gacgtcgaag gtgtcgggca gccgcgcatc cggcggaacg
     3841 atcttgacct gaaccccgat ggtcccgagt ttcttgacgg cgacggcgta gcctttctcg
     3901 acgatcgtct cgctgggctc gccgcagtgc ttgacgtacc cctcggtaaa cttctgggtc
     3961 cgtgcccggg caccggtcag tttcccggcg atgatgacct cgcagccgag cgcaccggag
     4021 tccatcaccc ggcggatcgt gctctgcccg gccttccgga agtaccagcc gcgttcaagg
     4081 gcgttcgcaa gccgctccgc catgatctgg gcgttgaagt tggggttctg gacctgctgg
     4141 acctccacct gcggcgactc gattgcgtag gcggcagcga gatcctgggt gagctggcgg
     4201 acctgtttgc cgccttttcc gatcacgatg ccgggtttct ccgcaaagat ggtcacctgg
     4261 gtgccgagcg gcgtccgggt gatatccatg ccgccgtagc cggcccgctt gagttccttc
     4321 gtgaggaact tctcgacgcg gacgttcctc acgccgtctg tgataaactt cttctcgatt
     4381 gccattacgc cacctcggtc acgacgatct cgatgttcac ggtctctctg cgcttcgggg
     4441 tggcgcgacc catcgcacgc gggaagaacg cccggagacc acggcccgtg ttcgcggcga
     4501 cgtggtcgat ccggaggttc tcggtatcga ggccgatgta ctcggcgttc tgctcgacgg
     4561 acttaagcag cctgatgtag gcttccgcgg ctttgaccgg gtagcggcct gccgcccacc
     4621 ggtcgaggcc ccgcttgtgg gcgacgttcc ggttgaaccg cttgaagggg atggccttct
     4681 tcagctccac cacatcggca aggtatgccc tggcttcggt ggtggtcatg ttcttgatga
     4741 acctggcaat ctcgatcgag tgtttgggtg agatgggaag ttcgttcgcc tttgcgcgag
     4801 cgacgttctc accctcgata tttgctgagt attcagtcct tgccatagcc atcacttcag
     4861 cggtacgtac ttgctcgacc gggtcgcacc gataccggcg ctgccgtgag agaccctctt
     4921 acgggtcagt gcaaactctc cgaggtagtg aaagaccgcc tcgggctgga tctccacctt
     4981 ctggaactct ttcccgttgt ggatctggat tgtcctcccg accatctcgg gcatgatgac
     5041 catgtcacgc aggtgggtgc ggatggtctc atcccccgac cggacatcgg cgaggagttt
     5101 ctcgtgatcc cgggagagac cccggtcaaa cttcctgcgt gcccgggccg gcatgatcgg
     5161 cagcagctcg gagagagcca tctgctgcag gtccgtaacg gagtggccgc gataggtgaa
     5221 ctcctcacgc cgtcgcggca ttcgcttctg tgtcttcttt gccatgattc acccctcact
     5281 tcttccactt gccggttctc cgggcggcga tatgccctac ctttcttccc ggggatgtcc
     5341 cgcgggatac ggtctttggc cgcccgcagt gctggtgacc gccgccgccg aacgggtggt
     5401 cgatgacgtt catgcagaca cccttgacgc ggggccactt ctctgcggag gatttgacat
     5461 ggaagtgctt ctttcctgcc ttggcgaacg gtttttcgcc ccgtccgccg ccggcaacga
     5521 taccgacggt tgccatgcag acaccgctga accacttgtt cttgccgctc ggcatccgga
     5581 cgccgactct gccgtcgtcg gccttgccga tgacgagggc ctggacgccg cttgcgcgga
     5641 cgaacttgcc gccgtcgttg ggcctggctt cgatgttgca gatgtatgca ccgaccggga
     5701 tctcctggag cggcagggtg tttccgttct tcacggcgcc tcccgacccc caggtgaccg
     5761 tatccccgac accgagccct tcggtggcaa ggacatagat ctttttgccg tcgtcaagcc
     5821 tggtgagggc gatcggcgcg tggcgggcag ggtcgtgctc gatatcgacg acgcgcccgg
     5881 atgtcagggt ggtgttcttc ccggcatgcc ggagctcggc cttgtaccgg tgcgacggtg
     5941 cacgataggt cggaccccct ttaccgcgat tctgcgctat aattctatgt gccattcgct
     6001 actcacctta cattatcccc agccggctga ggatctcttc agccgccttt gcatctgaaa
     6061 acgttacaat ggccttcttc tcgcctttca tcgtcatcat ggtgcggata gcgaccacat
     6121 cctgctcgaa agccgcctcc agctcgcgct tgatctcctg cctggtggcg ttcttctgca
     6181 cgataaactg gagtttgttc tcgccctcga gcatcatcgt tgccttttca gtaacgaacg
     6241 ggtatttcag tatcatgaga gctcctccag cctcctgatt gcagactccg tccagaccgt
     6301 caggcgtcct gcctccgtgc cgggtgcgaa gagctcggcg ttgagctggt cgaccgcaac
     6361 ggcgtcgacg ccggcaaggt tccgggccgc ccggagcggt tcgtctccgg tgacgatcag
     6421 gatgctcttg cgctgcttgt agcggcggcc gcgcatggtg ccgcgccccg cgcgaacctt
     6481 cttgctgccc ttcgcccgct cgacgtcggc gtagacgccg agtgcggaga gcgccgcgat
     6541 gacgtcggcc gtccgggtga cttcctcgaa ccggtcctca aagaccagcg ggaggtcgcc
     6601 ctcgaagagg tgcccgcgcc cacggacgag ttccggggat gtgctggctg cgattgcaga
     6661 tcggagagcc ttctgcttct cttttctgtt gatctctttg acgagaacct tcgcgacctt
     6721 cgggggatgg gctgcacgcc cgccggttgc ctgcggaacc cgggccaccc ggcttccgtt
     6781 cttcaggcgg gggacctgag cgacgcctcg cccgctgccc caggactctg ccgaggtgcg
     6841 gaggcccgcg taggggtctg tcccgtgcgg ctggaaccgg gtgctctgga gcgcgaggac
     6901 tgctcgcttg atcaggtcgg gcctgtactc ctcatcgaat atttccggga gatcgatctc
     6961 gtgggcgatc tcgcctgcca gtgttcgaac ctgtgccttc atcgctgctc acccctgctt
     7021 gctctgcgcg ctgacaaagt tgatcgtcgg cgtgcggacg gtgtgttcac cctgccgtat
     7081 agcgggccgt atccggacaa gacgcttgtt cggcccggga acggaaccct tgatcagcac
     7141 gtaggggccg cgcacgaggc cgtagtggag gaacccgcct tctggtgtta tgtcgtcgcc
     7201 gttcgtgccg atcttcaaga ttcgcttgtt gaactcggtg cgctgctggt agcccatctg
     7261 gcccatctgg ggcacctgcc accggacgtg gtgcgggtgc cacgggccga ggttgccgat
     7321 gtggcgcttc ttgccgcccc gggagtgttt ccgcttccgg acctggacgc cccagcgctt
     7381 tacggggcct tcggtgccct taccggtggt gacggcggtc acgtcgacgt actcgccgac
     7441 cttgaggttc ccggagatga caacctcttt cccgagaacc ccggcggcat aggtgacctg
     7501 gtcgtcgagg cttccgccgg cgatccgcat ctccatcagg tcgggaacct tcttcgggac
     7561 gccggtaagc tccatgggcc gcgtgtaggc cagagcgtag agttccgtga ccctgccctc
     7621 gccgatggcg gccttgatgg cgtcgagggc ggcggcggtg tcgtggttct tcgggacgtt
     7681 gatccgccgc gataactcgt cgtcgagatc ggtcgtccag gcctcggtca gcgcgtgctt
     7741 cccgtaggtg tcctcgcagt atgcgcgcac gcctgccacc cgcatgggcg gaacttcaac
     7801 cacggtcacc ggcaccatca cgtcccggcc ttcggtaggg ctgctcttgt ggtcatccac
     7861 catgatgaca tgggtcattc ccaccttgta gcctgcaaac ccctggacgg ccggggctcc
     7921 cttatactcc ggccatgagc cataccgggg aaccgggctc tttgcccgtt ttcgcgggct
     7981 gtacgcgagg gatcctctgc gtggcctgtt atacttcggc atgtttcaat caacccttct
     8041 tcgtgcgttg tagtctgatg cgccgctctg caggcatgcc gcagggcggt cttccggcga
     8101 acccatgacg ggcagaagat cgagcacccg ggcagatata cccgggcgtg aagggtaaca
     8161 gttgatggag gcaacagagc gtgaggggcg gggagagatc gctctcctgc ttcgtatccc
     8221 atcgctctcc gttgccggcc cgggtgtgcc acccgggtac gctccagtcg tcgccaatag
     8281 atcctgactc tttgacgctt ccggagagag tctccggaac cgggcgcgct gcactggcgt
     8341 tggccccgcg tctcgatcag atccagctcc tgtcatcccg ttgtgacgag cacaactcct
     8401 caatcgagat cggccctcct tgcggagggt tcgtccttcg tctgtttggt acctcctgta
     8461 ctctcctgcc gcgggtctca ggtccgagaa tcttacggat cagagagatt ccggagatcg
     8521 agggcacata acgtgccggc taccccgggg gcccggatag gattatacag ggcggtttct
     8581 taaacattac ttaaaatggg atataaacct tgtcatccgt tctctcacaa aattgcgccg
     8641 aacgggtgct gctgcaggca tgaggccggc accccggacc gaacggccgg atgggtgaat
     8701 gaagggctgc aaggatgctg ctgccggaga agaggcaaaa gatccgtttg tgcggagcac
     8761 tcgctccggc agggccattc ccgcacgagg cccgcgcgtg tgcgcgcccc cgattcgctt
     8821 catccgaagt agagatcgcc gtcctggttg atgtagatct cgacatcgtc gcggacctgc
     8881 ctgcccctga accgatcggg atttgcatcc ctgagcctgt tgatgagcga tatcgtgtcg
     8941 tacttgactt ttacccccat cctctcggct tcggcgtata tgatctcagg agctttgagc
     9001 agatcgaggc ccccctcgag cgtacagagg tgtgcggcat cgagcgtgta gagaaactca
     9061 agcgtctcgc gcgcacgcgc tatgttctca agggctgcct tctcaatggt acagacgttg
     9121 gcctttgagg tctttatgat gtctgcgatc tgctgctgcg tcagtcccct cttccggtag
     9181 cgcaacactt ctttctggcg atcggtaagc agaccttgtt tcatcagaat tatatatgac
     9241 cttgacaaat ttaaacactt ttccttaacc acccccgcgg tcgcaccttt ccggggccat
     9301 ttcaaaatgt ttatatgaga atttggtgat actatcgtgt tctcaaattt tgagaggtgt
     9361 taaaagtggt tgttaaagtt ggaattgcga aactgggaaa tatcgccagc ggtgtaatgg
     9421 gtgaactcct cctcgacgaa cgtgccgacc gtgaggatat gatcaccttc atggcgacct
     9481 ccggcacgaa gctgcagccc gaggacatcg accgcgtggt cagcaacatg aaggcatgga
     9541 accccgactt ctgtatcgtc gtctccccga acggcgttct ccccggaccc accaaggccc
     9601 gtgaggatct cgcagcggcc aaaatcccct gcatcgtcat caccgacgac gtcaccacca
     9661 agaaggagca gttcgacgcg ctcaaggcga gcaacttcgg ctacatcatc atgaaggccg
     9721 acgccatgat cggcgcccgg cgtgaattcc tcgaccccat cgagatggcg gactacaacg
     9781 ggaacctcgt caaggtcctc gccatcaccg gcgcgttccg gaagatgcag caggaactcg
     9841 acaaggtcat cgaccaggtc aaggccggca agaagggcgc agacctcgct ctcccgaagg
     9901 tcgtcatgac cgtcgacagg gccgtcgatg gcgagttcag caacccctac gcgctcgcga
     9961 aggcccgcgc ggcctacgag atcgcgcagt cggttgccgg cgtcaacgtc aggggctgct
    10021 tcatgacgaa ggagtgggag aagtacatcc cgatggtctc aagcgcccac gagatgatgc
    10081 gccaggccat gctcctctgc gaggaggctc gcgagatcga gaagtcgacg gacggcgtca
    10141 tcaggaagcc ccacaagaag accggcgaga tcgtctcgaa gacaaaactc atcagcaagc
    10201 ccgagtaaac ccaacaccat cccttttttc gaccctggag tgggtgatcg gaccgcgccc
    10261 cgccatccgg gttgccggga accaccggcg ccgtgccggg gtgggttgtg gggcagaaga
    10321 gcggccccgc gcaggtgcct ccggcgaccc gcgctctgca catttgcaca tgcgctgcag
    10381 cctcagagat cgtcggctcc gcgatggtcc ggatgtcgcg cgggtcgccg acggtcaggg
    10441 tacggacccg gtcgaccgcc tcccgggtat aggggaaccg gacgtcgagg atcccggcgc
    10501 ggaatcccca cggttaaggc tcaacgccag aatcaaccct tttcccggaa gagcgctccc
    10561 aggtcctcgg ggagcgtcag gatgaccggt tccaggtgca gccgctccat gaacgccgag
    10621 tcgcacatgg agaggctgtt cgggtttaac cgcgcgatga gggagcagaa ggcccggtgg
    10681 ccccgccccg ccccgggggc gtcaaagagg atcgagacgt tgaaggcgtg cgtcccgagg
    10741 ctccggtaga attcgacgat ccggaggatc ccgccggcca gcggctcgat gtagggcccg
    10801 atatcggcaa gcgtcgatac cgggagaacc ccccgcacct cccgctcgcc gagggggacg
    10861 gggcttgccg accagaagat ctcgtcctca aagaggaacc ggccggagcg gcgctcgtgc
    10921 tccaccaggt cgtcccagta gaggcgaccg tgatcggcga ggtaccggcg agcggcggcg
    10981 atgtagaggc ccgcaaggaa ggtgggttcc gtatcggcga taccctgcag gtgcgggtgg
    11041 acgatgctcg caccggctga agggaggtag ttccagttga tgctcgcgta gccgggagac
    11101 cgggataggg cttctgccgc gccggatagg gcgtcggcga ggcaccggac gccgaaccgg
    11161 ccggttgcgt gatcgggcgt gatcaccgtg actatgtggc gctccgcgta ggggtagagg
    11221 ttcgggaacg tcacgctctc cccgtaccgg aggcggctgc cgtcctcgaa cgtcggggtg
    11281 gcgacgccta tcgagtcggg gcagaacggg cagccttcgc gagagagagg gagggcggga
    11341 acggtatcga tcttacgctc gacccggacg gggctgatcc ggcaccggat gccggtgagg
    11401 gactcccggc ggtactggaa ggttcctcgt tccgtcctga cctcgcggat ggtgaacatg
    11461 acccttcgta ctccgattgg cgcgcctatc gtataaagcc ctgtcagggt tatcggtggc
    11521 ggagaccgcg gaggaggtac gcgatagagg cccgcgcgtg cccttcgtcg gggccagaac
    11581 cgttcaagaa aaagtggtga gggttgtttc tgccttacac gatgtacttg ccgagcaggc
    11641 ggatggagtt ggtcatgtcg gggccgaggg gcgagccgaa gatgatctgg gtaactccag
    11701 cctttgcgag gtcttcgcac ttctgcttga cgacatcggg ggttccggcg atggtgaagg
    11761 cgtcgatctc cttgtcgccg acgagtccgc cgacggtctt gaagtcaaag cgtccaaggg
    11821 cttccttgat cttcgcgacg ttggcgaggt cgaggccgtg gcgctgcagg agcgcgggcg
    11881 gggagccggc ggcgatgaat gcggctacga tctttgcggc gttccgggcc ttcttctcgt
    11941 tcatgtcgat ggacatggcg gtgtaggcgc cgacgtcgaa ggtcttcttg ccgactgctt
    12001 ccattgcctt cttgatgatc gggatggcga cgtcgaagtc cttggggttc gaggcgttga
    12061 tcagggcgcc gtcgccgatc gttccggcga gctcgaggac cttggggccc tgggcgccga
    12121 tgtagacggg gatgcccttc ttgccgggca gggtgacgcc ggtcagcttg gcgccgtcgt
    12181 agtcgaagaa ctccatcgcg ccggtcttct tgacctcttc gccggcaagg agcttgcgga
    12241 tctgctcgac gccttccttc aggtggccga cgggcttgac ggggtcgatg gcgagcttcg
    12301 gaagcgtcga gaggtcgccg ggaccgatgc cgaggacggc acgtccgtcg gagatctcgt
    12361 ttaaggtagc catgaaggac gcgatggctg ccggggtgtc ggtgaacgtg ttcatgatgc
    12421 ccggtcccat cttgatcgtg tcggtattcg ccgcgatcat ggcgagggtc gggtatgcat
    12481 gacggttgtt gtagtggttg gtgatccagg catagtcgat atccttcgac tctgcgagct
    12541 tggtgtaggt caccacctgc ttgacgttga ttgctccggg cacaaattca attccatatg
    12601 tagtcaaggc tatgcacctc attgagtagt tacgtgcagg gagttatata tattaccatt
    12661 ttgtgctcgt gaaacgaggt gtaccgcaca tgcaaggcaa aacagaggga tgtaacaggt
    12721 ttataaggat atccgatgaa tcctctccct taccctgcag gcggcgcaga tacacgggaa
    12781 gagaaccgga atgttcaaat atagaacgtt tgtgaacgta tgcgctccgg gatcccgggg
    12841 acgtacagaa atatataagt ttgtctgccc acatactcat cagtagttcc ggatgatatg
    12901 cgcgcttttc ctcgcgtcaa cggtcatccc gggcatgcga aggttcgagg gtataacgat
    12961 atgcgggtgc tggcgatcgg actcgggagc gcggggtcgc gagttgtgga caacctctat
    13021 gaccacgaca ggcggagcag agtctgctgc atgagtgcgg tggcgatcga tatcgacccg
    13081 aattcgctga tccagctccg ccatctcccg gacccggcaa gggtcttctt cccgcggatc
    13141 gatccggcgg accagggcgg ggtcgcgagc gtgatcgaca tcgaggaggt catgacccgc
    13201 atccagggca tggatacgat ggagatcgat gccatcctgc tctgctgcgg gctcgggggc
    13261 aacgtcgccg atatcgcacc gctcgtcatc ggcgagctcc ggacgtctta catggagccg
    13321 atcttcgccc tcgccgtcct gccgtgcctc aacgagggaa agcgggtctc ggcaaaagcc
    13381 gccgacgacc tcgacgcact tcaggcgctt gtcgatgccg tcatcctctt cgataacgag
    13441 acctggtcgc agaggatcac ggcggccgcg accgcagccg ggaacaccgg gatcgtggac
    13501 cagctgcgca gaaatgcacc ttcaggagaa ctcgatccgc ggacccgcta caacctgctc
    13561 aacgagcgga ttgcccggca gatcggcctc ctcctccgcg ccggcgagtt caacgagtcc
    13621 gggctcgagg tcgccgaggt cgtcctggac gccggagagg tgctcaacac cctgaaaggg
    13681 aacggtctcg ttgcggtcgg ctacgctgcc gaacgcctgc cgaccgggtg gctggaccgc
    13741 atctaccggc ggcggtcgct gaagttcttc atccagggct ctcaggagaa agcggcccgg
    13801 atcgtctcgc tcgcaaagaa agcggtttac gaggagatat cggtcccctg cgacctcacg
    13861 agcgccgaca aggccctcgt cctgatcgca gggccttcta ccgagctctc gatgaagggg
    13921 tttcagacgg tcaggaagtg gatcgaccgg aacatcgccg gcctcgagat gcgttccggc
    13981 gactacccgg tgaagaacac atcgtttgtc gggatcatca tcgtgctctc ggggctcgca
    14041 aacgtccccc gcgtcgagga actcagggag atccgggccg agtatcgtct cgagtgcgag
    14101 gaggaggcgc tgggagcaaa agaggaggcg gccagggagc cggtggtcgc cccggcgatg
    14161 cgggaggaag atatcccgga gttcgcgtcg cccatcgagg cggcatttct caaggagagt
    14221 gaccctatgg cagaagagtc tacagcagaa aaggacgaga tgatcgtgct tacgggcggc
    14281 ggtggcaaca acagaaagag cagggacgag accatcgata tgctcccgag agccgtgaag
    14341 agtcaggacg acggcgccgt catccttccc ccgaaagagg gcgggaggga gatcgacctt
    14401 gccgggtcgg catcggtcac atcgtccgtg cctgcgcnnn nnnnnnnnnn nnnnnnnnnn
    14461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14521 nnnnnnnnnn nnnnnnnggg agggagatcg accttgccgg gtcggcatcg gtcacatcgt
    14581 ccgtgcctgc gccgaagaac gccaccttcg ggctgaaggg gatccgcatc gggaagacgg
    14641 ctccaaagga tgacgatacg ctcttcgccg catccttaaa acctgtgcac cgcccgaaag
    14701 acgatgtgct caccggcgat acggtcgtgc tggaccgcgg ggtgcggcgg ccgcgcgacg
    14761 ggaccctcga tgaacccggc ctgcatatgc gcggggcggc tcccgcgccg aaggatgccc
    14821 cgctggacca gaccggccgg gggttcggcc gcgggggacc ccacccaaag gaggtggatc
    14881 cctcccgcgg aaggctcgac gtgatcgatg cggccgaacg gccgaagaag agaccggaag
    14941 acgaccgcgg cgacgacggg atcacctgga tccggtagta tccgccatcc gctcaaacgg
    15001 tatggttccg gcgccgatct cggtcctgac gacgtagcct ttctcaacgg cgtttgccac
    15061 catgttcatg ccgctgaagg cgacgacgga gagccggtag gggtgcccgg ggaggttgaa
    15121 ctccccgatc ccgttcccgg cggggaaggt gaacccgcac tccatcatca ggtcggcagc
    15181 ctcttttacc cggtcccgcg cggccgccgg gaccatacgg atgtttgcaa gcgcgatacc
    15241 ggtccgggtc gtcaccagcc ggttgatcgc ggtgaggccc gcggatatga agaggtggag
    15301 cgggtcgacc gacgtccccc ggtagccgat gaggtcaaga aacccggcgg tgccgttctc
    15361 gccgacaatc atccttcccg cgtactcaag cctcgcgggt atccccctct tctggaggag
    15421 accatccagg gtgacgctgc agaccgtcat gagcccgacg tggccgtcgg gtatccgggg
    15481 gtcggcatcg acgacccggt aggtgccgag gaacccgtag ccggcctccg ccacctcgtc
    15541 gaaggccgcc gcaacgcccg ggaggtcctc ctcccgcacg agggagaggt tgtaggcgac
    15601 gctcccggtc ccggtcgacg ggtcgaaggt gctccggaag gcgagctgtt cgagcctcga
    15661 tatgatgaaa ccgatccgtt cgtcgacgag cgcgctctcg ctctcggcgg tccctgcctc
    15721 ggtgaggata cgcccccggt ttccgacctt ctccgtgaac cccatctcgt cgaggtactg
    15781 gaggtagtac tggacggcgc ggtcgctcag gacgaacccc cgttcggcca tcttctcgct
    15841 taacctcttc gcacccaggg gttcgtgcga ttccgcgagg atccggagga gttcgaggta
    15901 tctccgctcg gaatgtatgg atgggctcat cgcgtatgca ccgcgccaag gctgtcctgg
    15961 taccggcccc ggtagagccg gtcgccgttc ctgacttcct ggacgaccat ctggacgacc
    16021 cggtcccctc cggcgagggt tatgggttcc gggcccatgt tggtcaggca gagggtcagc
    16081 tggccacgga aaccgggatc gacgaacccg ccgccgatga ggacgccacg gcgggcaagc
    16141 gaggagcggc accggagggt tgccgcgagg tcggcgggga gttccacccg ctcgaccgag
    16201 gggacgaggg tgcatgcgcc gcacgggagc acggttgccc cggccgcgcg gaggtcgtac
    16261 gatgcgggct gctgggatgc ggggtggtac ggttcgatga cgagatcgcc gtcctcgcgc
    16321 cggcgagcga tttcgctgga tgagaggatc atcgtatagt gtactggcat aaaaacgctt
    16381 aa
//
LOCUS       DAOSII010000036        16282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_3741_length_16282_cov_41.871264, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000036 DAOSII010000000
VERSION     DAOSII010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 16282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16282
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3741_length_16282_cov_41.871264"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..1208
                     /locus_tag="PLV90_04310"
     CDS             30..1208
                     /locus_tag="PLV90_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HOF96369.1"
                     /translation="MKVCIMCGGEGTRLRPLTFGRPKPCIPIVNKPSIQHLVSHLANL
                     GFNDVIITLGYMSDAIEKALGDGSLFGVNVTYVHEKTKLGTAGSVKNAQRYLEEQPFL
                     VVGGDHVVSLNLLEFYREHLANDSLTTIGLISIDDPTEYGIAEIDANYQIKRFKEKPG
                     PGEIFSNLASTGMYVCDPEIFDHIPTGEKFDFARNLFPKLMEEGHILKAWLARGNWSD
                     VGSPRSLREAERWKLQEIGFTNISGDLYIKGARIMGPAQIGSCVSVAANSRVIGPVSI
                     GAGTIIEENVVIGPYTSIGEGCIIQNSAKIFSSSIYNRVVVGRDSTVSGSIIDNDTLI
                     GSGCNIEHDTVIGPHVVLKGGAIVHSGTRIWPKVIIPEGTVVKEHFLNEEYDTRTEGS
                     "
     gene            complement(1205..1993)
                     /locus_tag="PLV90_04315"
     CDS             complement(1205..1993)
                     /locus_tag="PLV90_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOF96370.1"
                     /translation="MDKKRVKDYMTYDVVTVDAHGTVRDVIETIKRTRHDGFPVVENS
                     KEVVGYISARDLLFAHPATPVEQVMSRHLIVADPDMSINDAARVIFRSGIQKLPVVDE
                     KNELIGIISNADVIRSQIEHVSPDKVFKFIETLRKLYGVEPTLRRGPVPINDLLPTQS
                     KIYEDELEGRMYEIKKGLAEPLIVVRRPGRWILVDGHHRAIAAKRLGIKNLDAYIIEV
                     RENIELGMERTAKTLNLNTLDDIKVLDYARHPLVALTHRLVRHG"
     gene            complement(2058..2528)
                     /locus_tag="PLV90_04320"
     CDS             complement(2058..2528)
                     /locus_tag="PLV90_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOF96371.1"
                     /translation="MARIDRGPYRTIWQDFDDLMAEMENRFQSMLGGIGARGEEIRGR
                     VVPAVRDFRVDVRDHEDEVIVVADLPGVEKEDVAVRITDPLHLEIASTRTSETGEEGQ
                     DFFMRERIYGQMSRMVLLPAEVTEENAAASFKNGVLEVRLKKVPAEIGTTIPID"
     gene            3049..4338
                     /locus_tag="PLV90_04325"
     CDS             3049..4338
                     /locus_tag="PLV90_04325"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF96372.1"
                     /translation="MKISEGDRVVFALSFARMGDAIGNSILIIVIPLLTAQLPAPLFP
                     IPGPARVGVLIAIYGLLAALVQPISGVLIDRTNRHKLFILVGLAMVAIATFGYSFATL
                     FESLLGLRILQGIGFALTIPASVAIVTIVTQKMTRGGSMGIYNSSRFLGFAIGALIGG
                     YLYTTYGPNMAFYVGTGFIILAIVLVQAFVKEVEEPEEPRDDPEVPAGPIHQEENAAF
                     EGGKRTLPGLRGLGFISAGIGGLAFAVFLMASAFAMLVTLEPQFNIRLHETAFAFSIA
                     FSTLAATQVVFQYPIGRVSDRWGRKPFVIAGLLLIAPTTAALGFISTTFQLVSLRFIQ
                     GIATAAIAAPAFALAGDLSKRGTQGRQLSVVTTGFGLGIALGPLIAGFLVVYGFEVPF
                     LVGAALSLLGAWVVYRYVPETVEREPGARSFRWPGRR"
     gene            4417..5295
                     /gene="cyoE"
                     /locus_tag="PLV90_04330"
     CDS             4417..5295
                     /gene="cyoE"
                     /locus_tag="PLV90_04330"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HOF96373.1"
                     /translation="MEHRGVLPAYLELTKPKVVFLNLFVGLVSFLLAASHPPPVHLCT
                     FLAAGYLVAGGCGAINCWYDRDIDRVMARTSGRVIPRNALSPRRVLLFGSLLLTTGLL
                     AAVLILPFLAAAMMAAGAGIYLIIYTAWLKRASHLNVVLGGTAVCFAAFAGWTATGAP
                     FPTLTPVLIALVGFLWTPGHFWALAIYRDQDYRIARIPMLPARAGRYPAAQAIFLWNA
                     ATAGLTVLIPFLTAIDRITLAITIPAGLFLLSLSRRLLVSPSDERAVRLFSFSIVYLM
                     SVLFAFLVLADGLPAL"
     gene            complement(5332..6078)
                     /locus_tag="PLV90_04335"
     CDS             complement(5332..6078)
                     /locus_tag="PLV90_04335"
                     /inference="COORDINATES: protein motif:HMM:NF021214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HOF96374.1"
                     /translation="MEGNGKADRGRTGRSTKIFASFTAVATLIAMAYLNPALGESFTI
                     HMVYHMILIGVLAPSLLAADFFLWWLPPGTLRKWALYRTLRRGLYAISYPVTAFILST
                     AVLWFWHIPIPYDITLTDMPVHILEHVTLLIAFIAYWAPLVPGSRLHLPVIRTNEGKA
                     LYLLAGAMQGMILGAIITFQDQIVYLYPSTAHLPGVTLLGDQEMGGAAMWFIGAIIYA
                     VAAILSFRSTDPDIHRDVPPARGAIGGQEE"
     gene            complement(6082..7593)
                     /locus_tag="PLV90_04340"
     CDS             complement(6082..7593)
                     /locus_tag="PLV90_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HOF96375.1"
                     /translation="MSSDAVPPVKTPSLPANYGGVLRFTLTGMVFAFVMFVIMGIFGL
                     TMRTEQSGLLNLLGPELFYRIMTLHGLSMVSLALLGSIAGFAAVIGRRVVLDTRWLWA
                     TFFFFFIGMMFVLFSTLGGGFAAAWTALYPLPFHGGYAFWGVIGATVGIGFVLVGLLF
                     YCVLITRTVSRAHGGVINALGWPLIVRRTARQENLSILDVLAVAVAIPGIVAVIAGFT
                     WLIPLWLQASGLIQSIDVLFMKNFDYLFGHLLVNLTIYFAAGLMYFLLPAYTGRAWKL
                     SRTYALALNAITFIVPIIYLHHLYQDFAQPLAFQVAGQIGTYVSVIPVILITLFGGLS
                     QYYRSGIRWDVTTIFLAIGLWGWMFGGMAAALDATIAVNQVMHNTLWIPGHFHTYFLL
                     GAVVFLWGFLFSILRTLSGAGSSPRTRYAAAAYGVGGAGFTLMFLASGASSIPRRYAV
                     HFPEWQIFASIAVPFIILIGVGVIWIGYVMLSRVPRAWERTRGPVDILPEGGS"
     gene            complement(7583..8116)
                     /locus_tag="PLV90_04345"
     CDS             complement(7583..8116)
                     /locus_tag="PLV90_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96376.1"
                     /translation="MIFIDPRYVFVLLLVMGGSYTLLWAWVMRSSREKVGYDDITEGL
                     ELLRRRLYSVIVLAAVVVFLGTQVALPYQPVRDITVGEPVLVINATTSQFAFTLSNTT
                     IPANVPVEFAVTSIDVNHGFGIFDPDGRIIAQTQSMPGYTNRLIVNFTRPGPYRIHCL
                     EYCGVDHWGMEAAFNVE"
     gene            8384..8821
                     /locus_tag="PLV90_04350"
     CDS             8384..8821
                     /locus_tag="PLV90_04350"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOF96377.1"
                     /translation="MAPVTDGDTLLLHFTSTRPDGEIFEDTRAGEPVRATLGERQINP
                     LFEEALIGREPGETVTVVLPPEKAYGKYRSRLVITMKRKRLNLDHEPVPGEFLKVEVM
                     GKPCLVTVTDVTEKSVTIDANHPLAGETITYAITVAAILPRSG"
     gene            8989..9657
                     /locus_tag="PLV90_04355"
     CDS             8989..9657
                     /locus_tag="PLV90_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOF96378.1"
                     /translation="MKIVGINGSPRGGRSATLQLIEAVLEGAKEKGAEVDVVHLIDLD
                     LKFRNACSACFQEGKCVDEDDFPNLYEKIKAADGLVLGSPVYFDLVTGQMKLLIDRMA
                     DGIQCQSLTGKYGCSVATSGDHAEPAVVAYLNHFLQMLGATPVGNVGVAIGKDPGALA
                     KAKETAHELGKTLAEAIQTRRQYPQIEAFHKRFQEKFKAVIAGEKPEWPGDYDHWVNQ
                     AWVW"
     gene            9869..10183
                     /locus_tag="PLV90_04360"
     CDS             9869..10183
                     /locus_tag="PLV90_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOF96379.1"
                     /translation="MPEFANPFSGNRMERRLDRGELIRTIRFSIAAEYEAVQFYEQIA
                     ESTDDPLVQKVMRDVANEEKEHAGEFLRLLREIEPTEDGFYQHGYKEVEEMIAEVKKG
                     GR"
     assembly_gap    10245..10344
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10582..10983
                     /locus_tag="PLV90_04365"
     CDS             10582..10983
                     /locus_tag="PLV90_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOF96380.1"
                     /translation="MKKGFVTDIETETVKNTDFRRVLYTGKFSQLVLMCLKPGEEIGA
                     EVHDDVDQFFRFEEGEGAVVIDGVKHAVRDGSAVVVPSGANHNVLNTSKTADLKLYTI
                     YSPPEHQDKVVRKTREEAMAREEHFDGKTTE"
     gene            complement(11339..11749)
                     /locus_tag="PLV90_04370"
     CDS             complement(11339..11749)
                     /locus_tag="PLV90_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOF96381.1"
                     /translation="MIEDLERQGLIQRLPPDPKKVADAMALARRDIDTARTLLSTDCD
                     WAYTIAYNAALQAGRALMFAKGYRPDGANQHISVVKFAGLFLSEGDALVFDRMRRKRH
                     SSVYDTVGTISAAEAEFAVSAAVALVQKVTTLLG"
     gene            complement(11733..12308)
                     /locus_tag="PLV90_04375"
     CDS             complement(11733..12308)
                     /locus_tag="PLV90_04375"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOF96382.1"
                     /translation="MLEALIPSKARVKLLTLFLLNPESEFYIREIVRMTGENINGVRR
                     ELANLESFGLLIGRRRGNQHYFTVNRDFFLYTDLQQLVLKTEGVARVIRENLSSLQNI
                     ECMFIYGSFARGTAGGRSDIDLFIVGDVNEEVLIPLVHTGERAINREINYTLMRGSEF
                     AQRRETGDPFVKNVMGERKIMIIGSCDDRGS"
     gene            complement(12503..13912)
                     /locus_tag="PLV90_04380"
     CDS             complement(12503..13912)
                     /locus_tag="PLV90_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF96383.1"
                     /translation="MIREFVDRETERALLEEEWQKDGGRLIILYGRRRIGKTRLVTEF
                     VRGKEGILHFAEDTAPQIQIRRLQEAIARSFADPLLATLDITTWDQLFTYITRIPLTE
                     RRYLVIDEFIYLIKNDPTILSALQKAWDTALAGSNWCILLSGSMLGLMTDSALSYTSP
                     LYGRRTRDMLLGPLRFRDARQFLPSFSFGDALKAYLTIGGIPEYLLKAGDYRSYETFV
                     EREFFNRYGYFYREPYFLLSQEFRELKTYQAILNAIAVGNTAPGTIAQFCGMDARHLY
                     PYLEAMTRLRIIERELPLLASRRQGLYRIEDCIFDFWYNFVFSHRQAIEMENHPIDTI
                     DTNPYFGKQFEVFMRNELARAAFPGYTIGRWWYRGDEVDLVATRESDASIVFGECKWG
                     DLTEREARGVLTRLVQKAERIRHDRYTHERFCLVAGGVEGKGRLREEGFLVYDLADVE
                     AACSAGQAPARTYSSPEER"
     gene            14075..15274
                     /locus_tag="PLV90_04385"
     CDS             14075..15274
                     /locus_tag="PLV90_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF96384.1"
                     /translation="MSSPPQRMIRRDAEAQIRSLALGFPAVAVIGPRQSGKTTLVRSV
                     FPHLPYVLLEDPDTRAFAEEDPRSFLIHYEKTGAILDEVQRVPELFSYLQGVLDANQR
                     PGQFILTGSQNFLMMERISQSLAGRVGIVKLLPLSMGELARAGLEAERYEDLLYAGLF
                     PRPYSSAVHPRDFYSSYIQTYIERDLRLLKQVQNLSAFQTFMKMCAYRSGQVVNYSSL
                     AHDCGITHNTAKEWLSLLETSMLIVQVRPHRKNFNKRLVRMPKLYFTDPGLAAHLAGV
                     QGADDLIYHPLRGGLFESLIIAEFLKFRLNRGKESNLYFWRDKLGHEIDCIIEYMGWD
                     PIPVEIKSGRTPSPDFFNEIAYWNELSGNAPDRSFVVYGGDRSQRRAAGHLVGYRDLE
                     PIIRYLE"
     gene            complement(15384..16094)
                     /locus_tag="PLV90_04390"
     CDS             complement(15384..16094)
                     /locus_tag="PLV90_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96385.1"
                     /translation="MEEQVGRLLDDLDFPALYRGYTWRDDTWERGFLEILDLEREVTA
                     AARSLALGLDHVQKIARRDDLSAPARIAVTLYIGSAPAYWLMLEPEETIQTVERQIRG
                     LGPVCASKLLRFAVPQVFGTLDARLIRVFGRGDSGSQRYPLLDLAVEPSGDRWAIPAG
                     QPGWPGEYGAWAGALQAVAGGLNRDEVSCPHPAGFAAAGLRSEGIWAAADVEMALSCY
                     AAGVLRGEERKDSVTGVV"
     gene            complement(16094..>16282)
                     /locus_tag="PLV90_04395"
     CDS             complement(16094..>16282)
                     /locus_tag="PLV90_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96386.1"
                     /translation="DAVGDLRRGDIVRFTAAIDGYVKGYRREKIDDRLMRPPAVDYRL
                     KFPRRAERIGGAPSPEVR"
BASE COUNT         3125 a         5008 c         5055 g         2994 t
ORIGIN      
        1 ttaaatcgat acgcagacaa ccgtgtctca tgaaggtgtg cataatgtgc ggaggggagg
       61 gcacacggct ccggcccctc acattcgggc gtcccaaacc ctgtattccc atcgtcaaca
      121 aaccctctat ccagcacctg gtctcacacc tcgcaaacct cgggttcaac gacgtgatca
      181 tcacgctcgg ctacatgagc gatgcgatcg agaaagcgct cggggacggg tcgctctttg
      241 gcgtcaacgt cacctacgtc cacgagaaga cgaagctcgg gacggcgggc agcgtcaaga
      301 acgcccagag atatctcgaa gagcagccgt tcctcgtcgt cggcggcgac cacgtggtga
      361 gcctgaacct gcttgagttc taccgcgagc acctggcaaa cgactcgctc accaccatcg
      421 gcctcatcag catcgacgac cccaccgagt acgggattgc cgagatcgac gcaaactacc
      481 agatcaagcg gttcaaggag aagccggggc cgggagagat cttttcaaac ctcgcgagca
      541 ccggcatgta cgtctgcgac ccggagatat tcgaccatat accgaccggc gagaagttcg
      601 actttgcccg aaacctcttc cccaaactca tggaggaggg gcatatcctg aaggcctggc
      661 ttgcccgggg caactggtcc gacgtcggga gcccgcggtc gctccgggag gcggagcggt
      721 ggaaactcca ggagatcggg ttcaccaaca tatccggcga cctctacatc aagggcgccc
      781 ggatcatggg cccggcgcag atagggagct gcgtctcggt tgcggcgaac tcccgggtga
      841 tcgggccggt ctcgataggt gcagggacga tcatcgagga gaacgtcgtc atcgggccgt
      901 atacgagcat cggtgaaggg tgcatcatcc agaacagcgc aaagatcttc tcctcgtcca
      961 tctacaaccg ggtggtggtc gggagggaca gcaccgtcag cggaagcatc atcgacaacg
     1021 atacgctcat cggcagcggg tgcaacatcg agcacgatac ggtcatcggc ccgcacgtcg
     1081 tcctgaaagg cggtgcgatc gtccactccg ggacccggat ctggccaaaa gtcatcatac
     1141 cggaggggac ggtggtgaaa gagcacttct taaacgagga gtacgacacc cggaccgaag
     1201 ggtcctaacc gtgccggaca agccggtggg tcagcgcgac gaggggatgg cgggcgtaat
     1261 cgagcacctt gatgtcgtcg agggtgttga ggttcagggt ctttgccgtc cgctccatcc
     1321 ctaactcgat gttctcgcgg acttcgatga tgtaggcgtc caggttcttg ataccgaggc
     1381 gtttggctgc gatcgcccgg tggtggccgt cgaccaggat ccagcggccc ggcctccgaa
     1441 caacgatcag gggttcggcc agccccttct tgatctcgta catccgtcct tcgagttcgt
     1501 cctcgtatat cttggattgg gtggggagga ggtcgttgat cgggaccggg cctcttcgca
     1561 gcgtcggttc gacgccgtag agtttccgta aggtctcgat gaacttgaag accttgtccg
     1621 gcgagacgtg ctcgatctgg gaccggatga cgtcggcgtt cgatatgatg ccgatgagct
     1681 cgttcttctc gtcgacgacc ggaagcttct ggatgccgga ccggaagatg acccgggcgg
     1741 cgtcgtttat gctcatgtcc gggtcggcga cgatgaggtg gcgggacatc acctgctcta
     1801 ccggtgttgc gggatgggca aagaggaggt cccgtgccga tatgtagccg accacttcct
     1861 ttgagttctc cacgacaggg aacccgtcgt ggcgcgtcct ctttatggtc tcgatgacgt
     1921 cgcggaccgt cccgtgggcg tcgacggtga cgacgtcgta ggtcatgtag tccttgaccc
     1981 tctttttgtc catcatgata gtgtctcgtt cgcccgagat gaattgttcg gtcgagagcg
     2041 gcgggcgcaa aaaagggtta gtcgatggga atcgtggtcc cgatctcggc cggcaccttc
     2101 ttcaggcgga cctcgaggac gccgttcttg aacgaggcgg cggcgttctc ctcggtcact
     2161 tcggcgggga gcagcaccat gcggctcatc tggccgtaga tgcgctcccg catgaagaag
     2221 tcctggcctt cctctccggt ctcgctcgtc cgcgtgcttg cgatctccag gtgcagggga
     2281 tcggtgatcc ggacggcgac gtcctctttc tcgacgccgg ggagatcggc gacgacgatc
     2341 acctcgtcct cgtggtctct gacatccacg cgaaagtcgc ggaccgccgg gacgacccgg
     2401 cccctgatct cttcgcctcg cgcgccgatc cccccgagca tggactggaa cctgttctcc
     2461 atctcggcca tgaggtcgtc gaagtcctgc catatcgtgc gatacggtcc tcggtcaatt
     2521 cttgccatgg tccttactcc tgatcgtccg ggatgcccgg gggcatcctg gctaacctta
     2581 aggtagggtt ttaaatatat aaatatattt catggggcat ccgggtgcac acggttcctc
     2641 tcccgtatgg accgcctgca gggggtctcg cccggcccgc cggatgccct gcgaccgccg
     2701 ccgagcactt tgccccacac attccgtttc cgcccggcgc acggcctctc cccggcaccc
     2761 ccccacccgc acgggcggcc ggtgcctcca tccgacgagg gaggagagag gcgaactccc
     2821 cggtggagga gtgccgatcc ggggtggtgc cggcccatcg ggcggcgtgg cagggctccc
     2881 gtggaaggat cgccgcccga taggccgatc tccggtaaaa agagggttct gcccggaggg
     2941 agggaagtat tttatcgggc aggtgccttg agagagcctc tcgaccggac ataatgagtg
     3001 tgccgcggag aggagagggg gatgacttcc ggggataggg ttctggggat gaagatctct
     3061 gagggagacc gcgtggtgtt cgccctctcg ttcgcacgga tgggggatgc cataggaaac
     3121 tccatcctga tcatcgtcat ccccctcctg accgcgcagt tgccggcccc cctcttcccc
     3181 atacccgggc ccgcacgggt cggcgtattg atcgcgatct acgggctcct ggcagcactc
     3241 gtccagccca tcagcggggt cctcatcgat cgcacgaacc ggcacaaact cttcatcctg
     3301 gtcggtctcg ccatggtggc tatcgccacc ttcggctact cctttgccac cctgtttgag
     3361 agcctgctgg ggctgcgcat actccagggt atcgggtttg cgctgacgat cccggcatcc
     3421 gttgcgatcg tcacgatcgt gacgcagaag atgacccgcg gcggatcgat gggcatatac
     3481 aacagctcgc gatttctggg ctttgccata ggcgccctga tcggggggta tctctacacc
     3541 acctacgggc cgaatatggc tttttatgtc ggtacgggct tcatcatcct cgccatcgtc
     3601 ctggtgcagg ccttcgtgaa ggaggtggag gagccagaag agccccggga tgacccggag
     3661 gtcccggccg gccccataca ccaggaagag aatgcggctt tcgaaggcgg aaagagaacc
     3721 ctgcccggcc tccggggcct cggctttata tccgcgggga tcggcggcct tgcgtttgca
     3781 gtctttctca tggccagcgc attcgccatg ctggtcaccc tggagccgca gttcaacata
     3841 cgcctgcacg agacggcgtt tgccttctcc atcgcgttct ccacccttgc cgcaacccag
     3901 gtggtgttcc agtaccccat cgggagggtg tcggaccggt gggggcgaaa accgttcgtc
     3961 atcgcaggcc tcctgctcat cgccccgacg accgccgccc tcggatttat atctacaacc
     4021 ttccagctcg tcagcctgcg gtttatacag gggatcgcca ccgccgccat cgcggcgccg
     4081 gcattcgcac tcgccggcga cctctcgaag agagggacgc agggccgcca gctcagcgtc
     4141 gtcaccaccg ggttcggcct cggcatcgcg ctcggcccgc tgattgcagg gtttctcgtc
     4201 gtgtacgggt tcgaggtgcc cttcctggtc ggggccgccc tctcgctcct cggggcatgg
     4261 gtggtctacc gctacgtgcc cgagaccgtg gagagggaac ccggcgcccg gtcattccgg
     4321 tggccgggga ggagatagcg cgagggagtt ccactcgccc tcatccaccg gggataacta
     4381 aatatgggaa ggtatcacga gggttgccgg agatcgatgg agcatcgggg agtactgccg
     4441 gcatacctgg agctgaccaa accgaaggtg gtattcctga atctcttcgt aggattggtc
     4501 agtttcctcc ttgctgcatc gcacccgccc cccgttcacc tctgcacgtt tcttgccgca
     4561 ggatacctgg tggcgggcgg gtgcggcgcg atcaactgct ggtatgaccg ggatatcgac
     4621 cgggtgatgg caaggacttc ggggagggtt atccccagga atgccctctc ccctcgccgg
     4681 gttctcctct ttggctctct cctgctgacg acaggactcc tggccgccgt actgatcctt
     4741 ccgttccttg cagccgccat gatggcggcg ggcgcaggca tctacctcat catctatacc
     4801 gcctggctga aacgggcaag ccacctgaac gtggtgttgg gcgggacggc ggtctgtttt
     4861 gccgcgtttg cggggtggac cgcgaccggc gcaccattcc ccacgctcac cccggtgctg
     4921 atcgcgctcg tggggttcct ctggactccg gggcatttct gggcgctcgc catataccgg
     4981 gaccaggatt accgtattgc ccgcatcccc atgctccccg ccagggcagg gcgatacccc
     5041 gcggcgcagg ccatcttcct ctggaacgcg gcaacagcgg gtctcaccgt cctcataccg
     5101 ttcctcacgg cgatcgaccg gatcactctc gccataacga tcccggccgg cctcttcctc
     5161 ctctccttga gcagaaggct tctcgtatcc ccatccgatg agagggcggt gcgcctcttc
     5221 tccttctcca tcgtgtacct gatgagcgtg ctcttcgcct tcctggtgct ggcggacggc
     5281 ctgccggcgc tgtaggaatc agggtaagcc ggcagcgcac ggtaatatct ctcactcttc
     5341 ctgcccgccg atcgcacccc ttgcgggcgg gacatctcta tggatgtccg gatcggtcga
     5401 acggaaggag aggatcgccg cgaccgcgta gattatcgcg ccgatgaacc acatggccgc
     5461 ccctcccatc tcctggtccc cgagcagcgt aacgccgggc aggtgggcgg tcgacgggta
     5521 caggtagacg atctggtcct ggaacgttat gatcgcgccg aggatcatcc cctgcatggc
     5581 gccggcaagg aggtacaacg ccttcccctc gtttgtcctg atgacgggaa ggtgcagccg
     5641 tgacccgggg acaagcggtg cccagtatgc gatgaacgca atgagcaggg tcacgtgctc
     5701 gaggatgtgc acgggcatat cggtgagcgt gatgtcgtac gggatgggta tgtgccagaa
     5761 ccacaggacg gccgtggaga ggataaacgc cgtgacgggg taactgatcg cgtagaggcc
     5821 gcggcgaagg gtccggtaca gggcccactt cctgagcgtt ccgggcggca gccaccacag
     5881 gaagaaatca gcggcaagga gagagggtgc aagtaccccg atcaggatca tatgatagac
     5941 catgtggatg gtgaaggact cccccagggc cggattgaga taggccatgg cgatgagcgt
     6001 cgccaccgcg gtaaaactgg cgaagatctt tgtggatcgt ccggtacggc cccgatcagc
     6061 ctttccattc ccctccatac cctacgaccc cccctccggc agtatatcta cgggaccccg
     6121 ggtgcgttcc caggcccggg gcacccggga cagcatgacg tagcctatcc agataacgcc
     6181 gacaccgatg agaatgatga aaggaaccgc gatcgaggcg aatatctgcc actcggggaa
     6241 gtgcacggcg taccgccggg ggatactgga tgccccggat gcaaggaaca taagggtaaa
     6301 cccggccccc ccgaccccgt atgccgccgc ggcataccgc gtgcgggggc tgctcccggc
     6361 tcccgagagg gtgcggagga tggagaagag gaacccccag aggaagacta ccgcacccag
     6421 gaggaaatag gtgtggaaat ggccgggaat ccagagggtg ttgtgcatca cctggttgac
     6481 ggcgatggtt gcatcgagcg ctgctgccat gcctccaaac atccaccccc agaggccgat
     6541 ggcgagaaag atggtggtta catcccaccg gatgcccgag cggtagtact gggagagccc
     6601 cccaaagagc gtgatcagga tcaccggtat caccgatacg taggttccta tctggcctgc
     6661 tacctgaaac gccaggggct gggcgaaatc ctggtacagg tggtggaggt agatgatggg
     6721 aacgatgaac gtgatggcgt tgagggcgag ggcgtaggta cggctcagtt tccaggcccg
     6781 gccggtgtag gcgggcagaa gaaagtacat cagcccggcg gcgaagtaga tggtcaggtt
     6841 caccaggaga tgcccgaaga gatagtcgaa gttcttcatg aagagcacat cgatgctctg
     6901 gatcagcccg gacgcctgca gccagagcgg gatgagccag gtaaagccgg ctatgacggc
     6961 gacgatcccg gggattgcga cggccaccgc gagcacatcc aggatggaca ggttctcctg
     7021 gcgagcagtc ctccggacga tgaggggcca ccccagcgcg ttgatgactc ccccgtgggc
     7081 gcgggagacc gtgcgggtga tcagcacaca gtagaacagg agcccgacga gcacgaaccc
     7141 tatgccgacc gtcgccccta tcacccccca gaaggcatac cccccatgaa acggcagagg
     7201 gtagagtgcg gtccaggcag ccgcaaaccc tcccccgagg gtgctgaaca ggacaaacat
     7261 catccctata aaaaagaaga agaaggtggc ccagagccag cgggtatcca ggaccaccct
     7321 ccgcccgatg acggcggcaa accccgcaat cgatcctaac agggcgaggg agaccatgga
     7381 gaggccatgc agggtcatga tccggtagaa gagttcgggc cccagcaggt tcaggagccc
     7441 cgactgctcc gtcctcatgg tgaggccgaa gatacccatg atgacgaaca tgacgaaggc
     7501 gaacaccatg ccggtcaggg taaagcggag cacgcctccg tagttggcgg gcagggaggg
     7561 tgtctttacg ggagggaccg catcactcga cattgaaggc tgcctccatt ccccagtggt
     7621 ccaccccgca gtactccagg cagtggatcc tgtacggccc cggccgggtg aaattgacga
     7681 tcaggcggtt ggtgtatccc ggcatggact gcgtttgagc gatgatcctc ccgtcggggt
     7741 cgaatatacc gaacccgtgg ttcacatcga tcgatgttac ggcaaactcc accgggacat
     7801 tggcggggat cgtggtgttg ctcaacgtga aggcgaactg cgacgtcgtt gcattgatga
     7861 cgaggacggg ttctcctacc gtgatgtccc tgaccggctg atacggaagg gcgacctggg
     7921 tgcccaggaa cacgacgacg gctgccagga ctatgacgga gtagagccgc cggcgaagga
     7981 gttccagccc ttctgtgata tcatcgtatc cgaccttctc tctgctgctc ctcatcaccc
     8041 atgcccagag gagcgtgtag gacccgccca tcacgagcag aaggacaaac acgtaccgcg
     8101 gatcgatgaa gatcatgtca ggccattacg gtcgggggtt gcacttaagg cttgtggcga
     8161 cgagccccga tcgccctcta tccccccgga acgggctgcc atgacgtatt ttccggcgga
     8221 gagcccctcg cgttctcaag cggaaagatc cttatggcag gcaggccgat catactaccg
     8281 gatgagaacg gttgtccgga ccaagacgag caacggcatc gattacctct acgagatcac
     8341 ctgctacgtt aaagcaactg cccgacaaac ctgatcggta cctatggctc ccgtgacaga
     8401 cggcgacacc ctgctcctgc acttcaccag cacccgccct gatggggaga tattcgagga
     8461 tacccgggcc ggcgagccgg tcagggcgac gcttggagag cgccagatca atcccctctt
     8521 tgaggaggcg ctgatcggga gggagccggg cgagacggtg accgtggtgc tgcccccgga
     8581 gaaggcctac gggaagtatc gcagccggct cgtgatcacg atgaagcgca agagactgaa
     8641 cctcgaccac gaaccggtgc ccggcgagtt cctgaaggtg gaagtgatgg gaaagccctg
     8701 cctggtgacc gtcaccgacg tgaccgaaaa gagcgtcacg atcgacgcca accacccgct
     8761 tgcaggggag acgatcacct acgcgataac ggtcgcggca atccttcccc gatccggtta
     8821 ggcccccgtg gtattactca acgggccacg gttacgcttc tttcccgggg aaagccctcg
     8881 cacccggcgg gttctgttcc atgacccggc aggatgcgcc ggccaaaacc cgtcgacaag
     8941 ctttatgacc ggtgccgtcg atgggcgccg ctgtccggag ttgccattat gaagatagtc
     9001 ggtatcaatg gaagcccccg gggcgggagg agcgccaccc tgcagctcat tgaggcggtc
     9061 cttgagggtg cgaaggagaa gggtgcagag gtcgatgtcg tgcaccttat cgaccttgac
     9121 cttaagttcc gcaacgcctg cagcgcctgt ttccaggagg gcaaatgcgt cgatgaagac
     9181 gacttcccca acctgtatga gaagatcaag gccgccgacg ggcttgtcct cggctcaccc
     9241 gtgtacttcg acctggtcac cggccagatg aagctgctca tcgaccggat ggccgacggg
     9301 atccagtgcc agtcgctcac ggggaagtac gggtgctcgg tcgccacgtc gggcgatcac
     9361 gccgaaccgg cggtcgtcgc gtacctgaat cacttcctcc agatgctcgg cgcaacgccg
     9421 gtcggcaacg tgggggtcgc gatcgggaag gacccgggtg ccctggcaaa ggcaaaagag
     9481 actgctcacg agcttggaaa gacgcttgcc gaagcgatcc agacccggcg gcagtacccg
     9541 cagatcgagg cgttccacaa gcggttccag gagaagttca aagcggtcat cgccggtgag
     9601 aagcccgagt ggccgggcga ttacgatcac tgggtcaacc aggcatgggt ctggtgagcc
     9661 tgcgcccgcc ggcgataccc ggtgagcccg ggcttttcga gtgcggctct cgtcgccccg
     9721 ggatccgggt ttttgagtcg ttcccggccc ccctctaccc ccgggtccag aacgcacgct
     9781 ccggcagcaa ccggccccaa cctatatata gcgtcccgct gcatgggcgc gggggaggcc
     9841 cgcccttccc tgcaggaggt catgtacgat gcccgagttt gcaaacccgt tctccgggaa
     9901 caggatggag cgcaggctgg accggggtga gctcatccgg acgatccggt tctcgatcgc
     9961 cgccgagtac gaggcggtcc agttctacga gcagatagcc gagtcgaccg acgatccgct
    10021 ggtccagaag gtgatgcgcg acgtcgcgaa tgaagagaag gagcatgcag gggagtttct
    10081 gcggcttctc cgggagatcg agcccaccga ggatggattt taccagcacg gctacaagga
    10141 agtcgaggag atgatcgcgg aagtgaagaa gggcggcagg tgatcccctt cctttgaggt
    10201 gccggtattt gcacttgaag ggtcgccgcc gaaccgcccc ggtgnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnggctcc cgggcgttct tcgctcgatt ggccccggga
    10381 tcgcgaggca ccgggccggc ccccttctgc cccgcgattc ccgggcagtg aacccgccgg
    10441 cgggcgagcc ggtcgttgag gatcacccgt tgaagatttc gggcccgggt ttaaggcacc
    10501 aaccagaata cagaaatacc ggcggcgccc cacattggag cattaacacc ctataacccc
    10561 gatcatcggg aggcttgcgc aatgaagaaa ggatttgtga cagatatcga gacagagacg
    10621 gtaaagaata cggatttccg cagggtcctc tacacgggga agttcagcca gctcgtgctg
    10681 atgtgcctca agcccggtga ggagatcggc gcagaggttc acgacgatgt cgaccagttc
    10741 ttccggttcg aggagggcga aggggccgtc gtgatcgacg gcgtgaagca cgccgtcagg
    10801 gatggcagcg cggtggttgt accgagcggt gcgaatcaca acgtcctgaa cacctctaag
    10861 actgccgacc tcaagctcta cacgatctac tccccgcccg agcaccagga taaggtcgtg
    10921 cgaaagaccc gcgaagaggc catggcccgg gaagagcatt ttgacgggaa gacgaccgag
    10981 taatctcccc tgaagcgtat aacgatccat ctcgcttttt ccggcggccg ctgtcccggt
    11041 ggttatcggg ccggccgcca tcgaaaaccc tcttcccctc atcccggtcg cgagcccggc
    11101 atagcccggg atatccgctg gcttcacctg cgtcccggcc ggccgtcacg cgcctccggc
    11161 tcgcggtctg gttcgctatc gggcctgcat tgcgtacggg agccggcgat gggggaagcg
    11221 aagtgatgca ccgggatacg ggagaagagc tgcggtagtg gcggcgaagg ccgggaagcc
    11281 ttcaggatcg tgggccgagg agcccccctt cttctcgtcg ggggtagttg agatgaggtc
    11341 acccaaggag cgtcgtaacc ttctgcacca gagccacggc tgcgctcact gcaaattctg
    11401 cctcggccgc agatatcgtg cctacggtgt cgtagaccga actgtgtctt ttacggcgca
    11461 tgcgatcaaa gacaagggcg tcaccctcac tgaggaagag tccggcaaac ttcaccaccg
    11521 atatgtgctg gtttgcgccg tccggacgat atcccttcgc aaacataaga gcccggccag
    11581 cctggagggc tgcgttgtag gcgatagtat acgcccagtc gcaatcggtc gaaagaagcg
    11641 tacgagcggt atcaatatcc cggcgagcaa gagccatcgc atcagccact tttttcgggt
    11701 cgggagggag cctctggatc agtccctgcc tctcaagatc ctcgatcatc gcaagatcca
    11761 atgatcatga tctttctctc gcccattaca ttctttacga aaggatctcc ggtctccctt
    11821 cgttgtgcaa actcgctgcc gcgcatgagc gtataattga tctcgcggtt gatcgcgcgt
    11881 tcgcctgtat gcacgagcgg gatcagcacc tcttcgttca catcgcccac gatgaagagg
    11941 tcgatatcgc tccttccccc tgcggtacct cttgcaaacg acccgtagat gaacatgcac
    12001 tcgatattct gcagggaaga gaggttctcc cggataaccc gtgcaacccc ttccgtcttg
    12061 agcacgagtt gctggaggtc ggtgtagagg aaaaagtcac ggttgaccgt gaagtagtgc
    12121 tggttccccc gccgtctccc gataagaagc ccaaacgact ccaggtttgc aagttcgcgg
    12181 cgcaccccat tgatgttctc gccggtcatg cggacgatct cgcggatgta aaactcgctc
    12241 tccgggttga ggagaaacag ggtgaggagt ttcacccggg cctttgatgg gatgagcgct
    12301 tcgagcatga atacatcatg tattcaaaag gataaataat gtattcatct gaatacaaaa
    12361 agtattcagt ctcacaacag atcctgccgg cgcaccctgg aaggcagggg ggcggccatg
    12421 ccgtcgaagg cggacgaggc agacgggaag aacctcgcac cacggggccg gcatacgaga
    12481 ccgggctccc gtggagcacc cctcaccgtt cctccgggct cgaatacgtc cgggccgggg
    12541 cctgcccggc gctgcatgcc gcctcgacgt ctgcaaggtc gtagacgagg aacccctcct
    12601 cgcggagccg gcctttcccc tcgacgccgc ccgcgacaag gcagaagcgc tcgtgggtgt
    12661 agcggtcgtg gcggatccgc tccgctttct gcacaaggcg cgtgagcacg ccgcgggctt
    12721 ctcgctccgt gagatctccc cacttgcatt ccccaaagac gatgctcgcg tcgctctccc
    12781 tggtggcgac gaggtcgacc tcatcgccgc ggtaccacca ccggccgata gtgtaccccg
    12841 gaaaggccgc ccgggcgagc tcgtttcgca tgaaaacctc gaactgtttg ccgaagtagg
    12901 ggttcgtatc gatggtatcg atcgggtgat tctccatctc gatcgcctgc cggtgagaga
    12961 agacgaagtt ataccagaag tcaaagatgc agtcctcgat gcggtacaac ccctgccgcc
    13021 ggctggcgag gaggggcagc tccctctcga tgatccggag gcgcgtcatc gcctcgaggt
    13081 aggggtagag gtggcgggca tccatgccgc aaaactgggc gatggtgccc ggggccgtgt
    13141 tgccgacggc gatggcattg aggatcgcct ggtaggtctt gagttcccga aactcctgtg
    13201 agaggaggaa gtagggctcc cggtagaagt agccgtacct gttgaagaac tcccgctcga
    13261 cgaaggtctc gtagcttctg tagtctccgg ccttgaggag gtattcgggg atcccgccga
    13321 tggtcaggta ggctttgaga gcatccccga aggagaacga agggaggaac tgccgtgcat
    13381 cgcggaaccg gagcggccca aggagcatat cgcgggtccg cctcccgtag aggggggagg
    13441 tgtaggagag cgcgctgtcg gtcatcaacc cgagcatcga cccggagagg aggatgcacc
    13501 agtttgaccc ggcaagggcg gtgtcccagg ctttctggag cgcggagagg atggtcgggt
    13561 cgttcttgat caggtagatg aattcgtcta taacgaggta gcggcgctcc gtgagcggta
    13621 tccgggtgat gtaggtgaag agctgatccc atgtcgtgat atcgagcgtt gcaaggagcg
    13681 ggtcggcgaa ggaccgggct atcgcctcct gaagccgccg gatctggatc tgcggcgcgg
    13741 tgtcctcggc gaaatggagg ataccctctt tcccgcggac gaattcggtg accagccggg
    13801 tctttcctat ccggcggcgc ccgtagagga tgatcagccg gccgccgtcc ttctgccact
    13861 cctcctccag gagggcacgc tcggtctcgc ggtcgacgaa ttccctaatc atgattagta
    13921 ctaatctgga ttagtattaa gggctccggt gccggggcga atcagcgggt caggcacgcc
    13981 ggcattcgtc tgacgacccc ggcgcgggat atcccgctgc acgggtccgg cgcagatacc
    14041 cttatgtgga caaattgtaa atgctattta caatatgtcc agtcccccac agaggatgat
    14101 ccggcgggat gcagaggcgc agatccggtc gctcgctctt ggctttccgg cagtggccgt
    14161 catcggcccc cgccagtcgg gaaagacgac gcttgtccgg tcggtattcc cccacctgcc
    14221 ctatgtttta ctcgaggacc ccgacacccg ggccttcgcg gaggaggacc cccgcagttt
    14281 tctcatacac tacgagaaga ccggggcaat cctcgatgag gtgcagaggg ttccggaact
    14341 cttctcgtac ctccagggag tccttgacgc gaaccagagg ccgggacagt tcatcctcac
    14401 ggggtcgcag aacttcctga tgatggagcg gatatcccag tcgctcgccg gtcgggtcgg
    14461 gatcgtcaaa ctcctccccc tctcgatggg agagcttgcc cgcgccggcc tcgaggctga
    14521 acgctacgag gacctcctct acgccggcct cttcccccgc ccctacagca gtgccgttca
    14581 cccgcgagac ttctattcat cctacatcca gacctacatc gagcgcgatc tccgccttct
    14641 caagcaggtg cagaacctct cggcgttcca gacgttcatg aagatgtgcg cctaccgatc
    14701 cggccaggtg gtaaactact catccctcgc acatgactgc ggcatcaccc acaacacggc
    14761 gaaagagtgg ttatcactcc tcgagacgtc gatgctgatc gtccaggtca ggccccaccg
    14821 caagaacttc aacaagcggc ttgtcaggat gccgaaactc tacttcacgg accccggtct
    14881 tgctgcgcac ctggccgggg tgcagggcgc cgacgatctc atctaccatc ccctgagggg
    14941 agggctcttt gaatcgctca tcatagcgga gttcctgaag ttccgcctca accggggtaa
    15001 ggagtccaac ctctacttct ggcgggacaa actcgggcac gagatcgact gtatcatcga
    15061 gtatatgggg tgggatccca tcccggtcga gatcaaatcg ggccgaacgc caagccccga
    15121 cttcttcaat gagatcgcct actggaacga actctccggc aacgccccgg atcgttcgtt
    15181 cgtggtctat gggggcgatc ggtcccagcg tcgggcggca ggacacctgg tcgggtatcg
    15241 agacctggaa ccaatcatcc ggtacctgga gtgaggggag gccggcggca tgcaccggat
    15301 tccaggagca tccagaccgg tgcccctgga gagcgcggta tactcctccc tcttacgctc
    15361 cccgcaccgc tcggcatgcg ccatcagacc actcccgtga cagagtcttt ccgctcctcc
    15421 ccccgcagca cccccgccgc atagcaggag agcgccatct ccacatccgc cgcggcccag
    15481 ataccctcgc tccggaggcc cgcggcggca aaccccgccg ggtgcgggca ggagacctca
    15541 tcgcggttga gaccgcccgc aaccgcctgc agggcccctg cccacgctcc gtactccccc
    15601 ggccaccccg gttgcccggc agggatcgcc caccgatccc cggaaggctc cacggcaagg
    15661 tcgagcaggg gatagcgctg gctcccggag tcgccgcggc caaagactct gatcagccgg
    15721 gcgtcgagcg tgccaaagac ctgcgggacg gcgaaccgga ggagtttgct cgcacatacc
    15781 ggcccgagcc cccggatctg tctctcgacg gtctggatgg tctcctccgg ctccagcatc
    15841 agccagtatg ccggggcgct gccgatgtag agggtgacgg cgatccgggc cggggccgag
    15901 agatcgtccc ggcgggcgat cttctgcacg tggtcgagcc cgagcgccag gctccgcgcg
    15961 gcggccgtca cctcccgctc aaggtcgagg atctccaaaa accccctctc ccaggtatcg
    16021 tcccgccacg tgtatccccg gtagagggcg ggaaagtcga gatcgtcgag cagccggccg
    16081 acctgctcct ccattacctc acctccggag agggtgcccc gccgatcctc tctgcccggc
    16141 ggggaaattt caggcggtag tcgaccgccg gcggccgcat caaccggtcg tcgatcttct
    16201 ccctccggta gcccttgacg tagccgtcga ttgcggccgt gaaccggacg atatcgccgc
    16261 ggcgaaggtc tcccactgcg tc
//
LOCUS       DAOSII010000037        16208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_3756_length_16208_cov_48.401659, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000037 DAOSII010000000
VERSION     DAOSII010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 16208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16208
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3756_length_16208_cov_48.401659"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="PLV90_04400"
     CDS             complement(<1..788)
                     /locus_tag="PLV90_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOF96387.1"
                     /translation="MDAFASLQRSIEKLVQIKCANYKEDYIKRRILSRMRLTNAADYE
                     AYHRYLLANPQEVNLLRNALTINVTKFFRDPEVFEVVRREVLPDLAGRGRSIRIWCAG
                     CSSGEEPYSIAILAHELTALRPDVSVMIYATDIDAVVLQKAMAGIYDAKALENVDERR
                     IRKHFVSHDDGTFEVRSHIKDLVKFRQHDLMSGIPITRYLDAVTCRNVTIYFTENQKN
                     ELARLFHSALGADGYYIMGKTEYLAREVEHLFSPYNIAQKILRKA"
     gene            complement(818..1297)
                     /locus_tag="PLV90_04405"
     CDS             complement(818..1297)
                     /locus_tag="PLV90_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOF96388.1"
                     /translation="MAASVDVVEFQLGEEHYALDIQIAREIVEMMPITPLPRAPRYLA
                     GIINLRGEITNIIDLRDLLGLPGSERVESRKIVVLMPDVAGGSNVGIIVDDVHSVVSV
                     RDDQIERINDGITSSISNYVKAIIKRGSGGDGKAKTLIIWLDVQKVIGDLRHYQSMP"
     gene            complement(1308..4172)
                     /locus_tag="PLV90_04410"
     CDS             complement(1308..4172)
                     /locus_tag="PLV90_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOF96389.1"
                     /translation="MMIDKPGRKALFTGPVVPGTTGQERGPVVLPGAIESALRSGIGG
                     DGSARIDLAGVPEEFRPLATSINALLAKSEEEKQDLRTRLEAAGALRQRSETIIQENP
                     MPILIVDPQFRITVANTAYADMSGIPKGQALGKSLRDFKITSQKGSGLRQAVQEKKRA
                     YAEVIVELPSGTHTLEQYGIPLLDEEGAIESILIVYNDITRQREGEEEIKSQMENIAE
                     LQRQSDTIIQKNPMPILVVDTAMKILTANDAYLQMTGIDRSRIASMTLRDFKVLAQKG
                     EGIRKVLTEKVRVRGEVTVEFPAGTYTLEQYGIPLLNDKNELTGILNVYNDITEKRKE
                     EEEIRRMQHRIETMIKQNPLAIATLRPDKSRIDINDEYARMWRGTREETLAKKLYDYD
                     ITILNGDHFYACYETKRLARTDVMVKWPDGVKKYLTLNAIPILDAKGDIEMAFYVWND
                     WTDLQGKEEEVKKTEHRVDAMIKQNPLAIATLRPDKSRIDINDEYARMWRGTREETLA
                     KKLYDYDITILNGDHFYACYETKRLARTDVMVKWPDGVKKYLTLNAIPILDAKGDIEM
                     AFYVWNDWTRSKEEEDQIRALMETAKQEGENLAKSAEGLGSALAAMAKGDLTAFVDAG
                     ADDPLQKVKSDYNASLTAIRSLLAEVARAAHQVELTTREVSKSSDEIIRATEQVAVST
                     QRSSEDARRQLDKIEEIGKDINDLSASIEEIASTSQEVMEHASKASKEGNEAASLGEV
                     ATNKMLLVEGISKQTVDEISTLNNQMREINNIVKLITDIANQTNLLALNAAIEAARAG
                     EHGRGFAVVAGEIRNLAGESKRASQDIEDLIRTIQASTEKTAGSMKASHQEIQAGIES
                     VNKVIGGLSRIVNGVEVVAHGITEITKATEDQASSTNNVMQKMDESTHMTKENLDRTE
                     DMAALAEEVSASTEEVGSAAHELAGMAEQLQKVMKQFKLN"
     gene            complement(4172..5053)
                     /locus_tag="PLV90_04415"
     CDS             complement(4172..5053)
                     /locus_tag="PLV90_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOF96390.1"
                     /translation="MLDITIVAFAHHKGGTGKTTSCLNVAGYLQKSGRSVLVVDCDPQ
                     ANATAGLGVDPDTLELSMYDLFMSAFEGFPDVVINDIIVPTASGVDLAPATLDLVGVE
                     PYLYSIEDRAGLLREALAGVRGNYDFILIDTPPSMGQFVINGLVAADHTIVTLDPGTF
                     ALRGMEALSTVFHDIREMLGENVTTDLAILTRWKGPDDRAAKTGGLALFLKRIFSPGP
                     SPEEERERERLKMFEAEVRKAFKQVFIVPYSPAIYETQQKGLPISHYAPESDAGREYR
                     AITTALLELDKNYGKDV"
     gene            complement(5185..5895)
                     /locus_tag="PLV90_04420"
     CDS             complement(5185..5895)
                     /locus_tag="PLV90_04420"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOF96391.1"
                     /translation="MDKADDDSRLHRIFRELQGKGLIGRERRLVQCPFTPSARGCPAL
                     NCIRYRRAFYERYLFGASDDTLRFVLLHEEGHIRSGSSLLSALPVLLALMLLPAALPF
                     LQAGGPLVRFASIALLAVTVFSIYRIYCRRMCDEEFAADRYAAEAMRCCYGVKDPGSL
                     LQNLLTGLRVEASAEPAPHRRTYGGLSRVIPGLFRSGPDYRPSIADRVQKIRAGVSAD
                     RLSSGRMRAPDTPVIPRE"
     gene            6223..6579
                     /locus_tag="PLV90_04425"
     CDS             6223..6579
                     /locus_tag="PLV90_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96392.1"
                     /translation="MSRYVGFLILALAALTVAAGCLSTGFGEVTYGGDALQVRVENTG
                     RPVENAVLQVTVTEVGALEQHEILSEARYVNLAPGENEYSVPVNLQPGTYRLFLTIFI
                     DGERKASVIRDLEVAP"
     gene            6576..8234
                     /gene="ade"
                     /locus_tag="PLV90_04430"
     CDS             6576..8234
                     /gene="ade"
                     /locus_tag="PLV90_04430"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HOF96393.1"
                     /translation="MTPAGLAAARGLEPADVVFSGGEVFNPFACAWERTDVAVSGGRI
                     VGLGDYEGKREYDLTGTYVVPGLIDAHVHIESSLLAPAEYARLVLRCGTTTVIADPHE
                     IANVCGAPGIEYMLAEGAQTPLDILVMLPSCVPATPFDLGGARLAAADLARFRGREGV
                     IGLAEVMNVPGVLAGDEDLRAKMDLFPVIDGHAPLLSGKDLNAYIYAGVQSDHECTAL
                     AEAREKLLRGMYVMIREGSTEQNLADLLPLVDACTAPRCCFATDDRHVDMLAGKGHID
                     DCIRRAVACGLEVELALRMATLSAAERFGLHDRGALAPGRLADFCVVDDPDRFRVLRT
                     FKRGIEVADAGYRPPACPAAPLHARVPAPRDIRIAGRGEARVIGIVPGQIVTRDLRCP
                     VDAADIPDTDRDILKAVVTDRYRGGGSGVGLVHGLGLREGALAGSVSHDSHNIVAVGV
                     ADADIVRAIGEVVRLGGGLTVVSGNEVTALPLECAGLMSALPYDEVARSLAALEEHAR
                     RLGAVENPFMYLSFLALTVIPATRVTERGVFDVTAFKDVPLFSE"
     gene            complement(8347..9426)
                     /locus_tag="PLV90_04435"
     CDS             complement(8347..9426)
                     /locus_tag="PLV90_04435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOF96394.1"
                     /translation="MTEVTDVAWILFVISIAALLALDLGVFNRKAHVIKPREALLQVA
                     VFIAAAVLFNIGIYTWMGGEAGLEFTTGYIMELMLSVDNLFVFVIVFASFCVPQEDQH
                     KVLFYGILGALLFRFAFIMAGVALVETFSWVLYIFGAFLIFTGIRMVTKKEEKAVEPD
                     KNILVRGFRKIMPVTGNYEGDRFFVRKTDAAGRAVLWATPMLVTLLVVETTDILFAVD
                     SIPAILGITTNPFIVFSSNAFAILGLRSMYFALAHIMSAFCYLKYGLAGILSFIGVKM
                     LVVEIYHVPVVVSLAIILVILGATIIASLIRNRRTGTCPEIVSVQADACPALRSLRAD
                     DAPAPMAETARSISKEIAEVKKGKE"
     gene            complement(9868..10617)
                     /locus_tag="PLV90_04440"
     CDS             complement(9868..10617)
                     /locus_tag="PLV90_04440"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HOF96395.1"
                     /translation="MQGERYWEIDAARGIAVVTMIVFHSAFDLNFFGVLPLPVSGGFL
                     RMLAYMTASTFIFIVGVSFTISYARASQRLAGRDLALKYARRGFTIFGYGLVITAVTW
                     LFLPSVYIVFGILHFIGAAILLAPLFARFSPASLILGSVACTIAGFITNAVPGPWPLL
                     WLGIHPASFASLDYVPLLPWFGIVLAGMAFGGLFYPDGRRKFALRTAEPAPARALSFL
                     GRHSLFIYFLHQPLILLLIAIVAPEAMPGLW"
     gene            complement(10629..10787)
                     /locus_tag="PLV90_04445"
     CDS             complement(10629..10787)
                     /locus_tag="PLV90_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96396.1"
                     /translation="MGRICSPFIVLECSRECGFSRIYNEPTGEQSAEIADTKVCPACG
                     APVRRRFF"
     gene            10911..11768
                     /locus_tag="PLV90_04450"
     CDS             10911..11768
                     /locus_tag="PLV90_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2099 family protein"
                     /protein_id="HOF96397.1"
                     /translation="MSDRDEHIVEAAGRCRVVIQNGRVVDVGTPQIDDCPLARRFACP
                     VDEMTPEAIRKNIEGRIRSFGMCTPQREVLAGPDFVIFGASELLSSAVRRGDLDAAVI
                     ASDGAGTLVATNPALIQGIGGRMSGLVKTSPLPAVIARIEENGGAVLDPATAVIDQAA
                     GVALAATLGHRRIAVTTASAAEAAVIRERFSDAVIVAVHTTGMSREDAVLMVRAADLV
                     TACASQHIRDAAAKTALLQAGRSIPVFAMTPAGKAIILGKVAETGQQVVVHGAHLPVS
                     GRESPSPLC"
     gene            complement(11773..12468)
                     /locus_tag="PLV90_04455"
     CDS             complement(11773..12468)
                     /locus_tag="PLV90_04455"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOF96398.1"
                     /translation="MQTTSGTRHNTVEAVICDMDNTLFDFVGAKREACRCVVDYIGAG
                     DPEALFSRFISGTYGFEDHRNIRGYLDDLGVYDPGTFEVCCRTYEDVKLDRIEVYPGV
                     EETLRHLADAGIRLAVATDAELVQARRRLGRTGLIDLFEVVVTPEISGKRKPEPDSLL
                     YALRRLDTAPAAAMMVGDSPVRDIAPGRRLGMVTAFAAYGDWHGNGPADAGADIVLAR
                     FSELADHLGIPGR"
     gene            complement(12432..13439)
                     /locus_tag="PLV90_04460"
     CDS             complement(12432..13439)
                     /locus_tag="PLV90_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96399.1"
                     /translation="MNDAIVREAELQVLLNSLDAIRVTYPLYKGDLLVAHPEGTGFRL
                     ELPADRETFQNWLAGYGPIAAELPSYADFSECMFASGISRYTNQAAFDAMRTSYGQLK
                     KTVFFGLDTNLFYHGFASNNPGIDHASYLIVDTVRDEIAYAINRKYPRKMIDEMIEHA
                     PGCREFIGELENKRMKRSRKAAYLALKEYRTIRDRATEIASPGPHTHLSEENDRNIVK
                     ALRKFEEERYALPVLLTADIYMADLCMTEGLEYFYFDRPYRLEATTCTAPAFRRLLFN
                     LAVVFGFIGCSGTTIFGEYGGKGNDLDELKVRFRDDEIYHEFSREVSICRQLLALGIT
                     R"
     gene            complement(13680..14195)
                     /locus_tag="PLV90_04465"
     CDS             complement(13680..14195)
                     /locus_tag="PLV90_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96400.1"
                     /translation="MISAPGDVYITLLGRSTWALVNAYQAVLREKGLRPERVAIVTEE
                     PYAANAAIAAGAVRIVSEGYGFVPTVDVEVLPEADFVQAGRTIRSLASDFIRQGCHVA
                     IDITSGRKVTVAGALIAVSLAELDIRHIYYLAMKSTDDVAKPYMMIPHQIQKIRDIME
                     DAGALSSTASG"
     gene            14515..15117
                     /locus_tag="PLV90_04470"
     CDS             14515..15117
                     /locus_tag="PLV90_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOF96401.1"
                     /translation="METRRGKLRTPAETWGLADPEQRRRTRQDAIDDGDLIEITRMGR
                     DIGITFPLAVSARAAQSMVPFPNIPQETVTENLWDTLHAFRDKALMMAAEEFEFQASI
                     YLNGLVPMLTFKAAVSPGDDGEPVITIMLPDEDWETIGCGRHSAGDRMLTVDDVASTL
                     NFTPGRIREFIREERIPAVKCGGSWRIKRSELERIMNEGF"
     gene            15199..>16208
                     /locus_tag="PLV90_04475"
     CDS             15199..>16208
                     /locus_tag="PLV90_04475"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOF96402.1"
                     /translation="MCSGASRELPNASLIDPVAGLDDGAAERTYCADGCAPGPGRDCG
                     RRSCILLSLFFCVALLALLFSTPADLWTPPAIEPLSPIVGLPGIFGPDARSFFKRWRG
                     RRAGEEEAPPDPAPVEHSGSEAGAPVKLEEAPANSLQLDDPVLPDSADAAGKTPEEFF
                     WFDDPLLPDGADALPVEQEPDDCSGEAGELPEPPPRSEPLTDAELMRLLGLDDEDDEP
                     SSGTPGYRAFPAEDEPTFAPGEDRPVESLIDDLGSPDADIRRRAVDAIREHGADAVAP
                     LVEALARADDGRRWCVAEALAAIGDDAIPALIAALGDDAVQTGAAATLVRIGEPAVPP
                     LIA"
BASE COUNT         2824 a         5070 c         5091 g         3223 t
ORIGIN      
        1 gccttgcgca gtatcttctg cgcaatgttg tagggtgaaa agagatgctc aacctctcgc
       61 gccaggtact ccgtcttccc catgatgtaa tagccatcag cgccgagtgc ggagtgaaag
      121 aggcgtgcaa gttcgttctt ctggttctcg gtgaagtaga tggtgacgtt tctgcaggtg
      181 acggcatcaa ggtaccgggt tatcgggatg cccgacatca ggtcgtgctg ccggaactta
      241 acgagatcct tgatgtggga gcggacctcg aacgtgccgt catcgtggct gacgaagtgc
      301 ttgcggatcc tcctctcatc cacgttctca agcgcctttg cgtcatatat gccggccatg
      361 gccttctgca gcaccacggc gtcgatgtcg gtcgcgtata tcatcacgct gacatccggg
      421 cgaagcgccg tcaattcgtg cgcgaggatg gcgatcgagt agggttcttc acccgaagag
      481 caaccggcac accagatccg gatcgatctc ccgcggccgg caagatccgg cagcacctcc
      541 cgccgcacca cctcgaatac ctcggggtcc cggaagaact tcgtgacgtt gatggtgaga
      601 gcgttcctga gcaggttgac ctcctgcggg tttgcaagga ggtacctgtg gtaggcctcg
      661 taatccgcgg cgttcgtcag gcgcatccgc gagaggatgc gccgcttgat gtagtcctcc
      721 ttgtagtttg cacacttgat ctggaccagt ttctcgatgc ttctctggag tgacgcaaat
      781 gcatccatgt gggggctctc ctcctgccgg ggatcgctta tggcatgctc tggtagtgcc
      841 tgagatcgcc gatcactttc tggacgtcga gccagatgat gagggtcttt gctttcccgt
      901 cgccgccgct ccccctcttg atgatcgcct tgacatagtt gctgattgac gacgtgatgc
      961 catcgttgat gcgctcgatc tgatcgtcgc gaaccgagac gacactgtgc acatcgtcga
     1021 cgatgatccc cacgttcgac ccgcccgcca cgtccggcat cagcacgacg atcttccggc
     1081 tctccacccg ttctgaaccg gggagcccga ggagatctct gagatcgatg atgttcgtga
     1141 tctcgccccg caggttgatg atgcccgcaa ggtaccgggg cgcacggggg aggggagtga
     1201 tcggcatcat ctccacgatc tctcgcgcta tctggatatc cagcgcataa tgctcttccc
     1261 cgagctggaa ttccacaacg tctacggatg ctgccatcgt ctccccctca gttcagcttg
     1321 aactgcttca tgaccttctg gagttgctcg gccatacctg ccagttcgtg cgctgcactc
     1381 ccgacctctt ctgtcgatgc gctgacctct tctgcaagcg ctgccatatc ctcggtgcga
     1441 tccaggttct ccttggtcat gtgggtggac tcgtccatct tctgcatgac gttgttcgtg
     1501 gaactggcct ggtcttcggt ggccttcgtg atctcggtga tgccgtgggc gacgacctcc
     1561 accccgttga cgatccggct caggcctccg atgaccttgt tgacgctctc gatgcctgcc
     1621 tggatctcct ggtgagatgc cttcatggag cctgcggtct tctcggtgct cgcctggatc
     1681 gtccggatca ggtcctcgat atcctggctc gcccgcttgg actcgccggc aaggttcctg
     1741 atctcgccgg caaccacggc gaacccgcgg ccgtgctccc cggcccgggc ggcctcgatc
     1801 gcggcgttga gcgcgaggag gttggtctgg tttgcaatat cggtgatcaa cttcacgatg
     1861 ttgttgatct cccgcatctg gttgttgagg gtgctgatct cgtccacggt ctgctttgag
     1921 atcccctcga cgaggagcat cttattcgtc gcgacctcgc cgagcgaggc ggcttcatta
     1981 ccctctttcg acgcctttga ggcgtgttcc atgacctctt gcgatgtact tgcaatctcc
     2041 tcgatggatg ctgagagatc gttgatatcc ttcccgatct cttcgatctt gtcgagctgg
     2101 cgccgcgcat cctcagacga ccgctgcgtc gagacggcga cctgctccgt ggcccggatg
     2161 atctcgtcag agctcttgct gacctctctt gttgtcagct cgacctgatg agccgcccgt
     2221 gccacctccg cgaggagcga gcggatggcg gtcagcgagg cgttgtaatc gctcttcacc
     2281 ttctggaggg ggtcgtccgc tcctgcatcc acaaacgcgg tcaggtcgcc cttcgccatc
     2341 gcagcgagtg cagaaccgag cccctcagcg ctttttgcaa gattctcgcc ctcctgtttc
     2401 gctgtctcca tcagggccct gatctggtct tcctcttcct tgctcctggt ccagtcgttc
     2461 cagacgtaga aggccatctc gatatcgcct ttcgcatcga ggatgggtat ggcgttcagg
     2521 gtgaggtatt tcttcacgcc gtcgggccac ttgaccatga cgtcggtccg tgcgagcctc
     2581 ttcgtctcgt agcaggcgta gaagtgatcg ccgttgagga tggtgatgtc gtagtcgtag
     2641 agtttctttg caagggtctc ctcgcgggtt ccccgccaca tccgggcata ctcgtcgttg
     2701 atgtcgatcc ggctcttatc gggacggagg gtcgcgatcg cgagcgggtt ctgcttgatc
     2761 atcgcatcca cgcggtgctc ggtcttcttg acctcctcct ccttcccctg cagatcggtc
     2821 cagtcgttcc agacgtagaa ggccatctcg atatcgcctt tcgcatcgag gatgggtatg
     2881 gcgttcaggg tgaggtattt cttcacgccg tcgggccact tgaccatgac gtcggtccgt
     2941 gcgagcctct tcgtctcgta gcaggcgtag aagtgatcgc cgttgaggat ggtgatgtcg
     3001 tagtcgtaga gtttctttgc aagggtctcc tcgcgggttc cccgccacat ccgggcatac
     3061 tcgtcgttga tgtcgatccg gctcttatcg ggacggaggg tcgcgatcgc gagcgggttc
     3121 tgcttgatca tcgtctcgat gcgatgctgc atcctccgga tctcttcctc ctcttttcgc
     3181 ttctcggtga tatcgttata gacattcagg atccccgtca gttcgttttt atcgttgagg
     3241 agcgggatcc cgtactgctc aagagtgtag gtgccggcgg ggaactcgac cgtcacttcg
     3301 ccccggaccc gtaccttctc ggtgagcact tttcggatcc cttctccttt ctgggcgaga
     3361 accttgaaat cccgcagcgt catcgaggcg atgcgcgacc ggtcgatgcc ggtcatctgg
     3421 aggtatgcat cgttggccgt gaggatcttc atcgcggtat cgacgaccag gatgggcatc
     3481 gggttcttct ggatgatcgt atcggactgt cgttgcaact cggcgatgtt ctccatctgg
     3541 ctcttgatct cttcctctcc ctcccgctgc cgggtgatgt cgttgtagac gatgaggatg
     3601 ctctcgatgg cgccctcctc atcgaggaga gggataccgt actgctcgag cgtatgggtg
     3661 cccgagggaa gttcgacgat gacctcggcg tatgcccgct tcttctcctg gaccgcctgc
     3721 cggaggcctg agcccttctg cgatgttatc ttgaaatctc tcaggctttt gccgagcgcc
     3781 tgacccttcg ggatcccgct catatcggca tacgcggtgt tggcgaccgt gatccggaac
     3841 tgcggatcga cgatcaggat gggcatcggg ttctcctgta tgatcgtctc ggatcgttgt
     3901 cggagggctc ccgcagcctc caggcgggtc cggaggtcct gtttctcctc ctcgctcttt
     3961 gcgagcagcg cgttgattga ggtagccagg ggccggaact cctccggaac gccggcaagg
     4021 tcgatcctcg cggaaccgtc gccgccgatc cccgagcgga gcgcagactc gatcgccccg
     4081 ggcagtacta ccggcccccg ttcctgtcct gtcgtaccgg ggacaaccgg cccggtaaat
     4141 agtgctttcc ttccaggttt atcgatcatc attacacgtc ctttccatag ttcttatcca
     4201 gttccagaag cgccgtggtt attgcccggt attccctgcc ggcatcgctc tcgggcgcgt
     4261 agtgggagat tggcaggcct ttttgctgtg tctcgtagat tgcaggcgag taagggacta
     4321 tgaacacctg tttgaacgct tttctgacct cggcctcaaa catcttcaac cgctctctct
     4381 ccctctcctc ctcgggggag gggccggggg agaagatccg tttcaggaag agggcaaggc
     4441 cgcccgtttt tgccgcccgg tcgtccggcc ccttccagcg ggtcaggatg gccaagtcgg
     4501 tggtgacgtt ctctccaagc atctccctga tgtcgtgaaa gaccgtcgat agggcttcca
     4561 tacccctcag agcaaatgtg ccggggtcga gggtgacgat ggtgtggtcg gcagcgacga
     4621 ggccgttgat gacgaactgg cccatgctcg ggggcgtatc gatcaggatg aagtcgtagt
     4681 ttcccctcac cccggcgagc gcctccctga gcagtcctgc cctgtcctcg atgctgtaga
     4741 ggtagggctc aacccccacg aggtccaggg ttgcaggggc gagatcgact ccggacgccg
     4801 tggggacgat gatatcgttg atcacgacgt cgggaaaacc ctcaaacgcg ctcatgaaga
     4861 gatcgtacat gctcagttca agggtatcgg ggtcgacccc gagccctgcg gtggcgttgg
     4921 cctgcgggtc gcagtcgacc accagaacgc ttcttccact cttctggagg tagcctgcaa
     4981 cgttaaggca cgacgtcgtc tttcccgtgc cacctttgtg gtgggcgaac gctacaattg
     5041 tgatatccag cacccctcat tactttcttg gtaatatgta ttattaaacc tatttctata
     5101 ctacagcgaa tataagagca agacgctgca tttaaagcga tcaacaattc ccttatagga
     5161 aaaccagccc ggatatttcg attgctactc ccgcgggatg acgggggtat cgggcgcccg
     5221 catgcggccg gaggacaggc ggtcagcgga aaccccggcc cggatttttt gtacgcgatc
     5281 ggcgatggac ggccggtagt cgggccctga gcggaagagt ccggggatca cccgcgaaag
     5341 gccgccgtac gttctgcggt gcggggccgg ctcggccgat gcctcaaccc ggagcccggt
     5401 gaggaggttc tggaggagag agccggggtc cttgaccccg tagcagcatc tcatcgcttc
     5461 tgcggcatac cggtcggccg cgaactcctc gtcgcacatg cggcggcagt atatccggta
     5521 gatggagaag acggtcacgg cgaggagagc gatggaggcg aacctgacga gaggaccccc
     5581 tgcctgcagg aaaggcaggg ccgccggcag cagcatgagg gcgaggagga ccgggagggc
     5641 ggagaggaga gaagagccac tcctgatatg cccttcttcg tggagcagca cgaaccgcag
     5701 ggtatcgtcg cttgccccaa agaggtatct ttcgtagaag gcacgtcggt agcggatgca
     5761 gttgagcgcc gggcagcctc gcgcagaagg cgtgaagggg cactgcacca gccttctctc
     5821 gcggccgatc agccccttcc cctggagctc gcggaatatc cggtggaggc gggagtcatc
     5881 gtcggcctta tccattcaag atacattata ttttgtacac tatattaaat gatttcacat
     5941 cccgcagaac cgctcatcat ccgcctcgcc tcttttccgg gctatcgagc gctcaaggca
     6001 gccggggcca cgccgtcgcg cccgaccggt ggggatgccc ccgactccgg cccggagcgg
     6061 caccggatcg ggaacctgtg caatgtccgt ttttttcata atgttttaat aatagcaacc
     6121 aaaatggtga atgagtagag tggaggtgaa tttgcagcac cgggggtctg tacggcccgc
     6181 accgcgtggg cgcggcgtct gtccccccgg ttctccgagc gaatgtccag atacgtgggt
     6241 ttcctgatac ttgcccttgc cgccctcacc gtggctgccg ggtgcctctc cacaggattc
     6301 ggggaggtga cctacggcgg cgacgccctg caggtccgcg tggagaacac cggcaggccc
     6361 gtcgagaatg ccgtgctcca ggtgacggtc actgaggttg gcgcgcttga acagcacgag
     6421 atcctctctg aagcacggta tgtcaacctt gccccgggag agaacgagta ctcggtcccg
     6481 gtcaacctgc agcccggtac ttacaggctc tttttgacca tattcatcga cggcgagcgg
     6541 aaagcatccg tcatccgtga cctggaggtc gcaccctgac accggcaggc cttgccgcgg
     6601 cgcgggggct tgagccggca gacgtcgtct tctccggcgg cgaggtcttc aatcccttcg
     6661 cgtgcgcctg ggaacgcacg gacgtcgcgg tcagcggcgg ccgcatcgtc ggtctcggcg
     6721 actacgaggg gaagcgggag tacgacctca cgggaaccta tgttgttccc ggcctgatcg
     6781 atgcccacgt ccacatcgag agttctctcc tcgcgccggc ggagtatgcc cgactcgtcc
     6841 ttcgatgcgg cacgacgacc gtgatcgccg acccccacga gatagcgaac gtctgcgggg
     6901 cgccggggat cgagtacatg ctcgccgaag gggcgcagac accgctcgac attctggtga
     6961 tgctcccctc atgcgtcccg gcaacgccct tcgacctggg aggcgcccgg cttgccgccg
     7021 cagacctcgc ccggttccgg gggcgggaag gggtcatcgg gcttgccgag gtgatgaacg
     7081 tccccggcgt ccttgccggg gatgaggacc tccgggcaaa gatggatctc tttcccgtca
     7141 tcgacggcca cgcgccgctc ctctccggga aggacttaaa cgcctacatc tacgccggcg
     7201 tccagagcga ccacgagtgc acggcgctcg cggaggcgcg ggagaaactc ctcaggggga
     7261 tgtacgtcat gatccgggag ggctcgacgg agcagaacct cgccgatctc ctgccgctcg
     7321 tggatgcctg cacggcgccg cgatgctgct ttgccaccga cgaccggcac gtcgatatgc
     7381 ttgccggaaa ggggcatatc gacgactgta tccggagagc cgtcgcgtgc gggcttgagg
     7441 tcgagcttgc gctccgcatg gcgacgctct ccgccgccga gcggttcggg ctccacgacc
     7501 ggggcgctct tgctcccggg aggcttgccg acttctgcgt cgtcgacgac cccgaccggt
     7561 tccgcgtcct gcggacgttc aagcgcggca tcgaggtggc cgatgccggc taccgcccgc
     7621 cggcctgccc tgcggccccc ctgcatgccc gcgtgccggc cccgcgcgat atccggatcg
     7681 ccggccgggg cgaggcgcgg gtgatcggta tcgtgcccgg ccagatcgtg acccgtgacc
     7741 tgcgctgccc ggtggatgcc gccgatatcc cggacaccga ccgcgacatc ctcaaagcgg
     7801 tcgtcaccga ccgctaccgg gggggaggct ccggcgtcgg gctcgtccac ggtctcggtc
     7861 tccgggaagg ggcgcttgcc ggctcggtct cccacgactc ccacaacatc gtcgctgtcg
     7921 gggttgccga cgccgatatc gtccgcgcca tcggcgaggt ggtccggctc gggggcgggc
     7981 ttacggtggt atccggcaac gaggtgaccg cgctccccct cgagtgcgcc gggctgatgt
     8041 cggcccttcc ctacgatgag gtcgcgcgaa gcctcgcggc cctcgaagag cacgcccggc
     8101 ggctcggcgc ggtcgagaac cccttcatgt acctctcgtt cctcgccctg accgtcatcc
     8161 ccgccacccg ggtcacggag cggggggtct ttgacgttac ggcgtttaag gacgtgccgc
     8221 tgttctcgga gtgagagagg gcaggagatc gcctggcggt gcttgctctc tccccgatcg
     8281 cccctttcga gttagatccg cttcgtgccc ggcgcgaaaa aagggggaga ccaccccggc
     8341 ggcgggtcac tccttgcctt tcttcacttc tgcgatctct tttgatatgg aacgggcagt
     8401 ctctgccatg ggagccgggg cgtcgtctgc gcggaggctc cggagggccg ggcaggcatc
     8461 ggcctgcacg ctcactatct ccggacaggt tccggtgcgc cggttcctga taagggatgc
     8521 gatgatggtc gcccccagga tcacgaggat gatagcgagc gataccacca ccgggacatg
     8581 gtagatctcg acgaccagca tcttgacgcc gatgaaactg aggatgccgg cgagcccgta
     8641 cttcaggtag cagaacgcgc tcatgatgtg cgccagcgcg aagtacatcg acctcaggcc
     8701 gaggatggcg aaggcgttgg agctgaagac gatgaacgga ttggtggtga tgccgaggat
     8761 ggcggggatg gagtccaccg cgaagaggat gtcggtggtc tccaccacca gcagggtcac
     8821 gagcatgggg gtggcccaga gcaccgccct gccggccgca tcagtcttcc tgacgaagaa
     8881 cctgtcccct tcgtagttcc cggtgaccgg catgatcttc cggaacccgc ggaccaggat
     8941 gttcttgtcc ggttcgaccg ctttctcctc ctttttggtc accatcctga taccggtgaa
     9001 gatcaggaag gcgccgaaga tgtagagcac ccaggagaac gtctccacca gcgcgacacc
     9061 cgccatgatg aacgcaaaac ggaacagcag ggctcccagg atgccgtaga acagcacctt
     9121 gtgctggtcc tcctgcggca cgcagaacga ggcgaagacg atgacgaaga cgaagagatt
     9181 gtcgacgctc agcatcagct ccatgatgta gccggtggtg aactccagcc cggcctcccc
     9241 ccccatccag gtgtagatac cgatattaaa gagtactgcg gcggcgatga agacggcgac
     9301 ctgcaggagc gcttccctcg gctttatgac gtgggcttta cggttgaaga cgccgaggtc
     9361 taacgccagg agcgccgcaa tactgatgac gaacaggatc cacgctacgt ctgtcacttc
     9421 tgtcatagat tcacccggct cgatctgtca tatttcgggc tttccgggca cacattaccc
     9481 gggggccctc cgggcatcgt ctcgaccgta tcgggatgcc ggtacgcggg gcggaagaag
     9541 aactcaggat aggttttact ccggacgacg tcatcggtgt ttgggccgca cctgccgcaa
     9601 ccggcatgcc cctctccaga ataccctcaa gacagctctg catatgcgga tcggatcgcc
     9661 gggacgcccc ctccccgaca gaagcgcgtt catcggggtc cggtccggca acgtcccggc
     9721 tctttaataa ccggtcaaac ccggcctctc cccagatcat ttcagttccc ccggcaaata
     9781 ctttctgccg gcggtaaaac aacctcctgt cagggagcgg agaggctccg ctgtacccct
     9841 gtgatgcacg tgcggtccgc aaacccctca ccacagcccc ggcatcgcct ccggcgcgac
     9901 gatcgcgatc aggaggagta tgagaggctg gtgcaggaag tagatgaaga gcgagtgccg
     9961 gccgaggaac gagagcgccc gggccggtgc cggctctgcc gtccggagcg caaactttcg
    10021 ccgcccgtcc gggtaaaaaa gccccccgaa ggccatgccg gcgaggacga tgccgaacca
    10081 ggggaggagc ggcacgtaat cgaggcttgc gaaggacgcc gggtggatgc cgagccagag
    10141 gaggggccat gggcccggga cggcgtttgt gatgaagccg gctatggtgc aggcgacgct
    10201 cccgagtatg agggacgctg ggctgaaccg cgcaaagagc ggcgcgagca ggatcgcggc
    10261 cccgatgaag tgcaggatgc cgaagacgat gtagacggag gggaggaaga gccaggtgac
    10321 ggcggtgatg acgaggccgt agccgaatat cgtgaatccg cgccgggcgt acttgagcgc
    10381 aaggtcgcgg ccggcgaggc gctgcgaagc tctggcatag ctgatggtga aggagacccc
    10441 gacgatgaag atgaacgtcg acgcggtcat gtaggcgagc atgcggagga acccgccgga
    10501 gacggggagc ggcaggacgc cgaagaagtt gaggtcgaag gcggagtgaa agacgatcat
    10561 cgtcacgacg gcgatgcccc gggcggcgtc gatctcccag tagcgctctc cctgcacggt
    10621 ttctgccgtc agaagaacct tctccgcacg ggggcgccgc aggcaggaca gaccttcgta
    10681 tcggcaatct cggcgctctg ctccccggtg ggttcgttgt agatccggga gaaaccgcac
    10741 tctctgctgc actcgagcac gatgaacggg ctacagatcc ggcccatgcc tcctcacccc
    10801 gtcttggcgc accatggtct acattctcat ggagagatca ccggataagt agttttaccc
    10861 gctggcccgg gagggagagc atttttcccc atccacgccc taccggaggt atgagcgacc
    10921 gtgacgaaca tatcgtagag gccgccggca ggtgccgggt cgtcatccag aacggccgtg
    10981 tggtcgatgt cgggacgccg cagatagacg actgcccgct ggcacggcgg ttcgcctgcc
    11041 cggtcgatga gatgacgccg gaggcgatac ggaagaacat cgaagggcgc atccggtcct
    11101 tcggtatgtg tacgccacag cgcgaagtcc tcgccgggcc ggacttcgtc atcttcgggg
    11161 cctccgaact cttaagcagc gccgtccgga ggggagacct cgacgccgcg gtgatcgcct
    11221 ccgatggggc cgggacgctg gtcgcgacga accccgccct catccagggt atcggcggcc
    11281 ggatgtcggg cctcgtgaag acgagccccc tcccagcggt gatcgcgcgg atcgaggaga
    11341 acggcggtgc ggtcctcgac ccggcaaccg ccgtcatcga tcaggccgcc ggcgtcgccc
    11401 tggcggcaac gctcggccac cgccggatag ccgtgaccac cgcctccgcg gccgaggcgg
    11461 cggtcatccg ggagcggttc tcggatgccg tgatcgtcgc cgtccacacg accggcatgt
    11521 cgcgggaaga cgcggtcctg atggtccgcg cggccgatct ggtcaccgcc tgcgcttcgc
    11581 agcatatccg ggatgctgcg gcaaagaccg cgctcctgca ggcaggaagg tcgataccgg
    11641 tctttgcgat gaccccggcg ggaaaggcga tcatactcgg gaaggtcgcc gagaccggcc
    11701 agcaggtcgt cgtccatggc gcgcacctcc cggtatcggg tcgggagtcc ccctcgccgc
    11761 tctgctgacg cttcaccggc ccgggatgcc gaggtgatcc gcgagctcgg agaagcgcgc
    11821 aagcacgata tcggccccgg catccgccgg gccgttcccg tgccagtccc cgtaggcggc
    11881 aaacgcggtc accataccaa gccgtctccc cggggcgatg tcgcgaaccg ggctatcgcc
    11941 caccatcatc gccgctgccg gcgccgtatc gagccgccgg agggcataga ggagggagtc
    12001 gggctcaggc ttgcgtttcc ccgatatctc cggggtcacc acgacctcga agagatcgat
    12061 aagcccggtc ctcccgagcc tccggcgcgc ctggacgagc tctgcgtcgg tggcgaccgc
    12121 gagcctgatg ccggcatccg cgaggtgccg gagcgtctcc tcgacgcccg ggtagacctc
    12181 gatcctatcg agtttcacgt cctcgtaggt gcggcagcag acctcgaacg tccccggatc
    12241 gtacaccccg aggtcgtcaa ggtagccgcg tatgttcctg tggtcctcaa acccgtaggt
    12301 gccgctgata aaccgcgaga agagcgcttc agggtctcct gccccgatgt agtcgaccac
    12361 gcaccggcac gcctcccgct ttgctccgac gaagtcaaag agggtgttgt ccatatcgca
    12421 gatgaccgcc tctaccgtgt tatgccgagt gccagaagtt gtctgcatat cgagacctct
    12481 cgtgagaatt catggtagat ttcgtcgtcc cggaaacgca ccttcagttc gtcgagatcg
    12541 ttgcccttcc cgccgtactc cccgaagatc gtggtgccgc tgcagcctat gaacccgaag
    12601 accacggcga ggttgaagag cagccgccgg aacgccggcg ccgtgcaggt tgttgcctca
    12661 aggcggtatg ggcggtcgaa gtagaaatac tcaagccctt ccgtcatgca gaggtccgcc
    12721 atgtagatgt cggcggtcag gagaaccgga agggcatacc gctcctcctc gaacttccgc
    12781 agcgccttga cgatgttacg gtcgttctcc tctgagaggt gcgtgtgcgg gcccggcgag
    12841 gctatctccg tcgcgcggtc gcggatggtg cggtactctt tcagggcgag gtatgccgct
    12901 ttccgggacc gtttcatccg cttgttctca agctccccga tgaactcccg gcagccgggc
    12961 gcatgctcta tcatctcatc gatcatcttt cgcgggtact tgcggttgat ggcatacgcg
    13021 atctcgtcgc ggacggtatc gacgatcagg taggaggcat gatcgatccc ggggttgttc
    13081 gatgcgaaac cgtggtagaa gaggtttgta tccaggccga agaagaccgt cttcttcagc
    13141 tgcccgtacg aggtgcgcat cgcatcaaag gcggcctggt ttgtgtaccg gctgatcccg
    13201 cttgcaaaca tgcactccga gaagtcggcg taagaaggga gctcggcggc gatcggcccg
    13261 tatccggcga gccagttctg gaaggtctcc ctatcggcgg ggagttcaag tcgaaaaccg
    13321 gtgccctccg ggtgcgcgac gaggaggtct cctttgtaga gcgggtacgt gacccggata
    13381 gcatcgaggc tgttgaggag gacctgcaac tcggcctccc tgacgatcgc gtcgttcacg
    13441 gtatcacggc gcgggtgcct ccggtaaata tcggaggagg aagcgcgccc tccttaccag
    13501 cccaaaggtg atcccgggta caacgcatcg gcgctcccgc gggcgaggca tgcacggcgg
    13561 gcaagcccgt cgccggataa ccggcaccgg ccggccggtg cgaataaggc cgcctgtaca
    13621 cattccatgg aaacgtctcc ggcgcactca aaataccttt ccctgcagcc acccggggat
    13681 caaccggagg cggtcgacga gagcgccccc gcatcctcca tgatatcacg gatcttctgg
    13741 atctggtgcg ggatcatcat atacggcttt gcgacgtcgt cggtgctctt catggcaagg
    13801 tagtagatgt gccggatgtc aagctccgca agcgagaccg ctatgagcgc cccggcgacc
    13861 gtgaccttcc ttcccgaggt gatatcgatc gccacgtggc atccctgccg gatgaagtcc
    13921 gaggcaagtg aacggatcgt ccggccggcc tgcacgaagt ccgcttcggg cagaacctcc
    13981 acatcgaccg tcggaacaaa cccgtatccc tccgagacta tacggactgc cccggccgcg
    14041 atcgctgcgt tcgccgcata aggttcttcg gtgacgatag cgacccgttc ggggcgcagt
    14101 cccttctccc gcaggactgc ctggtatgcg ttgacgagcg cccatgtcga gcggccgagg
    14161 agtgttatgt agacatcgcc gggggcggag atcatcgggg gatcaccgga gtagactcac
    14221 cccgcttgct atataggctt tgtcgggagc cccctttcgg gatgaggtgg tgcggtgggc
    14281 attccaggcc gcacgcttcg tcctttttgc ctccttcccc gtatatccgc ctctcccggg
    14341 gccatgcccg gtacatcgcc tgatttttgc ggcattccgc tcccgggatg ctcccaatac
    14401 gcctttaacg ctaaaggaat tgcaaaatct cgcaaataaa ttggtaaatt ttttatgacg
    14461 ggttccctca tagtggtatg gtagagcgcg aggatatccc ggagaatacg atccatggag
    14521 acgagaagag ggaagttgcg gacgccggca gagacctggg gattggcgga cccggagcaa
    14581 cggaggagga cacgccagga tgctatcgat gacggggatc tgatcgagat cacccggatg
    14641 ggcagggata tcggcatcac cttccccctc gcagtctcgg cacgggctgc gcagagcatg
    14701 gtcccgttcc cgaacatccc gcaggagacc gtgacggaga acctgtggga caccctccat
    14761 gcgttcaggg ataaagccct tatgatggcc gcagaggagt ttgagttcca ggcgagcatc
    14821 tacctcaacg gcctcgtccc catgctcacc ttcaaagccg cggtctcccc cggggatgac
    14881 ggcgaaccgg tcatcaccat catgctgccg gatgaggact gggagacgat cgggtgcggc
    14941 cgccacagcg cgggcgaccg gatgctcacc gtcgacgacg tcgcctcgac cctgaacttc
    15001 acccccggcc ggatccgtga gttcatccgc gaagagcgga tcccggcggt caagtgcggg
    15061 ggatcgtggc ggatcaagcg gtcggaactc gagcggatca tgaacgaggg tttctgatcc
    15121 cggccttact tatgagtttt ctcacgggcc ttgaactgga aacaaaccta tttaattttt
    15181 agacgtagag ttctccttat gtgctccggg gcctcacgag aactcccgaa cgcctctctc
    15241 atcgaccctg tcgccggact cgacgatggt gcggcagaac ggacatactg tgcggacggg
    15301 tgcgcgcccg ggcccgggcg ggactgcggg cggcggtcgt gcatcttgct gtccctgttc
    15361 ttctgcgttg cccttctcgc tctcctcttc tccacgccgg cagacctctg gacacctccg
    15421 gcgatagaac ccctctcgcc catcgtggga ttgcccggta tattcggtcc ggatgcgcgc
    15481 tcgttcttca aacgctggag agggcggcga gccggggagg aagaggcgcc tccggatccg
    15541 gccccggtcg agcactcggg gagcgaggcc ggcgcacccg tgaagcttga agaggcccct
    15601 gcgaactccc tgcagctcga cgaccccgtc ctcccggaca gcgcggatgc cgccgggaag
    15661 accccggagg agttcttctg gtttgacgat cccctcttgc ctgacggggc cgatgcgctg
    15721 cccgtcgagc aggagccgga cgattgctcc ggcgaggcgg gggaactccc ggagcctcct
    15781 ccccgctccg aacctctcac cgatgcggaa ctcatgaggc tgctcgggct cgacgatgaa
    15841 gacgatgaac cctcgtccgg gactccgggt taccgcgcct tcccggcgga ggatgaaccg
    15901 acctttgcgc ccggcgaaga tcgccccgtg gagtcgctca tcgacgacct cggcagcccc
    15961 gatgccgata tccgcaggcg tgccgtcgac gccatcagag agcacggggc cgatgccgtg
    16021 gctcccctgg tagaggccct cgctcgcgcg gacgacgggc ggcggtggtg cgtcgcagaa
    16081 gccctcgccg cgatcggtga cgacgcaatc ccggcgctga tcgcggctct cggtgacgat
    16141 gcggtgcaga ccggtgctgc cgccacgctc gtgcggatag gggagcccgc cgtgccgccc
    16201 ctcatcgc
//
LOCUS       DAOSII010000038        16005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_3822_length_16005_cov_48.429655, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000038 DAOSII010000000
VERSION     DAOSII010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 16005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16005
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3822_length_16005_cov_48.429655"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..1015
                     /locus_tag="PLV90_04480"
     CDS             194..1015
                     /locus_tag="PLV90_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96403.1"
                     /translation="MVGVDQAQIDKILAGASSDDPEARLDTLEKELDFVKTSIKRLLI
                     DLRERMNELDNPFTCAAPFGGSRLPPPTLPAGIEDDDLPGLDMLPPGLPTKKDDLSFP
                     GLPTKKDDLPFPGLQKKTDDLSFAAGLGDAGLFPPDIKPAPPDAKSPGKLKLQKVHRL
                     FAWVHQGCSKYGHEHMTIMIDAYRSMGYITDEVADQIREIMRMAPETRKDIADIGPNE
                     FVSELYVLNRILDPEDATMDRDLIEVLMLARRRQGKPDAPSRDADDAWIELLDRI"
     gene            1019..1522
                     /locus_tag="PLV90_04485"
     CDS             1019..1522
                     /locus_tag="PLV90_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOF96404.1"
                     /translation="MSSETFTTAMFLIAAIISAGVLINAVFPVVYTLAGTIASSSHKV
                     DERLSTDARIVTTYASEDNRIARIWLKNVGTGRIADADVRKADVFLGGQGDFIRLTWK
                     SGEPLAYGTWTHRILEETQNNYWDPGETLYIEGMAAEVPAKGQVVYFQFVLPTGLSRS
                     TTFTASD"
     gene            1525..1974
                     /locus_tag="PLV90_04490"
     CDS             1525..1974
                     /locus_tag="PLV90_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaF"
                     /protein_id="HOF96405.1"
                     /translation="MSAGPLIASGIGVILLVATAYVLVGGTLATTEVMVEAQNSLVAQ
                     QEIRMRTAITIQDAAIEGQTLYVDLKNTGSEPIVDVAAIDVYIYRDGTPHYTGDWTVV
                     DISPDVIHPGMLDPGETLFLAVRFEGNSPGYVQVVTGNGVSSSAYVR"
     gene            1981..2712
                     /locus_tag="PLV90_04495"
     CDS             1981..2712
                     /locus_tag="PLV90_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HOF96406.1"
                     /translation="MATDSSIPGLPDRSILSTGNSELDKKIADGLPLQSLTLIEGEND
                     TGKSVLTQQIIWGALKQGFNVDLFSTENTSKSFLSQMESMSLDISDYFAWGYLRVFPM
                     HVVGFEWTKEKMQGTLERMIAYMEQSKAQVIVIDSLTLFTEYAKQDTVLTFFTNCKNL
                     VDHGKTILITLHTYAFVEDSLVRIRSICDAHLFMKKALVGGKYVMMLEVVKVRGARKT
                     TGNIISFEVHPGYGIKIIPVSVAKV"
     gene            2718..4571
                     /locus_tag="PLV90_04500"
     CDS             2718..4571
                     /locus_tag="PLV90_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HOF96407.1"
                     /translation="MSSALEAMITLPFEPEFIDEGNDCYNNVESCVLYRMLPANAKDY
                     VRASPHLLEYLHILPVNTVGIPLFLSELKRDLKSMENPNIIYPVNETTFVHIFSDPND
                     VRNWYIPIEPSFLHSVKEIVPVIEAKLIDMIDALDEEPVTEEARIEVLKNFIRQLVYV
                     RQPGEEVDPSLLAGAAAKDIKSRIKTFLVSDIGASATRQAHGHPTLADGRLILTAQEY
                     KALEYLMVRDKIEMGTLKPFLSDKYIEDISCNGVGPIFIEHKIFKGLKSVVGFKDSGE
                     LDNFVIKLAERIKRPLTYRSPIVDATLPDGSRINIVYGTEISKHGSNFTIRKVNEVPL
                     SILQVIESGACDYMMAAYLWICLEYGMSMFVSGETASGKTTTLNALTTFLPPENKIVT
                     IEDTPELTVPHRNWTREVAKAKGKGEGEGSEITMFDLLKAALRQRPNQILVGEIRGVE
                     GSVAFSAMQTGHPVMSTFHAASVEKLIQRLCGDPINIPKTYVDNLNLVIIQSAVKLPR
                     GGTARRMLSINELVGYDPETQGFSFMAAFIWDPATDMFTFTGRGSSYLLENKIATMLG
                     IPENRRAEIYDEIDKRAKILERLHKAGYTQFWDLFHMTTKIKKQGLLTIEV"
     gene            4573..6171
                     /gene="flaJ"
                     /locus_tag="PLV90_04505"
     CDS             4573..6171
                     /gene="flaJ"
                     /locus_tag="PLV90_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaellar assembly protein FlaJ"
                     /protein_id="HOF96408.1"
                     /translation="MFEDVAERLRAANQGKIPFEEEVGRLTDLRSSILENKKMEQDLL
                     LMYTYMAAITTSAVTRPEIFQYTSERIEYIPSRYIAQVQRLVAGWGHSYAGALRAVAE
                     RCRNDTLQSMLNRYANSIDSGVPDDAFIETELSSIRSIYRNSFEQGLELLKKWGDAYI
                     AMLLSGSLVAIIIMISVAIYAPEGIDSALNSSYAIILAISAFGLIIMYRAVPDDPRTH
                     GLAGACSKEQGIVRRMEHLVVPITAAIALLLVLLGVNAGIIFLLAGLMLMPLGIIGYI
                     DDANIVNRDNDFATFIRSLGAIMGGKGITTGDALREVDRKSLIHLDPFIASVATKLNL
                     GLDEAGSWTKFIGETGSYLIYKYMHIFRDAVALGGSPDAIGKIVGSSMLEQVLLRRKR
                     DMMVKGFVVLLIPMHGAMIGIFAFLFEVLLSMSRAVTEVMEHFAETSAALSGGTSTIG
                     GAMSSSLNIFANFPEDTMRAYVVTILLMLTVANTFAGKIVMGGDRYMYYFFASLLSAV
                     TGVIYIVAPIIVGIFFNIPSFAGV"
     gene            6173..7198
                     /locus_tag="PLV90_04510"
     CDS             6173..7198
                     /locus_tag="PLV90_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96409.1"
                     /translation="MREGLRRALLFLITIGMGSLIILVDAPVEVILAGTVLAGFFALV
                     ITGALNLAELRPSRLRKALRERKKPPEAKKPDIPAEKPPRIRGSGMLGTLAASVRETI
                     AHARAPEVEKKDRMGKIDAMLDEAVDGTAPKKPAVPAKAGGGAVDPLASLADLDIDAL
                     AEIDQDGESPGPGTAIESDRISADAVSEILKAHQNDLDGINLTPGIDLAGNPDAALPA
                     IAGAPGGDGMPDLSALNEELSLLDDLDLDEIEIEGEEKEEIRAEEPEEAPEEAEEPEE
                     EFDMVSFASGGTVDDDLITSLKSDAKKKKFVEDVSLVRDLKGEKYDARSLALELEEIL
                     AAMRSQR"
     gene            7228..8034
                     /locus_tag="PLV90_04515"
     CDS             7228..8034
                     /locus_tag="PLV90_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheF family chemotaxis protein"
                     /protein_id="HOF96410.1"
                     /translation="MKSVPVKVEHEGRWVPTTMGIGEDRIRIAAPLDCEITYKSVVDL
                     EEKKNQVTITAGTDKDLVYRIASVEKVLSVLKKFIITQASAYRLNAFFMSPAIRGGVL
                     IQDARWEKGAIAVMKTGIWFVGQETQVCIPLADVAGIELTSREIQEKNLDVVKIDHLI
                     ENEVVTSFVLCPMTTLQVLYNFLKEATQDSDVSEELDPLTGQVAMLVYSGMDSSAIEN
                     MLKLSHKDLETVYDRLLGTGLAEVLYIRKEVQLTAKGVRYISNAMKSSSN"
     gene            8104..8466
                     /locus_tag="PLV90_04520"
     CDS             8104..8466
                     /locus_tag="PLV90_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOF96411.1"
                     /translation="MGRILIVDDTMFMRTLLKNILFSGGHDIVGEAADGAEAVARYQE
                     LRPDLVTMDIVMPKVNGIEALKAIKAADPAAKVIMCTAVGQEQMVKLAVKSGARGYIV
                     KPFQAPKVLEEVKNVLNA"
     gene            8470..9489
                     /gene="cheB"
                     /locus_tag="PLV90_04525"
     CDS             8470..9489
                     /gene="cheB"
                     /locus_tag="PLV90_04525"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HOF96412.1"
                     /translation="MIRVLIVDDSLFIRTILRDLLKDSPEIEIVGTAVNGIDALAKIA
                     DLKPDVVTLDIEMPRMNGLEVLEALRKERVRPKVIVLSTLTSRQADMTHRALRLGADD
                     FMLKPRDVPNVRGIERELIQKIKNLVSLPVIVRSSTAPKGKADAVVLIGSSAGGPPML
                     DAVLSALPADLPAAVVVTQHMPEGFTASLAERLNRVSPLPVRETANGDTLQTGTVLIA
                     KAGYHTVISGVYAGAQKTAGRVVHSTAPPLHAVRPAVDMTFTSAARVFGPRTVSVILS
                     GMGSDGGEGMLALKNAGGSTMVCAEEDCLVYGMARSALARNCVDRVVPLKNLAHEIAR
                     AVSRL"
     gene            9499..11406
                     /locus_tag="PLV90_04530"
     CDS             9499..11406
                     /locus_tag="PLV90_04530"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOF96413.1"
                     /translation="MFDEEIYRKLFVEESRENHENIVTNLLALESGEDQQTAIDEIFR
                     SAHTLKGMSASMGFDAMEHLCHSMEDVFSLIRSGRQEVTASLTDLLLTCTDTIEGMLD
                     AIEAGGTPSDERSGSLVQDLRAFAEVETPSGEQEASVEAVPAEEAGGRPLYTLSIGVD
                     PSCTMKDVRAMLLLQNLEGIGTVLSTTPPRSLLEDGKFENSFEVLIESDAGEDAIRTV
                     ASGTEIALLALSPLLPEAACPPEPEEEAERLQRPAKAEKSREIKNIRVDIERLDRMMN
                     LVEDLVINRGRLEQIAKKHGIKEFDEALNMVGRSVSDLQNLMMGIRMMPLERIFNRLP
                     RVVRDVANHDGKEVELVIEGGETELDRSMMDGLSDPLLHIIRNAVNHGIETPNIREAA
                     GKPRKGLLRLTARRDKDNVVIDIEDDGAGIDTERLRKKAVEKGLMAPETAAAATKDDL
                     IALLFEPGFSTAETITDISGRGVGLDVVKKTIESLKGTIKVESALGAGTKFELVLPPT
                     MAIIDVMMVHINGMRCAIPISNVVEVAQMRMDAVHRIGDSEAILLRDQILPVHRLEDM
                     FGRTRRSDTLVVLQNNSRRCCILVDTVDGQQEVVVKPLSRIAGNCRGISGITIPGDGD
                     VVPVLDVNTMV"
     gene            11418..12032
                     /locus_tag="PLV90_04535"
     CDS             11418..12032
                     /locus_tag="PLV90_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HOF96414.1"
                     /translation="MELDPIQMDALAELGNIGAAHAATTLSQMLMCPIDMTVPEVHAV
                     DIADMHSYIGNEISAVVVFQIQGEVADGGYIVVSMPRATVIQLTNQMLGMTDTDREID
                     DMDRSAAIEIGNIMISAFLDATAELLGIVMLPSPPALAIDMTHAAFESIVAQVAGDVN
                     DILIFNTELESEAPPVYGSIYMLPNTELMHQLFHMLERLMEPIS"
     gene            12050..12541
                     /locus_tag="PLV90_04540"
     CDS             12050..12541
                     /locus_tag="PLV90_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOF96415.1"
                     /translation="MENNFFSEVKAVILGLGELRVGTCPMGTIGLGSCVALILHDQRR
                     SLGGLAHIMLPESRGSVDRPGKFADTAASTLLAEMEMCGSSKFAIAATLVGGASMFEF
                     SANSLNIGERNIVAVRDVLRDQGIRIVHEETGGRIGRSVYYWPEGKGRIVVKRADGTC
                     ARL"
     gene            12526..13701
                     /locus_tag="PLV90_04545"
     CDS             12526..13701
                     /locus_tag="PLV90_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96416.1"
                     /translation="MRQTLIFFFILSSLLLSPSSAVSVDVPDFHAWIASAGVTDAING
                     RYGDVIFATDTGISFYGANGTWHSVNARFPGETAYGLLSPLDSMVTAIALDAGGHLWI
                     GYPNGLQIGDGKRYQSIQDQQFLKNLNIGCIVRWGDDMWVAAGRAGLHRYHDGAWTWY
                     KPLGPEGLGCYTVVSMAVDAASDALVVGSEQGGLRVLRNRTGEVRFEPVTCDDEPVRG
                     ISEVRVDPFGGVYLFNRTTVLHYDPGGKVTPILDTGDLNPFPVAINDLAATSGGMLLI
                     ATDDGIYGWNSSGVALHIASGDGIRSNVVKKLFIDADGRCWFVVPGNVGYIPPTAGHA
                     TLDLAAAPVLAPHEPETPAIETPTADIPSVEPAGVPDMLSGAWASFVEWVNARLAGA"
     gene            complement(13717..15489)
                     /locus_tag="PLV90_04550"
     CDS             complement(13717..15489)
                     /locus_tag="PLV90_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis/translation initiation
                     ATPase RLI"
                     /protein_id="HOF96417.1"
                     /translation="MRIAVVHRDRCHPVKCGTECILYCPRVRTGDETVVIGEDQKAVI
                     SEELCVGCGICVKKCPFEALAIVNLPEELDQPTHRYGKNGFVLYGLPIPIEGKVTGIL
                     GPNGIGKSTSVKILSGTLVPNLGRTDAPVSWKEVLDLYQGTELFDYLQVLSQKNVKVA
                     VKPQYIDQIPKVFTGSVRDLLATTDERGKLGYYIDRLSLKAVVDRPITNLSGGELQRV
                     AIAACLARDADFYFFDEITPYLDVYQRMAAADLIRELARERPVVIVEHDLAILDMLAD
                     TVHIAYGEPAVFGVITYPKGVRVGINQYLEGFLPEENVRFRPYAVTFDARAHAAGTDR
                     EVLLTFPAMTKQFTQFSLSVGGGEIRSGEVLGVLGANGIGKSTFAGLLAGVIEPDTGS
                     MDTRVRISYKPQYLKGDTDATVEEVLRQTTTKFDTSFYQHEILEPLSLSPLLQAPVNT
                     LSGGELQRVAIAVCLSRDADLYILDEPSAHLDVEQRVKISRMIRKHAEGRGASTLVID
                     HDIYVIDMISDRILVFEGEPGIRGKAVGPFDMAPGMNRFLSALGVTFRRDRSGRPRIN
                     KPGSFLDREQVAAGEYYYTEISKS"
     gene            complement(15496..15858)
                     /locus_tag="PLV90_04555"
     CDS             complement(15496..15858)
                     /locus_tag="PLV90_04555"
                     /inference="COORDINATES: protein motif:HMM:NF039857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96418.1"
                     /translation="MSDNVVAAEVDKTRPRTRAEKQVEHRNRIKRTITACFMGILAGG
                     LSFYLSGTPDPVSGLQANGIIGLFLLAAGIVFQKYIFVLLRIDYMELTGKDWFYQGFM
                     TLALWFMTWTILLTTTVL"
BASE COUNT         3349 a         5082 c         4807 g         2767 t
ORIGIN      
        1 ggacataccc cgcaccgctc agggttgctg ctgagagcac ccgtagaccc atctccacat
       61 atcacgcagt cgtcaccgcg actcgcacag cgaaccaccc ccccacaacc cccataaccc
      121 cccaaacaca caggaaaacc cctatatcca gggcaaaaat ttataaccca atacgattat
      181 ataatccact gttatggtgg gggtagacca ggctcagatc gacaaaatac tcgccggcgc
      241 atcttccgac gacccggaag cccgtctcga tacgctcgag aaggagctgg acttcgtcaa
      301 gacatcgatc aagcggctgc tcatcgacct ccgggagcgg atgaacgaac tggacaaccc
      361 gttcacctgc gcagcaccct tcggagggag ccggctcccg ccgccgacac tccccgccgg
      421 catcgaggac gacgacctgc ccgggctgga catgctgccc cccggcctcc cgacgaagaa
      481 ggacgacctc tcgtttcccg gcctcccgac gaagaaggac gacctcccgt ttcccggcct
      541 ccaaaagaag acagacgacc tctcgttcgc cgcgggcctc ggggatgccg gtctcttccc
      601 acccgatatc aaaccggcac ccccggatgc aaaatccccg ggaaaactca aactccagaa
      661 ggtccaccgg ctctttgcgt gggtgcacca gggctgttcg aaatacgggc acgaacacat
      721 gacgatcatg atcgacgcct accggtcgat gggctacatc accgatgagg tcgccgacca
      781 gatccgcgag atcatgcgga tggcccccga gaccaggaag gatatcgccg atatcggccc
      841 gaacgagttc gtctcggagc tctatgtctt gaaccggatc ctcgaccccg aagacgcgac
      901 gatggaccgc gacctcatcg aggtgctgat gctcgcccgc cgccgccagg ggaaaccgga
      961 cgcaccgtcg cgggatgccg acgacgcctg gatagaactc ctggacagga tatgatgaat
     1021 gtcaagcgag accttcacca ccgcgatgtt cctcatcgcc gccatcatat cggccggcgt
     1081 cctcattaac gcagtctttc ccgtggtcta caccctcgcg ggcacgatcg cctcgtcctc
     1141 ccacaaggtg gacgaacggc tgagcacgga cgccaggatc gtcacgacct acgcaagcga
     1201 agacaaccgc atcgcccgca tatggttaaa gaacgtcggc accgggagga tagccgacgc
     1261 cgacgtgcgg aaggccgacg tcttcctcgg gggccagggc gacttcatcc ggttgacgtg
     1321 gaagtcaggc gaaccgctcg cgtacggaac ctggacccac aggatactcg aagagaccca
     1381 gaacaactac tgggaccccg gcgagaccct ctacatcgag ggaatggccg cagaagtccc
     1441 ggcaaaggga caggtcgtct acttccagtt cgtcctcccc acggggctct cccggtcgac
     1501 caccttcacc gcgagtgatt agccatgagc gcaggacccc tcatcgcatc agggatcggc
     1561 gtcatcctcc tggtcgccac cgcctacgtc ctcgtcggcg gcaccctcgc caccacggag
     1621 gtgatggtcg aggcgcagaa cagcctcgtc gcccagcagg agatccgcat gcggacggcg
     1681 atcacgatcc aggatgcggc gatcgaaggc cagaccctgt acgtcgatct gaagaacacc
     1741 gggagcgaac cgatcgtcga cgtcgccgca atcgacgtct acatctaccg cgacggcacc
     1801 ccccactaca ccggagactg gacggtggtg gacatatccc ccgacgtcat ccaccccggc
     1861 atgctcgacc ccggcgagac actcttcctc gccgtcaggt tcgaagggaa cagcccggga
     1921 tacgtccagg tcgtcacggg aaacggcgtc tcgagttccg catacgtgag gtgaagaggc
     1981 atggccacag atagcagcat acccgggtta cccgaccgat cgatcctctc gaccgggaac
     2041 tccgaactcg acaagaagat cgccgacggc ctccccctcc aatccctcac cctcatcgag
     2101 ggggagaacg acaccggaaa gagcgtcctc acccagcaga tcatatgggg cgctctaaaa
     2161 caggggttca acgtcgacct cttctcgacc gagaacacga gcaagagctt cctctcccag
     2221 atggagtcga tgagcctcga catatccgac tacttcgcct ggggctacct ccgggtcttt
     2281 cccatgcacg tcgtcgggtt cgagtggacg aaagagaaga tgcaggggac gcttgaacgg
     2341 atgatcgcct acatggagca gagcaaagcg caggtgatcg tcatcgactc cctcaccctc
     2401 ttcaccgagt acgccaagca ggacaccgtc ctcacgttct tcacgaactg caaaaacctc
     2461 gtcgaccacg gcaagaccat cctgatcacc ctgcacacct acgccttcgt cgaggactcc
     2521 ctcgtccgta tccgctccat ctgcgacgcc cacctcttca tgaagaaggc gctcgtcggc
     2581 ggcaagtacg tcatgatgct cgaggtcgtc aaggtccgcg gcgcacgaaa gaccaccggg
     2641 aacatcatca gtttcgaggt ccaccccggg tacggcatca agatcatacc cgtctcggtc
     2701 gcgaaggtgt gagaagcatg agttcggcgc tggaagcaat gataaccctc cccttcgagc
     2761 ccgagttcat cgacgagggg aacgactgct acaacaacgt cgagtcctgc gtcctctacc
     2821 gcatgctccc ggcaaacgca aaagactacg tcagggcaag cccccacctc ctcgagtacc
     2881 tccacatcct gccggtcaac accgtcggca taccgctctt cctctcggag ctgaagcggg
     2941 acctcaaatc gatggagaac ccgaacatca tctaccccgt caacgagacg acgttcgtcc
     3001 acatcttctc ggaccctaac gacgtccgga actggtacat accgatcgaa ccctcgttcc
     3061 tccactccgt caaagagatc gtgccggtga tcgaggcaaa actgatcgac atgatcgacg
     3121 ccctcgacga ggagccggtg accgaagagg cgcgcatcga ggtgctcaag aacttcatcc
     3181 ggcagctcgt ctacgtccgg cagccgggcg aggaggttga cccgtccctg cttgccggcg
     3241 cagcggcaaa agacatcaag tcccggatca agaccttcct cgtctccgac atcggtgcgt
     3301 cggcaacccg gcaggctcac ggccacccca ccctcgccga cggccggctc atcctcaccg
     3361 cgcaggagta caaggcgctc gaatacctca tggtccgcga caagatcgag atggggacgc
     3421 tcaaaccctt cctctccgac aaatacatcg aggatatatc ctgcaacggc gtcgggccga
     3481 tattcatcga gcacaagata ttcaaaggct tgaaatcggt cgtcgggttc aaagactcag
     3541 gggagctcga caacttcgtc atcaaactcg ccgaacggat caaacggccg ctgacctacc
     3601 ggagtcccat cgtggacgcg accctccctg atggttcgcg tatcaacatc gtctacggga
     3661 ccgagatcag caaacacggc agcaacttca ccatccgtaa ggtgaacgag gtccccttaa
     3721 gcatcctcca ggtgatcgag agcggggcgt gcgactacat gatggccgcc tacctctgga
     3781 tctgccttga gtacggcatg tcgatgttcg tctccggcga gaccgcaagc ggcaagacga
     3841 cgaccttaaa cgccctgacg accttcctcc cgcccgagaa caagatcgtc accatcgagg
     3901 atacccccga gctgaccgtc ccccaccgga actggacccg cgaggtcgcg aaggccaagg
     3961 ggaagggcga aggcgagggc tcggagatca cgatgttcga cctcctcaaa gccgctctgc
     4021 gccagcgccc gaaccagatc ctggtcggcg agatccgtgg cgtcgaaggc tccgtcgcgt
     4081 tctccgccat gcagaccggc cacccggtga tgagcacctt ccacgccgcg tcggtcgaga
     4141 aactgatcca gcgtctctgc ggagacccga tcaacatacc aaagacctac gtcgacaact
     4201 taaacctcgt catcatccag agcgccgtca aactcccccg gggcgggacc gcccggcgga
     4261 tgctcagcat caacgagctc gtcggctacg accccgagac ccaggggttc tccttcatgg
     4321 cggcgttcat atgggatccg gcaaccgaca tgttcacctt caccggcagg gggagcagct
     4381 acctgctcga gaacaagatc gcgacgatgc tcggcatacc cgagaaccgg cgagccgaga
     4441 tctacgacga gatcgataaa cgggcaaaga tccttgagcg gctccacaag gccgggtaca
     4501 cccagttctg ggacctcttc cacatgacca cgaagatcaa gaagcagggc ctgctcacca
     4561 tcgaggtgtg atgtgttcga ggacgtcgcc gagcgtctgc gggcggcaaa ccaggggaag
     4621 ataccgtttg aagaggaggt cgggcgtctc accgacctcc ggtcctccat cctcgagaac
     4681 aagaagatgg agcaggacct gctcctcatg tacacctaca tggccgcgat caccacgtcg
     4741 gccgtgaccc ggcccgagat ctttcagtac acgtccgagc ggatagagta catcccgtcg
     4801 cgctacatcg cccaggtgca gcgcctcgtc gccgggtggg gccacagtta cgccggagcg
     4861 ctccgggcgg tcgctgaacg gtgccgtaac gacaccctcc agagcatgct caaccgctac
     4921 gcgaactcga tcgactccgg cgtccccgac gacgccttca tcgagacaga gctctcgagc
     4981 atccggagca tctatcgaaa ctcgttcgag caggggctcg aactcctgaa gaagtggggc
     5041 gacgcctaca tcgccatgct cctctcggga tcgctcgttg cgatcatcat catgatatcg
     5101 gtcgctatct acgctccgga agggatagac tcggcgctga actcctctta cgcgatcata
     5161 ctcgccatat cggccttcgg cctcatcatc atgtaccggg ccgtccccga cgacccccgg
     5221 acgcacgggc ttgccggggc atgctcaaaa gaacagggca tcgtcaggag gatggagcac
     5281 ctcgtcgtgc cgatcacggc ggcgatcgct ctcctgctcg tcctcctcgg cgtcaacgcc
     5341 ggcatcatat tcctgcttgc cggcctcatg ctgatgccgc tcggcatcat cggctatatc
     5401 gacgatgcga acatcgtcaa ccgggacaac gactttgcca cgttcatccg gagcctcggg
     5461 gcgatcatgg gcggcaaagg catcaccacc ggcgacgcgc tcagggaggt ggaccgaaag
     5521 tcgctcatcc acctcgatcc gttcatcgcc tccgtcgcga cgaagctgaa cctcggcctc
     5581 gacgaggccg ggagctggac gaagttcatc ggcgagaccg gcagttacct gatctacaag
     5641 tacatgcaca tcttccgcga cgccgtggcc ctcggcgggt ctcccgacgc gataggcaag
     5701 atcgtcggtt catcgatgct cgaacaggtg ctgctccgga ggaagcgcga catgatggtg
     5761 aaggggttcg tcgtcctgct cataccgatg cacggggcga tgatcggcat cttcgccttc
     5821 ctctttgagg tcctcctctc catgtcccgt gcggtgacgg aggtgatgga gcattttgca
     5881 gagacatcgg ccgcgctctc gggcgggaca tccacgatcg gcggcgccat gagttcgtcc
     5941 ctcaacatat tcgccaactt cccggaagat accatgcggg cctacgtcgt gacgatcctc
     6001 ttaatgctga ccgtcgcaaa cacgtttgca ggaaagatcg tcatgggcgg cgaccggtat
     6061 atgtactact tcttcgcaag cctgctctcc gcagtcaccg gcgtcatcta catcgtcgca
     6121 ccgataatcg tgggcatatt cttcaacatc ccatcgtttg cgggggtgtg atatgagaga
     6181 gggccttcga cgcgcgctcc tctttttgat caccatcggc atgggctcgc tgatcatact
     6241 cgtcgatgcc ccggtcgagg tcatactcgc aggaaccgtg cttgcagggt tctttgcgct
     6301 cgtcatcacc ggtgcgctca accttgcgga gctcaggccc tcgcggctca gaaaagccct
     6361 cagagagcgc aaaaagcccc cggaggcaaa gaaacccgac ataccggccg aaaaaccgcc
     6421 ccgcatccgg ggatccggca tgctcggaac gctcgcggca tcggtccggg agacgatcgc
     6481 gcacgcccgc gcccccgagg tcgagaagaa ggatcggatg ggaaagatcg acgccatgct
     6541 cgatgaggcg gtcgacggta cggccccaaa aaagcccgcg gttccggcaa aggccggcgg
     6601 gggcgcagtc gaccctcttg cctcgcttgc cgacctcgat atcgacgctc tcgcggagat
     6661 cgaccaggac ggcgaatcgc ccggtcccgg gacggcgatc gagtccgacc ggatatcggc
     6721 ggatgccgta tcggagatac tcaaggcgca ccagaacgat ctcgacggca tcaacctcac
     6781 gcccgggatc gatctggccg gcaaccccga tgcagccctc ccggcgatag ccggagcccc
     6841 gggcggcgac ggcatgccgg acctgagcgc cctgaacgag gagctctctc tccttgacga
     6901 tctcgacctc gacgagatcg agatcgaggg cgaggagaag gaggagatcc gcgcagaaga
     6961 acccgaagag gccccggagg aggcagaaga gccggaagaa gagttcgaca tggtctcctt
     7021 cgcatccggc ggcacggtcg acgacgacct gatcacatcg ctcaaatccg atgcgaagaa
     7081 gaagaagttt gtggaggacg tcagcctggt ccgcgacctg aagggagaaa aatacgatgc
     7141 gaggagcctt gctctggaac tcgaggagat cctcgcggcg atgcgatcac aacgatgaag
     7201 ttctcgcggg agatatcata gagagcgatg aagtctgttc cggtaaaggt cgagcacgaa
     7261 ggcaggtggg ttccgacgac gatgggtatc ggcgaggacc gtatccggat cgccgctccc
     7321 ctcgactgcg agatcaccta caaatccgtg gtcgatcttg aggagaagaa gaaccaggtg
     7381 acgatcaccg ccggcacgga caaagacctg gtctatcgca tcgcgtcggt ggaaaaagtc
     7441 ctctcggtct tgaagaagtt catcataacc caggcgagcg cgtaccgcct gaacgccttc
     7501 ttcatgtcgc ccgcaatccg cgggggggtg ctcatccagg atgcccggtg ggagaagggg
     7561 gcgatcgccg tcatgaagac cggcatctgg ttcgtcggcc aggagaccca ggtctgcatc
     7621 cccctcgcgg atgtcgccgg catcgagctc acgtcgcggg agatccagga gaagaacctc
     7681 gacgtcgtga agatcgacca cctcatcgag aacgaggtcg tgacaagttt cgtcctctgc
     7741 ccgatgacga cgctccaggt cctctacaac ttcctcaaag aggcgacgca ggactccgat
     7801 gtcagcgagg agctcgaccc cctgaccgga caggtggcca tgctggtcta cagcggcatg
     7861 gactcaagcg ccatcgagaa catgctcaaa ctctcgcata aagacctcga gaccgtctac
     7921 gaccggctcc tcggcacggg gctcgccgag gtgctctaca tcagaaaaga ggtgcagttg
     7981 accgcgaaag gcgtccggta catttctaat gcgatgaaat cctcatcgaa ttaataatac
     8041 cttttctcaa gaacaacaag gcctatatag cgcgttctcc aaataatcat caagtggttt
     8101 ttgatgggga gaatcctgat cgtcgatgat acaatgttta tgagaacgct gctgaagaat
     8161 attctcttct ccggcgggca cgacatcgtc ggtgaggcgg ccgacggggc ggaggccgtc
     8221 gccaggtacc aggaactcag gcccgacctc gtcacgatgg atatcgtcat gcccaaggtg
     8281 aacggtatcg aggcgctcaa ggccatcaag gccgccgacc cggcggcaaa ggtcatcatg
     8341 tgcaccgcgg tcggccagga gcagatggtc aaactcgccg tcaagagcgg ggcgagaggc
     8401 tacatcgtca aaccgttcca ggctccaaaa gtcctcgaag aagtcaaaaa cgtcctcaat
     8461 gcgtgagtta tgatacgggt tctgatcgtc gacgattcgc tcttcatccg gacgatactc
     8521 cgggatctcc tcaaagatag cccggaaatc gagattgtcg gaacggcggt caacggcatc
     8581 gacgctctcg caaaaattgc agacttaaag cccgacgtcg tcaccctcga tatcgagatg
     8641 ccccggatga acggcctcga ggtgcttgag gcgctccgga aggagagggt caggccgaag
     8701 gtgatcgtcc tgagcacgct gacatcaagg caggccgata tgacccaccg ggcgctccgg
     8761 ctcggggcgg acgacttcat gctcaaacca cgggacgtcc cgaacgtcag gggaatcgag
     8821 cgggaactca tccagaagat caaaaacctc gtctcgctcc cggtgatcgt gcggtcgtcc
     8881 acggccccga agggcaaggc cgatgcggtg gtcctgatag gctcgtcggc cggaggcccg
     8941 ccgatgcttg atgcggtcct ctccgcgctc cccgcggacc tgcctgcggc ggtcgtcgta
     9001 acccagcaca tgcccgaggg gttcaccgca tcgctcgccg aacgcctgaa ccgggtctcg
     9061 cccctcccgg tgagagagac cgcaaatggg gataccctgc agaccggaac cgtcctcata
     9121 gcgaaggccg gctaccacac cgtcatatcg ggggtctacg ccggcgccca aaagaccgcc
     9181 gggagggtcg tccactcgac cgctcctccc ctccatgcgg tccggcccgc cgtcgatatg
     9241 accttcacat ccgctgcgcg cgtctttggc ccgcggaccg tctccgtgat cctctcgggt
     9301 atggggagcg acggcggcga gggtatgctc gccttgaaga acgcgggcgg cagcacgatg
     9361 gtctgcgccg aagaagactg cctggtctac gggatggcac gctccgccct tgcgcggaac
     9421 tgcgtcgaca gggtcgtgcc gctcaaaaac ctcgcacacg agatcgcacg agccgtcagc
     9481 cggctttagg tgagatccat gtttgatgaa gagatatacc ggaagctgtt tgtcgaggag
     9541 tcgagggaga accacgagaa catcgtgacc aacctccttg cccttgagag cggggaagat
     9601 cagcagaccg cgatcgacga gatcttccgg tccgcgcata cgctcaaggg gatgtccgca
     9661 tcgatgggtt ttgacgcgat ggagcacctc tgccactcga tggaggacgt cttctcgctc
     9721 atccgcagcg gccggcagga ggtgacggca tctctcaccg atctgctgct cacctgcacc
     9781 gatacgatcg aggggatgct ggacgcgatc gaggccggcg ggaccccgtc ggacgagcgg
     9841 tccgggagcc tggtgcagga tctccgggcg ttcgcagagg tcgagacccc ctccggtgag
     9901 caggaagcat ccgtggaagc ggtgcccgcc gaagaggccg gcggccgccc gctctacacg
     9961 ctctcgatcg gcgtcgaccc gtcctgcacc atgaaggatg tgcgggccat gctgctcctg
    10021 cagaaccttg agggtatcgg gacggtcctc tcgacaaccc ccccgcggag cctcctcgag
    10081 gacgggaagt tcgagaactc gtttgaagtc ctcatcgaga gcgatgcggg cgaggatgcg
    10141 atcaggaccg tcgcctccgg taccgagatc gcccttctcg ccctctcccc gctcttgccg
    10201 gaggcggcct gcccgcccga accggaagag gaggcagagc gactgcagcg cccggcaaag
    10261 gccgagaaga gccgggagat caagaacatc cgtgtcgata tagagcgcct cgaccggatg
    10321 atgaatctcg tcgaagacct ggtgatcaac cggggaaggc ttgagcagat cgcaaagaag
    10381 cacggcatca aggagttcga cgaggcgctg aacatggtcg gccgctcggt ctccgacctc
    10441 cagaacctca tgatggggat ccggatgatg ccgctcgagc gcatattcaa ccgcctcccg
    10501 cgggtcgtgc gggacgtggc aaaccacgac ggcaaagagg tcgagctcgt catcgagggc
    10561 ggcgagaccg agcttgaccg gagcatgatg gacggcctct ccgatcccct cctccatatc
    10621 atccgaaacg ccgtgaacca cgggatcgag accccgaaca tccgcgaagc cgccggaaaa
    10681 ccccggaaag ggctgctcag gctgacggca cggagggata aggacaacgt cgtcatcgat
    10741 atcgaggacg acggcgccgg catcgatacc gagcggctcc ggaagaaggc ggtcgagaag
    10801 ggtctcatgg cgcccgagac ggcggcggcc gcgacgaaag acgacctcat cgccctgctc
    10861 tttgaaccgg ggttcagcac ggccgagacg atcacggaca tcagcggcag gggcgtcggg
    10921 ctcgacgtgg tcaagaagac gatcgagtcg ctcaagggga cgatcaaggt cgagtccgcg
    10981 ttaggggcgg ggacgaagtt cgaactggtg ctgcccccga cgatggcgat catcgacgtc
    11041 atgatggtcc acatcaacgg tatgcggtgc gccatcccga tcagcaacgt cgtcgaggtg
    11101 gcgcagatga ggatggacgc ggttcaccgg atcggcgata gcgaagcgat cctgctccgc
    11161 gaccagatcc tgcccgtgca tcgcctcgag gatatgtttg gccggacgcg gaggagcgac
    11221 acgctggtcg tgctgcagaa caacagcagg cgatgctgca ttcttgtcga tacggtggac
    11281 ggccagcagg aggtggtggt gaaaccgctc tcccggattg ccggcaactg ccgcgggatc
    11341 agcgggatca ccatccccgg ggacggcgat gttgtgccag tgctcgatgt aaacacaatg
    11401 gtttaggaga gattttcatg gaacttgatc cgattcagat ggacgcgctg gcggagttag
    11461 gcaatatcgg tgcagcgcat gcagcgacaa ccctatcgca gatgttgatg tgcccgatcg
    11521 atatgacggt gccggaggtg catgccgtcg atatagccga tatgcacagt tacataggca
    11581 acgagatatc ggccgtggtc gtcttccaga tccagggcga ggtcgcggac ggagggtaca
    11641 tcgtcgtcag catgccgcgg gcgaccgtca tccagctgac gaaccagatg ctcggtatga
    11701 ccgataccga tcgtgagatc gacgatatgg accggagcgc cgcgatcgag atcgggaaca
    11761 tcatgatatc ggcgttcctc gatgcgaccg ccgagctcct gggcatcgtc atgctgccgt
    11821 ccccgccggc gctggccatc gatatgacgc atgcggcgtt tgagtccatc gtcgcgcagg
    11881 tagccggcga cgtgaacgac atcctgatct tcaatacgga actcgagagc gaggcgccgc
    11941 ccgtctacgg gagcatctac atgctcccga acaccgagct catgcaccag ctcttccaca
    12001 tgctggaacg cctgatggaa ccgatctcat aacgggcctc cgggatacca tggagaacaa
    12061 cttttttagc gaggtaaagg ccgtaatcct gggtctcggc gaactccggg tcgggacatg
    12121 cccgatgggg acgatcgggc ttggctcctg cgttgcgctc atcctgcacg accagaggcg
    12181 atccctcggg gggcttgccc acatcatgct cccggagagc cgcgggagcg tcgatcgccc
    12241 cggcaagttt gcggataccg cggcaagcac cctccttgcg gagatggaga tgtgcgggag
    12301 ctcgaagttc gcgatcgccg caaccctcgt cggcggtgcg agcatgtttg agttctcggc
    12361 gaactcctta aacataggcg agagaaacat cgtcgccgtg cgggatgtgc tccgcgacca
    12421 ggggatacgg atcgtgcacg aggagacggg cggcaggata gggcggtccg tctactactg
    12481 gccggagggg aagggccgca tcgttgtcaa gagagcggac ggaacatgcg ccagactctg
    12541 atcttcttct tcatcctgtc atcgcttctc ctatccccct cgtcggccgt ctccgtcgac
    12601 gtccccgact ttcacgcttg gatcgcatcc gccggcgtga cggatgcgat caacggccgt
    12661 tacggggacg tcatatttgc gaccgacacc ggcatatcgt tctacggagc gaacggcacc
    12721 tggcactcgg tcaatgcacg gtttccgggg gagaccgcct acggcctgct ttcgcccctc
    12781 gactccatgg tgaccgcaat cgccctcgac gcgggcggcc acctctggat aggatacccg
    12841 aacgggctgc agatcgggga tgggaaaagg taccagagca tccaggacca gcagttcctc
    12901 aagaacctca acatcgggtg catcgtgcgg tggggagacg atatgtgggt tgccgccggc
    12961 cgggcgggcc tccaccgcta ccacgacggt gcctggacgt ggtataagcc gctcggcccc
    13021 gaagggctcg ggtgctacac cgtcgtgagc atggcagtcg acgccgcaag cgacgcgctc
    13081 gtcgtcgggt cggagcaggg cggtctccgg gttctccgga acagaaccgg ggaggtccgg
    13141 tttgagccgg tgacctgcga cgacgaaccc gtccggggga tatccgaagt ccgggtcgac
    13201 ccgttcgggg gcgtctacct cttcaaccgc acgacggtcc ttcactacga cccgggcgga
    13261 aaagtcacgc caatcctcga taccggcgac ctgaacccgt tcccggtcgc catcaacgat
    13321 ctcgcggcaa cctccggggg gatgcttctt atcgctaccg atgacgggat atacggctgg
    13381 aactcatcgg gcgtcgccct ccacatcgcc tccggcgacg gcatccgctc aaacgtcgtc
    13441 aaaaaactct tcatcgatgc cgacggccgg tgctggttcg tcgttcccgg aaacgtgggc
    13501 tacataccgc cgacggcggg gcacgccacc ctcgatcttg cggccgcccc cgttctcgcg
    13561 ccgcacgaac cggagacccc tgcgatcgag actccgacag cggatatccc ctccgtcgag
    13621 cccgccgggg tgccggatat gctctcgggc gcctgggcat ccttcgtaga atgggtcaac
    13681 gcccggctgg ccggggcctg agggcggcaa accgcctcag gacttcgata tctccgtgta
    13741 gtaatactcc ccggccgcga cctgctcgcg gtcgaggaac gaccccggct tgttgatacg
    13801 cggccttccg ctcctgtccc gccggaaagt gacgccgagg gccgagagga atcggttcat
    13861 gcccggcgcc atatcgaacg gccccacggc cttgccgcgg atacccggct cgccctcgaa
    13921 gacgaggatc cggtcgctga tcatatcgat gacgtagatg tcgtgatcga tgacgagcgt
    13981 gctcgcgccc cggccttcgg cgtgtttgcg gatcatccgc gatatcttca cccgctgctc
    14041 cacgtcgagg tgggcgctcg gctcgtcgag gatgtagagg tcggcgtcgc gcgagaggca
    14101 gacggcgatg gccacccgct gcagttcgcc gccggagagc gtgttcaccg gcgcctggag
    14161 gagcggcgag agggagaggg gttctaagat ctcgtgctgg taaaacgacg tatcgaactt
    14221 cgtcgtcgtc tgccggagaa cctcctcgac ggtcgcatcc gtatcgcctt tgaggtactg
    14281 gggtttgtag gagatccgga cacgggtgtc catcgacccg gtatcgggct cgatcacccc
    14341 ggcaaggagc ccggcgaacg tgctcttccc gatgccgttc gccccgagga caccgaggac
    14401 ctcgccgctc cggatctctc cgccgccgac ggagagagag aattgagtaa actgcttcgt
    14461 catcgccggg aacgtgagca ggacctccct atccgtcccg gcggcgtgcg cccgggcatc
    14521 aaacgtcacc gcatacggcc tgaacctgac gttctcttcc gggaggaacc cctcgaggta
    14581 ctggttgata ccgacccgga cgcccttcgg gtaggtgatg acgccaaaga ccgccggctc
    14641 gccgtaggca atatgcaccg tatcggcaag catatcgagt atcgcgagat cgtgctccac
    14701 gataacgacc ggccgctccc gggcgagctc ccggatcagg tctgccgcgg ccatacgctg
    14761 gtagacgtcg aggtagggcg tgatctcatc gaagaagtag aagtcggcat ctctggcaag
    14821 gcatgctgca atcgccaccc gctgcagttc gccgccggag aggttcgtta tcggccggtc
    14881 gacgaccgcc ttgagagaga gccggtcgat gtagtagccg agtttgccgc gctcgtcggt
    14941 ggtcgcgagg aggtcgcgca ccgacccggt gaagaccttc ggtatctggt cgatatactg
    15001 cggttttacg gcgaccttca cgttcttctg cgagagcacc tggaggtagt cgaagagctc
    15061 ggtcccctgg tagaggtcga ggacctcttt ccaggagacc ggggcgtcgg tcctccccag
    15121 gttcgggacg agcgtgcccg agaggatctt caccgacgtg ctcttcccga taccgttcgg
    15181 tccgagtatg ccggtgacct tcccctcgat gggaatggga aggccgtaga ggacgaaccc
    15241 gtttttgccg tagcggtggg tcggctggtc gagctcttcg gggaggttga cgatggcgag
    15301 ggcctcgaac gggcacttct tcacgcagat gccgcacccg acgcagagtt cctcggatat
    15361 gacggctttc tggtcttcgc cgatgacgac ggtctcgtcg ccggtccgga cacgcgggca
    15421 gtagaggatg cattcggtgc cgcacttgac ggggtggcac cgatcgcggt gtacaacagc
    15481 gatacgcatg agggatcaca gaacggtggt ggtcaggagg atcgtccagg tcatgaacca
    15541 gagcgcgagc gtcatgaatc cctggtagaa ccaatctttg ccggtcaact ccatgtagtc
    15601 gatccggagg agcacaaata tgtacttctg aaatacgatg cccgccgcga ggaggaacag
    15661 gccgatgatg ccgtttgcct ggagcccgct caccgggtcc ggggtgccgg agaggtagaa
    15721 cgagagtcct ccggccagga tgcccatgaa acaggcggta atcgtccgtt ttatccgatt
    15781 tctatgctcg acctgtttct cggcacgtgt ccgcggccgt gtcttatcaa cctctgctgc
    15841 taccacatta tcgctcatcg tgacaccagt ctgaaccatt ccgggaaagg atgtctttcc
    15901 ggggcggtga accacgaagg gcaggctccc ggatcgacag aactactaca ttatagggtc
    15961 ttctccggtt ataaaggtaa accaccccac accccgggga gggat
//
LOCUS       DAOSII010000039        15812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_3883_length_15812_cov_46.424383, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000039 DAOSII010000000
VERSION     DAOSII010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 15812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15812
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3883_length_15812_cov_46.424383"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /locus_tag="PLV90_04560"
     CDS             <1..1277
                     /locus_tag="PLV90_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOF96419.1"
                     /translation="TNSTLRNPTHVFDHDGEGARTYTVTLTVTNSGGSDVKTTAITVV
                     APPQKEDVTDRVDQTNATATEDISVTYQGLTLEIPKGHDAKQADGSPITEFSVGVAPD
                     LEEPPAGTIEIGGKAFKLGPEGAKFNPKIPITITFTQDEWEELFGDGRTTKLQRYDGQ
                     NWIELENQTRDDTTFTITGYTDSFSVFAPVTTTTTPEPTSTPTQPRTSGSGSSTATVT
                     STGTAKLLTASWGGVLRPYLVYVDGKFAHLSLETGVTALDDAGSPLSEIGIAPVSTLP
                     GASSLAFSGYAVDCSPAGATFSPAIDLVFTFTGDEWAALLDRADGDSARLAVQGYDDA
                     TGTWTACPTNVDADARTVTASVSHFSTYVLFIGTATKVPVTTGPTPTVPAAAATTAPV
                     PPAETGESPLVWIALAVVIVVALAGYLLWKRR"
     gene            1426..4299
                     /locus_tag="PLV90_04565"
     CDS             1426..4299
                     /locus_tag="PLV90_04565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOF96420.1"
                     /translation="MPVFILVVALLVAPALAAGTTDVRIAKYAVDGTTVLNETTVDYH
                     WLKANLPVQGDGVTRYYHQGPVFEGHWEDAHPDEEYDGWNPAEDVLMSMLVKADLGAV
                     MGTDIADICELVGGAAEGDEIAVRSRDGWQSKRYPYEYIYDPDPRQGPAVLCWFSGNS
                     SGPDAQEAQGQGYPDTGYAVGMRMIFFADDTTNPWGWHIFGNNDMKECWDEEYWNKGG
                     DYWSAAGTSGKWVNEVRVYSQEEPEPPTPDFGANVTSGTVPLAVAFTDRSTGVPTPTS
                     WFWDFGDDTTSDEQNPTHTYTNPGTYTVTLEVTNVAGSDTAELSITATSGSDGGDTAD
                     TESPGSDNRTFLFEVPGIDEIVVFAAEIPQNLNLSCEEILLPDGIPEPPGAVYRYYNI
                     TSRNGTVAVDRAVIRFNVPDAWLQENWIVGDDLTLYRYNETWAPLETQWIGTENETNT
                     YEAVSPGLSLFAVSGIQIKPATAATTAPAPTPTPLATTPTGTATPAVSGTPAPAGKTP
                     ENPLVSIFGYTGVSTVGGLPGRLLGAVQAFLGLFGLLPAAADPVTEPEPVPTAPTPAP
                     VQAAPEPTPTPVDLTAMHFGLSVLSDPPGALIDLDGEYTGRTTPAAFASLPGGTHTVR
                     VRLDGAEPVEREIRLAHDDEVVIEIPVSASVRSASPGLLRGREQNHYGGVYVDSFPAG
                     AEIFVDGRKVNRKTPAVIYGLREGLHTIRVQEEAVAFSSGGEQVWIERNTVARMMFTG
                     GNEELKRSIAFESEEYAKQPFSVNGRYLGDRFPKTVEVSGIGEAYLAVRDGKSYRTYR
                     IPTAAGSGDTVNAIFSAAPCSILVTSTPAGAAIAIDGFQTGFATPYLVDNVSGGKHLV
                     SVSKPGYIPEEREILLTVGSGDATAKFILEPYTYGSLSVSSTPADARIYLFGRDTGEK
                     TPHTFHYLPIGSYPVKVTEVNRTVTEDVLIAPYQIARMEVPEV"
     gene            4481..5392
                     /locus_tag="PLV90_04570"
     CDS             4481..5392
                     /locus_tag="PLV90_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOF96421.1"
                     /translation="MIRPDQIIIVFLVLMLALLPAPALAAGAGTTELRIVKYANDRTT
                     VLNETTVDYRWLEANLPVQGDGVTRYYHQGPVFEGIWDEVHPDEPYDGWNPGEDVLMS
                     MLVKADFGAVKGTDLADICDSVGGASEGDEINLHSRDGFSKTFPYSIVYEPDSRQGPA
                     VLCWYSGNGSGSDAQEAQGQGYPDTGYTVGMRLIFFADDSTNPWGWHIFGNNDMRECW
                     GKAYWNYGGSYPSAAGASPKWVDEVRIYSKEDPAANHSAATRGPVEGGETPASLSPIL
                     GSVAALLAVLGVSFRYLRHEPDDDQSR"
     gene            5398..7209
                     /locus_tag="PLV90_04575"
     CDS             5398..7209
                     /locus_tag="PLV90_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOF96422.1"
                     /translation="MTTRCLSKILACCVLLLVCCMPATAGIPGDTDGDTNISADELAT
                     AILGYLGGTGALTAEELSLAAHNHLHQPYGQLVAAVLGETYLIPSTSYIDQKGVPADS
                     LIHEGLVTKLRPQDDGSGGYLGWLAERWEMSDDAMSWTFYLVKNATWHDGTPFTSADV
                     QFTYEYFRDHGDADAPAGSFLVNSAILSNVASVECPDPYTVVFRMKSCAPLFPETLAT
                     GPGIGVFPAHIWKDIDLPSTYVDSKQIGIGPFAYTSTIPGTLSKVTAYEGYHGEQPYV
                     REIVIRNYEDEDAAILALRRGDIDFISGEFGLSPRKAEALKGAPGIGITQIPSGGQAF
                     EVAFTSDFYPANITAFRRALSHAVNRERVCWLVGDGARPTETAFLLPALAGDQINPAT
                     NGRFSYDIEEAKRQLVAAGFNLTEANGRPVLLDPDGNRVEISIPLAGKASTNAVDQKI
                     VAVLKEDWEGKLGIRIVETPQVDDSIYLDWIARTAVHFDGMPGRWHEDIDRLDNFQRS
                     PLGENYYHFDNETFNRLITELKSTADPAERKEIGFRLQEILVEEVPCIPVFSQDAFMA
                     YRSDRFVGWDAPLLGGGGNIRLFAEIRPANAEVGLRA"
     gene            7259..8239
                     /locus_tag="PLV90_04580"
     CDS             7259..8239
                     /locus_tag="PLV90_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF96423.1"
                     /translation="MNGIYLLKRLATSFAAFCIAVTIMFVLLRLMPGDYITMYLESIY
                     RYAPPDVVETFNRVYGLDRPILEQYFTYLLDAFRGNWGYSFIYKVPVLTVIGEKLAWT
                     LIILLPATILATLAGIGIGAYSGWRRGSKADVGLLNGMIFIRAIPSYWLALMILIIFA
                     YYLNLFPLGGYVDTSALATGIDPFDVANHAVLPILTLFLFSVTGTYYLMRNSMLMTAG
                     EDYITTARAKGLDELTILRKHALKNAMLPMVTLITLECAGMITGSIFVETIFSWPGLG
                     MLTYKALAARDLPLLQGILLIDTLFVIAANIAADLIYPLVDPRVQVGDDA"
     gene            8232..9056
                     /locus_tag="PLV90_04585"
     CDS             8232..9056
                     /locus_tag="PLV90_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF96424.1"
                     /translation="MPRKKVILAASFGIVFAYCAVALLAPVIAPYDPHAITGAPLSPP
                     DKDHLLGTNDLAQDVFSGLIWGTRATLLLGFIGAAIAVAIGTAAGIVSGYYGGLIDEA
                     VMRLLDVAMTIPIFPLLLILTTFFRPGIYLIAVLMGLLGSFQGVRVIRSQVLSLAVAD
                     FVVGTRALGAGDGYIMARHILPGVLPLAGVKFVTSAQHFMLIGIGLGFLGITDPSVVD
                     WGQMINRASQNGAMVLGLWWWLIPPGLAVTLISLALAWLGYACEDEINPRLRVMRI"
     gene            9053..9997
                     /locus_tag="PLV90_04590"
     CDS             9053..9997
                     /locus_tag="PLV90_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF96425.1"
                     /translation="MTNEALLEVRGLKVGFPTPDGLVRASDTVDLEIYPRETLGLIGE
                     TGSGKTVLGMAILRLLQPNAVIEGEIRYRDHDLLTLPEDEMRKVRGREIGIVLQNSGT
                     SLNPVYTVGTQIAEAVTLHQGLSGHDARQRAVELLDAVGIPDPVRMAGEYPHRFSGGM
                     RERALIATALACRPTLLIADEPTKGLDAVTKRGIVDLFSEIDRDRSVLFITHDIETVE
                     DLAARVAVMYAGEIVEVGGVREVFGDPAHPYTRGLLASLPERGMRPIPGSAPSLITLP
                     PGCRFSPRCGCAIGGCNREHPGLVEVGPGHYARCMLYD"
     gene            9990..10844
                     /locus_tag="PLV90_04595"
     CDS             9990..10844
                     /locus_tag="PLV90_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF96426.1"
                     /translation="MIEVTDLETNFSTGFIHRRVVRAVDGVSFWIEEGETLGLVGESG
                     CGKTTLGRSILRLIEPDRGRIVVDGTDVTALSRSELRRFRPRMQMIFQDAYASLNPRM
                     RVSESVAEPLKIHRMGSRADIDRRVRELIDEVGLNEEHLNRRPYELSGGQNQRVALAR
                     VLALKPKFLVADEPTSALDVSVQAQILNLIRDLGRDYRMTCLYISHDLGVTRAMSSRV
                     AVMLEGRIVEIGRTADVFDRPCHPYTQRLVDAALAGRARAPPERPPLPAAPTMVEVRS
                     GHWVEETV"
     gene            complement(10860..11426)
                     /locus_tag="PLV90_04600"
     CDS             complement(10860..11426)
                     /locus_tag="PLV90_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96427.1"
                     /translation="MVNNPVRVSRGLVPICCILILASFCVVPAWASDAAVSREIDRSS
                     VPVGGEVLVTLAVDDLGVGGVVETIPEGFVFLETSFPEDRYSVSGQRLTFAVIDERKI
                     TYRVQAKAAGSWTFSGTWVDAVDKTEGAISPTAVTVGSGAAAAGAAPAGTGQAPGPTS
                     AGGAPWAVVPALAAAAVAAGACAARKER"
     gene            11655..12200
                     /locus_tag="PLV90_04605"
     CDS             11655..12200
                     /locus_tag="PLV90_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase subunit
                     gamma"
                     /protein_id="HOF96428.1"
                     /translation="MKELRIHGRGGQGSVTAAELIAFAAFEGGAYAQAFPAFGVERRG
                     APVQAFVRFSDAKIRLRSQIYEPDYIIVQDSTLIGDVNVFMGMRAGGIAIVNTEKPDF
                     DSRVPEGVKVYTVDATTIALEELGVPITNTTLMGAFAAATGEIGLEPLKHALQRRFSG
                     SMAEKNIRAAERAYNLIGGAA"
     gene            12200..12460
                     /locus_tag="PLV90_04610"
     CDS             12200..12460
                     /locus_tag="PLV90_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOF96429.1"
                     /translation="MALRVGCAAPPGRARDNKTGTWRVFKPVFDPETCNRCGMCAMVC
                     PEGCVRETEEGTFEPDLDYCKGCGICEEICPKKSIRMEKEEK"
     gene            12460..13614
                     /gene="porA"
                     /locus_tag="PLV90_04615"
     CDS             12460..13614
                     /gene="porA"
                     /locus_tag="PLV90_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HOF96430.1"
                     /translation="MLEFMEGSHAVAEIVKRCRPQVISAYPITPQTHIVEDLAQMVAN
                     CELDAEYITVESEFSALSACLGASAAGSRVYSATTSQGLALMFEVCFNVAGMRQPIVM
                     TIANRSLGAPLSIWNDQQDSISLRDSGWLQFYAEDNQETADLHMIAYKVCEDHDILLP
                     AFVCFDGFILTHTYEPVSLPTQEEVDAYLPAFTPYQRLDAANPLSMGMYATPEYYMEF
                     RYEIDRAMQRARQSFIKAGREFGEQFGRDYSGLVEGYRLEDADTALIAMGSICGTVKD
                     AIDEMRDAGKKVGLLKIRTYRPFPAPEIVDALKGVSTVAVLDKNISLGNGGAVGTEVK
                     SALYGSGVTVYDYIIALGGRDVRKRDIAAVVDLAEKGKGDMFYGLRTEVL"
     gene            13615..14523
                     /locus_tag="PLV90_04620"
     CDS             13615..14523
                     /locus_tag="PLV90_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOF96431.1"
                     /translation="MAEKEQGCELFSAGHRACGGCGPALAARLLLKATGKNVIIAAST
                     GCMEVFSTPYPETAWEVPWIHSLFENVAAVASGIEASLKKQGRSEKVVCVAGDGATFD
                     IGVLCISGAFERGHDITYICYDNEAYMNTGIQRSGATPYGASTTTSPAGTCSPGNLLP
                     KKDMPAILAAHGAPYVATASVAYPADFMKKVERAINTPGPCYIQVHTPCCTGWGFESG
                     ETLAMGKLAVDTGLWVNYEIVNGKVEKVKKVRRKPVEEYLAKQKRFRHLFKPARQDDV
                     IAHIQAVADANAERFGIDIKAKTPSG"
     gene            complement(14498..15073)
                     /locus_tag="PLV90_04625"
     CDS             complement(14498..15073)
                     /locus_tag="PLV90_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOF96432.1"
                     /translation="MIDIVAASFLIGLSGAASPGPMTASILGLGTRRPGPFVAGLIAG
                     HAVPEAAMVVAIAYGVRDIPYIDLIALIGSGVLIAFGVSQLLHAGDTLAASRETRAPV
                     AVGVACTLGNPYWWVWWLTFGVGFLALHPSFAAFYVGHIGADIAWLGLLTFAVSRGAN
                     VLGPHYKKVVQASGLAMVLFGLYFILTVFLP"
     gene            complement(15070..15606)
                     /locus_tag="PLV90_04630"
     CDS             complement(15070..15606)
                     /locus_tag="PLV90_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5803 family protein"
                     /protein_id="HOF96433.1"
                     /translation="MAAKEATFRVLPGGTTYEASVQVTGTGYGFWGPGLMGERVPLQV
                     EDLKVLGPAGPVDYREAGRGAIAFPEGNYTITYQVPVRDNHLVAIFDTPYAVTVALPE
                     GLDVKNPLIGMVSPGGAVSPGPNETMEITWDRTDAVEVRFYAPEREILLTTFGTIWLS
                     VAIVLLLPYFISWRRDGG"
     gene            complement(15627..>15812)
                     /locus_tag="PLV90_04635"
     CDS             complement(15627..>15812)
                     /locus_tag="PLV90_04635"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843557.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOF96434.1"
                     /translation="ASAASVAGIRKLAEQGLIDKDERIVCVVTGHLLKDPETVVRQCV
                     PPLEIDADLHSLLSALR"
BASE COUNT         3021 a         5216 c         4890 g         2685 t
ORIGIN      
        1 gtacgaactc gactctccgg aatccgaccc acgtattcga tcatgacggc gaaggggcaa
       61 gaacctacac ggtcaccctg accgtcacga acagcggcgg cagcgacgtg aagacaacgg
      121 cgatcaccgt tgttgcaccc cctcaaaaag aagacgtgac cgacagggtc gaccagacca
      181 atgcgacggc aaccgaagat atttcagtaa cgtatcaagg cctcacgctg gagatcccga
      241 agggtcatga cgcgaagcag gccgacggca gcccaataac ggaattcagc gtcggggttg
      301 ccccagatct cgaagagccc cctgcgggga ccatcgagat cggaggcaaa gcattcaagc
      361 tcgggcccga gggtgcgaag ttcaacccga agatcccgat caccatcacc ttcacccagg
      421 acgaatggga ggagctgttc ggtgacggga gaaccacaaa gctccagcgg tacgatggtc
      481 aaaactggat cgaacttgag aaccagacaa gggacgacac cacgtttact ataacggggt
      541 atacggattc cttcagtgta tttgcacccg tcactacgac cacgaccccg gagccgacat
      601 cgacacccac gcagccccgg acctccggca gcggaagcag cacggcaacg gtcacctcga
      661 ccggcacggc caaactcctc accgcctcgt ggggcggcgt gctccggccc tacctcgtct
      721 acgtcgacgg gaagttcgcg cacctctccc tggagaccgg ggttacggcg ctcgacgacg
      781 cgggaagccc gctctccgag atcgggatcg cccccgtctc gaccctcccc ggcgcgtctt
      841 ctctcgcctt ctcaggctac gcggtcgact gctcccccgc cggcgccacc ttctctccgg
      901 cgatcgacct tgtcttcacc ttcaccgggg atgagtgggc cgccctgctc gacagagcgg
      961 acggcgattc ggcccggctt gccgtgcagg gctacgacga tgcgaccggc acgtggaccg
     1021 cctgcccgac gaacgtcgat gccgacgccc gaacggtgac cgcgtcggtc agccacttca
     1081 gcacctacgt cctcttcatc ggaacggcga ccaaagtgcc ggtcacgacc ggaccgaccc
     1141 cgacggtgcc cgccgccgcc gccacgaccg cacctgttcc cccggcggag accggcgaga
     1201 gcccgctcgt ctggatcgcc ctggcggtcg tcatcgttgt ggcgctcgcc gggtacctcc
     1261 tctggaagag gcgctgaatg gggcctctac cctttttccg gcgagggaga tcggcgaaga
     1321 atatcctgca tctcacgcat gcgaatgtaa caacgtatcc gatggagtat cttccggcac
     1381 aaacgggatg atcttatgac gagaccgaaa agatggacaa tcggaatgcc agtgtttatt
     1441 ctggttgtcg cactgctcgt ggcaccggca ctggcagcgg gcaccaccga cgtgcgcatc
     1501 gcaaagtacg cagtcgacgg gacgaccgtc ttaaacgaga cgaccgtcga ctaccactgg
     1561 ctcaaagcga accttccggt ccagggggac ggcgtcaccc gttactacca ccagggcccc
     1621 gtcttcgaag ggcactggga ggatgcccac cccgacgagg aatacgacgg ctggaacccg
     1681 gccgaggacg tcctgatgag catgctggtc aaggccgacc tcggggcggt gatggggacg
     1741 gacatcgccg acatctgcga acttgtcggc ggcgccgcgg agggcgacga gatcgccgtc
     1801 cgcagccggg acgggtggca gagcaagcgc tacccctacg agtacatcta cgatcccgac
     1861 ccgcgccagg gcccggcggt cctctgctgg ttcagcggca actcatcagg ccccgacgcc
     1921 caggaggcgc agggccaggg ctacccggac accgggtatg ccgtcggcat gcgcatgatc
     1981 ttctttgccg acgacaccac gaacccctgg ggctggcaca tcttcgggaa caacgacatg
     2041 aaggagtgct gggacgagga gtactggaac aagggcgggg attactggtc tgccgccggc
     2101 acctccggca agtgggtcaa cgaggtccgg gtctacagcc aggaggagcc ggaaccgccg
     2161 accccagact tcggcgccaa cgtgacctcg ggcacggtcc cgctcgccgt ggcgtttacc
     2221 gatcggagta ccggggttcc gaccccgacc tcctggttct gggacttcgg cgacgacacg
     2281 acctccgacg agcagaaccc cacccacacc tacaccaacc ccggcaccta cacggtcacc
     2341 ctcgaggtca cgaacgtggc gggaagcgac acggcggaac tctcgattac ggcgacgtcc
     2401 ggcagcgacg gcggcgatac cgcggacacc gaatcccccg gctccgataa ccgcaccttc
     2461 ctcttcgagg tgccgggcat cgacgagatc gtcgtcttcg ccgcggagat cccgcagaac
     2521 ctgaaccttt cctgtgaaga aatcctgcta cccgacggga tccccgagcc gcccggcgcg
     2581 gtctaccgct actacaacat cacctcaagg aacgggaccg tcgccgtcga cagggccgtt
     2641 atccgtttca acgttccgga tgcctggttg caggagaact ggatcgtggg cgacgatctc
     2701 accctgtacc gctacaacga aacctgggcc ccccttgaga cacagtggat cggtaccgag
     2761 aacgagacca acacctatga ggccgtctct cccggcctct ccctctttgc ggtatccggg
     2821 atacagatca aacctgccac cgccgccacc accgcccctg cccctacccc gactcccctc
     2881 gcgaccaccc ccaccgggac ggcgacgccg gcggtatcgg gtaccccggc gccggccggc
     2941 aagaccccgg aaaatccgct tgtctcgatc tttgggtata caggggtttc caccgtcggg
     3001 ggtctgcccg gtcggctgct cggcgccgtc caggcgttcc tcgggttgtt cgggctcctt
     3061 cccgctgcgg cggacccggt gacggagccc gaaccggttc cgaccgcgcc cacccccgcc
     3121 ccggttcagg ctgcgcctga acccacgccc acgcccgtgg acctcaccgc gatgcacttt
     3181 gggctttccg tactctccga tccgccgggt gcgctcatcg acctcgacgg tgagtatacc
     3241 ggcaggacaa ccccggccgc cttcgcgtcg ctccccggcg gtacccatac cgtccgggtg
     3301 cgtctggacg gcgccgagcc ggtggagcgg gagatccggc ttgcgcacga cgacgaggtc
     3361 gtgatcgaga tcccggtatc cgcctcagtc cgctcggcct ccccgggcct gctccgcggc
     3421 cgggagcaga accactacgg cggcgtctac gtcgactcgt tcccggcggg ggcggagatc
     3481 ttcgtcgacg gtcgcaaggt gaaccggaag acccccgccg tcatctacgg gctcagggag
     3541 gggctgcaca ctatcagggt acaggaagag gcggttgcgt tcagctccgg gggagagcag
     3601 gtctggatcg agagaaacac cgtggcacgg atgatgttta ccggcggcaa cgaggaattg
     3661 aagcggtcga tcgcattcga gtcggaagag tacgcaaagc agccgttctc ggtcaacggc
     3721 aggtacctgg gcgaccggtt cccgaagacc gtcgaggtca gcgggatcgg ggaggcgtat
     3781 cttgccgtcc gcgacggcaa gagttaccgg acgtaccgga tacccaccgc tgccgggtcg
     3841 ggcgataccg tgaacgccat attctccgcc gcgccctgca gcattctcgt gacgtcgacg
     3901 ccggcgggag cggcgatcgc gatcgacggg ttccagaccg ggtttgccac gccgtatctc
     3961 gtcgacaacg tttccggcgg aaaacacctg gtctcggtat caaagccggg gtatattccg
     4021 gaggaacggg agatcctgct cacggtcggc tccggcgacg ccacggcgaa gttcatcctc
     4081 gaaccctata cctacggatc gctctcggta tcgagcacgc cagcggacgc caggatctac
     4141 ctcttcggcc gggacaccgg cgaaaagacg ccccacacct tccactatct tcccatcggc
     4201 agttacccgg tgaaagtgac ggaagtgaac aggacagtga ccgaagatgt gctgatcgca
     4261 ccgtaccaga tcgcccggat ggaggttccg gaggtataac accccgggtc ttttgcacga
     4321 tgcatcgcgg attcgcttca ccagaagtta aataccacta aattaaatta accaggttta
     4381 tattttttat aagtaaatag aaagttattt actcggaatc gacccaatca acgtatatta
     4441 taaggcgtat ccagaatcgc cgaaccgtga gttgagccaa atgatacgac ccgatcagat
     4501 aataatcgtc ttcctggtgc tgatgctcgc cctgctgccg gcaccggcgc tggctgcagg
     4561 ggcggggact accgagctcc gcatcgtgaa atacgcaaac gacaggacga ccgtcttaaa
     4621 cgagacgacc gtcgactacc gctggctgga ggcgaacctg cccgtccagg gtgacggcgt
     4681 cacccggtac taccaccagg gccccgtctt tgagggtatc tgggatgaag tccaccccga
     4741 tgagccctac gacggctgga acccgggcga ggatgttctg atgagcatgc tggtcaaggc
     4801 cgacttcgga gcggtcaagg ggaccgacct cgccgacatc tgcgactccg tcggcggtgc
     4861 ctcggagggc gacgagatca atctccacag ccgggacgga ttctcaaaga ccttccccta
     4921 ctcgatcgtc tacgagcccg actcgcggca gggcccggcg gtcctctgct ggtacagcgg
     4981 caacggctcg ggctccgacg cgcaggaggc gcagggccag ggctacccgg acaccggcta
     5041 cacggtcggg atgcgcctga tcttctttgc cgacgactcc acgaacccct ggggctggca
     5101 tatcttcggg aacaacgaca tgcgggagtg ctgggggaag gcctactgga actacggcgg
     5161 atcctatccc tccgctgccg gcgcctcccc caagtgggtc gacgaagtcc ggatctacag
     5221 caaggaagac ccggccgcaa accattccgc cgccacacgc ggaccggtgg aaggcggtga
     5281 gactcctgcg tcgctctcgc ccatcctcgg aagcgttgcg gcactcctcg ccgtactcgg
     5341 cgtatcgttc cggtacctcc ggcatgagcc ggacgacgat caatcacggt gatgaacatg
     5401 acgacacgtt gtctctcaaa aattctcgcg tgctgcgtcc tcctcctcgt ctgctgcatg
     5461 ccggcgacgg caggcatacc gggggatacg gacggagaca ccaatatctc ggcagacgaa
     5521 ctcgcgacgg cgatcctggg ctacctgggc ggtaccgggg ccctcactgc ggaagagctc
     5581 tcgctcgccg cccacaacca cctgcatcaa ccttacgggc agctggtcgc cgccgtcctg
     5641 ggggagactt acctcatccc gtcgacgagt tatatcgacc agaaaggggt tcccgccgac
     5701 tccctcatcc acgaagggct cgtgaccaaa ctgcgcccgc aggacgacgg ctccggagga
     5761 tacctcggct ggcttgcgga gaggtgggag atgtccgacg acgccatgtc ctggacgttt
     5821 tacctggtga agaacgccac gtggcacgac ggcaccccct tcacctcggc agacgtgcag
     5881 ttcacctacg agtacttccg tgaccacggg gatgccgacg ctccggccgg ctcgttcctg
     5941 gtgaactccg cgatcctctc aaacgtcgca tcggtcgaat gccccgaccc ctacaccgtc
     6001 gtcttccgca tgaagtcctg cgcaccgctc ttccccgaga ccctcgcgac cggtccgggg
     6061 atcggggtgt ttcccgcgca tatctggaag gatatcgatc taccctcgac atacgtcgac
     6121 tcaaagcaga tcggcatcgg ccccttcgcc tatacgtcga cgatcccggg caccctctcg
     6181 aaggtgaccg cctacgaggg ctaccacggt gagcaacctt atgtgcgcga gatcgtgatc
     6241 cggaactacg aagacgaaga cgcggcgatc ctcgccctcc ggcgagggga tatcgacttc
     6301 atcagcgggg agttcgggct ctccccgagg aaggccgagg ccctcaaggg cgcccccggc
     6361 atcgggatca cccagatccc ctcgggcgga caggccttcg aggtggcgtt cacgtccgac
     6421 ttctatccgg caaacatcac cgcattccgc cgcgccctga gtcacgccgt gaaccgggaa
     6481 cgggtctgct ggctggtcgg cgatggagcc cgcccgacgg agacggcctt cctcctcccg
     6541 gccctggccg gcgaccagat caaccccgcc acgaacggcc ggttctccta cgacatcgag
     6601 gaggcgaaga ggcagctcgt cgcggccggt ttcaacctga ccgaggcaaa cgggagaccg
     6661 gtcctcctcg atcctgatgg aaaccgtgtc gagatctcca ttcccctcgc cggcaaagcg
     6721 tccacgaacg ccgtcgacca gaagatcgtg gccgtgctca aggaggactg ggaggggaaa
     6781 ctcggcatac ggatcgtcga gaccccgcag gtggacgact cgatctacct ggactggatc
     6841 gcaaggacag ccgttcactt cgacggcatg cccggtcgct ggcacgaaga catcgaccgc
     6901 ctggacaact tccagaggag cccgctcggc gagaactact accacttcga caacgagacc
     6961 ttcaacaggc tgatcaccga actgaagagc acggccgatc ccgcagagcg aaaggagatc
     7021 ggcttcaggc tccaggagat cctcgtcgaa gaggtgccct gcatcccggt cttctcccag
     7081 gacgctttca tggcctaccg gtcggatcgg ttcgtcgggt gggacgcccc cctgctcggc
     7141 ggcggcggga acatacgcct ctttgccgag atcaggccgg cgaacgccga ggtgggactg
     7201 agggcgtgat gtctgccggg tggccctgtc ctcctctttt gaactccggg tctcgatcat
     7261 gaacggcata tacctcctca aacggctggc aacctcgttt gcagccttct gcatcgccgt
     7321 gaccatcatg ttcgtcctcc tccgcctcat gcccggcgac tacatcacca tgtacctgga
     7381 gtcgatctac cggtacgccc ctccggacgt cgtcgagaca ttcaaccgtg tctacggcct
     7441 ggatagacca atcctggaac aatatttcac ctacctcctg gacgccttcc gggggaactg
     7501 gggatactcc ttcatctaca aggtcccggt cctcaccgtc ataggcgaga aacttgcctg
     7561 gacgctgatc atcctcctcc cggcaacgat cctcgccacc cttgccggca tcggcatagg
     7621 cgcgtactcg ggctggaggc gcggatcgaa ggccgacgtc gggctcctga acgggatgat
     7681 cttcatccgg gcgatcccct cctactggct cgccctcatg attctcatca tcttcgccta
     7741 ctacctcaat ctcttcccgc tgggggggta tgtcgacacc agcgctctcg cgaccgggat
     7801 cgacccgttc gacgtggcaa accacgctgt gctcccgatc ctgacgcttt tcctcttctc
     7861 ggtgaccgga acctactacc tgatgaggaa ctccatgctc atgacggccg gggaagacta
     7921 catcaccacc gccagggcaa aagggctcga cgagctgacg atcctcagga agcacgcctt
     7981 aaagaacgcc atgctcccga tggtgacgct gatcacgctc gagtgtgcgg gcatgatcac
     8041 cgggagcatc ttcgtcgaga cgatcttctc ctggcccggc ctcgggatgc tcacctacaa
     8101 ggcccttgcc gcccgcgacc tccccctgct ccagggaatc ctgctgatcg acacgctctt
     8161 cgtcatcgcg gcgaacatcg ccgccgacct gatctacccc ctcgtcgatc cgcgcgtgca
     8221 ggtgggtgac gatgcctagg aagaaggtca tccttgccgc aagcttcggc atcgtcttcg
     8281 cgtactgcgc ggtcgccctc ctggccccgg tgatcgcgcc ctacgaccct cacgcgatca
     8341 cgggcgcgcc gctctccccc cccgacaaag accacctgct cgggacgaac gacctcgccc
     8401 aggacgtctt cagcgggctc atatggggca cgcgggcgac gctgctcctc ggtttcatcg
     8461 gagccgccat cgcggtcgcc atcggcaccg ccgccggcat cgtgtcgggg tactacggcg
     8521 gcctgatcga cgaagccgtc atgcgcctcc tcgacgtggc gatgaccatc cctatcttcc
     8581 cgctcctgct catcctcacg acgttcttca gaccggggat ctacctgatc gcggtcctta
     8641 tgggccttct cggcagtttc cagggcgtgc gggtgatccg gtcgcaggtc ctctccctgg
     8701 cggtcgccga cttcgtcgtc gggacacgag cgctcggcgc cggcgacggc tacatcatgg
     8761 cccggcatat ccttccgggc gtcctccccc tggcaggcgt caagttcgtc acctccgccc
     8821 agcacttcat gctgatcggg atcggcctcg gattcctcgg catcacggac ccgagcgtcg
     8881 tggactgggg ccagatgatc aaccgggcga gccagaacgg ggcgatggtc ctcggcctct
     8941 ggtggtggct gattccgccg ggccttgcgg tgacgctcat ctcccttgcc ctcgcgtggc
     9001 tggggtacgc ctgcgaggac gagatcaatc cgaggctccg ggtgatgcgg atatgacgaa
     9061 cgaagcgctc cttgaagtcc gggggctcaa agtagggttc cccaccccgg acgggctggt
     9121 cagggcctcc gataccgtgg atctcgagat ctacccgcgg gagaccctcg gcctcatcgg
     9181 cgagaccggg tcggggaaga ccgtgctcgg catggcgatc cttcgcctcc tccagcccaa
     9241 cgccgtcatc gagggggaga tccgctaccg cgaccacgac cttctcaccc ttccggagga
     9301 cgagatgcgg aaggtgcggg ggcgggagat cgggatcgtc ctccagaact cggggacctc
     9361 cctcaacccg gtgtacacgg tcgggaccca gattgcagag gcggtcaccc tccaccaggg
     9421 gttgagcggg cacgacgccc ggcagcgggc ggtggaactg ctcgacgccg tcgggatacc
     9481 cgatccggtc aggatggccg gggagtatcc ccaccggttc agcgggggga tgcgggagcg
     9541 ggcgctcatc gccacggcgc tcgcttgccg tccgaccctc ctcatcgcgg acgaacccac
     9601 gaaaggcctg gacgccgtca ccaagcgggg gatcgtcgac ctcttctcgg agatcgaccg
     9661 ggaccgctcc gtcctcttca tcacccacga catcgagacc gtggaagacc tcgccgcccg
     9721 ggtcgcggtg atgtatgcag gcgagatcgt cgaggtaggc ggggtaagag aggtctttgg
     9781 agacccggcc cacccgtaca cccggggcct cctcgcctcg ctccctgaac ggggaatgcg
     9841 accgatcccg ggctcggccc cttcgctcat caccctgccg cccggatgcc ggttctcacc
     9901 ccggtgcgga tgcgcgatcg gaggctgcaa cagagaacac cccggacttg tcgaggtcgg
     9961 gcccgggcat tatgcacggt gcatgctcta tgattgaggt tactgatctc gagaccaact
    10021 tctcgacggg cttcatccac cggagggtgg tccgggcggt ggacggggta tcgttttgga
    10081 tcgaggaggg cgagaccctc gggctcgtcg gtgagagcgg gtgcggaaag acgacgctcg
    10141 gccgttcgat cctccgcctc atcgagccgg acaggggccg gatcgtggtc gacgggaccg
    10201 acgtcaccgc cctctcccgg agcgaactca ggcgcttccg gccacggatg cagatgatct
    10261 tccaggacgc ctacgcctcg ctcaacccgc ggatgcgggt cagcgagagc gttgccgagc
    10321 ccctgaagat ccaccggatg gggagcaggg ccgatatcga ccgaagggtg cgggaactga
    10381 tcgatgaggt ggggttgaac gaggagcacc tgaaccgccg gccttacgaa ctctccggcg
    10441 gccagaacca gcgggtggcc cttgcccgcg tcctcgcgct caaaccgaaa tttctcgtgg
    10501 ccgacgagcc cacgtcggcc ctggacgtct ccgtccaggc ccagatcctc aacctcatcc
    10561 gcgacctcgg ccgcgactac cgcatgacct gcctgtatat atcgcacgac ctcggcgtca
    10621 cccgggcgat gagcagccgc gtcgccgtca tgctggaagg aaggatcgtg gagatcggca
    10681 ggaccgccga cgtcttcgat cgcccctgcc acccctacac ccagaggctc gtcgatgccg
    10741 cgcttgcggg acgagcccgc gcacccccgg aaaggccgcc cctccccgcg gcgccgacga
    10801 tggtcgaggt gcggtcgggc cactgggtgg aagagacggt gtaatgcatg atcggcgggt
    10861 caccgctcct ttcgtgcggc gcacgcgccg gcagcgacgg ccgcagctgc aagagccggg
    10921 acgaccgccc agggcgcgcc tcccgcactg gtcgggccgg gtgcctgccc ggtacccgcg
    10981 ggcgctgcgc cggctgccgc tgccccggac ccgacagtca ccgcagtcgg ggagatcgct
    11041 ccttcggtct tgtcgacggc gtcaacccac gttccggaaa acgtccagga gccggcggcc
    11101 tttgcctgga cccggtaggt gatcttgcgt tcatcgatca ccgcaaacgt caatctctgc
    11161 ccggatacgc tgtaacgatc ctccggaaag gatgtctcga ggaagacaaa accctccggt
    11221 atcgtctcca cgacaccgcc gacgccgaga tcgtcgaccg ccaacgtcac cagaacctcg
    11281 ccgccgaccg ggaccgaaga acggtcgatc tcacgggaaa cagcggcatc gcttgcccag
    11341 gcgggcacga cgcagaatga ggcaaggatg aggatgcagc agatcggcac caggcccctg
    11401 gataccctca caggattgtt taccatagca atcaccaatt gttaacattt gtcttttgtg
    11461 aatgatttat atttaactgg attgattctc atcggtgccg atagattgcg gcaggttttt
    11521 accgcgggtt cgctcccggg gattgagcgg gagatcccgc gccctcctgg gagcgcggtg
    11581 ttgcagggcc ggccgcaggc aaagtaccgg tgtatttata ttattaatca ttaagattgt
    11641 gaaggggttt ttgtttgaaa gaattacgca ttcatggcag gggtggccag ggctccgtga
    11701 ccgccgccga gctcatcgct ttcgcggcgt ttgagggtgg cgcgtatgcc caggcattcc
    11761 cggccttcgg cgtggagcgg cggggcgccc cggtgcaggc gttcgttcgc ttcagtgatg
    11821 caaagatccg gctccggagc cagatatacg agcccgacta catcattgtc caggactcga
    11881 ccctgatcgg tgatgtgaac gtctttatgg gcatgagagc aggcggtatt gccatcgtca
    11941 acaccgagaa gcctgacttc gactcccggg tcccggaggg cgtgaaggtc tataccgtcg
    12001 atgcgaccac catcgcgctc gaggaactgg gcgtgcccat caccaacacg accctgatgg
    12061 gggcttttgc cgcagccacc ggcgagatcg ggcttgaacc gctgaaacat gcacttcaga
    12121 ggcgtttctc cgggagtatg gccgagaaga acatccgggc ggcagagcgt gcatacaacc
    12181 tgatcggagg tgctgcataa tggcgctccg agtcggctgc gctgcgcctc ccggaagagc
    12241 gcgcgataac aagaccggta cctggagggt cttcaaaccg gtcttcgatc cggagacatg
    12301 caaccggtgc ggcatgtgcg ccatggtctg cccggagggg tgcgtccgcg agacggaaga
    12361 gggcacgttc gagcccgacc tcgactactg caagggttgc gggatctgcg aagagatctg
    12421 cccgaagaaa tcgatccgga tggagaagga ggagaagtaa tgctggagtt tatggaaggg
    12481 tcgcatgcgg tggccgagat cgtgaagcgc tgccgcccgc aggtgatctc agcctacccg
    12541 atcacgccgc agacgcacat cgtcgaggac ctcgcccaga tggtggcgaa ctgcgaactc
    12601 gacgccgagt acataacggt cgagagcgag ttctcggccc tctccgcctg cctcggcgcg
    12661 agcgcggcgg gctcccgggt ctactcggca acgacctccc agggccttgc cctgatgttc
    12721 gaggtctgtt tcaacgtggc agggatgcgc cagccgatcg tgatgacgat cgcgaaccgc
    12781 tcgctcggcg cgccgctctc gatctggaac gaccagcagg actcgatatc cctgcgcgac
    12841 tccgggtggc tgcagttcta cgcggaggat aaccaggaga ccgcggacct ccacatgatc
    12901 gcctacaagg tctgcgagga ccacgacatc ctcctgccgg cgttcgtctg cttcgatggg
    12961 ttcatcctca cgcacaccta cgaaccggtc tccctcccca cccaggagga ggtggatgcc
    13021 tacctgcctg cctttacgcc ctaccagcgt ctggatgccg cgaacccgct gagtatgggt
    13081 atgtacgcga cacccgagta ttacatggag ttccgctacg agatcgaccg ggcgatgcag
    13141 cgcgcaaggc agtcgttcat caaggccggg cgcgagttcg gcgaacagtt cggccgggac
    13201 tactccggcc tcgtcgaggg ttaccgcctt gaggacgccg ataccgcgct catcgcgatg
    13261 ggctccatct gcggcacggt gaaggatgcc atcgacgaga tgcgcgatgc gggcaaaaag
    13321 gtcgggctct tgaagatccg gacctatcgt ccgttccccg ctccggagat cgtggatgcc
    13381 ttgaagggtg tctcgacggt ggcggtgctc gacaagaaca tatcgctcgg gaacggcggg
    13441 gcggtcggca ccgaggtgaa gtcggcgctc tacggctccg gcgtgaccgt gtatgactac
    13501 atcatcgccc tcggcgggcg cgatgtgagg aagagggata tcgccgcggt cgtcgatctc
    13561 gcggagaaag ggaaaggaga tatgttttac ggattacgga cggaggtgct ctgaatggct
    13621 gaaaaagagc agggatgcga actcttctcg gccgggcacc gggcgtgcgg aggatgcgga
    13681 cctgcgcttg cggcccggct cctcctcaag gcgaccggga agaacgtcat catcgccgcc
    13741 tcgacgggat gcatggaggt cttctccaca ccctaccccg agaccgcctg ggaagttccc
    13801 tggatccact cgctcttcga gaacgttgcg gcggttgcat cgggcatcga ggcgtcgctg
    13861 aagaagcagg ggcgcagcga aaaggttgtc tgcgtcgccg gcgacggagc cacgttcgat
    13921 atcggggtgc tctgcatcag cggcgccttc gagcggggcc acgacatcac ctacatctgc
    13981 tacgacaacg aggcctacat gaacaccggg atccagcggt cgggtgcgac gccctacggc
    14041 gcgagcacca cgacgagccc tgcgggcacg tgctcgccgg gaaacctgct cccgaagaag
    14101 gatatgcccg cgatccttgc cgcccacggt gcgccctacg tcgcgaccgc ctcggtcgcc
    14161 tacccggccg acttcatgaa gaaggtcgaa cgcgcgatca acacccccgg cccctgctac
    14221 atccaggtgc ataccccctg ctgcacgggg tggggttttg agtccggcga gacgcttgcg
    14281 atgggtaaac tcgccgtcga taccggcctc tgggtgaact acgagatagt caacggcaag
    14341 gtggagaagg taaagaaggt gaggcgcaaa ccggtcgagg agtacctcgc aaaacagaag
    14401 cgcttccgcc acctcttcaa gcccgcccgg caggacgacg tgatcgcgca tatccaggca
    14461 gtcgccgatg cgaacgccga acggttcggg atagacatca aggcaaaaac accgtcagga
    14521 taaagtacag cccgaagaga accatggcga gtccgctcgc ctgcaccact tttttataat
    14581 gagggccgag cacgtttgct ccccgtgaga ccgcgaatgt aaggagcccg agccaggcga
    14641 tatccgcgcc gatgtgtccg acgtagaacg cagcaaacga cgggtggagt gcgagaaacc
    14701 cgacgccgaa cgtgagccac cagacccacc agtaagggtt gccgagcgtg caggccaccc
    14761 cgacggcgac cggcgcacgg gtctctctgc tcgcggcgag ggtatcgccg gcgtggagga
    14821 gctggctgac gccgaacgct atgaggacgc ccgacccgat gagagcgatc agatcgatgt
    14881 acgggatgtc acgcacgccg taggctatgg ccacgaccat ggcggcctcc gggacagcgt
    14941 gccccgcgat aagccccgca acgaacggcc cgggtcgccg tgtaccgaga ccgaggatcg
    15001 aggcggtcat ggggccgggg gatgcagctc cggagaggcc gatcaggaac gatgcggcga
    15061 cgatatcgat catccgccgt ctctcctcca cgatatgaag taggggagga gcagaacgat
    15121 ggcgaccgag agccatatgg tgccgaatgt ggtgagcagg atctcgcgct cgggggcgta
    15181 gaaacggacc tccacggcat ctgtccggtc ccaggttatc tccatggtct cgttcggtcc
    15241 gggcgagacg gccccgccgg ggctgaccat accgatgagc ggattcttga cgtcgagccc
    15301 ctcgggaagg gcaacggtta cggcatacgg cgtatcgaag atcgcaacaa gatggttatc
    15361 cctcacggga acctggtagg tgatcgtgta gttcccctcc gggaaggcga tggcgcctct
    15421 ccccgcctcc cggtaatcga ccggacccgc ggggccaagc accttgagat cctcgacctg
    15481 gagcggaacc cgttccccca tcaggcccgg gccccagaat ccgtagcctg ttccggtaac
    15541 ctggaccgag gcctcatagg tggtgccgcc ggggagcacc cgaaaagtgg cctcttttgc
    15601 cgccaccgcc ggggtgcaga tcaggatcag cgcaaggcag atagcagcga gtgcaggtcc
    15661 gcgtcgatct cgaggggagg aacgcattgt cgtaccactg tctccggatc ttttaagaga
    15721 tgaccggtca ccacgcagac gatccgctca tccttatcga tcagaccctg ttcggcgagt
    15781 ttacgtatcc ccgcgaccga ggctgcggag gc
//
LOCUS       DAOSII010000040        15549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_3948_length_15549_cov_49.928359, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000040 DAOSII010000000
VERSION     DAOSII010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 15549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15549
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3948_length_15549_cov_49.928359"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..76)
                     /locus_tag="PLV90_04640"
     tRNA            complement(2..76)
                     /locus_tag="PLV90_04640"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39..41),aa:Arg,seq:tct)
     gene            complement(161..550)
                     /locus_tag="PLV90_04645"
     CDS             complement(161..550)
                     /locus_tag="PLV90_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF473 domain-containing protein"
                     /protein_id="HOF96435.1"
                     /translation="MKCAALTGISPSVIAELKTGRARTLELNSAHNIITLTEAAPGEC
                     IFMTSVNGEDLSPGDSGIMVDLLALTISMKRVEVVNPLYYEERERMSARVKVQFRGTT
                     IARGVEERKWGDPTMVEIIRSACYRAG"
     gene            complement(557..1270)
                     /locus_tag="PLV90_04650"
     CDS             complement(557..1270)
                     /locus_tag="PLV90_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome assembly chaperone family protein"
                     /protein_id="HOF96436.1"
                     /translation="MDDIKVISRALAGAEKTVLIGFPGSGLVGSIALQYLVEQMDFEQ
                     IGAITSKYFPPVALMTRGVINAPVRLYEKDSLAAIISDVPVHPMICYEVANGIMDWLT
                     QFGIREIATIAGIITNEPDKRVFGVATTSTVLQRIEEQTIILPMGSISGIAASILTEC
                     KTRGIPGIGLLGETVNTPDPRSSAATIEVLNKIYDLDLDVQPLLEQAVEIEAAMAQIA
                     EQVQKTEAQPRREQLPMYG"
     gene            1330..1656
                     /locus_tag="PLV90_04655"
     CDS             1330..1656
                     /locus_tag="PLV90_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5611 family protein"
                     /protein_id="HOF96437.1"
                     /translation="MQEYQIKRGYTKDLAKSMVQGLRDQFGLEPEVAADGHYIVAYGA
                     LHRLEVWSGAGGKTLVVDTEANRDAGDEAIIDTNRRFRNYLQQVTGYTAKERAKKAQQ
                     ALKGSE"
     gene            complement(1708..2097)
                     /locus_tag="PLV90_04660"
     CDS             complement(1708..2097)
                     /locus_tag="PLV90_04660"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HOF96438.1"
                     /translation="MQGVVSAVEAIMMASSSEFVEYVAGQLAGAGNITYRKMFGEYGI
                     YCDGKFFACACDNQFLVKITEAGKKFMPDGRTASPYPGAKPCFLIDDLDDREFLRELT
                     AITCAALPAPRPKKRRAGTAARRQGEE"
     gene            complement(2073..2438)
                     /locus_tag="PLV90_04665"
     CDS             complement(2073..2438)
                     /locus_tag="PLV90_04665"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOF96439.1"
                     /translation="MPMLLFDGEFVGTREQDRRLSGLFAAAEYRQERMRVIESRCGIL
                     CSGCGYRASMGCGGCMAIKRPFWGEICPLKTCCEARGLENCGRCGEFPCEVLVQFAFD
                     ERQGDGGRRIEQCRAWSLQ"
     gene            complement(2559..5324)
                     /gene="leuS"
                     /locus_tag="PLV90_04670"
     CDS             complement(2559..5324)
                     /gene="leuS"
                     /locus_tag="PLV90_04670"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOF96440.1"
                     /translation="MQSNERECIARWEHAFEADPEREKEKYYLTVAYPYPSGAMHVGH
                     GRTYIVPDILARYQRMAGKQVLFPMAFHVTGTPVIGISKRIANGDEKTVGLYRDLYRV
                     PQDILDRFINPMEIVRYFSEEYRRVMQKSGLSIDWRRRFITVDPPYSKFIEWQYKHLR
                     EDEHVVRGAHPVKYCPQCENPVGDHDLLEGDKAEIIRFTLVVFRWGNARVPCATLRPE
                     TIYGVTNLWVNPAVTYVRAMIDGKEWIVSSEAAAKLSLQDHTVTVTEKIPGTALVDQT
                     VTHPLSGDVPVLPATFVDPDMGTGIVMSVPAHAPFDYIALRDLQQQGQYTSVEPIPLI
                     AVEGYGDIPAKDAVERAGIHDQNDPGMEALTQEVYSAEFSRGKVFEKYGGKPVREARD
                     DVAALMMERYDSVPMYDFDNRLVICRCGGRVFVKILHDQWFLEYSDPCWKEQVKTQLE
                     EMALVPPEVRAEFIRTVDWLKDWACTRRVGLGTKLPWDPTWIIEPLSDSTIYMAYYTI
                     AHHLKAIPPEKLTPAVFDYIFFGMGSPETVDRETLDAIRSEFLYWYPYDYRFSAKDLI
                     SNHLTFQLFHHRAIFPSYLQPKGMVVFGMGLLNGAKMSSSKGNVFLLEDALEEFGADT
                     VRMFLVGSAEPWQDFDWRNELVSSTKRQIERFWNTVSEAMQAEGATPIDAWLASRLER
                     RIEAATAALDSFQTRQALQEAFFGVESDLKWYRRRLPEGAGGGAVLQDLCRTWVRLLA
                     PFIPFTCEALWSDLGESGMVSFAPWPETDESRINPGVELAEELLARTVEDIESIVKLI
                     PMEPASISLFVAPAWKYEAFRIIAGSADKTRVMREIMQNESMRRRGREATDAAKQITK
                     LVLKLPPDIVKQLAASPLDERAVLEGARAFLEHEFGVPVTVKDAGESNHPKAAAAMPF
                     KPAIMIE"
     gene            complement(5382..7277)
                     /locus_tag="PLV90_04675"
     CDS             complement(5382..7277)
                     /locus_tag="PLV90_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PINc/VapC family ATPase"
                     /protein_id="HOF96441.1"
                     /translation="MKIVPDTSVVIDGRITSLIKTGEYKGATIIIPEAVIAELEAQAN
                     QGREIGFSGLTELQELFRMSVDGIIELQFSGERPSLDQVKLSSGGEIDALIRNVAIVY
                     GARFITSDLVQAEVAKAKGLDVVYLRPQIGDFSPLSIDQFFDEETIAITLKERALPVA
                     KVGGLRDTRLVTLRDTPMSEYELRSMAQELLERAKRDPDGFIELEKRGITVVQIGSLR
                     ISIARRPFADGMEITVVRPLVDLSLDDYDMAPEIRQRILGNRRGVLIAGPPGAGKTTL
                     AQGLATFLADHDFIVKTMEAPRDLQVPDHITQYMALEGSMANTAEALLLVRPDYVIFD
                     EIRKNEDFNVYADMRLAGMGMVGVVHAMEVHDCLQRFCDRVDYSILPQIINTVIFVVQ
                     GAITKMYDLELTIKAPEGMPGETHVRPVIVVRDHARQETEIEIFRYEGETLVMPLTRV
                     AVEPPAVLEPAPVVAAAAAAPEPGENVAWKVAEKEVQREIGRYTSGPVDVRIISDNKA
                     VVYIEDKDVPAAIGKGGKNVSAIVNKLGIGIDIRPHSELEAKKPVEEEMQLAGGIRIR
                     IDKKQLTIISPENKGKIVDVFAGKEYLFTATVNEEGDILLAKNSTIAQEMIKRYNEGE
                     TIRLRPV"
     gene            complement(7301..7669)
                     /gene="hisI"
                     /locus_tag="PLV90_04680"
     CDS             complement(7301..7669)
                     /gene="hisI"
                     /locus_tag="PLV90_04680"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOF96442.1"
                     /translation="MEIQFNDNGLVPVIVQERRTRDVLMLAYANALALELTRTTGYAH
                     YYSRSRQKLWKKGEESGHVQRVCRILVDCDADAVLYEVEQVGAACHTGHASCFYRTAD
                     GEEIAGMVFDPKKVYANKGE"
     gene            complement(7676..8470)
                     /locus_tag="PLV90_04685"
     CDS             complement(7676..8470)
                     /locus_tag="PLV90_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HOF96443.1"
                     /translation="MILASIPLMIAAAAIGVTLIYASILDARERRVPHRTWRPALAIA
                     VPAAAIVYGLATLADWQAAAGYLVLVVVFCGFFYLFAARNLFGGADAYALIIITACIP
                     FFPVEPYFGIPPLGFFPFTVLTNAVLINIAAPVAILAKNLVEGNRAPFPCLFLGFPVD
                     GKTIQNEFGFVMEDIEEEDGRLVRRFVGFAGSLGRMVRGEQRIYTKDLRLHPEDYERE
                     LALYRKAGRVWISYGIPFIIPIAAGFLTALFIGDILFVIMATFAGV"
     gene            8822..9442
                     /locus_tag="PLV90_04690"
     CDS             8822..9442
                     /locus_tag="PLV90_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96444.1"
                     /translation="MNEILLQSTYEGCFTLDELLHHGSVHSLTGIGASKEQTQAFFLV
                     LLGGEPDGAIFIDEKGTLFGDTAVLHLTGQEEFNLHRVAPPIADALVARCRVFEKSHL
                     KKSGRLEIPTIGAPPRQRVGVLCLTVQDNRVPLAGAHVSIRKGKLVITGDVTDSEGRV
                     CFRLLAGRYMCVVTDRTGERTRCIIEFHEPQVETFVDIGGTEDEST"
     gene            9570..11774
                     /locus_tag="PLV90_04695"
     CDS             9570..11774
                     /locus_tag="PLV90_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOF96445.1"
                     /translation="MEIRGETDPDPGETDDTAAFPPVAAPESEDTRILARPGHTGDRR
                     AADREDPIRALLDRIERRRMGEEAASPPSPVAEETGTTPAPFGILGRMRGRKDAGAPP
                     LDEPDPVDDAGAPPLDESDPVDDAGVISVEELGNLADLILPKSATFTVDELSINHHEH
                     RFDFVDNARVVSEFDDLFARAFSTTTLTAAEIAVPAEPEVQSRFGFLKKFYPTAGEII
                     EEYNLTLHGPLVDLSLRPTPGVEEIELYPVNEPYAYVRVTYDSTTHEYTYHVLEPVLT
                     PGEQELLSEIKERLFETLNIATRDLTRDAAKKALRGAANTIVADYGIRLPPPGREKIL
                     YHVEKEFLGDGLIDPVMHDKYIEDISCDGVGSPIFVYHTTYESMRTSLVYRNAGELDS
                     FVTKLAQRAGKYISIAEPMLDATMTDGSRIQMTLGSEVTAHGSTFTIRKFRDEPITPT
                     DLIEWHTFSPLGIAFLWLAVENAKSCIFAGGTASGKTTTLNAISLFIPPLAKIVTLED
                     TRELKLPHPNWIPSITRDSFSQDGRGEIDMYELLRAALRQRPEYILVGEVRGREALTL
                     FQAMSTGHVTYATMHADSVASAVHRLENPPIDVPRNMLSALSLVSIQVQARVGGQRIR
                     RNKQLIEILDIDPRTNELITNEVFRWHPATDEIRYSGKSYILEQIMEDRGWSEERMRE
                     ELKRRQEVLEWMRIKKIRHFRDVSKILVSYFRDPESVIERVRTDLYGEGGPA"
     gene            11771..12694
                     /locus_tag="PLV90_04700"
     CDS             11771..12694
                     /locus_tag="PLV90_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOF96446.1"
                     /translation="MNGYERFCFNLLGRNLKAKRGEYVSLRNDLTGARMNTPFEAYLA
                     TAYVSSIAVGLVAAALIGILTYALRVPEMISYHGAVPEVFYALNAYKLLIGTVVITLL
                     SLLIFGGITYLIFLLYPGIRAGERRRNIDATLPYAINYVTAMSSAGITPDEVFRLLGQ
                     SRIYGESAVEARYVARETDFFGKDLLDALRSVSQATPSERMREFLQGAVASISSGSNL
                     TEYFRAKARQYTLENNQQQKTFLETLGLIAESYVTAMVAGMLFLIILQSVMTILSGDA
                     NPFFLYIIIYLIVPFGSIMFVILISSMTPEV"
     gene            12696..13730
                     /locus_tag="PLV90_04705"
     CDS             12696..13730
                     /locus_tag="PLV90_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOF96447.1"
                     /translation="MAFKEIIDDFLNRNQPRGPAAEESFADEPFGEREIFSRIENRRK
                     YQEGFYRFIRHPLRVMIEDPVTVLFVSVPVALFLLIGGSASLVMSYGPGILLTTTMVD
                     DIFVFALLIAIVPLAALDLKEALRVSSIEASLPNFFRDVAGMNDSGMTLPHAIHIVSE
                     GEYGALTPHIRKLDTEMSWGVPFVEAIRRFGKVVNTPLAERSVDLIAHASSAGGDVSE
                     VLRAAAHDAYEFFNLKSERRNGMMIYMIIIVMSFFVFLFVIGVLSSTFLTTMAEAGEA
                     VAKSGSSQMFMGGVDLFLYNRLFSHSALIQGFFSGLAAGVMGEGRVLAGLKYSAIMVL
                     IAWIAFRFFI"
     gene            13864..14187
                     /locus_tag="PLV90_04710"
     CDS             13864..14187
                     /locus_tag="PLV90_04710"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOF96448.1"
                     /translation="MKVRAIITGRGVHDVGYRLYLLERALDTGFQRFYAKIQVQNGAE
                     QVVVQYEGEPGQVDALGSIIREEHPPGVDIEHIAFEACAWDVVPVTDYLDLLIVEQLS
                     LIAPL"
     gene            complement(14312..>15549)
                     /locus_tag="PLV90_04715"
     CDS             complement(14312..>15549)
                     /locus_tag="PLV90_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HOF96449.1"
                     /translation="LLKPKGDRTSQVVLLGRPYKDVQIQDYYTETDLLLSEAVSAFER
                     LRERYGHVVVEGAGGAAEVNLYDRDIANIRLARALRLPIVLVADIERGGVFAQVYGTL
                     ALLPEDIRPLVAGVIVNKFRGDPWLFASGVAKLEELTGVPVLGVVPYTDIPLPSEDSL
                     SLADKGRQAAGGAIRIAVVRLPRIANFTDFELLERHAAVDYVPPGAPLSGYDCIILPG
                     TKNTVEDLAALSCHGTGEELRRARERGVPVIGICGGYQMLGSRIVDAGTESGTPGEHP
                     GFGLLDVTTTFSGYHKTTVQVRRRATGPGPILSRMGEVAGYEIHMGETERGSTLEAFA
                     GEGAVTPDGLVFGTYMHGLFQNPGAVNALLAYLSERRGVPFEPATAGGDAAYDALARH
                     FEEHVDMDAIVAWFGDGEE"
BASE COUNT         2815 a         4948 c         4758 g         3028 t
ORIGIN      
        1 acgggcctgg agggattcga acccccgaca tccgggttag aagcccggcg ctatgtcctg
       61 gctaagccac agaccccttc agtattccca tacattttag cggtcctgcg atattaaccc
      121 tttgaggccc cggggtgccc caaaaaaagg gagggcgtac tcacccggcc cggtaacacg
      181 ccgaccggat gatctcgacc atggtcgggt caccccactt gcgctcctcc acgccgcggg
      241 cgatggtcgt cccccggaac tggaccttga cccgggccga catccgttcc cgctcctcgt
      301 agtagagcgg gttgacgacc tcgacccgct tcatgctgat ggtgagggcg aggaggtcga
      361 ccatgatccc cgagtccccg ggcgagaggt cttccccgtt gaccgacgtc atgaagatac
      421 actcgcccgg ggcggcctcg gtcagcgtga tgatgttgtg ggcgctgttc agctcgaggg
      481 tgcgtgccct gccggtcttg agctcggcaa tgaccgacgg tgatatcccg gttaatgctg
      541 cgcacttcat cactcatcac ccgtacatcg gcagctgctc tcttcgtggt tgcgcctcgg
      601 tcttctgcac ctgctcggcg atctgggcca tggccgcctc gatctccacc gcctgctcga
      661 ggagcggctg aacgtccagg tcgaggtcgt atatcttgtt caagacctcg atcgtggccg
      721 cggaggaccg cgggtcgggg gtgttcaccg tctccccgag gagtccgatc cccggtatgc
      781 cccgggtttt gcactccgtg aggatgcttg ccgcgatgcc ggatatgctc cccatgggga
      841 ggatgatcgt ctgctcctcg atccgctgga ggaccgtgct ggtggttgca accccaaaaa
      901 ctctcttgtc gggctcgttt gtgatgatcc ccgcgatcgt ggcgatctcg cggatgccga
      961 actgggtgag ccagtccatg atgccgtttg ccacctcata gcagatcatg ggatggacgg
     1021 ggacgtcgga tatgatcgcg gcgaggctat ccttctcgta gaggcgtacg ggtgcgttga
     1081 tgacgccccg cgtcatgagg gcgaccggcg gaaagtattt gctcgtgatc gccccgatct
     1141 gctcgaaatc catctgctcg acgaggtatt gcagggcaat actcccgacg agcccgctgc
     1201 cggggaaccc gatcagcacc gtcttttcgg cgccggcaag cgccctggat atgaccttta
     1261 tatcgtccat gggatcaatc acccactccc ggattagttt aacatagctg gcagtccctt
     1321 aaaaatatta tgcaggaata ccagatcaaa cgtggataca cgaaagatct tgcgaaatcc
     1381 atggtgcagg gcctccgcga ccagttcggg ctcgaacccg aagtcgcggc cgacggccac
     1441 tatatcgtcg cctacggggc gctccatcgc cttgaggtct ggtccggtgc gggaggcaaa
     1501 acactggtcg tcgataccga ggcaaacagg gatgccgggg acgaggcgat catcgatacc
     1561 aaccgccggt tcaggaacta cctccagcag gtgaccgggt ataccgcaaa agagcgggcg
     1621 aagaaagcgc agcaagcctt gaaaggaagc gaataaccgg gttctccggc cgggcgcctc
     1681 tttctggctc cgggaccgga tttttatcta ttcttcgccc tgccgccggg ccgccgtccc
     1741 cgcccgcctc ttcttcggtc tcggtgcggg aagtgcggca caggtgatcg ctgtcagttc
     1801 tctcaggaac tcccggtcat ccaggtcgtc gatcaggaag cagggttttg cccccggata
     1861 cggcgatgcg gtcctgccgt ccggcatgaa cttctttccc gcctccgtta tcttgacgag
     1921 aaactggttg tcgcaggcac aggcaaagaa cttcccgtcg cagtagatac cgtactcccc
     1981 gaacatcttc cggtacgtga tattgcctgc accggcgagc tgcccggcca cgtactccac
     2041 gaactccgaa ctcgacgcca tcattatcgc ctctactgca gagaccacgc cctgcactgc
     2101 tcgattctcc tgccgccgtc gccctgccgc tcatcgaacg cgaactgcac gagaacctcg
     2161 caggggaact cgccgcaccg gccgcagttc tccagccctc tcgcttcgca gcaggtcttc
     2221 agcgggcaga tttcgcccca gaacggtctt tttatcgcca tacatccccc acaacccatc
     2281 gatgcccggt agccgcaccc gctgcagagt ataccgcacc gcgactcgat caccctcata
     2341 cgctcctgcc ggtactccgc tgccgcaaac aggccggata accgtctgtc ctgttcccgg
     2401 gttccgacga actctccgtc gaagaggagc atcggcatac tcaggtctcg tgatggggag
     2461 atatacctga ccccgcccgg ggtgagcatg cgcgggatat cgcgggacaa ccgtgcggat
     2521 gagatccctc cggttatgcc gtaaaaaaga ggaggtttct actcgatcat gatcgccggc
     2581 ttgaagggca tggccgccgc cgccttcggg tggttgctct ccccggcatc tttgaccgtc
     2641 accggcacgc cgaactcgtg ctccaggaat gcccgtgcgc cctcgaggac cgcccgctca
     2701 tcgaggggcg acgccgcaag ctgtttcacg atatcgggcg gcagcttcag gacgagtttc
     2761 gtgatctgct ttgccgcgtc cgtcgcctcc cggccgcgcc tccgcatact ctcgttctgc
     2821 atgatctccc gcatcaccct cgtcttgtcc gccgaaccgg ctatgatccg gaacgcctcg
     2881 tacttccagg ccggggcgac gaagagactg atggacgcgg gctccatcgg tatcagtttg
     2941 acgatcgact cgatatcctc gaccgtccgc gcgaggagct cctcggcgag ctccactccg
     3001 gggttgatcc ggctctcgtc cgtctccggc cagggggcaa aggagaccat gccgctctcg
     3061 ccgagatcgc tccagagcgc ttcgcaggtg aaggggatga agggggcaag gaggcggacc
     3121 cacgtgcggc agaggtcctg cagcaccgcc ccgccgcccg caccctcggg caggcggcgg
     3181 cggtaccact tcaggtcgga ctccacgccg aagaacgcct cctggagcgc ctgccgggtc
     3241 tggaacgagt caagagccgc ggtggcggcc tcgatacggc gctcgagccg gcttgcaagc
     3301 caggcgtcga tgggcgtggc cccctccgcc tgcatcgcct cggagaccgt gttccagaac
     3361 cgctcgatct gtctcttcgt cgacgagacg agctcgttcc tccagtcgaa gtcctgccag
     3421 ggctcggcgc tcccgacgag gaacatcctg acggtatcgg cgccgaactc ctcgagggca
     3481 tcctcgagca ggaagacgtt ccccttgctt gaggacatct tcgccccgtt caggaggccc
     3541 atcccgaaga ccaccatgcc tttcggttgc aggtacgagg ggaatatggc ccggtggtgg
     3601 aagagctgga acgtgaggtg gttggatatg aggtcctttg ccgagaaccg gtagtcgtag
     3661 ggataccagt agaggaactc gctcctgatc gcatcgaggg tctcccggtc gacggtctcc
     3721 ggagaaccca tcccaaagaa gatatagtca aataccgcgg gcgtcagctt ctccggcggt
     3781 atggctttta agtggtgggc gatcgtgtag taggccatgt agatcgtcga gtccgagagc
     3841 ggttcgatga tccaggtcgg gtcccaggga agcttcgtgc cgagtcccac ccgccgggtg
     3901 caggcccagt ccttcagcca gtcgaccgtc cggatgaact ccgccctgac ctcggggggg
     3961 acgagcgcca tctcctcgag ctgggtcttg acctgttcct tccagcacgg gtcgctgtac
     4021 tccaggaacc actggtcgtg gaggatcttt acaaagaccc tgccgccgca ccggcagatc
     4081 accaggcggt tgtcgaagtc gtacatcggg accgagtcgt agcgctccat catcagggcc
     4141 gccacgtcgt cgcgggcctc ccgcaccggt ttgccgccgt acttctcaaa gaccttcccg
     4201 cgggagaact cagcgctgta gacctcctgg gtgagcgcct ccatcccggg gtcgttctgg
     4261 tcgtggatgc ccgcacgctc gaccgcatcc tttgccggta tatcgccgta accctcgacg
     4321 gcaatgagcg gtatgggctc tacggacgtg tactgcccct gctgctgcag gtcgcggaga
     4381 gcgatgtaat caaacggcgc atgggcggga acgctcatga cgatcccggt ccccatatcc
     4441 ggatccacga acgtcgccgg gaggacgggg acgtcgccgg agagcgggtg ggtaaccgtc
     4501 tggtcgacga gcgcggtccc ggggatcttc tccgtcacgg tgacggtgtg atcctgcagg
     4561 gagagttttg ccgccgcctc agagcttacg atccactcct tgccgtcgat catcgcccgc
     4621 acgtaggtga cggccggatt cacccagagg ttcgtcacgc cgtagatcgt ctcgggccgg
     4681 agggtggcgc acgggacccg ggcgttgccc caccggaaca ccaccagggt gaaccggatg
     4741 atctcggcct tgtcgccctc gaggaggtcg tggtcgccga cagggttctc gcactgggga
     4801 cagtacttga ccgggtgggc gcccctgacg acgtgctcgt cctcccgcag gtgcttatac
     4861 tgccactcga tgaacttgct gtacgggggg tcgacggtga tgaaccggcg ccgccagtcg
     4921 atcgagagcc ccgacttctg catcacccgc cggtactcct ccgagaagta gcggacgatc
     4981 tccatcgggt tgatgaaccg gtcgaggatg tcctgcggca cccggtagag gtcgcggtag
     5041 agcccgacgg tcttttcatc gccgtttgca atccgcttcg agatcccgat gaccggcgtg
     5101 ccggtgacgt ggaacgccat cgggaagaga acctgcttcc ccgccatccg ctggtagcgg
     5161 gcgaggatgt ccgggacgat gtaggtcctc ccgtgcccga cgtgcatcgc cccgctcggg
     5221 tacgggtatg ctacggtcag gtagtacttc tccttctctc tctccgggtc ggcctcgaac
     5281 gcatgctccc accgggcgat acactcccgc tcgttgctct gcatatccag tccgctcacg
     5341 ctatgcctcc aaaatctgga tcactcgtca tattgttccc ctcataccgg ccgcagccgg
     5401 atcgtctccc cttcgttgta gcgtttgatc atctcctgtg cgatcgtgct gttcttcgca
     5461 aggaggatat ccccctcctc gttcaccgtt gccgtgaaga ggtactcttt cccggcaaag
     5521 acatcgacga tcttgccctt gttctccggt gagatgatgg tcagctgttt cttgtcgatg
     5581 cgtatcctga tgccgccggc aagctgcatc tcctcttcga cgggtttctt cgcctcaagc
     5641 tcgctgtgcg gccggatatc gatcccgatg ccgagtttgt tcacgatcgc cgagacgttc
     5701 ttgccgccct tcccgatcgc cgccgggaca tccttatcct cgatgtagac gacggctttg
     5761 ttgtcgctga tgatccggac atcgaccggg ccgctcgtgt agcgcccgat ctctcgctgg
     5821 acctctttct cggcaacctt ccaggcgacg ttctccccgg gttcgggtgc tgccgcggcg
     5881 gcggcgacga ccggcgccgg ttcgagaacg gcgggcggtt caacggcgac ccgggtgagc
     5941 ggcatcacca gggtctcgcc ctcgtaacgg aagatctcga tctcggtctc ctgccgggcg
     6001 tggtcgcgga cgacgatcac cgggcggacg tgcgtctcgc cgggcatgcc ctccggcgcc
     6061 ttgatcgtca gttcaaggtc gtacatcttc gtgatcgcac cctggacgac gaatatgacg
     6121 gtgttgatga tctggggcag gatgctgtag tccacgcggt cgcagaaccg ctgaaggcag
     6181 tcgtgcacct ccatcgcgtg caccacgccg accatgccca taccggcaag gcgcatgtcg
     6241 gcgtagacgt tgaagtcctc gttcttccgg atctcatcga atatcacgta gtcgggccgg
     6301 acgagcagga gggcttcggc ggtgtttgcc atgctcccct cgagcgccat gtactgggtg
     6361 atgtgatcgg gcacctgcag gtcccgcggc gcctccatcg tcttcacgat gaagtcgtgg
     6421 tcggcaagga acgtcgcgag gccctgggca agcgtggtct tccccgcacc cggggggccg
     6481 gcaatcagga cgccgcggcg gttcccgagg atcctctgcc ggatctccgg cgccatatcg
     6541 tagtcgtcga gcgagaggtc gacaagcggc cgcaccaccg tgatctccat cccgtccgca
     6601 aacggccgcc gggcgatcga tatccggagc gatcctatct ggacgaccgt gatcccccgc
     6661 ttctcgagtt cgatgaaccc gtccgggtcg cgttttgccc tctcaaggag ttcctgcgcc
     6721 attgaccgga gctcgtactc gctcatcggc gtatcgcgga gggtgacgag ccgtgtgtcg
     6781 cgaagccccc ctacctttgc aaccggcagg gcccgctctt tcaaggtgat cgcgatcgtc
     6841 tcctcatcga agaactggtc gatcgagagc ggcgagaaat cgcctatctg cggccgcagg
     6901 tagacgacgt caagcccttt tgcctttgcc acctcggcct gcacgaggtc gctcgtgatg
     6961 aaccgggcgc cgtatacgat agcgacattc cgtataaggg cgtcgatctc gccgccgctt
     7021 gagagtttca cctggtcgag gctcgggcgc tctccggaga actggagctc gatgatgccg
     7081 tccaccgaca tcctgaagag ctcctggagt tcggtcaggc cggagaaccc tatctcccgc
     7141 ccctggttcg cctgtgcttc cagttcggcg atgacggctt ccggtatgat tattgttgcg
     7201 cctttatatt ctcccgtttt tatcagagat gttatgcgtc catctatcac gacgcttgta
     7261 tcgggtacaa ttttcataaa aaatcaccat agtaatgagg tcattcaccc ttattagcgt
     7321 ataccttctt cggatcgaag accattccgg cgatctcttc gccatccgcc gtccggtaga
     7381 agcacgaggc atggccggta tggcaggcgg caccgacctg ttccacctca tagaggactg
     7441 cgtctgcgtc gcagtcgacg agtatgcggc agactcgctg gacatgtccg ctctcctctc
     7501 ctttcttcca gagtttctgc cggctccggc tatagtaatg tgcgtatccg gtggtccggg
     7561 tgagttccag tgccagagcg tttgcgtagg cgagcatcag gacatcgcgg gtccgtctct
     7621 cctgcacgat gacaggcacc aatccgttat cgttgaattg tatctccata ttgattcaca
     7681 ctcctgcaaa agtcgccatg atgacgaaga ggatatctcc tatgaataac gccgtcagga
     7741 atcccgccgc gatcggtatg atgaacggga tgccgtagga tatccagacc ctgccggcct
     7801 tccggtagag agcgagttct ctctcgtaat cttccgggtg cagcctgagg tcttttgtgt
     7861 agatgcgctg ctccccccgc accatgcgcc cgagcgaccc ggcaaacccg acaaaccgcc
     7921 tgaccagcct gccgtcctct tcctcgatat cctccatgac aaagccgaac tcgttctgga
     7981 tcgtcttccc gtcgacggga aagccgagga agaggcaggg gaacggggca cggttcccct
     8041 cgacgaggtt ctttgcaagt attgcaacgg gtgcggcgat gttgatgagc acggcgttcg
     8101 tcaggacggt gaatggaaaa aacccaaggg gcgggatgcc gaagtacggc tcgaccggga
     8161 agaacggtat gcacgcggtg atgatgatga gagcatacgc gtccgccccc ccaaagaggt
     8221 ttcgtgccgc aaagaggtag aagaaaccgc agaagaccac gaccaggacg aggtagccgg
     8281 ctgccgcctg ccagtctgcg agggtggcaa gcccgtatac gatggctgct gccgggacgg
     8341 cgatggcgag tgcggggcgc caggtcctgt ggggcacccg ccgctctctg gcgtcgagga
     8401 tcgacgcgta tatgagcgtg actccgatcg cggccgctgc aatcatgagc gggatggatg
     8461 cgaggatcat ggacaggtac gatatccacc ttgcatatct tatttctggc gattctttcc
     8521 gccgggcgga ccctcccggg acggcggcaa gaccgccggc ggtctctccg gggccgggat
     8581 caccgctctt ctggccgcga ttcaggagcg ttcgcagagt tctcgcccgt atatggccgc
     8641 atcagggttt tctgatcgaa tactttcatc gagtcgtccg ccggaaccgg tatcggacca
     8701 tcacaaggta ttaataatag catcgtaatg aggtatattg tggcaggggg taaaatcccc
     8761 tctccgacat aaaaaccact gaatatcaaa cctgaagatc tgcatggaca taaacggcct
     8821 gatgaacgag atccttctgc aatcgacgta cgaaggatgt tttactcttg acgagttgct
     8881 gcaccacggt agcgttcact ccctgaccgg aataggggct tcaaaagagc agacgcaggc
     8941 gttcttcctg gttctccttg ggggcgaacc ggacggcgct atttttatcg acgagaaggg
     9001 aacgctcttt ggtgatacgg cggtcctgca cctgaccggg caggaggagt tcaacctcca
     9061 ccgggtggcg ccgccgatcg ccgatgcact ggtggcacgg tgcagggtct ttgagaagag
     9121 ccacttgaag aagagcggcc gcctcgagat cccgacgatc ggtgcccctc cccgccagcg
     9181 ggtgggggtg ctctgcctta ccgtacagga caaccgggta ccccttgccg gggcccatgt
     9241 ctctatccgg aaggggaaac ttgtcatcac gggcgatgtg acggactccg aagggcgggt
     9301 ctgtttccgg ctgctcgccg ggcgctatat gtgcgtggtc acggatcgga ccggcgagcg
     9361 gacacggtgc atcatcgagt tccatgagcc tcaggttgaa acgtttgttg atattggggg
     9421 cacagaggat gagagcacat gaggatgaag aacttggatt gatagcagca gtcaggaatc
     9481 ttcttacaca atccgggggc ccgggttccg gcaacggaga taccctggcg gagccgcagg
     9541 gcactgttcc ggcaccgtgg cccgatgagg tggagatccg gggcgaaacg gacccggatc
     9601 ccggcgagac cgatgatacg gcggcgtttc cccctgtagc ggcgccagag agcgaggata
     9661 cccgcattct cgcccgaccc gggcataccg gggatcggcg tgcggccgac agggaggatc
     9721 cgatcagggc gctccttgac cggatagagc ggcgcaggat gggagaagag gctgcatccc
     9781 cgccatcgcc ggtggcggaa gagaccggaa ccacgccggc acccttcggt atcctcggcc
     9841 gcatgcgggg gcgaaaggat gccggggcgc ctcccctcga cgagcccgac ccggtcgatg
     9901 atgccggcgc acctcccctc gacgaatccg acccggtcga tgatgccggc gtgatctcgg
     9961 tcgaggagct cgggaacctg gcggacctga tcctcccgaa gagcgcgacg ttcacggtcg
    10021 atgagctgag catcaaccac cacgagcacc ggtttgactt cgtcgataac gcccgggtcg
    10081 tatcggagtt cgacgacctc tttgccaggg cgttctccac caccacgctc accgcggcag
    10141 agatcgcggt cccggcagaa ccggaggtac agtcccggtt cggattcctc aaaaagttct
    10201 acccgactgc cggggagatc atcgaggaat acaacctcac gctccacggg ccgctcgtcg
    10261 atctctctct gcggccgaca ccgggagtcg aggagatcga gctctatccg gtgaacgagc
    10321 cgtatgccta cgtccgggtg acctacgaca gcacgaccca cgagtacacc taccacgtcc
    10381 ttgagcccgt gctcacgccc ggggagcaag aactcctctc ggagatcaaa gaacgcctct
    10441 ttgagacgct caacatcgct acccgcgatc tcacccgcga tgcggcgaaa aaggcgctcc
    10501 ggggtgcggc aaacacgatc gtcgccgatt acgggatccg ccttcccccg ccggggcggg
    10561 agaagatcct ctatcacgtg gagaaggagt tcctcggcga cggcctgatc gacccggtga
    10621 tgcacgacaa gtatatcgag gatatatcct gcgacggcgt ggggagcccg atattcgtct
    10681 accacacgac ctacgaatcg atgaggacga gcctcgttta ccggaacgcc ggggaactcg
    10741 actcgttcgt cacgaaactc gcgcagaggg ccggaaagta catatcgatc gccgaaccga
    10801 tgctcgatgc cacgatgacc gacgggtcgc gcatccagat gacgctcggc tcggaggtga
    10861 ccgctcacgg ttcgacgttc acgatccgca agttccgcga tgaaccgatc acgcccacag
    10921 atctcatcga gtggcacacc ttctcgccgc tcgggatagc cttcctctgg cttgcggtcg
    10981 agaacgccaa atcctgcata tttgccggcg ggacggcttc agggaagacg acgaccttaa
    11041 acgccatatc gctcttcatc ccgccgcttg caaagatcgt gacgcttgag gatacccgcg
    11101 agctgaaact tcctcacccg aactggatcc cgagcatcac ccgcgactcg ttctcgcagg
    11161 acgggcgggg cgagatcgat atgtacgagc tcctgcgtgc cgccctccgg cagcgccccg
    11221 agtacatcct ggtcggcgag gtccgcggcc gcgaggccct gaccctcttc caggcgatga
    11281 gcaccggaca cgtcacctac gcgacgatgc acgcggattc ggtcgcaagc gccgttcacc
    11341 gcctggaaaa cccgccgatc gacgtgccgc ggaacatgct ctcggctctc tccctggtgt
    11401 ccatccaggt gcaggcccgg gtgggcggcc agcggatccg gaggaacaag cagctcatcg
    11461 agatactgga tatcgacccc cggacgaacg aactgatcac gaacgaggtc ttcaggtggc
    11521 acccggcgac cgacgagatc cgctactccg ggaagtccta catactcgag cagatcatgg
    11581 aggaccgggg ctggagcgag gaacggatgc gcgaggagct gaaacggcgc caggaggtgc
    11641 ttgaatggat gcgtatcaag aagatccgcc acttccgcga cgtgagcaag atcctggttt
    11701 cctacttccg tgacccggag tcggtcatcg agcgtgtgcg caccgacctc tacggggagg
    11761 ggggcccggc atgaacgggt atgaacggtt ctgtttcaac ctgctcggcc ggaacctgaa
    11821 ggcgaagcgc ggggagtacg tcagcctcag aaacgacctg acgggagccc ggatgaatac
    11881 gccgtttgag gcctacctcg cgaccgccta cgtctcctcg atcgccgtgg ggctggtcgc
    11941 cgccgcgctc atcgggattc tgacctacgc tctccgggtt cccgagatga tatcgtatca
    12001 cggcgcggtc ccggaggtct tctacgcgtt gaacgcctac aaactcctga tcggcaccgt
    12061 cgtcatcacg ctcctctccc tcctgatatt cggagggata acctacctga tcttcctcct
    12121 ctatcccggc atacgggccg gagagcgccg gagaaacatc gacgccaccc tgccctacgc
    12181 catcaactac gtcaccgcga tgtcttcggc ggggatcacc cccgacgagg tcttccggct
    12241 gctcggccag agcaggatat acggcgagag cgccgtcgag gcgcgttacg tcgcccgaga
    12301 gacggatttc ttcggcaagg atcttcttga cgccctccgg tcggtctcgc aggcgacgcc
    12361 gagcgagcgg atgcgggagt tcctgcaggg ggcggtcgca agcatatcga gcgggagcaa
    12421 cctcacggag tacttccgcg ccaaggcccg acagtatacg cttgagaaca accagcagca
    12481 gaagacgttc cttgagacgc tcggcctgat cgcggagtcc tacgtcaccg cgatggttgc
    12541 cggcatgctc tttttgatca tcctgcagtc ggtgatgacg atcctctcgg gcgatgcaaa
    12601 cccgttcttc ctctacatca tcatctacct gatcgtgccg ttcgggagca tcatgttcgt
    12661 catcctgatc agttccatga ccccggaggt gtgatatggc attcaaagag atcatcgatg
    12721 atttcctgaa caggaaccaa ccccggggtc ccgcggcgga ggaatctttt gcggacgaac
    12781 cgtttgggga acgggagata ttttcgcgga tcgagaaccg gcggaagtac caggaagggt
    12841 tctaccggtt catccggcac ccgctccggg tgatgatcga ggaccccgtc accgtcctct
    12901 tcgtatccgt cccggttgcc ctcttcctcc tcatcggcgg gtccgcgagc ctggtgatgt
    12961 cgtatggtcc cggcatcctc ctcacgacga cgatggtcga cgacatattc gtctttgctc
    13021 tcctcatcgc catcgtcccg cttgcggccc tcgacctgaa ggaggcgctc cgggtatcga
    13081 gcatcgaggc ctcgctcccg aacttcttcc gcgacgtggc cggtatgaac gactcgggca
    13141 tgacgctccc gcacgccatc cacatcgtct cggagggcga gtacggggcg ctgacgccgc
    13201 atatccgcaa gctcgatacc gagatgtcct ggggcgtccc gttcgtggag gccatccgcc
    13261 ggttcgggaa ggtcgtgaac accccgcttg ccgagcggag cgtggacctg atcgcccacg
    13321 cgagttctgc gggcggcgac gtgagcgagg tgctgcgggc tgcggcgcac gacgcctacg
    13381 agttcttcaa cctgaagtcg gagcggagga acggcatgat gatctacatg atcatcatcg
    13441 tcatgtcgtt cttcgtcttc ctcttcgtca tcggcgtgct atcgagcacc ttcctcacca
    13501 cgatggccga ggcgggggag gcggtcgcaa aatcgggctc gagccagatg ttcatgggcg
    13561 gcgtggacct cttcctctac aaccggctct tctcccactc cgccctgatc caggggttct
    13621 tctcggggct cgcggcgggc gtcatgggcg agggccgggt ccttgcgggg ctgaagtact
    13681 ccgcgatcat ggtgctcatc gcctggatag cgttccggtt cttcatctga agagccgggg
    13741 cagagtctct ttttttcgaa ggccggttcg tggcgtgatg atccgtacgg gccggagcgc
    13801 cggatcgcag gtgttcgtag ccgctccgga gggtatatga cctttccgga gagatgccct
    13861 tctatgaagg tgcgggcgat catcaccggc aggggagtgc atgatgtagg ttaccggctc
    13921 tacctgctgg aaagagccct cgataccgga ttccagaggt tttacgcaaa gatccaggtg
    13981 cagaacggcg ccgaacaggt ggtggtgcag tacgagggcg agcccgggca ggtggatgcg
    14041 ctcggctcta tcatccgtga agagcaccct cccggcgtgg atatcgagca tatcgcgttc
    14101 gaggcctgcg cgtgggacgt cgtccccgta accgattacc tggatctgct catagtcgag
    14161 cagctctccc tgatcgcccc cctctgaggt ctctctccgt tgcatcttcg cgtggggctg
    14221 tatcgttacc cacaccagtc gcctcccgcg ttggcgatag aggatgccag agcctcgtgc
    14281 aggagtgggc gatgatgcga gcgccggccc cctactcctc cccgtcccca aaccaggcca
    14341 cgatcgcatc catatccacg tgctcctcga agtgccgggc gagggcgtcg taggcggcgt
    14401 cgccgcccgc cgtcgccggc tcgaacggaa ctccccgccg ctccgaaaga taggcgagga
    14461 gggcattgac ggcgccaggg ttctgaaaga ggccgtgcat gtaggtcccg aagaccaggc
    14521 cgtcgggggt tacggcccct tccccggcaa acgcctcgag cgtactcccc cgctccgtct
    14581 cgcccatatg gatctcgtag ccggccacct ctcccatccg cgagaggatg gggccggggc
    14641 cggtcgcccg tcgccggacc tgcaccgtcg tcttgtggta gccggagaag gtggtggtga
    14701 catcgaggag accgaacccc gggtgctcgc ccggggtccc cgactcggtc ccggcgtcga
    14761 cgatccggct cccgagcatc tggtagcccc cgcatatacc gatgaccggg actccccgtt
    14821 cgcgggcacg acggagttct tcccccgtgc cgtggcaact cagggcggcg aggtcctcga
    14881 cggtgttctt cgtccccggg aggatgatgc agtcgtaacc ggagagcggc gccccgggcg
    14941 ggacgtagtc caccgccgcg tggcgttcga ggagctcgaa gtcggtgaag ttcgctatcc
    15001 ggggaagacg gacgacggcg atccgtatcg cccctccggc cgcctgccgg cccttatcgg
    15061 cgagagagag cgagtcctcg ctcgggaggg ggatatccgt gtacggcacc acgccgagca
    15121 ccgggacacc cgtgagctcc tcgagtttcg cgacccccga tgcaaagagc caggggtctc
    15181 ctctgaactt gttgacgatg accccggcga cgagcggccg gatgtcctcc gggaggaggg
    15241 cgagcgtccc gtagacctgc gcaaagacac cgccccgctc gatgtcggcg acgaggacga
    15301 tcgggaggcg gagggcgcgg gcaagcctta tgtttgcgat gtcgcggtcg tagaggttca
    15361 cctccgccgc tcccccggcc ccctcgacca ccacgtgccc atagcgctcc cgcagccgtt
    15421 caaacgccga gacggcctcc gaaaggagca gatcggtctc cgtgtagtag tcctgtatct
    15481 gcacatcctt atacggccgg ccgagcagca ccacctgcga cgtccggtcc cccttcggtt
    15541 tgaggagga
//
LOCUS       DAOSII010000041        14864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_4162_length_14864_cov_41.599973, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000041 DAOSII010000000
VERSION     DAOSII010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 14864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14864
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4162_length_14864_cov_41.599973"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1487
                     /locus_tag="PLV90_04720"
     CDS             <1..1487
                     /locus_tag="PLV90_04720"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HOF96450.1"
                     /translation="MKESTRFAFDISWVFVSQIVTLGSGFLLNIVLSRWLGPADFGLY
                     TLTLTIFTISTLIAGLGIPETVVKYVADYRSDQERLNAVVTAGVINALVLGVAAGLAL
                     FFFAGDIAGSFGMPELSGLIRIIAVAVPLAIVNTTLLGLLNGLREMRSYSFRFMIRSL
                     LLITLAVLFIAAGLGPGGAVLALLLTEVGTLAFVVSIVRRHFRLSVPGYPETSRKMLA
                     FGSRVFIASAVYLINTYTDTLLIGYMLTEIDVGRYAIAIAIARVAFLSLPGSISTVNY
                     PAISEYYSKGLHDAVEAVITRSIRYCLILLSTMGLALIYLAETIIGFLFGPAYLPAVP
                     AFIILVFGMIVFGAVSSVGPAVSAMNRPGLSSKVNIFVAGTNVCLDVALIPLFGITGA
                     AIGTAGACTLLAVLILYVLHHVFGVRVESAMIIKAFGVVSVFFIAFLLLRGTVHPYVL
                     TVVFSGLYGIYAYRFLLTVEDKTEIAGMIAPVFGRIAARRLNGR"
     gene            complement(1504..2487)
                     /locus_tag="PLV90_04725"
     CDS             complement(1504..2487)
                     /locus_tag="PLV90_04725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF96451.1"
                     /translation="MVFDYTDSVSPGVWRSFLNGGEGIYFFHTPEWAKVMEGAFGYRV
                     ATRLYHTPEGDVLVPMMESRRFGFSVYQSVPHGYGGIFSPSSLSAGVLTDLVGSMVGG
                     RHLLLDLALPPGVALQRERSPGVTKTLSEWNYTHILRLDDPGNAVEQRYNRHARRDIG
                     IAERNHVEIVRDDSLAQFQRYYALYEQRAREWGYPEPPYPRKVYDLLYRYGRPYVRVR
                     LAEMQGEVIGGLVTFEYADTVFAWSNAAPAASRPFCPVHLLLHDALRSAQEEGYSLVN
                     FGGSGNLLGVRKFKESFGAVQVDLETYRITSRLASVMRKGWWPAYLRKSSG"
     gene            complement(2477..3940)
                     /locus_tag="PLV90_04730"
     CDS             complement(2477..3940)
                     /locus_tag="PLV90_04730"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOF96452.1"
                     /translation="MLARSRAYLNVEQIVDDLSREDLEDESHIRTFEDRFCSYIGAER
                     CHATNQARAALLIALRALDLSPGDEVLVPDFTFEGVVDAILESGATPVFVEPALQDFN
                     VPASHFIEKMTERTRAVIATHLFGIPCDIGDLPAIAKENDCVVIEDCAQCLGAEYNGR
                     PVGTFGDFTIVSFNYEKHLTTGEGGMLAVNNPTFAGDVERVVGSCTRVPLHDEKCYVY
                     GMLVQHTATERAVYRRDLLAYFGQNCCRDNPDIFGLVDDMVLQGEPWEDVRTALLPHV
                     EREIERAGYVPPHMRNAVVREVLKRAGEARSRVFCPPIRTIGTGHLLMGALRALVGTL
                     GLEELPAVNAARNRNARLFYGAMQDTPGFPLPEISPGKVPAFLKCNILNRTHHPLSLI
                     VGRARDRGYEVGNIQWPRPVHLLPRFRHAAGRQRDVFPLTGYLTGRIINIPIHCGVTS
                     EDIEGMAACLREFSSGAGSGVADRLRVTGVEAATHGV"
     gene            complement(4080..4715)
                     /locus_tag="PLV90_04735"
     CDS             complement(4080..4715)
                     /locus_tag="PLV90_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOF96453.1"
                     /translation="MIADLHIHTRYSHDSFMDPARVVRVARMRGLSAIAVTDHNTIRG
                     GLLAREANPGGDFEVIVGAEIRTEYGDVLGLFLDREIAARRFDDVVEEIHAQGGLAVL
                     AHPYRRYPSPERLAGRVDLIEGFNSRSRRQANALSMRFGRASGKGTVGGSDAHTYAEI
                     GRGVTIYAGSEIEKALRAGQTAGEGRESNYYVVHGLSCGIERLKRLVPGRG"
     gene            complement(4712..5881)
                     /locus_tag="PLV90_04740"
     CDS             complement(4712..5881)
                     /locus_tag="PLV90_04740"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOF96454.1"
                     /translation="MAWKIPLSDIAMDGEEVDAVRDVLQSRWLSMGPVTEEFEAAFAR
                     YLGAEHAFAVSNGTAALHIAHAVLGIGSGDEVITPSLTFVATANSVLYCGARPVFADI
                     AGPDDLNVAWEEIAAKITPETRAITVVHYGGYPCDMQPILDLARDHDLRVVEDAAHAV
                     GATYRGQKCGTIGDVGCFSFFANKNLATGEGGMVVTDDDDLAERIRVMRSHGMTTLTW
                     DRHRGHGYSYDVTAIGYNYRTSEVASALGIVQLRRLAENNARRKRIAGRYRKLLEGVE
                     GIECPFAGAVDKESSHHIFPVVLSQDLSRAGLQEALKEKGIQTSIHYPPIHLFTYYRG
                     LFGDTRGSLPKTEFAGEHEMTLPLYPGMTDDEVEYVCTAIAGAAPGILAGGRGAA"
     gene            complement(5883..6599)
                     /locus_tag="PLV90_04745"
     CDS             complement(5883..6599)
                     /locus_tag="PLV90_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOF96455.1"
                     /translation="MKAVILAGGKGRRLEPYTTIIPKPLMPIGDKPILEIILCQLREH
                     GIRDVTIAVGHLAELIMAFFGDGSRFGLSITYSREERPLGTAGCLGLIKDDLTEPFLM
                     MNGDVLTTLPFSELMDFHQKSGAIATVALNKRDYFVDFGIVDTNGNGDIVGYTEKPGF
                     EHYVSMGVYAFDPRVLAYITPHEYIDFPDLIKALLAAGETVKAYKYGGYWLDIGRVDD
                     YKKANNEIAGLYGTLGVGGE"
     gene            complement(6601..7590)
                     /locus_tag="PLV90_04750"
     CDS             complement(6601..7590)
                     /locus_tag="PLV90_04750"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOF96456.1"
                     /translation="MDWTEKRVLVTGAGGFIGSHLTESLVDHGADVRAFVRYNSRNDP
                     GLLRLLPPERLDRVRILMGDLRDADAVRRAVRDVDVVFHLGSLIAIPYSYARPRETVE
                     TNVIGALNVFEAARDAGVEKVVHTSTSEVYGTARYVPIDENHPLQGQSPYSASKIGAD
                     KVAESFYRSFDVPIATIRPFNTYGPRQSARAVIPTIITQALVQGKVALGATHTTRDYT
                     YVDDLVRAFLLIAESPGTVGETINVGSNYEISIGAVAGTVAALLGKEIDIITESERLR
                     PEKSEVERLWCDNTRARDLLGWSPAVPLEEGLLRTIAWFREHLGLYRPNHYVT"
     gene            complement(7770..8792)
                     /locus_tag="PLV90_04755"
     CDS             complement(7770..8792)
                     /locus_tag="PLV90_04755"
                     /inference="COORDINATES: protein motif:HMM:NF023385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOF96457.1"
                     /translation="MAVNALSIDLEFWWCNEFLNGYLPGDREDMFKESLRPIFASLDA
                     FNVRATFFVLGAVAERYPEVIHDIHRRGHEIACHAYSHKPLYSMDQREFERELGKSLE
                     LLADYNPVGFRAPSFSLSNRTKWALESLEKYGFQYDSSIFPVRTPLYGAPDAPVGIYR
                     PSRDDITVHDPDGPIIEFPLSIVRFGVNIPISGGFYLRSLPKQVLAWGIGRVAAKRPA
                     NIYLHPHDIYPGPPPLKVPFLSRLVTYHGVHTSLEKLIMLMMKFSFMPIGALLDEMSL
                     LSPEAPVPMAPARPLRGVGGRDDPAVPTIPAPTLQLQPFDGVRQRYPARAARPIHETG
                     DPDETL"
     gene            complement(8794..9966)
                     /locus_tag="PLV90_04760"
     CDS             complement(8794..9966)
                     /locus_tag="PLV90_04760"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOF96458.1"
                     /translation="MSRGAVFVTDAQMRSSLAVIRSLGKQGLDVTAGEETRCATGVFS
                     RYCRRGLVYPSPKKAEERFIGYLLDTLRSGEIDVLFPVADACLKPIVDHEEEISRYTR
                     IALPPRDIFTAGYDKGTTLCIAMENGIPCPRTFFPESPDDAAGIAEKIDYPAVVKPRV
                     SSGRRGVRVCRDSQDLVQAYAASASEFGPVVIQEFIPYGGELGVYTLLNAQSEPRAVT
                     VQRRIRSYPISGGASTLRETIKDERSREAVGIALRLLKAMRWSGVAMVEFRVDARDGS
                     LKLMEVNPRFWGSLNLSILSGVDFPYLLYRMVMDGDIEPVLDYQEGVLCRWLLPGDIL
                     WYLSAPKTWENLKEFLRFDTPDDILSLHDPGPTLGFALATARYALDPDMWRFVLRR"
     gene            10531..11697
                     /locus_tag="PLV90_04765"
     CDS             10531..11697
                     /locus_tag="PLV90_04765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOF96459.1"
                     /translation="MVVTPYFYPHMGGVQKYVYEIGTRLQRVFGHDLTIVSSNWHKEE
                     NTAKTEEIGGLEVVRLPYQFKVSSTPVSIGWERALRDLIRSERPDVVNGHMPVPFIAD
                     AAARAAAGMQVPYVLTYHNDLTGHDLPTRIIGRCYYPLLGSRTLHLSDAIVATSDYYA
                     QQSSHLAGYLEKLRYAAPGVDLERFGGDRTDFLRRKYRLGDEKILLYVGHLDRESRHK
                     GVQYLIRAMRIVRESLDAKLVVAGRGNYIDRYRRLAADEGVPEDIIFAGFIPEDEMPL
                     YFNSADLLVLPSCDRAEGFGMVLIEAQACGTPVIGTDVGGIPFAVDDGVTGLIVPPGD
                     VSGLAGAIVRVLDDPDLYRAMAENGPRRVRDLFTWEKSARVYDRVFCEAAGERA"
     gene            11921..13924
                     /locus_tag="PLV90_04770"
     CDS             11921..13924
                     /locus_tag="PLV90_04770"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOF96460.1"
                     /translation="MDTAFLEQILIIFALSIAALALCYRMKMPGIVAFFLTGIIAGPY
                     GLGLITNRADVEILAELGIIFLLFTIGMELSLKSLLDMKKGMLTGGVLQIGLTIGIVA
                     VIASALGLAPPEAIFFGMLLAHTSTTVMLTVFQHRGEVDTPPVRLALGISVLQDLSTV
                     PMILLVPMLGGMGSAGVVPSLINFALGLVLLAAVTASALWVVPRFLYRVASLRSRELF
                     MITVITLCLGTAWLTSRAGLSLALGAFLAGLIISESEYSNAALSTVIPFRDVFTSFFF
                     VSMGMLLNTGFFAAHALLIVAIIVGVIVLKAIIGAIAVLPAGVSLRTAVLTGLAIGQI
                     GEFAFILSRTGIEHGLLSPDLEQIFLAVSVGTMAVAPFVIASAPQATAVVRRLPLPVW
                     LRARDASLKVAPANGPGEGHIVIVGFGPIGRHVAEAARSTGVPYMAIELNPKTVREER
                     KKGEPIFFGDAINEGVLVHAGVERARVVVITIPDAATARQVTGIARKLNPDCAIITRT
                     RYMENSLALYAIGANDVVCEEFETAAKVLSRVLVRYPVQKSDIDRLVASMRTGNYEML
                     RRASGQVPLLHDIGLHLDNAEVSIVRVDEGAPIAGKTLAEADLRRRYAVTVLAVRRGT
                     ETLAGPDGGTVIEAGDLCILVEAGPGNPDISSLFRHGTEGQGS"
     gene            14053..14640
                     /locus_tag="PLV90_04775"
     CDS             14053..14640
                     /locus_tag="PLV90_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HOF96461.1"
                     /translation="MDFLSAGALTPILIPLLVFGARVIDVSLETLRIILLARGGRLIV
                     PAIAFVEIIFWIVSLGLVVNEFTNPIYLISYGAGFAAGNYAGILLEERLAMGICVLRV
                     IASEEDHALIDAIRGTGYGVTVVAAEGLHGPCTIFYSVVRRCDLFPITRMVEEMNPGA
                     FCTVEDVRSTTHGTFPRAGERTLSGIPRRTVRLGK"
BASE COUNT         2681 a         4767 c         4599 g         2817 t
ORIGIN      
        1 ccatgaaaga atctaccagg ttcgcattcg acatcagctg ggtatttgtc agccagatcg
       61 tcaccctcgg ctcggggttt ctcttaaaca tcgtgttgag ccgctggctc ggcccggcgg
      121 acttcgggct gtacaccctg acgctgacca tctttaccat atccacgctg atcgcaggcc
      181 tcggcatacc cgagacggtc gtgaagtacg tggccgacta caggagcgat caggagcggc
      241 tgaatgccgt cgtcaccgca ggagtcatca acgcgctcgt gctcggggtc gctgccggtc
      301 ttgctctctt cttctttgcg ggagacattg caggatcttt cgggatgccg gaactctccg
      361 gcctcatccg gatcatcgcc gttgccgtgc cgcttgcaat cgtcaacaca acgctccttg
      421 gcctcctcaa cggcctgcgg gagatgagat cctactcgtt ccggttcatg atccgctcgc
      481 tcctcctgat caccctggcg gtgctgttca tagccgcggg cctcggaccc ggaggagccg
      541 ttctggcgct gctgctcacc gaggtcggga cactcgcctt cgtcgtctcg atcgtccgcc
      601 ggcacttccg cctctcggtc cccggctacc ctgagacgtc gaggaagatg ctcgcgttcg
      661 ggagccgggt cttcatcgca agcgcggtct acctgatcaa cacctacacc gatacgctcc
      721 tcatcggata catgctgacg gagatcgacg tcggccggta tgcgatcgcg atcgcgatag
      781 cccgggtggc cttcctctcg ctccccggtt cgatctccac ggtcaattac cctgcgatat
      841 cggagtatta cagcaaagga ctccacgatg cggtcgaagc cgtgataacc aggtcgatcc
      901 ggtattgcct gatcctcctc tcgacgatgg gcctcgccct catctatctc gccgagacga
      961 tcatcgggtt cctcttcggc cccgcctacc tgccggccgt gcctgcgttc atcatactcg
     1021 tcttcggcat gatcgtcttc ggggcggtgt cttccgtcgg gccggccgtc agcgccatga
     1081 acagacccgg tctctcctcg aaggtgaaca tcttcgtggc ggggacgaac gtctgcctcg
     1141 acgtggcgct catccccctc ttcggcataa ccggtgcggc catcggcacg gccggagcgt
     1201 gtacgctgct cgcggtgctg atactctacg tgctccacca cgtctttggt gtacgggtcg
     1261 agtccgcgat gatcataaaa gcgttcgggg tcgtcagcgt atttttcatc gccttcctcc
     1321 tgctgcgggg tacagttcac ccctacgtgc tcacggtggt cttctctggc ctgtacggca
     1381 tctatgccta ccggttcctg ctgacagtgg aggataaaac ggagatcgcc ggcatgatcg
     1441 ccccggtatt cgggcgcatc gcggcacggc gtctcaatgg gcggtgagca ggcacgcccc
     1501 gggctacccg gaggatttgc gaagatacgc cggccaccag cccttccgca tgaccgacgc
     1561 cagacgggac gtgatccggt aggtctcgag atcgacctgc acggccccga aactctcttt
     1621 aaacttccga accccgagaa ggtttccact tcccccgaag tttacaagcg agtaaccctc
     1681 ctcctgtgca gaccgcaggg catcgtggag aaggagatgc accggacaga aagggcggga
     1741 cgccgccggg gccgcattgc tccatgcaaa gaccgtatcc gcatactcga acgtcacgag
     1801 acccccgatc acctcgcctt gcatctccgc caggcgcacc ctgacatacg ggcggccgta
     1861 gcggtagagg aggtcgtaga ccttccgggg atagggcggt tccgggtagc cccactcccg
     1921 cgctctttgc tcatagaggg cgtagtagcg ctgaaactgc gcgaggctat catctctgac
     1981 gatctcgacg tggtttcgct cggcaatgcc gatatcccgg cgcgcatgcc tgttatacct
     2041 ctgctcaacc gcgtttcccg gatcgtccag gcgcaggata tgagtatagt tccactcgga
     2101 gagcgtcttc gtgacgccgg gggatctctc tctctgcaat gcgacacccg gcggcagggc
     2161 gagatcgagg aggaggtgcc tccccccgac catgctcccg acaagatccg taagaacccc
     2221 ggccgagagg ctcgaaggcg agaagatgcc gccgtagccg tgagggacgg actggtagac
     2281 ggagaacccg aaccgccgtg actccatcat cgggaccagg acatcgcctt caggggtatg
     2341 gtagagcctt gtcgccaccc ggtagccgaa cgccccttcc atgaccttcg cccactcggg
     2401 cgtatggaag aagtagatcc cctccccgcc gttgagaaac gatctccaga cgcccggaga
     2461 gacggaatcg gtgtaatcaa acaccatgcg tcgctgcctc aactccggta accctcaggc
     2521 gatccgccac gccggacccg gcgccgctgc tgaactcgcg aaggcaggca gccatgccct
     2581 cgatatcctc cgatgtcacg ccgcagtgga tgggaatgtt gatgatccgt ccggtcaggt
     2641 acccggtcag ggggaacaca tccctctgcc gccccgcggc gtgccggaac cggggcagca
     2701 ggtgcaccgg cctcggccac tggatgttcc ccacctcgta cccccggtcc cgcgccctcc
     2761 cgacgatcag ggagagcggg tgatgggtcc ggttcaggat gttgcacttc aggaacgcgg
     2821 gcaccttgcc gggtgagatc tccggcaacg ggaaacccgg ggtatcctgc atggcgccgt
     2881 aaaagaggcg ggcgttccgg ttccgggctg catttactgc cgggagctcc tcgagcccca
     2941 gggtgcccac gagagcccgc aaagccccca tcaacaggtg gccggtcccg atcgtcctga
     3001 taggcgggca aaagaccctc gacctggcct ccccggcgcg cttgagcacc tccctcacga
     3061 ccgcgtttct catgtggggc ggcacgtaac cggcccgctc gatctcgcgc tcgacgtgcg
     3121 ggaggagggc ggttcggaca tcttcccacg gttcgccctg gagcaccatg tcgtccacga
     3181 ggccgaagat atccgggttg tcccggcagc agttctgacc gaagtaggca aggagatccc
     3241 ttcggtagac tgcccgttcc gtggctgtat gctgcacgag catgccgtag acgtagcact
     3301 tctcgtcgtg gagcggcacc cgcgtgcagg atccgacgac ccgctccacg tctccggcga
     3361 aggtggggtt gttgacggcc agcatcccgc cttcgccggt cgtgaggtgt ttttcgtagt
     3421 tgaagctgac gatcgtaaaa tccccgaacg tcccgacggg cctcccgtta tactccgcgc
     3481 cgaggcactg ggcgcagtcc tcgatgacaa cgcaatcgtt ctctttcgcg atcgccggca
     3541 ggtccccgat atcgcagggg atcccgaaga gatgcgtcgc aatgaccgcc cgtgtccgct
     3601 ccgtcatctt ctcgataaag tgcgatgccg ggacgttgaa gtcctgaagc gccggttcga
     3661 cgaagaccgg ggtcgctccg gactccagga tggcatcgac gacgccctcg aaggtgaagt
     3721 ccgggacaag cacctcgtcg cccggcgaga ggtcgagggc ccgcaacgct atcaacaggg
     3781 ctgcacgggc ctggtttgtc gcatggcacc gttctgcccc gatgtaactg cagaaccggt
     3841 cctcaaaggt tctgatatga ctctcatcct caagatcctc acgtgacaga tcatcgacga
     3901 tctgttcgac gtttaagtat gctcgagacc gtgctaacat tccttccccc gagatagtgg
     3961 atacggagtg gtgatagtaa accaaatata taaatataat ccagtgagaa acccaggcaa
     4021 gaccaatgcc ggccggtatt ggtcagaggg gccacgaaca gtactcccgc cgggagcact
     4081 caaccccggc cgggaaccag acgttttaac ctctcgatac cgcagctcaa cccgtgtacg
     4141 acatagtagt tcgactcccg gccttccccg gcagtctgcc cggcacggag cgccttttcg
     4201 atctcacttc cggcatagat cgtcacaccc ctcccgatct ccgcgtaggt atgcgcatcg
     4261 ctcccgccga ccgtcccctt cccggaggct cgcccgaacc gcatcgagag cgcgttcgcc
     4321 tgccgcctcg accggctgtt gaacccttcg atgaggtcca cccttcccgc gaggcgctcg
     4381 ggcgatgggt accggcggta ggggtgtgcg aggaccgcaa ggccgccctg ggcatggatc
     4441 tcctcgacca cgtcgtcaaa ccggcgcgca gcgatctccc ggtcaagaaa gagacccagg
     4501 acgtcgccgt actccgtcct gatctcggcg ccgacgatga cctcaaagtc cccaccaggg
     4561 ttcgcctccc gggcaaggag accgccgcgg atggtgttgt ggtccgtcac ggctatggcg
     4621 gagaggcccc gcatccgggc tactcttacg accctggccg gatccatgaa cgagtcgtga
     4681 gagtaacggg tatggatgtg cagatcggcg atcatgccgc cccccggcct cccgccagga
     4741 tgcccggtgc ggccccggcg atggcggtgc agacgtactc cacctcatca tcggtcatgc
     4801 cggggtagag cggcagcgtc atctcgtgct ccccggcaaa ctccgtcttc gggagagagc
     4861 cccgggtatc cccgaacagc ccgcggtagt aggtgaagag gtggatgggg gggtagtgga
     4921 tgctcgtctg gatccctttc tccttcaacg cctcctgaag cccggcccgc gagagatcct
     4981 gtgagagaac gaccgggaag atgtgatggg aggactcttt gtcgaccgcc ccggcaaacg
     5041 ggcactctat gccttcaacc ccctcgagca gcttccggta gcggccggcg atgcgtttcc
     5101 gtcgcgcatt gttctccgcg aggcgccgga gctggacgat gccgagcgcg gaggcgacct
     5161 cgctcgtccg atagttgtag ccgatagcgg tgacatcgta actgtagccg tgcccccggt
     5221 gccggtccca ggtcagcgtg gtcatgccgt gcgagcgcat gacccggatc cgttcggcaa
     5281 ggtcgtcgtc atccgtcacg accatgcctc cttcgccggt ggcgaggttc ttgtttgcaa
     5341 agaagctgaa gcacccgacg tccccgatgg tgccgcactt ctgcccccgg taggtcgcac
     5401 cgaccgcatg cgccgcatcc tcgacgaccc ggaggtcgtg atcgcgggca agatccagga
     5461 tcggttgcat atcgcaggga taccccccgt agtggacgac ggtgatcgcc cgcgtctccg
     5521 gcgtgatctt tgccgcaatc tcctcccatg ccacgttcag gtcatccggg ccggcaatat
     5581 cggcaaagac cggtcttgcg ccgcagtaga ggaccgagtt tgccgtcgcg acgaacgtga
     5641 gcgacggggt tatgacctcg tcgccgctgc cgatgccgag caccgcatgg gcgatgtgca
     5701 gagccgccgt cccgttcgag actgcaaacg catgttcggc accgaggtac cgggcaaagg
     5761 ccgcctcgaa ctcctccgtc accggcccca tcgagagcca cctcgactgc aggacatccc
     5821 tgaccgcatc gacctcttcg ccgtccatgg cgatgtctga gagagggatc ttccacgcca
     5881 tatcactcgc ccccgacgcc cagcgtccca tagagcccgg cgatctcgtt gttcgccttc
     5941 ttgtagtcat cgaccctccc gatgtccagc cagtagccgc cgtatttgta ggctttaact
     6001 gtctcgcccg ccgcaagcag ggccttgatc aggtcgggga agtcgatgta ctcgtggggc
     6061 gtgatatacg cgagaactct cgggtcgaac gcgtagacgc ccatgctcac gtagtgctca
     6121 aaccccggtt tctcggtgta cccgacgata tctccgttcc cgttggtatc cacgatacca
     6181 aaatcgacga agtaatccct cttgttgagg gcaaccgtcg cgatggcgcc gcttttttgg
     6241 tgaaagtcca tgagctccga gaacgggagg gttgtcagga cgtcgccgtt catcatgagg
     6301 aacggttcgg tgaggtcgtc cttgatgagc ccgaggcagc ctgcggtccc gagcggcctc
     6361 tcctcccgcg agtaggtgat cgagagacca aacctgctcc cgtcgccgaa gaacgccatg
     6421 atcagttcgg caaggtgccc gaccgcaatc gtcacgtccc ggataccgtg ctcacggagc
     6481 tggcaaagga tgatctcaag gatcggttta tccccgatcg gcataagcgg cttggggatg
     6541 atggtcgtgt agggttcaag ccttcgtccc ttgccgccgg caagaataac cgctttcata
     6601 tcaggtcacg tagtggttgg gccgatagag gcccaggtgt tcccgaaacc atgcaatcgt
     6661 acgcaaaagc ccttcttcca ggggcacggc gggcgaccag ccgaggagat cccgcgccct
     6721 ggtgttgtcg caccagagcc gttcgacctc gctcttctcc ggccgcaggc gctcgctctc
     6781 ggtgatgata tcgatctctt ttccgaggag ggctgcaacc gtaccggcga cggcccctat
     6841 cgatatctcg tagttcgacc cgacgttgat cgtctccccg accgttcccg gcgattcggc
     6901 gatgaggaga aacgcacgca ccaggtcgtc gacgtaggtg tagtcccgtg tcgtgtgcgt
     6961 cgcaccgagc gccaccttcc cctgcaccag cgcctgcgtg atgatcgtcg gtatcaccgc
     7021 ccgcgccgac tgccgcgggc cgtaggtgtt gaacggccgt atcgtggcga tggggacgtc
     7081 aaaagaccgg tagaaactct cggcgacctt gtcggccccg atcttgctcg ccgagtaggg
     7141 ggattgcccc tggagcgggt ggttctcgtc gatggggacg tagcgggcgg tcccgtagac
     7201 ctcgctcgtc gatgtgtgga cgaccttctc gacgccggca tcccgggccg cctcaaagac
     7261 gttgagcgca ccgatgacgt tcgtctccac cgtctcccgg ggacgggcgt aggagtaggg
     7321 gatggcgatg agcgaaccca ggtggaagac gacgtcgacg tcccggacgg cacgccggac
     7381 ggcgtcggca tccctcagat cgcccatgag gatacgcacc cggtccagtc tctccggtgg
     7441 gagcagccgc aggagaccgg ggtcgtttct tgagttgtag cggacaaacg cccgcacatc
     7501 cgcaccgtgg tctaccaggc tctcggtcag gtggctgccg atgaaaccgc cggcgccggt
     7561 cacaagcact ctcttttcag tccaatccat cacatacgac tcctttaacc ctgaacgcgc
     7621 gtacggctgc gagcagactc tggatacacg atccagtata taatactatc tcaaatcccg
     7681 ggatcgcgcc tgcccgggac atgccggttc ccgcagatct ccccatcggc ccggtgccgg
     7741 ggatgccgcc ccggtgcccg tggtgagcgt tacagcgtct catcgggatc acctgtttcg
     7801 tggatcggtc ttgcggcacg ggccggatac cgctgtctga cgccatcgaa gggctgcagc
     7861 tgcagcgtgg gtgcgggaat cgtcgggacg gccggatcgt ctcttcctcc caccccccgg
     7921 agggggcggg caggcgccat cgggacaggg gcctccggag aaaggagcga catttcgtca
     7981 aggagcgctc cgataggcat gaacgagaac ttcatcatga gcatgatcag tttctcaagc
     8041 gatgtgtgga cgccgtggta ggtgacgagc cgggagagga acggcacctt caggggggga
     8101 ggacccgggt agatgtcgtg ggggtgaagg tagatgtttg ccggcctctt tgcggccact
     8161 ctgccgatac cccaggcaag aacctgcttt ggcagggaac gaagatagaa cccccctgat
     8221 atagggatgt tgacaccgaa ccggacgatc gagagcggaa attcgatgat cgggccgtcg
     8281 gggtcgtgga cggtgatatc gtcccgggag gggcggtaga tcccgaccgg cgcatccggg
     8341 gcgccgtaga gcggtgttct caccgggaag atgctcgagt cgtactgaaa accgtacttc
     8401 tcgagcgact caagtgccca cttcgtcctg ttgctcaggg agaacgacgg cgcccggaac
     8461 ccgaccgggt tgtagtcggc aagcagttcg agggactttc cgagctccct ctcgaactct
     8521 ctctgatcca tcgagtagag cggtttatgg gagtaggcgt ggcacgcgat ctcgtgacct
     8581 ctccggtgga tatcgtggat gacctcagga tacctctccg cgacggcacc aaggacaaag
     8641 aacgtcgctc tgacgttgaa tgcatcaagc gatgcgaaga tcggcctcag cgactccttg
     8701 aacatatcct cgcggtcgcc gggcaggtag ccgttcagga actcgttgca ccaccagaac
     8761 tcaaggtcga tgctgagtgc gttgacggcc acgttatcgc ctcaggacaa accgccacat
     8821 atccgggtcg agcgcgtagc gtgccgtggc gagcgcaaac cccagcgtcg ggccggggtc
     8881 gtgcagcgac aggatatcgt cgggcgtatc gaaccggaga aactctttga ggttctccca
     8941 ggtctttggg gcgctcaggt accagaggat atctccgggg agcagccagc ggcacaaaac
     9001 cccctcctgg taatccaaga ccggttcgat atccccatcc atgaccatcc ggtagaggag
     9061 gtagggaaag tccacgccgc tgaggataga cagattcaac gatccccaga agcgagggtt
     9121 cacctccatg agtttgaggc tcccatcccg ggcatcgacc cggaactcga ccattgcaac
     9181 cccggaccat cgcatcgcct tcaggagacg gagggcgatc ccgacggcct cccgggagcg
     9241 ttcgtccttg atggtctcgc ggagggtgct tgcccccccg gatatcggat aagagcggat
     9301 ccgacgctgg acggtcaccg cacggggttc tgattgtgcg ttgaggaggg tatagacccc
     9361 gagctctcct ccgtagggaa tgaactcctg gatcaccacg gggccgaact ccgatgcgga
     9421 tgccgcataa gcctggacca ggtcctgtga gtccctgcag accctgaccc cccgccttcc
     9481 tgaactcacg cgaggtttga cgacggccgg gtaatcgatc ttctctgcta tgccggccgc
     9541 atcgtccggg ctctccggga agaacgtgcg cgggcagggg atgccgtttt ccatggcgat
     9601 acagagggtc gtgcctttgt cgtagcccgc cgtgaagatg tcacggggcg gcagggcgat
     9661 ccgggtgtac cgcgagatct cctcttcatg gtcgacgata ggcttcaagc aggcatcggc
     9721 gaccgggaag agtacatcga tctccccgga cctcagggta tcgaggagat acccgatgaa
     9781 ccgctcctcc gccttcttcg gcgatgggta taccaggccc cgcctgcagt acctcgagaa
     9841 aactcctgtc gcacaccgcg tctcctcacc cgcagttaca tccaatccct gtttaccaag
     9901 cgaccggatg accgcaagag agctccgcat ctgcgcgtcg gttacaaaga cggctccccg
     9961 actcaacact tcccccccgg gcctgtgagc tacgttgtag cgagcggtgg atcgcctctt
    10021 taagtattta aacctattct ggaacttcgt ggtctcaatc cggcatcagg actatggggg
    10081 gtgacatacg ggtgagaagg gcgacatcgc ttcatcaccg gatagcctgg ccatcgggat
    10141 acgatatgca gcataatgcc ggaaactgag gggatctcaa aggcgacagg ttcggtacat
    10201 caccgggtct ccggtacggg agggggaagc agcgatcatg gtgccgggga gggagagatg
    10261 ttcatcgagt gcgtcgcctc cgggggcggc atccccggcc ctccggactt ctcccgggtg
    10321 aaccgtgggg atatcgccgt tgggagcggg gcgccccggg ggtttccttc cggcacctcc
    10381 cccgcgaaga tcgggggtgg gatgggacag cgtatacgcg gccgtgatga gaggtcgggt
    10441 gaacctcaga gccccgcggg cttgaagata gatttatatg agtgattgtg caccctcacc
    10501 ccatccggag ggtgagagag cgtgaaggcc atggtggtga ccccgtattt ctacccgcac
    10561 atgggtggcg tgcagaaata tgtctacgag atcggtacga gactgcagcg ggtattcggg
    10621 cacgacctga ccatcgtctc ctccaactgg cataaggagg agaacactgc aaagaccgag
    10681 gagatcggcg gcctcgaggt ggtccggctg ccctaccagt tcaaggtctc cagcaccccc
    10741 gtcagcatcg ggtgggagag ggcgctccgg gacctcatcc ggtcggagcg cccggacgtc
    10801 gtaaacgggc atatgccggt tcccttcatc gcggatgcag ccgcccgggc agcggccggg
    10861 atgcaggttc cttacgttct gacctaccac aacgacctga cggggcacga ccttccgacc
    10921 cggatcatcg ggcggtgcta ctacccgctc ctcggttccc ggacgctcca cctctccgat
    10981 gcgatcgtcg ccacatcgga ttactatgcg cagcaatctt cccacctcgc cggatacctc
    11041 gaaaagctgc ggtacgccgc cccgggcgtc gaccttgaac ggttcggggg agaccgaacg
    11101 gacttcctgc gccggaagta tcgcctgggg gacgagaaga tcttgctcta cgtgggccac
    11161 ctggacaggg agagccggca taaaggcgtc cagtacctga tccgggccat gagaatcgtc
    11221 agggaatccc tggatgcgaa actggtcgtc gccggcaggg gaaactacat cgaccggtac
    11281 aggaggcttg ctgccgacga gggagtcccc gaagatatca tattcgccgg tttcatcccc
    11341 gaggacgaga tgcccctgta cttcaacagc gccgacctcc ttgtcctgcc gagttgcgac
    11401 cgggcggagg ggttcgggat ggtgctgata gaggcgcagg cgtgcggcac gccggtcatc
    11461 gggacagatg tcggcgggat accgttcgcc gtcgatgacg gcgtgaccgg cctcatcgtc
    11521 ccgccagggg atgtgtcggg gctcgccggc gccatcgtgc gggtgctcga cgatccggac
    11581 ctttaccggg cgatggcaga gaacgggccg aggagggtgc gggacctctt cacctgggag
    11641 aagagcgctc gcgtctacga tcgggtcttt tgcgaggcgg ccggggagcg ggcgtagacg
    11701 cgagcgagag ggagcagccc gccggcgagc cccggcaacc ggatctgccg tgacaatccc
    11761 ggggaaaatc cgggttcgtg catactgtgc gaggttacac ctcatcgggt tttatcagaa
    11821 gggaagagca acaactttct ctatatcacc ggcgggtgca ctcgctggag agcgccagca
    11881 ccatggcggc ccgccgggga cgacagccag ggaccatacc atggatacgg cctttctcga
    11941 acagatcctg atcatattcg ccctctccat cgcagcgctc gccctctgct accggatgaa
    12001 gatgcccggg atcgtggcgt tcttcctcac cgggatcatc gccgggcctt acggcctcgg
    12061 tctcatcacc aaccgggccg acgtcgagat cctcgcggaa ctcggcatca tcttcctcct
    12121 cttcaccata gggatggaac tctcgctcaa gagcctcctt gacatgaaga aggggatgct
    12181 caccggcggg gtgctccaga taggcctgac aatcggcatc gtcgcggtca tcgcctcggc
    12241 cctcggcctt gcgccgccgg aggcgatctt ctttgggatg ctgcttgccc acacgagcac
    12301 caccgtgatg ctgaccgtct tccagcaccg gggagaggtg gataccccgc cggtgcggct
    12361 cgcgctcggt atatcggtcc tgcaggacct gagcaccgtg ccgatgatcc tcctcgtccc
    12421 catgctcggc ggcatgggat cggccggggt cgtcccgtcg ctcatcaact tcgccctcgg
    12481 cctcgtcctc cttgccgccg tcacggcaag cgccctctgg gtcgttcccc gcttcctcta
    12541 ccgggtcgcg agcctccgga gccgggagct cttcatgatc accgtcatca ccctctgcct
    12601 cgggacggca tggctcacct cacgggccgg cctctccctt gccctcgggg ccttcctcgc
    12661 cggcctgatc atatccgagt ccgagtacag caatgcggcc ctctccaccg tcatcccctt
    12721 ccgcgacgtc ttcaccagtt tcttcttcgt ctcgatgggg atgctcctca acaccgggtt
    12781 cttcgcggcc cacgcgctcc tgatcgtcgc gatcatcgtc ggcgtcatcg ttctcaaggc
    12841 aatcatcggg gctattgccg tcctccctgc gggcgtctcc ctccggacgg ccgtcctcac
    12901 cgggcttgcc atcggccaga tcggcgagtt cgcgttcatc ctctcaagga ccggtatcga
    12961 gcacgggctt ctcagccccg atctcgagca gatcttcctt gcggtatcgg tcgggacgat
    13021 ggcggtcgcc ccgttcgtca tcgcgtcggc accacaggcg accgccgtcg tccgccgtct
    13081 tcccctgccg gtatggctca gggcaagaga tgcgtccctg aaggtagccc ctgcaaacgg
    13141 accgggagag gggcatatcg tcatcgtcgg gttcgggccg atagggcggc acgtcgctga
    13201 ggcggcacgg agcaccggcg tcccctacat ggctatcgag ctcaacccga agacggtgcg
    13261 ggaggagcgc aagaagggcg aaccgatctt cttcggcgac gccataaacg agggcgttct
    13321 tgtccatgcc ggggttgagc gtgcccgcgt ggtggtgatc accataccgg atgcggccac
    13381 ggcgcgccag gtgacgggga ttgcgcggaa attgaaccct gactgtgcga tcatcacccg
    13441 gacccggtac atggagaatt ccctcgccct ctacgccata ggggcaaacg atgtcgtctg
    13501 cgaagagttc gagaccgcag cgaaagtgct gtcgcgggtc ctggtcaggt acccggtgca
    13561 gaagagcgat atcgacaggc tcgtcgcttc gatgcggacc gggaactacg agatgctccg
    13621 gcgggcctca gggcaggtgc cgttgctcca cgacataggg ctgcacctgg acaacgccga
    13681 ggtctcgatc gttcgggtgg atgagggcgc cccgatcgcc gggaagaccc ttgccgaggc
    13741 cgacctgcgg cggaggtatg cggtgacggt gctcgcggtc aggaggggca cggagacgct
    13801 cgccgggccg gacggcggga cggtgatcga ggccggggat ctctgcatcc tcgtcgaggc
    13861 cgggcccgga aacccggata tatcctccct cttccgccac gggacggagg ggcaggggtc
    13921 gtaaccggag gtgcgcgccc acggcccggg aaggggcaga ctccggcgcc cggtcccgta
    13981 atccgcgcag atcccgggcc ttcgccggct cttgactgca ccctcatata cccggccggg
    14041 cgactctccc atatggactt tctctctgcc ggcgccctca ccccgatcct catcccgctc
    14101 ctggtctttg gggcccgggt gatcgacgtc agcctcgaga ctctcaggat catactcctt
    14161 gcccggggcg gccgcctcat cgtccccgca atcgctttcg tcgagatcat cttctggatc
    14221 gtctcgctcg gcctcgtcgt caacgagttc accaacccga tctacctcat ctcttatggg
    14281 gccggtttcg ccgccgggaa ctatgccggc atcctccttg aggagaggct cgccatgggg
    14341 atctgcgtgc tccgggtgat tgcctctgaa gaggatcacg ccctcatcga cgccatcagg
    14401 ggcaccggct acggggtcac ggtcgttgcc gcggaggggc ttcatggacc atgcaccatc
    14461 ttctattccg tggtgcggcg ctgcgatctt ttcccgatca cccggatggt cgaggagatg
    14521 aaccctggtg cgttctgcac cgtcgaggac gtcaggtcga ccacgcacgg gaccttcccc
    14581 cgggccggcg agaggaccct gtcgggcatc ccccgccgga cggtgcgtct cgggaagtga
    14641 acgggagatc ctgtcggcga gagcgtatgg gtaactacct gcgggaaggc gcgggaacga
    14701 atatcaagca gaacccggta gtccgggatc gatgcacggt cggcactcac gggggtgcag
    14761 aagagcggag catatcgcag agaaccggag atttgcccgg aaaaaaagat gataggagag
    14821 gttgaaggtt agttcttctt agccttgtcc tggggcttgt cctg
//
LOCUS       DAOSII010000042        14456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_4284_length_14456_cov_38.068398, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000042 DAOSII010000000
VERSION     DAOSII010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 14456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14456
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4284_length_14456_cov_38.068398"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..1507)
                     /locus_tag="PLV90_04780"
     CDS             complement(128..1507)
                     /locus_tag="PLV90_04780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF96462.1"
                     /translation="MTQHTKEECARQLQAIFQENDKDPAALRAAVQDFFRRLAADWSR
                     TIEDLRRENVLLQEARQCLCQDTHAEALERIQTYAEELATANEELETANEMLNNRNEE
                     LIAARKVAEAERERYYELFDAAPEGYLVTDRNGKVLEANRTAGVLLAVHKESLIGRNL
                     LSFARNENRVNLKAWMVQAVKGQGRQTFEADLLPCMEEPVHVSVSIVAVRGGEEGEPS
                     LRWMVQDISGRKRAEEALRRLNREVEAARDEANLYVDILTHDIRNTENVSNLYAELLI
                     DCLEGDEAQYAANLQRSIRKSIDIVGTISTIRQIHQTGSELRPVDLDAAIRDTVKGYP
                     RTIIHYVRTDRQVLADDLLPVIFNNLIGNAVKHGGPGVEIAIRAEEEDGFVRVSVEDD
                     GPGVPDEEKDAIFRRYEQQKRGVGAGLGLHLVQILIRRYGGRIWVEDRIPGTPGAGTA
                     FRLTLRKAA"
     gene            complement(1669..4587)
                     /locus_tag="PLV90_04785"
     CDS             complement(1669..4587)
                     /locus_tag="PLV90_04785"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOF96463.1"
                     /translation="MEQDEHGKEEAKRPLAIVGIGASAGGLEAFEQFFKNMPPDAGIA
                     FVLITHMDPAQKGLLPEILRRFTRMPVRETEDGMKVEANTVYVKPAQADLTLFHGTIG
                     HLEPVRTHGLRMPIDSFFRHLADDQDGKAVGIVLSGTGSDGTLGIRAIKERMGMVMVE
                     DPSSAQFSGMPRSAMATGLVDYSAPPEELPELLIQYVKFSATTPTGEKLTTARVEADL
                     RRIFSLIRLRNGQDFSRYKRNTIRRRIERRMALHQIPSIGEYVTFLYENPPEVDTLAR
                     ELLIGVTRFFRDQKAWVALKEGILKDLIPLRPEGGTIRAWSVGCSTGEEAYSLAIVLR
                     ECLDTLKRPDMVQVFATDVDARAIGIARRGTYPANITADVSPERLERFFVKEDNTYLV
                     HKEIRESVVFATQNVLADPPFTHLDIISCRNLLIYLSAELQKQLIPLFQYALNPGGFL
                     FLGTAESVGGFNTLFKTVDARWKIFRRQEGTTPQVPRFEIPAAVVAQPATAEKKAREF
                     AREDEIVTALAREWLLVQYAPPAVIVNEDGDILYFHGRTGKYFEPRPGRATLNIHTMA
                     RPGIRDALISLLQAATREREGFVQEAAPARVSDGEQQILITIRPVGQLAGRGGMLYVV
                     VFQNNEKPYRKYPEGGAEERKRSDRAPEGGTVQEIARVRFQIQHTIEDMQASQEELKS
                     MNEELQSTNEELQSMNEELTSSREELQSLNEELLTVNMEHQKKIAELSESNDDMRNLL
                     QITNIAMLFLDDDLRVRRFTTPIRPIVNLQAGDVERPITDLAVNLKEGGFSDDLRGVL
                     RTLQVREREVQTKEGRWFKMQIRPYRTAENRIEGVIVTFIDISQSRDLESSIRRARAC
                     AEEVMAAVHEPLLVLDDNHRVVSANRSFYTMFGVTREEAEGNLIYALQDRQWDIPRLH
                     ELLEEILPKQGEIEDFLVEHDFPGVGHRVMRFNARALPPDAGPDRILLAVGGSIDRPI
                     "
     gene            complement(4696..7662)
                     /locus_tag="PLV90_04790"
     CDS             complement(4696..7662)
                     /locus_tag="PLV90_04790"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HOF96464.1"
                     /translation="MAKGEVDPAETRPGEAVTPEEITEVPPFIITGIGASAGGLEALE
                     TFLKNTPPDIGAGFVVVTHMDPEHRSLLPEILQRSTTMPVVQIEHGMKVRQNSVYVIP
                     PNTNLTIQKGILNLEEPSRPRGMRLPIDHFFRGLAQDQDSKAVGILLSGMGHDGVLGL
                     RSLKERLGTVMVQDPITAEFPSMPRSVIVSGLADYIAPAGELPKLLANYIQSYRSLRA
                     ASPEEKRYLNTRTKILALIRSRTGEDYSIFKESLIRGRIERRMGLHRITDGEDYVRYV
                     QEHPREIDFLAQEIPTGPNWFFRDPGVWKILRERLLGELIRAKPGDSTLRVWVSGCAT
                     GEQAYSMAIALEETIQSSGSDIQYQIFATDIDRESILEARGGIYIANIEVDVSPEQLD
                     RFFTRQDNTYQIRQEIREKTIFAQHNILTHPPFLHLDILDVRSLLLYTSPDVVQKLIP
                     LFHYALNPGGILILGIDYSAGEPLEHFETIDATWKIYRRAAGPGGDDIQVELPSTLIP
                     SFGLVVGEPRLRKENEGSSIAALAREWLLSQYAPPAVIVNENGDILYFQGRTGKYLEP
                     HPGRATLNVHTMAREGLRYPLASTTRTALQEKRTVTRGPVFVQTNGSEEAIRLTVRPI
                     REIEGAEDLLMVVFEPVQDPAPEVGHEEGAAWKAATQPDLDKELAETRAQLQRTVEDM
                     QASQEELKSMNEELQSTNEELKSTNEELTSSKEELQSLNEELLTVNAEHQRKIEELSE
                     SNDDMRNLLQSTDIAMLFLDNDLRVRRFTNPIRPIVNLQAGDTGRPITDLHISILDED
                     IVGDVRTVLDTLQMQMKRVQTADGKWYLMRILPYRTTENRIEGVIITFTDTTPLKKMK
                     DSLEEARMYAESLVAIVHEPLLVLDADLRIISANRSFYTMFRTTRDETEGNLIYALGN
                     QQWNIPRLRSLLEEILPQQTKLDGFLVEHDFPVIGHRVMRLNARMLHSDTGPERILLA
                     IEDITGRDEQEREKTT"
     gene            complement(7871..8182)
                     /locus_tag="PLV90_04795"
     CDS             complement(7871..8182)
                     /locus_tag="PLV90_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOF96465.1"
                     /translation="MAAVTAEQVREAAGVLGIRDRASLNEIRRRYHDRIKEWHPDVSQ
                     NDPVKSHEMTLRLKEAYDLLVDYCMNQTLSFRTEDIAEGLGQNPTRHWMDRFGDDPIW
                     G"
     gene            8384..9838
                     /locus_tag="PLV90_04800"
     CDS             8384..9838
                     /locus_tag="PLV90_04800"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HOF96466.1"
                     /translation="MRPAPGEVIAMTGSSGAAIRLFDYGPIFLLRHCLRDLHLDRHLP
                     ELFPETEAAMVVALAFNRLIRPATMPYIDTWYAETALALDNPALSLSDREIGELLKKI
                     GTSDIPDTLADLIIEENETRRALVYDITSLSGSSSLEHGYSQDSLLLPPITLSLVVDE
                     DRGIPVMYDVYPGSITDSTTLAGTLARLEACGVTDCTAILESGFFSSDNLLDMLDEQI
                     SFVIAARFSMKELRALITREQRDIARAGYIREFHGRTIHVKPVVFALEGRALQGYLYY
                     DPGLEQEAREAFTRHLDDIREALFAVRLKPGQIADHVFSGIAGEFGGFFDCKQVEDRI
                     EATIRQSAVTLRTNRMGRYMLFYQGNADWMTCLSRYRERDCIEEEFERLKGELEALPL
                     EAHGTAAFHGYLFIVFLSLMLRSRLARMLEDAGLAETYSVEGVMLELEKWKKIVLADG
                     RVTTTRMTRKQKTILKALGLVPLPREAKEKGRPR"
     gene            10169..11365
                     /locus_tag="PLV90_04805"
     CDS             10169..11365
                     /locus_tag="PLV90_04805"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOF96467.1"
                     /translation="MTKVFLCTNGCVEGQLRTKFVERYLGAHAQNIHMASAIGDADLV
                     LFYACGLTGEKEGHSLSVIDELKEKMRQDAELVVWGCLPKQNPGALVGVYDGPIIGPM
                     DTGSITSLIGTNGVSPESIDRSAAASELATMASPSIHGCNGTDLLTAAFLLYKRGVDL
                     FGSRRQRRSEVYHIPIAAGCTGHCTYCSERPVFGNTVHSRPIDDIVSEFRGGLERGYS
                     RFSLFATDLGSYGIDIGCRLPDLLRDLVAIGPAENCSILLNQIEPHRFEEVYGDMEPI
                     LASGKIEMVMAPVQSGSNRILKLMGRHYTAERWSALMLDIYRNYPAIRLSTQFMVGFP
                     TETEDDFAATLSLLDPPLRLDEAHIFRFSPRPTVAASRLPGQVPDDVKEARAARLLSR
                     FARRRI"
     gene            11649..13121
                     /locus_tag="PLV90_04810"
     CDS             11649..13121
                     /locus_tag="PLV90_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOF96468.1"
                     /translation="MIRKMACTGFLVILVASVSMAVLLAFIQAGGIHTAGIPEKNAIL
                     VAARDSPESAKALADYICDGVADQVEIQQAIDASRSRGMIVSLAEGTFTLDGDLVVPD
                     DTTIEGKGDTRTHLHWISGRLTAYGGENIVLRDFETCGTGAIFFFNCSHVKVHDVTAR
                     IDNSLGGGAFFVWVQNSVSEDIEFVNCKAIDCGRMGFMNDGEGARRLIRDIRYINCQA
                     INSGRHERFAPYGEWTTGFAIAENNDLADAVLTGCLAEGSFESGFHVEDAPTVTNLVF
                     RNCISRNNGQKPDAFYNPSEESHGLHFGSGYWVQGETTLYNCVSEGNGNAGFSAGPGV
                     RLYSCTDDGSAVGFRLVETHDVYIEDCTSRNAGAYGVYALEAERIVAEGLEILDPAGF
                     DGSGSFFGTTAHPVVDSRFDIVGRNSETIRTIYGEGCRDVTFTGVIRTDHREPVVIAR
                     GAGVDTRGLQVEAKEEIASPGQDGGCGLKNFLIRIITAFL"
     gene            13118..14260
                     /locus_tag="PLV90_04815"
     CDS             13118..14260
                     /locus_tag="PLV90_04815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOF96469.1"
                     /translation="MKIVQVTAYYPPHTGGIERYVHNLSRALAGRGHDVTVLTANVPR
                     GRPFETENGVAIERRPCLVRPFNDPFVPSFFRPGESLAGVDVLHAHNAYSCAALSAGV
                     VRRKFGVPTVLTHHGQHMFGHPLKDLFVTLYRGTIEHGLVHRVDRVVTLSPSDAAYVS
                     AFGVGRERISVIPNAICPEDFLPCTSEDTAGFEEAHRLEGKKRVLFVGQISTRKGVDV
                     LLRAVDQIVHEQGRDDLVFLFVGDGDYRERAQELARRHRVEEYVRFAGKVGLCDLVAA
                     YRSSDVYVLPSLSEGMPTVILEAMFFGLPVITTDLPCLRDNFGDHAVLVPPRDEHALA
                     RAVTGLLDDAARCRRLSERGKELVRSNYTWDNVVLAYERLYEQMTD"
BASE COUNT         2411 a         4518 c         4612 g         2915 t
ORIGIN      
        1 cccgggcccg gcacttgatc aggtaaaaga gcatctccgg gttccggcgg tagtacacgc
       61 tacgcgcctt tcgccggaac tccgcggcag aggcgctctg ccggcagaac acccccgggc
      121 cgtgcgccta tgccgccttc ctgagcgtca gccggaacgc cgtgcccgca cccggggtgc
      181 ccggtatgcg atcctcgacc cagatcctgc cgccgtagcg gcggatgagg atctgcacga
      241 gatggaggcc gagccccgcc ccgacgcccc gtttctgctg ctcgtagcgc cggaagattg
      301 cgtccttctc ctcatccggc acgccggggc cgtcgtcctc gaccgagacc cggacaaacc
      361 cgtcctcttc ctccgcccgg atggcgatct cgacgccggg accgccgtgt ttcaccgcgt
      421 tgccgatgag gttgttgaag atcacgggga gcaggtcgtc ggcgaggacc tggcgatccg
      481 tccttacgta atggatgatg gtgcgcggat agcctttgac cgtatcccgg atggccgcat
      541 cgagatccac cggcctgagc tcggaacccg tctggtggat ctggcggatc gtggagatgg
      601 tgccgacgat atcgatgctc tttctgatgc tccgctgaag gttcgccgcg tattgggcct
      661 catctccctc caggcagtcg atgagcagtt cggcgtagag gttcgagacg ttctcggtgt
      721 tccgtatgtc gtgggtcagt atatccacgt agaggtttgc ctcgtcgcgt gccgcctcga
      781 cctcccggtt caggcgccgg agcgcctcct cggcccgctt gcggccgctg atatcctgga
      841 ccatccagcg gagcgacggc tctccctctt ctccccctct gaccgcgacg atgctcacgg
      901 agacatgcac gggctcctcc atgcacggca ggagatccgc ctcgaaggtc tgccgcccct
      961 gccccttcac cgcctggacc atccaggcct tgagatttac ccggttctca tttcgggcaa
     1021 agctgaggag gtttcgcccg atcaggctct ctttgtggac ggcgaggagc acccccgctg
     1081 tgcggttggc ctcgaggacc ttcccgttcc tgtccgtcac caggtagcct tcgggggcgg
     1141 catcgaagag ctcgtagtag cgctcccgtt ccgcctcggc aaccttccgg gcagcgatca
     1201 gctcctcgtt ccggttgttc aacatctcgt ttgcggtctc gagctcctcg tttgccgtag
     1261 caagttcctc agcataggtc tggatccgct ccagggcttc tgcgtgggtg tcctggcaga
     1321 ggcattgccg ggcctcctga agcagaacgt tctcccgtcg gagatcctcg atggtccggg
     1381 accagtccgc cgccagtctc ctgaagaagt cctgcacggc agcacggagt gcggcggggt
     1441 ctttatcgtt ctcctgaaat atcgcctgca gctgtcgtgc gcactcttcc ttcgtatgtt
     1501 gcgtcaaacc ggatctcatg atggtctgat ccctgctgga accccgcggc cggagaccgg
     1561 cagctgtatt ccaaccgtta caccaataaa ctcatatatc tgccgattgc ccttcgatcg
     1621 gtcccccgca ggaacaggcc ccgcttcagg tttgggcact tccggtacct atatcggccg
     1681 atctatcgaa ccgccgacgg caaggaggat ccggtcgggc ccggcatccg ggggaagcgc
     1741 ccgggcgttg aagcgcataa cccggtggcc gacgcccggg aagtcgtgct ccaccagaaa
     1801 atcctcgatc tcaccctgtt tgggcaggat ctcctcgagc agttcatgga gccgggggat
     1861 gtcccattgg cggtcctgga gcgcgtatat gaggtttccc tcggcctcct cccgcgtgac
     1921 cccgaacatc gtataaaacg agcggttcgc ggagacaaca cgatggttat cgtccagcac
     1981 caggaggggc tcgtgaacgg cggccatgac ctcttcagcg catgcgcgtg cccgcctgat
     2041 agacgactcg agatccctcg actggctgat atcgatgaag gtgacgatca cgccctcgat
     2101 acggttctcc gctgttcggt agggccggat ctgcatctta aaccatctcc cctccttcgt
     2161 ctgcacctct ctctcccgca cctgcagggt cctgagcacg ccgcggagat cgtctgagaa
     2221 ccccccctct ttgaggttga cggcaagatc ggtgatgggc ctctctacat cccccgcctg
     2281 aaggttgacg atgggccgga tgggggtggt gaaccgccgc acccgcagat cgtcgtcgag
     2341 gaagagcatg gcgatattgg ttatctgcag gaggttgcgc atgtcgtcgt tgctctccga
     2401 gagttccgcg atcttcttct ggtgttccat gttgacggtg agcaactcct cgttcaagga
     2461 ctggagttcc tcgcgggaac tcgtcagttc ctcgttcatg ctctggagct cttcgttcgt
     2521 gctctggagc tcctcgttca tcgacttgag ctcctcctgc gaggcctgca tatcctcgat
     2581 cgtgtgctgg atctggaacc ggaccctggc aatctcctgc accgtgcccc cttccggggc
     2641 acggtctgat cgtttgcgct cctccgcccc gccttcgggg tattttcggt atggtttctc
     2701 attgttctgg aagacgacga cgtagagcat gccgcctctt cccgccagct gcccgaccgg
     2761 ccgaatcgtg atcaggatct gctgctcgcc gtcgctcacc cgcgcgggag ctgcctcctg
     2821 gacgaacccc tctctctccc gggtggccgc ctggaggagg gatatcagcg catcccggat
     2881 accgggccgt gccatcgtgt ggatgttcag ggtagccctg ccaggccggg gttcaaagta
     2941 cttccccgtc ctgccgtgga agtacaggat atcgccgtcc tcgttcacga tcaccgccgg
     3001 gggggcatac tgcacgagca gccattcccg ggcaagggcg gtgacgatct catcctccct
     3061 tgcgaactct cgcgccttct tctccgctgt tgcgggttgc gcgactacgg ccgccggtat
     3121 ctcgaacctg ggcacctgcg gggtggtgcc ctcctggcgc cggaagatct tccacctggc
     3181 atcgaccgtc ttgaacaggg tattgaatcc gccgaccgac tcggccgtgc cgaggaagag
     3241 aaacccgccc gggttcaagg catactggaa gaggggtatc aactgcttct gcagttccgc
     3301 cgagaggtag atgaggaggt tacggcagga gattatgtcg aggtgcgtga acggggggtc
     3361 tgcgaggacg ttctgcgtcg caaagaccac gctctcgcgg atctccttat gcacgaggta
     3421 ggtgttgtcc tcctttacga agaaacgttc cagccgttcg ggggagacat cggcggtgat
     3481 gttcgccggg taggtgccgc gccgcgcgat cccgatagcc cttgcgtcca cgtcggtggc
     3541 aaagacctgg accatgtccg gccgcttcag ggtatccagg cactccctga gcacgatggc
     3601 gaggctgtaa gcttcctccc cggtggagca gcccacgctc caggcccgga tcgtccctcc
     3661 ctccggccgc aaggggatca ggtctttgag gatcccctct ttcagagcaa cccaggcttt
     3721 ctggtcacgg aagaaccgcg tcacaccgat aaggagttcg cgtgcaaggg tatcgacctc
     3781 cggggggttc tcatagagga acgtgacgta ctccccgatg ctcgggatct ggtgcagggc
     3841 catccggcgc tcgatacgcc ttcggatggt gtttcgcttg taccgggaga aatcctggcc
     3901 gtttcgcagg cggatgagag agaatatccg ccggaggtcc gcttccaccc gcgccgtggt
     3961 gagcttctcc ccggtgggag tcgtagcgga gaatttcacg tactggatga gcagctcggg
     4021 aagttcttcg ggcggagcgc tgtagtccac gagcccggtg gccatcgcgc ttctgggcat
     4081 gccgctgaac tgggccgaag acgggtcctc gaccatcacc atgcccatgc gctctttgat
     4141 ggccctgatc ccgagcgttc cgtcgctccc cgtcccggag agcacgatcc ctaccgcctt
     4201 tccatcctgg tcgtcggcaa ggtgccggaa gaaactgtcg atgggcatcc ggagaccgtg
     4261 cgtccggacc ggttccaggt gcccgatggt gccgtggaat agagtgaggt ccgcctgtgc
     4321 gggtttcacg tacaccgtgt tcgcctcgac cttcatgccg tcctcggtct cccgcacggg
     4381 catcctggtg aaccgccgga ggatctccgg caggagccct ttctgggcag gatccatgtg
     4441 cgtgatcagc acgaaggcta tcccggcatc cggcggcatg ttcttgaaga actgctcgaa
     4501 cgcctcgagc ccgcccgcgg atgcgccgat gcccacgatc gcgaggggtc tcttcgcctc
     4561 ttccttcccg tgctcatcct gctccatggg tctctcctct tcctgcagct aaccctctct
     4621 cctccgtccg cgcactccgg actttgcggc gcaggggggg gttctggcgt ggggggcact
     4681 gaacttcaac ccggatcacg tggtcttctc tctctcctgc tcatcccggc cggtgatatc
     4741 ctcgatggca aggaggatcc gttcggggcc ggtatccgag tggagcatcc gggcgttcaa
     4801 ccgcatcacc cggtgcccga tcacgggaaa gtcgtgctcc accagaaaac cgtcgagttt
     4861 cgtctgctgg gggaggatct cctcgagcag gctgcggagc cggggaatgt tccattgctg
     4921 gttccccagg gcatagatga ggtttccttc cgtctcgtcc cgtgtggtcc gaaacatcgt
     4981 gtaaaacgag cggtttgcgg atatgatgcg gaggtcggcg tccagcacca ggaggggctc
     5041 gtggacgatg gcgaccaggc tttcggcata catccgggcc tcctcaagag agtccttcat
     5101 cttcttgagc ggcgtcgtat cggtgaaggt gatgatgacc ccttcgatac ggttctccgt
     5161 cgtccggtag gggaggatcc gcatcagata ccactttccg tccgccgtct gcacccgctt
     5221 catctgcatc tgcagggtgt cgagcaccgt tcttacatcc cccacgatgt cttcgtccag
     5281 tatgctgata tgtaagtcgg tgatgggccg cccggtatcc cctgcctgga ggttgacgat
     5341 gggccggatg gggttggtga accgccgcac ccgcagatcg ttatcgagga agagcatggc
     5401 gatatcggtg ctctgcagga ggttgcgcat gtcgtcgttg ctctccgaga gctcctcgat
     5461 cttcctctgg tgttcggcgt tgacggtgag cagttcctcg tttaaggatt ggagttcctc
     5521 cttcgaactc gtcaactcct cgttcgtgct cttcagttcc tcgttcgtgc tctggagctc
     5581 ctcgttcatc gacttgagtt cttcctggga ggcctgcata tcctcgaccg tgcgctggag
     5641 ttgggccctg gtctctgcga gttctttatc gagatcgggc tgcgtggcag ccttccatgc
     5701 cgccccctct tcatgcccca cctccggggc ggggtcctgc accggctcga agacgaccat
     5761 gaggagatcc tctgcgccct cgatctcgcg tatcgggcgg acggtcaggc ggatagcctc
     5821 ttcgctcccg ttcgtctgca cgaagaccgg cccccgcgtg acggtgcgct tctcctggag
     5881 agcggtgcgg gtggtggagg caagcggata gcggagaccc tcccgcgcca tcgtatggac
     5941 gttcagggtg gctctgcccg gatggggttc aagatacttc ccggtcctcc cctggaagta
     6001 caggatgtcg ccgttctcgt tcacgatcac cgccggcggg gcatactgcg agagtagcca
     6061 ctcccgggcg agtgcggcga tggaggaccc ttcgttctcc ttccggagcc tgggttcgcc
     6121 caccacgagc ccgaacgagg ggatgagggt ggagggcagt tctacctgga tatcatcccc
     6181 tccgggcccc gccgcccgcc ggtagatctt ccatgtcgca tcgatggtct cgaagtgctc
     6241 caggggctcg ccggcggagt agtcgatgcc gaggatgagg atcccgccgg gattcaaggc
     6301 ataatgaaag agaggtatca gtttctgcac cacatccggg ctcgtgtaga ggagaaggct
     6361 ccggacgtcc aggatgtcca ggtggaggaa cggcggatgg gtcagtatgt tatgctgggc
     6421 aaatatggtc ttttcgcgga tctcctggcg gatctggtag gtgttgtcct gccgggtaaa
     6481 gaagcgatcc agttgctcgg gggatacatc cacctcgatg tttgcgatgt agatcccccc
     6541 ccgcgcttcg aggatgctct cccggtcgat gtcggtcgca aagatctggt actggatatc
     6601 gctgcccgag gactggatcg tctcttcgag cgcaatggcc atgctgtagg cctgctcccc
     6661 ggtggcgcac cccgagaccc agacgcggag cgtgctgtca ccgggttttg cccggatcaa
     6721 ctcgccgagc agcctctcgc ggaggatctt ccataccccg gggtcgcgga agaaccagtt
     6781 cggaccggtg gggatctcct gcgcgaggaa atcgatctct cgtgggtgtt cctggacgta
     6841 gcgcacgtag tcttcgccgt cggtgatccg gtgcagaccc atccgccgct cgatcctgcc
     6901 gcggatcagg ctctccttga atatggagta gtcttcgccc gtccgcgagc ggatcagggc
     6961 aaggatcttc gtgcgcgtat tcaggtaccg cttctcctcc gggctcgccg cgcgaaggct
     7021 ccggtaggac tggatataat tggcaagcag tttcgggagc tcgcccgccg gcgctatgta
     7081 gtcggcaagc ccggagacga tgacgctccg gggcatgctc gggaactctg cggtgatcgg
     7141 gtcctggacc atcaccgtcc cgaggcgctc ttttaacgag cggaggccga gcaccccgtc
     7201 gtgccccatg ccggagagga ggatgccgac cgcttttgag tcctggtcct gagcgagacc
     7261 gcgaaagaag tggtcgatgg gcagccgcat ccccctcggt ctggacggtt cttcgaggtt
     7321 gaggatgcct ttctggatgg tgaggttggt gtttgggggg atcacgtaga ccgagttttg
     7381 ccggaccttc atgccgtgct cgatctgtac caccggcatc gtggtgcttc gctggaggat
     7441 ctcgggcaga aggctcctgt gttcggggtc catgtgggtg accaccacga accctgcgcc
     7501 gatatcgggg ggcgtgttct tgaggaaggt ctcaagggcc tcaagccccc cggcagatgc
     7561 gccgatgccg gtgatgatga agggcggcac ttctgtgatc tcttccggtg taacggcttc
     7621 tcctggtctt gtttctgctg gatctacctc tcctttcgcc ataccacggt cgccctcatt
     7681 cctccggtcg ggatgtgagt acatctatgc acccggcatt aaacctgttg caccggggtc
     7741 cgcgcgaccg ccgtgatgcc ggtgcccgga gaccccgcct gcgatgcccg gttggctccg
     7801 gtggaggtgc acccggtgac ggcggaggcc tgcgccacgt ttgccggctc cggggatggc
     7861 gggcggagca ctacccccag atcgggtcgt cgccgaaccg gtccatccag tgcctggtgg
     7921 ggttctgccc caggccctca gcgatatctt cggtccggaa agagagcgtc tggttcatgc
     7981 agtaatcgac caggaggtcg taggcctcct tcaggcgcag ggtcatctcg tgggatttta
     8041 ccgggtcgtt ctgcgacacg tcggggtgcc actccttgat ccgatcgtga taacgtctcc
     8101 ggatctcgtt gaggctggca cggtcccgga tgccgagcac cccggccgcc tccctcacct
     8161 gctcggcggt aactgcagcc atcagggatc tgtggacgtt cccgcaggaa aaatctgccg
     8221 gagtatccag agcagcctcc tgccggccgg ggtgcccggc accgcgagac cggtacggcg
     8281 gatcagcggg attacataga ggtgcgaaga gatcctgtac catgatccgg tcttcagaaa
     8341 gacccggcac cctgaccgga acgtcggcgg tgagccaatc cgtatgcggc cagccccggg
     8401 tgaggtcatt gccatgaccg ggagctcggg ggcggctatc cggttgttcg actacggccc
     8461 gatcttcctc ctccggcact gcctgcgtga cctgcacctc gatcggcacc tccccgaact
     8521 cttcccggag accgaggccg ccatggtcgt cgcgctcgcc ttcaaccgcc ttatccggcc
     8581 ggcaacgatg ccctatatcg acacctggta tgcggagacc gccctcgccc tggacaaccc
     8641 ggccctctcc ctctccgatc gagagatcgg tgaactcctc aagaagatcg gcacgagcga
     8701 tataccggat accctggcgg acctgattat cgaggagaac gagacgcggc gagccctggt
     8761 gtacgacatc accagcctct cgggctcctc ctcgctcgag cacgggtata gccaggatag
     8821 cctgctcctg cccccgatca ccctctccct ggtcgtggat gaggaccgcg gcatcccggt
     8881 gatgtatgat gtctatccgg gcagcatcac cgatagcacc accctcgccg gcacccttgc
     8941 aaggctcgag gcctgcgggg tcacggactg caccgccatc ctggagagcg ggttcttcag
     9001 ttcggacaac ctgctggata tgctcgatga gcagatatcg ttcgttatcg ccgccaggtt
     9061 cagcatgaaa gagctcaggg cgctgatcac cagggagcag cgggatatcg cccgggccgg
     9121 gtatatccgg gagttccacg gccggacaat ccatgtcaaa ccggtggttt ttgccctcga
     9181 aggccgtgcg ttgcagggct acctgtatta cgaccccggg cttgagcagg aagcgagaga
     9241 ggcgttcacc cgccacctgg atgatatccg ggaggcgctc tttgccgtga ggctgaagcc
     9301 gggccagatc gcggatcacg tcttttccgg gatagccggg gagtttgggg gtttctttga
     9361 ctgcaagcag gtggaggacc ggatcgaggc gaccatccgc cagagcgcgg taaccctgcg
     9421 gacgaaccgg atgggaagat acatgctctt ttaccagggg aacgcggact ggatgacgtg
     9481 cctctcccgg tacagggagc gggactgtat cgaagaggag ttcgagaggc tgaagggtga
     9541 actggaggct ctccccctgg aggcgcacgg caccgccgcg ttccatggct acctcttcat
     9601 cgtgtttctc agcctgatgc tgcggtcgcg gctggcgagg atgctggagg atgcggggct
     9661 ggcagagacg tattcggtgg aaggggtgat gcttgagctg gagaagtgga agaagatcgt
     9721 cctggcggac ggccgggtta cgacgacccg gatgacccgg aaacagaaga cgatcctgaa
     9781 ggcgctcggt ctcgtgcccc tcccccgcga ggccaaagag aagggccggc cacggtaaac
     9841 cccgggtgct caccggctcc ctggattgca cgttcgcctc agccgggggc cggatatacc
     9901 ggattgcgag cggggagagt tgccgcagat ggcgttcccg gtatcccctg cctggaggtt
     9961 gacgatggcc ccggcaaatc ccgccgggga ccggcccggg tacgcggcgc tatagggagc
    10021 agccccctcc gggctgcacc aggtgatgcc tcatgtgccg gcaggatacc ccgctctcct
    10081 gattctcccg gaaggcggcg aggtcgatcc ggctcccggc aaaagtttta tctatggcag
    10141 gtgctatagg gtgctttcgc ggtaacctat gacgaaagta ttcctctgta cgaacgggtg
    10201 cgtcgagggg cagctcagga caaaattcgt cgagaggtac ctgggtgctc acgctcagaa
    10261 tatccatatg gcgtcggcga taggcgatgc cgatctcgtt ctcttctacg cgtgtggtct
    10321 gaccggcgag aaagagggcc attcgctgag cgtcatcgat gaactcaagg agaagatgcg
    10381 gcaggatgcc gaactcgtcg tctggggttg cctcccgaaa cagaaccccg gggcgcttgt
    10441 aggagtctac gacggcccga tcatcggtcc gatggatacc ggctccatta cctctcttat
    10501 aggcacgaac ggcgtctccc cggagagcat cgaccgctcg gcggccgcaa gcgagcttgc
    10561 gaccatggcc tcgcccagta tccacggctg caacggcacc gacctgctca ccgccgcgtt
    10621 cctcctctat aaacgggggg tggacctctt cggctcgcgc cggcagaggc gctccgaggt
    10681 gtatcacata cctatcgcgg ccgggtgcac cgggcattgc acctactgca gcgaacggcc
    10741 ggtctttggg aataccgtcc acagccgtcc catcgatgac atcgtatcgg agttccgggg
    10801 cgggctcgag cgtggatata gccgtttttc cctgttcgct acagacctcg ggagttacgg
    10861 catcgatatc ggttgccgcc tccccgacct cttgagagac ctggtcgcca tcggtccggc
    10921 ggagaactgt tcgatcctcc tgaaccagat cgaaccgcac cgctttgaag aggtctacgg
    10981 cgacatggag cccatcctcg catccggtaa gatcgagatg gtgatggcgc ccgtccagtc
    11041 ggggagcaac cggatcctga aactcatggg ccggcactac accgccgagc ggtggagcgc
    11101 gctgatgctg gacatctacc ggaactaccc cgctatccgg ttgagcaccc agttcatggt
    11161 ggggttcccg acggagacgg aggacgactt cgctgcaacg ctctctctcc tcgatccgcc
    11221 gctcagactc gacgaagcgc atatattcag gttctccccg cggccgacgg ttgccgcctc
    11281 ccgccttccg ggccaggttc ccgatgacgt caaggaggcc cgggctgcac gcctgctgag
    11341 caggtttgca aggaggcgta tatagccggt gagagcggac cagagcccgc tgccttccgg
    11401 gagcgtccgc ccgcccaccc gcaccgggtg gcggaccata cctctccgat aggagaggcc
    11461 ggaaagatcc cggcatttgt tcatcgccgt agcgacagga gcgggtcgtg tacgggccgg
    11521 catgtgcagc cacctccggc ccgccggttg ctcctggtcg aggatgcggc cgtgccccgt
    11581 gaccgcaacg ttattatagc ccggggctgt attgcagaat cgtcttttgg gggatggaaa
    11641 gggttcgcat gattcggaag atggcgtgta cggggttcct cgtgatcctc gttgcctctg
    11701 tgagcatggc ggttctcctc gcctttatcc aggcaggcgg catacatacc gccggtatcc
    11761 cggagaagaa cgccatactg gtcgctgccc gcgatagccc ggagtcggca aaggcgctcg
    11821 cggattacat ctgcgacggc gttgccgacc aggtggagat ccagcaggcc atcgatgcgt
    11881 cccggtcgag agggatgatc gtctctctcg cagaagggac gttcaccctt gatggagacc
    11941 tggtggtgcc cgatgatacc actatcgagg ggaagggcga taccaggacc cacctccact
    12001 ggatatccgg ccggcttact gcgtacgggg gggagaatat cgtccttaga gactttgaga
    12061 cctgcgggac cggagcgata ttcttcttca attgcagcca cgtgaaagtt cacgacgtca
    12121 ccgcacgcat cgataactcc ctcggcgggg gggcgttctt cgtctgggtg cagaacagcg
    12181 tatccgagga tatcgagttc gtcaactgta aggccatcga ctgcgggagg atggggttca
    12241 tgaacgacgg cgagggggcc cggagactca tccgggatat ccgctacatc aactgccagg
    12301 ccatcaactc gggaaggcac gaaaggttcg ccccctacgg ggagtggacg accggctttg
    12361 ccatagcgga gaacaacgac cttgccgatg cggtgctcac cggctgtctg gccgagggtt
    12421 cgtttgagtc cgggtttcac gtcgaagacg cccccacggt aacgaacctg gtattccgga
    12481 actgtatcag caggaataac ggccagaaac cggatgcctt ctacaacccc tcggaggagt
    12541 cgcacgggct ccacttcggt tcgggctact gggtgcaggg agagacgacg ctctacaact
    12601 gcgtatccga aggcaacggc aatgcgggtt tctccgcggg tcccggggtc cgcctctaca
    12661 gctgcaccga cgacggttcg gcggtggggt tccgcctggt ggagacgcat gatgtctaca
    12721 tcgaggactg caccagccgg aacgccgggg cgtatggggt ctacgcgctc gaggcggaga
    12781 ggatcgtggc ggagggcctg gagatcctcg atccggccgg tttcgacggg agcggttcgt
    12841 tcttcggcac gacggcgcac ccggtcgtcg acagccggtt tgacatcgtc ggccggaaca
    12901 gcgagaccat ccgcacgatc tacggcgaag gctgccgcga cgtcacgttc acgggcgtca
    12961 tccggaccga tcacagagag ccggtggtca tcgcgagagg ggcaggtgtc gatacccgcg
    13021 gtctgcaggt cgaggcgaaa gaggagatcg cgagccccgg ccaggatggc ggctgcgggc
    13081 tgaagaactt cctgatcagg atcatcacgg cgtttctatg aagatagtcc aggttactgc
    13141 ctactacccg ccgcacaccg ggggcatcga gcgctacgtc cataacctga gcagggcgct
    13201 tgccggccgg ggccatgacg tcaccgtcct gacggcgaac gtgccgaggg gccgcccgtt
    13261 cgagaccgag aacggggtgg cgatcgaacg ccgcccctgt ctggtgcgcc ccttcaacga
    13321 cccgtttgtg ccctcgttct tcaggcccgg cgagagcctt gccggagtcg acgtcctgca
    13381 cgctcataat gcctacagtt gcgctgcgtt gagcgcgggc gtcgtccggc ggaagttcgg
    13441 ggtgccgacg gtcctgacgc accacgggca gcatatgttc gggcatcccc tcaaggacct
    13501 cttcgtgacg ctgtaccggg ggaccatcga gcacgggctt gtccaccggg tggaccgggt
    13561 cgtcaccctc tctccctcgg atgcggcgta cgtctcggcg ttcggtgtcg gccgggagcg
    13621 gatctccgtc ataccaaacg cgatctgccc cgaggacttc ctgccctgca ccagcgagga
    13681 tacggccgga tttgaggagg cgcacaggct cgaagggaag aagcgggtgc tttttgtcgg
    13741 gcagatatcg acgaggaagg gcgtcgacgt gctcctgcgg gcggtcgacc agatcgtgca
    13801 cgagcagggc cgggacgacc tggtcttcct cttcgtcggc gacggggatt accgggaaag
    13861 agcgcaggag ctcgcccgca ggcaccgtgt cgaggagtat gtccggttcg ccgggaaggt
    13921 ggggctctgc gacctcgttg ccgcataccg gtcgtcggac gtgtatgtcc tcccctcgct
    13981 ctcggagggg atgcccacgg tgatcctgga ggccatgttc ttcggcctgc cggtcatcac
    14041 gaccgacctg ccctgtctcc gggataactt cggcgaccat gccgttctgg tgcccccccg
    14101 cgacgagcac gctctggccc gtgccgtcac ggggctcctt gacgacgccg cacgctgccg
    14161 ccggctctcg gagcggggga aggaacttgt ccggtcgaac tacacctggg ataacgtcgt
    14221 gctggcatac gagcggctct acgagcagat gaccgactga gcggtcagcc ggccccctgg
    14281 tgcggcagag gaccggcccc tccgggctgc tgccggcaga ttcttttctt gtggttgccg
    14341 tggaagcgcg ggcgtcatcg ccgtacccgg ggcccggggg ccctcgttcg tcgccgtcgt
    14401 gtccctgccg gaggcgcggg cggggcggcg ggccttcgtg aagcgccgta tgggtg
//
LOCUS       DAOSII010000043        14381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_4305_length_14381_cov_49.171018, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000043 DAOSII010000000
VERSION     DAOSII010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 14381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14381
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4305_length_14381_cov_49.171018"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..356
                     /locus_tag="PLV90_04820"
     CDS             117..356
                     /locus_tag="PLV90_04820"
                     /inference="COORDINATES: protein motif:HMM:NF040425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6444 domain-containing protein"
                     /protein_id="HOF96470.1"
                     /translation="MTAENPYPEAGVGDRPLSQYTLADIPVLLAIIGDLKRLITEQQL
                     LITELRDENARLKARIADLESQLNQNSRNSSRPNG"
     gene            553..1626
                     /locus_tag="PLV90_04825"
     CDS             553..1626
                     /locus_tag="PLV90_04825"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HOF96471.1"
                     /translation="MPHPRIIVTEHRAEHKRCPHCGCSHPTEFPAEVAHHVQYGSNLK
                     ALPVYFCIYQLLPYERASEMIFDLFGHSLSPATVVAVTGACSRNLTEVEGTTRNLLQD
                     AQVLHVDETGMRVGDTRHWLHVVSTDLLTSYGCHRKRGAQATDAMGILPAFKGTMIHD
                     FWAPYFRYTSDHAICNARLLRELQGISENDKHQWSEALGNLLIEITTAADIAKQEGRS
                     LTPEGLATFEGQYREILEAGEEETKISEIPEEQGKRGRKKQSKAKNLLDRCRKYQGEI
                     LAFAKDLPIPFTNNQAEGDLRMMKLRQKISGVFRSEEGATYFCRVRGFISTIRKHDRP
                     VLSELRRVFEGTPFMPTATHRMP"
     gene            1680..2885
                     /gene="preA"
                     /locus_tag="PLV90_04830"
     CDS             1680..2885
                     /gene="preA"
                     /locus_tag="PLV90_04830"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HOF96472.1"
                     /translation="MPAPYPSLEATVGPLTFENPFLLASGPPTASGEQIRRAFRLGWA
                     GAVTKTITPGTMEIRDVSPRFAAWKDEHSGLLGFENIELLSRRSVPYWTAEIAAIKRD
                     HPDRVLVASIMASPDIDEWQALARIVEAAGADAIELNVSCPHGMPERGVGAAIGQHPD
                     LIREVTRAVRQVTAAPLIVKLTPNVTDIMPAARAAVDGGADILSAINTVQCLMGIDLE
                     TLEPRPSVAGSSTYGGYSGPAVKPIGLRMISQIARELPVPLMGIGGISRWQDAAEYIA
                     AGASAVQVCTAVMWEGAGIIRAMNAGLSGYLAEKRFPSVGALRGRALSRIGTHAALSR
                     SVHRIAIRQFPERCASCGRCVTACRDGGYRAISIADRQVAIEKERCDGCGLCSYVCPE
                     GVIVMLPGA"
     gene            complement(3074..4288)
                     /locus_tag="PLV90_04835"
     CDS             complement(3074..4288)
                     /locus_tag="PLV90_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HOF96473.1"
                     /translation="MKKLSLFIALLLVIPAAALALSSGSMESAPTGSGLTQVATFENG
                     TRYQAGAYPVVVLSGTYREMGRQYGALMKDDLTANYAFVYASFTDRGYNVSQMNEFAR
                     TGTDLQPKRMKEIREGMAETSGLTVEELEILYMEVCVVMSAVLQTEPACSFMAVWGNY
                     TNDGSVVLSRNYDMPDVISPYYPYYTLVVYNPTDGSNGVATFNPVGARTETLMNSAGL
                     YISENNGAHAVGTMEVANRPDLIDEFFGMMLDYSDMKGLDAALMAARSDYAWIVNTAC
                     PDFAYSYEESVYDIKRLEGHEIIVAANHFDDPTWYIEGPPVVNSITRCHNLRRQAEEN
                     RGSIDGERMVEIRNVLMRNGGATFLHDDVSGDMTYSTEHQVVYSPETRTLWMKVVDRD
                     WQKVELGPLFAV"
     gene            complement(4494..5732)
                     /locus_tag="PLV90_04840"
     CDS             complement(4494..5732)
                     /locus_tag="PLV90_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-activating nucleotidase"
                     /protein_id="HOF96474.1"
                     /translation="MGDIARQAPDKDTGEDIYQYLLERITNLENRNLELREQFRQMES
                     EKRYVETQKIRYERELRKLKSEIEQLRSPPLVIGTVTDVIDNNRVIVRSSAGPRFLVR
                     TSQLIDPDLLKPGVRCTLNQQSLAIVDVLPTSYDAQIYGMELVESPEETYENIGGLEA
                     QIEEVREAVELPLTKPHLFEKVGISPPKGVLLHGPPGTGKTLLARAVAHQTNAHFLRV
                     VGSELVQKYIGEGARLVRELFELAKQKSPSIIFIDEIDAIGAHRSDSTTSGDREVQRT
                     LMQLLAEMDGFDNRGDVKIIAATNRIDILDRALLRPGRFDRMIEIPLPDHTGRLAILK
                     IHTQSMNLGEDVNLPDVARLTEGKNGADLRAICMEAGMFAIRMERDTVSADDFMKAIE
                     KLSVDFDRHHFHTTFGEMFA"
     gene            5790..6278
                     /locus_tag="PLV90_04845"
     CDS             5790..6278
                     /locus_tag="PLV90_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein bridging factor aMBF1"
                     /protein_id="HOF96475.1"
                     /translation="MQCELCGALIQGPPKTIQIEGAELCVCVRCAKHGTEVQQTRRKG
                     APQQKSGVAVPQARRKPRDVFDLMEGEIVDDYADRIRVAREGKEWSTLDLAQAIKERE
                     ILVKKIEKGDLIPEDDVRRKLEKALNIRLIDSAEDSASAGGPGRVTMTVGDVISFKKS
                     RK"
     gene            6577..7368
                     /locus_tag="PLV90_04850"
     CDS             6577..7368
                     /locus_tag="PLV90_04850"
                     /EC_number="2.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HOF96476.1"
                     /translation="MRGKEIRLERIMDRNTGRTIIVPLDHGVTLGPIPGLVDVGRTID
                     LVAEGGANAVIGHVGLALHGHRGHGRDVGLIMHLSASTSIGPDPNEKVLVNTVTNALK
                     MGADGVSVHINVGADSEARMLADLGNVAVACMEWGMPLLAMMYPRGRKVADEHDLESV
                     KLAARVAAELGADIVKTVYTGDADSFREVTRGCPVPVVVAGGSKTDDRAILDLVEGAM
                     EGGAAGISIGRNAFQHAAPDRLIRAAALIVHEGRSAEEAMEILEV"
     gene            7374..8159
                     /locus_tag="PLV90_04855"
     CDS             7374..8159
                     /locus_tag="PLV90_04855"
                     /EC_number="2.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HOF96477.1"
                     /translation="MIGKEIRLERIMDRNTGRAVIIPMDHGFTMGQIEGLRNMTGIVS
                     EVSEAGANAIVLHKGIVKGGHRKRGKDIGLIVHLSASTSMNPDPNDKVLVCTVEEAVA
                     LGADAVSIHINLGAPNESRMIESAGAVVRDCNRWGIPLLIMIYPRGKGIDSTSPQAIG
                     HCVRVAEELGADLIKTNYTGNPETFRPITAACSVPVMIAGGEKGGDLETLTTIRDAIG
                     AGAAGVCMGRNAFQRDDPGRFIGAICRVVHEGISPADALERGR"
     gene            8156..9142
                     /locus_tag="PLV90_04860"
     CDS             8156..9142
                     /locus_tag="PLV90_04860"
                     /EC_number="1.4.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase II"
                     /protein_id="HOF96478.1"
                     /translation="MKQFWVDIRPWRKDLATTAIESGANVLVVDDADRVRELGRVMTA
                     AENGDLVPGKDVFEVEIVDKAAEEEALRLSREGYVIVRTGDWTVIPLENLVAQSDRIV
                     AAVTTADEAAVALTVLEHGTAGVLLTTDDPAEIRRVAGVVAGSGAEVPLTVFEVTRIA
                     PVGMGDRVCVDTCSLLVDGEGMLVGNTSSAFLMVHPETLENPYVAPRPFRVNAGAVHA
                     YILLPGGKTAYLTDLSAGDTVLVAEHTGTTREAVVGRVKIERRPLLLVEAKAGSANVS
                     LVLQNAETIRLVREGGTAVSVANLAVGDKVLGSLMEGGRHFGIAVKETIREK"
     gene            9148..9972
                     /locus_tag="PLV90_04865"
     CDS             9148..9972
                     /locus_tag="PLV90_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOF96479.1"
                     /translation="MLAGIIGGTGQMGRFFAEVFRAAGWDVIVSGRTTPLTGRDIAGE
                     ADLVMVSVPIRATVAVIREVAPLLSEEQIFCDLTSLKAEPVGAMLASRAEVIGLHPMF
                     GPGAVSLRGQTIVATPARCRPATLEGLLSIFRDQGAAITLSTPEDHDRMMAVIQGLTH
                     FGTLAKAETIRRTGADVGETLAFTSPVYRIEMGFVGRLLAQDAGLYGDILQMNPAVPE
                     VLAAFEDAVRTLREIVESGDDEEFRTLFAENAGHYASYLHTATEETDDLINHVVNR"
     gene            9969..10763
                     /locus_tag="PLV90_04870"
     CDS             9969..10763
                     /locus_tag="PLV90_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HOF96480.1"
                     /translation="MTVLTLGPAGTFSHDLAVRLYGDDVLLLPTIRAIIGRVAAGAVK
                     GLVPIENSEAGGVGETLQGFLEFDVAITGEAYMPVRHHLAARGEPERLSVIYAHPQTH
                     EQCSVLLDSLGVEVVHTSSNAASAVAMQGDERAGAVVPEMAAVLYGLPIALRDIQNSR
                     ENTTRFVEISPAPGREAGCTKCSLLVDPKADRVGLLADLLGIFARRGINLTRIESRPS
                     RRGIGSYLFFIDLEISEGWREAKEELKRMTSVRELGCYARLEVPGV"
     gene            10760..12028
                     /gene="aroA"
                     /locus_tag="PLV90_04875"
     CDS             10760..12028
                     /gene="aroA"
                     /locus_tag="PLV90_04875"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOF96481.1"
                     /translation="MIVRVSQAGPVDAAFEAPPSKSYTHRALIAGALAAGRSRIAGPL
                     RAADTELTARGLEQLGVPVEWLPGEVTVTGCNGAFPAPGEVTIDCGNSGTTLRLLTSA
                     ALLARQAVVLTGSARMLERPVGPLAGALQALGGDVAFTGRPGFPPIRVSGSLRGGKAA
                     IDGSVSSQFVSSVLMAAPYADEDVELTLPATVASRSYLDVTADVMLRFGARLDRRGYE
                     WFRVESGAPYRGRDYRIEGDYSSASYFFAIAAVCGGKVTVRGLNPASVQGDRRFLDAL
                     EAMGCRVAAAGDAVTVERTGDLRGIDIDMSSSPDTVQTLAAVAAVAASPTTITGIAHL
                     QYKESDRVGVTADALRRMGAGITVTDDSLTITPAPLHGVLVDPHDDHRTAMAFTVLGL
                     AVGGMAIRDPGCVEKSFPGFWEALYGQGLL"
     gene            12025..13377
                     /gene="aroE"
                     /locus_tag="PLV90_04880"
     CDS             12025..13377
                     /gene="aroE"
                     /locus_tag="PLV90_04880"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOF96482.1"
                     /translation="MKIVLVGFRGTGKTSVGRLLAERLALPFFDTDTLIERRAGVSIP
                     DIFGRHGEAHFRALEREVVASLRDAEGIISTGGGAVLDPANVADLRWGGRIFLLSAPP
                     DVIQERIAGSDRPGLTNLPPAEEVQALLAARKEAYIGAADVCIDTGRRTPGEVADMVL
                     REAGISGDEKSERNTLLERFALADIGDMVDRDPGLMVCAIAGNPCAHSKSPLLYNRLF
                     AHFGMRYHYTRFEWPDAGAIVRLASLLPTKGLSVTIPFKADVMRHLDLVDSHAAAIGA
                     VNTVVRCGGRMYGHNTDWLGVRAPLAHRRGGRAVVLGAGGAAAAAVYALLSLDMDVTV
                     LARTPDAARRLAERFTCRWGGLDEFRGSDADVVVHATPVGMEPDTRSLLSASDFRAGT
                     TVFDLVYTPPETPLIRAAKEAGCETIPGTEMFVHQAVAQFRLMTGIAVTPELVREMLR
                     "
     gene            13374..14375
                     /locus_tag="PLV90_04885"
     CDS             13374..14375
                     /locus_tag="PLV90_04885"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOF96483.1"
                     /translation="MNTFGRTFRCTTFGESHGPAVGVVIDGCPPGIALAEADIQPYLD
                     RRRPGKGPLESARREPDRVEILSGIFEGRTTGAPLALVVRNRDVRSEDYDALREVFRP
                     GHADYTWQAKYDLRDHRGGGRSSGRETLARVAAGAVAVRCLAPHGISIRGRVVEVHAA
                     TEPEAMEAEIRAARDAGDSVGGIVEVTAAGCPAGLGDPVFGKLDAAIAGAMMGIGAVK
                     GVEIGEGFAAARLFGSEMNDPIERTGFSSNHAGGVLGGISTGQDIVVRLAVKPTPSIR
                     RVQRTVDISGTEREISVGGRHDPCIAPRIVPVAECMMALVILDAMLEQAKYRGFGGE"
BASE COUNT         2647 a         4469 c         4755 g         2510 t
ORIGIN      
        1 cactcaaggt cataatctcc ccattcacag gtattataga acacattatt aagaagtaac
       61 gtaactattc agcctaacca aaagatcttt ttataatggc atccaaatag aatatagtga
      121 ctgctgagaa cccttatcct gaagcgggcg ttggggatcg ccctctctct cagtacaccc
      181 ttgcggatat cccggttctt cttgccatta tcggtgatct caaacgcctc atcaccgagc
      241 aacaactcct catcacggaa ctccgtgatg agaatgctcg cctcaaggcc cggattgccg
      301 acctcgaatc ccagttgaac cagaacagtc gcaacagcag tcgccccaat ggataggttt
      361 tgtcgaccaa aaacgccacg gaagaagggt gaacgccctc cgggcggtca aaagggacac
      421 gaaggtcata accttcgtcc tgttccaaac cctgatgtgg tcatcatcca tactccttcc
      481 acctgttccc ggtgcggggc atccctggaa catgtcgaac catctgccgt agagcaacgg
      541 taggtttttg atatacccca tccccggatt atcgtgaccg aacaccgtgc agaacacaaa
      601 cggtgccccc attgtgggtg ttctcatccg accgagtttc ctgccgaggt ggcacaccac
      661 gtgcagtatg gcagcaacct gaaggcgctt ccggtctact tctgcatcta ccagctgctt
      721 ccctatgaac gagcctctga gatgattttt gacctctttg ggcactcgtt gagtccggcg
      781 accgtggttg cagtcaccgg cgcgtgctcg cgaaacctga ctgaggtcga aggcactacc
      841 cgaaacctcc tgcaggatgc acaggttctc catgtcgatg aaaccggcat gcgggtgggc
      901 gacacccggc attggctgca tgtcgtcagt accgacctcc tcacctccta cgggtgccat
      961 cggaaacgag gagcgcaagc gaccgatgcc atggggatcc tgcccgcatt caagggaacg
     1021 atgatccatg atttctgggc gccatacttc aggtatacca gtgatcatgc catctgtaat
     1081 gcccgccttc tgcgcgaact ccaggggatc tcagagaacg acaagcatca gtggagcgaa
     1141 gctctgggca acctcctgat cgagataacg acagctgcgg atatcgcaaa acaagagggg
     1201 agaagtctga cgccggaagg acttgctacc tttgaggggc agtatcggga gatcctcgaa
     1261 gcaggagagg aggagacgaa aatatctgaa atccccgagg agcagggaaa gcgcggcaga
     1321 aagaaacagt cgaaagcgaa gaacctcctg gaccgttgtc gcaagtacca gggagagatc
     1381 ctggcatttg cgaaggatct ccccattccc ttcaccaaca atcaggctga gggagacctt
     1441 cggatgatga agctgagaca gaagatttcc ggagtatttc ggagtgagga gggggctaca
     1501 tatttctgca gggtcagggg attcatatcg acgatcagga agcatgatcg cccggtatta
     1561 tcggaactga ggagggtgtt cgagggaaca cccttcatgc cgacggcgac tcatcggatg
     1621 ccctgaatag ttacaaatta taaagtgctc cagcgcaatc agaataagga gaaaactcca
     1681 tgcctgcccc ctatccctcc cttgaggcca ccgtcggccc gcttacgttt gagaatccgt
     1741 tcctgcttgc ttcgggtccg ccgaccgctt ccggcgaaca gatacggcgc gcgttccggc
     1801 tcggctgggc gggcgcggtc acgaagacca tcaccccggg cacgatggag atccgggatg
     1861 tctcgccccg ttttgccgcc tggaaggacg agcactcggg actcctcggc tttgagaata
     1921 tcgaactcct gagccggagg agcgtaccgt actggaccgc agagatcgcc gccatcaaga
     1981 gagaccatcc cgaccgggtc ctcgtcgcga gcatcatggc ctcgcccgat atcgacgagt
     2041 ggcaggcgct cgcccggatc gtcgaggctg caggggcgga tgcgatcgag ctgaacgtct
     2101 cctgccctca tgggatgccg gagcgggggg tcggtgcggc catcgggcag catcccgatc
     2161 tcatccgcga ggtgacccgt gcggtgcggc aggtcactgc ggcccccctg atcgtgaagc
     2221 tcacccctaa cgtgacggat atcatgcctg ccgcacgggc cgctgtggac ggcggggcag
     2281 atatcctctc ggccatcaac acggtccagt gcctcatggg catcgacctt gagacactcg
     2341 aaccccgccc ctcggttgcg gggtcgagca cctacggcgg atactcgggg cccgcggtga
     2401 aaccgatcgg tctacggatg atctcgcaga tcgcccggga actcccggtc cccctcatgg
     2461 ggatcggcgg catctcccgc tggcaggacg ccgccgagta catagcggcc ggggcgtcgg
     2521 cggtccaggt ctgcacggcg gtgatgtggg agggggcggg gatcatccgc gcaatgaatg
     2581 cgggcctgtc gggatacctt gcagaaaaac gctttccgag cgtcggggcc ctcaggggcc
     2641 gcgccctatc ccgcatcggg acgcatgccg ccctctccag gagcgttcac cgcatcgcaa
     2701 tcaggcaatt tcccgaacga tgtgcatcct gcggccgctg cgtgacggcc tgccgcgacg
     2761 ggggataccg tgccatatcg atagccgacc ggcaggtcgc gatagagaaa gaacggtgcg
     2821 acgggtgcgg cctctgctcg tatgtatgtc cggaaggcgt gatcgtcatg ctgcccggag
     2881 cgtgaacggg tacggctcgc ccgcggcatt gccgccgcac cggaccccgg cccccctccc
     2941 cgtcagggtc gcacgagtgt cggataggac ggaaacgtcc atatccggta ttacaaccgg
     3001 accaagaagt tcagtccgga cggcacgatt aacctgtttt tgaaaaaaaa taacccggag
     3061 tttccgttgc cggttacacc gcaaacaacg gcccgagctc cactttttgc cagtccctat
     3121 ccacgacctt catccagagc gtccgcgtct ccgggctata gaccacctga tgctccgtcg
     3181 agtaggtcat gtctcccgat acatcgtcgt gcagaaaggt cgctccaccg ttacgcatca
     3241 gaacgttccg gatctcgacc atacgctctc cgtctatcga gcccctgttc tcttccgcct
     3301 gacgccggag gttgtggcac cgcgtgatgg agtttaccac cggaggccct tcgatatacc
     3361 atgtcgggtc gtcgaagtgg tttgcggcga cgatgatctc gtgcccttcg agccgcttga
     3421 tgtcgtatac gctctcctca taggagtatg cgaagtccgg gcaggccgtg ttcacgatcc
     3481 acgcataatc cgaccgggcg gccatgaggg cggcatcgag ccccttcata tccgagtagt
     3541 cgagcatcat cccgaagaac tcgtcgatca ggtccggcct gttcgcgacc tccatggtgc
     3601 cgacggcatg tgcaccgttg ttctccgaga tgtagagccc ggcgctgttc atcaatgtct
     3661 cggtacgagc ccctacaggg ttgaaggttg ccacaccgtt gctcccgtcg gtcggattgt
     3721 agacgacgag ggtatagtac gggtagtagg gcgatatcac gtcgggcata tcatagttcc
     3781 gggagagaac caccgaaccg tcattcgtgt agttgcccca cacggccatg aacgaacatg
     3841 caggctccgt ctgcaagacc gcggacataa cgacgcacac ctccatgtag aggatctcca
     3901 gttcctccac cgtcaggccg gaggtctccg ccatgccctc ccggatctcc ttcatgcgct
     3961 ttggctgaag atccgtcccc gtccgtgcaa attcgttcat ctgcgagaca ttgtagcctc
     4021 ggtcggtgaa ggaggcgtat acgaatgcat aattggcagt gagatcgtcc ttcatcagcg
     4081 ccccatattg ccggcccatc tcccggtagg tgccggagag gacgacgacc gggtacgccc
     4141 ccgcctggta gcgcgtaccg ttctcgaacg tagccacctg cgtaagcccg ctccccgttg
     4201 gggccgattc catgctcccg gaggagagag cgagggctgc cgccgggata acaagcagga
     4261 gtgcaatgaa aagggagagt ttcttcatac agagcagtgc atgcccaaac gataaaaaag
     4321 agcatgattt atctcttccc tcccgcgcat agactccaat aatcaacgcg ataccgatcc
     4381 cggccccgca gatcggcctt gccgggccac acgaccccct ccgttcggag agaaacatcg
     4441 tcggggcatc ctcacgggag ttcgaaaaaa agtgcgtaaa aagaaaacgc ccttcaggcg
     4501 aacatctcgc cgaaggtggt atggaagtgg tggcggtcga agtccaccga gagtttctcg
     4561 atcgccttca tgaaatcgtc ggcgctgacc gtatcgcgct ccatcctgat ggcgaacatc
     4621 cccgcctcca tgcagatcgc ccggaggtcg gcgccgttct tcccctcggt gagccgggcc
     4681 acgtccggga ggttgacgtc ctcgccgagg ttcatgctct gggtgtggat cttcaagatg
     4741 gcaagccttc cggtgtggtc ggggagcggt atctcgatca tccggtcgaa ccggccgggg
     4801 cggagaaggg cgcggtcgag tatgtcgatc cggttggtcg ccgcgatgat cttgacgtcc
     4861 ccgcggttgt caaacccatc catctcggcc aggagctgca tcagcgtccg ctggacctcg
     4921 cggtcccctg aggtcgtcga gtcgctccgg tgcgcgccta tcgcatcgat ctcgtcgatg
     4981 aatatgatgg agggtgactt ctgcttcgca agctcgaaga gctccctgac caggcgggcc
     5041 ccctccccga tgtacttctg cacgagctcc gagccgacca cgcggaggaa atgcgcattc
     5101 gtctggtgcg ccaccgcccg cgcgaggagc gtcttccccg tgccgggcgg gccgtggagg
     5161 aggaccccct tcggcgggga gatgccgacc ttctcaaaga ggtgcggctt tgtcaggggg
     5221 agctcgaccg cttccctgac ctcctcgatc tgcgcttcaa ggccgccgat gttctcgtag
     5281 gtctcctccg gcgactccac cagttccatg ccgtagatct gcgcgtcgta gctcgtgggg
     5341 agcacatcga cgatggcgag ggactgctgg ttgagtgtgc accgcacgcc cggtttgagg
     5401 agatcggggt cgatgagctg cgatgtacgg accaggaacc gcggccctgc gctgcttcgc
     5461 acgatcaccc tgttgttgtc aataacgtcg gtaaccgtcc cgatcacaag aggagggctc
     5521 ctgagctgct cgatctcgct cttgagcttt cgcagctccc gctcgtagcg gatcttctga
     5581 gtctcgacat atcgtttttc ggactccatc tggcggaatt gttcgcgtag ctccaggttt
     5641 cggttttcaa ggttcgttat ccgttccagg agatactggt agatgtcttc accggtatcc
     5701 ttgtcgggtg cctgccgagc gatatctccc ataggtctcc tcgattaatt atataggtta
     5761 aaatgaatat aaagttgttt gcgagactta tgcagtgcga actatgcggc gctctcatac
     5821 aggggccacc gaaaaccatc cagatcgagg gtgcagagct ctgcgtctgc gtccgatgcg
     5881 caaaacacgg tacagaagtc cagcaaacgc gccggaaagg cgccccgcaa cagaaatcgg
     5941 gggtcgccgt cccacaggcc cggaggaagc cgcgggacgt ctttgacctc atggaagggg
     6001 agatcgtcga cgattacgcc gatcgaatcc gggtagcccg cgaagggaag gagtggtcca
     6061 ctctcgacct cgcccaggca atcaaggaac gcgagatcct ggtcaagaag atagagaagg
     6121 gagacctcat ccccgaagac gacgtccggc ggaaactgga gaaagcgctc aatatccggc
     6181 tgatcgactc ggccgaagac agcgcctccg ccggcgggcc gggccgggtg accatgaccg
     6241 tcggtgacgt catatcgttc aaaaagagcc gcaaataagg tctaccccat actttctctc
     6301 acgaggagcg gccttgcccc ggatccgcgc tgtgcgcggt attccggcat atttttatac
     6361 gaggaaacgt aacgatatac gcaatgcgtg gtgaatgttt tttcgccggt gaccgctttt
     6421 acctcggtta acggagtaac catcctcgca ggagcgacac atcagttttt aatcgccggt
     6481 acccgtgata acggagcggc agcgttctat cgctatttta ccctgtatta agccaatccc
     6541 ggagactgta ctcacgatct cgggaggact gtggttatga gaggaaaaga gattcgtctg
     6601 gaacgtatca tggatcggaa taccggcagg accatcatcg tgccgctcga tcacggcgtg
     6661 acgctcggcc ccatccccgg gctggtcgac gtcggaagga ccatcgacct cgtcgccgag
     6721 ggaggagcaa acgcggtgat cggccacgtg gggcttgcgc tgcacgggca ccgggggcat
     6781 gggcgggacg tcggcctgat catgcacctc tcggcgagca ccagcatagg ccccgacccg
     6841 aacgagaagg tgctcgtgaa caccgtcacg aatgccctga agatgggcgc cgacggcgtc
     6901 tcggtgcaca tcaacgtcgg ggccgactcc gaggcacgga tgcttgccga tctcggcaac
     6961 gtcgcggtcg cgtgcatgga gtgggggatg ccgctcctcg cgatgatgta cccgaggggc
     7021 aggaaggtcg cggacgagca cgacctcgag agcgtcaagc ttgccgcacg ggtggcggcg
     7081 gagctcggcg ccgatatcgt caagaccgtc tacaccggcg atgcggactc attccgcgag
     7141 gtgacccggg gatgcccggt gccggtcgtt gtcgcgggag gctcgaagac cgacgaccgc
     7201 gcgatcctcg acctggtgga gggtgcgatg gagggcggtg cggccggtat atcgatcggg
     7261 aggaacgcct tccagcacgc ggcgcccgac cgtctcatcc gcgccgcggc gctgatcgtc
     7321 catgaggggc ggtcggcaga agaagcaatg gagattcttg aggtgtgatg aatatgattg
     7381 gaaaagagat ccgcctggag cggattatgg acaggaacac cggtcgcgcc gtgatcatcc
     7441 ccatggatca cgggttcacc atgggccaga tcgaggggct ccgcaacatg accgggatcg
     7501 tcagcgaggt gagcgaggcc ggggcgaacg ccatcgtcct ccacaaaggc atagtgaagg
     7561 gagggcaccg gaagcgcggg aaggacatcg gcctcatcgt gcacctctcc gcgagcacgt
     7621 cgatgaaccc ggacccaaac gacaaggtac tcgtctgcac cgtggaggag gcggtcgcgc
     7681 tcggcgccga cgcggtctcg atccacatca acctcggcgc gccgaacgaa tcacgcatga
     7741 tcgagtcggc gggcgcggtg gtgcgggact gcaaccgctg ggggataccg ctcctgatca
     7801 tgatctaccc ccgcggcaag gggatcgact cgacatcgcc gcaggcgatc gggcactgcg
     7861 tccgggttgc cgaagaactc ggggccgacc ttatcaagac gaactacacc gggaacccgg
     7921 agacgttccg cccgatcacc gccgcctgct cggtgccggt gatgatcgcc ggcggcgaga
     7981 aagggggaga ccttgagacg ctcacgacca tccgcgacgc catcggggcg ggcgcggccg
     8041 gtgtctgcat gggacggaac gccttccagc gcgacgaccc cggccggttc ataggcgcga
     8101 tctgccgggt ggtgcacgag ggcataagcc cggccgacgc gctggagcgt gggcgatgaa
     8161 acagttctgg gtcgatatca ggccgtggag gaaggatctt gcgaccacgg cgatcgagag
     8221 cggtgcgaat gtcctggtcg tcgacgacgc cgaccgtgtc cgggaactcg gacgggtgat
     8281 gaccgccgcg gaaaacggcg acctcgtccc cggaaaagat gtatttgagg ttgagatcgt
     8341 cgataaagcg gccgaagagg aagctctccg gctctcccgc gagggttacg tcatcgtccg
     8401 gacgggggac tggacggtca taccgcttga aaacctcgtc gcacagtcgg accggatcgt
     8461 cgccgcggtc acgaccgccg acgaggcggc ggtcgccctg accgttctcg agcacgggac
     8521 ggcgggcgtc ctccttacga ccgatgatcc ggcggagatc cggcgcgttg ccggagtggt
     8581 cgcaggttcg ggcgcagagg tcccgctcac cgtattcgag gtaacccgga tcgcccccgt
     8641 cggcatgggg gaccgggtct gcgtggatac ctgctcgctc ctcgtcgacg gagaagggat
     8701 gctcgtcggc aacacgtcat cggcgttcct gatggtgcac cccgagaccc tggagaaccc
     8761 ctacgtggcg ccgcgcccgt tccgggtgaa cgccggcgcg gtgcatgcct acatcctcct
     8821 ccccggcggg aagaccgcgt acctcaccga cctttcggcg ggcgacacgg tgctcgtcgc
     8881 ggagcatacc ggcacgaccc gggaggcggt cgtcggcagg gtgaagatcg agcgccgccc
     8941 gctcctcctc gtcgaggcga aggccggcag cgcaaacgtg agcctggtcc tccagaacgc
     9001 cgagacgata cggctcgttc gcgagggcgg cacggccgta tcggtcgcaa acctcgcggt
     9061 cggcgacaaa gtgctcggca gcctcatgga gggcgggcgg cacttcggca tcgccgtgaa
     9121 ggagacgatc cgggagaagt gacggcgatg cttgcaggga tcatcggcgg caccgggcag
     9181 atggggcgct tctttgcgga ggtcttccgg gcggccggct gggacgtgat cgtctcgggg
     9241 agaacaacgc cgctcaccgg ccgggatatc gcgggggagg cggatctcgt catggtatcg
     9301 gtgcccatcc gcgcgaccgt cgcggtgatc cgggaggttg ccccgctgct ctcggaggag
     9361 cagatcttct gcgacctcac ctcgctcaag gccgaaccgg tcggggcgat gctcgcatcc
     9421 cgcgcggagg tgatcgggct ccaccccatg ttcgggccgg gtgccgtatc gctccggggg
     9481 cagacgatcg tcgctacgcc ggcccgatgc cgccccgcga cccttgaagg gctcctctcc
     9541 atcttccgcg accagggagc ggcgatcacc ctctcgacgc cggaagacca cgaccggatg
     9601 atggcggtga tccaggggct gactcacttc ggaacgcttg caaaagcgga gaccattcgc
     9661 cggaccggcg ccgatgtcgg ggagaccctc gcgttcacga gccccgtcta ccggatagag
     9721 atggggtttg tcggccggct cctcgcccag gacgccggac tctacggcga tatcctgcag
     9781 atgaatccgg cagtcccgga ggtgcttgca gcgtttgagg atgcggtccg gacgctccgc
     9841 gagatcgtgg agtccggcga cgatgaggaa ttccgaacgc tttttgcgga gaacgccggc
     9901 cattacgcct cctacctcca taccgcgaca gaagagacgg acgacctcat caaccacgtg
     9961 gtgaaccgat gacggtcctg accctcgggc cggccgggac gttcagccac gatctcgcgg
    10021 tccggctcta cggcgacgac gtcctcctcc tgccgaccat ccgcgccatc atcgggcgcg
    10081 tggcggccgg cgcagtaaag ggcctcgtcc cgatcgagaa ctcggaggcc ggcggcgtgg
    10141 gggagaccct ccaggggttt ctggagttcg acgtcgccat caccggggag gcgtacatgc
    10201 ccgtccggca ccacctcgcg gccagggggg aaccggagcg gctctcggtc atatacgctc
    10261 acccgcagac ccacgaacag tgctccgtcc tcctcgacag cctcggcgtg gaggtcgtgc
    10321 acacgagcag caacgccgca agcgccgtcg ccatgcaggg cgatgaacgc gccggcgcgg
    10381 tcgtccccga gatggcggcg gtgctctacg gcctcccgat agccctccgg gatatacaga
    10441 acagccggga gaacaccacc cggttcgtgg agatatcccc cgctcctggg agggaggcgg
    10501 ggtgcacgaa gtgcagcctc ctcgtggacc cgaaggccga ccgggtcggt ctccttgccg
    10561 atctgctcgg catcttcgcc cggcgcggta tcaacctgac ccggatcgag tcgcgccctt
    10621 cccggcgcgg tatcgggagc taccttttct tcatcgacct cgagatatca gaaggatggc
    10681 gggaggcaaa ggaggaactc aaaaggatga ccagcgtcag ggagctcggg tgttacgccc
    10741 gactggaggt gccgggagta tgatcgtcag ggtatcgcag gccggcccgg tcgatgcggc
    10801 gttcgaggcc ccgccctcca agagttacac tcaccgggcg ctgattgcag gagcgctcgc
    10861 cgccgggagg tcgcggatcg cagggccgct ccgggcggcc gatacggaac tgaccgcccg
    10921 cgggctggaa caactggggg tgccggtcga gtggctgccc ggagaggtca ccgtcacggg
    10981 gtgcaacggg gccttcccgg ccccgggtga ggtcacgatc gactgtggga actcggggac
    11041 gacgctgcgg ctcctcacct cggcggcgct tcttgcccgg caggcggtgg tgctgacggg
    11101 gagcgcccgg atgctcgaac gcccggtggg gccgcttgcc ggtgcgctcc aggcgctcgg
    11161 cggcgacgtc gcgttcaccg ggcggccggg gttcccgccc atccgggtct ccggcagcct
    11221 ccggggcgga aaggctgcga tcgacgggag cgtgagcagc cagttcgtct cgtccgtcct
    11281 gatggcggcc ccgtacgcgg atgaggacgt ggagcttacg ctcccggcaa cggtggcctc
    11341 gcggtcctac ctcgacgtga ccgcggacgt gatgctccgg ttcggcgccc ggctcgatcg
    11401 gcggggctac gagtggttcc gggtggagag cggcgcgccc taccggggga gggactaccg
    11461 gatcgagggc gactactcgt cggcctccta cttcttcgcg atcgcggccg tctgcggcgg
    11521 gaaggtcacg gtcagggggt taaacccggc gtcggtccag ggcgaccggc ggttcctcga
    11581 tgccctcgag gcgatgggct gccgggtggc ggcggccggg gatgcggtga cggtggagcg
    11641 gacgggcgac ctccggggca tcgatatcga tatgtcgtcg tcccccgaca ccgtccagac
    11701 gctcgcggcg gtggcggcag tggccgcgtc gccgacgacg atcaccggga tcgcgcacct
    11761 gcagtacaag gagagcgacc gcgtcggggt gacggcggat gccctccgga ggatgggcgc
    11821 gggcatcacg gtcacggatg actcactcac gatcacgccc gctcccctcc acggcgtcct
    11881 cgtcgacccg cacgacgatc accggacggc catggcgttt accgtgctcg ggctcgcggt
    11941 cggggggatg gcgatccgcg atcccgggtg cgtggagaag tcgttccccg ggttctggga
    12001 ggcgctctac gggcaggggc tgctatgaag atcgtcctcg tcggtttccg ggggaccgga
    12061 aagacgtcgg tcgggcggct cctcgccgaa cggctcgcgc tgccgttctt cgataccgac
    12121 accctgatcg agcgccgggc gggggtctcg atcccggata tattcgggcg gcacggtgag
    12181 gcacacttcc gggccctcga gcgggaggtg gtcgcgtcgc tccgggacgc ggagggcatc
    12241 atcagcaccg gcggcggggc ggttctcgac ccggcaaacg tcgcggacct gcggtggggc
    12301 ggcaggatct tcctcctctc ggccccgccc gacgtcatcc aggaacggat cgccggaagc
    12361 gaccggccgg ggctgacgaa cctcccaccg gcagaggagg tacaggcgct cctcgccgca
    12421 aggaaggagg catacatcgg tgcggccgac gtctgcatcg ataccgggag gcgcacgccg
    12481 ggtgaggtgg cggacatggt cctccgggag gccggcatat ccggggacga gaagagcgag
    12541 cggaataccc tcctcgaacg gttcgctctt gcggatatcg gggatatggt cgaccgcgac
    12601 ccgggtctca tggtgtgcgc catcgccggc aacccctgcg cccacagcaa gagcccgctc
    12661 ctctacaacc ggctctttgc gcactttggg atgcgttacc actacactcg cttcgagtgg
    12721 ccggacgcag gcgccatcgt ccgcctcgcc tccctccttc ccacaaaggg gctctcggtc
    12781 acgatcccgt tcaaggccga tgtcatgcgc cacctcgacc tcgtcgacag ccacgcggcg
    12841 gcgatcgggg cggtgaacac cgtcgtccgg tgcggcggcc ggatgtacgg ccataacacc
    12901 gactggctgg gcgtgcgggc tccgctcgcc caccggcggg gcgggcgggc ggttgtgctc
    12961 ggcgccgggg gtgcggcggc ggcggcggtc tacgccctcc tctcgctcga catggacgtg
    13021 acggtgcttg cccgaacgcc ggatgcggca cggcggctcg cggagcgctt tacgtgccgg
    13081 tggggcgggc ttgatgaatt cagggggagc gacgccgacg tggtggtgca cgcaaccccg
    13141 gtcggcatgg agcccgacac ccggagcctg ctctctgcga gcgacttcag ggcggggacg
    13201 accgtctttg acctcgtcta cacgccgccg gagacacccc tcatccgggc ggcgaaggag
    13261 gccgggtgcg agacgatacc gggcacggag atgttcgtcc accaggcggt ggcgcagttc
    13321 cggctgatga ccggtatcgc ggtcacgccg gaactggtgc gggagatgct tcgatgaaca
    13381 cgttcgggag aaccttcagg tgcacgacgt tcggcgagag ccacgggccg gcggtgggcg
    13441 tggtcatcga cggttgcccg ccggggatcg ccctcgcgga ggcggacatc cagccctacc
    13501 tcgaccggag gcggccgggg aagggtccgc ttgagtcggc gcggcgggag cccgacaggg
    13561 ttgagatcct ctcggggata tttgaggggc ggacaaccgg cgccccgctg gcgctcgtcg
    13621 tccggaaccg ggatgtccgg tcggaggact acgatgccct ccgggaggtt ttccggccgg
    13681 ggcacgccga ctacacctgg caggcaaagt acgatctccg cgaccaccgg ggcggcgggc
    13741 ggagctcggg cagagagacc cttgcccggg tcgcggccgg ggcggtggcg gtgcgctgtc
    13801 ttgcgccgca cggcatatcg atccgcggga gggtcgtcga ggtgcatgcc gcgacggaac
    13861 cggaagcgat ggaggcggag atccgtgccg cccgcgacgc gggggactcc gtgggcggta
    13921 tcgtcgaggt gacggcggcc gggtgcccgg caggcctcgg ggatccggtc ttcgggaagc
    13981 tcgacgccgc catcgccggg gcgatgatgg gcatcggtgc ggtgaagggc gtcgagatcg
    14041 gggaggggtt cgccgccgcc cggctcttcg ggagcgagat gaacgatccg atcgagagaa
    14101 ccggattttc atccaaccac gcgggcggtg tcctcggcgg gatcagcacc gggcaggata
    14161 tcgtcgtccg gctcgccgtg aaaccgacgc cctcgatacg gcgggtgcaa cggaccgtcg
    14221 atatctccgg gacggagcgg gagatctccg tcggcggaag acacgacccc tgtattgcgc
    14281 cgcggatcgt cccggtcgcg gagtgcatga tggcgctcgt catactcgat gcgatgcttg
    14341 agcaggcgaa ataccggggg tttggagggg agtagggcgg t
//
LOCUS       DAOSII010000044        14168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_4390_length_14168_cov_47.047049, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000044 DAOSII010000000
VERSION     DAOSII010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 14168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14168
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4390_length_14168_cov_47.047049"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..753)
                     /locus_tag="PLV90_04890"
     CDS             complement(37..753)
                     /locus_tag="PLV90_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOF96484.1"
                     /translation="MSAVSEADLLSRFGFYRSALYIRDIDTCVASDIHIGLAETLYRQ
                     GLHFPLHEEETLIERFEAILDRFNPAVFVLDGDIFHAFDRVSRSVVETFEAVLATLRS
                     ECEVVLVTGSHDTMLPAVAGGTVERYDRGGYTVVHGDRPVGDHGTLVIGHDHPAIEID
                     MARFPCFLYGEGVFGGKDLVVMPAYNPLSPGVTINHMQRRDFLSPILRRVDAGSLQPV
                     VEVDGETVVLPPLAGIRRFA"
     gene            complement(750..3365)
                     /locus_tag="PLV90_04895"
     CDS             complement(750..3365)
                     /locus_tag="PLV90_04895"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOF96485.1"
                     /translation="MAGQEGRSDEEIADLLDENVREWCRRQLDGRFTPPQRMAVPLIH
                     DNRNVLICSPTGSGKTLAAFLAIIDDLVVRARAGELEDTVYCLYISPLKSLANDIHKN
                     LSLPLQGIEAIARERGVRHTPIRHAVRHGDTPKAEKAKMARKPPHILNITPETLGILL
                     NSPKFRESLRTVRWVVVDEIHSLAGSKRGVHLSVSLERLEDLVKEAGGGFTRIGCSAT
                     IEPLDEVGRFLVGVGREVSIVDTRFAREFDLSLICPVPDLADTTPEDLSRHLYSTIHR
                     LVGEHENTLIFTNTRNGAERILHNLRARYPEYYTEENTGCHHGSMGAEGRLAVEEKLK
                     AGKVKAVTTSTSLELGVDMPHVDLVLQVGSPKSVAALLQRIGRAGHRLGEVVKGRIVV
                     LDRDELVECAVMLREGQRGFVDRIHIPENCLDVLAQHVFGMALDGSRRIDEMLAVFRR
                     SHCYRDLEEADLASVVRYLAGEYAGLEERHIYAKIWHDAATGTVRKRGRNARMIYFLN
                     SGTIPDEFSCDVLTRDGVFAGNLDEKYLERMNKGDVFVLGGRRYRFAYRRGGKLYVDP
                     TGDRPTIPSWFSEKLPLSFDLGLHALRFKEDMLRAMEAMGTEDLVDALLCEYPIDENS
                     ARSICAIFEQQVCYLGEEAVPTPGRIVVEEQVDRENRRRLYFFMTNYGLRFNDGFSRI
                     VAFLIARDVTTNVSVGISDTGFLVSIPLEKRADPMRLLRGIRAGECRDILKEAVGGTQ
                     LEKRVFRINAARSYMILRNYSGRKRSARRQQVSADMLISFAKRLPGFAVMRETYREVI
                     EDRFEVANIEKIVGEIGAGEIDIALTRTASPSPLAFGIATLGISDAIYAEDRLSILRE
                     FQRRVMSSIGSGAAA"
     gene            3879..4478
                     /gene="hxlB"
                     /locus_tag="PLV90_04900"
     CDS             3879..4478
                     /gene="hxlB"
                     /locus_tag="PLV90_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HOF96486.1"
                     /translation="MTDHPVKNMMRLMATKIRTIADVMSDDQIDALLNEILNANRIYT
                     MGAGRSGLVAKSFAMRLMHLGLSAFVVGETVTPAMKPGDVIIVFSGSGATKTVADISE
                     TAKEIGGRVCLITSKKDSRIGRIASCVVVIESQRDKVADESAEFEIRQMMGEHKSFAP
                     LGTIFETTAMVFADAIISHLMEITQCRPEDLQCRHANIE"
     gene            4487..5593
                     /locus_tag="PLV90_04905"
     CDS             4487..5593
                     /locus_tag="PLV90_04905"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOF96487.1"
                     /translation="MNTHRYADRVRSVEMSGIRKLFDAGGPNAINLGIGQPDFDTPGH
                     IKEAAIAAIRAGKTGYTPNAGIPELREAICGKFARENDLVYRPEQILVTAGGSEALHL
                     VMQALVNPGDRVLFADPGFVSYAALATFAGGRPEGIGLDATLHIDVERAKEQMDGARL
                     FVLNTPGNPTGAVESEESVRALVEYAGDRGVTVVSDEVYEHFIYEKKHVSAARFGEDV
                     ITINAASKTYAMTGWRVGYLAAPEDYIPECLKIHQYAQACATSIAQYAALAAYTGDQS
                     AVARMREEYRARRDLLYAGLTGLGFDFPRPEGAFYMFVPMGQTLIQRAVEAGVIIVPG
                     AAFGTRAPDHARFSYATAQENLSRAVGRIGALME"
     gene            5601..6638
                     /locus_tag="PLV90_04910"
     CDS             5601..6638
                     /locus_tag="PLV90_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOF96488.1"
                     /translation="MVKELLRKLSDAHGVTSREGNIRDIVRAELAGVVDEITEDTMGN
                     LIATKRGDGFSIMLAAHMDEIGLMVQYIDEKGFIRVVPLGGWFGPVLYCQRVVLHGTK
                     GPVKGVIGAKPPHVMKDEERKKEIKIEDMFIDVGAASAGDVADLGIEIGTPITIDREY
                     TELAGNRVTGKALDNRVGVAMLIRALQQMTSPHTVYAVFTVQEELGLKGAKVSAYTLN
                     PDCAIATDVTIPGDHPGIEKRDASVEMGKGPVLVLVSASGRGLMADPRMTAWLRETAE
                     KNNIPCQLEVGTGGNTDATIIHLERGGIPSIPLSIATRYIHSPVEVVDIADIEAGVRL
                     LVEALKGKPAL"
     gene            complement(6978..7205)
                     /locus_tag="PLV90_04915"
     CDS             complement(6978..7205)
                     /locus_tag="PLV90_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOF96489.1"
                     /translation="MAKVTIERPECISCESCWTVCPEIFEQDPADGLSSIKKEYRVND
                     NPAEGEVPDDLIDCAIEAADACPVAIIEVEG"
     gene            7317..7646
                     /locus_tag="PLV90_04920"
     CDS             7317..7646
                     /locus_tag="PLV90_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HOF96490.1"
                     /translation="MREIAWITLLFAGLLETGWALGLRYTEGFTKVGPSVATLIVMGG
                     SVYLLSRSLTSLPLGTAYAVWTGIGAVGTVIAGIILFGESRSVIRLLCIVLIVAGIVG
                     LRFFSDT"
     gene            7791..9122
                     /locus_tag="PLV90_04925"
     CDS             7791..9122
                     /locus_tag="PLV90_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOF96491.1"
                     /translation="MPGGAKRSKGLSLSAAVAFAVGNMIGAGVFVLSGLIIAIAGPSA
                     VISYIICGLAVAFSGLSYAVLASIYPEDGGGYLYARRILGPYPGFLAGFAMYISITIA
                     TAFVLLGFGIYANLLTGASLDPRYAALAGLALLAALNLRGAAEAGRVEIGLVAAKVAI
                     LAGLILVGLTHVQASDFSPFLPGGASGMLRGVAMIFFAYIGFQVVAMMGGEVKDSAKN
                     VPLATLASIGIVALIYTGVVVALLAARLPSYGGESVFDAAVVYLGPIGGVVVALAALF
                     STLSSANANILGASRVILEMASEEQIPARFARLRNGQPANSILLGAGITAILILAGNL
                     SFIVELTNVTILVAMMLVNVSALILTGRKTLVSPEKNRFVIPFGPVFPLLGAASCAAM
                     LVTLAPSVLGLGIAILLAGSLLLVIEDVPERKTIVRNIRLLLRRPVERDGG"
     gene            complement(9119..9700)
                     /locus_tag="PLV90_04930"
     CDS             complement(9119..9700)
                     /locus_tag="PLV90_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOF96492.1"
                     /translation="MDDATALMLNHVRAALPASGAHGFDHTLRVVRLAEAIGAREGAD
                     MAVLIPAAIFHDIARPVEEETGAPHEEAGARMAEAYLCSISYPADLVPGILHAIRAHR
                     YSSGVDPGTLEARVLSDADNLDAMGAVGIARTFMQAGEKGGGILDATDHIRAKLVNLR
                     GRMYTDTAREIAGKRHALLVAFLNALDDEVRGP"
     gene            10056..10460
                     /gene="tsaA"
                     /locus_tag="PLV90_04935"
     CDS             10056..10460
                     /gene="tsaA"
                     /locus_tag="PLV90_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOF96493.1"
                     /translation="MIVDLNPIGHVSSPYKEKKDAPRQGRLTDTEATIVIDDHYLPAL
                     FRVEEKKHLFVLCWFDRADRTALRATPPHNPVEHGVFATRSPNRPNPISLSLVDVIDI
                     TGNTIRVRGLEALDGTPVLDIKPYSREIDCPE"
     gene            complement(10651..12576)
                     /locus_tag="PLV90_04940"
     CDS             complement(10651..12576)
                     /locus_tag="PLV90_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOF96494.1"
                     /translation="MDVTQDSSGPLAVVPRSLFRDYREFLLTPGTIFTLVSSILLAIA
                     VLYQVSGLFIDAAPEAYADRIYLAAALVGSSFIWYSAVQGIREGDFTADIPVSLATAA
                     AIAIGQYPAAAVVAVLLLLGGLLEELVAARADRALDALARLLPDRVTVRRDGKDLFIS
                     PDEVVVGDLLIVRSGERIAVDGEVLFGAAAINQSAITGESVPAEKQAGDTVFAGTLNE
                     NGAIEVLATRVGTETTLGQIRRLVADAQEEKAPVERVLDRYAKLYTPAAIILGLLLWW
                     WSGNVLQAITMLIVFCPCVIVLATPTALVASIGNAALRGSLVKKGAAVETLAHVDTVI
                     FDKTGTLTSGELQLADVVPFDDVLETSLLGYAASAEKFSEHPVGRAIVRAAAGREIAV
                     TDPESCELLTGLGVKARTGGHAVLLGRPKLFSDMGITLPKTVLWEINARACEGMSVIV
                     VMIDHAVSGLLVFEDTLREHAQETVAALNQAGIRTVIVTGDNRETAARIAEATGISEM
                     HAEMMPEEKVGIVKRFQEAGHRVAFVGDGVNDGPALATADVGVAMGNAGTDVAIETAD
                     VVLLSDDLLKLPRIIDTSRKALRTIRQNLVFAVGVLLFAVCLTVFNILTPVTGALLHE
                     LSSIPVIANSARLIGMK"
     gene            complement(12873..13007)
                     /locus_tag="PLV90_04945"
     CDS             complement(12873..13007)
                     /locus_tag="PLV90_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96495.1"
                     /translation="MPTCSDCALYTRKTATSGECSINGPVPADRDAGRCPSRTFRPRG
                     "
     gene            13209..14165
                     /locus_tag="PLV90_04950"
     CDS             13209..14165
                     /locus_tag="PLV90_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HOF96496.1"
                     /translation="MYESIVPTVMLAADLLGEMVPMMIIGVFIAEVLVALNIAGKIAS
                     LSRPLTSFACLSEECGTSFLMAFVSPPAANAMLLDYHTKEIISQKELIIAATMNSFPT
                     VVMHWRYLLPVYVPLLGIPGLIYFGLLMLAGLVKTLVIMIVGRMTLTPAPRAPAAPAE
                     VSAVTLREALGTACTSSKKILVRLLKITVPTIVIVAFLINAGVFDRLAVMMQGAGGVF
                     PVPAEGFAIIAAQFGSFVAGASVASALLAAGDMTWQQIIVTLLVGNVLTSVTRSIRWL
                     GSSYIAIFGVRTGAEIMFVSTALRNGIMVVLVAVLAYGFGWG"
BASE COUNT         2551 a         4552 c         4398 g         2667 t
ORIGIN      
        1 tccggtatag ccccctatgg atgccggaga accgcctcac gcgaaccgcc ggatgcccgc
       61 aagcggcggg aggaccaccg tctcgccgtc gacctccacc accggctgga ggctccctgc
      121 atccacgcgg cggaggatgg gcgagaggaa gtcccggcgc tgcatgtggt tgatcgtgac
      181 gccgggggag agcgggttat acgccggcat cacgacgagg tctttccccc cgaaaacacc
      241 ctccccatag aggaaacagg ggaaccgggc catatcgatc tcgatcgccg ggtgatcgtg
      301 gccgatgacg agcgtcccgt ggtctccgac cggccggtcg ccgtggacca ccgtgtagcc
      361 gccgcggtcg tagcgctcga ccgtcccccc cgcaaccgca ggaagcatcg tgtcgtgcga
      421 tcccgtgacg aggacgacct cgcactccga gcggagggtc gcaagaaccg cctcgaacgt
      481 ctccacgacg ctccggctca cccggtcgaa ggcgtggaat atatccccgt cgaggacgaa
      541 gaccgccggg ttgaaccggt cgagtatcgc ctcgaaccgc tcgatcagcg tctcctcttc
      601 gtgaagcggg aagtggagcc cctgccggta gagcgtctcg gcaagcccga tgtggatatc
      661 ggaggcgacg caggtgtcga tatcccggat gtagagagcg ctcctgtaga acccgaaccg
      721 ggagaggagg tcggcctcgc tgaccgcgct catgccgccg ccccgctccc gatgctgctc
      781 atcacccgcc tctggaactc gcggaggatc gagagccggt cctccgcgta tatcgcgtcg
      841 gagatgccga gcgtcgctat accgaacgcg agcgggctgg gcgacgccgt ccgggtcagg
      901 gcgatatcga tctcgccggc gccgatctcc ccgacgatct tctcgatgtt tgccacctcg
      961 aaccggtctt cgatcacctc gcggtaggtc tcccgcatca cggcaaaccc ggggaggcgt
     1021 tttgcaaacg atatcagcat atcggcgctc acctgctggc ggcgggcgct cctctttctg
     1081 ccgctgtagt ttctgaggat catgtacgac cgggccgcgt tgatccggaa gacccgtttc
     1141 tcaagctgcg tccctccgac ggcctctttg agtatatccc ggcactcgcc cgcccggata
     1201 ccccggagca ggcgcatggg gtcggcccgc ttctcaagcg gtatcgatac cagaaacccc
     1261 gtatcgctga taccgaccga gacgttcgtc gtcacgtccc gcgcaatcag gaaggcgacg
     1321 atacgggaga atccgtcgtt gaaccgcaac ccgtagttgg tcatgaagaa gtagaggcgg
     1381 cggcggttct cgcggtcgac ctgctcctcg acgacgatcc ggcccggcgt ggggacggcc
     1441 tcctctccga gataacagac ctgctgctcg aagatcgcgc atatgctccg ggcgctgttc
     1501 tcgtcgatcg gatactcgca gaggagcgcg tcgacgaggt cctccgtccc catcgcctcc
     1561 atcgcacgaa gcatatcctc cttgaaccgg agggcgtgga gaccgaggtc gaacgagagc
     1621 gggagtttct ccgagaacca gctcggtatc gtggggcggt cgccggtcgg gtcgacgtag
     1681 agttttccgc cccggcggta ggcaaaccgg tagcgccggc ccccgaggac gaagacgtcg
     1741 cccttgttca tccgctcgag atacttctcg tccaggttgc cggcaaacac accgtcacgg
     1801 gtgaggacgt cgcaggagaa ctcgtccggt atggtcccgg agtttaagaa atagatcatc
     1861 cgggcgtttc gcccccgctt ccgaaccgtc ccggtcgccg catcgtgcca gattttcgcg
     1921 tatatgtgcc gctcctcgag ccccgcatac tctcccgcaa ggtagcggac gacgcttgcg
     1981 aggtcggcct cctcaaggtc cctgtagcag tgggaccggc ggaagaccgc aagcatctca
     2041 tcgatccgcc ggctcccatc gagcgccata ccaaagacgt gctgcgcgag gacgtcgagg
     2101 cagttctccg gtatgtggat acggtccaca aaccccctct gcccctcgcg gagcatcacc
     2161 gcgcactcga cgagctcgtc gcggtcgagg acgacgatcc gccctttcac cacctccccg
     2221 aggcggtgcc cggcgcgccc tatccgctgg aggagcgccg ccacggactt cggggacccg
     2281 acctggagga cgaggtcgac gtggggcata tcgacaccga gctcaagcga cgtcgacgtc
     2341 gtcaccgcct tcaccttccc ggccttgagt ttctcctcga cggcaagcct cccttccgcc
     2401 cccatcgaac cgtggtggca ccccgtgttc tcttcggtgt aatactccgg gtagcgggcc
     2461 cggaggttat ggaggatccg ctcggcgccg ttccgggtgt tcgtgaatat gagcgtattc
     2521 tcgtgctccc cgacgaggcg gtggatcgtc gagtaaaggt gccgcgagag gtcttcgggg
     2581 gtggtgtcgg caaggtcggg gaccgggcag atgagggaga ggtcgaactc ccgggcaaac
     2641 cgggtatcga cgatcgagac ctcgcgcccg acgccgacca ggaaccggcc gacctcgtcg
     2701 aggggttcga tcgtcgccga gcaccctatc cgggtaaatc cgccgccggc ctccttcacc
     2761 aggtcttcga ggcgctcaag gctcaccgag aggtggacgc cgcgcttcga acccgcgagg
     2821 gagtggatct cgtcgacgac gacccaccgg accgtccgga ggctctcgcg gaacttcggt
     2881 gagttcagga ggataccgag cgtctcgggg gtgatgttga gtatatgcgg cggttttcgc
     2941 gccatcttcg ccttctctgc cttgggggtg tcaccgtggc ggacggcgtg ccggataggg
     3001 gtgtgccgga ccccccgctc ccgtgcgatc gcctctattc cctgaagcgg caggctgagg
     3061 ttcttgtgga tatcgtttgc aagcgacttg agcggcgata tgtagaggca atatacggta
     3121 tcctcgagtt ctccggctcg tgcccggacc acgaggtcgt cgatgatagc aaggaacgcc
     3181 gcgagggtct ttcccgaccc ggtcggggag catatgagga cgttccggtt atcgtggatg
     3241 agcgggaccg ccatcctctg cggcggcgtg aaccgcccgt cgagctgtcg cctgcaccat
     3301 tcccgcacgt tctcatcgag gagatcggcg atctcttcgt ccgaacgccc ttcctgccct
     3361 gccatcttct ctcacccgct ggtatccccc gggccccgcg ccctcccgca taacgatgca
     3421 gggggcggca aacccgcgct atcaccgatc cgctccagat ggagggatat ttatcgacat
     3481 cggcacccag gggagacgta atcagagaat actaccatga aaatggaatc atgcagtatt
     3541 tctgctcccg ttatgatcag ggtgccgtgc tggactttgt atatttctca atcatcgatg
     3601 atagcctttt cgccggaagg cgcggttacg ggagacggat caggcgggag atgcaggttt
     3661 tatgccggca tttcggccat tcaagcgata taattgcgat cctccggccc tggaaaccgc
     3721 ccgcctcctg ctgcggggaa aaattctatc aggtatgact tttcatcaat gcccctcccc
     3781 cctccccggg cccctgcgcc ccccggaaat cgcccccata ccggggcctc tgggatattc
     3841 taatgtatga tctcgtgaaa cctatcagag gagctacgat gacagatcac ccggttaaga
     3901 atatgatgcg gctgatggcg accaagatca ggaccatcgc ggacgtgatg tccgatgacc
     3961 agatcgatgc gctcttaaac gagatcttga atgcaaaccg catctacacc atgggcgccg
     4021 ggcgttccgg cctggttgca aaatcgttcg ccatgcgctt gatgcacctc gggctctcgg
     4081 cgttcgtcgt cggggagacc gtgaccccgg ccatgaagcc gggagacgtc atcatcgtct
     4141 tctccgggtc cggcgcgaca aagacggttg cagacatatc cgagacggca aaggagatcg
     4201 gtgggagggt ctgcctcatc acctcaaaga aagactcccg tatcgggcgc atcgcaagct
     4261 gcgtcgtcgt catcgagagc cagcgggata aggtggccga cgagtcggcg gagttcgaga
     4321 tccggcagat gatgggcgaa cacaaatcgt tcgcaccgct cggcaccata ttcgagacga
     4381 ccgccatggt ctttgcagat gccattatat cccatctcat ggagatcacc cagtgccggc
     4441 ccgaagacct ccagtgccgg cacgcaaaca tcgagtgagg aacggtatga acacgcacag
     4501 gtatgccgac cgggtccggt cggtggagat gtcggggatc cggaaactct tcgacgccgg
     4561 ggggccgaac gcgatcaacc tcggcatcgg gcagccggac ttcgatacgc ccggccacat
     4621 caaggaggcg gcgattgccg ccatcagggc gggaaagacg gggtatacgc cgaacgcggg
     4681 tatccccgaa ctccgtgagg cgatctgcgg taagttcgcc cgcgaaaacg atctcgtgta
     4741 ccggccggag cagatcctgg tcacggcggg ggggagcgag gcgctccacc tcgtcatgca
     4801 ggcgctcgtg aacccgggcg accgcgtcct cttcgcggac cccgggttcg tctcctacgc
     4861 cgcccttgcg acgtttgccg gcgggcggcc cgaaggtatc gggctcgatg caaccctgca
     4921 tatcgacgtc gagcgggcaa aggagcagat ggacggagcc cggctctttg tcctgaacac
     4981 gccgggaaac ccgacgggcg ccgtcgagag cgaggagtcg gtccgcgccc tggtcgagta
     5041 cgcgggcgac cggggtgtca ccgtcgtctc cgacgaggtc tacgagcact tcatctacga
     5101 gaagaaacac gtcagcgcgg cccgtttcgg cgaggacgtc atcaccatca acgccgcgag
     5161 caagacctac gccatgaccg gctggcgggt cggctacctt gcggcgccgg aggactacat
     5221 acccgagtgt ctcaagatac accagtacgc ccaggcgtgc gcgacgtcga tcgcgcagta
     5281 tgccgcgctc gccgcctaca ccggcgacca gagcgcggtc gcgcggatgc gggaggagta
     5341 ccgcgcgcgg cgggacctcc tgtatgcggg tcttacgggc ctcgggtttg attttccccg
     5401 gccggaggga gcgttctaca tgtttgttcc gatgggccag acccttatcc agcgggccgt
     5461 cgaagcaggt gtcatcatcg tgccgggcgc ggcgttcggc acgcgggcgc ccgaccatgc
     5521 acgcttcagc tacgcgaccg ctcaggagaa cctctcgcgg gcggtcggga ggatcggggc
     5581 gctgatggag tgagttgagc atggtcaaag agttactcag gaagttatcg gacgctcacg
     5641 gcgtcacgag cagggaaggg aacatccgcg atatcgtcag ggcggagctt gccggcgtgg
     5701 tcgacgagat caccgaggac acgatgggga accttatcgc cacgaagcgc ggcgacggct
     5761 tttccatcat gctcgccgcc cacatggacg agatcggcct gatggtccag tacatcgacg
     5821 agaaagggtt catccgggtc gtccccctcg ggggatggtt cgggccggtg ctctactgcc
     5881 agcgggtggt tctgcacggc acgaaggggc cggtgaaggg cgtcatcggc gcgaaacccc
     5941 cgcacgtcat gaaggacgag gagcgcaaga aggagatcaa gatcgaggat atgttcatcg
     6001 acgtgggcgc tgcctccgcg ggagatgtgg cggacctcgg catcgagatc ggcaccccga
     6061 tcaccatcga ccgcgagtac acggagcttg ccggcaaccg cgtcacggga aaggcgctcg
     6121 acaaccgggt cggtgtcgcg atgctcatcc gggctctgca gcagatgacg tcgccccaca
     6181 cggtctacgc cgtcttcacg gtccaggagg agctggggct caaaggagcg aaggtgagcg
     6241 cctacacctt aaaccccgac tgtgcgatag cgaccgacgt caccatcccc ggcgaccacc
     6301 ccgggatcga gaagcgcgat gcgagcgtcg agatgggaaa aggcccggtc ctggtcctcg
     6361 tcagcgcgag cgggcgcggg ctgatggccg atccccggat gaccgcgtgg ctcagggaga
     6421 ccgcggagaa gaacaacatc ccctgccagc tcgaggtcgg gaccggcggc aacaccgacg
     6481 cgacgatcat ccacctggag cggggcggca taccgagcat accgctctcg attgcgaccc
     6541 gctacatcca ctcgccggtc gaggtggtcg atatcgccga tatcgaggcg ggcgtccggc
     6601 tccttgtcga ggcgctgaag gggaaacccg cgctctgatg ctatgggaaa acccctctat
     6661 tttttagccg gaacggaggt gcagaagagg cggcgtccgc tcccggggca gactgcggaa
     6721 gaatacgtgt gcccgagagt ttgattcttg tcccggttgc gtttcattca ttggtgcaac
     6781 gttttttttg tttacggacc ctcattgttc tgatccgtaa acctgttgca ccatgatgat
     6841 gcgccttcgg gtttgatctc cctctcaggc aaaccccccc gtagctctga aaggatatag
     6901 tctttttcat gccatcggcg taaccgctcc tcaaggccta ttttttcgtt cggcgcatca
     6961 cgggcgcccg gatcgtctca cccttcgact tcgatgattg ccaccgggca cgcatcggca
     7021 gcctcgattg cgcagtcgat aaggtcgtcg ggcacctcgc cctctgcggg gttgtcgttg
     7081 acccggtact ctttcttgat cgagctcagg ccgtcggccg ggtcctgctc gaagatctcc
     7141 gggcagaccg tccagcagga ctcgcagctg atgcattccg gtctctcgat ggtaactttt
     7201 gccattctcg ctacctctat ttcgggaatg ggtctcccgg gccttaaacg tgacggccca
     7261 tcgggaaggg ggtcgggcaa agtatatcgc ctgcccggcg aaagaggaga ggatctatgc
     7321 gggagattgc ctggataacc ctgctttttg ccgggctcct cgaaaccggc tgggcgctcg
     7381 gcctcaggta caccgaaggg ttcacgaagg tggggccgtc ggtggcgacc cttatcgtga
     7441 tgggaggcag cgtctacctc ctctcgcggt cgcttacgag tctccccctc ggaacggcgt
     7501 acgccgtctg gacgggcatc ggggcggtcg ggacggtcat cgccgggatc atcctcttcg
     7561 gcgagtcgcg gagcgtcatc cgccttctct gcatcgtcct gatcgtggcg gggatcgtgg
     7621 ggctgcggtt cttttcggat acgtaagtgc cggagagggc gccgtctcgc aatgcccggg
     7681 cccttgtttc tgcccggagg cggcagtcat ctccggcaaa ggtgcgtggg cggcacagtg
     7741 caaatcccct gccggcagcc ttatctcctg cctgccggac atagaccccg atgccgggcg
     7801 gagcaaagcg gtcgaaaggg ttgagccttt ctgccgcggt agcgtttgcg gtggggaaca
     7861 tgatcggggc cggggtcttt gtcctgagcg gcctcatcat cgccatcgcc ggtccttcgg
     7921 cggtgatctc ctacatcatc tgcggtcttg ccgtcgcctt ctcgggcctc tcctacgcgg
     7981 tgctcgcgag catctacccc gaggacgggg gcggatacct ctacgcacgg cgcatcctcg
     8041 gtccgtatcc cgggtttctc gcgggttttg ctatgtacat cagcataacg atcgccaccg
     8101 ccttcgtcct cctggggttc gggatctacg caaacctgct tacaggggcg agccttgacc
     8161 cgcgttacgc cgcccttgcc ggccttgcgc tgcttgcggc gctcaacctc aggggtgccg
     8221 cggaggcggg gcgggtcgag atcggcctcg tcgccgcgaa ggtggccatc ctcgccggcc
     8281 tgatcctggt cgggctcacc cacgttcagg cctccgactt ctcgccgttc ctccccggcg
     8341 gcgcgagcgg catgctccgg ggggtggcga tgatcttctt cgcctacata ggattccagg
     8401 tcgtggccat gatgggcggc gaggtgaagg attcagcaaa aaacgtgccg ctcgccaccc
     8461 ttgcgtccat cggcatcgtg gcgctgatat atacgggggt cgtcgttgcg ctgctcgcgg
     8521 cgcgactccc ctcgtacggg ggcgagagcg tcttcgatgc ggcggtcgtc tacctcggac
     8581 ccatcggcgg ggtcgtcgtc gctctcgcgg cgctcttctc gacgctttcg tccgcgaatg
     8641 caaacatcct cggcgcctcg cgggtcatcc tggagatggc aagtgaggaa cagatccccg
     8701 cgaggtttgc ccggctccgg aacggtcaac cggcgaactc catcctgctc ggcgccggca
     8761 tcactgccat cctcatcctc gccgggaacc tctcgttcat cgtggagctg accaacgtca
     8821 cgatcctcgt ggcgatgatg ctcgtgaacg tcagcgcgct catcctgaca gggcgcaaaa
     8881 cgctggtctc accggagaag aaccgctttg tcatcccgtt cgggcccgtc ttccctctgc
     8941 tcggcgccgc atcctgcgcg gcgatgctcg tcaccctcgc gccatcggtc ctcgggctcg
     9001 gtatcgccat cctgctggca ggctcgctcc tcctcgtcat cgaggatgtc ccggagagga
     9061 agacgatcgt ccggaatata cgcctcctgc tccgccggcc cgtggagagg gacggcggtt
     9121 aaggcccccg cacctcgtca tccagggcat tgaggaacgc gacgaggagg gcgtgcctct
     9181 tcccggcaat ctcccgggcg gtatccgtgt acatccggcc cctcaggttg acgagttttg
     9241 cccggatgtg atcggttgcg tccaggatgc cgccgccctt ctccccggcc tgcatgaacg
     9301 tccgtgcgat accgaccgca cccatcgcgt ccaggttgtc cgcatccgag agcacccggg
     9361 cttccagggt tccggggtcg acccctgagg agtagcggtg cgcccggatg gcgtggagga
     9421 tgcccgggac aaggtccgcc gggtagctga tggagcaaag gtaggcttct gccatccgcg
     9481 cccccgcctc ctcgtggggg gcgcccgtct cctcctcgac gggccgggcg atatcgtgga
     9541 agattgcggc cggtatgagg accgccatat ccgcaccttc gcgggcgccg atcgcttcag
     9601 caagacgcac gacccggagg gtgtggtcaa aaccgtgggc ccccgatgcc gggagcgctg
     9661 cccggacgtg gttgagcatc agggccgtcg cgtcgtccat acaggatgtt ggcgtccggc
     9721 aggagaaaac ggttcccgtt ccgggtgccc ccccacccgg gtaagcggca ctatcacggg
     9781 cacatatgca ccatattcca caggcatccc ccgggccgcg ctcaccggac ctctccacac
     9841 ccggcgcccc gcgggccggg aggatcccct ggcggtgcat cacgtttcga aagacttaag
     9901 gaaattctcg taactattcc tatcatgaga ctgatacata tcacctcttc cgttttggca
     9961 cggcctttcg tagcaccggg ggccgtggag accaccgccc tccacgggca ggcaggcact
    10021 gctcccggcc ggcgtgcttc tgccggaggc acaccatgat agtcgacttg aaccccatcg
    10081 ggcacgtatc ttcgccatac aaggagaaga aggacgcccc gcgccagggc cggctgaccg
    10141 acaccgaggc gacgatcgtc atcgacgacc actacctccc cgccctcttc agggtcgagg
    10201 agaagaaaca tctcttcgtg ctctgctggt ttgatcgggc cgaccggacc gcactccgtg
    10261 cgacgccccc gcacaatccg gtggagcacg gcgtgtttgc aacccgttcc cccaaccgcc
    10321 cgaacccgat cagcctctcg ctcgtcgatg tcatcgatat aacgggcaac acgatccggg
    10381 tcagggggct ggaggcactc gatgggacgc cggttctcga tatcaaaccc tactcacggg
    10441 agatcgactg cccggagtga gcggatgcgg ccgggaaccc cgatcgtctg tacgccatgt
    10501 ggatagtgcc gcgctctttg cgaggcattc cacctggcgc ccctatcagc agaaaggcgc
    10561 ccggcacccc cgggtgacct taagaacgaa cgaattttat cctgctgggt tgaaggagcg
    10621 ggatgccccg cccaaaaggc gcaagacagt tcacttcatt ccaatcaacc gtgccgagtt
    10681 ggcaatcacc gggatcgagg agagttcgtg caggagcgcc ccggtaaccg gcgtcaggat
    10741 gttgaagacg gtgaggcata cggcaaagag gaggacccct acggcaaaaa cgaggttctg
    10801 ccggatggtc cggagggctt ttcgcgatgt atcgatgatc cgggggagct tgaggagatc
    10861 atccgataag agcacgacat ccgcagtctc gatggcgaca tccgtgccgg cattgcccat
    10921 agcgacgccc acatcagcag ttgcaagcgc cggaccgtcg ttcactccat cgccgacaaa
    10981 tgccacccgg tgccctgcct cctgaaaccg cttcacgatc ccgaccttct cttccggcat
    11041 catctcggca tgcatctcgg atatcccggt cgcttccgcg atccgggcgg ctgtctcccg
    11101 gttgtcgccc gttacgatga ccgtcctgat gcctgcctga ttgagggcgg cgacggtctc
    11161 ctgcgcatgc tcccgaagcg tatcctcaaa gaccaggaga ccgctgaccg cgtggtcgat
    11221 catcacgacg atgacgctca ttccttcaca ggcccgggca ttgatctccc aaagaacggt
    11281 cttgggaagg gtgatcccca tatcggagaa gagtttcggg cgcccgagca gaaccgcgtg
    11341 cccgccggtt cgggccttca cgccgaggcc cgtcaacagt tcacaggact caggatccgt
    11401 gaccgcgatc tcccgcccgg cagcagcccg cacgatcgcc ctgcccaccg gatgctcgct
    11461 gaacttctcg gcggatgcgg cgtatcctaa caggctcgtt tcgagtacat cgtcgaaagg
    11521 aaccacatcg gcgagctgca gctccccgga tgtgagcgtc cccgtcttat cgaagatcac
    11581 ggtatcgaca tgagccagcg tctccacggc cgcccctttc ttcacaagac ttcccctgag
    11641 cgcggcattt ccgatcgagg caacgagtgc cgtcggtgtc gcgaggacga ttacgcacgg
    11701 gcagaagacg atgagcatcg tgatcgcctg caggacgttc ccgctccacc accacagcag
    11761 caatcccagg ataatggctg ccggcgtata cagtttagcg tagcgatcga gcacccgttc
    11821 gacgggcgct ttctcttcct gggcatccgc aacaagcctg cggatctggc cgagcgtggt
    11881 ctcggtcccg acccgggttg caaggacctc gattgctccg ttctcgttga gggtgccggc
    11941 gaagacggta tcccctgcct gcttctccgc cggtacgctc tccccggtga ttgccgactg
    12001 gttgatggca gccgctccaa acaggacctc cccatcgaca gcgatccgtt ccccggaacg
    12061 cacgatgaga agatcaccga caaccacctc gtcaggggaa atgaagagat ctttaccatc
    12121 acgccggacg gtcacccggt cgggcagcaa ccgggccagc gcatcaagcg cccgatcggc
    12181 acgggcggca acgagttctt caagcaaccc gccgagaaga aggagtacgg cgacgacggc
    12241 ggcggcgggg tactgcccga tcgcgattgc tgctgccgtg gcaagagaga ccgggatatc
    12301 agcggtaaaa tcgccttcgc ggatcccctg gacggcgctg taccagatga acgaagatcc
    12361 caccagagca gctgcgagat atatccggtc ggcataggct tccggagctg cgtcgataaa
    12421 gaggccggaa acctggtata agaccgcgat cgccaggagg atactgctaa caagcgtgaa
    12481 gatcgtgccc ggggtgagca ggaactctct gtaatcccga aagagagacc gggggacaac
    12541 agccagtggt ccagaagaat cttgagtcac atccatactt caatcaccgt atctgcgata
    12601 ctggaagagg tctgccgggc ggagaggagt tctcgtccgc aacccacccc ccaagtaata
    12661 aatcttgtat cggtgctcat acttaatctt atttatttgc ccgaattttc atattatttg
    12721 aggagagtcg atccgaatac atcgccggag ctccggttct ctgctgcatg gtaaccgccc
    12781 ttcaacctcg ggtgtggccc cgggcgacca tcttgtatag agagcatccg ctccagagag
    12841 gggttccacc cattcggaag cctatggcgg gactatcctc gcggccggaa cgtccgtgac
    12901 gggcaccgcc cggcatcccg gtcggcagga acagggccgt tgatgctgca ttccccgctg
    12961 gtcgcggtct ttcgcgtgta gagggcgcag tcgctgcagg tcggcataac gacctctttt
    13021 tcgcccgggg ggatataggt tgtgccggcc ggcgccctcc tgcggctcag tgctaccatt
    13081 attgtgttat atcatattta atggaatttt caagcatgga acagatcatt tattagattt
    13141 cgagatgatc ttcatcatat ctccggagag gcgcggtctt cgtggcgccg ttgccggacg
    13201 ggagagccat gtacgagagt atcgttccga cggtgatgct cgccgcagac ctgctcgggg
    13261 agatggtccc gatgatgatc atcggggtct tcatcgccga ggtgctcgtc gccctgaata
    13321 tcgccggaaa gatcgcctct ctctcccggc cgctcacgtc gtttgcctgc ctgagcgagg
    13381 aatgcggcac cagttttctc atggccttcg tctccccgcc ggccgcaaac gccatgctcc
    13441 tggactacca cacaaaggag atcatcagcc aaaaggagtt gatcattgcc gccaccatga
    13501 actcgtttcc gacggtcgtc atgcactggc gctacctcct cccggtctac gtcccgctcc
    13561 tcggcatccc cgggctcatc tacttcggcc tgctgatgct tgccggcctc gtaaagacgc
    13621 tggtcatcat gatcgtcggc cggatgaccc tcacccccgc gccgcgcgca cccgccgccc
    13681 cggcggaggt atcggccgtc accctccgcg aggcgctcgg gactgcgtgc acctcctcaa
    13741 aaaagatcct cgtgcgcctc ctcaagatca ccgtccccac catcgtcatc gtcgcgttcc
    13801 tcatcaacgc gggcgtcttc gaccggcttg ccgtgatgat gcagggcgca gggggggtct
    13861 ttcccgttcc ggcagagggg tttgccatca tcgcggccca attcgggagt ttcgtcgcag
    13921 gtgcgagcgt cgcgtccgcc ctgctcgctg ccggagatat gacctggcag cagatcatcg
    13981 tcaccctcct cgtcgggaac gtcctcacca gcgtgaccag gagcatccgc tggctcgggt
    14041 cttcctacat cgccatcttc ggggtgcgga cgggggcgga gatcatgttc gtctcgacgg
    14101 cgctccggaa cgggatcatg gtggtcctgg tcgcggtgct ggcgtacggg tttgggtggg
    14161 ggtgagag
//
LOCUS       DAOSII010000045        14058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_4431_length_14058_cov_48.931158, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000045 DAOSII010000000
VERSION     DAOSII010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 14058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14058
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4431_length_14058_cov_48.931158"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            362..943
                     /locus_tag="PLV90_04955"
     CDS             362..943
                     /locus_tag="PLV90_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOF96497.1"
                     /translation="MIDKFLEGNKRFLERDFVKAPEHYGSLVSGQHPTVLWIGCSDSR
                     VDPERITGSRAGELFVQRNIGNIVPVHDWNFATVLEYAINHLEVGDIVICGHSDCGAI
                     KGLDHEGKDAYIPLWLNNAREAKRRVDDRIQMPKTPEEEKTRLRLIEIENVGLQLEHL
                     RTYPPVMAAEKGGRVRLHGLYFDLETGELKKMF"
     gene            complement(940..1284)
                     /locus_tag="PLV90_04960"
     CDS             complement(940..1284)
                     /locus_tag="PLV90_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96498.1"
                     /translation="MAEVTVEIVGFMESECGPFPCDETRSCGLEACYPSNSLIDAIGA
                     LRDELLAQYADRVEVKMTLLDEGMPDHIRKIIEERHPPIPIVLVNGRLTSIGRVSLDL
                     IKEEIEYALEET"
     gene            complement(1313..2194)
                     /locus_tag="PLV90_04965"
     CDS             complement(1313..2194)
                     /locus_tag="PLV90_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOF96499.1"
                     /translation="MSGGTIEKIRAVETPEGTFTIRLIHDDLGSIYPGMVRYTVDILC
                     GEEPVATYAVNSYEVPPGSLVDTREAAEVLFSRVAHDVASRPHTYVRPRHLTRPLPGS
                     GAYDVVILQGSPRQFGNSARIASWCSDEAARAGFSSRVFFLHELDIRPCIGCYACYNF
                     GYCPIDDDMPGIVRALESASVIVVCTPVYTGTVPAGLKAVIDRCQWLHAREKVLGREV
                     HARGLLVAVAGQRGSEPFVCITRVVGMFMGNLGIRPAAPVLIDDLDRARDVGKVEGAE
                     DDVRAALRALLAPRDEG"
     gene            2339..3367
                     /gene="carA"
                     /locus_tag="PLV90_04970"
     CDS             2339..3367
                     /gene="carA"
                     /locus_tag="PLV90_04970"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOF96500.1"
                     /translation="MKAVLGLEDGTFVAGNGFGVEGICSGELVFTTQMTGYMEALTDP
                     GYAGQLLMFTYPLIGNYGVDRENFESPRVHARGCVVREIACVPAMQPSVAEFFRENGL
                     LGISGVDTRRLTVKTRTVGTLRASLIVGSDDGEEAVRCARVSPPAGDADLIGSVSCPA
                     PYRIAGAGKRVAVIDLGVKRSILTGLSRSGADIRVFPHTATPGDIMAAEPDALLITNG
                     PGDPGKAADAIRCAREVVGTMPVIGIGMGTQVAALALGAGTYRMKFGHRGANQPVRYR
                     DGSISITTQNHGFAVDGETLPGGCTVSYRNVNDGTVEGFEDKDLAITCVQFHPDIQFI
                     DRVYRGIP"
     gene            3367..6537
                     /gene="carB"
                     /locus_tag="PLV90_04975"
     CDS             3367..6537
                     /gene="carB"
                     /locus_tag="PLV90_04975"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOF96501.1"
                     /translation="MPRKSQIKKVLIIGSGPIQIGQAAEFDFSGSQACRALREEGVSV
                     VLVNSNPATIQTDPDTADVIYIEPLKADIVAKIIEKERPDGILSGMGGQTGLNMTVEL
                     AEMGALMGVEILGTPLEAIYRGEDREQFRDLMNAIGEPVPRSMILGSTKQIDEAIQYI
                     GLPAIIRPAYTLGGSGGGVAHTPDDMRRIIEIGLMRSRIHQVLVEESVAGWKEIEFEV
                     MRDAADTCIIVCGMENVDPVGVHTGESVVVAPILTLRDDEYQRLRNAAMKIIRALDVQ
                     GGCNIQFAYKDGDYRVIEVNPRVSRSSALASKATGYPIARVAAKIAIGLRLDEIVNPV
                     TGITPVSFEPAIDYVVAKVPRWPFDKFKSADRMLTTAMKSTGEVMAIGRTVEEAFKKA
                     LRSLDTDMRRHTNPNEIRMTLTAPTDERFGCLFDAFREGFTVREVADLTSIAPFFLEK
                     IKNIVDLERRLGTACNPGDIRAARRYGFSDEELQALTGKTADDIAALSGAPTYKMVDT
                     CAAEFPATTPYFYSTWEEECELTRNGAKKILILGSGPIRIGQGIEFDYCTVHAVMALR
                     EEEGVEVHIINNNPETVSTDADTSDRLFFEPMQLEDVMNILKKDDYYGVMVQFGGQNS
                     VNLAVPLEAEIRRLGLPTKILGTSPDAMDVAEDRDRFSRLLARLGIPSPANSSAHSEE
                     EARTMARAIGYPVLVRPSYVLGGRAMELVHDEVELESYITEAVRVSRKHPVLIDSFLR
                     SAVEIDVDAVSDGADVLIGGIMEHIEWAGVHSGDSACVIPPQSLSPSVIARVRDYTKR
                     LALGLGVVGLINIQYAVQDDVVYVLEANPRASRTVPFVAKATGIALAKIAAKVMVGRK
                     LADMGIVEPAIKYVAVKEVLLPFNKLPGVDTVLGPEMKSTGEVMGIDYDFGRAYYKAS
                     TAAENTLPTAGNIFISVTDEQKEEILPIARKLRELGLSLYGTSGTVDFLTQNGVEANL
                     VRKVQEGSPNVIDIMRSGGIRLIINTPADKASRQDHIQIMRAAVDYGIPYITTLQAAR
                     AAAMAIDAIQREEITLEPLGHYHGLA"
     gene            complement(6584..7459)
                     /locus_tag="PLV90_04980"
     CDS             complement(6584..7459)
                     /locus_tag="PLV90_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HOF96502.1"
                     /translation="MVQKTKSILALTGIVLVLCLISAGCTGTGATEPKKQISIGYVTW
                     DCAIASTNVMKQVFEEAGYDVRPVAVDAGPLFQALSRGDVDFTTTGWLPHTHAHYWEQ
                     YGSRIDYVNENIPGAARIGLVVPAYVTIDSIEEMNGVADRFGGRIVGIEPGAGVMART
                     EQAIEEYGLDYALVASSSAGMAAELRTAISNEQWVAVTGWSPHWKFGRYDLKFLDDPK
                     GAYGGAEDIVTLARQGLAADDPEAYGILSRFEWTGEDIASVMTDIEGGMPEGEAAKKW
                     IDANRDRVDAWLGKA"
     gene            complement(7866..8708)
                     /locus_tag="PLV90_04985"
     CDS             complement(7866..8708)
                     /locus_tag="PLV90_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     permease"
                     /protein_id="HOF96503.1"
                     /translation="MQPPKIPVGKAAEVLVDRIDTHLGWLLDGASDGLRFLVGGFEDI
                     LLLIPAPILILLAGILVWLVTRRDIWLAGLTVVGLLLIWNLGLWNLAMLTLALVVVST
                     VLALAIAIPLGIAAAGSETLNALLKPVLDFMQTMPPFVYLIPAVIFFGLGSVPGIIAT
                     VVFAMPPALRLTNLGIRQVPRELIEVADAFGSTPRQKLFKVQLPVALPTIMAGMNQCI
                     MLALSMTVIASMIGAGGLGYQVLVGIQRVDIGMGFEAGLAIVIIAIILDRITQNILPR
                     YGEQ"
     gene            complement(8695..9951)
                     /locus_tag="PLV90_04990"
     CDS             complement(8695..9951)
                     /locus_tag="PLV90_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HOF96504.1"
                     /translation="MWRNSVIFEKAPINGTTPVPPIVSIRGLTKVFGPDTAKTLELHR
                     SGRSKREIYEETGATLALSDISLDILPGEIFVLMGLSGSGKSTILRCINRLIEPTNGE
                     IRIGGEDIVGMSRDELRETRRRRLGMIFQSFALLPHRTVLDNVAFGLEIQGVPADERF
                     RKAEDMLDMVGLGGYGASMPGELSGGMKQRVGLARALTSDPDILLMDEAFSALDPLIR
                     REMQDELLDLQQRLGKTIIFVTHDLDEALKLGDRIALMKDGAIVQVGTPEEILTNPKN
                     AYVEKFVADVDLTRVLSASDVMRRPEPVAQCTAGPRVALHLMEEHDIQGIFVVTRQHL
                     LRGLVTLEGAAGAIREGKQMADILITDLPIVAPDASLADIISLIVESPHPVAVADDTG
                     RLRGIISRGAILAALARKEEVNRATA"
     gene            10367..11554
                     /locus_tag="PLV90_04995"
     CDS             10367..11554
                     /locus_tag="PLV90_04995"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOF96505.1"
                     /translation="MEQKKVVLAFSGGLDTSVCIPLLREHYGFDEIVTVAVDVGQPEE
                     EVIRATEKGRMLADNHYTIDAKEKYIAECIFPSIKANGSYEGYPMGTALARPLIAEEI
                     VKIAEKEGAFTVAHGCTGKGNDQLRFDFIFRSAGYKIVAPMREMNLTREWEICYAQEH
                     NIPVSVGKENPYSVDENCWSRSIEGGRLEDPAFHPPEEIYAWTVSPQAAPDAVEKIRI
                     EFEQGVPVALNGERLPGIAFIRELNRIAGRNGVGRNDMIEDRILGLKAREIYEHPAAT
                     VLLAAHRDLERLVLTRSELAFKHIVDDKWSELAYMGLVHEPLFYALNAFVDTTQERVN
                     GAVDIALYKGGLSVLGRSSDAGLYSGDLVSFDSTTIDQNHAVGFSNYFGLQARLLKNS
                     KKC"
     gene            complement(11563..11967)
                     /locus_tag="PLV90_05000"
     CDS             complement(11563..11967)
                     /locus_tag="PLV90_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOF96506.1"
                     /translation="MVALSSEIKEIFTKTKVMPMATASKAGIPNVAPMASILLVNDDT
                     VWIMDNYMVKTLENLKENPVVALYFYDQDTKRCFQIKGSTEIKTSGPDYEKFRDQVKA
                     KGHKYPAKSLIVIKITDVFECTPGKDAGKKVL"
     gene            12451..13947
                     /locus_tag="PLV90_05005"
     CDS             12451..13947
                     /locus_tag="PLV90_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 14 protein"
                     /protein_id="HOF96507.1"
                     /translation="MCARFLERFFKPTPHIVESPPPPSISHGPGEGVPEYRVKPYFIV
                     ASVEMGNTTTKCILTGTNLETGRSYVINKTVTMSRDVRQPRPGETIFGETLDGTELTR
                     ESVTDLVRDTLIQCHNEAHLSIKDDLDFVVRSTGVVAAMDSPDQVGDFVIALANGCLD
                     AGVPPRKMTPPMSIDNLPPKLRQFSFADRVVFVGAVAGVVPPVGCTGVEMVANEMEGE
                     LAMAGIKEGAKWTAVDFRNPCISIDFGTTLDGRITSDVARDDPNPFAQTVGNFCGLAG
                     AIPDAIVRGTGLVKGRTGTALDLFGDHSIKGMFGGRKRSVVEEYVDRCHEHIDIRIVP
                     PDRTRFGRVPVCAEVADKSGVALIGCDTGVNGSETPALEAIGHEIYEKHGMGMLTEVV
                     DRVCAKMALRLIDVAIARGHVPPNSSIGFTGRAAISGRKPEYILEGITERNLYDTPND
                     HLVFVDDGLARGAALMGRCMNSLGKPKAPLGGVRGGPCIMSRRIKIGK"
BASE COUNT         2706 a         4341 c         4402 g         2609 t
ORIGIN      
        1 tcccgtcgca taacggtatc ctgcagggat ctccctcccc tggcgggagc gcatataccc
       61 cggcggtatt gcgattcgac ggatgacaat ccgttataaa caactccgaa ttgtcgacca
      121 tgcactgttc agtgccctga tgtacatggt tctccgtacc ccgatccgat cgtcgatatc
      181 ctgggccgat ctgctcttta gggcatcatc cgaacctgga atcccccttt ctatccggat
      241 tccgcaccgc gcctctcgcc gcaacttcca ggtatatatc tttttaagac tgggggacaa
      301 ccccattttt ggaggcgcac ccatcaggat ggccgcccct atcgtctacg gagcatcacg
      361 aatgatagat aagtttcttg agggtaacaa acggtttcta gaaagagatt ttgtcaaagc
      421 gcctgaacat tacggttcgc tggtatcagg ccagcacccg acggtcctct ggatagggtg
      481 ctcggactca cgcgtcgacc cggaacgcat caccggctcc agggccggag aactctttgt
      541 ccagcgcaac ataggcaaca tcgtcccggt ccacgactgg aacttcgcga ccgtgcttga
      601 gtacgcaatc aaccacctcg aggtcgggga tatcgtcatc tgcggacact cggactgcgg
      661 cgccatcaag gggctcgatc acgagggcaa agacgcctac atcccgctct ggctcaacaa
      721 cgcaagagaa gcaaaacgcc gtgtcgacga caggatccag atgccgaaaa cccccgaaga
      781 agagaagacc cggttgcggc tcatcgagat cgagaacgtc ggcctgcagc ttgagcacct
      841 ccgcacctac cccccggtca tggctgcgga aaaaggaggg cgggtccgcc tccacggcct
      901 gtacttcgat cttgagacgg gagagttaaa gaagatgttt taggtctctt caagcgcata
      961 ctcaatctct tcttttatca ggtcgagcga gaccctcccg atcgacgtaa gccgcccgtt
     1021 gacgaggacg atcggtatgg gcggatgacg ctcctctatg atcttcctga tatggtcggg
     1081 catcccctca tcgagcagtg tcatcttcac ttcgacccta tccgcgtatt gggcgaggag
     1141 ctcgtcgcgc agggcgccga tggcgtctat caggctgttt gacgggtaac atgcctcaag
     1201 gccgcaggat ctcgtctcgt cacagggaaa cgggccgcat tcggattcca tgaacccgac
     1261 aatctcgaca gtcacttctg ccatgcatca tcggtggggg ggcaggggta tattaccctt
     1321 cgtcccgggg agcgagcagc gcgcgcaacg ccgcccggac atcgtcttcc gccccctcga
     1381 ccttcccgac gtcccgggcc cggtcgagat cgtcgatcag caccggggcg gccgggcgga
     1441 tgcccaggtt ccccatgaac atgccgacca cgcgggttat acagacaaac ggctcgctcc
     1501 cccgctgccc cgcgacggcg acgagcaggc ccctggcgtg cacctccctc cccagcacct
     1561 tctcccgggc atggagccac tggcagcggt cgatcacggc cttcaggccg gccggcacgg
     1621 tgccggtgta gaccggcgtg cagacgacga tgaccgacgc ggactcaaga gcccggacga
     1681 tcccgggcat atcgtcgtcg atcgggcagt aaccgaagtt gtagcaggca taacacccga
     1741 tgcacgggcg gatgtcgagc tcgtgcagga agaagacccg ggaggagaaa cccgcccgcg
     1801 cggcctcgtc gctgcaccac gacgcgatcc gggccgagtt cccgaactgg cgcgggctcc
     1861 cctgcagtat gacgacgtca taggcgccgc tgccggggag cgggcgcgtg aggtggcggg
     1921 ggcggacgta ggtatgagga cgggacgcca catcgtgcgc gacccgtgag aagaggacct
     1981 ccgccgcctc ccgggtatcg acgagagacc cgggagggac ctcgtaggag ttgacggcgt
     2041 aggtggcgac cggttcttcg ccgcagagga tatcgacggt ataccggacc atgcccgggt
     2101 agatgctccc gaggtcgtca tggatgagcc ggatggtgaa cgtcccctcc ggcgtctcga
     2161 ccgcgcgaat cttctctatt gtacccccgc tcatcgcgcg ccgcccccta tggtcccggc
     2221 cccggtattc cgggcgataa aaacccccat ttccgggttt cctgcgggat ttccggagaa
     2281 cacatccgat ccgtcaccac ggttattaag gaatccgttc cacatgatat ggtaattcat
     2341 gaaggcggtc ctgggtcttg aagatggaac atttgttgca gggaacggct ttggcgttga
     2401 agggatatgt tccggtgaac tcgtctttac cacgcagatg accggttaca tggaggcgtt
     2461 gacggacccc gggtatgccg ggcagctact gatgttcacg tatcctctca tcgggaacta
     2521 tggtgtggac cgggagaact tcgagagccc gcgtgttcat gcacggggct gcgttgtgcg
     2581 cgagatcgcc tgcgtgcccg ccatgcagcc atcggttgca gagtttttca gggagaacgg
     2641 cctcctcggt atatcgggcg tcgatacccg cagactgacg gtaaagacca ggacggtcgg
     2701 cacgctccgc gcatcgctca tcgtcggcag cgatgacgga gaggaggctg tccggtgcgc
     2761 ccgggtctct cccccggcag gtgatgccga cctcatcggg agcgtctcct gccctgcgcc
     2821 gtaccgtatc gccggcgccg ggaaacgggt cgccgtcatc gacctcggtg tgaaacggag
     2881 tatccttacc ggcctcagcc gctccggcgc cgatatccgc gtcttcccgc acaccgccac
     2941 gcccggcgat atcatggcgg cggagcccga tgcgctcctc ataacgaacg gccccggcga
     3001 cccggggaag gcggccgacg ccatccggtg cgcccgggag gtcgtcggca cgatgccggt
     3061 catcggcatc ggcatgggca cccaggtggc cgctctcgcg ctcggtgccg ggacttaccg
     3121 gatgaagttc ggccaccggg gggcaaacca gccggtccgt taccgggacg ggagcatatc
     3181 catcaccacc cagaaccacg gttttgcggt ggacggagag accctgcccg ggggatgcac
     3241 cgtctcgtac cggaacgtga acgacggcac ggtcgaaggg ttcgaggata aagacctcgc
     3301 catcacctgc gtccagttcc acccggacat acagttcatt gaccgcgtat acagggggat
     3361 accgtaatgc cgcgcaaatc ccagatcaag aaagtcctca tcatcggttc agggcccatc
     3421 cagatagggc aggcggccga gttcgacttc tccgggtcgc aggcctgccg ggcgctccgg
     3481 gaggaggggg tcagtgtcgt cctcgtgaac tctaaccccg cgacgatcca gaccgacccc
     3541 gatacggcgg acgtcatcta tatcgagccc ctgaaggccg atatcgtagc aaagatcatc
     3601 gaaaaagaga gaccggacgg tatcctgagc ggcatgggcg gccagaccgg cctcaacatg
     3661 acggtggaac tcgcggagat gggcgcactt atgggcgtgg agatcctcgg gacgccgctt
     3721 gaggcgatct accggggaga agaccgcgag cagttccgcg acctgatgaa cgctatcggg
     3781 gagcccgtcc cccggagcat gatactcggg agcacaaagc agatcgacga ggccatccag
     3841 tatatcggtc tcccggcgat catcaggcct gcctacaccc tcggcggctc aggcggcggt
     3901 gttgcgcata cgccggacga tatgcggcgg atcatcgaga tcgggcttat gcgctcccgg
     3961 atccaccagg tgctggtgga agagagcgtc gccgggtgga aggagatcga gttcgaggtg
     4021 atgcgggatg cagccgatac ctgcatcatc gtctgcggca tggagaacgt cgacccggta
     4081 ggcgtccata ccggcgagag cgtcgtcgtt gcgcccatcc tcaccctgcg ggacgacgag
     4141 taccagaggc tccggaacgc tgcgatgaag atcatccggg ccctcgacgt ccagggcggg
     4201 tgcaacatcc agttcgccta caaagacggc gactaccggg tcatcgaggt gaacccccgg
     4261 gtctcccgct cctccgcgct cgcatccaag gcgaccggct acccgatcgc ccgggtggcg
     4321 gcaaagatcg ccatagggct ccgcctcgac gagatcgtga atcccgtgac cggcatcacc
     4381 cccgtatcgt ttgagcccgc catcgactac gtcgtcgcga aagtgccccg gtggccgttt
     4441 gacaagttca agtccgcgga ccggatgctc accacggcga tgaagagcac cggcgaggtg
     4501 atggctatcg gccggacggt cgaggaggcg ttcaagaagg cgctccggtc gctcgatacc
     4561 gatatgcggc ggcacacgaa cccaaacgag atccggatga ccctcacggc cccgaccgac
     4621 gagcggttcg ggtgcctctt cgacgccttc cgggagggat tcacggtcag ggaggtcgcc
     4681 gacctcacct ccatcgcgcc gttcttcctt gagaagatca agaacatcgt ggacctcgag
     4741 cggaggctcg gaaccgcctg taatcctggg gatatacggg ctgcccggcg ctacgggttc
     4801 tcggacgaag aattgcaggc tcttaccggg aagacggccg acgatatcgc ggctctttcc
     4861 ggggcgccga cctacaagat ggtggatacc tgcgccgctg agttcccggc caccacgccc
     4921 tacttctact cgacctggga ggaggagtgc gaactcaccc ggaacggtgc caagaagatc
     4981 ctgatactcg gttcggggcc gatccggatc ggccagggga tcgagttcga ctactgcacc
     5041 gtccacgcgg tgatggcgct ccgcgaggag gaaggagtag aggtccatat catcaacaat
     5101 aaccccgaga ccgtctcgac cgacgccgac acgtccgacc ggctcttctt tgagccgatg
     5161 caacttgaag atgtcatgaa catcctcaag aaggacgact actacggcgt catggtccag
     5221 ttcggcggcc agaactcggt gaacctggcc gtgccgctcg aggccgagat ccggcgcctc
     5281 ggccttccga cgaagatcct cggcacgtcg cccgacgcca tggatgtcgc ggaagaccgc
     5341 gaccggttca gccggctcct tgcccggctc ggcataccga gcccggcaaa cagctccgct
     5401 cactcggagg aggaggcgcg aacgatggcc cgggcgatcg gctacccggt cctggtccgg
     5461 ccgtcctacg tcctcggcgg gcgggcgatg gagctcgtcc acgacgaggt cgagcttgag
     5521 agttacatca cggaggccgt ccgcgtgagc cggaaacacc cggtcctgat cgactccttc
     5581 ctccggagcg ccgtcgagat cgacgtcgat gcggtctctg acggggcgga tgtcctgatc
     5641 ggcggcatca tggagcatat cgaatgggcc ggcgtccact ccggcgactc ggcctgcgtc
     5701 atcccgccgc agtctctctc gccctcggtg atcgcgcggg tgcgcgacta cacgaagagg
     5761 ctcgccctcg gccttggcgt cgtcggcctg atcaacatcc agtatgccgt ccaggacgac
     5821 gtcgtctacg tgctcgaggc aaatccgcgc gcgagccgga cggtgccgtt cgtcgcgaag
     5881 gcgacgggga ttgcgcttgc aaagatagcg gcgaaggtga tggtggggag aaagcttgcc
     5941 gatatgggca tcgtcgagcc cgcgatcaag tacgtcgcgg tgaaggaggt tctcctcccc
     6001 ttcaacaaac tccccggcgt cgacaccgtg ctcggtcccg agatgaagag caccggcgag
     6061 gtgatgggga tcgattacga cttcggccgc gcctactaca aggcgagcac cgccgcggag
     6121 aacaccctgc cgacggcagg aaacatattc atatcggtga cggacgagca gaaagaagag
     6181 atcctgccga tcgcgaggaa gctccgggaa ctcggcctct ccctctacgg cacgagcggg
     6241 acggtcgact tcctcaccca gaacggcgtc gaggcaaacc tcgtgcggaa ggtccaggag
     6301 ggttctccaa acgtcatcga catcatgcgt tccggtggaa tacggctgat catcaacacc
     6361 ccggcggata aggcctcccg gcaggaccat atccagatca tgcgggccgc cgtcgattac
     6421 ggcatcccct acatcacgac gctccaggcc gcgcgggccg ctgcgatggc gatcgatgcc
     6481 atccagcgcg aggagatcac cctcgagccg ctcgggcact accacgggct ggcgtgatca
     6541 gggggtatca ccctcctccg gaccggggtg aaaagagaga gggttatgct tttccaagcc
     6601 aggcatcgac cctgtcgcgg ttggcatcga tccacttctt cgcggcctct ccttcgggca
     6661 tgccgccctc gatatcggtc atcaccgagg caatatcctc gcccgtccac tcgaaccggc
     6721 tcaggatgcc gtaggcttcc ggatcgtcgg cagcaagacc ctgccgcgcg agggtcacga
     6781 tatcttcggc cccgccgtag gctcccttcg ggtcgtcgag gaacttgagg tcgtagcggc
     6841 cgaacttcca gtgcggcgac catcctgtca ccgcgaccca ctgctcgttg ctgatcgcgg
     6901 tgcgcaactc agcggccatg ccggcactgc tgcttgcaac cagcgcgtag tcgaggccgt
     6961 actcctcgat ggcctgctcg gtccgggcca tgaccccggc tccgggttct atgccgacga
     7021 tcctgccgcc gaaccggtct gcaacgccgt tcatctcctc gatagagtcg atggtcacgt
     7081 aggccgggac gacgagcccg atacgtgccg ctccggggat gttctcgttg acgtaatcga
     7141 tccggctgcc gtactgctcc cagtagtgcg catgggtgtg cgggagccat ccggtcgtcg
     7201 taaagtcgac gtctcccctg ctaagcgcct ggaagagcgg ccctgcgtcg accgcgaccg
     7261 gcctgacgtc gtagccggct tcttcaaaga cctgtttcat gacgtttgtg cttgcgatgg
     7321 cacagtccca ggtgacgtag cctatgctga tctgtttttt gggttcggtt gcccctgtac
     7381 ccgtgcaacc ggcggatata agacagagta ccagaacgat acctgtcagt gcgagtatac
     7441 ttttggtctt ttgaaccatg gatcacctcg taggtttttg ccgccgtccg gcggcttgat
     7501 aacgttcagg gcccggcaat atggggttcc gggctctcac gacatgtccg aaacgacaga
     7561 aggccgctgg atcggacatc gggcagagat ggcgatatgc gccactcctg gtgtgtttgc
     7621 accccaggca aaaaggccag acattgaccg cttgagcgta tgacattacc attatttagg
     7681 ttttacgggt tccgtttggc ggacggagcc tcaataaatc gaattttgag cgtcatgccg
     7741 ccggaaaccg tccccggtcc cccggcaagg gcgccgatct gccgtcgccg ggagtcctga
     7801 tggaactgcg gcggatccgg ggcggacagc cgggttgacg tgccccgggc aggggccggc
     7861 aagggtcact gttccccgta tcgcggcaga atgttctggg tgatccggtc gaggatgatc
     7921 gcgatgatga cgatggcaag ccccgcctca aaccccatgc cgatatccac ccgctggatg
     7981 cccaccagca cctggtagcc aagccctccg gccccgatca tcgacgcgat gacggtcatc
     8041 gagagggcga gcatgatgca ctggttcatc ccggccatga tggtggggag cgcgacaggg
     8101 agctgcactt tgaagagctt ctggcggggc gtggaaccga aggcatccgc gacctcgatc
     8161 aactcccgcg gcacctgccg gatgccgagg ttcgtgaggc gcagcgccgg aggcattgca
     8221 aagaccaccg tcgcgatgat gccgggaaca ctcccgaggc caaagaagat cacggcgggg
     8281 atgaggtaga cgaacggggg catggtctgc atgaagtcga ggaccggttt gaggagcgca
     8341 ttcagggtct cgctccctgc agccgcgatc ccgagcggga ttgctatcgc gagcgcgaga
     8401 accgtcgaga cgacgacgag cgccagggtg agcatggcga gattccagag gccgaggttc
     8461 cagatcagga gcagaccgac gacggtcagc cctgcaagcc atatgtcgcg ccgggtgacg
     8521 agccagacca ggatgcctgc aagaaggatc aggatgggtg ccggtatgag gagaaggata
     8581 tcctcaaacc cgcccacgag gaaccgcagc ccatcacttg cgccgtcgag gagccagccc
     8641 aggtgggtgt cgatccggtc gacgagcacc tcggcagcct tccccacggg gatcttaggc
     8701 ggttgcacgg ttcacctctt ctttccgcgc cagggcggcg agtatggcgc cgcgcgatat
     8761 gatgcccctg agcctgccgg tatcgtccgc caccgccacg gggtgggggc tctcgacgat
     8821 cagcgatatg atgtcggcca gcgatgcgtc gggagccacg atgggaagat cggttatgag
     8881 gatatcggcc atctgctttc cctcccggat ggccccggca gcgccttcga gcgtcaccaa
     8941 tccgcggagc aggtgctggc gggtgaccac gaatatcccc tggatgtcgt gctcctccat
     9001 gaggtgcagg gccaccctcg gccctgcggt gcattgggca accggttcgg gacggcgcat
     9061 cacgtcgctt gccgagagca ccctggtcag gtccacatcg gcgacgaact tctcgacgta
     9121 ggcgttcttg gggttcgtca ggatctcctc cggcgtcccg acctgcacga tcgcgccgtc
     9181 tttcatcagg gcgatacgat cgccgagttt cagggcctca tccagatcgt gagtcacgaa
     9241 gatgatggtc ttaccgagcc gttgctggag gtccagcagt tcgtcctgca tctccctgcg
     9301 gataagaggg tcaagagcgc tgaatgcctc gtccataagg agtatatccg ggtcgctcgt
     9361 gagtgcccgg gcaagcccga cccgctgctt catgcccccg gagagctcgc ccggcatgct
     9421 tgccccgtag ccgccgagcc ccaccatatc gagcatatct tccgccttcc ggaagcgctc
     9481 atctgccggg acgccctgga tctcaagccc gaaggcaacg ttgtcgagca ccgtccggtg
     9541 cgggaggagt gcaaagctct ggaagatcat gcccagcctg cgccgccggg tctcccgcag
     9601 ctcatcccgg ctcatcccga caatatcctc gccgcctatc cgtatctcgc cgtttgtagg
     9661 ctcgatgagc cggttgatgc agcgcaggat cgtcgatttt ccgctcccgg agagtcccat
     9721 aaggacgaat atctcaccgg gcaggatgtc gagggatatg tcgctgaggg ccagtgtcgc
     9781 gccggtctct tcgtatatct cccgtttgga gcggccggat ctatggagtt cgagcgtttt
     9841 tgcagtgtcc gggccgaaca cctttgtcag gcccctgatg ctgacgatcg gcgggacggg
     9901 agttgtgccg tttatgggtg ccttctcaaa aatgacggaa ttcctccaca ttcagggttt
     9961 cgatctaatc tcttttataa tttcgccttt ttaaaaatcc acgatatata cacgaattct
    10021 ggtttttccg ggattatgcc gtgcttttgg gaatagactg ccacttcatc tgtaatctcg
    10081 ttattcaggg ttattttgtc gcctttgccc cgtataatgg ccgatttgac gggatctctc
    10141 ttcgcctgcc acggcatcgg aaaaccggct gcgtcgaagg gcgccggcac ccgcctccct
    10201 catcaatgcc cctgcacgcc cggacccggg gaattatacg gatgaagtct gattcctgcc
    10261 ggtgcgctca tccataccgc ttcttttatc gggtgaaagg tcatatcaat gtagattatt
    10321 ccagactgtc agcagaacag gtatcaggaa cgcggagatt attaccatgg aacagaaaaa
    10381 ggtcgtcctg gcattttcgg ggggtcttga tacctccgtc tgtatccccc tcctgcgcga
    10441 gcactacggg tttgacgaga tcgtcaccgt tgccgtcgac gtgggccagc ccgaggagga
    10501 ggttatccgg gcaacagaga agggccggat gcttgcagat aaccactaca ccatcgatgc
    10561 gaaggagaag tacatcgcgg agtgcatctt cccctcgata aaggcgaacg ggtcatacga
    10621 gggttacccg atgggcacgg cactcgcccg cccgctgatc gccgaggaga tcgtgaagat
    10681 cgcagagaag gaaggcgcct ttacggttgc gcacgggtgc accggaaaag gaaacgacca
    10741 gctccggttc gacttcatat tcaggtccgc aggctataag atcgtcgcac cgatgcggga
    10801 gatgaacctg acccgcgagt gggagatctg ctacgcacag gagcacaaca taccggtgag
    10861 cgtcgggaag gagaacccct acagcgtcga cgaaaactgc tggagccgga gcatcgaggg
    10921 cggcaggctc gaggacccgg ccttccaccc gcccgaggag atctacgcct ggacggtatc
    10981 gccgcaggcg gcccccgacg ccgtggagaa gatccggata gagttcgagc agggcgtccc
    11041 ggtcgccctc aacggagaga ggctccccgg catcgccttt atccgggaac tgaaccggat
    11101 agccggcagg aacggcgtgg gccgcaacga tatgatcgag gaccggatcc tcggcctgaa
    11161 ggcccgcgag atctacgagc acccggctgc gaccgtcctc cttgccgcgc accgcgacct
    11221 cgaacgcctc gtcctgaccc gctcggagct tgcgttcaag cacatcgtcg acgataagtg
    11281 gtccgagctc gcctacatgg ggctcgtgca cgaaccgctc ttctacgcgc tcaacgcctt
    11341 tgtcgatacg acgcaggagc gcgtgaacgg cgctgtcgat atcgccctct acaagggcgg
    11401 cctctcggtg ctcggccgga gttcggatgc cggcctctac tcgggcgacc tcgtctcgtt
    11461 tgactcgacg acgatcgacc agaaccacgc cgtcgggttc tcgaattact tcgggctgca
    11521 ggcgcgcctg ctcaaaaata gcaaaaagtg ttgaaggggt gcttagagca ccttcttccc
    11581 ggcatctttt cccggcgtgc actcaaagac atcggtgatc ttgatgacga tgagcgattt
    11641 tgccgggtac ttgtggccct tcgccttcac ctggtcgcgg aacttctcgt aatcgggacc
    11701 ggacgtcttg atctccgtcg agccttttat ctggaaacag cgcttggtat cctggtcgta
    11761 gaagtagaga gccacgacgg gattctcttt gaggttctcc agtgtcttga ccatgtagtt
    11821 gtccatgatc cagacggtgt cgtcgttgac cagcagtatg gatgccatcg gtgccacgtt
    11881 cggtatgcct gccttcgacg cggtggccat cggcatgact ttggtctttg taaatatctc
    11941 tttaatctca cttgaaagcg caaccatatg gttcacctcg gggaacggaa cgggcgcggc
    12001 aaggcgccgg atcctttccg gtaatgtggt tatccgcgtt atcggtatat aaatggctct
    12061 gccggcccgg gcaggatggg tgcacaaccg cattctccac ccccttcgcg acctttgtgg
    12121 cagcgcgtga ggtggcagga gcgggcaacg gctggaccgc ttacgccggc gcgagggttc
    12181 gcgaaggggc ggggcggccg gcacccccgg gcgccacctt tctcccgcgt gatacgcgac
    12241 cggccggggg tgtggagcac cgccgccggc atgctgtatg cgtgccgaag tctccggccc
    12301 cgcccgaatc cgcacatatc cccggcaggg ccggcatacg gccgtccggc gtgccgggca
    12361 gaatctttgc cggggcatcc cgctccctcg cgttccgggc atatggacgg tatagaaaat
    12421 tataagcagg catagacgga aaagactact atgtgtgccc gctttttgga gcgctttttc
    12481 aaacctacgc cacatatcgt cgaaagcccg ccaccaccgt cgatctccca tggaccgggc
    12541 gagggtgttc ccgagtaccg ggtgaaaccc tattttatcg tggcgtctgt tgagatgggt
    12601 aataccacga caaagtgtat cctgaccggc acgaacctgg agacgggccg atcttacgtc
    12661 atcaacaaga ccgtgaccat gagccgggat gtccggcagc cgcgacccgg tgagacgatc
    12721 ttcggggaga ccctggacgg cacggagctg acccgggagt cggtgacgga cctggtgcgc
    12781 gataccctga tccagtgtca caacgaggct cacctgagca taaaggacga tctcgacttc
    12841 gtcgtcagga gcaccggcgt cgtggcggcg atggattctc ccgaccaggt cggggacttc
    12901 gtcatcgctc ttgcaaacgg ttgccttgac gcgggtgttc ccccgcggaa gatgacgcct
    12961 ccgatgtcga tcgacaacct tccgccgaaa ctccggcagt tctcgtttgc cgaccgggtg
    13021 gtcttcgtcg gcgcggtggc cggcgtcgtg ccgccggtcg ggtgtaccgg cgtcgagatg
    13081 gtggcaaacg agatggaggg ggagcttgcg atggcgggga taaaggaggg cgccaagtgg
    13141 acggcggtcg acttccggaa cccctgcata tccatcgact tcgggacgac gcttgacggg
    13201 cggatcacga gcgacgtcgc gcgcgacgac ccgaacccgt tcgcccagac ggtcgggaac
    13261 ttctgcgggc ttgccggggc cataccggac gcgatcgtcc ggggcaccgg gctcgtgaag
    13321 gggcggacgg ggacggccct cgatctcttt ggcgatcaca gcatcaaggg catgttcggc
    13381 ggccggaaac gttcggtcgt cgaggagtac gtcgaccggt gccacgagca tatcgatatc
    13441 cggatcgtcc cccccgaccg gacgaggttc gggcgggtgc cggtctgcgc ggaggtggcc
    13501 gacaagtcgg gcgttgccct catcgggtgc gatacgggtg tcaacgggag cgagacgccg
    13561 gctcttgagg cgatcgggca cgagatctac gaaaagcacg gcatgggcat gctgaccgag
    13621 gtcgtcgacc gggtctgcgc gaagatggcg ctccggctca tcgatgtcgc catcgcaagg
    13681 gggcacgtcc cgccgaactc gtcgataggg ttcaccgggc gggcggcgat atcggggcga
    13741 aaaccggagt acatcctcga gggaattacc gaacggaacc tctacgatac tccaaacgac
    13801 cacctggtct tcgtggacga cggtctggcc cggggtgcgg cgctgatggg gcggtgcatg
    13861 aactccctcg gaaagccgaa ggctccgctc ggcggcgtgc ggggagggcc atgtattatg
    13921 tcccgccgga tcaaaatagg aaagtaggat atgatacatg atggctgaag aagaactcta
    13981 cgatctgctc gttcccccgg gcgtaccccg gaagatgctg tacgacgttg ccgagaagta
    14041 cgacgtggaa gtggtgcg
//
LOCUS       DAOSII010000046        13747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_4555_length_13747_cov_48.165644, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000046 DAOSII010000000
VERSION     DAOSII010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 13747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13747
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4555_length_13747_cov_48.165644"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..938)
                     /locus_tag="PLV90_05010"
     CDS             complement(117..938)
                     /locus_tag="PLV90_05010"
                     /inference="COORDINATES: protein motif:HMM:NF019949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1724 domain-containing protein"
                     /protein_id="HOF96508.1"
                     /translation="MSALEVYGEFHDDIQVIFRSRLVTQVLLALGSEKRSLSTLRDIT
                     GSSSQALIPKIRQLEALQYVEQVRDGYTLTPIGRLVEQEIERLVTMMGVIERHKDFWA
                     AHDIESIPRRFLNELHDLYDAGVVRDIEEDVFAVYRAFGTIIESASWIHGVSSIMSPV
                     HAGAIREAALQGKPVELVVSQELAGKLVEEPYRTMLESLQGHANFGLYVSASPVRLGM
                     AVTDGFVSLGLYRRDKGTYDTATDLVSTDAAAIAWGERVFQHYRATADRLHLPSM"
     gene            1078..2379
                     /locus_tag="PLV90_05015"
     CDS             1078..2379
                     /locus_tag="PLV90_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOF96509.1"
                     /translation="MSFIPRYLICTALCACILIAGCSVPGGREPDIISGTTGDLAVHF
                     IDVGQGDSVLLQFRDKTMLIDAGERGMGGRVVTYLENLGVERLDVVVATHAHSDHIGG
                     LATVISAFPVGRFVDAGQAHPTATYENLLVLVEEKGIPYTAAVRGQTIALDPDLDIRV
                     LNPAAQPLGDVNEDSVVLKATYGDISYLFTGDAGKPAEESMAKAGLDLDADVLKVGHH
                     ASRYASSAEFLSAVSPAVSVILVGEGNDYGHPHEEALERLEAAGSRIYRTDRDGNVVV
                     TTDGQKIAVTAGGAPAATVTVTQTTSAITPAATTSTVTPAATTPSTTSAVTPAVTAPP
                     TGVTITYLSLLDEMVTVTNAGATAVNLTGWTIADEGARNTYTFPVFVLDAGSNVTVHS
                     GAGNETASDLYWGRSSPVWNNDGDTATLADASGAIVSVLEG"
     gene            2384..2617
                     /locus_tag="PLV90_05020"
     CDS             2384..2617
                     /locus_tag="PLV90_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HOF96510.1"
                     /translation="MQDESTFRASLDRVEEGLAVLLIREDESIRFTLPRSLLPADARE
                     GDILDVTVRRDTAATKEARRRVAERIERLRSRG"
     gene            2663..3751
                     /locus_tag="PLV90_05025"
     CDS             2663..3751
                     /locus_tag="PLV90_05025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOF96511.1"
                     /translation="MQDRCDVLVVGAGPAGSTAARYAAEAELDVLLIDKRREVGLPVC
                     CGEFNASSEVLRTALPAASVPDEPDLPRRAIRGAVAVSPGGREYGFDLPGYTLDRDAL
                     DRSLADAAVRAGAALSLETTFRGRDGRRIITDRGTITAGVVVAADGPLSLVSRSAGME
                     RCAVLAPAVTCRVDGEFGDRIRLYFGNSVAPGGYAWVFPKEGCANVGLGVQGRTTSVT
                     LLLRAFLARMGFIGRDVRAGFVPVAGPIPETVRENVIAVGDAAGQVVASNGGGIATAM
                     ICGRLAGIAAARHLLHDEPLRAYEHAWRSAVGRELGTAKRTKRMADWVLGSDFLIERA
                     MAALGDRGLRNVIVYGGLTAPRRRPERL"
     gene            3797..4054
                     /locus_tag="PLV90_05030"
     CDS             3797..4054
                     /locus_tag="PLV90_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOF96512.1"
                     /translation="MVKADGRKEPFAREKVVVSALKAGAPLEEARAIGEAVERDAYDG
                     MPAADIRQRVLSHLRDRNPEWEENWLMYDRAVKRREVLAAR"
     gene            4314..5729
                     /locus_tag="PLV90_05035"
     CDS             4314..5729
                     /locus_tag="PLV90_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOF96513.1"
                     /translation="MPTSVPKDDTHSHNNQNETEGTKLLLADPKTAILRLSLPVMVAM
                     TLMTLYSVVDAFWVAGLGADALAAVGFSFPLFIITIGLASGLGTGGEAALARMIGAGD
                     RDGASSVAMHTILLMTGLAVAVTIPLALFAGDIFALMGAGSAAGLAVAYARILFMGTF
                     ALLFGEVAYALMHGEGDAKRAMYAMAAGAVANIVLDPILIYTCGMGIEGAAVATIIAE
                     IVAAVPMVYWLFVKRDTYISLSLRRFAPDPAVARDILQVGLPAAAEQLSLALMTLVLN
                     GIIVLISSTDGVAVYSTGWRVVSIGLTPILAIATSVVAVTGASYGARAYARMESALRF
                     AIRLGTGIGIGLAAATFILAPGIAALFATPEITPELTSYLRVMSLVYPMVGFGLFSAS
                     FFQGTGLGARSLTITVLQNVVLSTLLAWVFAIDLGFGLSGVWWGVAAGNAIGALLGYA
                     WARRYTAGLRVRQAGAAVAAA"
     gene            complement(5746..7674)
                     /locus_tag="PLV90_05040"
     CDS             complement(5746..7674)
                     /locus_tag="PLV90_05040"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOF96514.1"
                     /translation="MGRVNPVFVRPEETFEILDEPGVGILVLDRDLRVAWANARAMDI
                     LPIGNDIPGADARRVLGALMQEEESVRKLLDGASDISGIDLPARGPRGEERRIRYSSR
                     KIEQGPFAGIRVLRLRDVAGEERYRSFFRDFQGIAYRTTESGPVLFHGAVEAITGYTE
                     DDFLEGAVCWDAVVHPDDRPGFQECAGELRAVPGLSTERVYRIVRKDGTTRWVRDFAK
                     NVCNEDGTPALVQGTIHDAIGRREADDGLLSSNRQLSVLNKIMGVAASSLSLDELLEA
                     SLSVTIDLLDLDVGLTYLLNPERTLALTRYHHAAPKRYLARSRTIKVHHWPWNFVFVA
                     GQPRYIERGGNPGAAEEEVLASLEVSALACIPILAESVVVGALFLGRRDRPRIRDEER
                     RLLEAIGREIGSGVLRSMLHKRLEAAHRETNLYLDVMTHDLKNAGNVANLYCDLLIDE
                     PGGAAAEYAKRLRESIRKSTGILQNVSTIRRIHQEPPDLRPVSLDRVIRAERERMPEA
                     AVTLEGTPAEVWADDLLSEIFANLIGNAVTFGGPGAGITIRVEGCAKENRMVVVTVED
                     TGPGIPDGVKEALLQRLGGDEDQVHGEGLGLSVVGMLVERYGGRVWIEDRVSGRPDLG
                     AAFRFTLREVARTERKPM"
     gene            complement(8023..8640)
                     /locus_tag="PLV90_05045"
     CDS             complement(8023..8640)
                     /locus_tag="PLV90_05045"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOF96515.1"
                     /translation="MATPSLRKYELPPLPYAPDALEPYISREQLTLHHDKHHQAYVTG
                     ANADLEKLEKARQEGAEVDMKALLKELSFNVGGQVLHTLFWPNMAPAGSGGGGKPGGA
                     LADRIDREWGSFDRFKAEFTKAAASTEGSGWAALSHCSLTDRLMIMQIEKHNNNVYPM
                     FGILMVLDVWEHAYYVDYKNNRGQFIDAFWNVVNWDTVNKRLENL"
     gene            8987..10534
                     /locus_tag="PLV90_05050"
     CDS             8987..10534
                     /locus_tag="PLV90_05050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOF96516.1"
                     /translation="MKTSARISFLTATLVVLLVLVLSRDILLILAGAGWSPAARALSL
                     LLIVVTAFGVIQFTNYADHLLRSLYVYPEHPLAPRQETAPPAGRVAVFIPVYNEEPGV
                     VESCIRACTSIAYPDCTVYLLDDSTDERLRGSMEEIGRRYRIRYVHRDHRKGFKAGAI
                     NHALSLLDGDTQYLLVLDADQIVKPAILAGLIPVLEADPTLSFIQTPQFFRPDPDNLI
                     SATFSYQQHIYNKHVCRGLSINRTTMLTGSNCIFRVGHLLAIGGMDETSITEDIATAF
                     LFHTGGYRGVYIDAVYAEGMAPPDLAAYFTQQLRWAYGNTRLLGKVMRQFVAHPRSLA
                     PVQWLEFAVIASVYLFGGLSVVFFLLPVATLLFGIPILPIWLPVVFAVVFGTVIAIQV
                     ATSIRERHYSPRDLAFSQAIFNTLSLVYIRAIWYAVSGKKLPFVVTPKTAFVSGERAR
                     VRITPLLAVIAAILLSMLAGIVRLLTRGPDFGTIVPLFWAGFTLLVLSSFIVVWRKEG
                     KAGARGS"
     gene            complement(10802..12844)
                     /locus_tag="PLV90_05055"
     CDS             complement(10802..12844)
                     /locus_tag="PLV90_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOF96517.1"
                     /translation="MAKRMLSEFESYDLLKQYGVPVPDHVVVKTAAEAGKAAEKIGFP
                     VVMKIYSPQIVHKSDAGGVIVSIPSKSAAEEAFEKIVKSAKAYNPEAEIKGVIVEQQA
                     APGLELIIGGKTDPAFGKVLTFGMGGTLVELMRDVTLRILPITEETIREMIQEIHGYP
                     MIKGYRGTKPRDEEALVQIMWAVNCFFAENTHVVEFDINPIRLYESGACIVDARIFVD
                     DEAVEKVAKERPFVPIEYFTPRSIAVIGASSEPKKMGYAVMHNLLHFPGQLYPVNNKR
                     PEVQGLKAYASILDIPNPVDMAVITVPAKHVPSVIEECGQKGVSMAVIITAGFKETGE
                     GGKALEDRILAIAKGYGTRIVGPNCLGLIIPPKGIDTTYVHESPKPGNIAFISQSGAI
                     VNTVVDWSIKHDIGFSAVVSVGNQADLNFIDFLRFVERDPKTKGIILYIEEIQDGKTF
                     MKVAGEVARTKPIVAIKSGSSEKGQAAASSHTGSLSGSYDVYMEAFRNAGVIPVHTLT
                     GTFQVAEMLSLPKGYPRGKRAVVVTNAGGFAVLSSDYAERYGIDLISLPPAVLKELND
                     LLPDFWNKNNPIDLLGDANEKRFEQTFNVLARHQDCWDIAFVVGFPNLVIGSDQLANQ
                     IIKFSEKTENMIVATLLGGGSMDRGRKILKENGIPLFEELDFTFRVMGRILWQRFR"
BASE COUNT         2285 a         4554 c         4307 g         2601 t
ORIGIN      
        1 gaatattcat cggtctggtt aacacgggcg cgctgctccg gcagggcggc acccctccat
       61 ccggtcggtt gtgggtgatc cccggggcgc gggctccggc tggagtcaaa cgagtactac
      121 atcgagggca gatggagccg gtctgcggtt gcccggtaat gctggaacac ccgttctccc
      181 caggcgatgg ctgccgcatc ggtgctgacg aggtccgtgg cggtgtcgta ggttccctta
      241 tcgcgccgat agaggccgag cgagacgaag ccgtcggtca ctgccatccc gaggcgaacc
      301 ggcgaggcgg agacatagag cccgaagttg gcatgcccct gcagggactc gagcatggtc
      361 cggtacggct cttctaccag tttcccggca agctcctgcg agacgacgag ttcgacgggt
      421 ttgccctgca gagccgcctc tctgatcgca cccgcatgca ccgggctcat gatcgaggat
      481 acaccgtgaa tccacgacgc gctctcgatt atggtcccga acgccctgta cacggcaaag
      541 acgtcctcct cgatgtccct gaccaccccc gcatcgtaga ggtcatgcag ttcgttcagg
      601 aacctccggg gtatgctctc gatatcgtgt gcggcccaga agtccttgtg gcgctctatc
      661 acgcccatca tcgtcaccag gcgctcgatc tcctgctcga cgagcctgcc gataggggtg
      721 agggtatacc cgtcccgcac ctgctcgacg tactgcaggg cctcaagctg ccggatcttc
      781 gggatgaggg cctgcgacga actccccgtg atgtcgcgga gggtcgagag ggacctcttc
      841 tcgctcccga gggcaagcag cacctgcgtt accagcctcg accggaagat cacctgaata
      901 tcgtcgtgga actcgccgta gacttcaaga gcggacatac catcgcctta taattaagaa
      961 gcgccggccg gcgctatcac tcttttcatt cctgcacgcc ctctccctcc cccgacgtgc
     1021 cacccaatcc gaccccagcg cacttatagc cgggaacccg atacctctac aggcagcatg
     1081 tcgttcatac ccagatacct gatctgcacc gcactctgtg cctgcatcct catcgccggc
     1141 tgctccgttc ccggcgggcg agaacccgat atcatctccg ggaccaccgg ggaccttgcc
     1201 gtccacttca tcgacgtcgg gcagggcgac tcggtcctcc tccagttccg cgataagacc
     1261 atgctcatcg acgccggcga acgcggcatg ggggggcgcg tcgtcacgta ccttgagaat
     1321 ctcggcgtag aacgtctcga cgtggtggtg gcgacccatg cccactccga tcacatcgga
     1381 ggacttgcca cggtcatatc cgccttcccg gtcggccggt ttgtcgacgc cgggcaggcg
     1441 caccccaccg cgacgtacga gaacctgctc gtcctggtcg aggagaaggg gataccgtat
     1501 acggcggcag tgcgcgggca gacgatcgcc ctcgaccccg acctcgatat ccgggtcttg
     1561 aacccggccg cacagcccct cggggacgta aacgaggatt cggtcgtcct gaaggcgacg
     1621 tacggggata tatcctatct cttcacgggc gatgccggga aaccggcgga ggagagcatg
     1681 gcgaaggccg ggctcgacct cgatgccgac gtcctgaagg tcggccacca cgcgagccgg
     1741 tacgcatcct ccgccgagtt cctctccgcg gtcagccccg cggtcagcgt catactcgtc
     1801 ggcgaaggaa acgactacgg ccacccccac gaggaggctc tcgagcgcct cgaagccgcc
     1861 gggtcgcgga tctaccggac cgaccgcgat gggaacgtcg tcgtcacgac cgacggccag
     1921 aagatcgccg ttacggccgg cggagccccg gcggcgaccg tgacggtcac gcagacgacc
     1981 tcagcgataa ccccggcggc aacgacctca acagtgaccc cggcggcaac aaccccgtca
     2041 acgacctccg cagtaacccc ggccgtgact gctcccccca ccggcgtcac catcacctac
     2101 ctcagcctcc tggatgagat ggtcaccgtc acgaacgccg gcgcgaccgc cgtcaacctc
     2161 accggctgga cgatcgccga cgaaggagcg cgcaacacct acaccttccc ggtcttcgtc
     2221 ctcgacgcag gctcgaacgt caccgtccac tccggcgcgg gcaacgagac cgcgtccgac
     2281 ctctactggg gacgctcatc cccggtctgg aacaacgacg gcgataccgc cacgctcgct
     2341 gacgcgagcg gcgcaatcgt cagcgtcctg gagggatgag aggatgcagg acgagtcgac
     2401 cttccgtgca agcctcgacc gcgtggagga ggggcttgcc gtcctcctca tccgcgagga
     2461 cgaatcgatc cgcttcacgc tcccccgctc cctcctcccc gcggatgccc gggagggcga
     2521 catactcgac gtgacggtcc gccgggacac cgcggcgaca aaagaggcgc ggaggcgggt
     2581 ggcggagagg atagagcggc tgcggtcgcg gggatagagc accccactct tttatcccct
     2641 cccccgcctc acgtgagcgc cgatgcagga tcggtgcgac gtcctcgtcg tcggtgcggg
     2701 ccccgccggc agcaccgccg cacgatacgc ggcggaggca gaactcgacg tcctcctgat
     2761 cgataagcga agagaggtcg gcctgcccgt ctgctgcggg gagttcaacg cctcgtccga
     2821 ggtcctcaga accgcgctcc ccgccgcctc cgtgcccgac gaacccgatc tcccccggcg
     2881 ggcaatccgg ggcgccgtcg ccgtctcccc cggcggccgg gagtacgggt tcgaccttcc
     2941 cggctacacc ctcgaccggg atgcgctcga ccgcagcctc gcggatgccg ccgtccgtgc
     3001 cggcgccgcc ctctcgctgg agacgacatt tcgcggccgg gacggacgga ggatcatcac
     3061 cgaccggggc acaatcacgg ccggcgtcgt tgtcgctgca gacggcccgc tctcactggt
     3121 ctcccgctcc gccggtatgg agcggtgcgc cgtcctcgcc cccgccgtca cctgccgggt
     3181 cgacggggag ttcggcgacc gcatccgtct ctacttcggg aacagcgtcg ccccgggcgg
     3241 gtacgcgtgg gtcttcccga aggagggctg cgccaacgtc gggctcggcg tgcagggaag
     3301 aacgacatcc gtcaccctcc tcctgagagc cttcctcgcg cggatggggt ttatcggccg
     3361 cgacgtccgg gccgggttcg tccccgttgc cggccccata ccggagaccg tcagggagaa
     3421 cgtcatcgcg gtcggcgacg ccgccggcca ggtcgtcgcc tccaacggcg gggggattgc
     3481 gaccgcgatg atctgcggga ggcttgccgg catcgcggcg gcccgccacc tgctgcacga
     3541 cgaacccctc cgcgcctatg agcacgcctg gcgctcggct gtaggccggg aactcggtac
     3601 cgccaaaagg acgaagagga tggccgattg ggtcctcggc tccgacttcc tgatcgaacg
     3661 ggcgatggcg gcgctcgggg accgagggct gcgaaacgtc atcgtctacg gcggcctgac
     3721 ggccccccgg cggcgaccgg aaaggttata gcccctcacc gcccctcctc gcccggaggc
     3781 gatcgaactg gtcaacgtgg tcaaggccga cggccggaaa gagccgtttg cacgggaaaa
     3841 agtggtcgtg agcgccctga aggcgggcgc gccgttagaa gaagcgcggg cgatcgggga
     3901 ggccgtcgaa cgggacgcct acgacggtat gccggccgcc gatatccggc agagggtgct
     3961 ctcccacctc cgcgacagaa accccgaatg ggaggagaac tggctcatgt acgaccgtgc
     4021 cgtgaagaga cgcgaggtat tggcggcccg ctgatctcct cgcacttttt tgccctctcc
     4081 ccgtgggaaa gtataattac aatccgcgcc cacatctgac ctgtatcgtc ctatgcgccc
     4141 tggaaatcgc agaactggga aggccaacaa caggccgttt ctactcctgt ccaatatctg
     4201 aggagcgttc gatgatcagt gttcacagat aggtgcgatg cccgtaccgc acgcaaaggg
     4261 cgtcccgatt gaaccgggag ctcgcggcac gggcacgcca gcaggcgata tgtatgccga
     4321 caagcgtccc aaaagacgac acacactcac ataacaacca gaacgaaaca gaaggaacaa
     4381 agttactcct tgccgatccg aagaccgcga tcctccggct ctctctgccg gtgatggtcg
     4441 cgatgaccct catgaccctc tacagcgtcg tcgacgcgtt ctgggtcgcg ggtctcggtg
     4501 ccgatgctct tgctgcggtc gggttctcat tcccgctctt catcatcacc atcgggttag
     4561 cgagcggcct cgggaccggg ggtgaagcgg ctctcgcgcg catgatcggt gcaggcgacc
     4621 gcgacggcgc aagcagcgtt gccatgcaca ccatcctcct gatgaccggc cttgccgttg
     4681 ccgtcaccat cccgctcgcc ctctttgccg gggatatatt cgccctcatg ggtgcgggca
     4741 gcgccgccgg gcttgccgtc gcgtatgccc ggatcctctt tatggggacg tttgccctcc
     4801 tcttcggcga ggtcgcctac gcgctcatgc acggtgaggg cgacgcgaag cgggcgatgt
     4861 acgctatggc cgcaggggcg gtggcaaaca tcgtactcga cccgatcctg atctacacgt
     4921 gcggcatggg gatcgaggga gccgccgtcg cgaccatcat cgccgagatc gtcgccgccg
     4981 tcccgatggt ctactggctc ttcgtgaaga gggacaccta catatccctc tcactccggc
     5041 ggttcgcccc cgaccccgcc gtcgcgcgcg atatcctgca ggtcggcctt ccggccgccg
     5101 ccgaacagct ctcgctcgcc ctgatgaccc tggtcttaaa cggcatcatc gtcctcatat
     5161 cgagcaccga cggcgtcgcc gtctactcga ccgggtggcg cgtcgtgagc atcggcttaa
     5221 ccccgatact tgccatagca acgtcggttg tcgcggtcac cggcgcctcc tacggggcgc
     5281 gggcgtatgc caggatggag tctgccctcc gcttcgccat caggctcggg accggcatcg
     5341 gcatcgggct tgccgcggcc acgttcatac tggcccccgg gatcgccgcg ctcttcgcga
     5401 caccggagat cacgccggaa ctgacgtcgt atctccgggt catgagcctc gtctacccga
     5461 tggtcgggtt cggcctcttc tcggcgtcgt tcttccaggg gacggggctt ggggcacggt
     5521 ccctgaccat cacggtcctg cagaacgtcg tcctctcgac cctgcttgcc tgggtgttcg
     5581 caatagacct cgggttcggc ctctccggcg tctggtgggg cgtcgccgcc ggaaacgcca
     5641 tcggcgcatt gctcggttac gcctgggcac gccgctacac ggcggggctt cgggtccggc
     5701 aggccggagc ggcggtggca gcggcgtgag cgggggttct ccccctcaca tcggtttacg
     5761 ctccgtgcgg gcaacctcgc ggagcgtgaa ccggaacgcc gccccgaggt ccggcctgcc
     5821 gctgacccgg tcctcgatcc agacccggcc gccgtagcgc tcgaccagca tgccgacgac
     5881 ggagaggccg agcccttcgc cgtgcacctg gtcttcgtcc ccgccgagcc gctggaggag
     5941 cgcctctttc acgccgtcgg gtatgccggg tccggtgtcc tccacggtca ccaccaccat
     6001 gcggttctct tttgcgcacc cttccacccg gatcgttatg ccggcgcccg gcccgccgaa
     6061 cgtgacggcg tttccgatga ggtttgcaaa tatctccgag agcaggtcgt ccgcccagac
     6121 ctccgccggc gtcccctcga gcgtgacggc cgcctccggc atacgctctc tctccgcgcg
     6181 gatgacgcgg tcaaggctga ccggcctgag gtcgggcggc tcctggtgaa tccggcggat
     6241 cgtcgagacg ttctggagga tgccggtgct cttccggata ctttcccgga gtctctttgc
     6301 gtattctgcg gcggctcccc ccggttcgtc gatgaggagg tcgcagtaga ggtttgcgac
     6361 gttgccggcg ttcttgaggt cgtgggtcat gacgtccagg tagaggttcg tctcgcggtg
     6421 ggccgcctcc agccgtttgt ggagcatgct ccggaggacg cccgagccga tctctctccc
     6481 gatcgcctcg aggaggcggc gttcttcgtc gcggatccgc ggccggtccc gcctcccgag
     6541 gaagagcgca ccgaccacga ccgactccgc gaggatcggt atgcaggcga gggcggagac
     6601 ctcgagcgat gcgaggacct cctcctcggc cgcgcccggg ttgccgcccc gctcgatgta
     6661 gcgcggttgt ccggcgacaa agacgaagtt ccagggccag tggtggacct tgatcgtccg
     6721 gctgcgggcg aggtagcgct tcggggcggc gtggtggtag cgcgtgagcg ccagtgtccg
     6781 ttccgggttg agcaggtagg tcagccccac gtcgaggtcg agcaggtcga tcgtcaccga
     6841 gagcgacgcc tcgagcagtt catccagcga gagcgacgag gcggcgacgc ccatgatctt
     6901 gttcaggacc gagagctgcc ggtttgacga gagcaaccca tcgtcagcct ctcttcgccc
     6961 gatggcgtcg tggatggttc cctggacgag ggcgggcgtg ccgtcctcgt tgcacacgtt
     7021 cttcgcgaag tcgcggaccc accgggtcgt gccgtccttt cgcacgatcc ggtagacccg
     7081 ctcggtcgag aggcccggga ccgcccggag ttcgccggcg cactcctgaa aaccggggcg
     7141 gtcgtcgggg tgcacgaccg catcccagca tacggctccc tcgaggaaat cgtcttcggt
     7201 atacccggtg atcgcctcca ccgcgccgtg gaagaggacg ggcccggact ccgtcgtccg
     7261 gtaggcgata ccctggaagt cgcggaagaa cgagcggtac cgctcctcgc cggcgacgtc
     7321 ccgcagccgg agcacccgta tccccgcaaa cggcccctgc tctatcttcc ggctggagta
     7381 gcggatccgc cgttcttctc cccgcggacc ccgggccggg agatcgatgc cggatatatc
     7441 gctcgcgccg tcgagaagtt tccggacgct ctcttcttcc tgcatgaggg cgccgaggac
     7501 ccggcgggca tcggcgccgg gtatgtcgtt gccgatcggg aggatatcca tggcccgggc
     7561 gttcgcccag gccacccgca gatcgcggtc gagcacgagg atacccaccc cgggctcgtc
     7621 gaggatctca aacgtctctt ccggtcggac gaataccgga tttactctgc ccatatggtt
     7681 tgttcccccc tcagagattt ttatatagat gagtttatgt aattggatcg ggtcccgggc
     7741 gataagagat ttgcggttgc ggcccgcctg atgccgataa ccctgcagcc tcacgcgcga
     7801 ggtgagaggc tgtgcgtggg gtcaaacctg cgtgcaggca tccctgcagc ctcacgcgca
     7861 aggtgagagg gcatgagcgg tgaaacggtc tctcgcgttg cctcggtggg ggttgcaggg
     7921 accggggaag ggccttcgcg gcttcgcgcg agttcaggta ggggcggtga aacggcgttt
     7981 caacagagga ctgacggggc gcttttccgc cgaaaagagg ggttagaggt tttccagtct
     8041 cttgttcacc gtatcccagt tcacgacgtt ccagaacgcg tctatgaact ggccgcggtt
     8101 gttcttgtag tcgacgtagt aggcgtgctc ccagacgtcg agcaccatca ggataccgaa
     8161 catcgggtag acgttgttgt tgtgcttctc gatctgcatg atcatgaggc gatccgtcag
     8221 gctgcagtgc gagagagccg cccagccgga cccctcggtg ctcgcggcag ccttcgtgaa
     8281 ctccgccttg aaccggtcaa aggagcccca ctcccggtct atccggtctg caagggcgcc
     8341 gcccggtttg ccgccgccgc cagagcctgc cggcgccatg ttcggccaga agagggtgtg
     8401 caggacctgg ccgcccacgt tgaacgagag ctcctttaag agcgccttca tgtcgacctc
     8461 ggccccttcc tgccgcgcct tctccagttt ctcaaggtcg gcgttcgccc cggtcacgta
     8521 cgcctggtgg tgcttgtcat ggtggagggt gagctgctct cttgatatgt acggctcgag
     8581 agcgtcaggt gcgtagggca ggggcggtag ttcatacttc cttaacgagg gagttgccat
     8641 catatgtatc atttgatgga tggggaacaa cctatttcag agtgtcggtt gccgtgcggc
     8701 cgtgcggccc ctctcttcct tgccccggaa ggccccggat gtgtcgcgga tatcaataaa
     8761 agcccgtact ctcgaacgtt gtgcggcacc ggcacggccg ctccctgcgc cggcgaataa
     8821 ctccccgggg atgagtcttc atggtcccgg ggcggccggg accataaggg ttaaataatc
     8881 cggtacagtg ggtggtaact tacattgcct cgcgcaccct ccgttgcaga aagcccggtg
     8941 ccgtgtgccc gcgaggcatc ccgcacgcga gagtggtgga taccccatga agacgtcagc
     9001 gcgtatatcg ttcctcaccg ccacgctcgt ggtgctgctc gtgctcgtcc tctcccgcga
     9061 tatcctcctg atactcgccg gagccggatg gagccccgct gcgagggcgc tctctctcct
     9121 cctcatcgtc gtcacggcgt tcggggttat ccagttcacg aactacgccg atcaccttct
     9181 acggtcgctc tacgtctacc ccgaacaccc ccttgcgccg cggcaggaga ccgcaccgcc
     9241 cgccggccgc gtcgcggtct tcataccggt ctacaacgag gagccgggcg tggtcgagtc
     9301 ctgcatccgg gcgtgcacgt cgatcgccta tccggactgc acggtctatc tcctcgacga
     9361 ctcgaccgac gagcgcctcc gcggttcgat ggaggagatc ggccgccgtt atcgtatccg
     9421 ttacgtccac cgcgatcacc ggaaggggtt caaggcgggg gcgatcaacc acgccctctc
     9481 cctccttgac ggcgatacgc agtacctcct ggtgctcgat gccgaccaga tagtgaagcc
     9541 ggcaatcctt gccggcctca taccggttct cgaggccgac cccaccctct cgttcatcca
     9601 gacaccccag ttcttccggc cggacccgga caacctcata agcgccacgt tctcctacca
     9661 gcagcacatc tataacaaac atgtctgcag ggggctctcc atcaaccgga ccaccatgct
     9721 gaccggctca aactgcatct ttcgcgtcgg ccaccttctc gccatcgggg ggatggacga
     9781 gacctccatc accgaggaca tcgccaccgc ctttctcttc cacaccggag gctaccgggg
     9841 ggtctacatc gacgccgtgt atgccgaagg catggcgccc cccgacctcg cggcgtactt
     9901 cacgcagcag ctccgctggg cctacgggaa cacccggctc ctcgggaagg tgatgcggca
     9961 gttcgtggcg cacccgagaa gcctggcccc ggtccagtgg ctggagttcg ccgtgatcgc
    10021 ctcggtctac ctcttcggcg gtttgagcgt ggtgttcttc ctcctcccgg tcgccacgct
    10081 cctctttggc atccccatcc tgccgatatg gcttccggtg gtctttgccg tggtctttgg
    10141 cacggtgatc gccatccagg tcgccaccag catacgcgaa cggcactact ccccgcgcga
    10201 cctcgcgttc tcgcaggcga tcttcaacac gctctctctc gtctacatcc gggcgatctg
    10261 gtatgcggtc tcggggaaga aactgccgtt cgtggtgacg ccaaagacag ccttcgtatc
    10321 gggggagagg gcccgtgtcc ggataacgcc cctccttgcc gtgatcgcgg ccatactgct
    10381 atcgatgctc gccggcatcg tgcggcttct cacccggggg ccggacttcg gcacgatcgt
    10441 tccgctcttc tgggcaggct tcacgctcct cgtcctctcg tcgttcatcg tggtctggcg
    10501 gaaggaagga aaggcaggcg cccggggatc gtgatgcgtg aggggtgccg gcagaggcgg
    10561 tcgccgctat gggcactacg gcatctcgcg aggccgtatg gttatccgca ccgggcaacc
    10621 tcccgcggaa gtttgcgaag ttcggcggat ggccccctgg aactcctcgt caaggctccg
    10681 cgcgaagccg tttcaccgcc cgtatacctc gcgcgaagac ccgttccggg taccgttgcg
    10741 gatctcgcct gctatggagg tagcaccaat agaggcgaca aaaaatattc ggagaggaga
    10801 tttaccggaa tctctgccag agaatccgtc ccatgacccg gaaggtgaag tcgagttcct
    10861 cgaagagcgg gatgccgttc tctttgagga tctttcggcc cctgtccatg gacccgccgc
    10921 caaggagcgt cgcgacgatc atgttctcgg tcttctccga gaactttatg atctggtttg
    10981 cgagctgatc cgacccgatg acgaggtttg ggaacccgac gacgaacgcg atgtcccagc
    11041 agtcctggtg tctggcaagc acgttgaacg tctgttcaaa ccgcttctcg ttcgcatcgc
    11101 cgaggaggtc gatcgggttg ttcttgttcc agaagtcggg gaggagatcg ttgagttcct
    11161 tgagaaccgc cggcggcagg gagatcaggt cgatgccgta gcgctcggcg tagtcggagg
    11221 agagaacggc aaacccgccg gcgttcgtga cgacgaccgc acgcttgccg cgggggtagc
    11281 ccttcgggag cgagagcatc tccgcgacct ggaacgtgcc ggtcagggtg tggaccggga
    11341 tgacgccggc gttgcggaag gcctccatgt agacgtcgta cgaccccgag agcgaacccg
    11401 tgtgggacga tgccgccgcc tggcctttct cggacgaacc ggacttgatc gccacgatgg
    11461 gcttggtcct ggcgacctcg cctgccacct tcatgaacgt cttgccgtcc tggatctcct
    11521 cgatgtagag gatgatgccc ttggtctttg ggtcgcgctc cacgaaccgg aggaagtcga
    11581 tgaagttgag gtccgcctgg ttgccgaccg agacgacggc ggagaacccg atatcgtgct
    11641 tgatgctcca gtcgacgacg gtgttgacga tggcgccgct ctgggagatg aaggcgatgt
    11701 tgccgggttt cggggattcg tggacgtagg tggtgtcgat gcccttcggg gggatgatca
    11761 gaccgagaca gttcgggccc acgatccgcg tgccgtaacc cttcgcgatc gcaaggatcc
    11821 ggtcctcaag cgccttgccg ccttcgcccg tctctttgaa cccggccgtg atgatgaccg
    11881 ccatggatac gcccttctgc ccgcactcct cgataacgga gggcacgtgc tttgcaggga
    11941 cggtgatgac cgccatgtcg accgggtttg gtatgtcgag gatggaggca tacgccttga
    12001 gcccctgcac ctccggccgc ttgttgttca cggggtagag ctggcccggg aagtgcagga
    12061 ggttgtgcat cacggcgtac cccatcttct tgggctcgga cgacgccccg atgactgcga
    12121 tggagcgggg cgtgaagtac tcgatcggca cgaacggccg ctcttttgcc accttctcga
    12181 cggcttcgtc gtcgacgaat atgcgggcgt cgacgatgca ggcccccgac tcgtacagcc
    12241 ggatcgggtt gatgtcgaac tccacgacgt gggtgttctc ggcaaagaag cagttgaccg
    12301 cccacatgat ctggacgagc gcctcctcgt cccggggttt cgtcccgcga tagcccttga
    12361 tcatcgggta gccgtggatc tcctggatca tctcccggat cgtctcttcc gttatgggga
    12421 ggatccgcag ggtgacgtcc ctcatcagct cgacgagcgt gccgcccatg ccgaaggtga
    12481 gcaccttccc gaacgccgga tcggtctttc cgcctatgat cagttcaagc cccggtgccg
    12541 cctgctgctc gacgatgacg cccttgatct cggcttcggg gttgtaggct tttgcactct
    12601 tgacgatctt ctcaaacgcc tcttcggcgg ccgattttga ggggatactg acgatcacgc
    12661 cgcccgcatc gctcttgtgg acgatctggg gcgagtagat cttcataacg acgggaaaac
    12721 ctatcttctc cgcagccttg cccgcctcgg cggcagtctt gacgaccacg tggtcaggta
    12781 ccggtacgcc gtattgtttc aggagatcgt acgattcgaa ttcgctcaac attcttttgg
    12841 ccatggttat ctcctcacat cttgagtctc gtatcgagca tcagtatgat tctacagttc
    12901 gtgttcagat tgcccgtctc ccggaaagtc aggccggaag gccggaatga actgtacagg
    12961 gtggtctcat atccgtttta taggttttgt aatgatttta catgacagac cggcgaagga
    13021 gcgccgccgg gccccagaat actgtctttc cgcattcaac cgggcaatac tccggagtaa
    13081 cggcgttttc cggtgagcct cgcgggaggt cccctctccg gatggcctgc tcccggggcc
    13141 ggggtcttcg ccgaaattcg ccggcgggcg gtgccgtggt gagactcccg gtccatcgat
    13201 ccccgcgtct cctcccggcg ctggcgaccg ccggagaggc aaaacacacc gggaggaacc
    13261 gcggtcgggt gcctcccgga tctggtgtgt accagaaacc tcgtgttaga tatattcatc
    13321 aatattataa aaatggtatt atttttcatg gtgcctgcag gagcctcctt tccggaaagg
    13381 gggtaccggt cagcccggaa gaggggccgg gatctgcccg gggcgggctc gcggcgtacc
    13441 ctggggcttt ttgaagggca ggcgcctctc ccgcgatccc ccgggatggc gggagcgccc
    13501 ccctcctccg ggaacatttc ggagatggga tataccggga ccggtcactc tcaaccggag
    13561 aaccatctct cgtgctcacc aggccgggag ggaggagaga ccatgaacga gcgtcaccag
    13621 atgccggata gccgggccgc gtatatcctc tttgcgatcg gcttttttgc catattctcg
    13681 accacgatag caaagaaccc ggtgctgccc ctcttctcgc aggccctggg cgccggggac
    13741 gcggtcg
//
LOCUS       DAOSII010000047        13661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_4598_length_13661_cov_44.518374, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000047 DAOSII010000000
VERSION     DAOSII010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 13661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13661
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4598_length_13661_cov_44.518374"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..593
                     /locus_tag="PLV90_05060"
     CDS             105..593
                     /locus_tag="PLV90_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96518.1"
                     /translation="MSVTKYSAQVVERILVAALLASLLLVSGASALPCTAAGGIAVAP
                     ADDLSDRLELLRSVGGTIHPGEHQTIWKSVPSDVRTLQVYLDWSGHQHPGTDSLTLTI
                     TPPGNGVLGPYRDGADGRTDEKIALTLSGSDPLPSGMWKFLIFGEDVPEEGTEYTLNV
                     GY"
     gene            615..1433
                     /locus_tag="PLV90_05065"
     CDS             615..1433
                     /locus_tag="PLV90_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOF96519.1"
                     /translation="MRIELCGRILSFFFLALALFALLWSPAAAAKIIVEPGPAELPTD
                     VTYIDDEWFVPVWQAYSPLEFLTIVLLIHCPLLAMPVELVYSAGVMAYLGYRTSRSPL
                     DNTNRHRIYACIRDHPGIAPAEIARATGINRGTVHYHLSCLRDAGLVGILRRKGRGGY
                     FRNGDCNPTEKAMYLHLNNDIRRQILALLLEKPGITQSEIADIMGIARSTAAWHLQKL
                     AADELVESDRDGRTVHYALTCEALAFSGALSSWGTLFLPGDKTSAGNCADSLPQ"
     gene            1816..2598
                     /locus_tag="PLV90_05070"
     CDS             1816..2598
                     /locus_tag="PLV90_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOF96520.1"
                     /translation="MARWSRILLVLIIAMILALPGTATARYVVEPVAEEYAGEWHDLE
                     EITFRDLPLRVMLLHLLFTILPGLPYIGELFYALSLLVPLGFRRVMRHNILDDDLRRD
                     LYRQITANPGIGTTELGELTGASRGRLRYHLDMLIREGKVAAVSYRNRARHFAGNQRY
                     TALEERIFASLRDETPAVILTHLHRSPGATQNEIKDQLGLAAPTISQHMQQFEAEGIV
                     VRDQEGRSFRYRLTGDAREILNEHQKFPTLKSASLRKWRSVS"
     gene            complement(2968..3597)
                     /locus_tag="PLV90_05075"
     CDS             complement(2968..3597)
                     /locus_tag="PLV90_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HOF96521.1"
                     /translation="MKIVVSVEEPGIIDQVLEYNPTFIEVRLDRMEGDLPDLVRAIRE
                     KTAIPLIATLRSREEGGRFVGDADLWMRVVGPVTRYVDYVDVEARYRDHAPFIKSQGV
                     QIVASLHTNEMPTPPELGRIEALLRSYGDIPKIVVKPRTADDLLTLVTFTHQAQKPIC
                     TSIMGAGFRYARAVLPLFGSEFVFCHAGTPTAEGQYHIREMRQLADLLK"
     gene            complement(3600..4430)
                     /locus_tag="PLV90_05080"
     CDS             complement(3600..4430)
                     /locus_tag="PLV90_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOF96522.1"
                     /translation="MNSTGIIGYGHMGSMLVKGFITSGALRADEIVVASRSRRSLDAC
                     AAAWPGVGIAATNADIARRCRTVIIAVRPQEVGGVLCEIAPALAGDEHIVSLAAGISL
                     AAIEDAYTGPVTRVVPTITSAVGEGTTLVCHGRQVGMDNALRVERLFSAIGSVVAVSE
                     ADLPAATLLSSCGPGLLASLIEELAGAAVRASGIPPDRALLLATEMVAATGAYLRETG
                     RAPGDLVGEVATGGGITEVGVRGFRERLPAVFDEVFAAMLERYGTVGRPLSDERSYPR
                     "
     gene            4484..7063
                     /locus_tag="PLV90_05085"
     CDS             4484..7063
                     /locus_tag="PLV90_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOF96523.1"
                     /translation="MEYIGRHGERYPTMPHDHPQGTGPVKPPELLAPAGSPEALAAAV
                     AAGADAVYLAGRRFGARHYAANFSDEELRSAIDYAHLHDVRVFVTVNTLVRDAELSDV
                     ARYLLWLYEAGADAVLVQDAGVASLAREIVPDLPLHASTQMTVHNREGVARAAREGFS
                     RVVLAREVTLAEIEDIAEVQGIGIEVFAHGALCYCYSGQCLLSSVIGGRSGNRGMCAQ
                     PCRKPYRLVTADKDDYGRPKSLRAVPAEDRYLLSTRDLAVYPHLDRIARASVASLKIE
                     GRMRSPEYVATVVDIYRRALDTIAAGAPWSPSREDMRDLALAFNREFTEGYILGAPDI
                     MARDRPGNRGVLLGTVSGYDPRRREASVRLAGDTSPRSGDGLAFSSDDPDRDVGAIVR
                     GTPAVKNGMVRIGVGAPVGAGARVFLTKSADLEERAKRIMANPLPQIPIDILVTWEGG
                     RPSLEATVPVCGGEPLRVLYRADIVMEPARSRPLTGEQIAEHLGKTGGTPFTVRKMDL
                     RYPGGLFTPIGELNRIRRTFLERVGEAVLAARRPGPDAVHAARERVEAAIEGMTRTAG
                     SRRTPRTPSVSVYVDTLEAAAAAAREGARTIYIEPSGPVGLRFLEEAATACREGGADL
                     VWKWPTVTRRGFLDAAAPLLPSLYDAGIRGVMVSGMGAADAVRNAEPRMRLYGAAGLN
                     VWNHRTAGSLSALFLRCTVSPELPAADVAELAGSVGNTPELEVIVQGTIEAMVTEDRL
                     VAAVAGEEPGNRFLGLDDQRRRIFPLRHDSEGRTYIANAAEICLIDRLPGVAGMGIDA
                     VAIDARGRGACYAGDMVRLYREGIDAVNRGDTGILPALKDEVRRRALGGITGGHFVRG
                     LAE"
     gene            7151..7819
                     /gene="tpiA"
                     /locus_tag="PLV90_05090"
     CDS             7151..7819
                     /gene="tpiA"
                     /locus_tag="PLV90_05090"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOF96524.1"
                     /translation="MVSPFILVNLKTYQEGMGSNAHRIAAAAEAVARESGVAIGIAPV
                     FTEIRPLSQHYAIPVYAQHIDAVTPGAHTGHILPEAVRAAGARGTLINHSERRLTLAD
                     IDACVENARRLHLESVVCTNNDATSAAAAALRPDYVAIEPPELIGSGISVSKADPGII
                     ERSVSAVRAVNPDVKVLTGAGIQSGECVKIAVDLGTCGVLLASSVVKAADPEAVFRDL
                     ISLL"
     gene            complement(7824..8378)
                     /locus_tag="PLV90_05095"
     CDS             complement(7824..8378)
                     /locus_tag="PLV90_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOF96525.1"
                     /translation="MEIPTPAELREKRLRMGLKQADVARMAGISQSMVARIEAGSVDP
                     RVSTLAKIVDVLQAAEHSTVTAATVMTAPVLSVTPDDPVNRAVEIMGQNGISQLPVLE
                     GGVPVGCISESAIMSAMEEGGLHQTHRKLVQDYMEPGFPTVPPTAPIDTIVHLLHHNH
                     AVIVVEQGKVQGVITKHDLISLIT"
     gene            8501..8764
                     /locus_tag="PLV90_05100"
     CDS             8501..8764
                     /locus_tag="PLV90_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96526.1"
                     /translation="MAVNREQQQKVLTKVREILSTYHTRDAVKIELESRGFTIRAEHG
                     DVVSLENTPAEIFVQLFVNDRGDIVDTHVVTFEEIELRPPAKE"
     gene            complement(8793..9133)
                     /gene="rnpB"
                     /locus_tag="PLV90_05105"
     ncRNA           complement(8793..9133)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLV90_05105"
                     /product="RNase P RNA component"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00373"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00373"
     gene            9297..9848
                     /locus_tag="PLV90_05110"
     CDS             9297..9848
                     /locus_tag="PLV90_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96527.1"
                     /translation="MGGSEASTQLELAAIFAAFVAVTTLFSFVSIGANALGGKIPEGA
                     GVYPVLAAGEPRLAPVGDIIGYPSVPGPSGTRIDALTFVIANTGGEGAVDLSRATVTV
                     MAGNYLEVLTRSEDLHPEPGAWTTSLPGDSDGNVLLRAGEECAVHIRLDHLIPAGETV
                     TVRVRLPGDRPCLIAGQAGHPED"
     assembly_gap    9892..9991
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10033..10599
                     /locus_tag="PLV90_05115"
     CDS             10033..10599
                     /locus_tag="PLV90_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00296 family protein"
                     /protein_id="HOF96528.1"
                     /translation="MEMLTPEEGRTVVRLARSAIEKTLSGERAVLPDLPAVFREKRGV
                     FVTIKQQGHLRGCIGLPYPIKPLGEAIVEAAISAALEDPRFPPVSRSEVAGIDLEVTV
                     LTEPRPLDCPPDERPGCVEVGRHGLIVSGHGTGGLLLPQVPAEYGWTSREFLDQTCIK
                     AGLPPGCWKRGSVAVLTFEGQIFDEKAV"
     gene            10605..12056
                     /gene="tgtA"
                     /locus_tag="PLV90_05120"
     CDS             10605..12056
                     /gene="tgtA"
                     /locus_tag="PLV90_05120"
                     /EC_number="2.4.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(15) transglycosylase TgtA"
                     /protein_id="HOF96529.1"
                     /translation="MTITFEVIHKDIAGRVGKLRVNDKAVRTPALLPVVNPHLPLVTP
                     REMQEMGVEALITNAYIFRRSTEFCDRALAEGLHAVLDFDGAVMTDSGSFQLSVYGEV
                     EVSNRETLEFQQAIGSDIIVPLDIPTPPDASRERAEQELAVTMDRIREAQALFPDANL
                     AGPVQGGIFTDLREEAGRAVRDLGVAFAPIGAVVPLMENYRYQDLVRVVLAAKRGLSP
                     ATAIHLFGAGHPSMFALAVAMGCDLFDSAAYALFAREGRYITPYGSLKIDELAELPCP
                     CRVCRSMTADELRQSEDRERLLALHNLHVTLAEIARIRQAIQDGTLWELVDERCRSHP
                     RLLAGYRELLAHAAELERDDPVSKRRFFYRGSETCRRTEVLRFHEVVPRIPLGERVLV
                     TFDGREVPGFDTVLNFKPPFGPYPVELAETFPIGQSEVPDWDDDMVRSGCAGIRALVE
                     AHPEKRFAIRCSDAWVRIVLEEVPGAEVYREQV"
     gene            12043..>13661
                     /gene="arcS"
                     /locus_tag="PLV90_05125"
     CDS             12043..>13661
                     /gene="arcS"
                     /locus_tag="PLV90_05125"
                     /EC_number="2.6.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002948 - archaeosine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0002927 - archaeosine-tRNA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosine synthase subunit alpha"
                     /protein_id="HOF96530.1"
                     /translation="MSRYEAQKRDGLARIGIYEEGGTTVSLPAALDTGALFPSLRERA
                     FSNVPLSADPAFVRDYFSPGEGQPVAVHPIAPPAVKSGDCALVANWHTALKNPRNYAT
                     WLAALKESVPPDAAWYAPGAALPSTACLLAYSGFDLFDYRAVDLASAQRIFCLPEGGF
                     PASIMATGVCGCEGCRDGDLIRHNRLALDRELALIRHYIEAGRLRELMESRCRADAAQ
                     VGILRFLDRNYALMERSLPVVRAVPMLAATAESQNRAEIRRFADRVIERFVPTRTDVA
                     VLLPCSARKPYSLSRSHRLFMNTVDRRAHELIVTSPLGLVPRELERVYPAGHYDVPVT
                     GYWDREECAFIADILARYFAAHPYRRVIAHLEGGALTVAEMAAEACGIELEVTCHGHP
                     TAPGSLRALAAALEGERRVQTDMVRGTVSWQFATDINTKGLQVRGRSMQMAVLRGKQQ
                     LFSIDAGTGLLRPTFEGWELIPEGYRVGIDAFVPQGDVLAPGITACDPRIREGDEVLV
                     EGPLAVATGRAMMGADEMLRSKRGVAVRVRKV"
BASE COUNT         2375 a         4538 c         4296 g         2352 t
ORIGIN      
        1 ccgagattcg atgaaaacaa tgagaaaaat cttctcgatg acaattcgat gctcagcaca
       61 gaaagtttat ggtcaggggt tcggacgtag tgcatgatgg tatcatgagc gtaacgaagt
      121 acagtgcgca ggttgttgaa agaatccttg ttgccgcact gcttgcctcc ctccttctag
      181 tctccggcgc ttcagcccta ccctgcacgg ccgccggcgg gattgcggta gctcccgccg
      241 acgatttgag cgacaggttg gagctcctcc ggtcagtcgg cggcacgata cacccgggcg
      301 agcaccagac catctggaaa tcggtgccgt cggacgtgag gaccttgcag gtctacctcg
      361 actggagcgg gcaccagcat cccggaaccg attctctcac cctgaccata accccgccgg
      421 gcaacggcgt gctcggcccg taccgtgacg gtgccgacgg gcggacggac gaaaagatcg
      481 ctctgactct ctccggatcc gatcccctgc cgtccggcat gtggaagttc ctgatcttcg
      541 gggaagacgt tccggaggag ggaacggagt atacgttgaa tgtagggtat tagtttcgaa
      601 ggagtccact accgatgcgg attgaactgt gtgggaggat actctcattc ttctttctag
      661 ccctggccct gttcgccctc ctctggagcc cggcggcagc ggcaaagatc atcgtcgaac
      721 cgggtccagc cgaactcccc acggacgtga cctacatcga cgacgagtgg ttcgtgccgg
      781 tctggcaggc ttactcaccg ttggaattcc tcaccatcgt cctcctcatc cactgcccgc
      841 tcctcgcgat gcccgtcgaa cttgtctact ccgccggcgt catggcttat ctcggttacc
      901 ggacctcacg cagccctctg gacaacacga accggcaccg gatctacgcc tgcatccggg
      961 accaccccgg gatcgcgccc gcggagattg cccgtgccac cgggatcaac cggggaacgg
     1021 tccattacca tctctcctgt ctccgggatg ccggcctggt cggtatcctg cgccgaaaag
     1081 gcaggggagg ctacttccga aacggcgact gtaatccaac tgagaaggct atgtacctgc
     1141 acctcaataa cgatatccgc cgacagatcc tcgccctgct cctcgaaaaa cccggcatca
     1201 cgcagtcgga gatagccgat attatgggca tcgcgcgctc cacggctgcc tggcacctgc
     1261 agaagctcgc cgcagacgaa ctcgtcgaat cggatcgcga cggtcggaca gtgcattacg
     1321 cactcacctg cgaggctcta gccttctcgg gggctctgag cagctggggg acactcttcc
     1381 ttcccgggga taaaacgagc gctggaaact gcgctgactc tctccctcag tagataccgg
     1441 ggtggatact ctcgttcgca ggggaatgag cgcgatcgac cggttcgcag gaaaactcgt
     1501 ttcggggaag catcatttta gtaaacacca cgcagggcga gacggaccgg tactcgatcg
     1561 atgttcccgc aggcaaaacc gaactcatcg ccgacctgaa ctggggcgac tcatccgact
     1621 cgctccggct gatggttcat gtgccggatg ccgtgctcgg gctgtaattt gcgcataaag
     1681 gactccggcg gcctgcaccc cgggatatgg cggttcgagg cctacgggaa cagagtagcc
     1741 ggcaccctgg actacacgct tatccggcga taaaagagcg aaacattgaa catctcttcc
     1801 atcggaccaa ccaccatggc gcggtggagc cggatacttc tcgtcctcat catcgccatg
     1861 atcctcgcac tcccgggcac ggcaaccgcc cgctacgtgg tcgagccggt ggcggaggaa
     1921 tatgccggcg agtggcacga tctcgaagag atcacgttcc gggatctccc gctccgggtg
     1981 atgctcctcc accttctgtt cacaatcctg cccggactcc cctatatagg ggaactcttc
     2041 tacgcgctca gccttcttgt gcctctcggt ttccggcggg tgatgcgcca taatatcctc
     2101 gacgatgacc tcagacgcga tctctaccgg cagatcaccg ctaacccagg tatcggaacc
     2161 acggagctcg gggagctgac gggtgcatca cgagggaggc tccgctacca cctcgatatg
     2221 ctcatcaggg agggcaaggt ggccgccgtg agctaccgaa accgtgccag gcacttcgca
     2281 ggaaaccagc ggtataccgc tctcgaagaa cgcatctttg ccagcctccg ggatgaaacg
     2341 ccggcggtga tactcacaca cctccacagg tcacccgggg caacccagaa cgagatcaaa
     2401 gatcaactcg ggcttgctgc tccgacgatc tcccagcata tgcaacagtt cgaggcagag
     2461 gggatcgtcg tcagagatca ggaggggaga tccttccgct acaggctcac cggagatgcc
     2521 cgggagatcc tcaacgagca ccagaaattc cccaccctga agagcgcatc gctgcgtaag
     2581 tggcgttctg tctcataatg tccagctcct cccgtgagaa ccctgtctta tactggccga
     2641 agatacacca gatcctcctg gcggtccccc ggcgctcctt gctgcacaga agcctgctgc
     2701 cccgaatcga cacccgggat aacatacgac ccggttgcgt tggagatgtt cctaatggga
     2761 agcacctcga tggccgatgt tccggggtgt tgcggcggcg ggcctgcggc gagccctcca
     2821 gccgtcgccc cgagaccgga aggagcaacg agatgacgaa gaagatcacg agggtgaaga
     2881 tcaatgcgtt gccgctctcc cggacggccg tcgagaatgc cagggcaacg gcaaaccagg
     2941 caaacaggaa gaggaacggg gccccgctca cttcagcaga tccgcaagtt gccgcatctc
     3001 ccggatgtgg tactgccctt ccgccgtcgg ggtgccggca tgacagaaga cgaactccga
     3061 cccgaagagc ggcaggacgg cccgggcgta gcggaacccg gcgcccatga tgctcgtgca
     3121 gatgggtttc tgagcctggt gggtgaacgt gacgagcgtc aggaggtcgt ctgcagtgcg
     3181 gggcttcacg acgatcttcg gtatgtcgcc gtatgacctg agcagggcct cgatcctgcc
     3241 gagttccggg ggggtgggca tctcgttcgt gtggagggat gcaacgatct ggacaccctg
     3301 gctcttgatg aacggcgcgt ggtcccggta acgggcctcc acgtccacgt agtcgacgta
     3361 gcgcgttacc ggcccgacga cccgcatcca gaggtccgca tccccgacaa accttccccc
     3421 ctcctcccgg ctcctgagcg tggcgatcag gggaatggcg gtcttctccc ggatcgcacg
     3481 gaccaggtcc gggaggtcgc cctccatccg gtcgagcctg acctcgatga acgtcgggtt
     3541 gtactccagc acctggtcga tgatgccggg ctcctccaca gagacgacga tcttcatact
     3601 tatcgggggt atgaccgctc atcgcttaaa ggtctgccca ctgtaccgta gcgctccagc
     3661 atcgcggcga agacctcgtc aaagaccgcc ggcaaccgtt cccggaaccc ccgcacgccg
     3721 acctcggtga tgccgccgcc ggtcgcaacc tcccctacca ggtcccccgg cgccctgccg
     3781 gtctcccgga ggtatgcacc cgtggccgcg accatctccg tcgcgagcag aagagcccgg
     3841 tcgggcggaa tcccgctcgc ccgaaccgcc gcgcccgcca gctcctcgat gagggaagcc
     3901 agcagtcccg gcccgcagct cgagagcagc gtcgccgccg gaagatcggc ctcactgacg
     3961 gccaccacgc tcccgatggc tgagaagagc ctctccaccc ggagagcgtt gtccatcccg
     4021 acctgccgcc cgtgacagac cagcgtcgtc ccctctccga ccgcggaggt gatcgtgggc
     4081 acgacccggg tgaccgggcc cgtatacgca tcctcgatcg ccgcaaggct gatgccggcg
     4141 gcaagcgaga cgatatgctc gtcgccggcc aacgccgggg cgatctcgca gaggacgccg
     4201 cccacctcct gcggcctgac ggcgatgatg accgtccggc accgccgggc aatgtccgcg
     4261 ttcgttgcgg cgatgccgac cccgggccac gctgcggcgc aggcatcaag gctccgccgg
     4321 ctccgggacg cgaccacgat ctcgtcggcg cggagtgccc cggacgtgat aaaccccttc
     4381 accagcatgc tgcccatgtg gccgtaaccg ataatccctg tgctgttcat cctttccctt
     4441 cacatcggcc tctcgcgcaa aaagaatact catcctcacc ggggtggagt atataggccg
     4501 gcacggcgaa cggtacccga caatgccgca cgatcaccct caggggaccg gcccggttaa
     4561 gcccccggag ctgcttgccc ccgccggatc gccggaagcc ctggctgccg cggtcgccgc
     4621 cggcgcggat gccgtctacc ttgccgggag gcgtttcggg gcccggcact acgcggcgaa
     4681 cttctccgac gaagagctcc ggtccgccat cgattacgca cacctccatg acgtgcgagt
     4741 tttcgtcacc gtaaacaccc tcgtccggga tgccgaactc tccgacgtgg cccggtacct
     4801 cctctggctc tacgaggccg gagccgacgc cgtcctggtg caggatgccg gggtggcgtc
     4861 gcttgcccgg gagatcgtcc cggaccttcc tctccacgcc tccacccaga tgacggtcca
     4921 caaccgcgag ggcgtggccc gggccgcgcg ggaggggttc tcccgcgtgg tgctggcgcg
     4981 ggaggtcacg ctcgccgaga tcgaggatat cgcagaggtg caggggatcg ggatcgaggt
     5041 cttcgcccac ggtgctctct gctactgcta ctcggggcag tgcctcctct cctcggtcat
     5101 cggaggccgg agcgggaacc gggggatgtg cgcccagccg tgcaggaaac cctaccggct
     5161 cgtgaccgcc gataaggacg attacggtcg cccgaagtcc ctccgggcgg tgcctgcaga
     5221 ggatcgctac ctgctctcga cccgggacct cgcggtatac ccgcacctcg atcgcatcgc
     5281 acgtgcatcg gtggcgtcgc tcaagataga ggggaggatg cgctccccgg agtacgtcgc
     5341 cacggtggtg gatatctacc ggcgcgccct cgacacgatc gccgccgggg ctccctggtc
     5401 gccctcgcgg gaggatatgc gggacctcgc cctcgcgttc aaccgggagt tcacggaggg
     5461 ctacatactc ggcgctcccg atatcatggc ccgcgaccgg cccgggaacc ggggcgtcct
     5521 cctcggcacg gtcagcggct acgacccgcg gaggcgggag gcgagcgtgc gcctcgccgg
     5581 cgatacctcg ccccgctctg gcgacgggct cgccttctct tccgacgacc ccgaccgcga
     5641 cgtgggggcg atcgtccgcg gcacccccgc cgtgaagaac ggcatggtcc gcatcggtgt
     5701 cggcgccccc gtcggggcgg gtgcccgtgt cttcctgacg aagagcgccg acctcgagga
     5761 gcgggcaaag cggatcatgg caaaccccct gccgcagatt cccatcgata tcctggtcac
     5821 ctgggagggc ggcaggccct ctctggaggc gacggttccc gtgtgcggcg gcgaaccgct
     5881 ccgggtgctc taccgtgccg atatcgtgat ggagcctgca cggagccggc cgcttaccgg
     5941 ggagcagatc gcagaacacc tcgggaagac cggggggacg ccgtttaccg tccggaagat
     6001 ggacctccgg taccccgggg gactctttac accgataggg gagttgaacc gcatccggcg
     6061 aacattctta gagagagtcg gggaggccgt gctcgccgca cggcgcccgg ggccggatgc
     6121 ggtgcatgca gcccgggagc gggtcgaggc agcgatcgaa gggatgacac gcacggcagg
     6181 atcgcgccgc acaccccgga ccccatcggt ctcggtctac gtcgataccc tcgaggctgc
     6241 agcggccgcc gcacgggagg gcgcccgcac gatctacatc gaaccttccg gacccgtggg
     6301 cctccgcttc ctcgaagagg cggccaccgc ctgccgggag gggggcgccg atctggtctg
     6361 gaagtggccg acggtcaccc ggcgcgggtt cctcgatgcg gcggcccctc tcctcccgtc
     6421 gctctacgat gcgggcatcc gcggggttat ggtgagcggg atgggggccg cggatgcggt
     6481 ccggaacgcc gaaccccgga tgcgcctcta cggcgccgcc gggctgaacg tctggaacca
     6541 ccgcactgcc ggttccctgt cggcgctctt cctgcggtgc acggtctccc ccgaactccc
     6601 ggccgccgat gttgcagaac tcgcggggtc ggtcgggaac accccggaac ttgaggtgat
     6661 cgtgcagggc accatcgagg cgatggtgac cgaggaccgg ctggtcgcag cggtcgccgg
     6721 cgaagaaccc ggcaatcgct tcctggggct cgatgaccag cgcaggcgca tcttccccct
     6781 caggcacgat agcgaagggc ggacctatat cgcgaatgcc gccgagatct gcctgatcga
     6841 tcgccttccc ggggtggccg ggatgggcat cgacgccgtc gccatcgatg cgcgggggcg
     6901 gggagcctgt tatgccgggg atatggtccg gctctaccgg gaggggatcg atgccgtgaa
     6961 caggggcgac accggaatcc tccccgcgct caaagatgag gtgagacggc gggcgctcgg
     7021 cggcatcacc ggcgggcact tcgtccgggg gctcgcggag tgaatctccg gggccgcccg
     7081 tcgattctcc ccctgccccg gcaccagagt aaatatcagg aggagcaacc tattggcaga
     7141 tgtgatggat atggtctcac ccttcattct tgtcaacctc aagacctacc aggaaggtat
     7201 gggcagcaac gcccaccgga tcgccgctgc ggccgaggcc gtggcaaggg agagcggggt
     7261 cgccatcggc attgcgccgg tcttcaccga gattcgcccg ctgagccagc attatgcgat
     7321 accggtttac gcccagcata tcgatgcagt cacccccggc gcccacaccg gtcacatact
     7381 ccctgaagcg gtcagggccg cgggcgcacg cggcaccctg atcaaccact ccgagcgccg
     7441 cctgactctc gccgatatcg acgcctgcgt cgagaacgcc cggaggctcc acctggaatc
     7501 ggtcgtctgc acgaacaacg acgcgaccag cgccgcagcc gcggccctcc ggcccgacta
     7561 cgtggcgatc gaacccccgg agctgatcgg gagcgggatc tcggtctcga aggccgaccc
     7621 ggggatcatc gagcgatccg tcagcgctgt cagggcggtg aacccggatg tgaaagtcct
     7681 gaccggggcg ggcatccagt cgggcgaatg cgtgaagatt gccgtcgacc tcgggacctg
     7741 cggcgtcctc ctcgcgtcga gcgtggtcaa ggccgccgac cccgaagcgg tcttcaggga
     7801 cctgatctcc ctgctctgat gccctacgtg atcagcgata tcaggtcgtg cttggtgatg
     7861 acaccctgca ccttcccctg ctcgaccacg atgaccgcgt ggttgtggtg caggagatgg
     7921 acgatcgtat cgatgggcgc cgtcggggga actgtcggga aacccggctc catgtagtcc
     7981 tgcaccagct tccggtgcgt ctggtggagc cctccctcct ccatggcgct catgatggcc
     8041 gattcggata tgcatcctac cggcaccccg ccctcgagca ccggcagctg ggagatgccg
     8101 ttctggccca tgatctcgac ggcacggttg acagggtcgt caggagtaac ggagagcacg
     8161 ggtgcggtca tgacggtggc cgccgtgacc gtcgagtgct ccgccgcctg caggacatcc
     8221 acgatctttg caagcgtgct caccctcggg tcgacgctgc ccgcctcgat ccgcgcgacc
     8281 atggattggc tgatccccgc cattcgggct acgtctgcct gttttaagcc catacgaagc
     8341 cttttttccc gcagttcagc cggggtgggt atctccatat tattactata ggtaattttt
     8401 ttcacttata cgtttgcccg ccggtgcggt catcggcggt atcgcgatcc gtcgcccctt
     8461 cccgggcagg ttgatatagg gcagggttcc actggatggt atggccgtaa acagggagca
     8521 acagcagaag gtcctcacga aggtccgcga gatcctctcg acctatcaca cccgcgatgc
     8581 cgtcaagatc gagctggagt ctcgtgggtt cactatcagg gcagagcacg gagatgtcgt
     8641 atccctggag aacacgcccg ccgagatctt cgtccagctc ttcgtgaatg accggggcga
     8701 catcgtcgat acgcacgtcg tgacgtttga ggagatcgag ctcaggccgc cggctaaaga
     8761 ataaactgcg ctctaccgag aagagttgca aggagtggga ggagtaagcc tccttctgtt
     8821 ctgtgcggca ttcagctacg cgccatacct gggcggatac cagacgatcc atctgcccta
     8881 ttctggcttg cacccgcagg ggttcaccgt ttcatcgtat cccgccgcta cgttcaacga
     8941 gtcgcgcgga aacccgcgca aagctcgccc ggagggctcg atcgtttcat cactgacggc
     9001 ggaccgtgtc gtttctgtgt cattgccgtg actctcgcca cccgcacttg catgcggctg
     9061 cgtgtctggc cggtgggagg actttcctca gcagcacaga gcatgtgcca ccgtcggccg
     9121 cactctccct ctcctatata ctatggtgcg gagcaaatat atatcagtac ggtgatccgc
     9181 ctccatcgtc ttgcactgcg gatcccttca aaagtcaccg gatcggccgc gcataccccc
     9241 gggcatgaga taggtttata taatcaacct ctgttcttac caccggcgtt atcaccatgg
     9301 ggggaagtga ggcatctaca cagctggagc ttgcggcaat tttcgcggcg ttcgttgccg
     9361 ttacgacgct cttctcgttc gtctctatcg gggcgaatgc tctcgggggt aaaatcccgg
     9421 agggtgcggg tgtatacccg gtcctggccg ccggcgaacc gcgtcttgcg ccggtcgggg
     9481 atatcatcgg ttatccatcg gtcccggggc catccggcac ccggatcgac gccctgacct
     9541 tcgtcatcgc caataccggc ggagaggggg cggtcgacct ctcgcgggcc accgtgacgg
     9601 tcatggcggg gaattacctt gaggttctca cgcgatcgga ggatctccac ccggaaccgg
     9661 gcgcgtggac gacgtcgctg cccggggaca gcgacggcaa cgtgctcctc cgggcaggcg
     9721 aggagtgtgc ggtccatatc cgccttgacc acctcatccc tgccggggag accgtgaccg
     9781 tcagggtgcg ccttcccggt gaccggccct gcctgatcgc cgggcaggcc ggccacccgg
     9841 aagactaaaa gtattcaaca tcatacgccc cattctgatg gaaatgctga cnnnnnnnnn
     9901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccggaagac taaaagtatt caacatcata
    10021 cgccccattc tgatggaaat gctgaccccc gaagaaggca gaacggtagt tcgtctggca
    10081 cggagcgcca tcgaaaagac cctcagcggc gagcgggcag tactgcccga tctcccggcg
    10141 gtctttcgcg agaagcgcgg cgtcttcgtt acgatcaagc agcagggaca tctccgcggg
    10201 tgcatcggcc tcccctaccc gatcaaaccc ctcggcgagg ctatcgtcga ggcggccata
    10261 tcggctgctc tcgaggaccc ccggttcccg ccggtatcgc ggtcggaggt tgccggtatc
    10321 gatctcgagg tgacggtgct cacagaaccc cggccgctcg actgcccccc ggatgaacgt
    10381 cccggttgcg tcgaggtcgg gagacacggc ctgatcgtca gcggtcacgg gacaggaggc
    10441 ctcctcctcc cccaggttcc ggccgagtac ggctggacca gccgggagtt cctcgaccag
    10501 acctgcatca aggccggcct tccgcccgga tgctggaagc gcggcagcgt cgccgtgctg
    10561 acattcgagg ggcagatatt cgatgaaaag gcggtttaat ctgaatgaca atcacgtttg
    10621 aggtcatcca caaggatata gcgggaaggg tcgggaaact cagggtaaac gataaggcgg
    10681 tccggacgcc ggcgctcctc ccggtcgtca acccgcacct ccccctggtg accccccgcg
    10741 agatgcagga gatgggcgtc gaggccctga tcacgaacgc ctacatcttc cggcggagca
    10801 cggagttttg cgaccgggcg cttgcggaag ggcttcacgc ggtcctcgac ttcgacggcg
    10861 ccgtcatgac cgactcaggc tcgtttcagc tctcggtcta cggcgaggtc gaggtgagca
    10921 accgtgagac gcttgagttc cagcaggcga taggaagcga tatcatcgtc cccctggata
    10981 tccccacccc gccggatgca agccgggaga gggccgaaca ggaactcgcg gtcacgatgg
    11041 accggatccg ggaggcacag gccctcttcc ccgacgcaaa cctcgcgggg ccggtgcagg
    11101 gcgggatctt caccgatctc cgcgaggagg ccgggcgtgc cgttcgggac ctcggcgtgg
    11161 ctttcgctcc gatcggggcc gtggtgccgc tgatggagaa ctaccgctac caggacctgg
    11221 tgagggtcgt cctcgcggcc aaacgcggcc tctccccggc gaccgcgatc cacctcttcg
    11281 gtgcaggcca cccgtcgatg ttcgcccttg ccgtcgcgat gggctgcgac ctcttcgact
    11341 cggcagcata cgctctcttt gcccgggagg ggcgctacat cacaccgtac gggagcctca
    11401 agatcgacga acttgcggag ctcccctgcc cctgccgggt ctgccgctcg atgaccgccg
    11461 acgaactccg gcagtcggaa gaccgggagc ggctgcttgc cctccacaac ctccacgtca
    11521 ccctcgcgga gatcgcccgc atccggcagg cgatccagga cgggaccctc tgggagctcg
    11581 tcgacgagcg gtgccggagc cacccgcgcc tgcttgccgg ctaccgggaa ctcctcgccc
    11641 acgcggccga actcgaacgc gacgaccccg tctccaagcg ccggttcttc taccggggct
    11701 cggagacctg ccggaggacg gaggtgctcc ggttccatga ggtcgtcccc cgcattcccc
    11761 tcggcgagcg ggtgctggtg acgtttgacg ggcgcgaggt ccccggattc gatacggtgc
    11821 tgaacttcaa gccccctttc gggccgtatc ccgtggaact cgcggagacg ttcccgatag
    11881 gccagagcga ggtcccggat tgggacgacg atatggtccg gtcgggatgc gccggtatcc
    11941 gggccctggt ggaggcgcac ccggagaaga ggtttgccat ccggtgcagc gatgcgtggg
    12001 taaggatcgt cctcgaagag gtccccggtg cggaggtgta ccgtgagcag gtatgaggcg
    12061 cagaagcgcg acggcctggc acggatcggg atctatgaag agggagggac gaccgtctcc
    12121 ctccccgccg ccctcgatac gggtgccctc ttcccgtcgc tccgcgaacg tgccttctca
    12181 aacgtcccgc tctccgccga cccggcgttc gtccgcgact acttcagccc cggcgagggg
    12241 cagccggtcg ccgtccaccc gatcgccccg ccggcggtaa agtccggcga ctgcgccctg
    12301 gtcgcgaact ggcacacggc actcaagaac ccgcggaact acgcgacatg gcttgcggcc
    12361 ttaaaagaga gcgtcccccc ggacgccgcg tggtacgccc cgggagcggc actcccctcg
    12421 accgcctgcc tcctcgccta ctcgggtttc gacctcttcg actaccgggc ggtcgacctc
    12481 gcctctgcac agaggatctt ctgcctcccc gagggcgggt tcccggcctc gatcatggca
    12541 accggggtct gcgggtgcga gggctgccgg gatggcgacc ttatccggca caaccgcctt
    12601 gcgctcgacc gcgagctcgc cctcatccgg cactacatcg aggccgggcg gctccgggaa
    12661 cttatggagt cgcggtgccg cgcggacgcc gcacaggtgg gcatcctccg gttcctcgac
    12721 cggaactacg ctctcatgga gcgctccctc ccggtggtgc gggcggtgcc gatgcttgcc
    12781 gccaccgccg agtcgcagaa cagggcggag atccggcggt tcgccgaccg ggtgatcgag
    12841 cggttcgtcc cgacccggac cgacgtcgcg gtcctcctcc cctgttctgc gcggaaaccc
    12901 tactcgctct cacggagcca caggctcttc atgaacacgg tggaccggcg ggcgcacgaa
    12961 ctgatcgtca cctcgcccct cggcctcgtg ccgagagagc tcgaacgggt ctatccggcg
    13021 ggccactacg acgtgccggt gaccggctac tgggaccgcg aagagtgcgc cttcatcgcc
    13081 gacatactcg cccgttactt cgccgcacac ccctaccgcc gggtgatcgc ccacctcgag
    13141 ggcggagcgc tcaccgtcgc agagatggcg gcggaggcct gcggcatcga gctcgaggtg
    13201 acctgccacg gtcacccgac ggcgcccggc tccctgcgcg cgctcgccgc cgccctcgaa
    13261 ggcgagcgcc gggtgcagac cgatatggtc cggggcacgg tctcctggca gttcgcgacc
    13321 gatatcaaca cgaaaggcct ccaggtccgg ggcaggagca tgcagatggc ggtccttcgc
    13381 gggaagcagc aactcttcag catcgatgcg ggaaccgggc ttttgcgccc gacgttcgag
    13441 ggctgggaac tgataccgga gggctaccgg gtcgggatcg atgcgttcgt gccgcagggc
    13501 gacgtccttg ccccggggat cacggcgtgc gatccccgta tacgggaggg cgacgaggtg
    13561 ctcgtcgagg gcccgctcgc ggtcgccacc ggccgggcga tgatgggggc ggacgagatg
    13621 cttcggtcga aacgcggggt cgcggtgcgg gtgaggaagg t
//
LOCUS       DAOSII010000048        13138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_4794_length_13138_cov_53.911259, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000048 DAOSII010000000
VERSION     DAOSII010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 13138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13138
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4794_length_13138_cov_53.911259"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..1731
                     /gene="thsA"
                     /locus_tag="PLV90_05130"
     CDS             67..1731
                     /gene="thsA"
                     /locus_tag="PLV90_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit alpha"
                     /protein_id="HOF96531.1"
                     /translation="MSSLGGQPILILKEGSQRTRGRDAQSGNIAAAKAVANAVRTTLG
                     PKGMDKMLVDTIGDVVITNDGVTILKEMDIEHPAAKMMVEIAKTQDDEVGDGTTTAVV
                     IAGELLKRAEDLLDQDVHPTVIAHGYRMAADRAQVILDEIAVDVKPDDMAMLKKLADT
                     AMTGKGAEAAKEKITDLVVRAITMVADADGTVDTEFVKVEKKVGGSIEESEIVEGMII
                     DKERVHPAMPRVVKNAKILLLNAAVEFKKTEVDAEISITSPDQLQMFLDEEERMIKGI
                     VDKIIASGANVLFCQKGIDDIAQHYLAKAGIFSVRRVKKSDMEKLARATGGAIVSSID
                     AIAPEELGKAGTVEEKKVSGEEMTFVTGCENPKAVSIIIRGGTEHVVAELDRAIEDAL
                     RVVSVAVEDKKLVAGGGAPEIELSLRLREYAATVGGRAQLAIEAFANALEIIPRTLAE
                     NAGLDPIDMLVALRASHEKGGANGKYMGLDVFNAVSGDMLKAGVVEPLRVKTQAVSSA
                     AEAAVMILRIDDVIAASKSAGPSPEEMAAMGGGMGGGMGGMGMPPM"
     gene            1989..3881
                     /locus_tag="PLV90_05135"
     CDS             1989..3881
                     /locus_tag="PLV90_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOF96532.1"
                     /translation="MTLRGSVRQALPFTIYLLLAVLLAMAPVIYLISFVGYTDVRQDL
                     MADTEEFRGQTEYGIVLAVNLVDAGLKLFDDTLNREMQEGFGPFAVEYERAGRDPGVM
                     DLARVKEELGGRMDLYVINENGIIEYTTYPPELGYDFKEIPAFYDRITDIRLNGAFSA
                     DRIVPELATGMLRKYAYQPSPDRRYLFELGQVVSESGRQRMALSYADAIREVADENPG
                     VSEIRVFDCLGERIVGAAHPDDDRRLGMVRKAYRERTMLEVENADAGELIRYLFVDRT
                     DSVHASDMSVVVELTYNTKFAEDTIAGMFAQHTRILLIGLSLIACLSAFAMYSLTRPV
                     RILVEDVDAVARGDLDRPIRVSGSEEFVHLGGSVSTMIASLKEILQRLQESEEKIIRH
                     NRTLEDEVGARTAALEESNRTASLLLDIMGHDINNANNIANLYSDILLTDLEGEPEAE
                     LLRKAKAGLAKSIGIVRNVNTIQEIQGKPGAIGKIDLDRIVRSEIRSLPDLRITYNGT
                     TAAVIADDLLSEVFSNLLGNAAKFGGPDIAIAIRIEEHEDDLIISIEDTGPGVPDAVK
                     PRVFNRLARGNGEAAGTGLGLYICRMLIARYGGRIWVDDRVAGHPESGTAVRFTLRKA
                     DEGGSP"
     gene            3878..5761
                     /locus_tag="PLV90_05140"
     CDS             3878..5761
                     /locus_tag="PLV90_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOF96533.1"
                     /translation="MTAVDGGRWHRRLGTHLLLAMILITLPVIGLISALDCNEVVGAL
                     MAGDELLREQTEKSVVRSVRLADAGLKLFDGTLDSRMAEGFDPVLAGYERAGRDPGAM
                     DLARIREELGEEMDIYVINESGIVEYTTYPPDIGLDFRKLPTFYDRITDIRLGDVFVA
                     DRVVTEPAGGILRKYAYQPSPDHRYLFELGLVCNATEAGRYAPDYRALREDLMRLNPA
                     LKEVRIYDSYGRLVNVTGSESPVVSVAVDPAAIAIFEGREGRTLANRTVGTITRYIFI
                     DLSDPASPSDKSLVVELTYTTTLLDARIADVQLGHITIVLFASLAACCIAIPVSRRIT
                     RPVQEIVDDADLIAGGDLDHRIRVSTGTEFARLSESIGIMVDSLKGNIRRLQESEEAL
                     RQYDARLEDMVRERTVELEESGRTANLFLDIMVHDINNANAIAIGYTQFLVDALEGER
                     KEMAQKMLSGLEQSSGIVGRVATLRKARESVTTLTPVDLDRVIRTEVAGHPAARIRYE
                     GRPVDVLADDLLPEVFANLIGNAAKFGGPDVAIAIRVEEREGEVTVTVEDTGPGIPDA
                     MKESIFGRFRKGEGSLAGAGLGLYICRTLIARYGGKIWAEDRVTGHPESGTAIRFTLR
                     TAG"
     gene            complement(5764..5958)
                     /locus_tag="PLV90_05145"
     CDS             complement(5764..5958)
                     /locus_tag="PLV90_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HOF96534.1"
                     /translation="MNGQNRAAVHPGMTVDVVLKRDQKTGKRTRGVVAEILTNSSYHP
                     HGIKVRLRDGQVGRVQEIIG"
     gene            complement(5975..7501)
                     /gene="lysS"
                     /locus_tag="PLV90_05150"
     CDS             complement(5975..7501)
                     /gene="lysS"
                     /locus_tag="PLV90_05150"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOF96535.1"
                     /translation="MSDMDHLCFDPTKIDKIRDLLERGVTVYPYTFERRDTVRNIKER
                     FSTIGHEKSAEEVSTAGRIYIVREHGKTLFADMGDSDGRIQLYLRKNDLGEEQFDLFR
                     QYVDAGDIIGVVGHAFRTKMGELTIWVDRFELLAKSICPLPEKFHGLKNVETRYRQRY
                     IDLIMNEESRETFRTRSRIISLLRQFLFERDYLEFETPTLQPVYGGANARPFTTHHNA
                     LDQKLYLRIAPELYLKRLVVGGFEKVFEIAKNFRNEDIDTNHNPEFSMVEIYEAYRDY
                     NDMMDLTEEILSFLAEKVCKSAIVPFAGHDLDLSLPWRRLTMEEAVREYAGIDVRAMS
                     LEELRAFGLAHCVEGCESAATPGEYLVLFFEHFGEKNLIQPTFVYDFPVENSPLAKKH
                     RTKEGMTERFELFIAGMEMANGFSELNDPLDQKARLELQDARRRKGDLEAQMIDYDFI
                     NALGYGMPPTGGVGIGIDRLVMLLTGKDSIKEVLLFPQMKTAVPGQNGDKTEESDGEE
                     "
     gene            7852..8469
                     /gene="udg"
                     /locus_tag="PLV90_05155"
     CDS             7852..8469
                     /gene="udg"
                     /locus_tag="PLV90_05155"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type-4 uracil-DNA glycosylase"
                     /protein_id="HOF96536.1"
                     /translation="MVRETGREVAIERLAAVISECRKCPLWENTHHAVPGDGDIRAGL
                     MLIGEAPGKQEDLTGRPFVGRAGKLLDGLLSGIGLTRDDVFIANIVKHRPPGNRDPRD
                     EEIRACTPYLEEQIRIIEPGVIVTLGRHSSRYILSFLAIEFDRITDIRGTVYPGSLFG
                     RPVRLIPTLHPAAALYNPAYREALAEDFAIARRELARTGGTAELD"
     gene            8476..8907
                     /locus_tag="PLV90_05160"
     CDS             8476..8907
                     /locus_tag="PLV90_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOF96537.1"
                     /translation="MTREKSYGAVVFRRDADLQYLILQYGAGHWDLVKGHGKRGESEK
                     ETVLRELAEETGIAQATFIPGFREEIHYFFQRRGRTVYKEVVYYLIETPEERVLLSDE
                     HIAYQWLPYGEALQAITFGNSKRVVSEAHEHLTMLQSRESG"
     gene            complement(8925..9260)
                     /locus_tag="PLV90_05165"
     CDS             complement(8925..9260)
                     /locus_tag="PLV90_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HOF96538.1"
                     /translation="MALETFIGVVGLVIGAFGAFFIVYGAMIAIVELLARETRIHERS
                     YHDIRGIFTTRILIGLEFFVAADVLKTILKPTFEDLIALGSIVAIRTVVSYFLGREAS
                     EIPEDRMER"
     gene            complement(9421..10191)
                     /locus_tag="PLV90_05170"
     CDS             complement(9421..10191)
                     /locus_tag="PLV90_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF96539.1"
                     /translation="MILEVDGVAFTYRSAPVIREVTFGVRPHQILAILGPNGVGKTTL
                     LKCMNAILKPKAGSIFVDEIDLLRADRMEIARKVGYVPQRCEAGRLTAFDAILLGRRP
                     HIRWDVTEADLRIVEAAIRMLDLEALSLRYIDEMSGGELQKVSIARALVQEPRVLLLD
                     EPTSSLDLKNQLEILGIVRSVVTDHDVAAVMTMHDLNMALRYADRFIFLKEGVIHAAG
                     GHEVVTPETIEAVYGVPVTVERYNGFRVVVPLASGSTC"
     gene            complement(10188..11300)
                     /locus_tag="PLV90_05175"
     CDS             complement(10188..11300)
                     /locus_tag="PLV90_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOF96540.1"
                     /translation="MHFADGTVPREYLGYVRRKHLWILGGIALLFILLVVSISVGAAG
                     VPIYDVFLSIVNGTADRIFVFLNPGATMSVSNVDRIVWNIRLPQALAAIVAGVGLSVA
                     GVAMQSILRNPLGSPFTLGISNAGAFGAAVSVILLGTGQMHSSVANAVTLNNPYLTTV
                     VAFIFCLLATAVILIISRVRGASPEVMVLAGVALSSLFTAGTMFLQFFASDTQLAAVV
                     FWTFGDVSRAGWQELGIMTFVVAAASIFFLANRWNYNAIDAGDETAKGLGVSVEAVRN
                     IGMVVAALVSAVIVSFLGVIGFVGLVCPHMVRRLIGDDQRYLIPGSCVAGGILLLGSD
                     TVARIIVAPYILPVAVLTAFLGAPVFIYLLLRGYRR"
     gene            complement(11327..12481)
                     /locus_tag="PLV90_05180"
     CDS             complement(11327..12481)
                     /locus_tag="PLV90_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HOF96541.1"
                     /translation="MMFFRYTRLLTAAALAALVLLMAAAGCTGTTAGGGAAGEEITVT
                     DGFGRSVTAPSPPESVVCSGSGCLRYLVYLQAQDLVVGVDDIEKKEQEAEGRPYALAY
                     GSKFKDLPLIGEFRGKDDPEKIISIGPDVVFKTGSTGTAYGTSAAEADKLEDKTGIPV
                     VAFPYGSLRDDAEKAEMYGGLRVMGEVLGKQDRAEEVIAYIDATMADLERRTGDIPAA
                     DQKSVYVGGISSAGAHGIISTEPAYPPFLWVHAKNVATGLGTAHADVAKEALVNWDPE
                     YIFIDAGTTQMESGGAVGELKNDAALAGLSAAKNGKVYGVLPYNFYNTNYETVLADAY
                     FVGKTLYPDRFADVDPVKKADEIYTFFVGKPVFGDLNGQYSNLGFAAIPL"
     gene            12783..>13138
                     /locus_tag="PLV90_05185"
     CDS             12783..>13138
                     /locus_tag="PLV90_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOF96542.1"
                     /translation="MPERMLTEPEGYGLLSAYGIPVPPHQVVTSAGDARAAAGRIGYP
                     VVMKIVSSDIIHKSDVGGVVTAIESPEGVEEAFRTIMQNGAARAPEAAITGIIVEKQV
                     PGGLEVLIGGKTDPSFG"
BASE COUNT         2589 a         4020 c         4143 g         2386 t
ORIGIN      
        1 aacgagtgaa tactattata tagaactaca aacctacctt tctatcgctt aacaacggtg
       61 atacctatgt caagtcttgg aggacaacca atccttattc tgaaagaggg tagccagcgt
      121 acccgcggtc gtgacgcaca gtcaggcaac atcgccgccg caaaggccgt cgcaaacgct
      181 gtacggacaa cgcttggccc taagggcatg gataagatgc tcgtcgacac catcggtgac
      241 gtcgtcatca caaacgatgg cgtgaccatc ttaaaagaga tggacatcga gcaccccgcc
      301 gcaaagatga tggtcgagat cgcaaagacc caggacgacg aggtcggcga cggcaccacg
      361 accgccgtgg tgatcgcagg cgagctcctg aagcgcgccg aggatctcct cgatcaggat
      421 gtgcacccca cggtcatcgc ccacggctac cggatggccg cagacagagc gcaggttatc
      481 ctcgacgaga ttgccgtcga cgtcaaaccc gacgatatgg cgatgctcaa gaagcttgcc
      541 gataccgcga tgaccggcaa gggcgccgag gccgccaaag agaagatcac cgacctcgtc
      601 gtcagggcga tcacgatggt tgccgatgcc gacggcaccg tcgacaccga gttcgtcaag
      661 gtcgagaaga aggtcggcgg atccatcgag gagtccgaga tcgtcgaggg catgatcatc
      721 gataaggagc gcgtccaccc cgcgatgccc cgcgtcgtca agaacgcgaa gatcctgctc
      781 ctgaacgccg ccgtcgagtt caagaagacc gaggtcgacg ccgagatcag catcacgagc
      841 cccgaccagc tccagatgtt cctcgacgaa gaggagcgga tgatcaaggg catcgtcgac
      901 aagatcatcg cgagcggtgc aaacgttctc ttctgccaga agggtatcga cgacatcgcc
      961 cagcactacc ttgcaaaggc cgggatcttt tccgtccggc gcgtcaagaa gagcgacatg
     1021 gagaaactcg cccgtgcgac cggcggcgca atcgtcagct ccatcgacgc catcgccccc
     1081 gaggagctcg gtaaggcagg cactgtcgag gagaagaagg tctccggcga ggagatgacc
     1141 ttcgtgaccg ggtgcgagaa cccgaaggct gtctcgatca tcatccgcgg cggcaccgag
     1201 catgtcgtcg ccgaactcga ccgtgccatc gaggatgccc tcagggtcgt cagcgtggcc
     1261 gtcgaagata agaaactcgt cgccggcggc ggcgcgcccg agatcgaact ctcgctcagg
     1321 ctccgcgaat acgctgcaac cgtcggcggc cgcgcccagc tcgccattga agcatttgca
     1381 aacgccctcg agatcatccc gaggacgctt gccgagaacg cgggcctcga cccgatcgac
     1441 atgctcgttg cgctgcgcgc atcccacgag aagggcggtg caaacgggaa gtacatgggc
     1501 ctcgatgtct tcaacgccgt ctccggggat atgctcaagg ccggcgtcgt cgaacccctc
     1561 cgggtaaaga ctcaggcggt ctccagcgcc gccgaggcag ccgtcatgat cctccggatc
     1621 gacgacgtca tcgccgcgtc caagtccgcc ggcccctctc ctgaggagat ggctgccatg
     1681 ggcggcggta tgggtggcgg catgggcggc atgggcatgc ccccgatgta aatcaccgta
     1741 ccgttccacg atacgatccg gggccaagat acggccccgg cattctcttt tttcttcgct
     1801 gccgggatat cgcgcccttc gctgcaatca ggcatgccgc cgctttctcc ggggttctct
     1861 gggcaaaaca accgataaaa ccccatttat cacatcaaaa tcgctcacac taaatacctg
     1921 caggtatacc tttttgaaag aatgttctgt gccctggggg aggcgcaaaa cgcggagtat
     1981 gtgcccggat gacgttgagg ggatcggtgc ggcaggctct cccgttcacg atctacctcc
     2041 tgcttgccgt cctgctcgcg atggcgcccg tcatctacct gatatcgttt gtcgggtata
     2101 ccgatgtccg gcaggacctc atggcggata cagaagaatt ccggggccag accgagtacg
     2161 ggatcgtcct cgcggtaaac cttgtcgatg ccgggctgaa actctttgac gataccctga
     2221 accgcgagat gcaggaggga ttcggtccgt tcgccgttga gtacgaacgg gcggggcggg
     2281 atccgggcgt catggatctt gcccgggtca aggaggagct cgggggccgg atggaccttt
     2341 acgtcatcaa cgagaacggt atcatcgagt acaccaccta tccgcccgag ctcgggtacg
     2401 acttcaagga gatccctgcc ttctacgacc gcataacgga tatccgactc aacggcgctt
     2461 tctcggccga caggatcgtc cccgagctcg ccaccgggat gctccgaaag tatgcctacc
     2521 aaccatctcc cgaccgccgc tacctcttcg agctcgggca ggtggtgtcg gagtccggaa
     2581 ggcagcgcat ggcgctgagc tatgcggatg caatccggga ggtagcggat gaaaacccgg
     2641 gcgtcagcga gatccgtgtc ttcgactgcc tgggcgagcg gatcgtgggt gcggcgcatc
     2701 ccgacgatga tcgccgactg ggcatggttc ggaaggcgta ccgggagagg accatgcttg
     2761 aggtggagaa cgccgacgca ggcgagctga tccggtacct cttcgtcgat agaacggata
     2821 gcgttcacgc atcagacatg agcgtggtcg tcgagctgac ctacaacacg aaattcgcgg
     2881 aagatacgat cgcgggtatg tttgctcaac acacccgaat cctgctcatc gggctttcgc
     2941 tcatcgcctg cctctctgcc ttcgccatgt actccctgac gcggccggta cgcatcctcg
     3001 tcgaggatgt cgacgctgtc gcccgcggcg acctggaccg ccccatacgg gtgagcggga
     3061 gcgaggagtt cgtgcatctt ggagggagcg tatcgaccat gatcgcatcc ttaaaagaga
     3121 tcctgcaaag actccaggag tcggaagaga agatcatccg gcacaaccgc accctcgagg
     3181 atgaggtcgg ggcacggacg gccgccctcg aggaatccaa ccggacggcg tcgctcctgc
     3241 tcgacatcat gggccacgat atcaataacg caaacaacat cgcaaacctc tactccgaca
     3301 tcctcctcac cgaccttgag ggtgaacctg aagcggaact gctccggaag gcaaaggcgg
     3361 gacttgcaaa gagcatcggg atcgtgcgca acgtcaacac catccaggag atccagggga
     3421 aaccaggggc tatcggaaag atcgaccttg accgaatcgt cagatccgag atcaggtcac
     3481 tccccgacct ccgtataacc tacaacggca ccacagctgc agtcatcgcc gacgacctcc
     3541 tctcagaggt cttttcgaac ttgctcggga acgcggcgaa gttcggtggg ccggacatcg
     3601 caatcgccat ccggatcgag gagcacgaag acgatctcat catatccatc gaggataccg
     3661 ggccgggtgt cccggatgcc gtaaaacccc gggtcttcaa ccgcctggcc cgggggaacg
     3721 gagaggccgc ggggaccggg ctcgggctct acatctgccg gatgctcatt gcgcggtatg
     3781 gggggaggat atgggtcgat gaccgggtgg cggggcaccc cgaatccggc acggccgtcc
     3841 ggttcaccct ccggaaggcc gatgagggag gtagcccatg acggcggtgg atggcggcag
     3901 gtggcatcgc cgtcttggca cacacctgct gctcgccatg atcctgatca cgctccccgt
     3961 gatcgggttg atctcggccc tggactgcaa tgaggtcgtg ggggccctga tggccgggga
     4021 cgagctgctc cgtgagcaga cggagaagag cgttgtccgg tcggtgcgcc tcgcggatgc
     4081 ggggctgaaa ctcttcgacg gcacactgga ctcccggatg gcggaggggt ttgacccggt
     4141 tctcgcgggg tacgagcgcg ccgggaggga ccccggggcc atggaccttg cccggatccg
     4201 cgaggagctc ggggaggaga tggacatcta cgtcatcaac gagtcgggca tcgtcgagta
     4261 caccacctac cctccggaca tcggcctcga tttccggaaa cttcccactt tttatgacag
     4321 gataacagat atccgcctcg gcgatgtatt cgtggccgat cgcgtcgtga cggagccggc
     4381 aggcgggata ttgcggaagt acgcctacca gccctctccc gaccaccgct acctcttcga
     4441 actcgggctc gtctgcaatg cgaccgaggc cggccgttac gccccggact accgggccct
     4501 gagggaagac ctgatgcggt tgaaccccgc gctcaaagag gtccggatct acgacagtta
     4561 cgggcggctc gtcaacgtga ccggatcgga gagcccggtc gtgtccgtgg cggtggatcc
     4621 ggccgccatt gctatctttg aggggaggga aggcaggacg ctcgcgaata ggactgtcgg
     4681 gactataacc cggtatatat tcatcgacct ctccgatccc gccagcccct cggacaagag
     4741 cctggtcgtc gagctgacct acaccaccac tctcctcgac gcccggatag cggacgtgca
     4801 gctcggtcat atcaccatcg ttctctttgc gagtctcgcc gcctgctgca tcgcgatccc
     4861 ggtctcgcgg cggatcaccc gcccggtgca ggagatcgtc gatgacgccg acctgatcgc
     4921 cggcggggat ctcgaccacc ggatccgggt ctctacgggg acagaatttg ctcgcctctc
     4981 ggagagcatc ggcatcatgg tcgactccct caaggggaac atccggcgcc tccaggagtc
     5041 ggaggaggct ttacggcagt atgatgcccg tctcgaggat atggtcaggg agcggacggt
     5101 tgagctggag gagtccggcc ggacggcaaa cctcttcctg gacatcatgg tccacgacat
     5161 aaacaacgca aacgccatcg ccatcgggta tacgcagttc ctggtcgatg cgctcgaggg
     5221 cgagcgtaaa gagatggcgc aaaagatgct ctcggggctt gagcagagtt cggggatcgt
     5281 cgggcgcgtc gccaccctcc ggaaggcccg ggagagcgtg acaacgctca cacccgtgga
     5341 tctcgaccgg gtgatacgga cagaggttgc cggccaccct gcggcccgga tacggtacga
     5401 gggccgtccg gtcgacgtgc ttgccgatga cctccttccc gaagtctttg caaacctcat
     5461 cggcaacgcg gcgaagttcg gcggaccgga tgtcgcaatc gccattcggg tcgaggagcg
     5521 tgaaggggag gtcacggtca cggtcgagga taccggaccc ggcatccccg atgcgatgaa
     5581 agagagcatc ttcggccgtt tcaggaaagg ggaggggtcg cttgccggag ccggactcgg
     5641 gctctacatc tgccggacgc ttattgcgcg gtatgggggg aaaatctggg cggaagaccg
     5701 ggttaccggg catcccgagt ccggcacggc catccggttt accctccgga cggccgggta
     5761 atcttacccg atgatctcct gtacccgccc gacctgccca tcgcggagcc ggaccttgat
     5821 cccgtgaggg tggtaggacg agtttgtcag gatctcggcg acaacgcccc gggtgcgctt
     5881 cccggtcttc tggtcacgtt tgagcacgac gtccaccgtc atgccggggt ggacggcggc
     5941 gcggttctgc ccgttcatgg gcggagatga ggattcactc ttccccatcg ctctcttccg
     6001 tcttatcccc gttctgcccg gggacagcgg tcttcatctg cgggaagagg aggacttcct
     6061 tgatcgagtc cttgccggtg aggagcatca ccaggcggtc gatccctatc ccgacgccgc
     6121 ccgtcggggg cataccgtag ccgagcgcgt tgatgaagtc gtagtcgatc atctgcgctt
     6181 cgagatcgcc ctttcggcgc ctggcatcct ggagctcgag ccgggccttc tggtcgagtg
     6241 ggtcgtttaa ctccgagaac ccgtttgcca tctccatccc ggcgatgaag agttcgaacc
     6301 gctcggtcat cccctccttc gtccgatgtt tctttgcgag cggcgagttc tcgaccggga
     6361 agtcgtagac gaacgtcggc tgtatgaggt tcttctcgcc gaagtgctcg aagaagagga
     6421 ccaggtactc tcccggggtc gcggcgctct cacagccctc gacgcagtgc gcaagaccga
     6481 acgcacggag ttcctcaagg ctcatcgccc ggacgtcgat gccggcgtac tcccgcaccg
     6541 cttcctccat ggtgaggcgg cgccagggga gggagaggtc gagatcgtgg cccgcaaacg
     6601 ggacgatcgc acttttgcac accttctccg cgaggaacga gaggatctcc tcggtgaggt
     6661 ccatcatgtc gttgtagtcg cggtaggcct cgtagatctc caccatcgag aactcggggt
     6721 tgtggttggt gtcgatgtcc tcgttccgga agttctttgc aatctcaaat actttctcga
     6781 acccgccgac gacaaggcgc ttgaggtaaa gttccggcgc gatccggagg tagagtttct
     6841 ggtcgagggc gttgtggtgg gtggtgaacg gccgggcgtt cgcgccgccg tagaccggct
     6901 gcagggtggg agtctcgaac tccaggtagt cccgctcaaa gaggaactgc cggaggagcg
     6961 atatgatcct gctccgggtc cggaacgtct cgcggctctc ttcgttcatg atcaggtcga
     7021 tgtagcgctg ccgatagcgg gtctcgacgt tcttgagccc gtggaacttc tcggggaggg
     7081 ggcagatcga ttttgcgagg agttcgaacc ggtcgaccca gatggtgagc tcccccatct
     7141 tcgtccggaa ggcgtggccg acgacgccga tgatgtctcc cgcgtcgacg tactgccgga
     7201 agaggtcgaa ctgctcttct ccgaggtcgt tcttgcgaag atagagttgt atcctcccgt
     7261 ctgagtctcc catgtcggcg aagagcgtct tgccgtgctc gcggacgatg tagatcctgc
     7321 ccgcggtgct gacctcttcg gcgctttttt cgtgcccgat cgtggagaac cgctctttga
     7381 tattcctgac ggtatccctg cgctcgaagg tgtaggggta gaccgtcacc ccgcgctcga
     7441 gcagatcccg tatcttatcg atctttgttg ggtcaaaaca gaggtgatcc atatcgctca
     7501 ttgtgtaaac agctccattt gtcatgcggc ccgatttcac atcccaaacc cgggtttgcc
     7561 gcttttccgg gaagaaagag ccgcaatctc attgataaat cctacatata cttccgctcc
     7621 ggtgctatta atctttgagg gagggtggat cgaggcggcg caggcacccg ggctgcagcc
     7681 cggaccccgt cgcaggccgc ctcccgcggc attacgcaca cgtgagaggg ccggccgctc
     7741 ccggtctgca ggcaatgggc ccggccggcg aacccctcca ggtccgccgt ctgccggcgg
     7801 caaaacgcaa tgcataagaa ggttatacga gttatcgtca atagtgtggg catggtgcgt
     7861 gagacgggaa gagaggtagc gatagagagg cttgccgccg tcatcagcga atgccggaag
     7921 tgccccctct gggagaacac ccaccacgcg gttccggggg atggcgacat cagggccggc
     7981 ctcatgctga tcggcgaggc accggggaag caggaagacc tcaccgggag gccgttcgtg
     8041 ggacgggcag gaaaacttct cgacggcctc ctctccggga tcggtcttac ccgggacgat
     8101 gtcttcatcg caaacatcgt caaacaccgg ccgcccggaa accgcgatcc ccgtgacgag
     8161 gagatacggg cctgcacccc gtatcttgaa gagcagatcc ggatcatcga gccgggggtg
     8221 atcgtgacgc tcggtcgcca ctcgagtcgc tacatcctct ccttcctcgc catcgagttt
     8281 gatcggatca cggatatccg cgggaccgtc taccccggct ccctcttcgg ccgcccggtt
     8341 cgcctcatcc ccaccctcca ccctgccgcc gcgctctata acccggcata ccgggaggcc
     8401 ctggcagagg atttcgcgat cgccagacgt gaactcgcaa ggacgggagg gactgcagaa
     8461 ctggattgaa cggccatgac gagggagaag tcatacgggg cggtggtttt ccggcgggat
     8521 gcggatctcc agtacctcat attgcagtac ggggccgggc actgggacct cgtgaagggg
     8581 cacggcaaac gtggcgagag cgagaaggag acggtactcc gcgaacttgc ggaggagacc
     8641 gggatcgccc aggccacgtt catcccgggg ttccgtgagg agatccacta cttcttccag
     8701 cgccgggggc gcacggtcta caaggaggtc gtctactacc tgatcgagac cccggaagag
     8761 agagtgctgc tctcggacga gcatatcgcc taccagtggc tcccttacgg cgaggcgctc
     8821 caggcgatca ccttcgggaa ctcaaaacga gtcgtcagcg aggcgcacga acacctgacg
     8881 atgctgcagt cccgggagag cgggtgaccg cgagacccag tacctcatcg ttccattcta
     8941 tcttccggaa tctcgcttgc ctctctcccg aggaagtaac tcacgacggt cctgatcgcg
     9001 acgatcgacc cgagggcgat cagatcctca aacgtcggtt tgaggatcgt cttcaagacg
     9061 tcggcggcga cgaagaactc aaggccgatc aggatgcgcg tggtgaatat cccccgtata
     9121 tcgtggtaac tcctctcatg tatgcgcgtc tcccgcgcga ggagttcgac gatcgcgatc
     9181 atcgctccgt agacgatgaa aaacgctcca aacgccccga tgaccagccc gacgaccccg
     9241 atgaatgtct cgagcgccat gcccggcggg tgtatcggca gcgggtggac ttataagtat
     9301 agcaatcgcc ggtatccgcg cgggagagga cagaggtggt aagagaggcc cgcgcacgag
     9361 acatcggggc ggcgtacggc ccggggccag gcatcacccc gatccgggcc ccggtgcgta
     9421 tcagcaggtg ctccccgacg caagggggac gacgacgcga aatccgttat agcgctcgac
     9481 cgtcacggga acaccgtaga ccgcctcgat ggtctcgggc gtcaccacct cgtgcccgcc
     9541 cgcggcatgg atgacgccct cctttaagaa gatgaaccgg tcggcgtagc ggagcgccat
     9601 gttcaggtcg tgcatcgtca tcaccgccgc gacatcgtga tcggtcacca cgcttctcac
     9661 aatcccgagg atctcaagct ggttcttgag gtcgaggctg ctcgtcggtt cgtcgaggag
     9721 gagtacccgg ggctcctgca cgagcgcccg ggcgatactc accttctgga gctcgccgcc
     9781 gctcatctcg tcgatatacc ggagcgagag agcctcgagg tcaagcatcc ggatagccgc
     9841 ctcgacgatg cggaggtccg cctccgtgac gtcccaccgg atatgcggcc gccggccgag
     9901 gaggatggca tcgaacgcgg tcaatcgccc cgcttcgcac cgctgcggca cgtaacccac
     9961 cttccgcgca atctccatcc ggtcggccct gaggagatcg atctcatcga cgaagatgga
    10021 tccggccttc ggtttcagta tggcgttcat gcacttcaag agcgtcgtct tcccgacgcc
    10081 gttcggtcca aggatcgcga ggatctggtg ggggcgcacg ccgaacgtga cctccctgat
    10141 gaccggggcg ctcctgtagg tgaacgccac gccgtcgacc tcgaggatca tcgccggtac
    10201 ccccggagga gcaggtagat gaagaccggt gcgccgagga acgccgtgag cacggcgacc
    10261 gggaggatgt agggcgcaac gatgatccgg gcgacggtat ccgaaccgag gaggaggatc
    10321 ccgcccgcca cgcaggagcc cggtatcagg tagcgctggt cgtcgccgat gagcctccgc
    10381 accatgtgcg ggcagaccag ccccacgaac ccgatgacac cgaggaacga gacgatgacg
    10441 gcggagacga gcgccgctac gaccataccg atgttgcgca ccgcttcgac gctgacgccg
    10501 agcccttttg ccgtctcgtc gccggcatcg atcgcgttgt agttccagcg gttcgcaagg
    10561 aagaagatgc tggccgccgc gacgacgaaa gtcatgatac cgagctcctg ccagccggcc
    10621 cggctgacgt cgccgaaggt ccagaagacg accgcagcga gctgggtgtc gctcgcaaag
    10681 aactggagga acatcgtccc tgccgtgaag agggaggaga gggcgacgcc ggcgagcacc
    10741 atcacctccg gcgacgcccc gcgcacccgg gatatgatca ggatcaccgc cgtcgcaagg
    10801 aggcagaaga tgaacgccac gaccgtcgtc aggtaggggt tgttcagggt gacggcattg
    10861 gcaaccgatg aatgcatctg cccggtgccg aggaggatga ccgagacggc cgcaccgaac
    10921 gcgccggcat tggatatgcc gagcgtgaac ggcgatccga gcgggttacg gaggatcgac
    10981 tgcatggcga cccccgccac cgagagcccg acaccggcga cgatcgcggc gagcgcctgg
    11041 gggaggcgga tgttccagac gatccggtcg acgttggata cgctcatagt ggcaccggga
    11101 ttgagaaaca cgaagatccg gtcggccgtg ccgttcacga tggagaggaa gacgtcgtag
    11161 atgggaacgc cggccgcgcc gaccgatatg gagaccacga ggaggatgaa gaggagggcg
    11221 atccccccga gtatccagag gtgctttctc ctcacgtacc cgagatattc ccggggaact
    11281 gtcccgtcag cgaagtgcat ctcgtcttat cctccgtata tcgcatttac agcgggatcg
    11341 cggcaaaccc gaggttgctg tactggccgt tcaggtcgcc aaagaccggt ttgccgacga
    11401 agaaggtgta gatctcatct gccttcttta ccgggtcgac gtcggcgaac cgatcggggt
    11461 agagggtctt tccgacaaag taggcgtcgg caagcacggt ctcgtagttc gtgttgtaga
    11521 agttgtacgg gagtacgccg tagaccttgc cgttctttgc tgcagagagg cccgcgagcg
    11581 ccgcatcgtt cttcagttcg ccgaccgcgc cgccgctctc catctgggtg gtgccggcgt
    11641 cgatgaagat gtactcgggg tcccagttga cgagcgcctc tttcgcgaca tcggcgtgcg
    11701 ccgtgccgag cccggttgca acgttctttg catgcaccca gaggaacggc ggatatgcgg
    11761 gttcggtgga tatgatgccg tgagcgcctg cagaggagat cccgccgacg tagacgctct
    11821 tctggtcggc cgcgggtatg tcgccggtcc ggcgctcaag gtcggccatc gtcgcatcga
    11881 tgtaggcgat cacctcttcg gcccggtcct gtttcccgag gacctcgccc atcacgcgga
    11941 ggccgccgta catctcggcc ttctcggcgt cgtccctgag cgagccgtag gggaaggcta
    12001 cgaccgggat acccgtctta tcctcgagtt tgtcggcttc tgcggcgctc gttccgtatg
    12061 ccgtcccggt cgaacccgtc ttgaagacga catcggggcc tatgctgatg atcttctcgg
    12121 ggtcgtcctt acccctgaac tcgccgatca gcgggaggtc tttgaacttt gaaccgtagg
    12181 caagggcgta ggggcgacct tctgcctcct gctccttctt ctcgatgtca tcgacaccga
    12241 cgacgaggtc ctgggcctgc aggtagacaa ggtagcgcag gcatcctgag cccgagcaga
    12301 cgacgctctc ggggggcgac ggggcggtga cactccggcc gaacccatcg gtgaccgtta
    12361 tctcctcgcc ggcagcaccc ccgccggcgg ttgtgccggt gcatccggca gcggccatga
    12421 gcagtacgag agccgcgagc gccgcggccg tgagcaatct ggtatacctg aagaacatca
    12481 tactgtttac tatgaatgta acactaatta atatacctga cgaaaggtaa cctatccagt
    12541 gttaagggat ttcgtttcgt agtacatatg cagacgtcaa agaacattcc tcagaacacc
    12601 atcgtcgacg cccccgtttg ggatcgtcgg ccattacggt cttttcacct tcctccggga
    12661 ggagaaaccg ggctcgctcc gccggccgga taactattat gccgggcagc cggaatagtt
    12721 gatagaaggg gaacgccggc catgcaaccc cggcatgacc attctcgcag cgaggaagag
    12781 agatgccaga acgaatgtta accgaaccgg aagggtatgg cctgctttcg gcgtacggga
    12841 tacccgtgcc gccccaccag gtggtcacca gcgccggcga tgcacgggcg gccgccgggc
    12901 ggataggcta cccggtcgtc atgaagatag tctcgtcgga tatcatccac aagagcgatg
    12961 tcggcggcgt cgtcaccgcg atcgagagcc ccgaaggggt agaggaggcg ttccggacga
    13021 tcatgcagaa cggcgctgcc cgtgccccgg aagcggcgat caccggtatc atcgtggaga
    13081 agcaggtgcc cggcgggctt gaggtgctga tcggggggaa gaccgatccc tcgtttgg
//
LOCUS       DAOSII010000049        12982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_4867_length_12982_cov_46.506459, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000049 DAOSII010000000
VERSION     DAOSII010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 12982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12982
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4867_length_12982_cov_46.506459"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLV90_05190"
     CDS             <1..186
                     /locus_tag="PLV90_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOF96543.1"
                     /translation="VKNLPLRFTEQYPEIPWRFIAGMRDKLIHHYFGVSLEIVWETVQ
                     SDMPVLREWLKGLKEEL"
     gene            complement(516..2132)
                     /gene="hcp"
                     /locus_tag="PLV90_05195"
                     /gene_synonym="priS"
     CDS             complement(516..2132)
                     /gene="hcp"
                     /locus_tag="PLV90_05195"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOF96544.1"
                     /translation="MFCNQCEETAKGFGCTVRGVCGKDEEAAGLQDVLIYTLKGLSTR
                     NLAAMKAGGGNPDAGKFVAGCLFATLTNVNFDPARLRDMINEAARIRDALPPAGSTEP
                     AACTWAPASPAAIAAKAQEIVAAREAVDPDLQSLRDLLVYGLKGLGAYYHHAAILGYE
                     DEEITVFMQEALAATLQDLSVGDMVGHVLACGEVGVKVLALLDTANTTAYGTPAITTV
                     RTTAGARPGILVTGHDLKDLADLLEQARGAGVDVYTHGEMLPAHAYPDLRKYDHLVGN
                     YGGSWPFQREEFERFNGPVLVTTNCLVPPKDSYRDRVYTTGLVGFAGLKHIDASPDGK
                     KDFSTLIEHAKRCKAPEDISRGDLVTGCAHAPVLALADTVIDAVKTGAVKRFLVMAGC
                     DGRYGGRDYYTRFAKALPADTVILTAGCAKYRYNSLGLGDIGGIPRVLDAGQCNDCYS
                     LVVIAKALAEAFGVGINDLPVSYNIAWYEQKAVLVLLSLLSLGVKDITLGPRLPAFVS
                     PGILKVLVENFGIRGIATVEEDLARMVPGN"
     gene            2218..2577
                     /locus_tag="PLV90_05200"
     CDS             2218..2577
                     /locus_tag="PLV90_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOF96545.1"
                     /translation="MQDECTVNQTVKYLGKKWTLLIILELYKGDGYTRRFTEMKERLP
                     GITAKVLSARLKELEEEGLVEKKIDTKTIPVRSEYTLTESGLGLVEVIKDIKRWALRW
                     KIENLACGAQDCRDCVL"
     assembly_gap    2918..3017
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3359..3676)
                     /locus_tag="PLV90_05205"
     CDS             complement(3359..3676)
                     /locus_tag="PLV90_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96546.1"
                     /translation="MEVLRTRIVIDWVRKDGLIFASDYSDCDHSAEGFRKVMFRVKGG
                     APLKAGAAYSLEYETEDILKPFVQKSRSGKTTSYYPHRFYHKIRNVTEVSANPDADLL
                     FDL"
     gene            3928..5553
                     /locus_tag="PLV90_05210"
     CDS             3928..5553
                     /locus_tag="PLV90_05210"
                     /inference="COORDINATES: protein motif:HMM:NF040080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6398 domain-containing protein"
                     /protein_id="HOF96547.1"
                     /translation="MASKVSPGTCLLCRAPLTKRKALKHGMACLQASGWPTGKKPSLL
                     IMIQGRYNKDYWLVVLARHDAWLRELDRLIRDVWVECCDHLSSFKIGGVAYESDSTFS
                     TGAMSVPLARLVSPGSTFTYDYDFGSTTSLDLKVIGETPIALPGGPLCLIARNDPPAI
                     PCDLCGGKAAVILDDPDDDIPHYYCRACLVSTEHDPDYVETIANSPRNGVCGYTEDIA
                     AALSWYPPGRSTGEVVSEEPDEVLPDDDAEVNAAIVAVIQDIGPDIDAFVQEEGAAHG
                     DRGACMAGDTILAFCTFMHLLSGTKIDAWDVPSVQRCLIEHLAQNLLTPDDWLKNAVP
                     ILCRFLSRMEASGRITNASELIAALKQAEPAFQEAATSPEKGRAIFEYILMKAEEAGI
                     SITDIDASFAFAVKELARMAGIDPDDEEVRKRLSGLLDSGITEAFSSKLRATMIFGRC
                     DEFCRRFKDDTILERCRRIFTALADHPAEPLLRGGGALWSAAIIYAACQDEDLIRPGK
                     GAPPLGQEIGFFFGFERSSIRNKVRAMRALLPE"
     gene            complement(5728..6816)
                     /locus_tag="PLV90_05215"
     CDS             complement(5728..6816)
                     /locus_tag="PLV90_05215"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HOF96548.1"
                     /translation="MSREEMDALARERLVGIARGWYDQMRDGIVPYIRLPTRTKQNIE
                     YDDESEVWKYGDKESTRSAATEKSAVHLLKMAYVIGFLKQQLAENRSSTLRELYYISE
                     GWKRAKFAAQDESNLLIEDLEIITEVQREAFHLRPEEDGASIFGPLRIREATRRGVKE
                     MHCQEDVGEAGYPIPNNVDNLDFVDHDAKFVIAIETGGMYARLMENGFDEEYGAILVH
                     LKGQPARSTRRVLRRLNESLNLPVVVFTDGDPWSYRIYASVAYGSIKSAHMSELLATP
                     QAQFIGVQPSDISDYNLPADHLTEQDINALKAELTDPRFATDYWRNQIALQLEMKVKS
                     EQQAFASRGLDFVTKEYLPKRLAEMGVI"
     gene            complement(6809..8611)
                     /locus_tag="PLV90_05220"
     CDS             complement(6809..8611)
                     /locus_tag="PLV90_05220"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HOF96549.1"
                     /translation="MVLAEDLAKQQRSISVAEFFEKNKHLLGFDSPTRGIITTIKEAV
                     DNALDACEEAEVLPDIFVGVRRVDADVYRITVEDNGPGIVPENVPPVFGKLLYGSRFH
                     QIRQSRGQQGIGISAAVLYAQLTTGTPALVVSRTGAKARAHRFELMIRTETNEPEIVS
                     HEEIDWDRMHGTRLEIQFKSTLAAKKRLVDYLRLTAIVNPHARITADIDGEVTTFERV
                     ASEVPPCPKPVLPHPHGIEFGALKRIAAASTGTIDEFLVGSFSRVGKKTADDMIALAG
                     LKPSRKVKNLDADELRRLLDAMQSVKVPAPPAQQCLSPIGEDLICRGLEKEIALDFVR
                     ARTRPSAVHGGHSFIIEAAIGYGGKIPAEGNAQIFRFANRVPLLYQQGACAITNCISQ
                     VNWKNYGLSQQGLPVGPALVMVHVASTNVPFTSESKDAVASIPEIEREIVLVLQELGR
                     ELKAYLSRRERKKQQDDRARAICSIIPEIAAKVSEIVELPLVDTSPIEGRIMRRVVAK
                     KRTHDGKVRIDVNNYTGGEVTLVLYNLSRDAATDANPRPDFVDEIDGEYNKVWRCTLD
                     AGAAWQVVYTGKGDGSLDLRGVEDGRKVVVDLDV"
     gene            complement(8598..9830)
                     /gene="hisS"
                     /locus_tag="PLV90_05225"
     CDS             complement(8598..9830)
                     /gene="hisS"
                     /locus_tag="PLV90_05225"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOF96550.1"
                     /translation="MLQKPRGTRDFLPDEMERRRLIERRMREAARRWGYREVSTPDFE
                     HLELFTMKSGEGIIQEMYVFEDKGGRQMTLRPEVTAAVLRMYVNEGKVLPKPIRWCYF
                     ADCFRYERPQKGRYRQFWQFGVELIGADTPSADAEVVMLADDVLRSTGVTFDLHVGHL
                     APMKHLLADLAPGDQRAIMACLDKHDTAGLEAALAERDLTHLAEPLAALSKCRSVSEV
                     FEIAGDIPERARIEEIFSLLDSQDIDYRPDFGIARGLDYYTGMVFEGFAKNLGAENQI
                     IGGGAYRLAHLFGGDDVASSGFAIGFDRVVVSIGDFPLVDEPIVGVVCTPEARARALD
                     VARAFRSAGVRAEIDLMQRGMGAQVSHAAKTADFAVILGKREVEAGTVTLRDLHSGKQ
                     QERSLEDAIAEVARHGSC"
     gene            complement(9847..10179)
                     /locus_tag="PLV90_05230"
     CDS             complement(9847..10179)
                     /locus_tag="PLV90_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF96551.1"
                     /translation="MSYLVYVAGFGVAVTVFLWLRDLRIFYRTGLSGYRTAAYWGVPY
                     TALALLGFFVTAYAEAWEFLGLGLVLLALYLQGRVDRENVWQNEGAAERFFGRAKRTK
                     DKGSRKRL"
     gene            10227..11000
                     /gene="larB"
                     /locus_tag="PLV90_05235"
     CDS             10227..11000
                     /gene="larB"
                     /locus_tag="PLV90_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOF96552.1"
                     /translation="MLPNATIREILRMYRSGEVSEDEAAAAIEGLRIDVLDGMARIDA
                     GRSVRCGMPEVVLAEGKDPLTFAEIMLAQVKAAGRCIATRVSPAHIEALRSRLPSDIR
                     LEHRKAARAVILSTGNGPDPRGGTVAILTAGTSDIPVAEEARLIAEEMGCDVRTAYDV
                     GVAGIHRLFSALKDLIPADVFIVAAGREGTLPAIVAGLVDRPVIGVPVSTGYGYMGGG
                     EAALASMLQACSVLAVVNIDAGFTAGAFAAQVAHGGGRR"
     gene            10997..11503
                     /locus_tag="PLV90_05240"
     CDS             10997..11503
                     /locus_tag="PLV90_05240"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide adenylyltransferase"
                     /protein_id="HOF96553.1"
                     /translation="MSRGFYIGRFQPYHNGHQSVLERIACSADEIVIGVGSAQLSHTV
                     ANPFTAGERVLMLTRSLADLPCPFYVIPIEDLQRNALWVAHVRAMTPPFETVYSSNPL
                     VMQLFAEAGVDVQSPDMYERETHSGTAIRRRMLDGEPWEHLVPQAVVDVVREIQGVER
                     LQRIAGSD"
     gene            11598..12851
                     /locus_tag="PLV90_05245"
     CDS             11598..12851
                     /locus_tag="PLV90_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96554.1"
                     /translation="MEETLALSFDDLPGWLGAREEEIDSGLLDATAPSREAITGALER
                     LREVIARMEAADADETIHPRLRDISKKALPQFTKSMAQILSREPSGDPETFYATAAET
                     LKSVLKAMKGQGKYLSSSYPDEMKEVRTAVRDLGREINAMTETVSRARADRQQVKDAR
                     EVYESLTRIRGEYTAASGQARDYRKALEELDGEIQKAEEDLAALRLRPDYARRQEIEG
                     MIRELDVQDEEIGREITALRTPAVHLFRKAEKAAGKAGEDTAAKAIGRALDAYTAPLQ
                     DDGDTLAGLIESVMPATLAMIRRGDLALKNQEEIGLFSDPDTLPAGIRRTMRRQREIR
                     EQRAALEETRAALPNVIDEQHLTAEVARLQREREVKAAARARAEGQQEISQTSYARER
                     ESLQSRATALAKREVMISAPDLPPT"
BASE COUNT         2416 a         4054 c         4024 g         2388 t
ORIGIN      
        1 gttaaaaatc ttccactccg gtttacggag cagtacccgg agatcccatg gcggttcatc
       61 gccggaatgc gggataaact catccatcac tattttggcg tcagccttga gatcgtctgg
      121 gagacggtgc agtccgatat gcctgtgctc cgggagtggt tgaagggact gaaagaagag
      181 ttatgagaca caggaactgt cactggatat gttgtgcgga gactgaacgc tgcgaagcga
      241 tctgaagccg gataaaggcg gtttgtccat accccgaggg cgtttctcgc actggcacat
      301 cccgggaaag ttataactca atcacgttaa gccgctgatg agcctgactg aacttttcct
      361 tctcatctac gctctggaag aagttcggga tctcttttag agggccgggt gccgttatca
      421 ggggtgatgg tgtcaggtgt gcaacgtaga taccgaaccg gagccccggg tgcggggtaa
      481 acctgcaaaa cctgcccgcc ccgaaaaaga ggtgttcagt tccccggcac catccgggcg
      541 aggtcctcct cgaccgtcgc gatcccccgg atcccgaagt tctcgacgag caccttgagg
      601 atcccgggcg agacgaaggc cgggaggcgg gggccgaggg tgatatcctt gacgccgagc
      661 gagaggagac ttaagaggac gagcaccgcc ttctgctcgt accaggcgat gttgtaagag
      721 accgggaggt cgttgatacc gaccccgaac gcttcggcga gggcttttgc gatgaccacg
      781 agcgagtagc agtcgttgca ctggccggcg tcgaggaccc gcgggatgcc gccgatatcc
      841 ccgagaccga ggctgttgta gcggtacttc gcgcacccgg cggtgaggat gaccgtatct
      901 gcaggaagag ccttcgcgaa ccgggtgtag tagtcccgtc cgccgtatcg gccgtcgcag
      961 cccgccatca cgaggaaccg cttgacggca ccggtcttca ccgcatcgat gaccgtatcc
     1021 gcgagggcga gaaccggggc atgggcgcac cccgtgacga ggtcgccgcg ggagatgtcc
     1081 tccggagcct tacagcgctt cgcatgttcg atcagggtcg aaaagtcctt cttcccgtcc
     1141 ggtgacgcat cgatgtgctt cagtcccgca aacccgacga gcccggtggt gtagacccgg
     1201 tcgcggtagg agtccttcgg ggggaccagg cagttcgtgg tcacgaggac cgggccgttg
     1261 aaccgctcga actcctcccg ctggaagggc caggagccgc cgtagttccc gaccaggtgg
     1321 tcgtacttcc gaagatcggg ataggcgtgc gccgggagca tctcgccgtg agtgtaaaca
     1381 tccacacccg ccccgcgcgc ctgttcaagg aggtcggcga ggtccttgag gtcgtggccg
     1441 gtgacaagga tacccggccg cgcgcccgcc gtggtccgga cggtcgtgat cgccggcgtc
     1501 ccgtaggcag tggtgttcgc ggtatcgagg agggcaagga ccttcacccc gacctcgccg
     1561 cacgcgagaa catggccgac catatccccg acggagagat cctgcagcgt ggcggcgagg
     1621 gcctcctgca tgaaaaccgt gatctcctcg tcctcgtagc cgaggatggc ggcgtggtgg
     1681 tagtaggcgc cgagcccttt gagcccgtag accaggaggt cacgaagcga ctgcaggtcg
     1741 gggtccacgg cctcccgcgc ggcgacgatc tcctgcgcct ttgccgcgat cgccgccggg
     1801 cttgccggcg cccaggtgca ggccgcgggc tccgtgctcc cggcgggcgg gagggcgtcg
     1861 cggatgcggg ccgcctcatt gatcatatcc cggaggcggg caggatcgaa gttcacgttg
     1921 gtcagggtcg caaagaggca tcccgcaacg aacttcccgg catccgggtt cccgccgccc
     1981 gccttcatcg ccgcaaggtt cctcgtcgag agccccttga gggtgtagat caggacatcc
     2041 tggagtccgg cagcctcctc atccttgccg cagacgccgc gtacggtgca gccgaaaccc
     2101 tttgccgtct cttcacactg gttgcagaac attcatctca ctccatacta tctttttgat
     2161 ataaggtatc aattagatac taaagtataa ggaggagaag gtttctctaa ttataccatg
     2221 caggacgagt gcacggtcaa tcagaccgtc aagtatctcg ggaagaaatg gacgctcctg
     2281 atcatcctcg agctctacaa gggggacggt tacacccggc ggttcaccga gatgaaggag
     2341 cggctcccgg ggatcacggc aaaggtcctc tcggcccgcc tgaaggagct ggaggaggag
     2401 ggcctcgtcg agaagaagat cgataccaag accatccccg tcaggagcga gtacaccctc
     2461 accgaaagcg ggctcggact cgtcgaggtg atcaaggata tcaagcggtg ggcgctccgg
     2521 tggaagatcg agaacctcgc gtgcggggcg caggactgcc gcgactgtgt tttgtgaacg
     2581 accctgccct cgcggacggg actttgcacc aatcattccc cggcgggtcc accggatagc
     2641 atgcacgctt gctctcacag gcgggctgcc ccggctggcg gggatacccc gcatcaccgc
     2701 tttgccaccg gcggcagcgg ccggaaccgt gccggcgccc ccgccgcaag tatcgtgttt
     2761 gaccgcctcc ggcatgaagg ttagccgtgc ggggggattc gtgagccaga cgaaggggca
     2821 ccgctcccat caccggcatg cgcaggggga gttcctcgga cgctctctcc ggggcatagg
     2881 gaatcgtaga tcataatcga cgcttttatt gattatcnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnctc tctccggggc atagggaatc gtagatcata atcgacgctt
     3061 ttattgatta tcatcgagta tactcgacac ttctattgat taccagtaac tactcagggc
     3121 acccgatgag tcgccgtcgg cgtgaagagt gttctctcag ttccggtaat acccattctc
     3181 ctgatcaccg aatggctgga aaaactgtga cgcctctccg gaacctgtgt gcgcgctccg
     3241 gtagcatgaa ctgccgggcc gccacagggt tgccggccgt tcagaagacc gctcctccgg
     3301 attcgggatg ctgtaccgct ccatacacca tgcccggagg atgcgattcc tggagggatc
     3361 agaggtcgaa caggagatcc gcatccgggt tggccgatac ttctgtgacg tttctgatct
     3421 tgtgatagaa cctgtgggga tagtaggaag tcgtcttccc gctccggctc ttctgcacga
     3481 agggtttgag tatatcctcc gtctcgtact caagcgagta cgccgctccg gccttgaggg
     3541 gagcccctcc ctttacccgg aacatgacct tccggaaacc ctccgctgag tggtcgcaat
     3601 ccgagtaatc cgaggcgaag ataagcccat ccttccggac ccaatcgatc acaatcctgg
     3661 tgcgcagcac ttccatcacc aatcctatat tgaattgctg ttgtcctcat caggctttaa
     3721 ggcgcgccgt gcggtgtgaa agtgatcgta gcgaaagtgc ctcgcccata agggatagcc
     3781 ggtgaagttc ccaaaacagt cactaacgtt catcaaaccc actgaacgcg gtagtccggg
     3841 catgcggggg tgcccgggtc gtatcctccg gggaggcggg caggcccacg atcattcatt
     3901 atccctgact atcatacctt ctggtgaatg gcatcaaagg tctcacccgg tacctgcctg
     3961 ctctgcaggg ctcccctgac aaaacggaag gctctcaagc acggcatggc ctgcctccag
     4021 gcgtcgggct ggcccaccgg aaagaaaccc tccctcctca tcatgataca ggggcggtac
     4081 aacaaagact actggctggt cgttctcgcg cgacacgacg cctggcttag agaactcgac
     4141 cggttgatcc gcgatgtatg ggtggagtgc tgcgaccatc tcagttcctt caagatcggg
     4201 ggagtcgcct acgaatcgga cagtacattc tccacaggcg ccatgagcgt ccccctcgcg
     4261 cgtctcgtct ccccgggcag cacgttcacc tatgactacg actttggctc caccacatcg
     4321 ctggacctga aggtgatcgg ggagacgccc attgccctgc cgggcggccc gctctgcctg
     4381 atcgcacgga acgatccgcc ggctatcccc tgcgatctct gcggcggcaa ggccgctgtt
     4441 atcctggatg atcctgacga cgacatcccg cactattact gccgcgcatg cctcgtatcg
     4501 accgaacacg accccgacta cgtggagacc atcgccaact ccccaagaaa cggcgtctgc
     4561 ggctataccg aggatatcgc cgccgcactc tcctggtatc cgccggggcg gagcacgggc
     4621 gaggtcgttt cggaagaacc ggacgaggtc ttgcccgacg acgatgcgga ggtgaacgcc
     4681 gcgattgttg ccgtcatcca ggatatcgga cccgatatcg atgcgtttgt ccaggaagag
     4741 ggggcggcac acggggatag gggcgcctgt atggccgggg acactatctt ggccttctgt
     4801 acgttcatgc atctcctctc tggaacaaag atcgacgcat gggatgttcc gtccgtgcag
     4861 aggtgcctga tcgaacatct ggcgcaaaat ctgctcactc cggatgactg gctgaagaac
     4921 gccgtcccca tcctctgccg gttcctctcg cgcatggagg catccggccg catcaccaac
     4981 gcctccgaac tcattgccgc cctcaagcag gctgaacccg ccttccagga ggcagccacg
     5041 agcccggaga agggtcgggc catcttcgag tacatactga tgaaagcaga ggaggcaggc
     5101 atcagcataa ccgacataga tgcatccttc gccttcgcag tgaaggagct tgcccggatg
     5161 gccgggatcg accctgacga cgaagaggtt cgaaagaggc tctccggcct gctcgatagc
     5221 ggaataacag aggcattctc cagcaaactg cgcgccacca tgatatttgg gaggtgcgat
     5281 gagttctgca ggcggtttaa ggatgacacc atccttgagc gttgccgaag gatcttcacg
     5341 gctctcgctg accatccggc cgaaccgctc ctgcggggag gcggggcact ctggagcgcg
     5401 gcgatcatct atgcggcctg ccaggatgaa gacctgatcc ggccgggaaa gggcgcccct
     5461 cccctcgggc aggagatcgg tttcttcttc ggctttgagc ggtcatcgat ccggaacaag
     5521 gtgagggcga tgagagcgct cctccccgag tgaaaccggc cggaagcaga actcccggca
     5581 acgaaggttg ccggccgccg gccctctgcc tgcggcagca tgacgctcaa tcgctccccg
     5641 tatgcgagag agcgcgtcgt cccggagggg gccaccgcaa aacctccatg gaagttacag
     5701 ggtaccggag aaccgccggg gtccgcctca gataaccccc atctccgcaa gcctcttcgg
     5761 caggtactcc ttcgtcacga agtcgagccc ccggctcgcg aacgcctgct gttccgactt
     5821 caccttcatc tcaagttgca gggcgatctg gttcctccag taatctgtcg caaaccgcgg
     5881 atcggtcagt tccgctttca gcgcgttgat gtcctgttcg gtgaggtggt cggcggggag
     5941 gttgtagtcg gatatatcgg agggctgcac cccgatgaac tgcgcctgag gggtcgcgag
     6001 aagttccgac atatgggcgc tcttgatcga gccgtaggcg acgcttgcgt atatccggta
     6061 cgaccagggg tcgccgtcgg tgaagaccac caccgggagg ttgagcgact cgttgagccg
     6121 ccggagaacc cgccgcgtcg agcgcgccgg ctgccccttg aggtggacaa ggatcgcccc
     6181 gtactcctca tcaaacccgt tctccataag acgggcgtac ataccgccgg tctcgatggc
     6241 gatgacgaac ttcgcgtcgt ggtcgacgaa atcgaggttg tcgacgttgt tcgggatggg
     6301 gtagcccgcc tccccgacat cctcctggca gtgcatctcc ttcaccccgc gccgggtcgc
     6361 ctctctgatc cgcaagggcc cgaagatcga ggcgccgtcc tcctccggcc ggaggtggaa
     6421 cgcctcccgc tgcacttcgg tgatgatctc gagatcctcg atcaggaggt tgctctcgtc
     6481 ctgggcggca aacttcgctc tcttccaccc ttcggatatg tagtagagct ctctcaaggt
     6541 cgacgaccgg ttctcggcaa gctgctgctt tagaaacccg atgacgtagg ccatcttcag
     6601 gaggtggacc gcgctcttct cggtcgccgc cgaccgggtg ctctccttat cgccgtactt
     6661 ccagacctcg ctctcatcgt cgtactcgat attctgtttc gtccgcgtcg ggagccggat
     6721 atagggcacg atgccgtccc gcatctggtc gtaccacccg cgggcgatac ccaccagccg
     6781 ctcgcgcgca agtgcatcca tctcttctct agacatcgag atccaccacc actttcctgc
     6841 cgtcctcgac accacgcaga tcgagcgacc catcgccctt ccccgtgtag acgacctgcc
     6901 aggccgcgcc tgcatcgagc gtgcaccgcc agaccttgtt gtactccccg tcgatctcgt
     6961 ccacgaagtc cggccggggg ttcgcatcgg tggccgcatc gcgggagagg ttgtagagga
     7021 cgagcgtgac ctcgccaccc gtgtagttgt tcacgtcgat acggaccttc ccgtcgtgcg
     7081 tccgcttctt tgcgacgaca cggcgcatga tcctcccctc gatcggggat gtatccacca
     7141 ggggaagttc gacgatctcg ctcactttcg cggctatctc ggggatgatc gagcagatcg
     7201 cccgggcccg atcgtcctgc tgcttctttc tctcccggcg ggagaggtag gctttcagtt
     7261 cccggccgag ctcctggagg acgagcacga tctcgcgctc gatctcgggg atggaggcga
     7321 ccgcatcctt gctctcgctc gtgaagggga cgttcgtgga tgccacgtgg accatgacga
     7381 gcgccgggcc cacggggagt ccctgctgcg agagaccgta gttcttccag ttcacctggg
     7441 atatgcagtt cgtgatagcg caggcgccct gctggtagag gagcgggacg cggtttgcga
     7501 accggaatat ctgggcgttc ccctctgcgg ggatcttgcc cccgtagccg atcgcagcct
     7561 cgatgatgaa cgagtgcccg ccgtggaccg cgctcgggcg ggtccgggcc ctgacgaagt
     7621 caagggcgat ctccttttct agcccccggc agatcagatc ctccccgatg ggggagaggc
     7681 actgctgcgc cggcggtgcc ggaaccttca cgctctgcat ggcgtcaaga agcctcctca
     7741 gttcgtcggc atcgaggttc ttcaccttcc gggagggttt gagcccggca agagcgatca
     7801 tatcgtccgc ggtcttcttc ccgacccggg agaaactccc gacgaggaac tcgtcgatcg
     7861 tcccggtgct cgcggccgcg atccgcttca atgccccgaa ctcgatcccg tgcgggtggg
     7921 ggaggacggg tttcgggcac ggcgggactt cgctggccac ccgctcaaac gtcgtcacct
     7981 cgccgtcgat atcggccgtg atacgcgcgt gcgggttgac gatcgccgtc aggcggagat
     8041 aatccaccag ccgcttcttc gcggcaaggg tgctcttgaa ctggatctca agcctcgtgc
     8101 cgtgcatccg gtcccagtcg atctcctcgt ggctcacgat ctcgggctcg ttcgtctcgg
     8161 tccggatcat cagctcgaac cggtgtgctc tcgccttcgc tcccgtccgg gagacgacga
     8221 gcgccggggt gccggtggtg agctgcgcat acagcaccgc cgccgatata ccgataccct
     8281 gctgcccccg gctctgccgg atctggtgaa accgcgagcc gtagaggagc ttcccaaaga
     8341 ccgggggaac gttctccggc acgatcccgg ggccgttgtc ctcgacggtt atccggtaga
     8401 cgtcggcgtc gaccctccgt acgccgacga atatgtcggg gaggacctcg gcctcctcgc
     8461 aggcgtcgag cgcgttgtcc accgcctcct tgatggtggt gatgatcccg cgcgtcgggg
     8521 agtcgaagcc gagaaggtgc ttgttcttct cgaagaactc cgcgacgctg atgctccgct
     8581 gctgcttcgc caggtcctca gcaagaacca tgccgtgcta cctcagcgat ggcgtcctca
     8641 aggctcctct cctgctgctt gcctgagtgg agatccctga gggtcaccgt ccccgcctcg
     8701 acctcgcgct tcccgaggat cacggcgaag tcggccgttt ttgcggcgtg agagacctgg
     8761 gcgcccattc cccgctgcat gaggtcgatc tcggccctca cccctgcgct acggaaggcg
     8821 cgggctacat cgagcgcccg tgcccgtgcc tcgggcgtac agacgacgcc gacgataggt
     8881 tcatctacga gcgggaaatc cccgatcgag accacgaccc ggtcgaaccc tatagcaaac
     8941 cccgaggagg ccacgtcgtc gccgccgaag aggtgggcga gccggtaggc gccgccgccg
     9001 atgatctggt tctccgcacc gaggtttttc gcgaacccct caaagaccat ccccgtgtag
     9061 tagtcaagtc cccgggcgat gccgaagtcc ggccggtagt cgatatcctg ggagtcgagg
     9121 agcgaaaata tctcctcgat ccgcgcgcgt tcggggatgt cgccggcgat ctcaaagacc
     9181 tccgaaacgc tgcggcactt cgagagcgcc gcaaggggtt cggcaagatg ggtgaggtcc
     9241 ctctcggcaa gcgccgcctc gagccctgca gtatcgtgct tgtcgaggca ggccatgatc
     9301 gcccgctggt cgcccggcgc gaggtcggcg aggagatgct tcatcggcgc gaggtggccg
     9361 acgtggaggt cgaacgtgac gccggtcgat cggagcacat catcggcgag catgaccacc
     9421 tcggcgtcgg cggacggagt atctgcaccg atgagctcga cgccgaactg ccagaactgc
     9481 cggtaccggc ccttttgtgg gcgttcatag cggaaacagt cggcgaagta gcaccagcgg
     9541 atgggcttcg ggaggacctt gccctcgttg acgtacatcc ggaggaccgc tgcggtcacc
     9601 tccggccgga gcgtcatctg cctcccgccc ttgtcctcga agacgtacat ctcctggatg
     9661 atcccttcgc cggacttcat cgtgaagagc tcaaggtgtt cgaagtcggg tgtgctgacc
     9721 tcacggtatc cccaccggcg ggccgcctcc cgcatccgcc gctcgatcag tcgtcgccgt
     9781 tccatctcgt cgggtaaaaa gtcccgtgtt ccccgtggtt tctgaagcat tcggtattct
     9841 cctgaactaa agtctcttgc gcgaaccctt atcttttgtg cgctttgccc tgccgaagaa
     9901 ccgctcggcg gcgccttcgt tctgccatac gttctcccgg tctacccggc cctggaggta
     9961 gagggctaac aggaccagcc caagccccag gaactcccac gcttcggcgt acgcagtcac
    10021 gaagaaaccg agcagggcaa gcgccgtata ggggaccccc caatatgccg ccgtccggta
    10081 gccggataga ccggtccggt agaatatccg caggtcgcgg agccacagga agacagtgac
    10141 ggcaacgccg aaccctgcga cgtagacgag gtaggacatg agatgaagta atattatact
    10201 tccccaccta tttttgttat cgaaccatgc tgccgaatgc caccatcagg gagatcctcc
    10261 ggatgtatcg tagcggtgag gtatctgagg acgaggcggc ggccgccatc gaggggctcc
    10321 gcatcgacgt gctcgacggg atggcccgga tcgatgccgg gcggagcgtg cgctgcggga
    10381 tgcccgaggt ggtgctcgcc gagggaaagg atcccctcac gtttgcggag atcatgctcg
    10441 cgcaggtgaa ggcggccggc cggtgcatcg cgacgagggt ctcgccggcg catatcgagg
    10501 ccctccggtc ccgcctgccg tcggatatcc ggctggaaca tcggaaggcg gcgcgggcgg
    10561 tcatcctctc gaccggcaac ggacccgacc cccggggcgg aaccgtcgcc atcctcaccg
    10621 cggggacatc ggatatcccc gtcgccgagg aggcgaggct gatcgccgag gagatggggt
    10681 gcgacgtcag gactgcgtac gacgtcggcg tggcggggat ccaccgcctc ttctcggcgc
    10741 tcaaggatct tataccggcg gatgtcttca tcgtggccgc cggccgggag ggaacgcttc
    10801 ccgcgatcgt cgccggcctc gtcgaccgtc cggtgatcgg cgttcccgtg agcaccggct
    10861 acggctacat gggcggcggc gaggcggcgc tggcgagcat gctccaggca tgttcggtgc
    10921 ttgcggtcgt gaacatcgac gccgggttca cggcgggcgc gtttgccgcg caggttgcac
    10981 acgggggcgg acggagatga gccgggggtt ctacatcggg cgattccagc cctatcacaa
    11041 cggtcaccag tcggtgctgg agcggatcgc ctgctctgcc gacgagatcg tcatcggggt
    11101 cgggagcgcc cagctctccc acacggtggc aaaccccttc acggccggcg aacgggtgct
    11161 gatgctcacc aggtcgcttg ccgaccttcc gtgcccgttc tacgtcatcc cgatcgagga
    11221 tctccagaga aacgctctct gggtcgccca tgtccgggcg atgacaccgc cgttcgagac
    11281 ggtctactca tcaaaccccc tcgtcatgca gctctttgcc gaagcagggg tggatgtcca
    11341 gtcgcccgat atgtacgagc gggagacgca ctcgggcacc gccatcaggc ggcggatgct
    11401 cgatggcgaa ccctgggaac acctggtccc ccaggccgtc gtggacgtcg tccgggagat
    11461 ccagggcgtg gaacgcctgc agcggattgc ggggagcgac tgacggaccc ttgataggga
    11521 aagccgccag aggatatacc gagcaagggt gaatcatgtt caatcgactg cgagatctct
    11581 ttaaaaagac cgaacctgtg gaagaaacgc tggccctctc ctttgacgat ctcccgggat
    11641 ggctgggtgc ccgcgaggag gagatagact caggccttct ggatgcaacg gcaccatccc
    11701 gggaggcgat caccggcgcc cttgaacgcc tccgggaggt gattgcccgg atggaggcgg
    11761 cagatgctga tgagacgatc cacccccgac tcagggatat atcgaagaag gcgctccccc
    11821 agttcacgaa gtcgatggcc cagatcctct cccgcgagcc ttcgggcgac ccggagacct
    11881 tctatgccac cgccgccgag accctgaaga gcgtgctcaa ggcgatgaaa gggcagggaa
    11941 agtacctctc ttcctcctat ccggacgaga tgaaggaggt gcgcactgcc gtcagggatc
    12001 tcggaagaga gatcaacgcg atgacggaga cggtctcccg ggcacgtgcg gaccgacaac
    12061 aggtgaaaga tgcccgggag gtgtatgaat cccttacccg tatccgtggg gagtacaccg
    12121 ccgcctccgg tcaggcgcgg gactacagga aggcactgga ggagctggac ggagagattc
    12181 aaaaggccga agaagacctt gccgccctca ggttgcgtcc ggactacgcc cggcggcagg
    12241 agatcgaggg aatgatccgg gaactcgatg tccaggatga agagatcggg cgcgagatca
    12301 cagccctccg aacccccgcc gtccatctct tccggaaggc cgagaaggcg gcaggaaagg
    12361 ccggagagga tacggcggcg aaagcaatcg gccgggccct cgacgcatac actgcccctc
    12421 ttcaggatga cggggatacc ctggccggcc tgatcgaatc ggtgatgccg gcaacgctcg
    12481 ccatgatccg gcgaggggac cttgccctca agaaccagga ggagatcggc ctcttctccg
    12541 accctgacac ccttcctgcc ggaatacggc ggaccatgcg ccggcaaaga gagatccggg
    12601 agcagcgcgc ggcgctggag gagactcgcg ccgccctccc gaacgtcatc gatgaacagc
    12661 acctcacggc tgaggttgcg agactgcaac gggagcgcga ggtgaaggct gccgcgcggg
    12721 cccgggcgga ggggcaacag gagatctcgc agacctcgta tgcccgggaa cgcgagagcc
    12781 tccagtcccg cgcaaccgct ctcgcaaaga gagaggtcat gatcagcgcg ccggatctcc
    12841 cgcccacctg aacaggaggg gtaatgttct catcaaaaaa ggaaaccctg tttatgcccg
    12901 gcgaccaacc attccatgag taatcgctgg tcaactaccc ctccctaaag ggaggggctt
    12961 gcgaacggct aagcgagacc aa
//
LOCUS       DAOSII010000050        12690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_4998_length_12690_cov_43.038227, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000050 DAOSII010000000
VERSION     DAOSII010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 12690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12690
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4998_length_12690_cov_43.038227"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..1040)
                     /locus_tag="PLV90_05250"
     CDS             complement(216..1040)
                     /locus_tag="PLV90_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOF96555.1"
                     /translation="MPRFAVSTMFFHEYPCDHIFDYIAESGLDSLEFWVETPHFWLAG
                     RPEGDLSCCIADHPDLSPITIHAPSLDLNPCSINPEVAAISVDYAVESVRMADRMGAA
                     VVTVHPGRRTAKRPPSAYDYQRFEKYVARLREAASEARVKVAIENLEPRVNALLTTAE
                     DAAEVLEREPWLRFTLDMGHAMMTSCDEVLRFIDLCIERMVNVHVSGLGENGRPHYPI
                     HDDPDAMRVLTELADRGYDGYLTLELEDMVFPGALSSEEKIVVLKRQLETFEKVFS"
     gene            1083..2327
                     /gene="xseA"
                     /locus_tag="PLV90_05255"
     CDS             1083..2327
                     /gene="xseA"
                     /locus_tag="PLV90_05255"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOF96556.1"
                     /translation="MLSGPAADPDQPGGLIFGVSEVSTLICDLLDDARLQEIWVRGEV
                     TNYKNHSSGHHYFSLSEGGERSSALINCVMWRSCAAELRFAPRNGMGVLAFGTLEVYE
                     PHGRYQLIVRRLLPAGAGERHLMVERWKRELDAEGLFSPERKRPLPAFPRRVGVVTSR
                     TGAALQDILSVISRRYPVEVVLSPAAVQGEGAHADIAEAIRRIDGLVDVIIVGRGGGS
                     FEDLFPFNHPDVVRAVAACRTPVISAVGHEVDTALCDFAADLPAPTPSAAAERAVPDR
                     VDLLREVAGHRERMRALLSHRIAAAENEVDDLRARMHPRRLERRVHERMQRLVEHADL
                     LRRAALARVQRERAALAEARAELLGRDPLAALDRGYCIVGSGRRVMRSARDLHPGDRV
                     MLLMKDGRCRAVIEEVTYDEDV"
     gene            2311..2496
                     /gene="xseB"
                     /locus_tag="PLV90_05260"
     CDS             2311..2496
                     /gene="xseB"
                     /locus_tag="PLV90_05260"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOF96557.1"
                     /translation="MTKTFEEMLEELRGIVRRLEDGDASLEESISIYERGALLVKQCE
                     DLLGAAEMKLTELGRDR"
     gene            complement(2471..4369)
                     /locus_tag="PLV90_05265"
     CDS             complement(2471..4369)
                     /locus_tag="PLV90_05265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF96558.1"
                     /translation="MHPPTPALDDLLEALDHRIRAVGEDAAPGGDLPGSGLCDLIDTL
                     GARIRSIDAGYRRCRHRLALLPAGCLCTGPDGVILEANRAAGALLGPAPDRLRGLSLP
                     AHLHPGSLSAFRSAVAALERGDEVPVQEFLLARPDGSTLPVGAAVAVVRDGAIEHLWI
                     LSDVSGQKMIEEARRRSEERYRELTGNISDAFLALDQEDRIISWNRVAANLSGHPGEE
                     VIGKSIYDVYPDLRTEEVVGFFREVRETGRPGMSECRFHVGGRDHAFEVRAVPTREGI
                     SIYIRDITDRRNAEEALRKSEERYRAIVENQTEMIYRRLPDGTITFANDAYCRSIGIP
                     PDDLVGRRYAPAIPADDQARIQKSLHSLTPAHPVLAVEHRVVMPDGEIRWQQWTHMAF
                     FDETGSLVEYQSVGRDTSAARMAEEALRLANKKLNLLSDVTRHDTLNRLNVLTGYIDL
                     SREETKDPDLQEYYRKEGEIVREIRRYMVFTGEYREIGVAAPIWQDVGRIAREAAAGL
                     DMGEVAVEVQVADIEVYADPLIVQVFANLIDNSLRHGGGVARIRISARESDRGVTIIY
                     EDDGIGIPLAEKEDIFERGFGLFLAREILSITDLSLRETGRPGEGARFEIGVPRGYYR
                     FTGPGQAP"
     gene            complement(4488..4913)
                     /locus_tag="PLV90_05270"
     CDS             complement(4488..4913)
                     /locus_tag="PLV90_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF371 domain-containing protein"
                     /protein_id="HOF96559.1"
                     /translation="MKARDIVRARGHPLVRGAHPTTFEVTRDETLTATGDCIIGIGAD
                     KGAADLDPGLKAVLRDGRAVLTTRLTAGGVTVEVRSRGSAALTLDHPADLVWRRSDFI
                     SDRTVGIRSDHTAATLPREFIEALRRGEDLVVELEAESP"
     gene            4947..5537
                     /locus_tag="PLV90_05275"
     CDS             4947..5537
                     /locus_tag="PLV90_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HVO_0476 family zinc finger protein"
                     /protein_id="HOF96560.1"
                     /translation="MMSIVCPACKEECEHQVLREAAELVVQCSVCGQIHRIPKPPEPR
                     IFTVKAIVSRETESQVCSVEMLEDEVVSLGDHIAAECGDEVYGVEVTGIEAGEKRVRR
                     AKAEEVTTLWTRGIEQVVVKASIHSGRTTIPLYQAAEGEDEFVVGEVYTFGGRRIRIS
                     HIKLRDGPVMRKEGWKTVASRVKRIYGYLEGRYPRT"
     gene            complement(5818..6096)
                     /locus_tag="PLV90_05280"
     CDS             complement(5818..6096)
                     /locus_tag="PLV90_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOF96561.1"
                     /translation="MAIKVINFYQEGCMGCEEQAPILHEVEQNLGITIEEIDAVKNPH
                     YIKEYNLRVTPTTLVIEDDRVRERMEGLVHREDLETAIRRHLPRPLPG"
     gene            complement(6142..6831)
                     /locus_tag="PLV90_05285"
     CDS             complement(6142..6831)
                     /locus_tag="PLV90_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96562.1"
                     /translation="MKKTQQKTPKGDDKAWLKWGYVGIALLIAVAMVGTYFAPVFGSS
                     QKAQIGNNAAIGYTIRGEDGRPIITTDQNLFSSEYEKGNLVLLTRGLTMPVGGLVSGE
                     NVATLPVEYPPDFGDFGLLGFETNAISEGLVGMRPGEVKVVSFSYGENNLEMNLSQED
                     VDGIGLNFTERQVGDVVALGLTTSPDISMGNDTATPALRFGKVTEKTADSLTIVYRYG
                     SAEITLNSITG"
     gene            complement(7023..9593)
                     /locus_tag="PLV90_05290"
     CDS             complement(7023..9593)
                     /locus_tag="PLV90_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOF96563.1"
                     /translation="MKTHDVSEERDTWYAFGLARLSAGEVDAAIRCFDRALEIDPAFA
                     EAARMREKALLNPGSAVGVAELPPRYEAAPEKEDEAGVGAAPPVSRRRSRKTAKILRF
                     LRTDKHCRDKVAHIERLPSHKPACGIVSPPLPGPLAAYLEQKGIRLYTHQCEAIERIR
                     GGNHIILTTPTASGKTLAFNIPIFEILLNNPRATALYIYPIKALANDQYKTLKEFEAA
                     TRIIVNAERFDGDTPGDERDRIRCTSKVILTNPYELHYMLGTHRRWASFFAGLKCIVV
                     DEAHTYRGVFGSNVALVFRRLERICAHYGSDPQFILSSATIANPEEFGGKLVGRPVVH
                     ISRDGSPRGSKHFVLYNPKPEGAKEDTVALSVHNETVKLLAACVRMETQTLCFARSRQ
                     LVELVALWTKEALRDDYPHLAQRVRSYRGGYLPEDRLRIEQQIKDGSLLGIVSTNALE
                     LGIDIGSLDCVILSGYPGTMMSTWQQAGRAGRRATESVAIMVAYANPLEQHFTRHPED
                     FFARPHENAVLDLENRYIRADHILCAAQELPLEPGRDREAFGDTIMETFRELAARGDL
                     RQTGAGWTYAGSETWPHGSVRLNSISSDSFLLMHGHRLLETLDPAQAFREAHPGAVFL
                     HDGQTYIVDEFDPEKRVIRVRYEETDYYTRAAREVSLRVTRPGARTRAGSIDIHTGDV
                     VISESYTGYRVIKHEKVINEISLDLPPLHFETRATWFSIPVSPNEPGLSGAAGFERGL
                     RGLENVIPTILPYFIMCDAHDINVHAGYRDAETGAPTVYVYDNFVGGIGLSEKVAGLL
                     PDILAMACRLVADCRCESGCPSCIYTSSYMSEGDVDKRATRVLLERLRDTLLQAQIPS
                     "
     gene            complement(9664..11244)
                     /locus_tag="PLV90_05295"
     CDS             complement(9664..11244)
                     /locus_tag="PLV90_05295"
                     /inference="COORDINATES: protein motif:HMM:NF024928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HOF96564.1"
                     /translation="MSVKDPGGASRRDVLRKLRMQFVQGDIEVERLAKEAVSEPQRFR
                     GIAGSGKTHILCQKAAYMHCEQPDLRIVYTFFTRSLYEYIEYWVQFYCRMYGGEFDPE
                     NPHSTLKILHAWGSAGRTGFYRMLCDHYDTDFFSPEDLRGDRHQTPNELFARACKSFL
                     QIQESRNLPLDPLFDAVLIDEGQDLVVDREDLLYRGKQPFFWMAYQSLCPPDPDRPEK
                     RRLIWAYDEYQNTSSIKIPSSREIFGEEFKLQKSVIMRKCYRTPAPVLMAAHAIGMGL
                     FFREGMLAGPTTKAEWNALGYDVEGEFKSGRQVRITRPEEYTANIVPDLWKGHLIDLQ
                     IVDRREEEASFVARSIQRDIVVSGLRPEDILVVSLNARSNAGVRSVAEELMERGIETY
                     ISSASGRNRLDIGAKPDTFSETGCVTVSGINRAKGNEGNVVYILGFDIIGRNDESIPL
                     RNSLFVALTRSRAWVTLTGTGRYPIYEEMKTILEHLQSTGGLTFTYRSRPKVAFDAGL
                     DMEPEGVSYQQILLYSDE"
     gene            complement(11557..12399)
                     /locus_tag="PLV90_05300"
     CDS             complement(11557..12399)
                     /locus_tag="PLV90_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6293 family protein"
                     /protein_id="HOF96565.1"
                     /translation="MSMTTKNDPLPGGLRDVVHIIPLGHEIDRAVAPFTKNKADRAYI
                     LAVPPDAGLDPRMVRKQHYFVGRVTEQLQDLGIAVTFVPVAMFDILDVLKVVSYLIRT
                     EKEAGNAVYVNMSSCGRKTSVAVTLAAMVHEAMLYYVSADRYATADGEECEHGLSIVK
                     TVRTEVFQPFRIMMPRPENVRLLVELFRRKEEGGMTSEEIIEFFHGANTHGYERLHTA
                     ERDGYQRAKKKRALLNRTNRGYLRELEEQGYVEREWRGRHFSIRITKAGEHIACVSGL
                     VPGG"
     gene            12477..>12690
                     /locus_tag="PLV90_05305"
     CDS             12477..>12690
                     /locus_tag="PLV90_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96566.1"
                     /translation="MRTNLQGLAGRRGTAVFRQYGACRGRGCAGRSILLGQVRDLADR
                     ARINYTAGFDAVGDLRRGDIVRFTAAI"
BASE COUNT         2146 a         3937 c         4171 g         2436 t
ORIGIN      
        1 gcggactttc gcccgggtta tcgatataag ggcgacctca gaacttgaga agaaggccga
       61 gaggagcaga cagatgataa agagtatgat ctctacggtg acaaggtcta cgattaccat
      121 ttactccacg ccgcaccaag cggcaacatc cggttgtccg gttacctgct gaatatatga
      181 attatgttgc aggcaggggt tataaaccca gcgcttcagg agaagacctt ctcgaaggtc
      241 tctaattggc gtttaaggac cacgatcttc tcttcagacg agagtgcccc gggaaagacc
      301 atatcctcga gttcaagggt caggtatccg tcgtagccgc ggtcggcaag ttcggtgaga
      361 acccgcatcg catccgggtc gtcgtggatg gggtagtggg gccgcccgtt ctctccgagt
      421 ccgctgacgt ggacgtttac catccgctcg atacagaggt cgatgaaccg gagcacctcg
      481 tcacaggacg tcatcatggc gtgccccata tccagggtga accggagcca gggctctcgt
      541 tcgagcacct cggccgcatc ctccgccgtg gtgagcaggg cgttgacccg gggctccagg
      601 ttctcgatcg ccaccttcac tcgcgcctcc gacgccgcct cccggagccg tgcgacatac
      661 ttctcgaacc gctggtagtc gtaggcgctc gggggccgtt tcgccgtccg tctcccgggg
      721 tggaccgtga ccacggcggc gcccatccgg tcggccatcc ggacggactc gacggcgtag
      781 tcgaccgata tcgcggcgac ctcggggttg atggagcagg gatttaagtc gagcgacggg
      841 gcgtggatgg tgatggggga gaggtccggg tgatcggcga tgcagcagga gaggtcgcct
      901 tcggggcggc cggcaagcca gaaatgcggg gtctcgaccc agaactcgag gctgtcgagg
      961 cctgattcgg cgatgtagtc gaagatatgg tcgcaggggt actcgtggaa gaacatggtc
     1021 gagacggcga aacggggcat aatattactt gatataaacc atcggtctaa tagattgtga
     1081 tgatgttgag cggtcctgcc gccgatccgg atcaacccgg gggcctgatc tttggagtct
     1141 ctgaggtctc gaccctcatc tgcgacctcc ttgacgacgc ccgcctccag gagatctggg
     1201 tgcgggggga ggtgaccaac tacaagaacc actcatccgg ccaccactac ttctcgctct
     1261 cggagggagg ggagaggagt tcggcgctga tcaactgcgt catgtggcgt tcctgcgcgg
     1321 cagagctccg ctttgcaccc agaaacggca tgggcgtgct tgccttcggg accctggagg
     1381 tctacgaacc gcacggcagg tatcagctga tcgtcagaag actgctcccg gcaggcgccg
     1441 gcgagcgcca cctcatggtc gagcgctgga agcgggagct cgacgccgaa gggctctttt
     1501 cgccggaacg aaagcggccc ctgccggcct tcccgcgcag ggtgggggtg gtcacctcgc
     1561 ggaccggcgc agcgttacag gatatcctct cggtcatatc ccggcgctac ccggtcgagg
     1621 tggtcctctc cccggcggcc gtccaggggg agggggcgca cgcggatatc gcggaggcga
     1681 tccggcggat cgacgggctc gtggatgtga tcatcgtcgg tcggggcggc gggagctttg
     1741 aggacctctt ccccttcaac caccccgacg tcgtcagggc cgtcgcagcg tgcaggacgc
     1801 cggtgatcag cgccgtcggc cacgaggtgg ataccgccct ctgcgacttc gccgcggacc
     1861 tcccggcgcc gacgccgtcg gctgcggcgg agcgggccgt gcccgaccgc gtggacctgc
     1921 tccgggaggt cgccgggcac cgcgagagga tgcgggcgct cctctcccac cggattgccg
     1981 cagcggaaaa cgaggtcgat gacctgcggg cgcgcatgca cccccggcgg ctcgaacgcc
     2041 gggtccacga gcggatgcag cgcctcgtcg agcacgcgga cctgctccgg cgcgcggcgc
     2101 tcgcccgggt gcagcgggag cgcgcggcgc tcgcggaagc ccgtgcggag cttctgggga
     2161 gggatccgct cgcggccctc gaccggggct actgtatcgt cgggtcggga cggagggtca
     2221 tgcggagcgc ccgcgacctc catcccggcg accgggtgat gctgctgatg aaggacggcc
     2281 ggtgcagggc cgtcatcgag gaagtgacat atgacgaaga cgtttgaaga gatgctcgag
     2341 gagctgcgcg ggattgtccg gcggctggag gacggcgacg cgagcctcga ggagagcatc
     2401 tccatatacg agcgcggtgc gctcctggtg aagcagtgcg aggacctcct cggcgcggcc
     2461 gagatgaagc tcacggagct tggccgggac cggtgaaccg ataatacccc cggggaacgc
     2521 cgatctcaaa ccgcgcaccc tccccgggcc ggccggtctc ccggagagag agatcggtga
     2581 tcgagaggat ctcccgggca aggaagagcc cgaacccccg ttcgaatata tcctctttct
     2641 cggcgagcgg tataccgatg ccgtcgtcct cgtagatgat ggtgactccg cggtctgact
     2701 cgcgggcaga gatccgtatc cgggcgacgc ccccgccgtg ccggagcgag ttgtcgatga
     2761 ggtttgcaaa gacctggacg atgagggggt cggcatacac ctcgatatcg gcgacctgca
     2821 cctcgacggc tacctctccc atatcgagcc ctgccgccgc ctcgcgagcg atccggccca
     2881 cgtcctgcca gatgggtgca gccacgccga tctcccggta ctccccggtg aagaccatgt
     2941 agcgccggat ctcccggacg atctccccct ccttccggta gtactcctgc agatccgggt
     3001 ctttcgtctc ttcgcgggag aggtcgatgt agccggtgag cacgttgagc cgattcaggg
     3061 tgtcgtgccg ggtcacgtcc gagagcaggt tgagtttttt gttcgcaagg cggagcgcct
     3121 cttccgccat ccgggcagcg ctcgtgtcgc ggcccaccga ctggtactcg acgagggagc
     3181 ccgtctcgtc aaagaacgcc atgtgggtcc actgctgcca ccggatctcg ccgtccggca
     3241 tgaccacccg gtgctcgacc gccagtaccg ggtgagcggg ggtgagggag tgaaggctct
     3301 tctggatccg ggcctggtcg tcggccggta tcgcgggagc gtagcgccgc ccgacgaggt
     3361 cgtccggcgg gatgccgatg gagcggcagt aggcgtcgtt tgcgaaggtg atggtgccgt
     3421 cggggagccg gcggtagatc atctcggtct ggttctcgac gatggcgcgg tagcgctctt
     3481 cgctcttcct gagcgcttct tcggcgttgc ggcgatccgt tatgtcccgg atgtagatgg
     3541 agatgccttc acgggtggga acggcgcgca cctcgaaggc gtggtcccga ccgccgacgt
     3601 gaaaccggca ctcgctcatg ccgggccggc cggtctcccg gacttcgcgg aagaacccga
     3661 cgacctcttc agtccgaagg tcggggtaga cgtcgtagat gctcttcccg atgacctcct
     3721 ctccggggtg gcccgagagg ttggccgcca cccggttcca ggatatgatc cggtcctcct
     3781 ggtcgagcgc gaggaaggcg tcgcttatgt tcccggtcag ttcccggtaa cgttcttcgc
     3841 tcctccggcg tgcctcttcg atcatcttct gcccggatac gtcggatagg atccagaggt
     3901 gctcgatcgc cccgtcacgc acgaccgcga cggccgcccc gaccgggagg gtgctgccgt
     3961 cgggccgtgc gagcaggaac tcctgcaccg ggacctcgtc gccccgctcg agcgcggcga
     4021 ccgccgaccg gaaggcggaa agagacccgg ggtggaggtg cgccgggagg gagagacccc
     4081 gcaggcgatc gggggcagga ccgagcagcg cgcccgccgc ccggttcgcc tccaggatca
     4141 ccccgtccgg gccggtgcag aggcaccccg ccgggaggag ggcgaggcgg tgccggcacc
     4201 gccggtaccc ggcgtcgatg ctccggatgc gggcgcccag ggtgtcgatc aggtcgcaaa
     4261 gcccggatcc gggcaggtct cctccagggg ccgcgtcttc gccgaccgcc cggatgcgat
     4321 gatcgagggc ttccaggaga tcgtcgagcg ccggggtggg ggggtgcata cctcacccgt
     4381 tcccccgcac cctgagtgga taaatctatc ccgggtcccc cgtatcctgc ccatcagggg
     4441 cgctcttcgt cgcaggcgcc gttacggccc cggtatgccg gcacccgtca ggggctctcc
     4501 gcctcgagtt cgaccaccag atcctctccc cgccggagcg cctcgatgaa ctcccggggg
     4561 agggtggccg cggtgtgatc ggagcggatg cccacggtcc ggtcggatat aaagtcgctc
     4621 ctccgccaga caagatccgc agggtggtcc agggtgagcg ccgcgctccc ccgtgagcgg
     4681 acctcgacgg tcacgccgcc ggcggtgagg cgggtcgtga ggaccgctct cccgtcgcgg
     4741 agaaccgctt tcaagccggg atcgaggtcg gcggcgcctt tgtcggcccc gatgccgatg
     4801 atgcagtcgc ctgtagcggt cagcgtctcg tcccgcgtca cctcaaacgt cgtcgggtgg
     4861 gctccccgga ccagcgggtg cccccgtgcc cgcacgatat cccttgcctt catgcttaat
     4921 taggtgagga cttctattgc taatgaatga tgagtatcgt ttgccccgcc tgtaaagagg
     4981 agtgcgaaca ccaggttctc cgggaagccg ccgaactggt ggtgcagtgc agtgtctgcg
     5041 gccagatcca ccggatacca aagccccccg aaccccgcat cttcaccgtc aaggcgatcg
     5101 tgagccgcga gacggagtcg caggtctgca gcgtggagat gctcgaagac gaggtcgtga
     5161 gcctcgggga ccatatcgcc gcggagtgcg gggatgaggt ctacggggtt gaggtcaccg
     5221 gcatagaggc cggcgagaag agggtccgtc gggcaaaggc cgaggaggtg acgaccctct
     5281 ggacccgggg catcgagcag gtcgtggtga aggcctccat ccactccgga cgcacgacca
     5341 tccccctcta ccaggccgcg gagggggagg atgagttcgt cgtcggagag gtctacacct
     5401 tcggcgggcg gcggatccgt atatcgcata tcaaactccg cgacggcccg gtcatgcgca
     5461 aagagggctg gaagacggtg gccagccggg tgaaacggat atacggctac ctcgaggggc
     5521 gctacccgcg gacctgagaa ggggcggggg cggcatgctc ctgccgccgg gtatatcgcg
     5581 ctcaatgcgt gggtcgtacc gttgtccgca ccgggtatat cgcgcgtgag gcgtttcacc
     5641 gcatatgatc ggccagctcg cgcgttgccg cgaagggcgc gaagttcggt ggatgggtac
     5701 cggtaactcc ccattcaagg ctccgcgaga ggccgtttca tcgctcatac cctcgagatc
     5761 gcgcgaaggg aggggagggg tagcgatcgc tacgatagaa caggatgagc cggcgcccta
     5821 cccgggcagg ggacggggca ggtgccggcg gatagcggtc tcgaggtctt cgcggtggac
     5881 gagcccctcc atccgctccc tgaccctgtc gtcctcgatg acgagggtcg tgggggtcac
     5941 ccggaggtta tactctttga tatagtgcgg gttcttcacg gcatcgatct cctcgatggt
     6001 gatcccgagg ttctgttcca cctcgtgaag gatcggggcc tgttcttcgc accccataca
     6061 cccctcctgg tagaagttta tcacttttat cgccatatat gggagaaagc agggagacta
     6121 taaaaggctg tggggtaggg gttaccccgt tatgctgttg agggtgatct cggcgctgcc
     6181 gtagcggtat acgatcgtaa gcgagtcagc agtcttttct gtcacttttc cgaaccggag
     6241 cgccggggtg gcggtatcgt tgcccatgga gatatccggc gacgtcgtca gaccgagcgc
     6301 gaccacgtca cccacctgcc gttccgtgaa gttgagcccg ataccatcga catcttcctg
     6361 gctcaggttc atctcgaggt tgttctcccc gtaggagaag ctgacgacct tcacctcccc
     6421 ggggcgcata ccgacgagcc cttcggatat ggcgttggtc tcaaagccga gaaggccgaa
     6481 atccccaaag tccggcgggt actcgaccgg cagggtggcg acgttctcgc ccgagaccag
     6541 accgccgacg ggcatcgtca ggcctctcgt caggaggacg aggttcccct tctcgtattc
     6601 gctcgagaag aggttctggt cggtcgttat gatggggcgc ccgtcctcgc cgcggatggt
     6661 gtagccgatg gccgcgttgt ttcctatctg cgccttctgg ctgctcccga agaccggggc
     6721 gaagtaggtc cccaccatgg ctaccgcaat cagcagggcg atgcccacgt agccccattt
     6781 cagccacgcc ttatcgtcac ccttcggggt tttctgctgt gtctttttca tcagtgtaca
     6841 atcgtctttg ggtgaaataa aatagtaccg tcgtgggcgg ggaggcatcc ggcggggtcg
     6901 cggatacgag aacccggccg cacaccagaa acacgggccg gagagcctct gcccggtgcc
     6961 cggggaagaa ccgcccctca ccagcctctc ttccacgccg gctccggagc ctgcatgccc
     7021 ccttaagagg ggatctgcgc ctggagaagc gtatcgcgca gtcgttcgag cagaacccgc
     7081 gtggctcgct tatcgacatc gccctcgctc atgtaactgc tggtatagat gcaggacggg
     7141 caaccgctct cacaacggca gtccgcgacc agcctgcagg ccatcgcaag gatatccggg
     7201 agcagcccgg caaccttctc cgacaacccg atgcccccga caaagttgtc gtagacgtac
     7261 accgtcggcg ctccggtctc tgcatcccgg tacccggcat ggacgttgat gtcatgcgca
     7321 tcgcacatga tgaagtaggg caggatggtg ggaatgacgt tttcaagccc tctcaggccg
     7381 cgttcaaacc cggcggcccc ggaaaggccc ggctcgttgg gagagaccgg gatcgagaac
     7441 caggtcgccc gggtctcgaa gtggagcggg gggaggtcca gggagatctc gttgatgacc
     7501 ttctcgtgct tgatgacccg gtaaccggtg tagctctcgc tgatgacgac atccccggta
     7561 tggatatcga tggatccggc ccgggtccgg gcaccggggc gggtcacgcg gaggctgacc
     7621 tctcgcgcag cccgggtgta gtaatcggtc tcctcatacc ggacccggat caccctcttt
     7681 tcggggtcaa actcatcgac aatatacgtc tggccgtcat ggaggaagac cgcacccggg
     7741 tgggcttcac ggaatgcctg ggcagggtcg agcgtctcaa gcagtctatg gccgtgcatc
     7801 aacaggaacg agtccgacga tatcgagttc agtctcacgg acccatgagg ccaggtctcg
     7861 ctccccgcgt acgtccagcc ggccccggtc tgccggaggt ctccccgtgc ggccagttcc
     7921 cgaaaggtct ccatgatcgt atccccgaaa gcctctctgt cccggcccgg ttcgaggggg
     7981 agttcctgtg ccgcgcagag gatgtggtcc gcgcggatat accggttctc aagatccagg
     8041 accgcgttct cgtgcggacg ggcaaagaaa tcctccgggt ggcgggtgaa atgctgttcg
     8101 agcgggtttg cgtaggcaac catgatggcg acggactctg tcgcccgcct ccccgccctg
     8161 cccgcctgct gccaggtcga catcatcgtc ccgggatacc ctgagaggat gacgcaatcg
     8221 agcgacccga tatcgatgcc gagttcaagg gcgtttgtcg agacgatacc gaggagcgaa
     8281 ccgtctttta tctgctgctc gatccgcaac cggtcctccg gcaggtagcc gccccggtat
     8341 gacctaaccc gctgtgccag gtgcgggtag tcgtccctga gcgcttcttt cgtccagagc
     8401 gccacaagtt cgaccagctg gcgcgaccgg gcgaagcaga gcgtctgcgt ctccatcctg
     8461 acgcaggccg caagtagttt taccgtctcg ttatgcacgg agagcgcaac cgtatcttcc
     8521 ttcgcaccct ccggtttggg gttgtagagg acgaagtgct ttgagccccg cggggatccg
     8581 tcgcggctga tgtggacgac cggccggccg acgagttttc cgccgaactc ctcggggttt
     8641 gcgatggtgg cggacgagag gatgaactgc ggatccgagc cgtaatgggc gcagatcctc
     8701 tccagcctcc ggaataccag ggcgacattc gacccgaaga ccccgcggta ggtgtgcgct
     8761 tcatcgacga cgatacattt gaggccggca aagaacgacg cccatcttct gtgggttccg
     8821 agcatgtagt gcagttcata ggggtttgtc aggatcacct tcgatgtgca gcggatacgg
     8881 tcgcgctcgt cgccgggtgt atctccatcg aacctctcgg cgttgacgat gatcctggtc
     8941 gccgcctcaa actccttcaa tgttttgtac tggtcgtttg cgagcgcctt gatcgggtat
     9001 atgtagagcg ctgtcgcgcg ggggttgttt aacaggatct cgaagatggg gatgttgaac
     9061 gcgagtgtct tcccgcttgc cgtcggcgtg gtcaggatga tgtggttgcc gccgcggatc
     9121 cgctcgattg cctcgcactg gtgggtatag agccggatcc ccttctgctc caggtatgcc
     9181 gcaagcggtc cggggagagg aggggagact ataccgcagg cgggtttgtg cgagggcagc
     9241 ctctcgatgt gcgcaacctt atctctgcag tgcttatctg tccggagaaa ccggagtatc
     9301 ttcgcggttt ttctggagcg gcgcctcgaa acggggggag ccgcgccaac tcccgcctca
     9361 tcctccttct ccggtgccgc ctcataccgg ggagggagct ccgccacccc gacggcggat
     9421 cccgggttca ggagcgcttt ctcccgcatc cgggcggcct cggcgaacgc cgggtcgatc
     9481 tcgagcgcgc gatcgaagca gcggatcgcc gcatcgacct ccccggcact gagcctcgca
     9541 agcccgaagg cataccaggt atccctctct tcagagacat cgtgagtctt catccccacc
     9601 gcctgatgac gatcacatgg tgcgataaag ggttaatccg ttctgtttcg ccctcacacc
     9661 ggttcattca tcggaataca gcagtatctg ctggtaggag acaccttccg gctccatatc
     9721 cagcccggca tcgaatgcga ccttcggcct gctcctgtag gtgaaggtga gcccgccggt
     9781 gctctggagg tgttcgagga tggtcttcat ctcctcgtag atcgggtacc ggccggtccc
     9841 cgtgagggtc acccatgccc gggaccgggt cagcgcgacg aagaggctgt tcctgagcgg
     9901 gatgctctcg tcgtttctcc cgatgatgtc aaacccgagg atgtagacca cgttcccctc
     9961 gttccccttc gcccggttga tgcccgagac ggtgacgcag cccgtctcgg agaaggtgtc
    10021 cggttttgca ccgatatcaa gccggttccg gcccgaggcc gaggatatgt aggtctcgat
    10081 gccacgctcc atgagttctt ctgccacgct gcgcaccccg gcattcgacc tggcgttgag
    10141 ggagacgacc agaatgtcct cgggacgtag tccgctcacg acgatatcgc gctgtatcga
    10201 acgcgcgaca aacgatgcct cttcctcgcg ccgatcaact atctggaggt cgatgaggtg
    10261 ccctttccag aggtcgggca cgatgtttgc cgtgtactct tccgggcggg tgatcctcac
    10321 ctggcggccg ctcttgaact ccccctctac gtcgtaaccc agcgcgttcc attcggcctt
    10381 tgtggtcggg ccggcgagca tgccctcgcg gaagaagagg cccatgccta tcgcgtgcgc
    10441 cgccatgagg accggagcgg gtgtgcggta gcactttctc atgatgacgc ttttctggag
    10501 tttgaactcc tcgccgaaga tctctcttga tgagggtatt ttgatgctgc tcgtattctg
    10561 gtactcgtcg tatgcccaga tcaggcgcct cttctccggc ctatcggggt ccggcgggca
    10621 gagcgactgg tatgccatcc agaagaacgg ctgctttccc cggtagagga ggtcttcgcg
    10681 gtcgacgacg aggtcctggc cttcgtcgat gaggacggca tcaaagagcg ggtcgagcgg
    10741 gaggtttctg ctctcctgga tctggaggaa actcttgcat gcccgtgcga agagttcgtt
    10801 tggtgtctgg tgccggtccc ccctgaggtc ttcgggggag aagaagtcgg tgtcgtaatg
    10861 gtcgcagagc atccggtaaa agcccgttct gccggcgctt ccccacgcgt gaagtatctt
    10921 gagcgtgctg tgcgggttct ccgggtcgaa ctctccgccg tacatgcggc agtaaaactg
    10981 gacccagtac tcgatgtact cgtagagcga gcgcgtgaag aaggtgtata cgatccggag
    11041 atcgggctgc tcgcagtgca tgtaggccgc tttctggcag aggatatggg tctttcccga
    11101 tccggctatg cccctgaacc tctgcggttc ggagacggcc tcttttgcca gccgttcgac
    11161 ttcgatatcg ccctggacga actgcatcct caacttccgc agtacgtccc tccgcgatgc
    11221 gccgcccggg tctttcacgc tcaccctctc atatcctctc gccgggatga ggcttatgtt
    11281 atctgatcgc tgcccggcaa ccgggttcgc tatgagagcg atagtgcccg gagtataggg
    11341 tgggcggtgc ccggagggtg aatgccaccg tctgtattgc cgggaagggt gcggtggata
    11401 cgggaaagag gcctatggca tcttcacgcg ccccatacag gaggtggagc agatcccgtc
    11461 gcgaccgctt ctgcccgctt gccgtcggcg tcggggggag gggcggttct gcgtggctgt
    11521 atctgcggtg tcgatctcgt gggcactacc gggcagtcac ccgccgggaa ccagcccgct
    11581 cacgcacgcg atatgctccc cggccttcgt gatccggatc gagaagtgcc ggccccgcca
    11641 ctcccgctcc acgtaaccct gctcctcgag ttccctcagg tagccgcggt tcgtccggtt
    11701 caggagcgcc cgcttctttt ttgcccgctg gtagccgtcc cgctcggccg tgtgcagcct
    11761 ctcgtagccg tgggtgtttg cgccgtggaa gaactcgatg atctcctcgc tcgtcatgcc
    11821 gccttcctct ttgcgccgga agagctcgac gaggagcctg acgttctcgg gccgcggcat
    11881 catgatccgg aaaggctgaa agacttctgt cctgacggtc ttgacgatcg agaggccgtg
    11941 ctcgcactct tcaccgtccg ccgtcgcgta gcggtcggcg ctgacatagt agagcatggc
    12001 ctcgtggacc atcgcggcga gcgtcaccgc gacggaggtc ttccggccgc aggaggacat
    12061 gttgacgtaa acggcgttgc cggcctcctt ctcggtgcgg atcaggtaag agacgacctt
    12121 caggacgtcg agtatatcaa acatggcgac cgggacgaac gtcacggcga taccgagatc
    12181 ctgcagctgc tccgtcaccc ggccgacgaa gtagtgctgc tttctgacca tccgggggtc
    12241 gaggccggcg tccggcggga ccgcgaggat gtaggcgcgg tcggccttgt tcttcgtgaa
    12301 cggcgccacc gcccggtcga tctcgtggcc gagggggatg atgtggacga cgtcgcgcag
    12361 gccccccggc aatggatcgt tcttagtggt catgctcatc acaatattca ctgaatacac
    12421 gtgaagttgt taatgtttcg atccggcgca tgctcatctg taggaggtat caaaccatga
    12481 gaaccaacct tcaggggctt gccgggcggc gggggaccgc cgtcttccgg cagtacgggg
    12541 cctgccgggg caggggatgc gccgggcggt ccatcctgct cgggcaggtc cgcgaccttg
    12601 ccgaccgcgc ccggatcaac tacaccgccg ggttcgacgc agtgggagac cttcgccgcg
    12661 gcgatatcgt ccggttcacg gccgcaatcg
//
LOCUS       DAOSII010000051        12690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_4999_length_12690_cov_34.224931, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000051 DAOSII010000000
VERSION     DAOSII010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 12690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12690
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4999_length_12690_cov_34.224931"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..3359
                     /locus_tag="PLV90_05310"
     CDS             216..3359
                     /locus_tag="PLV90_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF96567.1"
                     /translation="MSQPHPPSSGSLAGPSILDALTEGVLLIDREQRVAWINRPLERL
                     LGIDRDPIHGSNAGEFVRRHLGPCIIDRACADRVAASLSMSQSSIEVTCTFRPPGGRV
                     IISDVILQDEPFRGMRLVRFQGIARQKQAEDAFRESDETYRQIFASMGVGFAFYEMVR
                     DSAGRPVDYLILDIDPAYEHIMGAHRDRIVGQRFTEIYPGIEPLWIERYDKVLRTQKP
                     ARFEEYNPKSGRWYDIDAYPMREDDRFAVIFTEITGWKQAEEALQESEERYRTLFESA
                     SDALFIHDRNGKILDVNRAAVERLGYSREELLAICLTDIVLPEFAALVPDQTRAVVER
                     GRMIVETAHLARDGRRIPVETSSQVIPYRKGTAILAVSRDITRRKRSEEVLRHQAEMF
                     ERLIDTIPVMIVIFDPELRQFRFNRAFRETLGWTEEDIAGRDLMTVVYPDPDYRAKAA
                     AFMQSLEPGWREWKVTAKDGSIVESSWANISLADTTRIGIGIDLRERKRAEEALRASE
                     KRFRLLSENASDIVLVFDEQGIISYASPSVRPIGGYAPEDLIGRCIFEFVHPDDLPPA
                     RDALQRAAAHPGRQVTLEVRVRHSGEDWLYLDLTVNNLLDESAIRGYVVNARDITRRK
                     LAEEEVLQSERSLAAELDAARHLQQVSTQLIQADRVEALYEQILDTAVTILAADFASI
                     QMLDVNRGPTGELQLLGHRGFTEEAARFWEWVCPGSESACGKALSTRQRVIIPDIRDC
                     EFLAGSADRDILMQTGIRAVQTTPLYSRSGTLLGMLSTHWRRPHEPTASELRAIDLLA
                     RQAADLIDRRRAEDALQCRTEDLIRKSEELEAARDTANIYLDILTHDVRNANNVSGMY
                     ADLLVGMTEGDLKTYAEKLHASIGRSTEILQNVATIRRAQEEAGLVPVNLDAVIREEI
                     ATFRGATIRYAGHEVNVRADGLLPTVFTNLIGNAIKHGGPGVVVTVRVEERDGEVLVS
                     VEDTGQGVPDAVKKRLFTRFERGMARGSGQGLGLFIVRTLVARYGGRVSIEDRVPGHP
                     EEGAAFRFTLKRVAQDG"
     gene            3716..6430
                     /gene="cas3"
                     /locus_tag="PLV90_05315"
     CDS             3716..6430
                     /gene="cas3"
                     /locus_tag="PLV90_05315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HOF96568.1"
                     /translation="MSSPAPDGIYRYWGKYNTDDGLTWHLLAYHCLDVAAVGRELIRR
                     DPQMFQKIAGTIPLRDEQICSLITFFLAVHDIGKFSGRFQGINPALQSHLQGTACRMP
                     YSHHHTAMGMLAFMHEVWPLALAEGWFGTDPGEDPYDLEDLFLPWIRASAGHHGKPVT
                     GNGTFSELFGPMDREAVCEFARACAGLFLSGPPEESIRYSTELHETFTRVSWLIAGLT
                     VLSDWIGSDAGIFRYHQDTIPLSEYWQRCAVPGAKATVDAAGVLPSVIAPFPGMASLL
                     PENAVPTPLQETVAGYELAGGPSLFIIEDVTGSGKTEAALAMAHRLMEGGSAEGIFMG
                     LPTMATANGMYRRFGEKYRQLYRDGEHPSLLLAHSARELSSLWRQSIGPKIDKKTFSP
                     DGEAGSAECAAWLSDNRKKALLADVGVGTVDQALMSVLPLHHQSLRLLGLARHVLIVD
                     EVHAYDEYMNTLLERLLQFHAAFGSSAILLSATLPGRLRERFVAAYCSGLRIPAPPIR
                     EADPPLVTVVSTGGVDEKALQANALSRRTVAAELTDNEADVESYLEHALQEGRCACWI
                     RNTVTDAIETYQRLAGRWGDEHVKLFHARFTVGDRQRIEDDVLRWFGKRSTDADRRGK
                     ILIATQVVEQSLDIDFDAMVTDLAPIDLIIQRAGRLHRHRERRGRTDVIPVLLILSPT
                     PVKAPGRDWFTSVFPRGGRVYEKHGQLWLTARLLAARGRIVMPEDARLLIEGVFGETA
                     QASVPDALKYWEIQADGKDRGHAALAQINALRPETGYADLHGQWEDDSRTPTRLGEPS
                     ITVVLARRERDGFRPWSDGGEDAWARSQVSVRESLIAGPVYSSPADESEAERVKPFLP
                     GRGKGSVLIPLTLAADGSGEGRARNGKENEVTVTYDPRIGLQIREGRL"
     gene            6641..8269
                     /gene="casA"
                     /locus_tag="PLV90_05320"
     CDS             6641..8269
                     /gene="casA"
                     /locus_tag="PLV90_05320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HOF96569.1"
                     /translation="MVNLIDDPWIPVVRRDGTRETIAPWEITGGTEPAIRLDAPRPDF
                     NGALIQFLIGLAQTAIPPGDNRDWRRRFKTPPPPDELKAAFAPYVHAFNVDGKGPLFM
                     QDYDLSEGAENPVDRLLMEMPGEQTLKLNTDHFLKRGQVKRICRICCTMALFALQTNA
                     PSGGRGHRTSLRGGGPLTTLVTGTTLWETVWLNVLPRDVFEILGNTARTADSDRFPWM
                     GPTRTSTKNEVTAPTDVHPAQIFFSMPRRIRIDLDRPASGECDICGCASDALISRYIT
                     KDGGVNYKGAWKHPLTPYYQPKNAEEPLPKHGQADGISYRNWLGFVQNDPESRTQPAL
                     TVQVFRNERQRYLTDYLGRSAPLWAFGYKTDNMKAVCWNESTMPLIHVDDGIRERYEE
                     GVAALIKTAGLVAASTRSCIKKALFGDRKDAGGNIPEVDSRFWQETESEFYITLEALR
                     EAMERGEDMTPLKLQWLSSLSGEATKLFEAYSQADLIGVADPRRIALSRRMLRNYISP
                     RNKKIRDALDLPKDPDDGEAKPGKRPAKARGEHL"
     gene            8266..8772
                     /gene="casB"
                     /locus_tag="PLV90_05325"
     CDS             8266..8772
                     /gene="casB"
                     /locus_tag="PLV90_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HOF96570.1"
                     /translation="MKQMNYISFTNNPDARSALVEWWGGLERARGDRAALRRCRSAQE
                     IAFVPAFHRLRHDLSRIAPVDAEKLAVVAGILSHVRKNDYSLRFAQQMATSKDGGDRA
                     RVSGLRFRRLLKIEGRDDLYGAIVRIVRLLDGSVNIASLADGVYWWNERTKNNWAFDY
                     YDRAPEES"
     gene            8794..9897
                     /gene="cas7e"
                     /locus_tag="PLV90_05330"
     CDS             8794..9897
                     /gene="cas7e"
                     /locus_tag="PLV90_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HOF96571.1"
                     /translation="MSEFIQLHMLVSYPPSNLNRDDLGRPKTAMMGGAQRLRVSSQSL
                     KRAWRTSDIFAGALAGHLGIRTKEMGTLVYRSLASGVSLLEIVQGTRDAPATFKPVPE
                     EKARKWASGIAGIFGKLKPGDDALEIEQLAHFSPEEIAAIDALVAKLAATGKDPGEDD
                     KKLLMERHTAADIAMFGRMLAASPIYNTEAAVQVSHAVTVHTVAVEDDYFTAVDDLNR
                     GEEDLGAGHLGETEFASGLFYLYVCVNRDLLRDNLGGDEALTAASLRALVEAATKVAP
                     TGKQNSFASRAYASYLLAERGCQQPRSLSVAYLKPLQDADLLGSAIRVLKETRDKMDA
                     VYGKCSDACCDMNAHAGEGTLQEVLDFVAGHHA"
     gene            9890..10624
                     /gene="cas5e"
                     /locus_tag="PLV90_05335"
     CDS             9890..10624
                     /gene="cas5e"
                     /locus_tag="PLV90_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HOF96572.1"
                     /translation="MREFLIFRLYGMMASWGDIAVGELRPTFDHPSRSAIVGLLAAAL
                     GIRRDEEEQLSALSNACRIAIRVDAPGVLLRDYHTSQVPPAGRGRKKRSFATRKEELA
                     IPREDLSTVLSTRDYRCDAVSTVCVESAPCSLADLANALNNPVFVLYLGRKSCPPALP
                     VYAQVVRGENLAAAFSSARFPDGDLLQGLLGDGDLRVFWEGDEDAGIPAIQTTLRYDN
                     PLSRKRWQFGSRAEHYGVLRGGSSCT"
     gene            10615..11301
                     /gene="cas6e"
                     /locus_tag="PLV90_05340"
     CDS             10615..11301
                     /gene="cas6e"
                     /locus_tag="PLV90_05340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HOF96573.1"
                     /translation="MYLSRIRLRPDAAGKREFWQAMGSEYQAHHMVWDLFTDSPERER
                     DFLYRVEDVDGLPTIYATCRREPVNRGGMWTIETKPYDPILRSGQQLAFVLRANPVRT
                     KCDEKGKHHRHDVVMEAKTRLKQQGRSREEWPPEPEIVGQAGFAWLATRGEANGFSVA
                     EGDVRADGYTQRRFRKRKGRHDISLSTIDYTGILTVTDPDLFKVALFGGIGPAKGFGC
                     GLMMVRPAGR"
     gene            11335..12225
                     /gene="cas1e"
                     /locus_tag="PLV90_05345"
     CDS             11335..12225
                     /gene="cas1e"
                     /locus_tag="PLV90_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HOF96574.1"
                     /translation="MAVKERVSLLFLEKGELDVLDGAFVLVDKNGVRTQIPVGSVACL
                     MLEPGTRVSHAAVVLAARVGCLLVWVGEAGVRLYAAGQPGGARSDRLLYQAKLALDEE
                     ARLKVVRKMYEIRFQGPVPSQYSVMQLRGMEGARVRELYVQLARKYGIEWKGRDYDPA
                     SWGSSDLSNRCLSSATACLYGICEAAVLAAGYAPAIGFIHSGKPLSFVYDVADLFKFD
                     TVVPVAFRVAAENSFDPERAVRLACRDSFRETRLLKKIIPTIEEVLKAGGLPMPEPPG
                     DALRPAIPEERGLGDAGHRG"
     gene            12206..12565
                     /gene="cas2e"
                     /locus_tag="PLV90_05350"
     CDS             12206..12565
                     /gene="cas2e"
                     /locus_tag="PLV90_05350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HOF96575.1"
                     /translation="MLVIVVEAAPPRLRGRLAVWLLEVHAGVYVGDLSVKVREMIWQQ
                     VVAGIEGGSAVMVWSTNTEQGYDFTTLGTNRRVPVEFDGVRLVSFLPVPGRDSAGVIA
                     GDDKEDGIECGSFDSPE"
BASE COUNT         2461 a         3853 c         4161 g         2215 t
ORIGIN      
        1 cgggtgagcg gtccgcaacg agggggcccc ggggtgagag agaagcaaac tcttttctac
       61 ccccgtgaac aaaggatcac tgatgcccga ccgatcccgg gaccagcgtg gtcccgggca
      121 gatcgaacct ttactctcac cgcttcatcc cgtaaataat tttatgcgtt aatatttata
      181 gaaattgagt ttgaacttgc aggagtaccg acaacgtgag tcagccacac cccccttcat
      241 ccgggtcgct cgcaggccca tccattctcg atgccctcac cgagggcgta ctcctgatcg
      301 accgggagca gcgggtggct tggatcaacc gcccgctcga gcgccttctc ggcatcgatc
      361 gggaccccat ccacggcagc aacgcaggag aattcgtgcg ccggcacctc gggccgtgca
      421 tcatcgacag ggcgtgtgcg gatcgggtag cggcgtcact ctcgatgtcg cagagcagca
      481 tcgaggtcac ctgcacgttc cggccacccg gcggccgggt catcatatcg gatgtcatcc
      541 tgcaggacga accgttccgg ggcatgcgcc tcgtccggtt ccagggtatc gccaggcaga
      601 aacaggcgga ggatgccttc cgggagagcg atgagacgta tcggcagatc ttcgcgtcga
      661 tgggggtggg tttcgcgttc tacgagatgg tccgcgacag cgccgggcga cccgtcgact
      721 acctcatcct cgacatcgat cctgcctatg agcatatcat gggggcgcac cgcgaccgga
      781 tcgtgggcca aaggttcacc gagatctatc ccggtatcga accgctctgg atagaacgct
      841 acgataaggt tcttcgcacg caaaaaccgg cgcggttcga ggagtacaac ccgaagtccg
      901 gccggtggta cgacatcgac gcctacccga tgcgggaaga cgaccggttc gcggtcatct
      961 tcaccgagat caccggatgg aagcaggcag aagaggcgtt gcaggagagc gaagagaggt
     1021 atcgtaccct ctttgagagt gcaagcgacg cgctcttcat ccacgatcgc aacgggaaga
     1081 tcctcgacgt aaaccgggca gcggtcgagc gccttggcta ctcacgggaa gaactgctgg
     1141 caatatgcct caccgacatc gtcctgccgg agtttgccgc tctcgtgccc gaccagaccc
     1201 gggcggtcgt ggagcgcggc aggatgatcg ttgagaccgc ccatctcgcc cgcgacggga
     1261 ggcgcatccc cgtcgagacc tcatcacagg tcatcccgta ccggaagggg acggctatcc
     1321 ttgccgtctc ccgggacatc acccggcgaa agcgctcgga agaggttctg cgccatcagg
     1381 cagagatgtt tgagcggctc atcgatacga taccggtcat gatcgtcata ttcgacccgg
     1441 aactgcggca gttccggttc aaccgggcgt tccgcgagac cctgggctgg accgaggagg
     1501 atattgccgg ccgtgatctc atgaccgtgg tctatcccga cccggactac cgggcaaaag
     1561 ccgcggcgtt catgcagtcg cttgaaccgg gctggcggga gtggaaggtg acggcaaaag
     1621 acggcagcat cgtcgagagt tcctgggcaa acatcagcct tgcggacacg acccggatcg
     1681 ggatcggcat cgacctccgg gagcggaaac gtgcggagga agccctgcgg gcgagcgaga
     1741 agcggttccg gctgctctcc gagaacgctt cagacatcgt cctcgtcttt gatgagcaag
     1801 ggatcatatc gtatgcgagc ccctcggtga gaccgatcgg cgggtatgcg cccgaagacc
     1861 tcatcgggag gtgcatcttc gagtttgtgc atcccgatga cctgccgccg gcccgggatg
     1921 ccctgcagcg tgccgccgcc cacccgggca ggcaggtcac cctcgaagtc agggttcggc
     1981 acagcgggga ggactggctg tatctcgacc tgaccgtcaa caacctcctg gatgagtcgg
     2041 ccatccgggg ctacgtagtg aacgcccgcg acatcaccag gcggaagttg gcggaagaag
     2101 aggtactgca gagcgaacgg tccctcgcgg cggagctgga cgctgcacgc cacctccagc
     2161 aggtcagtac gcagttgatc caggccgaca gggtcgaggc gctctacgag cagatcctcg
     2221 ataccgcggt gacgatcctc gccgccgact tcgcgagcat ccagatgctc gatgtaaacc
     2281 gcgggcccac gggagaactc cagctcctcg gccaccgcgg gttcaccgaa gaggccgcac
     2341 ggttctggga gtgggtgtgc cccggttccg agagcgcctg cggaaaagcg ctctccacca
     2401 ggcagcgcgt tattataccg gatatccggg actgcgagtt cctggccggc agcgccgacc
     2461 gggacatact catgcagacc ggcatccgcg ccgtgcagac cacgccgctc tactcgcgtt
     2521 ccggcaccct gctcggcatg ctctccacgc actggcgccg gccgcacgag cccacggcaa
     2581 gcgaactgcg tgcaatcgac ctcctggcgc gccaggcggc ggatctcatc gaccgcaggc
     2641 gggcagagga tgctctccag tgccgcaccg aagacctcat ccggaagagc gaagaactcg
     2701 aagctgcccg cgataccgcc aacatctacc tcgacatact gacccacgac gtccggaacg
     2761 caaacaacgt ctcgggcatg tacgccgacc tcctggttgg tatgacggaa ggcgacctga
     2821 agacctacgc agagaagttg catgccagca tcggccggag caccgagatc ctccagaacg
     2881 tcgccaccat ccggcgggcc caggaggagg cggggcttgt gccggtgaac ctcgatgcgg
     2941 tcatcaggga ggagatcgcc acttttcgtg gcgcaaccat ccggtatgcc ggtcatgagg
     3001 tcaacgtgcg ggcggatggc ctcctgccga ccgtctttac aaacctcatc gggaacgcca
     3061 tcaaacatgg cggccccggt gtcgtcgtca ccgtccgggt cgaggagcgg gacggcgagg
     3121 tgctggtctc ggtcgaggat accggccagg gggtgccgga tgcggtgaag aagaggcttt
     3181 tcacgcggtt tgaacggggc atggcccggg ggagcggtca gggtctcggc ctcttcatcg
     3241 tccggacgct tgtcgcgcgg tacgggggcc gggtctctat cgaagacagg gtgccgggcc
     3301 atccagagga aggggcggcc ttccggttta cactgaagag agtcgcgcag gacgggtgaa
     3361 cccgggcgca cggttcgggg gcgggccgat gcagccggcg gggaggggca cggaaggggt
     3421 gtttgccggg tatcgggaga tgcctgcctc cggcgttgca gctcaaagaa acgccgggcg
     3481 caggagcccc acctcacggg ttttgcagcg ctgcatgggc tgcgtagatt ctggtaagat
     3541 ttcgcgtgaa ggagtgcgaa acaagaaatg gaatggatcg ttaccacgag ggcgacagcc
     3601 tggcgaggaa aacggatacc cccctcccga gctgccgcaa cagggcaaca agaagaacga
     3661 accgggtttt ccagaccagc aggcctatca tatccaaccg gcatatgaac ttctcatgag
     3721 ctcgcccgcg cccgatggca tctaccgcta ctggggaaag tacaacaccg atgacggcct
     3781 tacatggcac cttctcgcct accattgcct ggacgtcgcg gcagtcggtc gcgaactgat
     3841 acggagagac ccacagatgt tccaaaagat cgccgggacg atccccctca gggatgagca
     3901 gatctgctcc ctcatcacgt ttttcctcgc cgtccacgat atcgggaagt tctccgggag
     3961 gttccagggt atcaaccccg cccttcagtc ccacctccag ggcaccgcct gccggatgcc
     4021 atacagccac caccacacgg caatgggcat gctcgccttt atgcatgagg tatggccgct
     4081 ggcgcttgcc gaagggtggt ttggaacgga ccccggggaa gatccgtacg atctcgagga
     4141 cctcttcctc ccgtggatac gggcatctgc cggccaccac ggaaaacctg tcaccggcaa
     4201 cggcacgttc tccgaactct tcggcccgat ggaccgggag gcggtctgcg agttcgcccg
     4261 ggcctgcgcg ggtctcttcc tctcggggcc tcccgaagaa tccatccggt attcaaccga
     4321 actgcacgaa acattcaccc gcgtatcgtg gctgatcgcc gggttgaccg ttctgagcga
     4381 ctggataggg tcggatgcgg ggatattccg gtatcatcag gatacgatac ccctttcgga
     4441 gtactggcag cgctgcgccg tcccgggagc gaaggcaacg gtcgacgcag caggcgtcct
     4501 gccatccgtc atcgctcctt ttccggggat ggcgtccctt ttgccggaaa acgctgtccc
     4561 gacacccctg caggagaccg tcgccggcta cgaactggcc gggggaccct ccctcttcat
     4621 catcgaggac gttaccggga gcgggaagac cgaggccgca cttgctatgg cacatcgcct
     4681 catggagggc gggtctgccg aaggcatctt catggggctg ccgacgatgg caaccgcgaa
     4741 cggcatgtat cgccggttcg gtgaaaaata ccggcagtta taccgggatg gcgagcaccc
     4801 ctcgctcctg ctggcccaca gtgcgcggga gctctccagc ctctggcgcc agtccatcgg
     4861 cccgaagatc gacaaaaaga ccttctcccc cgatggagag gcggggtcgg ccgaatgtgc
     4921 agcctggctc tcggataacc ggaagaaagc cctcctcgct gatgtcggcg tcggcaccgt
     4981 agaccaggcg ctcatgtccg tgctgccgct ccaccaccaa tcgctccgcc tgctcggcct
     5041 tgcccggcac gtgctcatcg tcgacgaggt gcatgcctac gacgagtaca tgaacaccct
     5101 tcttgagcgg ttgctccagt tccacgcggc ttttggcagc agcgcgatct tgctctcggc
     5161 gacgcttccc ggcaggctcc gggagcggtt cgttgccgct tactgctccg gcctccggat
     5221 accggcaccg ccgatacggg aagccgatcc tcccctggtg accgtcgtct ccacgggagg
     5281 tgtggatgag aaggcgctgc aggcaaacgc tctctcccgg aggaccgttg cagccgaact
     5341 caccgataat gaggcagatg tggaatctta ccttgagcat gccctccagg aaggccggtg
     5401 cgcctgctgg atccggaata ccgtcaccga tgcgatcgag acctaccagc gtcttgccgg
     5461 gcggtggggt gacgaacacg tcaaactctt ccacgcccgg ttcaccgtgg gggaccgcca
     5521 gcggatcgag gacgatgtgc tgagatggtt cgggaagcgg agcacggacg ccgaccgcag
     5581 ggggaagata ctgatagcga cccaggtcgt cgagcagtcg ctggatatcg atttcgatgc
     5641 gatggttacg gatcttgcgc ccatcgacct gatcatccag cgggccggaa ggctgcaccg
     5701 gcaccgggag agaaggggca ggaccgacgt aatacccgtc cttctcatcc tctctcccac
     5761 tcccgtcaag gccccggggc gagactggtt tacgagcgta ttcccgaggg gcggccgggt
     5821 atacgagaag cacggacaac tctggcttac cgcacgcctg cttgccgccc gggggcggat
     5881 cgtgatgccc gaagatgccc ggttgctgat cgagggcgtc tttggagaga ctgcacaggc
     5941 atccgttccc gatgcactca agtactggga gatacaggct gacggaaaag accgtgggca
     6001 cgcggcgctg gcacagataa acgcgttacg gccggagaca gggtacgcag acctgcacgg
     6061 ccagtgggag gacgacagtc ggacaccgac ccggctcggc gaaccgagca tcaccgtcgt
     6121 gctggctcgc cgggagagag acgggttccg gccctggagc gacggtggag aagatgcctg
     6181 ggcgagaagc caggtctctg tccgggagtc cctgatagcg ggcccggtgt actcttctcc
     6241 ggccgacgag agcgaggccg agcgggtaaa accgttcctg cccgggaggg gaaaaggcag
     6301 cgttctcatc cccctgaccc tggccgctga tgggtcgggg gaggggcgcg cacgaaacgg
     6361 gaaggagaac gaggtgaccg tcacctacga tccgaggatc ggtctgcaga tccgggaagg
     6421 gagattgtaa tatccatcta cacggccctg atacctcatt tggcgcaaat tttttcctat
     6481 tatcatcgga gtcctcctca gaagcgcctg atgaaaagat ataagatatt ttataatgaa
     6541 tggctcattg tcattgtctc acctgtcttt tccggtcgca tgagaggtcc ttcaccccgc
     6601 tggccggcac cagacggccg gacaatgcag gtgaagagac atggtcaatc tcatcgacga
     6661 tccatggata cccgtcgtgc ggagggatgg aacgcgggaa accatcgccc cctgggagat
     6721 caccggggga accgagcccg ctatccggct ggatgcaccc cgacccgatt tcaacggggc
     6781 gctcatccag tttctcatcg gccttgcaca gacggcgatt cctcccggcg acaaccggga
     6841 ctggaggcga aggttcaaga caccaccgcc tcctgatgaa ctgaaggccg cttttgcacc
     6901 gtacgtccac gctttcaacg ttgatggcaa aggcccgctg ttcatgcagg actacgacct
     6961 cagtgagggt gccgagaacc ccgtggaccg gctgctcatg gagatgcccg gggagcagac
     7021 gttgaaacta aacactgatc acttcctgaa gcgagggcag gtcaagcgca tctgccggat
     7081 ctgctgcacg atggccctct ttgcgctcca gacgaatgct ccgtccggcg gccgggggca
     7141 ccggacgtcg ctccggggag gggggccgct taccacgctg gtcacgggca cgaccctgtg
     7201 ggagaccgta tggctgaacg ttcttccccg ggatgtcttc gagatcctcg ggaacaccgc
     7261 gagaaccgca gactccgacc ggttcccctg gatggggccg acccgcacga gcactaaaaa
     7321 tgaggtcaca gcccctaccg acgttcaccc ggcacagatc ttcttctcca tgccccgcag
     7381 gatccggatc gacctggacc gccccgcatc gggagaatgc gatatctgcg gttgcgcatc
     7441 ggatgctctc atatcgcggt acatcacgaa ggatgggggc gtgaactaca aaggggcctg
     7501 gaaacacccg ctcacgccat attaccagcc aaaaaacgcc gaagagcccc tcccgaagca
     7561 cgggcaggcg gacgggatat cgtaccggaa ctggctcggg ttcgtgcaga acgatcctga
     7621 gagccgtaca cagccggctc ttacggttca ggtcttccgg aacgagcggc agcgttacct
     7681 cacggattat ctgggccgtt cagccccgct ctgggcgttt ggctacaaga cggacaacat
     7741 gaaggcggta tgctggaacg agagcacgat gccgctgatc cacgtcgatg acgggatccg
     7801 cgagcgctat gaggaggggg tggcagcact gatcaagacc gccggcctcg ttgcggcgag
     7861 cacccgcagt tgcatcaaga aagcgctctt cggggaccgg aaggatgcgg gcgggaacat
     7921 ccccgaggtt gactcgcggt tctggcagga gaccgagagc gaattctaca tcacgctgga
     7981 agcgttgcgg gaggcgatgg agaggggcga ggatatgacc cccctgaaac tgcaatggct
     8041 ttcaagcctc tccggtgagg ccacgaaact cttcgaagcc tattcccagg ccgatctcat
     8101 cggcgtcgca gacccgcgaa ggatagccct gtcccggcgc atgctcagga actacatctc
     8161 gcccaggaat aaaaagatcc gcgatgcgct cgatctcccg aaagatcctg atgacggcga
     8221 ggcgaaaccg ggaaaacgcc cggcaaaagc acgaggtgaa catctatgaa acagatgaac
     8281 tatatttcgt ttacgaacaa cccggacgcg agatcggccc tggtcgaatg gtgggggggc
     8341 ctggaacgcg cccgtggaga ccgggcggcc ctccggcgtt gccggagcgc ccaggagata
     8401 gcgtttgtcc cggcgtttca ccggctgcgc catgacctct cccggatcgc ccccgtggat
     8461 gccgagaaac ttgcggtggt ggccgggatc ctttcgcacg tcaggaagaa cgactactcg
     8521 ctccggttcg cgcagcagat ggcgacatcg aaggacggtg gcgaccgtgc ccgggtgagc
     8581 gggttgcgct tccggcggct gctcaagatc gaggggcgcg acgacctcta cggtgcgata
     8641 gtccggatag tccggctgct cgacggctcc gtgaacatcg caagccttgc ggatggagtc
     8701 tactggtgga atgagaggac gaagaacaac tgggcttttg actactatga cagagccccg
     8761 gaagaatcgt gatcatcaga aggaggagtt aacatgagtg aatttatcca attgcacatg
     8821 ctcgtatcgt acccaccgtc aaacctgaac cgcgacgacc tcggcagacc caagaccgcc
     8881 atgatgggcg gcgctcagcg cctgcgcgtc tcgtcgcaga gcctgaagcg ggcctggcga
     8941 acgtcggata tatttgccgg tgcgcttgcc gggcaccttg gtatccggac aaaggagatg
     9001 gggactctgg tctaccgctc cctggcatcg ggggtcagcc ttctggagat cgtgcagggc
     9061 acgagagatg cgcctgcaac gttcaaaccg gttcccgagg agaaagcccg gaaatgggca
     9121 tcagggatcg ccggcatctt cggaaaactg aagcctggcg acgatgccct cgagatcgag
     9181 cagctcgccc acttcagccc cgaggagatc gctgctatcg acgcactggt cgcaaaactg
     9241 gcggccaccg ggaaagatcc cggcgaggac gataaaaaac tcctgatgga gcggcatacc
     9301 gcggccgata tcgccatgtt cggccgcatg ctggcggcat ccccgatcta caacacggag
     9361 gcggcggtgc aggtctctca cgcggtcacc gtgcataccg tcgcggtcga ggacgactac
     9421 ttcacggcgg tcgacgacct caaccggggc gaagaagatc tgggggccgg gcatctcggg
     9481 gagacggagt ttgcatccgg gctattttat ctgtatgtct gtgtcaaccg agacctcctg
     9541 cgcgataacc tcggcggcga cgaagcgctc accgcggcct ctctccgcgc cctcgtcgag
     9601 gcggcgacga aggttgcgcc gaccggcaag cagaacagtt tcgcgtcgcg ggcatacgcg
     9661 tcgtacctgc ttgcggagag agggtgccag cagccccgct cgctctcggt tgcatactta
     9721 aagccgctgc aggatgccga cctgctcggg agcgccatca gggtcctgaa ggagacccgc
     9781 gataagatgg atgccgtgta cgggaagtgc agcgacgcct gctgcgatat gaacgctcac
     9841 gccggcgagg ggacgttgca agaggttctg gatttcgtgg cgggtcacca tgcgtgagtt
     9901 cctcatcttt cgcctctacg gcatgatggc atcgtggggc gatatagcgg tcggggagct
     9961 ccgcccgacc tttgaccacc cgtcccgatc cgctatcgtc gggctgctcg cggcggcgct
    10021 cgggatccgc cgcgacgagg aggagcagct ctccgctctc tcgaacgcct gccggatcgc
    10081 gatccgggtg gatgcgcccg gcgtgctgct gcgggactac cacacctcgc aggttccgcc
    10141 cgccgggaga ggacgaaaga aacgctcgtt tgccacccgg aaggaggagc tcgcgatacc
    10201 ccgcgaggac ctctcgacgg tgctctccac ccgggattac cggtgcgacg ccgtctcgac
    10261 ggtatgtgtt gaatcggcgc cctgctcgct ggcggacctg gcaaacgccc tgaacaaccc
    10321 ggtcttcgtc ctgtatctcg ggaggaagtc ctgcccgccc gctctcccgg tttatgcgca
    10381 ggttgttcgc ggcgagaacc ttgcggccgc gttctcgtcg gcgcggttcc ccgacggcga
    10441 ccttttgcag gggttgctcg gcgatggcga cctgcgggtc ttctgggagg gagatgaaga
    10501 tgcagggatc ccggcgatcc agacgaccct gcgttacgac aatccgttga gccggaagcg
    10561 gtggcagttc gggagcaggg ccgaacatta cggtgttttg cggggcggat cgtcatgtac
    10621 ctgagcagga tacggctccg gccggatgcg gccgggaagc gtgagttctg gcaggcgatg
    10681 gggagcgagt accaggcgca ccatatggtc tgggacctct tcaccgacag tcccgagagg
    10741 gagcgggact ttctctaccg ggtggaggac gtcgacggcc tgcccacgat ctacgcgacc
    10801 tgccggcgtg agccggtgaa ccggggcggt atgtggacga tcgagacgaa accctacgat
    10861 cccatcctcc gctcagggca gcaactcgcg ttcgttctcc gtgccaatcc cgtccggacg
    10921 aagtgcgacg agaaggggaa gcaccaccgc cacgacgtgg tgatggaggc aaaaacccgg
    10981 ttaaagcagc agggaagatc cagggaggag tggccgccgg agccggagat cgtcgggcag
    11041 gcgggattcg cgtggcttgc gacgagaggc gaggcgaacg gtttctcggt ggcggagggc
    11101 gacgtccgcg ctgacggcta tacccagcgg cggttccgga agcgcaaggg ccggcacgat
    11161 atcagtctca gcacgatcga ctatacgggc atcctcacgg ttaccgatcc ggacctcttc
    11221 aaggtggccc tgtttggcgg gatagggccg gcaaaagggt tcgggtgcgg gttgatgatg
    11281 gtgcggccgg cagggcggta ggggggagga cgtacgcttc ctcccctcaa gccgatcgcc
    11341 gtcaaagaac gggtttctct cctgtttctt gagaaaggcg agctggacgt gctcgacggc
    11401 gcttttgttc tggtggacaa aaacggggtg cgcacccaga tcccggtggg gagcgtcgcc
    11461 tgcctgatgc tcgagcccgg gacccgggtc tcgcacgcgg cggtggtgct cgccgcccgg
    11521 gtcggctgtc tgctggtctg ggtcggggag gcgggggtgc gtctctacgc tgccgggcaa
    11581 cccgggggcg cgaggtcgga tcgcctgctc taccaggcga agcttgcgct cgacgaggag
    11641 gcccggctga aggtggtgcg gaagatgtac gagatccggt tccagggccc cgtcccgtcg
    11701 cagtattctg ttatgcagtt gcgcgggatg gagggcgccc gggttcggga gctctacgtg
    11761 cagctcgcga ggaagtacgg catcgagtgg aaggggcggg attacgatcc cgccagctgg
    11821 gggagcagcg atctctcgaa tcgctgcctg agttctgcga cggcctgtct ctacgggatc
    11881 tgcgaggcgg cggtgctcgc cgccgggtat gcacctgcta tcgggtttat ccattcggga
    11941 aaaccgctct cattcgtcta cgacgttgcc gatctcttca agttcgatac ggtggtcccg
    12001 gttgcgttcc gggtggctgc agagaactcg ttcgacccgg agcgggcggt gcggctggcg
    12061 tgccgggaca gtttccggga gacgaggctg ctcaagaaga tcatccccac catagaggag
    12121 gtcctgaagg cgggcggact tcccatgccc gagccgcccg gggatgcgtt gaggccggcg
    12181 ataccggagg agcgggggtt gggcgatgct ggtcatcgtg gttgaggccg ccccgccccg
    12241 gctccggggc cggctcgcgg tctggctgct cgaggttcac gccggggtgt acgtcgggga
    12301 cctctcggtg aaggtcaggg agatgatctg gcagcaggtc gtcgccggga tcgagggagg
    12361 cagcgccgtg atggtctgga gcaccaacac cgagcagggc tacgacttca cgacactcgg
    12421 gacaaaccgc cgcgttccgg tggagttcga tggcgtgagg ctggtgtcgt tcctccccgt
    12481 gccagggaga gattcagctg gtgttattgc cggagacgac aaagaggacg gtatcgaatg
    12541 tggttcgttc gacagtccgg agtgaggcag atgagggagc cggctccccg tgacgatctc
    12601 ctgatcctct gcttgggccg gtggaagtaa ccttccacct ttctggaata aaaagggatg
    12661 agcgttcccc atgtacgtgg ggatgaaccg
//
LOCUS       DAOSII010000052        12668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_5008_length_12668_cov_53.521922, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000052 DAOSII010000000
VERSION     DAOSII010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 12668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12668
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5008_length_12668_cov_53.521922"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="PLV90_05355"
     CDS             <1..633
                     /locus_tag="PLV90_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HOF96576.1"
                     /translation="QANNGDALAVCRRVRGSLATFHDLVLASVIGNTLDYGSKAHTVT
                     DDFVGFFHREFASGLTVDDTDRMEPLTARVVYLADNCGEIVFDALLAEHLRKSGSHVT
                     FAVRGAPILNDATIEDALALGLDRRVDILTTTTDGIAELGLNRDLLPPILADALDRAT
                     LVIAKGMANYESLSDEGDLPPVAYLMSVKCGPIGADIGIPVGSRVALLRE"
     gene            complement(1159..2373)
                     /locus_tag="PLV90_05360"
     CDS             complement(1159..2373)
                     /locus_tag="PLV90_05360"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOF96577.1"
                     /translation="MDIGTLADAGKLKGTVLLRVDLNSPIDPSSNQILDDKRFREHLP
                     TVQALEDARLVILTHQSRPGKKDYTTLEAHAAKFERLLGRPVTYVDDVFGRCARDAIR
                     GAKRGDILMLENLRFAAEENLTLKPEEAKKTILVRRLAAMADFYVNDAFGTAHRSQPS
                     IVGLPLLLPSVAGLLMEKEVANLSRVFTGAPRPVTFVLGGTKVDDSIAVAENVLLRGT
                     ADRVIVVGVVGNVFLLAAGYDIGRPSARLIDDLGYRGEIERARDLLKTYRDRILMPHS
                     VAVREDGGRAEYPVDAVPEDAQVLDIGSESIDRLTEAVSASGTVVVNGPAGLFEEEQF
                     AVGTFDLLRAASTVKFSVVGGGHSGAAIERLGIEDRFTHISTGGGAAIEFLTGKKMPA
                     LEALEMSKKIFG"
     gene            complement(2395..3021)
                     /locus_tag="PLV90_05365"
     CDS             complement(2395..3021)
                     /locus_tag="PLV90_05365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOF96578.1"
                     /translation="MALRDIKPTRSELINIKRRIKLSERGYNILKMKRDGLILEFFKV
                     LQQAKDSRGALTERYTHAMEMIALAETVEGAIGVKAAAFSASDIPAITLASKNIMGVV
                     VPEIEASSVRKGILDRGYGMLGTSAVIDETAEAFEDLLEAVIEAAEIETTMKRLLDEI
                     ESTKRRVNALEFKVIPELTEARDFIKMRLDEMEREELFRLKKIKARSA"
     gene            complement(3028..4413)
                     /locus_tag="PLV90_05370"
     CDS             complement(3028..4413)
                     /locus_tag="PLV90_05370"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016469 - proton-transporting two-sector
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit B"
                     /protein_id="HOF96579.1"
                     /translation="MKEYRTVAQIAGPLVFVEKTEPVGYSELVNIVTADGTVKRGQVL
                     DTSDEVVVVQVFETTAGIGRDSGIRFTGETIKMPVGKDMLGRILSGGGKPIDGGPEIV
                     PEKRLEITGAAINPYARASPADFIQTGISTIDGTNTLVRGQKLPIFSGSGLPHNDVAL
                     QIARQAKVPGSKEEFAVVFAAMGITREEANYFMADFEKTGALERAVVFLNLADDPAVE
                     RIITPRLALTTAEYLAFDLGYHVLVILTDMTNYCEALRQIGAAREEVPGRRGYPGYMY
                     TDLASIYERAGIIKGVKGSVTQIPILTMPGDDITHPIPDLTGYITEGQIVVNRDLHRK
                     GIYPPINVLPSLSRLMNLGIGKGHTREDHKKVSDQLYAAYAEGNDLRGLVAIVGKDAL
                     SERDRMFLEFADLFEDRFVRQGLYEDRSIVETLDLGWDLLATLPEEQLVRIDRELIQK
                     YHPKYRKKAQG"
     gene            complement(4416..6170)
                     /locus_tag="PLV90_05375"
     CDS             complement(4416..6170)
                     /locus_tag="PLV90_05375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016469 - proton-transporting two-sector
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit A"
                     /protein_id="HOF96580.1"
                     /translation="MKKSKENGAQGALKRISGPVVTAVGIDAHMYDVVKVGNEELMGE
                     VIKIQGENVIIQVYEDTAGIRPGEPVTNTGLSLAVELGPGLLTSIYDGIQRPLEVLVD
                     KMGNFIERGVSAPGLSHEKKWEFVPTVKAGDVVKAGDILGTVQETNIVHKVMVPPNAK
                     GGKVKKIAAGSFTVDETVCTLEDGTAITMLQRWPVRVPRPVKQKLNPDIPLITGQRIL
                     DGLFPIAKGGTAAIPGPFGSGKTVTQQQLAKWSDAEIVVYIGCGERGNEMTEVLTEFP
                     ELEDPKTGKPLMERTVLIANTSNMPVAAREASVYTGITIAEYFRDMGYDVSLMADSTS
                     RWAEAMREISSRLEEMPGEEGYPAYLAARLSEFYERAGRAINLNGAGGSVSVIGAVSP
                     PGGDFSEPVTQNTLRIVKVFWALDAKLSQRRHFPAINWLNSYSLYLDSLTGWYDREVS
                     PEWNPLRAWAMGVLQKEAELQEIVQLVGSDALPDEEQVTIEVARMIREIFLQQNAYDA
                     VDTFCPMSKQYDMMKAIKFYADLARTAQAGGATPQQVIGVKSKNELVQIKFIKDYEPV
                     LAQIRKDMEAEFDAVRAA"
     gene            complement(6170..6472)
                     /locus_tag="PLV90_05380"
     CDS             complement(6170..6472)
                     /locus_tag="PLV90_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOF96581.1"
                     /translation="MEIAVVGSGEFILGFRLAGVRRTYAAETDERLVEHINRVLEDKD
                     VGILVLKGSDMERVPLRLRTVLENSVKPTVIAIGGEEGGLSMRERIKRSVGVDLWK"
     gene            complement(6475..7542)
                     /locus_tag="PLV90_05385"
     CDS             complement(6475..7542)
                     /locus_tag="PLV90_05385"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HOF96582.1"
                     /translation="MAGISSSSGFATNYIYACTRMRVRRSRLIPREDYIRMLNMSLPE
                     ITRFIGAEGGYGSEIDEHGTSFSGINLVEVALGWNLAKEYQKILELVPGELKYFTASY
                     LRRWDIQNVVTVLRGKMQEMSPGRIKEVLVPAGRLDRVALDRLIAEESLERVVEALRG
                     EQFYPVLERELPRAIETGSFAHLENELYKGYYARLIADAKAGVKGGGVFLKYIELEID
                     TRNIQNLFRLRAGNVHEDVRELMIPGGSFSVEELQRLSGLEDKDEFIDALKKQVRSVP
                     LLNTLETIRGKTALHAIEVALTRVQLDQMVRISKRYPFSVLPVIVYLEEKKYEVANLR
                     ALARGKEAGLPGERLQGYLVM"
     gene            complement(7555..8133)
                     /locus_tag="PLV90_05390"
     CDS             complement(7555..8133)
                     /locus_tag="PLV90_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HOF96583.1"
                     /translation="MGLEAVVNEIREKGRREVEAVRAETRADVEEILKDAQARAAAIK
                     VSAEEEANRAAAHITNQETSAANLVVKRQILNAQKTLLDQVYSASLAAVGDLPAEFHE
                     KALTDLLKRAAKEIEKGTVHANRRDTPVVKKIFGATKSLSGYTVGAPVEISGGIIVES
                     TDGDLQIDYSYRMFLDEIWESALKDASDILFT"
     gene            complement(8145..8393)
                     /locus_tag="PLV90_05395"
     CDS             complement(8145..8393)
                     /locus_tag="PLV90_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOF96584.1"
                     /translation="MADVVMTLEMVQASQMGMKALGAALAVGLTGIASGVAEMTIGSA
                     AVGATAENRDVFGLVLLLTVIPETIVIFGLVVALLLLF"
     gene            complement(8398..>9841)
                     /locus_tag="PLV90_05400"
     CDS             complement(8398..>9841)
                     /locus_tag="PLV90_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HOF96585.1"
                     /translation="VDGNMIAVVVPNEHREEVERTLLDSGFQAIPVPEEAGAPADRLK
                     NHIDEAREATTGIAGVNQRLAEIRDRHADLLVACDELLTADVERAEAPLRFATTEETF
                     ITEGWVPEDRADGIRAALEKATNGRIYIGELAIDDDAKVPVEYENPSFASPTQVLMDI
                     YARPRYSELDPTLMVAIVFPIFFGLILGDVGYGAILLALSLGLRKFLKGDEGRMLLSV
                     LMYASVSSIIFGILYSEFFGAALPWPALIFNRHLNIGTVGGHPPQITELMILAIWIGI
                     LHITLGRILGMVNARKFHHGRHAAKAAIANAGWIGTMWGILLMIWAFYAIPLMPDLTG
                     LPVVAMGLNVAAVAGAALLVAGILGIFQENALELVEIPTIISHVLSYARLVAVGLSSV
                     AIAMVVNYIAIGMMIQPQLEAITPLGVILIIFGVLVLFMGHALNTALGLLGGGLHSLR
                     LHYVEFFTKFYKGGGKKYNPFGMKSKFTEE"
     assembly_gap    9842..9941
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9942..10514)
                     /locus_tag="PLV90_05405"
     CDS             complement(<9942..10514)
                     /locus_tag="PLV90_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HOF96586.1"
                     /translation="MLRPEGMRRLLIAVPKGEIDTVIRELYRHNIYHIEDFVPESESP
                     DELSIGHPLPGASDAASALIKVRAIENACGIDPDSVEIKEKIPASKVRERIRTDLPAI
                     QKEVEGLVATRSKLEAQKKEHEQRAKELEPFAALPLDLELYRGYIRFTVFTGHIRHDV
                     AIDVPHEKYFSDRVDGNMIAVVVPNEHREEV"
     gene            complement(10501..10830)
                     /locus_tag="PLV90_05410"
     CDS             complement(10501..10830)
                     /locus_tag="PLV90_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit subunit G family protein"
                     /protein_id="HOF96587.1"
                     /translation="MKTDVLKSIRETEEEYQAMIRDAQAGRKKSLSDAELEADNLVQK
                     AQSDAGDYRNRRLAEARAQAQNRYAEIVREGEARAETLKAQGGKNLTRAVDFVVSRFK
                     EHLHVKA"
     gene            complement(10882..11418)
                     /locus_tag="PLV90_05415"
     CDS             complement(10882..11418)
                     /locus_tag="PLV90_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOF96588.1"
                     /translation="MSKRPEGGTSGSPPAAPGEAAVPLAGEILIIRRGGSFLLVGKAG
                     SRFTLCIETPDDEYCLAVDPDDLVVVSMPEGGPIEQARMMLELVRQYHIPLVVLPKDH
                     PGSKRLSMVVSVAPEILLACDVRRGTHPEQHLLCSSAELSGLSLAGVPGGITLKQLPP
                     GATVERLNPEKSSTDKQQ"
     gene            complement(11402..11554)
                     /locus_tag="PLV90_05420"
     CDS             complement(11402..11554)
                     /locus_tag="PLV90_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96589.1"
                     /translation="MCTVGGPVDIEFNERVKGRNYRCKECGERFKGIGKRPMCPSCQS
                     EDVEEA"
     gene            complement(11695..>12668)
                     /locus_tag="PLV90_05425"
     CDS             complement(11695..>12668)
                     /locus_tag="PLV90_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOF96590.1"
                     /translation="DEFDDVRVEERTGDEVQVRQLQRYDRIRNIRAFLRHPLKVFLVD
                     PNRTFYVTVPVALVYVLLAFFMTPAYTDVEVLIDVLDDHIIIALLIILVPFGAFHWFW
                     QKTVMGLEAAIPEFLNRLSGINQVGLTLVQAITIVVKADLGVLTYEIKKIKRDIDWGA
                     SIQDALVRFEERIRTPAIARAVTLITTASRMTGDIGEVLNIAARDAAMSETLKRERRG
                     EMFIYVAIVYLVFIVFLFVVIVIDTQFLSALAETEALSPGGYSAGISFGKTPVITFER
                     LLFHACLIQALFSGLIAGEMGEGSLRAGVKHAAVMVFIAFVVFTIAL"
BASE COUNT         2345 a         3959 c         3710 g         2554 t
ORIGIN      
        1 caggcgaaca acggggatgc actggcagtc tgccggaggg tgcgcggcag ccttgcgacg
       61 tttcacgatc tcgtgctcgc gtcggtcatc ggcaacacgc tggactacgg ctcaaaggcc
      121 cataccgtca ccgacgactt cgtcgggttc ttccaccggg agttcgcctc agggctgacc
      181 gtcgacgata ccgaccggat ggagcccctc accgcccggg tggtctacct cgccgacaac
      241 tgcggggaga tcgtctttga cgccctgctt gccgaacacc tcaggaagag cggatcacac
      301 gtcacgtttg ccgtcagagg cgcgccgatc ttaaacgacg cgacgatcga ggatgcgctg
      361 gcgctcgggc tcgaccgccg ggtggatatc ctcaccacca ccacggatgg gatagccgag
      421 ctcggtctca accgggatct tcttccaccc atcctcgccg acgcgctcga ccgcgcgacg
      481 cttgtcatcg cgaagggcat ggcaaactac gagtcgctct ccgacgaggg cgacctcccg
      541 ccggtggctt accttatgtc ggtgaagtgc ggcccgatcg gcgcggatat cggtatccct
      601 gtcggttccc gggtcgccct cctccgcgag tgatccggcg gcagcgcggg ccgggggata
      661 ccccgggcag cgttgcacca atcccaaaga taacatataa agaatatggt tacagaaaaa
      721 gattatttaa acaattttta tgattaaaag aacggtttaa gacatcattt ttgcaatttg
      781 tcgtcatata tccacaatat aattatagga aaatattata tagccaataa tagagacatt
      841 ggatcggggt acgaccacac ccagcgaccg tcagtcattt caatttccat cagcaacgac
      901 cacaaacacc acacacctat cagcaataca gggataacaa cgagggacgc tccgggcaca
      961 acacgacgcg cccctgcacc agtattcatc ggctacaagc gagatctcac cgggctcacg
     1021 aagtgttcgg gtgtgggctc cccacctcat tttttcggga accagaaccg gggagccacg
     1081 ggtgacacat aacggggtgt gacggcgggg gtcacctgcc gcccgttcca cggtgccggg
     1141 cacggaccgc gccctccttc acccgaagat cttcttcgac atctccagcg cctcaagggc
     1201 gggcatcttc ttcccggtca ggaactcgat cgccgccccg ccgccggtgg atatgtgagt
     1261 gaaccggtct tcgatgccga gccgctcgat ggccgcgccg gaatgcccgc cgcccaccac
     1321 cgagaacttg acggtcgagg ccgccctcag gagatcgaac gtgccgacgg cgaactgctc
     1381 ttcctcaaag agccctgcag ggccgttcac cacgaccgtc cccgacgccg ataccgcctc
     1441 tgtgaggcgg tcgatcgact cgctcccgat gtcgagcacc tgggcatcct ccgggacggc
     1501 atcgaccggg tactccgccc gcccgccgtc ctcgcggacc gcaaccgaat gaggcatcag
     1561 gatccggtcc cggtaggtct taaggagatc tcgggcgcgc tcgatctcgc cgcggtagcc
     1621 gaggtcgtcg atcaggcggg ccgaaggccg gccgatatcg tagccggccg cgagcaaaaa
     1681 gacgtttccg accacgccga ccacgatgac gcggtcggcc gtcccgcgca gaaggacatt
     1741 ctctgcgacc gcgatcgagt cgtccacctt cgtcccccca aggacgaagg tgacggggcg
     1801 cggggcgccg gtaaagaccc gggagaggtt tgcaacctct ttctccatca ggaggccggc
     1861 caccgacgga agcagaagcg gcagccccac gatcgagggc tgcgagcggt gcgccgtgcc
     1921 gaacgcatcg ttgacgtaga agtcggccat cgcggcgagc ctccgtacga ggatggtctt
     1981 tttggcctct tccggtttca gggtcaggtt ctcctcggcg gcaaaccgga ggttctcgag
     2041 catcaggata tctccgcgct tcgcgccccg gatagcatcg cgggcgcacc gcccgaatac
     2101 atcgtccacg taggtcaccg ggcggccgag cagccgctcg aacttcgccg cgtgcgcttc
     2161 aagcgtcgtg taatccttct tgccgggcct gctctggtgc gtgaggatga caagccgcgc
     2221 atcctcgagt gcctgcaccg tcggcaggtg ctcccgaaac ctcttgtcgt ctaatatctg
     2281 gttagacgag gggtcgatgg gggagttgag atccacccgc agcaacaccg tccccttcag
     2341 tttccctgca tcagcaagcg taccgatatc caccgggtat cacctgtacc ctactcaggc
     2401 gctccgtgcc ttaatctttt tcaggcggaa gagctcctcg cgctccatct catcgagccg
     2461 catcttgatg aagtcgcgcg cctcggtgag ctccgggatc accttgaact cgagcgcgtt
     2521 cacgcgccgc ttggtgctct cgatctcatc gagcagccgc ttcatggtcg tctcgatctc
     2581 ggccgcctcg atgaccgcct cgagcagatc ctcaaacgcc tccgcggtct cgtcgatgac
     2641 ggcggatgtg ccgagcatgc cgtagccgcg gtcaaggatg cccttcctga cggaagacgc
     2701 ctcgatctcc gggacgacga cgcccatgat gttcttgctt gcgagggtta tggcggggat
     2761 gtcggatgcc gagaatgcgg cggccttcac cccgatcgcc ccttccacag tctctgcaag
     2821 ggcgatcatc tccatagcgt gcgtataccg ctccgtcagt gcgccgcggc tgtctttggc
     2881 ctgttgcagc accttgaaga actccaggat cagcccatcg cgcttcatct ttaagatgtt
     2941 gtagccgcgc tccgagagct tgatccgtcg cttgatgttg atcagttcag agcgggtcgg
     3001 cttgatgtct cgcagcgcca tggatgctta cccctgggcc ttcttgcggt acttcgggtg
     3061 atacttctgg atcagttcgc ggtcgatacg caccagctgc tcctcgggca gcgtcgcgag
     3121 gagatcccag ccgagatcga gcgtctcgac gatcgaccgg tcctcgtaga ggccctgcct
     3181 gacgaaccgg tcctcgaaga ggtcggcgaa ctcaaggaac atccggtcgc gctccgagag
     3241 cgcatccttg ccgacgatgg cgacaagccc ccggaggtcg ttgccctcgg catacgccgc
     3301 atagagctgg tcggagacct tcttgtgatc ctcgcgggtg tgccctttcc cgataccgag
     3361 gttcatcagg cgggagagcg acggcagcac gttgatcggc gggtagatgc ccttccggtg
     3421 cagatcacgg ttgaccacga tctggccttc ggtgatgtaa ccggtgaggt ccgggatcgg
     3481 gtgggtgata tcgtcgcccg gcatcgtcag gatcgggatc tgggtcaccg agcccttgac
     3541 gcctttgatg atgccggcac gctcgtagat gctcgcaagg tccgtgtaca tgtaacccgg
     3601 gtagccgcgt cgtccgggca cttcctcacg tgccgcgccg atctggcgga gcgcctcgca
     3661 gtagttggtc atatccgtca ggatgacgag cacgtggtag ccgaggtcga acgccaggta
     3721 ctctgccgtg gtgagggcga gacgcggcgt gatgatccgc tcgactgccg ggtcatccgc
     3781 gaggttcaag aagacgaccg cccgttcgag ggcgccggtc ttctcgaagt cggccataaa
     3841 gtagttggcc tcttcccggg tgatacccat cgccgcaaag actacggcga actcttcctt
     3901 cgacccgggc accttggcct gacgggcgat ctggagagcc acgtcgttgt gggggagacc
     3961 cgaccccgag aagatcggga gtttctgacc ccggacaagg gtgttcgtcc cgtcgatcgt
     4021 cgatataccc gtctggatga agtccgcggg agacgcacgg gcgtaggggt tgatggccgc
     4081 gccggtgatc tcaagcctct tttcgggcac gatctccggg ccgccgtcga tcggcttgcc
     4141 gccgccggag aggatacgcc cgagcatatc cttcccgacc ggcatcttga tcgtctcgcc
     4201 ggtgaagcgg atgcccgaat ccctgccgat acctgcggtg gtctcgaaga cctggacgac
     4261 aacgacttcg tcgctcgtgt ccagcacctg gccgcgcttg accgtgccgt cggcggtcac
     4321 gatgttgacg agctcgctgt atcccaccgg ctccgtcttc tcgacgaaga ccagggggcc
     4381 tgcaatctgt gcaaccgttc tatactcctt catgatcacg ccgcccttac tgcatcgaat
     4441 tcagcttcca tatccttccg gatctgtgca agcaccggct cgtagtcctt gatgaacttg
     4501 atctgaacaa gctcgttctt gctcttcacg ccgatgacct gctgcggggt tgctccgcct
     4561 gcctgggcgg tgcgcgcgag atcggcgtag aacttgatcg ccttcatcat gtcgtactgc
     4621 ttggacatcg ggcagaacgt atccaccgca tcgtaggcgt tctgctgcag gaagatctcg
     4681 cggatcatcc gcgccacctc gatggtgacc tgctcctcat cgggcagggc atccgaaccg
     4741 accagctgga cgatctcctg gagttcggcc tctttctgga ggacgcccat cgcccacgcc
     4801 cggagcgggt tccactcggg cgagacctcg cggtcgtacc acccggtgag cgagtcaagg
     4861 tagagcgagt aggagttcag ccagttgatg gccgggaagt gccggcgctg ggagagcttc
     4921 gcatcgagcg cccagaagac cttgacgata cggagagtgt tctgggtgac cggctcggag
     4981 aagtcgccgc cgggcggaga gaccgcgccg atgaccgaga ccgagccgcc cgcaccgttt
     5041 aagttgatgg cgcggccggc acgctcgtag aactccgaga ggcgcgccgc aaggtatgcg
     5101 gggtacccct cttcgccggg catctcctca agacggctcg agatctcgcg catggcttca
     5161 gcccaccgcg acgtggagtc ggccataagg gagacgtcgt agcccatgtc gcggaagtac
     5221 tcggcgatcg tgatccccgt gtagacggat gcttcacggg ccgcgaccgg catgttggat
     5281 gtgtttgcga tcaggaccgt ccgctccatc agcggtttgc ctgtctttgg gtcctccagt
     5341 tccggaaact cggtcagaac ctcggtcatc tcgttgccgc gctcaccgca gccgatgtag
     5401 acgacgatct cggcgtcgga ccactttgca agctgctgct gggtgaccgt cttgccgctc
     5461 ccgaacggtc cggggatggc cgccgttccg ccctttgcaa tgggaaagag accgtccagg
     5521 atccgctgac cggtgatcag cgggatgtcc gggttcagct tctgcttcac gggccggggc
     5581 acccggaccg gccagcgctg gagcatcgtg atagcggtgc cgtcctcaag ggtgcagacc
     5641 gtttcgtcca ccgtgaaact gccggccgcg atcttcttga ccttgccgcc ttttgcgttc
     5701 ggcgggacca tgaccttgtg gacgatgttc gtctcctgga ccgtaccgag tatgtcgccg
     5761 gccttgacga catcacccgc cttcaccgtg ggcacgaact cccacttctt ctcgtgcgag
     5821 aggccggggg ccgagacgcc gcgctcgatg aagttgccca tcttgtccac gagcacctcg
     5881 agcggccgct ggatcccgtc gtagatactg gtcagcaggc cgggcccgag ctccacggcg
     5941 agcgagaggc cggtgttcgt caccggttcg ccgggcctga tgccggcggt gtcctcgtag
     6001 acctggatga tgacgttctc accctggatc ttgatgacct cccccatcag ttcctcgttg
     6061 ccgaccttca ccacgtcgta catgtgtgcg tcgataccaa cggcagtgac gaccggccct
     6121 gagattcgtt tcagggctcc ctgagccccg ttctccttgc ttttcttcat tacttccaca
     6181 gatcaacacc caccgatctc ttgattctct ctctcatcga caggccgccc tcctctccgc
     6241 cgatggcgat caccgtcggc ttaacggagt tctcgaggac ggtgcggagc cgcaggggga
     6301 cccgctccat gtcactgcct ttcagcacaa ggatgccgac gtctttatcc tccagtaccc
     6361 ggttgatatg ctcgaccagc cgctcgtcgg tctcggccgc gtaggtcctc ctgacgcccg
     6421 cgagccgaaa accgagtatg aattcaccgc tcccgacaac tgcgatctcc atgcttacat
     6481 caccaggtag ccctgtaatc gctcgccggg gaggccggcc tccttgcccc gggcgagggc
     6541 gcgaaggttc gcgacctcat acttcttctc ctccaggtag acgatgacgg gcaggaccga
     6601 gaacggatac cgcttcgata tccgcaccat ctgatcgagc tggacccggg tcagcgcaac
     6661 ctcgatcgca tgcagggcgg tcttgccccg gatggtctcg agcgtgttca ggagcgggac
     6721 cgatctcacc tgctttttta aagcgtcgat gaactcgtct ttatcctcaa gccccgagag
     6781 ccgctgcagt tcttccaccg agaacgagcc gccgggaatc atcagttccc gcacgtcctc
     6841 atggacgttc ccggcccgga ggcggaagag gttctggatg ttccgggtat cgatctcaag
     6901 ctcgatgtac ttcaggaata cgcccccgcc cttgacgccc gctttcgcat cggcgatgag
     6961 ccgcgcgtag tagcccttgt agagctcgtt ctccaggtgg gcaaacgacc cggtctcgat
     7021 cgcgcggggg agttcccgtt cgaggaccgg gtagaactgc tcgcccctga gggcctcgac
     7081 gacccgttcc agtgactctt cggcgatcag gcggtcgaga gcgacccggt caagcctgcc
     7141 ggccgggacc aggacctcct tgatcctgcc cggcgacatc tcctgcatct tgccgcgcag
     7201 gacggtgacg acgttctgga tatcccagcg gcgcaggtaa ctcgcggtga agtacttcag
     7261 ttccccgggt acaagctcca ggatcttctg gtactccttt gcgaggttcc agcccaatgc
     7321 cacctcgacg aggttgatac cggagaagga ggtgccgtgt tcatcgatct cggacccgta
     7381 accgccctcg gcgccgatga accgggtgat ctcaggcagg ctcatattca gcatccggat
     7441 ataatcttcg cgcggtatca gccgtgacct ccttacgcgc atgcgggtgc aggcgtagat
     7501 gtagttggtt gcaaatccgc tgctactact tatccctgcc atgtatgaac cccctcatgt
     7561 gaacaggata tccgacgcat ccttcagggc ggattcccag atctcatcca ggaacatgcg
     7621 gtagctgtag tcgatctgca ggtcgccgtc ggtgctctcg acgatgatgc cgccggagat
     7681 ctctacgggc gcgcccaccg tgtagcccga gagagacttt gtcgcgccga agatcttctt
     7741 gacgaccggg gtatcccgcc ggtttgcatg cacggtgccc ttctctatct cctttgccgc
     7801 tctcttcaac aggtctgtga gcgccttctc gtggaactca gcaggcagat caccgacagc
     7861 agcgagcgag gccgaataga cctgatcgag gagcgtcttc tgggcgttca agatctgccg
     7921 cttgacgacg aggtttgctg ccgaggtctc ctggttggtg atgtgggcgg cggcccggtt
     7981 cgcctcctct tcggccgaaa ccttgattgc ggcagcccgg gcctgtgcgt ccttcaggat
     8041 ctcctcgacg tcggcccggg tctctgcccg gaccgcctcg acctctcttc gccctttctc
     8101 ccggatctcg ttgacgactg cttcgagtcc catggtccac tccatcagaa cagaagcagc
     8161 agtgcaacca caagaccgaa gatgacgata gtttcgggaa taacagtaag cagcagtacc
     8221 agaccgaata cgtcgcggtt ctccgccgtc gccccgaccg cagcggaacc gatcgtcatc
     8281 tctgcaacgc ccgatgcaat gccggtaagg ccgacagcaa gagccgcacc gagtgccttc
     8341 attcccatct gcgatgcctg taccatttca agagtcatta caacatctgc catttcttta
     8401 ttcctccgta aactttgatt tcatgccaaa cgggttgtac ttcttgcctc cacctttgta
     8461 gaacttggtg aagaactcaa catagtgaag acgaagtgag tgcagaccgc ctccaaggag
     8521 gccgagtgct gtgttcagcg cgtgccccat aaagaggacg aggacgccga agatgatgag
     8581 gatcacgccg agcggtgtga tcgcttcaag ctggggttgt atcatcatgc cgatcgcaat
     8641 gtagttgacc accatcgcga tagcgaccga ggataagccc accgccacga gacgggcgta
     8701 cgacaataca tgactgatga tcgtcgggat ttcgaccagt tcgagcgcat tctcctgaaa
     8761 gatcccgagg atgcctgcca ccagaagggc ggctcccgcc acggcggcaa cattgagccc
     8821 catggcgaca acgggaagac ccgtcagatc gggcatcaac gggatggcgt agaacgccca
     8881 gatcatgagg aggattcccc acatcgttcc gatccacccg gcattggcga ttgccgcttt
     8941 tgccgcgtgt ctcccgtggt ggaacttccg ggcgttgacc ataccgagga tgcgtccgag
     9001 cgtgatgtgc aggatgccga tccagatcgc aaggatcatg agttcggtga tctgaggggg
     9061 gtgaccgcct acggtcccta tgttgaggtg ccgattgaag atcagcgccg gccacgggag
     9121 tgcagcccca aagaactcgc tgtagagaat cccgaatatg atgctcgata cgctcgcata
     9181 catgagcacc gaaagaagca tccttccttc gtcacccttc aggaacttcc ggagcccgag
     9241 gctcaaggca agcaggattg cgccgtaccc cacgtcgccg aggatcagcc cgaagaagat
     9301 ggggaacacg atggctacca tcagtgtcgg gtcgagctcg gagtaccggg gtcgcgcgta
     9361 gatatccatg agcacctggg tcggtgaggc aaacgacggg ttttcgtact ccaccgggac
     9421 ttttgcgtcg tcatcgatcg ccagttcccc gatgtagatc cgaccgttgg tggccttctc
     9481 cagtgccgcc cggatcccgt ccgcccgatc ctcaggcacc cagccttcgg tgatgaaggt
     9541 ctcctccgtg gtagcaaacc gcaacggggc ttccgcccgc tctacgtcgg ccgtgagtag
     9601 ttcatcgcat gctaccagga gatcagcgtg cctgtcccgg atctctgcaa gcctctggtt
     9661 gaccccggcg atcccggtag tggcctcccg ggcctcgtcg atatggttct tcagacgatc
     9721 agccggagct cccgcctctt cagggacggg aatcgcctgg aacccggaat caagaagagt
     9781 ccgctcgacc tcttcgcggt gctcgtttgg caccacgacg gcgatcatgt tgccgtccac
     9841 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngacctcttc gcggtgctcg
     9961 tttggcacca cgacggcgat catgttgccg tccaccctgt cggaaaaata tttctcgtgg
    10021 gggacgtcga tggctacatc gtgcctgata tgcccggtaa agacagtaaa ccggatatat
    10081 ccccgataaa gttccaggtc aagagggagc gcggcaaacg gttcgagttc ttttgcgcgc
    10141 tgctcgtgct ctttcttctg cgcctcaagc ttcgagcgtg ttgcgacgag gccttccacc
    10201 tccttctgga tggcgggaag gtccgtcctt atccgctccc tgaccttcga tgcgggaatc
    10261 ttctctttga tctcaacgct atcgggatct atcccgcacg cattctcgat ggctctgacc
    10321 ttgatgagcg ccgaggctgc atcgcttgct cccgggagcg ggtgaccgat cgataactca
    10381 tcgggagact cgctctccgg cacaaaatct tcgatgtggt agatgttgtg ccggtagagt
    10441 tccctgataa cggtatcgat ctcacccttc gggactgcga tgagcagtcg gcgcatccct
    10501 tcaggcctta acatgcaggt gctccttgaa ccgcgagacg acaaagtcta cagctcgtgt
    10561 gaggtttttg cctccctgcg ctttgagcgt ctctgcgcgc gcttcaccct ccctgactat
    10621 ctctgcgtac ctgttctgcg cctgggcccg tgcctctgca agacgccgat tcctgtagtc
    10681 cccggcatcg ctctgtgcct tctggaccag gttatcggcc tccaactcgg catccgagag
    10741 gctctttttc ctcccggcct gcgcatcacg gatcattgcc tgatactctt cttcagtttc
    10801 cctgatgctt ttcaggacat cggtcttcat caaaccctcc ttctcacggc ttataagtat
    10861 ttgatgaaaa tgagcatata tttattgttg tttgtccgta gaggatttct ccggattcag
    10921 acgttctact gtcgccccgg gggggagttg tttcagtgta atacccccgg gaaccccggc
    10981 aagcgagagc ccggagagtt ctgccgacga gcagaggagg tgctgctcgg ggtgggttcc
    11041 tcgccggacg tcgcaggcaa gcaggatctc cggagcgacc gagacgacca tcgaaagccg
    11101 ttttgacccc ggatgatctt ttggaagaac gacgagcggg atatggtact gccggaccag
    11161 ctcgagcatc atgcgtgcct gctcgatagg cccgccctcc ggcatcgaga cgacgacgag
    11221 atcgtcgggg tcgaccgcaa ggcagtactc gtcgtcaggg gtctcgatac agagggtaaa
    11281 ccgggaaccg gccttcccca cgagcagaaa agagccacct ctcctgatga tcaggatctc
    11341 tcctgcgagg gggacggcag cctcgcccgg cgcggcggga ggactcccgg atgtgccgcc
    11401 ttcaggcctc ttcgacatct tccgactggc agctcgggca catgggccgc tttccgatgc
    11461 ccttaaaccg ctcgccgcac tccttgcagc gataattcct gcctttcacc cgttcgttga
    11521 actcgatatc gacaggtccg cctacagtac acatcttgtt atcaccagga gagtgatctc
    11581 tagcggagaa tataaaattc tatcgcgaaa cccacggcac ggtgaggcgg aaggctgatg
    11641 cccgatagag aggagttccc gcacgggccg gccggaggtg gagatcgggg actattatag
    11701 ggcaatagta aacacaacga acgcgatgaa caccatcacg gcggcgtgtt tgacccccgc
    11761 ccggagcgat ccctctccca tctccccggc gatcaggccc gagaagagcg cctggatgag
    11821 acacgcatgg aagaggaggc gctcgaacgt gatgacgggg gtctttccga acgatatccc
    11881 ggcggaatat ccgccgggag agagagcctc agtctctgcg agcgcggaga ggaactgggt
    11941 atcgatgacg atcacgacga agaggaagac gatgaagacc aggtagacga tggcgacgta
    12001 gatgaacatc tccccccgcc tctcgcgctt gagagtctcc gacatcgcgg cgtcgcgggc
    12061 ggcgatgttc agcacctccc cgatgtcgcc ggtcatccgg cttgccgtcg tgatcagggt
    12121 gaccgcccgg gcgatggcgg gagtgcgtat ccgctcctcg aaccggacaa gcgcatcctg
    12181 gatgctcgca ccccagtcga tatcgcgctt gatcttcttg atctcgtagg tcagcacccc
    12241 gagatccgcc ttcaccacga tcgtgatggc ctgtacaagg gtcaggccca cctggtttat
    12301 gcccgagagg cggttgagga actcggggat ggctgcctca agccccatca ccgtcttctg
    12361 ccagaaccag tggaaggcgc cgaacgggac aaggatgata agcagcgcaa tgattatgtg
    12421 gtcgtcgagg acgtcgatca gcacctcgac gtcggtgtag gccggggtca tgaagaaggc
    12481 aaggagaacg tagacgagcg cgaccggcac cgtcacgtag aacgtccggt tcggatcgac
    12541 gaggaatacc ttgagcggat ggcgcaggaa ggcgcggatg ttcctgatgc ggtcgtagcg
    12601 ctgcagttgc cgaacctgta cttcgtcgcc ggtccgctcc tcgacccgga cgtcgtcgaa
    12661 ctcgtcga
//
LOCUS       DAOSII010000053        12606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_5036_length_12606_cov_47.726237, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000053 DAOSII010000000
VERSION     DAOSII010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 12606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12606
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5036_length_12606_cov_47.726237"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PLV90_05430"
     CDS             <1..286
                     /locus_tag="PLV90_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96591.1"
                     /translation="LVPVLATHGNTSVEEFSRRSGGTYTTVVFLDGFVPQENATPISF
                     DLRYQGGGGIKHLIKENVWTTTMNATVPSTESGFVPVPAGYHVTPGGIYL"
     gene            487..906
                     /locus_tag="PLV90_05435"
     CDS             487..906
                     /locus_tag="PLV90_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOF96592.1"
                     /translation="MAARVEQLIEVFDRLIAIRNECSDEILAECGLSRLTVKQVAYLK
                     AIDEHGDVTFSALAGITQTSKPTVTEMVDRFVRMECAYRQRCPDDGRVVYIRLTEKGR
                     MIANAEQNALYRMIERMVEALDENEVDLLLEILKKVG"
     gene            962..3340
                     /gene="lon"
                     /locus_tag="PLV90_05440"
     CDS             962..3340
                     /gene="lon"
                     /locus_tag="PLV90_05440"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOF96593.1"
                     /translation="MHASQSDTSETIVIPLFETVVYPETRTKLQVEAAVGKVLIAAMK
                     SDGSASAVGLTVKGGAEPPENPADAFYATGTLLAIAHVQPTDDGYLIFAHATRRVKAV
                     TLSEKDGQFYTTFEPLPDLPDLDEDARADMLADIKAAIHTISGNFQGSEQFTRPVERM
                     ESVDQVMAFVMPFLPVDLATKQSLLETVSVRERSTAFLRILVDLNESINLRIEVAKKA
                     SEKVGKANREAMLREQLRVIQEELNGCDGPAGEEGYRERIERSTMPEEVRKKALAELK
                     KLESGGSQHHESQGIRNYLDLLLALPWTSEKKSIDIEEARRILESNHNGLEKVKERIV
                     QHLAVMKLKEEKQGSILLFSGPPGTGKTSLGRSIADALGREYVRISLGGVKDEAEIRG
                     HRRTYVGSLPGRIIQGMKRAGAKNPVFILDEVDKLAVSYSGDPASALLEVLDPEQNST
                     FSDHYLEVPYDLSDVLFIATANSLATIPAPLLDRMELIEISGYTKNEKFAIAKDHLLP
                     ETLTEHGLTTDHLQVEDDAVSAIIDRYTREAGVRTLKKQLARIARYVSAKVVSGPIDL
                     PVVVTADMLPEILGRETVRPDVARKENPPGVVTGLAWTPVGGDILFIEGTFMPGKGKL
                     TLTGQLGDVMKESAQISLSLIRSRLAATPTRFDFFASDIHIHVPAGATPKDGPSAGVT
                     LFTALASLVTGKAVDPTVAMTGEVTLSGAVLPVGGIKEKVLAAHRAGIKTVILPRENE
                     RDLIDVPEDARDELTFVPVDTIEDLLREALGIELHGPVLPYVGNRCVPAHNL"
     gene            3545..4303
                     /locus_tag="PLV90_05445"
     CDS             3545..4303
                     /locus_tag="PLV90_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96594.1"
                     /translation="MKQSHIASIALTFCIAFFAGALFTNVLAYNPEPPAPSLRQNVYG
                     LPLSEMWAIVRDQTGIENSTAVLGDFWLVIDGDGAVEQLNFEFYGDDAGLLRWYRVAA
                     SSTGKVTWDSRKVEKAPPGEHPLILFTEIERIPYRELADENGILIVSVGSQSGGLGYD
                     AGYRPLFALRQGEIIPLKSVAFNTAEPWYSIDVSHGTKDGSGLVGTHYCTIFTLRDLT
                     RAERVYCPDGELFWPPPGSSPAGVDGLSELGVVS"
     gene            4343..4738
                     /locus_tag="PLV90_05450"
     CDS             4343..4738
                     /locus_tag="PLV90_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96595.1"
                     /translation="MESDNKRLIAVGLLAFVGVAVLVAAVVFGFTTLVALVTGIDGLP
                     QELTVEVVGKEALQNASVVHLTDRDLQQHPVLATAIREAGSDSGVSASAPMTGVECLA
                     LTESFGVYTRDAPILEYDGVYYSTRVLLH"
     gene            4759..5199
                     /locus_tag="PLV90_05455"
     CDS             4759..5199
                     /locus_tag="PLV90_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96596.1"
                     /translation="MEEGKKSGNRLRTAGFVIACALAGIVAVALGLVLADALLIYAME
                     GSGGQPMLYVMESAEPGENVVIPLTEKDFADHPALDAVIRGDERNPSAWEWGRSDRRF
                     IGGANVSYVESRVLQDTYGPDRETWVYPHLEYEGAYYLVVTTWP"
     gene            5219..6319
                     /locus_tag="PLV90_05460"
     CDS             5219..6319
                     /locus_tag="PLV90_05460"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOF96597.1"
                     /translation="MNRAIGTWALLLALLLAIAPAAAADLGPNQHLIPELRVNESIEA
                     IVISAELSLQPEWDGSVTTGIPFGAVMVHTANATTLIFDRDGNHLFSIDDELSAKIPT
                     PAGVEKPCTRVHPLPNDSRVYHHGDVTFVFGTAGEPPILTIIDESPKPDEEPITIEIT
                     SPNEGGVAWIDVAPAHVAVAGEVHAPAGVHSVVVRSGSVEVSCGNAAEFACSVPVSFG
                     KNTITVVATDNRRHRAEKTVNLTVRSGIPPPLIAVSGRVTDAGGSPVPGALVRFESVF
                     DLGGEPIAAMAVTGEDGRYLVEDALGYRQTVTVEKEGYSPLREEVVFENLTNTLDLEL
                     EPASRPVPGFGPAVGVLGLLGARWILRNTKRQ"
     gene            6477..7076
                     /locus_tag="PLV90_05465"
     CDS             6477..7076
                     /locus_tag="PLV90_05465"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOF96598.1"
                     /translation="MSYSEPSTDTILGIRMYETERLLLKVATPDDVAAIVSYLIRNRD
                     FLMETEPPRDDRYYSFEYQAGEILHERKMMENGQLLKLWIVKKENPTQMIGTVSFNTI
                     IRGPFQSCFLGYRLDYAEVRRGYMTEALREAIRVLFGECRLHRVEANILPGNGPSLRV
                     VEKLGFHYEGLALRYLLVNGKWEDHIRMVLLNDRWAGDR"
     gene            complement(7123..7608)
                     /locus_tag="PLV90_05470"
     CDS             complement(7123..7608)
                     /locus_tag="PLV90_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOF96599.1"
                     /translation="MLSRRIFETDFARALQDELARQNMSIRELAERAEIPPATLYKLT
                     SGKADPRLSTVRRIVGVLEPREKSFIAVIAARFLLDDLDNRDIEAGDRKYRIRGYSAD
                     SLDECIVAAVRADKEGALGIICAPILAPIVEKIVDCPVAIMKPQQRTIIEAIETIAKR
                     V"
     gene            7712..8491
                     /locus_tag="PLV90_05475"
     CDS             7712..8491
                     /locus_tag="PLV90_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF96600.1"
                     /translation="MDPTPIREITILPGRSRSGEPERFRAITIRPGDTISIVGPTGSG
                     KSAFINDIEVFAQNDTATGRTVLVNGANPPEEFVRDPAHKPVALITQNTRCLADLAVA
                     EFLAMHVRSRKIEDEDIVGKTIDLANEFTGEAIRPDARMTALSGGQTRSLLVADAVMI
                     AAAPILLLDEVENAGIFKERVIDLLRAGGKAVIFVTHDPLVSLLSARRIVMRDGAVER
                     VIEPGDQENAALREAIRLDGILCRMRDEIRAGNLITEPVGA"
     gene            8488..9195
                     /locus_tag="PLV90_05480"
     CDS             8488..9195
                     /locus_tag="PLV90_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOF96601.1"
                     /translation="MKLAVVAGPPSAGKTAVVRQIIRSLSGENRIAYIKIDVVRAFED
                     EELAAEFGIPARKVYSGDLCPDHAGVMVMRDAIAWAGGEGADLLLVESAGLCLRCSPY
                     TTQALGIVVLSAISGTNTPLKMAAMLALADTAVVTRIDLVSQAEKEVFRERIREVNPY
                     LDIVETNALQGTGMRYLLRAIEECPAITDPDTIVLRGAPPLGVCTLCIGKTEIGWQNH
                     FGVIRKLEGADYLYRGD"
     gene            9199..9810
                     /locus_tag="PLV90_05485"
     CDS             9199..9810
                     /locus_tag="PLV90_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOF96602.1"
                     /translation="MAWQPPGKDCGACGAPTCEAFIALVREGEKDLLDCVFYSTDIAP
                     SRLDARHTGRDILGTEYDFILEPLPGEVSARKIILPFRPDLVEKWEIVPGDIVVGRPT
                     GAGCPVQHVLSVIRANPVTGLLTCLVVGPRASRGGGFKEIEAYHIVGFEGISRTVRRE
                     PTFGMRQRFLPGSCMMNLAHTGVVNMVLAQSSGLHVRVEDIRL"
     gene            9807..11048
                     /locus_tag="PLV90_05490"
     CDS             9807..11048
                     /locus_tag="PLV90_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 16 metalloprotein"
                     /protein_id="HOF96603.1"
                     /translation="MKTINEIEEKIRNGSAVVYTAAEFKRLIREGAEVTAADVDVVTT
                     GTCGVMSGTAAVLSVPVAKPGTFERAERIWANGVPCMPGPCPNERLGIVDMIVSGTAH
                     AGAGYGGGHLFRDIVEGRGIEVVVEAADRSVEATVTLDDFAYARLFTTRTAYKNYTAY
                     VNRQPTRIKTIFSIEGLQGPCREASVSGCGEINPLENDPLGLAIGAGTPVLLNGSVGM
                     VTGEGTRSTSERPNLTVIADMTGMQPRYMGGFKTSAGPECITSLGAAIPVLDDRQIAG
                     LLVLDEGIPLPVADITTRTVLGEGTYADVWQQPDREVTYHPGWCEECSTCAAAAVCPT
                     GAFSREAGIDRDRCLACTACLFACPNDAFEAGEGSLRVRGRRIPITLRQSGRTLAEDL
                     CRDLKEMILDSRFTFTGGGIR"
     gene            11045..12334
                     /locus_tag="PLV90_05495"
     CDS             11045..12334
                     /locus_tag="PLV90_05495"
                     /EC_number="2.5.1.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteate synthase"
                     /protein_id="HOF96604.1"
                     /translation="MREKYLLSCPGCGRTFSDRYTLDCPSGCDALLRTVYAERRLIPR
                     DLPGIFRYKDWLPIEGHLGIDAGAVSYESVAFARELGLSHLTVTFSGYWPERGGRMET
                     CSFKELEAQPTVLRLREKGAGVLQVSSAGNTGRAFCQVSGLTGAPVVVVVPAAAAGRL
                     WTTVPAENVCLITVDGDYSDSIAFGREVCTLPGIVPEGGAKNVARRDGMGTVMLDGTL
                     AAGRLPDAYFQAVGSGTGGIAAWEAAERLIADGRFGSRLPALHLSQNLPFVPMVRAWE
                     AGRREIAAEDMPEAGASIARVSADVLTNRHPPWGVRGGVYDALAASGGRMYAVSNDDA
                     RSAGRLFEEAEEIDLDPAAAVAVASLVRAVEEGFVEPDEHILLNVTGGGYQRAAEDLD
                     RYPVEPFVRVAAGEAFEGDIRDAVRAWLAEQEVAIRA"
     gene            12327..>12606
                     /locus_tag="PLV90_05500"
     CDS             12327..>12606
                     /locus_tag="PLV90_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOF96605.1"
                     /translation="MREGCVLDRLAALGIDTVVSLPCDRTRELCALIPERFRAVDLTR
                     EEDGVGISAGLAMAGARSVLHMQSSGLGNSLNAIMSLTVTFGLPLPILA"
BASE COUNT         2286 a         4023 c         4073 g         2224 t
ORIGIN      
        1 gctcgtgccc gtgcttgcga cccacggcaa cacgagcgtc gaagagttca gccggagatc
       61 gggcgggacc tacaccacgg tcgtctttct cgacggcttc gtcccgcagg agaacgccac
      121 accgatctcg ttcgacctca ggtaccaggg cggcgggggc ataaaacacc tgataaaaga
      181 gaatgtctgg accacgacga tgaacgcgac tgttccgagc acggagtcgg gcttcgtccc
      241 ggtgccggcc ggataccatg tcactcccgg ggggatctat ctatgacggg cgagatacgg
      301 aaccgggcaa ttcccttcag catctttctt cctgcgctgt aacacctctc aaacatcgat
      361 gaagaaggcg gcgaagaacc cggatctgcg aaggccgcgg gcatgcctgc tatggatgag
      421 tcccccggca cccgccacca ccacaacctt tatagtttgg tctacccaaa ctaatgggta
      481 tcaaccatgg cggcacgggt cgagcagctg atcgaggtgt ttgatcgcct gatcgccatc
      541 agaaacgagt gttccgacga gatcctcgcc gagtgcgggc tcagccgttt gacggtgaag
      601 caggtcgcct acctgaaagc catcgacgag cacggcgatg tgacgttcag cgctcttgcc
      661 gggatcacgc agacctccaa acccaccgtc acggagatgg tcgataggtt cgtacggatg
      721 gagtgcgcct accggcagag atgccccgac gacggcaggg tcgtctacat ccgcctgacc
      781 gagaaggggc ggatgatagc aaacgccgag cagaacgcgc tttaccggat gatcgagagg
      841 atggtagagg cgctcgatga gaacgaagtg gatctcctcc ttgagatcct gaagaaggtc
      901 gggtaatttt ttttggccgg caaaaaagtt aggtaaattc aaactaatga ttggagtcac
      961 catgcacgca tcacagtcag acaccagcga aaccatcgtc ataccgctct tcgagaccgt
     1021 ggtctacccg gagacccgga cgaagctcca ggtcgaggcg gccgtcggga aggtgctgat
     1081 cgccgccatg aagagcgacg gatcggcatc tgcggtcggg ctgaccgtaa agggcggtgc
     1141 agaacccccg gagaacccgg ccgatgcatt ctacgcgacc gggaccctcc ttgcaattgc
     1201 gcacgtgcag cccacagacg acggctatct catatttgcg catgcaaccc gccgggtgaa
     1261 ggcagtaacc ctctccgaga aagacggaca gttctacacc acctttgaac cgctcccgga
     1321 tctgccggac ctcgacgagg atgcccgggc ggatatgctc gcggacatca aggcggccat
     1381 ccacacgatc agcggcaact tccaggggtc cgaacagttc acccggcccg tcgagaggat
     1441 ggagtccgtc gaccaggtca tggcgttcgt catgccgttc ctgccggtgg atcttgccac
     1501 gaaacagtca ctcctcgaga ccgtctctgt ccgggagcgc tccactgcgt tcctccggat
     1561 cctggtggac ttaaacgaga gcatcaacct ccggatcgag gtggcgaaaa aagcctcgga
     1621 gaaggtcggg aaggcgaacc gcgaggcgat gctccgcgag cagctccggg tgatccagga
     1681 ggagctcaac ggatgcgacg gccccgccgg cgaggaaggc taccgggagc ggatcgagcg
     1741 ctcgacgatg cccgaggagg tccggaagaa agctctcgcc gaactgaaga aactcgagtc
     1801 gggcgggagc cagcaccatg agagtcaggg gatacgaaac tacctcgacc tcctgctcgc
     1861 tctcccctgg acctccgaga agaagagcat cgatatcgag gaggcccgcc gcatactcga
     1921 gagcaaccat aacggccttg agaaggtcaa ggagcggatc gtccagcacc tcgcggtcat
     1981 gaagctcaag gaggagaaac agggctcgat cctcctcttc tcgggcccgc ccggcaccgg
     2041 gaagacgagc ctcgggcgga gcatcgcgga tgcgcttggg agagaatatg tccggatcag
     2101 cctcggcggt gtcaaggacg aggcggagat acgggggcac cggcggactt acgtcgggtc
     2161 gctccccggc cggatcatcc aggggatgaa gcgggccggc gcgaagaacc cggtcttcat
     2221 cctggacgag gtcgacaagc tcgccgtctc ttactccggc gatccggcaa gcgccctcct
     2281 cgaggtcctc gaccccgagc agaacagcac gttctccgac cactacctgg aggtgcccta
     2341 cgacctctcg gacgtgctct tcatagcgac cgcaaactcg cttgcgacga tcccggcgcc
     2401 gctcctcgac cggatggagc tcatcgagat atcgggctac acgaagaacg agaagttcgc
     2461 tattgcaaaa gaccacctcc tgccggagac cctcacggag cacggcctga ccacggatca
     2521 cctccaggtc gaagatgacg ccgtctcggc gatcatcgac cgctacaccc gggaagccgg
     2581 tgtccggacg ctcaagaagc agcttgcccg gatcgcccgg tacgtctccg cgaaggtcgt
     2641 ctcggggcca atcgacctgc cggtcgtcgt gacggcggat atgcttcccg aaatcctcgg
     2701 ccgggagacc gtccggccgg acgtggcgcg gaaggagaac ccgcccggcg tcgtcacggg
     2761 gcttgcctgg acaccggtcg gcggggacat cctcttcatc gaggggacgt tcatgcccgg
     2821 caaggggaaa ctcacgctga ccggccagct cggcgacgtg atgaaggagt cggcccagat
     2881 atccctcagc ctgatccgct cgcggcttgc ggccacgccg acccggttcg acttcttcgc
     2941 aagcgacatc cacatccacg tgccggcagg ggcaactcca aaagacgggc cgtctgcggg
     3001 cgtgaccctc tttacggctc ttgcctccct cgttaccgga aaagcggtcg acccgacggt
     3061 cgcgatgacc ggggaggtga cgctctccgg cgccgtgctc cccgtcggcg ggatcaagga
     3121 gaaggttctt gcagcgcacc gggccggcat aaagacggtc atcctcccgc gagagaacga
     3181 gcgggacctt atcgatgtgc ccgaggatgc ccgggatgaa cttacgttcg tgcccgtgga
     3241 taccatcgag gatctcctcc gggaggcgct cgggatagaa ctccacgggc cggttctccc
     3301 gtatgtgggg aaccggtgcg tgcccgcgca caacctctaa aaaacctctg aatccgggca
     3361 cacccctctt ttgccccgcc cggcgccctt tttctcgcca tgccccgccc cgggaaccga
     3421 ttgtcccaaa gcggcccgac gtgtagacga tcgtgacaga aatcgtttat aggggaacgt
     3481 cgacctctcc cgcatgggcg ccgcgatctg ccccggagat cgcccatcac cctgaggaaa
     3541 caccatgaag caatcccaca tcgcgagtat cgccctcacg ttctgcatcg cgttctttgc
     3601 cggcgctctc ttcacgaatg tgcttgccta taaccccgaa ccccctgctc cctccctccg
     3661 gcagaatgtc tacggcctcc cgctctcgga gatgtgggcg atcgtccggg atcagacggg
     3721 tatcgagaac tcgacggcgg tcctcgggga tttctggctc gtgatcgacg gggacggggc
     3781 ggtcgaacag ttaaatttcg agttctatgg agacgatgca ggtctccttc gctggtatcg
     3841 ggttgcggcg agttcgacgg gaaaagtgac ctgggactcc cggaaggtcg agaaagcacc
     3901 tcccggagag catccgctca tccttttcac ggagattgag cggattccct accgggagct
     3961 ggccgatgag aacgggatcc tgatcgtcag cgtcggttcg cagtcgggag gcctggggta
     4021 tgacgccgga tatcgacccc tctttgccct gcgccagggg gagatcatcc ccttaaagag
     4081 cgtagcgttc aacacggcgg agccctggta cagcatcgac gtctcgcatg gaacaaagga
     4141 cggttcagga ctggtgggta cccactactg cacgatcttt acgctccgcg accttaccag
     4201 ggccgagagg gtgtactgcc cggacgggga acttttctgg ccgccgcccg gttcgtcccc
     4261 ggcaggtgtc gatgggttat ccgaattggg ggtcgtctct tgatgccgga cggccggttc
     4321 actccggaga cagggtggcc gcatggagtc tgacaataag cgcctgatcg ccgtaggact
     4381 tctcgccttc gtcggcgtcg ccgtcctcgt cgcggcggtc gtgttcggat ttacgaccct
     4441 tgttgccctg gtcacgggga tcgacggtct cccgcaggag ttgaccgtgg aggtggtcgg
     4501 gaaagaggct ctgcagaacg catctgttgt ccacctgacc gaccgggacc tgcagcagca
     4561 cccggtgctt gccaccgcga tccgggaggc cggcagcgat tcgggcgtat ccgcgtcggc
     4621 cccgatgacg ggggtcgagt gcctcgccct caccgagtcc ttcggcgtat acacaaggga
     4681 tgcgccgatc ctcgaatacg acggagtata ctactccacc cgcgttctgc tccattagaa
     4741 cgcttgaagg ggtgcagaat ggaagaaggt aagaaatccg gcaaccggct cagaacggcg
     4801 ggattcgtca tcgcctgcgc ccttgccggg atcgtcgcgg tcgcgctcgg cctcgtgctc
     4861 gccgacgctc tcctcatcta tgcgatggag ggctcgggcg gtcagccgat gctctacgtt
     4921 atggagagcg cggagccggg agaaaacgtc gtcatccctc tgaccgagaa ggactttgcc
     4981 gaccacccgg cgctcgatgc ggtcatccgc ggcgatgagc gaaacccgtc ggcgtgggag
     5041 tggggtagga gcgaccggcg cttcatcggt ggagccaacg tctcctacgt ggagagcagg
     5101 gtgctccagg acacctacgg ccccgaccgg gagacgtggg tctacccgca cctggagtac
     5161 gaaggggcgt actacctggt ggtgacgaca tggccgtgag agagccgggg gaggaagaat
     5221 gaacagagcg atcggaacat gggcactcct cctcgcgctg ctgctcgcga tcgccccggc
     5281 ggcggccgcg gatctcggtc ccaaccagca cctgatcccg gaactgaggg tgaacgagtc
     5341 gatcgaggcg atcgtcatct ccgcggagtt gagccttcag ccggaatggg acgggagcgt
     5401 gaccaccggg ataccgttcg gcgcggtcat ggtccatacg gccaatgcga cgacgctgat
     5461 cttcgatcgc gacggaaacc acctcttctc catcgatgac gaactctctg caaaaatccc
     5521 cacccccgca ggcgtcgaaa agccctgcac ccgggtgcac ccgctcccga acgactcccg
     5581 ggtctatcac cacggcgacg tgaccttcgt cttcggcacg gccggggaac cccccatcct
     5641 caccatcatc gacgagagcc cgaagcccga cgaggagccg atcaccatcg agatcacctc
     5701 cccgaatgag ggcggcgtgg cctggatcga cgtcgccccg gcccatgtcg ccgtcgccgg
     5761 ggaggtgcat gcccctgcgg gagtgcacag cgtggttgtc cggagcggtt cggtggaggt
     5821 ctcgtgcggg aacgcagcgg agttcgcctg ctcggtgccg gtctctttcg ggaagaacac
     5881 gatcaccgtc gtggcgacgg ataaccgcag gcaccgggcc gagaagaccg tgaacctgac
     5941 cgtccgtagc ggcatcccgc cgccattgat cgccgtctcg ggcagggtga cggacgccgg
     6001 cggcagtccg gttcccggcg cactggtgcg gttcgagtcg gtctttgatc tgggcggcga
     6061 acccatcgca gcaatggccg tgacggggga ggacggccgc tacctggtag aagatgccct
     6121 cggttaccgg cagacggtta cggtcgagaa ggagggttac tcccccctcc gggaggaggt
     6181 cgtcttcgag aacctgacga acaccctcga cctggagctg gagccggcga gccgtccggt
     6241 cccggggttc ggtcccgcgg taggtgtact tggtctcctt ggagcacgat ggatcctccg
     6301 gaacacgaag agacagtgaa atgatccggc agcatgccgg tgtgtctgga gaataaaccg
     6361 tgttcacctg ggaatttggg cttggcgtcg ggctaaattc atgtatgcag gggccaaatg
     6421 gtagagaccg cattgtttgt gatctccctt ttgcttttcc gggagaagga ggggccatga
     6481 gttattctga accatccaca gacaccatcc tggggataag gatgtatgag acagagcggt
     6541 tgctgttgaa ggttgcaacg cccgacgatg tcgctgcgat tgtctcgtac cttatccgga
     6601 accgtgactt tctcatggag acggaaccgc cccgggacga ccgatactat tcgttcgagt
     6661 atcaggcagg cgagatcctg cacgagcgaa agatgatgga gaatgggcag ttactcaagc
     6721 tctggattgt gaagaaggag aacccgaccc agatgatcgg caccgtcagc ttcaacacca
     6781 tcatcagagg tccgttccag tcctgcttcc ttgggtatcg tctggactat gccgaggtac
     6841 gccggggata catgaccgag gcgttgaggg aggcgatacg ggtcttgttc ggggagtgcc
     6901 gcctgcatag ggttgaagca aatatcctcc ccggcaacgg cccttctctg cgagttgtag
     6961 agaaactcgg atttcattat gagggcctcg cactccggta ccttttggtg aacggaaagt
     7021 gggaggacca tatccggatg gtgctgttaa acgacaggtg ggctggagat agatgaggtt
     7081 ccgtttgccc gcttccagcg gcatggctca tcctgtcact ccctaaactc tcttcgctat
     7141 cgtctctatc gcctcgatga tcgtccgttg ctgcggcttc atgatcgcga ccggacaatc
     7201 gacgatcttc tctacgatcg gcgcgaggat cggcgcgcag atgataccga gcgctccctc
     7261 tttgtccgcc cggacggcgg cgacgatgca ctcgtcgaga gagtccgccg agtagccccg
     7321 tatccggtat tttcggtcgc cggcctcgat atcgcggttg tcgaggtcgt cgagcaggaa
     7381 ccgcgccgcg atcaccgcga tgaagctctt ctcccggggc tcgaggacgc cgacgatccg
     7441 ccggacggtg gagaggcgcg gatcggcctt ccccgacgtg agcttgtaga gggttgcggg
     7501 cgggatctcc gcccgttccg cgagttcccg gatactcatg ttctgtcggg cgagctcgtc
     7561 ctggagtgcc cgggcaaagt cggtctcaaa gatccttctc gatagcattg ctctactata
     7621 gtatgatatt aaagataaaa tttccctttt agggtaatat ttctctcttc gcaggaaaac
     7681 ccttatcaag atccggggca actgtttctc catggacccc acacccatcc gggagatcac
     7741 gatcctcccc ggccggagcc ggagcggcga gccggaacgg ttcagggcga tcaccatccg
     7801 gcccggcgac acaatctcga tcgtcggccc caccggctcg gggaagagcg ccttcatcaa
     7861 cgacatcgag gtttttgccc agaacgacac cgcgacggga cgcacggtcc tcgtcaacgg
     7921 tgccaacccg cccgaggagt tcgtccgcga cccggcgcac aaacccgtcg ccctgatcac
     7981 ccagaacacc cggtgcctcg ccgatctcgc ggtcgcggag ttccttgcga tgcacgtccg
     8041 gtcccgaaag atcgaggacg aggatatcgt cggcaagacg atcgatcttg caaacgagtt
     8101 tacgggcgag gcgatccggc ccgatgcccg gatgaccgct ctctccggcg ggcagacccg
     8161 gtcgctcctc gtggccgatg cggtcatgat cgcggcggcg ccgatcctcc tcctcgacga
     8221 ggtggagaac gccggaatat tcaaggagcg ggtgatcgac ctcctccggg ccggggggaa
     8281 ggcggtcata ttcgtcaccc acgatcccct ggtctccctc ctctcggccc ggcggatcgt
     8341 catgcgggac ggggcggtcg agcgggtcat cgagccgggc gatcaggaga acgccgccct
     8401 ccgggaggcc atccgcctcg acggcatcct ctgccggatg cgcgacgaga tccgggccgg
     8461 caacctgatc acggaaccgg tcggggcatg aagctcgccg tcgtcgccgg gccgccgtcg
     8521 gccgggaaga ccgccgtcgt ccggcagatc atccgctccc tttcggggga gaaccggatc
     8581 gcctacatca agatcgacgt cgtccgggcg ttcgaggatg aggaactcgc ggccgagttc
     8641 ggcatcccgg cgcggaaggt ctactccggc gatctctgcc cggaccacgc cggggtgatg
     8701 gtgatgcgcg acgccatcgc ctgggccggg ggagagggcg ccgacctcct cctggtcgag
     8761 agcgccgggc tctgcctccg ctgctccccc tacacgacgc aggcgctcgg gatcgtcgtc
     8821 ctctccgcga tatcggggac gaacaccccc ctgaagatgg ccgcgatgct cgccctcgcc
     8881 gataccgcgg tcgtgacccg gatcgacctc gtctcccagg ccgagaagga ggtcttccgg
     8941 gaacgtatcc gggaggtgaa cccttacctc gatatcgtgg agacgaacgc cctccagggg
     9001 accggtatgc gctacctcct ccgggcaatc gaggagtgcc cggcgataac cgacccggat
     9061 acgatcgtcc tccggggtgc gccgccgctc ggggtctgca ccctctgcat cggaaagacc
     9121 gagatcgggt ggcagaacca cttcggggtc atccggaagc tcgagggcgc cgattacctc
     9181 taccgggggg actagaccgt ggcatggcag ccgcccggga aggactgcgg tgcctgcggc
     9241 gccccgacct gcgaggcgtt cattgccctg gtccgggagg gggagaagga ccttctggac
     9301 tgcgtcttct attctacgga tatcgcccct tcccggctcg atgctcgcca caccggccgg
     9361 gatatcctcg gtacggagta cgacttcatc ctcgaacccc tcccgggcga ggtctcggcc
     9421 cggaagatca tacttccctt ccgcccggac ctcgtggaga agtgggagat cgtgcccggg
     9481 gatatcgtcg tcgggcggcc gacgggtgcg ggctgcccgg tccagcacgt cctctcggtc
     9541 atccgtgcga atcccgtgac cggccttctc acctgcctgg tcgtcggccc gcgtgcctcg
     9601 cggggcggag ggtttaagga gatcgaggca taccacatcg tcggtttcga ggggattagc
     9661 cggacggtgc ggcgggagcc caccttcggg atgcggcagc ggttcctgcc cggctcctgc
     9721 atgatgaacc tcgcccatac aggtgtcgtg aacatggtcc tcgcacaatc ctctggtctc
     9781 catgtgcggg tggaggatat ccggctatga agacaataaa tgagatcgaa gagaagatcc
     9841 ggaacggttc tgcggtcgtc tacaccgccg cggagttcaa gcgcctcatc cgggagggtg
     9901 cggaggtcac ggccgccgat gtggatgtgg tcaccacggg aacctgtggg gtgatgtcgg
     9961 ggaccgcggc ggtcctctcg gtcccggtcg caaagcccgg gacgtttgaa cgggcggaac
    10021 ggatctgggc gaacggtgtc ccctgcatgc ccggcccctg cccgaacgag cgcctcggta
    10081 tcgtcgacat gatcgtctcc gggaccgccc atgccggcgc cggctacggg ggcggccacc
    10141 tcttccgcga tatcgtcgag gggcgcggta tcgaggtggt ggtggaggcc gccgaccgct
    10201 cggtcgaggc aacggtgacc ctcgatgact ttgcctacgc ccggctcttc accacccgga
    10261 ccgcctataa gaattacacc gcctacgtca accgccagcc gacccggata aagacgatct
    10321 tctcgatcga gggactccag ggcccctgcc gggaggcctc ggtcagcggg tgcggcgaga
    10381 tcaatccgct cgagaacgat cccctgggcc ttgctatcgg tgccgggacg ccggtcctcc
    10441 tgaacggctc ggtcggtatg gtgaccggcg aggggacccg gagcacctcc gaacgcccca
    10501 acctcacggt catcgccgac atgaccggta tgcagccccg ctacatgggc gggttcaaga
    10561 catccgccgg gccggagtgt ataacaagcc tcggcgccgc gatcccggtc ctcgacgacc
    10621 ggcagatcgc tggactcctc gtcctcgacg aggggatccc cctcccggtc gccgacatca
    10681 ccacccgcac cgtcctcggg gaagggacgt acgccgatgt ctggcagcag cccgaccgtg
    10741 aggtgaccta ccaccccggg tggtgcgaag agtgctcgac ctgtgccgcg gccgccgtct
    10801 gcccgacggg agcgttctcc cgcgaggccg gcatcgaccg cgaccgctgc ctcgcctgca
    10861 ccgcctgtct cttcgcctgc ccaaacgacg ccttcgaggc cggcgagggc tcgctccggg
    10921 tcagggggcg gcggatcccg atcacgctcc gccagtcggg ccggacactc gccgaagacc
    10981 tctgccggga cttaaaagag atgatcctcg attcccggtt cacctttacc gggggcggta
    11041 tccggtgagg gagaagtacc tcctctcctg cccggggtgc ggccggacgt tttccgaccg
    11101 ctacaccctc gactgtccat cggggtgcga cgccctcctc cggacggttt atgcggagcg
    11161 ccggcttatc ccccgggatc ttcccgggat cttccgctac aaggactggc tccccatcga
    11221 gggccacctc gggatcgatg ccggagcggt ctcctacgag agtgtcgcct ttgcccggga
    11281 attagggttg tctcacctca ccgtcacctt ctcgggctac tggcccgagc ggggcgggcg
    11341 gatggagacc tgctcgttca aggaactcga ggcgcaaccg acggttcttc gcctccggga
    11401 gaagggtgcg ggggttctcc aggtctcgtc ggcggggaac accggccggg cattctgcca
    11461 ggtatcgggc ctgaccgggg ccccggtcgt ggtggtggtt ccggcggccg ccgccggccg
    11521 gctctggacg accgtcccgg ccgagaacgt ctgtctcata acggtcgacg gggactactc
    11581 ggactccata gcgttcgggc gggaggtctg cacgctgccc gggatcgtcc ccgagggcgg
    11641 ggcaaaaaac gtcgcccgcc gggacggcat ggggacggtt atgcttgatg ggacgcttgc
    11701 cgccggcagg ctccccgatg cctacttcca ggcggtcggg agcgggaccg ggggtatcgc
    11761 cgcgtgggag gccgcagagc ggctcatcgc cgatggccgg ttcggttccc gcctcccggc
    11821 cctccacctc tcgcagaacc tccccttcgt cccgatggtc cgggcctggg aggcagggag
    11881 gcgagagatc gcggctgagg atatgcctga ggccggggcc tcgatcgccc gggtctccgc
    11941 cgatgtcctg acgaatcgtc acccgccctg gggtgtccgg ggcggagtct acgacgcgct
    12001 cgcggcatcc ggggggagga tgtacgcggt ctctaacgac gatgcccgat ccgccggccg
    12061 tctctttgag gaggcggagg agatcgacct cgacccggcg gcggcggtcg cggtcgcgtc
    12121 gctcgtccgg gcggtggagg aggggttcgt cgaaccggat gagcacatcc tcctgaacgt
    12181 gaccggcggg ggatatcaac gtgcggcaga ggacctcgac cgctacccgg tcgagccttt
    12241 cgtccgcgta gcggccggtg aggccttcga gggggatatc cgggatgccg tcagggcctg
    12301 gctcgcggag caggaggtgg ccatccgtgc gtgaaggctg tgtcctcgac cggcttgccg
    12361 ccctcggcat cgataccgtg gtgagtctcc cctgcgaccg gacgcgagaa ctctgcgccc
    12421 tcatcccgga gcggttccgt gcggtcgacc ttacccggga ggaggacggc gtcgggatct
    12481 ctgccgggct tgcgatggcg ggggcacgat cggtgctcca tatgcagagt tcggggctcg
    12541 ggaactcctt aaacgcgatc atgtcgctca ccgtcacgtt cggtctcccc ctccccatcc
    12601 tcgcga
//
LOCUS       DAOSII010000054        12444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_5100_length_12444_cov_49.222294, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000054 DAOSII010000000
VERSION     DAOSII010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 12444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12444
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5100_length_12444_cov_49.222294"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..586
                     /locus_tag="PLV90_05505"
     CDS             128..586
                     /locus_tag="PLV90_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOF96606.1"
                     /translation="MARMYARRRGTSSSVRPYRKEAPEWSNTDVAEIEKIIVNLRKDG
                     MSSSQIGLALRDRYGVPDVKLATGKRVTEILREKGLDSEIPEDLRNLMQKALGIRKHL
                     SENKKDIHNTRQLQITESKVRRLVKYYVRSGRMPKGWTYKPETAEILLSR"
     gene            595..1812
                     /locus_tag="PLV90_05510"
     CDS             595..1812
                     /locus_tag="PLV90_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOF96607.1"
                     /translation="MSLDAAAAGLADHLLEQEFVEVLAHHDADGIAAASILCHAMFRK
                     GGRFRLRIRPGITAADVPEEGSVLLCDFGSTLQDLPGDVMVVDHHLPHFEGDHHVNPR
                     LAGIDGDRNLSAAGTAYIVAQRMGDNRDLAGLALLGILGDGQDIEGPNRDIVNEGIAN
                     GFITPRRGLRLPGRGLAEQLALAVSPYLTGLSGEPDAARALVAGVTDEDDVDIEALLS
                     RVVLTAAPKASHTALCGIWGTTYSLGREVIDEAGNLAAVVDACGKAGCGDIGASLCLR
                     STHALQEAWDVTVRHRRAVVAGIRGASRLDDRLALFTVDDGTVTGDVADALARDFPAT
                     GPVFVIGVSGDGYSVSARCPPDVDLDLDALMRRLAGACGGRGGGHHRRAGAHIGADQV
                     DRFRQGLREAMPA"
     gene            1809..2066
                     /locus_tag="PLV90_05515"
     CDS             1809..2066
                     /locus_tag="PLV90_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HOF96608.1"
                     /translation="MTRIEGTIETPHSLPGCVAAALEPDNLTLIRTYPVRGGVRAEIG
                     GTRLRSIIASVDDYLMNLAIAEEICNLPVRSERAAQNKDIE"
     gene            2080..2673
                     /locus_tag="PLV90_05520"
     CDS             2080..2673
                     /locus_tag="PLV90_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3ae"
                     /protein_id="HOF96609.1"
                     /translation="MARKKQVGRRVEGWKAKKWYRVYVPETFGKVEIGDTISADPANM
                     VGRVMTATLGEVVQDYSKSHIKMRFKINNVAGDAAYTEFVGHEVTRDYLRSMVKRRAS
                     RIDTIHPIVSKDGKLLRVTVVCLTISRAEQSQVHAVRQAISQALSARAAESDFETLVK
                     EIVSGDMARDVFKVVKTIYPIRRVEITKSKLEQIAVV"
     gene            2757..3197
                     /locus_tag="PLV90_05525"
     CDS             2757..3197
                     /locus_tag="PLV90_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96610.1"
                     /translation="MTGYTSAGGTVTAEEEHTDARQVREMALCPGPPEERQKMAFGII
                     RRMLVQFHGEWTALREKESDDAWLDAAHALLDRYAHQLHDVVCDVQVIVGDDIVIDIR
                     CLSADMITTTNILVMIGCSEECCRRGDALAKEALRHAERCLSLR"
     gene            3238..4473
                     /locus_tag="PLV90_05530"
     CDS             3238..4473
                     /locus_tag="PLV90_05530"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOF96611.1"
                     /translation="MIAQKVLFLVLDGISDRPCDVLDGRTPLAAARTPVLDRLAAEGV
                     CGIMDSIAPGIRPGSDTSHLALLGYPPQEYYTGRGPLEAEGTGIRMTAGMIGFRCNFA
                     ATDAEGRVTDRRAGRISGTAPLAEAIRDGVDLSPLGVGFRFESGAGHRAALALAGSGL
                     GDKVSSNDPKRDGVRPPAIRPCTDDPEDRKTAEACNEFIRQSHDILSRHPMNVRRMEE
                     GLAPGNLLLIRGAGKMGAFPQFSSRYGLSGSVISAATLIAGIGKVVGLEHIPVPGTTG
                     SVDSDLDAKVKAALAELDRKDFVLLNIKGADEAGHDGRGIQKRDFIEVIDAALEPLLD
                     LGDTLLVVCADHSTPCSIKDHSADPVPVVIRGPGVRTDRVARFDEVSCAEGGLHRIRG
                     RDLMPIALDLINKSHKYGA"
     gene            4490..>5307
                     /locus_tag="PLV90_05535"
     CDS             4490..>5307
                     /locus_tag="PLV90_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOF96612.1"
                     /translation="MEQTGDHDWIKGCTLPDHTELQERTLKTAQDIVIGDRCRIDYGL
                     AGNDVVVCEFSKINGTIVAGGDVRIDNWCEVGGDVIAEEDAYLGEGVKIRGKLVVRGD
                     LDIGDNVQIERGFEAKGWISIRNPMPVIIYIMMYLVAVLGIEKQEDLDAVLQKLFGDD
                     EVRTEAPLMIPAGAVLNMQTFSVPERMAVGSGCRLHGNIRAASIAIREETTIFGSLHA
                     REGAKVARRSVIHGDVQSDGDVVIEQEAHILGNVSCRGLTLHEDARVDGVIRAPG"
     assembly_gap    5308..5407
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5408..5969
                     /locus_tag="PLV90_05540"
     CDS             <5408..5969
                     /locus_tag="PLV90_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96613.1"
                     /translation="WLAAVREYYSLDILKTITPALEDLRPDREEKIRDLLDEVWGNRA
                     GALCLDCCCGSGVGTAALRANGVQALAYDNDSSLLALGLSRGRLVPEDTMYIDAREAF
                     RYIAPVPLGVAFMAGEIYSYNADLWRSIIAELLALTDEALITVGTEAEATRVEGWCAG
                     EGWDTRVFENPRDPIYDRWCCVARRG"
     gene            6079..7272
                     /locus_tag="PLV90_05545"
     CDS             6079..7272
                     /locus_tag="PLV90_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF96614.1"
                     /translation="MNTARRIYNVLFISVFATMLGLGIVSPLLPIYAENLGATGIWLG
                     VIFSAFALSRSVFMPIIGRISDRRGRKWIILTGMFAYAVLSLAYLIAGSVYSLTAVRF
                     AHGLASAMVVPIAMAYIADLSEKGKEGSHMGNFSISMFLGMGVGPLLGGFLNDTFGMP
                     SVFYAMAGLSAFATLLVAISLPEAKPGGFALPDAPSTPMPALITLPVMQGVMVFAFIS
                     ALGRGGMMVFVPVFGPLIAISPFEVGIVLSANTFLMALLQVPIGRLTDTGNKVALIVA
                     GSTIAAIALAVIPLTHSFLPLLLVTSLIGVGGAIQQPAIMALTVDAGRTIGMGTSMGA
                     YNTVFGIGMIIAPLMGGAFMDFVGIEAVFYVGGAISLIGTVIFAAMMQRSARREVAGE
                     TPGPG"
     gene            complement(7321..>7836)
                     /locus_tag="PLV90_05550"
     CDS             complement(7321..>7836)
                     /locus_tag="PLV90_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOF96615.1"
                     /translation="DALAMGIQWLTVQEFHQMMGRAGRPDFHDLGRVVILAEPGGSYS
                     RTSGKTEEEVAVGLLRGEMEEVAPEYDMEQSSEEFVANAVVCGGDEQQVIRMTRSMVG
                     TLEPALPTLLDYGLVRRRAGRIELTDMARVMAEHFIGAERLIEIKDLVRRMDDAAEIV
                     AELECAEEKTP"
     assembly_gap    7837..7936
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7937..9196)
                     /locus_tag="PLV90_05555"
     CDS             complement(<7937..9196)
                     /locus_tag="PLV90_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOF96616.1"
                     /translation="MMVIVQPQKGTYKLLFVEGGRVEGSGYVDLTATPKGQRPKNFKM
                     RRRGKQLKPTPTRDLITLLRRSSVHLAGRSPEFESFLADLQIHSSRIDICRYCQLEDR
                     FVPVDKATGVRYGGEWICMECAKREMRRELGYMGKFGGSVLVHLEKLLAQVRDLDRVL
                     ASVGPEKPDRSRTLFDRLEAHEVQKTAHITDLALPPAFAKAAGVEYLMPVQQLAVQSG
                     LLEGQHLLVVSATASGKTFIGEMAGMKNFLEGRGRMLFLVPLVALANQKYQRFRDRYG
                     HLVRVALQTGVSRLNLPETRPAGERDIGAPVVVGTYEGIDHALRTGRPMKGIGTVVID
                     EVQMLEDPDRGHRLDGLIARLKQIAPDAQFLYLSATIGMPGLLAEKLRANLVRYADRP
                     VPLERHLLFIERAKKIGFIKQMVAEEYK"
     gene            complement(9193..10002)
                     /locus_tag="PLV90_05560"
     CDS             complement(9193..10002)
                     /locus_tag="PLV90_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOF96617.1"
                     /translation="MTDYTDDPLYVILRSKREATRFQILVEIAEHQPSIRQQEIAGKL
                     GVTPQAISEYIREMVDEGLVTAHGRGRYEVTKSGIEWVLRHAEALESYARHINRDIIQ
                     QVAVWTAIAREDIRKGDTVGVLMQDGWLYATKQEQNATGRATMDAKPGEDLGVAQLSG
                     LIEHEEGLIHVCKVPRVERGGSRKVSPDLLRAAVRDAEMVAAVGLESYVALRKADIEP
                     DMFFGSREGVIEAAFHGRECAILIVDEEFTDFLKRLEATGLAYTIHDLLAS"
     gene            complement(9986..10498)
                     /locus_tag="PLV90_05565"
     CDS             complement(9986..10498)
                     /locus_tag="PLV90_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HOF96618.1"
                     /translation="MTGHIKILNDPVELVPLLITFNNADYKRIYELLNKTWLTEEELG
                     AHADTSTVIACLSILRKGNLVEEQWRMPKPGEKPTKEYRATYSKFRASFQCSMGDIGD
                     LLHIAISNDAGLRSIVDSVEQEIVAGTTSIGDISRKYGVSPIFIRGLAKRIPGLDVKG
                     QGMVLLDRLH"
     gene            10556..>12444
                     /gene="hypF"
                     /locus_tag="PLV90_05570"
     CDS             10556..>12444
                     /gene="hypF"
                     /locus_tag="PLV90_05570"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOF96619.1"
                     /translation="MRTQGTIIIRGIVQGVGFRPFVYAQAQKFDITGTVKNLGSEVEI
                     HAFGDRFEEFLGAVSRGPPLSRIDSVEVRDLRGNRPEEFVILESSSGSLSGFIPADVA
                     ICDDCVGDILTPGGRYEGYWATSCVNCGPRYSIITAIPYDRERTAMEAFPMCAACESE
                     YTDPSCRRHHAQTIACAACGPRLSLLRSDGTPVLGDPIREAAALLDAGFIVAVRGIGG
                     FHIACIEEAAGELKRRLGRTEQPLAVMATAGEVERIAVVSDEDRRILHSRERPIVVLA
                     KRDPASHAGISNLHTIGCMLPYTGLHHLLFAHLAHPLLIMTSANLPGYPMITDLEIAL
                     GRLSGEVDYILTHDRRIVNRCDDSVVRDGYIIRLSRGLAPKRAAIDLGDACILGVGPE
                     LNANISVYRNGFCITSPHVGNVRNPETLAYLQETAEKIGGLVGARYDTVVHDLHPQFL
                     STRYAREVSEATGAELLPVQHHRAHIAAVTRDECVGIAIDGVGYGDDGTVWGGEVFAG
                     RVPHLDRVAHLEVVPMPGGDLATRFPERMLYGILPTDSIRDLLSSRGWSDMELAVLAR
                     QVERGLNVAATSSTGRVLDAAAALLGICRERTYDGEPAMKLESAAYAGRPAAWEIEFS
                     RDGGC"
BASE COUNT         2139 a         4006 c         3939 g         2160 t
ORIGIN      
        1 atgtgctggc tatggtaagt ccgacgtgct cccggaagca ctgcccgcaa gggctcggga
       61 ttacagagat tagtcagtca acggactttt ttctggactt aacatagtgt atatcggagg
      121 tgtatgaatg gcaagaatgt acgcccggcg ccgtggaacc agcagttccg tccggcccta
      181 ccggaaggag gctcccgagt ggtccaacac ggacgtagcg gagatcgaga agatcatcgt
      241 caatctccgc aaagacggca tgtcgagcag tcagataggt cttgcgctgc gggaccggta
      301 cggcgtccct gacgtcaagc tcgccaccgg caagcgtgta accgagatcc tccgcgagaa
      361 gggccttgat tccgagatcc ccgaggatct ccggaacctg atgcagaagg cgctcggtat
      421 aagaaagcac ctttctgaga acaaaaagga catccacaac actcgccagc tccagatcac
      481 cgagtccaag gtgcgcaggc tggtaaagta ctacgtcagg tccggacgga tgccgaaagg
      541 ctggacttac aaaccggaga ctgcagagat cctgctctcc cgctgagacg atccatgtcg
      601 ctggacgctg ctgccgccgg tctcgccgac catctcctcg agcaggagtt cgtggaagtg
      661 ctcgcgcacc acgatgcgga cggtatcgcc gccgcatcca tcctctgcca cgccatgttc
      721 cgcaagggcg gacggttccg gctgaggatt cgcccgggca tcaccgcggc cgacgtgccg
      781 gaggagggca gcgtgctcct ctgcgacttc ggatcgacac tccaggacct ccccggcgac
      841 gtgatggtgg tagaccacca cctgccccac ttcgagggcg accaccacgt caacccgcgt
      901 ctcgccggga tcgacggcga ccggaacctc tccgcggccg gcacggccta catcgtcgcg
      961 cagcgcatgg gggacaaccg cgaccttgcg ggcctcgcgc tcctcggcat actcggcgac
     1021 ggccaggata tcgaggggcc gaaccgggat atcgtgaacg aaggcatagc aaacgggttc
     1081 atcacgcccc gccgcggcct tcgcctgccc gggcgaggtc tcgccgagca gctcgctctc
     1141 gccgtcagcc cctacctcac cgggctctcg ggcgaacccg acgccgcccg ggccctggtt
     1201 gccggggtca ccgacgagga cgacgtggat atcgaggccc tcctctcccg ggtcgttctt
     1261 acggccgccc cgaaagcgtc gcataccgcg ctctgcggca tatggggcac cacctacagc
     1321 ctcgggcgag aggtgatcga cgaggcgggg aaccttgccg ccgtcgtcga tgcctgcggc
     1381 aaggccgggt gcggggatat cggtgcatcg ctctgcctcc gctcgaccca cgctctccag
     1441 gaggcctggg atgtaaccgt ccgccaccgg cgcgcggtcg tcgccggcat ccgcggggca
     1501 agccgcctcg acgatcgtct cgccctcttc acggtggacg acgggacggt gacgggcgac
     1561 gtcgcggatg cactcgcgcg cgatttccct gcaaccggcc ccgtcttcgt catcggggta
     1621 agcggggacg gctactccgt atcggcccgc tgcccgccgg acgtcgacct cgacctcgat
     1681 gcgctgatgc ggaggctcgc aggggcgtgc ggcggccggg gcggcggaca ccaccggagg
     1741 gccggcgccc atatcggggc cgaccaggtg gaccggttca ggcagggact gcgcgaggcg
     1801 atgcccgcat gacccggatc gaggggacca tcgagacgcc gcacagcctt cccggttgcg
     1861 tggcggcggc gctcgagccc gacaacctca ccctgatcag gacgtacccg gtcaggggag
     1921 gggtgcgggc cgagatcggc gggaccaggc tccggtcgat catagcatcg gtggacgact
     1981 acctcatgaa cctcgcgatc gcggaggaga tctgtaacct ccccgtccgg agtgaacgtg
     2041 cggctcagaa caaggacata gagtaagtga agtgatacca tggcaaggaa gaaacaggtt
     2101 ggaagaagag tagaaggctg gaaggccaag aagtggtacc gcgtctacgt acccgagacc
     2161 ttcggcaaag tcgagatcgg cgataccata tcggccgacc ccgcgaacat ggtcggccgg
     2221 gtcatgaccg cgacgctcgg cgaagtggtg caggactact ccaagtccca catcaagatg
     2281 agattcaaga tcaataacgt ggccggcgac gccgcgtata ccgagttcgt cgggcacgag
     2341 gtgacccggg actacctccg gtcgatggtc aaacggcggg cgtcccgtat cgacaccatc
     2401 cacccgatcg tcagcaagga cggtaaactc ctgcgcgtga cggtcgtctg tctcaccata
     2461 tcgagggccg aacagagcca ggtccacgcc gtgcgccagg cgatatcgca ggccctctcc
     2521 gccagggcag cggagagcga ctttgagacc ctggtcaagg agatcgtctc cggcgacatg
     2581 gcccgcgacg tcttcaaggt cgtcaagacg atctacccca tccgccgggt cgagatcacc
     2641 aaatccaaac tcgagcagat cgcggtcgta taaggccggc cgctcaccct tatttttagc
     2701 cgcctgcatc cgtagtgttc gcgggcgacg gagatgccgc gaagggtata taccctatga
     2761 ccgggtacac ctcagccgga gggacggtga cggccgagga agagcatacg gatgcgcggc
     2821 aggtaagaga gatggcgctc tgcccgggac ccccggagga gcggcagaag atggccttcg
     2881 ggatcatcag gaggatgctc gtccagttcc atggggagtg gaccgccctc cgcgaaaagg
     2941 agagcgacga cgcctggctc gacgccgcgc acgcgctgct cgaccggtat gcgcaccagc
     3001 tccacgacgt cgtctgcgac gtgcaggtga tcgtcggcga cgacatcgtc atcgatatcc
     3061 ggtgcctctc ggccgacatg atcacgacga cgaacatcct cgtcatgatc ggttgcagcg
     3121 aggagtgctg caggcggggc gacgccctcg caaaggaggc cctccgccac gcggagaggt
     3181 gtctatcgct ccggtgaccg ggaagagtaa tcaggttccg gatcgcacca gtctctgatg
     3241 attgctcaga aggtgctctt cctcgtactg gacggtatat ccgaccgtcc ctgcgatgtg
     3301 ctggatggac ggacccctct tgccgccgca cggaccccgg tcctcgaccg gctcgccgcg
     3361 gagggcgtct gcggcatcat ggactccatc gctcccggca tacgcccggg ctccgacacc
     3421 tcgcacctcg ccctcctcgg ctacccgccg caggagtact acaccggccg cggcccgctc
     3481 gaggccgagg ggaccggcat ccgcatgacc gccggtatga tcggtttccg gtgcaacttc
     3541 gccgcgaccg acgcggaggg ccgcgtcacc gaccgcaggg ccgggcggat atccgggaca
     3601 gcaccgctcg cggaggcgat ccgggacggc gtcgacctct cgcctctcgg cgtcgggttc
     3661 aggttcgagt cgggcgccgg ccaccgggcc gccctcgctc tcgcaggaag tgggcttggc
     3721 gataaggtct cctcgaacga cccgaagcgc gatggggtgc ggccgcccgc catccggccc
     3781 tgcaccgacg atccggaaga tagaaagacg gcggaggcct gcaacgagtt catccgccag
     3841 tcccatgata tcctctctcg ccacccgatg aacgtccggc ggatggaaga ggggctcgcg
     3901 ccgggaaacc tcctcctgat ccggggcgcc ggaaagatgg gagcgttccc gcagttctcc
     3961 agtcgttacg gtctttccgg gagcgtcata tccgcggcca ccctcatcgc cggcatcggg
     4021 aaggtcgtgg ggctcgagca catccccgtg ccggggacga ccgggtcggt cgactccgac
     4081 ctcgacgcca aggtgaaggc ggcgcttgcg gagctcgacc gcaaagactt cgttctcttg
     4141 aacatcaagg gcgcggacga ggccggccac gacgggaggg gcatccagaa gcgggacttc
     4201 atcgaggtga tcgatgcggc gctcgaaccg ctcctcgatc tcggggatac cctgctcgtc
     4261 gtctgcgccg accacagcac cccctgctcg atcaaggatc acagcgccga cccggtgccg
     4321 gtcgtcatca ggggtccggg ggtccggacg gatcgggtag cccggtttga tgaggtctcg
     4381 tgcgcagagg gcggccttca ccgcatccgc ggccgcgatc tcatgccaat cgccctcgat
     4441 ctaattaata agagtcataa gtatggcgca tgaagatcat accgaataaa tggaacagac
     4501 tggcgaccac gactggatca agggctgcac cctcccggac cacaccgagc tccaggagag
     4561 gacgctcaag accgcgcagg atatcgtcat cggtgaccgg tgccggatcg attacggcct
     4621 tgcgggaaac gacgtcgtgg tctgtgaatt cagcaagatc aacggcacca tcgtcgcggg
     4681 cggcgacgtc cggatagaca actggtgcga ggtcggcggc gacgtcatcg cagaggagga
     4741 cgcctacctc ggcgaggggg tgaagatccg gggcaagctc gtcgtcagag gcgacctcga
     4801 tatcggagac aacgtccaga tcgagcgcgg gttcgaggcg aaaggctgga tatccatccg
     4861 taacccgatg ccggtcatca tctacatcat gatgtacctc gtggccgtcc tcggtatcga
     4921 gaagcaggag gacctggatg ccgtcctcca gaaactcttc ggcgacgacg aggtccgcac
     4981 ggaggcgccg ctgatgatcc cggccggcgc cgtcctcaac atgcagacct tctcggtgcc
     5041 tgagaggatg gccgtcgggt cggggtgccg gctgcacggg aacatccgtg cggcatcgat
     5101 cgccatacga gaagagacga cgatctttgg gagcctgcac gcacgggaag gcgcgaaagt
     5161 cgctcgcaga tcggtcatcc acggcgacgt ccagagcgac ggcgacgtcg tcatcgagca
     5221 ggaggcacat atactcggga acgtctcctg ccggggcctc accctgcacg aggatgcccg
     5281 cgtggacggg gtcatccggg cgccgggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnctg gctcgcggcg gtccgcgagt actacagcct ggatattctc aagaccatca
     5461 cccctgctct cgaagacctg cgccccgacc gtgaagagaa gatccgcgac ctcctcgatg
     5521 aggtctgggg caatcgcgcg ggagccctct gcctcgactg ctgctgcggg tcgggcgtcg
     5581 gcaccgcggc actccgggcg aacggcgtgc aggcgctcgc ctacgacaac gattcgtcgc
     5641 ttcttgcact cggcctatcc aggggacgcc tcgtgccgga ggataccatg tacatcgacg
     5701 cgcgggaggc cttccgctac atcgcgccgg tgcccctcgg cgttgcgttc atggccgggg
     5761 agatctactc ctacaacgcc gacctctgga gatcgatcat cgccgagctc cttgccctca
     5821 cggacgaggc cctgatcacc gtcggcacag aggccgaggc cacccgggtg gaggggtggt
     5881 gcgccggcga ggggtgggat acccgggtct ttgagaatcc gcgcgacccc atatacgacc
     5941 ggtggtgctg cgtcgcgcgg cgggggtgaa ccccggccac cgggctaata tatatggtaa
     6001 attatatccc actctttctg ctgcatttgc aaacgatcga tcccatgcgg atctcacctc
     6061 caaccggatg ccatccccat gaataccgcc agaagaatct ataacgtcct cttcatatcc
     6121 gtctttgcca cgatgctcgg tctcggcatc gtcagcccgc tcctcccgat ctacgcggag
     6181 aacctcggag cgaccggcat ctggctcggc gtcatctttt cagccttcgc tctctcccgg
     6241 tcggtcttca tgcccatcat cggccggata tcggataggc gggggaggaa gtggatcatc
     6301 ctcaccggca tgttcgccta cgcggtcctc tcgcttgcct acctcatcgc cggcagcgtc
     6361 tattcgctca cggccgtccg cttcgcccac ggcctggcgt cggccatggt cgtcccgata
     6421 gccatggcct acattgccga cctctcggag aagggaaaag aagggagcca catggggaac
     6481 ttctccatat cgatgttcct cggtatgggg gtggggccgc tcctcggagg gttcttaaac
     6541 gacacgttcg gcatgccgtc ggtcttctac gccatggccg gcctctcggc gtttgccacg
     6601 ctcctcgtcg ccatatccct cccggaggca aaaccggggg gttttgctct cccggatgcc
     6661 ccgagcaccc cgatgcccgc actcatcacg ctgccggtca tgcagggggt catggtcttt
     6721 gccttcatca gcgccctcgg tcgcgggggt atgatggtct tcgtcccggt cttcggcccg
     6781 ctgatcgcca taagcccgtt cgaggtaggg atagtcctct ccgcaaacac cttcctgatg
     6841 gcgctcctcc aggtgcctat cgggaggctc acggataccg ggaacaaggt cgccctgatc
     6901 gtcgcggggt cgaccatagc ggcgatcgcg cttgccgtga tccccctgac ccattcgttc
     6961 ctgcccctgc tcctggtcac ctccctcata ggggtcggag gcgccatcca gcagccggcc
     7021 atcatggccc tgacggtcga tgcgggtcgg acgatcggta tggggacatc gatgggcgcc
     7081 tataataccg tgttcggcat cggcatgatc atcgcgccgc tgatgggagg cgcgttcatg
     7141 gacttcgtcg gtatcgaggc cgtcttctac gtgggcggcg cgatcagcct catcgggacc
     7201 gtcatatttg ccgcgatgat gcagaggagc gcgcgccggg aagtcgccgg agagacaccc
     7261 gggcccgggt aggcactctt tgcagcccca aagcgtcccg gcagtacgaa agatgcactt
     7321 ttacggcgtc ttctcctcgg cacactcgag ttccgcgacg atctcggccg cgtcgtccat
     7381 ccgccggacc agatccttga tctcgatcag gcgttcggcg ccgatgaagt gctcggccat
     7441 cacccgggcc atatcggtga gctcgatgcg tcctgcccgc cgccggacca gcccgtaatc
     7501 gaggagggtg gggagcgccg gctccagcgt cccgaccatc gagcgggtca tgcggatgac
     7561 ctgctgctcg tcgccgccgc agacgacggc gtttgcgacg aactcctcgg aactctgctc
     7621 catgtcgtac tcgggggcca cctcctccat ctcgccccgg aggaggccga cggcgacctc
     7681 ttcctcggtc ttcccggatg tccgggagta cgacccgccg ggctccgcga gtatgaccac
     7741 ccggccgagg tcgtggaagt cgggccggcc ggcccggccc atcatctggt ggaactcctg
     7801 gacggtcagc cactggatgc ccatcgcgag agcgtcnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnncttg tactcctcgg cgaccatctg tttgatgaac ccgatcttct
     7981 ttgcacgttc gatgaagagg aggtggcgct ccagcggcac cggcctgtcc gcgtagcgaa
     8041 caaggtttgc ccggagtttc tccgcaagca gccccggcat gccgatcgtg gccgagaggt
     8101 agaggaactg ggcatcgggg gcgatctgct tgaggcgggc gatcagcccg tcgaggcggt
     8161 ggccccggtc ggggtcctcg agcatctgga cctcgtcgat gacgacggtc ccgatgcctt
     8221 tcatgggccg cccggtgcgg agggcgtgat cgatcccctc gtaggtcccc accacgacgg
     8281 gggcgccgat gtcccgctcc ccggccggac gggtctcggg gaggttgagg cggctcaccc
     8341 cggtctggag ggcgacccgc accaggtggc cgtaacggtc gcggaaccgc tgatacttct
     8401 ggtttgcgag cgcgaccagc gggaccagga agagcatcct cccccgccct tcgaggaagt
     8461 tcttcatgcc ggccatctcc ccgatgaacg tcttgccgct cgcggtggcg gagacgacga
     8521 ggaggtgctg cccctcgagg agcccgctct ggacggcgag ctgctggacg ggcatcaggt
     8581 actcgacgcc ggcggccttt gcaaacgcgg ggggcagggc aaggtcggtg atatgggcgg
     8641 tcttctggac ctcgtgcgcc tcgagccggt caaagagggt ccgcgaccgg tcgggcttct
     8701 ccggccccac cgacgcgagc acccggtcga ggtcgcggac ctgggcaagg agtttctcga
     8761 gatggacgag cacggatccg ccgaacttac ccatgtagcc gagttcccgg cgcatctcgc
     8821 gttttgcaca ctccatgcag atccactctc cgccgtaccg gacaccggtc gccttatcga
     8881 cggggacgaa ccggtcctca agctggcagt agcggcagat atcgatccgg gacgaatgga
     8941 tctggaggtc ggcaaggaac gactcgaact ccgggctcct ccccgcaagg tggaccgaag
     9001 accggcgcag gagcgttatg aggtcacggg tcggggtggg cttgagctgt tttccccgcc
     9061 ggcgcatctt gaagttcttg gggcgctggc ctttcggcgt cgcggtcagg tcgacgtacc
     9121 cggaaccctc gacgcgcccg ccttcgacga ataaaagttt atacgtgcct ttctggggct
     9181 ggacgatgac catcatgatg cgagcaggtc atggatcgtg taggcaagcc ccgtcgcctc
     9241 gagccgtttg aggaagtcgg taaactcctc gtcgacgatg agtatcgcgc actcccggcc
     9301 gtggaacgcc gcctcgatga ccccttcccg cgagccaaag aacatatcgg gctcgatatc
     9361 cgccttcctg agggccacgt aggactcgag gccgacggcg gccaccatct ccgcatcccg
     9421 gacggcggcc cggaggagat cgggcgagac cttccgcgac ccgccccgct ccacccgcgg
     9481 caccttgcag acgtggatga gcccctcctc gtgctcgatg aggccgctca actgggcgac
     9541 gccgaggtcc tccccgggtt ttgcatccat cgtcgccctg cccgtggcgt tctgctcctg
     9601 cttcgtcgca tagagccacc cgtcctgcat caggacgccg accgtatccc ctttccgtat
     9661 atcctcccgg gcgatggccg tccagacggc gacctgctgg atgatgtcgc ggttgatgtg
     9721 gcgggcatag gactcgagcg cctcggcgtg ccgcaggacc cactcgatgc cgctcttggt
     9781 cacctcatac cgcccccggc cgtgagcggt gacgagcccc tcgtccacca tctccctgat
     9841 gtactcggag atcgcctgcg gcgtcacgcc gagtttcccg gcgatctcct gctggcgaat
     9901 ggatggctgg tgctcggcga tctccacgag gatctggaac cgggtggctt cgcgcttgct
     9961 gcgcaggatg acgtagagcg gatcgtcagt gtagtcggtc aagcagcacc attccctgcc
    10021 ctttcacatc gagaccgggg atcctctttg caagccccct gatgaagatc gggctgaccc
    10081 cgtactttcg ggatatatcg ccgatcgatg tggtcccggc tacgatctcc tgttcgaccg
    10141 agtctacgat cgagcggagg cctgcatcgt tggatatggc gatgtgcaga aggtccccga
    10201 tatcgcccat ggaacattga aaactggccc tgaatttgct gtatgtcgcc cggtattcct
    10261 tcgtcggttt ctctccgggt ttcggcatcc gccactgctc ctcgaccagg ttgcccttcc
    10321 tgaggatgga gaggcacgcg atcacggtcg aggtatcggc gtgggccccc agttcttctt
    10381 cggtcagcca ggtcttgttt aagagctcgt agattctttt atagtctgca ttattgaacg
    10441 ttataagaag aggaaccagt tccaccggat cgttaagaat tttaatatgt cccgtcaatg
    10501 cgatacccta ttattatatc gtggtataac tccataaatt tttgctgaat tttcgatgcg
    10561 tacacagggc accatcatca tacggggcat cgtccagggg gtcggatttc gccccttcgt
    10621 ctatgcgcag gcgcagaaat tcgatatcac cgggaccgtc aagaacctcg ggagcgaggt
    10681 ggagatccat gccttcgggg accgtttcga ggaattttta ggggcggtct cccgcgggcc
    10741 gccgctatcc cgcatcgact cggtggaggt cagggatctg cgcggcaacc ggcccgaaga
    10801 gttcgtcata ctcgagagtt cctcgggaag cctctcgggg ttcattccgg ccgacgtcgc
    10861 gatctgcgac gactgcgtcg gcgatatcct cacgccgggc gggcgctacg agggatactg
    10921 ggccacgtcc tgcgtcaact gcggcccccg ctacagcatc atcaccgcca tcccctacga
    10981 ccgggaacgc accgccatgg aggcgttccc gatgtgtgcc gcctgcgaga gtgagtacac
    11041 cgacccgtcg tgccggcgcc accacgccca gacgatcgcc tgtgccgcct gcggcccccg
    11101 tctctccctc ctccggtcgg acgggacgcc ggttttgggg gatcccatcc gcgaggcggc
    11161 ggccctcctc gatgcgggtt ttatcgtcgc cgtccggggg atcggggggt tccacatcgc
    11221 ctgcatcgag gaggcggccg gcgaactgaa acgccgcctg ggccggacgg agcagcctct
    11281 cgcggtgatg gcgaccgcgg gcgaagtgga gcggatcgcg gtcgtctcgg acgaggaccg
    11341 gcggattctc cactcgcggg agcggccgat cgtggtgctc gcgaaacggg atcccgcgtc
    11401 tcacgcaggt atatcgaacc ttcacaccat cgggtgcatg ctcccctaca ccggtctgca
    11461 ccacctcctc tttgcgcacc ttgcccaccc gctcctcatc atgacgagcg cgaacctgcc
    11521 gggctacccg atgatcacgg accttgagat tgccctcggc cggctctcgg gcgaggtgga
    11581 ctacatactc acccacgacc gccggatcgt caaccggtgc gacgactcgg tcgtcaggga
    11641 cggctacatc atccgcctct cgcggggcct tgccccgaag cgggcggcga tcgatcttgg
    11701 ggatgcgtgc atcctcggcg tcggcccgga actgaacgcg aacatatcgg tctaccgtaa
    11761 cgggttctgc atcacgtcgc cccacgtggg gaacgtcagg aaccccgaga cccttgccta
    11821 cctgcaggag acggcggaga agatcggggg gctcgtcggc gcacgatacg atacggtcgt
    11881 ccacgacctc cacccgcagt tcctctcgac ccgctacgcc cgggaggtta gcgaggcgac
    11941 cggggcggag ttgctccccg tccagcacca ccgggcgcac atagccgccg tgacccgtga
    12001 tgagtgcgtc gggatagcga tcgacggcgt gggctacggc gacgacggga cggtctgggg
    12061 cggggaggtc tttgccgggc gggtcccgca cctcgaccgg gtcgcccacc tcgaggtcgt
    12121 cccgatgccg ggcggcgatc ttgccacccg gttcccggag cggatgctgt acgggatcct
    12181 gccgacggat tcgatccggg atctcctttc gtcgcggggc tggagcgata tggagctcgc
    12241 ggtcctcgcc cggcaggtgg aacgggggtt aaacgtcgcc gcgacatcga gcaccggccg
    12301 ggtgctggat gcggcggcag ccctcctcgg tatctgccgg gagcggacct acgacgggga
    12361 gccggcgatg aagcttgagt cggcggcgta tgcggggcgg ccggcggcct gggagatcga
    12421 gttctcgcgc gacggagggt gcga
//
LOCUS       DAOSII010000055        12335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_5154_length_12335_cov_42.031270, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000055 DAOSII010000000
VERSION     DAOSII010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 12335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12335
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5154_length_12335_cov_42.031270"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PLV90_05575"
     CDS             <1..625
                     /locus_tag="PLV90_05575"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOF96620.1"
                     /translation="ATTTAGNATTTNTTAGAGTTPSSSLAVTPDHGQVSQIWDLLEPP
                     AGDRTVWELLQATETAIPFEETPVYGNFTIFIDLLQTTGVNQVLQGNDSYTVFAPTDG
                     AFVELPPDDMNALTKTTGNQSGAINRTAVALNHIVPGSYTESALRNETTLVTLAGSNL
                     TVNATGNETRVGNATIIIPDLVAKNGAIQGINRVLLPADTGGAANLS"
     gene            858..3107
                     /locus_tag="PLV90_05580"
     CDS             858..3107
                     /locus_tag="PLV90_05580"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOF96621.1"
                     /translation="MTKVTLVIASLLIIALIAGVGAQQAGLSNASSTNESSNQTTQST
                     PGNVTDLTNAGDSLAATFQNNESLTNLAPIPSFTADIGLELIGENFTAPMMVTSPDDG
                     TGRLFVVDQIGVAKIVDVNGTELPEPFLDLRDNLVKLDPTYDERGLLSIAFHPNYRNN
                     GKVYAFYSAPLRPEAPEGWSCTNHISEFQVDPDNPNTVNMSSEKVLMYIDKPYENHNG
                     GVLAFGPADGYLYISVGDGGKANDVGNGHTPGIGNAQDLTKIYGKILRIDVDSTATGV
                     TIVQQNQTENQTGGASANRTENPPEPTWTTFAGSLYGIPPDNPFAENQPEILDSYAYK
                     TVPQEIYAYGFRNPAYMAFDASDGNALYVAMAGQNLFESVLIVLNGGNYGWNIREGTH
                     CFDPNAPTAPGESCNSTGLLGEPLIGPIFEGGHDLGIVIVGGDVYRGNTVVGLQGRYI
                     FGYWSDSRTVGNGTLLAATPPAGWAEGALPPTAAGLTPEDNAMWQAQIVNATAQNSDT
                     LGMFLRGFGEDADLDMYVLTSDVGGPDASTSTGKVWKIVPPTIVNPALGAGALEVTPT
                     VEAENQTENQTGTTAVGGGMGAGATPLSSLSSSDPAGNIMEVLALTETGIPFEETPAF
                     GNFTLFTGLLRSTGVAATLEGPGPYTVFAPTDDAFERMDPDIREPILNGSRDSKDIVL
                     YHIVNGSSTLADLKNVSTLRTLQGENLTINATGTVVRVENATIAIPDIMADNGVIQGI
                     DTVLIPADT"
     gene            3191..4405
                     /locus_tag="PLV90_05585"
     CDS             3191..4405
                     /locus_tag="PLV90_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOF96622.1"
                     /translation="MMYQQIFVGVDGSPCSDMAGDAALALTAGGRGRCTGCHVYAARM
                     HRQRFEDMEPGLPGQYQEEERLDDLRATHDDLISGGMALISDAYLAPLAEKAAAQGIP
                     FEPVIAEGRGYVEVLRLIRERRPDLAVLGATGHGQTPEVPLGGLAERVLLYAGTGDLL
                     LVRRPWSLKNRPVVVGVDGSDASYAALERAASIAAAHDTPLEAVAVYDPFFHSAVFPV
                     IAGALPEEAQRRFNFPAQEQLHDEIIDRGLEHLYRRNLERGAALAQALGARVHTAVVA
                     GRVCPQVHHYAAARGAGLLVLGRYGLHREEESLIGSNTLNLARMSTTNVLVVAPRETP
                     VTVPDLPRDAIPWTPEAEQVLSRVPPFARKMARMAVEEHVRARGLSRVTEDAVRDVAR
                     RSGRGGGQDAGP"
     gene            4392..4592
                     /locus_tag="PLV90_05590"
     CDS             4392..4592
                     /locus_tag="PLV90_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96623.1"
                     /translation="MQVPDARVVVFTPTKRFAPDFHRHILQGRIVGQTIRPGDRILVY
                     EVAETVPDGAVRVTRSTHLEFR"
     gene            4602..5540
                     /locus_tag="PLV90_05595"
     CDS             4602..5540
                     /locus_tag="PLV90_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF96624.1"
                     /translation="MAVALRDVTKRFGSVRALDGVTLDVGKGEILGLLGPNGSGKSTL
                     LKVIARLIPPDEGTVLLPANGRAMMRRTGMLFDHTAHWEALTGYENARFFARSYGMEP
                     GAAHDRLESLFSLLDLRDRWDDPVSTYSYGMRRKLGLIEALAHEPSLILLDEPSIGLD
                     YRARVTLAGLLREAARGGAAIVFSTNDVNEAADLAHRVALFDRGRMLVAGEPSVLVGS
                     LGATVTIDLRLKAPIDPRPLGQVSGIEGVGTEEREDGFHVTVIAAPDASGHSSLLARV
                     VHAVANEGGVVVGVDLHAPGLPDVFLAYTKEGADAP"
     gene            5530..6201
                     /locus_tag="PLV90_05600"
     CDS             5530..6201
                     /locus_tag="PLV90_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF96625.1"
                     /translation="MHPDSFFRKEILEDLRNRRGLLLKFVLPAGLLLPLMLADLPVSL
                     QAAGITVAIIFSGIFGSSIGLVRIRESGMLDRMAMLPISPRRLIAGYILANALLDLAQ
                     IAIPLAVFLILHPPAPEVVFCVAGAFASAVVAANALGVLAAVAPGSSSEGHLYAIITV
                     MAAIVLSGLVAPAPHTQGLLPFWYLHAALVTAPGAFALSPVLAGLILGSALAVSPRLF
                     GRTRG"
     gene            6494..7756
                     /locus_tag="PLV90_05605"
     CDS             6494..7756
                     /locus_tag="PLV90_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOF96626.1"
                     /translation="MVGLTLRRSSDLFGVSEDDIKKGLSETVFLRGLQNVLSGIARYG
                     ITMPQIVNAPFMVVWDFTHRCNLRCAHCYQDAQKALPHELDTDEAKRLVEELADAGVV
                     VIAFSGGEPLMRKDFFEVAAHAHKHDIYVALASNGTLITPEMAGRIKGAGVEYVEISV
                     DGKDAASHDAMRGIPGAFDRTIAGVRNSVAAGFYTCIATTVTRANYAEVPDIYALASD
                     LGVNRLMCFNFIPTGRGVDMAGQDISPEERRDLMRYLMARTREGKGPEALTTAPQIAA
                     VAVADEKGVPVGHFYAGEAIEGKTGLLADFIGGCGAGRLYCSIEPEGNVQPCVFMPIV
                     VGNVRDTPFLEIWHSAEVLQKLRDRSLLSGACADCGNKYICGGCRARGWAYFGDIHAP
                     DPGCINNRDYWESLLVGEVPVLPSQKKG"
     gene            complement(7753..11331)
                     /locus_tag="PLV90_05610"
     CDS             complement(7753..11331)
                     /locus_tag="PLV90_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOF96627.1"
                     /translation="MTKVTHLALLCAFVAIGLLISPVMAVEAASGEHIYRYLDHAQDH
                     PDVDGNMIVWEDDRDGDKDIYFGTIDGFRESPGYTGERITADPARQERPSISGNYIVW
                     QDNRHGNPDIYLYDRLNATETQLTTCTGRQWLPAISGNYVAWYDDSSGRTTIVLYDIA
                     GKTVKDVIDCDARTTIPGASIEFKPALSEKYVAWVEDEDARVRYYDIAAGTIAGTVST
                     SMAVQCWPSLSGSLLVWEDYRDGNAEIYMFDLDNPSGNEQQITSDASDQVAPAISGRI
                     IAWEDNRDKGPDETDGRGIYLYDLASGKELSVKLPDENYDEHFYPAVSGNTVVWQRGA
                     TPNSNLYIFVYEPDVPVDPALTDITVTPSSATLMVGETKGFVATALDQDGNAMPGIDI
                     AWSSSDPTVGTIDDDGVFTALVAGTATVTAASGDIAGTATVTVSDEEPVLTGLNVTPP
                     RATLAAGDSLKFIVTARDQNGNVMPAGAVAWTSSDPAAGTIDDDGVFTALAAGTATVT
                     ATSGEVAATAAVTVIAAEPEFGMVVLSPPEVTLETGDTLQFEAVAFNSYGETVPAAAL
                     TWASSDPAAGTIDDDGVFTALAAGTTTVTAASGDIAGTAVVTVSSDEPVLSRIAITPP
                     AITLNAGDEETFVATAYDQHGTAMPADAVAWASSDPAAGTIDDGGLFIALDAGTTTIT
                     ATADGITGAADVTVVRPAPGVVVSPPAITLDEGETWLFTATLVDPEDDALFSGVVAWS
                     SHDETVGTIDEDGFFTALSEGAVTITATIDGSGEIGTAVVTVRPASAFPARIAISPSD
                     FTIAAGQDLRLTATVYDQYGDEMPGVAVAFESSDPHIGTIDDSGLFAALKDDSVVLTA
                     TAGDISGSARVTVEPSLAVPATIAIEPAKTVIAPGEIREFVATVFDQRDDGMDWVRVA
                     WSCSDAAIGTIDRAGLFTAYICGTADVKARAGGVEGIATVSVTTAPIPDDPGNNDESA
                     GWSGSSGGGDSGPAFDAGMGESLMRGETFTFSGITTSSVDSVAITAANSIPRLMVTVK
                     GTTAPSAAVPPDGDVYEYIEITLYWANPDDIESAVIVFTIPVDWLREHGMTPEDVGLM
                     RYVGGVWQPLDTVFLGEEDGAYRFRATTPGFSTFAIAAVAPENTTVTPEATATPGGEA
                     NTTINATAEPMATATTVPVTTAPAAPLVYAPFLAPLAFLLWRRRNH"
BASE COUNT         2252 a         4008 c         3868 g         2207 t
ORIGIN      
        1 tgcgaccaca acggctggaa acgcgaccac gaccaacacg acagcgggag cggggaccac
       61 cccctcttcc tctttggccg tgacgcccga ccatggccag gtcagccaga tatgggatct
      121 cctggagccg cccgccggcg atcgaaccgt ctgggagctt ctccaggcga ccgagacggc
      181 catccccttt gaagagacac ctgtctacgg caacttcacg atcttcatcg atctcctcca
      241 gacgacgggg gtgaaccagg tcctccaggg caacgattcg tacaccgtct ttgccccgac
      301 cgatggggca ttcgtagagc ttcctcccga cgatatgaac gccctgacca agacgacggg
      361 caaccagtcg ggagcaataa accgcaccgc agtcgccctg aaccatatcg tgccgggatc
      421 gtatacagag agcgcccttc gtaacgagac cacgctcgtc accctcgcgg gaagcaacct
      481 caccgtgaat gcaaccggca acgagacccg ggttggcaac gccacgatca tcatccccga
      541 tctcgtcgca aagaacggag ccatccaggg tatcaaccgg gtgctgctcc cggccgacac
      601 gggcggggca gcaaacctct cctgaccgcg ctgaaagatc tttcagggcg gtacgaacgc
      661 cggcgcggaa tggggagtgg ggtcgtatga cctcgcgggc ggtctttggt cgcaccgcag
      721 ggatgacggc agatcacccg atggtgcggg ataccggcaa aaaaacgtgt tctctcaatc
      781 ctgaccctct atgccccgga taacgtcaga cgatccggca caaccattat atattctcgt
      841 gccatgtccg gacccgcatg acaaaagtta cccttgtcat agcatccctc ctcatcatcg
      901 cactcattgc gggcgtgggt gcacaacagg cgggattgag caatgcaagc agtaccaacg
      961 agagttccaa ccagacgacg cagagcacgc ccggcaacgt aacggacctc acgaacgccg
     1021 gcgactcgct tgccgcgacg tttcagaaca acgaatccct gacgaacctg gcacccatcc
     1081 cgagttttac ggccgatatc ggccttgagc tcatcggtga gaacttcacc gcgcccatga
     1141 tggtcacgtc gcccgacgac gggaccgggc ggctctttgt cgtggaccag atcggggtcg
     1201 caaagatcgt cgatgtaaac gggaccgaac tccccgaacc gttcctggac ctccgggata
     1261 acctcgtgaa actcgacccg acctacgatg aacgcggcct gctctccata gcgttccacc
     1321 ccaattacag gaacaacggc aaggtctacg ctttctacag cgcgccctta agaccggaag
     1381 cccccgaggg ctggagttgc acgaatcaca tatccgagtt ccaggtcgat cccgataatc
     1441 cgaacaccgt gaacatgtcg tccgagaaag tcctgatgta catcgacaaa ccttacgaga
     1501 accacaacgg cggcgtgctc gccttcggcc ccgccgacgg ctacctctac atatcggtcg
     1561 gtgacggcgg gaaggcaaac gatgtcggga acgggcatac ccccggtatc gggaacgccc
     1621 aggatctcac gaagatctac ggcaagatcc tccggatcga tgtggacagc accgccaccg
     1681 gggtcacgat agttcagcag aaccagaccg agaaccagac cgggggcgcg agcgccaacc
     1741 ggacggagaa cccgcccgaa ccgacgtgga cgacgtttgc cggcagcctc tacggcatac
     1801 cgcccgacaa cccgttcgcc gagaatcagc ccgagatcct cgactcctac gcttacaaga
     1861 cagttccaca ggagatatac gcctacggat tccggaaccc cgcctacatg gcttttgacg
     1921 ccagcgacgg caacgcgctc tacgtcgcca tggcaggcca gaacctcttt gagtccgtct
     1981 taattgtcct gaacggcggg aactacggct ggaacatccg cgaagggacg cactgcttcg
     2041 acccgaacgc ccccacggcc ccgggagagt cctgcaacag caccggcctt ctgggagaac
     2101 ccctgatcgg ccccatcttt gagggcggcc acgatctcgg catcgtgatc gtcggcggtg
     2161 atgtctatcg cggaaacact gtcgtcgggc tccagggcag gtacatattc ggctactgga
     2221 gcgacagccg gacggtagga aacggcaccc tccttgccgc cacgccgccg gcagggtggg
     2281 ccgagggcgc actgccgccg accgccgcag gtctgacgcc ggaagataac gccatgtggc
     2341 aggcccagat agtgaacgcc accgcacaaa acagcgatac cctgggcatg ttcctccgcg
     2401 ggtttggcga ggatgccgac ctggacatgt acgtgctgac aagcgacgtg ggaggccctg
     2461 atgcctctac cagcaccggc aaggtctgga agatcgtgcc gcccaccatc gtcaatccgg
     2521 ccctgggtgc gggggcgctc gaggtcacgc cgactgtgga ggcagagaac cagaccgaaa
     2581 accagaccgg caccacggct gtaggaggag gaatgggagc gggtgcaaca cccctctcct
     2641 ccctctcttc atcggacccc gccggaaaca tcatggaagt ccttgcattg accgagaccg
     2701 gcatcccctt cgaggagacc cccgcgttcg ggaacttcac cctgttcaca ggcctcctcc
     2761 ggtcgaccgg cgtcgccgcc acgcttgagg gcccgggacc atacacggtg ttcgccccga
     2821 ccgatgatgc attcgagcgg atggaccccg atatccgcga accgatcctg aacggttccc
     2881 gcgacagtaa ggacatcgtg ctctaccaca tcgtgaacgg ttcctccacg ctcgccgatc
     2941 tcaagaacgt atcgacgctc cggaccctgc agggcgaaaa cctcaccata aacgctaccg
     3001 gaacggtcgt cagggtggag aacgccacga tcgcgatccc cgatatcatg gccgacaacg
     3061 gtgtcatcca gggtatcgat accgtcctca tcccggccga cacctgaccg ggacttctgc
     3121 aaaatatcac ccaaagcatt tatgccgttt gtgccgctct tatccctgtc atccaggatg
     3181 acgggtgtgc atgatgtacc agcagatatt cgtaggggtg gacggctctc cctgctccga
     3241 catggcgggg gatgcagctc tcgcccttac tgccggcggc aggggcaggt gcaccggctg
     3301 ccacgtgtat gccgcccgga tgcaccggca gcgctttgag gatatggagc ccggccttcc
     3361 cgggcagtac caggaagaag aacggctcga tgacctgcgg gcgacgcacg acgacctgat
     3421 atccgggggc atggcgctca tatcggatgc ctacctcgcg ccgctggcgg agaaggccgc
     3481 cgcacagggt atccccttcg aaccggtcat agccgaaggg aggggatacg tcgaggttct
     3541 ccgcctcatc cgggagcgcc ggccggatct tgcggtgctc ggcgcgaccg ggcacgggca
     3601 gacgccggag gtgcccctcg ggggccttgc cgagcgggtt ctcctgtatg ccggcacggg
     3661 cgacctcctt ctggtccgga ggccctggag cctgaagaac cgcccggtcg tcgtgggcgt
     3721 ggacgggagc gacgcgagct acgcagccct cgaacgtgcg gcctctatcg ccgcggctca
     3781 tgatacgccg ctcgaggcgg tggccgtcta cgatccgttc tttcattccg cagtctttcc
     3841 cgtcatcgcc ggcgccctgc cggaggaggc gcagcgccgg ttcaacttcc cggcccagga
     3901 gcaactccac gacgagatca tcgaccgggg gcttgaacac ctctaccggc gaaacctgga
     3961 gcgcggggcg gccctcgcgc aagccctggg cgcccgggtc cacacggcgg tcgtcgccgg
     4021 cagggtctgc ccgcaggtgc accactacgc cgcggccagg ggggcggggc tcctcgtcct
     4081 cgggcgctac gggctgcacc gggaagagga gtcgctgata gggtcgaaca ccctgaacct
     4141 tgcccggatg agcaccacga acgtcctcgt cgtcgcgccg agagagaccc cggtcacggt
     4201 gccggacctg ccccgcgacg caataccctg gacgccggag gcggagcagg tcctctcgcg
     4261 ggtgccgccg tttgcgcgga agatggcacg gatggcggtc gaagagcatg tccgggcccg
     4321 gggcctctcc cgggtcacgg aggatgccgt ccgggacgtg gcccggcggt ccggcagggg
     4381 aggtggtcag gatgcaggtc cctgatgccc gggtggtggt ctttaccccg acaaagaggt
     4441 ttgccccgga ctttcaccgg catatcctgc agggacggat cgtggggcag accatccgcc
     4501 cgggcgaccg gatcctcgtc tatgaggtcg cagagaccgt gcccgatgga gcggtgcggg
     4561 ttacccggtc cacgcacctt gagttccggt gagggggagg gatggctgtc gccctgcggg
     4621 acgtgacgaa acggttcggg tccgtccggg cgctcgacgg tgtcaccctc gatgtgggga
     4681 agggggagat cctcggcctc ctcggcccca acgggtcggg gaagtccacc ctgctcaaag
     4741 tcatcgcccg gctcatcccc cccgacgagg ggactgttct gctcccggca aacggccggg
     4801 cgatgatgcg ccgaaccggt atgctctttg accataccgc ccactgggag gcgctcaccg
     4861 gctacgagaa cgcccggttc ttcgcccgct cctacggcat ggaaccaggc gcggcgcacg
     4921 accggctcga gtcgctcttc tcgcttctcg acctccggga tcgatgggat gacccggtct
     4981 ctacctactc gtatgggatg cggcgaaaac tcggccttat cgaggccctg gcgcacgaac
     5041 cctccctcat cctgctggat gagccctcga tcggcctgga ttaccgggcg cgggtcaccc
     5101 ttgccggtct cctccgcgag gcggcccggg ggggtgctgc gatcgtcttc tccacaaacg
     5161 acgtgaacga ggcggccgat cttgcccacc gggtcgccct ctttgaccgg ggcaggatgc
     5221 tcgtcgcggg agaaccgtcc gtgctcgtcg gatcgctcgg cgctacggtg acgatcgacc
     5281 tccggctgaa ggcgccgatc gatccccggc cgctcgggca ggtttccggc atcgagggcg
     5341 tgggcacgga ggagcgggag gacgggtttc acgtgacggt tatagccgcg ccggatgcat
     5401 ccgggcactc atccctcctc gcccgggtcg tccacgcggt ggcgaacgag ggcggggtgg
     5461 tcgtgggcgt cgatctccat gccccggggc tcccggatgt cttcctcgcg tacacgaagg
     5521 agggagcgga tgcaccctga ctcgttcttc agaaaggaga tccttgaaga cctccggaac
     5581 cgccgggggc tcctcctcaa gttcgtcctg ccggcggggc tcctgctccc cctcatgctc
     5641 gccgatctgc ccgtctccct gcaggccgcc ggcatcacgg tcgccatcat cttctccggc
     5701 atattcgggt cgtcgatcgg tctcgtccgc atccgcgaga gcggcatgct cgaccggatg
     5761 gccatgctgc ccatctcccc ccggcggctg atcgccggat acatactcgc aaacgctctc
     5821 ctcgatctgg cacagatagc catccctctc gccgtcttcc tgatcctgca tcccccggcc
     5881 ccggaagtcg ttttctgcgt ggccggagca ttcgcctccg ccgtggttgc ggcaaacgcc
     5941 ctcggcgtcc tggcggcggt cgccccgggc tcgtccagcg aggggcacct ctacgccatc
     6001 atcacggtca tggcagcgat cgtcctctcc ggactcgtcg cgcccgcccc gcatacgcag
     6061 gggctcctcc cgttctggta ccttcatgca gcactcgtca ccgcccccgg ggcgttcgcc
     6121 ctctctccgg tccttgccgg cctcatcctc ggctccgcgc tcgcggtctc gccgcggctg
     6181 ttcggcagaa ctcgagggtg agagcatcgg aggtttcggc gggcaacctt taagccatct
     6241 cagccctccg gggtgatccg gatcaccccc ggataatgcc atccccgggt gcaggagaga
     6301 tgagcaggca atgacagatg accgtattaa tgcgcttccg gcggtccggc ggtggctcgg
     6361 aaacccggtt gcccgtgccc tcctccggtt catcgcccgg gatgatgagt gtggaaatcg
     6421 cctcgataac gcgattcgat attaccttga ccagaacgac gggttatgct ggaggtgtcg
     6481 gctcgcgggc catatggtgg ggctcaccct ccgccggagc agcgacctct tcggggtgag
     6541 cgaggatgac atcaagaaag gtctctccga gaccgtattt ttacgggggc tccagaacgt
     6601 gctctccggt atcgcccgct acggcatcac catgccccag atcgtgaacg cccccttcat
     6661 ggtggtctgg gacttcaccc acaggtgcaa ccttcgctgc gcccactgct accaggacgc
     6721 gcagaaagcc ctcccccacg aactcgatac cgatgaggcg aaacgtctcg tcgaggaact
     6781 tgcggatgcc ggcgtggtgg tcatcgcctt ttcgggcgga gagcctctca tgcgaaagga
     6841 cttcttcgaa gtggccgccc atgcccacaa acacgacata tacgtggcgc tcgcctcaaa
     6901 cggcaccctc atcacaccgg agatggcggg gcggatcaag ggtgccggcg tcgagtacgt
     6961 ggagatatcc gtggatggga aggatgcggc gagccacgat gccatgcggg gcatcccggg
     7021 cgcgttcgac cggaccatcg caggcgtcag gaactccgtc gcggcggggt tctacacctg
     7081 catcgcaacc acggtcaccc gggcgaacta cgccgaggtc ccggatatct acgcccttgc
     7141 atcggacctc ggtgtgaacc ggctcatgtg cttcaacttc ataccgacgg ggaggggcgt
     7201 ggatatggcc ggccaggata tatccccgga agagcgcaga gatcttatgc gctacctgat
     7261 ggcccggaca cgggagggaa agggtcctga ggcgctcacc accgccccgc agatcgcggc
     7321 cgtggcggtt gccgatgaga aaggggttcc cgtcgggcac ttttacgccg gggaggcgat
     7381 cgaggggaag accgggcttc ttgccgactt catcggcggg tgcggcgcag ggaggctcta
     7441 ctgcagcatc gagcccgaag gaaacgtcca accctgcgtc ttcatgccga tcgtcgtcgg
     7501 aaacgtccgg gatacgccgt tccttgaaat atggcactca gcggaggtgc tgcagaagtt
     7561 gcgggacagg agcctgctct ccggggcatg cgccgattgc gggaacaagt acatatgcgg
     7621 cgggtgcagg gcccgcgggt gggcatactt cggggatatc cacgcgccgg accccgggtg
     7681 catcaataac cgcgactact gggagtcgct tctcgtgggc gaggtgcccg ttctcccgtc
     7741 ccaaaaaaag ggttagtggt tccttctccg ccagaggagg aaggcgagcg gggcaaggaa
     7801 gggtgcgtag acgagcggtg ccgcgggcgc cgtcgtcacc ggcacggtgg tcgccgttgc
     7861 catcggttcc gcagtcgcgt ttatcgtggt gttcgcctcc cctcccggcg tggcggtcgc
     7921 ctccggcgtt acggtcgtgt tctccggcgc cacggcagcg atggcaaacg tggagaaccc
     7981 gggggtggtc gcgcggaagc ggtacgcccc gtcttcctca ccgaggaaga cggtatccag
     8041 gggctgccag acgccgccga catagcgcat gagcccgaca tcctccggtg tcatgccgtg
     8101 ctcgcggagc cagtcgacgg ggatggtgaa gacgatcacc gcgctctcga tgtcgtcagg
     8161 gtttgcccag tagagggtga tctcgatgta ctcgtagacg tcaccatcgg ggggcaccgc
     8221 cgcagagggc gctgtggtcc ctttcacggt caccatcaac ctcgggatgc tgtttgccgc
     8281 cgtgatggcg acgctgtcga ccgatgatgt ggtgatgccc gagaacgtga acgtctcgcc
     8341 gcgcatcagg ctctcgccca tgcctgcgtc aaaggcggga ccgctgtccc cgccgccgct
     8401 gctaccgctc caaccggccg attcgtcgtt gtttccggga tcgtcaggga tcggtgcggt
     8461 cgtgacgctg accgtcgcga ttccctccac gccgccggcc cgggccttca cgtccgccgt
     8521 cccgcatatg tatgctgtaa agaggccggc cctgtcgatg gtgccgatgg ctgcatcaga
     8581 gcacgaccag gcgaccctga cccagtccat cccgtcgtca cgctggtcaa agaccgtggc
     8641 gacgaactcc cggatctccc cgggcgcaat cacggtcttc gcaggctcga ttgcgatggt
     8701 ggccgggact gcaagggagg gttcaacggt cacgcgggcg gagccggata tatcgcctgc
     8761 cgttgcagtg aggaccacac tgtcgtcctt gagggcggca aagaggccgg agtcatcgat
     8821 ggtgccgata tgcgggtcgc tgctctcaaa ggccaccgct acgcccggca tctcgtcgcc
     8881 gtactgatca tacacggtcg cggtgagcct caggtcctgc ccggcggcga tagtgaagtc
     8941 agacgggctg atcgcgattc gcgccgggaa cgccgatgcc ggccggaccg tcacgaccgc
     9001 ggtcccgatc tctccggatc cgtcgatcgt cgcggtgatg gttacggcgc cctcactgag
     9061 cgcggtaaag aacccgtctt cgtcgatcgt gccgacggtc tcgtcgtgcg acgaccaggc
     9121 gaccactccg gagaatagag cgtcgtcctc cggatcgacg agagtcgcgg tgaagagcca
     9181 ggtctctccc tcgtccaggg tgatcgccgg cggggagacg acgacgcccg gggccggccg
     9241 gacgaccgtt acgtccgccg ctccggtgat gccgtcggcc gttgcggtga tggtcgtcgt
     9301 gcccgcatca agggcgatga agagcccgcc gtcatcgatg gtgccggcgg ccggatcgct
     9361 gctcgcccag gcgacagcat ccgcaggcat ggcggtgccg tgctggtcgt aggcggttgc
     9421 gacgaacgtc tcctcatcac cggcgttcag ggtgattgcc gggggcgtga tggcgatccg
     9481 tgagaggacc ggctcatcgg aggataccgt gacaaccgcg gtgccggcta tatcgcctga
     9541 tgccgcagtg acggtcgtcg tacccgcggc aagggcggta aagacgccgt catcatcgat
     9601 cgtcccggcg gccggatcgc tgctcgccca ggtgagggcg gcggccggca cggtctcgcc
     9661 gtaggagttg aacgcgacgg cctcaaactg cagggtgtcg ccggtctcga gagtgacctc
     9721 cggcggcgag agaacaacca taccaaactc cggttctgca gcgatgacgg tgacggccgc
     9781 cgttgcggcg acctcgcccg atgtcgcagt gacggtcgcc gtacccgcgg caagggcggt
     9841 aaagacgccg tcatcatcga tcgtcccggc ggccggatcg ctgctcgtcc aggcgacagc
     9901 acccgcaggc atgacgttgc cgttctggtc gcgggcggtc acgatgaact tcaggctatc
     9961 gccggctgcg agggtggccc ggggaggcgt gacgttcagg cccgtgagca ccggttcttc
    10021 gtcgctgacg gtgacggtcg cggtgccggc tatatcgcct gatgccgcag tgacggtcgc
    10081 cgtacctgca acgagggcgg taaagacgcc gtcatcatcg atcgtcccga cggttgggtc
    10141 gctgctcgac caggcgatat cgatgccggg catggcgttg ccgtcctggt caagagcggt
    10201 tgccacgaac cctttcgtct cgccgaccat gagcgtggcc gaggatggcg tgaccgtgat
    10261 gtccgtcagg gccgggtcga ccggcacgtc cggttcgtag acgaagatgt agaggtttga
    10321 gttaggggtc gcgccccgct gccagacaac agtgttgcca ctgacggcag ggtagaagtg
    10381 ctcgtcgtag ttctcatccg gtagctttac cgacagctct ttcccggatg caagatcgta
    10441 caggtagatg ccgcgtccat ccgtttcatc cggcccctta tcccgattgt cctcccaggc
    10501 gatgatcctg ccgctgatcg ccggcgcgac ctgatcggag gcgtcggagg tgatctgctg
    10561 ctcattgccg gacgggttat cgagatcgaa catgtagatc tcggcgttgc cgtcccggta
    10621 atcttcccag accaggaggc tcccggagag cgagggccag cactggaccg ccatgctcgt
    10681 ggagaccgtg cctgcgattg taccggccgc aatgtcgtag taccggaccc tcgcatcttc
    10741 gtcttccacc caggcgacat acttctccga gagtgcgggc ttgaactcga tcgatgcgcc
    10801 ggggatcgtc gtccgtgcat cgcaatcgat gacgtccttc accgtcttgc cggcgatatc
    10861 gtagaggacg atggttgtcc tgccgctgct atcgtcgtac caggcaacgt agttcccgga
    10921 gattgcaggc aaccactgcc tgcctgtgca ggtggtgagc tgcgtctcgg tcgcgttgag
    10981 gcgatcgtag aggtagatat cagggtttcc gtgccggtta tcctgccaga cgatgtagtt
    11041 gcccgatatg gacggcctct cctgcctcgc aggatcggcc gttatcctct cgccggtata
    11101 gccgggcgat tcccggaacc cgtctatcgt accgaagtag atgtccttgt ctccgtccct
    11161 atcatcttcc cagacgatca tgttgccgtc gacatcgggg tgatcctgcg catggtcgag
    11221 gtagcgatag atatgctccc ccgacgcagc ctctacagcc attaccggcg atatgagcag
    11281 cccgatggcg acaaacgcgc acaataatgc caggtgtgta acctttgtca tattcctctc
    11341 ccttgtctct tctccacccc cgcccacaaa gaccacggtg cataccctcc ggtatgcacg
    11401 aagggctcgc taaagcaggt gctccattgg gcacccggca gacggagtgc gaaatcctat
    11461 ttaaagtccg gtcataaggt agccggtgaa agggtgaata gcagcataaa tatatatctt
    11521 ttacggtgat ttattccaaa attattatat tataaactga aatttcctgt gcgctcccgg
    11581 ggggagagga gtcgggggac gggtcccctc agccggccgg ggaggatggt tcagatcgag
    11641 gagcaccgtg aaccgttgcc tccggcaaag cccccaaacg acaactttcc ccatgaatcc
    11701 cctgcgatcg gccggaaggt gtatcgcccg gcacgcaccc ggggaggcgg gagtacacgg
    11761 gctgaggtca ctccctgcac ccataccgga gcgctaccca tatcagcaca acatatcggc
    11821 agaagtatag cagggataat ccgggcttat cctgatccca aaagtccctc ccataagggt
    11881 gaacggttta ccgtatcacc gggtaccccg gggagggggc gaaaccagac aagggcatgc
    11941 ctttacttta ctattggcct ttaattctgg ggccgctttt atcgtatcga atcatcgaaa
    12001 aatgatacgg tagcgcatct accgtaacgc ttttcgctgt tacggtagtt aacggtagtc
    12061 gttgacctca tccgcctcct cacccttatc ccggaggtgg aatcagggcg ccctcaatgg
    12121 ccacccatcg cgagattgca gcgttgacga atagagaagg gctatcttat cacggggtcg
    12181 gagatagcgg caaccatcgt cggggccggc gtgaaagcgc ccccctgccg gcgacgtttg
    12241 caacccggat catccctccg ggatccgggt cctcccctca tccggtatag ccccctatgg
    12301 atgccggaga accgcctcac gcgaaccgcc ggatg
//
LOCUS       DAOSII010000056        12237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_5218_length_12237_cov_56.111312, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000056 DAOSII010000000
VERSION     DAOSII010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 12237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12237
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5218_length_12237_cov_56.111312"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PLV90_05615"
     CDS             complement(<1..488)
                     /locus_tag="PLV90_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOF96628.1"
                     /translation="MLPGEVYPPAEDSSLLLRAALREVRGADRVLEVGTGSGYVAAGL
                     SGRAAAVVVTDINPHAAACARARGLAAVRTDLFAGLSGPFDLILFNPPYLPTAPEERI
                     DDWLEYALDGGPTGRVVIERFIADAGRVLAPFGRVLLLVSSLTGLDEVRDLFARAGFV
                     SFV"
     gene            complement(491..1264)
                     /gene="rsmA"
                     /locus_tag="PLV90_05620"
     CDS             complement(491..1264)
                     /gene="rsmA"
                     /locus_tag="PLV90_05620"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOF96629.1"
                     /translation="MSAPRDQHFLIDRRAVERIVGFADVSGRRVLEIGPGEGILTRAL
                     LDRGANVVAVEIDPALVEELDLAFADAIDEGRLRIVSGDARKVDIPPFDIVVANLPYS
                     ASSKITFRLLDIGFETAILMYQKEFAQRMIARPGTPDVGRLSVMVQTYASVKPLLELS
                     PSAFRPQPQVRSWVVRVTPHEPPYPIADKRVYADVVRVLFSHRRKTVRKGLRSGKEFF
                     PLDVIERAIAALPDDLLQRRPENLTLCEFALVANTLAGC"
     gene            complement(1261..1824)
                     /locus_tag="PLV90_05625"
     CDS             complement(1261..1824)
                     /locus_tag="PLV90_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF655 domain-containing protein"
                     /protein_id="HOF96630.1"
                     /translation="MKAERKEENAVVIDVLPMGYMSDQRPVYKREPVVLAVGVDQFKL
                     LELVPKAGADIQIHDRVYIGDAERDKIERVKRRIGYEDLTATAKLELPFVVEQIVHEN
                     EQRFVDFFNKSLPITPKFHMLHLLPGIGKKLMWEVIEQREKKPFESFADISGRIKSLP
                     HPARMIVSRVLREIEDPNEKYHVFTLK"
     gene            complement(1842..2192)
                     /locus_tag="PLV90_05630"
     CDS             complement(1842..2192)
                     /locus_tag="PLV90_05630"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase Rpb4 family protein"
                     /protein_id="HOF96631.1"
                     /translation="MKVKRIVSEERMLLPELRDTLLAVEATRLGSGEEMSYELRKSIE
                     HANHLSKTSSDKARDLVGELMQFEKMKEEIAYRIANLMPRTRDELRAIYAKERFNLTA
                     EELDRILELVVTHF"
     gene            complement(2214..2507)
                     /locus_tag="PLV90_05635"
     CDS             complement(2214..2507)
                     /locus_tag="PLV90_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21e"
                     /protein_id="HOF96632.1"
                     /translation="MAHHNGPRKKTRYKFKKDLRRRGIPPVTSVIQDFEVGQKVHVVV
                     EPSVQKGMPHRRFHGKTGTVIGQRGRAWVLEVRDGDTMKQVIARPQHLKAQKV"
     gene            complement(2511..3749)
                     /locus_tag="PLV90_05640"
     CDS             complement(2511..3749)
                     /locus_tag="PLV90_05640"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(54/55) synthase Pus10"
                     /protein_id="HOF96633.1"
                     /translation="MDIIETVGAILEYGETCNHCLGRFFGKRSFSLTNEERGRALRIT
                     REIAVNEPHSEPESESCWICGGELSRTDAWAAKVVEAVQGIEFATFLIGTRVPPLIAE
                     SEEMVWSDLALRDPEPLKAEMNREVGKAVSALIGKTADLKRPDIVVILDIAEGTVEVQ
                     VNPVFFVGRYLKYERGIPQTHWDCRACKGAGCEKCDFTGKQYADSVEELIGRPVIEAF
                     DAENAVLHGAGREDIDARMLGSGRPFVMEVEAPRRRSVDLAALEEDINEKAGGRVAVH
                     LAGWADRKTVQSLKSDKAHKKYRILVEIDGPVTQDEFRAALDQLKGVTIRQRTPLRVA
                     HRRADKVRERDVLDIRCTGAQDDRYWVEVVGEAGLYIKELISGDNDRTQPSLARILGR
                     TARVVSLDVVLVETANEFGE"
     gene            complement(3737..4318)
                     /gene="trmY"
                     /locus_tag="PLV90_05645"
     CDS             complement(3737..4318)
                     /gene="trmY"
                     /locus_tag="PLV90_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (pseudouridine(54)-N(1))-methyltransferase
                     TrmY"
                     /protein_id="HOF96634.1"
                     /translation="MKRFAVVGHRAATSGTFSLNDLAGSAGRMDVLCRCINASFFLSH
                     DLRRDVECYLILCGEPGPEKTVLFSGSALRYLSPDERSSAALVKKALALPCGDEFRES
                     TPGVYVRRGGFTRLLAEVPFAVLDEAGEDIRAAPALPEAYLLSDHLNFTPEEEDLIAA
                     YPRYSVGPRSLHADHTITVLLNEMDRRESGWIS"
     gene            complement(4315..>5463)
                     /locus_tag="PLV90_05650"
     CDS             complement(4315..>5463)
                     /locus_tag="PLV90_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein Srp54"
                     /protein_id="HOF96635.1"
                     /translation="EEPPKGMGVREHVLRIVYQELVRLMGAPGAVALGPQTILMAGLQ
                     GSGKTTTTAKLARYFQRKGLRVGVICADTFRPGAYDQLKTLCDRIGIPCYGDPGEKDA
                     LRIVREGLPKLRKQYEVIIIDTQGRHALEENLIEEIISINDIARADHRWLVIDAALGQ
                     QASEQARRFHEAIGIDGVLVTKMDGTARGGGALSAVAETQSGIVFIGSGETIEDLERF
                     DPDGFISRMLGMGDLKALVERAEEAVSAEDIDVNAMLKGKFTLRDMYKQLEALNRMGP
                     LKQIMSMLPLGGMQIPDDAYDITSSKMQRYKVIMDSMTAPELDDPSVIGSSRIHRIAR
                     GAGVAPEDVRDLIKYYKIMQRALKGMRGMGGGKFNMQRMMKKFGGPSR"
     assembly_gap    5464..5563
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5564..6546)
                     /locus_tag="PLV90_05655"
     CDS             complement(<5564..6546)
                     /locus_tag="PLV90_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOF96636.1"
                     /translation="MFDSLKKKLQNIRTKFASNIEEAATVQPAVEQPAPPPTVEPPLP
                     PVITPAPPLPGKPLAPPAAREEKREDPTFFNRLKILVREREILLQEKDIEEPLMELEM
                     VLLENDVALPVTDEIIARMKKDLVGRHRKIGASVDDLVVATLRSALCGVLGDGLDLRD
                     YIREHERPVKILFTGVNGTGKTTTVAKVGHYLQKNGFTVVIGAGDTFRAGAIEQIRTH
                     ADRLDIKVIQHQAGADPSAVLFDAVQYAKAHEIDVVLADTAGRFHNRANLMNQLEKIR
                     RVMKPDLVVYVDEAVAGNDAVIRAGEFNRAVGTDAVVLTKADMDSKGGAAIS"
     gene            complement(6546..6995)
                     /gene="pfdA"
                     /locus_tag="PLV90_05660"
     CDS             complement(6546..6995)
                     /gene="pfdA"
                     /locus_tag="PLV90_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit alpha"
                     /protein_id="HOF96637.1"
                     /translation="MNQADPREIQTLQMYLNEYGQQIEILTQQLGMIEQQRIEAVAAI
                     ETLRALQESTDGVVLLPIGGGALLRVQVLDAGHVIVNIGADVSVERASADAIEYLGDR
                     ITELEAVGKKVAGSVEQLQGQATEISRRIEAAYRGIRQGEAGQGGIR"
     gene            complement(6997..7188)
                     /gene="rpl18a"
                     /locus_tag="PLV90_05665"
     CDS             complement(6997..7188)
                     /gene="rpl18a"
                     /locus_tag="PLV90_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18Ae"
                     /protein_id="HOF96638.1"
                     /translation="MENQTFEVVGACRINNAWKPYRKVVEAPNENQAREWVFADIGSK
                     HRLKRSYITIESVNVVAGE"
     gene            complement(7196..7858)
                     /locus_tag="PLV90_05670"
     CDS             complement(7196..7858)
                     /locus_tag="PLV90_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-6"
                     /protein_id="HOF96639.1"
                     /translation="MTGTIDLLGDPNIGVYARVFEDIAIVDPRVPGEFTEALGRELSV
                     DIVATTVQESGIIGSLVAGNSRGLVVSGLAAADELAVLAEHREVLLLEGSMNAAGNVI
                     LANDYVAAVHPDMDVGVAEEIGSFLGVPVIRLTLGGIKTVGMAGCATNKGILAHPRTN
                     ETEIATLERVVDLPIGLGSVNMGSGLVGTGLLANSKGYIAGSFTTGFELGRIEEVFGF
                     LE"
     gene            complement(7860..8123)
                     /locus_tag="PLV90_05675"
     CDS             complement(7860..8123)
                     /locus_tag="PLV90_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31e"
                     /protein_id="HOF96640.1"
                     /translation="MAEALKEHIYIIPLREVKRSPRWKRGNTAIKDIRAFLERHMKSA
                     DVKLDRSINEKVWENGSGKPPRKIRVRAMKFEDGQVQAELAEE"
     gene            complement(8128..8280)
                     /locus_tag="PLV90_05680"
     CDS             complement(8128..8280)
                     /locus_tag="PLV90_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L39e"
                     /protein_id="HOF96641.1"
                     /translation="MSKLTKGRKIRLGKACEQNRRVPAWVMIRTNRAVSSHPKRRNWR
                     RSSLKV"
     gene            complement(8277..8897)
                     /locus_tag="PLV90_05685"
     CDS             complement(8277..8897)
                     /locus_tag="PLV90_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha hydrolase"
                     /protein_id="HOF96642.1"
                     /translation="MEAGLLFSGGKDSALAAVMLARDYGVELNTCVFDPARDVPAVRA
                     AAAALHLPFRRRVLGRDLLEEAADMLLVCGYPNDAITMVHRAAIEALAGEYRVVGDGT
                     RREDRVPRLERSTVQHLEMTTGSSYVRPLLGYGKPEVERLAGRLLVVRYGETGSIGNG
                     DYEQEIREAIRARGIDPAPFFPAGHLQSLVVGMRTTGNGDTGVRQL"
     gene            complement(8897..9235)
                     /locus_tag="PLV90_05690"
     CDS             complement(8897..9235)
                     /locus_tag="PLV90_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOF96643.1"
                     /translation="MVDDELAELRRRKMEQMQRQAMDQQAMEDEVERQRQIESQIRVV
                     LMEILEPEARERLNTIKLTRPEFAKAVEQQLVVLAQSGRVRQRITDDQLKALLTQLTP
                     SKKEFRITRK"
     gene            complement(9253..9702)
                     /locus_tag="PLV90_05695"
     CDS             complement(9253..9702)
                     /locus_tag="PLV90_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19e"
                     /protein_id="HOF96644.1"
                     /translation="MTTVYDIPADMLIRQAAEELKKNPAIQPPDWADFVKTGVHKEMP
                     PENDDWWYVRAAAVFRRIYTDGPVGTQRMRSIYGGKHDRGSAPSRFKRGSGSIVRKIF
                     QQLEAAGYVAHTSEGRTVTAAGRSFLDNVANNVKPQVSGISPGIAKY"
     gene            complement(9754..10014)
                     /locus_tag="PLV90_05700"
     CDS             complement(9754..10014)
                     /locus_tag="PLV90_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HOF96645.1"
                     /translation="MSRESFQDIKPTVWVGKRGISSLMIDEIRRQLEDRKIVKVRWLR
                     NTEVDPEEVAASAGAVLIEVRGRTFVLAERRSRSPGRNSRNI"
     gene            complement(10011..10328)
                     /locus_tag="PLV90_05705"
     CDS             complement(10011..10328)
                     /locus_tag="PLV90_05705"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component 4"
                     /protein_id="HOF96646.1"
                     /translation="MADTTRRSGSRRLARERIAILFARAAEFYPDNPAWSNRCVELAR
                     KIGMRHRVRIDRPLKRRFCRRCSVYLVPGANARVRIYRGRVIVTCLACGHRSRYPVGR
                     RTS"
     gene            complement(10397..11008)
                     /gene="purN"
                     /locus_tag="PLV90_05710"
     CDS             complement(10397..11008)
                     /gene="purN"
                     /locus_tag="PLV90_05710"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOF96647.1"
                     /translation="MERKKRIATLASGRGSNFQAVIDAVAAGDIPAICVGLITDNPQA
                     YAVERARAAGIPVTVVDYARFPSKAAYEEALLAAMRGCRADLFVLAGYMRILGAAIVH
                     EFSGRMMNIHPALLPAFAGLHAQRQAIEYGVKVSGCTVHLVDEGMDTGPIVVQRCVPV
                     LSDDDESALADRILAEEHRALPLAVKLFCEDRLEVAGRKVRVR"
     gene            complement(11022..>12237)
                     /locus_tag="PLV90_05715"
     CDS             complement(11022..>12237)
                     /locus_tag="PLV90_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOF96648.1"
                     /translation="FFSSSEVALISITRAKVRALLNQGRKGAEALDVLKRSTDTILIT
                     ILIGNNIANVAAASLATAIAIGIYGDVGIGIATGVTVILMLVFGEIGPKMYASRHTES
                     LALSVARPILYLSKALYPVLWISDRINRQFAFRPGVTEPVVTEEEIKEWIDVGEEEGT
                     IEEQERDMLYSVLRFGDTTVREVMTPRIDVVMIEDANTIEGALALFNETGFSRIPVYH
                     EQIDNVVGLLNVKDVFAAVVRQQTSAMIRDVMYEPYFVPESKKIDELLKELQVKKQHM
                     AVVLDEYGSFAGIVTVEDMLEELVGEILDEFDEEEPEVQRVEENVYLVDARAWVDHLN
                     EDLHLALPLTDAYESIGGLVIDRLGHIPRRGEVVKLEESNITLVVMQMRGRRLVKVKL
                     IVAPPPAPEESR"
BASE COUNT         2062 a         4006 c         3614 g         2455 t
ORIGIN      
        1 acgaacgaga cgaaccccgc ccgggcaaag aggtcccgga cctcatcgag cccggtcagg
       61 gacgagacaa ggaggagaac ccgcccgaag ggcgcgagca cccggccggc atcggcgatg
      121 aaccgctcga tcaccaccct ccccgtcggc ccgccgtcga gggcgtactc cagccagtcg
      181 tcgatccgct cctcgggagc cgtcgggagg tagggcgggt tgaaaaggat gaggtcgaac
      241 ggccccgaga gccccgcaaa gaggtcggtc ctgaccgccg cgagaccgcg ggcgcgggcg
      301 cacgccgccg cgtgcgggtt gatatcggtc acgaccacag cggccgcccg acccgagagg
      361 ccggccgcga catacccgct cccggtcccg acctcaagca cccggtcagc ccccctgacc
      421 tcccggagcg ccgcccggag gaggagagac gaatcttcgg cgggaggata gacctcgccc
      481 gggagcatcc tcagcacccg gcaagcgtgt ttgcaaccag ggcaaactcg cacagtgtca
      541 ggttctcggg ccggcgctgc aggagatcgt cgggcagcgc cgcgatcgcc cgctcgatca
      601 cgtcgagcgg gaagaattct ttcccgctcc ggaggccttt ccggacggtc ttacgccggt
      661 gggagaagag caccctgacg acgtcggcat agacccgctt atccgctatc gggtagggcg
      721 gctcgtgcgg cgtcacccgg acaacccagg agcggacctg gggctgcggc cgaaacgcgg
      781 aaggcgagag ctcaaggagc ggtttgacgg acgcataggt ctggaccatc accgagagcc
      841 gcccgacatc cggtgtcccc ggccgcgcga tcattcgctg ggcaaactcc ttctggtaca
      901 tcagaatggc cgtctcaaac ccgatatcca gcaggcggaa cgttatcttc gaagaggccg
      961 agtacgggag gtttgcaacg acgatatcaa acggcgggat gtccaccttc ctcgcgtcgc
     1021 cggaaacgat ccggaggcgc ccctcatcga tagcatcggc aaaagcgaga tcgagttcct
     1081 ccacgagagc cggatcgatc tcgaccgcga cgacattcgc accgcggtcg aggagagccc
     1141 gggtgaggat tccttcgccg ggcccgatct cgagcactcg ccggccggat acatcggcaa
     1201 acccgacgat cctctcgacg gccctccgat cgatgaggaa gtgctgatcc cgtggagcac
     1261 tcatttcagc gtgaagacat ggtacttctc gttcggatcc tcgatctcgc gcaggacccg
     1321 gctgacgatc atccgggcgg ggtgcgggag cgatttgatc cgccccgata tatccgcgaa
     1381 gctctcgaac ggcttcttct cccgctgctc gatcacctcc cacatcagtt tcttcccgat
     1441 gccggggagc aggtggagca tatggaactt cggcgtgatg ggaagagact tattgaagaa
     1501 atcgacgaac cgctgctcgt tctcgtggac gatctgctcg acaacaaacg gcagctccag
     1561 tttcgccgtc gccgtcaggt cttcgtagcc gatccgccgc ttgacacgct cgatcttatc
     1621 ccgctccgcg tcaccgatgt agacgcggtc gtggatctgg atatccgcgc ccgctttcgg
     1681 gacgagctcg agcagtttga actggtccac gccgacggca aggacaaccg gttcgcgctt
     1741 gtagacggga cgctggtcgc tcatgtatcc catcgggagg acatcaatga ctaccgcgtt
     1801 ctcttctttc ctctcagcct tcatcgacat caccccgttg gttagaagtg cgtcactacc
     1861 agctcgagga tcctgtcaag ctcttccgcg gtgaggttga accgctcttt ggcatagata
     1921 gcccgcagtt cgtcccgggt ccgcggcatc aggttcgcga tgcggtaggc aatctcctct
     1981 ttcatcttct caaactgcat gagctcgccg acgagatcgc gggctttatc ggatgatgtc
     2041 ttcgagagat gattggcatg ctcgatactt ttgcgcagct cgtaggacat ctcttctccg
     2101 gacccaagcc gggttgcctc cacggcaagg agtgtatcac gcagttccgg aagcagcatc
     2161 cgctcttcgc tgacgattcg ttttactttc atgccaatca ctactgttga gggttatacc
     2221 ttctgcgctt ttagatgttg cggtctcgca atcacctgtt tcatcgtatc gccgtccctg
     2281 acctcgagca cccatgcgcg cccgcgctgt ccgatgaccg tgccggtctt tccgtggaat
     2341 ctccggtggg gcatgccctt ctggacactc ggctcgacca cgacatgcac cttctgcccg
     2401 acctcgaagt cctgaatcac cgaggttacg ggggggatac cgcgtcttct gaggtccttc
     2461 ttgaacttat accgcgtctt cttgcgtggt ccattgtgat gtgccatgat ttactcacca
     2521 aactcgtttg ctgtttcgac cagcaccaca tcgaggctga caacacgtgc ggtgcgcccc
     2581 aggatccggg caaggctggg ctgggttctg tcgttgtcgc ccgatatcag ctctttgatg
     2641 tagaggcccg cttcgccgac gacttcgacc cagtatctgt cgtcctgtgc gcctgtacac
     2701 cggatatcga ggacatcgcg ttcccgaact ttatctgccc gtcggtgtgc cacccgcagg
     2761 ggggtgcgct gtcgtatcgt tacacctttt agctgatcga gggccgccct gaactcgtcc
     2821 tgagtcacag gaccgtcgat ctcgaccagg atcctgtatt ttttatgcgc tttgtcggac
     2881 ttaaggcttt gcaccgtctt ccgatccgcc catccggcca ggtggaccgc cactctcccc
     2941 ccggccttct cgttgatatc ctcttcaagg gccgcaaggt ccaccgaccg ccttcgcggc
     3001 gcctcgacct ccatgacgaa ggggcggccc gacccgagca tccgggcatc gatgtcctcc
     3061 cggccggcac cgtggaggac ggcgttctcc gcatcgaagg cttctatcac cggccggccg
     3121 atcagctcct cgacggagtc cgcgtactgt ttgccggtga agtcgcactt ctcgcacccg
     3181 gcgcccttgc aggcccggca gtcccagtgg gtctgcggga tcccccgctc gtacttcagg
     3241 tagcgcccga caaagaagac gggattgacc tgcacctcga cagtcccttc cgcgatatcg
     3301 aggatcacga cgatatcggg ccgtttcagg tcggccgtct tcccgatgag agctgatacg
     3361 gctttgccga cctcccggtt catctccgcc ttcagcggtt cggggtcgcg aagggcgaga
     3421 tcgctccaga ccatctcctc gctctccgct atgagcggcg gcactctcgt cccgataaga
     3481 aacgtcgcaa actctatccc ctgcaccgct tccacgacct ttgcggccca ggcatcggtg
     3541 cgggagagtt cgccgccgca gatccagcag gactccgact ccggctcgct atgcggctcg
     3601 tttaccgcga tctcgcgcgt gatccggagc gcccttcccc gctcctcgtt cgtcagcgaa
     3661 aacgaccgtt tgccaaagaa ccgcccgagg cagtggttgc aggtctcgcc gtactcaagg
     3721 attgctccta ccgtttctat gatatccatc cggactccct ccggtccatc tcgtttaaga
     3781 ggacggtgat ggtatgatcg gcatgcagcg accgcggccc caccgagtag cggggatagg
     3841 ccgcgatcag gtcttcctcc tcaggggtga agttcaggtg atcggagagg aggtatgcct
     3901 ccggaagcgc cggcgccgcc cggatatcct cccccgcctc atcgaggacg gcaaacggca
     3961 cctccgcgag gagtcgcgtg aacccgccgc ggcggacgta aacccccggt gtcgactcgc
     4021 ggaactcatc cccgcagggg agggcaagcg ccttcttgac cagggcggcg ctgctccgct
     4081 cgtccggcga gaggtagcga agagcgctcc ccgagaagag gacggtcttc tcgggcccgg
     4141 gctccccgca gaggatgagg taacactcga cgtcgcgccg cagatcgtgg gagagaaaga
     4201 acgaggcgtt gatgcaccgg cagaggacat ccatcctccc ggcgctcccg gcgagatcgt
     4261 tgaggctgaa cgtgccgctc gttgcggcac gatggcccac gacggcaaac cgcttcatcg
     4321 gctgggtccg ccgaacttct tcatcatgcg ctgcatgttg aacttgccgc cgcccatgcc
     4381 ccgcatgcct ttcagggcgc gctgcatgat cttgtagtat ttgatgaggt cgcggacatc
     4441 ctcgggcgcc acgccggcgc cgcgggcgat ccggtggatc cgcgagctcc cgatcaccga
     4501 ggggtcatcg agctcagggg ccgtcatcga gtccatgatc accttgtagc gctgcatctt
     4561 gctgctggtg atgtcgtagg cgtcgtcggg tatctgcatg ccgccgagcg ggagcatgct
     4621 catgatctgc ttcaagggcc ccatccggtt cagcgcctca agctgcttgt acatgtcccg
     4681 gagggtgaac ttgcccttca acatcgcgtt gacgtcgata tcctcggccg agaccgcctc
     4741 ttcggcccgc tcgacgaggg cttttaagtc gcccatgccg agcatccggg atatgaaccc
     4801 gtccgggtcg aaccgttcga gatcctcgat cgtctcgccg ctcccgatga agacgatccc
     4861 gctctgcgtc tccgcgaccg ccgagagcgc gccgccgccg cgcgcggtgc cgtccatctt
     4921 cgtcacgagg acgccgtcga tcccgatcgc ctcgtggaac cggcgggcct gttcgctcgc
     4981 ctgctggccg agagccgcgt cgatgacgag ccaccggtgg tcggcgcgcg cgatatcgtt
     5041 gatgctgatg atctcctcga tcaggttctc ctcgagggcg tggcgcccct gggtatcgat
     5101 gatgatgacc tcgtactgct tccggagctt cgggaggccc tctctgacga tccggagggc
     5161 atccttctcg cccgggtccc cgtagcaggg gatgccgatc cggtcgcaga gggtcttcaa
     5221 ctggtcatag gcgcccggcc ggaaggtgtc ggcgcagatc acgccgaccc tgagcccctt
     5281 gcgctggaag tagcgggcaa gctttgccgt cgtcgtcgtc ttcccgctcc cctgcagccc
     5341 cgccatcagg atagtctggg gcccgagcgc caccgctccc ggcgccccca tcagccggac
     5401 gagctcctgg tagacgatcc gcaggacgtg ctcgcgcacg cccatcccct tcgggggctc
     5461 ctcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngatatgg ccgcgccgcc
     5581 ctttgaatcc atatccgcct tcgtcaggac gacagcatcg gtaccgaccg cccggttgaa
     5641 ctcgccggcc cggatgacgg catcgtttcc tgccaccgcc tcatcgacgt agacgacgag
     5701 gtcgggtttc atgacccgcc ggatcttctc gagctggttc atgaggtttg cccggttgtg
     5761 gaaccggccg gccgtatcgg cgaggacgac atcgatctcg tgcgcctttg cgtactggac
     5821 cgcatcaaag aggacggcgg aagggtcggc gcccgcctgg tgctggatca ccttgatgtc
     5881 caggcggtcg gcatgggtcc ggatctgctc gatcgcgccc gcgcggaagg tgtcgcccgc
     5941 gccgatcacg accgtaaacc cgttcttctg gaggtaatgg ccgacctttg cgaccgtcgt
     6001 cgtctttccg gtcccgttca cgccggtgaa gaggatcttc accggccgct cgtgctctct
     6061 gatatagtca cgaaggtcga ggccgtcgcc aagcaccccg cagagcgcgg agcgcagggt
     6121 cgcgacgacg agatcgtcga ccgatgcgcc gatcttcctg tgccggccca cgaggtcttt
     6181 cttcatccgg gcgatgatct catccgtgac cgggagagcg acgtcgtttt cgaggaggac
     6241 catctcgagc tccatcaggg gctcctcgat atccttctcc tggaggagga tctcccgctc
     6301 ccggacgagg atctttaacc ggttgaaaaa cgttggatcc tcccgtttct cctccctggc
     6361 ggcaggaggc gccagaggtt tccccgggag agggggtgcg ggtgtgatga ccggcggcag
     6421 tgggggttcg acggtcggcg gcggtgcggg ctgctcgacc gccggttgca ccgtggcggc
     6481 ctcctcgata ttcgaggcga actttgtccg tatattctga agtttcttct ttagcgaatc
     6541 aaacattatc ggattccgcc ctgaccagcc tctccctgcc ggatcccccg gtaggccgcc
     6601 tcgatgcggc gggagatctc cgttgcctgc ccctgcaact gctcgactga acctgcgacc
     6661 ttcttgccga ccgcctcgag ttcggttatc cgatcgccga ggtactcgat cgcatccgca
     6721 ctggcacgct ccacggagac atcggccccg atgttcacga ttacgtggcc ggcatcgagc
     6781 acctgtaccc ggagaagggc gccgcccccg atggggagga ggacgacgcc gtccgtgctt
     6841 tcctggaggg cccggagggt ctcgatggcg gcgaccgcct cgatgcgctg ctgctcgatc
     6901 atgccaagct gctgcgtcag gatctcgatc tgctgcccgt actcgtttaa gtacatctgg
     6961 agcgtctgta tctcacgggg atctgcctgg ttcacgttat tcaccagcta ctacgttaac
     7021 cgactcgatg gtgatataac ttctcttcag gcgatgcttg ctcccgatat ccgcaaaaac
     7081 ccactctctg gcctggttct cgtttggtgc ctcgacgacc ttgcggtacg gtttccacgc
     7141 gttgttgatc ctgcacgcgc ccacaacttc aaacgtctgg ttctccatga ctgcttcact
     7201 ccaaaaaccc aaagacttcc tctatccgcc cgagttcaaa cccggtcgta aacgatcctg
     7261 caatgtatcc tttgctgttt gcaaggagac cggttccgac aagaccgctg cccatgttga
     7321 ccgacccaag gccgatcggg aggtcgacca cccgctcgag ggtcgcgatc tccgtctcgt
     7381 tcgtccgggg atgagcgaga atacccttgt ttgtcgcgca tccggccatg ccgacggtct
     7441 tgatgccccc gagcgtgagc cggatcaccg gcacgccgag gaacgacccg atctcttcgg
     7501 caaccccgac atccatgtcg gggtggacgg cggcaacata gtcgtttgcg agtatgacgt
     7561 tgcctgccgc gttcatggac ccctcgagca ggaggacttc gcggtgctcc gcaaggaccg
     7621 cgagctcatc ggcggcggca aggccgctga cgaccaggcc ccggctgttc cccgcaacga
     7681 gagaaccgat gatgccgctc tcctggacgg tggtggccac gatatcgaca gagagttccc
     7741 gcccgagggc ttcggtgaac tccccgggca cccgggggtc cacgatcgcg atatcctcaa
     7801 agacccgggc gtagacgccg atgttcgggt cgcccaagag atcgatcgtc ccggtcatct
     7861 cactcctcgg cgagctcggc ctggacctgc ccgtcctcga acttcatcgc gcggacgcga
     7921 atcttccgcg ggggttttcc actcccgttc tcccagacct tctcgttgat gctccggtcc
     7981 agtttgacgt ccgcactctt catgtgccgt tcgagaaacg cccggatatc cttgatggcg
     8041 gtgttgcccc gcttccaccg gggtgaacgc ttcacctcgc ggagagggat gatatagata
     8101 tgctctttta acgcctctgc cataacctta caccttcaag ctgctccgtc tccagttgcg
     8161 ccgcttcgga tgcgacgaaa ccgcacggtt ggtcctgatc atcacccatg ccggcacgcg
     8221 gcggttctgc tcgcatgcct ttcccagccg gatcttccgg cctttcgtca acttgctcat
     8281 aattgtctca ctcctgtatc cccattcccg gtcgttcgca taccgaccac gagcgactgc
     8341 aggtggccgg ccgggaagaa cggcgccgga tcgatgccgc gggcacggat ggcctcccgg
     8401 atctcctgct cgtaatcgcc gttccctata ctgcccgtct ccccgtaccg gaccacaagc
     8461 agccttcccg cgaggcgttc cacctccggt ttcccgtagc cgaggagcgg ccggacgtag
     8521 gaactgccgg tggtcatctc caggtgctgc accgtggacc gctcgagccg gggaacccgg
     8581 tcttcgcgcc gggtgccgtc gccgacaacc cgatactcac ccgcaagggc ctcgattgcc
     8641 gcccgatgga ccatggtgat cgcgtcgttt gggtaaccgc agacaagaag catatcggcg
     8701 gcttcctcga ggaggtctct tccaagcacc ctccgcctga aggggaggtg taatgcggcc
     8761 gctgcggccc gcaccgccgg aacatcgcga gcgggatcga atacgcaggt attcagttcc
     8821 acgccgtagt cgcgcgcgag catgactgct gcaagcgcgc tgtctttccc tcccgagaag
     8881 agcagacctg cttccattac tttcgcgtaa tcctgaactc tttctttgac ggcgtcaact
     8941 gcgtcagcag ggctttcaac tgatcgtcgg tgatccgctg ccggaccctg ccgctctggg
     9001 caagcaccac cagctgctgt tcgaccgcct ttgcaaactc cggccgggtc aacttgatgg
     9061 tattgagcct ctccctcgct tcaggctcga ggatctccat gagcacgacg cggatctgtg
     9121 actcgatctg ccgctggcgt tctacttcgt cctccatcgc ctgctgatcc atcgcctgcc
     9181 gttgcatctg ttccatcttc cggcggcgga gttctgcgag ttcgtcatct accatcagtc
     9241 atacctccct gatcaatact tcgcgattcc gggtgaaatc ccggaaactt gaggttttac
     9301 gttgtttgca acgttgtcga ggaacgatct gccggccgcg gtgaccgtgc gcccttcgct
     9361 tgtgtgagcg acgtagccgg ccgcctcgag ctgctggaag atcttccgga cgatcgaccc
     9421 gcttccccgc ttgaaccggg acggagccga gcccctgtcg tgtttgccgc cgtagatgga
     9481 gcgcatcctc tgggtcccga cggggccgtc ggtgtagatg cgccggaaga ccgccgccgc
     9541 gcgcacgtac caccagtcgt cgttctcggg cggcatctct ttgtgtaccc ccgtcttcac
     9601 aaaatcggcc cagtcggggg gctgaattgc gggatttttc ttaagttctt ctgctgcctg
     9661 ccggatcaac atatcggcag ggatgtcgta tacagtcgtc atagatgaac ctcactattg
     9721 ctaacagtct ttacatattc gtccagtgat gttttatata tttcgagaat tgcgaccggg
     9781 cgatcgactt cgccgctctg caaggacgaa ggtccgcccc ctgacctcga tgagaaccgc
     9841 accggccgac gccgccacct cctcgggatc gacctcggtg ttcctgagcc accggacctt
     9901 gacgatcttt cgatcctcga gctggcgccg tatctcatcg atcatgaggc tgctgatgcc
     9961 ccgtttcccc acccagacgg tgggcttgat atcctgaaac gactctctgc tcacgaggtt
    10021 cgcctcccga cagggtagcg tgaccggtgc ccgcaggcaa gacaggtgac gatcacgcgc
    10081 ccgcggtaga tccggacccg ggcattcgcg cccgggacca ggtagacgct gcaccggcgg
    10141 cagaaccggc gtttcagcgg ccgatctatc cgcacccggt ggcgcatgcc gatctttcgc
    10201 gcgagttcca cgcaccggtt gctccatgcg gggttatcgg ggtagaactc cgcggcacgc
    10261 gcgaaaagga tggcaatcct ctcgcgggca agcctccgcg agcccgatct tcgtgtggtg
    10321 tctgccatga tccgatccgt tctgccgatt ggtgggtctt acggttagcg actaaaggat
    10381 atatagcagg ccggtgtcag cggacccgca cctttcggcc ggcgacctcc aggcggtcct
    10441 cgcagaagag tttcacggca agcgggaggg ctctgtgctc ctctgcgagg atccggtcgg
    10501 caagcgccga ctcgtcgtcg tccgagagga ccgggacgca tcgctggacg acgatggggc
    10561 ccgtatccat cccctcgtcc acgaggtgga cggtgcagcc cgagaccttc accccgtact
    10621 cgatcgcctg gcgctgcgcg tgcaggccgg cgaacgccgg gagcagggcg gggtggatgt
    10681 tcatcatccg gcccgagaac tcatgcacga tcgcggcccc gaggatccgc atgtagccgg
    10741 caaggacgaa gagatccgcc cggcaacctc gcatcgcggc aaggagcgcc tcctcgtaag
    10801 cggcctttga gggaaaccgc gcataatcga cgaccgtcac cggtatgccg gcggctcttg
    10861 cccgctcgac cgcgtaggcc tgcgggttgt cggtgatgag gccgacacag atcgccggga
    10921 tatccccggc cgcgacggca tcgatgaccg cctgaaagtt cgatcctctt cccgatgcga
    10981 gtgttgcaat ccgtttcttc ctctccatgg acattctctc cttaccggga ctcctccgga
    11041 gcaggcgggg gagcgacgat cagtttcacc ttgacgaggc ggcggccccg catctgcatc
    11101 accaccagcg tgatgttgct ctcctcgagt ttgaccacct cgccgcggcg ggggatgtgc
    11161 cccaggcggt cgatgacgag gccgccgatg ctctcgtagg catccgtcag cgggagggcg
    11221 aggtgcaggt cttcgttgag gtggtccacc catgcccgtg cgtcgaccag gtagacgttc
    11281 tcctcgaccc gttgcacctc gggctcctcc tcgtcgaact cgtccaggat ctcgccgacc
    11341 agctcctcaa gcatatcctc gaccgtcacg atgccggcaa acgacccgta ctcgtcgagg
    11401 acgacggcca tatgctgctt cttcacctgg agttccttca agagttcgtc gatcttcttg
    11461 ctctcgggga cgaagtaggg ctcgtacatc acgtcgcgga tcatcgcgct cgtctgctgg
    11521 cggacgaccg ccgcaaagac gtctttcacg ttcaggagac cgacgacgtt gtcgatctgt
    11581 tcgtggtaga cggggatccg ggagaaaccg gtctcgttga ataaggcgag ggcgccctcg
    11641 atcgtgttcg catcctcgat catcacgacg tcgatccggg gcgtcatcac ctcgcggacc
    11701 gtcgtgtcgc cgaagcgcag gacggagtag agcatatccc gctcctgctc ctcgatcgtc
    11761 ccctcctcct caccgacgtc gatccactcc ttgatctcct cctcggtgac gaccggttcg
    11821 gtcacgccgg gcctgaaggc aaactgccgg ttgatccggt ccgatatcca gagcacgggg
    11881 tagagcgcct ttgagaggta gaggatgggc cgggcgacgc tgagcgcgag gctctcggtg
    11941 tgccgggagg cgtacatctt cggcccgatc tcgccgaaga ccagcatcag gatgaccgtg
    12001 acgccggtcg cgatgccgat accgacgtcg ccgtagatgc cgatcgcgat cgcggtcgca
    12061 agcgatgctg cggcaacgtt tgctatgttg ttcccgatca ggatggtgat caggatggtg
    12121 tcggtcgatc gtttcagtac gtcgagcgct tccgctccct tccgaccctg gtttaagagt
    12181 gcgcggactt tcgcccgggt tatcgatata agggcgacct cagaacttga gaagaag
//
LOCUS       DAOSII010000057        11786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_5438_length_11786_cov_50.138863, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000057 DAOSII010000000
VERSION     DAOSII010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11786
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5438_length_11786_cov_50.138863"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="PLV90_05720"
     CDS             complement(<1..938)
                     /locus_tag="PLV90_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HOF96649.1"
                     /translation="MKKIIVYVRKDGRDRVEEALKGLRYTVSLVQDIYEIVVYTPEEN
                     LDDLVGRLRDVLDLQHDENMIEVSTPDFVVSSLLLHAEQEEGRKEEKTPVEELIDSVK
                     GYAELDLEKVALTSIAGLIALSGLFLDNPVIIIGAMLLSPIIGPIYGFAIYTALGMIR
                     ESLKCLGVLSALLLGVFLLSVMATFLIGSAMPLALTDEITSRLVANPIYTLVAVLLGF
                     VAVLAFNRGTSEVIAGVAIASAIIPPTVVAGLVLVLQPMSLVSSALLVAGQIAGLITG
                     ALVAVTLQKIEPRETRDRTVARRHLRWSVTLIVLLFA"
     gene            1236..2201
                     /locus_tag="PLV90_05725"
     CDS             1236..2201
                     /locus_tag="PLV90_05725"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HOF96650.1"
                     /translation="MEENMFTPGGRKRLQAKSDRENAPYPRGDGKVKLVTPAWLADHM
                     DDADLTIIDTQPNVHDYIQEHIPGAVYLTEGVLRVSNRGFPTSYSPDACIQESFRRAG
                     LKADSPAVVYTGKGVFSGWGDGLAQVMMAYTLAKYGHDTVYILDGGIDGWKSEGKELS
                     QEFPSVEPSGFTVNVRNDYAVGYDEFQKIKDNDDVIVLDARPKAFYEGTSGPWRARGH
                     IPGAISLPWRSLMDDANPALFKSNDELDTILEGHGVDRFRTVICTCGTGREATNEFVL
                     LKWLYRYPNVRLYEGSITEWTRYPDNPVVTGPRAREERTEAAPAR"
     gene            complement(2441..3109)
                     /locus_tag="PLV90_05730"
     CDS             complement(2441..3109)
                     /locus_tag="PLV90_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOF96651.1"
                     /translation="MRLTWLGHACFLLSGSKTILIDPFIPEGTLPVEPDIVAVTHAHA
                     DHLGMTVQLGKKTVAVNEVAKYLAAKGLPVEPMNIGGSITVDGVRFTMTPALHSSWLE
                     NEGIGCYGGVAGGFVVTMDGTRIYHAGDTGLFSDMQLIRDLYWPDVALLPVGGRFTMG
                     PDEAMIAARYIGAPLVIPMHYDTFPAIRQDLEGFKRAIERTTSIRVAVITPGESIEVG
                     REGA"
     gene            complement(3176..3784)
                     /locus_tag="PLV90_05735"
     CDS             complement(3176..3784)
                     /locus_tag="PLV90_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator receiver protein"
                     /protein_id="HOF96652.1"
                     /translation="MAEDTRKQQLLELFTTITNKKTIVEPMRKVHGTLRDRDAVEREI
                     ALIMREVLDQGYFKTKLAPRQLARLVVAYYDGKNDTEIARALGDEKLSKTVARARVRL
                     KLFRDLDFKMPFDRGMMTDLLDSGKTMKEISEELDVSPSTLREYRHVIEQERDTTLDP
                     YLERIRDVMEDRDLSEQMTRGVANDGLSEAIDIAEAIDMGEF"
     gene            4096..5826
                     /gene="glyS"
                     /locus_tag="PLV90_05740"
     CDS             4096..5826
                     /gene="glyS"
                     /locus_tag="PLV90_05740"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOF96653.1"
                     /translation="MAETSDIYEKVMDVARRRGFVWPSSEIYGSVAGFIDYGPLGAML
                     KRNIENLWRSFYVFQEGYYEIECPTVGTEAIFVASGHVKEFADKMVQCPHCGEYLRAD
                     HIAAENGIENAGTLSAAALQAALYELPCPACKECLGEAGVFAFNLMFETTIGPGSQRR
                     GYLRPETAQGIFTDFPRLLRFYRDRLPFGAVQIGKSYRNEISPRQGMIRLREFSQAEA
                     EIFVHPDEKDHPAFYRYADHTAPLLTIARQVENLEPAAITMRAAVDEGVIANEYVAYY
                     IALTHELLLAIGVDPSRLRFRQHLTDERAHYAADCWDAEVYSGRFGWVEIVGIADRTN
                     YDLRSHAAHSGTSMTVFVPYDEPRRVVKRRIVADMGVLGPRFRGKAKAIADALAASDP
                     GEDGARVTVDGEEFFIPADLYQVREEEVEVRGEEVMPHVIEPSYGIDRMIYVALEHSY
                     AEDEVDGEVRKVLRLPPTVAPVQAAVFPLMNRDGLDGIAQAITDRLTRCRILAQYDDS
                     GAIGRRYRRQDEIGTPFAVTVDYDTLEDNTVTVRDRDSMEQVRVPVERLPEVLSALIS
                     GAAAFRELRV"
     gene            5823..8108
                     /locus_tag="PLV90_05745"
     CDS             5823..8108
                     /locus_tag="PLV90_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOF96654.1"
                     /translation="MTSVSHPLIRPESIEERRYQLAIALRALDANTMVVLPTGLGKTA
                     VALLVAASRLYSHPGKVLVLAPTKPLVEQHLRFFKRFLLFREASEPEESDFAMFTGDT
                     PPDDRAKAWEACRICFATPQVIKNDCLAGRYSLADVVLLVVDECHRAVGNYAYVFLAR
                     HYCTTAREPLLLAMTASPGGDRLKVQEVCANLHVEAVETRVETDEDVRPYIHERDIQY
                     VDVYLPEELQAALVALRGLVEARLTRLASLGFQVPKPDKLSIKALNLLNAQIQQRIQA
                     RDPAAFAAASLHAECMKLRHAISLAETQGSEALKLYLARLGAEGASGAGSRASKHLIG
                     DPAYQRLVEVAGGWKEELHPKVPIVCEIVRAQLAAHPDSRIIVFATYRDTVQTLVDAI
                     SASGVACERFVGQASRDAERGLSQKEQIASLSRFREGEFRCLVATSVGEEGLDVPSTD
                     MVIFYEAVPSEIRSIQRKGRTGRSGSGTIVVLVTKGTSDETFRYVSQTRERAMVKGIR
                     DMSVAPVPVPDSISTLTPVPAPAPSIPAATRQADILAFASSGPAIVVDDREMSSRVIE
                     RLHELGASITIERLDLGDYAIGDRILVERKTVQDFVDTLIERDLFGQVGAMADAVPRP
                     VLIVEGGDDLYGVRNLHPNAVRGTLAAITVGMGVTLIRTRDADDTAGMLYVLAQREGG
                     ERGERKVHPKKSHRSVREEQEYALAAFPNIGLKSARLLLEHFGSLKAIFDADAEDLAS
                     VHGVGEKTARGIWELARRPYR"
     gene            complement(8099..8449)
                     /locus_tag="PLV90_05750"
     CDS             complement(8099..8449)
                     /locus_tag="PLV90_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sjogren's syndrome/scleroderma autoantigen 1
                     family protein"
                     /protein_id="HOF96655.1"
                     /translation="MTADKADEVMAEYLLKGGKMLAKSCKVCGYPLFEYRGETRCVVC
                     PLAEPESPLPVPEPAQSPSPAREPPGPVAAEIERTIVHLCERIRSEPRAGECLTLMKA
                     VDRGARALGRLDQR"
     gene            8516..8779
                     /locus_tag="PLV90_05755"
     CDS             8516..8779
                     /locus_tag="PLV90_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0147 family protein"
                     /protein_id="HOF96656.1"
                     /translation="MASPDETIRICIQMLQNISEDSTIPRNIRRVADETKTVLLDNSR
                     SIGLRAATAISMIDEISNDPNMPVHARTRIWELVSQLETVPLD"
     gene            complement(8919..9332)
                     /locus_tag="PLV90_05760"
     CDS             complement(8919..9332)
                     /locus_tag="PLV90_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOF96657.1"
                     /translation="MFHTTIELETHGEGEIIDLTFQVQQAITESAVRMGLVTVFVTGS
                     TAAVTTIEYEPGVLSDLSRALAVIAPDDIPYAHDAAWGDGNGRSHVRAAIVGPSLSIP
                     VIGGRLACGTWQQIVLVELDVRASRRRTVHVTVHD"
     gene            9462..9707
                     /locus_tag="PLV90_05765"
     CDS             9462..9707
                     /locus_tag="PLV90_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96658.1"
                     /translation="MVYRWNDRRDEAVVEMNVPDAGQAICGWLMHALQQAIYDSGPEP
                     AGYFFRELERHNPASLRCFREPEFWPSGGCSANLCGT"
     gene            complement(9861..10925)
                     /locus_tag="PLV90_05770"
     CDS             complement(9861..10925)
                     /locus_tag="PLV90_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOF96659.1"
                     /translation="MTRTALRIIAGKEFSDHLRSRRFHLLFAILAVVAAVGMITGAVQ
                     YTKDLSDYNAVQMIAEGEDDPTLAGNLAGLMRPSILSAYSQMGMLMAGIGVVLGIAMG
                     FDLVTREKESKSLKSLLAYPVFRDEVINGKALGGVGAIALAMGAVLVVSVAVMALFGI
                     VPDLDEFVRILVFGTASFLLVFTFFALALLMSTVSKDSGNALLYSLIVMIVLSSFVPI
                     FAYTPVYSAVFGDPPAQPGTATYSYQQGSSAYMVTTTKVGVRASGDAIDPEALAEYER
                     ASREYTNQKRTITDIVTLVSPTGNYQTILGAASQPAGDNTPGLGSLLGALAYNLIALL
                     AMPAAFFGLAWVRFMREDIR"
     gene            complement(10922..11785)
                     /locus_tag="PLV90_05775"
     CDS             complement(10922..11785)
                     /locus_tag="PLV90_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF96660.1"
                     /translation="MDDLCLEIPAGEVFGLLGPNGAGKSTTILMLVGLIRPTSGACSI
                     GGIEVAAHPIEVKRKIGYMPEDVGFYADRTAAENLDYAARFYGLAEAERKRRVGDLLA
                     LVGLDGVGTTVGGYSKGMRQRLGLARALVNDPEVVILDEPTANLDPRGVLDYRRIVRD
                     LAGRGTTVLVSSHILSEVSKVCTSVGILARGRLVASGDREVLSRAAMQDRVTIDVETG
                     NPMPRFASPEILSAEFSQDACSARIVAESDISTLIAKTLYTEGILLRRLAVEKPDMED
                     ILLSYYTEGTA"
BASE COUNT         2223 a         3765 c         3684 g         2114 t
ORIGIN      
        1 gcaaagagca gcacgatcag ggtgaccgac cacctcaggt gccgtcgtgc gacggtccga
       61 tcccgggtct cccgcggctc gatcttttgc agggtcacgg cgacgagcgc ccccgtgatc
      121 agcccggcga tctgcccggc caccaggagc gccgacgaca ccaggctcat gggctggagc
      181 accaggacaa ggccggccac caccgtcggc gggatgatgg cggaagcgat ggcgacgccg
      241 gcgatcacct ccgatgtgcc gcgattgaac gccagcaccg ccacgaaccc gaggaggacc
      301 gccaccaggg tgtagatggg gttggcgacg agccgcgagg tgatctcatc ggtcagcgca
      361 agcggcatgg cgctcccgat caggaaggtt gccatcaccg agaggagaaa gacgcccagg
      421 agcagagccg agagcacgcc caggcacttc agggactccc ggatcatgcc aagagcagta
      481 tagatggcaa acccgtagat cgggccgatg atgggggaga ggagcatggc cccgatgatg
      541 atgaccgggt tgtcgaggaa gaggccggaa agggcgatca ggccggcgat ggatgtcagc
      601 gccactttct caaggtcgag ttctgcatac cccttgaccg agtcgatcaa ctcctcgacc
      661 ggcgtcttct cctcttttct gccctcttcc tgctcggcgt gcagaaggag cgacgagacg
      721 acgaagtccg gcgtggatac ctcgatcatg ttctcgtcat gctgcaggtc cagcacatcc
      781 cggagcctgc cgaccagatc gtcgaggttc tcctcaggcg tatacaccac gatctcgtag
      841 atgtcctgca cgagcgatac cgtatagcgg agccccttga gagcctcttc tacccgatca
      901 cgcccgtctt tccggacgta tacgatgatc ttcttcatga gtccgtcccg ggaaggacgg
      961 tggttctgcc ggggatcggg cgatcccgga gaacccccga tacatcctcc gccatcttcg
     1021 ctggtctccc gccagtggtc ggtccgggga gaagaacctg gcgatcggca cccgggaccg
     1081 gtgcggggaa cccctgttcc tgcgaggaga gggtcgccgg tgcatgatgg cactcgttgt
     1141 tcccgctccc cgccgggtcc tgcataaggt atataacttc agacctgcac tgaaggccgc
     1201 cctccagtga ggacaccctg cacgaggtga tgggaatgga agagaatatg tttacccctg
     1261 gcggcagaaa acgactgcag gcgaaaagcg accgcgagaa cgccccgtat ccccgtggcg
     1321 acggaaaggt caaactggtc actccggcat ggctcgccga ccacatggac gatgccgatc
     1381 tcacgatcat cgatacccag ccgaatgtgc acgactacat ccaggagcat atccccggcg
     1441 ccgtctacct gaccgaaggg gttctgcggg tatccaaccg cggctttccg acgtcgtaca
     1501 gcccggatgc ctgcatccag gagtcgttcc ggcgcgcggg cctcaaggcc gactcgccgg
     1561 ccgtcgtcta caccgggaag ggcgtgttct ccggctgggg agacgggctc gcacaggtga
     1621 tgatggccta tacgcttgca aagtacggtc acgacaccgt gtacatactc gacgggggga
     1681 tagacgggtg gaagagcgag ggcaaagaac tctcgcagga gttcccgtcc gtcgagccgt
     1741 ccgggttcac cgtaaacgtc cgtaacgact atgccgtcgg gtacgacgag ttccaaaaga
     1801 tcaaggataa cgacgacgtg atcgtgctcg atgcgcggcc gaaggcgttt tacgagggga
     1861 cgagcggccc ctggcgggcg cgcgggcaca tccccggggc catcagcctg ccctggcgaa
     1921 gcctgatgga cgacgcgaac ccggcgctgt tcaagtccaa cgacgaactc gacacgatcc
     1981 ttgaagggca cggcgtcgac cggttcagga ccgtcatctg cacctgcggg accggcaggg
     2041 aagccacgaa cgagttcgtc ctcctgaaat ggctctaccg ctacccgaac gtccggctct
     2101 atgaagggtc aatcaccgaa tggacccggt atccggataa tcccgttgta acgggacccc
     2161 gggcacgaga agagagaacg gaggcggctc ctgcgcggta acccgtgcag gctccgggca
     2221 acgacccgga atctttattt tacagccttt tcagttagaa ataactatat agtagtaccg
     2281 cccaattacc cagagagaga tctggatcca cacaagacac ttcccccctt gacgtcgtct
     2341 tttcgtgtat ccttttctct caaaccgcgc ttaccgcaaa tctcttctgg tatccctcca
     2401 gttaccggcg aggatccgcc gcgccggatg agggcgcccg tcacgcccct tctctgccga
     2461 cctcgatgct ctcgcccggc gtgatgaccg cgacccggat cgacgtcgtc cgttcgatcg
     2521 cccgtttgaa cccctccaga tcctgccgga tggccggaaa cgtgtcgtag tgcatcggga
     2581 tcacgagcgg cgcgccgatg taccgcgccg cgatcatcgc ctcatcgggt cccatcgtga
     2641 aacgcccgcc gacggggagg agcgccacgt ccggccagta aaggtcccgg atgagctgca
     2701 tatccgaaaa aagccccgta tcgccggcat ggtagatgcg ggtgccgtcc atcgtgacga
     2761 cgaaccctcc ggcaacgccg ccgtaacaac ctatgccctc attctccagc cacgacgagt
     2821 ggagcgcggg cgtcatcgta aaccggacgc cgtcgacggt gatgctgccc ccgatgttca
     2881 tcggttctac cgggagaccc tttgctgcca ggtacttcgc cacctcgttg acggcgaccg
     2941 tcttcttccc taactgcacc gtcataccga ggtggtctgc gtgggcgtgc gttacggcga
     3001 cgatatcggg ctctacaggg agtgtaccct ccgggatgaa agggtcgata aggatcgtct
     3061 tcgatccgga gagcaggaaa caggcgtggc cgagccaggt caacctcatg gagaagagat
     3121 cggtctatct cttaaaaaat gttgggagaa aggcaggggg tgagtacgga tagattcaga
     3181 actcccccat atcgatcgcc tcggcgatat cgatcgcctc ggagaggccg tcgtttgcga
     3241 caccgcgcgt catctgttcg gagaggtcac ggtcttccat cacgtctctg atccgttcca
     3301 ggtacgggtc gagcgtggta tccctctcct gttcgatcac atggcgatac tcacgaagcg
     3361 tcgaggggct cacgtcgagt tcctcgctga tctccttcat ggtcttcccc gaatcgagga
     3421 gatccgtcat cattccgcga tcgaacggca tcttgaagtc gaggtcgcgg aagagcttga
     3481 ggcggacccg ggcacgcgcc acggtcttgc tcagtttctc atcgccgagc gccctcgcga
     3541 tctccgtatc attcttaccg tcgtagtacg cgaccacgag tcttgcgagc tgtcgtggag
     3601 cgagtttggt cttgaaatac ccctgatcga ggacttccct catgatcagg gcgatctcgc
     3661 gctcgacagc gtcgcggtcc cggagcgtac cgtgaacctt cctcatcggt tcaacgatcg
     3721 tctttttgtt cgtgatggtc gtaaacagct caaggagctg ctgcttgcgg gtatcttctg
     3781 ccatatccgt gcctctctgt cctgtattcc atcatccgac tctggaggtc attcatgacg
     3841 accccggaca tatatagatt atcccccggc gagggggcgt aacgctccct acattacaac
     3901 tgctaccatt taaaactttc tatataatcc gtgtagggat gcggatcttt ttccgggata
     3961 taacaatatc ttttggtata ttgccggtgg tgaaaaaaat tccacctcgt acagcccctt
     4021 ttcgaagaat tttgatcccg ggcaggggaa gaaccattct ttaatagcac cggcgctcaa
     4081 gtgtactgca ggattatggc cgagacgagc gatatttacg aaaaagtcat ggatgtagcc
     4141 agaagacgcg ggtttgtctg gccctcgtcc gagatatacg ggtcggtcgc cggttttatc
     4201 gattacggcc cgctcggagc gatgctgaag cggaacatcg agaatctctg gcgatcgttc
     4261 tatgtcttcc aggaaggcta ctacgagatc gaatgcccca ccgtcggaac cgaagcaatc
     4321 ttcgtcgcat ccggccacgt gaaagagttc gccgacaaga tggtgcagtg tccgcactgc
     4381 ggcgagtacc tccgcgccga tcacatcgcg gcggagaacg gcatcgagaa cgccggcacg
     4441 ctctcggccg cggctctcca ggcggcgctc tacgagctcc cctgcccggc gtgcaaggag
     4501 tgcctggggg aggcgggcgt ctttgcgttc aacctgatgt tcgagacgac cataggcccc
     4561 gggtcgcagc ggagaggcta cctgcgcccc gagaccgccc agggtatatt caccgatttc
     4621 ccccgcctcc tgcgtttcta ccgtgatcgg ctccccttcg gcgccgtcca gatcgggaag
     4681 tcgtaccgaa acgagatatc cccccgccag ggcatgatcc ggttgcggga gttctcgcag
     4741 gcggaggccg agatcttcgt ccaccccgac gaaaaggatc accccgcgtt ctaccgctac
     4801 gcagaccata cggcgcctct cctcaccatc gcgcggcagg tcgagaacct cgagccggcc
     4861 gcaatcacga tgcgcgccgc ggtggacgag ggcgtgatag caaacgagta cgtcgcctac
     4921 tacatagcgc tcacgcacga gctcctgcta gccatcggcg tggacccatc gcgactccgg
     4981 ttccgccagc acctgacgga cgaacgggcg cactacgcgg ccgactgctg ggacgccgag
     5041 gtttactccg gccggttcgg gtgggtggag atcgtcggga tcgccgaccg caccaactac
     5101 gacctgcgct cgcacgccgc gcattccggc acgtcgatga cggtcttcgt accctacgac
     5161 gaaccgagga gggtcgtgaa acggcggatc gttgccgata tgggcgtcct cggcccccgg
     5221 ttccgcggga aggcaaaggc gatcgcggat gctctcgccg cctcagaccc cggggaggac
     5281 ggtgcccgcg tcaccgtcga cggcgaagag ttcttcatac ccgccgacct ctaccaggtc
     5341 cgcgaggagg aggtcgaggt ccgcggcgag gaggtgatgc cccacgtcat cgaaccgtcg
     5401 tacggcatcg accgcatgat ctacgtcgcc ctcgagcaca gttacgcgga ggacgaggtg
     5461 gacggcgagg tgagaaaggt gctccgcctc ccgccgacgg tggcgccggt ccaggccgcc
     5521 gtcttcccgc tgatgaaccg tgacggcctc gacgggatcg cgcaggcgat cacggatcgg
     5581 ctcacccggt gccgcatcct cgcgcagtac gacgactccg gcgccatcgg caggcgctac
     5641 cggagacagg acgagatcgg gacgcctttt gccgtcaccg tcgactacga tacgctcgag
     5701 gacaacaccg tgaccgtcag ggaccgcgac agcatggagc aggtccgcgt accggtcgaa
     5761 cggctgccgg aggtcctctc cgcgctcatc tccggcgccg ccgcgttccg cgaactcagg
     5821 gtatgacctc tgtctcccac ccgctgatcc ggccggagag catcgaagag cggcgatacc
     5881 agctcgccat cgccctccgg gcgcttgacg cgaacacgat ggtcgtcctc ccgacgggcc
     5941 tcgggaagac ggccgtcgcg ctcctcgtcg cggcatcccg tctctactcc cacccgggaa
     6001 aggtgctcgt gcttgcaccc acaaaacccc tggtcgagca gcacctccgt tttttcaaac
     6061 ggtttctcct cttccgggag gcttccgagc cggaagagtc cgactttgcc atgttcaccg
     6121 gggatacgcc ccccgacgat cgggcgaaag cctgggaggc gtgccggatc tgttttgcca
     6181 cgccgcaggt gatcaagaac gactgcctgg ccggcaggta cagcctcgcc gacgtcgtcc
     6241 tgctggtcgt ggacgagtgc caccgggcgg tgggaaacta cgcctacgtc ttccttgccc
     6301 ggcactactg cacgacggca cgcgagcccc tgctgctcgc gatgaccgca tcccccgggg
     6361 gggaccggct gaaggtgcag gaggtctgtg caaacctcca tgtcgaggcg gtcgagaccc
     6421 gggtggagac cgacgaggat gtccggccct acatccacga gcgggacatc cagtacgtcg
     6481 acgtctacct tcccgaagaa ctgcaggcgg cactcgtcgc cctccggggg ctcgtcgagg
     6541 cgcgtctcac ccgtcttgcg agcctcggct tccaggtccc gaagccggac aaactctcga
     6601 tcaaggcgtt gaaccttctc aacgcgcaga tccagcagcg catacaggcg cgggaccctg
     6661 ccgccttcgc cgcggcgtcg ctgcacgccg agtgcatgaa actccgccac gccatatcgc
     6721 ttgccgagac gcaaggaagc gaggcgctca aactgtatct cgcccggctc ggcgcggaag
     6781 gggcctcggg cgccgggagc agggcaagca agcacctcat cggcgatccg gcctaccagc
     6841 gccttgtcga ggtcgccggc ggctggaagg aagagctcca ccccaaagtc ccgatcgtct
     6901 gcgagatcgt gcgggcgcag cttgcggcgc acccggacag ccggatcatc gtctttgcca
     6961 cctaccgcga caccgtccag acgctcgtcg acgcgatatc ggcgagcggg gtcgcctgcg
     7021 aacggttcgt cgggcaggcg tcgcgggacg cggagcgggg gctctcgcag aaagagcaga
     7081 tcgcgagcct ctcccggttc cgcgagggcg agttcaggtg cctggtcgcg acatcggtgg
     7141 gcgaggaggg gctcgatgtc ccctcgacgg acatggtgat cttttatgag gccgtgccct
     7201 cggagatccg gagcatccag aggaaaggga ggaccgggag gagcggcagc ggcacgatcg
     7261 tcgtcctggt gacgaagggc acgtccgacg agacgttccg ctacgtgagc cagacccggg
     7321 agcgggcgat ggtaaagggg atcagggata tgagcgttgc acctgttccg gttcccgact
     7381 ccatttctac tctcaccccg gttcccgccc ccgcgccgtc gataccggcc gccacccggc
     7441 aggccgatat cctcgcgttc gcctcctccg ggccggcgat cgtcgtcgac gaccgcgaga
     7501 tgtcgtcgcg ggtcatcgag cgcctgcacg agctcggggc gtcgataacg atcgagcgcc
     7561 tcgaccttgg cgactacgcc ataggcgacc gtatcctcgt cgagagaaag accgtgcagg
     7621 acttcgtgga caccctcatc gagcgggacc tcttcgggca ggtaggagcc atggccgacg
     7681 cggtgccgcg gccggtcctg atcgtcgagg ggggagacga cctgtacggt gtgcggaacc
     7741 tccacccgaa cgccgtcagg ggcacgcttg ccgccatcac ggtcggtatg ggggtcacgt
     7801 tgatccgcac gcgggatgcg gacgacaccg ccgggatgct ctatgtcctc gcacagcggg
     7861 aggggggcga gcgaggagag cgcaaggtgc acccgaagaa gtcccaccgc tcggttcgcg
     7921 aggagcagga gtatgcgctc gcggcgttcc cgaacatcgg cctgaagagc gcccggctcc
     7981 tccttgagca cttcgggtcg ctcaaggcga tatttgatgc cgatgcggaa gaccttgcat
     8041 cggtgcacgg tgtcggagaa aagaccgccc gcgggatatg ggagcttgcc cgccggcctt
     8101 accgctgatc cagccttccg agcgcccgcg ctccccggtc taccgccttc atcagggtca
     8161 ggcactcgcc ggcccgcggt tcgctcctga tgcgctcgca gagatggacg atcgtccgct
     8221 cgatctccgc ggcgaccgga cccggtggtt cccgggcggg tgacggcgac tgcgccggtt
     8281 cgggtacggg aagaggactc tccggttccg cgagcgggca gacgacgcac cgggtctctc
     8341 cccggtactc aaagaggggg tacccgcaga ccttacagga ttttgcaagc atctttcctc
     8401 ctttcaggag atactcggcc ataacttcgt cagccttatc cgccgtcatt ccatcaaact
     8461 cccttaatca acctatatat gtctggatgc cattaataat agaggggtag ctctcatggc
     8521 aagtccagat gaaacaatac gaatctgcat ccagatgttg cagaacatca gcgaggattc
     8581 cactattcca cgtaatatcc ggcgggtcgc agatgagacg aaaacggtcc tcctggataa
     8641 ttcccggagc atcggcctcc gcgccgcaac cgcgatctcg atgatcgacg agatcagcaa
     8701 cgatccgaac atgccggtcc acgcacggac ccgcatctgg gaactggtat cgcaactgga
     8761 gacggtacct ctcgattaac ctgctcattc ttctcgaaac gactccggca gaaccaggat
     8821 agagcgatac tatttgtggt tgcaaacgca gccgtatcac tctacccgcc gtgtgtcttc
     8881 acgcaccccg ggtcgcgcat ctctcctttt tccggcgctc agtcgtggac ggtgacgtgg
     8941 accgttctcc tgcggcttgc cctgacatcg agctcgacga ggacgatctg ctgccaggtg
     9001 ccgcacgcga gcctgccccc gatgaccggg atggagagcg acggcccgac gatagccgcc
     9061 ctgacatggg accggccgtt gccgtccccc catgccgcat cgtgcgcata ggggatatcg
     9121 tccggggcga tgaccgcaag cgcccgggaa aggtcggaga gcacgcccgg ctcgtactcg
     9181 atggtggtga cggcggcggt cgagcctgtg acgaagacgg ttacaagccc catccggacg
     9241 gcgctctcgg tgatcgcctg ctgtacctga aacgtgaggt cgatgatctc tccctcccca
     9301 tgcgtctcca gctcgatcgt cgtatggaac atgatacttc ctgatgatcc ctgcacccgg
     9361 ataaacctcc tgttccgacc ggggccggca caatgcaggg ggccgggcgg gatgcccgca
     9421 aacgctttac cggtgaggcg gggctctcct tcccggttga tatggtttac cgctggaacg
     9481 acaggaggga tgaggcggtc gtcgagatga atgtgcccga tgccggccag gcgatctgcg
     9541 ggtggctgat gcacgccctg cagcaggcga tatacgactc cggtcccgag cctgcaggct
     9601 atttcttcag ggagcttgag cggcacaacc ccgcatcgct caggtgcttc agagaaccgg
     9661 agttctggcc ttccggagga tgttcggcga acctctgcgg gacataagca cctgcaggcc
     9721 cgcaccgccg acatccccgg gagaacagcc gttcgccgtt catctcgatg caacggcgaa
     9781 ctctcttccg ccgataatcg agaccgccgt cgccgtttat ccggcaaaaa agagttttcc
     9841 cggccatgaa tcggatccgg tcaccggata tcctcccgca tgaaccggac ccatgccagc
     9901 ccgaagaagg ccgcgggcat cgcgagcagg gcgatgaggt tataggcaag ggcgcccagg
     9961 aggcttccga gtcccggggt attatcccct gcgggttgcg atgcggcacc gagaatcgtc
    10021 tggtagttgc cggtcggcga gacgagcgtg acgatatcgg tgatcgtccg tttctggttc
    10081 gtatactctc ttgacgcccg ctcatactcc gcgagggctt ccgggtctat ggcatcgccg
    10141 gatgcccgaa ccccgacttt cgtcgtcgtc accatatagg ccgacgaacc ctgctggtag
    10201 gagtaggtgg ccgtgcccgg ctgggcgggg gggtcgccga agacggccga gtagaccggg
    10261 gtgtacgcga agatcgggac gaacgacgag aggacgatca tcacgatcag ggagtacagc
    10321 agggcgttcc cgctgtcttt cgagaccgtc gacatcagga gggcgagggc aaagaacgtg
    10381 aagacgagga ggaacgacgc cgtcccgaag accaggatgc ggacgaactc gtcgaggtcc
    10441 gggacgatgc cgaacagggc catgacggca acggagacga ccaggacggc ccccatcgcg
    10501 agcgcgattg caccgacgcc cccgagggcc tttccgttga tcacttcgtc ccggaagacc
    10561 gggtaggcga gcaggctttt caaggatttg ctctctttct cccgggtcac caggtcgaat
    10621 cccatcgcga tcccgagcac caccccgata ccggccatca gcattcccat ctgggagtat
    10681 gccgagagga tggagggtct catcagcccg gcaagattgc cggcgagcgt cgggtcgtct
    10741 tcaccttccg cgatcatctg gaccgcgttg tagtcggaga ggtctttggt gtactggacc
    10801 gccccggtga tcatgccgac ggcggcgacg acggccagga tcgcgaagag cagatgaaac
    10861 cgcctgctcc ggaggtggtc ggagaactct ttgcccgcga tgatgcgcaa ggccgttcgc
    10921 gtcatgcggt cccctccgta tagtacgaga gcaggatatc ctccatgtcg ggtttctcga
    10981 ccgccagccg ccggaggagg atgccttcgg tgtacagggt ctttgcgatg agagtgctga
    11041 tatcgctctc cgcgacgatc cgggctgagc aggcgtcctg cgagaactct gcggagagga
    11101 tctccgggct tgcgaaccgg ggcatcgggt tcccggtctc gacgtcgatc gtcacccggt
    11161 cctgcatcgc ggcgcgcgag agcacctccc ggtcgccgga cgcgacgagc cttccgcgtg
    11221 cgaggatccc gacggaggtg cagactttgc tcacctccga gaggatgtgc gaggagacga
    11281 gcacggtcgt cccccggccg gcgaggtccc tgacgatcct gcggtagtcc agcacccccc
    11341 gggggtcgag gttcgccgtg ggttcgtcga ggatgacgac ctccggatcg ttgacgagcg
    11401 cccgggcgag gccgagacgc tgccgcattc ctttcgagta gccgccgacc gttgtcccga
    11461 cgccgtccag cccgacgagg gcgaggaggt cgccgacccg ccgtttccgc tcggcctccg
    11521 ccagcccgta gaacctcgcc gcatagtcga ggttctctgc ggcggtgcga tcggcgtaga
    11581 acccgacgtc ttcgggcatg tagccgatct ttctcttcac ctcgatcggg tgggcggcaa
    11641 cctcgatccc gccgatggag cacgcgcccg acgtcggccg gatgagcccg acgagcataa
    11701 gaatggtggt gcttttgccg gcgccgttcg ggccgagcag cccgaagacc tcgccggcgg
    11761 ggatctcgag gcagaggtcg tccacg
//
LOCUS       DAOSII010000058        11700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_5484_length_11700_cov_48.640532, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000058 DAOSII010000000
VERSION     DAOSII010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11700
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5484_length_11700_cov_48.640532"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            318..803
                     /locus_tag="PLV90_05780"
     CDS             318..803
                     /locus_tag="PLV90_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96661.1"
                     /translation="MLDEILHIMAAAIISWILFVTVDIFFKLPETGGVSGASAIARDI
                     EAGGGALSGGTMMGNIVCSPDASAGTLLAACGVYVAGIPGGLAAAVMVYVGNRICHDP
                     GYAGTTGAVLATFVVYAFTLAGFAATDFIAGMVIAILTIQGLSHAHASRLLARLWRVR
                     Q"
     gene            800..1039
                     /locus_tag="PLV90_05785"
     CDS             800..1039
                     /locus_tag="PLV90_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2109 family protein"
                     /protein_id="HOF96662.1"
                     /translation="MIALYVVAAIAVFAAIRVIVEKNTGRKLPYANVLGFAVSGAVVL
                     LIDHPLALVAAAAYFVGSTLESNAIASTYAGGVKR"
     gene            1036..1266
                     /locus_tag="PLV90_05790"
     CDS             1036..1266
                     /locus_tag="PLV90_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2108 domain-containing protein"
                     /protein_id="HOF96663.1"
                     /translation="MILILEAAFGFIALVGAISAALIRDSYGKLISLGVLVAGILPFI
                     VDRGYIDVAITVALIAPIATIFVLMVARREEA"
     gene            1263..1502
                     /locus_tag="PLV90_05795"
     CDS             1263..1502
                     /locus_tag="PLV90_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2107 family protein"
                     /protein_id="HOF96664.1"
                     /translation="MNAIFALGLAILVIGVLSVAFVRPKTYVARLINLEIPAWGLLLI
                     MLTYDEALAILTFVAVTAISTFVIVRLLSWRDASC"
     gene            1496..1951
                     /locus_tag="PLV90_05800"
     CDS             1496..1951
                     /locus_tag="PLV90_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2106 family protein"
                     /protein_id="HOF96665.1"
                     /translation="MLIITRISRFISNFQNLVALFAGACIIISILGILSLPLAFEENQ
                     LYPKSDLDPTSGLYPYNRGGVPFGPTEIVCQYPENSPYAGYVTTYLTPLSQYLADHTL
                     YIGTTIVAHPGGIIDEILYNTRGLDTVIETSILFAAFAIASYLYRRKQE"
     gene            1948..2547
                     /locus_tag="PLV90_05805"
     CDS             1948..2547
                     /locus_tag="PLV90_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2105 family protein"
                     /protein_id="HOF96666.1"
                     /translation="MIPDYSIGLLVAFAAAGLAFYALMRETDGLHRLLLTDLIEVVTL
                     AFIALIGTDLAEALILPGLVVGVSELIALSEIYRIKEGYTLPPGPGLQIEVMESAPGI
                     LAALLVIYGIVLSGFSGGAVAGLGVIFYFLCRDHHERFELIETASGYSWVFWIVAFFV
                     FMVLPQYWFFAVMLAGSAILVKVMAKMSLIGTMRGGPNA"
     gene            2540..3193
                     /locus_tag="PLV90_05810"
     CDS             2540..3193
                     /locus_tag="PLV90_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96667.1"
                     /translation="MPDIGGADLFVLEFGDILHYYTPYTATLFILAAIFTGLVIVSRP
                     EGQIDIAFGGDAYYAKETAISEMRFRRFMAIACGLATMGAMGSGDLFNFTLFASLVGI
                     TNVGIVAAVKNRHVLDAAYEYGIVAMVATLPLFGGAALILATTGTLSIPALAAGGFAV
                     PLIGRGLLALGVIGEGMAPFYAAKAEMFRAPGAPYVIMCSLSSLLIFLRVVEIVITI"
     gene            3199..3402
                     /locus_tag="PLV90_05815"
     CDS             3199..3402
                     /locus_tag="PLV90_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96668.1"
                     /translation="MKKTTVTSGLAASVIAIAAAWGLLAIGLIPLIAAEAVTVIAFPV
                     LVLLLGLWWRAPGGEEDIPFIGY"
     gene            3404..4249
                     /locus_tag="PLV90_05820"
     CDS             3404..4249
                     /locus_tag="PLV90_05820"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOF96669.1"
                     /translation="MIEYLVFAAAFGLLLLGLHRKVIARIQMRPGPPVWQEILHALKF
                     SFKSTWIPKTASSTLYVGVVLVGIGIWSAALFVVLAGESILILFAIYMLHKVVEHGFG
                     LSSGSPYGKFGGVRSVISAASEIPLFVTIAAIYLFTGSLLLRDIVAYQGVHGPLLLIV
                     PPVAIAMYLVILSKMPYGPFSIVESKELVSGYKTEHFGVWRAGISVIDGTKTFVLLYA
                     FVAIFIGAVPFWAALIIMTLILVSLSFVCAVTPMLSPFDSVTIQGLVTGLMLVYVGYL
                     WWVMP"
     gene            4246..4488
                     /locus_tag="PLV90_05825"
     CDS             4246..4488
                     /locus_tag="PLV90_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96670.1"
                     /translation="MIRELVLVGGVIYIVIALVQWFENQTVQGEALVFVGALVALSLI
                     HRASDEERLKQLEMAGLWLMIALFAGYAIAKAGGIL"
     gene            4485..4874
                     /locus_tag="PLV90_05830"
     CDS             4485..4874
                     /locus_tag="PLV90_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1959 family protein"
                     /protein_id="HOF96671.1"
                     /translation="MTRYLYEKDLRPMKYTILTSTKHDRTVRAIAHRLGMADAKLRMA
                     MIRRLDMSLLENLESRWEMGQKHADGGDGVAGELGCELFTRFIPLLDTETMQSIYDET
                     TTMIQNGLAVDDAITRGRERIRKAVLR"
     gene            4871..5326
                     /locus_tag="PLV90_05835"
     CDS             4871..5326
                     /locus_tag="PLV90_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96672.1"
                     /translation="MSILQDIKNAVRSRSIHVAYVDVGSCNGCDIEVLACLAPRYDIE
                     QYGIYVHNNPREADVLLVIGSVTPQWVDKLRDIWDKIPEPKVAVAIGNCPISGCVYAR
                     RGTLTTPPVENYIPIAASVPGCPPRPTEILSAILSVAPLIFKDYEEKQP"
     gene            5323..6411
                     /locus_tag="PLV90_05840"
     CDS             5323..6411
                     /locus_tag="PLV90_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOF96673.1"
                     /translation="MKKTVDVSIPLGPVHPCWKEPVRLKCETAGERVLRVEVELGYMK
                     KGIERIMRGRPWQEVMFLAERVCGICSVIHNMVFIETMEEISDIAVPPRAAYLRVIVN
                     ELDRIASHILANFSYCYTIEHETLAMYLLNTREIVLDNLERITGSRVNTAYMIPGGVR
                     FDLLPEDAVTVLADLDKVEEEMRRHTRIFETGPLIALRSKGIGVMDREEAIRSHTVGP
                     TARASGVPDSDLRLRHPTYQALGFEQITRTEGDNFARIMVRFQEIFQSIDLIRKCVTT
                     LPDGPIRGGGLCRAGEGSHSGEAPRGELTYTIKTDEYGRVLDIAIRTPSIMNIEACAH
                     AMIADAASIADVTSTFISSDPCVACNER"
     gene            6415..7023
                     /locus_tag="PLV90_05845"
     CDS             6415..7023
                     /locus_tag="PLV90_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOF96674.1"
                     /translation="MRSIIYYVREFLRPEWLKKFFFAKTAPLATPSYFKDYPSLTEKE
                     CTGCFACMMICPAPGGIVVLAKKDEWVPTVYPGHCIRCGLCVEACPEGVLTSGRILDV
                     TRRDKTAFSSWYRLEVEDALCMRCGNCCVSCPVNRQVDPQLAGTGTSSSDEVIMRIEG
                     GRVRILHEEKCTGCKTCEINCPNSAIRVARMVEGVQGEEAEA"
     gene            7020..7409
                     /locus_tag="PLV90_05850"
     CDS             7020..7409
                     /locus_tag="PLV90_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HOF96675.1"
                     /translation="MNFLMITGRTVNQGVTVENKTSAAYAAETSTCFMHEFDMLELGL
                     ADGDPIRVTGPCGEVVMRAIGSLEVEMGSIFVPYGPYANHIIAAGTRSTGMPDFKSHE
                     VTIEPTDEEPKTVHRLMEDLGGLAYDR"
     gene            7399..8685
                     /locus_tag="PLV90_05855"
     CDS             7399..8685
                     /locus_tag="PLV90_05855"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HOF96676.1"
                     /translation="MIVKDVVCPFCGCLCDDIEVEVEDNRVVAVTNACELGMTKLMGE
                     ERMKNPILRDGNTWKDITYDEAIRYAADMLLSAERPLLYGWSGTHGEAQCVGVHMAEL
                     VHGVIDNTSSVCHGPSILAIQEVGHPGCTLGVVKNRADLVIYWGSNPIDAHPRHLSRY
                     TRYAEGFFLENAFRDRKVIVVDIRKTETANVADEFMQIKPGGDYAVLSALRAIVRGKT
                     ETIPRTVAGVTREQLFRVVNLCKNAKFGAIYFGLGLTMTPGKYKNIRNAIELTSELSR
                     HTKFTISAMRGHYNVYGSNEVNTWMTGYPFGIDYSRGISFYNPGETTAVDILARKECD
                     AALIVGSDPAAHFPRRCLEHLADIPVVQLDPYPNATTAFCNVQIPVAVTGIDAEGTAY
                     RMDGVPIRTRRVLSTGYPSDEEVLTRMYALMQGARS"
     gene            8682..10382
                     /locus_tag="PLV90_05860"
     CDS             8682..10382
                     /locus_tag="PLV90_05860"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HOF96677.1"
                     /translation="MSELLIRNACVIDPLRGINAETMDIAIRDGRIVEEVSAAAEVLD
                     ARGMLTLPGGIDSHTHICGTKVNFGRYMSPEDMRAGRTPRRGPMHATSGYSVPTTYGN
                     SYRYSAMGYTTLLEGAMAPLEARHTHEEFTYTPLQDTMANTLFDGNWGIMEAIHDGDT
                     KRVAAIIAWTLTAAKGFGVKLTNPGGTEAWQWGKNVSCIRDPVPYFEVTPAEIIRSII
                     EANELLRLPHSVHLHCNNLGTPGNYTCTLGSLGLVPDLNEKRQTLYATHVQFHAYGGS
                     GWKDFCSKSEPIARFVNMRPQIVIDMGQVMFGRTTTMTADGPMEFNLYRLHHDKWSNH
                     DVELETGSGIIPVLYRRKNLVNSIMWAIGLELALLTKSPWQCMLTTDNPNGAPFVRYP
                     EIIALLMSKRYRDAEFARIHPDTGGRVPLPAIERELDWHEIAVMTRAGQARALGIQDL
                     GKGYLGPGAEADVAIYPIRIDETDPSVDYQKVIEAFSRTEYTIKRGRVVSRRGECLVD
                     GSNATFWVRAKVSDAYDMGKDPDFIEKFDRYYTVRMRNYPVQEAYLNRNRCIETEAAI
                     "
     gene            10379..11149
                     /locus_tag="PLV90_05865"
     CDS             10379..11149
                     /locus_tag="PLV90_05865"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HOF96678.1"
                     /translation="MRVTLAMKPAERSFIPIEAEAIVPKNFLAGTDISVWRGNRELRL
                     DEVFSVSVEGDADRPEDVEVVLSGDETFRLKRVGEYMDGGKIVVLGGIGMHCGNFMSG
                     GTIEIHGNADGWLGREMAGGTILCRGDAADYCASGYRGGRKGMTGGTVEVFGRAGDFL
                     AESLAGGTVIVHGGAGDMAGAEMHGGTLIVHGDCSRPAANMKAGSCYVYGTAHGMLPT
                     FKRIGAVQHDGRTLIQFTGDIANRGKGNLFVKDYRYLD"
     gene            11149..11298
                     /locus_tag="PLV90_05870"
     CDS             11149..11298
                     /locus_tag="PLV90_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PriA"
                     /protein_id="HOF96679.1"
                     /translation="MVRHACGFDAPIHCKRCGRPLESNERKGLFCPHCGRRVSIVCPG
                     CGRLW"
     gene            complement(11299..11565)
                     /locus_tag="PLV90_05875"
     CDS             complement(11299..11565)
                     /locus_tag="PLV90_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1922 domain-containing protein"
                     /protein_id="HOF96680.1"
                     /translation="MYLIIRCPGCRTFTYVDRYQRWRLCPMCGEAINVGRAPVYLDVD
                     DFLDAERVVEQLESYLHRTGKKDLSEQDIRQLRTQYTEWVKNRV"
BASE COUNT         2179 a         3764 c         3627 g         2130 t
ORIGIN      
        1 cggcaacccc cgcgcgtata gatatcgacg ccgggagaat tataactgtc ccccggccgc
       61 ggcgcgcacc gcgcctgcag agttatcccg ggcggtacac ggcatccacg caccccggca
      121 gatcgccgat agcagcccgg atgtcatgca cgggaagtta tattgtacat gaaactcatt
      181 gaatagtata gaaaattaac cctggaccac cttgtccggg gtgtgccggg agtagattcc
      241 ggccaaatca tgcccggatg ttaacaacac aacatttatt caattgatcg accgtatttt
      301 tcattagttg aacgagaatg ctggacgaga tcctccacat catggcggcg gcgatcatca
      361 gctggatcct cttcgtaacc gtcgatatct tcttcaaact gccggagacc gggggcgtca
      421 gcggggcgtc ggccatcgcg cgggatatcg aggccggcgg gggcgccctt tccggcggca
      481 ccatgatggg gaacatcgtc tgctcccccg acgcctcggc aggtaccctc cttgccgcct
      541 gcggcgtcta cgtcgccggc atccccggcg ggcttgccgc ggccgttatg gtttatgtcg
      601 gcaaccgcat atgccatgat cccggctacg cagggaccac cggcgcggtc ctcgccacgt
      661 tcgtcgtcta cgccttcact ctcgccgggt ttgccgccac cgacttcatc gcagggatgg
      721 tgatcgccat cctcaccatc caggggctct cgcacgccca cgcgagccgg ctgcttgcac
      781 ggctctggag ggtccggcag tgatcgcgct ctatgttgtc gccgcgatag ccgtctttgc
      841 cgccatacgg gtcatcgtcg agaagaacac cggccggaaa ctgccttacg caaacgtcct
      901 cggtttcgcc gtctccgggg cggtcgtcct cctgatcgat catcccctcg ccctcgtcgc
      961 ggcggcggcc tacttcgtcg gctccacgct tgagtcaaac gccatcgcaa gcacctacgc
     1021 cggaggggtg aagcgatgat cctcatcctt gaggccgcgt tcgggttcat agcactcgtg
     1081 ggcgccatct ccgccgccct catcagggac tcctatggaa aactcatatc gctcggtgtc
     1141 ctcgtcgccg gcatactgcc gttcatcgtg gaccgcggct acatcgacgt cgccatcacg
     1201 gtcgccctga tcgcccccat cgccacgatc ttcgtcctca tggtcgcaag gagggaggaa
     1261 gcgtgaacgc catattcgcc ctcggcctcg ccatcctcgt catcggcgtc ctctccgtcg
     1321 ccttcgtgcg gccgaagacc tacgtcgccc ggctgatcaa cctcgagata ccggcatggg
     1381 gcctcctcct catcatgctc acgtacgacg aagccctcgc catactgacg ttcgtcgccg
     1441 tcaccgccat cagcacgttc gtcatcgtgc gcctcctctc ctggagggat gcgtcgtgct
     1501 gatcatcacc cggatatcgc ggttcatctc aaacttccag aacctcgtgg cgctctttgc
     1561 cggggcatgc atcatcatat cgatcctcgg catcctctcc ctcccgctcg ccttcgagga
     1621 gaaccagctc tacccgaagt cggatctcga ccccacaagc gggctctacc cctacaaccg
     1681 gggaggggtg ccgttcggcc cgacggagat cgtctgccag tatcccgaga actcgcccta
     1741 cgcgggctac gtcacgacct acctgacacc gctctcgcag tatctcgcgg accacacact
     1801 ctacatcggc acgacgatcg tcgcccaccc cggcgggatc atcgacgaga tcctctacaa
     1861 cacgcggggg cttgacaccg tcatcgagac gagcatcctc tttgcggcgt ttgcgatcgc
     1921 atcgtatctc taccggagga agcaggaatg atccccgact actcgatcgg ccttctcgtg
     1981 gcgttcgcgg cggcaggatt ggccttctac gcccttatgc gggagaccga cggcctccac
     2041 cgcctcctcc tcacggacct catcgaggtc gtcaccctcg ccttcatcgc gctcatcggg
     2101 accgaccttg ccgaggcgct catcctcccc ggccttgtgg tgggagtctc ggagctgatc
     2161 gccctctccg agatctaccg gatcaaggaa ggctacaccc tgccgcccgg ccccggtctg
     2221 cagatcgagg tgatggagag cgctccgggc atactcgccg cgctcctggt catatacggc
     2281 atcgtcctct ccgggttctc cgggggcgcc gtcgccggcc tcggcgtgat cttctacttc
     2341 ctctgccgcg accatcacga gcggttcgaa ctgatcgaga cggcaagcgg ctactcttgg
     2401 gtcttctgga tcgtggcatt cttcgtcttc atggtcctcc cgcagtactg gttcttcgcg
     2461 gtcatgctcg cgggctcggc aatcctcgtc aaggtcatgg cgaagatgtc gctcatcggc
     2521 accatgcggg gtggtccgaa tgcctgatat cggcggggcc gacctcttcg tgctggagtt
     2581 cggtgacatc ctccactact acacccccta caccgcgacg ctcttcatcc tcgcggcgat
     2641 cttcacgggg cttgtcatcg tgagccggcc cgaagggcag atcgacatag cgtttggcgg
     2701 ggatgcctac tacgcaaagg agacggcgat cagtgagatg cgcttccggc ggttcatggc
     2761 gatcgcctgc ggccttgcga cgatgggcgc gatggggagc ggggacctct tcaacttcac
     2821 gctctttgcc tcgctcgtcg gcatcacgaa cgtcgggata gtcgcggcgg tcaaaaaccg
     2881 tcacgtcctc gatgccgcgt acgagtacgg catcgtcgcc atggtcgcga cgctgccgct
     2941 ctttggaggc gcagcgctca tccttgcgac gacgggcacg ctctcgatac ccgcgcttgc
     3001 ggccggcgga tttgccgtcc cgttgatagg caggggcctc cttgcactcg gcgtcatcgg
     3061 agaggggatg gcgccgttct acgcggcaaa agccgagatg ttccgggcgc ccggcgcccc
     3121 ctacgtcatc atgtgctccc tctcgtcgct gttgatcttc ctccgcgtcg tggagatagt
     3181 cataaccatc tgataaccat gaagaaaacg acggttacat ccggactcgc ggcatcggtc
     3241 atcgccatcg ccgccgcatg ggggctgctc gcgataggcc tcataccgct cattgcggcg
     3301 gaagcggtca ctgtgatcgc gttcccggtg ctggtgctcc tcctcgggct ctggtggcgg
     3361 gccccgggag gggaagagga tataccgttc ataggatact gacatgatcg agtacctggt
     3421 atttgcagca gcgttcggcc tcctcctgct cgggctccac agaaaggtca tcgcacggat
     3481 acagatgagg ccgggccccc ctgtctggca ggagatcctc cacgccctga agttctcgtt
     3541 caagagcacc tggataccga agacggcgag cagcacgctc tatgtcgggg tcgtcctcgt
     3601 cgggatcggc atatggagcg cggcgctctt cgtcgtcctt gccggcgaga gcatcctgat
     3661 cctctttgcg atctacatgc tccacaaagt cgtggagcac gggttcgggc tctcgtcggg
     3721 ctcgccctac ggcaagttcg gcggcgtccg gtcggtcata tccgcggcat ccgagatccc
     3781 gctcttcgtg acgatcgcgg cgatctacct cttcacgggg tcgctcctcc tcagggatat
     3841 cgtcgcctac cagggagtcc acgggccgct cctcctgatc gtgccgcccg tcgcgatagc
     3901 catgtacctc gtcatactct cgaagatgcc ctacggcccc ttctcgatcg tcgagagcaa
     3961 ggaactcgtg agcggttaca agaccgagca cttcggcgtc tggcgggccg ggatcagcgt
     4021 catcgacggg acgaagacgt tcgtcctcct ctacgccttc gtcgcgatct tcatcggggc
     4081 cgtgccgttc tgggctgcgc tcatcatcat gaccctgatc ctcgtctcgc tctcgttcgt
     4141 ctgcgcggta acacccatgc tctcgccgtt tgacagcgtc accatccagg ggctcgtcac
     4201 cggtctgatg ctcgtctatg tcggctacct ctggtgggtg atgccatgat ccgcgaactg
     4261 gtgctggtcg gcggggtgat ctacatcgtc atcgcactgg tgcagtggtt cgagaaccag
     4321 accgtccagg gcgaggcgct ggtctttgtc ggggctctcg tggccctctc cctcatccat
     4381 cgcgcctcag acgaagaacg gctcaaacag ctcgagatgg cggggctctg gctgatgatc
     4441 gccctctttg caggctacgc catcgcaaaa gcgggaggga tcctatgacc cggtatctct
     4501 acgagaaaga cctccggccg atgaagtaca ccatcctcac gagcacgaag cacgaccgga
     4561 cggtcagggc gatcgcccac cgcctgggca tggccgacgc gaaacttcgc atggcgatga
     4621 tccggcggct cgacatgtcc ctgctcgaga acctcgagtc ccggtgggag atggggcaga
     4681 aacacgcgga cgggggcgat ggagtcgcag gagaactcgg ctgcgaactc ttcacccggt
     4741 tcataccgct cctggataca gagacgatgc aatcgatata cgatgagaca acgacaatga
     4801 tacagaacgg actggcagtc gacgacgcga tcacgcgagg cagagaacgg atccggaagg
     4861 cggtcctccg atgagcatcc tccaggatat caagaacgcc gtccggtcac gatcgatcca
     4921 cgtcgcctac gtcgacgtcg ggtcgtgcaa cggctgcgac atcgaagtgc tcgcctgcct
     4981 tgcgccgcgc tacgacatcg agcagtacgg catctacgtc cacaacaacc cccgtgaggc
     5041 cgacgtcctc ctggtcatcg gctccgtgac gccgcagtgg gtggacaaac tccgcgatat
     5101 atgggataag attccagaac cgaaggtggc cgtcgccatc gggaactgcc cgatatcggg
     5161 gtgcgtctac gcccggagag gcaccctgac caccccgccg gtcgagaact acatccccat
     5221 cgcggcaagc gtgccgggct gtccgccccg cccgacggag atcctctccg ccatactctc
     5281 ggtcgcaccg ctgatattca aagactacga ggagaaacag ccatgaagaa gaccgtcgac
     5341 gtatccatac cgctcggccc ggtccacccc tgctggaaag agcccgtccg cctcaagtgc
     5401 gagacggcgg gcgagcgggt gctcagggtc gaggtcgagc tcggttacat gaagaaaggg
     5461 atcgagcgga tcatgcgggg ccggccctgg caggaggtga tgttccttgc ggaacgtgtc
     5521 tgcggcatct gctcggtcat ccacaacatg gtcttcatcg agacgatgga agagatcagc
     5581 gatatcgccg tcccgccgcg ggcggcctac ctccgggtca tcgtgaacga actcgaccgg
     5641 atagcaagcc acatactcgc aaacttctcc tactgctaca cgatcgagca cgagacgctt
     5701 gccatgtacc tcctgaacac ccgtgagatc gtgctcgaca acctcgagcg gatcacgggc
     5761 tcacgggtca acaccgccta catgataccg ggcggcgtcc ggttcgacct cctgccggag
     5821 gatgcggtga cggtgcttgc cgatctcgat aaggtggaag aggagatgag gcggcacacc
     5881 cggatattcg agaccgggcc cctgatcgcc ctccggagca aggggatcgg ggtcatggat
     5941 cgcgaggagg cgatccggtc ccataccgtc ggccccacgg cccgggcaag cggtgttccc
     6001 gactccgatc tccgcctccg ccacccgacc taccaggcgc tcggctttga gcagataacc
     6061 cggacagagg gcgacaactt cgcccggatc atggtccggt tccaggagat cttccagagc
     6121 atcgacctca tacgaaagtg cgtcactacc ctccccgacg gccccatccg cggaggcggc
     6181 ctctgcaggg ccggggaggg ctcgcacagc ggcgaggcac cccggggcga actgacctac
     6241 acgatcaaga ccgacgagta cgggagggtg ctcgatatcg cgatacgaac tccttcgatc
     6301 atgaacatag aggcctgcgc ccacgcgatg atcgccgacg ccgcatcgat cgccgacgtg
     6361 acgtcgacgt tcatcagcag cgacccctgc gtcgcgtgca acgagaggtg aggtatgcgg
     6421 tcgatcatct actacgtgcg ggagttcctc cggcccgaat ggctcaagaa gttcttcttt
     6481 gcaaagaccg cgcccctcgc caccccatcc tacttcaagg actacccctc cctgacggag
     6541 aaggagtgta cggggtgctt tgcctgtatg atgatctgtc cggcgccggg cggcatcgtg
     6601 gtcctcgcga agaaggacga atgggtgccc acggtctacc cgggccactg catccggtgc
     6661 ggcctctgcg tcgaggcgtg ccccgaaggc gttcttacga gcgggcggat actcgacgtc
     6721 acccgtcgcg ataagacggc gttctcctcc tggtaccgcc tcgaggtcga ggatgccctc
     6781 tgcatgcggt gcgggaactg ctgcgtctcc tgcccggtca accggcaggt agacccccag
     6841 cttgcaggga ccggcacatc gtcaagcgac gaggtgatca tgcggatcga gggcggcagg
     6901 gtgcggatcc tccacgagga gaagtgcacg gggtgcaaga cctgcgagat caactgcccg
     6961 aacagcgcga tccgggttgc ccggatggtg gaaggggtgc agggagagga ggcggaggcg
     7021 tgaacttcct catgatcacc gggcggacgg tgaaccaggg cgtgaccgtc gagaacaaga
     7081 catccgccgc ctatgcagcg gagacatcga cctgtttcat gcacgagttc gatatgctcg
     7141 aactcggtct tgccgatggc gaccccatcc gggtgaccgg gccctgcgga gaggtcgtca
     7201 tgcgggcaat cgggtcgttg gaggtcgaga tgggaagcat ctttgtcccc tacgggcctt
     7261 acgcaaacca catcatcgcc gccggcaccc gctcgaccgg tatgccggac ttcaagtcgc
     7321 acgaagtgac aatcgagccg accgatgaag aaccaaagac cgttcacaga ctgatggaag
     7381 acctgggagg ccttgcctat gatcgttaaa gatgtggtct gcccgttctg cggatgcctc
     7441 tgcgacgaca tcgaggtgga ggtggaggat aaccgggtgg tcgccgtcac gaacgcctgc
     7501 gagctcggca tgacgaagct catgggcgag gagaggatga agaacccgat actgcgcgat
     7561 gggaacacct ggaaggacat cacctacgac gaggcgatcc ggtatgccgc cgatatgctt
     7621 ctatccgccg agcgcccgct cctttatggg tggagcggca ctcacgggga ggcccagtgc
     7681 gtgggggtgc atatggccga actcgtgcac ggcgtcatcg acaacacctc ctcggtctgc
     7741 cacgggccgt ccatcctcgc catccaggag gtcgggcacc cgggctgcac actcggcgtg
     7801 gtgaagaacc gggcggacct cgtgatctac tggggctcaa acccgatcga cgcccacccc
     7861 cgccacctct cccggtacac gcgctatgcg gaagggttct tccttgagaa cgccttccgc
     7921 gaccggaagg tgatcgtcgt cgatatccgg aagaccgaga ccgcgaacgt cgccgacgag
     7981 ttcatgcaga tcaaacccgg cggggactac gcggtcctct ctgcgctccg ggccatcgtc
     8041 cgggggaaga cggagacgat cccgcggacg gtcgcgggcg tcacccgcga gcagctcttc
     8101 cgggtggtga acctctgcaa gaatgcgaag ttcggtgcga tctacttcgg gctcggtctc
     8161 acgatgaccc cggggaagta caagaacatc cggaatgcca tcgaacttac atcggagttg
     8221 agccggcaca caaagttcac catatcggcg atgcggggcc attacaacgt ctatgggtcc
     8281 aacgaagtga acacctggat gaccggctac cccttcggta tcgactactc gcgggggatc
     8341 tccttttaca acccgggcga gacgacggcg gtggatattc tcgccaggaa ggagtgcgat
     8401 gcggcgctga tcgtcgggag cgatccggcc gcccacttcc cgcgccggtg cctggagcat
     8461 ctcgcggata taccggtggt ccagctcgat ccctacccga acgccaccac cgcgttctgc
     8521 aacgtccaga taccggtggc ggtgaccggt atcgacgcgg aagggacggc ctaccgtatg
     8581 gacggtgtgc ccatacggac gaggagggtc ctctccaccg gctacccctc ggatgaagag
     8641 gtcctcaccc gcatgtatgc gctgatgcag ggggcaaggt catgagcgaa ctcctgatcc
     8701 ggaacgcctg cgtcatcgac ccgctccgcg gcatcaacgc cgagacgatg gatatcgcca
     8761 tccgcgacgg ccggatcgtc gaggaggtga gcgctgcggc ggaggtgctc gacgcccgcg
     8821 gcatgctcac cctcccgggc gggatcgatt cgcacaccca catatgcggg acgaaggtga
     8881 acttcgggcg ctacatgagc cccgaggata tgcgggcggg gaggacgccc cggcgcggcc
     8941 cgatgcacgc cacgtccggc tacagcgtcc cgaccaccta cggcaacagt taccgctaca
     9001 gtgcgatggg ctacaccacc ctgcttgagg gcgcgatggc gccgcttgag gcccggcaca
     9061 ctcacgagga gttcacctac acgccgctgc aggacacgat ggcaaacacc ctctttgacg
     9121 ggaactgggg gatcatggag gcgatccacg acggcgacac gaaacgggtt gccgccatca
     9181 tagcctggac gctcacggcg gcaaaggggt tcggtgtcaa actcacgaac cccggcggca
     9241 cggaggcctg gcagtggggc aagaacgtct cgtgcattcg tgacccggtg ccctacttcg
     9301 aggtgacgcc ggccgagatc atccggtcga tcatcgaggc gaacgagctc ctccgccttc
     9361 cgcactcggt ccacctccac tgtaacaacc tcgggacgcc ggggaactac acctgcaccc
     9421 tcgggtcgct gggcctcgtg ccggacctga acgagaagcg gcagacgctg tacgcaaccc
     9481 acgtccagtt ccacgcttac ggtgggtcgg ggtggaagga tttctgttcg aagagcgagc
     9541 cgatcgcgcg gttcgtcaac atgcgcccac agatcgtcat cgatatgggg caggtgatgt
     9601 tcggccggac gacgacgatg accgccgacg ggccgatgga gttcaacctc taccgtctcc
     9661 accacgacaa gtggagcaac cacgacgtgg agctcgagac cgggtccggc atcatcccgg
     9721 ttctctaccg gcgcaagaac ctggtcaact cgatcatgtg ggcgatcggc ctcgagctcg
     9781 cgctcctcac gaagagtccc tggcagtgca tgctcacgac ggataacccg aacggcgcgc
     9841 cgttcgtccg ctaccccgag atcatcgcgc tcctcatgag caagaggtac cgggacgcag
     9901 agttcgcgag gatccatccc gacaccggcg gacgggtgcc cctcccggcg atcgagcggg
     9961 aactggactg gcacgagatc gcggtgatga cccgggccgg ccaggcaagg gcgctcggca
    10021 tccaggacct cgggaaaggc tacctcgggc cgggtgcgga agcggacgtc gccatctacc
    10081 cgatccggat cgacgagacc gatccgtcgg tcgactacca gaaggtgatc gaggcgttca
    10141 gccggaccga gtacacgatc aagcggggca gggtcgtctc ccggcggggc gagtgcctgg
    10201 tcgacgggag caacgcgacg ttctgggtga gggcgaaggt ctcggatgcc tacgatatgg
    10261 gtaaagaccc cgactttata gagaagttcg accgctacta cacggtccgg atgaggaact
    10321 acccggtgca ggaggcatac ctgaaccgga accggtgcat cgagacggag gcggcgatat
    10381 gagggtcacg ctcgcgatga agcccgctga gcggtcgttc atccccatcg aggcggaggc
    10441 gatcgtcccg aagaacttcc ttgccggcac cgatatatcg gtctggcggg ggaaccgcga
    10501 actcaggctc gacgaggtct tctcggtctc tgtggagggc gacgccgacc ggccggagga
    10561 tgtcgaggtc gtcctctcgg gagacgagac attccggttg aagagggtcg gggagtacat
    10621 ggacggcggg aagatcgtcg tcctcggcgg tatcggtatg cactgcggca acttcatgag
    10681 cggcggcacg atcgagatcc acgggaacgc cgacggctgg ctcgggcggg agatggcggg
    10741 ggggaccatc ctctgccggg gcgacgccgc cgattactgc gcgtcggggt atcgtggagg
    10801 gcggaaaggc atgaccggcg ggaccgtgga ggtcttcggc cgcgccggcg atttcctcgc
    10861 ggagagcctc gccggcggca ccgtgatcgt gcacggcggc gccggggata tggccggggc
    10921 ggagatgcac ggcggcacgc tcatcgttca cggggactgc agccgcccgg cggcgaacat
    10981 gaaggccggc tcctgctacg tctacgggac cgcgcacggg atgctcccga cgtttaagag
    11041 gatcggggcg gtccagcatg atggacgcac acttatccag tttacgggcg atattgcgaa
    11101 tcgggggaaa ggaaaccttt ttgtaaagga ctatcgatat ctggattgat ggtcaggcac
    11161 gcgtgcgggt tcgatgcccc gattcactgc aagaggtgcg ggcgaccgct ggagagcaac
    11221 gaacgtaagg gcctcttctg cccgcactgc ggaagaaggg tgagcatcgt ctgcccgggt
    11281 tgcgggcgcc tctggtgatc agacccggtt cttcacccac tcggtgtatt gggttctcaa
    11341 ctgccggata tcctgttcgg agagatcctt ctttcctgtc cggtgcaggt aggactcaag
    11401 ctgctccacc acccgttctg catctaaaaa gtcgtcgaca tccaggtaga ccggcgcacg
    11461 cccgacgttg atcgcctcgc cgcacatcgg gcagagcctc cagcgctggt agcggtcgac
    11521 gtaggtgaag gtcctgcagc ccgggcaacg gatgataagg tacatgatca atgggagtgt
    11581 gcgagcgtgc ggatatataa ttatccatac cgctctctct tccggccggc ctcaccgtag
    11641 gggatacccg cgcgccactg tccggcaacc ctcaccccgg ccttcgtggg ggatacccat
//
LOCUS       DAOSII010000059        11655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_5504_length_11655_cov_43.765948, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000059 DAOSII010000000
VERSION     DAOSII010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11655
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5504_length_11655_cov_43.765948"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PLV90_05880"
     CDS             <1..393
                     /locus_tag="PLV90_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOF96681.1"
                     /translation="GRIARAIVEDLQEGFRSGQCTVSIDGALIVCNTRFSEHAKRYAA
                     CRGIEHIGWDYPEGQNLKTMIEETQSYPVTIVSGMSSSVSARLASAGILTAKQVAYGD
                     ATTIARDTALSLPEVLVIAGRARAILER"
     gene            complement(401..1279)
                     /locus_tag="PLV90_05885"
     CDS             complement(401..1279)
                     /locus_tag="PLV90_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOF96682.1"
                     /translation="MLIMLPSMTGYMAWEAQIVDITTLEIDGAPEGVVYITDPHVGPS
                     SIDHIQKVIQEVNRLNPSLVLIGGDFATGDEENFTYQGVWGSLDAPAYAVLGNHDYRV
                     GTDGATGLERTIAVWTSTNTTTAGYDVSALNDGSADTAFADGLAATLEENGVYVLRND
                     YARVDAGGEEIVIVGVDDGWAGMADPPDVPATDAFTIYLIHEPSCRADWDADLILAGH
                     THGGQFLFPVVAQLNDIGVIELAGLFETNGTPTYISRGVSSAGIAGVGLRFNCRPEIV
                     LINPTEDQLRVLGNPA"
     gene            complement(1380..2036)
                     /locus_tag="PLV90_05890"
     CDS             complement(1380..2036)
                     /locus_tag="PLV90_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96683.1"
                     /translation="MTCLDSSRGVYLTYFIQGLILLAAAASIMGGEYLSGLSAAFAFL
                     LTLAPSFATRNMRFCLPWGVNLLIAVSLYLHVMGYTGGYYAALAYYDKLAHLVSSATV
                     ALIVFSLMVLAGHRGDVRLTTPAVVASILIATLAAGALWEIYEFAVDQIFGTSLQLGN
                     TDTMWDLIMDLIGAAIVAGFAAFVLAREDRFTGIFAGLISVAEPADVMDNQADSAKSR
                     "
     gene            2271..2597
                     /gene="eif1A"
                     /locus_tag="PLV90_05895"
     CDS             2271..2597
                     /gene="eif1A"
                     /locus_tag="PLV90_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor eIF-1A"
                     /protein_id="HOF96684.1"
                     /translation="MTDPSKRKPESNGGDAEGEIIRVRLPNKRNREMFGSAELMLGAN
                     HIRIRCFDGVTRTGRIKGKIKKKVWIREGDVLIVVPWSFQDEKCDIIYRYTRPQVEWL
                     RKNRYL"
     gene            complement(2675..3427)
                     /locus_tag="PLV90_05900"
     CDS             complement(2675..3427)
                     /locus_tag="PLV90_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96685.1"
                     /translation="MINEKAVSEAIGFILILGIVLTGIGLVTLYGYPVLVKEQSNADV
                     RNMERAMIVIQNDMKSLCFKNVPYKETTLQVSGGTLEVIDSGDSGSTFTITGDGIDPD
                     VAFHPGALVYRSDRGTEVITLENGAVMTRQADATGSAMLAEPRWFYDGPTKTFVVYLM
                     NITADERMAKSGMATVRMSLASTASQPYTGSDPIKVTYTRDADNDLSVAWENYLTALG
                     MLKTDPNKYEWDINKPVEKLVIKEYKIRILGI"
     gene            complement(3424..3882)
                     /locus_tag="PLV90_05905"
     CDS             complement(3424..3882)
                     /locus_tag="PLV90_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96686.1"
                     /translation="MRPCIIEPMDEEGVTRLMEYIIITGVLLLLMIVMMFSVNASIME
                     GPANKLRYHAFVDIGNGVSARIVDLYVIAPGNGTIETKFDIPDDVAGKGYFVDVWMEG
                     ENRASQAIEVQGGAIKSKVAIAGIGATRGVIGNTTGAGWNMIQYDSKGFL"
     gene            complement(3863..4237)
                     /locus_tag="PLV90_05910"
     CDS             complement(3863..4237)
                     /locus_tag="PLV90_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96687.1"
                     /translation="MNEEGQWIVLMGLLVAVALFFLALIINQSALVGQTTAEGVLEFP
                     KNDIRDLREAVFDYVDAAGALDPIERVDIEKDIITISLERKNAVVQFGNGTPQDVSGR
                     WLRPVDIHYNNGVTVYNETVYY"
     gene            complement(4234..4755)
                     /locus_tag="PLV90_05915"
     CDS             complement(4234..4755)
                     /locus_tag="PLV90_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96688.1"
                     /translation="MVNDTGQLYTMEGVAAGVIMLLAAYIVVSTTSIYTTGDTHIPDM
                     QLEQLGSDVLAMMDAPDTNGTTSALETFVSTNNGTGFNSTFLGYCNATTRGTLDNLHM
                     SANITYRDANANKINEYPFVAPDNTWTGRESAVRATRWVQLPKLSPKPVGMPGDMRTD
                     PQAVLVEVLLWRA"
     gene            complement(4749..>4951)
                     /locus_tag="PLV90_05920"
     CDS             complement(4749..>4951)
                     /locus_tag="PLV90_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96689.1"
                     /translation="SSISLVLKPAATSLFTLDQNSILDIRFNFEDDPPRTNITGTHLY
                     DYNPENVTHAGLKTAVLEVAIW"
     assembly_gap    4952..5051
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5052..5834)
                     /locus_tag="PLV90_05925"
     CDS             complement(<5052..5834)
                     /locus_tag="PLV90_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96690.1"
                     /translation="MADDGVLSLDFLAGFTIFMLALVMVLSMVPGLLAGLESSGIDYD
                     AVAYRTGVILVEDPGWPAYPPWELYDRDNKEEIQRMGLAVSKETPNILLSTKVEKFFE
                     ESFFTSEDYRSKVIFGDIPYSYNISLRSETYNYMTGDPLPPGYGYIRRVVKIKEPGVA
                     EIKSPGPEYVIPDPSAPEWNSTARNFTVRLNFSQLLDPGRGPAYRIDPRTEPVNITIT
                     NFGEYRNITGPSVNATLHNVTFWKMDPTRPNPRFTRIPFSYDT"
     gene            complement(5840..6403)
                     /locus_tag="PLV90_05930"
     CDS             complement(5840..6403)
                     /locus_tag="PLV90_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96691.1"
                     /translation="MRTSGSVPALIATVCIFCATCGCLGAAPLPTGGGITPIDLPEPP
                     AASTFADALAELDLLGAEGGLNVTGASVRQVLGTGVEIDGQAASWALGLQDGEEIRWL
                     TYGPAGWQEISFSAPLPADEVNVTGVLSPEDLLRGREDMFRPVMERLGADTVDLALAG
                     GVYSVTIRSDAGMETLSFEADTGEVIA"
     gene            6676..7293
                     /locus_tag="PLV90_05935"
     CDS             6676..7293
                     /locus_tag="PLV90_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HOF96692.1"
                     /translation="MPGEAHDRFAEAARFHGHICPGLALGYRAAGIALERLRSGRAED
                     EELVTIAETDACGVDAIQVLTGCTAGKGNLLFKDHGKHAFTFINRVTGDAVRVVGNPS
                     FDTDSLDPGLASLRTRVMQGQASGEELAEYRRRIDRIVEKILDLPAETLFTIRKVDVD
                     MPERARIFKSVPCAKCGELTAESRVRVEDGKFVCRSCSKEYTRRW"
     gene            complement(7303..8175)
                     /gene="hisG"
                     /locus_tag="PLV90_05940"
     CDS             complement(7303..8175)
                     /gene="hisG"
                     /locus_tag="PLV90_05940"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOF96693.1"
                     /translation="MITIALPKGSLEAQTLQLFKEADLEVRRTDRDYNPRINDPRIGK
                     VKILRPQEIPLYVQMGYFDIGISGLDWVQESGADVREIANLSYSKTGDGNVKIVVAVH
                     RDEPIDSIAAIRPDSRVTTEYPRITERFFKENGVPVRLFASYGASEAKVPDLMDVVVD
                     LTETGSTLKKNGLKIVGQIMESHTALLANHEAFADPEKRREIEEITTLLLGVIEARHQ
                     VLLTMNVPSGALDRVIDVLPAMKKPTVGRLHGIDYFSIQTVVQKGLVNGLIPHLKAAG
                     AEDILEIPITKIVR"
     gene            complement(8222..9484)
                     /locus_tag="PLV90_05945"
     CDS             complement(8222..9484)
                     /locus_tag="PLV90_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOF96694.1"
                     /translation="MVQIYRFAAVRPERRYSGDIPSVPYDVVTAEEARECIEKNPLSF
                     LRVSRPDAELPDLPPHDDRVYERAREVFYGLLADGRLQRDPEPGMYVYRAVQDGEEFV
                     GLVCCVATEEYENRRIRRHELTRYDKEEDRTRHIDAVGANTGLVFLLYRDPDDIYTAI
                     RSLIDGAEPDGSTTTVSGVLHQVYRITDEAALSHLEGLFATVPEAYIADGHHRAKSAV
                     NVAERRREEGRFTEEAGRFMVVLFARDHVRIHGYSRLVTDLGGLTLPGFIDALLRAGW
                     SIRPHGKVDASEYQVRPLATGDEPLHVMHFYMQGTWYEVSRPVDNPADLIGSLDVSVL
                     QKEVLEGMLGINDPRGDPRLHYMGGAKPLTGLERLVDSGEYALAVAMQPVDVETVLAI
                     ADADGVMPPKSTWFEPKLLSGLVVHTID"
     gene            9577..>10023
                     /gene="rimI"
                     /locus_tag="PLV90_05950"
     CDS             9577..>10023
                     /gene="rimI"
                     /locus_tag="PLV90_05950"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOF96695.1"
                     /translation="MPLQLTIRRAQPADIPQIVAIERMAFTDPWDERTLCESLAYFPE
                     TFFVARNNGDMAGFVAAGLEDTGEDIYGHIMNLAVAPGYRRQGVGQHLICRLEQEFML
                     SGANAAQLEVRISNTGAQGFYHRLGYREVFRIASYYANGEDALIMMK"
     assembly_gap    10024..10123
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10124..11085)
                     /locus_tag="PLV90_05955"
     CDS             complement(<10124..11085)
                     /locus_tag="PLV90_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HOF96696.1"
                     /translation="MDEYIERLKDGSLKLYALEKELPPAEAVRVRRAFIEGETHTALT
                     GVGSFSIDIDRVVKANIENMIGTVQVPLGVAGPLRVKGEYADGSYYLPLATTEGALVA
                     SVNRGCSLITRAGGADVRIMRDGMTRAPVFAARDVGHAHGVAAWVDEHIEDIRRAAES
                     TTKHGKFLDAATYVAGTSVYVRLEFDTKDAMGMNMVTIASQKVGELIERETGARLIAL
                     SGNMCTDKKPAAINLVRGRGKTVVAGVRLTDAMIADLLKTDADTLAEANYRKNLVGSA
                     RAASLGFNAHAANVVAAMFIACGQDPAHVVEGSSAITTVDRVDGG"
     gene            11120..>11655
                     /locus_tag="PLV90_05960"
     CDS             11120..>11655
                     /locus_tag="PLV90_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOF96697.1"
                     /translation="MVDVDAEQWCLVVEKREAEQTRRRLLEEGLLDRRYKPRSEGDAV
                     LLPVTEEIAGATRRTFVTVPQRADLPRHDLVGGIAILQEDDPEAAKRLLASRPSLHTV
                     LFPETPVEGEYRTRRFAVLAGTPTTRTRVTEYGLRFDVDLAHAYFSARLSTERQRILG
                     MMEDGERVLDMFAGVGPFA"
BASE COUNT         2120 a         3529 c         3609 g         2197 t
ORIGIN      
        1 ggacggatcg cccgggcgat cgtcgaggac ctccaggagg ggttccggtc cgggcagtgc
       61 accgtctcga tcgatggcgc cctgatcgtc tgcaacacca ggttctcgga gcacgcaaag
      121 cgctacgcgg cctgtcgggg gatcgagcat atcgggtggg attatccgga ggggcagaac
      181 ctgaagacga tgatcgagga gacgcagtct tacccggtca ccatcgtcag cgggatgagt
      241 tcatcggtct ccgccaggct cgcgtccgcc gggatactca cggcaaaaca ggtcgcttac
      301 ggcgacgcga caaccatcgc ccgggacacg gccctctccc tcccggaagt gctggtgatt
      361 gccggccggg cgcgcgcgat cctcgaacgg tgaggggatc tcaggcgggg ttgccgagga
      421 cccggagctg gtcttccgtc gggttgatca ggacgatctc gggccggcag ttgaaccgga
      481 ggcccacgcc ggcgatgcct gcgctcgaga ccccgcggga tatgtaggtc ggcgtcccgt
      541 tcgtctcaaa gaggccggcg agctcgatga cgccgatgtc gtttaactgt gcgacgaccg
      601 ggaagaggaa ctggccgccg tgggtgtggc cggcgagtat gaggtctgca tcccagtcgg
      661 ccctgcagga cggttcgtgg atgagataga tcgtgaatgc gtcggtcgcc ggcacgtcgg
      721 gcgggtctgc catcccggcc cacccgtcgt cgacgccgac gatgacgatc tcttctcccc
      781 cggcatcgac ccgggcgtag tcgttccgga ggacgtatac cccgttctcc tccagcgtcg
      841 cggcgagccc gtcggcaaat gcggtgtcgg cagatccgtc gttcagggcg gagacgtcgt
      901 acccggcggt ggtggtgttg gtggaggtcc agacggcaat tgtcctctca aggcccgtcg
      961 caccgtcggt ccccacccgg tagtcgtggt tcccgagaac ggcgtaggcc ggtgcatcaa
     1021 gcgatcccca taccccctgg taggtgaagt tctcctcgtc gccggtggca aagtcaccgc
     1081 cgatcaggac gagcgagggg ttcaagcggt tgacctcctg gatcaccttc tggatgtggt
     1141 cgatgctgga cggcccgacg tgggggtcgg tgatatagac gacaccctcc ggtgccccgt
     1201 cgatctcaag ggtggtaata tcgacgatct gcgcttccca ggccatgtaa ccggtcatgg
     1261 acgggagcat gatgagcatg aacaacgcca gatgtctggc acttggggtt tttaaggtca
     1321 ctctatttta tttcaccttc caaatattaa tagagtccga cgatccgggc gagagcggat
     1381 cagcggcttt tcgccgaatc cgcctggtta tccatgacat cggccggctc tgcaaccgag
     1441 atcagtccgg cgaagatccc ggtaaatcgg tcttccctcg caagaacgaa agcggcgaac
     1501 cccgccacga tcgccgcccc gatgagatcc atgatgaggt cccacatggt atcggtgttc
     1561 ccaagctgga ggctcgtgcc gaatatctgg tcgactgcaa actcgtagat ctcccacagc
     1621 gctcctgctg ccagcgtggc aatcaggatg gaggcgacga ccgccggcgt cgtcaggcgg
     1681 acgtctccgc ggtgcccggc aaggaccatg agagagaaga cgatgagcgc gacggtggcc
     1741 gatgagacga ggtgagccag tttgtcatag taagcgaggg cggcatagta gcccccggta
     1801 taccccataa cgtggagata gagggagacg gcgatgagga ggttgacccc ccagggcagg
     1861 cagaaacgca tatttctcgt cgcgaaggac ggcgccagcg tcaggaggaa ggcgaaagct
     1921 gccgagagcc cggagaggta ctcgcctccc atgatgcttg cggctgcggc gaggaggatg
     1981 agcccctgga tgaagtaggt gaggtacacc ccgcgcgacg agtccaggca tgtcatgggt
     2041 acattgaata ggggatgatc ccggcaatat acataaccac ccgggcgatg gtctccggga
     2101 agagggcggt tatcccggga ccggatttgg gggacggcac ggtcgaccgg ggggggatcg
     2161 ccggccgttg cagccgcctc cgcgtgtccc gggagaatgc ttgataagct agcagttgta
     2221 taaataggac acacgaagga ccagatgctc cggaacatgg ggttgatgaa ctgaccgatc
     2281 ctagcaagcg caaacctgaa tccaatggag gagacgcgga aggggagatc atccgcgtgc
     2341 gcctgccgaa caagaggaac cgggagatgt tcggcagcgc tgaactgatg ctcggggcaa
     2401 accatatcag aatccgctgt ttcgatgggg tgacgcgcac cgggcggatc aagggcaaga
     2461 tcaagaagaa ggtctggata cgggaaggag atgtcctgat cgtcgttccc tggagtttcc
     2521 aggatgagaa gtgcgacatc atctaccgct acacgagacc tcaggtggaa tggctccgga
     2581 agaaccggta tctctgaatc ctttttgagc aggaattttc tgcacggccg gcaggagggc
     2641 gtccccctcc tgccggcgac gggttccagc ggggttagat gccgaggatc ctgatcttgt
     2701 actcttttat gacgagtttt tcgacgggtt tgttgatatc ccactcatac ttgttggggt
     2761 cggttttgag catcccgagc gcggtcaggt agttctccca cgccacggaa aggtcgttgt
     2821 cggcatccct ggtgtatgtt accttgatgg ggtcggagcc tgtatacggt tgcgaagccg
     2881 tgctcgcgag gctcatcctc accgtcgcca tccccgactt cgccatccgt tcgtcggcgg
     2941 ttatgttcat gaggtagacg acgaacgtct tcgtggggcc gtcatagaac caccgcggct
     3001 cggcaagcat cgccgaaccc gttgcatccg cctggcgcgt catcaccgcc ccgttctcaa
     3061 gggtgatcac ctcggtgccg cggtcggatc ggtagaccag ggcaccggga tggaacgcaa
     3121 catcaggatc gataccgtca ccggtgatcg tgaatgtcga accggaatcg ccggagtcga
     3181 tcacctcgag cgtccccccg ctcacctgga gcgtcgtctc cttgtagggc acgttcttga
     3241 agcagaggct cttcatgtcg ttctggatga cgatcattgc ccgctccatg ttcctgacgt
     3301 cggcgttgct ctgctctttg acgagcaccg ggtagccgta gagcgtaacg aggccgatac
     3361 cggtgagcac gatccccagg atcaggataa atccgatcgc ctccgaaact gccttctcat
     3421 ttatcataaa aaccccttcg agtcgtactg gatcatgttc caccccgcac cggtcgtgtt
     3481 gccgatgaca cccctcgttg ccccgatgcc ggcaatggcg accttgctct tgatggcgcc
     3541 gccctgcacc tctatcgcct ggcttgcccg gttctcgccc tccatccaca cgtccacgaa
     3601 gtagcctttc ccggcgacgt cgtcggggat gtcgaacttc gtctcgatgg tcccgttgcc
     3661 gggcgcgatc acgtagaggt ccacgatccg ggcgctgaca ccgttcccga tgtccacgaa
     3721 ggcgtggtag cggagtttgt tcgccggccc ctccatgatc gaggcgttga cggagaacat
     3781 catgacgatc atcaagagga ggagcacgcc ggtgatgatg atatactcca taagccgggt
     3841 gacgccctcc tcatccatcg gttcaataat acacggtctc attgtacacc gtcaccccgt
     3901 tgttgtagtg gatgtctacc ggccggagcc agcgcccgga gacgtcctgc ggcgttccgt
     3961 tcccgaactg taccacggcg ttcttccgtt cgagggatat tgtgatgata tccttctcaa
     4021 tatccacacg ttctataggg tctaacgccc cagcagcatc gacgtagtca aacaccgcct
     4081 ctcgtagatc ccggatatcg ttcttcggga actcaagcac cccctccgcg gtcgtctgcc
     4141 cgacgagcgc cgactggttg atgatcagcg cgaggaagaa gagcgccacg gcgaccagga
     4201 gccccatcag gacgatccac tggccttctt cgttcatgcg cgccacaaaa gcacctccac
     4261 gaggacggcc tgagggtctg ttcgcatatc accaggcatg ccgaccggct ttggggataa
     4321 ttttggcagt tgcacccacc gggtcgcccg cacggcggat tctcttcccg tccaggtgtt
     4381 gtcgggtgca acgaacgggt actcattaat cttgttcgcg tttgcatccc ggtaggtgat
     4441 gtttgcactc atgtgcaggt tatcgagcgt accccttgtc gttgcgttgc agtagcccag
     4501 gaacgtgctg ttgaaaccgg taccgttgtt cgtgctgaca aaagtctcca gggcgctcgt
     4561 tgtgccattg gtatccggcg cgtccatcat agcgagcacg tcgctcccga gctgctctag
     4621 ctgcatatcc gggatgtgcg tatctcccgt agtgtagatg ctcgtcgtgc tgacgacgat
     4681 gtacgcggcg agcagcatga tgaccccggc cgcgacaccc tccatcgtgt agagctgtcc
     4741 tgtatcgttc accatatggc gacctccagg acggcggttt tgaggccggc gtgggtgacg
     4801 ttttctggat tgtagtcata caggtgcgtg ccggtgatgt tggtccgcgg ggggtcgtcc
     4861 tcgaagttaa accggatatc gaggatgctg ttctggtcga gggtgaagag tgaggtggcc
     4921 gccggtttca ggacgaggga gatgctggaa tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn ncgtatcgta cgagaacggg atgcgggtga accgcgggtt tggccgtgtc
     5101 ggatccatct tccagaacgt gacgttgtgg agcgttgcgt tcacggatgg tccggtaatg
     5161 ttacgatact ctccgaagtt cgtgatcgtg atgttcaccg gttcggtcct cgggtctatc
     5221 cggtaggccg ggccccgccc cgggtcaagg agctggctga agttgagccg gacggtaaag
     5281 tttctcgcgg tggagttcca ctccggagca gatggatccg gtatgacgta ctccggcccg
     5341 gggctcttga tctccgccac ccccggctcc ttgatcttca ccacccggcg gatgtagccg
     5401 tagccgggag ggagggggtc gccggtcatg tagttgtatg tctcgctccg gagcgagata
     5461 ttgtaagaat aggggatgtc gccgaagatc accttgctgc ggtaatcctc ggaagtgaag
     5521 aacgattcct caaaaaattt ctcgaccttc gtcgagagga ggatattcgg cgtctctttc
     5581 gagacggcaa gccccatccg ctgaatctcc tccttgttat cccggtcgta gagctcccac
     5641 ggcgggtagg ccggccaccc cggatcctcg acgaggatca cccctgtccg gtaggcgacc
     5701 gcgtcgtagt cgatgccgct gctctcaagg ccggcaagga gccccggcac catgctcagc
     5761 accatgacca gggcgagcat gaagatggtg aaccccgcga gaaagtcaag cgagagcacc
     5821 ccgtcgtcgg ccaccgatct tatgcgatca cctccccggt atccgcctca aacgagaggg
     5881 tctccatccc ggcatccgag cggatggtga cggaatacac ccccccggcg agagcaagat
     5941 cgaccgtatc ggcaccgagc cgctccatca cgggccggaa catatcctcc cggccgcgaa
     6001 ggaggtcttc gggagagagg acgccggtga cgttcacctc atccgcgggc agaggggccg
     6061 agaacgatat ctcctgccag cccgcagggc cataggtcag ccaccggatc tcctcgccat
     6121 cctggaggcc gagggcccag gaagcggctt gaccgtcgat ctcgacgccg gtcccgagca
     6181 cctggcgcac cgatgccccg gtgacgttca ggccgccctc ggcaccgagc aggtccagct
     6241 ccgcgagagc atcggcgaag gtcgaggccg ccggcggttc cggcaggtcg atcggggtga
     6301 tgccgccgcc ggtcggtagc ggggctgcac caagacaacc gcacgtggca cagaagatac
     6361 agaccgtggc aatcagggcc gggacgcttc cggaagttct catgcaaggc actcattata
     6421 cacgaagggg tataaataat catcccccgc ggggagtcaa gaatccgtca ccatccctcg
     6481 ataccggaaa cgtgcaggta cccggcggca aaaatatttc gtctcaaatg agggccggaa
     6541 atccggagca cccgggtctt tatatgccag gagcccctgc ccgcaggccc cggcagggca
     6601 tcgtaactta tatctctctg ttagtagcaa catgtcatgc aaggtaagca catgtgtact
     6661 actacacccc ccggcatgcc gggagaggca cacgaccggt tcgccgaagc agcccggttc
     6721 cacggccaca tctgccccgg ccttgctctg gggtatcggg cggccggtat cgctcttgaa
     6781 cggctccgct ccggccgggc cgaggacgaa gaactcgtca ccatcgccga gacagatgcc
     6841 tgcggcgtcg atgccatcca ggtcctgacg gggtgcaccg ccggaaaagg aaacctcctc
     6901 tttaaggacc acggcaaaca cgccttcacg ttcatcaacc gcgtgaccgg cgatgcggtt
     6961 cgggtcgtcg ggaacccgtc gttcgatacc gactccctcg accccggcct tgcatcgctc
     7021 aggacccggg tcatgcaggg ccaggcctcc ggtgaggaac tggccgagta ccggaggcgg
     7081 atagaccgga tcgtcgagaa gatcctcgac ctgcccgccg agaccctctt caccatccga
     7141 aaggtcgacg tcgatatgcc ggagcgggcc cggatattta agtcggtgcc gtgcgcaaaa
     7201 tgcggcgaat tgaccgccga gtcacgggtg cgggtcgagg acggcaaatt cgtctgccgt
     7261 tcctgctcaa aagaatacac ccggcgctgg tgaggggacc cctcaccgca cgatcttcgt
     7321 tatcggtatc tcaagtatat cctcggcacc cgcagccttc aggtgcggga tgagaccgtt
     7381 gacaagccct ttctgcacca ccgtctgtat gctgaagtag tcgatcccgt gcaaccggcc
     7441 gacggtgggc ttcttcatcg cggggaggac atcgatgacg cggtcaagcg cccccgaggg
     7501 cacgttcatc gtcaggagaa cctggtggcg cgcctcgatc acgccgagga ggagggtcgt
     7561 gatctcctcg atctcccggc gcttctccgg gtcggcgaaa gcctcgtggt ttgcaagcag
     7621 cgcggtgtgc gactccatga tctgcccgac gatcttcaac ccgttcttct tgagcgtact
     7681 cccggtctcc gtgaggtcga cgacgacgtc catcaggtcg ggcaccttcg cctccgatgc
     7741 cccgtaagag gcaaaaagcc tgacggggac gccgttctcc ttgaagaacc tctccgtgat
     7801 ccggggatac tcggtcgtca cccggctgtc cggccggatg gcggcgatgc tgtcgatcgg
     7861 ctcgtcgcgg tggaccgcga ccacgatctt cacgtttccg tcgcccgtct tgctgtagga
     7921 gaggtttgcg atctccctga cgtcggctcc gctctcctgc acccagtcaa gccccgatat
     7981 accgatatcg aagtagccca tctggacgta gagcgggatc tcctgcgggc ggaggatctt
     8041 cactttcccg atccggggat cgttgatgcg ggggttgtag tcgcggtcgg tccgcctgac
     8101 ctcaaggtcc gcctccttga agagctggag cgtctgcgcc tcgaggctcc ccttcgggag
     8161 ggcaatcgtt atcataagag ttgatcggtc agaaaaggga ttaaaggtga tgatccggat
     8221 ctcagtcgat ggtgtggacg acgaggcccg agaggagttt cggctcaaac caggtggact
     8281 tcgggggcat gacaccgtcg gcatcggcga tagcaagcac cgtctcgaca tctaccggct
     8341 gcatcgccac cgcaagcgca tattcgccgg aatccaccag acgttcaagc ccggtgaggg
     8401 gttttgcccc gcccatgtag tggagcctcg ggtctccccg gggatcgttg atgccgagca
     8461 tcccttcgag gacctccttc tggagcaccg agacgtcgag cgatcctatc aggtcggcgg
     8521 ggttatcgac cggccgggag acctcgtacc aggtcccctg catgtagaag tgcatgacgt
     8581 ggagcggttc atccccggtc gcgagcggcc ggacctggta ctccgaggcg tcgaccttcc
     8641 cgtgcgggcg gatgctccag cctgcccgca ggagcgcatc gatgaacccg gggagggtga
     8701 gaccgccgag atcggtgacg agccggctgt agccgtggat gcgaacgtgg tcccgggcaa
     8761 agaggacgac catgaacctc cccgcctcct cggtgaacct gccctcctcc cggcgccgct
     8821 cggccacgtt caccgccgat ttggcccggt ggtgcccgtc tgctatgtag gcctccggca
     8881 cggttgcaaa gagcccttca aggtgggaga gggccgcctc atcggtgatc cggtagacct
     8941 ggtggagcac ccccgacacg gtcgtggtgc tcccgtccgg ctccgccccg tcgatgaggg
     9001 agcggatggc ggtgtagata tcgtccgggt cacgataaag gaggaagacg aggccggtgt
     9061 ttgcccccac cgcatcgatg tgccgggtcc ggtcctcttc cttgtcgtac cgggtgagtt
     9121 cgtgccgccg gatccgccgg ttctcgtact cctccgtcgc cacgcagcag accagcccga
     9181 cgaactcctc gccgtcctgg acggcccggt agacgtacat gcccggctcc gggtccctct
     9241 gcagccggcc atcggcgagc aggccgtaaa agacctcccg tgcccgctcg tagacccggt
     9301 cgtcgtgcgg agggagatcg gggagctctg cgtccggccg gctgacccgc aggaaactca
     9361 gggggttctt ctcgatacac tcccgggcct cctctgccgt caccacatcg tacggcacgg
     9421 aaggtatatc cccggagtac cgccgctcag gacggaccgc tgcaaaacga tagatctgga
     9481 ccatgaaaac cgccacactc gtgagtgcct actattttgt ctcctgacac tatattaggt
     9541 agggcactcc aggaaggggg gtgaccggta acgatgatgc cgctacaact caccattcgc
     9601 agggcgcagc ctgcagatat tccacagata gttgccatcg agaggatggc gttcaccgac
     9661 ccctgggatg agagaacgct ctgcgaatcg ctcgcctact tcccggagac gttctttgtg
     9721 gcacggaaca acggggatat ggccgggttc gtcgccgcgg gccttgaaga cacgggtgaa
     9781 gacatatacg ggcacatcat gaaccttgcg gtcgcaccgg ggtaccggcg ccagggagtc
     9841 gggcagcacc ttatctgccg cctggaacag gagttcatgc tctcgggtgc gaacgccgcc
     9901 cagcttgagg tccggatcag caacaccggg gcgcaggggt tctaccaccg cctcggctac
     9961 cgggaggtct tccggatcgc atcgtattac gcgaacggtg aagatgccct tatcatgatg
    10021 aagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccgccgt ccacccggtc
    10141 gacggtggtg atcgcgctgc tcccctcgac gacgtgggcc gggtcctgcc cgcaggcgat
    10201 gaacatggcg gcaacgacgt ttgccgcatg ggcgttgaac ccgagcgagg ccgcccgcgc
    10261 agagccgacc aggttctttc tgtagttggc ttcagcgagc gtatccgcgt cggtcttcaa
    10321 gaggtcggcg atcatcgcgt cggtcaggcg cacgccggca accacggtct tcccccggcc
    10381 ccggaccagg ttgatcgccg ccggcttttt gtcggtgcac atgttccccg agagggcgat
    10441 gaggcgggcg ccggtctccc gctcgatgag ttcgccgacc ttctggcttg cgatcgtcac
    10501 catgttcatg cccatggcgt ctttggtatc gaactcgagc cggacgtaga cgctcgtgcc
    10561 ggcaacgtag gtggcggcgt cgaggaactt cccgtgtttc gtggtgctct ccgcagctct
    10621 ccggatgtcc tcgatatgct cgtccaccca ggccgccaca ccgtgggcgt ggccgacgtc
    10681 gcgggcggca aagaccggcg cacgggtcat gccgtcccgc atgatccgga catccgcgcc
    10741 tcccgcccgg gtgatgagag agcacccgcg gttcaccgat gcgacgagcg ccccctccgt
    10801 cgtcgcgagg ggaaggtagt aggacccgtc ggcgtactcg cctttcaccc ggagcgggcc
    10861 cgcaacgccg agcggcacct gcaccgtgcc gatcatgttc tcgatgttcg ccttcaccac
    10921 ccggtcgata tcgatggaaa acgacccgac cccggtaagg gcggtgtgag tctcgccctc
    10981 gatgaacgcc cgccgcaccc tgaccgcctc ggcgggcggg agttccttct caagcgcgta
    11041 gagcttgaga gagccgtctt tgagcctttc gatatactca tccatgaata gtatgagaac
    11101 gcatacctaa agatggctgg tggtcgatgt ggacgcggaa cagtggtgcc ttgttgtaga
    11161 gaagcgggaa gccgagcaga cacggcgccg gctgcttgag gaggggcttc ttgaccggcg
    11221 gtataaaccg cgctccgaag gggatgcggt cctcctcccg gtgacggagg agatcgcggg
    11281 tgctacccgg cgcacctttg taaccgtgcc acagcgcgcc gatctccccc gccacgacct
    11341 cgtgggcggg atcgctatcc tgcaggagga cgatccggag gcggcaaaac ggctgcttgc
    11401 atcccggccc tcccttcaca cggtcctctt cccggagaca ccggtcgagg gggagtaccg
    11461 cacccgccgg ttcgccgtcc tcgccgggac tcctaccaca cgcacccggg tgacggagta
    11521 cggcctccgg ttcgacgtgg accttgccca cgcctacttc tcagcccgtc tctcgaccga
    11581 acggcagcgg atcctcggga tgatggagga cggggagcgg gtgctcgaca tgttcgccgg
    11641 cgtcgggccg tttgc
//
LOCUS       DAOSII010000060        11528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_5574_length_11528_cov_51.275255, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000060 DAOSII010000000
VERSION     DAOSII010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11528
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5574_length_11528_cov_51.275255"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            473..625
                     /locus_tag="PLV90_05965"
     CDS             473..625
                     /locus_tag="PLV90_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96698.1"
                     /translation="MVTITAPDATILIVGARDGTLITAVIDKPADLPTIQRRLLDIAV
                     RIGEEA"
     gene            625..1260
                     /locus_tag="PLV90_05970"
     CDS             625..1260
                     /locus_tag="PLV90_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOF96699.1"
                     /translation="MYTILIVDDSPMIVDVFATMLERGGYRPITAFSGEECLEVLKKT
                     PPDLVLLDIMMEPMDGWETLGKIKTSPATRGIPILMLTAKPLTPEEANEYGAYIEDYI
                     LKPTTHHQLYEAIERVLARRHSIAADIERAREAGVDPRVIDEYERLYKSVDINRRLLK
                     ILETTYNIKDARVGMGENITRAIKSMAASVKLQEERLNQVRAQFSDVFGKQ"
     gene            complement(1302..1733)
                     /locus_tag="PLV90_05975"
     CDS             complement(1302..1733)
                     /locus_tag="PLV90_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HOF96700.1"
                     /translation="MTSPRTHADTAISALSGATLAIYLLIALSLALLALASFYDVVLQ
                     AVALIRAENMTYGVLQVLHALLLTLIIVEILETVTAYFRTSRVQVTPILIAGITAMVR
                     RVLTFGVEPVEPLDVGMTLAAILVLTVAIVFIRRQDGTIYD"
     gene            1919..1992
                     /locus_tag="PLV90_05980"
     tRNA            1919..1992
                     /locus_tag="PLV90_05980"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1953..1955,aa:Lys,seq:ttt)
     gene            2072..2323
                     /locus_tag="PLV90_05985"
     CDS             2072..2323
                     /locus_tag="PLV90_05985"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit H"
                     /protein_id="HOF96701.1"
                     /translation="MGTPLDVLNHEMVPDHQIMGEEEVADLLATYHISLEQLPKIYHD
                     DPVIKAIGGSVGNVIRITRDSRTAGRAEAYRLVMKRPKK"
     gene            2366..3907
                     /locus_tag="PLV90_05990"
     CDS             2366..3907
                     /locus_tag="PLV90_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B''"
                     /protein_id="HOF96702.1"
                     /translation="MSRAYFSREHVARHQLDSYNNFLLHNLQKVVDEQRVIETDIETR
                     GKGKEAVWVELGRIEVKKPRVREADGSQSELFPSEARLRNLTYAAPIQLDMTLVQGEE
                     PQEPIVTIIGQLPVMVGSAACNLYSMSDAERTAHGEDPLDPGGYFIVNGTERVLMTLE
                     DLASNKIMTEFTERYNERIYVAKVFSQFRGYRALVIVERNKKNLLEVSFPSVAGHLRF
                     VDLMRALGLQGDHDIVEAVSTDEEILTFMMQNLEESECDTVDEGVMYVGKKLAPNQTR
                     DYQRKRAEFVLDNYLLPHLNYLMPVGIKEDDPGYRPAVEGVRLAKAHFLGRMAEACFD
                     LVLDRRRIDDKDHYANKRLKLAGDLMEDLFRISLNRLTRDVKYQLERASMRHRDLSIG
                     TAVRADVLTERLLHPLATGNWVGGRTGVSQLLDRVDHMAVLSHLRRVISPLSRSQPHF
                     EARDLHPTQWGRICPSETPEGPNCGLVKNFAQMVEISKGVVDEESVKNALYGMGVIAL
                     RRETA"
     gene            3904..5718
                     /gene="rpoB"
                     /locus_tag="PLV90_05995"
     CDS             3904..5718
                     /gene="rpoB"
                     /locus_tag="PLV90_05995"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B"
                     /protein_id="HOF96703.1"
                     /translation="MRQSRVFVDGALIGLVDDPRDLVGRMREMRRRGELSSEINVSYK
                     EFNNDVIIHTDRGRARRPLIVVRNGRPQVADDEIESLRRGEIKFENMVQIGAIEFVDA
                     EEEEDLFIAINEADLTPEHTHLEIDPSLILGIGAAHVPFPEHNASPRVTMGAGMIKQA
                     LGFGAANMKLRPDTRGHMLHYVQKPLTFTQTSDVIGSDDRPAGQNFVVAIISYEGFNI
                     EDALIINQASIDRGLGRSHFFRTYDGEERRYPGGQVDRIEIPDEEVSGAHGAEYYANL
                     DVDGIISPETVVREKDVLIGKTSPPRFLEEPTSELIAVEKRRDTSVTMRSNEHGIVDT
                     VIITEGENSSHLVKVRTRDLRVPEVGDKFASRHGQKGVIGLIQPAADMPFTESGMTPD
                     LIINPHAVPSRMTIGHMLEMLGGKVGSLEGRRIDATAFRGEREANLRRSLGELGFAHN
                     GREVMYDGVTGRQFAADIYIGVIYYQKLYHMVSSKMHARSRGPVQVLTRQPTEGRARE
                     GGLRFGEMERDVMIGHGAAMALKERLLDESDKVTQWVCAKCGMVAMLDRKRKVTRCLA
                     CGGETDIYPVEMSYAFKLLLDEMKSMGIAPRLRLDDMV"
     gene            5740..8394
                     /locus_tag="PLV90_06000"
     CDS             5740..8394
                     /locus_tag="PLV90_06000"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A'"
                     /protein_id="HOF96704.1"
                     /translation="MTSPKRVGRIEFGLFSPKEIRKMSVRKIIWADTYDDDGFPYPQG
                     LMDLNLGVIDPGLRCKTCDQKAADCPGHFGHIELAKPVIHVGYTRLIRKLLRVTCRSC
                     SRLLMTAEEVEKVVGPEDSEIAGDIVSEKDIKKERVCPHCDEQQLKINFEKPTTFSEV
                     FVEEGRKIEHKLTPADIRARLEKIPDDDLRVMGINPDVARPEWTILTVLPVPPVTMRP
                     SIILENGQRSEDDLTHKLVDIIRINQRFKENQDAGAPQLIIEDLWELLQYHVTTYLDN
                     EVAGCPPARHRSGRPLKTLSQRLKGKEGRFRGSLSGKRVNFSARTVISPDPNLSITEV
                     GIPLAIANEMTVPVRVTPFNIDEMRQYVLNGPERPTIRSPCGANYAIRPDNRRMRLSD
                     ANLETVKEMLEVGWTVERQLKDGDIVLFNRQPSLHRMSIMAHRIVVMDGKTFRLNPAV
                     CPPYNADFDGDEMNLHIPQTEEARAEAEILISVQANILSPRTGGPIIGGIHDHISGIF
                     LLTHRVRHFGKEDALYLTQHLPLEDLGLPEPDRHDDAGHPLWTNKQIFSLILPNDLNM
                     IFRASSCQNCETCRREMCENDAFVRIAAGNLVSGTIDKKAIGAFDGQILHRIIRQHGM
                     KRAARFIDDVTKLSIRGIMLEGFSFGIDDEDLTRTEYGQIDEALKGAVGDVDRRIKIF
                     KEGQLEPMPGRTLEETLEMQIMQVLGKARDRTGEIAGRHLGMDNSAVVMAVSGARGSM
                     LNLTQMAGCLGQQSVRGERIMRGYEDRTLPHFRRNELGAEAHGFITNSYKSGLSPTEF
                     FFHAIGGREGLVDTAVRTSQSGYLQRRMINALQDLKVEYDGTVRTTGGRIIQFCYGED
                     GTDPARSSYGAPVDVKGIVESVLKEEIK"
     gene            8391..9563
                     /gene="rpoA2"
                     /locus_tag="PLV90_06005"
     CDS             8391..9563
                     /gene="rpoA2"
                     /locus_tag="PLV90_06005"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A''"
                     /protein_id="HOF96705.1"
                     /translation="MNSDMEQRIDALDLPYRTRVDLKASLADRDITDEQFDRILGMIF
                     SEYEKSRIEPCEAVGIVAAQSIGEPGTQMTMRTFHYAGVAEINVTLGLPRLIEIMDAR
                     REPSTPTMAVYLQDDWAFNRDRAREVSWQIEAAPLHEFGDITIDMEKMQILVLLNKSV
                     CDRRKIGLEEIFEAAPKKIREKRHFRDFDTEEDVKKASIAFIPKNRESYQNLFQLAEH
                     VRNVIVQGIDDIERVVVRKEGGEYILYTEGSNLRDVFEVEGVDTSRTRSNNISEIADV
                     LGIEAGRNAIIQEALSTLNEQGIGVDVRHIMLVADMMCMDGEVKQIGRHGIAGEKESV
                     LSRAAFEVTVNHLLDAAIANEVDELNGVTENVIVGQPIQLGTGDVKLIAKPVNLKI"
     gene            9575..9862
                     /locus_tag="PLV90_06010"
     CDS             9575..9862
                     /locus_tag="PLV90_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30e"
                     /protein_id="HOF96706.1"
                     /translation="MDFNASLRKAVKTGTVFLGQNKTRECIEEGRARLVVVAKNSPES
                     VKNLVNEIDIPVYVYEGSSVQLGKACGMPYVVSALAVVEPGESDILNAARV"
     gene            9866..10315
                     /locus_tag="PLV90_06015"
     CDS             9866..10315
                     /locus_tag="PLV90_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusA-like transcription termination
                     signal-binding factor"
                     /protein_id="HOF96707.1"
                     /translation="MPQVVLTEECMRLISQFESLTGAGSRDCVVDNRNERIIFVINPG
                     DMGLAIGKSGSSIKKASEVMGKRIEVVEYSTDPAQFLRNCFLPAQVTGVDFDTNEEDQ
                     QVAHIEVRSEDRGLAIGKAGKNIFKAKVLALRQHDIADVVLMQNEAA"
     gene            10553..>11528
                     /locus_tag="PLV90_06020"
     CDS             10553..>11528
                     /locus_tag="PLV90_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96708.1"
                     /translation="MPGGVLILHLLAAAAIAALCLGSIASCAAAAPVPGENDTPGVPP
                     APGTIAGEKPVTAELPGTPDEPSEPAETSVTARPADIAATLGETPPSDIINATPSLSA
                     TATPEPVVSSPDGADGEETPVPTLEDSNVTASPEPTVSTLSTADDVDDEDEPEETPVP
                     VPTLEREAPPRPNATMAKSHQYHDRPLAQGAHPLVRPTSPPETNETVEQPEDVTPREG
                     LFVRGVGVLLTRTPEDVALTRSGVETANLDWLLDTAMRLGGRRPRVAMEGETFTVTVT
                     VEAQSLAVTGEEPAYVVLVPPPGEVAVYDITRTKEPRNLLDGERAVWEF"
BASE COUNT         2483 a         3472 c         3550 g         2023 t
ORIGIN      
        1 gccagcaccg gcgcataaag gcggggatcg gcaaagtcgt gcaccgtgga gtgccatgag
       61 tcaacctcct ggaacagccc ccgtgcccgc gaggatgttg cggcagcgcc ctgctccccc
      121 ggcgatcgcg gtgctctctg aatcctgaca aaatacagga cagcggcacc ttcggggcga
      181 taccgattcg atattcatat atgatgcggc gcagatatct ccatggagat ataccggggt
      241 cagtcgtatg agttccaatc ccttcaagga ggagaatgct caaccgtgca tcgatgaggt
      301 tcgggcaaca cctggtgtcg ccgcatgcgc cctcgtatcc ggtgaaggtg cgatcctggg
      361 taaatatttc cgggagggca accgctcatc atccctcttc gcggtgatgt gtgcaacggt
      421 tctcgcatct gcggaggctg catgcggcag cgtccacctg cagagccctt ccatggtcac
      481 catcaccgca cctgacgcta ctatccttat cgtgggtgcg agagacggca ccctgatcac
      541 agcggtaata gataaacctg cagacctccc aacaatacag aggcggttat tggatatagc
      601 agtcaggatc ggagaggagg cgtgatgtac acaatactta tagtcgacga tagccccatg
      661 atcgtcgatg tttttgccac catgctggaa cgtggcggtt atcggccaat caccgccttt
      721 agcggcgagg aatgcctgga agttctgaag aagacccccc ctgacctggt tcttctggac
      781 atcatgatgg agccgatgga tgggtgggag acactcggaa agatcaagac cagccctgcg
      841 acacggggca tccctatcct catgctgacc gcaaagcccc tcacgcccga ggaggcaaac
      901 gaatacggtg cttacatcga agattatatc ctcaaaccca caacccacca ccagctctat
      961 gaagcgatcg agcgtgtcct ggcgcgccgg cattccatcg ctgccgatat cgagcgggcg
     1021 cgcgaggccg gcgtggaccc tcgggtgatc gacgagtacg agcgcctcta caagagcgtc
     1081 gatatcaatc ggcgcctcct caagatcctc gagaccacct acaacatcaa ggatgcccgg
     1141 gtgggtatgg gcgagaacat aacgagagcc ataaagagca tggcagcgag tgtaaaactc
     1201 caggaagaac gcttaaatca ggtccgcgcc cagttttcag atgttttcgg gaaacagtga
     1261 ccactccctc ccacccctca aagggcagcg gccggtcgcc tctaatcgta gatcgtgcca
     1321 tcctgccgcc tgatgaagac gatcgccacg gtcagcacga ggatggcggc aagcgtcatg
     1381 ccgacatcga ggggctcgac aggttcgacc ccgaaggtca acacccgccg caccatcgcc
     1441 gtgatgcctg cgatcaggat cggcgtcacc tgcacccgac ttgtcctgaa gtatgccgtc
     1501 accgtctcaa ggatctccac gatgatgagg gtcaggagga gcgcgtgcag cacctggagc
     1561 accccatacg tcatgttttc agcgcggatg agcgccaccg cctgcaggac gacatcgtag
     1621 aaggaggcga gcgcaagcag ggcgagcgag agggctatga gcaggtatat ggcgagtgtt
     1681 gcccctgata gggcagatat ggcagtatcg gcatgagtcc tgggtgaagt catgtgggcg
     1741 cccccgtatg aagggtagag aaacaaacta tcccccgggc attaaatatt ccgataggca
     1801 tgcaatggcg atgaattgaa aggtttatat cgttagactg tgaataatat aatacgtctg
     1861 caagtaagtt atatatacta ttcttgcatt gtaataatgc tggatggcag tacgcgcggg
     1921 gcctatagct cagttaggta gagcgcctgg cttttaacca ggtggtcggg ggttcaaatc
     1981 cccttgggcc cgttgccatg ggcgatgtct atggatttta acaaggtgat gcatctgata
     2041 cacaactttt tcgtcggtgg ggtgtaaggc gatgggcact ccacttgacg tactcaacca
     2101 tgagatggtt ccggaccatc agattatggg cgaagaagag gtcgcggatc ttcttgcgac
     2161 gtaccatatc tctttagaac aattgccaaa aatttaccat gacgatcctg taatcaaagc
     2221 tatcgggggc agtgtcggga acgtcatccg gatcacccgt gacagccgca cagcaggcag
     2281 ggccgaagcc taccgccttg tcatgaagag accgaagaaa taaatcaagg gccgggagtt
     2341 gatccatctg ttagatcgaa gtgttttgtc gagggcatac ttttcgcggg agcatgtagc
     2401 acgccaccag ttagactctt ataacaactt cctcctgcac aacctccaga aagttgtcga
     2461 tgaacaacgt gtaattgaga ccgatatcga gacccgtggc aaaggcaagg aggccgtatg
     2521 ggttgaactg ggcaggatcg aggtgaagaa acctcgtgtc cgcgaggcgg acgggtcgca
     2581 atccgaactc ttcccgagcg aggcgcgcct ccggaacctc acgtacgcgg cacccattca
     2641 gctcgacatg acgcttgtcc agggcgagga gccgcaggag ccgatcgtca ccatcatcgg
     2701 gcagttgccg gtgatggtgg ggtcggccgc ctgcaacctc tatagcatga gcgacgccga
     2761 gcggaccgcg cacggtgagg accctctcga ccccggcggc tacttcatcg tcaacggcac
     2821 cgagcgggtg ctgatgacgc ttgaggacct cgcctcgaac aagatcatga ccgagttcac
     2881 cgagcgctac aacgaacgga tctacgtagc gaaggtcttc tcgcagttcc ggggataccg
     2941 ggcgctcgtg atcgtcgagc ggaacaaaaa gaacctgctt gaagtttcgt ttccctcggt
     3001 cgccgggcac ctccgctttg tcgacctgat gcgggcgctg gggctccagg gtgaccacga
     3061 tatcgtggaa gcggtctcga ccgacgaaga gatcctcacc ttcatgatgc agaacctgga
     3121 agagagcgag tgcgacacgg tcgacgaagg cgtcatgtac gtcgggaaga aactcgcccc
     3181 gaaccagacc cgggactacc agcgcaagcg ggcggagttc gtcctggata actacctcct
     3241 gcctcacctg aattacctga tgccggtggg cataaaagag gatgaccccg gctaccggcc
     3301 ggccgtcgaa ggcgttcgcc tggcaaaagc gcacttcctc ggccgcatgg ctgaggcctg
     3361 ctttgacctg gtgctcgacc ggcgccggat cgacgacaaa gaccactacg cgaacaagcg
     3421 gctgaaactt gccggcgacc tgatggaaga cctcttccgg atatcgctca accgcctgac
     3481 gcgcgacgtg aagtaccagc ttgagcgggc cagcatgcgc caccgcgacc tctcgatcgg
     3541 cactgcggtg cgcgcagatg tcctgaccga acgactgctc cacccgcttg caaccggcaa
     3601 ctgggtgggt gggcgcaccg gcgtatcgca gctccttgac cgggtcgacc acatggcggt
     3661 gctctcgcac ctccggcgcg tcatctcccc gctctcccgc tcgcagcccc acttcgaggc
     3721 ccgtgacctg caccccaccc agtggggccg gatctgtccg agcgagacac ccgaggggcc
     3781 gaactgtggt ctggtgaaga atttcgccca gatggtcgag atcagcaagg gcgtcgtcga
     3841 tgaagagagc gtgaagaacg ccctctacgg tatgggcgtc atcgctctcc ggagggagac
     3901 ggcatgagac agtcaagggt ctttgtggac ggcgccctga tcgggctcgt agacgaccct
     3961 cgcgatcttg tcgggcggat gcgcgagatg cgccggcgcg gcgagctctc gtccgagatc
     4021 aacgtctcct acaaggagtt caacaacgac gtcatcatcc acaccgatcg cgggagagcg
     4081 cgccgacccc tgatcgtggt caggaacggc agaccacagg tagccgacga tgagatcgag
     4141 tccctccgga gaggcgagat aaagtttgag aacatggtgc agataggcgc gatcgagttc
     4201 gtcgatgccg aggaggagga agacctcttc atcgccatca acgaggcgga cctcaccccc
     4261 gagcacacgc accttgagat cgacccctcg ctcatccttg gtataggggc ggcgcatgtt
     4321 ccgttccccg agcacaacgc gagtccccgt gtcaccatgg gtgcagggat gatcaagcag
     4381 gcgctcggat tcggggcggc gaacatgaag ctccgccccg acacccgggg gcacatgctc
     4441 cactacgtcc agaaacccct caccttcacc cagacatccg atgttatcgg ttccgacgac
     4501 cgacctgcag gccagaactt cgtcgtcgcg atcatatcct acgaaggatt caacattgaa
     4561 gatgcgctga tcatcaacca ggcctccatc gaccgcggtc tcgggcggtc acacttcttc
     4621 cgcacctacg acggcgagga gcggcggtac cccggcggtc aggtcgacag gatcgagatc
     4681 cccgacgaag aagtctccgg cgcgcatggc gcggagtact acgcgaacct ggatgtcgac
     4741 ggcatcatca gccccgagac ggtcgtccgc gagaaggacg tgctgatcgg gaagacgtcc
     4801 ccgccgcggt tccttgagga accgacgagc gagctcatcg ccgtcgagaa gcggcgcgat
     4861 acgtcggtca cgatgcggag caacgagcac ggcatcgtgg acaccgtcat catcaccgag
     4921 ggcgagaact cctcgcacct cgtgaaggtc cggacccgtg acctccgggt gccggaggtg
     4981 ggcgacaagt tcgcatcccg gcacggccag aaaggcgtca tcgggctgat tcagcctgcg
     5041 gcggatatgc cgttcacgga gtcggggatg acgcccgacc tgatcatcaa cccccacgcg
     5101 gtcccgagcc gtatgaccat cgggcatatg ctcgagatgc tgggcggcaa ggtcggttcc
     5161 ctcgagggcc ggcggatcga cgcgacggcg ttccgcggtg agcgcgaggc aaacctccgg
     5221 agatcgctcg gggaactcgg gtttgcgcac aacggccgcg aggtgatgta cgacggcgtc
     5281 accgggcggc agtttgcggc cgatatctac atcggcgtca tctactacca gaaactctac
     5341 cacatggtct caagcaagat gcacgcgcgg tcgcgcggcc ccgtgcaggt tctcacccgg
     5401 cagccgaccg aagggcgtgc gcgcgagggc ggtctccggt tcggtgagat ggagcgggac
     5461 gtgatgatcg gccacggtgc ggccatggcc ttaaaagagc ggctgctcga tgaatcggac
     5521 aaggtgaccc agtgggtctg cgcgaagtgc ggcatggtgg cgatgcttga ccggaagcga
     5581 aaggtgacgc gctgtcttgc ctgcggcggc gagaccgata tctaccccgt cgagatgagc
     5641 tacgccttca aactcctcct cgatgagatg aagagcatgg gcatcgctcc ccgcctgcgg
     5701 ctcgacgata tggtataaga gagggggcgc aatcagatta tgaccagtcc aaaacgtgtt
     5761 ggaaggatcg agttcgggct cttctccccg aaggagatcc gtaagatgag tgtcaggaag
     5821 atcatctggg cggacaccta cgatgacgac gggttcccct acccgcaggg cttgatggac
     5881 ttaaacctcg gggtcatcga ccccggtctt cggtgcaaga cctgcgacca gaaggctgcc
     5941 gattgtccgg gccacttcgg acacatcgag cttgcaaaac ccgtcatcca tgtcggctac
     6001 acccggctaa tccgaaagct cctcagagtg acctgccggt cctgctcgcg gctgctgatg
     6061 acggccgaag aggtcgaaaa ggtcgtcggg cccgaagaca gcgagattgc cggcgatatc
     6121 gtctccgaga aggatatcaa gaaagagcgg gtctgccccc actgcgacga gcagcagctc
     6181 aagatcaact tcgagaagcc caccaccttc tctgaggtct tcgtggagga agggaggaag
     6241 atcgagcaca agctgacccc ggccgacatc cgtgcgcgcc tggagaagat cccggacgac
     6301 gacctgcggg tgatggggat caatcccgac gtcgcgcggc cggagtggac gatcctcacc
     6361 gtcctcccgg tcccgccggt cacgatgcgc ccctcgatca tcctcgagaa cgggcagcgg
     6421 tccgaggacg acctgaccca caaactcgtg gatatcatcc ggatcaacca gcggttcaag
     6481 gagaaccagg atgccggcgc accccagctg atcatcgagg acctctggga gctcctgcag
     6541 taccacgtca ccacctacct cgacaacgag gtggcggggt gcccgcccgc ccgccaccgg
     6601 agcggccgtc ccttaaagac tctctcgcag cgactcaagg ggaaggaagg gcgattccgc
     6661 ggctcgctct cgggaaaacg tgtgaacttc tccgcccgta cggtgatctc ccccgatccg
     6721 aacctctcca tcaccgaggt cggtataccg cttgccatcg caaacgagat gaccgtcccc
     6781 gtgcgggtga cgccgttcaa catcgatgag atgcggcagt acgtcttgaa cggccccgaa
     6841 cgccccacca tccggtcgcc ctgcggcgca aattacgcga tccggcccga caatcggcgg
     6901 atgcgcctct ccgacgcgaa tctcgagacg gtcaaggaga tgcttgaggt aggctggacc
     6961 gtggagcgcc agctaaaaga cggggatatc gtcctcttca accggcagcc ctcgctgcac
     7021 cggatgagca tcatggccca ccggatcgtc gtgatggatg gaaagacctt ccggctgaat
     7081 ccggccgtct gcccgcccta caacgccgac tttgacggcg acgagatgaa cctgcatatc
     7141 ccgcagaccg aggaggcgcg ggccgaggcc gagatcctga tatcggtgca ggcaaacatt
     7201 ctctcgccga ggaccggcgg tccgatcatc ggcggcatcc acgaccacat atcgggcata
     7261 ttcctcctga cccaccgggt ccgacatttc ggaaaggagg atgccctcta cctcacccaa
     7321 cacctgcccc tcgaggatct gggcctgcct gaacctgacc ggcacgacga tgccgggcac
     7381 ccgctctgga ccaacaagca gatcttctct ctgatcctcc ccaacgacct gaacatgatc
     7441 ttccgggcct cgtcgtgcca gaactgcgag acctgccggc gggagatgtg cgagaacgac
     7501 gcgttcgtcc ggatcgccgc cgggaatctg gtctccggga ccatcgacaa gaaggcgatc
     7561 ggcgcgttcg acggccagat cctgcaccgg atcatacggc agcacggcat gaagcgggcc
     7621 gcccggttca tcgacgacgt gaccaaactc tctatccggg gcatcatgct cgaagggttc
     7681 tcgttcggta tcgacgacga agacctgacc cggaccgagt acggccagat cgacgaggcc
     7741 ctgaagggcg ccgtcggcga cgtcgaccgc cggatcaaga tcttcaagga agggcagctc
     7801 gaaccgatgc cgggccggac gcttgaggag acgcttgaga tgcagatcat gcaggtgctc
     7861 ggcaaggccc gtgaccgcac cggtgagatc gccggacgcc acctcggcat ggacaacagc
     7921 gccgtggtga tggcggtctc cggcgcccgc ggatcgatgc tgaacctgac gcagatggcc
     7981 gggtgtctcg gtcagcaatc ggttcgcggc gagcggatca tgcgtggcta cgaggaccgg
     8041 accctgccgc acttccggag gaacgagctc ggtgcggagg cgcacgggtt catcaccaac
     8101 agttacaaga gcggcctctc ccccacggag ttcttcttcc acgccatcgg cgggcgagag
     8161 ggtcttgtgg acaccgcggt ccggacgtcg cagagcggct acctgcagcg gcggatgatc
     8221 aacgccctgc aggacctcaa ggtcgagtac gacggcaccg tccggacgac gggcgggcgg
     8281 atcatccagt tctgttacgg agaggacggg accgatccgg cacggagcag ctacggcgcc
     8341 ccggtggatg tcaaaggcat cgtcgagagc gtcttaaagg aggagatcaa gtgaacagcg
     8401 atatggagca gcggatcgat gccctcgatc tgccctaccg gacccgggta gacctcaagg
     8461 cgagtcttgc agaccgtgac atcaccgacg agcagttcga ccggatcctt ggcatgatct
     8521 tctctgagta cgagaagagc cggatagaac cctgcgaggc ggtcggtatc gtggcggcgc
     8581 agtcgatagg tgagcccggc acccagatga cgatgcgaac cttccactac gcgggtgtgg
     8641 ccgagatcaa cgttaccctc ggtcttcccc gcctcatcga gatcatggat gcccggcgcg
     8701 aaccgagcac ccccacgatg gcggtctacc tgcaggacga ctgggcgttc aaccgcgacc
     8761 gcgcccgcga ggtgagctgg cagatcgagg ccgcgccgct ccatgagttc ggcgatatca
     8821 ccatcgatat ggagaagatg cagatccttg tcctgttgaa caagtcggtc tgtgaccggc
     8881 gcaagattgg ccttgaggag atctttgagg cggccccgaa aaagatccgc gagaaacggc
     8941 attttcgtga cttcgatact gaagaggacg taaaaaaggc gtcgattgcc ttcataccga
     9001 agaaccggga gagttaccag aacctctttc agcttgcaga gcacgtcaga aacgtcatcg
     9061 ttcagggtat cgatgacatc gagagggtcg tcgtcagaaa ggagggcgga gagtatatcc
     9121 tttatactga aggctccaac ctaagagatg tcttcgaagt cgagggagtc gatacctccc
     9181 gcacccggag caacaacatc agcgagattg ccgatgtgct cggtatcgag gcggggcgga
     9241 acgcgatcat tcaggaagcc ttaagtacct taaacgagca gggcatcggc gtcgatgtcc
     9301 gccacatcat gctcgtcgcg gatatgatgt gcatggacgg cgaggtcaag cagatcggcc
     9361 ggcacggtat cgccggcgag aaggagagcg tcctctcccg ggctgcattc gaggtgacgg
     9421 tcaaccacct gctggatgcc gcaatcgcaa acgaggtgga tgaattgaac ggcgtcaccg
     9481 agaacgtgat cgtgggccag cccatccagc tcggcaccgg tgatgttaaa ctcatagcaa
     9541 aacctgtaaa cctgaaaatc taggagaacc atacatggac tttaatgctt cacttcgtaa
     9601 agccgtgaag actggtacag tattcctcgg ccagaacaag acccgggaat gcatcgagga
     9661 gggcagggcc agacttgtcg tggtggccaa aaacagcccg gaatccgtca aaaatcttgt
     9721 gaatgagatc gatatccctg tttacgtcta cgagggctcg agtgtccagc tcgggaaagc
     9781 ctgcggtatg ccgtatgtcg tcagcgcgct tgccgtggtc gaaccaggtg aatccgatat
     9841 cctgaatgcc gcgagagtgt agatgatgcc gcaggtcgtg ctgactgagg agtgcatgcg
     9901 ccttatctct cagttcgaga gcctcaccgg tgcaggcagc cgcgattgtg tcgtcgataa
     9961 ccgcaacgag cggatcatct tcgtgatcaa tcccggcgac atgggacttg ccataggcaa
    10021 gagcggatcg agcatcaaga aggcatccga ggttatgggc aagaggatcg aggtcgtgga
    10081 atactccacg gatccggccc agttcctccg gaactgtttc cttcccgccc aggtcaccgg
    10141 cgtcgacttc gatacgaacg aggaggacca gcaggtcgcc cacatcgagg tgcggagcga
    10201 ggaccggggt cttgccatcg gtaaggcggg gaagaatata ttcaaggcaa aggttcttgc
    10261 actgcgccag cacgacatcg ccgatgtcgt gttgatgcag aacgaggctg cctgaacctt
    10321 tctccccttc ttttcaatca acccgcatcc gtaccccccc atccggaatg gcgatccccg
    10381 actgccgggg gtcctctcgc agggctgatc cgcacaggag gttccgggcg agccctgata
    10441 cggaaattat ttatactcgt gcgcgcccca tggtccaacc atgagcggag gatccgggcg
    10501 gatggtgggg cctccgggta gaacccgggg agagggcaga gggagtttgc gaatgccggg
    10561 aggggtgctc atcttgcacc tcctcgccgc cgccgcgata gcggcgctct gccttggctc
    10621 gatcgcctct tgtgcggctg cagccccggt tcccggggag aacgatactc ccggagtgcc
    10681 gccggcaccc ggcaccattg ccggcgagaa acccgtcacc gcagaactgc cgggtacccc
    10741 ggacgaaccg tcggagccgg cagaaacgtc tgtaacggcg aggcctgcgg atatcgcggc
    10801 cactctggga gagacgccgc cctccgacat catcaacgcg acaccttccc tgtcggcgac
    10861 agccactcct gaacctgttg tttcgtcccc cgatggcgcc gacggtgagg agacgccggt
    10921 gccgaccctg gaggactcga atgtgaccgc ttctcccgag cctaccgtgt cgaccctctc
    10981 gacggccgat gacgtcgacg atgaggatga accggaggag acgccggtgc cggtgccgac
    11041 cctggagcgg gaggctcccc cgaggccgaa tgcgacgatg gcgaagtcgc accagtacca
    11101 tgaccgcccg ctggcccagg gtgcccaccc gctcgtcagg cctacatcgc cccctgagac
    11161 gaatgagacc gtggagcagc ctgaggacgt tacgccgcgc gaaggcctct tcgtcagggg
    11221 cgtcggggtt ctcctcaccc ggacccccga agacgtcgcc ctcacccgga gcggggtgga
    11281 gacagcgaac ctcgactggc tgctcgatac ggccatgcgt cttggcggtc ggcgcccacg
    11341 ggtggcgatg gagggcgaga cgttcaccgt cacggtgacg gtcgaggcgc agagcctggc
    11401 ggtcacggga gaggaaccgg cgtacgtcgt tctggtgccg ccgccgggcg aggttgccgt
    11461 ctatgacatc acgcggacaa aagagccccg gaacctcctg gatggggaac gggctgtctg
    11521 ggagttct
//
LOCUS       DAOSII010000061        11405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_5635_length_11405_cov_34.907577, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000061 DAOSII010000000
VERSION     DAOSII010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 11405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11405
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5635_length_11405_cov_34.907577"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..1350)
                     /gene="gatA"
                     /locus_tag="PLV90_06025"
     CDS             complement(49..1350)
                     /gene="gatA"
                     /locus_tag="PLV90_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOF96709.1"
                     /translation="MAGTLDFSPDDRYNAFITTCSTAPFSGGALAGIAVAVKDNISTA
                     GIETTCGSRILEGYIPPYDAYVVELLRREGAAIVGKTNMDEFGMGSTTETSAFGPTKN
                     PVDPSRVPGGSSGGSAAAVAAGLVPMALGSDTGGSIRCPAAFCGVVGLKPTYGRVSRY
                     GLIAYASSLEQVGPMARTVEDVSRVMSVIARYDPRDATMFDRPYDHRPSADIGGLRVG
                     IPGEYFGEGVDPRVAKKVRDAIGVIEDLGASTVPVSIPGMRYALAAYYVICTAEASSN
                     IARFDGVRYGPAVDTRKSWHEAYQDIRQEKLGPEVRRRVMLGTFALSAGYAGRYYAKA
                     LGARQKIRQDFERAFRDVDVIAGPTMPTVAFPLGERTDPVSMYLSDILTAPANLAGIP
                     AISVPCGRVDGLPVGLQLMGRQFEEERIIDAAYAYEQGVKA"
     gene            complement(1351..1620)
                     /gene="gatC"
                     /locus_tag="PLV90_06030"
     CDS             complement(1351..1620)
                     /gene="gatC"
                     /locus_tag="PLV90_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOF96710.1"
                     /translation="MVTESDIEHIAELADIGISRDEVPEFTRQFNAILEYFEILDTVE
                     GESAPAGGITNAFRDDEPRPCLSQEAALANAGSTEDGFIKAPRVM"
     gene            complement(1610..2608)
                     /locus_tag="PLV90_06035"
     CDS             complement(1610..2608)
                     /locus_tag="PLV90_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HOF96711.1"
                     /translation="MNLKGWIERDGVRLSPADVERMLREEPAALARCGGEFLLEWDDC
                     IARDTFGIMPGQIAPGTICCGGREAARIAPDPPPCGLEEAIVTAVGLRSNEGAVAFSG
                     GVDSALIAGLAGLPCVAVGLRGSHDALWAAAAARMMGLDLAIVTPTEEEVAEALREVV
                     RVIPDPTNPIDASIAATLSFVAAWAGENGHTRILAGQGADELFGGYARYLSSPDLAAE
                     LERDVMDHGRQRARDQAVAALHGAYFSMPYIDTRVVRAARAIPAGEKVRGGVRKHPLR
                     TVAERHIPAEIARAEKKAMQYGSGIWRTMHQLTRQNGYKKSVQGYLTEISRAEHGN"
     gene            complement(3131..3793)
                     /locus_tag="PLV90_06040"
     CDS             complement(3131..3793)
                     /locus_tag="PLV90_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96712.1"
                     /translation="MAQRTYPGHTLIGNIATTKRRAKGDVMRQLKQERQADPGAESWL
                     LMFQGEYQPGYWITLLARKNATLKAIDTELRYVWLECCSHLSAFTIYGEEYSSYPDRW
                     TTEKGMNVPLGELFSAGLTGKYIYDYGDSTYLTFKVLDLVPYAPAGGKDIELVARNDP
                     PDIRCSVCGKPATTICLECLDESPGDAFLCDDCFGEHECDEEMSLPVVNSPRMGQCAY
                     TG"
     gene            3987..4556
                     /locus_tag="PLV90_06045"
     CDS             3987..4556
                     /locus_tag="PLV90_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF96713.1"
                     /translation="MFGVSTDAMLQSVIVFPSAFLIGAYTDLSVFVQALPIMLLITVG
                     LTFSSLVKSFEGFGLIQTFINLPIFFLNGALFLLSNVPQWLLWINRSNPLTYGVDALR
                     TVMMGGHAPLLPMYVNIGVLRASDAVMFTIGIYAFLGRNNHQLLLTLVGGSSTHDPEF
                     IPPTSADDGRSPWSPQRPRQNGDLLIAEK"
     gene            4666..5577
                     /locus_tag="PLV90_06050"
     CDS             4666..5577
                     /locus_tag="PLV90_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOF96714.1"
                     /translation="MVNAVLLVIAGVILANILAETGIFSRLSSFTGPLCRFSGLSDAC
                     VLSIVAMGVNATAGKSMLAEYYRDGKVTEREVIPVLIMGAFPVVLGESLFRVQLPTAL
                     VLLGPVVGGVYTGLNLFSSGIQALFALLYSRRFLAGSGRAPPPVPSAAALMLNREVVI
                     SGCRKAVPTLRRVVPVTLAALVAFSLLSATGVMDGIAAAFDPLLRAVGLPGESAAALV
                     AQFLHFSAGYAIVASLLDGGVLTFETALVTLVIGSMVVITMIYVKYSVPLYLSLFGRF
                     GVRISLATYAASMAAKVITVALVFLLF"
     gene            5704..6261
                     /locus_tag="PLV90_06055"
     CDS             5704..6261
                     /locus_tag="PLV90_06055"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOF96715.1"
                     /translation="MREAGLSELFKTEERIRILRYVAGQRTVTATAVVEATGTSKALV
                     SRYLHLLVREEFCTRHGRMYIWQENARSLATKRLLNIDLLRAQVPLPEWARGIGVYGS
                     YAEGTNTAESDIDLWVFVDEYTPKLEICAARIEKTVSVASGTEVHILILTPEKLAELR
                     EADTPFYAGLMRWGITIGGASIGND"
     gene            6248..6703
                     /locus_tag="PLV90_06060"
     CDS             6248..6703
                     /locus_tag="PLV90_06060"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOF96716.1"
                     /translation="MATIEDCFSKKLIRRVVPSREKANESLNLASSYLCEARQVAGIG
                     ANRMSLAGAYMAWFHAARAVLFRDGIREKSHYCIELYLETYVRSGDLEDEWVLMFGRI
                     RAQRHESQYSFGPTPTDAEVSAAVLHAERFIERMRQLLSETPKKEWVGR"
     gene            complement(7466..8539)
                     /locus_tag="PLV90_06065"
     CDS             complement(7466..8539)
                     /locus_tag="PLV90_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96717.1"
                     /translation="MRYRHATTLILLLALTIGIAAGEDVPPTFPHDFWGGVTIDGSPA
                     PAGTEITALIGDTACGSIKTTKIGEYGSSSRHEGGRLVVSRTDGQSGETITFLINGRT
                     ARETATFTPDSTTRLDLSVAGGSTPPSGNGGGGPSGSSPAPVGTSAPSAPTVPVGRAS
                     LPLSETGEVSESVTVGTADGAGSLTVGKGTVARDKDGNPLGEVTIARADAAGLPPVPP
                     GATIGFALDCGPAGATFDPPATLTYTLSEEEWGRIGNSATLRVMWYNPGTGAWQEVPA
                     TVDPATRTVTAEVSHFSLFALTWAAASPAEATTGPAGTAPGDVPPGSEQPTGGETPWA
                     LIAAGVLVVAAVLATGWYLLKKR"
     gene            complement(8542..>11405)
                     /locus_tag="PLV90_06070"
     CDS             complement(8542..>11405)
                     /locus_tag="PLV90_06070"
                     /inference="COORDINATES: protein motif:HMM:NF023252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOF96718.1"
                     /translation="TYVPNGGSYKTTLATLAVKYPEAPLPSAPVANFTANVTAGDAPL
                     TVQFNDTSIGSPTSWLWDFGDNTTSDQQHPVHTYTTAGTYSVNLTVTNAAGSDSEVKT
                     GCITVSDPAGGDAPDLAASTLSSNNGEVFSASENTYTATITNIGTGDAGAFDVKFNVS
                     GAAGTVAVDALATGAGTTVTWTDETIRNAGDVVTITVTADPDGLIAESNEENNLASIE
                     KTVVHNGYRGMRWTDGGDIATAVTYDIRGDFLWSAGDGAYLSASTYPNWTTYTVNWTG
                     GDLAVPEGATIAGARLYVPYTWGKGPIFPSNVTLAFNSETVDRSAFYEDEKMWGSSYP
                     YGMTVYDVTDAFSAAGNTAVLTNTFPGGGNVSIRGMLLAVVYDDGTTAPHTIFMNEGF
                     DLLYGGEAQGTTPEQATAYAPFFGVETDDVLSAALITVAPGAGPARGDLLINDERLAD
                     VWSFTGTSQIGVDERDVTALLRTGENLVGFQSSADYMEAAAAFLVLEYPALPGSILVT
                     STPAGAAVWLDDEDTGEVTPITLEDVLAGDHVVTLKLGGYADASKSVTVVSDETVTVD
                     LELTTLTGSLEVTSTPEGAAILIDGADTGEVTNATLDGIGVGEHTITLRKAGYADAVA
                     EVTIAENETTTLHLDLIEAPGSIAVVSMPEGAAIFLDGADTGKLTNALLENVAAGEHT
                     VTVKLAGYIDASRTVTVTDNETITINFLLTEPAGSIAVTSTPDGARIFLDGTDTGEVT
                     NATLMNVAVGKHTIRLEIDDYLDVEEIVTVTSGEIASVHFTLDASAITLQPGWNFVST
                     PKRLADGSNTIAVFDGVDTNGRPVLFYNGTCQWEDLSREDAFRPLDGIWIYSNGTYEI
                     PLSFAAASTSPPPTKYLDAGWNAIGFSDTVPEPAVTTLRSVETAWANLFGWKAREQAY
                     DDSIIRGATGKHGEMRELSPFQGYWLYMNNADTLAAIGA"
BASE COUNT         1896 a         3432 c         3801 g         2276 t
ORIGIN      
        1 tcgtcgccgt cgcgagctgg acgtggatct caagcccgac gatcgtcttc atgccttcac
       61 cccctgctcg taggcatagg ccgcatcgat gatgcgttcc tcctcgaact gtcttcccat
      121 caactggagg cccacgggga ggccgtccac cctgccgcac gggaccgata tggccggtat
      181 cccggcaagg ttcgccggcg ccgtgagtat gtccgagagg tacatcgaga ccggatcggt
      241 cctctcgccg agggggaagg ccacggtcgg catcgtcggg ccggcgatga cgtccacgtc
      301 gcggaaggcc cgctcgaagt cctgccggat cttctgccgg gcccccaggg ccttcgcgta
      361 gtacctgccg gcatagccgg ccgagagggc gaatgtgccg agcatgacac ggcgccggac
      421 ctcggggccg agtttctcct gccggatgtc ctggtaggcc tcgtgccatg acttccgggt
      481 atcgaccgcc gggccgtagc ggacgccgtc gaaccgtgcg atgttcgagg aggcttcggc
      541 tgtgcagatg acgtagtagg ccgcaagggc ataccgcatg ccgggtatgc tcaccggcac
      601 cgtgctggca ccgaggtctt cgatgacacc gatggcgtcg cggaccttct ttgcgacgcg
      661 ggggtccacg ccttcgccga agtactctcc cggtataccg acacggagcc ccccgatatc
      721 ggccgacggc cggtggtcgt acgggcggtc gaacatcgtc gcatcgcggg gatcgtagcg
      781 tgcgatcacc gacatcaccc tcgatacgtc ctccactgtc cgcgccatcg gcccgacctg
      841 ttcgagggag ctggcgtagg cgatgagacc gtagcgggag acccggccgt aggtgggttt
      901 gagccccacg acaccgcaga atgccgcagg acaccggatc gatccgccgg tatcgctgcc
      961 gagcgccatc gggacgaggc ccgcggcaac cgcggcggcg cttccgccgg acgaaccgcc
     1021 ggggaccctc gaggggtcga ccgggttttt ggtggggccg aacgcgctcg tctcggtcgt
     1081 cgagcccatg ccgaactcgt ccatgttggt cttgccgacg attgcggcac cctccctccg
     1141 gaggagttcg acgacgtagg cgtcgtaggg cggtatgtag ccctcaagga tccgggaccc
     1201 acaggtggtc tcgatacccg ccgtcgatat gttatccttg accgcgacgg cgatcccggc
     1261 gagcgcgccg ccggagaacg gcgctgtgct gcaggtggtg atgaaggcgt tgtaccggtc
     1321 gtccggtgaa aagtcgagag ttcccgccac tcacatcacc ctcggtgctt tgatgaaccc
     1381 gtcctcggtc gaccctgcgt tcgcgagggc cgcctcctgc gagaggcagg gccggggctc
     1441 gtcatctcta aaggcgttcg ttatcccgcc cgccggagcg ctctcgccct ctacggtgtc
     1501 gaggatctcg aagtattcga ggatcgcgtt gaactgcctg gtgaactccg gcacctcgtc
     1561 tcgtgatatg ccgatatccg caagctctgc tatatgctct atgtcgctct cagttaccat
     1621 gttctgccct gctgatctcc gttaagtacc cttgtaccga ctttttataa ccgttttgac
     1681 gggtgagctg gtgcatcgtc cgccatattc cgcttccgta ctgcatcgct ttcttctctg
     1741 cacgggctat ttcggcggga atgtgacgtt ctgcgacagt tcgcagggga tgctttctca
     1801 cccccccgcg caccttctcg cctgccggta tagcccgtgc ggcgcgcacc acccgggtat
     1861 cgatgtaggg catcgagaag tatgccccgt gcagcgccgc caccgcctga tcccgcgccc
     1921 tctggcgccc gtggtccatg acgtcccgct cgagttccgc cgcgaggtcg ggggatgaga
     1981 ggtagcgggc gtaaccgccg aagagctcgt ccgccccctg cccggcgagg atccgggtgt
     2041 ggccgttctc gccggcccat gcggcgacga aggagagggt cgccgcgatc gaggcgtcga
     2101 tggggttcgt cgggtcgggg atgacccgga cgacctcgcg gagagcctct gcgacctcct
     2161 cctccgtcgg cgtgacgatt gcgaggtcta gccccatcat ccgtgcggcc gcggcagccc
     2221 atagggcatc gtgcgacccg cggagcccca ccgccacgca ggggagaccg gcgagtccgg
     2281 cgatgagggc cgagtccacg ccgccggaga acgccaccgc cccttcgttg ctccggaggc
     2341 ctaccgcggt gacgatggcc tcctcgaggc cgcagggcgg cgggtcgggg gctatccggg
     2401 ccgcctcccg cccgccgcag cagatggtcc cgggcgcgat ctggccgggc atgataccga
     2461 aggtgtcgcg ggcgatgcag tcgtcccatt caagcaggaa ctcgccgccg caccgggcaa
     2521 gggccgccgg ctcctcgcgg agcatccgct ccacgtcggc cggcgagagc cgcacgccgt
     2581 cgcgttctat ccagcccttc aggttcatgg tcgctcgtct gtaatccggc aggtggttgc
     2641 catgaggctg ccgcagtacc cggcccctca aaaaagcgga tcagtatcaa tatttgatac
     2701 tgttaaacta taaatatacg caccgggttc ggcgcgaaaa acacggtatt tcagtgattt
     2761 ccgccgaata agcacccgta ttgggtgcag ggttgcggcc ccgatcctga atggaggggt
     2821 gccggcgccc gggaggaggc cacggaaggc actacgccca cgcgtcgccc cgtccggtcc
     2881 ggccctcccc tgacactttt gcgagatcta tgctgccaga actgctcacg ggcaggagtt
     2941 cacgccggct cctctgcggt ctccgcatcc tcgaaccgga cggggcgata ggacggagag
     3001 taagtgccct tgcacggggc ggcggagggg ggcgccggcc acctgtgaag agcgcccatc
     3061 acggtttctg tactcgacgc tctctctcct catgaagacc gcggcagatg cagaccagac
     3121 ctcgttgtgg tcatccggta tacgcgcact ggcccattcg cggcgagttg acgacaggga
     3181 gcgacatctc ctcatcgcac tcatgctcgc cgaagcagtc gtcgcaaagg aaggcgtcac
     3241 cgggagactc atcgagacac tcgaggcaga tcgtggttgc cggtttcccg cagacggaac
     3301 agcgaatgtc gggcggatcg tttcgggcaa cgagttcgat atctttcccg cctgccggag
     3361 catacgggac gagatcgagc accttgaagg tgagataggt ggagtcgccg tagtcataga
     3421 tgtatttgcc cgtcaggccc gccgagaaga gttctcccag ggggacattc atgcccttct
     3481 ctgtggtcca tctgtccggg taggaggagt attcctcgcc gtatatagtg aaggcggaga
     3541 ggtggctgca gcattcgagc cagacatacc tgagttcggt gtctatcgcc ttcagcgtgg
     3601 cattcttcct tgcaagaagg gtgatccagt atccgggttg gtactctccc tggaacatga
     3661 gaagccatga ttctgctccc gggtcggcct gccgctcctg tttgagttgc cgcatgacat
     3721 cgcccttagc tcttcgcttt gtggttgcga tattcccgat gagggtatgc ccgggatagg
     3781 tgcgttgcgc catgatgagt ctctggtata ttgagtgaaa attgttgcgt tgtacgatcc
     3841 cgtcggggct ggattaacct cgaggccatc gtttggctcg atgttttaaa tatagtgccc
     3901 tggcctgagg ttagcctgat tcggctggtt aattcagaaa gtggtagttg ttaccgcgga
     3961 aagcaggatc catctttctt gggaagatgt tcggcgtcag caccgatgca atgctgcaga
     4021 gcgttatagt cttcccgtct gccttcctga tcggtgcata tacggatctt tccgtctttg
     4081 tacaggcgtt gccgatcatg ctcctgatca ccgtcggcct gacgttttcc agcctggtga
     4141 agagtttcga ggggttcggc cttattcaga cgttcatcaa cctgccgatc ttcttcctga
     4201 acggggcgct ctttttgctg tcgaacgtgc cgcagtggct gttgtggatc aaccgctcta
     4261 acccgctcac gtacggcgtc gacgcactca gaaccgtcat gatggggggg cacgcgcccc
     4321 tgctcccgat gtacgtcaac atcggcgtac tgcgcgcctc cgatgcggtg atgttcacca
     4381 tcgggatata cgcgtttttg ggacggaata accatcaact actcctgacc ttggtcgggg
     4441 gctcctcgac ccatgatccc gaattcatcc cgccgacaag tgccgatgac ggtcgatctc
     4501 cgtggtcacc gcaacggccc cggcagaatg gagatctatt aattgcagaa aagtaatact
     4561 tgttgtatac attgtggtgg attgtattac tgaacagagg cgtttggggg tgcagggaac
     4621 ggcggatagg tacaaggggt atccggcatg ccggtgatct cctccgtggt aaacgccgtc
     4681 ctgctggtca tcgccggggt tatacttgcg aacatccttg ccgagaccgg gatcttttcc
     4741 cggctttcgt cgttcaccgg gccgctctgc cggttctccg gcctctccga tgcgtgtgtc
     4801 ctctcgatcg tcgcgatggg ggtaaacgcc acggcaggga agtcgatgct tgccgaatac
     4861 taccgtgacg ggaaggtgac ggagcgggag gtgatcccgg ttctcatcat gggtgcgttc
     4921 ccggtggtgc tcggggagtc gctcttccgc gtccagctcc ccacggccct ggtcctcctc
     4981 ggccccgtcg tcgggggggt ctacaccggg ctgaacctct tctcaagcgg catccaggcc
     5041 ctcttcgccc tcctctacag ccgccggttc ctcgcgggct ccgggcgcgc gccgcctccc
     5101 gtcccttccg cggccgctct tatgctcaat cgggaggtcg tcatttccgg gtgcaggaag
     5161 gcggtcccga cgctccggcg ggtcgttccc gtcaccctgg ccgcgctggt cgcgttctcc
     5221 ctcctctccg caacgggggt tatggacggc atcgccgccg cattcgatcc gctcctccgg
     5281 gccgtcggcc tccccggcga gagcgctgcg gcgctcgtgg cccagttcct gcacttctcg
     5341 gccgggtatg cgatcgttgc gtccctgctc gacgggggcg ttctcacgtt cgagacggcg
     5401 cttgtgacgc tcgtcatcgg gagcatggtg gtcatcacca tgatctacgt caagtactcc
     5461 gtgccgctct acctctcgct cttcggccgg ttcggcgtcc ggatttcgct cgccacgtat
     5521 gcagcgagca tggccgcgaa ggtgatcacg gtcgcgctgg tgtttcttct gttctgaagg
     5581 ggaacggaag tcagcatacc cccgggatac tgagaagtat ctcgggctgt tcggaagatg
     5641 ttcccggatc ggaaactttt tgtttctaca atggaaacat tttgttcacg gaatagaaac
     5701 tgcatgagag aggcgggatt aagcgaactg ttcaagaccg aggagcggat acgcatcctc
     5761 cggtacgtgg cagggcagcg gacggtaacg gcgacggcgg ttgtcgaagc gaccgggaca
     5821 tcaaaagctc tcgtctcacg gtacctgcac ctgcttgtca gggaagagtt ctgtacacgg
     5881 catggccgga tgtacatctg gcaggagaat gccagaagcc tggcaacaaa gcgtctgctc
     5941 aacatcgatc tcctccgggc gcaggtcccg ctccccgaat gggcccgggg gataggcgtc
     6001 tacgggagct atgcggaagg gacgaatacg gccgaaagcg acatcgatct ctgggtattc
     6061 gtcgacgaat acacaccgaa acttgagatt tgtgcagcga ggattgagaa aaccgtgagt
     6121 gtagcatcag ggactgaggt ccatatcctc atcctcaccc cggagaagct agcagaactt
     6181 cgggaggctg atacaccgtt ctatgccggc ctcatgaggt ggggtataac gatcgggggt
     6241 gcgtccattg gcaacgattg aggactgttt ctcgaaaaag ttgatccgaa gggtcgtgcc
     6301 ttccagagag aaggcgaacg agtcactgaa cctggcctcc tcctatcttt gcgaggcacg
     6361 gcaggtcgcc gggatcggtg cgaaccggat gagcctggcg ggagcgtaca tggcgtggtt
     6421 ccatgccgcc cgtgccgtac tcttccgtga cggtatccgt gagaagagcc attactgcat
     6481 cgagttgtat ctcgaaacgt atgtacgatc gggagacctc gaagatgagt gggtactgat
     6541 gttcggcaga attagggcac agcgccatga aagtcagtac tcattcgggc cgacgccgac
     6601 cgatgcggag gtctcggctg ccgttctgca tgctgagcga tttatcgaga ggatgaggca
     6661 gctgctctcc gaaacgccta aaaaagaatg ggtggggaga tgattcgatt attcatgcac
     6721 gagaggccgc tcacatcgga ctgagccggt cttacgaagc acctttgaat gcagtccgcg
     6781 acccctcgaa aagcagcgag gttgctcgtg ccctgggcat gtccaaatcc gcgattctcc
     6841 gtcggttgaa aaacctggaa gtaccgggac ttaacaggga ggagaggtcc ggcgcccata
     6901 ccaggtatcg cgcatcacca tgaacagcca cagcgcgttc taacttgcca ggggtgaagg
     6961 gggcgatgcg gttcgctctg ttcaacggat ctttgcaagc gtgtaactgc gccgggaaaa
     7021 gtgacggaac tcatcccggc gcagggagat ggccccgtaa accttaacat ggagatggtt
     7081 gaagggaaac agggccattt ctctgtttgt actcctgcat gattatcctg tcggaatggt
     7141 gtgctgcggg cgcatcgttc gccgcacgga gcttcgcgat cgagcggcgt acggcaggac
     7201 cccgcgcgct ggtctgtccc gggaaaaaat cggggaagcg ggttccctca attccctgtg
     7261 aggtcgggca tggcggtcgg cggtcgggta cggggcgacc ggtacgtctt cgagcatggc
     7321 cgcgagcggg tgtttcttct gctctgaaag gaaacgggcg tcgtcatacc ctgggatgct
     7381 gagacgctcc gggccgcccg gactattttc acgaaaccga cctgaggccg cccgtttttg
     7441 ggtgaaaaag agagggtggg accggtcatc tcttcttgag gagataccac cccgtcgcca
     7501 gaaccgcggc gacgaccagg actccggccg caatgagcgc ccacggggtc tcgcctcctg
     7561 tgggctgttc cgaaccgggc gggacatcac cgggtgcggt gccggccggg ccggtggtcg
     7621 cctccgccgg agatgcggcg gcccacgtca gggcgaagag gctgaagtgc gagacctctg
     7681 ccgtcacggt ccgggttgcc ggatccacgg ttgcgggaac ctcctgccat gcgccggtcc
     7741 cggggttgta ccacatcacc cgcagggtgg cggaattgcc gatcctcccc cactcctctt
     7801 cggagagcgt gtaggtgagg gtggccgggg ggtcgaatgt ggcgccggcc ggcccgcagt
     7861 cgagggcgaa cccgatcgtc gcccccggcg ggaccggggg caacccggcg gcgtcggcgc
     7921 gcgcgatggt cacttcgccg agtgggttac cgtccttgtc acgggcaacc gtccccttgc
     7981 cgacggtcag ggaaccggcg ccgtcggcgg tcccgaccgt cacggactcg ctcacctcac
     8041 ccgtctccga gagggggagg gacgcccgcc cgacaggcac cgtcggtgcg gacggggcgg
     8101 aggtcccaac aggggccggg gaggaccctg acggtccccc accgccgttg ccagacgggg
     8161 gcgtactccc tccggcaacg gagaggtcca ggcgggtcgt gctgtccggt gtgaaggtcg
     8221 ccgtttcccg cgccgtccgt ccgttgatca ggaaggtgat cgtctcccct gactgaccgt
     8281 cggtcctact gaccaccagg cggccccctt catgtcggct ggaactgccg tactccccga
     8341 tttttgtcgt cttgattgaa ccgcatgccg tatccccgat cagtgcggtg atctcggtcc
     8401 cggcgggtgc cggggaaccg tcgatcgtca cgccgcccca gaaatcgtgg gggaacgtgg
     8461 gaggtacatc ctctcctgcc gcgatcccga tcgtgagggc caggaggagg atcagagtcg
     8521 tcgcatgccg gtatctcatc gtcacgcccc gatggcagcg agggtatctg cattgttcat
     8581 gtagagccag tagccctgga agggagagag ttcccgcatc tccccgtgct tgccggtcgc
     8641 gccgcgaatg atcgagtcgt cgtaggcctg ttctctcgcc ttccagccga agaggtttgc
     8701 ccaggccgtc tcgaccgatc ggagcgtggt gaccgccggt tcggggacgg tatcggagaa
     8761 cccgatcgcg ttccagcccg catcgagata ttttgtgggc ggtggggagg tcgaagcggc
     8821 ggcgaaggac agtgggatct cataggtgcc gtttgagtag atccagatcc cgtcaagtgg
     8881 ccggaacgca tcctctcgac tcaggtcttc ccactggcag gttccgttgt agaacagcac
     8941 cgggcgcccg ttggtgtcca cgccgtcgaa gaccgcgatg gtgttgctgc cgtcggcaag
     9001 ccgcttcggg gtcgaaacga agttccagcc gggctggagg gtgatcgccg aagcatcaag
     9061 ggtgaagtga accgaggcga tctcgcccga ggtaacggtc acgatctcct ccacatcgag
     9121 gtagtcgtca atttcgagac ggatcgtgtg tttgccgacc gcgacgttca tcagcgtggc
     9181 attggtcact tcgccggtgt ctgtgccgtc gaggaagatc cgggcgccgt cgggtgtcga
     9241 ggtgacggcg atgctcccgg ccggttcggt caggaggaag ttgatcgtga tcgtctcgtt
     9301 atcagtgacc gtgacggtcc tcgaggcatc gatgtagccg gcgagtttca cggtgacggt
     9361 gtgctcgccg gccgcgacgt tctccaggag ggcgttcgtc agtttgccgg tgtcggcgcc
     9421 gtcaaggaag attgcggcgc cttcgggcat tgagacgacc gcgatgctgc cgggggcctc
     9481 gatgaggtca aggtggaggg tggtggtctc attctccgcg atggtgacct cggcgaccgc
     9541 gtcggcgtag ccggccttcc ggagagttat ggtgtgctca ccgacgccga tcccgtcaag
     9601 ggtagcattg gtgacctcgc cggtgtctgc gccgtcgatg aggatcgcgg cgccctcggg
     9661 cgtcgaggtg acctcaaggc tgccggtcag ggtggtcagt tcgaggtcga cggttacggt
     9721 ctcgtcgctg acgacggtga ccgacttcga agcgtcggcg tagccgccga gtttcagggt
     9781 gacgacgtgg tcgccggcca ggacgtcctc aagcgtgatg ggcgtgactt cgccggtgtc
     9841 ttcgtcgtcg agccagaccg ccgcgccggc gggggtcgag gtgacgagga tgctgcccgg
     9901 cagagccggg tactcgagga ccaggaaggc ggcggcggct tccatgtagt cggcgctgct
     9961 ctggaacccg accaggttct cgccggtgcg gaggagtgcg gtcacatcgc gctcgtcgac
    10021 gccgatctgg ctcgtccccg taaagctcca gacatccgcg aggcgttcgt cgttgatgag
    10081 gaggtcgccc ctggccggtc cggcgccggg ggcgacggtg atcagggcgg cgctcaggac
    10141 gtcatcggtc tcgacgccga agaacggcgc gtaggccgtc gcttgttcag gcgtggtccc
    10201 ctgggcctcg ccgccgtaga ggaggtcgaa gccttcgttc atgaagatgg tgtgcggggc
    10261 ggtggtgccg tcgtcgtaga cgaccgcaag cagcatcccg cggatcgaga cgttcccgcc
    10321 gccggggaag gtgttcgtga ggacggcggt gttgccggcg gcgctgaaag cgtcggtgac
    10381 gtcgtagacg gtcatgccgt agggatacga ggagccccac atcttctcat cctcgtagaa
    10441 ggcagaccga tcaacggtct cgctgttaaa ggccagggtg acgtttgagg ggaagatcgg
    10501 acccttgccc caggtgtagg ggacgtagag ccgggcgccg gcgatcgtgg cgccttcggg
    10561 gacggcaaga tcgccgcccg tccagttcac ggtgtaggtg gtccagttcg ggtaggtgct
    10621 ggcggagagg taggcgccgt cgccggccga ccagaggaag tcgccgcgga tgtcgtaggt
    10681 gacggcggtg gcgatgtccc cgccgtcggt ccagcgcatg ccgcggtagc cgttgtggac
    10741 gacggtcttc tcgatgctcg ccaggttgtt ctcttcattc gattcggcga tgaggccgtc
    10801 ggggtcggcg gtgacggtga tcgtcacgac gtcgccggcg ttgcggatcg tctcgtcggt
    10861 ccaggtgacg gtggtgcccg ccccggtcgc aagggcgtcg acggcgaccg tgccggccgc
    10921 gccgctgacg ttgaatttga cgtcgaacgc acctgcgtcg ccggtgccga tgttggtgat
    10981 cgttgccgtg taggtgttct cggacgcgga gaagacctcg ccgttgttcg acgagagggt
    11041 gctggccgcg agatcggggg cgtccccgcc ggcggggtcg ctgaccgtga tgcagccggt
    11101 cttgacctcg gaatcgctgc cggccgcgtt cgtgaccgtc aggttgaccg agtaagtgcc
    11161 tgcggtggtg taggtgtgga ccgggtgctg ctggtcgctg gtcgtgttgt ccccgaagtc
    11221 ccagagccag gaggtcggtg aaccgatcga ggtgtcgttg aactgcaccg tgagcggtgc
    11281 atcaccggcg gtcacgttcg ccgtgaagtt cgcgacaggt gccgagggca agggggcttc
    11341 cggatatttc acggcaagcg ttgcgagcgt ggtcttgtac gacccgccgt tcggcacgta
    11401 tgtga
//
LOCUS       DAOSII010000062        10966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_5891_length_10966_cov_44.742278, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000062 DAOSII010000000
VERSION     DAOSII010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10966
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5891_length_10966_cov_44.742278"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PLV90_06075"
     CDS             complement(<1..380)
                     /locus_tag="PLV90_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOF96719.1"
                     /translation="MKKTDRYSAHEEKRAPFGRILLCSLIACMLLLAIPVGAAEAVSF
                     PDTDLEAAVRDALDKPRGDITTDDMAGLGALDAEDKGISDLSGLEHAVNLRGLDLDSN
                     EISDLGPLAGLTDLQDLDLDSNEI"
     gene            957..1388
                     /locus_tag="PLV90_06080"
     CDS             957..1388
                     /locus_tag="PLV90_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOF96720.1"
                     /translation="MRDVHRVIEGRGNYTFIVHNHYTGDAQEVRVDPDRIALFEDKSS
                     IEGLPNACFFLRFDGEKAWCTVHLTRPALCREYCCRLLILDPQGRLAGRVTYQRALVP
                     DTDEFSRLWEQVRPALDDLSGVEWDDALIRILAPAGYRVRR"
     gene            complement(1450..2709)
                     /locus_tag="PLV90_06085"
     CDS             complement(1450..2709)
                     /locus_tag="PLV90_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96721.1"
                     /translation="MRLPYGDRFGRKKEAERPAIPPDEFSQQLACSRAEAEKILAAAE
                     GDLATLTGMRWSLEHTSLILGAAILVNLALLFAYTREYSLYWIMASFIFLMVNPFLLL
                     LPTDRGDLRSYVRYFIDLKDRERKSLEEIISWKSLEKGAAIPADAMEALRRLTHQRKS
                     LYELGWNLFFINCQPLAPGFLVLFALSSVFALTGWLVNGDFASYSAVIVVIQSAAIIV
                     FYGAIVYVQPYSRGFFAGMLGMQSRIRERYNEAWSQGLRYALTVAVVTTVAGVIFIAA
                     IVLPGVTYSSFASAEADIEFGAGMFALIFLTQLIVVRHLQGGYSRTLVHSLLSSRIEM
                     IREEVLPAIADLVPAPLCATATSGMAEDLKRITLDLARHRALKIDYVSLFGYFPVCMV
                     TPDIGAIMRVAGVVDGDRERTGTAGTA"
     gene            complement(2813..3292)
                     /locus_tag="PLV90_06090"
     CDS             complement(2813..3292)
                     /locus_tag="PLV90_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOF96722.1"
                     /translation="MQNGPAPDTSAPEDGSSVERGNYTSNETLVAFVESAAAYVRENG
                     KEKALAEFSDLNGSFIQGELYIYAYDFNGTTLAHPVNPEKIGVNRLNETEGDVGIFLR
                     KMNDAVRYGDGFGRIAYINPMRDRVLESKLAYGVQIDETWWLGSGVYTGSVDPDTSG"
     gene            complement(3475..3969)
                     /locus_tag="PLV90_06095"
     CDS             complement(3475..3969)
                     /locus_tag="PLV90_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96723.1"
                     /translation="MPHFDLFFKTEELRRRLEPHLHLIPPYFRFTVRVGTPEVRYFDP
                     KDPMWKGFPFPVPERTVYVFDDAIPARALGGGMDMRASVRVTRGDTDDEAIVLRIWHE
                     ILHAIGQPADDMVRRAAEWQSVSERLVWAAWQSLSRPVDVPFWHRKFYAWLTERAESG
                     AGGR"
     gene            complement(4440..4733)
                     /locus_tag="PLV90_06100"
     CDS             complement(4440..4733)
                     /locus_tag="PLV90_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96724.1"
                     /translation="MESTIRLNLTRVLEVTGELKHFLDLGAIRLQAAGQLSQEASEAL
                     IFAMADELEDHIRAMRDRQGTATIRDIRTWIRAWIDEQEAALGVKPPGNGDRG"
     gene            4975..5466
                     /locus_tag="PLV90_06105"
     CDS             4975..5466
                     /locus_tag="PLV90_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOF96725.1"
                     /translation="MEVKKVQERSGTVLERRTRTTGEEWHMSADDIMNKKVINSRGDD
                     IGSISDMRVSFPEGRVKYLVLKHGGTLGMGAKHFAIPPEAVIYRSGDDNFVVNIDKQQ
                     LDDRPGFDKDNWPQEADTSLIQSSGAITPPTREEAEAMRREVPPPAEVVKKETIETRP
                     RQK"
     gene            5642..6865
                     /locus_tag="PLV90_06110"
     CDS             5642..6865
                     /locus_tag="PLV90_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOF96726.1"
                     /translation="MDLRVWQVVFIAVIAIVFTVAYLSLYAQLNKTIWGEDSLLVVTP
                     WAIPAAVVFFSLLVGLSRKYLHAPDVIHEGFADSLKGGEGGKPDHRTFPGALLSSLFS
                     LLSGASIGPEGTITLLLMYVSSFVRKRLRIESPSAALGFDVAALASAFNGVIGSVLFT
                     GVFATEFQVGGNKNALRLLTWNLLAGTIGFGFYLLIGFPSFARSIPFEPMGEFLPVYI
                     LYAVALGIAGSLLAVFAGVSMQAAEKIMDRVFGGAVVTRVLAAGVVVAAVGYAIPELL
                     FSGEAQIHGIITDPARFGAVMLLGMAIAKILLLALSFKSGYIGGPLFPIIFSSTLVGL
                     ALSLVFPGIPVAILVLCIEAAALTFALGAPLTSILLVVVVGTADQNMIVLLTLSAVTA
                     MLIAARVKERMGGRA"
     gene            7015..7281
                     /locus_tag="PLV90_06115"
     CDS             7015..7281
                     /locus_tag="PLV90_06115"
                     /inference="COORDINATES: protein motif:HMM:NF023174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HOF96727.1"
                     /translation="METRAREEIPEGVTVIDEWVDSRGTQVFPVIETDDPVALMKLGF
                     PWSGFGYGDAPGCGVKRSVDYSRGMRRGAPYAPSRSCPGLTGTT"
     gene            complement(7236..8024)
                     /locus_tag="PLV90_06120"
     CDS             complement(7236..8024)
                     /locus_tag="PLV90_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF96728.1"
                     /translation="MALLSVEDLSFAYRDREILSRISFPVDTGEVLGLVGPNGCGKTT
                     LIRCVDGMLTPGSGRVLLHGRDLRGMHRRDIARAMAYVPQSAGNQTASTVFETVLMGR
                     RPYLNWTVSPADEEKVVEALAILDLGGLALRKIRELSGGERQRVMIARALVQETGVVL
                     LDEPTSNLDIRHQMAVMEVLSGLADERGLAVVIALHDLNLAATYCHRLLVMKAGEVYG
                     YGEPEEVLTPDMIERVYGIEAVVKQDLPAPYVVPVRPGQLREGA"
     gene            complement(8024..9076)
                     /locus_tag="PLV90_06125"
     CDS             complement(8024..9076)
                     /locus_tag="PLV90_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOF96729.1"
                     /translation="MEQSVVIQEGYTRLKKTRTLFIGGLIAGLVILIGVAVTLGSADY
                     TVAESYIAILAGLFPGIFSAPDLATTIVWDLRLHRVLFAVFAGFGLAVAGSVMQGILR
                     NPLASPFTLGIASAATTGASIAIVLGAGIAGSEYLVIANAFLFALLAALAIYGMARYR
                     GIGSETMVLAGIAIMYLFSAVTSLLQYVGSAEQLRAVVFWTFGSVDKSTWPKLGIVSL
                     VLACTIPYLLYRAWDLNALAEGDEVAAGLGVPVERSMIVFMMIASLITAVIICFTGTI
                     GFIGLVAPHITRMAIGGDFRTLLPASGLVGAVLLLAADSIARSVLAPQIIPVGIVTAF
                     LGVPFFIYLFMHRRDA"
     gene            complement(9088..10290)
                     /locus_tag="PLV90_06130"
     CDS             complement(9088..10290)
                     /locus_tag="PLV90_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOF96730.1"
                     /translation="MNRRRCLSALVLLLLVAVQAAAAEGIPGDADGDRVLTRAEYVAA
                     ALAYMIGDNDLARADIQDAAQVYAFWDGRPREITDSTGKTVTLYRPLRRVVIFAGESL
                     ETLRSLGFDMEKVVAVDKYSHAKNTFFPECREMGNVGSIWSPDMERVLILRPDTVFLY
                     ATISTAACDDIQNRLEASSPGIRVLRFDCFKPATYPDEIRTIASIIGKEERGEEFAAF
                     YESTMDRIRDGAAGIPDDERTRVYFEYWFDYTTVAASAGYNEKTEIAGGTNPFATGTA
                     EYPVVDPEAILVANPEVVIKLAGQGLAVGGYAGHDIAALDAIRSDMLGRPGWSGISAV
                     KDGRVYVIHSDILGGAQHFIGTAYLAKWFYPDRFADLDPHAIHQRYLTDFQGIDFDLA
                     SEGTFVYP"
     gene            complement(10287..10805)
                     /locus_tag="PLV90_06135"
     CDS             complement(10287..10805)
                     /locus_tag="PLV90_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96731.1"
                     /translation="MITLPANRLLCIALLLMVVPVAVSGAPVTRTLSTAEPGAGEAFT
                     VSLSIEDVALGGVVETLPAGYAFAETDHPADRILVRGQKIAFAVMNDTRIAYSVRAPA
                     SGSGDITGRWEDFIGEVDGAIPASHVAVDGIETAAESGGASPVAQEAASPFAVLAAIA
                     GILIAAGRGGRR"
BASE COUNT         2091 a         3434 c         3451 g         1990 t
ORIGIN      
        1 ctgatctcgt tcgaatcgag atcgagatcc tgcaggtccg tcaggccggc aaggggaccg
       61 aggtcgctga tctcgtttga atcaaggtcg aggccacgca ggttgaccgc atgctcaagt
      121 cccgatagat cgctgattcc tttatcctct gcatcgagag caccaagccc ggccatgtca
      181 tcggtcgtaa tatctccccg gggcttatcc agcgcatcgc gcaccgcagc ttcaaggtcg
      241 gtatccggga acgaaacagc ctccgccgcc ccgacaggta ttgccagaag cagcatacag
      301 gcgatcagac tgcacagaag gatgcggccg aacggcgccc gcttctcttc atgcgcacta
      361 taacgatctg ttttcttcat acgcatcacc atcccgggtc tttccggcat attcccgggc
      421 gaagggctcg gtggctacag ctgatcgcga tatccggaac catgctgcat aaccacatct
      481 cattcgcctg cacccccggg ccactcgcat cgagggagcg cacctgtatc cccgccatcc
      541 atatataagt gcctcatcgc gaacgtgaga accgttctcc gggcatgggg agcatccgcc
      601 cgatcaggct gttattcccg gcacgggatc gatcatcgcc ggctttcatg tccatccatg
      661 agttgccggc ctatggcggt gcatataccc ggtccttcga cacaaacgga gaaggcgccg
      721 ggcacccctc gttccgcgcc ggggtcgtgc ctccccccgg cctatcgcgg ttcccctgcg
      781 ccatgggtcg acataccggt ggccgagatc cggcgacctg tcaacgcccg ggcggacttc
      841 cggcagatat tatacgatta cggcgaagag taccggacga tgaagatgcc agtcgcccgg
      901 gggagagggt cgccatagcc ggtaagtgct gccagtgcgg caggtgctgc acccacatgc
      961 gggatgtcca cagggtcatc gaagggcgcg gcaactacac gttcatcgtg cacaaccact
     1021 ataccgggga tgcgcaagag gtgcgggtcg acccggacag gatagccctt tttgaggata
     1081 aaagcagtat cgaggggttg ccgaacgcat gcttcttcct gagatttgac ggcgaaaagg
     1141 catggtgcac ggtccacctg acacgccctg ccctctgccg cgagtactgt tgccgcctgc
     1201 tcatccttga tccgcaggga aggttagcgg gacgggtgac gtaccagaga gcgctcgttc
     1261 cggataccga tgaattcagt cggctctggg aacaggtgcg accggcgctg gacgacctct
     1321 ccggtgtgga atgggatgat gccctcatcc gtatcctcgc gccagccgga taccgcgttc
     1381 gccggtagta tgcccgggag aagaggatcg ccccggttct cctgtgccga tccggggcga
     1441 gatcggtgat tacgccgttc cggcggttcc ggtcctttcg cggtcgccgt ccactacccc
     1501 ggcaacacgc attatggcgc cgatgtccgg tgtcaccatg cagaccggga agtagccgaa
     1561 gagcgagacg tagtcgatct tgagcgcccg gtgccgggcc agatcaaggg ttatcctctt
     1621 gaggtcttct gccatcccgg aggtcgcggt ggcgcaaagc ggcgccggga caaggtccgc
     1681 gatggccggg agtacctctt cgcggatcat ctcgatcctg gacgacaaga gcgagtggac
     1741 gagcgtacga ctgtagcctc cctggagatg ccgcacgacg atcagctgcg tcagaaagat
     1801 aagggcgaac atgccggccc cgaactcgat atcggcttca gcagaggcga aactgctgta
     1861 cgtcaccccg gggagaacga tcgccgctat gaagatgacg ccggcaacgg tcgtcacgac
     1921 ggcaacggtg agcgcgtacc tgagcccctg gctccaggcc tcgttatacc gctccctgat
     1981 gcgggactgc ataccgagca tccccgcaaa gaaaccgcgt gaatacggct gcacatacac
     2041 gatcgcacca taaaagacga tgattgcggc ggattgaatg acgacgatca cggcggagta
     2101 cgacgcgaaa tcaccgttca caagccagcc cgtaagagcg aacaccgaag agagagcgaa
     2161 gagcacgaga aaacccgggg cgagtggctg gcagttgatg aagaagaggt tccagccgag
     2221 ttcatagagg gacttcctct ggtgcgtgag ccgcctgagt gcctccatcg catcggcagg
     2281 gatggcggcg cccttctcaa ggctcttcca tgagatgatc tcctcgaggc tttttctctc
     2341 gcgatctttg aggtcgataa aatacctgac ataagacctc agatcgcctc tatcggtcgg
     2401 gagcaggagg aggaaggggt tcaccatcag gaatatgaag gaggccatga tccagtagag
     2461 ggagtactcc ctggtatatg cgaacagaag cgccaggttg accagtatcg ccgctccaag
     2521 gatgagcgac gtgtgctcaa ggctccatcg catcccggta agggtcgcga gatcgccctc
     2581 tgccgcagcg aggatcttct ctgcctcagc ccgggagcag gcaagttgct gcgagaactc
     2641 atccggcggt atcgccggtc tctctgcctc tttcttcctc ccgaaccggt ccccgtacgg
     2701 cagcctcatc gatcattatt ttgcgggtta ttgtaaaaaa ggggttcctc accatataac
     2761 ccgcctctcc tcgacctcct ggtaaagggc atcgccgggc cgggcttccg cctcacccgg
     2821 atgtatccgg gtccaccgaa ccggtataga ccccggaacc aagccaccag gtctcgtcta
     2881 tctgcacccc gtacgccagt ttcgactcaa gcacccggtc acgcatgggg ttgatgtagg
     2941 caatccggcc gaaaccgtcc ccgtatctga cagcatcgtt catcttccga aggaagatcc
     3001 ccacatcccc ttcggtctcg ttcaggcggt tgacaccgat cttttccggg ttgaccggat
     3061 gggcgagggt ggtgccgttg aagtcatacg cgtagatgta gagttcgccc tggatgaacg
     3121 agccgtttag gtcgctgaat tcggcaagcg ccttctcctt cccattttcc ctgacataag
     3181 cggccgcact ctcgacgaag gcaacgagtg tctcatttga cgtgtagttc ccccgttcta
     3241 cagaactgcc gtcttccggc gccgatgtgt cgggagcagg cccgttctgc atacaaccgg
     3301 acgagatcaa agccaggccg agagcaagca tgacaacgag tgaaattgca agagaccgca
     3361 tactgtcaga ttgggtttgc cggtagatat gatttactgg caggcgtagc ctctgatagc
     3421 cacaggagcg acagcccgcc accggtttcg ccagaggatg ccgggcaccg tgccctatct
     3481 tccccctgca ccactctccg ctcgctccgt cagccaggcg tagaacttcc ggtgccagaa
     3541 cggcacatcc accggccggg agagcgactg ccaggccgcc cacacgagcc gttcggagac
     3601 actctgccac tcggccgccc gccggaccat atcatccgcc ggctgcccga tcgcgtgcag
     3661 gatctcgtgc cagatgcgga ggacgatcgc ctcgtcgtcg gtatctccgc gcgtgacccg
     3721 gacgctcgcc cgcatatcca taccgccccc gagcgcccgt gcgggtatcg catcgtcgaa
     3781 gacgtagacg gttctttccg ggaccgggaa ggggaacccc ttccacatcg gatccttagg
     3841 atcgaaatag cggacctccg gtgtccctac ccggacggtg aaccggaagt acggcgggat
     3901 gaggtgaagg tgcggctcaa gcctccgccg caactcttcg gtcttgaaga agaggtcgaa
     3961 gtgaggcatc ggggctgcat gggtggtggg tggtgataaa ttttcagatc gtgggtatcg
     4021 ttgccggcag gcccgttcgt cccgatgatg gtggatgcat catagaggag aagcgaagat
     4081 cgtgccggat ataaaggcat acaaaccgat acgaaacatc ctgccccggc cgttgaaccc
     4141 ccggtgagag gcttttctcc catgaatacc tgtatgccag accgccggat ccacgtgtgt
     4201 gcgcggctgc gcggcatcag acccggatac ggcagagaga acactcgcgc gacgatgcgg
     4261 ataggccagc cccatataat tgccgaaggg gaacaatgca cttcccgcgc cggtttgccg
     4321 aggattgacc gacacagcgc gaacaggaac cgaccgtggg tcgccgcgcg agtgttcttc
     4381 cggtgccggg gttgaaccct cctgcgcgga gccctaaacg cccttcaggt gcgaggcgct
     4441 cacccccggt ccccgtttcc cggaggcttt accccgagtg ccgcttcctg ctcgtctatc
     4501 caggcccgga tccaggtgcg gatgtcgcgg atggtagcgg ttccctgccg gtcgcgcatc
     4561 gcccggatat gatcctcaag ctcgtcggcc atcgcaaaga tcagcgcctc ggatgcttcc
     4621 tgtgataact gccccgccgc ctgcaggcgg atggcgccca gatcgaggaa gtgctttagc
     4681 tcgcctgtca cctccaggac cctggtgaga ttgagtctga tagtggactc catgaagagg
     4741 ggattggcgt atgcagtaca taactccatc tatttaaacc cgagccgcat cccggcctct
     4801 cctcccagaa tggcgcacat tatggcccat ttttccctga tggcgcatat cccgggctcc
     4861 cgggctgccg gttcaggaga gactcccggg ccatccggat gcaaaaccat tttatggacg
     4921 cccggcactc agtgcacccc gcttcaggag gcatgtctcc ctgaggtgga ggcaatggag
     4981 gtgaagaagg tgcaggaacg ttcaggaacg gttctggagc gaaggacgcg gacgacaggg
     5041 gaagaatggc acatgtcggc cgacgacatc atgaacaaga aggtgatcaa ctcccggggc
     5101 gacgatatcg gaagtatatc ggatatgcgg gtctcttttc ccgagggccg ggtgaagtac
     5161 ctggtgctca agcacggggg cacgctcggc atgggggcaa agcattttgc tatcccaccc
     5221 gaggccgtga tctaccgttc gggcgacgac aacttcgtcg tgaacatcga caagcagcag
     5281 ctggatgaca gaccgggctt tgataaggat aattggcctc aggaagcgga caccagcctg
     5341 atccagtcat cgggggccat aacacccccg acgcgggagg aggcggaggc catgcggcgg
     5401 gaggtgccac caccggcgga agttgtgaag aaagagacga tcgagacgcg gccccggcag
     5461 aagtaacccg gaacagcgcc tgttccgggc acaaaaactt tttttaaacc cggtcggcgg
     5521 cgtgatgccg ggataagcaa accacaagac agattagtca aaaaggcgag ctcattatgt
     5581 cgtgtctccc gtgccagcat ccgcagaagg cccggcgccg ccggaagtac cgggcagcac
     5641 cgtggatctc agggtgtggc aggtcgtctt catagccgta atcgccatcg tcttcacggt
     5701 agcgtacctg agcctttatg cacagctcaa caagaccatc tggggtgagg acagtctcct
     5761 ggtggtcacc ccctgggcaa tcccggccgc ggtggtcttc ttctcgctgc tcgtcggcct
     5821 ctcccgcaag tacctgcacg ctcccgacgt gatccacgag gggtttgcag actcgctcaa
     5881 gggcggggag ggagggaaac ccgatcatcg aacctttccc ggcgccctcc tttcatcgct
     5941 cttctcgctc ctctccgggg caagcatcgg cccggaaggg accatcaccc ttctcctcat
     6001 gtatgtctcc tcgtttgtcc ggaagagact caggatcgaa tcgccttcgg ccgcgctcgg
     6061 gttcgatgtc gccgcccttg cctcggcgtt caacggcgtc atcggcagcg tgctcttcac
     6121 cggggtcttc gccaccgagt ttcaggtcgg cggcaataag aacgccctcc ggctcctcac
     6181 ctggaacctc ctcgccggca ccatcggttt cggcttctac ctgctcatcg gcttcccatc
     6241 ctttgcccgg agcatcccgt tcgagccgat gggcgagttc ctgccagttt atatcctcta
     6301 cgcggtcgcc ctcggcatcg ccggatcgtt gctcgcggtc tttgcggggg tctccatgca
     6361 ggcggccgag aagatcatgg accgggtatt tggcggcgca gtcgtcaccc gcgttctcgc
     6421 cgccggggtg gtggttgcgg ccgtcggtta cgccatcccc gaactgctct tctccgggga
     6481 agcccagatc cacgggatca tcaccgaccc ggcccggttc ggggccgtca tgctccttgg
     6541 catggctatc gccaagattc tcctgcttgc cctctcgttc aagagcgggt acatcggcgg
     6601 cccgctcttt cccatcatct tctcatccac gcttgtaggg cttgccctga gcctcgtctt
     6661 cccgggcatc cccgtcgcga tcctggtcct ctgcatcgag gctgccgccc tcacctttgc
     6721 tctcggtgcg cccctgacct ccatcctcct cgtcgttgtc gtgggcacgg ccgaccagaa
     6781 catgatcgtg ctccttacgc tctccgccgt caccgccatg ctcatcgccg cccgggtgaa
     6841 ggagcggatg ggggggcggg cgtagcggac ggtccgccct tcaccgggct tttacacctc
     6901 aaaacccatg acgtcggccg tccatcccgg tatggcggga tgcggcaacg aaaggggaag
     6961 ggcatgcaat tcatgaacgt attcccccgg gagcccggga agacggccga gatcatggaa
     7021 acgcgggcca gagaggagat cccggaaggc gtgaccgtga tcgacgagtg ggtcgactcg
     7081 cggggaacac aagtctttcc ggtgattgag acggacgatc ccgtcgccct catgaagctc
     7141 ggcttcccct ggagtggctt tggctacgga gatgcacccg gttgtggagt caaaagaagc
     7201 gttgactatt cccgggggat gaggcggggg gcgccctacg ccccctcccg cagttgcccc
     7261 ggcctgaccg ggaccacgta gggcgccggg aggtcctgct tcaccaccgc ctcgatcccg
     7321 tagacgcgct cgatcatatc cggggtgagg acctcctccg gctccccgta gccgtagacc
     7381 tctccggcct tcatgacgag caggcggtgg cagtaggtgg ccgcaaggtt gaggtcgtgg
     7441 agggcgatga cgaccgcaag ccccctctca tcggcaagcc cggagaggac ttccatgacc
     7501 gccatctggt gacggatgtc caggttgctc gtcggctcgt cgaggaggac caccccggtc
     7561 tcctggacga gcgcccgggc gatcatcacc cgctggcgct ccccgccgga gagctcgcgg
     7621 atcttcctga gggcaagccc gccgaggtcg aggatggcga gggcctcgac caccttctcc
     7681 tcatcggcag ggctcaccgt ccagttcagg tagggccggc gccccatcag gaccgtctca
     7741 aagaccgtcg acgccgtctg gttcccggcc gactgcggga cgtaggccat tgcccgtgca
     7801 atatcgcggc ggtgcatacc ccggaggtcc cggccgtgaa gcagcactct gccgctcccc
     7861 ggggtgagca tgccgtcgac acatcggatg agcgtcgtct tcccgcaccc gttcggtccg
     7921 acaagcccga gaacctctcc ggtgtcgacc gggaacgata tacgggagag gatctcgcgg
     7981 tcgcggtagg caaacgagag atcctcgacc gagagcagtg ccatcaggca tccctccggt
     8041 gcatgaagag gtagatgaag aacggcaccc cgaggaacgc ggtcacgata ccgaccggta
     8101 tgatctgcgg tgcaaggacg ctccgtgcga tgctgtcggc ggcaaggagg aggacggcgc
     8161 cgacaaggcc ggatgcggga aggagggtgc ggaaatcccc gccgatggcc atccgggtga
     8221 tgtgcggggc cacgagcccg atgaacccga tcgtcccggt gaaacagatg atcaccgcgg
     8281 tgatcagaga ggcgatcatc atgaagacga tcatcgaccg ctccacgggc acaccgagcc
     8341 cggccgccac ctcgtcgccc tcggcgaggg catttaagtc ccaggcgcgg tagaggaggt
     8401 agggtatggt gcaggcgagg acgaggctca cgatcccgag tttcggccag gtggacttgt
     8461 cgaccgaacc gaacgtccag aagaccaccg cccggagctg ttcggcgctg ccgacgtact
     8521 ggaggagcga cgtcaccgcc gagaagaggt acatgatggc tatcccggca aggaccatcg
     8581 tctcggaacc gatcccccgg tagcgggcca tgccgtagat ggcgagcgcg gcaagcaggg
     8641 caaagaggaa ggcgtttgct atgacgaggt actcgctgcc cgcgatgccg gcgccgagga
     8701 cgatcgcgat cgaggcgccg gtggtggcgg ccgaggctat cccgagggtg aaggggcttg
     8761 caagcgggtt tcggaggatc ccctgcatga ccgatcccgc caccgcgagc ccgaacccgg
     8821 caaagactgc aaagagcacc cggtggaggc ggagatccca gacgatggtc gtcgcgagat
     8881 ccggggcgct gaagatcccg gggaagaggc cggcaaggat cgcgatgtac gactcggcca
     8941 cggtgtagtc ggcggacccg agcgtgaccg cgacgccgat gaggatgacg aggccggcga
     9001 tgagcccccc gatgaagagg gtccgggtct tcttcagccg ggtatacccc tcctggatga
     9061 cgacgctctg ttccatcgct gcaccggtca cgggtagacg aacgtcccct ccgacgcgag
     9121 gtcgaagtcg ataccctgga aatcggtgag gtatcgctgg tggatggcgt gcgggtcaag
     9181 gtcggcaaac cggtcgggat agaaccactt cgcgaggtag gcggtcccga tgaagtgctg
     9241 ggcgccaccg aggatgtcgg agtggatgac gtagacgcga ccgtccttca ccgcggagat
     9301 ccccgaccaa ccgggccgtc cgagcatatc cgatcggatc gcgtcgagcg ccgcgatgtc
     9361 gtgtccggca tacccaccga cggccaggcc ctgcccggcc agtttgatca cgacctccgg
     9421 gttggcgacg aggatcgcct ccgggtcgac gacgggatac tcggcggtcc cggtggcgaa
     9481 ggggtttgtc ccgccggcga tctccgtctt ctcattatat cccgcactgg ccgcaacggt
     9541 cgtgtagtca aaccagtact cgaagtagac ccgggtacgc tcgtcgtccg ggatgcccgc
     9601 agcgccatcc cggatccggt ccatcgtcga ctcgtagaat gccgcaaact cttcgccgcg
     9661 ctcttccttg ccgatgatcg aggcgattgt ccggatctcg tcagggtagg tggcgggctt
     9721 gaagcagtcg aaccggagga cacggatgcc ggggctgctc gcctcgagcc ggttctggat
     9781 gtcgtcgcag gctgcggtgc tgatcgtcgc gtagaggaag acggtgtccg gcctgaggat
     9841 gaggaccctc tccatgtcgg gggaccagat cgatccgacg ttccccatct cccggcattc
     9901 ggggaagaag gtgttcttcg cgtggctgta cttatcgacg gcgacgacct tctccatatc
     9961 gaacccaagc gagcgcagcg tctcgaggga ctcgccggca aagatcacca cccggcgcag
    10021 ggggcggtag agcgtcaccg tcttcccggt cgagtcggtg atctcgcggg gcctgccgtc
    10081 ccagaaggcg tagacctgcg cggcatcctg tatatcggcc cgggcgagat cgttgtcgcc
    10141 gatcatatag gcgagggctg ccgctacgta ctcggcgcgg gtgagcaccc ggtcgccgtc
    10201 ggcatccccc gggataccct ctgccgccgc ggcctggacg gcgacgagca ggaggagaac
    10261 gagggcagag aggcagcgcc gacggttcat cggcgaccac cccggcctgc cgcgatgagg
    10321 atgccggcga tcgccgcgag gaccgcaaac ggtgaagcgg cctcctgtgc gaccgggctt
    10381 gctccaccgc tctccgccgc cgtctcgatg ccgtccaccg cgacgtgcga tgcagggatg
    10441 gcaccgtcca cttcaccgat gaagtcctcc caccggccgg tgatatcccc gctcccggaa
    10501 gcgggggccc ggacggagta ggcgatccgg gtatcgttca tcaccgcgaa cgcgatcttc
    10561 tggccccgga cgaggatccg gtcggcaggg tggtcggtct cagcgaaggc gtagcctgcg
    10621 ggcagggtct cgaccacccc gccgagggcc acgtcctcga tcgagagcga gacagtgaag
    10681 gcctcccccg ccccgggctc ggcagtggag agcgtccggg tgaccggggc cccggatacg
    10741 gcgaccggga cgaccatgag cagcagggcg atacagagga ggcggttcgc cgggagagtt
    10801 atcatctgtt tcctgttgtc gattttgtaa tatgaatgtt tctgattgtg ctaataagtc
    10861 tgcagatcgg ctgttccgga gtatatcgaa gtgcagaccg gcgggccccg gggcgggatc
    10921 accccggtct cgccggtgag aggtggaacg agactggagt ttgggg
//
LOCUS       DAOSII010000063        10752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_6022_length_10752_cov_47.945312, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000063 DAOSII010000000
VERSION     DAOSII010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10752
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6022_length_10752_cov_47.945312"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="PLV90_06140"
     CDS             <1..567
                     /locus_tag="PLV90_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HOF96732.1"
                     /translation="FDAVPPGQGIENLMRRIEDDAESARSALERLYREKRPEARGNQE
                     PIWTIYGEENIRARLSDMFLGAERRVEMLVARDLLVEHVLALLGPVPESVQVDIITDT
                     WEGEQPSRFRIHAHPPGAHQKPHTPPGKGLRYESSGIFLVDDVRALVWMGSSGEQPSA
                     LYSESTGLVQFVRRYLTSIMEWVGATGQ"
     gene            complement(561..1307)
                     /locus_tag="PLV90_06145"
     CDS             complement(561..1307)
                     /locus_tag="PLV90_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF128 domain-containing protein"
                     /protein_id="HOF96733.1"
                     /translation="MHVPLKFTNHRIEEYALRVTYRPEEDTGTIIYNLSLIENRDLEF
                     ALSVMKEAHRTGITISDRIRVAEAGEEIGGYTVPDGRHAVCTMCSITLDALLLRRGIP
                     LNPIGGGVVEIEARVPRRFTSMILYRDTTLDPLEVLISQETTSILDVMHHGNGSILAN
                     IRECHMEAEPLVGTVLDELAAIGFSGVLDVGAPNAPLLGVPVSPQYVGVAMVGGTNAM
                     AAVREAGKPIVTRALKGVIDIREMGYLEDY"
     gene            1553..2518
                     /locus_tag="PLV90_06150"
     CDS             1553..2518
                     /locus_tag="PLV90_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96734.1"
                     /translation="MESGTREKARNVLKRILEAASFAVAEVDDPLVLSGIRGDTALLV
                     LCSDDPETIRKFDSMNYRLRTGDDDLVCRKLLFTLNPAVQTEDCMRWGADEFTLYAGR
                     AALAKVLGQTLVLDIGRPPPATRGESVEDAPAAVEEEPEAGPELPHLPVRITEKRACG
                     IAGLHGSAKCRFIPCWNYRYVSTGEREVKDRLVSFDAEGAGSINAINGMKMEIDQGSI
                     EREPVPFGSEIVPARLVREDVEGQIVREVIDRLTQRLRFRYEKGDAIFYEEKTLKPDR
                     NNIKVDLEIIYVPVWQVRGGSRIVEVNAFTGEVLSMPMDEGVELL"
     gene            2525..3850
                     /locus_tag="PLV90_06155"
     CDS             2525..3850
                     /locus_tag="PLV90_06155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOF96735.1"
                     /translation="MIVVIGGGPAGRIGAMHLAHAGREVRLIEKRKIGGQCLHDRCMT
                     ICALNDIARLIEYARTQKDLGILDSVPTVSYPAVVRRMRDVQEKLSTVLDVETRRAGV
                     DIVYGAEGRLEGDRVFVDDEEVRADAVLAATGSRAAIPDIPGTDLAGVYTYQTLPDME
                     ELPCRMVIIGGGVVAAEFAHVFRAFGSNVDIIARHEVLRGLDGKVRAAAIRDLSGVEI
                     REETAVLRIEGSGRVRSVLLAGGEEIEADAVLLATGLLPRSESLQGLKKGPDGAVIVD
                     RRMRTSVEGVYAAGDVIGPPYLTPAARREGTVAAENILGHPAIMDYTGIPQAMSLRYD
                     YAFATTGADGEGVTLSAPAPAGPGSFWDVAGGWAGLAQVRIDPETGRLIGAAACVPGA
                     ATLLSYVGYLMKRGITASDFDDIVEVHPSTDGVYGLARYAADMLRKRDG"
     gene            complement(3866..5488)
                     /locus_tag="PLV90_06160"
     CDS             complement(3866..5488)
                     /locus_tag="PLV90_06160"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HOF96736.1"
                     /translation="MGPHTIVIIGHRQPDTDSICSVIGYAELRNHAEPGKYVAARCGE
                     VNAETRFALETFNVEPPVYIASVEPSVSDIPLDTRSARQDVPTVDVAALMDTYDMRNI
                     PITDEEETLVGLVSEYGLARAYVRKNPREQLSLVPMPLATLARILDARTVVSAHEVLG
                     EGRVYTVIDALHVTLSRMTPDDIAIVGDNEPAQLALISAGVAALVIAEGAPVGERVIA
                     AARARGASVLATTLDAFSVGKMISLSLPARMIMETDVPTVRLEDSLEYAKGVVSSSRF
                     RTACVVGEDRRHIGLVSRTALMQDVQKSVILLDHNEYSQAVDGIEQADILEIIDHHRL
                     GAISTLKPVKFLNDPLGSTSTIVANKFIEASIEPTPPTAGLLLAGILSDTMVMKLSTT
                     TPVDIRAADYLAEIAGVDPAEFGPKLIQKGMDLDALPKDELLTRDMKRYNLFGRSIMV
                     SQVMTSSFEFAESEAGEIRTELDRLRTTMGVDCFFALFTNVFGDASDLFAAGDEACIA
                     KLGLRDQPVRLKGVMSRKKDFIPKFGQMFRQI"
     gene            5730..6404
                     /locus_tag="PLV90_06165"
     CDS             5730..6404
                     /locus_tag="PLV90_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF96737.1"
                     /translation="MSDEPLLRFEAVSKVYPLPAGDVVALDRVSLTVDAGEFIAVMGP
                     SGSGKSTLLHLMGCLDVPTGGKISLSGQDISGMSDDDLTGLRRDRIGFIFQQFNLIPL
                     LSAVENVEFPLTLTIGREESRRRAIEVMRAVHLDDALFTHRPAELSGGEQQRVAIARA
                     LVNNPDLLLCDEPTGNLDRKTGTAIMEILEAENREGKTVIVVTHDPEVATYARRTIRI
                     VDGKVV"
     gene            6401..6637
                     /locus_tag="PLV90_06170"
     CDS             6401..6637
                     /locus_tag="PLV90_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96738.1"
                     /translation="MIFLSFALRSPIAPPGHRDHRRRYGAFTFGEMFAAFNTTAIGEI
                     SSAVALGIGASLTDGLSPSWKAAQLNPIEVLRYG"
     gene            6884..8053
                     /locus_tag="PLV90_06175"
     CDS             6884..8053
                     /locus_tag="PLV90_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96739.1"
                     /translation="MITWLNKNFSSLQPTRAIVMRALRHLRPADRKKLFSEDIPEMRT
                     AEGRWFEAIVYEMILDLSLKTDLILAVVARGADGPSKVRRAQLGQNGLFYSNIGDIKV
                     RGNGQDLAEVDLMLVDHTGALTFGEIITSPADLKEFEAEIRYKKQLLGYLYGQPTVPF
                     LLISSVDISRTAVVRRLLKEPDNILLTTPTCEDLKSLIRPRDLKRSPPRQIRHEKLIS
                     IEKIAPRRPFDYKELHDERLQSIVAAVTSQKGIEKLGTPDEIPPIVKKVLFGGLYPSA
                     VRMLDARYPIQIKGKTYDPDAIQKQFSKVVLAVNIPEYRPIIYLRTRDKKEYLKMVPS
                     REGGFKFESRRTPHMAGFFLWLESIRTSLGAELTQELLDAFPAVHVSPAPAAGGS"
     gene            8112..8768
                     /locus_tag="PLV90_06180"
     CDS             8112..8768
                     /locus_tag="PLV90_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOF96740.1"
                     /translation="MDLIAELVSFLGFGLVAIASITAVMNPASTIAVYTALTEGMGGS
                     ERMKVIQTSMKIAFIVLAFFALTGQLIFTIFNITVPAFQIAGGILLVSVATGMLASKG
                     EAYSGADLENIAVVPLAFPLSCGPGTITTVILLASGPEGIWGIVAVFLGIIVALALSY
                     IGMLYGPRIFALIGEGGLRVVPKLMAIIVLAIAIQFIINGIAAAMPQLLANFDLAGLL
                     "
     gene            8765..9739
                     /locus_tag="PLV90_06185"
     CDS             8765..9739
                     /locus_tag="PLV90_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOF96741.1"
                     /translation="MTDIEIAFIQALQDQAAWLLILMQFFSFLGQPEFYLLIIPAFYW
                     CRDPRLGLRLGLLMGISGGLNEALKVVFHLPRPYWVSPDVRAFGSYPSFGLPSSHAQG
                     AVTFWGLIAAHVRRWWFWISVVAIILLIGISRIFLAVHFPADVIVGFAVGAGTLLGFL
                     ALEGPVGRRIARLPLSGQVFVALAGSLALALVSVSALATLGDWQVPASWAAGALERSG
                     QPIAPLSLRDAATASGLLFGFAAGAAVAWPKRGGMCAVGKWSVRLLRYIFGILVTGAI
                     WFGFGLLIPEDPGLTAFALLYIQAAAAGAWVSFGAPAAFAYLNLADRS"
     gene            9791..10252
                     /locus_tag="PLV90_06190"
     CDS             9791..10252
                     /locus_tag="PLV90_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOF96742.1"
                     /translation="MLVEGIALGWILIVLGAVLLLVEVTQPGFFIAVPATVLIILGVL
                     LLLGIDVLSSPAGLIIGVVAAIAAASITVWAYSRLTPERKPPTTLSRDSLVGLEGMVV
                     APVDAVTLSGKVRLGSTEWSARSGSGEIIPAGRRVIVIHSEGVHVVVKEVV"
     gene            10254..>10752
                     /locus_tag="PLV90_06195"
     CDS             10254..>10752
                     /locus_tag="PLV90_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOF96743.1"
                     /translation="MLPVGAIPWTGLVTIFLIIVIVIIFASGVVIVQPYEQGLQIRLG
                     QYIGRMNPGFRWVIPLITRVIKLDLRTEVMDVPRQEVITKDNSPTNVDAIVYVRVIDP
                     EKAYFEVMNYRAATVALAQTSLRGIIGDMELDEVLYNRDIINARLRDILDRETDAWGV
                     KVERVE"
BASE COUNT         1853 a         3359 c         3437 g         2103 t
ORIGIN      
        1 ttcgacgcgg ttccgccggg ccagggtatc gagaacctca tgcgccggat cgaggatgat
       61 gcggagagtg ctcgttcagc tctcgagagg ctctaccggg agaagcggcc ggaagcgcga
      121 gggaatcagg aaccgatctg gacgatctac ggggaagaga acatcagagc ccgcctctcc
      181 gatatgttcc ttggcgcgga gagaagagtg gagatgctcg tagcccggga tctcctcgtc
      241 gaacacgtac ttgcgctgct cggccccgtt cccgaatcgg tgcaggtcga tatcatcacc
      301 gatacctggg agggggagca gccttcccgg ttcaggatcc atgctcatcc ccccggcgcg
      361 caccagaaac cacacacgcc gccgggaaaa ggcctgcgct acgagagctc cggcatcttt
      421 ctcgtggacg acgtccgggc gctggtctgg atgggctcaa gcggcgaaca gccttcggcg
      481 ctctactcgg agtctaccgg actcgtccag ttcgtcaggc gctatctcac cagcatcatg
      541 gagtgggtgg gagcgaccgg tcagtaatcc tcgaggtagc ccatctcccg gatatcgatc
      601 acccctttga gtgcccgggt cacgatgggc tttcctgcct ccctgacggc ggccatggcg
      661 ttcgtgcccc cgaccatcgc cacgcccacg tactgcgggc tgaccggaac cccgagcagg
      721 ggggcgttcg gggcaccgac gtccaggacg ccggaaaacc cgatagccgc gagttcgtcg
      781 agcaccgtgc ccacgagcgg ttccgcttcc atatggcact cccggatgtt tgcaagtatg
      841 ctgccgttcc cgtgatgcat gacgtcgagg atcgacgtgg tttcctggga tatcaggacc
      901 tcaagggggt cgagcgtggt gtcgcgatag aggatcatgc tcgtgaaccg ccggggaacc
      961 cgcgcctcga tctcgacaac cccgcctccg atcgggttta acgggatgcc gcgccggagg
     1021 aggagggcat cgagggtgat gctgcacatc gtgcagacgg cgtgccggcc gtccggcacg
     1081 gtgtagccgc cgatctcttc ccctgcttcg gcgaccctga tccggtcgct gatggtgata
     1141 cccgtccggt gggcctcttt catgacggag agcgcgaact caaggtcgcg gttctcgatg
     1201 agcgagaggt tgtagatgat cgtcccggtg tcctcctccg ggcgataggt caccctgagc
     1261 gcgtactcct cgatgcggtg gttggtgaac ttcaggggga catgcattat cagatctctt
     1321 gtgcgatcag ggaaaagggt tcctttcggg gtgctccggc gggcggcggg gggagaaggt
     1381 ggatgaaccg gcatgtcgag cggttcagaa atgaggcggg caggccggag accgaccccg
     1441 gtcgcggggt gacgcgcgac tccgtggcag gctctccgga gagtaattcc ctccccctca
     1501 cccggccccg gatgcaaaac ttgttctacc tgtctgtgat attatacgat gaatggagag
     1561 tgggactcgg gagaaagccc ggaatgtgct gaagcggata ctcgaagcgg cctccttcgc
     1621 cgtggcggag gtcgacgacc cgctggtttt atccggaatc agaggcgata ccgccctgct
     1681 ggtgctctgc tctgatgacc cggagacaat cagaaagttt gattcgatga actaccgcct
     1741 gcgcaccggc gatgacgacc tggtctgcag gaaactgctc tttaccctca acccggcggt
     1801 gcagacggag gactgcatgc ggtggggtgc cgatgagttc accctctacg caggcagggc
     1861 tgcgcttgcg aaggtgctcg ggcagaccct ggtcctggat atcggccggc cgcccccggc
     1921 cacccgcggt gagtcggtag aggacgcccc cgcggccgtg gaggaagagc cggaagcggg
     1981 accggagctg ccccatcttc ccgtccggat caccgaaaag cgggcctgcg gcatcgccgg
     2041 gctgcacggt tcggcgaagt gccggttcat cccctgctgg aactaccgct acgtgagcac
     2101 cggcgaacgc gaggtgaaag atagactggt ctcctttgac gccgagggcg caggatccat
     2161 caacgccatc aacggcatga agatggagat cgaccagggg tctatcgaac gggaacccgt
     2221 ccctttcggc tccgagatcg tgcccgcccg gctcgtgcgg gaggacgtcg aggggcagat
     2281 cgtgcgggag gttatcgatc gcctcaccca gcggcttcgg tttcgttacg agaagggtga
     2341 cgctatcttc tacgaggaaa agaccctcaa acccgaccgg aacaacatta aggtcgacct
     2401 tgagatcata tacgtgccgg tctggcaggt gcggggcggg agcaggattg tcgaagtcaa
     2461 cgcgttcacg ggcgaggtcc tctcgatgcc gatggatgag ggcgtcgaac tcctgtaggg
     2521 aaccatgatt gtcgtcatcg gcggtgggcc cgcaggaagg atcggggcga tgcatcttgc
     2581 gcatgcggga agggaggtcc ggctcatcga gaagcgtaag atcggcggac aatgcctgca
     2641 cgaccggtgc atgaccatat gcgctctaaa cgacatcgcc cgactcatcg agtacgcccg
     2701 gacccagaag gatctcggta tactcgactc ggtcccgacc gtctcttacc cggcggtcgt
     2761 acggagaatg cgcgatgtcc aggagaaact ctccacggtc ctcgacgttg aaacccgccg
     2821 ggcgggagtc gatatcgtct acggcgccga gggcagactg gagggggacc gggtctttgt
     2881 cgatgacgaa gaggtccggg ccgacgcggt ccttgcggcc acagggtcgc gggcggcgat
     2941 ccccgatatc cccggcaccg atctcgccgg ggtctacacc taccagacgc tccccgatat
     3001 ggaagaactc ccgtgccgca tggtcatcat cggcggcggg gtggtggcgg cggagtttgc
     3061 ccacgtattc cgggcattcg gttccaatgt cgacatcatc gcccggcacg aggtgctccg
     3121 cggcctcgac ggaaaggtcc gcgccgccgc gatacgcgac ctctccgggg tagagatccg
     3181 cgaagagact gcggttctcc ggatcgaggg cagcggccgg gtccgatcgg tcctgctcgc
     3241 cggcggcgag gagatcgagg ccgacgccgt ccttctcgcc accgggcttc tcccccggtc
     3301 ggagtcgctg caggggctca aaaaaggtcc ggatggcgcg gtcatcgtcg accggcggat
     3361 gcgcacgagc gtggaggggg tctacgccgc cggcgacgtc atcggcccac cctacctcac
     3421 ccccgccgcc cgccgggagg gcacggtggc cgccgagaac atactcggtc acccagccat
     3481 catggattat acaggcattc cgcaggcgat gagcctccgt tacgactacg cgtttgccac
     3541 gaccggggcc gacggagagg gcgtcaccct ctctgccccg gcccccgccg gccccggttc
     3601 gttctgggac gtggcgggag gctgggcggg gcttgcgcag gtccggatcg acccggagac
     3661 cggtcggctc atcggggccg cggcctgcgt ccccggggct gcaaccctcc tctcctacgt
     3721 cggctacctg atgaagcgcg gcatcaccgc cagtgacttc gacgatatcg tcgaggtcca
     3781 cccatcgacc gacggcgtct acgggcttgc ccgctatgct gccgatatgc tccggaagag
     3841 agatgggtaa gatgtcccgg cgctcttaga tctgccggaa catctgcccg aacttcggta
     3901 tgaaatcttt ctttcgggac atgacgccct tgagccggac gggctggtcc cggagaccca
     3961 gtttggcgat gcaggcttca tccccggcgg cgaagaggtc gcttgcgtcg ccaaagacgt
     4021 tcgtgaagag ggcaaagaag cagtcgaccc ccatcgtcgt ccgcaggcgg tcgagttcgg
     4081 tgcggatctc gccagcctcg ctctctgcaa actcaaacga cgacgtcatc acctgcgaga
     4141 ccatgatgct ccgtccgaag aggttgtagc gcttcatgtc ccgggtgagg agctcgtcct
     4201 tcgggagggc atcaaggtcc atacccttct ggatcagttt cggcccgaac tcggccgggt
     4261 cgacaccggc gatctccgca aggtagtctg ccgcccggat atccacgggc gtcgtcgtcg
     4321 agagcttcat caccatcgta tccgagagga tccccgcaag gagaagcccg gcggtcggcg
     4381 gcgtgggctc gatagaggcc tcgatgaact tgttcgcgac gatcgtcgat gtcgatccca
     4441 ggggatcgtt gaggaacttc accggcttta aggtggatat cgcgccgagg cgatggtggt
     4501 cgatgatctc gaggatatcc gcctgctcta tcccgtccac ggcctgggag tactcgttgt
     4561 gatcgagcag gatcaccgac ttctggacgt cctgcatcag tgccgtccgg gatacgagcc
     4621 cgatatggcg ccggtcctcc ccgacgacgc aggcggtcct gaacctcgag ctcgagacga
     4681 cccctttcgc gtactcaagc gaatcttcca ggcgcaccgt ggggacatcg gtctccatga
     4741 tcatgcgcgc ggggagagag aggctgatca tcttgccgac gctgaaggca tcaagcgtcg
     4801 tcgcgaggac cgaggccccc cgcgcccggg cggcggcgat cacccgctca cccaccggcg
     4861 cgccctcggc gatgacgagt gcggcgactc ctgccgatat cagggcgagc tgggccggtt
     4921 cgttgtcccc gacgatcgcg atgtcgtccg gcgtcatccg cgagagagtg acgtgcaggg
     4981 catcgatgac ggtgtagacc cgcccctccc cgagcacctc gtgcgccgat acgaccgtcc
     5041 gggcatcgag gatgcgggca agggtcgcaa ggggcatcgg gacgagcgag agctgctcgc
     5101 gggggttctt tcggacgtag gcccgggcga ggccgtactc gctgacaagc ccgaccaggg
     5161 tctcctcttc atccgtgatc ggtatattcc gcatatcgta ggtatccatc agggcggcga
     5221 cgtcgactgt cggaacatcc tgccgggcgc tccgggtatc gagcggtata tccgatacgc
     5281 ttggttcgac gcttgcgata tagaccggag gctcgacgtt gaacgtctca agcgcaaacc
     5341 gggtctccgc gttcacctcc ccgcaccggg cggcgacata tttccctggt tcggcgtggt
     5401 tgcggagctc tgcgtagccg atgacgctgc agatgctgtc ggtgtcgggc tgcctgtggc
     5461 cgatgatgac gatcgtgtgt ggtcccatgc tctcctcgga cccccgcggg gcggtggttg
     5521 tctcaagagt tgtcgcgctc tgccgtcaaa ggctttgtgc cggatatccg gggaaacgtt
     5581 gaagtagtgg tttcctccac actccggcgt ggaaacccct caccccgggt ctgctgcaga
     5641 cccgccgatc ctggtgctct ctcttgaact ccgcctgccg ccgcggcctc cggagacgcg
     5701 acggtgatcg ccgtcaggag gccgcgacgg tgagcgatga gcccctcctc cggttcgagg
     5761 cggtcagcaa ggtctatccc ctcccggcag gcgatgtcgt ggcgctcgat cgcgtctccc
     5821 tcaccgttga tgcgggtgag ttcatcgccg tgatggggcc ctcgggctcc ggaaaatcga
     5881 cgctcttgca cctgatgggc tgccttgacg tcccgaccgg cgggaagatc tctctctccg
     5941 ggcaggacat atcggggatg agcgacgacg acctcacagg gctccggcgc gaccgtatcg
     6001 ggtttatctt ccagcagttc aacctcatac cgcttctctc ggcggtcgag aacgtcgagt
     6061 ttcccctcac cctcaccatc ggccgggagg agagccggcg ccgggcgatc gaggtcatgc
     6121 gggcggtgca tctcgacgac gccctcttta cccacaggcc ggccgagctc tccggcggcg
     6181 agcagcagcg ggtcgcgatc gctcgtgcgc tcgtcaacaa ccccgatctt ctcctctgcg
     6241 acgaaccgac cggaaacctg gataggaaga ccgggaccgc gatcatggag atcctcgagg
     6301 cagagaaccg ggagggcaag acggtcatcg ttgtcaccca cgacccggaa gtcgctacat
     6361 acgcccgccg cacgatccgg atcgtggacg ggaaagtggt atgatcttcc tctccttcgc
     6421 tcttcggagc ccgatcgctc cacccggcca tcgagatcat cgtcgccgtt acggggcctt
     6481 cacgttcggc gagatgttcg ccgccttcaa cacgactgcc attggcgaga tctcctccgc
     6541 ggtggcgctc ggtatcggag cgagtctcac cgacgggctc tctccctcgt ggaaagcggc
     6601 gcagttgaac cccatcgagg tgctccgcta cggatgacgg gcctggaggc tatcccccgg
     6661 cgccgccgtc tgccctgtgc cgggtcggtt ctcccctccc ggtgtccgcc gcgatgccgg
     6721 gcgaagatcc cggtggggtg agaccatgcg taatttcata gtcatggaga ccggaaactc
     6781 tatgagcact tataggatga taacctccct atcaggaagt gctccgaccc atccggtccc
     6841 caatacgata ccaatacccg gctgtgtgga acgaatgtta cagatgatta cgtggcttaa
     6901 caagaacttc agctcgctcc agccgacgcg agcgatcgtc atgagggcac tgcgccacct
     6961 gcgccccgcg gatcgaaaga aactcttctc cgaggatata cccgagatgc ggaccgcgga
     7021 agggcggtgg tttgaagcga tcgtctacga gatgatcctc gatctctcgc tgaagaccga
     7081 cctcatcctc gctgtcgtgg cccggggcgc cgatggccca tcgaaggtcc ggcgtgcgca
     7141 gcttggccag aacggcctct tttactcgaa tatcggtgac atcaaggtcc ggggcaacgg
     7201 gcaggatctc gccgaggtcg acctgatgct cgtcgaccac acgggggcgc tgacgtttgg
     7261 cgagatcatc acgtctcctg cggatttaaa agaatttgaa gcggagatcc gttacaaaaa
     7321 gcagctcctg ggctatctct acgggcagcc gaccgtgccg ttcctcctca tctcttcagt
     7381 cgacatatcg cggacggccg tcgtccgccg cctcctcaaa gaacccgaca acatcctcct
     7441 caccaccccg acatgcgagg acctgaaatc cctgatccgc ccaagggacc tgaagcggag
     7501 cccgcccagg cagatccggc acgagaagct gatatcgata gagaagatcg ccccgcggcg
     7561 gccgttcgac tacaaggaac tgcacgacga gcggttgcag agcatcgtcg ctgccgtgac
     7621 ctcccaaaaa ggcatcgaaa aactcggcac accggatgag atcccgccga tcgtgaagaa
     7681 agtcctcttc ggcgggctct acccttccgc cgtccggatg ctcgacgccc ggtatcccat
     7741 ccagatcaag gggaagactt acgatccgga tgcgatccag aagcagttct caaaggtcgt
     7801 cctcgcggtg aatatccccg agtaccgacc gatcatctac ctccggaccc gggacaagaa
     7861 ggagtacctc aagatggtgc ccagccgtga gggggggttc aagttcgaga gccggcggac
     7921 gcctcacatg gccgggttct tcctctggct cgagtccatc cggacatcgc ttggggcgga
     7981 gctgacgcag gaactcctcg atgcgttccc tgcggtccac gtttctccgg ccccggcggc
     8041 cggaggatcg taaggtacct atggggccgg ggcgcatgta tctctatgtt tatagggtgt
     8101 ggtgatccga tatggacctg atcgctgaac ttgtcagttt tttagggttt ggcctggttg
     8161 cgatagcgtc gatcaccgcg gtgatgaatc cggcctccac gatcgccgtc tacacggcgc
     8221 tcaccgaggg gatgggcggg agcgagcgca tgaaggtcat ccagacatcc atgaagatag
     8281 ccttcatcgt gcttgccttc ttcgccctca cgggccagct gatcttcacg atcttcaaca
     8341 tcaccgtccc ggcgttccag atcgccggcg gtatcctcct ggtcagcgtg gcgaccggaa
     8401 tgctcgcctc gaagggcgag gcctactcgg gtgcggacct ggagaacatc gccgtcgtcc
     8461 cgctggcatt cccgctctcg tgcggcccgg ggacgatcac gacggtgatc ctgcttgcgt
     8521 cgggaccgga aggcatctgg ggtatcgtcg ccgtcttcct cggtatcatc gttgctctcg
     8581 ccctctccta catcgggatg ctctatgggc cgagaatctt cgccctcatc ggggagggag
     8641 ggctccgcgt cgttccgaaa ctgatggcga tcatcgtcct tgctatagcc atccagttca
     8701 tcatcaacgg tatcgctgcg gcgatgcccc agctactggc aaactttgat ctcgccgggc
     8761 ttctatgacc gatatcgaga tcgcattcat acaggctctc caggatcagg ccgcatggct
     8821 cctgatcctc atgcagtttt tttcattcct cgggcaaccg gagttctatc tgctcatcat
     8881 cccggcgttt tactggtgcc gggatccgcg cctcggtctc cgcctcggcc ttctcatggg
     8941 tatatcgggc gggctcaatg aggctctcaa ggttgtgttt catcttcccc gcccctactg
     9001 ggtcagcccg gatgtccggg cgttcgggag ttatccatcg ttcgggctgc cgtcatcgca
     9061 tgcccaggga gcggtgacgt tctgggggct gatcgccgcc catgtccggc ggtggtggtt
     9121 ctggatatct gtggttgcga ttatactcct catcggcata tcccgcatat ttcttgcggt
     9181 gcatttcccg gctgacgtta tcgtggggtt tgccgtcggc gccggcaccc ttcttgggtt
     9241 cctcgccctc gaagggccgg ttggccgccg gatcgctcgc ctccctcttt cagggcaggt
     9301 gttcgtcgcg cttgccggat ccctcgctct ggcgctggtc tccgtatccg ctctcgcgac
     9361 cctcggggac tggcaggtcc cggcatcctg ggcggcgggg gcgctcgagc ggtcggggca
     9421 gccgatcgct cccctctccc tccgggatgc cgccacggcg tcggggcttt tgttcgggtt
     9481 tgcggccggt gcggcggttg cgtggccgaa acgggggggc atgtgcgccg tcgggaagtg
     9541 gtctgtccgc ctgctccgtt atatcttcgg tatactggtg acgggggcga tctggttcgg
     9601 gttcgggctt ctcataccgg aagacccggg cctcaccgcc ttcgcccttc tgtatatcca
     9661 ggctgccgcc gccggtgcct gggtctcgtt tggggctccg gcggcatttg cctatttgaa
     9721 cctggcggat cgctcctgac ggacccccct tcccataccc tttttatccc gccgggtcca
     9781 tctctctcgt atgcttgtag aggggatcgc tcttggctgg atactgatcg tgctcggggc
     9841 ggtgctgctc ctggtcgagg tcacccagcc cgggttcttc atcgctgtcc ctgcgacggt
     9901 cctgatcatc ctcggcgtcc tccttctcct cggcatcgac gtcctcagtt ctcctgccgg
     9961 tctcatcatc ggggttgtgg cggcgatcgc tgcagcctcg atcaccgtct gggcgtatag
    10021 tcgcctcacg cccgagagga aaccgccgac gaccctctca cgggattcgc tcgtcggcct
    10081 cgaggggatg gtggtcgcgc cggtcgatgc ggtgacgctt tcggggaagg tgcggctcgg
    10141 cagtacggaa tggagcgcgc gttccggatc gggggagatc ataccggccg ggagaagggt
    10201 tatcgtcatc cactccgagg gcgtgcatgt tgttgtaaaa gaggttgtgt aaaatgcttc
    10261 cggtaggtgc gattccgtgg acgggattgg tcacgatctt cctcatcatc gtgatcgtca
    10321 tcatcttcgc cagcggcgtg gtgatcgtgc agccatacga acaggggctc cagatacgcc
    10381 tcggacagta tatcgggagg atgaatcccg ggttccggtg ggttatcccg ctgatcacga
    10441 gggtcataaa gctcgatctc cggacggagg tgatggatgt cccgcggcag gaggtgatca
    10501 cgaaggacaa ctcgccgacg aacgtggatg cgatcgtcta cgtccgggtg atcgaccccg
    10561 agaaggccta cttcgaggtg atgaactacc gggcggcgac ggtggcgctc gcgcagacga
    10621 gtctccgggg tatcatcggc gacatggagc ttgacgaggt tctctacaac cgcgatatca
    10681 tcaacgcccg tctccgggat atcctggata gagagaccga tgcatggggc gtgaaggtcg
    10741 agcgggtgga ga
//
LOCUS       DAOSII010000064        10469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_6218_length_10469_cov_46.349722, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000064 DAOSII010000000
VERSION     DAOSII010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10469
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6218_length_10469_cov_46.349722"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1064)
                     /locus_tag="PLV90_06200"
     CDS             complement(3..1064)
                     /locus_tag="PLV90_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOF96744.1"
                     /translation="MNSSPSPSNSSRFEGCTLTGALSVLTEIRDAVCVIHGPSGCAHH
                     NFSLLHATLLSNDRLEVPRLLSTRLTEKDIIFGGEEALESSIARALSLSPAPSSVFVL
                     STCIVETIGDDTAAVSAKAWGVPVIAVPTAGFLGGVFETGVRNALSSAALLAESARER
                     TLSANLIGEKNLEYGVDENAAEIVRLLARLGIGINLRFVRGIATGDIGRLGSAAVNIL
                     REPALRPVGEDLKRRFSTPFVDSFPVGLAGTCRFLADIGRICGVDASAAIEEEQAFQR
                     EMLSRFADLEGSRVCFRSPHSMLEGDPAAEAVLAECAIALDLAVDPDGTPIPLPYPAP
                     VGTAGVRRMLNRWRVQIRG"
     gene            complement(1024..1842)
                     /gene="cfbC"
                     /locus_tag="PLV90_06205"
     CDS             complement(1024..1842)
                     /gene="cfbC"
                     /locus_tag="PLV90_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide reductive
                     cyclase ATP-dependent reductase subunit"
                     /protein_id="HOF96745.1"
                     /translation="MKQIALYGKGGIGKSTTAANLSAALAGEGLDILQIGCDPKHDST
                     RMLMHGTWIPTVLDLIRERGDEGITVDDVVYRGFRGVRCVEAGGPEPGIGCAGRGIIA
                     TFQLLERLDALKGDVIVYDVLGDVVCGGFAMPMREGYAQEIYLVTSGELMSIYAANNI
                     AKAIARLSRRVRSRCSLGGVICNAKNIEGERDLVEDFARRINSRLIAYVPRDRVVQVA
                     ELHRQTVVEYAPDSSQAAVYEELGRAIYANRKTSIPTPLEMDELESFAFEFVQV"
     gene            2073..2693
                     /locus_tag="PLV90_06210"
     CDS             2073..2693
                     /locus_tag="PLV90_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96746.1"
                     /translation="MDPRFRSRLIAAIVLLIVVCSAFSVSPVAGFRLENRDGSGTDAT
                     LAEALVLQQSTRIREEFIKDITVYIDSRNRVFAQQGTYGNTSGLYVPAEDAIYIRSDR
                     HPAQADEAFARQVGYRVYHTMRFSESTVFPALDAGSGPCMARLSIPPGEEREAALFAE
                     AFMLYHASPALLKEDAPATYAYMDLLVKSGGDCAAVDGLYTHGRPA"
     gene            complement(2849..4306)
                     /locus_tag="PLV90_06215"
     CDS             complement(2849..4306)
                     /locus_tag="PLV90_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOF96747.1"
                     /translation="MDRIEQFSTIAPDIGNIFRYISIGTAVPLIVAVIYREWDMILPM
                     LSVPITLFLLGTLLVRTPRGEREAPLSAALMAVALIWLVMALASALPFSLGLGVSYLD
                     AVFEAMSGWTDTGLTVMRSVDDLPRTLLFWRSLMQWLGGIGIVAFTIAMASRTGLTQF
                     RLYRSEGRSEALMPSVVATGMEMWKIYLILTAASIGLILLSGVPLWDAVNIALTAIST
                     GGFSVHSAGIPYYHNPLLEVFIVPVMIAGALPFKLYYLLYRQRGIRVLDDQQARLLFM
                     LVALGIVVITWDLVTLSATGLTTAVHSALFMSAAAITSTGFQIVSPNEWASVTVLFLS
                     MLMVIGGASGSTAGGIKLSRVVLGAQSLIWWFRRMFVSGKVLVPFKYDGRVIPKNIAD
                     LEVSRNMLIIMLYFLTIFVATVLVMHLQPTAFDSSNVIFEVVSAMCNCGISAGFVSPD
                     MADSAKVLFILVMWIGRLEIIPVIMLVMGILKGFD"
     gene            complement(4388..5407)
                     /locus_tag="PLV90_06220"
     CDS             complement(4388..5407)
                     /locus_tag="PLV90_06220"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HOF96748.1"
                     /translation="MPPSDFKRVIAESPYVFVIGVAGDSGSGKTTFTRAIRAIFGRDL
                     VSMITIDDYHRYDREERKALGITPLVPEANRFDLLEEHLRDLKAGKTISKPVYNHDNG
                     KFDPPVPFSPTKILIVEGLHPFITRTLRDLVDFKLYVDPDPDVKRAWKIKRDVEQRGY
                     SPEAVIKEMEERKPDYEQYVAPQCRFADAVIRIAFSKYGRKVSEERNVYHVSLCQNGI
                     DRSIHNVDLSIDLYPILSLSQRSFMVEFTSEDVSGRPMGALTFDGELNYAVARKLEQN
                     IEIQTQVQPIDLVKNGAYLTAGGMAQLILAWRIINRRIAIEGAPGAAKEEEPAGYDGC
                     WCGRT"
     gene            complement(5730..6920)
                     /locus_tag="PLV90_06225"
     CDS             complement(5730..6920)
                     /locus_tag="PLV90_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOF96749.1"
                     /translation="MAAREIVPGVRSVGVIDWDRRIFDELIPLPDGTSYNSYLIRGSE
                     KTALIDTVDPVKTAELMANLDRAGAETIDYIIANHAEQDHSGSIPAMLARFPEAKVVT
                     NQKCRDFLVDLLHIPVDRTIVVGDKETLPLGGKTLEFILAPWVHWPETMLTYLQEDRI
                     LFSCDFFGSHYATSSLYVPNEGVIYESAKRYYAEIMMPFRSSIKGHLAKLAARDIAMI
                     APSHGPIHKRPAFIMDAYRDWTDDAVKNMVVLPYVSMHGSTQAMVDHLVDALMQRGVP
                     VQPFNLPKTDIGDLAEALVDAATVVIGAPTVIFGPHPQAVYAAYLANLLRPKTRYATV
                     IGSYGWGGKTVDTVKNMLDRLKVELLEPVYIKGYPKEADFAALDRLADAIKKKHEEAG
                     IIVS"
     gene            complement(7149..7877)
                     /gene="nfi"
                     /locus_tag="PLV90_06230"
     CDS             complement(7149..7877)
                     /gene="nfi"
                     /locus_tag="PLV90_06230"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866233.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HOF96750.1"
                     /translation="MEIRETHPFDLTPAEAAHLQEALRTRVRLTGDIGEVSLVAGVDA
                     SYVKGSNVIHAVVVALGYPDLAVVERVAARTETSFPYIPGLLTFREGPAVLKAFRKLR
                     SEPDVIFFDGQGIAHPRGLGIASHIGVLLDRPTVGVAKSLLVGSVEEPGVTRGSTAPI
                     IRDGETIGMAVRTKERTKPVYVSVGNRIDLFQAVDLVLATARRYRLPEPTRQAHLFAN
                     LARKTGIGEDRQTGLPSPEARREG"
     gene            8080..8322
                     /locus_tag="PLV90_06235"
     CDS             8080..8322
                     /locus_tag="PLV90_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOF96751.1"
                     /translation="MELGYGPEFHLQDVNGMARWVCSVCDYEYNEEAGDPAAGIPPGT
                     RFEDLPADWRCPECMVGKDAFVRVDEEVEKNGENDL"
     gene            8303..9934
                     /locus_tag="PLV90_06240"
     CDS             8303..9934
                     /locus_tag="PLV90_06240"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOF96752.1"
                     /translation="MVKTTSKGPETTVADVLIAELAAWGVNLFFGIPGASSLPLVDAV
                     RRNQDVRYIVVRHEQTAAMAASAYNKFTGKVAACLTIAGPGATNLATGLYDAKEDHAS
                     VLSLNGQVKMQYSGPGGIQEIDQDAFFRPITVFNSTVADPMTAVKLLTRALRYAIIAR
                     GVAQLSVPNDIQAAPLKAAYCERETCLPSTCVAPTDEAVRAVVEAIDGAHRPVIVAGF
                     GALHAAGAVLDLATKIQAPIVTTLRGKGILPDDNEWVAGVHGELGTPHALMLVLKSDL
                     VIACGMSFSDHTGIPPGKRAVQIDIDPLQLGKHPLAVGVYGDCALALPRIAGQVRARE
                     DPDTRQWLAEKKREWLDRLDQEADPEATPIRPPYVMKVLSDLLPEDAIISIDVGDNQW
                     WFGRNFRMKRQRFAMSGYLGTMGFGLPGAIAAKLAYPDATVVCIVGDGGFSMVMGDLV
                     TTIKYDLPIVVVVLSNRELAMIREEQREEHYPPFGIDLTNPDFAAYAEACGGAGIRVT
                     RPQDLPGAVREALKMNTSVVIDVETDPERFPQKKK"
     gene            complement(9942..>10469)
                     /locus_tag="PLV90_06245"
     CDS             complement(9942..>10469)
                     /locus_tag="PLV90_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HOF96753.1"
                     /translation="FGRVSGCHINPAVTIALFATGRFPGRDVGPYIAAQLLGAAAASL
                     LFAWAIGPDAVTIGGLGATAPFHGIGYLQAIAIEAIGTFLLMLVIMGAAVDERATPGF
                     AGLVVGLVVAGIITTTGNLTGASLNPARTFGPYLGDWLLAGQNLWEFFPIYIVGPIIG
                     AVAAAILYDNLCCGE"
BASE COUNT         1917 a         3274 c         3365 g         1913 t
ORIGIN      
        1 cttcaccccc ggatctgcac ccgccaccgg ttgagcatcc ggcggacacc ggcggtcccc
       61 acgggcgccg gatacgggag cgggataggg gttccatccg gatcgacggc aaggtcgagg
      121 gctatggcac actcggccag gaccgcctcc gcggccgggt ctccctcgag catggagtgg
      181 ggggaccgga aacagacccg gctcccctcg agatccgcga accgcgagag catctcgcgc
      241 tggaaggcct gttcctcctc gatggctgcc gaggcgtcca cgccgcatat tcgcccgata
      301 tccgcaagga accggcacgt cccggcaagc ccgaccggga acgagtccac gaagggcgtg
      361 ctaaaccgcc tcttgaggtc ctcgccgacc ggccggagcg ccggctcgcg gagaatattg
      421 acggcggcag aaccgaggcg cccgatatct ccggtcgcga tcccccgcac gaaccggagg
      481 ttgatcccga taccgagccg ggcaaggagg cgtacgatct ccgcggcgtt ctcgtcgacg
      541 ccgtactcca ggttcttctc gccgataagg ttcgccgaga gggttctctc cctcgcggac
      601 tctgcgagca gggcggcgga agagagagcg tttctgacgc cggtctcaaa gaccccgccg
      661 aggaaccctg cggtcggcac ggcgatcacc ggaacccccc atgcctttgc agagaccgcc
      721 gcggtgtcgt cccctatcgt ctcgacgatg caggtcgaga ggacgaagac ggaagagggg
      781 gccggggaga gcgagagtgc ccgcgctatg ctgctctcca gggcctcttc cccgccgaat
      841 atgatgtcct tctccgtaag gcgggtggag aggaggcggg ggacttcgag gcggtcgttt
      901 gagagcagcg tggcgtgaag gagggagaag ttgtggtgcg cacatcccga cgggccgtgg
      961 atgacgcaga cggcgtcccg tatctccgtg aggaccgaga gcgctccggt cagggtgcac
     1021 ccctcaaacc tggacgaatt cgaaggcgaa ggactcgagt tcatccatct cgaggggggt
     1081 gggaatgctc gtcttccggt tcgcatagat cgcgcgcccg agttcctcgt agaccgccgc
     1141 ctggctggaa tcgggcgcat actcgacgac cgtctgccgg tgcagctccg cgacctgcac
     1201 cacccggtca cggggaacgt aggcgatgag ccgggagttg atccggcggg caaaatcctc
     1261 caccaggtcc cgttcgccct cgatgttctt tgcgttgcag atcacgcccc cgagcgagca
     1321 cctgctgcgc acccggcgcg agaggcgggc gatggccttt gcgatgttgt ttgccgcgta
     1381 gatcgacatc agctcgccgg atgtgaccag gtagatctcc tgcgcgtatc cctcgcgcat
     1441 cggcatcgca aacccgccgc agacgacgtc gccgaggaca tcgtagacga tcacgtcgcc
     1501 tttgagggcg tcaaggcgtt cgagaagctg gaacgtcgcg atgatgcccc gcccggcgca
     1561 cccgatcccc ggctcgggcc cgcccgcctc gacgcagcgc accccccgga acccccggta
     1621 gaccacatcg tcgacggtga tcccctcatc cccgcgctcg cggatcaggt cgagcacggt
     1681 cgggatccat gtcccgtgca tcagcatgcg ggtgctgtcg tgcttggggt cgcagccgat
     1741 ctgcaggatg tcgagcccct cccccgcgag cgctgccgag aggtttgccg cagtggtgga
     1801 tttcccgatt ccacccttgc cgtagagggc gatctgtttc atcgttctta ttatgcgctt
     1861 gcaagggtaa ttaggtgtcg gatgagactt ccatgcattt tatatataaa gacttcttaa
     1921 gattagaatt aattagttaa gggttatttc cttattaatc cagataatat tcgatatttt
     1981 acgtatttga ttgatataat cagttttaat atattagaaa actatataac aatgaattta
     2041 gaaccattga ttaaccggag ggaatgcgaa ccatggaccc acgatttcga tcacggttga
     2101 ttgcggcgat cgtactcctg atcgtcgttt gttcggcctt ttctgtgagc ccggttgccg
     2161 gattccgtct ggagaacagg gatgggagcg gcaccgatgc gacacttgca gaggctctgg
     2221 tgctgcagca gagcaccagg atcagagaag agttcatcaa ggacatcact gtctacatcg
     2281 acagcagaaa cagagtgttt gcacagcagg gcacatacgg gaacacgagc ggcctctacg
     2341 tccctgccga ggacgccatc tacatccgct cggacaggca cccggcgcag gccgacgagg
     2401 ccttcgcccg gcaggtcggc taccgggtct accacaccat gcggtttagc gagagcacgg
     2461 tctttccggc gctggatgcc gggtccgggc cctgcatggc ccggctctcc atcccgcccg
     2521 gcgaggagag ggaggcggcg ctctttgccg aggccttcat gctctatcac gcctctcccg
     2581 cgctcctgaa agaggatgcc ccggcgacgt atgcctacat ggacctgctc gtaaagagcg
     2641 gcggggattg cgcggcggta gatggtctgt acacccacgg ccggccggcg tgaccggccg
     2701 atcatggggt tctccccggt caaacgatcc cgcggcgggg tgttatccca cgccgcctgg
     2761 atcatcgtca catcgatccc gggttctcgg gcgccacccg gtgctccccc ctcgcgggcg
     2821 ccgggagcat ccgccccggg agagccggtc aatcgaaccc tttcaggatg cccatgacga
     2881 gcatgatcac cgggatgatc tcgaggcgcc ctatccacat gaccagtatg aagaggacct
     2941 ttgccgaatc cgccatatcg ggcgagacga accccgccga tatgccgcag ttgcacatgg
     3001 cggagacaac ctcgaatatc acgttgctcg aatcgaaggc cgtcggctgc aggtgcatca
     3061 ccagcaccgt ggcaacgaag atcgtgagga agtagagcat gatgatcagc atgttccgcg
     3121 agacctcgag atcggcgatg ttctttggta tcacccgacc gtcgtacttg aaggggacga
     3181 gcaccttccc ggagacgaac atccgccgga accaccagat caggctctgg gcgccgagga
     3241 ccacccggga gagcttgatg ccgccggcgg tgctccccga cgccccgcct atcaccatca
     3301 gcatcgagag gaagaggacc gtcacgctcg cccactcgtt tggagagacg atctgaaacc
     3361 cggtgctggt gatcgccgcg gccgacatga agagcgcgga gtggacggcg gtggtaagac
     3421 cggttgcgct gagggtgaca agatcccacg tgatcacgac gatgccgagc gcgacaagca
     3481 tgaagaggag gcgcgcctgc tggtcatcca ggacccgtat ccccctctgc cggtagagga
     3541 ggtaatagag cttgaacggc agggcgccgg cgatcatgac cgggacgatg aagacttcaa
     3601 ggagcgggtt gtggtagtag gggatgcctg ccgagtggac ggagaacccg ccggtggata
     3661 tcgccgtgag ggctatgttc accgcatccc agagaggtac ccccgagagg aggatgaggc
     3721 cgatcgacgc cgcggtcagg atcaggtaga tcttccacat ctccatgccc gtcgcgacga
     3781 cgctcggcat cagcgcctcc gagcgcccct ccgaccggta gaggcggaac tgggtcagac
     3841 cggtcctcga cgccatcgcg atcgtgaacg cgacgatacc gatgccgccg agccactgca
     3901 taagcgaccg ccagaagagg agcgtccgcg ggaggtcgtc gaccgaccgc atcaccgtca
     3961 gaccggtatc ggtccagccc gacatcgcct cgaagacggc gtcgaggtac gagacgccaa
     4021 gcccgaggct gaagggaagt gcgcttgcaa gcgccatgac gagccagatc agggcgacgg
     4081 ccatgagggc ggcggagagc ggcgcctcgc gctccccccg tggggttcgt acaagaagcg
     4141 tcccgaggag aaagagggtg atggggacgg agagcatggg gaggatcata tcccactcgc
     4201 ggtagatcac cgcgacgatg agcggcacgg cggtgccgat gctgatatac ctgaatatat
     4261 tgccgatgtc gggggcgatg gtcgagaact gctcgatcct atccatcggt acacgttctc
     4321 cgggcgctct tatctatctt ctctcccata acggttctgc cccggggcgc cggagaacgc
     4381 tcctctctca cgtgcggccg caccagcacc cgtcatatcc ggccggctct tcctcctttg
     4441 ccgcgcccgg agcaccctcg atagcgatgc gccggttgat gatccgccag gcgaggatga
     4501 gctgggccat gcccccggcc gtcaggtatg caccgttctt gacgaggtcg atgggctgca
     4561 cctgcgtctg gatctctatg ttctgctcca gtttcctcgc aacagcgtag tttaactccc
     4621 cgtcgaacgt gagggctccc atcggccgcc ctgagacgtc ttcggaggtg aactcgacca
     4681 tgaagctccg ctgcgagagg gagaggatcg ggtagaggtc gatcgagagg tcgacgttgt
     4741 ggatgctccg gtcgataccg ttctggcaga gagagacgtg gtagacgttg cgctcctcgc
     4801 tcaccttcct gccgtacttc gagaaggcta tcctgatgac cgcatccgca aaccggcact
     4861 gcggcgccac gtactgctcg tagtcgggct tgcgctcctc catctccttt atgacggcct
     4921 cgggcgaata cccgcgctgc tccacgtccc gtttgatctt ccaggcgcgc ttgacgtcgg
     4981 ggtccgggtc gacgtagagc ttgaagtcga ccaggtcacg gagcgtccgg gtgatgaacg
     5041 ggtgcaaccc ttcgacgatc aggatcttcg tggggctgaa gggcaccggc gggtcgaact
     5101 tcccgttgtc gtggttgtag accggttttg atatcgtctt tcccgccttg agatccctga
     5161 ggtgctcctc aagcaggtcg aaccggttcg cctcggggac gagcggcgtg ataccgagtg
     5221 ctttgcgctc ctcgcggtcg taccggtggt agtcgtcgat ggttatcatc gagacgagat
     5281 ccctgccgaa gatcgccctg atggcccggg tgaacgttgt ctttccgctt ccgctatctc
     5341 ccgctacgcc gatgacgaag acgtagggcg actctgcgat gactcgcttg aaatctgatg
     5401 ggggcatagg tcagctcaca gattggtctg tatcctgggc tgttaagatt tcggtgccgg
     5461 cggatgagcc gtctcgcccg cccaaaacca attacgggga gtgccccggg ctttcgatgg
     5521 caagtccccg ggcagtgtgc cgcaggacgg ctccccggga ggagagcgtg cccggcaaac
     5581 ccacgtcccg cctgcctctt cgcatggcgg gcagaaagag gtcgcatgtt cccgaccgcg
     5641 gcgggcggag tgccggggag gaagacctcc cggtgagccg tccggcccgc gggcatgctt
     5701 ccccctcatt ctccggagtc tgcttcttct caggagacga tgatacctgc ctcttcgtgc
     5761 ttcttcttga tagcatcggc gagccggtcc agcgccgcga agtccgcctc cttcgggtag
     5821 cccttgatgt agaccggttc gaggagctcg accttgagcc ggtcgagcat attcttcacg
     5881 gtgtcgaccg tctttccgcc ccagccgtag gatccgatca ccgtcgcgta ccgggtcttc
     5941 gggcgaagga ggtttgcaag gtatgctgcg tagaccgcct gcgggtgcgg gccgaatatg
     6001 accgtcggcg ccccgatcac gaccgtcgcg gcatccacga gagcctctgc aagatcgccg
     6061 atatcggtct tcgggaggtt gaacggctgc accgggactc ctcgctgcat cagggcatcg
     6121 acgaggtggt cgaccatcgc ctgggtgctc ccgtgcatgg agacgtacgg cagcaccacc
     6181 atgttcttca ccgcatcgtc cgtccagtca cggtatgcat ccatgatgaa cgccggcctc
     6241 ttgtggatcg ggccgtggct cggcgctatc atggcgatat cgcgcgcggc aagttttgcg
     6301 aggtggccct tgatgcttga gcggaacggc atcatgatct cggcgtaata gcgttttgcc
     6361 gactcgtaga tcacgccctc gttcgggaca tagaggctgc tcgtcgcata gtgcgacccg
     6421 aagaagtcgc aggagaagag gatccggtcc tcctgaaggt aggtgagcat cgtctccggc
     6481 cagtggaccc agggcgcgag gatgaactca agcgtctttc cccccagggg gagggtctct
     6541 ttatcgccga cgacgatggt ccggtctacc ggtatatgga ggaggtcgac caggaagtcc
     6601 cggcacttct ggttcgtcac cactttcgct tcggggaagc gggcgagcat cgccggaatc
     6661 gagccggagt gatcctgctc ggcgtggttt gcgatgatgt agtcgatcgt ttcagcgccg
     6721 gcccggtcaa ggttcgccat gagttccgcg gtctttacgg ggtcgacggt atcgatcagc
     6781 gccgtcttct cgctgccccg gatcaggtaa ctgttgtaag aggtgccgtc ggggagcggg
     6841 atcagttcat cgaagatcct gcggtcccag tcgatgaccc cgacggaccg gacgcccggc
     6901 acaatctcac gcgcggccat atctttccgc ctccatcggg cttattccgg tcattataca
     6961 gtctgtgtga gtaatttccg acatctttca cgctccacac cattcggcga ccggcatcgt
     7021 cgcgccacgc cgaccggtaa acgttaatcc ttctcaaaga ttgataacgg tttcgaccgg
     7081 cacctgcgcc gccggatcgc ggcgtccggt actccccatc gctctcatgg aaatgcgtga
     7141 ggtatgtgct acccttcccg ccgtgcctcc ggggagggga ggccggtctg tctatcctca
     7201 ccgatccccg tcttccgcgc aaggtttgca aagaggtgag cctgccgggt cggttccggg
     7261 agccggtatc ttcgtgcggt cgcgaggacg aggtcgaccg cctgaaaaag atcgatccgg
     7321 ttgcctacgg agacatagac cggtttcgtc ctctctttcg tccgcaccgc cataccgatc
     7381 gtctcgccgt cgcggatgat gggggcggtc gacccccggg tgaccccggg ctcctccacg
     7441 ctgccgacaa ggaggctctt tgcaaccccg accgtgggcc ggtcgagcag gacgcctatg
     7501 tggcttgcga tcccaagccc cctcgggtgg gcgattccct ggccgtcaaa gaagatgacg
     7561 tcgggctctg atcgcagctt ccggaacgcc ttgaggacgg cagggccttc ccggaaggtg
     7621 agaaggccgg ggatataggg aaacgacgtc tccgtgcggg cggcgacccg ctcgacgacg
     7681 gcaaggtcgg ggtagcccag ggcgacgacg accgcgtgga taacgtttga gcctttcacg
     7741 taggaggcgt cgacgccggc gacaagggag acctctccga tatcgcccgt gaggcggact
     7801 ctggtccgga gcgcctcctg gaggtgggcg gcctccgccg gcgtcaggtc gaacgggtgt
     7861 gtctctctga tctccatggt gactcctggt atgggtttac gtggtgttgt atacctgccg
     7921 gtatatatca gggggcatgc agcccaccat ggggatccgc ttcgtcatgc agttgttccc
     7981 ctgaagggca gacggggaac cgcaggcggc ggcgggaaac ctcgtgcgcc ggcggcacgg
     8041 gagcccggcc cgatcgcccg gggcgaggca tttttataca tggaactcgg gtatggcccg
     8101 gagtttcacc tccaggatgt gaacggtatg gcgcgatggg tgtgcagtgt ctgtgattat
     8161 gagtacaacg aagaggcggg ggaccccgcc gccggcatac caccgggaac gcggtttgaa
     8221 gacctccctg ccgactggcg atgtcctgag tgcatggtcg ggaaggatgc gttcgtgcgg
     8281 gtcgatgagg aggtggagaa gaatggtgaa aacgacctct aaagggccgg agacgaccgt
     8341 ggccgacgtc ctgatagcgg aactcgcggc atggggggtc aacctcttct tcggcatccc
     8401 cggcgcatcg tctctcccgc tcgtggatgc ggtccggagg aaccaggacg tccgctacat
     8461 cgtcgtccgg cacgaacaga ccgcggcgat ggcggcatcc gcctacaaca agttcaccgg
     8521 gaaggtagcc gcctgcctga ccatcgccgg tccgggggcg acgaacctcg cgaccgggct
     8581 ctacgacgca aaggaggacc acgcgagcgt actttcgctc aacgggcagg tgaagatgca
     8641 gtactccggc ccgggcggca tccaggagat cgaccaggat gcgttcttcc ggcccatcac
     8701 cgtcttcaac agcaccgtcg ccgacccgat gaccgcggtc aaactcctca cccgggcact
     8761 gcgctacgcc atcatcgccc gcggcgtcgc ccagctctcg gtcccgaacg atatccaggc
     8821 cgcaccgctc aaggccgcct actgcgagcg cgagacctgc cttccctcga cctgtgtcgc
     8881 gccgaccgac gaggccgtcc gggcggtggt ggaagcgatc gacggtgccc accgcccggt
     8941 catcgtcgcc gggttcgggg ccctgcacgc ggccggggcg gtccttgacc ttgcaacaaa
     9001 gatccaggcg ccgatcgtca ccaccctccg cggaaagggt atcctccccg acgataacga
     9061 atgggtcgcc ggggtgcacg gcgagctcgg gacgccccac gccctgatgc tcgtcctgaa
     9121 gtcggacctc gtgatcgcct gcggcatgag tttctccgac cacaccggca taccgccggg
     9181 taagcgggcc gtccagatcg acatcgatcc gctccagctc gggaaacatc cgcttgcggt
     9241 aggggtctac ggcgactgtg cccttgctct tccccggatc gccgggcagg tccgggcgcg
     9301 ggaggacccg gatacccggc agtggctcgc ggagaagaaa cgggagtggc tcgaccggct
     9361 cgaccaggag gccgacccgg aggccacccc catccgcccg ccctacgtca tgaaggtcct
     9421 ctccgacctc ctgccggagg atgcaatcat atccatcgat gtcggtgaca accagtggtg
     9481 gttcgggcgg aacttccgta tgaaacggca gcggttcgca atgtccggct acctcgggac
     9541 gatgggcttt gggctccccg gggcgattgc ggcaaaactc gcctaccctg atgcaaccgt
     9601 cgtctgcatc gtcggggatg gaggtttctc gatggtcatg ggcgatctcg tcacgaccat
     9661 caaatacgat ctccctatcg tcgtggtggt cctgagcaac cgcgaacttg ccatgatccg
     9721 cgaggagcag cgggaggagc actacccgcc cttcgggatc gatctcacga accccgactt
     9781 tgccgcctat gccgaggcct gtggaggcgc cgggattcgc gtgacccggc cgcaggacct
     9841 ccccggcgcg gtcagggagg ccctgaagat gaacacgtcc gtcgtcatcg acgtcgagac
     9901 cgatccggaa cggttcccgc agaaaaaaaa gtgatatcgg tttactcgcc gcaacagagg
     9961 ttgtcgtaaa ggatcgctgc ggcgaccgcg cctatgatgg gcccgacgat gtagatcggg
    10021 aagaactccc agaggttttg cccggcaagc agccaatccc cgaggtacgg cccgaacgta
    10081 cgcgcggggt tgagcgatgc gccggtcagg ttcccggtcg tcgtgatgat gccggcgacc
    10141 acgagaccga ccacgagacc ggcaaaaccc ggtgtggccc gttcgtcgac ggcggcgccc
    10201 atgatgacga gcatcaggag gaacgtcccg atggcctcga tggcgatcgc ctgcaggtag
    10261 ccgatgccgt ggaagggcgc cgttgcaccg aggcccccga tcgtgacggc gtccgggccg
    10321 atagcccagg caaagagaag gcttgcggcc gcggcaccga ggagctgcgc ggcgatatag
    10381 ggacctacgt cgcggcccgg gaaccgcccc gtcgcaaaga gggctatcgt caccgccggg
    10441 ttgatgtgac agcccgatac ccttccgaa
//
LOCUS       DAOSII010000065        10451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_6232_length_10451_cov_21.531166, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000065 DAOSII010000000
VERSION     DAOSII010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10451
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6232_length_10451_cov_21.531166"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2549)
                     /locus_tag="PLV90_06250"
     CDS             complement(<1..2549)
                     /locus_tag="PLV90_06250"
                     /inference="COORDINATES: protein motif:HMM:NF023252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOF96754.1"
                     /translation="MRECTPIWNSTLQKQTAIILLLLIAAPAAAGDFGGITLPNYKHI
                     DIQMANGGSKYVKFDGGGLNALHITTDPTDHYGQVTETDSQSGTFYFSDTGGRGFFDD
                     LILMVAVQGEVPDNFKVHIKASGYQWEPIPVLNKPPTMDEATHNEGAIDRNFTKDDFI
                     YAPQTWRPCKENYPIYYGQDMSKNNPEDRFYIMFIDLGVGAIGPNSFLDGLTDEGCAK
                     IEYSFENMETFAAFNAYGWCNQSNQGQGISWSNDLTGFAETGVSGYSVKGTKRSGDTG
                     GSGDDRSGSGIQGSNLNASTTGSINGTLLAFTAPGPAEPLAAGATAELRFPVAIPENA
                     TVDRGLLFVYMTDAKEAAAGADITPTFRFDGKPVTPAVTRDLQGTGSAPMAGTFSLAI
                     PPEMLAAGDHTLTVQMDAEPGATCRIPAALLVLVAADPGKPRIDYWIADGCDDVLADL
                     WEGTTADDATTKVKFDGAIDRARASAAHLYVVSTAGSGQGGTGNRVALNAWDWLNPLQ
                     EGPAGVWTATLDALPFIRASDNTVEVQSYPVGAAGDHLQNRNAILVVRYEAESASPGI
                     EVSSGAGAAPEEEIGSFALAPEEEVRYIAEDGSLVLTFPAGCRATNSTGGAVAEVTVH
                     AVRGLLPPPGMAMERIYRIAPDNTTTGTPFTLAAATPGTARNATWYRYDAAGAAWQPI
                     ETTLASGTATVSVKAFGTYGMAAPLPASFTVGSNPAGARILLDGAYLGRVTPATISPI
                     EPGNHTLRLELEQFTPYEADITVPDNTTLFADLDGLARLKFDEPQREPGLDRTGGVYV
                     MSRPSGAAISIDGKRMTLTTPRVILGIREGSHTVQVVKEGQSFDTPTKAVWV"
     gene            complement(2806..5175)
                     /locus_tag="PLV90_06255"
     CDS             complement(2806..5175)
                     /locus_tag="PLV90_06255"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOF96755.1"
                     /translation="MAFALIALPAAADSGIQLTATEPVAEISDDPGKESASVPEHAPV
                     GMSQNYETPAGQLIGPAPLPAPRHIFFNVANDAGVKHNLDGAAYGGPNGTYYIKADGG
                     GLNELHITNDAAAPAGQVTAGTDPSGVFWVANTGGRGYHDNVILLVSVNGTIPDDFAL
                     RIRSSGYVWAPAPPGEYSPELPSEYQYADGAVDELFSRDDFIYGPQAWKPGPGDLEVP
                     SLPLYYGQEIDDPSAASHLIFVDLNVGNLKNGVYPGAALTDNGGAKVEFAFTNLSTHA
                     AFNAYGWCSASNQGQGISWTNRVAGAESSGYTVAGVPPAPPVAAFSANVTAGDAPLTV
                     RFTDLSTGNPTSWAWDFEDDGVIDSVEQNATFTYAAAGTYTVNLTVANAGGGDSAVKT
                     DLITVTPPPGQTVLPGYNNIFIRVANNAGAKYNAFCNNTYNVMFEGIGRGLNALHVST
                     DPAANFGQVTVTENQTGTFYATDSGGKGYEDDILLMVAVNGTIPDNFRLRITADGYTW
                     TPNPERNRAPSLDNVAYQPLSLDETFTKADFIYGPQTWKPTGNEADYPLHAGQDMNDA
                     ENTFLLMFIDLNAGVLRPNEALVNRGAVRINYDLDNLESFAAFSVYGYCRNPNNGDDV
                     IAWTNALSSDKVMSGYSVIGNAGKPVAAFSADRTGGWAPLSVAFSDLSAGQPTAWSWS
                     FGDGATSTGQHPVHTYEAAGNYTVRLAVTNGKGSDTLVKQGYITVSNVKGDFNGDGTV
                     DIGDVAKVACMVVGKTGTDPAADFNGNGAVDIGDAAKIACYFVGKIATL"
     gene            complement(5434..9657)
                     /locus_tag="PLV90_06260"
     CDS             complement(5434..9657)
                     /locus_tag="PLV90_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOF96756.1"
                     /translation="MTGDKRIQNGPWQGRIAVLLLILAIIAVPAVALGADLASNTTEP
                     TVGIPDEISPSVDVEDNGTPEPTLTEMVEEEVTLAAIEQLPQYIDIDTTPGIYAVIPS
                     NNNVLLQISNDGNARFNDYGNNTYHFFSPDQNAGQGMNALHITIDPAESSGQVTFSSD
                     QAGVFYLTDTGGRGWDDDGILMLAVNGTIPDSFRVNIKASGYQWTPVPENAKPAFDDI
                     IYVSEALNETFTKDDFLYGPQIWRPCAAANYPIFDGQDMADTENTFSILFIDLNAGIL
                     GVNTLSQPSFSGQSVTDNGAIKVEYSFENLPTLAAFDAYAYTVSSNQGQGIRWTNCLS
                     TSGSSGYAVIGQPLPEAPIADFTASATSGAVPFFVRFNDTSTNSPTAWEWDFGDGATS
                     TAQNATHTYMAAGTYSVNLTVTNEAGTDSKIKQDYITVGETPSGKGLADTAWPKFGYD
                     LQNTGQSPYLGPQTATIKWTYTISIEDPAGQPAIGSDGTIYIECIDRHLYALNPDGTP
                     KWNFTAGDRLYGSPAIGKDGTIYIGSTDKNLYAINPDGTLKWTYTTDSSISESSATIA
                     PDGTIYIGTTGYQKNTLHAISDDGTQGTTKWTFNAGQLYSTPAVGTDGTIYIGSLDKN
                     LYALNPDGTLKWTYTTGGKMQRTSPSIGTDGTIYIGNADNNLYAINPDGTLKWTYTFG
                     SYIYSSVAIGSDGTIYTGNNDKKVYAINPDGTLKWDFTTENMIRSTPVLGSDGTIYIG
                     SYDRKFYALNPDGTLNWMYTTGGGINCHAAIGSDGTLYIGTTENIIYAFKDPVTPPTA
                     DFTANVTSGDAPLAVAFTDTSTGAITSWAWDFGDAATSTEQNPTYTYTTPGTYTVNLT
                     VSNEAGSNSMVKTGYITVTELYVPQPARIVVTPPAAELAVGATQAFAATVYDTEERTM
                     DDAAVTWASSNETVGMVNTAGLFTARAPGTTTVTAAYANVAGTAVATVTLPKGDQTQD
                     TPLDIPGCNVTPGDDGKSQVSINTTTTNATVNGTTIRIEEDNFTLTIETEGAPTNESG
                     TVNGTVASIRLDTNPIVTDLASVGTVNASVSLNLTGLPQGAGLTTTVSQNVSADARSA
                     FQLAASAGGLALGDVAYTLNIVRTNLENGQDIAGATIRMAVSPAWVAAHGGVDAIRII
                     RFAEDGTKEVLATTLVGTDADGNMIFEAASPKGLSAFGLAAVSAASQPAGSTSSGGGG
                     SSTAVGAASNLKAGSSATLPMQMTAVSAVTLTAKTDIKDAMVTMAKGSLPQAAQPPAG
                     VVYQYVQATLYKAAEADLAAVQFRFAVPTAWLAEQGCTAGQIALLQYTADGWKNVPVE
                     AIGEENGNAIFTASPDGFGLFAVAATGEAPGTAGDVTPGTTSSVTPTGTKDTVAHAEG
                     TTATPKATPLPVWAAVLALGGALLLVRRP"
     gene            complement(10036..>10451)
                     /locus_tag="PLV90_06265"
     CDS             complement(10036..>10451)
                     /locus_tag="PLV90_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HOF96757.1"
                     /translation="NQFRIMFVDLKTGVLHGYPIRVNYTIENLQTMAAFNVYGYSQNV
                     DEVNDYLTTWTNRLIGNTGEFSGWYVYGTPPESVKGDFDGNGIVDIGDVSKVAYMVVG
                     KTAADPAADFNQNGKVDIGDAAKIACYFVGKIGEL"
BASE COUNT         1739 a         2980 c         3395 g         2337 t
ORIGIN      
        1 acccagaccg cctttgtcgg ggtgtcaaac gactggcctt ccttgaccac ctgcaccgta
       61 tggctccctt cccggatgcc gaggataacg cggggcgtcg tgagcgtcat cctcttcccg
      121 tcaatggaga tggcggctcc cgacggccgg gacatgacgt agacgccgcc ggtccggtcc
      181 agccccggct cccgttgcgg ttcgtcgaac ttcaggcggg caaggccgtc gaggtccgcg
      241 aagagtgttg tattgtccgg gaccgtgata tcggcctcat acggggtgaa ctgttccagt
      301 tccagccgga gggtgtggtt cccgggctcg atggggctga tcgtcgccgg cgtcaccctg
      361 ccgaggtatg cgccgtcgag gagtatccgc gcgccggccg ggtttgagcc gacggtgaag
      421 ctcgcgggca ggggcgcggc catcccgtag gttccgaatg cctttacgga tacagtcgcc
      481 gtcccgctcg cgagggtggt ctcgatgggc tgccaggccg ctcccgcggc atcgtagcgg
      541 taccacgtcg cgttccgggc cgttccgggg gttgccgccg ccagggtgaa cggggttccc
      601 gtcgtcgtgt tatcgggggc gatccggtat atccgctcca tcgccatccc cgggggcggg
      661 aggaggcccc gcactgcgtg gaccgtcacc tccgccaccg caccgcccgt gctgttcgtc
      721 gcccggcacc cggccgggaa cgtgaggacc aggctcccgt cttctgcaat gtagcggacc
      781 tcttcttccg gcgcgagggc aaacgacccg atctcctcct cgggggctgc ccctgcgccg
      841 gaggacacct cgatgccggg ggacgcgctc tccgcttcat accggacgac cagtatggcg
      901 ttccggttct ggaggtggtc gccggcggcg ccgacgggat aactctgcac ctccacggtg
      961 ttgtcggatg cccggatgaa ggggagggcg tcaagggttg cggtccagac gccggccggc
     1021 ccttcctgga gggggttgag ccagtcccag gcgttcagcg ccacccggtt ccccgtgccg
     1081 ccctggccgg acccggcggt gctcacgacg tagaggtgcg ctgcggatgc ccgggcgcgg
     1141 tcgatggcgc cgtcgaactt caccttggtc gtggcatcgt cggcggtcgt cccctcccag
     1201 aggtccgcga ggacgtcgtc acagccgtcc gctatccagt agtcgatccg gggttttccg
     1261 ggatcggcgg cgacgagcac caggagggcg gcaggtatgc ggcaggtggc cccgggctcc
     1321 gcatccatct gcaccgtcag ggtgtggtcg ccggcggcga gcatctcggg cgggattgcg
     1381 agggaaaacg tcccggccat cggggccgaa ccggtcccct gcaggtcgcg ggttacggca
     1441 ggcgtgaccg gcttgccgtc gaacctgaac gtcggcgtga tatcggcgcc ggccgccgcc
     1501 tcttttgcat cggtcatgta gacgaagagg aggccgcggt cgaccgtggc gttctcgggg
     1561 atggcgaccg ggaaccggag ctccgccgtc gcgcctgccg ccaggggttc cgccggcccg
     1621 ggtgcggtga aggcgaggag ggtgccgttg atcgaaccgg tcgtcgatgc gttcaggttg
     1681 ctgccctgta tcccggagcc gctcctgtcg tccccggatc ctcccgtatc gccgctcctt
     1741 ttggtgcctt taaccgagta gccgctcacg ccggtctctg caaaaccggt gaggtcgttt
     1801 gaccacgata tcccctgccc ctggttggac tggttgcacc agccgtaggc gttgaacgcc
     1861 gcgaacgtct ccatgttctc aaaggagtac tcgatctttg cacacccctc gtcggtcagc
     1921 ccgtcaagga aactgttcgg gccgattgca ccgaccccga gatcgatgaa catgatgtaa
     1981 aaccggtctt cggggttgtt cttgctcata tcctggccgt agtagatggg ataattctcc
     2041 ttgcagggtc tccaggtctg cggcgcgtag atgaagtcgt ctttggtgaa gttccggtcg
     2101 atcgcgcctt cgttatgcgt ggcctcgtcc atcgtcggag gcttgttcag gaccgggata
     2161 ggctcccatt ggtagccgct tgccttgata tggactttga agttgtcggg gacctctccc
     2221 tgcaccgcca ccatcaggat gaggtcgtcg aagaagcccc ggccgcctgt gtcggagaag
     2281 tagaaggtgc ccgactgact gtctgtctcc gtcacctgcc cgtaatgatc ggttgggtcg
     2341 gtcgttatgt ggagggcgtt gagcccgccg ccgtcgaact tgacgtactt acttccgccg
     2401 tttgccatct ggatgtcgat gtgtttgtag ttggggaggg tgatcccccc gaagtcgccc
     2461 gccgcggccg gtgcggcgat gaggagcagc aatatgatgg ccgtctgctt ctgcagcgtg
     2521 ctattccaaa taggtgtaca ttctctcatg ggattacccg ccgttctgcg atgatgctgg
     2581 aaaagccaac tcaggatcag cacccggaag cccgcatgtg catggatccc ttttcgctga
     2641 tgaggtaagg tgcaacagcg gagtataaga ccgttattaa tttggatggg tataaatgaa
     2701 tcatcttaac atacttcata cggcgctaaa aataatgatt ttttattacc ggttcccggt
     2761 gggcaccatg ccgatacccc gccggcctgt ccgtttcatg caggatcaga gcgtcgcgat
     2821 cttcccgacg aagtagcagg caatctttgc cgcgtccccg atatcgaccg caccgttccc
     2881 gttgaagtct gcggcaggat cggtccccgt cttcccgacg accatgcagg caacctttgc
     2941 gacatcgccg atatcgacgg tcccatcccc gttaaaatcg cctttcacgt ttgatacggt
     3001 gatataaccc tgctttacga gggtgtcgga gcccttgccg ttggtgacgg caaggcgcac
     3061 cgtatagttg cctgccgcct cgtaggtgtg gaccgggtgt tgcccggtcg aggttgctcc
     3121 atcgccaaac gaccacgacc atgccgtggg ctggccggcc gacaggtccg agaacgcaac
     3181 gcttaacgga gcccatccgc ccgttctgtc ggccgagaac gccgccacgg gctttcctgc
     3241 gttaccgatg accgagtagc cgctcatcac cttatcggag gagagggcgt ttgtccaggc
     3301 gatcacatcg tcgccgttgt tggggttcct gcagtagccg tatacgctga aagcggcaaa
     3361 cgattccagg ttatcgaggt cgtagttgat cctgaccgct ccccggttca cgagagcctc
     3421 gttcggccgg aggacgccgg cgttgaggtc gatgaacatg aggaggaagg tgttctccgc
     3481 gtcattcata tcctggccgg cgtggagggg ataatccgcc tcgtttccgg tcggtttcca
     3541 ggtctgcggg ccgtagatga agtctgcctt tgtgaaggtc tcgtcgaggg agaggggttg
     3601 gtacgccacg ttgtcgaggg agggcgcccg gttccgctcg gggttcgggg tccaggtata
     3661 cccgtccgcc gtgatgcgga gcctgaaatt gtcggggatg gtgccgttga cggcgaccat
     3721 gaggaggata tcgtcctcgt atcccttccc gccggagtcg gtggcataga aggtgccggt
     3781 ctggttctcg gtcaccgtca cctggccgaa gtttgcggcc ggatcggtgg agacatgaag
     3841 ggcgttgagg ccgcggccga tcccttcgaa catgacgtta taggtattgt tgcagaacgc
     3901 gttgtacttc gccccggcgt tgtttgccac ccttatgaag atgttgttgt acccggggag
     3961 gacagtctgc ccgggaggcg gcgtaacggt gatgagatcg gtcttgaccg cggagtcgcc
     4021 gccgccggcg ttcgctacgg tcaggttcac ggtgtaggtg ccggcggcgg catacgtgaa
     4081 cgtggcgttc tgctcaacgc tatcaatgac gccgtcgtcc tcgaagtccc aggcccagga
     4141 tgtcgggttg ccggtggaga gatcggtgaa ccgcaccgtg agcggagcgt cgcccgcggt
     4201 gacgttggcc gagaaggccg cgaccggtgg ggccggcggc acgccggcga cggtgtaacc
     4261 gctggattcg gctcccgcta cccggttcgt ccaggatatc ccctggccct ggtttgaagc
     4321 gctgcaccag ccgtaggcat tgaatgccgc gtgggtggag aggttggtga acgcaaactc
     4381 gaccttcgcc ccgccgttgt cggtcagggc ggccccgggg tacacgccgt tcttgaggtt
     4441 tccgacgttc aggtcgacga agatcagatg ggatgctgcg gagggatcgt cgatctcctg
     4501 cccgtagtag agcggcaggg acggtacctc gagatcgccc gggccgggtt tccaggcctg
     4561 cgggccgtag atgaagtcgt ccctggagaa gagttcatct acggcaccgt cggcatactg
     4621 gtattcggac ggcaactccg gcgaatattc accggggggt gcaggcgccc agacatatcc
     4681 gctcgaacgg atgcgcaggg cgaaatcgtc gggtatcgtc ccgttcaccg agaccaggag
     4741 gatgacgttg tcatggtatc cccgcccgcc ggtgttggcg acccagaaca cgccggacgg
     4801 gtcggtgccc gcagtgacct gccctgcggg agcagccgcg tcgttcgtga tatggagttc
     4861 gttcaggccg ccgccgtccg ccttgatgta gtaggtgccg ttcggcccgc cgtatgcggc
     4921 gccgtccagg ttgtgtttca ccccggcgtc gtttgcgacg ttaaagaaga tgtgcctggg
     4981 tgccgggagg ggcgcggggc cgatgagttg cccggccggt gtctcatagt tctgcgacat
     5041 cccgaccgga gcatgctcgg gtacggaggc gctctctttg ccgggatcgt ccgatatctc
     5101 cgcaaccggt tctgtcgcag tcaactgtat gccgctgtcc gctgccgcag gcaaggcgat
     5161 aagggcaaag gccaatacca gggcggatat ccgcctccat gatacggtgt tttgaatata
     5221 ctcatcaatt cccatgacgg ccgctccttt cgccaggatg gtttctgcat tcgcagggac
     5281 gccagccgag caatgcaatc attctggttt acgctatatg agcgtttcta atcccgaagg
     5341 gattaataag gtttgtttta cgaaattctc tgatctgcaa agtcaatcgg atgaatactc
     5401 ctgatgcccg cctgcggaga aaaaaaggtg tgatcagggt ctcctcacga ggaggagagc
     5461 tccgccgagt gccaggaccg ccgcccagac cgggaggggc gtggccttcg gcgtcgccgt
     5521 ggttccctcg gcgtgtgcga ccgtatcttt cgttccggtc ggtgtcacgg acgaggtggt
     5581 acccggggtc acgtcgccgg ctgtgccggg agcctctccg gtcgcggcga cggcaaagag
     5641 gccgaacccg tcggggcttg cagtgaagat agcgttgccg ttctcctcac cgatcgcctc
     5701 gaccgggacg ttcttccagc cgtcggcggt gtactggagg agggcgatct gtcctgcggt
     5761 gcagccctgc tctgcgagcc aggcggtcgg cacggcgaac cggaactgga cggcggcgag
     5821 gtccgcctcg gccgccttat agagggtcgc ctggacgtac tggtagacca cgcccgccgg
     5881 gggttgtgca gcctgcggga gcgagccctt cgccatggtc accatggcgt ctttgatgtc
     5941 ggtcttcgcg gtgagggtga ccgccgagac ggccgtcatc tgcatcggga gggtggcgct
     6001 actgccggcc ttgaggttgc tcgccgcgcc gaccgcggtc gatgaaccgc cgccgcctga
     6061 cgaggtagac ccggcgggct gcgatgctgc cgagactgcg gcaagcccga aggccgagag
     6121 gccctttggc gatgcagcct caaagatcat gttcccgtcg gcgtcggtgc cgacgagcgt
     6181 cgtcgcaagc acttccttcg tgccgtcctc ggcgaaccgg atgatccgga tcgcgtccac
     6241 gccgccgtgg gcggcgaccc aggcggggct gaccgccatc cggatagtgg cgccggcgat
     6301 gtcctgtccg ttctcaaggt tcgtcctcac gatgttcagc gtgtaggcga cgtcgccgag
     6361 ggcaagcccg ccggcgcttg ccgcgagctg gaaggcgctt ctcgcatcgg cggagacgtt
     6421 ctgcgagacg gtcgtcgtga ggccggctcc ctgcgggagg ccggtcaggt tcagcgagac
     6481 ggaggcgttg accgtgccga ccgatgcgag atccgtgacg atcgggttcg tgtcgagcct
     6541 gatgctcgcg actgttccgt tcacggtgcc gctctcgttg gtgggagcac cctcggtctc
     6601 gatggtgagg gtgaagttgt cctcctcaat ccggatggtg gtcccgttga cggttgcgtt
     6661 ggtggtggtg gtgttgatgc tgacctgcga cttgccgtcg tcgcccgggg tcacgttgca
     6721 gccggggatg tcgaggggag tgtcctgggt ctggtcgcct ttcggcaggg tcacggtggc
     6781 gacggcggtg cccgctacgt tggcgtacgc ggcggtgacg gtcgtcgtgc ccggggcgcg
     6841 ggcggtgaag aggccggcgg tgttcaccat gccgacggtc tcgttcgagc tcgcccaggt
     6901 gacggcggcg tcatccatcg tcctctcttc ggtgtcgtag acggtcgcgg cgaatgcctg
     6961 cgttgccccg acagcgagtt ccgctgccgg cggggtgacg acgatgcggg ccggctgcgg
     7021 cacgtacagt tcggtgacag tgatgtaacc ggtcttgacc atggagttgc tgccggcctc
     7081 gtttgagacc gtcaggttga cggtgtaggt gccgggagtg gtgtaggtgt aggtcgggtt
     7141 ctgctcggtc gaggtcgcgg cgtcaccaaa gtcccaggcc caggatgtga tcgcgcccgt
     7201 ggaggtatcg gtgaaggcga ccgcgagtgg ggcgtcgccg gatgtcacgt tggcggtgaa
     7261 gtcggcggtg ggcggagtaa cgggatcctt aaatgcatag atgatatttt ccgtggttcc
     7321 aatgtaaagg gttccgtcgg atccgattgc cgcatggcaa ttaatcccgc cgccggtggt
     7381 gtacatccaa ttcagtgttc cgtccgggtt caatgcatag aattttctat cgtagctgcc
     7441 gatgtagatg gttccgtcag aaccgagtac cggcgtactg cggatcatgt tttccgtggt
     7501 aaaatcccat ttgagtgttc cgtcagggtt tattgcatag acttttttat cattatttcc
     7561 tgtgtagatg gtgccgtcgg atccgattgc aacagagctg tagatgtagg atccaaaggt
     7621 gtaggtccat ttgagcgttc cgtccggatt tatagcgtag aggttgttgt ctgcgttacc
     7681 aatatagata gttccgtctg taccgatact tggggaggta cgttgcattt tccccccggt
     7741 ggtgtaggtc catttgagcg ttccatccgg atttaatgca tagaggttct tgtccaaact
     7801 tccgatgtag atggttccat ctgttcccac agcgggcgtg ctgtataact gacctgcatt
     7861 gaacgtccat ttagtagttc cctgggttcc atcgtcggat attgcatgta aagtattttt
     7921 ttgataacca gtggtaccga tgtagatggt tccgtccgga gcgattgttg cggaagattc
     7981 ggatattgaa ctatctgttg tgtaagtcca tttgagcgtc ccgtcaggat tgattgcgta
     8041 gaggttctta tcggtgcttc ctatatagat ggttccgtct tttcctatag caggtgatcc
     8101 gtataaccta tctcctgcgg taaaattcca tttgggggtt ccatctgggt ttaatgcgta
     8161 gagatgtctg tcaatgcatt caatatatat ggttccgtcc gacccgatgg caggctgacc
     8221 cgctggatcc tctatgctaa tcgtgtaagt ccattttata gtggccgtct gcgggccgag
     8281 gtagggagac tgcccggtat tctggaggtc gtagccgaac ttcggccagg cggtatcggc
     8341 aaggcctttc ccggacgggg tttcgccgac cgttatgtaa tcctgtttta tcttgctgtc
     8401 ggtgcccgcc tcgttcgtca ccgtaaggtt cacggaatac gttcccgcgg ccatgtaggt
     8461 gtgcgttgca ttttgagctg tcgaggtggc tccatcgccg aagtcccact cccaggcagt
     8521 cgggctgttg gttgacgtgt cgttgaaacg cacgaagaac ggcacagccc cgctggttgc
     8581 gctggcggtg aagtccgcga tgggggcttc cgggagcggc tggcctatga cggcgtatcc
     8641 ggacgacccg ctggtgctca ggcagttggt ccagcggatg ccctggccct ggttcgagga
     8701 gaccgtgtag gcatacgcat cgaaggccgc gagcgtcggc aggttctcga aggagtactc
     8761 gaccttgatc gccccgttat cggtgacgga ttgtcccgag aaactcggct ggctgagcgt
     8821 attcacgcca aggatgcctg cgttcaggtc gatgaacagg atcgagaagg tgttctcggt
     8881 atccgccatg tcctgcccgt cgaaaatggg gtagttcgcc gccgcgcagg gcctccagat
     8941 ctgcggtccg tagaggaagt cgtccttggt gaacgtctcg ttcagggctt cggaaacgta
     9001 gatgatgtca tcgaacgctg gcttggcgtt ctcgggcact ggcgtccact ggtagccgct
     9061 cgccttgatg ttgacccgga aactatcggg tatcgttccg ttgacggcga gcatcagaat
     9121 gccgtcgtcg tcccagccgc ggccgccggt gtcggtgagg tagaagacgc ccgcctggtc
     9181 gctgctgaat gtgacctggc ctgaagattc cgccggatcg attgtaatat gaagagcgtt
     9241 catgccctga ccggcgttct ggtctgggct gaagaagtga taagtgttgt tgccgtaatc
     9301 gttgaaccgg gcgttcccgt cgtttgagat ctgcaacagc acgttattgt tcgatggtat
     9361 tactgcgtag atgccgggag tggtatcgat gtcgatatac tgcggcaact gctcgatcgc
     9421 tgccagcgtc acctcttctt cgaccatttc ggtcagtgtt ggttcgggtg tgccattatc
     9481 ctccacatcg acactcggag atatctcatc gggaatcccg acagtcggtt cggttgtgtt
     9541 cgacgccagg tcggctccca gcgccaccgc cggtacggcg atgatcgcga ggatcagcag
     9601 caggacggct atccgcccct gccatggacc gttctgaata cgtttatccc ctgtcataat
     9661 acttcccttt caaatcgaga attctcgaag aagtttctgc ggaaccggca ccccatgtgc
     9721 ctgcatcgct ataaccgctc tctgagaatg agtaaatttt taatcgagag gtataaggtt
     9781 gttgctaaat cgggctgttt taaatgagtt tagttaacaa atttgagttt tcactaaaga
     9841 taatgtatct tctgcagacg tggcatactc ctcctgccca caaagaaccg gagagctaaa
     9901 gggtacgaca ggtggtggcg atgaaaaaat agacgatctg gagtcaggtg cggccttcat
     9961 ctccggtgcg tctgctccgc cggcgtcggg atcagcaccg ggccgtattt tgatatggaa
    10021 tctcgcggga aggggttaga gttcccctat cttcccgacg aagtagcagg cgatctttgc
    10081 cgcatccccg atatcgacct tcccgttctg gttaaagtcc gcggccgggt cggccgccgt
    10141 cttcccgacg accatatagg ccaccttcga gacgtcgccg atatcgacga tgccgtttcc
    10201 gtcgaaatcg ccttttacgc tttccggagg cgtgccgtaa acataccaac cgctgaactc
    10261 gccggtattc ccgatcaggc ggtttgtcca ggtggtgagg taatcgttca cctcatccac
    10321 gttctgagag tacccgtaga cgttgaaggc cgccatcgtc tggaggttct cgatcgtgta
    10381 attcaccctt atcggatagc cgtggagaac gccggtcttc aagtcgacga acattatccg
    10441 gaactggttc g
//
LOCUS       DAOSII010000066        10420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_6260_length_10420_cov_42.556778, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000066 DAOSII010000000
VERSION     DAOSII010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10420
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6260_length_10420_cov_42.556778"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(332..1858)
                     /locus_tag="PLV90_06270"
     CDS             complement(332..1858)
                     /locus_tag="PLV90_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOF96758.1"
                     /translation="MTGNIIGLTKMDLSNARSPRAAAFKGNKSLTNLAPVPGFKAEIG
                     LELVSENYTAPMMVASPDDGTGRLFAVDQIGVAKIVDADGTTLPEPFLDLRDNIVKLS
                     PKYDERGLLSIAFHPDYRKNGRLYVFYSAPLRPEAPGGWSCTNHISEFRVDPDDPNRV
                     DMSSEKVLMYIDKPYENHNGGQLVFGPVDGYLYISLGDGGKANDVGNGHTPGIGNAQD
                     LTKIYGKILRIDVNGTTAGSVPAQKSPTGDVNVDQPESPPEPTWTTFAGSLYGIPPDN
                     PFAKTQPELLDSYAYETVPPEIYAYGLRNPAYMAFDSGGNNALFVGEAGQNLFESVDI
                     IVRGGNYGWNIHEGTHCFDTKKPLQPPESCRTTGYRDEPLIGPIFEGGHDLGLVIVGG
                     YLYRGPSVPGLQGRCIFGYWGDSRLVGNGILLVATPPEEGAGGERPMDASGLTPEGNT
                     MWRAQKLKVTGKKDEFLGMFVRGFGEDINRDMYVVTNDTGGPDASTSTGKVWKIVPPG
                     "
     gene            complement(2037..3662)
                     /locus_tag="PLV90_06275"
     CDS             complement(2037..3662)
                     /locus_tag="PLV90_06275"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOF96759.1"
                     /translation="MVARETRMVEEKSGKRLSLISAVIIAIIAYLIFLAVVIVPLQGG
                     EISSTAIFADNLSESNASYATSNIPVQGVGDVSRSAVIAFAMLTHIVFANLQVGGAWI
                     IVATALLYFRYQRMRYRNLARSLTLFTLILFSAGSTFAAGGMMAIIALFPDLAMNIFH
                     LYWWPVFIYFLLYGVVITLLFVFWFAWDRFRPGVHLALGFGYAISVFIQTITIDTLAA
                     GMLTPGVKAFSFTESGLLPMTLDQAMALWFNPTLWELTFHRVAAAVAFFGFLIATLAT
                     IHYIDQKDFAAKKQWDWVAAYGIAWGLAGLIMQPVLGQMYMGAIQDNVPSAFMMIMHG
                     PRAWAMLMMVTLLSGLFLAIGTYFLTRRDQLLSRPENRTIRTVFKGFLIMTAVSAFIL
                     VQPAWLGALFIDDPGAWINPIGGMPLKFVALFVMAAIGAAIAMLAVIILTSEDKEGQW
                     GYLTRGSLLAAFTAGILGTAIVNVMGFVREAARAPWTFYQIIPVPGGQAYATPIPLPV
                     IAGVWVIVLALSWAVFWYVAKVTAYHPTDEESV"
     gene            complement(3676..3801)
                     /locus_tag="PLV90_06280"
     CDS             complement(3676..3801)
                     /locus_tag="PLV90_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96760.1"
                     /translation="MNRNRSRRLTYAMWIAIAIYIGIAIYVFWHFGFTKLPPPPI"
     gene            complement(3798..5435)
                     /locus_tag="PLV90_06285"
     CDS             complement(3798..5435)
                     /locus_tag="PLV90_06285"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOF96761.1"
                     /translation="MTTPAGGASSAPDEHDSRGDTRKIVGILAALAIFIIVLYGYIIP
                     LQGGFISSTAIRADDLLGADPRFEAQMPIQEVDLGASGRSIFIAFVMLTHILFANLHL
                     GGAWVLLSLVILDLASRKERYARLGMSLALFNVILFSAGATFAAAGVLFFISLYPTFA
                     AQGFHIYWWPLLVEAILFGIEIFFVYTLWFTWGRIRGAWHVFLAVGYALSIYFQTLAI
                     DTFVSGMLTPGEKTITWGAPGLLGMPWIDYLQWWFNPTLWPLQFHRVAAAVSYFGFLI
                     ALLAMLHFRDRTDPPSKKYWDWAGSFGILWGLVGLVAQPLLGLWYMYSIFSHQNQAFT
                     NIMTGPRAWEMLMMIGLLSLLIVTVSVYFIERRERLLVRLGRHDIRNLFRAFAIVAAV
                     AGLVLVQPAWLGGIMQFDPGTWANPLGLMYYKHIAILVLVAIGTIIIGIDLLVLREHR
                     DKEWGNLSRASWMAALIAGVLGSWIVIIMGYVRESARSPWLFYGIVPVPGGQTYPTPV
                     PPFQIFVVWMIGLGLAFAVFWFTSRVTSYHPEREEEV"
     gene            complement(5794..6396)
                     /locus_tag="PLV90_06290"
     CDS             complement(5794..6396)
                     /locus_tag="PLV90_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HOF96762.1"
                     /translation="MRPNVILIVALLVVSAGVLAAGCTTPGENVTQPTAPPTGTETPA
                     VTATTVAETPAATGTTTAETPAATATTAAETPTTTETTAATTTTAATNATTGATTAAG
                     GGTATVDLTAQNIAFDKSTITVPAGAEVTVNFDNQDSGIPHNFAVYTDSSASQSIFKG
                     DTITGPATTTYTFTAPDEAGTYYFRCDVHPEQMNGDFVVQ"
     gene            complement(6446..7600)
                     /locus_tag="PLV90_06295"
     CDS             complement(6446..7600)
                     /locus_tag="PLV90_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOF96763.1"
                     /translation="MIRFTHLIHGEGPVSGPAAKHSHLAFEEGRRGPVVFWNITSRCN
                     LACTHCYLRSGPRRRREDELTTGEAKSLIDDLAAANVPLLLFSGGEPLMREDFWSLAA
                     HAQERGIPAVLSTNGTLITPTVARRLRDAGIGYAGISLDGATAATHDLFRGVVGSFDR
                     SVQALKYCIDAGLRCGVRFTVTKRNHSELGRLIALARSIGVHRFCVYWLVPSGRGGDA
                     HADLQVGPGEVQGVIDLIYRETLRTDPAAMEFLTVDAPQDGVCLLEMLKADAHPAYDR
                     TYRLLARMGGCSAGRRVANIDPSGNVYPCQFAQFREFLAGNIRDRPFSEIWSDPGNQV
                     LTAFREGEARPGSPCSTCDRQEVCGTGCRVRAYVRYGDLNSGDPLCLRHG"
     gene            complement(7601..8080)
                     /locus_tag="PLV90_06300"
     CDS             complement(7601..8080)
                     /locus_tag="PLV90_06300"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOF96764.1"
                     /translation="MNDRTLLIRLLSGVPLVEEPYRAIAEELDVTEAEVIARIESLLS
                     QGIIRRFAVRLDHRRAGIRVNAMVVWRVPPGDMMQVAEIMARSPEVTHCYERATVPGR
                     WDYNLYAVLHGRRRAGVDRSIARLSRAAGIDDCAVLVSTRELKRTPPGLALLAKEGR"
     gene            complement(8077..8577)
                     /locus_tag="PLV90_06305"
     CDS             complement(8077..8577)
                     /locus_tag="PLV90_06305"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HOF96765.1"
                     /translation="MTDRHLDILDRQLLEILQDTFPLAPRPWHAIGRRLGIPEGEVLR
                     RVRRLADEGIVLTVSPVIESARIGPVASTLIGMRVPADRVERCASIVNREPGVSHNYL
                     RDHDYNLWFTLAAPDSAALRRTVEDIAARAGVPPADLLDLPAVRRFKIGVRFRFFPDG
                     DGEGGI"
     gene            complement(8570..9655)
                     /locus_tag="PLV90_06310"
     CDS             complement(8570..9655)
                     /locus_tag="PLV90_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOF96766.1"
                     /translation="MERVLDAGGGPRIVSWNVTARCNLACAHCYIDADGEGSPGELDT
                     AEGMALIDQIAAVGRPILVLSGGEPLLRPDIFDLARYAAGRGLRVAMGTNGILVTDAV
                     AERLSDTGIRKVAVSIDSTDPGVHDAFRGVSGAWEKAVNGIRALRRAGVPVQIHTTIG
                     EPDIGEIDRIIAFGKSHGVRDYQFFFPVPTGRGEDLADLPPEVHEAVIGRILAHAREP
                     GFSVRPTCAPQFVRVAARMGLDLSQWGRGCIAGTAYCRITPVGDVTPCPYLPVPVGNI
                     RREAFSDIWHGSEIFAALRDPDSLGGKCGQCEYRRACGGCRARAYGVRHRRGDACGGP
                     VRPGDPDGDILAEDPCCPYEPGEITHD"
     gene            complement(9655..9999)
                     /locus_tag="PLV90_06315"
     CDS             complement(9655..9999)
                     /locus_tag="PLV90_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96767.1"
                     /translation="MVGTKKEVKIARILITVSVVFGFLVVVTLLVIGTGGIAGKAGPS
                     GISTTGALLLAAVKAAGTVFGLAAYLTIARGDLRKAGRYALVASILPPFDLIALFAGL
                     LALFSHREGGAG"
     gene            complement(10136..>10420)
                     /locus_tag="PLV90_06320"
     CDS             complement(10136..>10420)
                     /locus_tag="PLV90_06320"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HOF96768.1"
                     /translation="TVVNLTAQNIAFDKSTITVPAGANVTVHFTNKDSGVQHNFAVYD
                     SNLRSKSFFVGEIITGPAEANYTFTAPSDPGTYYFQCDIHPFMNGKFIAE"
BASE COUNT         1903 a         3194 c         3467 g         1856 t
ORIGIN      
        1 agtagtgttg ttcgcccctc cctgctgggc tgccacgcct ccgatgaggc acaggcagat
       61 cgctatcgca aggaaggcca tacgctgtgt atgaggtctc atacgaggcg gggggataga
      121 ggtcatatgt tatatacgtt ccgggatgcc gggggtgtga ggcactcccg gggatctgtg
      181 catgtgcggc cgttctcctc actcttgaga atccctcctc cccggcgagg cggcaggccg
      241 tgaccccggc cccgggccgg ggggcgatcg agcagcgggg agaggcacgc tctcttcctc
      301 ccccggcccg ggtcacgggg gatggcaccg atcacccggg cggcacaatc ttccagactt
      361 tgccggtgct ggtggatgca tccggccctc ccgtgtcgtt tgttaccaca tacatgtccc
      421 ggttgatatc ctcaccgaac ccgcgcacga acatgcccag gaactcatct tttttgccgg
      481 tgactttcag tttctgcgct ctccacatgg tgtttccttc cggcgtcagg cctgaagcgt
      541 ccatgggccg ctcacctccg gccccctctt ccgggggcgt ggcgacgagg aggatgccgt
      601 tccccacaag ccggctgtcg ccccagtagc cgaatatgca cctgccctgg agcccgggga
      661 cggagggccc acgatagagg tagccgccga cgatgacgag gccgaggtcg tggccgccct
      721 caaagatggg accgatcagc ggttcgtccc ggtatccggt cgtgcggcag ctctccgggg
      781 gctggagcgg tttttttgtg tcaaaacagt gcgttccctc gtgaatgttc cagccgtagt
      841 tcccgccgcg tacgatgatg tccaccgact caaagaggtt ctggccggct tcgccgacga
      901 agagcgcgtt gttgccgccg gagtcaaagg ccatgtaggc ggggtttcgg agcccgtagg
      961 cgtatatctc gggcgggacc gtctcgtagg cgtaagagtc gaggagctcg ggctgggtct
     1021 ttgcaaacgg gttgtcgggc ggtataccgt agaggctgcc ggcaaacgtc gtccatgtcg
     1081 gctcgggcgg gctctcgggc tggtcgacgt tcacatcccc ggtcgggctc ttctgggccg
     1141 gcacgcttcc ggcggtggtg ccgttcacat cgatccggag gatcttgccg tagatcttcg
     1201 tgagatcctg ggcgttcccg atgcccgggg tatgcccgtt cccgacgtcg tttgccttcc
     1261 cgccgtcccc gagggatatg tagaggtagc cgtcgacggg gccgaagacg agctggccgc
     1321 cgttgtggtt ctcgtagggt ttgtcgatat acatcaggac cttctcagac gacatatcca
     1381 cccggttcgg gtcatcgggg tcgacccgga actcggatat gtggtttgtg cagctccagc
     1441 ccccgggcgc ttccggtcgt aagggtgcgc tgtagaagac gtagagccgg ccgttcttcc
     1501 tgtaatcggg gtggaacgct atggagagca ggccgcgttc atcgtacttc gggctgagtt
     1561 tcacgatgtt gtcccggagg tccaggaacg gttcggggag cgtggtccca tctgcatcga
     1621 cgatctttgc gaccccgatc tggtccacgg caaagagccg cccggtcccg tcgtcgggtg
     1681 aggcgaccat catgggcgcg gtgtagttct cgctgacgag ttcaaggccg atctcggctt
     1741 taaagccggg aacgggtgcc agattcgtta aagatttatt gcccttgaac gccgcggcac
     1801 gcggcgatcg ggcgtttgaa agatccatct tcgtgagacc tattatgttg ccggtcatgc
     1861 tcaacaactc ctatcatctg atcttgaggt ttcgttacca ctacagggct caagtctcgc
     1921 ccgctgttcg cccgcgatgg gcgtgagggt cagggaagat gctccggcag gggtaacctg
     1981 tgccatgcgg ggcaggacat ggtatcaagt atgataaaga ttaccccgaa ttccattcag
     2041 acggattcct catccgtcgg gtggtatgcc gtcaccttcg cgacgtacca gaagaccgcc
     2101 cacgagagtg cgagcacgat cacccatacc ccggcgatca ccggcagagg gatgggggtt
     2161 gcgtaagcct ggccgcccgg gacggggatg atctggtaga acgtccaggg ggcgcgtgcc
     2221 gcctcccgca cgaatcccat gacgttcacg atcgccgtcc cgaggatacc tgccgtgaag
     2281 gcggcaagga gcgagccacg ggtgaggtat ccccactgcc cctctttatc ttcgctcgtc
     2341 aggatgatga cggcgagcat ggcgatggcg gcaccgatcg ccgccatcac aaagagggcg
     2401 acgaacttca gcggcatccc cccgataggg ttgatccatg ctccggggtc gtcgatgaag
     2461 agcgccccga gccaggccgg ctggacgagg atgaacgcgg agacggcggt catgatcagg
     2521 aaccctttga agactgtccg gatggtgcgg ttctcgggcc gggagaggag ctggtcacgc
     2581 cgggtgagga agtaggtccc gatggccagg aagagaccgg agaggagtgt caccatcatg
     2641 agcatcgccc aggcccgggg gccgtgcatg atcatcatga aggcggacgg cacgttatcc
     2701 tggatagcgc ccatgtacat ctgcccgagg acgggctgca tgatcagccc tgcaagcccc
     2761 caggctatcc cgtaggcggc cacccagtcc cactgtttct tcgccgcaaa gtctttctgg
     2821 tcgatgtagt ggatggtcgc aagggttgcg atcaaaaacc cgaagaaggc gacggcggcg
     2881 gcgacccggt ggaaggtgag ttcccagagg gtcggattga accagagcgc catcgcctga
     2941 tcgagcgtca tcgggagcag cccggactct gtgaaggaga acgccttcac ccccggcgtc
     3001 agcataccgg cggcgagcgt atcgatggtg atggtctgga taaagacgct gatcgcgtag
     3061 ccgaacccga gcgcgaggtg cacgcccggc ctgaaccggt cccaggcaaa ccagaagaca
     3121 aagagcaggg tgatgacgac cccgtagagg aggaagtaga tgaagaccgg ccaccagtag
     3181 agatgaaaga tgttcatggc gaggtcggga aagagtgcga tgatggccat cataccgccg
     3241 gccgcaaacg tcgaccccgc cgagaagagg atgagcgtaa agagcgtgag cgaccgggcc
     3301 aggttcctgt agcgcatgcg ctggtaacgg aagtagagga gtgccgtggc cacgatgatc
     3361 caggcgcccc cgacctgcag gttggcaaag acgatatggg tcagcatggc aaacgctatg
     3421 acggcggagc ggcttacgtc cccgacaccc tggacgggta tattggatgt ggcgtaactc
     3481 gcgttgctct cgctgaggtt atcggcaaag atggctgtgg aggagatctc gccgccctga
     3541 agcgggacga tgacgaccgc aagaaagata aggtaggcta tgatcgctat gatgactgcg
     3601 gatatgaggg agagcctctt tccggatttc tcttccacca tccttgtttc acgcgcaacc
     3661 atctctgtac ctcctctaaa tcggcggggg gggaagtttc gtgaacccga agtgccagaa
     3721 tacgtagatc gcgatcccga tgtagatcgc gatcgcgatc cacatggcgt aggtcagcct
     3781 ccggctccga tttcggttca tacctcctcc tcccgctccg ggtgatagga cgtcaccctt
     3841 gatgtgaacc agaagaccgc aaaagccagc ccgaggccga tcatccagac cacaaagatc
     3901 tggaacggcg gcaccggcgt cggataggtc tgccccccgg gcacggggac gattccataa
     3961 aacaaccatg gggagcgcgc cgactcccgg acatacccca tgatgatgac gatccatgaa
     4021 ccgagcaccc cggcgatgag tgccgccatc cacgacgccc gggagaggtt gccccactcc
     4081 ttatcccggt gctcgcggag caccaggaga tcgatgccga tgatgatcgt cccgatggct
     4141 accaggacga ggatggcgat gtgcttgtag tacatgagcc cgagcggatt tgcccaggtc
     4201 ccgggatcga actgcatgat ccccccaagc cacgccggct ggacgaggac gagccccgcc
     4261 accgcggcga cgatggcaaa tgcccggaag aggtttcgga tatcgtgccg tccgagccgg
     4321 acgaggaggc gctcccgccg ctcgatgaag tagaccgaga cggtgacgat gaggagcgag
     4381 agcaggccga tcatcatgag catctcccac gcccgcggcc cggtcatgat gttggtgaag
     4441 gcctggttct ggtgtgaaaa tatcgagtac atataccaca ggccgaggag aggttgggcg
     4501 acgaggccga ccagccccca gaggatgccg aacgagcctg cccagtccca gtacttcttc
     4561 gacggcggat cggtccggtc gcggaagtgg agcatcgcaa gaagcgcgat caggaacccg
     4621 aagtaggaga ctgcagcagc gacccggtgg aactggagcg gccagagcgt gggattgaac
     4681 caccattgca ggtagtcgat ccagggcatg ccgaggagac cgggcgcccc ccaggtgatc
     4741 gtcttctcac ccggcgtcag catccccgag acaaaggtat cgatcgcgag agtctgaaag
     4801 tagatcgaga gtgcgtagcc gacggcaagg aagacgtgcc aggcccccct gattctcccc
     4861 caggtgaacc agagggtgta gacgaagaag atctcgatcc cgaagaggat cgcctcaacg
     4921 aggagcggcc accagtatat gtggaacccc tgtgcggcaa acgtcggata gagcgatatg
     4981 aagaagagga ccccggccgc cgcgaacgtc gccccggccg agaagaggat gacgttgaag
     5041 agcgctaacg acatcccgag acgggcatat cgttcttttc tggaggcgag gtcgaggatg
     5101 acgagcgaga ggagcaccca tgcgcccccg aggtgcaggt tcgcaaagag gatatgagtg
     5161 agcatcacga acgcgatgaa gatcgaccgg ccggaggccc cgaggtcgac ctcctggatc
     5221 ggcatctgcg cctcgaaccg gggatccgct cccaggagat catcagcccg tatcgccgta
     5281 gaggatatga acccgccctg caggggtatg atataaccgt agaggacgat gatgaagatc
     5341 gcaagcgcgg ccaggatccc gacgatcttc ctggtatcgc cccttgagtc atgctcatca
     5401 ggtgccgatg atgctccccc ggccggcgtg gtcatccccc ccgagcccgg gcatggggct
     5461 caccccatat aaacgttatg ccgggcgaca ccgggggcga agaaggggga gatcggggga
     5521 tctcccgccg gtggtgcgta tcactgcacg acgagatcgt ctttcatctc ttcggggtgg
     5581 acgccgcagc ggaagcagta ggtaccgggt ttatcgggtg cgatgaaggt gtaggtggtc
     5641 gtcgccggga cttccggctg cgaggaagga gcatcccggc aactgccggg gatactatca
     5701 ggaatagcag gggtttcacg gcggcccccg atcggatcag gcggtcataa cggttaccgg
     5761 aagcggagat gggaaaaaga gtgggtggat tgatcactgc acgacgaagt ccccgttcat
     5821 ctgctcgggg tggacgtcgc agcggaagta gtaagtaccg gcctcatcag gcgcggtgaa
     5881 ggtgtaggtg gtcgttgccg ggccggtgat ggtgtcgccc ttgaatatgc tctggctcgc
     5941 agaggagtcg gtgtagaccg cgaagttgtg cgggatgccc gagtcctggt tgtcgaagtt
     6001 caccgttact tccgcccccg ccggcaccgt gatggtgctc ttgtcgaagg cgatgttctg
     6061 ggcggtgagg tcaacagtcg cagtcccgcc gccagccgca gtggtcgctc ctgttgtggc
     6121 gtttgttgcc gctgtggttg tggtcgccgc cgtcgtctct gtagtcgtcg gtgtctcggc
     6181 ggcggttgtc gccgtagccg ccggtgtctc agcggtggtt gtccctgtag ccgctggtgt
     6241 ctcagcgacg gtcgtcgctg tgactgctgg tgtctcggtc ccggtcgggg gagccgtcgg
     6301 ctgggtgaca ttctcacccg gcgtggtgca ccccgcggcc agaactccgg cgctcacgac
     6361 aaggagcgct acgatgagga ttacgttagg cctcatggga gagaccataa cgcacccgat
     6421 atttaaatat tgccctcccc cgggctcacc cgtgccggag gcagagcggg tcgccgctgt
     6481 tgagatcgcc gtatcggacg taggcccgga cccggcaccc ggtaccgcag acctcctgcc
     6541 ggtcgcaggt gctgcacggg ctcccgggac gtgcctcgcc ctcccggaac gccgtaagca
     6601 cctggttgcc ggggtcgctc catatctcgc taaacggccg gtctcgtatg ttgcccgcga
     6661 gaaactcccg gaattgcgca aactggcagg ggtagacgtt cccggagggg tcgatattcg
     6721 ccacccgcct cccggcgctg catccgccca tgcgggcaag gaggcggtat gtccggtcgt
     6781 atgccggatg cgcatccgct ttcagcatct caagcaggca gacaccgtcc tggggagcat
     6841 cgacggtcag gaactccatc gccgccggat cggtcctgag ggtctcgcgg tagatcagat
     6901 cgatgactcc ctgcacctcc cctggcccga cctgcaggtc ggcgtgtgca tcgccccccc
     6961 gcccgctcgg gacgagccag tagacgcaga accggtgcac cccgatcgac cgggcaagcg
     7021 cgatcaggcg cccgagttca ctgtggttcc tcttcgtgac cgtgaaccgg acgccgcacc
     7081 ggaggcccgc atcgatgcag tacttcagcg cctgcaccga acggtcgaaa cttcccacca
     7141 ctccccggaa caggtcgtgg gttgccgccg tcgcgccgtc gagggatata ccggcatacc
     7201 caatcccggc atcccggagc cttcgggcga cggttggtgt gatcagtgtc ccgttggtgc
     7261 tcaggaccgc ggggatgccg cgctcttgag cgtgcgccgc gaggctccag aagtcttccc
     7321 gcatcagcgg ttccccgccg gagaagagga ggagcgggac gttcgccgca gcaaggtcgt
     7381 cgatgagaga ttttgcctcg cctgtcgtca gttcatcctc ccgccgccgg cggggcccgg
     7441 accggaggta gcagtgcgta caggcgaggt tgcaccgcga tgtgatgttc cagaagacca
     7501 ccggcccgcg gcgcccctcc tcaaacgcaa ggtggctatg cttcgccgcc gggccgctga
     7561 ccggcccttc cccgtgtatg aggtgcgtga accggatcac tcatctcccc tcctttgcga
     7621 ggagcgctaa ccccgggggt gtgcgtttca gctcccgggt gctgacgagg actgcgcagt
     7681 catcgatgcc ggccgcccgg gagaggcggg caatggagcg gtcgaccccg gcgcgacgcc
     7741 ggccgtgcag gacggcgtag aggttgtaat cccaccgccc gggaacagtg gctcgttcgt
     7801 agcagtgcgt cacctccggg gaccgggcca tgatctctgc tacctgcatc atatccccgg
     7861 gcggcacccg ccatacgacc atcgcgttga cccggatgcc ggctctccgg tggtcaagcc
     7921 gcaccgcgaa ccgccggatg atgccctgcg agagaaggga ctcgatccgg gcgatcacct
     7981 ccgcctccgt cacgtcgagt tcctccgcga tcgcccggta gggctcctcc accagcggga
     8041 cgcccgagag aagcctgatg aggagggtgc ggtcgttcat atgccgccct ccccgtcgcc
     8101 gtccgggaag aaccggaacc ggacgcctat cttgaaccgc cgcaccgccg gcaggtcgag
     8161 gagatcggcc ggggggacgc cggcccgggc ggcgatatct tccaccgtcc ggcgcagggc
     8221 ggcgctgtcc ggcgcggcga gcgtgaacca gaggttgtag tcgtggtcac ggaggtagtt
     8281 gtgcgagaca ccgggttccc ggttgacgat cgatgcgcac cgctccaccc ggtccgccgg
     8341 cacccgcatg ccgatgagcg tggaggccac ggggccgatc cgcgccgact cgatgaccgg
     8401 cgagacggtc aggacgatac cttcatcggc gagacggcgc acccgccgga gcacctcgcc
     8461 ctccggtatg ccaagacgcc tcccgatcgc gtgccacggc cgcggtgcca gggggaaggt
     8521 atcctgcagt atctcgagga gttgtcggtc aaggatgtcc aggtgccggt cagtcatgcg
     8581 ttatctctcc gggttcgtag gggcagcagg ggtcttccgc cagtatatcg ccgtccgggt
     8641 cgccggggcg caccgggccg ccgcaggcat cgccgcgccg gtgcctgacc ccgtaagccc
     8701 gtgcacggca cccgccgcag gcccggcggt actcgcattg cccgcacttc cccccgaggc
     8761 tgtcggggtc gcggagggcg gcgaagatct cggagccgtg ccagatatcg ctgaaagcct
     8821 ctcttcggat gtttccaacc gggacgggca ggtatgggca gggcgtcaca tctccgaccg
     8881 gggtgatccg gcagtatgcg gtgccggcga tgcatcccct gccccactgc gagaggtcaa
     8941 gccccatccg ggcggcgacc cggacaaact gcggggcgca tgtcggccgg accgagaacc
     9001 ccggttcccg ggcgtgggcg aggatgcgcc cgataaccgc ctcgtgaacc tccgggggga
     9061 ggtcggcgag gtcctctccc cggcccgtcg ggacagggaa gaagaactgg tagtcgcgga
     9121 cgccgtgact cttcccaaac gcgatgatcc tgtcgatctc gccgatgtca ggttcaccga
     9181 tcgtcgtgtg gatctggacc ggaacccccg ccctccggag agcccggatg ccgttgacgg
     9241 ccttctccca ggcgccactg acgcccctga aggcgtcgtg gacccccggg tccgtggagt
     9301 cgatgctgac cgccaccttc cggatgccgg tatcgctcag ccgttctgca acggcatcgg
     9361 tgacgaggat gccgttcgtg cccatggcga cgcgaagccc gcgcccggcg gcataccggg
     9421 caaggtcgaa gatgtcgggg cggaggagcg gttcaccccc gctgaggacc aggatcggcc
     9481 ggccgacggc ggcgatctgg tcgatcaggg ccatgccttc ggcggtgtcg agttcgccgg
     9541 gggagccctc gccgtccgcg tcgatgtagc agtgggcgca ggcgaggttg caccgggccg
     9601 tgacgttcca ggagacgatg cgcggtccgc cgccggcatc caggactctc tccatcaccc
     9661 tgcccccccc tccctgtgcg agaagagggc gagaagtccg gcaaagagcg cgatcaggtc
     9721 gaagggcggg aggatgcttg cgacgagggc ataccgcccg gccttccgga ggtcgccccg
     9781 ggcgatcgtc aggtatgccg caagaccaaa cacggttccg gcggccttga ctgcggcaag
     9841 gagaagcgcc cccgtcgtgg atatgccgga ggggccggcc tttcccgcaa tgccgcccgt
     9901 gccgataaca aggagcgtca ccaccaccag gaacccgaag accaccgata cggtgatgag
     9961 gattcgggcg atcttcacct cttttttcgt gcctaccatg ccgggcagtt cccctgtcgt
    10021 gaccattaac ctttccccgg ccggttgagt gatcgggatg gttgttcgtt accaaaaacc
    10081 ccggcctgaa gggaggagag atggggcagt tagccccttt ttcacatgcc gaccatcact
    10141 ctgcgataaa tttcccgttc atgaaggggt gaatgtcgca ctggaagtag taggtcccgg
    10201 gatcggatgg tgccgtaaac gtgtagttcg cctcggccgg cccggtgatg atctcgccta
    10261 cgaagaagga tttggagcgg aggttgctgt cgtagaccgc gaagttgtgc tggacgcccg
    10321 agtccttgtt cgtgaagtgc acggtcacgt tcgccccggc gggtaccgtg atggtgcttt
    10381 tgtcaaaggc gatgttctgg gcggtgaggt tcaccaccgt
//
LOCUS       DAOSII010000067        10375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_6287_length_10375_cov_53.276938, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000067 DAOSII010000000
VERSION     DAOSII010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10375
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6287_length_10375_cov_53.276938"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..2430
                     /locus_tag="PLV90_06325"
     CDS             166..2430
                     /locus_tag="PLV90_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF96769.1"
                     /translation="MSGDITEALKAMGDVDVASFPPGVVEYLREFKTQEIRDIVETLL
                     YIYIAQNASPPRGEGTGVVEQSCYAYTSGPLFVLAEVGLIESSSRHATTLVRVTPAGE
                     AIARPLAEARIQALDLGAIARETHEIVPVLLAGTVRGSYVNKTFPSSLPRTEETFIGF
                     LLNNNPALFEECERFAARLRAAGCAVLAHACDPDGAGTGSVVYAFPPEFAYILKRMLE
                     AIDPGIREHYDMLLESFHSTFTVLRYLACGEDYDRIRASAAHRRELSRMLSLIADSIY
                     ILEEVREDDTVGLARFIVKDRDRYDAYLRDLARALMADVAQKIVIDRPAPAADPRPPV
                     EEATPAPVREDVIAALPPADDEREEEWDDAIFTDEDEEEPLAPPPPPAPRRIVAPRPA
                     PEPPRPGDLDIFLGHAQDGHRVDWSPGRLNNGHMIILGGSGAGKTETIRCIATELAAQ
                     AMPVVLIDFHGDMAATTGDIRSYKIREGGEYYFNPLELDPAIDEITPLRATSDFVDAI
                     SINFPTLGIQQRRKIKNIIKDCYRASGITGNTGTWERVLDFDDIENEIMSCEDEAIPA
                     YLEDIFDYKLFSGEEKISIPTILSGGITHINLNALPENLRYLFADLFLRRIYYTLQAT
                     GEIPRGAKNEREKFRLFVIVDEAKLLVSQKSGSKTAIKAVLNKYATEMRKFGVSLILA
                     SQLIAHFNEEILANIAVKFCMRAENKKQAQENAKFFEVSEKDLLNFQPGEGILIIGSE
                     KMNVRIVPASGRKV"
     gene            2470..3057
                     /locus_tag="PLV90_06330"
     CDS             2470..3057
                     /locus_tag="PLV90_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HOF96770.1"
                     /translation="MDALDDYRGKRDFSRTPEPPGEGTGARDRPIFVIQMHDATRLHY
                     DFRLEADGVLKSWAVPKGPSVNPKERRLAVPTEDHPIGYADFEGVIPAGSYGAGTVLI
                     WDRGTYKNLTQENGQEIGVTGAIGRGHASIWLEGEKLRGGYALTRFRTGKGEAWLLVK
                     MDDAEADPGRDPVATEPRSVASGRTLAEIAAGVGE"
     gene            3683..4129
                     /locus_tag="PLV90_06335"
     CDS             3683..4129
                     /locus_tag="PLV90_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HOF96771.1"
                     /translation="MASKVMTNSAVANSVMAEAVSDAEIVAQFRQRGCWGLYTSVDLK
                     GCDPASIRDAEKIHRFIIELCDLIDMKRFGEPQIIHFGPNERVAGFSMTQLIETSLVS
                     GHFANETNAAYLDIFSCKEYEPSKAAEFCKTFFGAETATYQVLFRD"
     gene            complement(4341..5228)
                     /gene="frhB"
                     /locus_tag="PLV90_06340"
     CDS             complement(4341..5228)
                     /gene="frhB"
                     /locus_tag="PLV90_06340"
                     /EC_number="1.12.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase subunit beta"
                     /protein_id="HOF96772.1"
                     /translation="MDVLGNYKSVIAARSTDKDILKKAQDGGIITTLFAYALEEGIID
                     GAIVAGPGNEPWKPEPMVVSTKAELLAAAGTRYTISPNMSLIKEATRSYGLDRVGIVG
                     TPCQIQATRKAQLYPIGMRDVDDKIALALGIFCMENLSYQALEAIVEDHCNQKMESVR
                     KMDIGKGKFTVYTERGAVSQMPLKLIHKYVQPGCNVCLDYVANLADISSGSVGSPDGW
                     STVFVRSTKGNAVWDGAMKAGYFETKPMDQVKPGLDIVKKLATEKITKNQKNVDARKT
                     AGLKADGTPKGLRNPYVSP"
     gene            complement(5239..6000)
                     /gene="frhG"
                     /locus_tag="PLV90_06345"
     CDS             complement(5239..6000)
                     /gene="frhG"
                     /locus_tag="PLV90_06345"
                     /EC_number="1.12.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase subunit gamma"
                     /protein_id="HOF96773.1"
                     /translation="MAEKITIGELHLSGCTGCLVTLADNYEGLFKLLDNYADLVYALT
                     LVDVRHVPEMDVCLVEGSCCLNDKISVEELKQARAKSKVLVAYGGCAAYGNITRFCRG
                     GQWNQPGQEAFVPISEVVDVDLYLPSCPPCPQAVRNVAVMAYLLLKGNDEQKKLATAY
                     LTPLMQLAQRGNEACGCDLMYDVINQGLCMGCGTCAGTCPVRAITMEYGRPNVNRDLC
                     IKCGACYAQCPRSWFNFDVMNNYEGIMDAIKGAMQ"
     gene            complement(6140..6700)
                     /gene="frhD"
                     /locus_tag="PLV90_06350"
     CDS             complement(6140..6700)
                     /gene="frhD"
                     /locus_tag="PLV90_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-reducing hydrogenase, FrhD
                     protein"
                     /protein_id="HOF96774.1"
                     /translation="MLFREIVIAGCGNPLYGDDGFGPAVVEELQKLQLPDNVKVIDAG
                     LGAPHFLFTLMEDAEVPVKKLIIIDIADFGADPGDVTKLRPEDLPPGAYRDAHSWDLS
                     EPLQRLKDVIDITIIGCQPKRVASHEFELGLTDEVEGAIPRTVRIVLEEIGVEYGAAI
                     NHQGTHLWASPGETSGEAGDKTGEQT"
     gene            complement(6711..8078)
                     /gene="frhA"
                     /locus_tag="PLV90_06355"
     CDS             complement(6711..8078)
                     /gene="frhA"
                     /locus_tag="PLV90_06355"
                     /EC_number="1.12.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase subunit alpha"
                     /protein_id="HOF96775.1"
                     /translation="MSKVVEISPTTRHEGHSKLVLKVNDDGIVERGDWLSITPVRGVE
                     KLAIGKTMEQVPKIASRVCGICPIAHTLAGVEAMEASIGCEIPEDAKLLRYILQCANR
                     MHSHAIHNILSLPDMYIPGTDVKINPFTKEEPVRSVALRIQRLREIGQTIGEIVGGEA
                     IHPSNPRVGGMYKNITPRAKAKIYDLAKEGRVLAQEHMEFMVAVFKNFQNRDWSEVGG
                     VQVPIPDDLGYHNQGYMATAPVYGSSSLDENPTWFPERFTEVRPWDWYMGEVEVSEAD
                     PNYPIGGTSPVGTKAWPQMEACTGVPLYDGQPVEVGPRARLVQFRNYDGRGAMGLQIA
                     RQMEFPETAYGIIDALDALDTSGSVLADNIPQGDGSLGWAANEAPRGTDVHLARVKDG
                     RVQYFSMLVPTTWNFPTCSRALTGAPWRLAEVIMRAYDPCVSCATHMLVIDEDKRLVA
                     QKLIQ"
     gene            complement(8304..9245)
                     /gene="mptA"
                     /locus_tag="PLV90_06360"
     CDS             complement(8304..9245)
                     /gene="mptA"
                     /locus_tag="PLV90_06360"
                     /EC_number="3.5.4.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase MptA"
                     /protein_id="HOF96776.1"
                     /translation="MELPDVQSSLPEVRINLTRVGVKNVQKLVEVARPGKRPVIFISN
                     FDVFVDLPGSLKGANLSRNFEVIDDVLQQAIDGEVKVIEELCSAVARKLLDRHEYAER
                     TEVRMQSQFMVRRETPISETSCHEVVNVHASAIAQRNDGSPIIRKSIGAEVTGMTACP
                     CAQNIMKDHALHVLENLGVADDTINAFFEEVPMATHNQRGRGFLCIETDDDQHVSLEK
                     IIKILKDSMSARIYELLKRGDESYVVMEAHKNPRFVEDCVREMARKVITQFRDLPGDS
                     VVTIKQTNEESIHQHNAYAERKATIAELVGEMDEGAL"
     gene            9373..10362
                     /gene="ilvC"
                     /locus_tag="PLV90_06365"
     CDS             9373..10362
                     /gene="ilvC"
                     /locus_tag="PLV90_06365"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOF96777.1"
                     /translation="MVQKYYESDADPRTLEGRTIAVIGYGSQGRGQALNLRDSGCQVV
                     IGLRPGSSWQKASEDGFDVYSVADAVRRADVVQILLPDENQAAVYRAEIGPYLKENAC
                     LMFSHGFNIHYGQIVPPPTVDVIMVAPKGPGHMVRRTYEEGKGVPALIAIHQDASGKA
                     RAIALAYARGIGATRAVVLETTFAEETETDLFGEQAVLCGGVTSLIKAGFDTLVDAGY
                     APEMAYLEVLHEMKLIVDLIYEGGFTKMRDSISNTAQYGDLTRGPRVVGPETYAAMQE
                     ILEEIRNGEFAREWMLENMVNRPVFNALTRADEEHLIEQVGKEIRSLMPQFKK"
BASE COUNT         2067 a         3128 c         3047 g         2133 t
ORIGIN      
        1 gatattcggg ttttagcccc gaaatgtgct aaaaactgcc tatgtttaat acctcacacg
       61 ccaaacctat ctcaaccacc cgctttactg aagcggtaac cgcaagaccc acgccgcgcc
      121 ggtatccgga acatgccggc gaagaacgca tccgctggtg aacccgtgtc aggagatatt
      181 accgaagcac tgaaagcgat gggcgatgta gatgtagcgt cgtttccccc cggcgtcgtc
      241 gaatacctgc gagagttcaa gacgcaggag atccgcgaca tcgtcgagac gctcctctac
      301 atctacatcg cccagaacgc ctcgccgccc cgcggagagg ggacgggcgt ggtcgagcag
      361 agttgttacg cgtatacgtc cggaccgctc ttcgtgctcg cagaggtcgg tctcatcgag
      421 tcctcctccc ggcatgccac gacgctggta cgggtgaccc ctgccggaga agcgatcgcc
      481 cggccgctcg cggaggcccg tatccaggct ctggatcttg gggcgatcgc ccgggagacc
      541 cacgagatcg tcccggtcct cctcgcggga acggtgaggg gatcgtatgt caacaagacc
      601 ttcccctcat cgctcccccg caccgaggag accttcatcg gtttcctcct caacaacaac
      661 cccgcgctct ttgaagagtg cgagcggttt gccgcccgcc tcagggccgc cgggtgtgct
      721 gtcctcgccc acgcctgcga ccccgacgga gcgggaaccg gcagtgtcgt ctacgccttc
      781 cccccggagt ttgcgtacat cctgaaacgg atgctcgagg cgatcgaccc cggtatccgg
      841 gagcactacg atatgctcct tgagtcgttc cactcgacgt tcaccgttct gcgttacctc
      901 gcctgcggcg aggactacga ccggatccgg gcctcggcgg cccaccgccg cgagctgtcg
      961 cggatgctct ccctcatcgc cgactcgatc tacatactcg aggaggtgcg tgaggacgac
     1021 accgtcggcc ttgcccggtt catcgtcaaa gaccgggacc gctacgacgc ctaccttcgc
     1081 gacctcgcgc gggctctgat ggccgacgtc gctcaaaaga tcgtcatcga tcggcctgct
     1141 ccggcggcag atccccggcc tcccgtagaa gaggccaccc ccgccccggt gagagaagat
     1201 gttatagcgg ccttgcctcc agctgacgat gagcgggagg aggagtggga cgacgccatc
     1261 ttcaccgacg aggatgaaga ggaaccgctc gcaccacccc ctccgcccgc cccccgccgg
     1321 attgtcgccc ctcgccccgc cccggagccc ccccgccccg gcgatctcga catattcctc
     1381 ggccatgcac aggacgggca ccgggtcgac tggtcgccgg gcaggctcaa caacggtcac
     1441 atgatcatcc tcgggggttc gggagccggg aagaccgaga cgatccggtg tattgcaaca
     1501 gaacttgcgg cacaggcgat gccggtcgtc ctgatcgact tccacggaga tatggctgca
     1561 accacgggag atatccggtc ctacaagatc cgcgagggcg gcgagtacta cttcaacccg
     1621 ctggaactcg acccggctat cgacgagatc acccctctcc gggccacatc ggacttcgtc
     1681 gacgccatat ccatcaactt cccgacgctc ggcatccagc agcggagaaa gatcaagaac
     1741 atcataaaag actgctaccg ggcctcaggg atcacgggaa acaccgggac ctgggaacgg
     1801 gtgctcgact tcgacgacat cgaaaacgag atcatgtcct gcgaggatga ggcgatcccg
     1861 gcatacctcg aggatatatt cgactacaaa ctcttctcgg gagaggagaa gatatccatc
     1921 cccactatcc tctcgggcgg gatcacccac atcaacttaa acgcccttcc cgagaacctg
     1981 cgctacctct ttgcggatct cttcctccgg cgcatttact atacccttca ggcgacgggc
     2041 gagataccgc gcggggcgaa gaacgagcgg gagaagtttc ggctcttcgt gatcgtcgac
     2101 gaggcgaaac tcctggtgag ccagaagagc ggttcgaaga cggcgatcaa ggcggtgctg
     2161 aacaagtacg ctaccgagat gcggaagttt ggggtctcgc tgatccttgc atcgcagctg
     2221 atcgcccact tcaacgagga gatcctcgca aacatcgccg tcaagttctg tatgcgggcc
     2281 gagaacaaga agcaggctca ggagaacgcc aagttctttg aggtgagcga gaaggatctc
     2341 ctcaacttcc agcccgggga ggggatcctg atcatcggtt cggagaagat gaacgtcagg
     2401 attgttcccg cgtccggacg gaaggtgtag gcccaaaacc cttatccatg gcgccgccgg
     2461 tgttcatgta tggatgcgct tgacgattac cggggaaaga gggacttctc ccggacgccg
     2521 gagccgccgg gagaggggac gggtgcaaga gatcgcccca tcttcgtcat ccagatgcac
     2581 gatgcgacca ggcttcacta cgacttccgc ctggaggccg acggcgtcct gaagtcctgg
     2641 gccgtcccga aaggaccgtc cgtgaaccca aaggagaggc gccttgccgt ccccaccgaa
     2701 gaccacccga tcggttacgc cgacttcgag ggcgtcatcc cggcgggaag ttacggggcg
     2761 gggacggtcc tcatctggga ccggggaact tacaaaaacc tcacgcaaga gaacggacag
     2821 gagatcgggg ttaccggggc gatcgggcgg ggccatgcat cgatctggct cgaaggggag
     2881 aagctccgcg gcgggtacgc cctcacccgc ttcaggaccg ggaaaggtga ggcctggctc
     2941 ctcgtgaaga tggatgacgc agaagcggat cccggccggg atccggtcgc gacggaaccc
     3001 cggtcggtcg catccgggcg gacacttgcg gagatcgcgg caggggtggg tgagtaggcc
     3061 tgcgggacga tatcccggcc cggatatctt cgcgcgcgca ttaaggggct ccgatcgccc
     3121 ggagtgaggc ggggtgtgcg gcaccccccg tccccgcctc ccggcggtgt gcgtgatggc
     3181 ggagtcggga ggtgcgcggt gggaagtgga ggtatttgat ttccggaccg ggaaggatgg
     3241 ccccgtagtc actgcccggt tgccttccgg ccgtggtact ggccggtgca ggtgcaaaag
     3301 atcataaatc cacatatata ggtttttcat accaagaata aatcccgcgg ccgtaccgtg
     3361 aaaagggatt tttctcataa tcccgatcgc cctccggtcc ccgtagcggg gaccgcctga
     3421 aaattttgat tccggaataa catcacataa agtgctacat tcagtagatt gtttacacac
     3481 ccaaagacaa ataaacgacg caaagtcacc attttgtgaa taccgattga atatggtgta
     3541 gaatctttga tagaaagaga ttgctcggag gtaacgagga aataacggct atcctccatt
     3601 agtacgaaag ataagtttaa aatctgaact atcaaaaatt acagggatca gatcgatcac
     3661 gagatcttct cgaaggtgat tcatggcaag taaagtaatg actaacagcg ctgtggcaaa
     3721 cagcgttatg gcagaggcag taagcgacgc agagatcgtt gcgcagttca ggcaacgcgg
     3781 gtgctgggga ctgtacacga gcgtggacct gaaaggatgc gacccggcat cgatcaggga
     3841 tgcggagaag attcaccggt tcatcatcga actatgtgac ctcatcgata tgaagaggtt
     3901 cggcgaaccg cagatcatcc acttcggccc gaacgagagg gtcgcagggt tctccatgac
     3961 ccagctcatc gagacatcgc tggtctcggg gcacttcgca aacgagacca acgccgccta
     4021 ccttgacatc ttcagctgca aggaatacga accctcgaag gcggcggagt tctgcaagac
     4081 cttttttgga gcagagacag cgacctacca ggtactgttc agggactgaa tacgaataag
     4141 cacccggcat atcctgacga tacgctataa cggcagagag ctactgccat ctcccgccgg
     4201 cgtaagccag aggtcgcggc gaggtggcca ctgccctcga ttttttcaaa acttcgctct
     4261 ggagcagcgt ttccggtagt ggtcctgcca tccgcctttc tctcgagcgg agcagtcagg
     4321 gcgatcgaaa aaagagagtt tcagggagac acgtaggggt tccggaggcc cttcggggtt
     4381 ccatccgcct tgagacctgc ggtcttacgt gcatccacgt tcttctggtt cttcgtgatc
     4441 ttctcggtgg cgagcttctt cacgatatcg aggccgggtt tgacctggtc catcggtttc
     4501 gtctcaaagt agcctgcctt catggcaccg tcccagacgg cattgccctt cgtgctccgc
     4561 acaaagaccg tgctccagcc gtcaggactg cccacggacc cgctggatat gtcggcgagg
     4621 tttgcgacgt agtccaggca gacgttgcag cccggctgca cgtacttgtg gatcaacttc
     4681 agaggcatct gggagactgc accgcgttcg gtgtagaccg tgaacttgcc cttgccgata
     4741 tccatcttcc tgacggactc catcttctgg ttgcagtggt cctcgacgat cgcctcgagc
     4801 gcctggtagg aaaggttctc catgcagaag atgccgagcg cgagggcgat cttgtcgtcg
     4861 acgtcgcgca tgccgatcgg atagagttga gccttccggg tcgcctggat ctggcacggg
     4921 gtgccgacga taccgacgcg gtcaagaccg tagctccggg tcgcctcctt gatcagggac
     4981 atgttcgggc tgatggtgta gcgcgttccg gcagcggcca ggagttcggc cttcgtcgac
     5041 acaaccatgg gctcaggctt ccagggctcg ttgcccgggc ctgcgacgat ggcaccgtcg
     5101 atgatgcctt cctcgagcgc ataggcaaag agcgtcgtga tgataccgcc gtcctgggcc
     5161 ttcttcagga tatccttatc cgtcgagcgt gccgcgatca cggatttgta gttaccgagt
     5221 acgtccattg ttctcacctc actgcatggc tcccttgatg gcatccatga tgccctcgta
     5281 gttgttcatg acgtcgaagt tgaaccagct cctggggcac tgcgcgtagc aggcaccgca
     5341 cttgatgcag aggtcgcggt tcacgttcgg tctgccgtac tccatcgtaa tggcgcgaac
     5401 cgggcaggtt ccggcacagg ttccgcaccc catgcagagc ccctggttga tgacatcgta
     5461 catcaggtcg cagccgcagg cctcgttgcc gcgctgtgca agctgcatca gcggggtcag
     5521 gtaggcggtc gcgagtttct tctgctcgtc gttgcccttc agcagcaggt aggccatgac
     5581 ggcgacgttc ctgactgcct gggggcacgg gggacaggac ggcaggtaga gatcgacatc
     5641 gacgacctcg ctgatgggca caaacgcttc ctgtccgggc tggttccact ggccaccccg
     5701 gcagaaccgg gtgatgttgc cgtaggccgc gcaaccgccg taggcgacga gcaccttcga
     5761 cttcgccctt gcttgcttca gttcttctac cgagatctta tcgttcaggc agcacgaccc
     5821 ctcgaccagg cacacgtcca tctcggggac gtggcgtaca tcgaccaggg tcagtgcgta
     5881 gaccagatcc gcgtaattgt cgagcagctt gaagagaccc tcgtagttat ccgcaagcgt
     5941 cacgaggcat cccgtacatc cgctcaggtg taattcacct atcgtaatct tctctgccac
     6001 aggcttctcc tccttttgta caacttccac cttttctctt tcaatcacat cagagggctt
     6061 caccgcgggt ttcgcctgcg gccggctcgc ctcaggcttg ctctccggct tcacctcagg
     6121 cctgaccgcc ggtttcacct caggtttgct ctccggtttt atctccggct tctccgctgg
     6181 tttctcctgg cgacgcccaa agatgcgttc cttgatggtt gatagcagcc ccatattcta
     6241 ccccaatctc ctccagtacg atccgcactg tcctgggaat ggccccttca acctcatcag
     6301 tgagccccag ttcaaactca tggctcgcga cacgcttcgg ctggcacccg atgatcgtga
     6361 tatcgatgac atcctttaat cgctgcagcg gttcggaaag atcccacgaa tgggcatccc
     6421 ggtacgcgcc cggagggaga tcttcaggcc ggagtttcgt cacatcaccg gggtctgcgc
     6481 cgaagtcggc gatatcgatg atgatcagtt tcttcaccgg cacctccgca tcctccatca
     6541 gcgtgaagag gaagtgaggg gcgccaaggc cggcatcgat gaccttgacg ttgtcgggca
     6601 gctgcaactt ctgaagttcc tcaacaactg cagggccgaa cccgtcgtct ccgtagaggg
     6661 ggttgccgca ccctgcgatc acgatctcac ggaatagcat gcgtgtacgc tcactgaatg
     6721 agtttctggg ccactaacct cttgtcttca tcgatcacca gcatgtgcgt cgcacacgag
     6781 acgcaggggt cgtacgcacg catgatgact tccgcgagcc gccagggtgc gccggtcagc
     6841 gcacggctgc aggtcgggaa gttccaggtg gtcggaacga gcatcgagaa gtactggact
     6901 cggccgtcct tgacccgtgc caggtggacg tcggttccac ggggcgcttc gtttgctgcc
     6961 catccgaggg atccgtcacc ctgcgggatg ttgtccgcga gcaccgatcc ggaggtgtcg
     7021 agtgcgtcaa gcgcatcgat gatgccgtag gcggtctcgg ggaactccat ctggcgtgcg
     7081 atctgcaggc ccatggcgcc tctcccgtcg tagttcctga actggaccag gcgtgcacgc
     7141 ggtccgacct cgaccggctg gccgtcgtag agcgggacgc cggtgcaggc ctccatctgg
     7201 ggccaggctt tggtgcctac agggctcgtg ccgccgatcg ggtagttcgg gtcggcctcg
     7261 ctgacctcta cctcacccat gtaccagtcc caggggcgga cttcggtgaa ccgctcgggg
     7321 aaccaggtgg gattctcatc aaggctcgag ctgccgtaga ccggcgccgt cgccatgtag
     7381 ccctggttgt ggtagccgag atcgtcgggg atcgggacct gaacgccgcc gacctcggac
     7441 cagtcgcggt tctggaagtt cttgaagacc gcgaccatga actccatgtg ttcctgggca
     7501 aggacccgtc cttccttcgc gaggtcatag atcttggcct ttgcacgcgg ggtgatgttc
     7561 ttgtacatac cgccgacacg ggggttgctc gggtggatgg cctctccgcc gacgatctca
     7621 ccgatggtct gaccgatctc acggagccgc tggatccgca gagcgacgct cctgaccggt
     7681 tcctccttcg tgaacgggtt gatcttcacg tcggtcccgg ggatgtacat atccgggagg
     7741 gagagtatgt tgtggatggc gtggctgtgc atcctgttgg cacactgcag gatgtaacgc
     7801 agaagttttg catcctcggg aatctcgcac ccgatggatg cttccatcgc ctcaacgccc
     7861 gccagggtgt gcgcgatagg acatatacca cagacgcgcg atgctatctt tgggacctgc
     7921 tccatcgtct tgccgatggc gagcttctcg acacccctca ccggggtgat gctgagccag
     7981 tctccacgct cgacgatgcc gtcgtcgtta accttcagaa cgagcttcga gtggccttcg
     8041 tgtctcgttg ttggggaaat ctctacaact ttcgacaaat atatcgcctc attccgatat
     8101 tatggtgttc tgaacacgat acctgtgtta ccacacaact gtgtactgga aacgtacatg
     8161 ggtacgttag aagggaatat gaagaaaaaa gagtttataa ctttcgctcc agtacatccc
     8221 tatagattac ggatttagaa tttcgtagca attattacaa aaataggttg acaaattata
     8281 aaaatgtaag aataatatag gttttataat gccccctcat ccatctcccc gacgagttcg
     8341 gctattgtcg ccttccgctc cgcgtatgcg ttgtgctggt ggatgctctc ttcgttcgtc
     8401 tgttttatgg tgaccacgga atcgccgggg agatcacgga actgcgtgat caccttgcgg
     8461 gccatctcgc ggacacaatc ttcaacgaac cgggggttct tgtgagcttc catcaccacg
     8521 taactctcgt cgccgcgctt gaggagctcg tagatgcgcg cgctcatgga atccttcaag
     8581 atcttgatga tcttctcgag gctgacgtgc tggtcgtcgt cggtctcgat gcagaggaaa
     8641 ccccgcccgc gctggttgtg cgtggccatc ggcacctcct cgaagaacgc attgatcgta
     8701 tcatccgcca caccgaggtt ctcgaggaca tgcagggcat ggtccttcat gatgttctgg
     8761 gcgcaggggc aggcggtcat cccggtaact tcggcaccga tgctctttct gatgatcggg
     8821 cttccgtcgt ttctctgggc tatcgcgctt gcgtgaacgt tcaccacctc atggcagctc
     8881 gtctcgctga tcggcgtctc ccgccggacc atgaactggc tctgcatccg gacttcggtt
     8941 cgctccgcgt actcgtgccg gtcgaggagc ttcctcgcca ctgcgctgca gagctcctcg
     9001 atcaccttca cctccccgtc gatggcctgc tgcagcacat cgtcgatcac ctcaaagttc
     9061 cgggagaggt ttgcaccctt gaggcttccc ggcaggtcga cgaagacgtc gaaattggag
     9121 atgaaaatga ccggccgctt gccgggtcgg gcgacttcaa cgagtttttg aacgttcttc
     9181 acacccaccc tggtcaggtt gatacggacc tccggcaggc tggactggac atctggcagt
     9241 tccattacaa atcctcaata gtgtatagag aataaggtct ctcttcatca acattaaaat
     9301 aaccccctgc gtgagggcag ttttcccata taatatttat ctggtttgct gcccaaccgg
     9361 gagtgagaat atatggtcca gaaatactac gagtccgatg cggacccccg caccctggag
     9421 ggcaggacta tcgccgtcat cggctatggc tcccagggcc gcgggcaggc gctgaacctg
     9481 cgtgattccg gttgtcaggt cgtcatcggc ctgcggcccg gcagcagctg gcagaaggca
     9541 tccgaagatg gctttgatgt gtattccgtg gcggatgccg tcagacgcgc cgatgtcgtc
     9601 cagatccttc tccccgacga gaaccaggcg gccgtctacc gcgcggagat aggcccctac
     9661 ttaaaagaga acgcctgtct gatgttctcg cacgggttca acatccacta cggccagatc
     9721 gtccccccgc ccaccgtcga cgtgatcatg gtcgctccga aggggcccgg tcacatggtc
     9781 cggcggacct acgaggaagg gaagggagtc ccggccctca tcgccatcca ccaggatgcc
     9841 agcggcaaag cacgtgctat agcgctcgcc tacgcccggg ggatcggcgc gacccgtgcg
     9901 gtggtgctcg aaacgacgtt tgctgaggag accgagaccg atctcttcgg tgagcaggcg
     9961 gtcctctgcg gcggggtgac ctcgctcatc aaggcggggt tcgataccct ggtggacgcc
    10021 ggttacgcgc cggagatggc gtacctcgag gtcctgcacg agatgaaact catcgtcgac
    10081 ctgatctacg agggcgggtt tacgaagatg cgcgactcga tcagcaacac cgcccagtac
    10141 ggcgacctga cgcgcggccc ccgggtcgtc gggccggaga cctacgcggc gatgcaggag
    10201 atcctcgagg agatccggaa cggcgagttc gcgcgggagt ggatgcttga gaacatggtg
    10261 aaccgcccgg tcttcaatgc actgacaagg gcagacgagg agcacctgat cgagcaggtg
    10321 ggtaaagaga tccgttcgct catgccgcag ttcaaaaagt aacacccctt ttttt
//
LOCUS       DAOSII010000068        10343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_6307_length_10343_cov_46.056182, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000068 DAOSII010000000
VERSION     DAOSII010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10343
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6307_length_10343_cov_46.056182"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PLV90_06370"
     CDS             <1..207
                     /locus_tag="PLV90_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96778.1"
                     /translation="AFFMVLSRTLDLEVFFVLSLIGLLVIAVLIDGAFSRPRYMRHIG
                     YLIAAGVAIFAYIVAQKILEILAA"
     gene            204..1778
                     /locus_tag="PLV90_06375"
     CDS             204..1778
                     /locus_tag="PLV90_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOF96779.1"
                     /translation="MNHRASQIVLATAIIAALALLAGEATTPLLYTAANTSTAAHVDP
                     ATVPKQTGRDADALIPLMGDLLSQTGTLTLNIKLKDYESAERDLARYAELSGRFDRLV
                     INLDVSGTDIGEFQRNNRENLAALAALLNDTRRLEDLQRIEIDIQDDEGQRTTVIYEG
                     ETLQQKLQGGVTTYAGRENATIQIADRYGVNTTPYRESVRNYVEITGAVEAGGEKSPP
                     SPLGIAVAPDTGRYGDTFSIAGTYAGGSPGTPIEIYVDSRIAGNATLDENGAYTSIYR
                     IDRIPAGLHLAYAVAGAIYSGVTPFSVRQDDTAITLTLGEGEVCTGSLMTAGGRPVTL
                     APVLLRVDGSTPIETRTDENGTYRASLALPAGEHAIRAEFNAAGYPLNASESPTETIV
                     VRNEGLSPLPFIAAIAAALGSGWYLRRRNRPEEKAPAAEPEESRETEATITPPQADIA
                     GLSPREAATVLFWALRSRLGLPDTNTPRDCARLIPAHAGFFERYEQIRYAGETPSEEE
                     LRAMKEEALGGEERAA"
     gene            1753..2580
                     /locus_tag="PLV90_06380"
     CDS             1753..2580
                     /locus_tag="PLV90_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HOF96780.1"
                     /translation="MEAKNVQREAWAVLLILFLAGAVVYAHATTTTEEYSRYNPGWNG
                     TSNLPAEEIRDLADLDPSTILLILAPDQPFSPDEVGSLRAFLDAGGRLLIADEEGNAN
                     PLLADLGSSMRVRPGNLSSLNRDHVNPGLFTVSVVGNDTLFAGVETARVNRPAAVEGG
                     EPLLETSPFTWDDADGDGRISGSETFGTAVVCAREGNLTVLGDASLFVNAMFAENPKF
                     IENLQPIRVDGIHSRTGTKSPIINTLVWIRTTPPAGAAIVALGVLPVAWIFGRKRDE"
     gene            2573..3544
                     /locus_tag="PLV90_06385"
     CDS             2573..3544
                     /locus_tag="PLV90_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOF96781.1"
                     /translation="MNESLNERIKTIADAYEDIRLITQQVVVANQPLIEEIFISMING
                     GHLLIEGVPGTAKTTVCKIIARLMDYEFRRVQGAVDLQPADIIGVRTYDRNKNKFTLQ
                     KGPIFTNFLMVDEMNRLTPKTQSALLEALSERQATIDGISYPLSDAYFVIATQNPYDS
                     EGTFPLIEAQRDRFMFSTVLNHLDPEGELEILRRERAGELDWKIYRDRVAPVLGPKEI
                     GAMAATIREIRVDETVLIYVRDLVSATRSHSDVRLGASSRASIALLGGSRVRAALDGR
                     TYVIPDDARALAVPALRHRILLTREAAIAGVTPDAVVGEIIESAGVS"
     gene            3547..4791
                     /locus_tag="PLV90_06390"
     CDS             3547..4791
                     /locus_tag="PLV90_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOF96782.1"
                     /translation="MLRPTRTAGGVAALALLLAAAAHLLLMPAAALAAGSLGLFLLWR
                     GWRFGNDLAVAVTSLTVTRDVDPTILRQGAVASVRVQADCAVPPGMAIHVRDIPPAVA
                     AGDGPLCEPGKAVTYAVRLMAPGETAFGGIVLSAGDAFFSCDLFCRRFDAPRLRVFPV
                     GTPEDGRGIGTHGESTEVDRKTTLAAQGVRGFRAYQKGDDPRLVDWKVTARRGTLYIR
                     EMTGLSGGAPLIAVDIPILTGDPEDFARLSTAVYGAIEGAIASRDGCSLLVAAGGEIV
                     RFLPRILANREAFAALGGLSPLEPRAHLYRAPGPAVLAGRARMSGQGAGPEERVFRER
                     LGGVLSAFAATSPAPFTVAIRAALDRAEATDIRIYSLLSEGDKSHLLQLIHEAKVRGM
                     RVTLRAPAGAGTLPGVDAVEVL"
     gene            4791..5084
                     /locus_tag="PLV90_06395"
     CDS             4791..5084
                     /locus_tag="PLV90_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96783.1"
                     /translation="MAVDRKFLLLCAAEILVVAVGDLFAALILQTIVLAAFLGDRRGY
                     PAYAGAIVLFAAVLLASGRILLPLLGLTAALGGGFLALTLRDYRLARWARGEA"
     gene            5081..5854
                     /locus_tag="PLV90_06400"
     CDS             5081..5854
                     /locus_tag="PLV90_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96784.1"
                     /translation="MTRSNPYRGAFSLILATVLLLAVAAVTGRGDITTATVVLAGTGC
                     FIAGIFLLALHKGEPLNPDVAALLPVQGTIGTATLCADLGVQGDARFVPEDGTVIEVI
                     PVAGTLPEKPNGDYSFITGSICAVRLVPACAPLLARLREKHALEVPEEDEALLACIAE
                     VCDDLIEVAEKTEAKRDGENIVVTLHGYRLIPGCRAVRAASPKCCTMVACPVCSLIAA
                     IVALGKKTPVRIEHTAVDERRGVLRVILTPAGNTGSNES"
     gene            5960..6298
                     /locus_tag="PLV90_06405"
     CDS             5960..6298
                     /locus_tag="PLV90_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOF96785.1"
                     /translation="MGRYVSGDVLLAAVRIGGGGEEKVRPVVVIAAGENGCLIVCPVS
                     SRPSFDGPSVPLSLDDFARGGLGLFTESYALAAHPSAIRSADIIGKKGTLLPEALAAI
                     RGAVPRKERF"
     gene            complement(6295..8013)
                     /locus_tag="PLV90_06410"
     CDS             complement(6295..8013)
                     /locus_tag="PLV90_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF96786.1"
                     /translation="MDSDLNRAPGGLPVPGALEDGLLVVDGDRKILWCNPALARMLGL
                     PPGEVPGTDLLSLLEEHLLPAIEDEASARSIRQTLLDGADLPDLCCRTRSPAGTRRWL
                     SIAGSRIPGGQHLIRIRDAARDIDAHHFRTALKQSPVVVFAQDLDLRYIWSCNQQLGP
                     TDESIIGARDEEIFPDGGAHFTAIKRWILETGEGMRTSMTLNVDGDHHVRDLTLKPLR
                     DAEGRIAGITGTAFDVTERDRAEKALQKRMSELQCLYAITHLVEVPGITPDGLLQAVA
                     DTLPSGWQYPDDAAVRIQIDDREYRRGEPKETPWRQASPIIVCGERRGEVEIWYTSEK
                     PEAVEGPFLTEERMLLDAVAERLGRIIERLQIEAALRESEAKFRGIAQRSFDLIATSY
                     LDGGLNYISPAMERILGYSPAEMKGTDWEDYILPVSLPVWEEGRQRMLQNRQVEGLQI
                     EVRQKNGETAVLELNGSPIVEKGMIVGFQAVGRDISERVLYERLREQTLDLNARNIAQ
                     FAILADHVRHPLQVVLGLADLLDDEKTAEILREQVRRINARISELDREWVESRKIREF
                     LKRYEL"
     gene            8227..8502
                     /locus_tag="PLV90_06415"
     CDS             8227..8502
                     /locus_tag="PLV90_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOF96787.1"
                     /translation="MQVRTRVLVAGRVQGVGFRWAVEDEARDAGVTGWVRNLPDGRVE
                     AVFEGEEESVRRMVGFCRRGPAAARVDDVAIIKEEYTGKFEGFFILS"
     gene            8555..9142
                     /locus_tag="PLV90_06420"
     CDS             8555..9142
                     /locus_tag="PLV90_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HOF96788.1"
                     /translation="MVTDTEAIGIFSVIRERRSVRDYVDRDLPDEALRAIIAAGVQAP
                     NALGLQPWRFVVVKDRSLMRRASDYCKPILAEKIGGEPRPGVENFLMALKDPEYSIFY
                     NAPALVLVLGAREVVSSAIDCALCAENMMLAAWAIGIGSCWVGSAALIQQNPDLLRAL
                     KVPDDHRIVAPLIFGYPAPLQPKPGRREPQITWIP"
     gene            complement(9145..10200)
                     /locus_tag="PLV90_06425"
     CDS             complement(9145..10200)
                     /locus_tag="PLV90_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOF96789.1"
                     /translation="MDKIKHKLFQQVSGSSPASVANGSSAPPDTGESDADLLDAYSRA
                     VVRVVETVGPAVVSVVVGKNPRDRRGEQIGAGSGVIVTPDGYIMTNNHVVQGAGRIEV
                     RTADGTALPARLAGADPATDLAVLRADAGGLPSAAFGDSGALSVGQLAIAIGNPLGFD
                     STVSTGVLSALGRALRSRDGRLIENVIQHTAPLNPGNSGGPLVDSRGRVIGINTAIIP
                     MAQSICFSIPANTAAWVLPQLVADGRVRRGYLGIAGQSRPLERSLVLALGLTGGHAVE
                     VVSVSRESPAGRAGLRPGDLIIAINETGVGCVDDLHRFLATWPIAEPATLTLIRGRQR
                     IRLPIIPIEAVACPAVG"
BASE COUNT         1746 a         3577 c         3255 g         1765 t
ORIGIN      
        1 gccttcttca tggtcctctc ccgcacgctc gaccttgagg tcttcttcgt cctctcgctc
       61 atcggcctcc tcgtgatcgc ggtcctgatc gacggggcct tctcccgccc ccgctacatg
      121 cgccacatcg gctacctcat cgcagcaggt gtcgccatct tcgcctacat cgtcgcccag
      181 aaaatactgg agatccttgc cgcatgaacc accgggcctc gcagatcgtc cttgcaaccg
      241 ccatcatcgc ggcgctcgcc ctccttgcag gagaggcgac caccccgctc ctctacacgg
      301 cggcaaacac atcgaccgcc gcccacgtcg accccgcgac cgttccgaaa cagaccggca
      361 gggatgccga tgccctcatc cccctgatgg gtgacctcct cagccagacc ggcacgctca
      421 ccctcaacat caagctcaag gactacgaga gcgccgagcg cgacctcgcc cggtatgccg
      481 aactctcggg ccggttcgac agactcgtca tcaatctgga cgtctccgga accgatatcg
      541 gcgagttcca gcggaacaac cgggaaaacc tcgctgctct cgcagctctc ttaaacgaca
      601 cccgacgact cgaggactta cagaggatcg agatcgacat ccaggacgat gaaggccagc
      661 gtacaaccgt catctacgag ggcgagacgc ttcaacagaa actgcagggt ggagttacca
      721 cctacgcggg ccgggagaac gcgacgatcc agatcgccga ccgctacggc gtaaatacca
      781 ccccctacag ggagagcgtc agaaactatg tggagatcac cggtgccgtg gaggcgggag
      841 gcgaaaagag ccctccatcc cccctcggca tcgcggtcgc ccccgatacg ggtcggtatg
      901 gagatacctt ctcgatcgcc gggacttacg ccggcggctc cccgggcacc cccatagaga
      961 tctacgtcga cagccggata gccgggaacg ccaccctgga tgagaacggc gcctacacct
     1021 ccatctacag gatcgaccgg atccccgccg gcctgcacct tgcctacgcc gtcgcgggtg
     1081 cgatctactc cggcgtcact ccgttctcgg tccgccagga tgataccgcc atcaccctca
     1141 ccctcgggga gggcgaagtc tgcacgggaa gcctcatgac cgccggaggc cggccggtca
     1201 cgctcgcccc ggtcctcctc cgggtcgacg gcagtacacc gatcgagacc aggaccgacg
     1261 agaacggcac ctaccgggca agcctcgccc tccccgccgg ggagcacgcc atccgggcgg
     1321 agttcaacgc cgcaggctac cccttaaacg cctctgagag cccgacagag acgatcgtcg
     1381 tccggaacga ggggctctcc cccctcccgt tcatagcggc aatcgcggcg gcgctcggga
     1441 gcgggtggta cctccggcgc cgaaaccggc cggaagagaa ggccccggcg gcagaacccg
     1501 aagagagcag ggagacagaa gccaccatca ctccgccgca ggcagatatc gcgggactct
     1561 ctccccgcga ggccgcgacc gttctctttt gggctctccg gtcccggctc ggtctccccg
     1621 acacgaacac cccgcgggac tgcgcccggc ttatccccgc tcacgccggg ttcttcgagc
     1681 gctacgagca gatccgctac gccggcgaga ctccatcgga ggaggagctc cgcgcaatga
     1741 aagaagaggc tcttggaggc gaagaacgtg cagcgtgagg cctgggcggt cctcctcatc
     1801 ctcttcctcg ccggcgccgt agtctacgcc cacgcgacca ccaccaccga ggagtacagc
     1861 cgctacaatc cgggctggaa cggcacctca aacctccccg cagaggagat ccgggatctc
     1921 gcggatctcg acccctcgac gatcctcctc atcctcgcgc cggatcagcc cttcagcccc
     1981 gacgaggtcg gctccctccg ggcgttcctc gatgccggcg gccgcctcct catcgccgac
     2041 gaggaaggga acgctaaccc gctcctcgcc gacctcggga gtagtatgag agtccggccg
     2101 gggaacctct caagtctcaa ccgcgaccac gtcaaccccg gcctcttcac cgtcagcgtc
     2161 gtcgggaacg acaccctctt tgccggggta gagaccgccc gggtcaaccg cccggccgcg
     2221 gtcgagggag gcgagcccct cctcgagacg tcgcccttca cctgggacga cgccgacggc
     2281 gacggccgga taagcggatc cgaaaccttc gggaccgcgg tcgtctgcgc ccgggaagga
     2341 aacctcaccg tgcttggcga cgcaagcctc ttcgtcaacg ccatgttcgc cgagaacccg
     2401 aagttcattg agaatctcca gccgatccgg gtagacggca tccacagcag gaccggcacg
     2461 aagagcccga tcatcaacac cctggtatgg atccggacaa ccccaccggc cggagccgcc
     2521 atagtcgccc tcggcgtcct gccggtcgcc tggatctttg ggaggaagag agatgaatga
     2581 gagtttaaat gaacgcatca aaacgatcgc ggatgcttat gaggatatcc ggctgatcac
     2641 ccagcaggtg gtcgtcgcaa accagcctct catcgaagag atcttcatca gcatgatcaa
     2701 cggcggccac ctcctcatag agggggtgcc gggaaccgca aagaccaccg tctgcaagat
     2761 catcgcacgc ctgatggatt acgagttccg gcgggtccag ggagccgtgg acctccagcc
     2821 agccgacatc atcggggtcc ggacctacga ccggaacaaa aacaagttca ctctccagaa
     2881 agggcccatc ttcacgaact ttttgatggt cgacgagatg aaccgcctga ccccgaagac
     2941 ccagagcgcc ctcctcgagg ccctgagcga gcggcaggca acgatcgacg gtatatccta
     3001 cccgctcagc gatgcctact tcgtcatagc cactcagaac ccctacgact ccgagggaac
     3061 cttccccctc atcgaggccc agcgcgaccg gttcatgttc agcaccgtcc tcaaccacct
     3121 cgacccggag ggagaactcg agatcctccg gcgggagcgg gccggggaac tggactggaa
     3181 gatctaccgg gaccgggtcg cccctgtcct cgggccgaag gagatcgggg cgatggccgc
     3241 gactattcgg gagatccggg tcgacgagac ggtcctcatc tacgtccgcg acctggtgtc
     3301 tgccacccgg tctcatagcg atgtccggct cggcgcgagt tcccgtgcat cgatcgccct
     3361 cctcggcggc tcgagggtcc gggcagccct ggacggccgg acctatgtca tccccgacga
     3421 cgcccgggcg cttgccgtac ccgctctccg gcaccgtatc ctcctcaccc gggaggcggc
     3481 gatcgccggg gtaaccccgg atgcggtcgt cggcgagatc atcgagagtg ccggggtgtc
     3541 gtaaggatgc ttcggccgac ccggaccgcc gggggagtcg cggcgcttgc tctcctcctc
     3601 gccgccgcag cccatctcct cctgatgccg gccgcggccc ttgccgccgg gtctctcggc
     3661 ctcttcctcc tctggcgggg gtggcggttc gggaacgatc tcgcagtcgc ggtcacttcc
     3721 cttaccgtga ctcgcgacgt cgacccgacg atcctccggc agggggcggt ggcgtccgtc
     3781 cgggtgcagg ccgactgcgc cgtcccgcca ggcatggcta tccacgtgcg tgatatcccg
     3841 ccggcagtcg cggccggcga cggcccgctc tgtgagccgg gaaaggccgt gacctacgcc
     3901 gtccggctca tggcgccggg agaaacggcg tttggcggga tcgtcctctc ggcaggcgat
     3961 gcattcttct cctgcgatct cttctgccgc agattcgacg ccccccgcct ccgggtcttt
     4021 cccgtgggga ccccggagga cgggaggggt atcgggacac atggagaaag taccgaagtg
     4081 gaccggaaga ccacgcttgc cgctcaaggc gtccgcgggt ttcgggctta ccagaagggc
     4141 gacgaccccc gcctggtgga ctggaaggtc accgcccggc gcggcaccct ctatatccgc
     4201 gagatgaccg gcctatcggg cggtgcgccc ctgatcgccg tcgacatccc gatcctgacc
     4261 ggagatccgg aagacttcgc ccggctctcg acggccgtct acggcgccat cgagggggcg
     4321 atcgcctccc gcgacggctg ctcgctcctg gtcgccgccg gcggagagat tgtccggttt
     4381 ctcccgcgta tcctggcgaa ccgtgaggct ttcgcggctc tcggcggcct ctcgcccctc
     4441 gagccccgtg cccacctcta ccgcgcaccg gggcccgccg tcctcgcggg acgggcgagg
     4501 atgagcgggc agggagcggg gccggaggaa cgggtattcc gcgagcggct cggcggagtc
     4561 ctctcggcgt tcgcggccac gagccccgca cccttcaccg tggcaatccg ggcggccctc
     4621 gaccgggcgg aggctacgga tatccggatc tactctctcc tttcggaggg cgataaaagc
     4681 catctcctcc agttgatcca cgaggcaaag gtccggggga tgcgggtcac cctccgggcc
     4741 cccgccggtg ccgggaccct ccccggcgtc gatgccgtgg aggtgctctg atggcggtcg
     4801 atcgaaagtt cctcctcctt tgcgcggccg agatcctggt cgtcgccgtc ggggatctct
     4861 tcgcggctct catcctccag acgatcgtcc tcgccgcgtt cctcggcgat cgacggggct
     4921 atcctgctta cgccggggcg atcgtcctct ttgccgcggt cctcctggca agcggccgga
     4981 tcctcctccc cctcctcggc ctcacggccg ctctcggcgg cggttttctt gccctcaccc
     5041 tccgggacta ccggctcgcc cggtgggcac gaggtgaggc gtgacgcgga gcaaccccta
     5101 ccggggagcg ttctccctca tcctcgctac ggtcctcctc ctcgccgtcg cggccgtgac
     5161 cggcagagga gacatcacca ccgcaacggt cgtcctcgcg ggcaccggct gcttcatcgc
     5221 cggcatcttc ctcctcgccc tccacaaggg cgagcctctc aatccggatg tcgccgccct
     5281 cctccccgtc caggggacga tcgggaccgc cacgctctgc gcggacctcg gcgtccaggg
     5341 cgatgcccgg ttcgtcccgg aggacgggac cgtcatcgag gtgatcccgg tcgcaggcac
     5401 cctcccggag aaacccaatg gcgactactc cttcatcacc ggcagtatct gcgccgtccg
     5461 gctcgttccg gcatgcgccc cgctcctcgc ccgtctccgc gagaagcacg ccctcgaggt
     5521 gccggaggag gacgaggccc tccttgcctg catcgccgag gtctgcgacg acctcatcga
     5581 ggttgccgaa aagacggagg cgaagagaga cggcgagaac atcgtcgtca cccttcacgg
     5641 ctaccggctc atacccggct gccgggctgt acgggcagca tcaccaaaat gctgcaccat
     5701 ggtcgcctgc ccggtctgca gcctcatcgc cgccatagtg gcgctcggga agaagacccc
     5761 ggtgaggata gagcataccg ccgtcgacga gaggaggggc gtactccggg tcatcctcac
     5821 gccggccggg aatacagggt ccaatgagtc gtgatccggc agttgccggg gaaggatctt
     5881 catgaggcgc ggttcttcct gacacgggag gggcacggac caatcctctt atcgaccctg
     5941 ggcgccaacc tctctgtcca tggggcggta cgtcagcggc gacgttctcc tggccgccgt
     6001 gcgcatcggg ggaggaggcg aagagaaggt ccggccggtg gtggtcatcg cggcaggaga
     6061 gaacgggtgc ctgatcgtct gcccggtctc aagcaggccc tccttcgacg gaccctcggt
     6121 cccgctctcg ctcgacgact ttgcccgggg cgggctcggc ctcttcaccg agagctacgc
     6181 tcttgccgca cacccgtccg ccatacgctc ggccgacatc atcggtaaga agggcacgct
     6241 cctgccggag gcgctcgccg cgatccgggg agccgtgccg cgaaaagaga ggttttagag
     6301 ttcgtagcgc ttcaggaact cccggatctt ccgcgactcc acccactcgc ggtcgagctc
     6361 gctgatccgt gcattgatcc tcctgacctg ttcacggaga atctctgcgg tcttctcatc
     6421 gtcaagcaga tccgcaagcc ccagcaccac ctggagcggg tggcggacgt ggtcggcaag
     6481 gatggcaaac tgcgctatgt ttcgtgcatt caggtcgagg gtctgctcac ggagccgttc
     6541 atacagaacc cgctcggata tatcccttcc tacggcctgg aaccccacga tcatcccttt
     6601 ctcgacgatc ggcgacccgt tcagttccag cacggcagtc tccccgttct tttgcctgac
     6661 ctcgatctgg agcccctcga cctgcctgtt ctggagcatt cgctggcgcc cctcttccca
     6721 taccgggagg cttaccggga ggatgtagtc ttcccagtcg gtccctttca tctccgcggg
     6781 cgagtagccg agtatgcgct ccatcgccgg cgatatgtaa tttaaccccc cgtcgaggta
     6841 ggacgtggcg atgaggtcga aactccgctg ggcgatcccc cggaactttg cctcgctctc
     6901 ccggagcgcc gcctctatct ggagccgctc gatgatccgg ccgagccgct ccgcaacggc
     6961 atcgagcagc atccgctcct ctgtgagaaa cggcccctcg acagcctccg gtttctcgct
     7021 ggtataccag atctcaactt cgccgcgccg ttcgccgcag acgatgatcg ggcttgcctg
     7081 tcgccagggc gtctctttcg gttcaccccg gcgatactcc cggtcatcga tctggatccg
     7141 cacagcggca tcgtccgggt actgccagcc tgaggggagg gtatcggcaa ccgcctgcag
     7201 gagcccatca ggcgttatcc ccggcacctc gacgaggtgc gtgatggcat acaggcactg
     7261 gagctcgctc atcctcttct ggagcgcctt ctccgcccga tctctctcgg ttacgtcgaa
     7321 ggccgtcccg gtgatgccgg cgatccggcc ttcggcatca cggaggggtt tgagtgtcag
     7381 gtcacggaca tggtggtcgc cgtcgacgtt gagcgtcatc gacgtccgca tcccttctcc
     7441 cgtctcgaga atccagcgct tgatcgcggt aaagtgcgcc ccaccgtcag gaaagatctc
     7501 ctcgtcccgg gctcctatga tggactcgtc cgtgggcccg agctgctggt tgcaggacca
     7561 gatatagcgc agatccaggt cctgcgcaaa gacgaccacc ggtgattgct tgagcgcggt
     7621 cctgaaatga tgcgcatcta tatccctggc ggcgtcccgg atcctgatga gatgctgccc
     7681 gccggggatc cgtgaccccg caatcgagag ccagcgccgg gtgccggcgg gcgaccgggt
     7741 gcggcagcaa agatcgggga gatccgcgcc gtcgaggagt gtctgccgga tcgaccgcgc
     7801 ggaggcctca tcctcgatgg ccggcaggag gtgctcctcg aggagggaga ggagatccgt
     7861 cccgggtacc tctcccggcg ggaggccgag catccgcgca agggccgggt tgcaccagag
     7921 gatcttccgg tcgccatcca ccacgagaag accgtcttcg agtgcgccgg gcaccggcag
     7981 gccgccgggt gcccggttca gatctgagtc cattgtttcc caccagcgct ggagtatagc
     8041 tgccttaggt gttggatctc gaagaactaa aatgagttac gatctgatgt tcggccggca
     8101 ttacctgcat actcatcttc gagggaatat tccggccggt tttaccacct cgaagggggt
     8161 gggggttccg gaaaaacccc ggcgtgcccg gcagggataa gtcttatcgc cgccgacccc
     8221 tggtatatgc aagtgcggac gcgcgtactg gtcgccgggc gggtacaggg tgtcggattc
     8281 cggtgggccg tggaggacga ggcccgggat gcaggcgtca ccgggtgggt caggaacctc
     8341 cccgacggcc gtgtggaggc ggtctttgag ggcgaggagg agtccgtgcg gcggatggtc
     8401 gggttctgcc gcaggggtcc ggccgcggcc cgggtcgacg acgtggcgat catcaaagag
     8461 gagtataccg ggaagttcga gggtttcttc atcctctcct gatagaggtc ggcccctcaa
     8521 ggcatatata cgatggcctt ccttcggagg acgtatggtg acggatacag aggcgatcgg
     8581 gatcttctcc gtgatccggg agcgccggag cgtcagggat tatgtcgacc gcgatctccc
     8641 ggacgaggcc ctccgggcga tcatcgctgc gggcgtccag gccccgaacg ctctcgggct
     8701 tcagccctgg cggttcgtgg tcgtgaagga taggagcctg atgcggcggg catccgacta
     8761 ctgcaaaccc atccttgcag agaagatcgg gggggagccc cggcccgggg tggagaactt
     8821 ccttatggcg ctcaaagatc cggagtacag catcttctac aacgccccgg ccctggtcct
     8881 ggtcctcggc gcccgtgagg tcgtatcgag cgccatcgac tgcgcgctct gcgcggagaa
     8941 catgatgctc gccgcctggg cgattggcat cggcagttgc tgggtcggct ccgcggccct
     9001 catccagcag aaccccgatc tcctcagggc gttgaaggtc ccggacgacc accggatcgt
     9061 ggcgcccctg atattcggct accctgcccc cctgcagccg aaaccgggac ggcgcgagcc
     9121 gcagattacg tggataccgt gacctcaccc gacggcgggg cacgcgacgg cctcgatcgg
     9181 tatgatcggg agccggatcc gctgccgtcc ccggatgagc gtcagggttg ccggttccgc
     9241 gatgggccag gtggcgagga accggtgcag gtcgtccacg caccctacgc cggtctcgtt
     9301 gatcgctatg atcaggtcgc ccggccggag ccctgccctg ccggcggggc tctcccgcga
     9361 gaccgagacg acctcgaccg catggccccc ggtaagcccg agagcgagga cgagcgaccg
     9421 ctccagcggt cgtgactggc cggcaatgcc caggtagccg cgccgcaccc ggccgtcggc
     9481 gacgagctgc gggaggaccc aggcggcggt gtttgcaggg atcgagaagc agatgctctg
     9541 ggccatgggg atgatggcgg tgttgatccc gatcacccgg ccgcgcgagt ccaccagcgg
     9601 gccgccggag ttgccggggt tcaggggcgc ggtgtgctgg atgacgttct cgatcaggcg
     9661 accgtcccgg ctccggagcg cccggccaag agcgctcagg acccccgtcg agacggtcga
     9721 gtcgaatccg agcgggttgc cgatggctat cgcaagctgg ccgaccgaga gcgccccgga
     9781 gtcgccgaac gctgcagagg ggagaccccc ggcatcggca cggaggacgg cgagatcggt
     9841 cgccggatcg gccccggcca ggcgtgcggg gagagcggtc ccatccgccg tacggacctc
     9901 gatcctgccc gcgccctgca ccacgtggtt gttggtcatg atgtagccgt ccggtgtcac
     9961 gatcacgccg gaaccggccc cgatctgctc gcccctccgg tcccgggggt tcttccccac
    10021 gaccacgctc acgactgcgg gccccacggt ctcgacgacc cgtacgacgg cccgggagta
    10081 ggcgtcaagg aggtcggcgt cggactcccc ggtatcaggg ggtgcagagg acccgtttgc
    10141 tacggatgcc ggagatgagc cgctaacctg ctgaaagagt ttgtgtttta ttttatccat
    10201 tacaggatat aatttgtaac caatagtaat gtactttctg cacagggttg agatagatcc
    10261 ctgccggcgg cgtgcggcac agccccgttc tccggcgcgc gagaactttc gcaggcgggt
    10321 taatcatgcg ttctgctccg gca
//
LOCUS       DAOSII010000069        10191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_6407_length_10191_cov_52.203729, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000069 DAOSII010000000
VERSION     DAOSII010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10191
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6407_length_10191_cov_52.203729"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..1563
                     /locus_tag="PLV90_06430"
     CDS             13..1563
                     /locus_tag="PLV90_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOF96790.1"
                     /translation="MPNCTTFLDANAANPAKTALIIPSTGESYTRADLLDTVARAAGG
                     LLALGVERGERVCIYLESSVEYLISYLALWRIGAVAVPTNRVYRESEVLYALRDAGAA
                     AVITDAEGAALVGRIRDRAPTLRHVVAVSGNIAGTTPWTDLLHAEPCRRAAHCRFDDL
                     CQIQYTSGTTGRPKGAMLTHGNWIAAMDAERDLLGITADDIYLGIYPMGHVGISWGIS
                     TLRAGGTYVVMERFDLSRYIDLAREYRATIVAGMPPVIHSLLQTPPGTEAALATVRVM
                     ISGGGPLMPGVWKPFHERFGIPVANAYGLSETVVVGTGTAIRPEHYATADEFRSVGTP
                     VGFSEVKIVDADDPAQELGPGEDGEIALRGPGVALGYWQMPEETRAAFLPDGWFLTGD
                     IGHLDADGMLAITDRKKDMIVMSGWKVYPTEVEDALIQHPGIRDAAVFGCPDERRGEV
                     PVAVVVPAEGGITPDDIIAFARERLAGYKVPRRVIIAADIPRVNGWKLLRKRLREEYC
                     GAGGIGCA"
     gene            1627..2214
                     /locus_tag="PLV90_06435"
     CDS             1627..2214
                     /locus_tag="PLV90_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HOF96791.1"
                     /translation="MVKTSSRRSTGIAGLDLVLDGGFPPEAHIIVSGSPLSGLELLAQ
                     QFWRTGNAAGAYLMLDAVPLEGMTDARGMDITALVGAVQGERIVIDSLSTLIADRGID
                     AAIRFIREDVQAAVDEGATIVYLLYAGLHGPFEEARVMRAADVFITLRQQIHGNEIER
                     TLAIEKLRGSNVPQRAIPYHIIARGLELSTTSRVV"
     gene            complement(2211..3113)
                     /locus_tag="PLV90_06440"
     CDS             complement(2211..3113)
                     /locus_tag="PLV90_06440"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOF96792.1"
                     /translation="MTLTSMCLPTTVVGSYPVVKGSGLMSLVDPLKHAVEVAVADQVA
                     AGIDIISDGQVRGDMIHAFTSHLPGIRGSAVVGKVQPARQPITVTDTKYALSRHPKVK
                     GILTGPSTLAHGLKLETSFYRNRDELILDLAQALAVEAGYLQDAGVRLIQIDEPIFST
                     GAANIAVGREAVNAIAGRLRVPVSLHVCGNLGQVIDDVLRIHVAVFDLEFANNPANLD
                     LLSKKDLAGRMIGYGCVDSASPTVESVEIIKKRIEAGIDVLSPSAMLIDPDCGLRMRT
                     RDEASGKLKNMVVAARQVRAEYTD"
     gene            complement(3181..4470)
                     /gene="aspS"
                     /locus_tag="PLV90_06445"
     CDS             complement(3181..4470)
                     /gene="aspS"
                     /locus_tag="PLV90_06445"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050560 - aspartate-tRNA(Asn) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HOF96793.1"
                     /translation="MRLPIRAVTPKTEFAEIIGWVHEVRDLGGLSFFLIRDRTGIIQV
                     TIPKKKVPAEVLDTARNVSRESVVRVRGRVKAIEKAPGGREIVPEELEIISIAESPLP
                     LDVAEKVPAEMDTRIDSRFLDVRKPRVRAIFFIRSTVTCAAMEFLASRGCINITTPKI
                     VAAATEGGTELFPIAYFEKEAFMNQSPQLYKQMMMAAGFEAVFEIGPIFRAEEHNTIR
                     HLNEATSLDVEVSFADHNDVMDLLEDLIVHVYGCVRETCADQLAELGVDLTVPAKPFP
                     RIPYTEAIDIANRAVEEKLSFGDDLSPAAERAIGDVIGQHYFIVDWPTDIRPYYAMPY
                     PDRPEFCKAFDLMHPRMELSSGAQRIHDHDLLVARIRAKGLSPESFEFYLRTFRYGMP
                     PHAGWGLGIERLVMTMLDLSNIREAVLFPRDRHRLMP"
     gene            complement(4614..5156)
                     /locus_tag="PLV90_06450"
     CDS             complement(4614..5156)
                     /locus_tag="PLV90_06450"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HOF96794.1"
                     /translation="MFVPTKCFFTKGVGIHKDKLASFELALRQAGIEKYNLVYVSSIF
                     PPNCQLVSREEGLKHLPTGSIVYVVMARNETNEPSRLASAAIGHAMPKESDAYGYLSE
                     HHAFGETAEVSGEYAEDLAATMLATTLGIEFDPDRAWQEREQIYKASGRIISTTHTCQ
                     AAEGVMDGRWTTVIAAAVFL"
     gene            5712..5954
                     /locus_tag="PLV90_06455"
     CDS             5712..5954
                     /locus_tag="PLV90_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOF96795.1"
                     /translation="MQQDVKPSAVVDCLGLYCPMPIAMTKDAIEKVGIGEVLLVEADD
                     PAAQEDIRRWANRVGHEIVKFEKDGEVIRFSIRRMK"
     gene            5966..6214
                     /locus_tag="PLV90_06460"
     CDS             5966..6214
                     /locus_tag="PLV90_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOF96796.1"
                     /translation="MYADKVVSCIGICQSPMLSIEEEMKMLGEGQILQVMTDKPDLVE
                     MVRSWAGENRHTIEEEHVTSGVTTLILRKGAAAAAEAT"
     gene            6371..6730
                     /locus_tag="PLV90_06465"
     CDS             6371..6730
                     /locus_tag="PLV90_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOF96797.1"
                     /translation="MARILYIQTSGTDMPERLYAPFVLAMTARATDMDADIYFMIRGV
                     TVVKKGAAEAIQVGNFPKLSEMRDQAIAAGATIYICEQSTQLLGIPRGDFIPEGKVVG
                     ALTLNDLAVDADAVMTF"
     gene            6744..7898
                     /locus_tag="PLV90_06470"
     CDS             6744..7898
                     /locus_tag="PLV90_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOF96798.1"
                     /translation="MEGRIYLDHAAAMPVHPGVARFARQFPESFAGNPSTIYREGLFA
                     QEALAEAREKVAALIGAGDPASIIFTSGATESNNIAIRGAALRNKKTGKMVVTSTIEH
                     ISVLNPAKDLQKSGFTFTQVPVDRFGVIDLDALGAAVTPETVVTSVHYANNEIGTVQP
                     IREVAEIVHDRGGFLHADATDAAGRIPINVGRDGIDLLTLSGNQLGGPDGVGALYIRP
                     GVRVQPVLVGGGQERGLRPGTENVFGIACMGEAARLAGEEMPEESPRLVAIRDRLIAG
                     ITGIEEAFLTGHPAERLPHHASFRFSRIEGESIQLAMNALGIAVATGSACTSRTLEAS
                     HVLLAIGLAHDEAHGSMVATLGRRNSLDEVPRVVNAARETVKRLRAITPL"
     gene            7909..8319
                     /gene="nifU"
                     /locus_tag="PLV90_06475"
     CDS             7909..8319
                     /gene="nifU"
                     /locus_tag="PLV90_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HOF96799.1"
                     /translation="MADQIGYSQKVMEHFMNPHNVGVIENPDGYGKVGNPVCGDLMEI
                     FIRVRDDVIEDIRFRTFGCGSAIATSSMVTDLAKGKTLDEALKITRDDVATELDGLPP
                     RKMHCSNLAADALHAAIRDYREKQKTGEGAAQHP"
     gene            complement(8307..9209)
                     /gene="cofD"
                     /locus_tag="PLV90_06480"
     CDS             complement(8307..9209)
                     /gene="cofD"
                     /locus_tag="PLV90_06480"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HOF96800.1"
                     /translation="MITFLSGGTGTPKLLRGMQELLDEREIAVIVNTAEDMWLSGNHL
                     SPDIDTVMYLFADILDTSRWWGIRDDTYITHDLLARLGIEEFIAVGDRDRAVHVARGE
                     MLRRGMRLTEATAALCANLDVFSTVLPMTDSEVTTYVTTRRGDMHFQEYWVKHRGDVE
                     IEGVVRRHKKPPVATGEAIRAIDASDAVVIGPSNPVTSISPILECAGIREALKRKPVV
                     AVSPFIGDAPVSGPAAALMRAFGKESSSAGTYSIYRDLVDIFIQDIRDTATIDGALRL
                     DTLMTNRGKSLDLAKNILTLIYGC"
     gene            complement(9283..>10191)
                     /locus_tag="PLV90_06485"
     CDS             complement(9283..>10191)
                     /locus_tag="PLV90_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HOF96801.1"
                     /translation="MITFLSGGTGTPALIRGARQILYDSEIAVVANTAENLRVSGSHA
                     APDIDTAVFLFAGILDTNRGWGIKGDTYATHNYLPKVAGSEPFAVGDRARAVQIARAA
                     LLREGKTLTEAVQAQCRALNIGATILPATDGDATLLVDTGTECLPLFLYRMSAGTDTE
                     VRELIRAAPEPPAVTGEVRAAIEASDAVVIGPANPASSILPILECAGMRRLLRDTFVV
                     AVSPFRGDFTPDPKDAALMHAVGETPTAPAAFRLYGDIVDVFVQDIHDPDVVEGSLRL
                     ETRLLHERQAESLAWDIMAVIRHAVS"
BASE COUNT         1867 a         3157 c         3349 g         1818 t
ORIGIN      
        1 cggagcccat ccatgccgaa ctgcaccacg tttctcgatg ccaacgccgc taaccccgca
       61 aagaccgctc tcatcatccc ctcgaccgga gagtcctaca cccgtgccga cctcctcgat
      121 acggtcgccc gggccgcggg cggcctcctc gccctcgggg tcgagcgcgg ggagcgcgtc
      181 tgcatctacc tggaaagttc ggtggagtac ctcatcagtt acctcgccct ctggcggatc
      241 ggcgccgtcg cggtcccgac gaaccgggtc taccgcgaga gcgaggtcct ctacgccctc
      301 cgcgacgcgg gggccgcagc cgtcatcacc gacgcggagg gtgcggctct cgtcgggcgt
      361 atacgcgacc gggcgccgac gctccggcac gtcgtcgccg tcagcggcaa catcgccggc
      421 acgacaccgt ggaccgacct cctgcatgca gagccctgcc gtcgcgccgc ccactgccgg
      481 ttcgacgacc tctgccagat ccagtacacg tccggcacca ccgggagacc caaaggcgcc
      541 atgctcaccc acggcaactg gatcgcggcg atggatgccg agcgggatct cctcggaatt
      601 accgcggacg acatctacct cggtatctac ccgatgggcc acgtcgggat cagctggggc
      661 atatccaccc tccgggcagg cgggacctac gtcgtcatgg agcggttcga tctttcccgc
      721 tacatcgacc tcgcccgtga ataccgggcc acgatcgtcg ccggcatgcc gccggtgatc
      781 cactccctcc tccagacccc gcccgggacc gaggcggcgc tcgccaccgt ccgggtgatg
      841 atcagcggcg gcggccccct gatgcccggt gtctggaaac ccttccacga gcggttcggg
      901 atcccggtcg ccaacgccta cggcctctcc gagacggtcg tcgtcgggac cgggaccgcg
      961 atccgtcccg agcactacgc gaccgccgac gagttccgga gcgtcggcac cccggtcggg
     1021 ttctcggagg tgaagatcgt cgatgcagac gatcccgctc aagagctcgg tcccggcgag
     1081 gacggggaga tcgccctccg ggggccgggg gtggcgctcg gctactggca gatgccggag
     1141 gagacccgtg ccgccttcct ccccgacggc tggttcctga ccggcgatat cggccacctc
     1201 gacgcggacg ggatgctcgc catcaccgac agaaagaagg acatgatcgt catgtcgggc
     1261 tggaaggtct acccgaccga ggtcgaggat gcccttatcc agcacccggg catccgtgat
     1321 gcggcggtct tcgggtgccc cgacgagcgc cggggcgagg ttccggtagc cgtggtggtc
     1381 cctgcggagg gcggcatcac ccccgacgat atcatcgcct ttgcccgcga gcgcctcgcc
     1441 ggctacaaag tcccccgccg ggtcatcatc gccgccgata tcccccgggt gaacgggtgg
     1501 aagctcctcc ggaagcgcct cagggaggag tactgcggtg ccggcggcat cggctgcgcc
     1561 tgacggttac tattaagaag gaaaagcggt gtatagatac tcctgacgta cacccgaggt
     1621 gcagatgtgg tcaagaccag cagcagacgt tcgacaggta tcgcgggcct ggaccttgtg
     1681 ctcgatggag gattcccccc ggaagcccat atcatcgtat ccggatcccc tttaagcggc
     1741 cttgagctcc tggcacagca gttctggcgg acggggaacg cggcgggtgc gtacctgatg
     1801 ctcgatgccg tgccgctcga aggcatgacc gacgcacggg gcatggatat tacggcgctg
     1861 gtcggagccg tgcagggaga acggatcgtc atcgactccc tctccacgct gatcgccgac
     1921 cggggcatcg atgcagccat ccggtttatc cgcgaagatg ttcaggcggc cgtcgatgag
     1981 ggagcgacca tcgtttacct cctctacgcc ggcctccacg gccccttcga ggaggcacgg
     2041 gtgatgcgtg cagcggacgt cttcatcacc ctcaggcagc agatccacgg caacgagatc
     2101 gagaggacgc tcgccatcga gaagctcaga ggctcgaacg tgccgcaacg ggcgatcccc
     2161 tatcacatca tcgcccgtgg ccttgaactc tcgacgacga gcagggtggt ctagtccgta
     2221 tactcggccc ggacctgtct cgccgcaacg accatgttct tcagttttcc agacgcctcg
     2281 tcgcgtgtcc gcatccgcag gccgcaatcg ggatcgatga gcatcgcgct cggcgagagt
     2341 acgtcgatcc cggcctcgat cctctttttg atgatctcga cgctctcgac ggtggggctt
     2401 gcagagtcca cgcagccgta accgatcatc cggccggcga gatccttctt cgagagcaga
     2461 tcgaggttgg ccgggttgtt tgcaaactcg agatcgaaga cggcgacgtg tatcctgagg
     2521 acgtcgtcaa tgacctgccc gaggttcccg cagacgtgga gagagaccgg cacccggagc
     2581 ctgccggcga tcgcgttcac cgcctcgcgg ccgacggcga tgtttgccgc ccccgtggag
     2641 aagatgggct cgtcgatctg gatgagacga acgccggcgt cctggaggta gcccgcctcg
     2701 accgcgagag cctgcgcgag gtcgaggatc agctcgtccc tgttccggta gaacgacgtc
     2761 tccagtttca ggccgtgcgc gagcgtgctc gggccggtca ggatcccctt caccttcggg
     2821 tgccgggata gggcgtactt cgtatccgtg acggtgatgg gttgccgggc cggctggacc
     2881 tttccgacga ccgcagagcc ccgtatgccg gggaggtgag aggtgaaggc gtggatcatg
     2941 tcgccgcgga cctggccgtc ggatatgatg tcgatcccgg cggcgacctg atcggcgacc
     3001 gctacctcca ccgcatgctt cagcgggtcc acaaggctca taagcccgga gcctttcacc
     3061 accgggtagc tcccgaccac cgtggtcggg aggcacatgc tcgtcagggt cataagatca
     3121 cctgttccgg tcatggggaa gatgcaccat ttacccttgc gtcacccgtg cagttaatca
     3181 tcacggcatc aaccggtgcc ggtcacgcgg gaagaggacc gcctcgcgga tgttagagag
     3241 gtcgagcatc gtcatcacca ggcgctcgat gccgagcccc catccggcgt gcgggggcat
     3301 accgtagcga aacgtcctca ggtagaactc gaaactctcg ggagagaggc cttttgcacg
     3361 gatccgcgcg acgagaaggt cgtgatcgtg gatacgctgg gctcccgatg agagttccat
     3421 acgggggtgc atcaggtcga acgccttgca gaactccggc cgatcggggt aaggcatcgc
     3481 atagtagggc ctgatgtcgg tcggccagtc gacgatgaag tagtgctgcc cgatcacgtc
     3541 gccgatcgct ctctcggccg cgggagagag gtcgtcgccg aaggagagct tctcttcgac
     3601 ggcccggttc gcgatatcga tcgcttccgt atagggtatc cgcgggaacg gctttgccgg
     3661 gacggtaagg tcgaccccga gctccgcgag ttgatcggcg caggtctccc tgacacaacc
     3721 gtagacgtgg acgatcagat cttcgaggag gtccatgacg tcgttgtgat cggcaaacga
     3781 gacctccacg tcaagcgacg tcgcctcgtt gagatgccgg atggtgttgt gctcctcggc
     3841 gcggaagatg gggccgatct caaagaccgc ctcgaaccct gcagccatca tcatctgctt
     3901 gtagagctgc gggctctggt tcatgaacgc ctctttctcg aagtaagcga tcgggaagag
     3961 ctcggtgccg ccttcggtcg ccgccgcgac gatcttcggg gtggtgatgt tgatgcagcc
     4021 gcgggaggca agaaactcca tcgcagcgca ggtcaccgtc gagcggatga agaatatggc
     4081 gcggacgcga ggtttcctca cgtccaggaa ccgcgagtcg atccgggtat ccatctcggc
     4141 gggcaccttc tccgcgacgt cgagcgggag ggggctttcg gcgatgctga tgatctcgag
     4201 ttcttcgggg acgatctcgc gcccgcccgg ggccttctcg atcgctttga ccctgccccg
     4261 gaccctgacc acggactcgc gcgagacgtt cctcgcggta tcgagaacct ctgccggaac
     4321 cttcttcttc gggatggtca cctggatgat gccggtccgg tcgcggatca ggaagaacga
     4381 gagccctccg aggtcgcgga cctcgtgcac ccagccgatg atctcggcga attcggtctt
     4441 gggggttacg gcccgtattg gtaaacgcat gtgaaaaacc tgggtacaat gtgggcccgg
     4501 ggaggtattc tgtcttatgc tcccgacctg gagttgttcc ggcagggcgg cgggattgtg
     4561 gcctgcaccg gggggagcgg agaaaaaagg gagctgcccc gggaggtgcg gagctacagg
     4621 aagactgcgg cggcgatgac cgtcgtccag cgaccatcca taaccccttc cgccgcctgg
     4681 caggtgtggg tcgtgctgat gatccggccg ctggccttgt agatctgttc gcgctcctgc
     4741 catgcccggt cgggatcgaa ctcgatcccg agcgtcgtcg caagcatggt tgccgcaagg
     4801 tcttcggcgt actcgcccga gacctccgcc gtctctccga aggcatggtg ctcggagagg
     4861 tagccgtagg catcgctctc ctttggcatc gcatggccga tagcagcgga ggcgagcctg
     4921 ctcggctcat tggtctcatt ccgggccata acaacgtaga cgatgctgcc cgtggggagg
     4981 tgctttaacc cttcctccct tgagaccagc tggcagttcg gggggaatat actcgagacg
     5041 tagacgaggt tgtacttctc gatgcccgcc tgacggagag ccagctcgaa cgatgccagt
     5101 ttgtctttgt ggatacccac acccttcgtg aagaaacact tggtcggaac gaacattaag
     5161 gatcttctta tcgtttgaat tttttaagat tttcggttat aacaagcaca aattgaaaac
     5221 ggaaggggat atcctctatt ttttgcatta ggcctttaat atatcgccaa atcataaaaa
     5281 cggctaaaaa ttatcgattt taaaggatcg tcggagtgac cacgacggct cttctgtcta
     5341 ccccctttca cctgcagaat gacgaaaacc agaatcgccg ccctatctcc gtgtatcggg
     5401 gtgcccggaa agatcgggga tgaggggagt tgagcgcatc gggtaagcga tcccggggag
     5461 ggagcgccaa cccgggagac cccgctgcct gcccggggta ccgatctctc aggagtgcgg
     5521 cctctgcatc cgggccctgc gtatggtcct cctgatcctc cggaggggtc accgggactc
     5581 tccggcgctg ctccccacac agcccctcct cccggaggaa cgcttataaa ggatagtgca
     5641 tgatccgctg caaccgggca tgcgccggga gagtgtcgcg gcacccgggt tccacaacgg
     5701 gggatggaga gatgcaacag gatgtcaaac cctctgcggt cgtcgattgt cttgggctct
     5761 actgtcccat gccgatcgca atgacgaaag atgcgatcga gaaggtcggc atcggcgaag
     5821 tgctcctggt cgaggcggac gatccggcag cacaggagga tatccggcgc tgggcaaacc
     5881 gggtggggca cgagatcgtg aagttcgaga aggatggcga agtcatacgg ttctccatcc
     5941 ggaggatgaa gtgaggtggt gttgagtgta cgcggataag gttgtcagtt gcatcggcat
     6001 atgccagtcg cccatgctgt cgatcgaaga ggagatgaag atgctcggcg aggggcagat
     6061 cctgcaggta atgaccgata aaccggacct cgtcgagatg gtcaggtcct gggcaggtga
     6121 gaatcggcac acgatcgagg aggagcacgt cacatcgggc gtgacgaccc ttatcttgcg
     6181 caagggggcg gcggcagcgg cagaggcgac gtgatccgat ggatgccgaa ggttacgcgg
     6241 atgcccgggg tgcctgctgc caggcgccgg ccgtcctgct cggtgagggg gtagatcggc
     6301 cccaaagagg aggagccccg ggaatcgatc tcgatagccg gttttccatc cggaaaggag
     6361 cgtgaatggt atggcacgga tactctacat ccagacgagc ggcaccgaca tgccggaacg
     6421 gttgtatgcg ccgttcgtcc ttgcgatgac ggcacgcgcg acggatatgg atgccgacat
     6481 ctacttcatg atccgggggg tgacggtcgt gaagaaaggc gcggcggagg cgatccaggt
     6541 cgggaacttc ccgaagctct cggagatgcg cgaccaggcg atcgccgcgg gggcaacgat
     6601 ctacatatgc gagcagagca cccagctcct cggcatcccg cggggcgact tcatcccgga
     6661 aggaaaagtc gtcggtgcgc tcaccttgaa cgaccttgcg gtcgacgccg atgcggtcat
     6721 gacgttttaa ggatgacgga gatatggaag gacgaatcta tcttgaccac gcggcggcaa
     6781 tgccggtcca ccccggtgtc gcccggtttg cgcggcagtt cccggagagc tttgccggca
     6841 atccctccac gatctaccgg gaaggacttt ttgcacagga agccctggct gaggcacgag
     6901 agaaggttgc agcgctcatc ggcgcggggg acccggcctc gatcatattc acgagcgggg
     6961 cgaccgaatc gaacaacatc gccatacggg gggcagcgct ccggaacaaa aagaccggga
     7021 agatggtcgt cacgagcacc atcgagcata tatcggtcct gaatccggca aaagatctcc
     7081 agaagagcgg gtttacattc acgcaggtcc cggtcgaccg cttcggcgtc atcgacctcg
     7141 acgccctcgg agcggcggtc acgccggaga ccgtcgtcac gtcggtccac tacgcgaaca
     7201 acgagatcgg gacggttcag ccgatccggg aggtggcgga gatcgttcac gaccggggcg
     7261 ggttcctcca cgccgacgcc accgacgccg cagggcggat accgatcaac gtcgggcgtg
     7321 acggcatcga cctccttacc ctctcgggga atcagcttgg gggcccggat ggcgtcggtg
     7381 cgctctacat ccgcccgggg gtcagggtcc agcctgtcct cgtcgggggc gggcaggaac
     7441 gcggtctccg gcccgggacc gagaacgtct tcggcatcgc ctgcatgggg gaggcggccc
     7501 ggcttgccgg ggaggagatg ccggaggaga gcccgcgtct tgtcgcgata cgggaccggc
     7561 tgatcgccgg catcaccggg atcgaggaag cgttcctcac cggccacccg gcggagcggc
     7621 tcccccacca cgcgagtttc cggttctccc ggatcgaggg cgagagcatc cagcttgcga
     7681 tgaacgccct cggtatcgcg gtcgccaccg ggtcggcgtg cacctcccgg acgctcgagg
     7741 cctcgcacgt cctccttgcc atcgggctcg cccacgacga ggcgcacggg tcgatggtcg
     7801 cgacgctcgg gcgcaggaac agcctcgacg aggtgccccg ggtcgtcaac gccgcaagag
     7861 aaacagttaa acgcctgcgg gctattacac cgctgtaggt gaaggacctt ggcagaccag
     7921 atcggataca gccagaaggt gatggagcac ttcatgaacc cgcacaacgt cggggtgatc
     7981 gagaaccccg acggctacgg caaggtcggc aaccccgtct gcggggacct catggagatc
     8041 ttcatccggg tcagggatga cgtcatcgag gacatcaggt ttcggacgtt cgggtgcggt
     8101 tcagcgatcg cgacgagcag catggtgacc gaccttgcta aaggaaagac gctcgacgag
     8161 gcgctgaaga tcacccgcga cgacgtggcg accgaactcg acggtctgcc gccgagaaag
     8221 atgcactgct caaacctcgc ggcggatgcg ctccacgcgg cgatacggga ctaccgggag
     8281 aagcagaaga cgggtgaagg ggccgctcag cacccgtaga tcagcgtcag tatgttcttc
     8341 gcgagatcca ggctcttccc ccggttggtc atcagggtgt cgaggcggag cgccccgtcg
     8401 atcgtggcgg tatcccggat atcctggatg aatatatcca caagatcccg gtagatgctg
     8461 taggttccgg cggacgagga ttctttcccg aatgcccgca tcagggccgc cgccgggccg
     8521 ctgaccgggg cgtcgccgat gaaggggctg accgcgacga cgggtttcct cttgagcgcc
     8581 tcacggatgc cggcgcactc gagtatcggc gatatgctcg tgaccggatt gctcggcccg
     8641 atgacgacgg catcgctcgc gtcgatcgcc cggatcgcct cgccggtcgc gaccgggggc
     8701 tttttgtgcc ggcggaccac gccttcaatc tctacgtcgc cccggtgctt cacccagtac
     8761 tcctggaagt gcatgtcgcc ccggcgtgtc gtgacataag tcgtcacctc agagtcggtc
     8821 atcgggagga cggtcgagaa gacgtcgagg tttgcgcaga gggccgccgt cgcctcggtg
     8881 agccgcatcc ctctcctgag catctccccg cgggcgacgt ggaccgcccg gtcccggtcc
     8941 ccgacggcga tgaactcctc gatgccaagc cttgcgagga ggtcgtgggt gatgtaagta
     9001 tcgtcgcgga tcccccacca ccggctggtg tcgagtatat ccgcaaagag gtacatgacc
     9061 gtatcgatgt cgggcgagag gtggtttccc gagagccaca tatcttcggc agtgttgacg
     9121 atgaccgcga tctcccgttc gtccagcaac tcctgcatgc cgcggaggag cttcggggtc
     9181 ccggtcccgc cggagaggaa ggttatcatg gagtaattag cgtatcacgc atagtcgtat
     9241 atgtattttc ccaaatcctg ccgaacagca cctcccttca tctcacgaaa ccgcgtgacg
     9301 tatgaccgcc atgatatccc aggcgagcga ctctgcctgc cgttcgtgca ggaggcgggt
     9361 ctccaggcgg agcgagcctt ccacgacgtc cgggtcgtgg atatcctgga cgaagacgtc
     9421 gacgatatca ccatacaacc gaaaagccgc cggtgcggtg ggggtttcgc cgacggcgtg
     9481 catcagggcg gcatcctttg gatccggcgt gaagtcgccc cggaacggcg agaccgcgac
     9541 gacgaacgtg tcgcggagga ggcggcgcat gccggcgcac tcgagtatgg gaaggatgct
     9601 cgatgccgga tttgccggcc cgatgacgac ggcatcgctc gcctcgatcg ccgcccgcac
     9661 ctccccggta accgcgggcg gctcgggtgc ggcgcggatg agctcccgca cctcggtgtc
     9721 ggtgccggcg ctcatccggt agagaaagag tgggaggcat tcggtcccgg tatcgacgag
     9781 gagggtggca tcgccatccg tcgccgggag gatggtcgcc ccgatattga gcgcccggca
     9841 ctgggcctgc actgcttccg tcagggtctt tccctcacgg aggagggccg cccgggcgat
     9901 ctggacggca cgggcacggt cgccgaccgc aaagggttcg ctgccggcca ccttcgggag
     9961 gtagttgtgg gtggcgtagg tgtcgccctt gatcccccac ccgcggtttg tatccaggat
    10021 gccggcaaag aggaagacgg cggtatcgat atccggggcg gcatggctcc ccgagacccg
    10081 gaggttctcg gcggtgtttg cgaccacggc gatctcgctg tcgtagagga tctgccgggc
    10141 gccccggatg agcgcgggcg tcccggttcc gccggagagg aaggtgatca t
//
LOCUS       DAOSII010000070        10154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_6434_length_10154_cov_54.690464, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000070 DAOSII010000000
VERSION     DAOSII010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10154
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6434_length_10154_cov_54.690464"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..961)
                     /locus_tag="PLV90_06490"
     CDS             complement(47..961)
                     /locus_tag="PLV90_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOF96802.1"
                     /translation="MSQDRLPQMVISIMLLADFDISERCNIRPRSFDLIAKKGDTLVI
                     IKVASQIDSVTADIAWDLNLIARYLQATPLIVGERARDAELERGVVYIRYGLFALSSE
                     TLYDYFAEGISPMVYASPGGLYVKIKGDLLREVRERSRMSLGDLASHLGVSRRTISKY
                     ESGMGTTLDVAIRLEELFSAPLVETIELVGYRTPEPENPPKSTVGDVFIDLERMGMEI
                     HAMRQAPFQALALFDRHTILTAYGTTQKVVKRASLIGNISQITKTFAMCVVTDYNKQK
                     KIGKTLLIGEEHLHTLEDGSELIDMINE"
     gene            1008..2261
                     /locus_tag="PLV90_06495"
     CDS             1008..2261
                     /locus_tag="PLV90_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Ile)(2)-agmatinylcytidine synthase"
                     /protein_id="HOF96803.1"
                     /translation="MWIGIDDTDSPAGMCTTYIGAVLVRRLKERGIPITGARLVRLNP
                     NVIHKTRGNAAVCIEAEGDAEAVFALACACVEELAEFDAPETNPGVVVAAVRPPPDFY
                     YAVLRDFCTVDEAVDVLESIGARYRGYKNRRGLIGATAAVASVFFDRTYELLAYRKRE
                     AWGTPRQVDRASLFLADETTYPRTWDTVDRKNDAVVCVPHTPDPVLFGIRGESPAAVR
                     EARSYVRSEEPACEQVYTTNQGTDAHLLPGTIGELREGRSYLVRGAVAGLPATGPGGH
                     VSFLLEDGGVEVRCMAYEPTRGFRDVVRALVPGDRVAVAGSYKGGSLNLEKLGVSHLA
                     ALVRIRPPVCPACARRMTSAGTAKGYKCRVCGGRSREPDVERWERRIRPGWYEVPPVA
                     RRHLSRPLVRGVPAWEEDLLQSGRP"
     gene            2784..3719
                     /locus_tag="PLV90_06500"
     CDS             2784..3719
                     /locus_tag="PLV90_06500"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HOF96804.1"
                     /translation="MTFTYGNAVQQARVRPGMTVGELVDELGRAGAYNGGSLWQAVNI
                     YERMLRDEEALKFFGLSGAMVPGGMGGIVADLIRRRHIDILVSTGANLTHDIIEAIGC
                     HHYHGVADVSDAELCEEGINRIYDVFLPNEAFIRFEEFMQDVYSSLPEGSTVSIAELL
                     NRIGKRLDTGILAEAAKAGVPVYCPAIQDSMIGLQYWLFSQTHKVTVSAFGDMPALLD
                     RCFEAERAGAILVGGGVPKNYILQSKLMTESGFDYAVQLTGDRPDLGGLSGATLDEAR
                     SWGKLTGEATAVTVYGDATISLPLLVAATLERLEG"
     gene            3716..4351
                     /gene="pyrF"
                     /locus_tag="PLV90_06505"
     CDS             3716..4351
                     /gene="pyrF"
                     /locus_tag="PLV90_06505"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOF96805.1"
                     /translation="MTELILSLDVTDRRRAVAVAESCAPFIDAIKVGYPLVLAAGLSI
                     VGDLSGVGLPLIADFKVADIPNTNRLICEAVFSAGFDAVIAHGFVGGDAARACVEVAH
                     NHGGAAYIVAEMSHPGATEYFHGGVAEHLAALAVACRADGIIAPATRPDRIGRLREIV
                     GERTIYSPGVGAQGGDLDTVARLVDGVIVGRSIYEAADPGAEAERFSRIRR"
     gene            4467..4829
                     /gene="nrdD"
                     /locus_tag="PLV90_06510"
     CDS             4467..4829
                     /gene="nrdD"
                     /locus_tag="PLV90_06510"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOF96806.1"
                     /translation="MNWSPEQQKLVEKYRSLNDIPAGERRYKCHTCHFVVDETPCPHC
                     GETALEIMCPLDHCDCHHSIVESIEYCPLCGHAVCPECGSHDVVQISRVTGYLQDVAG
                     WNAGKQQELKDRVRYSAV"
     gene            4826..5713
                     /locus_tag="PLV90_06515"
     CDS             4826..5713
                     /locus_tag="PLV90_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HOF96807.1"
                     /translation="MRHFVRDDTLFLRGRFRAASTGINGGIADVTTVLNHTVSRDFCD
                     EPQRYLELLAARHGLFHEYFGLLTAVEMRHLCVLQYDFITVFITAGVTNPTPPVDNAP
                     HTINIVVHSREGVCDAALLETIVTATGAKARALHDLGYDFPGTTTDAVVVACERDVPG
                     AHTYAGTLTEAGRRVHAAILYGVPEALARHQGKVRRSGPSFFIYSRYGGDHWVEWVKE
                     GCPYYPCHFPGQRCDYCYCPCYPCADEELGEWVESSNGGRVWGCAGCTLLHVPAIADY
                     VKRNPEATLAELKRLRERL"
     gene            complement(5722..5916)
                     /locus_tag="PLV90_06520"
     CDS             complement(5722..5916)
                     /locus_tag="PLV90_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOF96808.1"
                     /translation="MALTADSTIAELLREKPESVQVLFRFGMGCLGCAIANNETIREA
                     AQVHGIPLEEMLSALGVAEA"
     gene            6191..7417
                     /locus_tag="PLV90_06525"
     CDS             6191..7417
                     /locus_tag="PLV90_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HOF96809.1"
                     /translation="MNLSEVTERISRKLEAKGAQPDRQKIESRLRRLVEEFGVNAAEA
                     ERTVTGDLAREYKVTNIGGTSTELRPISDIVPGEWVTIEGKIVALTPALSPSIAQSGI
                     VADSSGAMRFVTWTRANAPAMEYGHWYRIESAVVDEYKGAPNLKIHSGTTISRMEKDA
                     PLLPSITPIAELKPGVGSVRAKVVQDWEVSHERMLQTGLLGDETGTIKFVTWNEEGKD
                     KLDLDAVYNIFYATVDEYNGRLSLTLNTAMYITDEGDIEVGRSETEIQGALVHIAPGS
                     GLIKRCPVEGCNRTLSRQNYCPVHEIQPNFRYDLRLKAVLDDGIRARNVLVQRELVEK
                     LAGITLDEAVGIAESNPIGMDEIFYRIRNAVLGRYYTCSGSEFGGRLLASSCTPVAFK
                     PEELAALLNRAGGESA"
     gene            7414..7980
                     /locus_tag="PLV90_06530"
     CDS             7414..7980
                     /locus_tag="PLV90_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleic acid-binding protein"
                     /protein_id="HOF96810.1"
                     /translation="MTPQTQFEREPARRVFASELRETRYQFKDGDDEKSPTYVILPTG
                     IRSNRVFIVGTLTEKLRQGDQNIFYRGRIVDPTGTFFVMAGSYQPEAMQQLARIEPPA
                     FVAVVGKPSLYTTPDGTCLVSVRAESISIVDRETRDLWVLDTARETLDRLDAFGTTDD
                     SRKAQEEYGTQPAIYRKIVYDALSQVQL"
     gene            complement(8080..8427)
                     /locus_tag="PLV90_06535"
     CDS             complement(8080..8427)
                     /locus_tag="PLV90_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOF96811.1"
                     /translation="MDERGPGEGRGHRGRGRPRVRRMIGDGGAFRCFGPLCGRPDEVV
                     LLLPEEIEALRLVDLAGFEQEEAAIALGISRKTLWRDLHEARKKVADALVHGKTIQIV
                     GCGRRRGERCPGG"
     assembly_gap    8756..8855
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8856..9768)
                     /locus_tag="PLV90_06540"
     CDS             complement(<8856..9768)
                     /locus_tag="PLV90_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96812.1"
                     /translation="MRHTLWILVLLFALIGAASAIPALPAEFSGTVTIDENPAPAGTA
                     ITARIGDRDCGSLTLKTAGTFGGDSTFDKRLLVNGEDTDLGKAIAFFVDGISAGTATY
                     TPGTSTDLALAVTKGDTPDSESSDGTPPGSSGSGSPSGGSFTPPAAPATEYTGSGTLD
                     TDARGAVQHAVVIAAAGGDARLTIAEGVLAQDRFGRPLDTVSVETAPPGDATRAVRCG
                     PDGATFDPAIEVSFALTPEEWEQAGQFVVRWYNSTADVWEPLATTIHASTRTVTATIS
                     HFTLVAVFAVPADTPEDTVTATPAGSET"
     gene            complement(9774..>10154)
                     /locus_tag="PLV90_06545"
     CDS             complement(9774..>10154)
                     /locus_tag="PLV90_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96813.1"
                     /translation="AGDWTALDEQDRLAPLEGFWIYSAGPTAVPLNFSTNPLLPPAER
                     TLVAGWNAIGTTGNTPATARDTLYSVKGRWTTLIGFDARNQAFETGIVNGGSGANTDT
                     RSVYPGRGYWLSMTGPGTLYAIGA"
BASE COUNT         1803 a         3148 c         3298 g         1805 t
ORIGIN      
        1 ttgttaagcg atagaaaggt aggtttgtag ttctatataa tagtattcac tcgtttatca
       61 tatcgatgag ttctgagcca tcttcgagag tgtggagatg ctcttcgccg ataaggagcg
      121 ttttacctat cttcttctgc ttgttgtaat ccgtgacgac gcacatggca aatgtcttcg
      181 tgatctggga tatattgccg atgagggaag cacgctttac aaccttctgg gtggtgccgt
      241 aggccgtcag gatggtgtgc cggtcaaaga gcgccagagc ctggaacggg gcctgccgca
      301 tcgcgtggat ctccatcccc atgcgttcga gatcgatgaa gacgtcgcca actgtggact
      361 tcgggggatt ttcgggctcg ggggtgcggt agccgacgag ttcgatcgtc tcgacgagcg
      421 gggcgctgaa gagttcctcc agccgtatcg cgacatcgag cgtggtgccc atgccgctct
      481 cgtacttgct gatggtccgg cgggagaccc cgaggtgcga cgcgagatcc ccaagcgaca
      541 tccgggagcg ctcgcggact tcgcggagga gatcgccctt gatcttcacg tagaggcctc
      601 ccggggatgc atagaccatc ggcgatatcc cttctgcaaa gtagtcgtag agcgtctcgg
      661 agctcagggc aaagaggccg taacggatat agacgacgcc gcgctcgagt tccgcgtcgc
      721 gagcgcgctc cccgacgatg agcggggtgg cctggaggta ccgggcgatc aggttgaggt
      781 cccaggcgat atcggcggtc acgctgtcga tctgggaggc gactttgata atgacgaggg
      841 tgtcgccttt cttcgcgata aggtcgaaac tccgcggacg gatgttgcac cgctccgaga
      901 tatcgaagtc cgcgaggagc atgatgctga tgaccatctg aggaaggcga tcctgcgaca
      961 taacccgtaa gaatcgatat agtcggagaa gatataaatt atagcgtatg tggatcggga
     1021 tcgacgatac ggattctcct gccgggatgt gcaccaccta catcggggcg gtgctggtgc
     1081 ggcggttgaa agagcgtggg atccccatca ccggggcccg gctggtccga ttgaacccga
     1141 acgtcatcca caagacccgg gggaacgcag cggtctgcat cgaggccgaa ggtgatgcag
     1201 aggcggtgtt tgcgcttgcc tgcgcgtgcg tggaagaact cgcggagttt gatgccccgg
     1261 agacgaaccc gggcgtggtg gtcgccgccg tccggccgcc gccggatttt tactacgcgg
     1321 tgctccggga tttctgcacc gtggacgagg cggtcgacgt gctcgaatcg atcggagccc
     1381 gttatcgcgg ttataagaac cggcgcggcc ttatcggggc gaccgcggca gtcgcgagcg
     1441 tcttcttcga ccggacgtac gagctgctcg cctaccgaaa gcgggaagcg tggggtaccc
     1501 cgcggcaggt ggaccgcgcg agcctctttc tggccgatga gacgacctac ccccgcacct
     1561 gggatacggt ggaccgaaag aacgatgccg tcgtctgcgt gccccacacg cccgatccgg
     1621 tcctcttcgg catccgcgga gagagcccgg cagccgtccg cgaagcgcgg tcgtacgtcc
     1681 gctcggagga gcctgcctgc gagcaggtct acaccaccaa ccaggggacg gacgctcacc
     1741 tgctgccggg gacgatcgga gaactccggg aggggcggtc ctacctggtg cggggcgcgg
     1801 tcgccggact gccggccacc ggacccggcg gccacgtctc gttcctcctc gaggacggcg
     1861 gcgtcgaggt tcgctgtatg gcctacgagc cgaccagggg gtttcgcgac gtcgtacgag
     1921 ccctcgtccc cggggataga gtggctgtcg cggggagcta caaggggggg agcctcaacc
     1981 tcgagaagct cggggtctcc caccttgccg ctctggttcg catccgcccg ccggtctgcc
     2041 cggcctgcgc gagaaggatg acgagcgccg ggactgcgaa gggctataag tgccgggtct
     2101 gcggcgggcg cagccgcgaa ccggatgtgg agaggtggga gcgccggatc cggccggggt
     2161 ggtacgaggt cccccccgtc gcccgccggc atctctcccg gccgctggtg cgcggtgtcc
     2221 cggcatggga ggaagatctc cttcaatccg gccggccatg acctccacgg gcggccgtgc
     2281 cccggggaga cgccatgggc ggagttgcct cgtcaatcaa actggcttta caaaataaag
     2341 tttataatgt ttgtgagtta acttatccct ggtgatcccg tgccgaccat cagcggtgat
     2401 ggtgcacgtg tcctgacctg tctgcccatg acggtgcgac ccacggcaga tggggttcag
     2461 cggcaggctg cacagaaccg cggtcgagta cgcgtggagc agagacgaag gcatccctcc
     2521 ggagatcctc cggtctcccg gcccgaaagt ccgctctctc acgagactat ccggctcata
     2581 tccggatctc cgacacggcg ggacgagcgg gatcatcccg tgtcggccaa ccagtcatcc
     2641 cctgtctggc agcggggacc cgatctatgg gttcctgcaa catctccata catttttggt
     2701 cgtcggacgt tgccacaggg aggagacgag gtgttattgt gcatgaatgc caataccatc
     2761 tcacgaactg gtgtaacgga atcatgacct tcacgtatgg gaatgcagta caacaggcgc
     2821 gtgtgcgccc cggtatgacg gtcggcgagc tcgtcgacga actcggaaga gcaggggcct
     2881 acaacggagg atccctgtgg caggcggtca acatatacga gcggatgctc cgcgacgagg
     2941 aggcgctgaa gttcttcggc ctctcgggcg ccatggttcc cggcgggatg gggggcatcg
     3001 tcgccgacct catccggcgc cggcacatcg atatcctggt atcgacgggt gcgaacctca
     3061 cccacgacat catcgaggcg atcggctgcc accactacca cggggtcgcc gacgtctccg
     3121 acgccgaact ctgcgaggaa gggatcaacc ggatctatga cgtcttcctc ccgaacgagg
     3181 cgttcatcag gttcgaggag ttcatgcagg atgtctactc ctccctcccg gagggttcga
     3241 cggtctcgat tgccgaactc ctcaaccgga tcgggaagag gctcgatacc gggatcctcg
     3301 ccgaagcggc aaaggccggg gtgccggtct actgcccggc aatccaggac tcgatgatcg
     3361 ggctgcagta ctggcttttt tcccagacgc ataaggtcac ggtcagcgcg tttggagata
     3421 tgcccgccct cctcgaccgg tgttttgagg ccgaacgggc cggggcaatc cttgtcgggg
     3481 gcggcgtccc gaagaactac atccttcaga gcaaactgat gaccgagagc gggtttgact
     3541 acgcggtcca gctcaccggc gaccggcccg acctcggcgg actctcgggc gcgacgctcg
     3601 acgaagcgcg gtcgtggggc aaactcaccg gagaggccac cgccgtgacg gtctacgggg
     3661 atgcgaccat cagcctgccg ctccttgtgg ccgcaaccct cgagaggctg gaaggatgac
     3721 cgaactcata ctctctcttg acgtcaccga ccggaggcgg gcggtggcgg tcgcagagtc
     3781 ctgcgcaccc ttcatcgatg cgatcaaggt cggctacccg ctcgtcctcg cggccggcct
     3841 ctctatcgtc ggggatctct ctggtgtcgg cctcccgctc atcgcggact tcaaggtcgc
     3901 ggatataccg aacaccaacc gcctcatctg cgaggcggtc ttctcggccg gatttgatgc
     3961 cgtcatcgcc cacgggttcg tgggaggtga cgcggcgcgg gcctgcgtcg aggtggcgca
     4021 caaccacggt ggagccgcct acatcgtcgc ggagatgagc catcccggtg cgaccgagta
     4081 cttccacggc ggcgtggccg aacacctcgc cgcgctcgcg gtcgcctgcc gggccgacgg
     4141 tatcatcgcc cccgccaccc ggcccgaccg gatcggtcgg ctccgcgaga tcgtcggcga
     4201 aagaacgatc tactccccgg gcgtgggggc gcagggcggc gacctcgata cggtggcccg
     4261 gctggtcgac ggcgtcatcg tcgggaggag catatacgag gcggcggacc cgggcgctga
     4321 ggccgaacgg ttctcccgca tccgtcgatg atgaattctc cgttccgatc cctccgggat
     4381 atgacgaacc ctatgagtgg tctgaggcgg atgcggaccg ttccgcatct gaagggttaa
     4441 tatattttcc tgcagatact cgtatcatga actggagccc agaacagcag aaactggtgg
     4501 agaagtaccg gagcctcaac gatatacccg ccggcgagcg gcgctacaag tgccacacct
     4561 gccacttcgt cgtggacgag accccctgcc cgcactgcgg cgagactgcg cttgagatca
     4621 tgtgcccgct cgatcactgt gactgccacc actccatcgt cgagagcatc gagtactgcc
     4681 cgctctgcgg ccatgcggtc tgccctgagt gcgggagcca cgatgtcgtc cagatcagca
     4741 gggttaccgg atatctccag gacgttgcag gctggaatgc gggcaaacag caggagctga
     4801 aagaccgggt ccgctactct gccgtatgag gcatttcgtc agggacgata cgctcttcct
     4861 ccgcggccgg ttccgggcgg cgagcaccgg catcaacggt ggtatcgctg acgtcacgac
     4921 agtcctgaac catacggtgt cccgcgattt ttgcgacgaa ccgcagcgct acctcgaact
     4981 tctcgccgcc cggcacggtc tcttccacga gtatttcggg ctcctgaccg ccgtcgagat
     5041 gcggcacctc tgcgtgctcc agtacgactt cattacggtc ttcatcaccg ccggggtgac
     5101 gaacccgacg cctcccgtcg acaacgcccc gcacaccatc aacatcgtcg tccacagccg
     5161 cgagggggtc tgcgacgccg cgctccttga gacgatcgtc acggcgaccg gggcgaaagc
     5221 ccgggcgctc cacgatcttg gctatgattt ccccggcaca acgacggatg cggtcgtcgt
     5281 cgcctgtgaa cgtgacgtcc ccggcgcgca tacctatgcc gggacgctga ccgaagccgg
     5341 gaggcgggtc cacgcggcca tcctctacgg cgtgccggaa gccctcgcgc gccaccaggg
     5401 aaaggtccgg cggagcggac cgtcgttctt catctacagc cggtacggcg gcgatcactg
     5461 ggtggagtgg gtgaaggagg gatgccccta ctacccctgc cacttccccg gccagcggtg
     5521 cgactactgc tactgcccct gctacccctg tgccgacgag gagctcgggg agtgggtcga
     5581 gagttcaaac ggcggcaggg tctgggggtg tgccggctgt acactcctgc acgtccctgc
     5641 gatcgcggat tacgtaaaaa ggaatcccga ggccacgctc gccgagctca agcgcctccg
     5701 ggaaaggttg tgagagggga ttcacgcctc ggcaacgccg agggcggaga gcatctcttc
     5761 gagggggatg ccgtggacct gggcggcttc ccggatggtc tcgttatttg cgatggcgca
     5821 gccaaggcaa cccatgccga accggaaaag cacctgtacc gactcgggtt tctcccgaag
     5881 gagttctgct attgtactgt ccgcagttaa tgccatacat ctgatgttat ccctgccggt
     5941 atatatacgt tcaagagcca gggcggatat cccctgatct ggttgcagac ttcggatcgg
     6001 gatattctgc cgctcccatc gggcacgggc aatcttttcg ggcggcatat ccgcggccga
     6061 cccatcactg ttgcagtcct accccggggt cgcgcccggc accatggcga ggagccatga
     6121 gtttcacttt caccccgcac gccgagcctt aataccctcg cgaggtgaag tatagactgg
     6181 agatgtatgc atgaacctat ccgaggtaac agaaagaatc tcccggaaac ttgaagcaaa
     6241 aggtgcacag cccgatcgac agaagatcga gtcacgcctt cggcgcctcg tcgaggagtt
     6301 cggtgttaac gccgccgagg ccgaacggac ggtgacgggc gatctcgccc gcgagtacaa
     6361 ggtcaccaac atcgggggga cctccacgga acttcgcccg atcagcgata tcgttcccgg
     6421 cgaatgggtg accatcgagg gaaagatcgt tgccctgacc ccggcgctct ccccctcgat
     6481 cgcgcagagc gggattgttg ccgactcaag cggcgccatg cgcttcgtga cctggacccg
     6541 ggcaaacgcc cctgcaatgg agtacggtca ctggtaccgg atcgaatccg cggtcgtcga
     6601 tgagtacaaa ggcgcaccga acctgaagat ccactcgggc accaccatct cccggatgga
     6661 gaaggatgcc ccgcttctcc cgtcgatcac gccgatagcg gagctgaaac cgggcgtggg
     6721 aagcgtgcgg gccaaggtcg ttcaggactg ggaggtctcc cacgagcgga tgctccagac
     6781 ggggctcctc ggcgacgaga ccggcaccat caagttcgtc acctggaacg aggaggggaa
     6841 ggataaactg gatctcgacg ccgtctacaa tatcttctac gccacggtag atgagtacaa
     6901 cggcaggctc tccctcaccc tgaacaccgc gatgtacatc acggatgagg gcgatatcga
     6961 ggtcgggcgg agcgagaccg agatccaggg cgcccttgtc cacatcgccc cgggctcggg
     7021 cctgatcaag cgctgccccg tcgaagggtg caaccggacc ctctcccggc agaactactg
     7081 cccggtgcat gagatccagc ccaacttccg ctacgatctc cgcctgaagg cggtcttaga
     7141 cgacggcata cgtgcgagaa acgtcctggt gcagcgcgag ctcgtcgaga aactcgccgg
     7201 gatcaccctt gacgaggcag tcgggatcgc ggagtcaaac ccgatcggca tggacgagat
     7261 cttctatcgc atcaggaatg cggtgctcgg gcgttactac acctgtagcg ggagtgagtt
     7321 tggcggccga ttgcttgcca gttcctgcac gcccgttgcg ttcaagcccg aagaactggc
     7381 tgcactcctg aaccgcgccg gaggtgagtc ggcgtgacgc ctcagacaca gttcgagcgt
     7441 gagccggccc gcagggtctt tgcatccgag ctccgggaga cccgctacca gttcaaggac
     7501 ggcgatgacg agaagagtcc gacctacgtc atcctcccga ccggtatccg gagcaaccgc
     7561 gtcttcatcg tcggaaccct cacggagaag ctgcggcagg gcgaccagaa catcttctac
     7621 cgcgggcgga tagtcgatcc cacaggcacc ttctttgtca tggcgggatc ctaccagccc
     7681 gaggcgatgc agcagctcgc ccggatcgaa ccgccggcct tcgtcgcggt cgtcgggaaa
     7741 ccaagcctct atacgacccc ggacggcacc tgcctggtct cggttcgtgc ggagtcgata
     7801 tcgatcgtcg accgtgagac ccgcgatctc tgggtgctgg ataccgcccg cgagacgctg
     7861 gacaggctgg atgcgttcgg gaccaccgac gactcacgga aggcgcagga ggagtacggg
     7921 acgcagccgg cgatctaccg taagatcgtc tacgacgccc tctcgcaggt gcagttgtag
     7981 ggctgcacac cgctcctgac attttttaga gctaaaatag agcggatccc gcgccacctc
     8041 aatggcggga tgagatcatg ggctgcgggt tcgcctcatt catcccccgg ggcacctctc
     8101 tccccgccgg cggccgcacc cgacgatctg gattgttttc ccgtgcacaa gggcatcggc
     8161 aaccttcttc cgcgcctcgt ggaggtcgcg ccagagggtc tttcgagata tgccgagtgc
     8221 gatcgcggcc tcttcctgtt cgaaccccgc aaggtcgacg agccgcaggg cctcgatctc
     8281 ctcggggagg aggaggacaa cctcgtcggg ccgcccacag agggggccga aacagcgaaa
     8341 cgctcctcca tcgccgatca tccggcgaac ccgggggcgg ccccgcccgc ggtgaccgcg
     8401 gccctcaccg ggtcctctct cgtccatctt caacactcct ggagaagaga tccgcgccgg
     8461 atgaagatag tatcttcgct ccggcatcgg tcgtcaccac cggcgtcgcg cccggccgca
     8521 tctccatggg acaccgcccc ggcccgggtg tgaggggctc tctcccccgc ccggaaggcg
     8581 atcaaccccg cttccggatg aggagggccg ccccggccag ggccgcgagc ccggcgagcc
     8641 ccgggagaga ttgaccggcc tgctgcacag tggttgccgg ggctgcaggc gtggaggtcg
     8701 gggtctccga gccggcgggg gttgccgtca cggtgtcctc cggggtatcg gccggnnnnn
     8761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngggtc tccgagccgg cgggggttgc
     8881 cgtcacggtg tcctccgggg tatcggccgg cacggcaaag accgcgacca gggtgaagtg
     8941 cgatatcgtt gcggtgactg tgcgggtcga ggcgtggatc gtcgtcgcaa ggggttccca
     9001 gacgtcggcc gtgctgttat accaccggac gacgaactgc ccggcttgtt cccactcctc
     9061 cggtgtgagg gcaaacgaga cctcgatcgc ggggtcgaag gtcgcgccgt cgggaccgca
     9121 ccggactgcc cgggtggcgt cgcccggtgg ggcggtctcg acgctcaccg tatcgagcgg
     9181 tctcccgaag cggtcctgtg ccagaactcc ctctgcgatg gtaaggcggg cgtctccacc
     9241 ggcggcggcg atgaccactg cgtgttggac cgcgccccgg gcatcggtgt cgagagtgcc
     9301 gctcccggtg tactctgtcg ccggtgctgc aggaggtgtg aatgaaccgc cgctcgggct
     9361 tccactaccc gatgacccgg ggggggtgcc gtctgaggac tcgctgtcag gcgtatctcc
     9421 tttcgtcacc gcgagagcaa ggtcggtcga ggtgccgggg gtgtaggtcg ccgtcccggc
     9481 ggagatcccg tcgacgaaga atgcgatcgc cttcccgaga tcggtgtcct ctccgttgac
     9541 gagcaggcgc ttatcaaacg tggaatctcc gccaaaggtt ccggctgtct tgagcgtgag
     9601 cgacccgcag tcgcggtcgc cgatccgggc ggtgatggcg gtcccggccg gcgccgggtt
     9661 ttcgtcgatc gtgaccgtgc ccgagaactc cgcggggagg gcggggatgg cggacgctgc
     9721 cccgatgagg gcgaagagaa gtacaaggat ccagagtgtg tgtctcatca gtatcatgct
     9781 cctatggcat agagggtgcc cggcccggtc atggagagcc agtagccccg gccggggtag
     9841 acggatcgcg tgtcggtgtt tgcgccgctc ccgccgttga cgatcccggt ctcgaaggcc
     9901 tggttcctgg catcaaaccc gatcagggtc gtccaacgac ctttgaccga gtagagggtg
     9961 tctcgcgccg tcgccggggt gttgccggtg gtcccgatag cgttccagcc ggcaacgagg
    10021 gtgcgctccg cgggcgggag gagggggttg gtcgagaagt tcagcgggac tgccgtgggg
    10081 ccggccgagt agatccagaa cccctcgagg ggggcgaggc gatcctgctc atcgagcgcc
    10141 gtccagtccc ctgc
//
LOCUS       DAOSII010000071        10116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_6461_length_10116_cov_42.145115, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000071 DAOSII010000000
VERSION     DAOSII010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10116
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6461_length_10116_cov_42.145115"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            680..1882
                     /locus_tag="PLV90_06550"
     CDS             680..1882
                     /locus_tag="PLV90_06550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOF96814.1"
                     /translation="MKVALLCEYSRDMGAYQGSNIYITELITRLAGRDDIDLHLITMG
                     RENAVRQEEGFTHHILRKGGPLSLPYFHPLVLVRMVKTVRAIRPDIVHALSTRYPCST
                     AAVLLQNDYPSLVTAFGIFEREIAHYREDMPPLERTLSHIFHTFFIRNERWVLSRAPE
                     LVVDAPSIGDLVRTPTTGRIHVVAAGLDLERLRPQILSPPAGEGPDIVFINTLTRLKG
                     ADILLSALPGVVEAYPGIRACIGGKGPQAPELRRLARDLGLEANVIFPGFVPDEEKYR
                     YYRQCRMVVVPSRWDCQPSPLFEAAAFGKPVVASDMAHPGIVEDGVTGLIFRSDDAAG
                     LEEKILFLLDDDAARERMGRAAAARVGEYDWPRVVDRYVTIYQDAVREHRRGGKRATA
                     PAGASPER"
     gene            1895..3016
                     /locus_tag="PLV90_06555"
     CDS             1895..3016
                     /locus_tag="PLV90_06555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOF96815.1"
                     /translation="MRESTIGLLTFPIERSGIHPTSNLIGILSSLTREPIPLVTGNAG
                     RAFENDARVRFSGVDHAGGGSVASRVLQNIATQVRMARRIREVDGTGTWLFFFGGEVL
                     VLPMLAAKLSGKKVILALPGHTPLMMAPARDRLLLPAGILTGITCRLCDRIVLYSPAL
                     VDHWGLGQYREKITIAREHLLDFEAFRVKVPLDERPMAVGYIGRLADEKGVWNFVRAL
                     PRLAADTDLHFLIGGDGPLLPAIREFIRESGLSERVTLAGWIRHDDLPCHLNRLRLLV
                     MPSCTEGLPNIMLEAMACGTPLLAAPVGAVPDFIHDGVTGFILAPNTPEGIAASVIDI
                     LGRSELTSIAGAARKCVEQEITYERAVERFAAVIEGLGD"
     gene            3022..4893
                     /locus_tag="PLV90_06560"
     CDS             3022..4893
                     /locus_tag="PLV90_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96816.1"
                     /translation="MHLDKVLAGTGIVVSAALVAYIALTTGRLIFILAGVLTLICCLL
                     WLAVRERAIREFPVSWAGGAETGNDRSGTVLLILFFALYVLSIAALHTRASLYERPTG
                     YFVLSSLMAGVIALEILLPHRRKARRVLPQIVLLGVSVVWSQILIVPGILGSDPWWHR
                     MFVSQILDLSAIPGGYWYTHMPLFHLEVAGASLLAGLDYRLAAMLSVSLAQIIILPVV
                     VYLLGNALFEDERVGLLAGLLLITANQQIGMSTASIPNAFAAIFIALVLFLLFRLKYT
                     RPVRAGYLAYLLLAAIILTHTIAAACMALILFVVWGACRVHGRIRDDAAAKPYPVTLG
                     LASLFLAAMLGWWYFVSGHIAVLADLIRQGFTRAYFIQNPAVVDAYPGLVPVPEQVFN
                     NIGMFAFFTVSLVGIFYLAARRERNGFAFGMVGIILLGIGFFPLITGLSFIEHRWWYF
                     SQILLAVPLAASLLLVAGREKAHRMPAALTLAACVGLLAFTMIASPAADTGIPLFSPN
                     SAVRAGFTGSELQAIDTVGGIRAGAIGADRYVQVVNYVNPGPGFVVPADDMLIEGAYR
                     SHGMDAVLVREYIRGHPIFCYDGMFRLTHDPAMTLAREGYYRVYESGSVGLFRLDGL"
     gene            4962..5465
                     /gene="fae"
                     /locus_tag="PLV90_06565"
     CDS             4962..5465
                     /gene="fae"
                     /locus_tag="PLV90_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HOF96817.1"
                     /translation="MVSFEHLLIGEALVGEGPEVAHIDLVIGKKGSSVEQAFVTALAS
                     PARGHTPLLAVLTPNIPAKPATLMVNKVTIRNAGQALLIFGPAQAAVAKAVVDSVAEG
                     IIPGAEADNLLIIASVFIEWDAADKKKIYDWNYEATKLAIRRAVAGEPKMQEVLARKD
                     TAKHPFA"
     gene            complement(5478..6221)
                     /locus_tag="PLV90_06570"
     CDS             complement(5478..6221)
                     /locus_tag="PLV90_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96818.1"
                     /translation="MIPLFPLPAAAVLAAVHLVAGRLRFLDVVPRSRWLSLAGGVSVA
                     YVFVRLLPELSAGQEVVQRAIAGELLAGLENHIYIVALLGLAIFYGLERVVRRSQEER
                     STTQDIERTTTGPGVFWLHIASFGVYNILIGYLLLHRIRPGLMSLAFYTVAMALHFLV
                     TDYGLRNDHRRDYDRVGRWALSIALAGGLAAGYASRLPELWIAVITAFLAGGVILNVL
                     KEELPEERKSRIAPFFIGAFGYAAILLLA"
     gene            complement(6254..7651)
                     /locus_tag="PLV90_06575"
     CDS             complement(6254..7651)
                     /locus_tag="PLV90_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOF96819.1"
                     /translation="MGLQRGLSQFDLTNIIVGAIVGADIYIASALTAGLVGPFSIVLW
                     AVAGLFAATIAMVFAYCSYYVPRVGGPFAYVSEAFDDFYGFLTGWSMWIAELLALPVF
                     AIAFTNYLQALVPLTTAAEIAVRALFVASLTLVNIVGVRAAGRLNDLLTLIKLAPLLL
                     LIVAGLGIFLIRPEVFIGNYTPLLPFGLDNASHALVLVFWAYAGFELGTLPAGEVENP
                     QTAIPRAITTGMLIVAVFYLLTNFVLFGLVNWTLLDRSAVPLVVAGTALFGSAGAIIM
                     TVGALFSVSGSNESGMLGSARLAYAMAIDGLFPRAFARLHPRFNTPHVVLIAQGIIAF
                     ILSNIGNLSNLISFAVLTLAFSFLLTCFALIVLRDDNAAHLRGQHILPLAGIGVCLFL
                     LFSTTMLDKVAGVLVILAGIPIYLFYSPKQDIRHLKALFLSEEAVLRRRLEAKETYLA
                     NLLDIIRTVSRKIGR"
     gene            7775..7945
                     /locus_tag="PLV90_06580"
     CDS             7775..7945
                     /locus_tag="PLV90_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOF96820.1"
                     /translation="MRFRIVVREDPEDGGYVVSCPAIPGCHSQGETLEEALENIKDAI
                     SGCVEVLNERTE"
     gene            complement(8027..8098)
                     /locus_tag="PLV90_06585"
     tRNA            complement(8027..8098)
                     /locus_tag="PLV90_06585"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8063..8065),aa:Gly,seq:gcc)
     gene            complement(8252..8947)
                     /locus_tag="PLV90_06590"
     CDS             complement(8252..8947)
                     /locus_tag="PLV90_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HOF96821.1"
                     /translation="MVAIEPLVLLVLALGPGVFWLWYFYHRDKYEPEPAALIVKVYLL
                     GVLVTFPVAFVEGFVGLLIASPLIMGVIIAPIVEEYGKFTVVYRFVYRDAEFDEPMDG
                     IVYAAAAALGLASLENVLYVLTAYVTSPTLAFETLAIRAIFSVPAHALFSSVWGYALG
                     RAKFTAAERRPGIIFRGLALAMMLHGTFNLLLFLSDIVAYATVIFVLVLIPGLWILAN
                     RNIRIALRWQRRR"
     gene            complement(9008..9739)
                     /locus_tag="PLV90_06595"
     CDS             complement(9008..9739)
                     /locus_tag="PLV90_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96822.1"
                     /translation="MVVLGIIAVVLAIALVWMLIGATMQFVFVDMLSTGDIHIRRFFR
                     ERLGKGTRLFLFELGITVAMVLAIMALFLILFGFGSIGTGAAAALLIIALIPIILVAA
                     LVFGLIFLLTTDFVVPIMIREDCGVIEGWQRLIGVISAGFWQTAAYVVVRFVVGLIAA
                     IVQAVLVIIALLIIAIPFVLIGVVLLAAFQGGNIALFVVLLIPYLIIAIPAALLIAVP
                     FVTFFRYYALLVLEGLGPGYRLLPE"
     gene            9797..10111
                     /locus_tag="PLV90_06600"
     CDS             9797..10111
                     /locus_tag="PLV90_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96823.1"
                     /translation="MILEDVWYTDAASYKESDHGEAEPDAPLYRPEQAFHAMKSAFQP
                     VECIDLFSHMSQEEKDLLAFKTSSGQAGAGLSVRGHMPGSLRGGRFFNTLIYSVHHTI
                     EE"
BASE COUNT         1922 a         3201 c         3235 g         1758 t
ORIGIN      
        1 cttctccagg atctccccat gctgtggacc gtggggatat cgccattggg ggaggggctg
       61 acggggaggg ggaacttgtt ccccctcccc tgtctcccac actccaacca tcttacttct
      121 cgccggcatc ccttgcccgc gccttcgcag ggatacccat acccttctcg ccggcacccc
      181 caccccccgc gccttcgcca ctccgccccc gcccccgccc cggggcaggg gcagtgcgtg
      241 gcgatatcag gggagcagcc gtatccgggg tgcagccggt gggaaagccg tgcccggaga
      301 tcggcgttgt gcgccggccc tcgtggcgag gcccggtccc cgttcttctc cggcctatcc
      361 ccacggggtg gaccgtggcg tctctcgcca cggggggggc cggccatgcc cggcatctat
      421 ccggacggac gcccgccctt ctcccccttc tccggcctgt ttccgtatat ttcaggtgag
      481 gtgacggctc ccggcgccgg ctgcccgccc ggtctctctg gcggcaacgt ttcggcagcc
      541 tgcatgtggg ccggtgcggt ttgcggtgca gggctcctca aactatatat gggttgatgg
      601 tgattgcatc cctgcgccac atgactggac cgttgaagaa ccctatcccg gcggagcagc
      661 acagtccggg gaaaatatca tgaaagtagc ccttctctgc gagtactcac gggacatggg
      721 ggcataccag ggttcaaaca tctacataac ggagttgata acacgcctcg ccgggcgtga
      781 cgatatcgat ctccatctca tcaccatggg cagggagaac gccgtacggc aggaagaggg
      841 gttcacccac catatcctcc ggaagggcgg cccgctctcc ctcccctact tccaccccct
      901 ggtgctcgta cggatggtga agaccgtcag ggccatccgg ccggatatcg tccacgccct
      961 ctccacccgg tacccctgct ccacggccgc cgtcctcctc cagaacgact acccctccct
     1021 cgtgaccgcg ttcggcatat tcgagagaga gattgcgcac taccgcgagg atatgccccc
     1081 cctcgaacgg acgctctccc atatcttcca caccttcttc atccggaacg agcggtgggt
     1141 gctctcgcgg gcgcccgagc tcgtcgtgga tgccccctcg atcggggatc tcgtcaggac
     1201 gccgacgacc ggccggatcc acgtcgtcgc cgccggcctc gaccttgaac ggctccggcc
     1261 gcagatcctc tcccccccgg ccggagaggg gccggacatc gtcttcatca acacgctcac
     1321 ccggctgaaa ggcgcggata tcctgctatc cgcccttccg ggcgttgtag aggcgtatcc
     1381 cggcatcagg gcgtgcatcg ggggaaaggg tcctcaagca cccgaactcc ggagactcgc
     1441 ccgggatctc gggctggagg cgaacgtcat cttccccggt ttcgtgcccg acgaggagaa
     1501 gtaccgctac taccggcagt gccggatggt ggtcgtcccc tcccggtggg actgccagcc
     1561 gtcccccctc ttcgaggccg cggcgttcgg caaacccgtc gtcgcatcgg acatggctca
     1621 tcccggcatc gtcgaggacg gggtgaccgg gctcatattc cgctccgacg acgcggccgg
     1681 gcttgaagag aagatcctct ttctcctcga cgacgatgcc gcacgcgagc gcatgggccg
     1741 tgccgccgcg gcacgggtcg gcgagtacga ctggccgagg gtcgtcgacc ggtacgtcac
     1801 gatataccag gacgccgtca gggagcaccg gcggggcggg aagcgggcta cggcgcccgc
     1861 cggtgcctcc ccggagaggt gaggagagac tgccatgaga gagagtacca tcggactgct
     1921 cacgttcccg atcgagcgat cgggcatcca cccgacatct aacctcatcg ggatcctctc
     1981 gtcccttacg cgtgaaccga tccctctggt gacggggaac gccgggcgcg ccttcgagaa
     2041 cgacgcgcgg gtccggttct ccggcgtcga tcacgcgggc gggggaagcg tggcatcaag
     2101 agtcctgcag aacatagcaa cgcaggtgag gatggcccgc cgcatcaggg aggttgacgg
     2161 caccggcacc tggctcttct tcttcggcgg cgaggtcctc gtcctgccga tgctcgcggc
     2221 aaaactctcc ggaaaaaagg tcatactggc gctcccgggc catacccccc tcatgatggc
     2281 gccggcacgt gaccggctcc tcctcccggc ggggatactc accggtatca cctgcaggct
     2341 ctgcgatagg atcgtcctct actccccggc cctcgtagac cactggggac tcggacaata
     2401 ccgggagaag ataacgatcg cccgcgaaca cctcctcgac ttcgaagcgt tcagggtaaa
     2461 ggttccgctc gacgagcggc ccatggccgt cggctacatc ggccgcctcg ccgacgagaa
     2521 aggggtctgg aacttcgtcc gcgccctccc gcgccttgct gccgataccg acctgcactt
     2581 tctcatcggc ggcgacggac ccctgctccc ggctatccgg gagtttatcc gggagagcgg
     2641 cctctccgaa cgggtgaccc tcgcgggatg gatacgccac gacgacctcc cctgccacct
     2701 caaccgcctc aggctgctcg tgatgccgtc ctgcacggaa gggctcccga acatcatgct
     2761 cgaggccatg gcctgcggca cgcccctgct cgcggctccc gtgggcgccg tgcccgactt
     2821 catccatgac ggcgtgacgg ggtttatcct ggcgccaaac acgccggagg gcatcgcagc
     2881 ctcggtcatc gatatactcg ggcggtcaga gctgacatcc atcgcaggag ccgccagaaa
     2941 gtgcgtagag caggagatca cctacgaacg ggcagtggag cggtttgccg ccgtcatcga
     3001 agggctcggg gattgacggc aatgcacctc gataaagtcc tcgccggtac cggcatcgtc
     3061 gtctcggccg ccctcgtcgc ctacatcgcg ctcaccaccg ggcgcctgat cttcatcctc
     3121 gccggcgttc tcaccctcat ctgctgtctc ctctggcttg cagtccgcga gcgggcgatc
     3181 cgggagttcc cggtatcgtg ggccggcggg gcagagaccg ggaacgaccg gtccggcacc
     3241 gtgctgctca tcctcttctt cgccctctac gtgctgagca tcgccgcgct ccatacccgc
     3301 gcctcgctgt atgagcggcc aaccggctac tttgtcctct catcgctcat ggccggcgtc
     3361 atagccctcg agatcctcct gccgcaccgg agaaaggccc gccgggtcct gccgcagatc
     3421 gtcctcctcg gcgtatcggt cgtatggtcg cagatcctta tcgtcccggg catactcggc
     3481 tcggatccct ggtggcacag gatgttcgta tcgcagatcc tggacctctc cgccataccg
     3541 ggaggttact ggtacaccca catgccgctc ttccacctgg aggttgccgg ggcctcgctc
     3601 cttgccgggc tggactaccg cctcgcagcg atgctctccg tgagcctggc acagatcatc
     3661 atcctcccgg tggtcgtcta cctcctcggg aatgccctct ttgaggacga acgggtcggg
     3721 ctgctcgccg ggctcctgct catcaccgca aaccagcaga tcggcatgag cactgcatcg
     3781 atcccgaacg ccttcgccgc gatattcata gcccttgtcc tctttctcct cttcaggctg
     3841 aagtatacac ggccggtgcg ggccgggtat cttgcatacc tcctccttgc cgccatcatc
     3901 ctgacgcaca ccatagcggc ggcgtgcatg gcgctgatcc tcttcgtggt gtggggcgcg
     3961 tgccgggtcc acggccgcat ccgggacgat gcggccgcaa aaccgtatcc cgtcacgctg
     4021 ggcctcgcgt cgctcttcct ggccgccatg ctcgggtggt ggtacttcgt ctcagggcac
     4081 atagcggtcc ttgccgacct catccgccag gggttcaccc gggcctactt catccagaac
     4141 ccggcggtcg tcgacgcgta tcccgggctc gtgccggtcc cggaacaggt cttcaacaac
     4201 atcggcatgt tcgccttctt caccgtaagc ctcgtcggca tcttctacct tgccgcacgg
     4261 cgggagagga acgggtttgc cttcggtatg gtggggatca tcctccttgg catcggcttc
     4321 ttcccgctca tcacgggcct ctcgttcatc gaacaccggt ggtggtactt ctcccagatc
     4381 ctccttgccg tcccgctcgc ggcgtcgctt ctcctggttg cagggagaga gaaggcccac
     4441 aggatgcccg ccgcactcac ccttgccgcg tgcgtcggcc tccttgcctt caccatgatc
     4501 gcaagcccgg cggccgatac cggcatcccg ctcttctcac cgaacagcgc cgtacgggcc
     4561 gggtttacgg ggtcggaact ccaggcaatc gatacggtgg gcggcatacg cgccggggct
     4621 atcggggcgg acaggtacgt ccaggtggta aattatgtca acccgggccc ggggttcgtc
     4681 gtgccggccg acgatatgct gatcgagggg gcgtatcgtt cgcacgggat ggatgccgtc
     4741 ctggtccggg agtacatccg gggtcacccc atcttctgct acgacggcat gttcaggctg
     4801 acgcacgatc cggctatgac gcttgcccgg gagggctact accgggtgta cgaatccggc
     4861 tcggtcggcc tgttcaggct tgacggcctg tagtgccggc gccctctcca caaccttttt
     4921 cttccccgca ccccatccca caacccggga ggtttactac catggttagt tttgaacatc
     4981 tactcatcgg agaagcgctc gtcggcgagg ggccggaggt tgcccacatc gatctcgtca
     5041 tcgggaagaa ggggagcagc gtggagcagg ccttcgtcac ggcgctcgca tcgcctgccc
     5101 ggggccacac gccgctgctt gcggttctca ccccgaacat cccggcaaag cctgcgacgc
     5161 tgatggtcaa caaggtcacg atccggaacg ccggccaggc cctcctgatc ttcggcccgg
     5221 cacaggctgc cgtcgcaaag gcggtggtgg atagcgttgc ggagggtatc attcccgggg
     5281 cggaggccga taacctgctg atcatcgcat cggtcttcat cgagtgggat gcggccgaca
     5341 aaaagaagat ctacgactgg aactacgagg cgacgaagct tgccatccgc cgggcggtag
     5401 cgggcgaacc gaagatgcag gaggtgcttg cacggaagga taccgcgaag catccttttg
     5461 cgtgattgcc gtcgctttca cgccagaagg aggatggcgg catacccgaa cgcgccgatg
     5521 aagaaaggcg ctatacggct cttgcgctcc tcggggagct cttccttgag cacgttgagg
     5581 atgacgccgc cggcgaggaa cgcggtgatg acggcgatcc agagctccgg gagccggctt
     5641 gcgtagcctg ccgcaagacc gccggcgagc gctatggaga gcgcccatcg ccctacccgg
     5701 tcgtagtccc ggcgatgatc gttacgcagc ccgtagtcgg tgaccaggaa gtggagcgcc
     5761 atggcgacgg tatagaatgc aaggctcatg aggccgggcc gtatacggtg gagcaggagg
     5821 tagcctataa ggatgttgta gacgccgaag gatgcgatat ggagccagaa gactcccggg
     5881 ccggtcgtcg tgcgttcgat atcctgcgtt gtcgaccgtt cctcctgcga cctcctgacg
     5941 acccgctcca gaccgtagaa gatggcaaga ccgaggagcg cgacgatgta gatgtggttc
     6001 tcaaggccgg cgagtagctc cccggcgatg gctctttgga caacctcctg gccggcgctc
     6061 aactcgggga gcaggcggac gaagacgtag gcaacggaga cacctcctgc gagagagagc
     6121 cagcggctcc gcggtacgac gtcgaggaac cgcagcctgc ctgcgaccag gtgcaccgcc
     6181 gccaggacgg cggctgcagg gagcgggaag agagggatca ccggctcccc ctcgaggcgg
     6241 caggggataa cggttacctc ccgatctttc gggataccgt acggatgata tcgagcaggt
     6301 ttgccagata cgtctccttt gcctcgagtc ttcgccggag cacggcctct tcggagagga
     6361 agagggcctt gagatggcgg atgtcctgtt ttggggagta aaagaggtag atcgggatgc
     6421 cggcgaggat gaccaggacc cctgcgacct tatcgagcat cgtcgtcgag aagaggagaa
     6481 agaggcagac gccgatgccg gcgaggggaa ggatgtgctg gccgcggagg tgtgcggcgt
     6541 tgtcgtctcg cagcacgatg agggcgaagc aggtgagcag gaacgagaac gcgagggtga
     6601 ggacggcaaa cgatatcaga ttggagagat tcccgatatt ggagaggatg aacgcgatga
     6661 tgccctgggc gatgaggacc acgtggggcg tgttgaagcg tgggtggagc cgtgcgaagg
     6721 cgcgggggaa gagcccgtcg atagccatcg catacgcgag gcgggcgctg ccgagcatcc
     6781 ccgactcgtt tgaccccgag accgagaaga gggcgccgac cgtcatgatg atcgcccccg
     6841 ccgacccgaa gagggcggtc ccggcgacga cgagcgggac ggcgctccgg tcgaggaggg
     6901 tccagttgac gagcccgaag aggacgaagt tcgtgaggag gtagaagacg gcgacgatca
     6961 gcatcccggt ggtgatggcg cgggggatgg ccgtctgcgg gttctctacc tcgccggccg
     7021 ggagcgtccc gagctcgaac ccggcgtatg cccagaagac caggaccagg gcgtgggagg
     7081 cgttatcgag cccgaaaggg agcagggggg tgtagttccc gatgaagact tccggccgga
     7141 tgaggaagat gccgagaccg gccacgatga ggaggagcag gggtgcgagt ttgatgagcg
     7201 tgaggagatc gttcaacctc cctgcggccc ggacgccgac gatgttgacg agggtcaggg
     7261 atgcgacgaa gagcgcccgg acggctatct ctgcggctgt tgtgaggggg acgagcgcct
     7321 ggaggtagtt cgtgaacgct atcgcaaaga ccgggagggc gaggagttcg gcgatccaca
     7381 tgctccagcc ggtgagaaac ccatagaagt cgtcgaacgc ctccgaaacg taggcgaacg
     7441 gccctccgac gcgaggaacg tagtaactgc agtaggcaaa gaccatcgcg atcgtcgcgg
     7501 caaagagccc ggccaccgcc cagaggacga tcgagaacgg gccgacgagg cccgccgtga
     7561 gggcggacgc gatgtagatg tcggcgccga cgatcgcccc gacgatgatg ttcgtcaggt
     7621 cgaactgcga gagcccccgc tgtaatccca tgccgcggga agaggagggg gcagcggcga
     7681 taaagattcc gcaccggtat gcgccggccc cgtgcgcaac cgccgggcaa accctcatca
     7741 catccggggt cgtagaatgg tcgggtgcat aagggtgaga ttccgcattg tcgtcaggga
     7801 ggatccggag gatggcgggt atgtggtgag ttgccctgca attcccggat gccactcgca
     7861 gggcgagacg cttgaagagg ctcttgagaa catcaaagat gccatatccg ggtgcgttga
     7921 ggtattgaac gagcgaacgg agtgagcatc cgggcggctc gccgcgagag ggtggtcact
     7981 ctgcgaaaga ttctaaaaaa cagcgcaccg gtgcggtgct ttcgagtgcg ccgaccggga
     8041 ctcgaacccg ggtttaggcg ttggcaacgc ctagtgataa ccactacact atcggcgcac
     8101 accagtgctc tgcactgtgt gccttacaaa gtgggatctc ctccataata agtctatcga
     8161 atcgcagtcc ggggcgggcg gcatcgcccc tgcggtatcc gcacgatgag gcgctctcag
     8221 ggcggtattc gccacccctc cgcgccggca cctaccgccg cctctgccac cgaagcgcga
     8281 tccggatgtt ccggtttgca agtatccata gccccggtat caggaccagg acgaatatga
     8341 ccgttgcgta ggcgacgata tcggagagga agagcaggag attgaaggtg ccgtgcagca
     8401 tcatcgcgag agcaagcccc cggaatatga tacccggccg tcgctcggca gccgtgaact
     8461 tggcccggcc gagggcgtag ccccagacgc tggaaaagag cgcgtgagcg gggaccgaga
     8521 agatcgcccg gatggcgagc gtctcgaagg caagcgtcgg cgacgtcacg tatgccgtga
     8581 ggacgtagag gacgttctcg agggatgcga gcccgagggc ggccgcggcg gcgtagacga
     8641 tgccgtccat cggctcatcg aactcggcgt cccggtagac gaaccggtat acgaccgtga
     8701 acttgccgta ctcctcgacg atgggcgcta tgatgacgcc catgatcagc ggggatgcga
     8761 tgaggaggcc gacgaacccc tcgacgaagg ccacggggaa ggtgaccagg acaccgagga
     8821 ggtatacctt cacgatcagg gctgccggct cgggctcgta cttatccctg tggtagaagt
     8881 accataacca gaataccccg ggccccagcg ccaggacaag gagcaccagc ggttcgatcg
     8941 caaccattac ccggccgtag tcatgttctc atcagggaag agcctttctc atggccgaaa
     9001 aaaagggtta ctccgggagg agccggtacc ccggtccaag cccttccagc accagcaggg
     9061 cataataccg gaagaacgtg acgaacggca ccgcgatcag gagcgcggcc gggatggcga
     9121 tgatgaggta ggggataagg agaacgacga acagagcgat gttccccccc tggaatgcgg
     9181 cgaggagcac taccccgatg agcacgaacg gtatcgcgat gatcaggagc gctatgatga
     9241 ccaggaccgc ctgcacgatt gcggcgatga ggcccaccac aaacctcaca acgacgtagg
     9301 cggcggtctg ccagaacccg gccgatatca cgccgatgag ccgctgccag ccctcgatga
     9361 cgccgcagtc ctcgcggatc atgatcggga cgacaaagtc ggtggtcagg aggaatatga
     9421 gaccgaatac gagagctgcg accaggatga tcggtatgag cgcgatgatc aggagcgctg
     9481 cggcggcgcc ggtgccgata ctcccgaacc caaagagtat caagaagagc gccataatgg
     9541 cgaggaccat ggccacggtg atgccgagct cgaaaaggaa gagtcttgtt cctttgccga
     9601 gccgttcgcg gaagaaccgc ctgatatgga tatccccggt cgagagcata tcgacgaaga
     9661 cgaactgcat cgtagcaccg atcagcatcc agacaagagc gatcgcgagc acgacggcaa
     9721 tgatcccgag cacaaccatc ggtaaaatct cggggaagga gccggcatta ccggctaaaa
     9781 aatcaccctc ctcgaattga tactggaaga cgtttggtat actgatgccg cctcctacaa
     9841 agagagcgat cacggcgagg cggagccaga tgccccactt tatcggccag agcaggcttt
     9901 tcatgcgatg aaaagcgcct tccagcctgt tgaatgcata gatctcttca gccatatgtc
     9961 tcaagaagaa aaggatctcc tcgcctttaa aacctcctcc gggcaagccg gcgccggtct
    10021 atcggtccgg ggacatatgc cggggagcct gaggggaggc cggtttttca acaccttaat
    10081 ctattccgtt caccacacta tagaagaatg atccct
//
LOCUS       DAOSII010000072        10015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_6536_length_10015_cov_46.474498, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000072 DAOSII010000000
VERSION     DAOSII010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 10015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10015
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6536_length_10015_cov_46.474498"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1151
                     /gene="dinB"
                     /locus_tag="PLV90_06605"
     CDS             90..1151
                     /gene="dinB"
                     /locus_tag="PLV90_06605"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOF96824.1"
                     /translation="MHVDMDSFFASIEVRRDPSLAGRPVIVGADPKGGAGRGVVSTCS
                     YEARRYGVHSGMPISRAYDLCPHGVYLPVDRNLYVRTSADIMAILSRHAGRIEQVSID
                     EAYLDVSDAGGFPAAEALAAAIKQEVRETVGLTCSVGVAPGKAVAKIASDYKKPDGLT
                     VVRPEEVAGFLAPLPVEKIPGVGRKTGEDLRQMGIRTIGDLAGHDIQALIARLGRSGI
                     SMHRLARGIDEAEVQGREGSKSIARETTFDADTADPDMLSGVVADLADDVAGTLAAAG
                     LRCRTVTVKVRYRGFETHTRSRTLPRFTADPEAIHRAASALLLPFLNGRPVRLIGVRL
                     STLEGGRTRQTAIDEFVGT"
     gene            1205..2038
                     /locus_tag="PLV90_06610"
     CDS             1205..2038
                     /locus_tag="PLV90_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96825.1"
                     /translation="MSDIWIVLLIVAIVLLFTVPVAQQQMVRTRRLRAIRDLETERRT
                     RVIALIHRQERIGFLGIPLFRYIDINDSEEVLRAIRLTPPDMPIDLVVHTPGGLILSS
                     EQIAMALRRHRGKVTIFVPHYAMSGGTLLCLAADEVAMDENAVLGPVDPRIGEYPAAS
                     ILRVPRLKPPEEIEDRTFILVDIAAKAQNQMREFVTVLLRDRMDAERADRLSRLLTQG
                     TWTHDYPITFEEARDLGLPVTPGMPAGVYRLMDLFPQAMPRRPSVAYVPVPYQGEEKK
                     G"
     gene            2124..2555
                     /locus_tag="PLV90_06615"
     CDS             2124..2555
                     /locus_tag="PLV90_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HOF96826.1"
                     /translation="MSTSIQLNLDAFDRTLPPLCGDDPDRNGFRRLCCRCPFAVRSEG
                     SIHCERFAIPIRAREKYALRGWKEVRDTILERDGHRCAVCGGEGDLHIHHVDCDPTND
                     DPGNLVTLCGICHARVHAGLHRAGGAERVARVMKAARRRPG"
     gene            2642..3490
                     /locus_tag="PLV90_06620"
     CDS             2642..3490
                     /locus_tag="PLV90_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOF96827.1"
                     /translation="MGGTSGMGIAERLFGRGDPGDREDPDADAAVSRKAISLAQRGQF
                     DEAIACFDRVLALDPANAKMLNNKGVFLDLLGRDQEALACWEKALSIDPEFAPAWVSR
                     GMLYRRRNRFDEALACYDRAVELNPNSAVAWYNRSGVFAAMRRLDEAVACYERVLALD
                     PHFVAAWTDLGYARFLQHRHEEAIACYDRAIADDPEGVRVWSLKGGALYALGEYRKAL
                     ECFDRVLAIDPKYAAGWSMKCSVLYHLGMYRHALACADKALAINPSCELTTQVRKMLL
                     SLIEKW"
     gene            3621..4475
                     /locus_tag="PLV90_06625"
     CDS             3621..4475
                     /locus_tag="PLV90_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOF96828.1"
                     /translation="MPVDQIPIGTFSRITHLSQKALRHYDERGLLVPAARDVCTGYRY
                     YTFAQAERGLRIRHLLWLGFDLSEVEAVLAARESGDAGAIRDLFRARLAATEREALRL
                     HAIAEVLRTQDPLNGGFHMSITEPAIKDVPAQRALSLRERGTYHEAIPRLIGELLTYV
                     CPEDGRRPAARMAGPVIFICHDEEYRETDADIEVAIPIIGPVSLENSRVDIVTLPAGR
                     FVSVLHTGPYPGVGKAYERLFACMGERGLIPAGPSRELYLNNPGEVSEDELLTEIQFP
                     VQEIPPSP"
     gene            complement(4486..5946)
                     /locus_tag="PLV90_06630"
     CDS             complement(4486..5946)
                     /locus_tag="PLV90_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOF96829.1"
                     /translation="MNTPRYPSLYEVNARVRVRQIGRRATLDDIPDTWLAGLAACGFS
                     WVYLMGAWTTGDAGLRVSRSHEEWLSGYRSLIPDIGEDDICGSPFAVAAYRLHPDLGE
                     PETLERFRERLHRHDLLLMLDLVPNHTAPDHPWVRDHPDYYVHGTEEDLARRPQDYAG
                     VDPGIVLAHGRDTFSGGWPDTLQLDYSNPDLREAMIAEVQGIARKCDGLRCDMAMLVL
                     PEVFRQTWGRDMEPFWPKAIGAVRENLPEFVFMAEVYRDLEWTLQQQGFDYTYDKRLY
                     DRLRWGDARSVREHLRADPAYLDKSVHFLENHDEPRAAAVFSPDRHRAAAVLAFLSPG
                     LRFFHQGQFAGRTRQTPVHICREPEDPGDPAIEEFYRRLSASLRLPAFRDGDWRLLDC
                     NPAWEGNPSHGGYIAFAREREGERCLVTVNYAPDRGQCYVPLPWADIAGKGIRLRDLM
                     GEAAYDRDGESLLSPGLYLDIPGWGFHLFEIIPKGG"
     gene            complement(5987..7198)
                     /locus_tag="PLV90_06635"
     CDS             complement(5987..7198)
                     /locus_tag="PLV90_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HOF96830.1"
                     /translation="MEDQPAYDVVVIGGGPAGSTAACLLARFGHRVALLEKETYPREK
                     VCGGCLSQKSIRFLERVFDLPVPALHEAGLLDSTGTGYALYIRDSRVLAGDLSEPLYF
                     TRRERYDAFLARMAADAGADIYEGAEVVAVDHARRTVTTSSGDRYAARVLIGADGINS
                     RVRRSLPESVVDRQRWQENLGWALELTIPRTEVKTLADGDGAIHLDADLATPQIVLAA
                     CRWGYGWVFPNREALIVGVGGLLTKNKGNQRGRFTEFLKTLGLSAFTKRRPAGYFLPF
                     GNFISSPAHEGTLLVGDAGGFASPILGEGIFYAHRTAELAAHAVDRHLTSGAPAGETY
                     TALLDRRLTPELQAEAALRGYLYGGLEGRTHALLKTFMRASSTRVIDAVQGSRSFRGF
                     RRDGDLHSAVW"
     gene            7556..8638
                     /locus_tag="PLV90_06640"
     CDS             7556..8638
                     /locus_tag="PLV90_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOF96831.1"
                     /translation="MSVAEQKLSHLTAASRFDLCCSGECIRTLFGASHITYNPRAEGC
                     ERMLKVLLHGACSYDCAYCDVRLQRERLSLSPDELAGTFLGFWREGLVDGLFLSSGIP
                     RDVDLVMHELVETGEILRRCGYDGYLHLKVLPGAARADIHDAARVANRISINLEATSP
                     DRLGGIAGVKDYRQDILKRQAWVAEEKPGHHTTQLVLGAAGETDAEVLSCVADQYRRV
                     RPSRIYFAAFRALPGTPLAVHPDTPAWRARRWYQADYLLRDYGVTPDTLRVALDEEGF
                     FANRDPKEVIAAGNAPVNVNTAPRRALLMVPGIGPKGADRILVQRRVRPFTGPADLAR
                     AGIRGKAAGRHLTFGGGETVQTRLVL"
     gene            complement(8652..9707)
                     /locus_tag="PLV90_06645"
     CDS             complement(8652..9707)
                     /locus_tag="PLV90_06645"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOF96832.1"
                     /translation="MAEATLEQQVRRGEGQEYLASEDIVGKRVKNPEGYDLGEISSLR
                     VGLPTGRVAYAVLRTGGMFGLGAKHFAIPLEAIVYRPGDDVFVVNISKYRLDNEPGFT
                     EGEWPKEANWNLIEARRPVGPPSQEEARAVTRTEPPFREVVTTERVEVREKASREEKP
                     IKAEEVEKTGTAAETRTEETRPTVETKTAGATVVEERSETRMKAPAHLSAADLQVYLK
                     GMDYPAGRQDLIDRARKNNAPESVIATFEGFGDRTYRSPAEVSEEFGSKTHGERRETA
                     VERPPETRTRAPVHLSAADLQSYLRGMDYPAGRQDLVARAKENGAPESVIDVIEQFGD
                     RTYRSAAEVSEEFGKIR"
BASE COUNT         1606 a         3425 c         3248 g         1736 t
ORIGIN      
        1 ccccaccaaa ccccctatat cgccggacct ccaacacttt caagagagat ccagggggag
       61 ttcatcgagt ggccgccgat cggatcgtca tgcacgtcga tatggacagt ttctttgcct
      121 ccatcgaggt ccgccgcgac ccctcccttg ccggccggcc ggtgatcgtc ggcgccgacc
      181 cgaagggcgg ggcggggcgt ggggtcgtga gcacctgctc ctacgaggct cgccgctatg
      241 gggtgcactc cggcatgccc atatcacggg cgtatgacct ctgcccgcac ggcgtctacc
      301 tcccggtgga ccgcaacctc tacgtgcgga catcggcgga tatcatggcg atcctctcgc
      361 ggcatgccgg ccgcatcgaa caggtgagca tcgacgaggc ctacctcgac gtcagcgatg
      421 ccgggggttt cccggcggcg gaggcgcttg ccgccgcgat caagcaggag gtccgggaga
      481 cggtggggct cacctgctcc gtcggcgtcg ccccgggcaa ggccgtggcc aagatcgcgt
      541 ccgactacaa aaaacccgac ggcctgacgg tcgtccgccc cgaggaggtc gcggggttcc
      601 tcgcccccct gccggtcgag aagatccccg gcgtcgggag aaagaccggt gaagacctgc
      661 ggcagatggg catccggacc ataggagacc tcgcgggcca cgatatccag gcgctcatcg
      721 cccggctcgg gcgatccggt atctcgatgc accgcctcgc ccggggcatc gacgaagctg
      781 aggtgcaggg cagggagggg agcaagtcca tcgcccggga gacaacgttc gatgcggaca
      841 ccgccgaccc ggatatgctc tcgggcgttg ttgccgacct cgcggacgac gtcgcgggca
      901 cgctcgccgc cgccggcctc cggtgccgga ccgtcacggt caaagtccgt taccgggggt
      961 ttgagacgca tacccggtcc cggacgctcc cgcggttcac cgcggacccg gaagcaatcc
     1021 atcgggcggc atccgctctc ctcctgccgt tcctcaacgg ccggcccgtc cggctcatcg
     1081 gtgtcaggct ctcgacgctt gaaggcggcc gcacccggca gacggcgatc gacgagttcg
     1141 tcggcacctg acggatcacg cagggcgaac cttctttacc cggagcgccc accataccgc
     1201 cggtatgtcc gatatctgga tcgtgctcct catcgtcgcg atcgtcctcc tctttacggt
     1261 gccggtcgcc cagcagcaga tggtccggac ccgccgcctg cgggcgatcc gggacctcga
     1321 gacggagcgc cggacccggg tcatcgccct catccaccgg caggagcgga tcgggtttct
     1381 gggcatcccg ctcttccgct acatcgatat caacgactcc gaagaggtcc tccgggccat
     1441 ccggctcacc ccgccggata tgccgatcga tcttgtcgtc cacacccccg gcggcctgat
     1501 actctcgtcc gaacagatag cgatggccct ccggcgtcac agggggaagg tgacgatatt
     1561 tgtccctcat tatgccatgt cgggcgggac gctcctctgc cttgccgccg acgaggtggc
     1621 tatggacgag aacgccgtgc tcggcccggt cgacccccgg atcggcgagt acccggcggc
     1681 gtcgatcctc cgggtccccc ggttaaagcc gccggaggag atcgaggata ggacgttcat
     1741 cctggtcgac atcgcggcaa aagcccagaa ccagatgcgg gagttcgtca cggtcctcct
     1801 ccgtgaccgg atggacgccg aacgggcgga ccgcctctcg cgcctgctca cgcagggaac
     1861 gtggacccac gactacccga tcaccttcga agaggcgcgg gacctcggtc tccccgtcac
     1921 ccccggcatg cccgccggtg tctaccggct gatggacctc ttcccgcagg cgatgccgcg
     1981 ccgcccgtcg gtcgcctacg tcccggtgcc ttaccaggga gaggagaaga agggctgata
     2041 cccccgcgat atcgtcgcca ttctgacccg aaagacgggg gttcccagca cttatgccgt
     2101 cctgcaacca actattcctg accgtgagca cctccatcca gctgaacctg gacgcgttcg
     2161 accgcacgct cccccctctc tgcggagacg accccgaccg gaacggtttt cgccggctct
     2221 gctgccggtg cccgtttgcc gtacggagcg agggcagcat ccactgcgag cggttcgcga
     2281 tccccatccg ggcgcgcgag aagtacgccc tgcggggctg gaaagaggta cgcgacacca
     2341 tcctcgagcg cgacgggcac cggtgtgccg tctgcggcgg ggaaggggac ctccacattc
     2401 accacgtcga ctgcgacccc acgaacgacg acccgggaaa cctggtcacg ctctgcggca
     2461 tctgccatgc ccgggtgcac gccggcctcc accgcgcggg cggggcggag agggtggcgc
     2521 gggtgatgaa ggcggcacgc cgccgtcccg gctgaccctc ccgatacacc ctcagggtgc
     2581 cccgccggta tcaacggcag gggaacggtt atattgtatg cccgctacca gatgcgtgac
     2641 catgggggga acgtcgggaa tgggtatagc agaacgactc ttcgggcggg gcgatcccgg
     2701 ggaccgggag gacccggatg ctgatgccgc agtgagcagg aaggctatct ctctcgccca
     2761 gcgggggcag tttgacgagg caatcgcgtg ttttgaccgg gtgctcgcgc tcgatccggc
     2821 caacgcaaag atgttgaaca acaagggcgt cttcctcgac cttctcggca gggaccagga
     2881 agcgctcgcc tgctgggaga aggcgctctc gatcgatccg gagttcgctc cggcctgggt
     2941 ctcccggggg atgctgtatc ggcgccgcaa ccggttcgat gaggcgctcg cctgctacga
     3001 ccgcgccgtg gaactcaacc cgaactccgc ggtcgcctgg tacaaccgga gcggcgtctt
     3061 tgccgcgatg cgccggcttg acgaggcggt cgcctgctac gagcgggtcc tcgcgctcga
     3121 cccccatttc gtggcggcct ggaccgatct cggttacgcc cgattcctgc agcaccggca
     3181 cgaggaggcc atcgcctgct acgatcgggc catcgccgac gaccccgagg gcgtcagggt
     3241 ctggagcctg aagggcggcg ccctgtacgc cctcggggag taccgcaaag cgctcgaatg
     3301 cttcgaccgg gtgctcgcga tcgacccgaa gtatgccgcc gggtggagca tgaagtgcag
     3361 cgtcctctat catctcggca tgtaccgcca cgcgcttgcg tgtgccgaca aagcgcttgc
     3421 gatcaatcct tcctgcgagc tgaccaccca ggtccgtaag atgctcctct ccctcatcga
     3481 gaagtggtga gggggcccgc acgggacatt cgaaaggttg acgccgggcg ccgtccaacc
     3541 aagagaccgt gaggaaactc tcccccgggg gagactccga ccgtctatta cccggcggtg
     3601 ccaacaggtg aattgtatcc atgccggtcg atcagatccc tatcggtacg ttctcgcgga
     3661 tcacccatct atcgcagaag gcgctccgcc actacgacga gcggggactt ctcgtccctg
     3721 cggcgcgaga cgtgtgcacg gggtatcgct actacacctt tgcccaggcc gagcggggcc
     3781 tccgcatcag gcacctgctc tggctggggt tcgacctctc cgaggtcgag gcggttcttg
     3841 cggccaggga gagcggcgat gccggagcga tccgcgatct ctttcgcgcg cgcctcgctg
     3901 caaccgagcg ggaagctcta cggctgcatg cgattgcaga ggtcctgcga acacaggatc
     3961 ccctgaacgg aggattccat atgtcgatca ccgaacctgc catcaaggat gtccctgccc
     4021 agcgtgcgct cagcctccgg gagcggggta cctaccacga agcgatcccg agactgatcg
     4081 gggaactact cacgtatgtc tgtcccgaag acgggcggcg gcccgctgcc cggatggccg
     4141 ggccggtcat attcatctgc cacgacgagg aataccggga gacggatgcg gatatcgagg
     4201 tcgcgatccc gatcatcggg ccggtcagcc ttgaaaactc acgggtcgat atcgtgaccc
     4261 ttccggccgg ccggttcgtc tcggtgctcc acacgggccc ctatccgggt gtcgggaagg
     4321 cctacgagcg gctctttgcc tgcatgggcg agcgcggtct tatccccgcc ggcccctcgc
     4381 gggaactcta cctcaacaac cccggagagg tatcggagga cgagctcctg acggagatcc
     4441 agttcccggt gcaggagatc cctccctccc cctgacggga ggaggtcacc ctccctttgg
     4501 tatgatctcg aacaggtgga acccccaccc gggtatgtcc aggtagagcc cgggcgaaag
     4561 aaggctctcc ccgtcgcggt cgtatgctgc ctcccccatg aggtcgcgga gccggatgcc
     4621 tttccccgct atatcggccc acgggagcgg gacgtaacac tggcccctgt cgggcgcgta
     4681 gttgacggtt acgaggcagc gttctccctc cctctcccgg gcaaaggcga tgtagccgcc
     4741 gtgggacggg tttccttccc aggcggggtt gcagtcgagg agccgccaat ccccgtcccg
     4801 gaacgccggg agccggagag aggcagaaag ccgccggtag aactcttcta tggccggatc
     4861 gcccgggtcc tccggctccc ggcagatgtg gacgggagtc tgcctcgtgc gcccggcaaa
     4921 ctgcccctgg tgaaagaacc ggaggccggg cgagaggaag gcgaggacag cggcagcccg
     4981 gtggcggtcg ggcgaaaaga cggctgccgc ccggggttcg tcgtggttct cgaggaaatg
     5041 gacggattta tccaggtatg ccggatccgc ccggaggtgc tcccgcaccg accgtgcgtc
     5101 gccccaccgc agtcggtcgt agagccgttt gtcgtaggtg tagtcaaacc cctgctgctg
     5161 gagcgtccac tccaggtccc ggtagacctc cgccatgaag acgaattccg gaaggttctc
     5221 gcgaacagca ccgatcgctt tcggccagaa aggctccata tccctccccc aggtctgccg
     5281 gaagacttcc gggaggacga gcatcgccat gtcgcaccgg aggccgtcgc acttcctcgc
     5341 gatcccctgc acctcggcga tcatcgcttc ccgaagatcc gggttactgt aatcgagctg
     5401 cagggtgtcg ggccagccgc cggagaacgt gtcccggccg tgggcgagga cgatccctgg
     5461 gtccaccccg gcatagtcct gcggccggcg ggcgaggtct tcctccgtgc cgtggacgta
     5521 gtagtcgggg tgatcgcgca cccaggggtg atcgggcgcc gtgtggttcg ggacgaggtc
     5581 gagcatgagg aggaggtcgt gccggtgcag ccgttcgcgg aaccgctcga gcgtctccgg
     5641 ctctccgagg tccgggtgga ggcggtaggc ggcgacggca aaaggagagc cgcagatgtc
     5701 gtcctccccg atatcgggga tcaggctccg gtagccgctg agccactcct cgtgtgagcg
     5761 ggagacccgg agcccggcat ctcccgtcgt ccaggcgccc atgaggtaga cccaggagaa
     5821 tccgcaggcg gcaagccctg caagccaggt gtcggggata tcgtcgaggg tggcgcggcg
     5881 gccgatctgc cggacccgga cgcgggcgtt gacctcgtag agggaggggt agcggggggt
     5941 gttcatgcgg ggctctcttc ggtcctcggg acgatagagg tatccctcac cagacggcgg
     6001 agtggagatc gccgtcccgc cggaaccccc gaaacgaccg cgaaccctgg acggcatcga
     6061 tgacccgggt gctcgatgcc cgcatgaagg tcttgaggag cgcatgcgtc cgcccttcga
     6121 ggccgccgta gaggtagccc cggagcgccg cctccgcctg cagctccggt gtgaggcggc
     6181 ggtcgaggag ggcggtgtag gtctcgccgg ccggcgcacc ggacgtgagg tggcggtcga
     6241 cggcgtgggc ggcaagttct gcggtcctgt gggcgtaaaa gatcccttcg ccgaggatgg
     6301 ggcttgcaaa ccccccggca tcgccgacga ggagcgtccc ctcatgggca ggcgaggaga
     6361 tgaagtttcc aaacggcagg aagtatcccg ccggccgcct cttcgtgaac gccgagaggc
     6421 cgagggtctt gaggaactct gtaaatcggc cgcgctggtt gcctttgttc ttcgtgagca
     6481 gccctcccac ccccacgatg agcgcttccc tgttcgggaa gacccacccg tagccccacc
     6541 ggcaggcggc aaggacgatc tgcggcgtcg caaggtcggc gtcgaggtgg atcgcgccgt
     6601 cgccgtccgc gagcgtcttc acctccgttc gcgggatcgt gagttccaga gcccacccga
     6661 ggttctcctg ccatcgctgc cggtcgacga cgctctccgg gagggagcgc cggacccggc
     6721 tgttgatacc gtcggccccg atgaggaccc gggcggcgta tcggtccccg ctcgacgtcg
     6781 tgacggtgcg ccgggcgtgg tcgacggcga cgacctctgc gccttcgtag atatccgccc
     6841 cggcatccgc cgccatccgg gcgaggaagg catcgtagcg ctcccgccgg gtaaagtaga
     6901 gcggctccga aaggtccccg gcgaggaccc ggctatccct gatgtagagc gcatacccgg
     6961 tcccggtcga atcgagcagg cccgcttcgt ggagtgcggg gaccgggagg tcaaagaccc
     7021 gttcgaggaa ccgaatggac ttctggctga ggcagccccc gcagaccttc tcccgcggat
     7081 acgtctcttt ctcaaggagc gccacccggt gcccgaaccg cgcgagcagg caggcggcgg
     7141 tgctcccggc aggcccgccc ccgatgacga cgacatcgta tgccggttga tcctccatac
     7201 ttctctctcc tcggcgacga tagggtccgg cgcagactat attcctttcc ccggagggag
     7261 tatggtgtgc gccgcgattg aatagcgttt ctcaagaggg gtatttacgg gctccccttt
     7321 tgccgcgaag gctccctccg gaggctgctt tcctctcccc ggcagcagcg gggatatcgc
     7381 cggcccgtcg ttcggaacgg ggttctcgcg aatatccgcg cgtagccccc gggcgatcgc
     7441 cgccgaaggc cggttgtata acccggggta gagtagaaag aactgcacgc caggttccat
     7501 tcactttcac cccgcacccg acaggctcat cttctatcgg gccgatgctt ccggtatgtc
     7561 cgttgcggag cagaaactca gccatctcac cgctgccagc cggttcgacc tctgctgctc
     7621 cggggagtgt atccggacgc tcttcggtgc ttctcacatc acctacaacc cccgggccga
     7681 aggatgcgag cggatgctca aagttctcct ccacggcgcc tgctcctacg attgcgccta
     7741 ctgcgacgtc cgcctccaga gggagcgcct ctcgctctcc ccggatgaac ttgccggcac
     7801 cttcctcggt ttctggcggg aggggctcgt cgacggcctc ttcctgagct ccgggattcc
     7861 ccgcgacgtc gacctggtga tgcacgaact cgtggagacg ggagaaatcc tccgccggtg
     7921 cggctacgac ggctacctgc acttaaaggt cctccccggc gcggcccggg cggatatcca
     7981 cgacgccgcc cgcgtggcaa accggatcag catcaacctc gaggcgacgt ctcccgaccg
     8041 cctgggggga atcgccggcg tgaaggacta ccggcaggac atcctgaagc gccaggcctg
     8101 ggtggcggaa gagaaaccgg ggcatcacac cacccagctc gtcctcggcg cggcgggcga
     8161 gaccgatgcg gaggtcctct cctgcgtcgc cgaccagtac cggcgggtcc ggccttcgcg
     8221 gatctacttt gctgcatttc gcgctctgcc cgggactcct cttgcggtgc accccgatac
     8281 gccggcatgg cgggcgcggc ggtggtacca ggccgactac ctcctccggg actacggcgt
     8341 cacgcccgat accctccggg ttgctctcga cgaggagggg ttctttgcaa accgcgaccc
     8401 aaaagaggtc atcgcggccg ggaatgcgcc ggtgaacgtc aacaccgccc cgcgccgcgc
     8461 cctcctcatg gtccccggca tcgggcccaa gggggccgac cggatcctcg tgcaacggag
     8521 ggtgcggccc ttcaccggcc cggccgacct tgcccgggcc ggtatccggg gaaaggccgc
     8581 aggacggcac ctcacgttcg ggggagggga gacggttcag accaggctcg ttctgtaaaa
     8641 aggagggaag atcacctgat cttcccgaac tcctcgctca cttcggcggc ggagcggtag
     8701 gtccggtcgc cgaactgctc gatcacatcg atcacgctct ccggggcgcc gttctctttg
     8761 gcccgggcga ccaggtcctg tcttcccgcc gggtaatcca tccccctgag ataggactgc
     8821 aggtcggccg cggagaggtg gaccggggct ctcgtccggg tctcgggcgg tctctcgacg
     8881 gcggtctccc ggcgttctcc gtgggtcttg ctcccgaact cttcgctcac ctcggcaggg
     8941 gagcggtagg tccgatcgcc gaacccctcg aacgtcgcga tcacgctctc cggggcgttg
     9001 ttctttctgg cccggtcgat caggtcctgc cttcctgccg gataatccat ccccttgagg
     9061 tagacctgca ggtcggccgc ggagaggtgg gccggggcct tcatccgggt ctccgaccgc
     9121 tcctcgacga cggtggcccc cgcggttttc gtctccaccg tcggccgggt ctcttcggtt
     9181 ctcgtctccg ccgccgtgcc ggtcttctcg acctcttcgg cctttatcgg tttctcttcg
     9241 cgggaggcct tctcccgcac ctccacccgc tcggtcgtga cgacctcgcg gaacggaggc
     9301 tccgtccggg tcacggctcg cgcttcttcc tggctcggag ggcccacggg ccgcctcgcc
     9361 tcgatcaggt tccagtttgc ctctttcggc cactccccct ccgtaaaccc gggctcgttg
     9421 tcgagtctgt acttgcttat gttcaccaca aagacgtcgt cccccggccg gtagacgatg
     9481 gcctcgaggg ggatcgcgaa gtgtttcgcc ccgagaccga acatgccccc ggtcctgagc
     9541 acggcatacg ccacccttcc cgtgggcaga cctacccgga ggcttgagat ctcgccaagg
     9601 tcatacccct cggggttctt cacgcgcttc cccacgatat cctcggatgc gaggtactcc
     9661 tgtccctcgc ccctgcgcac ctgctgctcc agagttgcct ccgccattgc aatcaccatc
     9721 ttcgttgtct gctcccgaag gtgatcgcca ccttcgcgca gagcgggcga tgcgtgctga
     9781 gactatttaa cgtttccctg gttttcggga ggaatccctc cgcggcgaag aggacgtgcg
     9841 gggagagcgg gaaggccgtc tctccccatc cgccaaaaaa ttgcgggtat ttctgtcacc
     9901 tgaccttccc gaactcctcg ctcacttcgg cggcggagcg atacgtccgg tcgccgaact
     9961 gctcgatcac atcgatcacg ctctccgggg cgttgttctt cctggcccgg gcgac
//
LOCUS       DAOSII010000073         9895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_6615_length_9895_cov_46.328150, whole genome shotgun sequence.
ACCESSION   DAOSII010000073 DAOSII010000000
VERSION     DAOSII010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9895
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6615_length_9895_cov_46.328150"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /gene="rplF"
                     /locus_tag="PLV90_06650"
     CDS             <1..302
                     /gene="rplF"
                     /locus_tag="PLV90_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOF96833.1"
                     /translation="RGVVEGYEYRMKVVYSHFPIQLKLQGNRLEIGNFLGEKQPRAAK
                     IIEGVTVRIGNDEVTLTGIDREKVGNTAANIEHATKITKRDPRVFQDGIYITERA"
     gene            308..733
                     /locus_tag="PLV90_06655"
     CDS             308..733
                     /locus_tag="PLV90_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32e"
                     /protein_id="HOF96834.1"
                     /translation="MDETRRLIRARARHNKPAFKRRGLHRKAKLEDVWRRPRGLHSKQ
                     RRQFKAKGALPRPGYGSPAAVRGMHPSGYEEVRIFTPAELAGLNPESQAVRIAGAVGN
                     RKRAEIQTRALELGLKVLNAKDLVRAVEAPVEEEADEDE"
     gene            726..1178
                     /locus_tag="PLV90_06660"
     CDS             726..1178
                     /locus_tag="PLV90_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19e"
                     /protein_id="HOF96835.1"
                     /translation="MSDLANQKRMAAAILKCGVNRVWFDPERQTDIEDAISRSDLRTL
                     IEEGAIRAHAVKGNSRGRARARMAQRSYGHRKGPGRRKGAAGARASSKRTWIGKIRAQ
                     RRALRDMRGEGRIERSLYRVMYRRASGGQFRSVAHLEAQVEIAAGRMR"
     gene            1178..1705
                     /locus_tag="PLV90_06665"
     CDS             1178..1705
                     /locus_tag="PLV90_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOF96836.1"
                     /translation="MATGPRYFVPFRRRHEGKTDYYRRMSLLSSGSPRMVVRKTNRQI
                     IVQLVVPGNEGDRTLVAAYSAELAGYGYEGSTASTPAAYLTGMLFAVKALNAGYEEGI
                     LDIGLARAKSGARVFAALKGAVDGGLDIPYGESILPDEERLKGAHIAEYAPEQAGNLV
                     ANVEAVALAIKKELV"
     gene            1708..2325
                     /locus_tag="PLV90_06670"
     CDS             1708..2325
                     /locus_tag="PLV90_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOF96837.1"
                     /translation="MAYAQEEWIPLTGLGRMVAAGEITSIDEVLASGRPIKEPQIVDL
                     LLPDLEDEVLDINMVQRMTDSGRRVKFRTVVVVGNRNGYVGFGQGKDAQVGNAIQKAI
                     TDAKVNLIKVYRGCGSWECGCDTTHSIPIEVTGKAGSVRVTLKPAPQGIGLVTGDIPK
                     KVLTLAGIKDVWAFNRGQTRTTINYAKATFEALRQTNIVRIGGTE"
     gene            2326..2787
                     /locus_tag="PLV90_06675"
     CDS             2326..2787
                     /locus_tag="PLV90_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOF96838.1"
                     /translation="MYAVVQVRGVVNTNREIKDTLKMLRLHHVNHCVLVPDTPAYLGM
                     IRKVKDFVAYGEVDAGTLATLLRTRGRLTGDEKLTDEYVRAHTPYTGIDEFAAALCSG
                     ETAICDLAEIKPVLRLHPPRKGYKTIKRTFQQGGALGYYGSEINDLLYKMR"
     gene            2793..3218
                     /locus_tag="PLV90_06680"
     CDS             2793..3218
                     /locus_tag="PLV90_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL15m family ribosomal protein"
                     /protein_id="HOF96839.1"
                     /translation="MPVNKRTKYRGSRTCGGGTHKNRRGAGNRGGRGRAGQRDHRFSH
                     FYLKGEISNGKHGFISKTSVPVSALDVGEIDQMVEALLREGFASKEGDLIALDAAGLG
                     IDKVLGGGRVTHRMSISARAFSERARAKIEEMGGQVTTV"
     gene            3239..4672
                     /gene="secY"
                     /locus_tag="PLV90_06685"
     CDS             3239..4672
                     /gene="secY"
                     /locus_tag="PLV90_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOF96840.1"
                     /translation="MGDLLDRFEPFLAAMPAVRSPEGHVHFKNKLVWTLAILLLYFTL
                     TNISIFGLSPQSQDIFGIYRALLAGASGSLLHLGIGPIVTASIVLQLLKGAGILQIDT
                     SDARGQVMYMGLQKMLIFVMIIVEALPMVLSGMMMPDPSVATQFFGGNMFAVSLLIFL
                     QLCIGGLLVVLMDEVVTKWGVGSGVGLFIVAGVSQGLVNGFLNWQTGTDPFPIGFFPR
                     LFAIGTSGANFLEYFGTDVLALVTTIIIFLVIVYVESTRIEIPLAHTAVRGARARFPV
                     KLIYASVLPMILVRVLQANIQMIGMFLSNAGMTILGEFQGQHPVNGLMWYIAPINQPQ
                     DWMWWLADLGHAPWEILLRMGIDIAVMVLGGAIFALFWVKTAGLDSKDVARQIQRSGM
                     HIPGYRRNEQVLVRYLDRYIPRITIIGGAFIGVLSVVANLFGVVGAVGGTGLLLAVSI
                     TYRLYEEVASQQIMEMYPFMRGFFGKE"
     gene            4835..5410
                     /locus_tag="PLV90_06690"
     CDS             4835..5410
                     /locus_tag="PLV90_06690"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOF96841.1"
                     /translation="MGKKVVVTGVPGVGKTTVINGAMERLAAEGIAYEAVNFGSFMFD
                     VARKENLAADRDEMRKLGKDVQKRLQQAAASEIAAMSADANIIVDTHSSVKTPAGFLA
                     GLPEWVLKELMPDIVVLVETDPDQILMRRLGDASRTRDMEGARAIAEHQEFNRAVSAA
                     YAMYTGCTVKVVRNENYLLERGIDDLADVLR"
     gene            5417..6037
                     /locus_tag="PLV90_06695"
     CDS             5417..6037
                     /locus_tag="PLV90_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EMC3/TMCO1 family protein"
                     /protein_id="HOF96842.1"
                     /translation="MVDLKKHGPTIALLFTMLVMLSYGIEGVRVTVGTMMNVLLGPFI
                     DVLGVPFFVMIMILSSITGLYSSLVQKYTIDYEKMQETQAKMRVFQKEFREAQLSGDE
                     KRVKKLQGKQERMMQDQLDMSRQQFTPMAIILILSVPIFFWLLLRLPAVGTPIDIASG
                     IVMPFIGTVTLSSIAFWIVPAWILWYMLCSLTISQVIRKALNIGGL"
     gene            6037..6579
                     /locus_tag="PLV90_06700"
     CDS             6037..6579
                     /locus_tag="PLV90_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOF96843.1"
                     /translation="MRITISGPPGSGTTSLARYLAGKYDLAFISAGEVFRQLAREHDM
                     ELAEFGRFAENDPAVDRMIDARQKEIGEGSEKIIVEGRLSGRMIENADLRIWLSASIS
                     CRAKRIAGRDGMDEEEARVYTENRQRSEATRYRSYYGIDIGDLSPYDIVLSSETFGVD
                     ALAAIVDTAIACLTRQKGSL"
     gene            complement(6576..7220)
                     /locus_tag="PLV90_06705"
     CDS             complement(6576..7220)
                     /locus_tag="PLV90_06705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOF96844.1"
                     /translation="MPIDDIKPTRAGLLIVRRRMALAERVHELLSMKLDGMMLELIRL
                     TDRAAEQRRELEDLYTGAREMAAVAAMMEGATGVLLAALSVEAVPTYAVGHKNVFGVR
                     LPDLEPVAVKKTLDERGYGVLGTSSVIDDAADAYENLIEAIIASAELEGGVRQLLDEI
                     ERTRRRVNALEFKVIPELMEKRRFIEYQRDEMERQEWTRLRRIKKIKAKRAEKR"
     gene            complement(7230..8354)
                     /locus_tag="PLV90_06710"
     CDS             complement(7230..8354)
                     /locus_tag="PLV90_06710"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HOF96845.1"
                     /translation="MALRGMISPSYIYACTRFRVRRTKLLPRDAYRRIMQMGIPEIVT
                     HLGRQEEYQEIMDLAGDFSGARLIEEAVNRNLARSFRGAMAIAPGSLHTLTGEYLARW
                     DIANVMAILRGTVHHIPRQQIRDLLIPAGELDRALLDHLLSFTTCGEALDALQGWRLY
                     PILAEHYRVCGEEGAFARIEDELYQRYYAGLLGLARSGCSGCQELIPYLRFEIDITNM
                     KNLLRLHCAREACDITLVDQTMIPGGNMSIDLLRRLYGAGTEAEFTSIFLQTTIVPVL
                     ARAVRELRQDPNFSSTDAAELVWRRWHQHLRPVHEIEMAITRIRLQQMDALSRRYPFS
                     VLPVLAYLERKRYEVANLRAIARGKAFGLPADRIWQYIVL"
     assembly_gap    8526..8625
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8626..9674
                     /locus_tag="PLV90_06715"
     CDS             <8626..9674
                     /locus_tag="PLV90_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOF96846.1"
                     /translation="TRGRVRFISCADDSALIAMPYSIVTGDLFAGHDDPGHPESQARL
                     DAALAGVPSGARRIAPEQATIRDIERVHTGRHIEAIRTLCRACPPGRACYIDPDTYVT
                     GESFDAILYAAGAALQAAERALEGEHSFALVRPPGHHATPDRAMGFCIFNNVAVAAAK
                     ALLSVDRVAIVDWDVHHGNGTERIFFASDRVLYCSVHQAGLFPGTGRPDERGAGPGAG
                     YTVNAPLEAGSTGADYALVFRDLFIPAIRRFKPDLVMVSAGQDALAGDPLASMLLEPR
                     DFGALAGMLAGATPGALALVLEGGYGRLHAGAIGAIIAALDGARFVPGGGEPKAGTRK
                     LVMAHTGAGRAVPI"
BASE COUNT         1839 a         2972 c         3180 g         1804 t
ORIGIN      
        1 tccggggcgt cgtcgaaggc tacgagtacc gcatgaaagt ggtctacagc cacttcccga
       61 tccagctcaa actgcagggc aaccgccttg agatcggcaa cttcctcggc gagaagcagc
      121 cgcgggcagc gaagatcatc gagggcgtca ccgtccggat tggaaacgac gaggtgacgc
      181 tcaccggtat cgacagagag aaggtgggca acaccgccgc aaacatcgag cacgcgacga
      241 agatcacgaa gcgtgatccc cgggtgttcc aggacggcat ctacatcacc gagagggcgt
      301 gaagagaatg gatgaaacac gaagattgat ccgcgcccgt gcccggcaca acaaacctgc
      361 cttcaagagg cgaggactcc accgcaaagc gaagctcgaa gatgtctggc ggcggccgcg
      421 cggtctgcac agcaagcaga gacgacagtt caaggcaaag ggagcgcttc cccggccggg
      481 gtacgggagc cctgccgcag ttcgcggcat gcacccgagc ggctacgagg aagtccggat
      541 ctttacgccg gcagaacttg ccgggttgaa cccggagagc caggccgtcc ggatcgccgg
      601 agcggtaggc aacagaaagc gcgcagagat ccagacccgc gccctggaac tggggctcaa
      661 ggtactgaac gcaaaagatc tcgtccgtgc ggtcgaagcg ccggttgaag aggaggcgga
      721 tgaggatgag tgatcttgcc aaccagaagc ggatggcggc cgccatcctc aagtgcgggg
      781 tcaaccgtgt ctggttcgat cccgagcgcc agaccgatat cgaggatgcc atctcccgaa
      841 gcgacctgcg gaccctcatc gaggagggtg caatcagggc tcacgccgtg aaggggaaca
      901 gccgcggcag ggcgcgtgca cgaatggcac agcgttccta cggccaccgg aagggcccgg
      961 gtcggaggaa gggtgccgcc ggtgcccgtg catcgagcaa gcgcacgtgg atcggtaaga
     1021 tccgggcaca gcgccgggct ctgcgcgata tgcgcggcga aggcaggatc gagcggagtc
     1081 tctaccgcgt gatgtaccgg cgggcttccg gaggccagtt ccggagcgtg gcgcatcttg
     1141 aggctcaggt tgagatagcg gcagggagga tgagataatg gcaacaggcc cacgatactt
     1201 cgtgcccttc cgaaggagac acgaaggtaa gaccgattac tacaggcgga tgtcgctcct
     1261 ctcgtcaggc agcccgagga tggtcgtccg gaagacgaac cggcagatca tcgtgcagct
     1321 ggtcgtcccc gggaatgagg gggatcgcac cctggtggct gcgtactcgg ccgagcttgc
     1381 cggttacggc tacgagggat cgaccgcgag cactccggcc gcctacctga ccgggatgct
     1441 gtttgcggtc aaggcgttga atgcagggta tgaggagggt atcctcgata tcgggcttgc
     1501 ccgggcaaaa tccggggcgc gtgtctttgc cgccctcaaa ggagcggtgg acggcggcct
     1561 cgatatcccc tacggcgaat cgatcctccc cgatgaagaa cggctcaaag gtgcccacat
     1621 cgcggagtat gctcccgaac aggcgggcaa cctggtagcg aatgtagagg ctgtggctct
     1681 ggccatcaag aaggagctgg tgtgacaatg gcatacgcac aggaagaatg gattccgctc
     1741 accggtctcg ggcgcatggt cgccgcaggc gagatcacca gtatcgatga ggtgctcgcg
     1801 agcggtagac cgatcaagga gccccagatc gtcgatctcc tcctgcccga cctggaggac
     1861 gaggtgctgg acatcaacat ggtccagcgg atgacggact cgggtcgccg cgtaaagttc
     1921 cgcaccgtcg tggtggtcgg caaccggaac ggctacgtcg ggttcggcca gggcaaggat
     1981 gcccaggtcg gcaacgcgat ccagaaggca ataacggatg caaaggtgaa cctcatcaag
     2041 gtctaccggg gatgcgggag ctgggagtgc gggtgcgata ccacccactc gatccccatc
     2101 gaggtgaccg gcaaggcagg cagcgtccgg gtgacgctga aacccgcccc gcaggggatc
     2161 ggtctcgtga ccggggatat acccaagaag gtcctgaccc ttgcaggcat caaggatgtc
     2221 tgggcattca accgggggca gacccggacg accatcaact acgccaaagc gacgttcgag
     2281 gcgctccggc agacgaacat cgtcaggatc ggggggacgg agtaaatgta cgcggtagtg
     2341 caggtgcgcg gcgtggtcaa taccaaccgc gagatcaagg acaccttgaa gatgctccgt
     2401 ctgcaccacg tcaaccactg cgtcctcgtg cccgatacgc ccgcgtacct cggcatgatc
     2461 cgcaaggtga aagacttcgt ggcgtacggc gaggtggatg cagggaccct ggccaccctt
     2521 ctccgcaccc ggggcaggtt gaccggcgac gagaaactga ccgacgagta cgtccgtgcg
     2581 catacgccgt ataccggtat cgatgagttt gcggcggcac tctgcagcgg tgagaccgcc
     2641 atctgcgatc tcgccgagat caagccggta ctgagactgc acccaccacg aaagggctat
     2701 aaaactatca agcgaacctt tcaacagggt ggagctctcg gctactacgg atccgagatc
     2761 aacgatctcc tctataagat gaggtgagac tgatgcccgt aaacaagaga accaaataca
     2821 ggggttcacg gacctgtggc ggcggcacgc acaagaacag gcgcggtgcc ggcaacaggg
     2881 gtggacgggg tagagccggg cagcgggatc accgcttctc ccacttctac ttgaagggcg
     2941 agatatccaa cggcaagcac gggttcatca gcaagacgtc cgtgccggta tccgccctcg
     3001 atgtcgggga gatcgaccag atggtcgaag cgctgctccg cgaggggttt gcaagcaaag
     3061 agggcgacct cattgccctt gatgccgccg ggcttggtat cgacaaggtg ctcggcggcg
     3121 gaagagtcac ccacaggatg agtatatccg cccgggcgtt ctcggaacgt gcccgggcta
     3181 aaatcgagga gatgggcggt caagtaacga ccgtctgatc ttcttttcag gtgagaccat
     3241 gggagatctg ctggatagat ttgaaccctt ccttgcagcg atgcctgcag tccgaagtcc
     3301 cgaggggcac gtccacttca agaataagct ggtgtggacg cttgcaatcc tgctcctcta
     3361 cttcacattg accaacatca gtatctttgg actctctccg cagtcacagg atatcttcgg
     3421 aatataccgt gccctgcttg ccggtgcaag cggttcgctc ctgcatctcg gtatcgggcc
     3481 gatcgtcacc gcgtcgatcg tgctgcagct gctcaagggc gccggaatcc tgcagatcga
     3541 tacaagcgat gcccgcggcc aggtcatgta catgggcctg cagaagatgc tcatcttcgt
     3601 gatgatcatc gtcgaggcgc ttcccatggt cctgagcggt atgatgatgc ccgacccgtc
     3661 ggtagcaacg cagttcttcg gcgggaatat gttcgcggtc tcgcttttga tcttcctcca
     3721 gctctgcatc ggcggtcttc tcgtggtgct gatggacgag gtcgtcacca agtggggtgt
     3781 cggttcgggc gtggggctct tcatcgttgc gggggtctcg cagggtctcg tgaacgggtt
     3841 cctgaactgg cagacgggca ccgacccctt cccgatcggg ttcttcccgc gtctgttcgc
     3901 catcggaacg tcgggggcga acttcctcga atacttcggc acggatgtgc tcgccctcgt
     3961 cacgacgatc atcatcttcc tggtcatcgt ctacgtggag tcgacccgca tcgagatccc
     4021 gcttgcacat accgccgtcc ggggcgcccg cgcgcggttc ccggtgaaac tcatctatgc
     4081 gagcgtcctc cccatgatcc tggtccgggt gctgcaggca aacatccaga tgatcggtat
     4141 gttcctctcg aacgccggta tgacgatcct cggcgagttc caggggcagc atccggtaaa
     4201 cggcctgatg tggtatatcg ctcccatcaa ccagccacag gactggatgt ggtggcttgc
     4261 cgacctcggg cacgccccct gggagatcct cctccggatg ggtatcgata tcgccgtcat
     4321 ggtgctcggg ggtgcgatct tcgcgctctt ctgggtcaag accgcgggtc tcgactcaaa
     4381 ggacgtggcc cggcagatcc agaggagcgg gatgcatatc cccggttatc gccggaacga
     4441 gcaggtgctt gtgaggtatc tcgaccgcta cataccgagg atcaccatca tcggcggtgc
     4501 gttcatcggg gttctctcgg tcgtcgcaaa cctcttcggt gtggtcggcg cggtcggcgg
     4561 aacgggtctg ctgcttgcgg tgagcatcac ctaccggctc tacgaggagg tcgcgagcca
     4621 gcagatcatg gagatgtacc cgttcatgcg gggattcttc gggaaggagt gagtccgccc
     4681 ggactcacct ttctttgaaa tattgccgtt acatcctttt ttttgtcccg ggcgagtccg
     4741 gccgccggtc cgggtcgggt tgattccgaa aggcattact tccgtgagcg cggatttatc
     4801 ctgcagggaa gataacataa gtggagtgat agagttgggg aagaaagtcg tcgtaacggg
     4861 ggtccccggt gtcgggaaga ccaccgtcat caacggtgcg atggagcgcc tggcggccga
     4921 aggtatcgcc tacgaggccg tcaacttcgg ttcgttcatg ttcgacgtgg cgaggaaaga
     4981 gaacctcgcc gcagatcgtg acgagatgcg taaactcgga aaagatgtcc agaagcgcct
     5041 tcagcaggcg gcggcgtcgg agattgccgc catgagcgcc gatgcgaaca tcatcgtcga
     5101 tacccacagc agcgtcaaga ccccggcggg gttcctcgcg ggactgcccg agtgggtgct
     5161 caaagagctg atgccggata tcgtcgtgct ggtggagacc gatccggacc agatcctgat
     5221 gcgccgtctc ggcgacgctt cgaggacccg ggatatggag ggagcgagag cgatcgccga
     5281 gcaccaggag ttcaaccgcg cggtcagtgc ggcgtatgcg atgtataccg gttgcaccgt
     5341 caaggtcgtc cgaaacgaga actacctcct cgagcggggc atcgacgacc tggcagacgt
     5401 gctgaggtaa cctgacatgg tggacctgaa gaagcacggc ccgacgatcg ctctcctctt
     5461 caccatgctc gtgatgctct cctacggcat cgagggggtg cgggtgacgg tcggcacgat
     5521 gatgaacgta ctgctcgggc cgttcatcga cgtcctcggc gtgccgttct tcgtcatgat
     5581 catgatcctc tcgagcataa cgggcctcta ctcgtcgctc gtccagaagt acaccatcga
     5641 ttacgagaag atgcaggaga cgcaggcgaa gatgcgggtc ttccagaagg agttccgcga
     5701 ggcccagctc tccggggacg agaagagggt caagaaactc caggggaagc aggagcggat
     5761 gatgcaggac cagctcgata tgtccaggca gcagtttact ccgatggcga tcatcctcat
     5821 actctcggtg cctatattct tctggctcct cctgcggctc ccggctgtcg gcacgccgat
     5881 cgacatagcg agcggtatcg tgatgccctt catcgggacc gtcacgctct cgagtatcgc
     5941 gttctggatc gtgccggcct ggatcctctg gtacatgctc tgctcgttga cgatcagcca
     6001 ggtgatccgg aaagcgctca acatcggggg actctgatgc ggatcaccat cagcggccct
     6061 cccgggagcg gcaccacgtc gcttgcccgc tacctcgccg ggaagtacga ccttgccttc
     6121 atatcggcag gggaggtctt ccggcagctt gcccgggagc acgatatgga acttgccgag
     6181 tttggccggt ttgcggagaa cgaccccgcg gtcgaccgga tgatcgacgc ccggcagaaa
     6241 gagatcggcg aaggatccga gaagatcatc gtcgagggca ggctctcggg ccggatgatc
     6301 gagaacgcag acctccgtat ctggctttct gcgtcgatat cctgccgggc aaagaggatt
     6361 gcggggcgtg acggtatgga tgaggaggag gccagggtct acaccgagaa ccggcaacgc
     6421 tcggaagcca cccgctaccg gagttactac ggcatcgata tcggcgacct ctcgccgtac
     6481 gatatcgtcc tctcatccga gacctttggc gtggacgctc ttgccgcgat cgtggatacg
     6541 gcgattgcgt gccttacgcg gcagaaaggc tctctctagc gtttttccgc cctctttgcc
     6601 tttatctttt tgatacgccg gagccgcgtc cactcctgcc gctccatctc gtcgcgctgg
     6661 tactcgatga accggcgttt ctccatgagc tccgggatga ctttgaactc cagggcgttc
     6721 accctccggc gggtccgctc gatctcgtcg aggagctgcc tgacgcctcc ttcgagttcg
     6781 gcgcttgcta tgatcgcttc gatgaggttc tcgtaggcat cggcggcgtc gtcgatgacc
     6841 gatgatgtcc cgaggacgcc gtatccccgt tcgtcaagcg tcttcttcac cgctaccggt
     6901 tccaggtccg ggagccgtac gccaaagacg ttcttgtgcc cgaccgcgta ggtcggtacg
     6961 gcctccaccg agagcgcggc aaggagcact cccgtcgctc cctccatcat ggcggcgacg
     7021 gctgccatct cccgggcccc ggtatacagg tcttcgagtt cccggcgctg ttctgcggcc
     7081 cggtcggtga gcctgatcag ctccagcatc ataccgtcga gtttcatcga gagcagttca
     7141 tgtacccgct ccgcgagcgc catccgccgc ctgacgatca ggaggccggc ccgggtaggt
     7201 ttgatgtcgt cgatgggcat gatccttcct cacagcacga tatactgcca tatccggtcg
     7261 gccggcaggc caaacgcctt cccccgcgcg atcgcccgca ggttcgcgac ctcgtatctc
     7321 ttccgttcaa ggtaggcgag caccggcagg accgagaacg ggtagcgccg ggagagggca
     7381 tccatctgct ggagcctgat acgggtgatc gccatctcga tctcgtgaac gggccgcagg
     7441 tgctggtgcc atcgccgcca gaccagttct gcggcgtccg tgctcgagaa gttcgggtcc
     7501 tgccggagtt cgcgcaccgc ccgggccaga accgggacga tagttgtctg cagaaaaatg
     7561 ctggtgaact ctgcctctgt cccggcgccg tagagccggc ggaggagatc gatggacatg
     7621 ttgccgcccg ggatcatggt ctggtcgacg agggtgatgt cgcacgcttc ccttgcacag
     7681 tggagccgga ggaggttctt catgttggtg atgtctatct cgaaccggag gtaggggatc
     7741 aactcctggc aaccgctgca ccctgatctg gcaagaccga gcagcccggc gtagtagcgc
     7801 tggtagagtt cgtcctcgat ccgggcaaag gcaccttcct ccccgcagac ccggtagtgc
     7861 tctgcaagga tcgggtagag tcgccagccc tgaagggcat cgagcgcctc gccgcaggtc
     7921 gtgaaggaga ggaggtggtc gaggagcgcg cgatcgagtt cgcctgccgg tatgaggagg
     7981 tcgcggatct gctgcctggg tatgtggtga accgtgcccc gcaggattgc catgacgttt
     8041 gcgatgtccc accgggcgag gtattctccc gtcagggtgt ggagactccc gggagcgatc
     8101 gccatggcac cccggaacga tcgcgccagg ttgcgattaa ccgcctcttc gatgagccgg
     8161 gcgccggaaa agtcgccggc gagatccatg atctcctggt actcctcctg ccggccgagg
     8221 tgggtgacga tctccggtat gcccatctgc atgatccggc gatacgcatc gcgggggagg
     8281 agctttgtcc tgcggacgcg gaaccgggtg caggcgtaga tatacgacgg agagatcatg
     8341 ccgcgcaggg ccatgtacca ctattatcgt cccacgtata aatgcgcacc cggccgatcg
     8401 ggggcgggag gtccggcagg ggcacatcct ggccctgccg gcgaatcgga gggcggcatc
     8461 tgccgggtgg ctcccgggtc ttgaagagca gcggcgatag cccggcccgg atacccgcgg
     8521 ccgggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatacc cgcggccggg
     8641 taaggttcat ctcctgtgcc gacgattctg ccctgatcgc catgccgtac tcgatcgtga
     8701 cgggagatct ctttgccggc cacgacgacc ccggccatcc cgagtcacag gcacgcctcg
     8761 atgccgcgct tgccggggtg ccgtccggcg cccgccgcat agccccggag caggcgacga
     8821 tccgggatat cgaacgggtg cacaccgggc ggcatatcga ggccatccgc acgctctgcc
     8881 gggcctgccc cccgggccgg gcctgttaca tcgacccgga cacctacgtc accggcgaat
     8941 cgttcgacgc gatcctctac gcggccggag ccgctctcca ggcagcggag cgggcgctcg
     9001 agggagagca ctctttcgcc ctggtccgcc cgccgggcca ccacgccacg ccggaccggg
     9061 cgatggggtt ctgcatcttc aacaacgtcg ccgtcgctgc ggcgaaagcg cttctgtcgg
     9121 tcgaccgggt tgccatcgtc gactgggacg tgcaccacgg caacgggaca gagaggatct
     9181 tcttcgcgtc ggaccgggtg ctctactgtt ctgtccacca ggcggggctc ttcccgggga
     9241 caggccggcc ggacgagcgg ggtgccggcc cgggtgccgg gtataccgtc aatgcaccgc
     9301 ttgaagcggg ctcgaccggc gccgactacg ccctggtctt tcgcgatctc ttcataccgg
     9361 ctatccggcg cttcaagccg gacctcgtga tggtctcggc ggggcaggat gcgctcgccg
     9421 gggacccgct cgcctcgatg ctcctcgaac cccgcgactt cggggctctt gccgggatgc
     9481 ttgcgggcgc aacacccggt gctctcgccc tcgtgctcga gggaggttac gggcggttgc
     9541 atgccggggc catcggggcg atcattgccg ccctcgatgg agcgcgtttc gtcccgggcg
     9601 gcggtgagcc gaaggcaggc acccgaaagc tcgttatggc gcataccggt gcggggcggg
     9661 ccgttcctat ctgacctgca cccaccggaa tcatcctccc tcccggcgaa tggtgcgcgc
     9721 ccctgctcgc ctcgcttgcg gcaagccttc ttgctctcac cctcctcacc atccggagcg
     9781 gggagaccct tggttgcctg gttccgcagt ctgaaggaaa gcggatccac gaggcgcagc
     9841 tcgcccgggc ctacgacccg gggagaggca tctccgggaa ggtccggggc ctctt
//
LOCUS       DAOSII010000074         9415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_6994_length_9415_cov_40.637927, whole genome shotgun sequence.
ACCESSION   DAOSII010000074 DAOSII010000000
VERSION     DAOSII010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9415
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6994_length_9415_cov_40.637927"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1989)
                     /locus_tag="PLV90_06720"
     CDS             complement(<1..1989)
                     /locus_tag="PLV90_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOF96847.1"
                     /translation="MKPQHGIVLVLLIGLIAGAAAAGDLPPALPHEFYGNVAIDGSPA
                     PAGTVVTAMIGGTACGSVEVTSPGRYGDPDWNLGNRLLVTGTEGQNGETITFLVDGGA
                     ADQTAPFTPGRVTRLDLSFEETVTVPTARFTANVTSGPAPLAVRFADASTGDPDSWFW
                     DFGDGENSAERSPTHTYTTPGTYTANLTVANAAGSNSATATIIVQEAGAVEIVRGPYF
                     TGTTTTATFVNWMTQEPVAGTVEYADDAYYAANGGYALSVADTAETAFHRVALEDLTP
                     GTLYHYRIVAGNTTLGDRTFRTFPEDGGFTFIVYGDTQLPANIKIVADRIADEDPLFI
                     LHTGDQVNAVESVDEWNNFFSSNRRLLANTTIYTTLGNHEYNDTVYYETFGLPQWYSF
                     VCADARFAVLDDNFASNPLPETAWLARDLTNDTAWKFVANHHPPYSSTSDRAGGWLYP
                     RAWWGQIMQDNGVTAVFNGHVHAYERYIVDGITYLVTGTGAGSLHPLGPANERPAGYQ
                     NSLVHTLGYTKITVHDNGTATGQFVQVARLDPNNSSQVLEVYPPGTAFETYTLTPRAR
                     GADLAAVSLDVPAGIVAGNACTVNGTVENLGGTVAGACPVTLAADGDLIGNLTVDPLA
                     PGETAEVSFTWTPAAAGEAVLTLSVDPASTVPDIDRTNN"
     gene            complement(1994..5506)
                     /locus_tag="PLV90_06725"
     CDS             complement(1994..5506)
                     /locus_tag="PLV90_06725"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOF96848.1"
                     /translation="MKQHRLVTITLMVLLLVAALAAPVLAAGTTEVRIAKFASDGKTV
                     LNETTVDYHWLEANLPVQGDGVTRYYHQGPVFEGIWEDAHPDKPYDGWNPDEDVQMSM
                     LVKADLGAVMGTDLRDICDYIGGAEEGDEIAVRSSDGWQSKRYPYEYIYDPDPRQGPA
                     VLCWFSGNSSGPDAQEAQGQGYPDTGYAVGMRMIFFADDTTNPWGWHIFGNNDMKECW
                     DEEYWNYGAQYPSAAGTSGKLVNEIRIYSQEEPPEPPIADFSVDIASGDAPLVVAFTD
                     CSEGNPTSWSWTFGDGATSAEQNPVHTYIIPGVFTASLTVTNAAGSDTAEFSIIVSGE
                     SPGVLHIVKYANDNATVLGERTVDYHWLAANLPVQGDGVTRYYHQGPVTEAAWKEVFP
                     YQAYKPWDPNERIESAFLDLGAVKGTDLAEICELVGGANDGDLVTLVADGAALTLPYG
                     TVYEPNPRQGPAVLCWYREGEGYLPDLLLAYFADNSTNLKAWHVFGNTDMQTCWSEAY
                     WTYNGTLPSAASSSRDRVTEVRIESQEPPAPPVPSFSTNVTTGTPPLAVAFNDTSTGA
                     PTAWSWDFGDGKTSTERNPVHVYAAPAVYPGIYTVTLTATNDVGTNITTVENCITVTA
                     GYASFTANTTGGLPPVAIRFTDTSAGNPTSWLWDFGDGATSTDQNPVHTYTSVGTYPV
                     TLTVTGSFGSDTYVTTEPIDMIRVLVPGDIGTTTLRVAKVAKNGTVLNETTVDYHWLE
                     ENLPVQGDGVTKYYHQWWIFDENPAYPDPDEANWNSVFNGKGRFGAVKGTDIKDICDL
                     VGGASEGDLIRLNSRDGYKTGLQYDWVYNPHPRQGPATLCWYNAGGGNDQGVGYPDTG
                     YFPSMRLIFFADNSTNPWGLHIFGIYDAYLAWGTWEDATTGWSAKWVDAVVIEVDFYT
                     STPVANFTADVTTGDAPLTVQFTDASTGGPDSWSWDFGDGNTSTDRNTTHTYTAPGTY
                     TANLTVENSAGSDSATATITVIEPGEPGETGTLTLHPGWNFVSTPKRLVNGANTFAIF
                     KDVDTADHAVLLYDGITGWRSANLTTPFRPLDGAWVYANETCSIPLALAADGPELPPE
                     KDLAKGWNAIGFTGTDPRTAKETLLSLGDSWTTLIGFDAQDQEYGVSILRGASGRHGE
                     ERPMYPMRGYWLYMTDAETLGAVG"
     gene            complement(5625..>6320)
                     /locus_tag="PLV90_06730"
     CDS             complement(5625..>6320)
                     /locus_tag="PLV90_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96849.1"
                     /translation="GSDNTGGSGNTGGTGSTGNTVTARESAAPTAAPEVQTGQASLAT
                     SASGEVLETVAVRTAGGTGTVTIPEGTTALDAGGNPLGEVTCREVAPAEVPAAPPGTT
                     VAVALNCGPAGATFDPPVVLTYTLSAEEWAEIGSATPKVIWYNPGTGKWQEIPATVDP
                     AARTITAEVAHFSIYALAWTVPATTAEAQQTGSPGTPTPTGGEPPWPLIGIGAVILIG
                     ATIGGFVYLRKKD"
     assembly_gap    6321..6420
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6421..6841)
                     /locus_tag="PLV90_06735"
     CDS             complement(<6421..6841)
                     /locus_tag="PLV90_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96850.1"
                     /translation="MRQGITLILLLGLVAGAAAAGEGTPTLPHGFSGGVTIGGSPAPV
                     GTVITATIGGTECGSFQTTETGEYGNPGRSVGDRLLVKATADQSGETITFYVDGVAAQ
                     ETSVFAIGGGTVLDLSIPGSGGSGNTDNTGGSDNTGGS"
     gene            complement(6861..>9415)
                     /locus_tag="PLV90_06740"
     CDS             complement(6861..>9415)
                     /locus_tag="PLV90_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOF96851.1"
                     /translation="VTVYADEPIVVNGTTYGGNSFFNILAAAGFTYTYAESDAGYGDR
                     KPLLTLTIDGVTYPTEDFGPDYKWKFYDGLGSSAYKYKKALNGYIDNSGTYYIWFGDT
                     SQFGSLDDPTVENSIYVLELNVTLLPTRPAEPGAPVADFTANVTSGELPLAVAFTDAS
                     TGAPTTWAWDFENDGTADSTEQSPVHVYPAAGTYTVNLTVGNSYGESSKLAEITVTKP
                     DPIVFNTAVTVYADEPIVVDGTTYDGNTFFNILAAAGYTYTYAESDAGYTDRKPLLTL
                     TIDGVTYPTEDFGPDYAWKFYETLGTGNDVKFKKALNGYISKSGTYYIWYGDMSQFDW
                     WELPSVENSTYVVELNVTLLPTRPGEDGTPAGVTIISPLPNSGYAVGASVRLAATATG
                     DNLTYTWDLGNGDTVVGQDTSYAYPAAGNYTITLTVANDAGSAEASTGITILDSDLPY
                     IVLYQGTVDIPAGSTIQAAISPRSGGYVPGEDFVEIPAESVGGMLNASGFIFNLWKST
                     KPIPTYSLTDITTPDGVYYPNVQDATANLAWRSFDGMTSAATKYDPDVPLANGTTIYT
                     VYGDYDVVNSDPSGARYVIITTLNIVEPGTANPPVAGFDASTTSGDAPLAVAFTDRST
                     NAPTSWSWTFGDGATSAEQNPVHTYTAPGTYTVSLMVANTAGSDSATKTIVVTEPGEE
                     ADTIVLRPGWNFVSTPKRLADGANTFAIFDGVDTAGRSILLYDGLDYDWRQMGLTDTF
                     RPLDAVWIYANGTSEIPLTFAGGAAELPPQKDLGKGWNAIGFSDTVPESAAATLLSLG
                     NSWATLIGYDAVDQRYESAIIRTTAGETREMQPMQGYWIYTTRAETLAAISG"
BASE COUNT         1362 a         2716 c         3427 g         1810 t
ORIGIN      
        1 gttgttcgtg cggtcaatgt ccgggaccgt cgatgccggg tcgaccgaaa gggtgaggac
       61 cgcttcgccg gcggctgccg gggtccaggt gaaagagact tctgcggtct ctccgggagc
      121 aagcgggtcg acggtgaggt tgccgatgag gtcgccgtcg gcggcgaggg tgaccgggca
      181 tgcgccggcc acggtgccgc cgaggttttc aacggtcccg ttgacggtgc aggcgttccc
      241 ggcgacgatg ccggccggga cgtccaggga gacggccgcg aggtcggcgc cccgggcccg
      301 cggggtcagg gtgtaggtct caaaggcggt gcccggcggg tagacctcga gcacctgact
      361 gctgttgttg gggtcgagcc tggcgacctg gacgaactgg cccgtggcgg tcccgttgtc
      421 gtggacggtg atcttcgtgt agcccagggt atggacgagg ctgttctggt agccggccgg
      481 cctctcgttg gccggcccga gcgggtggag cgagccggcg ccggtgccgg tgacgaggta
      541 agtgatgccg tcgacgatgt agcgctcgta ggcgtggacg tggccgttga agacagcggt
      601 gacgccgttg tcctgcatga tctggcccca ccacgcgcga ggatagagcc atcccccggc
      661 gcggtcgctc gtggacgagt aggggggatg gtggttggcg acgaacttcc aggccgtgtc
      721 gttggtgagg tcccgggcga gccaggcggt ctccgggagg gggttgctgg cgaagttgtc
      781 gtcgaggacg gcgaagcggg cgtccgcgca gacgaaggag taccactggg gcagcccgaa
      841 ggtttcgtag tagacggtgt cgttgtactc gtggttgccg agggtggtgt agatggtggt
      901 gtttgcaagc agccgccgat tgctcgaaaa gaagttgttc cactcgtcca cgctctcgac
      961 ggcgttgacc tggtcgccgg tgtggaggat gaagagcggg tcttcgtcgg cgatccggtc
     1021 ggcgacgatc ttgatgttcg ccggcagctg cgtgtcgccg tagacgatga aggtgaagcc
     1081 gccgtcctcg gggaacgtcc ggaaggtgcg gtcgcccagg gtcgtgttgc cggcaacgat
     1141 acggtagtgg taaagggtgc ccggggtgag gtcctcaagc gcgacgcggt ggaacgcggt
     1201 ctctgcggtg tcggcgacgg atagcgcgta tccgccgttc gcagcgtaat aggcgtcgtc
     1261 tgcgtactcg acggtgcccg cgacgggttc ctgcgtcatc cagttgacga acgtcgccgt
     1321 cgtcgtggtg ccggtgaagt acgggccgcg gacgatctct accgcgcctg cctcctgtac
     1381 gatgatggtt gccgtcgcgc tgttgctccc ggcagcgttc gcgaccgtga ggttcgcggt
     1441 gtaggtgccg ggtgtcgtgt aggtgtgggt cgggctccgt tcggcggagt tttcaccgtc
     1501 gccgaagtcc cagaaccagg agtccgggtc gccggtcgag gcgtcggcga accggacggc
     1561 gagcggcgcc ggcccggagg ttacgttcgc cgtgaacctc gcggtcggca ccgtcacggt
     1621 ctcctcgaac gaaaggtcga ggcgggtcac cctgccgggg gtgaacggag cggtctggtc
     1681 cgccgcccct ccgtcgacga ggaatgtaat cgtctcaccg ttctggccct cggtcccggt
     1741 cacgagcagg cggttcccca ggttccagtc gggatcgccg tatcttccgg ggctcgtcac
     1801 ctcgacggag ccgcaggcgg tacctccgat catcgcggtg acgacggtcc cggcaggtgc
     1861 gggagagccg tcgatcgcca cgttcccgta gaactcatgg gggagggcgg ggggtagatc
     1921 cccggccgcc gccgccccgg cgatgaggcc gatgaggagg accagtacaa tgccgtgttg
     1981 tggtttcacc ttctcacccg acggcaccga gcgtctccgc gtcggtcatg tagagccagt
     2041 aaccccgcat gggatacatc gggcgttctt caccgtgacg gccgcttgcg ccgcggagga
     2101 tcgaaacgcc gtattcctga tcctgtgcgt cgaaaccgat gagcgtggtc cagctatcgc
     2161 cgagcgagag cagggtctcc tttgcggttc tggggtcggt tccggtgaag ccgatcgcgt
     2221 tccagccttt tgcaaggtct ttttcaggcg ggagttcggg accgtcggcg gcgagggcga
     2281 gcgggatcga gcaggtctcg tttgcgtaga cccaggcccc gtcgagcggc cggaagggtg
     2341 tcgtcaaatt ggccgatctc cagccggtta tgccgtcgta gagcaggacc gcgtgatcgg
     2401 cggtatcgac gtccttgaag atcgcaaagg tgtttgcacc gttaacgagg cgcttcgggg
     2461 tcgagacgaa gttccagccg gggtggaggg taagggtgcc cgtctccccg ggctcgccgg
     2521 gttcgatcac ggtgatggtc gcggtcgcgc tgtcgctccc ggcagagttc tcgacggtga
     2581 ggttcgcggt gtaggtgccg ggggcggtgt aggtgtgggt cgtgttccgg tcggtcgaag
     2641 tgttcccgtc accgaagtcc caggaccagg agtccgggcc gcctgtcgag gcgtcggtga
     2701 actggacggt gagcggggcg tcgcccgtgg tcacatcggc ggtgaagttc gcgaccgggg
     2761 tggaggtgta aaaatctacc tcgatgacga ccgcatcgac ccactttgcc gaccagcccg
     2821 tggtagcgtc ttcccacgtg ccccaggcca ggtaggcgtc gtagatgccg aagatgtgca
     2881 gcccccaggg gttcgtggag ttgtcggcga agaagatcag gcgcatgctc gggaagtagc
     2941 cggtatcggg gtagccgact ccctggtcgt tgcctccgcc tgcattatac cagcagaggg
     3001 tggccgggcc ctgacgcggg tgggggttat agacccagtc gtattgaaga cctgttttat
     3061 agccgtccct gctgttcagg cggataagat ccccttcgga agctcccccg accaggtcgc
     3121 agatgtcctt gatgtcggtg cccttcacgg cgccgaaccg tcctttgccg ttgaagacgc
     3181 tgttccaatt cgcctcgtcc gggtccgggt aagccggatt ctcatcgaag atccaccact
     3241 ggtggtagta cttggttacg ccgtcgccct ggacgggaag gttctcctcg agccagtggt
     3301 agtcgacggt cgtctcgttc aatacggtgc cgtttttagc cacctttgca acgcgcaggg
     3361 tggtggtccc gatgtcgccc gggacaagca cccggatcat atcgatgggt tcggtcgtca
     3421 cgtacgtgtc gctcccgaag gagccggtga cggtgagggt gacggggtag gtaccgacac
     3481 tggtgtaggt gtggaccggg ttctgatcgg tcgaggttgc accgtcgccg aagtcccaga
     3541 gccaggaggt cgggttgccg gcggaggtgt cggtgaaccg gatggcaacc ggcggcaacc
     3601 cgccggtcgt gttcgcggtg aagctcgcgt atcctgcggt gacggtgatg cagttctcca
     3661 cggtggtgat gttggtgccg acgtcgttcg tcgcggtgag ggtgacggtg tagatgcccg
     3721 ggtagacggc gggggcggcg tagacgtgga ccgggtttcg ttcggtcgag gtcttaccgt
     3781 cgccgaagtc ccaggaccag gccgtcggcg ctcccgtgga ggtgtcgttg aaggcgaccg
     3841 cgagaggcgg cgttccggtg gtcacgttgg tgctgaagga ggggaccggg ggcgcggggg
     3901 gctcctggct ctcgatccgc acctcggtga cccggtcccg ggaggaggag gcagcggacg
     3961 gcagggtgcc gttgtaggtc cagtaggcct cgctccagca ggtctgcatg tcggtgttgc
     4021 cgaagacgtg ccaggccttg aggttcgtgg agttgtcggc gaagtaggcg aggaggaggt
     4081 cgggcaggta tccttcgccc tcacggtacc agcagaggac cgccgggccc tggcgcgggt
     4141 tgggctcgta gaccgtccca tacgggaggg tgagcgcggc gccgtccgca acgagggtga
     4201 cgaggtcgcc gtcgttcgcc ccgccgacga gttcgcagat ctcggcgaga tcggtgccct
     4261 tcacggcgcc gaggtcgagg aaggcgctct cgatccgttc attggggtcc cagggcttgt
     4321 aggcctggta ggggaagacc tccttccagg ccgcctcggt gaccgggccc tggtgataat
     4381 agcgggtgac gccgtcgccc tggaccggga ggtttgccgc cagccagtgg tagtcgaccg
     4441 tcctctcgcc caggacggtg gcgttgtcgt ttgcgtactt cacgatgtgg agcacgccgg
     4501 ggctctctcc ggagacgatg atcgagaatt ccgccgtgtc gctgcctgcg gcgttcgtga
     4561 ccgtcaggct cgcggtgaag acgccgggga ttatgtaggt gtggacgggg ttctgctcgg
     4621 cggaggtcgc gccgtcgccg aacgtccagg accaggaggt cgggttgcct tcggagcagt
     4681 cggtgaacgc gaccacaagc ggggcgtcac ccgaggcgat gtcgacggag aagtcggcga
     4741 tcggcggttc cgggggctct tcctggctgt agattcggat ctcgttgacc aacttgccgg
     4801 aggtgccggc ggccgagggg tactgcgccc cgtagttcca gtactcctcg tcccagcact
     4861 ccttcatgtc gttgttcccg aagatgtgcc agccccaggg gttcgtggtg tcgtcggcaa
     4921 agaagatcat gcgcatgccg acggcatacc cggtgtccgg gtagccctgg ccctgcgcct
     4981 cctgggcgtc ggggcctgat gagttgccgc tgaaccagca gaggaccgcc gggccctggc
     5041 gcgggtcggg atcgtagatg tactcgtagg ggtagcgctt gctctgccac ccgtcgctgc
     5101 tgcggacggc gatctcgtcg ccctcttcgg cgccgccgat gtagtcgcag atgtcccgga
     5161 ggtcggtccc catcaccgcc ccgaggtcgg ccttgaccag catgctcatc tggacatctt
     5221 cgtcggggtt ccagccgtca tagggtttgt cggggtgggc atcctcccag atgccctcaa
     5281 agaccgggcc ctggtggtag tagcgggtga cgccgtcccc ctggacgggg agattcgctt
     5341 ccagccagtg gtagtcgacg gtcgtctcgt ttaagacggt tttcccgtcg cttgcgaact
     5401 ttgcgatgcg cacctcggtg gtgcccgccg ccagcaccgg cgccgccaga gcggcgacca
     5461 gcagcagcac catgagcgtg atggttacca gtcggtgttg tttcatgtgg ttcaactcgc
     5521 gttgttggat tgtctgtgag gtttcggtcc tacgttcgtg cggggatccc gtccgggtgc
     5581 ggcgccccgg ggccgcacaa cctggagatc taaaaaaaga ggcatcagtc cttctttctg
     5641 agatacacaa agcctccgat tgtggcgccg atcaggatga ccgcgccgat accgatgagc
     5701 ggccagggcg gttctccgcc ggtcggcgtc ggggtgccgg gcgaaccggt ctgttgtgcc
     5761 tccgccgtcg tcgccgggac ggtccatgcc agggcataga tgctgaagtg ggcgacctcc
     5821 gcggtgatcg tccgcgcggc agggtccacg gtggcgggga tctcctgcca cttcccggtt
     5881 ccggggttat accagatcac cttcggtgtg gcgctgccga tctctgccca ctcctcagcg
     5941 gagagggtgt aggtgaggac gaccggcggg tcgaacgtgg cgcccgcagg gccgcagttg
     6001 agcgcgacgg caaccgttgt tcccggcggg gccgccggaa cttcggccgg agcgacctct
     6061 ctgcaggtca cctcgccgag cgggttgccg ccggcgtcaa gggcggtggt gccctccggt
     6121 atcgtcacgg tgccggtccc gccggcggtt ctgaccgcca cggtctcaag cacctccccg
     6181 gacgcagatg tggcaaggga ggcctgcccg gtctgcacct cgggagcggc ggtgggggcg
     6241 gccgattcgc gggcggtcac ggtattgccg gtgctcccgg tacccccggt gtttccggaa
     6301 ccacctgtgt tgtcggaacc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 cggaaccacc tgtgttgtcg gaaccgcctg tgttgtcggt gttgccgctg ccgccgctcc
     6481 ccgggatgct caggtcaagg accgtccctc cgccgattgc gaagaccgac gtctcctgtg
     6541 cggcgacacc gtcgacgtag aacgtgatcg tctcaccgct ctggtccgcg gttgccttca
     6601 cgagcaggcg gtcccccaca gaccggccag gattaccgta ttctccggtt tccgtcgtct
     6661 ggaacgagcc gcattccgtg cccccgatcg tcgccgtgat cacggttccg acgggtgcgg
     6721 gggagccccc gatcgtcacg ccaccggaga aaccgtgggg taacgtgggg gtgccctccc
     6781 cggccgccgc cgcaccggcg acgaggccga ggaggaggat tagtgtgatg ccctgtcgca
     6841 tcttcaccct gcaccaccct ctagccgctg atggcggcaa gcgtctccgc acgggtcgtg
     6901 tagatccagt agccctgcat gggctgcatc tcccgcgtct cgccggccgt ggtgcgtatg
     6961 atcgcgctct cgtaccgctg gtcgaccgcg tcgtaaccga tcagggtggc ccagctgttc
     7021 ccgagcgaca gcagggtggc cgccgcggat tcggggacgg tgtcggagaa cccgatcgcg
     7081 ttccagcctt taccgaggtc tttttggggc ggaagttcgg cggcgccgcc ggcaaaggtg
     7141 agcgggatct cggaggtgcc gttcgcgtag atccagaccg cgtcgagcgg ccggaaggtg
     7201 tcggtcaggc ccatctgcct ccagtcgtag tccagcccgt catagagcag gatcgagcgg
     7261 ccggcggtgt cgacgccgtc gaagatcgcg aaggtgttcg caccgtcggc gaggcgcttc
     7321 ggtgtcgaga cgaagttcca gccggggcgg aggacgatgg tatccgcctc ctccccgggt
     7381 tcggtcacga cgatggtctt cgtcgcgctg tcgcttccgg cggtgttcgc gaccatgagg
     7441 ctcacggtgt aggtgccggg ggcggtgtag gtatggaccg ggttctgttc ggcggaggtc
     7501 gcaccgtcgc cgaaggtcca ggaccaggag gtcggcgcgt tggtcgagcg atcggtgaag
     7561 gcgaccgcaa gcggcgcgtc gccggacgtg gtgctcgcat caaagccggc gaccggcggg
     7621 tttgccgtgc cgggctcgac gatgttgagc gtggtgatga tgacgtaccg ggcgccgctc
     7681 gggtcggagt tgacgacatc gtagtcgccg tagacggtgt agatggtggt gccgtttgca
     7741 agcgggacgt ccgggtcgta cttcgttgcg gcagaggtca tgccgtcgaa ggatcgccag
     7801 gcaaggttcg ccgtcgcgtc ctggacgttc gggtagtaga ccccgtcggg ggtggtgatg
     7861 tccgtcagcg agtaggtcgg gatcggtttc gtactcttcc agaggttgaa gatgaagccg
     7921 cttgcgttca gcatgccgcc gacgctctcc gccgggatct cgacgaagtc ctcgccgggg
     7981 acgtagcccc cggacctcgg cgagatggcg gcctggatcg tgctgccggc cgggatatcg
     8041 accgtgccct ggtacaggac gatgtaggga aggtccgagt caaggatggt gatcccggtc
     8101 gatgcctcgg ccgagccggc atcgtttgcc acggtcaggg tgatggtgta gttgcccgca
     8161 gcagggtacg cgtatgaagt gtcctgcccg acgacggtat cgccgttgcc gagatcccag
     8221 gtgtaggtga ggttgtcgcc ggtcgcggtc gccgcaaggc gaaccgaggc gccgacggca
     8281 taaccgctgt tcggcaacgg ggatatgatc gtcacgccgg ccggggtgcc atcctcaccg
     8341 ggccgggtcg gcaggagggt gacgttgagt tcgacgacgt aggtcgagtt ctcgaccgac
     8401 gggagttccc accagtcgaa ctggctcatg tcgccgtacc agatgtagta ggtgccggat
     8461 ttgctgatgt agccgtttaa ggctttcttg aatttgacgt cgttaccggt cccgagggtc
     8521 tcgtagaact tccacgcgta gtcggggccg aagtcttccg tcggataggt gacgccgtcg
     8581 atggtgaggg tcaggagggg ctttctgtcg gtgtagcccg cgtcgctctc ggcgtaggtg
     8641 taggtgtagc cggcggcggc aaggatgttg aagaacgtgt tgccgtcgta ggtggtgccg
     8701 tcgacgacga tcggctcgtc cgcgtagacc gtgacagcgg tgttgaagac gatcgggtca
     8761 ggtttggtga cggtgatctc ggccagtttg gaggactcgc cgtagctgtt gccgaccgtg
     8821 aggttgacgg tgtaggtacc ggccgcaggg tagacgtgga ccgggctctg ctcggtgctg
     8881 tcggcagtgc cgtcgttctc gaagtcccag gcccaggtgg tcggggctcc ggtcgaagcg
     8941 tcggtgaacg ccaccgcaag cggcagctcg ccgctcgtta cgttcgccgt gaagtcagcc
     9001 accggggcgc ccggttcagc cggccgggtc ggcaggaggg tgacgttgag ttcgaggacg
     9061 tagatcgagt tctcgacggt cgggtcgtcc agggagccga actggctcgt atcgccgaac
     9121 cagatgtagt aggtccccga gttgtcgatg tagccgttca gggctttctt gtacttgtag
     9181 gcgctcgaac cgaggccgtc gtagaacttc cacttgtagt cggggccgaa gtcctccgtc
     9241 gggtaggtga cgccgtcgat ggtgagggtc aggagcggct ttctgtcgcc gtaacccgcg
     9301 tcgctctcgg cgtaggtgta ggtgaacccc gccgcggcga ggatgttgaa gaacgagttg
     9361 ccgccgtagg tggtgccgtt gacgacgatc ggctcgtccg cgtagaccgt gacgg
//
LOCUS       DAOSII010000075         9266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_7101_length_9266_cov_50.957768, whole genome shotgun sequence.
ACCESSION   DAOSII010000075 DAOSII010000000
VERSION     DAOSII010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9266
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7101_length_9266_cov_50.957768"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            527..1327
                     /locus_tag="PLV90_06745"
     CDS             527..1327
                     /locus_tag="PLV90_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOF96852.1"
                     /translation="MIIESVNLVYFSPTGTTRAVVRGIARGLDPGNVEVRDITLPDAR
                     IEPLQVSENDLLVIGVPVYRGRVPALLAGWLHALSARNTPAVCVVVYGNRIYDDALIE
                     LGDILTQRGCRPIAGAVYIGEHSLSTDDVPIAPGRPDAGDLFHAESFGRKIQEKLDAI
                     PSADRITDIALPGCRPYRGDSRLQAADFIAVGDACIQCGICAEVCPVGAIDPEESRRI
                     DTAACITCCACIKNCPQHARSIKPGSVHDMSVRLHALCGERKEPECFL"
     gene            1353..2189
                     /gene="nudC"
                     /locus_tag="PLV90_06750"
     CDS             1353..2189
                     /gene="nudC"
                     /locus_tag="PLV90_06750"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HOF96853.1"
                     /translation="MEHHPFYAVRPLLPVYPEDPSPHERRWVLVRDSSVFFRNDPAAG
                     TVLMPDPLPEGLSCGEPVYLGTRDDIAYYAAGVAAGAEPPAGWQPAPVRELSGKVPEG
                     DMAIASYAVRILDFDRSTAFCGRCGARTRPLTTERARACTACGRIVYPRISPAIIVLI
                     KKGEEILLARSPRFTPGVFSVIAGFNEPGENLEQTVHREVGEEVGITVKNLCYFGSEP
                     WPFPDSLMIGFVAEHAGGEIRIDGREIEAAGWFTRDALPSLPSGASISRALIEAWRRR
                     EI"
     gene            2281..2583
                     /locus_tag="PLV90_06755"
     CDS             2281..2583
                     /locus_tag="PLV90_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YunC family protein"
                     /protein_id="HOF96854.1"
                     /translation="MQHETVRLTGKDADGYVIPLGPANLVAVVTDTGMVGCGAFDVDA
                     LEKFGYPAARVKPAGGASSIATVEDLLRGEIKGANRYAQERGVAAGMTGREALDRL"
     gene            2678..3196
                     /locus_tag="PLV90_06760"
     CDS             2678..3196
                     /locus_tag="PLV90_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96855.1"
                     /translation="MEGGAISTETATVAAIGLAAVLTIAVAAVLLIPGVSGPARPFSA
                     PLPAEIVDGYGGAPVIRMAGTGVDLAACRIYLVDPAGALHEVQTAILTNTTLGEGMAA
                     YIFYLPLDDRPAASGYWITDEPGLVFTADYHVAVRPFSPGGQWRLVVYDRESMKNRVD
                     QVLPITGPASPA"
     gene            complement(3171..5165)
                     /gene="metG"
                     /locus_tag="PLV90_06765"
     CDS             complement(3171..5165)
                     /gene="metG"
                     /locus_tag="PLV90_06765"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOF96856.1"
                     /translation="MSGKPLLVTCGLPYTNGPCHIGHLRTYVPADFYVRYMRRSGEEV
                     VFVCGSDNHGTPIVISAEREGSTPRELSERYHEHFYETFRRMEVIFDRFGMTDDAMNR
                     DTTRSIVERLVDNGYVYSKTISQSYCPECERFLPDRYVEGVCPHCGAVARGDECDQGC
                     GQHLEPGEIKDAICKICGGKAEYREQEHYFFKLSAFRDFLLDYLPTLGGTATARNYAL
                     GWVKELLHDWCITRTMEWGVPFPGSDDLVVYVWVDAPIGYISFTKEWAESAGADWKDY
                     WCGDDTRVAHFIGGDIIYHHCIFWPALLKGAGYGLPSAVVASGMVTIEGKKFSKSRGY
                     VVWTNDDYLDQGLPADYLRYYLLSYTNHTKELDFSWKVYAERVNKEIVGTLGNFLYRT
                     MYFAEKEFAGVPDRAPQPAILEEIERSLAGIDAAMRDYDFKNAVDSMMALASFGNAYI
                     QNNAPWKLIKEDRAAAQQVIADCLQIVKALVLVFQPLMPDAMQRAWTMLGYGDDIAAH
                     PIAEATAPVGARALPKPSPLFAKIEEAQVNDLEKVLNKRVDAANAAAAKKTSTVSIEE
                     FGKLEIRVARVVSAEPIKGSKKLYKLVVDLGGEKRQVVSGIAQFYSPGELVGKDVAMI
                     VNLAPATIFGVESRGMILAAGDEASLLVPLRPVRPGTPVR"
     gene            5208..5996
                     /locus_tag="PLV90_06770"
     CDS             5208..5996
                     /locus_tag="PLV90_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96857.1"
                     /translation="MARRITIPVRSFGSEVGTPAIPEVAEWLKGMRGEEADLATYRLS
                     RSLADQESVAVPAAGGMFYGERLSGAFTGMVDGVLVDEPGIDPSAPAADARYVVARRR
                     DAWFALPAPHALGLRDAYIDDEEEFAGVIVAGYARLAREMRDQGVRGHVLVADQADEA
                     ELERLAGNRFLFFPRDPGRFDLEVLLEYQDDLILPAGDLDRAADLMERFRVRKLILLD
                     AGVDDLLAATALVDPDMLEVGGYCSGEDCPDYWKTLVDRAFIAR"
     gene            complement(5983..6957)
                     /locus_tag="PLV90_06775"
     CDS             complement(5983..6957)
                     /locus_tag="PLV90_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HOF96858.1"
                     /translation="MSRSDQKSGTRGTNLMQALSERTATVVHLTHNDLDAVGSDAIHR
                     RKYGDVFTVWSSVGKFLANFDAVAGSPGRGDLLSISDIGYQPGVEQRLAKARSNGWRI
                     EWRDHHRWKDEEVRAVEKKTSLLRIDTGTCATGIVARDLAGDDAVATEIARVVCDYDL
                     WKHQDPRSKVLGQVVMRKGCREYVRDNLVRGIIVDEKVEDEYAGIVREMERDIKKSLR
                     HTTIIEGERYRIAFTPLYGYPSETAHTIRDELDTDIEVVVSSNGRISIRSVPPISHLI
                     AREFSGGGHPHAAGGSFPFNLLDRILFWLIKRNRHYRRLAGVAESIAR"
     gene            7084..7157
                     /locus_tag="PLV90_06780"
     tRNA            7084..7157
                     /locus_tag="PLV90_06780"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7118..7120,aa:Phe,seq:gaa)
     gene            7500..8054
                     /locus_tag="PLV90_06785"
     CDS             7500..8054
                     /locus_tag="PLV90_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96859.1"
                     /translation="MQSVFHPTEVFFSPYTKAEIREILADRVRQGLYPGVMPAPLLDR
                     IAGIAADEQDIRVGLDLIGMAVMQAEADGRRRVDAGDVAAAARTVIAPLLRTRIAGLS
                     PGERTLLCRIAEQSRERADMTSGAVFEATREYLPIGKSTYHEHLDRLVKTGIVDLVPG
                     QGRGREIRLRYDPDTVAGICRLPD"
     gene            8296..8568
                     /locus_tag="PLV90_06790"
     CDS             8296..8568
                     /locus_tag="PLV90_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96860.1"
                     /translation="MQHDTRPPDGEGTEPKNSGGSPDPECDRTVGQTATGTSPSEEIN
                     VSPVPLFLVPQAVAGVIRRHGRAVREIHVRRTRNHQYTIDVEQGRA"
     gene            8583..8897
                     /locus_tag="PLV90_06795"
     CDS             8583..8897
                     /locus_tag="PLV90_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96861.1"
                     /translation="MISCTAAGGLIVHVEAEQYRIAPEDAASLIFSGRPAPVTRSRGR
                     RAGSTITGEVAIEGYAAVHPAGRAVVIRTREGAWIIPLVSFRRVARGEAASAPLFLGV
                     TV"
     gene            8894..>9266
                     /locus_tag="PLV90_06800"
     CDS             8894..>9266
                     /locus_tag="PLV90_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96862.1"
                     /translation="MSSEILSLVRMLADGVVEVRALADGVTHSGYFDDYESLARAAEA
                     LDADPSVAGIYVTLNAVNPALLARRANRIKLRLSRKDATTADADITRRRWLPVDIDPV
                     RPSGVSSTDAEHEAAIAAADRI"
BASE COUNT         1557 a         3011 c         3050 g         1648 t
ORIGIN      
        1 catagcgccg ggcttctaac ccggatgtcg ggggttcgaa tccctccagg cccgtctgtt
       61 cttgatatcc acgagaggag tgcatctcct ggcgaatcac ggctgttttt ttgtgatcag
      121 cgcgtcgatg gaccgggaga gattgcacgg ttcttacacg agggcctccc cggggcccgg
      181 ccggatctcc tctaacccgc tcgcctcgcc cccggcgcgg ggccgcgccg tatcggggta
      241 cgggtatctc cgccgtctta cgagaggcct gaagggcgcc cggcatcttc tccgggggaa
      301 ggttcgtccg gcatggtgcc cggccgccgt acgtgcatcc aactgcaggt tattcaacaa
      361 tcaacggcta cagtacaacc agcagtgaga catctccggg aaccataccg tccgatgaag
      421 ggtggatgat tccggacccc gagatccctt tctgcaaggc gaccggggat gctgtccggg
      481 gtgctctcgg agatgcatgg agacctcaat tgatggagag aacactatga tcatagaatc
      541 ggtgaacctg gtctacttct cgcccaccgg gacgacgagg gcggttgtcc ggggtattgc
      601 ccgcgggctg gaccccggca acgttgaggt gcgggacatc acgctgccgg atgcaaggat
      661 agaaccgctc caggtctcgg agaacgatct gctcgtcatc ggcgtgccgg tgtacagggg
      721 gcgggtgccg gcgctcctgg ccggatggct gcatgcgctc tctgcccgga ataccccggc
      781 agtctgtgtc gtcgtctacg gcaaccgcat atacgacgat gcgctcatcg agcttggcga
      841 tatcctgaca cagcgcgggt gcaggccaat tgccggtgcg gtctacatcg gggagcactc
      901 gctctccacc gatgacgtgc cgatagcccc gggccgcccg gatgcaggcg acctcttcca
      961 tgcggagtcg ttcgggcgga agatacagga gaagctcgac gccatcccct cggccgaccg
     1021 gatcaccgat atcgccctgc ccggctgccg cccttaccgg ggagattcga ggctgcaggc
     1081 tgctgacttc atcgcggtcg gcgatgcgtg catccagtgc ggcatctgtg cggaggtatg
     1141 cccggtcggc gccatcgatc cggaggagag ccgccggatc gatacggcgg cgtgcatcac
     1201 ctgctgcgca tgcatcaaaa actgcccgca acatgcccgg tcgatcaaac ccgggtcggt
     1261 gcacgatatg tcggtgcgcc tccacgcact gtgcggcgag cgaaaggagc cggagtgctt
     1321 cctgtaaggg agagaacctg aggatacaca atatggagca ccacccgttc tacgcagtcc
     1381 gccccctcct ccccgtctac ccggaggatc cgtcgccgca cgagaggcgc tgggtcctgg
     1441 tccgggacag ttccgtcttc ttcaggaacg atcccgcggc cggcaccgtt ctgatgccgg
     1501 acccgctccc ggaaggtctc tcgtgcggcg agccggtcta cctcgggacc cgcgacgata
     1561 tcgcctacta cgctgccggg gtcgctgccg gagccgagcc gccggccggc tggcagccgg
     1621 cgcccgtccg ggaactctcc gggaaggttc cggagggcga catggcgatc gcctcctacg
     1681 ccgtgcggat cctcgacttc gaccggtcga ccgccttctg cggccggtgc ggcgcacgaa
     1741 cccgcccgct cacgaccgag cgggcacggg cctgcacggc ctgcggccgc atcgtctacc
     1801 cccggatatc ccccgccatc atcgtcctga tcaagaaggg cgaagagatc ctcctcgccc
     1861 gctccccccg cttcacgccg ggggttttct cggtcatcgc cggcttcaac gagcccggtg
     1921 agaacctcga acagaccgtc caccgcgagg tcggcgagga ggtcggcatc acggtgaaga
     1981 acctgtgcta cttcgggagc gaaccctggc cgttccccga ctcgctcatg atcggcttcg
     2041 tcgcggagca tgccggggga gagatccgga tcgacggccg ggagatcgag gccgccggct
     2101 ggtttacccg cgatgccctg ccttccctcc cctcgggggc gagcatatcg cgggcgctca
     2161 tcgaagcctg gcgccggcgg gagatctgat ccgctccatg cagcgccccg gtgcgatacg
     2221 ggaagagcac ggaagggggg aatcgcaata ttgatgacgc cagatggcca accacgccct
     2281 atgcagcatg agacggtcag gctgaccgga aaggacgccg acggctacgt catacccctc
     2341 ggcccggcaa acctcgtggc ggtggtgacc gatacgggca tggtcgggtg cggcgcgttc
     2401 gacgtcgatg cgctcgagaa gttcggctac cccgcggcgc gggtcaaacc cgcgggaggc
     2461 gcatcctcga tcgcgaccgt cgaggacctc ctccgcggcg agatcaaggg ggcgaaccgg
     2521 tatgcgcagg agcgcggcgt tgccgccggc atgaccggga gagaggcgct cgaccggctt
     2581 taggccttta tcggttcccc cttatttccg gagcatatct cctcgctcac cgctccaccc
     2641 ataatttcaa ataccgggcg gcaccctaag ggtgctcatg gagggggggg ccatatccac
     2701 ggagacggcg acggttgcgg ccatcgggct tgccgcggtg ctgaccatcg ccgtagccgc
     2761 ggtcctcctc atacccggag tctcaggccc tgcccggccc ttctccgccc ctctcccggc
     2821 ggagatcgtc gacggctacg gcggggcgcc ggtgatacgc atggccggga ccggggtcga
     2881 ccttgcggcc tgccggatct acctcgtcga cccggccggt gccctgcacg aggtgcagac
     2941 cgctatcctc acgaacacga cgctcggcga aggcatggcg gcgtatatct tctatctccc
     3001 cctggacgac cggccggcgg catcgggcta ctggatcacc gacgagcccg gcctggtctt
     3061 caccgccgac taccacgtcg ctgtccgccc gttctccccg ggcgggcagt ggcggctcgt
     3121 cgtttacgat agggaatcga tgaagaacag ggtcgatcag gtcctcccga tcaccggacc
     3181 ggcgtccccg gcctgaccgg ccggaggggg accaggagag aggcctcgtc gccggcggcg
     3241 aggatcattc cccggctctc gacgccgaag atcgttgccg gcgccaggtt cacgatcatc
     3301 gcgacgtctt tgcccaccag ttcgccgggg gagtagaact gagcgatgcc gctgaccacc
     3361 tggcgttttt cgcccccgag gtcgacgacg agtttgtaga gcttcttcga gcccttgatc
     3421 ggttcggccg agacgaccct tgccacccgg atctcgagtt tcccgaactc ctcgatggag
     3481 acggtgctcg tcttcttcgc ggccgccgcg ttcgccgcat ccacccgctt gttgaggacc
     3541 ttctcgaggt cgttcacctg cgcctcctcg atctttgcaa agagggggga gggttttgga
     3601 agcgcccgcg ccccgaccgg cgccgtggcc tccgcgatcg ggtgggcggc gatatcgtcg
     3661 ccgtagccga gcatcgtcca ggcccgctgc atcgcgtccg gcatcagggg ctggaacacc
     3721 aggacgagcg ctttcacgat ctggaggcag tcggcgatca cctgctgtgc cgccgcccgg
     3781 tcctccttga tcagtttcca gggggcgttg ttctggatgt aggcgttccc gaacgacgcc
     3841 aaagccatca tcgagtcgac ggcgttcttg aagtcgtagt cccgcatcgc cgcatcgatg
     3901 ccggcaaggg agcgctcgat ctcttcgagt atggccggtt gcggggcacg gtcgggcacg
     3961 ccggcgaact ccttctccgc gaagtacatc gtccggtaga ggaagttccc gagcgtgccc
     4021 acgatctcct tgttcacccg ctcggcgtag accttccagg aaaagtcgag ctccttggtg
     4081 tggttcgtgt aggagaggag gtagtagcgg aggtagtcgg ccgggaggcc ctgatcgaga
     4141 taatcatcgt ttgtccagac gacgtagccg cgggacttcg agaacttctt cccctcgatc
     4201 gtgaccatgc cgcttgcgac cacggcgctc gggagaccgt agcccgcccc tttgaggagg
     4261 gccggccaga atatgcagtg gtggtagatg atatccccgc cgatgaagtg ggcgacgcgg
     4321 gtatcatcgc cgcaccagta gtccttccag tcggctcccg cggactctgc ccactccttc
     4381 gtgaacgata tatacccgat cggcgcgtcg acccagacgt agacgacgag gtcgtcgctc
     4441 cccgggaagg gaacgcccca ctccatcgtc cgggtgatgc accagtcgtg caggagctcc
     4501 ttcacccagc cgagggcgta gttccgggcg gtcgcggtgc cgccgagggt cgggaggtag
     4561 tcgaggagga agtcgcggaa cgccgagagc ttgaagaagt agtgctcctg ctcgcggtac
     4621 tccgccttcc cgccgcagat cttacagatc gcgtccttga tctcgccggg ctcgaggtgc
     4681 tgcccgcatc cctggtcgca ctcgtcgccc cgggcgacgg cgccgcagtg ggggcagacc
     4741 ccctcgacgt agcggtcggg aagaaaccgc tcgcactccg ggcagtagct ctggctgatg
     4801 gtcttagagt agacgtagcc gttatcgacg aggcgctcga cgatcgagcg ggtggtgtcg
     4861 cggttcatgg cgtcgtcggt catgccgaac cggtcgaata tgacctccat ccgccggaac
     4921 gtctcgtaga agtgctcgtg gtaccgttcc gagagttcgc ggggggtcga gccctcccgc
     4981 tcggcactga tcacgatagg ggttccgtgg ttgtcggaac cgcatacaaa gacgacctcc
     5041 tccccggacc ggcgcatgta ccggacgtaa aagtccgccg gcacgtaggt ccgcaggtgc
     5101 ccgatatggc agggaccgtt cgtgtacggg agtccgcacg ttaccagcag cggcttacca
     5161 ctcatcctta tctactctgg ttgcgatgct aataaagaca tcagcgcatg gcacgacgga
     5221 ttacgatacc ggtgagatcg ttcggctccg aggtcggaac gcccgcgatc ccggaggttg
     5281 cggagtggct gaaggggatg cggggagagg aagcggacct cgcgacctac cggctcagcc
     5341 gctcgcttgc cgaccaggag agcgtggcgg tgcccgcggc gggcggtatg ttttacggcg
     5401 agcggttgag cggggccttc acggggatgg tggacggggt gctcgtcgac gaaccgggga
     5461 tcgatccctc cgccccggcc gcggatgccc gttacgtcgt ggcgaggcgg agagatgcgt
     5521 ggtttgccct ccccgcccct cacgcgctcg ggcttcgcga cgcctacatc gatgacgagg
     5581 aggagttcgc cggggtgatc gttgccggat atgcccgtct cgcgcgggag atgcgcgatc
     5641 agggcgtgcg ggggcacgtc ctcgtggccg atcaagcgga cgaggccgag ctcgagcggc
     5701 tggccggcaa caggttcctc ttcttcccga gggatccggg gaggttcgac cttgaggtcc
     5761 tgctcgagta ccaggacgac ctcatcctcc cggcaggcga cctcgaccgg gcggcggacc
     5821 tgatggagcg gttccgcgtc cggaagctga tcctccttga tgcgggggtg gatgaccttt
     5881 tagccgccac ggcgctcgtc gacccggata tgctcgaggt tggcggttat tgctccgggg
     5941 aggactgccc ggactactgg aagaccctcg tcgaccgggc gttcatcgcg cgatagactc
     6001 ggccacgccc gcaaggcggc ggtagtggcg gtttcgcttt atgagccaga agaggatgcg
     6061 gtcgagcagg ttgaacggga acgagcctcc cgccgcgtga gggtggccgc cgccggagaa
     6121 ctcccgggcg atgagatggc tgatcggggg gacggagcgg atggatatcc ggccgtttga
     6181 agagacgacc acctcgatat cggtatcgag ctcatcgcgg atggtgtgcg cggtctcgct
     6241 cgggtagccg tagagggggg tgaaggctat ccggtagcgc tccccttcga tgatggtggt
     6301 gtgccggagg ctcttcttga tatcgcgctc catctcgcgg acgatcccgg catactcatc
     6361 ttctactttc tcatcgacga tgatgccccg gacgaggttg tcgcggacgt actcccggca
     6421 gcccttccgc atcaccacct ggccgagcac cttcgaccgg ggatcctggt gtttccagag
     6481 gtcgtagtcg cagacgaccc gtgcgatctc tgtggctacc gcgtcgtcgc cggcaaggtc
     6541 gcgcgcgacg atgccggtcg cgcaggtgcc ggtgtcgatc cggaggaggc tcgtcttctt
     6601 ctcgacggcc cggacctctt cgtccttcca gcgatggtga tcgcgccact cgatccgcca
     6661 cccgttcgac cgggccttcg ccagccgctg ctcgacgccc ggctggtagc ctatgtccga
     6721 tatgctgagg aggtcgcccc ggcccgggga gccggccacg gcgtcgaagt tcgcgaggaa
     6781 cttgccgacc gaggaccaga cggtgaagac gtccccgtac ttccgccggt ggatggcgtc
     6841 gctcccgacg gcgtcgagat cgttgtgcgt gaggtgtacg acggtcgccg tcctctcaga
     6901 gagcgcctgc atgaggttcg tgccgcgggt acccgacttc tgatcgctcc ggctcattgg
     6961 tcatatatcg gcatgcgatg agataaacag gcggctccgg gcccggtcgg ccgggtgcaa
     7021 agagccggcg ttccccgagc acagaattta ttagggacga cggataacac tgtaaggcag
     7081 aaagcccctg tagctcagac tgggagagcg ccagactgaa gatctggttg tccccggttc
     7141 aaatccgggc gggggcatgt ttttaccttt gttccaattc ctgagcctgt acagcgcttt
     7201 gtttttttct cctataccgt gccgatctaa cctgcaatag gccacaccac ctgaccattt
     7261 ttcatacccc ggtcgtgtag cacctctgtc acggtatccc gtacgtgggc tcgtgacaaa
     7321 actcccggca ccgcaattac cgccgccggc gaccgggcgg cgatcgtcgc ccgcctcaag
     7381 gagaaggatg caaaccttct tgtctgccct gacgatgcaa actacctcat cgccgcaggg
     7441 acctacaacc tcctcctcta ccagctcctc cggctctaag aggggtgggc cggcgtccgg
     7501 tgcagtcggt cttccatccc accgaggtct tcttctcccc ctacacgaag gccgagatcc
     7561 gggagatcct cgccgaccgc gtcaggcagg ggctctaccc cggggtgatg ccggcgcccc
     7621 tcctcgaccg catcgccggc atcgccgccg atgaacagga catccgggtc gggctcgacc
     7681 tgatcgggat ggcggtcatg caggcggagg cggatggccg ccggcgggtc gacgccgggg
     7741 atgtggcggc cgcagcccgg acggtcatcg cgccgttact ccggacccgg atagcagggc
     7801 tctcaccggg ggaacgcaca cttctctgcc ggatcgcgga gcagtcccgg gagagggccg
     7861 atatgacttc cggggcggtc tttgaggcga cccgggagta cctcccgatt ggaaagtcga
     7921 cctaccacga gcacctggac cgccttgtaa agaccgggat cgtcgacctc gtgcccgggc
     7981 agggccgggg gcgggagatc cggctccgct acgaccccga cacggtggcc gggatctgca
     8041 gattgccgga ctgaacttac agatcgccgg actgaaaccc cggatctccg gactgagcct
     8101 gagtttgcga tctcttgata tcagccgggg attgaacatt ccggcatttt tttcaggatt
     8161 tgagatcctc cgataaagta acgcttatat agggcttgtt tgaaattccg ggaccattgg
     8221 ctcacccttt taagcaacgg cgccagagaa tgatgtatgc gccggagatg agtcccggtg
     8281 caggagagaa caaaaatgca gcacgacacc agacccccgg atggggaagg aaccgaaccg
     8341 aagaacagcg gcggcagccc ggacccagag tgtgaccgca ccgtcggcca gaccgccaca
     8401 ggtacatcgc catcagaaga gataaacgtc tcccccgtcc cgctcttttt ggtaccgcag
     8461 gccgtggccg gcgttatccg gcggcacggc cgggcggtgc gggagatcca tgtcaggaga
     8521 accaggaacc accagtatac catcgacgtc gaacaggggc gggcctgatg ccggaggcgg
     8581 gtatgatctc ctgcacggcg gccggcgggc tcatcgtcca cgtcgaggcc gagcagtacc
     8641 ggattgcccc tgaagacgcg gcaagcctga tcttttcggg ccggccggcc ccggtgacac
     8701 gctcgcgggg gcggcgggca ggcagcacca tcaccggcga ggtggcgatc gagggctacg
     8761 cagcggtgca cccggcaggc cgcgcggtgg tgatccggac ccgggaaggt gcctggatca
     8821 taccgctggt atccttccgg cgcgtcgccc gcggggaggc ggcaagcgcc cccctctttt
     8881 tgggggtgac ggtatgagca gcgagatcct ctctctggtg aggatgcttg ccgacggcgt
     8941 ggtcgaggtc agggcgctcg ccgacggcgt cacccactcc ggctacttcg atgattacga
     9001 gagcctcgcc cgggccgcgg aagccctcga cgccgacccg tcggtcgccg ggatctacgt
     9061 caccttaaac gccgtgaacc ccgccctcct cgcccggcgg gcaaaccgga tcaagctgcg
     9121 cctctcgcgg aaagacgcca cgaccgcgga cgccgacatc acccgccgcc gctggctccc
     9181 ggtcgacatc gaccccgttc ggccgagcgg ggtctcgtcg accgacgccg aacacgaagc
     9241 ggctatcgcc gccgcggacc ggatag
//
LOCUS       DAOSII010000076         9121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_7216_length_9121_cov_47.598279, whole genome shotgun sequence.
ACCESSION   DAOSII010000076 DAOSII010000000
VERSION     DAOSII010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9121
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7216_length_9121_cov_47.598279"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="PLV90_06805"
     CDS             <1..1013
                     /locus_tag="PLV90_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOF96863.1"
                     /translation="ILGVLLMAPPVIMSAGGLDHINTVLAGVDPNFVEPWFPAAYAPY
                     AYATPEFLVSFGILLMVGLACAPHVINNVLAAKEARYFKWAPLVAFAIYAVVMFLVKF
                     TGFAVRSLATEGAITLPAAVNAQDYSFIVGVEYAMPSVLLWAFFAVIVLAAVMSTTDR
                     LMLTVGTSFAWDIYKNIFRPSASDDEVLLVSKVAVVLGAGGTLLLAINPPEMLAWLIW
                     MGIGVMLATFAVPLLAGLYWRGATKEGAIASMAVGLIAAGVFGYWSKFVGSLPVHFSF
                     YALLLSALAMIAVSLLTARISTDTLDTTRTGWFIQSPGQPSRAGSKGDAWLVEQDTPR
                     QH"
     gene            1205..1695
                     /locus_tag="PLV90_06810"
                     /pseudo
     CDS             1205..1695
                     /locus_tag="PLV90_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328087.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters.;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
     gene            1938..3326
                     /locus_tag="PLV90_06815"
     CDS             1938..3326
                     /locus_tag="PLV90_06815"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOF96864.1"
                     /translation="MQISVISGSPKGEKSVTFQYVRFLEQAFPDHTFTVVHAGRDIRA
                     IEEREEVWRGLLATVEGCEGVLWATPVYVMLVPAQLKRFIELVGERNAAEAFSGKYAA
                     CLTTSIRFFDHTAHAYLHGVCDDLGMLYTGFCSAHMEDLRNEAFQEQFILFFSDFLEA
                     IIEQRPVQRVFPPLPAASACRVLKTSPAAVDTRGRRVVILHDAEPGSGQEAMVRHLAA
                     CYAGPVQVAHVRDAGMKGGCLGCCRCAFENTCIYDDGFRAFWNEYVVPADILVMAGTV
                     RDRFFSAAWKQFFDRSFSNGHVPAFEGKQIAYLVEGPLGHLPNLREVLTGLATMEGAN
                     LAGIVTDESDRIDAVLHTLAERSVRLSERGYIAPATFPAVAGHKIFRDEIWGGMRAIF
                     KADDRYYRQHGLYDFPTRDYPKRIVTRATSLAMSIPAFRRDVVEKMPAYMVRPLEKAI
                     ETSTVLAKRRAK"
     gene            3497..4810
                     /locus_tag="PLV90_06820"
     CDS             3497..4810
                     /locus_tag="PLV90_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF96865.1"
                     /translation="MKTAVLGGGLTGVTLARLLHERGDDVTVLERGETIGGLCRSKTE
                     AGFTFDIGGSHIIFSRDAEALSFMLSALGGNADRRKRNTKILYKGRYVKYPFENGLMD
                     LPAEDRFFCINEFVRNLIAVEKGEVPPPANFAEWIVSTFGRGIAECYMLPYNEKIWNY
                     PADRMSHHWVDGRIPRPPIEDIIRSAIGIETEGYTHQAVFSYPVEGGIEALVRAIAEP
                     VLPAVRTGFTVSSIREEGDGFAVSDGRETVHAGRLISTIPLQNLLPCLSGVPADVRAA
                     CDALRYNSLCSVFIGIAGEVPDISWLYVPDEAAGLFNRVSFPSNYSTRVAPAGHSSIL
                     AEITYNDGDAVSRMADADLIEHTVTSLVAAGLIPSRDTVVYTGIARQEFAYVVYDIDY
                     QKNIGIVREFCREKGIDLVGRFSRFEYLNMDGCIRSAIDFLRGDG"
     gene            4807..5784
                     /locus_tag="PLV90_06825"
     CDS             4807..5784
                     /locus_tag="PLV90_06825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOF96866.1"
                     /translation="MKVNIFVEDFRFLRYIGCATAARTLYNHLARLPDMEVSRNLYHG
                     DFDLTHYHTFGPWAMYHLKFTEGVKVLTAHSTPRLNEGNVAFSKRINTIYPKIYRQFD
                     HIITISEPCRRETEQLVPDVPKTLIPNGVDRAYFKRDKKKRKSFRDEFEIEPGQRVVL
                     SVGQQTPRKGIYDFFRLADQHPDMTWVWVGGFPYGTLSKDYAKIQMLKTHAHANVIFT
                     GIIDDISRAYSGADVFFMPSHAETFGLVIVEALSAGLPVIARDIFEFKEIFGDSVLFF
                     GDLDDARVLVNNDAALRRCASRARASTENYDITLIAQRHQQLYRELIEA"
     gene            5781..6479
                     /locus_tag="PLV90_06830"
     CDS             5781..6479
                     /locus_tag="PLV90_06830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOF96867.1"
                     /translation="MISVVVPTYNEEQNIERCLASLADQTVPRETYEIIVVDGNSRDR
                     TRELAEPLADKVFIQTSKRVGGARNDGAMAASGDIIATTDADCILPRDWVERIGQNFA
                     ERDIVQLYGTVYPIEGGLKNHLSLLGANTFARLGCYTRTIYFTLGCNTAFDREAFIRA
                     GMYRCIDAGDDLEIAQRMRKLGRVRLDTRLKVGFSMRRYQQFGTLKSVWEWLYIVLSG
                     GDAGGATYSQREYK"
     gene            6484..6903
                     /locus_tag="PLV90_06835"
     CDS             6484..6903
                     /locus_tag="PLV90_06835"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOF96868.1"
                     /translation="MERTPSIPEAYARVVQRSDACEFARLLGMAVTEIEGGRVRVAMP
                     AEGTKNVHGTTHGGAIFSLADHAFGIAANMEGIDQIAISARIRYFTVPAADTLEAVAR
                     RVSETERTSVYAVDVFSGDRLVASFEGVGFKIGTVPE"
     gene            complement(6914..7405)
                     /gene="msrA"
                     /locus_tag="PLV90_06840"
     CDS             complement(6914..7405)
                     /gene="msrA"
                     /locus_tag="PLV90_06840"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOF96869.1"
                     /translation="MAQEKKIERATFGAGCFWGIEETFREVRGVVDTAVGFMGGSTEN
                     PTYPDVCTGKTGHTEVVQVTYDPGEVSYRDLLNVFWNAHDPTTLNRQGPDIGTQYRSV
                     IFYHTTEQEAEARASRAAVEQSGRFRRHVVTAIEPAGTFWRAEEYHQQYFAKHGGGHC
                     RVM"
     gene            7496..8041
                     /locus_tag="PLV90_06845"
     CDS             7496..8041
                     /locus_tag="PLV90_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HOF96870.1"
                     /translation="MIFESDTVEMVARLMALSARTAPKARGIDVIRTLIVTGDERRVL
                     ARAMREYGERHNAGFFIRDAGNVEASDACLIIGSLLADAVGLDCGGCGYATCAEMLKA
                     QGNLRSTPFCGPNCVVRMADIGIAVGSAVKTASIHNVDNRVMYTVGVGALSLGWLEGC
                     GVAYGIPLRASGKDIFFDRTR"
     gene            8056..>9121
                     /locus_tag="PLV90_06850"
     CDS             8056..>9121
                     /locus_tag="PLV90_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HOF96871.1"
                     /translation="MPIMKIRGGDLDLVEYEFTSFSPGEHIRPCGLQKEYRLSPVAGT
                     VVVRAPARIHLTVLDMNRFSPDHPGGGGIGFAIGVYCTAEVECTASGVDIDYTREPIL
                     RHFVEAFRQVVGYTGGFTIRARDHQYEHVGLGSTSTILIAVANALNAAVGSPLTPDEL
                     RLLLGNNFVEETAAGNVAFGFETGVGPAASTHGGMVVMGDGLALVYRHPFAGGKKVYI
                     AIPSSDISSAGEKEFDLLMNKARTLDYRDRALKSYLVLMDLIPALERGDLGAIGDVIW
                     EIEFRGSKRAEVEHHGFEIYRYMAALREAGLEFVGMSSVGPSIAVITGRPEEEVAAIL
                     EKAGLRIAIATAVDNEGLKVH"
BASE COUNT         1615 a         2938 c         2878 g         1690 t
ORIGIN      
        1 tgatcctcgg cgtcctcctg atggcgccgc cggtgatcat gagcgccggc gggctcgatc
       61 acatcaacac ggtccttgcc ggcgtcgacc cgaactttgt cgaaccctgg ttcccggcgg
      121 cgtatgcccc gtacgcctac gcgacgccgg agttcctggt ctcgtttggg atcctcctga
      181 tggtcgggct cgcctgcgca ccgcacgtca tcaacaacgt ccttgccgca aaggaggctc
      241 gttacttcaa gtgggcgccg ctcgtcgcgt ttgcgatcta cgcggtcgtt atgttcctcg
      301 tcaagttcac cgggtttgcc gtccggtcgc tcgccacgga gggggcgatc acgctccccg
      361 ccgccgtgaa cgcgcaggac tactccttca tcgtcggcgt cgagtacgcg atgccgagcg
      421 tgctgctctg ggcgttcttt gcggtgatcg tcctcgcggc ggtgatgtcc acgaccgatc
      481 ggctgatgct caccgtcggc acctcgtttg cgtgggatat ctacaagaac atcttccggc
      541 cctcggcatc cgacgatgag gtcctcctgg tatcaaaggt cgcagtcgtc ctcggggccg
      601 gcggcacgct cctccttgcc atcaacccgc ccgagatgct cgcgtggctg atctggatgg
      661 gtatcggcgt gatgctcgcg acgtttgcgg tcccgctgct cgccgggctc tactggcgcg
      721 gcgcgacaaa ggagggggcg atcgcgagta tggcagtcgg gctcatcgcg gccggggtct
      781 ttggctactg gtccaagttc gtaggatcgc tccccgtaca cttcagcttc tacgcgctcc
      841 tgctctcggc ccttgccatg atcgccgtga gcctcctcac tgcccggatc tctaccgata
      901 ccctcgacac cacccgcacc ggctggttca tccagtcgcc cggtcagcct tcacgggctg
      961 gcagcaaagg ggatgcctgg ctggtcgaac aggatacgcc ccgccagcac tgaggccggg
     1021 gcgctcccgg caccttctct ttttcttctg catgagagtg atggtgcacc gtgccgatcg
     1081 ccttcgcaaa gtccatattc ctccggggaa ccacatagac tgctgacata gccggcggac
     1141 agcgagatcg ggcgtgcagg agccggcatg ccggccgggt cgagccgggt cgtttgaggt
     1201 tgccatgagg atcgtcatct actacttcac cgggacaggc aactcgcttg ccgccgcaaa
     1261 aaagatcgcc gcagtcctcg ggggctgcga gatcgtccct atcgcatcgc tcgcaaacac
     1321 cccggggcca tcgctccgga ggccggccgg atcgggatcg tctgcccggt ctacttctcc
     1381 gggctcccgg cgatggtgac ggcgtttgca aagcgcctcg ataccgcacc ggcgggctac
     1441 ctcttcgcgg tcgtcacgca cgggggaggc ggaggcgccg ccgtctgccc ggcgggcaac
     1501 atcgatatgg tcgatgggcg accagtctgg aaccaccgct gcgaactctg ctgcgggtgc
     1561 atccacctct gcccggccgg ggctatccag gccgggagcc gaaccgaggg gaggcagcgc
     1621 taccggcacc cggacgtgac ggttgcggat atggagaggc aggcggggcg aaaccgcacg
     1681 agaagagccg ggtgacaggt cccacgcagc gttcgtaaga ggcaattatg ccgtattaca
     1741 tcgatgccgg aagaataagc cttgacgacc tgcaaaagag gatcacggag accgatccgg
     1801 ttccaggccg tggcggcacg gtcctatgct ttccgcgtga tgtcgttttg cgccggggag
     1861 gtgcgtatcc ccccttcccg caaggtatat cagcccgccg gcttcagatc cgtatataag
     1921 acacaagcgg ctgaaccatg cagatctccg tcatctccgg gagtcccaag ggcgagaaga
     1981 gcgtcacttt ccagtatgtc cggttcctcg agcaggcatt cccggaccac accttcactg
     2041 tcgtgcatgc gggaagggat atcagggcga ttgaagagcg ggaagaggta tggcgcgggc
     2101 tgctcgccac ggtggagggg tgcgagggcg tgctctgggc aacgccggtc tacgtcatgc
     2161 tggtcccggc ccagctcaag cgtttcatcg aactggtcgg cgagcggaac gcggcggagg
     2221 cattctccgg gaagtatgcc gcctgcctca ccacctccat ccgcttcttc gatcacaccg
     2281 cccacgccta cctgcacggg gtctgcgacg acctcgggat gctctacacc ggtttctgct
     2341 ccgcccatat ggaagacctc cgaaatgagg cgttccagga acagttcatc ctcttcttct
     2401 ccgatttcct ggaggcaatc atcgagcaac ggccggtcca gagagtattt ccaccattgc
     2461 ccgcagcctc cgcttgccgc gttctaaaaa cctcgccggc cgcggtggat acccgcggaa
     2521 gacgggtggt catcctccac gacgccgaac ccggttccgg ccaggaggcg atggtccggc
     2581 accttgcggc gtgctacgcg ggtccggtcc aggtcgccca cgtcagggac gccgggatga
     2641 aaggcggttg cctggggtgc tgccggtgcg ccttcgagaa cacctgcatc tacgacgacg
     2701 gtttccgggc gttctggaac gagtatgtcg tcccggcgga tatactggtc atggccggga
     2761 ccgtccggga ccgattcttc tccgccgcct ggaagcagtt cttcgaccgg agtttctcaa
     2821 acggccacgt gccggcattc gagggaaagc agatcgcgta cctggtggag gggccgctcg
     2881 gccacctccc gaacctccgg gaggtcctca cgggcctcgc gacgatggag ggggccaacc
     2941 ttgcagggat cgtgaccgac gaatcggaca ggatcgatgc cgtcctgcac accctcgccg
     3001 aacgctccgt ccggctctcg gaacgggggt atatcgcgcc ggccaccttc ccggccgtcg
     3061 ccggccacaa gatcttccgt gacgagatct ggggcgggat gcgggcgatc ttcaaggcgg
     3121 acgaccggta ttaccggcag cacgggctct atgactttcc cacccgggat tacccgaagc
     3181 ggatcgtcac ccgggcgacc tctcttgcga tgagcattcc tgccttcagg cgggatgtgg
     3241 tagagaagat gcccgcctac atggtccggc ccctcgagaa agcgatcgaa accagcacgg
     3301 tcctcgcgaa gagaagggca aaataggagc attcctgatc gccggaagga cgcccgatcc
     3361 tctacgcatc accggacgcc tccgcggatc gccggaaggg tgccggggtt gcggcccgat
     3421 ttctcccctg gagaggtcaa ctcggtgcag cgcggatggt caaccatata aggcttcggc
     3481 attaaacaat aggaatgtga aaacggcggt actgggcggc ggtctcaccg gggtaacgct
     3541 ggcccgcctg ctccacgagc ggggcgacga tgtgaccgtt ctcgaacgtg gcgagacgat
     3601 cggggggctc tgccgctcga agacggaggc ggggttcacg ttcgatatcg gggggtcgca
     3661 catcatcttc tcccgcgacg cggaagccct ctccttcatg ctctcggccc tcggcgggaa
     3721 cgccgaccgg cggaagcgca acacaaagat cctctacaaa gggcgttacg tcaaataccc
     3781 cttcgagaac ggccttatgg atctgcccgc ggaggaccgt ttcttctgca tcaacgagtt
     3841 cgtgaggaac ctgatcgccg tcgagaaggg cgaggtcccg ccgccggcaa actttgccga
     3901 atggatcgtc tccacgttcg gccggggcat cgccgagtgc tacatgctcc cctataacga
     3961 gaagatctgg aactacccgg ccgaccggat gtcgcaccac tgggtggacg ggcggatccc
     4021 ccgcccgcca atcgaggata tcatccggtc ggccatcggt atcgagaccg agggctacac
     4081 ccaccaggcg gtcttctcct atccggtgga gggcggtatc gaggcgctgg tcagggcgat
     4141 cgcagaaccg gtcctccccg ccgtccggac cggctttacg gtcagttcca ttcgcgagga
     4201 gggcgatggg tttgccgtca gcgacggccg cgagaccgtc catgccggtc gcctgatatc
     4261 gacgatcccc ctccagaacc tgctcccctg cctttcgggt gtcccggccg acgtgcgggc
     4321 ggcctgcgac gccctccgct acaactcgct ctgctcggtc ttcatcggca tcgccggcga
     4381 agtccccgac atatcctggc tgtatgtccc cgacgaggcg gccggcctct tcaaccgggt
     4441 atcgttcccc tcaaactaca gcacgagggt cgcacccgcc ggccactcat cgatccttgc
     4501 cgagatcacc tacaacgacg gcgatgcggt ctcccgtatg gcggatgccg acctgatcga
     4561 gcacaccgtc acgtcgctcg tcgccgccgg gctcatcccc tcgcgggata ccgtcgtcta
     4621 caccggcatc gcgcggcagg agttcgccta cgtcgtctac gatatcgact accagaagaa
     4681 catcgggatc gtcagggagt tctgccggga gaaggggatc gacctcgtcg gccggttctc
     4741 ccggttcgag tacctcaaca tggacggctg catccggagc gcgatcgact ttttgagagg
     4801 agatggatga aggtcaacat attcgtcgaa gatttccgtt ttctgcgcta catcgggtgc
     4861 gcaacggccg cacgaaccct gtataaccac cttgcccggc tccccgatat ggaggtctcc
     4921 cgcaacctct accacggcga cttcgacctg acccactacc acactttcgg gccgtgggcg
     4981 atgtaccacc tgaagttcac cgagggcgtg aaggtcctga ccgcccactc gaccccacgg
     5041 ctgaacgagg ggaacgtcgc tttctccaag cggatcaaca cgatataccc gaagatctac
     5101 cggcagttcg accacatcat aacgatatcg gagccctgcc gccgcgagac cgaacagctc
     5161 gtccccgacg taccaaagac cctcataccg aacggcgtcg accgggcgta cttcaagcgg
     5221 gacaagaaga agaggaagtc gtttcgcgat gagttcgaga tcgaacccgg ccagagggtg
     5281 gtgctctccg tcggccagca gaccccccgg aaagggatct acgacttctt caggcttgcc
     5341 gaccagcacc ccgacatgac ctgggtctgg gtgggcgggt tcccctacgg gacgctctcg
     5401 aaggattacg cgaagatcca gatgctcaag acccatgccc acgcaaacgt catattcacc
     5461 gggatcatcg acgacatatc ccgggcgtac agcggggcgg acgtcttctt catgccgtcg
     5521 cacgccgaga cgttcggcct cgtgatcgtc gaggccctct cggcgggctt gccggtcatc
     5581 gcccgggata tctttgagtt taaggagatc ttcggcgact cggtcctctt ctttggagat
     5641 ctcgacgatg cgcgggttct cgtgaacaat gatgcggccc tgcggcgctg cgcctcccgt
     5701 gcccgcgcct cgacggagaa ctacgacatc acgcttatag cacaacggca tcaacaactg
     5761 taccgggagt tgatcgaggc atgatctcgg tcgtcgtgcc gacgtacaac gaagagcaga
     5821 acatagagcg ctgccttgcg tctcttgccg accagacggt cccgcgggag acctacgaga
     5881 tcatcgtcgt cgatgggaac tcaagggacc ggacccgcga gctcgcagag cccctcgcgg
     5941 ataaggtctt catccagacg agcaaacggg tggggggagc ccgaaacgac ggtgcgatgg
     6001 cggccagcgg cgacatcatc gcgacgaccg acgccgactg catcctcccc cgcgactggg
     6061 tggagcggat cgggcaaaac tttgctgagc gggatatcgt gcagctctac ggcacggtct
     6121 acccgatcga gggggggctt aagaaccatc tctcgcttct gggtgcaaac acctttgcgc
     6181 gtcttggctg ctacacccgg acaatctact tcacgctcgg gtgcaacacg gcgtttgacc
     6241 gcgaggcgtt catccgggcc gggatgtacc gctgcatcga tgccggggac gaccttgaga
     6301 tagcgcagcg gatgcgtaaa ctcggcagag tccgcctcga cacccgcctg aaagtcggat
     6361 tctcaatgcg gcgctaccag cagttcggga cgctgaagtc ggtttgggaa tggttataca
     6421 tcgttttatc cggcggcgac gccggcggcg ccacctactc gcagcgggag tataagtagc
     6481 tccatggaga ggaccccatc catcccggag gcctatgcga gggtggtgca gcgctccgat
     6541 gcctgtgagt ttgcccgcct gctcggtatg gcggtcacgg agatcgaggg cggccgtgtc
     6601 cgggtggcga tgccggcgga gggcacgaag aacgtccacg gcacgacgca cgggggtgcg
     6661 atattttctc ttgccgatca cgcgttcggc atcgccgcga atatggaggg aatcgaccag
     6721 atagcgatat ccgcccgtat ccgttacttc accgtcccgg cggcggatac gctcgaagcg
     6781 gtcgcccgca gggtctctga gaccgagagg acgtcggtgt acgcggtgga tgtcttctcg
     6841 ggcgaccgcc tggtcgcctc gtttgaggga gtagggttca agatcgggac cgttccggaa
     6901 taggaccggc aggtcacatg accctgcagt gcccgccgcc gtgctttgca aagtactgct
     6961 ggtggtactc ctcggcccgc cagaacgtcc ccgcgggctc gatcgcggtg acgacgtggc
     7021 gccggaacct ccccgattgt tctaccgccg ctctcgacgc ccgtgcctct gcctcctgct
     7081 ccgtcgtgtg gtaaaagata acggacctgt actgcgtccc gatgtcgggc ccctgccggt
     7141 tcagcgtcgt cgggtcgtgg gcgttccaga agacgttgag gaggtcgcga tacgagacct
     7201 ccccggggtc gtaggtcacc tgcacgacct cagtatgccc ggtctttccg gtgcagacat
     7261 cggggtaggt cgggttctcg gtgctgccgc ccatgaaccc caccgccgta tccacgacgc
     7321 cgcggacctc ccggaacgtc tcctcgatgc cccagaaaca ccccgcaccg aaggtggcgc
     7381 gttcgatctt cttctcctgt gccatgatat cctcgttctt tctgccctct ctccaaagaa
     7441 tgtatcgctg ccggccgggg cataccctta tccggggtcg ggaccacgta atctcatgat
     7501 attcgagtca gataccgtgg agatggtggc ccggctgatg gcgctttctg cgcgcaccgc
     7561 accgaaagcc cgggggatcg acgtgatccg gacgctgatc gtcaccgggg atgaacggag
     7621 ggtgcttgcc cgggcgatgc gcgagtatgg cgagcggcac aacgccgggt tcttcatccg
     7681 ggatgcgggg aacgtcgagg caagcgacgc ctgcctcatc atcgggtcgc tccttgccga
     7741 cgccgtgggc ctcgactgcg gcgggtgcgg ctacgccacc tgtgcggaga tgctcaaagc
     7801 gcagggtaat ctgcggtcga caccgttctg cggcccgaac tgtgttgtca ggatggccga
     7861 catcggcatc gcggtcggtt cggcggtaaa gaccgcgagc atccacaacg tcgataaccg
     7921 ggtcatgtac acggtcggcg tcggcgcgct ctcgctcggg tggctggaag gctgcggcgt
     7981 cgcctacggc attccgctcc gggcatctgg aaaggatatc ttctttgacc gcacacgctg
     8041 aaacgaagtg acagaatgcc catcatgaag atccgtggag gggatctcga cctcgtcgag
     8101 tacgagttca cctcgttttc ccccggtgag catatccggc cgtgcggcct ccagaaggaa
     8161 taccggctca gcccggttgc cggcaccgtc gtcgtccggg ctccggcgag gatccacctc
     8221 accgtgctcg acatgaaccg gttctctccc gatcaccccg gcgggggcgg gatcggtttt
     8281 gccatcgggg tctactgcac cgccgaggtc gagtgcacgg cctccggcgt cgatatagac
     8341 tacacccgcg aaccgatcct ccgccacttt gtggaggcgt tccgacaggt cgtcggttat
     8401 acgggcggct ttacgatccg ggcgcgcgac caccagtacg agcacgtggg gctcgggtcg
     8461 acgagcacca tattgatcgc ggtcgcaaac gccctcaacg ccgcggtagg gtcgccgctg
     8521 acccccgacg agctccgcct gctcctcggc aacaacttcg tcgaggagac ggcggccggg
     8581 aatgtcgcgt tcgggttcga gaccggtgtc gggcccgcgg cgagcaccca cggcggcatg
     8641 gtggtgatgg gcgacggcct cgcgctcgtc taccggcacc cctttgccgg gggcaagaag
     8701 gtttacatcg ccataccgtc ctccgatata tcgtcggcgg gggagaagga gttcgacctc
     8761 ctgatgaaca aggcccggac ccttgactac cgcgatcgcg cgctgaagtc ctacctcgtc
     8821 ctgatggacc tcatcccggc gctggagcgc ggcgatctcg gggcgatcgg cgacgtcatc
     8881 tgggagatcg agttcagagg atcaaaacgc gccgaggtcg agcaccacgg gtttgagatc
     8941 tatcgctaca tggcggcgct ccgggaggcc ggcctcgagt tcgtcgggat gagctcggtc
     9001 ggcccctcca tagcggtcat caccggccgg ccggaggagg aggtggcggc gatccttgag
     9061 aaggcagggc tccggatcgc gatcgcgacg gcggtggata acgaggggtt gaaggtccac
     9121 c
//
LOCUS       DAOSII010000077         9022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_7308_length_9022_cov_44.605108, whole genome shotgun sequence.
ACCESSION   DAOSII010000077 DAOSII010000000
VERSION     DAOSII010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 9022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9022
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7308_length_9022_cov_44.605108"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..989)
                     /locus_tag="PLV90_06855"
     CDS             complement(177..989)
                     /locus_tag="PLV90_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96872.1"
                     /translation="MNLVTTIAVIVAVLLALLLAGRAGLQVQPAPFPPPAGPAAAPET
                     VPLPEGLPPPVEHFYRTLYGNDLPLIESAVISGRGTMRIGGITFPVRFRFTHETGRNY
                     HHYIENTFFGLPVMKVDEYYVNGTARLELPFGVARGSKIDQGANLALWAEAIWMPAVW
                     ATDPRTHFEPVGEHTAELVVPFGNTEERITLHFDPKTALLQEMQSMRYRGEESEEKTL
                     WLNDVREWSRMDGWLIPVTIAVAWGDEASPWAVFTAEEVVYNGEEPLRTGGV"
     gene            1132..1404
                     /locus_tag="PLV90_06860"
     CDS             1132..1404
                     /locus_tag="PLV90_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF504 domain-containing protein"
                     /protein_id="HOF96873.1"
                     /translation="MRTSRRLLLRFYHDPGYDFSRVEVEYVDRGAPGDRSRVQGDRVV
                     ALDAQYLEVDAGTHAACIPYHRVRRIRYEGETMWQHGDRPGDAGKA"
     gene            1456..1528
                     /locus_tag="PLV90_06865"
     tRNA            1456..1528
                     /locus_tag="PLV90_06865"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1489..1491,aa:Thr,seq:tgt)
     gene            1970..2128
                     /locus_tag="PLV90_06870"
     CDS             1970..2128
                     /locus_tag="PLV90_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96874.1"
                     /translation="MKHINAKGKVFLQTSARIRENVCLLAKEERIPKSEAAEQGVIAA
                     LHARGVEA"
     gene            complement(2446..2622)
                     /locus_tag="PLV90_06875"
     CDS             complement(2446..2622)
                     /locus_tag="PLV90_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96875.1"
                     /translation="MPTKTVVEGKKATPKTPPKGTLTQAEIQASIDRLDADMERWGKG
                     RRYTLAEVEKRLEL"
     gene            complement(2809..3048)
                     /locus_tag="PLV90_06880"
     CDS             complement(2809..3048)
                     /locus_tag="PLV90_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96876.1"
                     /translation="MFDRCPGAAHIRTPTLKIKKCPECGAEVEVFSNDISVRCSECGF
                     TVYNDALSCVQWCKYARECVGEETYQKLVGKKPDR"
     gene            3368..6253
                     /locus_tag="PLV90_06885"
     CDS             3368..6253
                     /locus_tag="PLV90_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOF96877.1"
                     /translation="MRTERFRSLGIPLRELHLVDVYTIATSKRDFSPEELSQIGAQLS
                     NPVVQRYSVDRPTEADFDHAIEVGFLPGVTDNVGATARQTIEDYFGFQFGKGEAVFSS
                     QLYLAAGDLTPESLEKLAGALANPLINRIHIKSREEYGKKGMDAVTPFVHLDDPQAAG
                     AVGLDLSDADLARIGKEGILDPRTGERRGPLALDLAQLHAIRDYFRGLGRMPTDVEIE
                     SLAQTWSEHCKHTIFASAMDDDVPRGLYKTCIQAATNAIRKKRGDADICVSVFTDNSG
                     AIIFDDEHLVTCKVETHNSPSALDPFGGALTGIVGVNRDTIGFGLGAKPCINIYGYCT
                     GDPDTEPALFRGKNRENPILSPRRIFEGVISGVGIGGNCSGIPTPQGWCYFHDRYVGK
                     PLVFAGTVGVMPRAHNGRLLHEKQALPGDLIVMVGGRVGKDGIHGATFSSEALDPASP
                     VTAVQIGDPITQKKFSDVIVREARDLDLYRSITDNGAGGLSCSVAEMAKESGGCHVLL
                     DRVPLKYPGMAPWEIWISESQERMTLAVPEEKIEAFMDLMGKRDVEATVIGTFTDTGR
                     CVVEYDGTTVMDIDLDFLHDGLPEKHLLTLPVETAYPEPDFPCPDRLDDLLLAMLARK
                     NICSKEFISTRYDHTVQGGHVLGPVQGSGRVQTAATLTKVVPGSMKGVGLSQGIFPTY
                     SELDPYRMAIAAIDLAIRGLIAIGIPLDAIALLDNFCWCSSDEPERLWQLKQAAFGCR
                     DGAIAFATPYISGKDSMYNDFSGFDADSRPVKVSVPPTLLITSIGIHPDVTTAVSMDA
                     KFEGDLAYVVGETGEELGGSEYLAHIGIEGGVVPSLDMEAAKARYEKMTGAIAKGLIA
                     SAFPVAHGGLLVALAKVAIAGRVGMNLTIPGGMRPDRYLFSESPGRFVVTVAPGDREA
                     FEEVFGDDAHLLGRVGGTAFRVSAGETLIEQPIRAMKTAYKAPFGGY"
     gene            6256..7071
                     /locus_tag="PLV90_06890"
     CDS             6256..7071
                     /locus_tag="PLV90_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOF96878.1"
                     /translation="MTTVKTAAKALIMSGYGINSEMETAVALMRAGMASDTVHINDLI
                     AGEKSLSDYRLMVFPGGFSYGDDMGAGNACANRIRNNLWSDVKEFLAGDNLALGICNG
                     FQILANLGLVPAFDEDRSREIALMPNRKGVLECRFVTLKPAAENLWTRGISHLYCPVS
                     HGEGNFFCTEETLARIERENMVAFRYCRDDLSPAGGEYPYNPNGALADIAGITSADGK
                     VLGMMPHPERAMEFVNLYDWPTRKERLKREGKPLPTESMNMRFFRNIVAYFGE"
     gene            complement(7209..>9022)
                     /locus_tag="PLV90_06895"
     CDS             complement(7209..>9022)
                     /locus_tag="PLV90_06895"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HOF96879.1"
                     /translation="ISGTIASGENLLPRSLERIDEVLDLVGIELPLPLRFEAYDLDER
                     ILEDVEAAVGREDDLEPAEDAVGDAGEVSVDGGKRQDLVCGRDVGLIDLDFEDRRRIG
                     ALLCKTAGDPDLRGDSSVQFRIFSRKKDIVLCRGAGGLLCRPDAVRVLAGIPIRLPGD
                     ATERSGGRVACNDETDAHNSLQQIYPPVERCFFMRLLSILIALALVAPAAAVFLTAEN
                     AETVGTGLKQVATSGNGTRYEAGSYHVVVLSGTYREMGRQYGALMKDELRAEYAMLRS
                     HFTASGYTEAEIGELARKATTLQAKRMKEIRAGIAETSGLTVEEVDILYEGVPVFFST
                     MNEKAFFSTAGGKAGCSFLAVWGNYTPDGSVVLSRNWDHNDAFLIFKPYYTLAVYNPT
                     DGSNSVATFGPVGLLPETLMNSAGLFIAEDNGESSGSSLEIAGRPQLVGEFFRLMLDY
                     STMDELDAGIMSTRTDCAFIVNAAGADRACSYEETVYDIKCREGTGVIAAANHFVDPS
                     WRLAVPPTEENTVVRYGNLLRLAEESKGSIDGERMVGIRDVLYDDGGATFCHYTVGDL
                     SASSIYQVVFIPETRTLWMKVVDRDWEKVELGQLFSV"
BASE COUNT         1865 a         2812 c         2603 g         1742 t
ORIGIN      
        1 cgtgtttgaa gtagcagtac gaacagaatc cccggcagtc gaggccgggc cggcctccga
       61 gatcgacgtt gagatgcgac atcctgctgg tacttctgtg ttcctcggtt atgaacgttg
      121 cagacccgcc ctccggcgac atcctgcacc gttatgctct ggtgtttgcc ttgcggttac
      181 actccgccgg ttcgaagggg ctcctctccg ttgtaaacga cctcttcggc cgtgaacacc
      241 gcccagggag aggcttcatc gccccaggca actgcgatgg tcacggggat cagccacccg
      301 tccatccggc tccattccct gacgtcgttc agccacaggg tcttttcctc gctctcttcg
      361 cccctgtagc gcatcgactg catctcctgc agcagagcgg tctttgggtc gaagtgcaag
      421 gtgatccgct cctccgtatt gccgaacggg accaccagct ctgccgtatg ctcgcccacg
      481 ggctcaaagt gcgttcgcgg atcggtggcc caaacggccg gcatccagat agcctcggcc
      541 cacagggcga ggttggcgcc ctggtcgatc ttcgaaccgc gggcgacgcc gaacggcagt
      601 tccaggcggg cggtgccgtt gacatagtac tcgtcgacct tcatcaccgg cagcccgaag
      661 aacgtgtttt cgatgtagtg gtgatagttt cgcccggttt cgtgggtaaa ccggaaccgc
      721 accgggaatg tgatcccgcc gatgcgcatg gtgccgcgcc cgctgatcac tgccgactca
      781 atcaacggca gatcgttgcc gtagagggtg cggtagaaat gctccacggg cggaggcagg
      841 ccttccggca acggaaccgt ctccggtgcg gccgccggcc ctgccggcgg tggaaaggga
      901 gcgggttgta cctgcagccc cgcccggccc gcgaggagga gagcgagaag aacggccacg
      961 atgacggcta tcgtagttac gagattcaca tcccctcaca cacctgcaat gctcgacctc
     1021 tgttccgatc ggtacacatc cgtatcgtgc tctcacggtc gaggaggacc ttgtagaatc
     1081 accgttggtc ccgggagggt tttatactct atggagatgc tctaacctca tatgagaacg
     1141 agccgccggc tgctgctccg gttctatcac gatccggggt atgacttctc gcgagtcgag
     1201 gtcgagtacg tcgacagagg ggcgcctggc gaccgctcaa gggtacaggg cgaccgggtc
     1261 gtcgcgctcg acgcacagta cctggaggtg gacgccggga cgcacgcagc gtgtattccg
     1321 tatcaccggg tccggcggat acggtatgag ggggagacca tgtggcagca tggcgatcgg
     1381 ccgggcgatg ccgggaaggc atgaggagga tcagcgccac cttttttacc gcagagaacc
     1441 cacgatataa ggtatgcccc agtggcttag ctggcagagc ggctgacttg taatcagcag
     1501 gtcccgtgtt caaatcacgg ctggggctct tttagatttg ttcgtatctc cagagtgcgg
     1561 gcgctgtgct ccgctggggc ttacctcgat tggattaacg gcaccatttg tgacagaatc
     1621 ctcgttttga catatcttat gagactttat atggatcgag gcacagttgt aattttgttt
     1681 ttgagaaata catatgcctg atcggacaat gtgaacgtat atccctcctt tttacgcctg
     1741 tattgctcct ttttctcctt acgctccata attctcttct cctttccact gtcatgacag
     1801 ttacataacc gtcatgacag attgcgcatg catagctctc tgcgcggggc ctgataagag
     1861 aggtgactaa aaacaggttg cgggggccgg atgttgagag attcgagttg ccgacacctc
     1921 tgtttctgtc accacctctt taagagacta taattgccac ctactgacta tgaagcacat
     1981 caacgcgaag ggaaaggtct ttctgcaaac ctcggctcgt atccgtgaga acgtctgcct
     2041 cctcgcgaag gaggagcgca tacctaagtc agaggctgca gaacagggtg tgatcgccgc
     2101 gctgcatgca cggggcgtcg aggcctgaga gaggcgcatg acgaaatacg aaatcgtaat
     2161 tacagagacg gcagagaggc accttcaggc aatcaagcgt ggagataacg agtatatcaa
     2221 aaaatcattg ataaaataaa attctgcctg ggcgaacacc tgttcacttc gatcaaacaa
     2281 tgcaataaga agcagttgaa agggaaggag aacacccacc ggttacatat tcagaggaag
     2341 tacacggtgt tttataaggt ggtgaaaaca gagcaaaaaa catctgtgga aattcatgag
     2401 gtcatgggtt tcgaggcggc gcacaagaag tacccacaca tcgatttata actccagcct
     2461 cttttctacc tcggccagag tgtatctccg acccttcccc catctttcca tgtctgcatc
     2521 cagcctatcg atactggcct ggatttcagc ctgagtaaga gttcctttag gcggggtctt
     2581 cggggtcgcc ttcttcccct cgactaccgt cttcgtcggc atacagatga tgtcggtatc
     2641 gggaatgata tagttttggg gaggatcact ttcacgcttt gtaggtcttg agccggatga
     2701 tagggaaaat gtccgctcgc cgcacgtatg gacaccctcc gatttgatgg attcgatcaa
     2761 aggatcagac tcagggatgt ttgcatgccg gcacaaccct ctcccctttc agcgatccgg
     2821 ctttttgcct acaagtttct ggtaggtctc ctcaccgacg cattcccgtg cgtacttaca
     2881 ccactggacg caggataagg catcgttgta aacggtaaat ccgcattcgc tgcatctcac
     2941 cgagatgtca ttggagaaga cctcgacctc cgcaccgcac tcggggcatt tttttatctt
     3001 cagcgtcgga gttcggatat gtgcagcacc aggacaacgg tcgaacatta cgcatccttc
     3061 cacggttgat actctcgcaa taatgctttt tgtatacgga gcttcctccg ggtgagtatg
     3121 tcatcgaacc cctttacaga tttcgcgaga gcaggttacg agggcggcat cgccgacggg
     3181 gtgtaacccg ttcagaacca ggccgaagaa gggcgccacg atgcctgccc ggacctcttt
     3241 tgaccccggt acagggccgt gcatcatctc catcgcagct cttttcaccc gatcgcaccc
     3301 atatatatac atggcctctc acgtccaccg gatagaggta cactactcct gcgatccacg
     3361 gttacagatg cgtaccgaga ggttccgctc gctcggcatc cccctgcgcg agctgcacct
     3421 cgtcgacgtc tatactatag cgaccagtaa aagagacttc tcccccgaag aactctcgca
     3481 gatcggcgcg cagctcagca accccgtcgt ccagcgctac tccgtggacc ggccgaccga
     3541 ggctgacttt gatcatgcaa tcgaggtcgg gttccttccc ggagtcaccg ataacgtcgg
     3601 agcgacggca agacagacca tcgaggacta cttcggattc cagttcggca agggggaagc
     3661 ggtcttctca tcgcagctct acctcgccgc cggcgacctg actcccgaat ccctggaaaa
     3721 actcgccggc gccctcgcaa acccgctcat caaccgcatc cacatcaaaa gccgcgagga
     3781 gtacggcaag aaggggatgg atgcggtcac gcccttcgtc catctcgacg acccgcaggc
     3841 ggccggggcc gtgggcctcg atctttccga cgcggacctc gcgcgcatcg gaaaagaggg
     3901 tatcctcgat ccccggacag gagagcggcg gggaccgctc gcgctcgacc tcgcccagct
     3961 ccacgcgata cgggactact tcagggggct cgggcggatg ccgacggacg tcgagatcga
     4021 gtccctcgcc cagacctgga gcgaacactg caagcacacc atctttgcgt cggcgatgga
     4081 cgacgacgtc cctcgggggc tctacaagac ctgcatccag gcggccacca acgccatccg
     4141 caagaagagg ggggacgcgg acatctgcgt ctcggtcttc accgacaact ccggcgccat
     4201 catcttcgac gacgaacacc tcgtgacctg caaggtcgag acgcacaact ccccctcggc
     4261 cctcgacccc ttcggcggcg ccctcaccgg gatcgtcggg gtgaaccgcg acactatcgg
     4321 gtttggcctc ggcgccaaac cctgcatcaa catatacggc tactgcaccg gcgaccccga
     4381 caccgaaccg gccctcttcc gcgggaagaa ccgggaaaac cccatcctct ccccgcgaag
     4441 gatctttgag ggcgtcatca gcggcgtcgg catcggcggc aactgttcgg gcataccgac
     4501 accgcagggg tggtgctact tccacgaccg gtacgtgggt aaacctctcg tcttcgcagg
     4561 cacggtcggc gtgatgccgc gtgctcacaa cgggagactg ctccacgaga agcaggccct
     4621 gcccggcgac ctcatcgtca tggtcggcgg acgggtcggg aaagacggta tccacggcgc
     4681 aaccttctcc tcggaagccc ttgatccggc gagccccgtg accgcggtcc agataggcga
     4741 tccgatcacc cagaagaagt tctcggacgt catcgtcagg gaggcccgcg acctcgacct
     4801 ctaccggagc atcaccgaca acggagcggg ggggctctcc tgctccgtcg ccgagatggc
     4861 aaaggagtcc ggcggatgcc acgtcctcct cgaccgcgtg ccgctgaagt accccggcat
     4921 ggcaccgtgg gagatctgga tatccgagtc gcaggagcgg atgacgctcg ccgtcccgga
     4981 ggagaagatc gaggcgttca tggacctgat ggggaagaga gatgtcgagg caaccgtcat
     5041 cgggacgttc accgataccg gaaggtgcgt cgtggagtac gacggcacga ccgtcatgga
     5101 tatcgacctt gacttcctcc acgacggcct gccggagaag caccttctga cattgcccgt
     5161 ggagacggca tatcccgagc ccgacttccc ctgccccgac cggctcgacg acctcctcct
     5221 tgccatgctc gcacgaaaga acatatgctc aaaagagttc atatcgaccc ggtatgacca
     5281 taccgtccag ggagggcacg ttctcggccc cgtacagggg agcgggcggg tccagaccgc
     5341 tgccaccctg acaaaggtcg tccccggctc gatgaagggt gtcgggctct cgcaaggcat
     5401 cttcccgacg tactcggagc tcgaccccta ccgcatggcg attgccgcga tcgatctggc
     5461 gatccggggc ctgatcgcga taggtatacc gctcgatgcc atcgcactcc tcgacaactt
     5521 ctgctggtgc tcctccgacg agccggagag gctctggcag ctcaaacagg ctgcgttcgg
     5581 atgccgtgac ggtgcgatcg cgttcgcgac accgtacata tcgggaaaag acagcatgta
     5641 taacgatttc tccgggttcg acgcggactc tcgccccgtg aaggtatccg tacccccgac
     5701 gctcctcatc acatcgatag gcatccaccc cgacgttacc accgcagtat cgatggatgc
     5761 gaagttcgag ggcgacctcg cctacgtcgt gggggagacc ggggaggaac tcggcgggtc
     5821 ggagtacctc gcccacatcg gcatcgaggg cggcgtggtc ccatccctcg atatggaggc
     5881 agcaaaggcg cgctacgaga agatgaccgg cgcaatcgca aaagggctta tagcgtccgc
     5941 tttcccggtg gcgcacgggg ggctgctcgt cgcccttgca aaggtcgcta tcgccggacg
     6001 ggtcggcatg aatctcacca tacccggcgg gatgcgcccg gaccgctacc tcttctcgga
     6061 gtcgcccggc cggttcgtcg tcacggtcgc tccgggtgac cgggaagcgt tcgaggaagt
     6121 cttcggcgac gatgcgcacc tcctcggccg cgtcggcggc acggccttcc gggtatcggc
     6181 aggagagacc ctgatcgagc aaccgatcag agcgatgaaa acagcataca aagcgccctt
     6241 cggagggtac tgagaatgac gaccgtcaag accgcggcaa aagccctcat catgagcggc
     6301 tacgggatca actccgagat ggagacagca gttgccctta tgcgggcggg catggcatcc
     6361 gacaccgtcc acatcaacga cctcattgca ggcgaaaaat ccctctccga ctaccggttg
     6421 atggtcttcc ctggcggatt ctcctacggc gacgatatgg gcgccggcaa cgcctgtgcc
     6481 aaccgcatca gaaacaacct ctggagcgat gtaaaggagt ttctcgcggg agacaacctc
     6541 gccctcggaa tctgcaacgg gtttcagatc ctcgcgaatc tcggcctcgt ccccgcgttc
     6601 gacgaggacc gttcccgcga gatcgccctg atgccgaaca gaaagggcgt gctcgagtgc
     6661 cggttcgtca ccctgaaacc cgcggcggag aacctctgga cgcgaggtat ttcacacctc
     6721 tactgcccgg tctcccacgg cgaaggaaac ttcttctgca cggaggagac cctcgcgcgg
     6781 atcgagaggg agaacatggt cgcgttccgg tactgccggg acgacctctc ccccgcggga
     6841 ggggagtacc cctacaaccc gaacggagcg ctcgcagata tcgccgggat aacgtcagcc
     6901 gatggcaaag tgctcggcat gatgccgcac cccgaacggg cgatggagtt cgtgaacctc
     6961 tacgactggc cgacccggaa agaacggctg aagcgcgagg gaaaaccgct ccccaccgaa
     7021 tcgatgaaca tgcggttctt ccgaaatatc gtcgcgtatt tcggagagta acccgatcgg
     7081 ggctcccccg aaatacgtag cgacggcgct tgagaggggg agaagcatgc cggaaaccgg
     7141 cagctcccgg aaggccgcgg gatccgctgc ggtgggatcc cgcccccatt cccggctcgc
     7201 cgcccctctc acaccgagaa caactgcccg agttccacct tctcccagtc ccgatccacg
     7261 accttcatcc agagcgtacg ggtctccggg ataaagacca cctggtaaat cgaggaggcc
     7321 gagagatccc cgactgtgta atggcaaaac gtcgcacccc catcgtcgta cagcacgtcg
     7381 cggatcccga ccatccgttc cccgtcaatc gatcctttgc tctcctcggc gaggcgcagc
     7441 agattgccgt atcgcacgac cgtattctcc tcagtaggag gaactgcaag ccgccacgac
     7501 ggatccacga aatggttcgc ggccgcgatc acgcccgtac cctcgcggca cttgatatca
     7561 tacacggtct cctcatagga gcaggcccgg tcagccccgg ccgcgttcac gataaacgcg
     7621 caatccgtcc gggtcgacat gatcccggca tcgagctcgt ccatagtgga ataatcgagc
     7681 atcaaccgga agaactcccc gaccagttga ggcctgccgg cgatctcaag gctactgccg
     7741 gagctctccc cgttatcctc ggcgatgaaa agcccggcac tgttcatcag ggtctcgggg
     7801 agcaacccta ccgggccgaa cgtcgcgaca ctattgctcc cgtcggtcgg gttgtagacc
     7861 gcgagtgtgt agtatggctt gaagatcaga aaggcatcgt tgtggtccca gttccgggag
     7921 aggactaccg aaccgtccgg ggtgtagttg ccccagacag ccagaaacga gcagccggcc
     7981 ttcccaccgg cggtactgaa gaaggctttc tcgttcatgg tactgaagaa gaccggcacc
     8041 ccttcgtaga ggatatcgac ctcctccacg gtcaggcccg atgtttccgc gataccggcc
     8101 cggatctcct tcatgcgctt tgcctggagc gttgttgcct ttcgggcgag ctcaccgatc
     8161 tccgcctcgg tgtatcccga tgccgtgaag tgcgaccgga gcatcgcata ctcggcccgg
     8221 agttcatcct tcatcagggc gccgtactgc cggcccatct cccggtaggt gccggaaagg
     8281 accacgacat gatacgatcc cgcctcgtag cgcgtgccgt tgccggaggt ggcgacctgt
     8341 ttcagcccgg taccgacagt ttccgcattc tccgccgtga gaaagaccgc cgccgccggg
     8401 gccacgagcg ccagtgcgat gagtatggag aggagtctca tgaagaaaca tctttcaacg
     8461 ggcggataaa tctgctgaag tgagttgtgt gcatctgttt catcattgca ggcaactcgt
     8521 ccaccggatc gctctgttgc atctcccggc agacggatcg ggatgccggc caagacccgg
     8581 acggcatccg gtctgcaaag aagacctccc gcgccccggc ataaaactat atctttcttc
     8641 cgagagaata tccggaactg aacggaggag tcgccacgaa gatcagggtc gcccgctgtt
     8701 ttgcagagga gagcgccgat acgccggcga tcctcgaagt cgaggtcgat caggccgacg
     8761 tcccttccgc agacgagatc ctgacgtttg cctccgtcca cggaaacttc gcctgcatct
     8821 ccgaccgcat cttccgccgg ttcgagatcg tcctccctcc cgacagccgc ctcgacgtcc
     8881 tcaaggatgc gctcatcgag gtcatacgcc tcgaacctga ggggcagggg gagctcaatc
     8941 cctacgaggt cgaggacttc gtcgatgcgc tcgagcgaac gcggcagaag gttctcaccg
     9001 gaggcgatgg tgcctgagat cg
//
LOCUS       DAOSII010000078         8716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_7569_length_8716_cov_55.129777, whole genome shotgun sequence.
ACCESSION   DAOSII010000078 DAOSII010000000
VERSION     DAOSII010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8716
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7569_length_8716_cov_55.129777"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /gene="purF"
                     /locus_tag="PLV90_06900"
     CDS             complement(<1..1430)
                     /gene="purF"
                     /locus_tag="PLV90_06900"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOF96880.1"
                     /translation="MCGIVGIVDAGGVSFPLYYSLYALQHRGQESAGISSFEGTTLYT
                     HRVQGLVAEAFNSNTLQGLRGNVGIGHVRYPTAGSKVPENIQPFNFQYRGLNLAIAHD
                     GNLVNTAELREEYERRGQIFCTTTGTEVIGNIIVEALRTSRSMEDAVRLCMRRLRGSY
                     AVVALLNGTIYAFRDPLGIKPLCIGKIDRGYIVASESVAIDALDGTLVRDVRPGELVR
                     IDEDGLSSIQVATANRRAHCIFEYIYFARADSVIDGRLVYDVRRRIGQNLYDADPVEG
                     DIVCPVPDSGTAYAAGYAERSGIPFVEGLMKNRYMGRTFIMPTQQQREWAVRIKLNTV
                     RGNLKDRRVILVDDSIVRGTTSRRIVNMIREAGAKEIHLCVGSPPIIAPCYLGVDMPT
                     RTELIANSRDVEAVRESIGATSLTYVPFGDLMGAIGCGERDLCTGCLTGCYPVEILGE
                     KSCHCAVDYVAGTHQSNLSTFGKQQTS"
     gene            complement(1440..1622)
                     /locus_tag="PLV90_06905"
     CDS             complement(1440..1622)
                     /locus_tag="PLV90_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37e"
                     /protein_id="HOF96881.1"
                     /translation="MSKGTPSMGKRQKRTHIACRRCGKISFHAQHKVCAACGFGRSSR
                     IRSYRWTEKKAKVPTH"
     gene            complement(1637..1864)
                     /locus_tag="PLV90_06910"
     CDS             complement(1637..1864)
                     /locus_tag="PLV90_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOF96882.1"
                     /translation="MTPRPLDILDQVLNRQPVIISLKGGREIRGILQGYDVHMNLVLD
                     RAEEETGGTAQKLGTLIVRGDNVIYITPSIE"
     gene            1974..2471
                     /locus_tag="PLV90_06915"
     CDS             1974..2471
                     /locus_tag="PLV90_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOF96883.1"
                     /translation="MPKITVKKRHVIRKTQIAGLLDRLRAEIGVSAELFQSDRIERVE
                     TDAPVEIYLVNKKPLLMASGDWAFPTLRGLIEHPISERRVTVDAGAVRFVANGADIMR
                     PGVVSISSDIRQGHPLQVVEERYGKPLAVGVALFDAADMERQEKGKVVKSLHFVGDDL
                     WNLEI"
     gene            2522..2899
                     /gene="sepF"
                     /locus_tag="PLV90_06920"
     CDS             2522..2899
                     /gene="sepF"
                     /locus_tag="PLV90_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HOF96884.1"
                     /translation="MVKKLFDSILGKSPVRDQDEYMELDLASYESVTEEEPASMYIKI
                     ATIADLKDTPRVKDEVYNGNIVIVDIGRLKMDKVTFERVLKDLRDVAKDVNGDIVGLG
                     EQKYVVITPMSVKVSREKIGGGL"
     gene            2899..3426
                     /locus_tag="PLV90_06925"
     CDS             2899..3426
                     /locus_tag="PLV90_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZPR1 zinc finger domain-containing protein"
                     /protein_id="HOF96885.1"
                     /translation="MREACPGTCPACGADIQIVHHRIDIPHFPDLLLVTIACDACGYR
                     HTDTIIPGEREPARWMVRIEEPGDLSTRVVRSTTGTIRIPELGLAVEPGTACEGFVTN
                     VEGVLSRFERAVAIILANPESDEEQEAALRMQEALAAAREVASPFTVILEDPAGNSAL
                     VGEKAEKVLLEEREA"
     gene            3501..3737
                     /locus_tag="PLV90_06930"
     CDS             3501..3737
                     /locus_tag="PLV90_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96886.1"
                     /translation="MARTISVLKWENKQDVDNAVHDIFAEMSERGGLTKDTERAMQHS
                     LLVADPDLTNYFLRTVMEQVPDAMHYFEERGGGA"
     gene            complement(3804..4796)
                     /gene="purM"
                     /locus_tag="PLV90_06935"
     CDS             complement(3804..4796)
                     /gene="purM"
                     /locus_tag="PLV90_06935"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOF96887.1"
                     /translation="MTEHTYREAGVDIDLEARAVRALIDTLTYRRTGAFPMLGKVGHF
                     AGLIDFGPYVLALAVDGVGTKMLVADALCDWRTVGIDCIAMNVNDLYVMNLEPVAFVD
                     YIATDALSVEKMAQIGQGLNEGARQANMNIVGGETATLRGLVNGLDLAGTCLGVQEKE
                     KVVTGESIAPGDVIVGVPSSGIHSNGLTLARRVVEEYASYDTLLANGRTLGEELLTPT
                     RIYAEVLRVTEACTVHGMCHITGGGLLNFSRLSGYGFSITDPLEVPAIFRWLQETGGI
                     GDVEMYRTFNMGMGYAFVVPAASVPVIRSIVPDARVVGKVTEEPGVRFKGIEMR"
     gene            complement(4789..6177)
                     /locus_tag="PLV90_06940"
     CDS             complement(4789..6177)
                     /locus_tag="PLV90_06940"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOF96888.1"
                     /translation="MKFGGTSVGEADCIRRVADIVEPHHRGGDEVALVVSACSGITDQ
                     IIAIADEVVKSMEQPPIGAFLAAMRTRHTRLLGEVAPDYADEVTAVIDDRLTRLQNIL
                     TAVHTLKELTPRSRDYIISFGERLSAPIVAAALRQRGIPSVALDGAEAGIVTTTNHGD
                     ARALPASETGIRNRVVPLLAGSVPVIMGFMGATEQGVVTTLGRSGSDYSAAVIGAGIG
                     ADEIWIWTDVDGVMTSDPRIIRDARVLNDISYLEVMELSYFGAKVLHPRSIEPAMQKD
                     IPIRVRNTFRPDYPGTIVLRDEHREKRVVKAIALIEKVALVNVNGAQMIGRPGVAKTI
                     FSALAEREVNVMMISQGSSEANISLIVDESHLEAAIAALTPIAKQGVVREVTYDRDVA
                     ALAVVGAGMAGTPGTGGRIFSALGRAQINTMMISQGSSEVNVSFVVKGGDGKRALQVL
                     HDEFRLSENSDD"
     gene            6250..7881
                     /locus_tag="PLV90_06945"
     CDS             6250..7881
                     /locus_tag="PLV90_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit A"
                     /protein_id="HOF96889.1"
                     /translation="MLVDEIVDAHVLVIGSGGAGARAAIEASRYGDVVLVSKTIAGKG
                     GCTTMAEGGFNAVLRDQDSTGVHREDTLKGGAYLNDPALVDLLTREAPERLADLVRWG
                     AVFDVTDDRQVAQRPFGGQRFPRTCYAGDRTGHEMMMTLLDRLDATDTQLYQEVSVVD
                     LVTGENGAVAGAVALDRDGDPILFSADATVLATGGGTQAYDISTNSAAGTGDGFAMAY
                     RAGAELIDMEMVQFHPTGAVYPYDARGRLVTEAVRGEGGILLNARRERFMQRYDPDRM
                     ELSTRDVVARAIATEVLEGRGTSRGGVYLDVTHLPAERIETRLPVMLEQFLAYGVDIR
                     REPMEVAPTAHHIMGGLRITPECRTTIAGLFACGEVTGGVHGANRLGGNALADTQVFG
                     KRAGEFAGKTQEQSGRVDPARADGVLRMLDAFFEGAVSPADVKKDLKLTMWNQAGIFR
                     SAPDLRTALGHIRRLADRRLFAASAANLLDCCTVRNMCTTASLIVRCALLRPENRGAH
                     VRRDAEVATDPATSPFGHTYVSLAREGIERREVPV"
     gene            7878..>8716
                     /locus_tag="PLV90_06950"
     CDS             7878..>8716
                     /locus_tag="PLV90_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOF96890.1"
                     /translation="MKAMTIRVSRYDPAHDAEPHFEEYTVRVNEGARVLHALHAIRDE
                     HDPTLAYRYCCGSGQCGSCAVRVDGTPVLACMAEARDGMTVEPLDLPVLKDLTVEMSP
                     VIAKIARICPAPDAHLPAREEIEAIKPLRDCIECLCCVSACPALQVTEFAGPTVLRQE
                     MRLALDPRDSGDRIGDAIEKGLFYCTTCRRCLEVCPKEIDVPGKAIEKLRELANRRGL
                     TLPRHQEVARLVQETGRSVERTKATFLEQVPEVIEPDGPVRGEVGFFVGCMFNGRVPQ
                     TAL"
BASE COUNT         1567 a         2777 c         2838 g         1534 t
ORIGIN      
        1 gacgtctgct gcttcccgaa ggtcgagagg ttggactggt gcgtgccggc cacgtagtcc
       61 acggcgcagt ggcagctctt ctcccccagg atctccaccg ggtagcaccc ggtcaggcac
      121 ccggtgcaga ggtcgcgctc gccgcacccg atcgcgccca tcaggtcccc aaacggcacg
      181 taggtgagcg atgtcgcccc gatgctctca cgaaccgcct cgacgtccct gctgttcgcg
      241 atcagttccg tgcgggtggg catatccacg ccgaggtagc agggggcgat gatcggcgga
      301 gaaccgacac agaggtggat ctcctttgcc cccgcctcac ggatcatgtt cacgatccgc
      361 cgcgacgtcg tcccccggac gatgctgtcg tcgaccagga tcacccgccg gtctttcaag
      421 tttccgcgga cggtgttgag cttgatccgg accgcccact cccgctgctg ctgggtcggc
      481 atgatgaacg tccggcccat gtaacggttc ttcatcagcc cctcgacgaa cgggatgccg
      541 gaccgttcgg cgtagccggc ggcgtaggcg gtcccggagt cggggaccgg gcagacgata
      601 tcaccctcga cggggtctgc atcatagaga ttctgcccga tcctgcgccg cacatcgtag
      661 accagtctcc cgtcgatgac cgagtcggcg cgggcaaagt agatgtactc gaatatgcag
      721 tgcgcccgcc ggtttgcagt cgcaacctgg atcgaggaga gcccatcttc atcgatccgg
      781 acgagctccc ccggacgcac gtcccgaacg agcgtgccgt ccagcgcatc gatcgccacg
      841 ctctccgagg cgacgatgta gccccggtcg atctttccga tacagagcgg tttgatgccg
      901 agcgggtcgc ggaaggcgta gatggtcccg tttaacagtg cgacgacggc ataggagcct
      961 ctcaggcgcc gcatgcagag ccggacggca tcctccatgc tcctggatgt ccggagcgct
     1021 tcgacgatga tgttccctat cacctcggtc ccggtcgtcg tgcagaagat ctgtccccgg
     1081 cgctcgtact cctcccggag ttcggcggtg ttgaccaggt tgccgtcgtg ggcgatcgcg
     1141 aggttgagcc cccggtactg gaagttgaac ggctggatgt tctccggcac cttcgacccg
     1201 gccgtcgggt agcggacgtg gcctataccg acgttgcccc gcagcccctg cagggtgttg
     1261 ctgttgaagg cctcggcaac gaggccctgc accctgtgcg tgtacagggt cgtaccttca
     1321 aaagaggaga ttcccgcgct ctcctgcccc cggtgctgga gagcatagag ggaatagtac
     1381 agcggaaaag agacaccgcc agcatcgacg atgccaacga taccacacat gatccagctc
     1441 tagtgcgtcg gtacctttgc tttcttctcc gtccagcggt aactgcggat cctgctgctc
     1501 ctgccaaacc cgcaggctgc acagaccttg tgctgtgcgt ggaacgagat tttgccgcac
     1561 cgcctgcagg cgatatgggt acgtttctgc cgtttgccca tggagggtgt gccttttgac
     1621 atgaactctc acctactcat tcaattgaag gggttatgta aattacgttg tcgccgcgga
     1681 cgatcagcgt accgagtttc tgcgctgtgc cgccggtctc ttcttctgcc ctgtccaata
     1741 ccagattcat gtgaacgtcg tatccctgga ggataccccg gatctccctt ccacctttga
     1801 ggctgatgat gacgggctga cgattcagta cctgatctaa aatatccaac ggtcttggcg
     1861 tcatacaatt accctttgcc gtcctttctg gagtaatata tttgagtgag gaagctactt
     1921 aaatataacc gcactgttgc ggggagcatg ttgcacccca gggtaagagt tccatgccga
     1981 agatcaccgt gaaaaaacgc cacgtcatca gaaaaacgca gatagcaggg cttctcgacc
     2041 ggctccgcgc cgagattgga gtctcggccg aactgttcca atccgaccgg atcgagcggg
     2101 tggagacgga tgcccccgtc gagatctacc tcgtcaacaa aaaaccgctc ctgatggcct
     2161 caggggattg ggccttcccc accctgcggg gcctcatcga gcacccgatc tccgagcgcc
     2221 gggtgacggt cgatgccggc gcggtcaggt tcgttgcaaa cggcgcggac atcatgcgcc
     2281 ccggtgtcgt ctcgatatcg tcggatatac gtcaagggca cccgctacag gtcgtcgaag
     2341 aacgttacgg taaaccgctc gctgtcgggg ttgctctctt cgatgccgcc gatatggagc
     2401 ggcaggagaa gggcaaggtg gtcaaaagcc tccactttgt cggggatgat ctctggaatc
     2461 ttgagatctg acgcggaaat ctagagatac tattaaataa aattctttcc tcattttctc
     2521 tatggttaaa aagctctttg acagcatcct cggtaaaagc cccgtcaggg atcaggacga
     2581 atacatggag ctcgacctcg cctcctacga gagcgtaacc gaagaagaac cggcatccat
     2641 gtacatcaag atcgccacca tcgctgacct caaggatacc ccccgcgtca aagacgaggt
     2701 ctacaacggc aatatcgtca tcgtcgacat cgggcggctg aagatggaca aggtgacctt
     2761 cgagcgggtc ttaaaggacc tccgcgacgt ggcaaaggac gtgaacggcg acatcgtcgg
     2821 cctcggcgag cagaagtacg tcgtcatcac gccgatgtcc gtcaaggtct cccgcgagaa
     2881 gatcggcgga ggcctgtagt gcgggaggcc tgccccggga cctgcccggc gtgcggggcc
     2941 gatatccaga tcgtacacca tcgcatcgat attccccact tccccgacct cctgctcgtc
     3001 acgatcgcct gcgacgcctg cggctaccgg cacaccgaca ccatcatccc gggggaacgc
     3061 gagcccgccc ggtggatggt gcgcatcgag gagccgggcg acctttccac ccgggtggtg
     3121 cggagcacga cggggacgat ccggatcccc gaactcgggc ttgcggtcga gcccgggacc
     3181 gcctgcgagg ggttcgtcac gaacgtcgag ggggtcctct cccggttcga gcgggcggtg
     3241 gcgatcatcc tcgcgaaccc cgagagcgac gaggaacagg aggccgcgct ccggatgcag
     3301 gaggcgctcg ccgccgcacg agaggtagcc tccccgttca cggtcatcct cgaggacccg
     3361 gccgggaaca gcgcgctcgt cggagagaag gccgaaaagg tgctcctcga agagagagag
     3421 gcctgatacc tcttttttgc cccatcccgg ggtaagcctc aaatataacc acccccatat
     3481 gcagcgccgg gtgaaaaaga atggcacgaa ccatatcggt gctgaagtgg gagaacaagc
     3541 aggatgtcga caacgccgtc cacgacatat tcgcggagat gagcgagcgg ggcgggctca
     3601 ccaaggatac cgagcgggcg atgcagcact cgctcctggt ggccgatccc gacctcacga
     3661 actacttcct gcggacggtc atggaacagg tccccgacgc gatgcactac ttcgaggagc
     3721 gcggcggcgg ggcgtgagca ccccggggga gggatggtcc cggacagcgc cgcacgaaag
     3781 aacgcggggg aatacccccg cattcaccgc atttcgatgc ccttgaaccg gaccccgggc
     3841 tcctccgtca ccttcccgac cacccgggca tcggggacga tcgatcggat cacgggcacg
     3901 cttgccgccg gcacgacgaa ggcatagccc atgcccatgt tgaaggtgcg gtacatctcc
     3961 acgtcgccga tgccgccggt ctcctgcagc cagcggaaga tggccgggac ctcgagcggg
     4021 tcggttatgg agaacccgta ccccgagagc cttgaaaagt tcaggaggcc gccgccggtg
     4081 atgtggcaca tgccgtggac ggtgcaggcc tccgtcaccc ggagcacttc tgcgtatatc
     4141 cgggtcggcg tcaggagttc ttcgccgagg gttctgccgt ttgcaagaag ggtgtcgtaa
     4201 gatgcgtact cctcgacgac ccggcgggcg agcgtcagac cgttgctgtg gatgccgctt
     4261 gaggggacgc cgacgatcac gtcgccgggg gcgatcgatt caccggtgac gaccttctcc
     4321 ttctcctgga cccctagaca cgtccccgca aggtcgaggc cgttcacgag ccccctgagc
     4381 gtcgcggtct cgccgccgac gatgttcatg ttcgcctgcc gggcgccctc gttcagaccc
     4441 tgcccgatct gggccatctt ctcgaccgag agggcgtcgg tcgctatgta gtcgacgaac
     4501 gctacgggtt cgaggttcat cacgtagagg tcgttgacgt tcattgcgat gcagtctatc
     4561 ccgaccgtcc gccagtcgca aagcgcgtcg gcaacgagca tcttcgtgcc gacgccgtcg
     4621 accgcaagag caaggacata cggaccgaag tcgatcagac cggcaaagtg cccgaccttc
     4681 ccgagcatcg ggaatgcgcc ggtcctccgg taggtgaggg tatcgatcag cgcccgcacc
     4741 gcccgggcct cgaggtcgat atcgaccccc gcctcgcggt aggtatgttc agtcatcgga
     4801 gttctccgaa agccggaatt cgtcgtgcag tacctggaga gcccgtttgc cgtcgccgcc
     4861 cttcacgacg aacgagacgt tcacctccga ggatccttgc gatatcatca tggtgttgat
     4921 ctgcgcccgc ccgagcgccg agaagatccg gccgcccgtc cccggggtgc cggccatccc
     4981 cgcgcccacg acggcaagcg cggcgacgtc ccggtcgtag gtgacctccc gcacgacgcc
     5041 ctgcttcgct atcggggtga gggccgcgat agcggcctcg aggtgggact catcgacgat
     5101 gagggatatg ttggcctcgc ttgagccctg cgatatcatc atgacgttca cctcccgctc
     5161 cgcgagcgcg gagaagatcg tcttcgcgac gcccgggcgg ccgatcatct gcgcaccgtt
     5221 gacgttgacc agggccacct tctctatcag ggcgatggcc ttgaccaccc gcttctcgcg
     5281 gtgttcgtcg cggaggacga tcgtcccggg gtagtcgggc cggaatgtgt tccggacccg
     5341 gatcggtata tccttctgca tcgcgggttc gatcgaccgc gggtgaagga ccttcgcgcc
     5401 gaagtaggag agttccatca cctcaaggta ggatatgtcg ttgagaaccc gggcatctct
     5461 gatgatacgg gggtcagatg tcatcacccc gtcgacgtcg gtccagatcc agatctcgtc
     5521 ggcaccgatc ccggcgccga tgacggcggc cgagtagtct gagccgctcc gcccgagggt
     5581 ggtgacgaca ccctgctcgg tcgctcccat gaagcccatg atcaccggga cggacccggc
     5641 aagcagcggc acgacccggt tccggatgcc cgtctcgctc gcgggcaggg cgcgggcgtc
     5701 tccatggttc gtcgtcgtga cgataccggc ctctgcgccg tcgagggcga ccgacgggat
     5761 accccgctgc cggagggcgg ccgcgacgat cggggcggag agcctctcgc caaaggagat
     5821 gatgtagtcg cgggaccggg gagtcagctc cttcagggtg tgcacggcgg tgagtatgtt
     5881 ctgcagccgg gtgagccggt cgtcgatgac ggccgtcacc tcatcggcgt agtcgggggc
     5941 cacttccccg aggagccggg tgtgccgggt ccgcatcgcg gcaaggaacg ccccgatggg
     6001 gggctgttcc atgcttttta cgacctcatc ggctatcgcg atgatctggt cggtgatccc
     6061 cgagcacgcc gatacgacca gggcgacctc gtcgcctcct ctatgatgtg gttctacgat
     6121 atctgcaacc cgccggatac agtcggcttc cccgacggat gtgccgccaa acttcattaa
     6181 aagcctcatt ctggctcacg tgtcccctgc aattacttta tggtagtata caacgcacag
     6241 actaataaga tgcttgtaga cgagatagtg gacgcgcatg tgctggttat cggaagcggc
     6301 ggcgccggcg cgcgggctgc gatcgaggcc tcgcgatacg gcgacgtggt cctggtctcc
     6361 aagaccatcg ccggaaaagg cggatgtacg acgatggcag aaggcggatt caacgccgtg
     6421 ctgcgggatc aggactcgac cggcgtccac cgcgaggata cgctcaaggg gggggcatac
     6481 ctcaacgatc ccgcgctcgt ggatctcctc acccgggagg cgccggagcg gctcgcggat
     6541 ctcgtccggt ggggggcggt attcgacgtc acggatgacc ggcaggttgc gcagcggccg
     6601 tttggagggc agcggttccc ccggacctgc tacgccgggg accggaccgg ccacgagatg
     6661 atgatgaccc tccttgatcg cctcgacgcg acggatacgc agctctacca ggaggtctcg
     6721 gtcgtcgacc tggtaacggg cgagaacggt gcggtcgcgg gcgcggtcgc tctcgaccgg
     6781 gacggggatc ccatcctctt ctcggccgac gcgacggtgc ttgcgacggg gggcgggacg
     6841 caggcctacg acatatccac gaactccgcc gccgggaccg gggacgggtt tgccatggcc
     6901 taccgcgcag gggcggagct gatcgatatg gagatggtcc agttccaccc gacgggcgcg
     6961 gtctacccct acgacgcccg cggccggctg gtgacggagg cggtccgggg cgaagggggg
     7021 atcctcctga acgcgcgaag ggaacggttc atgcagcgct acgatcccga ccggatggag
     7081 ctctcgacac gggacgtagt ggcccgggcg atagcgaccg aggtgctgga agggcgcggg
     7141 acgagccgcg gcggggtcta cctcgacgtg acccatctcc cggcggagcg gatcgagacc
     7201 cggctcccgg tgatgctcga gcagttcctc gcctacggcg tggatatacg gcgggagccg
     7261 atggaggtgg cgcccaccgc ccaccacatc atgggcgggc tccggatcac cccggagtgc
     7321 cggacgacga tcgccggcct cttcgcctgc ggagaggtga ccggcggggt gcacggcgca
     7381 aaccgtctcg gcgggaacgc gcttgcggat acgcaggtct tcgggaaacg ggccggcgag
     7441 ttcgccggga agacgcagga gcagagcggc cgggtcgacc ccgcccgggc cgacggggtg
     7501 ctccggatgc ttgacgcctt cttcgagggg gccgtgagcc ccgcggatgt gaagaaagac
     7561 ctgaaactga cgatgtggaa ccaggccggg atcttccgga gcgctcccga cctccggacg
     7621 gctctcgggc atatcaggcg gcttgccgac cggcgtctct tcgccgcgtc ggccgcaaac
     7681 ctccttgact gctgcacggt ccggaacatg tgcaccacgg catcccttat cgtccggtgc
     7741 gccctgctcc ggccggagaa ccggggagcc cacgtccggc gggacgccga ggtcgcgacc
     7801 gatccggcga cgtcgccgtt tgggcacacc tacgtctccc tcgcccgcga ggggatcgag
     7861 cggcgggagg tgccggtatg aaggcgatga ctattcgggt ctcccgttac gacccggccc
     7921 acgacgcgga gccgcacttc gaggagtata cggtccgggt caacgaaggc gcccgggtgc
     7981 tccacgccct ccacgcgatc cgcgatgagc acgacccgac gctcgcctac cgctactgct
     8041 gcgggtcggg gcagtgcggg agctgcgccg tcagggtgga cgggacaccc gtccttgcct
     8101 gcatggcgga ggcccgcgac ggcatgacgg tcgagccgct ggaccttccg gtcttgaaag
     8161 acctgacggt ggagatgagt ccggtgatag caaagatcgc ccggatctgc ccggcaccgg
     8221 atgcgcatct cccggcacgg gaggagatcg aggcgatcaa gccgctccgg gactgtatcg
     8281 agtgcctctg ctgcgtctcg gcctgcccgg cgctccaggt gacggagttt gcggggccga
     8341 cggtgctccg gcaggagatg cgcctcgccc tcgatccccg ggactcgggg gaccggatag
     8401 gcgatgcgat cgagaagggg ctcttctact gcacgacctg caggcgctgc cttgaggtct
     8461 gcccgaagga gatcgatgtc ccgggcaaag ccatcgagaa gctccgggaa cttgcgaacc
     8521 ggcgcgggct gaccctcccc cgccaccagg aggtggcgcg gctggtccag gagacgggcc
     8581 ggagcgtcga gcggacaaaa gccaccttct tagagcaggt gccggaggtg atcgagcccg
     8641 acggccccgt ccgcggcgag gtggggttct tcgtcgggtg catgttcaac ggccgggtgc
     8701 cgcagacggc cctcga
//
LOCUS       DAOSII010000079         8322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_8004_length_8322_cov_46.141164, whole genome shotgun sequence.
ACCESSION   DAOSII010000079 DAOSII010000000
VERSION     DAOSII010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8322
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8004_length_8322_cov_46.141164"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(779..2485)
                     /gene="mcrA"
                     /locus_tag="PLV90_06955"
     CDS             complement(779..2485)
                     /gene="mcrA"
                     /locus_tag="PLV90_06955"
                     /EC_number="2.8.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme-B sulfoethylthiotransferase subunit
                     alpha"
                     /protein_id="HOF96891.1"
                     /translation="MAKIERSQKLFLKSLKEKFQGQDVESETAEFYKFNGVRQSPRKV
                     EFMKASLAAEMDRGISMYDPERCHLGGIPMGQRQLMTYEVSGTGVFVEGDDLHFVNNS
                     AMQQMWDDIRRTVIVGMDLAHQTLQKRLGKEVTPETINEYLHILNHAMPGAAVVQEHM
                     VETHPGLVDDCYVKVFTGDQELADDIEPQFVLDIEKLFPAKQAEELKAEVGKSMYQAI
                     HIPSTVSRTCDGGTTSRWSAMQIGMSFIAAYRMCAGEAAVADLSYAAKHAGVVQMASH
                     LPARRARGPNEPGGIKFGLFSDIIQANRKYPSDPARASLEVVGAGTMLYDQIWLGSYM
                     SGGVGFTQYATAAYTDNILDEYTYYGMDYIKDRYKVDWRNPSPKDKVRPTQEIVNDMA
                     TEVTLNAMEQYEQFPTMMEDHFGGSQRAGVIAAASGLTTSIATGNSNAGLNAWYLSML
                     LHKDGWSRLGFFGYDLQDQCGSANSLSIRGDEGAIGEVRGPNYPNYAMNVGHQGEYAA
                     IVGGAHYGRGDAFCFDPRVKICFADPALKFDFAEPRREFAKGAIREFMPAGERSLIIP
                     SR"
     gene            complement(2491..3255)
                     /gene="mcrG"
                     /locus_tag="PLV90_06960"
     CDS             complement(2491..3255)
                     /gene="mcrG"
                     /locus_tag="PLV90_06960"
                     /EC_number="2.8.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme-B sulfoethylthiotransferase subunit
                     gamma"
                     /protein_id="HOF96892.1"
                     /translation="MAYKPQYGPGTSIVAANRRKQMNPGQKLEKVRSVTDEDIVLILG
                     HRAPGSAYPSAHPPLGEQQEPDCPMRRIVKPTEGASAGDRVRYIQFADSMFNAPSQPY
                     QRTYTEMYRFRGIDPGTLSGRQIVECRERDLEKYAKELIETELLDPARTGIRGATVHG
                     HSLRLAENGMMFDMLQRSVLGEDGVVRYVKNQIGEPLDRAVAVGKPLDEKWLKAHTTI
                     FHSLVGTPYRDDTEYIEYVQRIHSLRTKYGFMPREE"
     gene            complement(3466..4770)
                     /gene="mcrB"
                     /locus_tag="PLV90_06965"
     CDS             complement(3466..4770)
                     /gene="mcrB"
                     /locus_tag="PLV90_06965"
                     /EC_number="2.8.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme-B sulfoethylthiotransferase subunit
                     beta"
                     /protein_id="HOF96893.1"
                     /translation="MAKYTETIDLYSDDGKLLKGGVTLDRISPLVNPATSKIIDLTKR
                     TINVNLGGIQDALKTGKLGKGKIKGRELDLPIMENKDAIVARIKDMIQVTEGDDTEIM
                     EFNGGKLQLVKVPAKRLANAATYDAAITSVAAATTYAIVEEFGIDAFNASTVKAACWG
                     GYPHTQDLEGALVTSILNIPQNNEGLGYALRNIPVNHTVMMTGRNALQGAALASTLET
                     AGVFEMGGAVGPFERAMLLAYAYQGLNANNMVYDLVKANGQTGTVGTVVQSLVERAIE
                     DKVIAPGKKGGYFQFYDTKDPMLWNAYTAAGTMAATIVNCGAGRFAQAVSSTLLYFND
                     LLEHETGLPGCDYGRVMGTAVGFSFFSHSIYGGGGPGIFNGNHVVTRHANGVAIPCVV
                     AAAALDAGTQMFSPESTSKIFADTYGKIDVFNKPIDQIANGA"
     assembly_gap    5756..5855
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6247..6663
                     /locus_tag="PLV90_06970"
     CDS             6247..6663
                     /locus_tag="PLV90_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HOF96894.1"
                     /translation="MAKKITLNMITQRSVEEGVGIVKGKMTPDYFEACSIIEMHDDDV
                     RALGLVPNQLVRVTSECGSVVVKSIRGRQALYPGLAFIPQGVYANQIVPARTQATGEP
                     QYSGFPVTVEPVAPGERRKSTLELVQSAVGMWKGDQ"
     gene            6663..8030
                     /locus_tag="PLV90_06975"
     CDS             6663..8030
                     /locus_tag="PLV90_06975"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HOF96895.1"
                     /translation="MAKKIENVGCPFCGVCCDDLVVTVSDDGKKILEVENACIIGSTV
                     FHRAMEDRHALPRLRQPDGTFKEISYDEAIDYTAKVLHNAKKPLIYGFGSTNCEGMAA
                     AAKVAEKAGAVLDNCASICHGSSFLAIFDNGYPSCTLGETKNRADVIVYWGANPSHAH
                     PRHMSRYSIFPRGFFTGKGHKGRKIIVIDPRHTDTAQVADIHLQVQQGHDYDLFDAFR
                     TVLRGHDIPDVVAGVKKEQIVEAVDIMKKARFGTIFYGMGLCHSDGRNHNVDIAISLT
                     RDLNDFTKWTIMAMRGHYNITGPGAVWSWQYGFPYCLDLTKRDIAHMNPGETSSVDLA
                     MRDEVDAFINIGTDAAAHFPIEPVKHLRKHPWIAIDPHPNMATEISDLHIPVGIVGVE
                     VPGIVYRMDNVPIQYRKVIDPPEGVISDEELFERIHLRLCELEAEDAVNVPAKAAPEG
                     VRAGE"
     gene            8034..>8322
                     /locus_tag="PLV90_06980"
     CDS             8034..>8322
                     /locus_tag="PLV90_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOF96896.1"
                     /translation="MGELIVKNGFVFDPLQGIKGDRMDVGVRDGKIVETNTLKNPKEI
                     DAAGMTVMAGGVDIHAHVVGPKVNVGRLFRPEDKLFKSPLKRGNRMEMGFSV"
BASE COUNT         1587 a         2412 c         2637 g         1586 t
ORIGIN      
        1 aggcagcgcc cgagccactg cccctgcccc cctctggggc gggggtgccg gcagggcaca
       61 ggaggacctc accggcaaag aggcgcgggc ggggcggggg gccggcgaga agtaagatgg
      121 ttgaagtgtg agggacaggg gagggggaac aagttccccc gccccgtcgg cccctccccc
      181 cgtggcgata tccccacggt ccacagcatg gggagatcct ggagaagagc cggatacggt
      241 ttcccgccgg ctataccccg ggcacggctg ttcccctgat atcgccacgc actgccaccg
      301 cccccgcggc gggggcgggg gaggagcgcc gaaggcgcgg gtggggtggg ggtgccagaa
      361 gggtatgggt attcttctac gaaggcgcgg gcggggtgga gtcgccggca gaagcgtatg
      421 ggtatccttc tgcgaaggcg aggtcggggt aaaggctgcc ggcaaggaac gtgcggtgcc
      481 tacctgcagg ggaggtgcag gccgggtgga gggccgccgg agaagggctc ccgtgaggaa
      541 gatcgccccg tatcggcccc tgcgcgcctc ccggcggccg gggcgggcat ctctccggtc
      601 gaggaggccg atcgtgcgaa taatgtgcga ggaatgagca gaacttgtga tattatctca
      661 tgttttcata atacgtgggc gagtgagaac cactcgctcc acctgttccg tctgatcggc
      721 aggaatgttt gcgatcggga agttgtgagg acgtgcaact aaaaagggta gggaggggtt
      781 accgggaggg gatgatgagt gagcgctcgc cggcaggcat gaactcgcgg atcgcaccct
      841 tcgcgaactc gcggcggggc tcggcgaagt cgaacttcag ggccgggtcg gcgaagcaga
      901 tcttcacccg cgggtcgaaa cagaacgcat cgccgcggcc gtagtgcgcg ccgccgacaa
      961 tcgcggcgta ctcgccctgg tggccgacgt tcatcgcgta gttcgggtag ttcgggccgc
     1021 ggacctcgcc gatcgcgccc tcgtccccac ggatggagag cgagttcgcg gacccgcact
     1081 ggtcctggag gtcgtagccg aagaagccga gacgcgacca tccgtccttg tgcaggagca
     1141 tcgagaggta ccaggcgttc aggccggcgt tcgagttgcc ggtcgcgatg gaggtcgtga
     1201 ggccggatgc ggcggcgatg acgccggcac gctgggaccc gccgaagtgg tcctccatca
     1261 tggtcgggaa ctgctcgtac tgctccatgg cgttgagcgt gacctcggtc gccatgtcgt
     1321 tgacgatctc ctgggtcggc ctgaccttgt ccttcgggct cgggtttctc cagtcgacct
     1381 tgtacctgtc cttgatgtag tccataccgt agtaggtgta ctcgtcgagg atgttgtcgg
     1441 tgtaggccgc ggtcgcgtac tgggtgaacc cgacaccgcc ggacatgtag gagccgagcc
     1501 agatctggtc gtagagcatg gttccggcgc cgacgacctc gagggaggcc cgggcggggt
     1561 cgctcgggta tttccggttc gcctggatga tatcggagaa gaggccgaac ttgatgccgc
     1621 cgggctcgtt cgggccacgg gcacgccggg caggcaggtg ggaggccatc tggacgacgc
     1681 ccgcgtgctt tgccgcgtag gagaggtcgg cgacggccgc ttcaccggcg cacatgcggt
     1741 aggcggcgat gaaggacata ccgatctgca tcgcggacca ccgggaggtc gttccaccgt
     1801 cgcaggtcct cgataccgtg gacgggatgt ggatcgcctg gtacatgctc ttgccgacct
     1861 cggccttgag ttcctcggcc tgctttgcgg ggaagagctt ctcgatgtcg aggacgaact
     1921 ggggctcgat gtcgtcggcg agttcctggt cgccggtgaa gaccttgacg taacagtcgt
     1981 cgacgaggcc ggggtgggtc tcgaccatgt gctcctggac gacggccgca ccgggcatgg
     2041 cgtggttcag gatatggagg tactcgttga tcgtctcggg ggtgacttcc ttaccgagac
     2101 gcttctgcag ggtctggtgg gcaaggtcca tcccgacgat gacggttctc ctgatatcgt
     2161 cccacatctg ctgcatagca gagttgttga cgaagtgcag gtcgtcgccc tcgacgaaga
     2221 cgccggtgcc gctgacctcg taggtcatca gctggcgctg gcccatcggt ataccgccca
     2281 ggtggcagcg ctcggggtcg tacatggata tgccgcggtc catctcggcg gcaaggctcg
     2341 ccttcatgaa ctccaccttg cgaggagact ggcgaacacc gttgaacttg tagaactcgg
     2401 cggtctcgga ctcgacgtcc tgtccctgga acttctcctt gagggatttc aggaacagct
     2461 tctgggatct ctcaatcttt gccattgtga tcactcttct ctgggcatga agccgtattt
     2521 cgtccgcagc gagtggatgc gctggacgta ttcgatgtac tcggtgtcgt cacggtaggg
     2581 agtccctacg agcgagtgga agatcgtcgt gtgggccttg agccacttct cgtcgagcgg
     2641 tttgccgacg gcgaccgcac ggtcgagggg ctcgccgatc tggttcttga cgtaacggac
     2701 gacgccgtcc tcgccaagga cgctcctctg gagcatgtcg aacatcatgc cgttctcggc
     2761 aagacggagc gagtggccgt gcacggtcgc gccgcggatg ccggtgcggg ccgggtcgag
     2821 gagttcggtc tcgatgagtt cctttgcgta cttctcgagg tcgcgctcgc ggcactcgac
     2881 gatctggcgg ccggagagcg tgccggggtc gatgccgcgg aagcggtaca tctcggtgta
     2941 ggtccgctgg tagggctgcg agggggcgtt gaacatcgag tcggcgaact ggatgtagcg
     3001 gacgcggtcg ccggcactgg cgccttcggt gggcttgacg atcctgcgca tggggcagtc
     3061 gggctcctgc tgctcaccga gcggcgggtg ggcgctcggg tatgccgagc cgggggcacg
     3121 gtggccgagg atcagcacga tatcctcatc cgttacggaa cgaaccttct caagcttctg
     3181 gccggggttc atctgcttgc gccggtttgc ggcgacaatc gacgtcccgg gaccatactg
     3241 gggcttgtat gccattgtat cattcacctc agattgattg ttatcgtatc cattttcggt
     3301 tgatttgaac gactttctgc catatctccg ggtgaccgcc ggaccttccc cgtaccgggg
     3361 gtccgcaagg ccgataggcg gtgcaccgca cgggaagctc cggatcggtg cccgataggc
     3421 ccgggttgcg gagatgcggc gttcgtcatt cggatctccg ctcgttcagg ctccgtttgc
     3481 gatctggtcg atcggcttgt tgaagacgtc gatcttaccg taggtgtcgg cgaagatctt
     3541 cgatgtgctc tcgggcgaga acatctgggt tccggcgtcg agcgccgctg cggcgaccac
     3601 gcacgggatg gcgacgccgt ttgcgtgccg ggtcacgacg tggttgccgt tgaagatacc
     3661 ggggccgccg ccgccgtaga tcgagtggct gaagaacgag aacccgacgg cggtacccat
     3721 gacacggccg tagtcacagc cggggagacc ggtctcgtgc tcgagcaggt cgttgaagta
     3781 gaggagcgtc gaggagaccg cctgggcgaa ccggccggca ccgcagttga cgatggtggc
     3841 tgccatggtt ccggcggcgg tataggcgtt ccagagcatg gggtccttcg tgtcgtagaa
     3901 ctggaagtag ccgcccttct tccctggagc gatgactttg tcctcgatag cgcgctcgac
     3961 aaggctctgg acgaccgtac cgaccgttcc ggtctggccg ttcgccttga cgaggtcgta
     4021 gaccatgttg ttcgcgttca ggccctggta ggcgtaggca agcagcatgg cgcgttcgaa
     4081 cggcccgacg gcgcctccca tctcaaagac gccggcggtc tcaagcgtcg atgcaagtgc
     4141 agcgccctgc agggcgttcc tgccggtcat catgacggtg tggttgaccg ggatgttccg
     4201 gagcgcgtag ccgaggcctt cgttgttctg ggggatgttg aggatcgacg tgacgagcgc
     4261 accctcgagg tcctgcgtgt gcgggtagcc gccccagcag gctgccttga cggtggatgc
     4321 gttgaaggcg tcgatgccga actcctcgac gatcgcgtag gtggtcgcgg ctgcgaccga
     4381 cgtgatcgcg gcgtcgtaag tggcggcgtt tgccaggcgc tttgccggga ccttgacgag
     4441 ctggagtttt ccaccgttga actccatgat ctcggtgtcg tcgccctcgg taacctggat
     4501 catgtccttg atccgggcga cgatggcgtc cttgttctcc atgatgggga ggtcgagctc
     4561 tcttcccttg atcttgccct tcccgagttt tccggtctta agtgcgtcct ggatgcctcc
     4621 aaggttcacg ttgatcgttc tcttggtcag gtcgatgatc ttacttgtgg ccgggttgac
     4681 cagcgggctg attctgtcga gggtgacgcc gcccttgagc agcttcccgt cgtcagaata
     4741 gagatcgatt gtttccgtgt atttagccat agtatttcat ccttttaccc ttgttcaatg
     4801 cattccgttc gtgaaagagg ccggagcgtt cacatgcccc ggttctctga aagccgctac
     4861 tgcacgtccc agaagggact ataagaatag aaggagggtt attaataaaa tttacgaatt
     4921 tagatcgaat tttagcataa ataataatat ttattcagat tttattctga tcgaattatt
     4981 ttaaaacagg gggtttatgg ctcagatagt tgagcgttgt acttaaaaag ggttttggag
     5041 agaggccacc ctcgaccagc aaccgggaga ggtcagcaga gagagactta ttaaataata
     5101 aaatagctaa acaaagttac aatatgtgct attattgctc catgaaggag atcaaaagta
     5161 cctgactgta tacattacac tcaaagatat ataatgctac tcaaaatggg gaggtttctc
     5221 gaaaccccga atcgtcagac acctatttta caaacgcgcc tgatttcgat cgggaataaa
     5281 acggatacgg tgagcatcta cccgccggca acccatcccc ggtacgccac gggcagggcg
     5341 ggaagatatc gccccgcact gcgccccggg aagggatcga ggccgccggc ccggggggca
     5401 ccccccaaga gagatcttcc gcgtgagcag gggctccctg acccacgtcc gagctatcca
     5461 tcggcgggcg gagtaacccg gcccggtgca gtgagccccc cgcacgccag aaagaagggg
     5521 agcaacccgc agccggctcc accggcgatc aaccccgcaa acctcatccg tagcctgtct
     5581 tcccaccggc caatctcctc gatgcacccg ggcggcggat gttgtgccgg ccaatccccg
     5641 cccgaccggg ttaaccggat atcagaccgg aagcacccgg gcagccggtg aaaataccac
     5701 ctcgcgattt ttctgcaacg tcaccgcaac cggggccggt ataagttaat tatgannnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctcgc gatttttctg caacgtcacc
     5881 gcaaccgggg ccggtataag ttaattatga taaattaaca atttaaacta aatctggttg
     5941 cctgatatca cttacaaatg cggaatagtt aaatataaac cattacaagt tatgataaac
     6001 cgatctctcc ggtgctccgc cgggagagtc tcagtcaacc cctgatggac agccgatctg
     6061 cgtatccccc ggtttctggc agaccttccc cgtgcaggaa ggcccggcat gccgggtagg
     6121 ggcgcccccc ggggaggagg ctctcgcaac agaatgaacg gcctgcatcc gggccggagc
     6181 accacgttcc ggggccggat ctccaggaag agaaccggtc ctgcaggtac acaacgaggc
     6241 agaaccatgg cgaagaagat tacgctgaac atgatcaccc agcgctccgt agaagaaggc
     6301 gttgggatag taaagggcaa gatgaccccg gattacttcg aggcctgctc catcatcgag
     6361 atgcacgacg acgacgtcag agcgctcggt ctggtcccaa accagctcgt acgggtgacg
     6421 agtgaatgcg gaagtgtggt cgtcaagagc atcagggggc gccaggcgct ctatccaggt
     6481 cttgcgttca tcccgcaggg cgtctatgca aaccagatcg tcccggcgcg tacccaggcg
     6541 accggggaac cccagtacag cggctttccc gtgacagtcg aacccgtcgc tccgggcgaa
     6601 cggcgcaaga gcacgctcga actcgtccag agcgccgtag ggatgtggaa aggtgatcag
     6661 taatggcaaa aaagattgag aatgtcggat gcccgttctg cggcgtctgc tgcgacgacc
     6721 ttgtcgtcac cgtctctgat gacggaaaga agatccttga ggttgagaac gcctgcatca
     6781 tcggcagcac ggtcttccac cgtgccatgg aagaccggca cgctctgccc cgcctccgcc
     6841 agccggacgg caccttcaaa gagatctcct acgacgaggc gatcgactac acggcaaagg
     6901 tcctgcacaa cgcaaagaaa cccctgatct acgggttcgg gtcgaccaac tgcgaaggca
     6961 tggccgccgc cgccaaggtc gccgagaagg caggagccgt gctcgacaac tgcgcatcca
     7021 tctgccacgg gtcctcgttc cttgccatat tcgataacgg ctacccgagc tgcacgctcg
     7081 gcgagacgaa gaaccgggca gacgtcatcg tctactgggg cgcaaacccc tctcacgccc
     7141 atccccggca catgtcccgc tactcgatct tcccccgcgg gttcttcacc ggcaaaggcc
     7201 acaagggccg caagatcatc gtcatcgacc cccggcatac cgacaccgcc caggtggccg
     7261 atatccacct gcaggtgcag caggggcacg actacgacct cttcgatgcg ttccgcaccg
     7321 tcctccgggg ccacgacata cccgacgtgg ttgccggcgt caagaaggag cagatcgtcg
     7381 aggccgtcga tatcatgaag aaggcccggt tcggcaccat cttctacggc atgggtctct
     7441 gccactccga cggccggaac cacaacgtgg atatcgccat cagcctgacc cgcgacttga
     7501 acgacttcac caagtggacg atcatggcga tgcggggcca ctacaacatc accgggccgg
     7561 gcgcggtctg gtcatggcaa tacgggttcc cctactgcct ggacctgacc aaacgcgata
     7621 tcgcccacat gaaccccggc gagacgagct ccgtcgacct cgccatgcgg gacgaggtgg
     7681 acgccttcat caatataggg accgatgccg ccgctcactt cccgatcgag ccggtcaaac
     7741 acctcaggaa acacccctgg atcgccatcg acccccaccc caacatggcg accgagatat
     7801 cggatctcca cataccggtg ggaatcgtcg gcgtcgaggt tccgggcatc gtctatcgga
     7861 tggacaacgt ccccatccag tatcgcaagg tcatcgaccc gcccgagggc gtcatcagcg
     7921 acgaagaact cttcgagcgg atacacctga ggctctgcga gctggaagcg gaagatgccg
     7981 taaatgtgcc ggcaaaggcc gcgcccgagg gtgtccgcgc gggggagtga accatgggtg
     8041 aactcatcgt caagaacggg ttcgtattcg accccctgca gggcatcaag ggcgaccgga
     8101 tggacgtcgg cgtcagagac ggcaagatcg tcgagacgaa cacccttaaa aatccgaaag
     8161 agatcgacgc cgccggcatg accgtcatgg cgggcggcgt ggatatccac gcccacgtcg
     8221 tcgggccgaa ggtgaacgtc ggccggctct tccggccgga agataaactc ttcaagagcc
     8281 ccctcaagcg gggcaaccgc atggagatgg ggttctccgt cc
//
LOCUS       DAOSII010000080         8311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_8018_length_8311_cov_48.508236, whole genome shotgun sequence.
ACCESSION   DAOSII010000080 DAOSII010000000
VERSION     DAOSII010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8311
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8018_length_8311_cov_48.508236"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..726
                     /locus_tag="PLV90_06985"
     CDS             145..726
                     /locus_tag="PLV90_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2121 domain-containing protein"
                     /protein_id="HOF96897.1"
                     /translation="MTLVMAYIGRQGAVMAGDMREIAFQGDDPCIEALERELYSGSIA
                     SDNDLLERAGEIGVAIRVRDDKVKVTEQDGVIVGEVTETAGSTIRQKRLYVTAGSYVI
                     AEIIDSRLRVTQRGQAGNFVVLGNDITKQVAGQCIREAWKGGTVPEAMRVILLTMQMA
                     SGVTASVSRTFMLVHTDRAMNLAGAINRASGGE"
     gene            complement(792..1109)
                     /gene="eif1A"
                     /locus_tag="PLV90_06990"
     CDS             complement(792..1109)
                     /gene="eif1A"
                     /locus_tag="PLV90_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor eIF-1A"
                     /protein_id="HOF96898.1"
                     /translation="MTNTRGSQNSGSEEIVRVRLPKKRNRELFGRADLMLGANHIRVR
                     CEDGVTRMGRIKGKIKKRLWIREGDLLIVVPWSFQDEKCDIIYRYIRPQVDWLKKHNY
                     LSQ"
     gene            1661..1921
                     /locus_tag="PLV90_06995"
     CDS             1661..1921
                     /locus_tag="PLV90_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOF96899.1"
                     /translation="MESSKLYVGNLTYSVNEEQLKELFGRYGTVNDVRVIERKGFGFV
                     EMSSPEEAEKAKDALNNTVFEGRTLKIDEARPPRPRSDFRRY"
     gene            2240..2578
                     /locus_tag="PLV90_07000"
     CDS             2240..2578
                     /locus_tag="PLV90_07000"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOF96900.1"
                     /translation="MVIGRDDAYYYYIDSTQKALYARVLSLRKDFTVLDFGCGNGRWA
                     FWFAPRVARVIGIDLSPAMVDAARRFASEQGIQNVGFTVPGDGSQFPDDSFDIVNCRP
                     RPHLHRACDG"
     assembly_gap    2580..2679
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2768..3049
                     /locus_tag="PLV90_07005"
     CDS             2768..3049
                     /locus_tag="PLV90_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOF96901.1"
                     /translation="MAEERRSVIEEERPSVRGGHASAAQAGSVEELSASAFQSYLHGM
                     DYPGKKQDLINLARKNNAPDAVIQVLEMFNDDKTFQSAADVSQEFGRVK"
     gene            complement(3127..3211)
                     /locus_tag="PLV90_07010"
     tRNA            complement(3127..3211)
                     /locus_tag="PLV90_07010"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3173..3175),aa:Leu,seq:cag)
     gene            complement(3249..4952)
                     /locus_tag="PLV90_07015"
     CDS             complement(3249..4952)
                     /locus_tag="PLV90_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOF96902.1"
                     /translation="MTDDEQKVASYEDLCANFKIDVPEYYNFGFDVIDAWAKKDRNKL
                     AMIWTDQKGNEKKYTFFDLMRLSNQAVNICIKYGIKKGDRVMLMLPRAPEWWIFTIAL
                     IKIGAVYCPATTMLTAKDLKYRIQAADIKMIITMAEYAERIEEIREECPTLAVRLMID
                     GTRDGWVSYPVELDYPAPCSHKLVNLPGMRRTRSSDPLLIFFTSGTTGEPKMVVHDHA
                     YPLGHLVTGQVWHDLHPNDLHLTISDTGWGKSAWGKLYGQWIIGACIFVYDIRSRFHA
                     TEILPLLERYGITTFCCPPTIYRMLILADLDKFDLADMRHCCSAGEPLNPEVIRAWQE
                     GTGRTIYEGYGQTETVLCIGTFPGMKCKPGSMGRPAPGWHIELHDDDGNPVGVGEEGR
                     IAIKLDPRPVGLFRGYLNNEEENCKVFQNGFYYTGDKACMDEDGYFWFIGRDDDVIKS
                     SGYRIGPFEVESALMEHPAVQEAAVIGSPDVIRGLVVKAFITLKPGYNPSESLIKDIQ
                     KHVKRVTAPYKYPRAIEFVESLPKTISGKIKRYELREREMKQFMDNNSHGVHSGSKCA
                     E"
     gene            5081..5635
                     /locus_tag="PLV90_07020"
     CDS             5081..5635
                     /locus_tag="PLV90_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HOF96903.1"
                     /translation="MDEKGYESLKIENIVASGVIADSIDLEKVSNKIKNCELNTKRFP
                     GAVYRIEKPKIASLIFSSGKVVLTGIRNKEDLHAGLDIIMHSLRDAGIDTYDEPQVAV
                     TNIVCSYDMGRYINLNKVVITLNLENIEYEPEQFPGLVYRIEDPKIVALLFSSGKIIL
                     TGGKNIEDIKRGLDFLEQRLETIM"
     gene            complement(5954..>8311)
                     /locus_tag="PLV90_07025"
     CDS             complement(5954..>8311)
                     /locus_tag="PLV90_07025"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOF96904.1"
                     /translation="RRKQALNPPDLLITTPESLQALFMGKRLREHLKNVRYVIIDEIH
                     ELADSKRGAQLAVALERLAAYAGEFQRIGLSATVGNPDEIGRFLCGARAFSLVEVPVA
                     SHLEIGVRFAGEDFAAQTRAVGDCLDAPGSTLVFVNTRVTAEALGHELYPRGDVEVHH
                     GSLSRDVRVEAEERFREGAVRTLIATSSMELGIDIGHIEHVVQFGSPRQVSRLVQRVG
                     RAGHRLDAISRGTVLATGFDDLLESLVIARRAKANECERIVPPAGAADVLANQVAAIA
                     VEYGEIEVSRIRDMIERSGVFAGCGPLLDTVCSQMAEHRLIRLDGTRVTRTGRARRYL
                     IDNLSMIPDERKVEIFDMVSRRTVGTLDESFVLGWMHTGAIFITKGQLWQVLDIEGGR
                     ITVEPAARARGEVPTWEGEQIPVPFAVAREVGALRRTRAFSRYSDIPAAIRYAERFIA
                     RMDGGNYPVPSDRLITLENTDEGVVCNVCGGHKANEALARTLSVLLSARYGTTVGIEV
                     GAYRFLLRLPPAVRALEVEETLAALEPAHLPGVLKLALKRTALFKWKLVQVAKKFGAI
                     DADADYERFSIHRLIDLFDETVIAAEAYRELFSGSMDVDAAREILLAIRDGRTAVTTG
                     RLSPLGSEGLSSSRDMIPPPMIDQAVIGTLMRRLDKEDVVLFCMHCRKWKSRTVVERV
                     PDKPQCPLCNARLIAALKPWDEQFIPAMRKKEKTEEERAIEVRFLRNANIVLSSGKKA
                     VTALAAKGVGPEAASRILATLSEGDAFYREILKAERNYVRTHRFW"
BASE COUNT         1569 a         2437 c         2556 g         1649 t
ORIGIN      
        1 ccgaccgctg ccggaaggcc ggtcttcaca accccttccg acagtgagag gaagcgacgg
       61 ggtgggtagc cggaggggca ccaccgggtt gatgcggggc gttcctggct tcccgcctcg
      121 tgccggggat tggaggttca ttgcatgact ctcgtgatgg catacatcgg aagacagggc
      181 gccgtgatgg cgggcgatat gcgggagatc gcattccagg gagacgaccc ctgtatagaa
      241 gcgctggagc gcgagctcta cagcggttcg atcgcgtccg acaacgacct gctggagcgg
      301 gccggtgaga tcggggtggc aatccgggtc agggatgata aggtcaaagt cacggagcag
      361 gacggggtga tcgtcggcga ggtgaccgag accgcgggat cgacgatccg ccaaaagagg
      421 ctgtacgtca ctgcagggag ctacgtcatc gccgagatca tcgactcccg gttacgcgtg
      481 acacagagag gccaggcggg caatttcgtg gtgctgggca acgatataac gaagcaggtc
      541 gccggccagt gtatccgcga ggcgtggaag ggtgggacgg ttcctgaggc catgcgggtc
      601 atcctgctca ccatgcagat ggcgtcaggc gtaacggcgt cggtgagcag aacgtttatg
      661 ctggtgcata ccgatcgtgc gatgaacctt gccggtgcga taaaccgggc ttccggggga
      721 gaataggttt cgggaggtgt tcgattccgc cggtggccgg tgaaggtgtc ccggccgtag
      781 cagagacggc gctactggct gaggtagttg tgcttcttga gccagtcaac ctggggcctg
      841 atgtaacggt agatgatgtc gcacttttca tcctggaaac tccagggaac aacaatcagg
      901 aggtcgccct cgcgtatcca gagccgcttc ttgatcttgc ccttgatccg ccccatgcgc
      961 gtgacaccgt cctcgcaacg gacgcggata tggtttgccc cgagcatcag gtcggcccgg
     1021 ccaaataact cccggtttcg ttttttcggt aatcgtaccc gtacaatctc ttcgcttccg
     1081 ctgttctgtg atcctcgtgt atttgtcagt tcatcaactc cgtgacaggt agcgataagc
     1141 tgcctatcgg ttctttcctg gaggtgtata ctaatgaccc cggccaccat taatgtatct
     1201 attccaggaa aattccttca atccgggtcc gccggggaag tgcaggcatg acgagggtgg
     1261 ttatcatatc ccatgtctcc cggtcgcccg aaccgttccg ccggggcctg cggtgaggtg
     1321 gtgagagcac tcccgtcacc tgatcgactg catggtgggc ggtgccgcgc attcacatgg
     1381 gtctgccccc ccgggataca ccggcggcgg tacggggaga tacgcccggg gttttacatg
     1441 atatctccgt ccgggcggag cggctaccgt gccggggcgc atacacccgg gcacgatccc
     1501 ccgcccctct cttgtgttct gggcaaacgc cgcccgggaa tgcacacctc ctctgccatg
     1561 tgcctcctct acccgggaaa atcgaccccg ggcgggcacc cgtgggttta tccgaagagt
     1621 agttaaggtt tgagcgtaca tgtgtgaaga ggatctttga atggaaagca gcaagctgta
     1681 cgtcggaaac ctcacctact cggtaaatga agagcagttg aaagaattgt ttggccggta
     1741 cggaacggtg aacgacgtca gagtcattga gcgcaaaggg ttcgggttcg ttgagatgtc
     1801 cagcccggaa gaggctgaga aggcaaaaga cgccctgaac aacaccgtat tcgagggccg
     1861 caccctgaag atcgacgagg cccggcctcc gagacccaga agcgacttcc ggcggtactg
     1921 aaccccgccc ggaagggtag tactctgaat atcccctttt ttttatgaat cggggttcgc
     1981 aagaacctca tctctgattg accggcgcgc atgccccccg tccgggcact gctgtcgttc
     2041 ctgcccggag ccgttcccct gaggtagcca gaaccggagc tgacttgccg gtgggaagtc
     2101 ccgaccaccc ggcagctacc tgctatatag gtccggccgg gtatatcggg ggtggtgatg
     2161 cataatcggg tccaatatac ccggggcgaa aaatcccctg gacgctctcc atggtgagca
     2221 cgccagccat ccgctgaggg tggttatagg gagagacgat gcctattatt attacatcga
     2281 ttccacgcag aaagcgctct atgcccgtgt tctttccctc agaaaagact ttacggtcct
     2341 cgacttcggg tgcgggaacg gccggtgggc attctggttc gcaccacggg tggcccgcgt
     2401 catcggaatc gacctctcgc ctgcaatggt ggatgcagcc cggcggttcg cgtcggagca
     2461 gggtatccag aacgtcggtt tcaccgttcc cggtgacggg tcacagtttc ctgacgattc
     2521 cttcgatatc gtgaactgtc ggccgcgacc tcatctgcac cgggcctgcg atgggtaacn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng aacacagcaa catttttatt
     2701 caccaagagc tatggggcag gcgtgtgtcc cgctgtgcgg gatgcagtca acaaggtggt
     2761 gagaagtatg gcagaagaga ggagaagcgt gatcgaggag gagagaccat cggtcagggg
     2821 tggacatgcc tctgcggccc aggccggatc ggtcgaggag ctgagcgcgt ctgcgttcca
     2881 gtcgtacctt cacggcatgg actaccccgg caagaaacag gacctgatca acctcgcccg
     2941 gaagaacaac gcgccggatg cggtcatcca ggttctcgag atgttcaacg atgacaagac
     3001 cttccagtcc gcagccgatg tgagccagga gtttggcagg gtcaaataac cttacccatt
     3061 tttgaacata tcaaagcgcc ttccgacctc gttagccgga cagcaccgat aaaatcggga
     3121 gaaggatgcg agaggccgga ttcgaaccgg cgaactccta cgagaatagg ccctgaacct
     3181 atcgcctttg acctggcttg gcaactctcg cgcctagtat tgtaatacct tcaacaaaat
     3241 aaagatgttt actccgcgca cttcgagccg gagtggacac cgtgggagtt gttgtccatg
     3301 aactgtttca tctccctctc gcggagttca tagcgcttga tcttgccgga gatcgtcttt
     3361 gggagcgatt ccacgaactc gatcgcgcgc ggatacttgt agggtgccgt cacccgtttg
     3421 acatgcttct ggatatcctt gatgagggac tccgaggggt tatacccggg cttgagagtg
     3481 ataaacgcct tgacgaccag gccccggatc acatccggcg atccgatgac cgccgcctcc
     3541 tggacggccg ggtgctccat aagcgcgctc tccacctcga acggcccgat ccggtagccg
     3601 gatgacttga tgacgtcgtc gtcgcggccg atgaaccaga agtagccgtc ctcatccatg
     3661 catgccttgt cgccggtgta gtagaacccg ttctggaaga ccttgcagtt ctcctcttcg
     3721 ttgttcaggt agccgcggaa gagcccgacc ggacgcgggt ccagcttgat cgcgatgcgc
     3781 ccttcctccc ccacgccgac cgggttcccg tcgtcgtcgt ggagctcgat atgccagccc
     3841 ggcgccggcc tccccatgga gccgggtttg cacttcatgc cggggaacgt cccgatgcag
     3901 agcactgtct cggtctggcc gtagccctcg tagatcgtcc ggcctgtccc ttcctgccac
     3961 gcccggatga cctcggggtt gaggggttcg ccggcgctgc agcagtggcg catatcggcg
     4021 agatcgaact tatcgaggtc ggcgaggatc agcatccggt agatggtcgg agggcagcag
     4081 aaggtcgtga taccgtatcg ttccagcagc ggcaggatct cggtggcgtg gaaccggctg
     4141 cggatgtcgt agacgaatat gcacgcgccg atgatccact ggccgtagag tttcccccac
     4201 gcgctcttgc cccagccggt atcggagatc gtcaggtgga gatcgtttgg gtgcaggtcg
     4261 tgccacacct gcccggtgac gaggtgcccc aggggatagg cgtgatcgtg gaccaccatc
     4321 ttgggttcgc cggtggtgcc ggaggtgaag aagatcagca acgggtctga ggaccgggtc
     4381 cggcgcatgc cggggaggtt caccagtttg tgcgagcagg gcgcggggta gtcgagctcg
     4441 acggggtaac tgacccatcc gtccctggtg ccgtcgatca tgagacggac cgcgagcgtg
     4501 gggcactctt cgcggatctc ctcgatcctc tccgcatact cggccatggt gatgatcatc
     4561 ttgatgtcgg ccgcctggat acggtatttc agatcttttg ccgtcagcat cgtcgttgca
     4621 gggcagtata ccgcgccgat cttgatgagt gcgatggtga agatccacca ctcgggggcg
     4681 cgggggagca tcagcatgac ccggtcgccc ttcttgatgc cgtattttat gcagatgttg
     4741 actgcctgat tggagaggcg catgaggtcg aagaaggtgt atttcttctc gtttcctttc
     4801 tggtcggtcc agatcatcgc cagcttgttc cgatccttct ttgcccatgc gtcgatcacg
     4861 tcaaagccga agttatagta ttcagggaca tctatcttga aattcgcgca gaggtcttcg
     4921 taggatgcta ctttttgctc gtcatctgtc ataccgcgat caataaagat ttgcgcgcct
     4981 atggtttaaa gaattcacga cgatgatgag cgctccctat tcagaatacc cataaagatc
     5041 atccattagt attatggtcc gaaaaacgat actcatatca atggatgaga agggatacga
     5101 gtcactgaaa atcgagaaca tcgttgcttc cggggtgatt gccgactcaa tcgacctcga
     5161 aaaagtatct aataaaataa aaaactgtga attgaatacg aagaggtttc ccggggcggt
     5221 ctaccgcatc gagaagccca agattgcgtc cctgatattc tcgtcgggga aggtggtgct
     5281 taccggtatc aggaacaagg aggatctcca cgctggcctt gatatcatca tgcattcgct
     5341 ccgggatgcc ggcatcgata cttacgatga accgcaggtc gcggtcacga acatcgtctg
     5401 ttcgtacgat atgggcaggt acatcaacct gaacaaggtg gtcatcaccc tcaacctcga
     5461 gaacatcgag tacgaacccg aacagttccc ggggcttgtc taccgtattg aggatccgaa
     5521 gatcgtcgcc cttctcttca gttccggcaa gatcatcctg accggcggaa agaatatcga
     5581 ggatatcaag agagggcttg atttcctgga gcagcggctc gaaactatca tgtgagactg
     5641 gtttttcact acattctttc tgaggggaga gaggagaccg aaagcccctc cgggtttttt
     5701 tgccgggcgc gatccggctg tcgggatctc acccgtatat gccgctggat tctccgggct
     5761 aaaagccggc tcttatgcct ccggcaaccc ggtgacgctc tcctctcgca tctgccgcca
     5821 gtctcacgcc gggcatcagg gcaagcctga tgcggtgaac ccggggtatt gctatggggg
     5881 atgaaggtca ggccccgatc ttatgcggaa gagcgcggag tatccgccgc accggcccgg
     5941 cttctgtctc ctcttaccag aacctgtgtg tcctgacgta gttacgctcc gccttcagga
     6001 tctcccggta gaaggcgtcc ccctcggaga gggttgcgag gatccgggat gcggcctccg
     6061 gcccgacgcc ctttgcggcg agggcggtca ctgccttctt gccgctcgag aggacgatat
     6121 ttgcgttccg gaggaaccgg acctcgatcg cccgctcctc ctccgtcttc tccttcttcc
     6181 tcatcgccgg gatgaactgc tcgtcccagg gtttcagcgc ggcgatgaga cgtgcgttgc
     6241 agagcgggca ctgcggctta tcaggaaccc gttcgacgac ggtccggctc ttccacttcc
     6301 ggcagtgcat gcagaagagg acgacgtcct ccttatcgag gcgccgcatc agcgtcccga
     6361 tcaccgcctg atcgatcatc ggcggcggga tcatgtcccg cgacgaggag agcccttcgc
     6421 tcccgagcgg actcaacctc cccgtggtga cggcagtccg gccgtcgcgg atcgcaagga
     6481 ggatctcccg ggccgcgtcc acgtccatcg agccgctgaa gagctcccgg taggcctcgg
     6541 ccgcgatgac ggtctcgtcg aagaggtcga tgagccggtg gatgctgaac cgctcgtagt
     6601 cggcatccgc gtcgatggcg ccgaacttct ttgccacctg cacgagcttc cacttgaaga
     6661 gcgccgtccg cttcagggcg agtttcagga ccccggggag atgtgcgggc tcgagcgccg
     6721 cgagggtctc ctccacctca agcgcgcgga cggccggcgg caggcggagg aggaaccggt
     6781 aggcgccgac ctcgatcccg acggtcgtcc cgtaccgggc cgagaggagg accgagagcg
     6841 tccgggcaag cgcttcgttc gccttgtgcc ccccgcagac gttgcagacg acgccctcat
     6901 ccgtattctc aagggtgatg aggcgatcgg aagggaccgg gtagttgccc ccgtccatcc
     6961 gggcgatgaa ccgctccgcg taccggatag ccgccgggat atcggagtag cgggagaacg
     7021 cccgggtcct ccggagcgcc ccgacctccc gggcaacggc gaacggcacc gggatctgct
     7081 cgccctccca cgtcggtacc tcgccccgcg cccgggcggc cggctcgacc gttatccgcc
     7141 ctccctcgat atcaaggacc tgccagagct gccctttcgt gatgaagatg gcgccggtgt
     7201 gcatccagcc gaggacgaac gactcgtcga gcgtcccgac cgtgcggcgc gagaccatat
     7261 cgaagatctc caccttccgc tcgtcgggga tcatcgagag gttatcgatg aggtaacgcc
     7321 gggcacgccc ggtccgggta acccgtgtcc cgtcgagccg gatcagccgg tgctccgcca
     7381 tctggctgca gacggtatca aggagggggc cgcacccggc aaagactcct gagcgctcga
     7441 tcatatcccg gatgcgggag acctcgatct cgccgtactc gaccgcgatt gccgctacct
     7501 ggtttgcaag gacgtcggcg gcgccggcgg gcgggacgat ccgctcgcac tcgttcgctt
     7561 ttgcccggcg cgcgatcacg agcgactcta ggaggtcgtc aaaccccgtg gcgaggacgg
     7621 tcccgcgcga tattgcatcg agacgatggc cggcccggcc gacccgctgc accaggcgcg
     7681 agacctgccg gggagaaccg aactggacga cgtgctcgat gtgcccgatg tcgataccga
     7741 gttccatcga cgatgtggca atcaacgtcc gaaccgcgcc ctccctgaac cgttcttcgg
     7801 cctcgaccct gacgtcccgc gagagcgacc cgtggtggac ctcgacgtcg ccgcggggat
     7861 agagctcgtg cccgagagcc tcggcggtca cccgggtgtt gacgaagacg agggtggacc
     7921 ccggggcgtc gaggcaatct ccgacggccc gcgtctgggc ggcgaaatcc tccccggcaa
     7981 accggacgcc gatctcgagg tggctcgcca ccggcacctc gacgagcgag aacgcccggg
     8041 ccccgcagag gaaccgcccg atctcatccg ggttcccgac ggttgccgag aggccgatcc
     8101 gctggaactc tcccgcgtag gcggccaggc gctcgagcgc gaccgcgagc tgggcccccc
     8161 gtttgctgtc ggcgagctcg tggatctcgt cgatgatgac gtacctgacg ttctttaagt
     8221 gttcacggag gcgtttgccc atgaagagcg cctgcaggga ctcgggcgtc gtgatcagga
     8281 ggtcgggagg attgagcgcc tgtttccgcc g
//
LOCUS       DAOSII010000081         8198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_8149_length_8198_cov_55.856073, whole genome shotgun sequence.
ACCESSION   DAOSII010000081 DAOSII010000000
VERSION     DAOSII010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8198
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8149_length_8198_cov_55.856073"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /locus_tag="PLV90_07030"
     CDS             <1..1363
                     /locus_tag="PLV90_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOF96905.1"
                     /translation="SSGNLLMANQADALYLYHGATIVQQVAWPADVRPREGQVHYLED
                     GVWDPRPFFIGQSRFSSATFDDVTVTAFVAPDCAYDVFSAAVASAEREILVNVYEFTD
                     PEMAADLIRARERGAAVTVLLEGGPVGGIPPEERAIAGILNRSGIVVLSMTTTDAAHA
                     KYRYDHAKYVVIDARTVLIGSENFKPGGYPAPGLQGNRGWGACLDDPKLAAYFREVLL
                     FDLSGGDIVPLEGTTADVRMPYAPAYTVEFTPCRAEGARVTPVLSPDTSALILRLIEG
                     AGESIAIEQAYITNETTYELNPYLAAAIDASRRGVAVRVLLDACWFNIEDDADNDEMV
                     DLINRIAAAEGLPLEARIVDLAANNLAKVHNKGVIVDGRAVLVSSINWNANSPAFNRE
                     AGVIVEHPDIAAYFARVFADDWDAAAADGTVAEGPDRLKVVAAACILAALMGLYIYRR
                     RRT"
     gene            complement(1367..1684)
                     /locus_tag="PLV90_07035"
     CDS             complement(1367..1684)
                     /locus_tag="PLV90_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOF96906.1"
                     /translation="MTEPPSCDIMRCDTLARRLLPRMRAEMVYRLVNERGISQSEASK
                     RLGISRAAISQYMSRKRGFTRQDFPGDLDVVIERWVSTIVSGEGTITICDVCRSAGLS
                     GRW"
     gene            complement(1681..1914)
                     /locus_tag="PLV90_07040"
     CDS             complement(1681..1914)
                     /locus_tag="PLV90_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOF96907.1"
                     /translation="MDDVNLPPSSRKILLLLEDGGALTHKELVRLSSLAPRTVRYALK
                     RLKDNDMIVEKFNFRDARQILYEYKNSPMVSAQ"
     gene            1985..2350
                     /locus_tag="PLV90_07045"
     CDS             1985..2350
                     /locus_tag="PLV90_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96908.1"
                     /translation="MLERQEKTALAVLICVVGIVLAVHLLFDAVGRPLVAAPYSEEVS
                     DGAFVLLEGRIEEIRKTSTGGHRILTVNGTAVFLPQDVAARLELDKNASVALYGVVQT
                     YRGEREVVVASAADIRVLK"
     gene            complement(2352..2549)
                     /locus_tag="PLV90_07050"
     CDS             complement(2352..2549)
                     /locus_tag="PLV90_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96909.1"
                     /translation="MPRVRINEGDLVSGAGVEGRRKKKIDESPEPPREEPAGKESAPV
                     KKGGRPSFGDADGADIFFTRK"
     gene            2687..4006
                     /gene="codB"
                     /locus_tag="PLV90_07055"
     CDS             2687..4006
                     /gene="codB"
                     /locus_tag="PLV90_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HOF96910.1"
                     /translation="MSGDERGRSGMEDYPLSAVPDEGRQEFWPITLVLLGFTFFSATM
                     WGGASIGVAFRFWPDLVWIILWGNAILAVYVAGLGYIGFRSGFSTVLSARFAFGDIGS
                     RWSDTLLGLTQLGWYAWGTATIAIALVRLLNLDAGCAAPLMIVFGLGFCMTAYVGCRG
                     LAALSWIAVPAMLLLIAVSIAVALRDAGSAAGPLAAGTLTVAEALTIVVGTFVSGGTQ
                     VANWTRFAGSGRVAVGSVLIAFFVGNGLMIAVGAVSAAIYGVSDIVEVLEIQGLLFAG
                     ILLLFLNIWTTQDNTIYNFSVAGCHFWRTERRRLITFGGAAAGTLLALAGMYDWLIPY
                     MVMMGVLIPPLGGVIMADFFVGHRGRYPPLDGAAVPRFNWRGLAAYAAGSLAALVVPG
                     IPPVNGIAAAFLIYACLIRFTAWRTRKRPVPSRDIASDAPARPNFRR"
     gene            complement(3901..5559)
                     /locus_tag="PLV90_07060"
     CDS             complement(3901..5559)
                     /locus_tag="PLV90_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOF96911.1"
                     /translation="MLEDQRWQPVPGTIGAEIYPFFRKPDVTCSNAYVIRTSGDILII
                     DTGADAGQMDRLMLLIEEALRKAPRPVTLFITHCHIDHCYQAIRDRRFRSIPGFTVAA
                     EEAGARVLESGDTIRTAAELMGWEIEPLPVNLPLLSARDCETLEVGGGITLRRQAIPL
                     CSGDPLVVYHTPGHSPDSVCIRFGGLLFIGDLLFSTSPGVAGVAGWDQPALLASVGRI
                     RWILAHEPVNFCCPGHGRCIPVQAMPALLDRLEAEAERLSGIGAFDRERLDDSLEHTA
                     DLLQEANRVFTVIAGRLDALAYRLEDLGEADEARRYLELLKSDRIDEFLEEFAQFADE
                     FRTGKKIEVQLVLKAVQVIQRIESHFAGDRLNHIVDASLLRRTDRLLTDFMNTIMGYQ
                     DTVHLVATDLPSVLRDTVAGLSTLPFSDEAFIEAADDPATYRAELVVRLAYLPLFEGV
                     AFSLDTGSPSLPPVLVDPERFSDEVVGVLGDLAAAGAVAIRLSLRQEGDAVGLLIQGS
                     DVFSARRLRFYRRKFGLAGASLAISRDGTGLFLVLQAVNRMRQA"
     gene            complement(5681..7189)
                     /locus_tag="PLV90_07065"
     CDS             complement(5681..7189)
                     /locus_tag="PLV90_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HOF96912.1"
                     /translation="MKKVAFIVLSILIFFAILAIPVDPALLAPEAKSVAAVTALMVLF
                     WVSGVIPLEATALLPLVLFPALGVLTPVQVAKSYGDEVIFLFLGGFIIAMSMQRWGLH
                     RRIALQIIRIVGASPRRLVLGFMLATAFLSMWISNTATTMMMIPIAVAIILTVMPGIT
                     RSPENPDGEEQALARCLIVSIPYAASIGGIATIIGTPPNGIFISQLAAIFPDAPAIDF
                     FTWMKFGVPFAVAFLLLAWVWLTRGPYRHMVATLPSARGIIREELTKLGPISAGERWT
                     LIVFTLTALAWIFEQTKEIGGIVIPGLDIIFPGIKDSTIAIFGALLLFVLPVDTKEGI
                     FTMDWKWAVKIPWGILLLFGGGIALSSGFLESGLAAAIVESLTLIHGLPVILLLFVVA
                     MGIVLAGEIASNTAIAAILMPIMAVTAVSMGVNPIILMMTAAVCASIGFMLPVATPPN
                     AIAFGSGYINAKDLLRSGWVLDIIGVVLWMICLYTIVLWALGLPLDLPAWAL"
     gene            7384..>8198
                     /locus_tag="PLV90_07070"
     CDS             7384..>8198
                     /locus_tag="PLV90_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3821 domain-containing protein"
                     /protein_id="HOF96913.1"
                     /translation="MAPEWIRGRGPVVLLALCLLALCAGDAGARGPTISDIQPYDTIF
                     VDEEGLNLSQLRNATTNNPITALQRYQDDNPGKSLVKSIPVADDTDFEVQEFLVGREY
                     GTYYAFNPADGTTASVFIRKPEVFLDIVLANPYHNEPLTGLTVSPNTRIAFRVAAPDV
                     GASYHVGNVYPATIDIVLTHPGGAETTLVGGINLAGLNITSTRFYTDDPGKPGAVRIG
                     DLGKPGDYTARAVWRMPAGFDTRASDSELITFTVGNRIGIDTTPTPTPTATPTA"
BASE COUNT         1513 a         2602 c         2685 g         1398 t
ORIGIN      
        1 ctcgagcggg aacctcctga tggcgaacca ggccgacgcg ctctacctct accatggggc
       61 gaccatcgtc cagcaggtcg cctggcctgc cgacgttcgt ccccgcgaag ggcaggttca
      121 ctacctcgaa gacggcgtgt gggacccgcg cccgttcttc atcgggcagt cccgcttctc
      181 ctccgcgacg ttcgacgacg tcacggtgac ggcgttcgtc gcccccgatt gcgcgtatga
      241 cgtcttctcg gcggccgtcg catctgctga gcgcgagatc ctcgtaaacg tctacgagtt
      301 caccgatccg gagatggctg ccgacctgat ccgcgcccgc gagcggggtg cggccgtgac
      361 cgtcctcctc gagggcggac cggtcggcgg cataccgccg gaggagcggg cgatcgccgg
      421 catactcaac cggagcggca tcgtggtcct ctcgatgacg acgaccgatg cggcccacgc
      481 gaagtaccgc tacgaccacg cgaagtatgt cgtcatcgat gcgaggacgg tcctcatcgg
      541 gagcgagaac ttcaagcccg gcggctatcc ggcgccgggg ctgcagggca accgcgggtg
      601 gggggcatgc ctcgatgacc cgaaacttgc ggcctacttc cgggaggtcc tcctctttga
      661 tctctccggc ggggatatcg tcccgctcga gggcacgacg gccgacgtcc gcatgcccta
      721 cgcccccgcc tacacggtgg agttcacccc ctgccgcgcg gagggggcgc gggtgacccc
      781 ggtcctctcc cccgatacga gcgccctcat cctccggctg atcgaggggg caggggagag
      841 catcgcgatc gagcaggcct acataacgaa cgagaccacc tacgaactca acccctacct
      901 tgcggccgcg atcgacgcct cccggcgcgg ggtcgcggtg cgggtcctcc tcgatgcctg
      961 ctggttcaat atcgaggacg acgcggacaa cgatgagatg gtcgacctga tcaaccggat
     1021 agccgcggcc gaggggttgc cgctcgaggc gcggatcgtc gacctcgcgg caaacaacct
     1081 cgcaaaagtg cataacaagg gcgtcatagt cgacggccgg gcggtccttg tcagcagcat
     1141 caactggaac gccaactcgc ctgcattcaa ccgggaagcg ggcgtaatcg tcgagcaccc
     1201 ggatatcgcc gcctacttcg cccgggtctt tgcggacgat tgggacgccg cagctgccga
     1261 cgggaccgtg gcggaggggc cggatcgcct caaggtggtg gcggcggcat gtatcctcgc
     1321 cgcgctcatg gggctctaca tctaccggcg gcggcgcacc tgataatcac catcggccgg
     1381 agaggccggc cgagcggcag acgtcgcaga tggtgatcgt cccctcgccc gagacgatgg
     1441 tggagaccca ccgttcgatg acgacgtcga ggtcgccggg gaagtcctgc cgggtaaatc
     1501 cgcgtttccg gctcatgtac tgagatatcg ccgcacggct gatgccgagg cgttttgaag
     1561 cctcgctctg gctgatgccg cgctcgttca ccaggcggta gaccatctcg gcgcgcatcc
     1621 gggggaggag ccgccgggca agcgtatcgc aacgcatgat gtcgcaggag ggaggttcag
     1681 tcattgtgcc gacaccatcg gggagttttt gtactcatag aggatctggc gggcgtcgcg
     1741 gaagttgaac ttttccacga tcatatcatt gtctttgagc ctctttaagg cgtagcggac
     1801 ggtgcggggt gccagactgc tgagacggac cagctccttg tgggtcaggg cgcccccgtc
     1861 ctcgagaagc aggaggatct ttcttgagga cggaggaagg ttcacatcat ccatacagat
     1921 cagttaacgc cgttaacata tcaaagtgac ggaagttata ttcccaaaaa tccattgggt
     1981 acctatgctg gaacggcagg agaaaacggc cctcgccgtt ctcatatgcg tcgtcgggat
     2041 cgtgcttgcg gtacacctcc tctttgatgc cgtcgggcgg ccgctcgtgg cggcaccgta
     2101 ctccgaggaa gtctccgacg gtgcgttcgt cctccttgag gggaggatcg aggagatccg
     2161 gaagacctcg accggcgggc accggatcct caccgtcaac gggaccgccg tcttcctgcc
     2221 gcaggacgtg gcggccaggc tcgaactcga caaaaacgca agcgtagcgc tctacggggt
     2281 cgtgcagacc taccgcgggg agcgggaggt ggtggttgcc tccgccgccg atatacgggt
     2341 tctgaagtga cttactttct cgtgaagaat atgtcggcgc cgtccgcatc accaaagctg
     2401 gggcgccccc ccttcttcac gggcgccgac tcctttcccg cgggctcctc ccggggaggt
     2461 tccgggctct catcaatctt cttcttccgg cgcccctcca cgccggcccc ggagaccagg
     2521 tctccctcat tgatcctcac tcgcggcata caggagagat ggccggcctg gtatatgact
     2581 cctctggatc gaccgggcgc cggatacccg gaagaagaac caaaacgcat tagtcctgcg
     2641 cggcagacac tccagcatcg gcgaaatggc ggtgaaccgg gagaagatga gcggcgacga
     2701 aaggggcagg agcggcatgg aggattaccc gctctccgcc gttccggacg agggtcggca
     2761 ggaattctgg ccgatcaccc tggtcctgct cgggttcacc ttcttctccg ccacgatgtg
     2821 gggcggcgcg agtatcgggg ttgcgttccg gttctggccc gaccttgtct ggatcatcct
     2881 ctggggaaac gcgatcctcg ccgtctacgt cgccggtctc ggttacatcg gcttcaggag
     2941 cggtttttcc accgtcctct ccgcccggtt tgccttcggg gatatcggga gccggtggtc
     3001 cgataccctc ctcggcctca cccagctcgg ttggtatgcc tgggggaccg cgacgatcgc
     3061 gatcgccctc gtacgccttc tcaacctcga tgcgggctgc gcggcgcccc tgatgatcgt
     3121 cttcggtctc ggtttctgca tgacagcata cgtcggctgt cgcggtcttg cagccctctc
     3181 gtggatcgcc gtcccggcca tgctcctcct catcgctgta agcatcgcgg ttgctctccg
     3241 tgatgcgggg agcgcggccg ggcccctcgc ggcagggacg ctcaccgttg ccgaagcgct
     3301 caccatcgtc gtcggaacct ttgtctccgg tggtacgcag gtcgccaact ggacacggtt
     3361 tgccgggtcc ggccgggttg ccgtcggctc cgtcctcatc gccttcttcg tcggtaacgg
     3421 gctgatgatc gccgtcggcg ccgtcagcgc cgccatctac ggcgtctccg atatcgtcga
     3481 ggttctggaa atccagggtc tcctctttgc cggtatactg ctgctcttcc tgaacatctg
     3541 gacgacccag gataacacca tctacaactt ctcggtcgcc gggtgccact tctggcgaac
     3601 cgagcgacgg cggttgatca cgtttggcgg cgccgccgcc ggaaccctcc tcgccctggc
     3661 cgggatgtat gactggctga tcccctacat ggtcatgatg ggggtgctca tccctccgct
     3721 cggcggggtg atcatggccg atttcttcgt cggccatcgg ggccgctacc ccccgctcga
     3781 cggggcggcc gttccccggt tcaactggcg ggggcttgcc gcctacgccg ccgggtcgct
     3841 tgccgcgctc gtcgtccccg gcatcccgcc ggtgaacggg atcgccgcgg cgttcctcat
     3901 ctacgcctgc ctcatccggt tcaccgcctg gaggacgagg aagaggccgg tgccgtcccg
     3961 cgatatcgcg agcgacgccc cggcaagacc gaacttccgc cggtagaacc ggagcctcct
     4021 cgcggagaag acgtcggaac cctggatcag gagcccgacg gcatcgccct cctgccggag
     4081 ggagaggcgg atcgccacgg cgccggcagc cgccagatcg ccgagcacgc cgacgacctc
     4141 gtcggagaac cgctccgggt cgaccagaac aggagggagg gaggggctgc cggtatcaag
     4201 agagaaggcg acgccctcaa agagcgggag gtaggcgagc cgcacgacca gctccgcgcg
     4261 gtatgtcgcg ggatcgtcgg ccgcctcgat gaacgcctcg tccgagaagg gcagggttga
     4321 taacccggcg accgtgtcgc ggagcaccga cggaaggtcc gtggcgacaa ggtgaaccgt
     4381 gtcctggtag cccatgatcg tgttcatgaa gtcggtgagg aggcggtcgg tcctgcggag
     4441 gagcgatgca tcgacgatgt ggttgagccg gtcgcccgcg aagtgagatt cgatccgctg
     4501 gatgacctgg acggccttga ggacgagctg gacctcgatc ttctttccgg tccggaactc
     4561 gtcggcgaac tgcgcgaact cctccagaaa ctcgtcgatc cggtcggatt tgagcagttc
     4621 caggtagcga cgggcctcgt cggcctcgcc gaggtcttcg aggcggtaag cgagggcgtc
     4681 gaggcgcccc gcgatcaccg taaagacccg gttggcctcc tggagcagat cggcggtgtg
     4741 ctccagggag tcgtcgagac gctcccggtc gaaggcgccg ataccggaga gtctctccgc
     4801 ctccgcttcg agccggtcga ggagcgccgg catcgcctga accgggatac atctgccgtg
     4861 accgggacag cagaagttca ccggctcgtg agccaggatc caccggatcc ttcccacgga
     4921 tgcgagcagc gccggctgat cccacccggc aaccccggcg accccggggc ttgtggagaa
     4981 gaggaggtcg ccgatgaaga ggagaccgcc gaaccggatg catacgctgt cgggcgagtg
     5041 cccgggggtg tgataaacca caaggggatc cccggagcag agggggatcg cctgccggcg
     5101 aagggtgatc ccgccgccca cctcaagggt ctcgcagtcg cgggcggaaa gcagcgggag
     5161 attgaccggg agcggctcga tctcccaccc catgagttcc gcagccgtcc ggatagtatc
     5221 gcccgactca agcacccggg ctcccgcctc ctctgcagcg acggtgaagc ccgggatgga
     5281 acggaaccgt cggtcccgga tggcctggta gcagtggtcg atatggcaat gggtgatgaa
     5341 gagggtgacc ggccgcgggg cttttcggag ggcctcctcg atgagcagca tgagccgatc
     5401 catctggccg gcgtcggccc ccgtatcgat gatcaggata tcgccggatg tccggatgac
     5461 gtaggcgttc gagcaggtga cgtccggttt ccggaagaag ggatagatct ctgcaccgat
     5521 ggttccgggc accggctgcc atcgctgatc ttcaagcatt ctttgcgcct cgatagggaa
     5581 gagtcgtgga gagtgttgcg ccgcagagga gaaaagatcg gcgggttctt cccggggaaa
     5641 aggtggccgg gagaaccgcc atgaaaaaag gtgccgggga tcagagcgcc caggcgggga
     5701 ggtcgagggg aagcccgagc gcccagagga cgatcgtgta gaggcagatc atccagagca
     5761 cgacgccgat gatgtcgagc acccagcctg accggagaag gtcttttgcg ttgatgtaac
     5821 cgctcccgaa ggcgatggcg ttcgggggtg tggcgaccgg gagcatgaac ccgatcgagg
     5881 cgcagaccgc cgcggtcatc atcaggatga tcgggttgac gcccatgctg acggccgtca
     5941 ccgccatgat cggcataagg atggcggcta tcgcggtgtt cgaggcgatc tccccggcca
     6001 gcacaatccc catcgccacg acgaagagga gaaggattac cgggaggccg tggatgaggg
     6061 tgagagactc gacgatagcc gcggcaagcc cgctctcgag gaatccgctt gagagggcga
     6121 taccgccgcc aaagaggagg aggatgcccc aggggatctt caccgcccac ttccagtcca
     6181 tcgtgaagat gccctccttc gtgtcgaccg ggaggacgaa gagaaggagc gcaccgaata
     6241 tcgcgatggt cgagtctttg atgccgggga agatgatatc gagaccgggg atgacgatac
     6301 cgccgatctc cttggtctgc tcgaatatcc aggcaagagc cgtcaacgtg aagacgatca
     6361 gcgtccagcg ctcgcccgcc gatatgggcc cgagttttgt gagctcctcc cggatgatcc
     6421 cccgggcgct cgggagggtc gccaccatgt ggcggtaggg gccccgggtg agccagaccc
     6481 aggcaaggag gaggaaggcc acagcgaacg gtacgccgaa cttcatccag gtgaagaagt
     6541 cgatggcggg cgcatcggga aagatcgccg caagctggga tatgaagatc ccgttcgggg
     6601 gcgtgccgat gatcgtggcg atgcctccga tgctcgcggc gtaggggatg gatacgatca
     6661 ggcagcgggc gagcgcctgt tcttcgccgt cagggttctc gggggaccgg gtgatcccgg
     6721 gcatgaccgt caggatgatc gcaaccgcaa tcgggatcat catcatcgtg gtggcggtat
     6781 tcgatatcca catcgagagg aaggcggtgg cgagcatgaa cccgagcacc aggcgcctcg
     6841 gactcgcccc cacaatccgg atgatctgca gggcgatccg ccggtgcagg ccccaccgct
     6901 gcatcgacat ggcgatgatg aacccgccga ggaagaggaa gatcacctcg tcgccatagg
     6961 acttcgccac ctgcaccggg gtgaggacgc cgagcgccgg gaagagaacc agcgggagga
     7021 gggcggtcgc ctccagggga atgacgcctg aaacccagaa gaggaccatg agggcggtca
     7081 ccgccgcaac ggactttgcc tcaggtgcaa gaagagcagg atcgacaggg atcgccagaa
     7141 ttgcaaagaa tatgaggatt gataggacga tgaaggcgac ctttttcatc ctaaaacctg
     7201 tgaggaagaa ttatttaagc atgcggaatt gagatcgcac gggccggagc gacagggagc
     7261 gagaacggat cgcctcatcc cggcagatac cgccgggccg cgctcaccgc acccgtatac
     7321 cccgggggcg ggcgggcctg acccgcacca acctatattt gcaaactcaa cacaaggaat
     7381 attatggcac cagaatggat ccgcggacga ggtcctgtcg tcctcctcgc cctctgcctc
     7441 cttgccctct gtgccggaga tgcaggggcc cgcggcccga cgatcagcga tatccagccc
     7501 tacgacacga tcttcgtaga tgaggagggg ctcaacctct cgcagctcag gaacgcgacc
     7561 accaacaacc ccatcacggc gctccagagg taccaggacg acaaccccgg aaaatcgctc
     7621 gtaaagagca tcccggtcgc cgacgacacc gactttgagg tgcaggagtt cctcgtgggc
     7681 cgtgagtacg ggacctacta cgccttcaac cccgccgacg gcacgacggc atccgtcttc
     7741 atccgcaaac cggaggtctt cctcgacatc gtacttgcaa acccctacca caacgaaccg
     7801 ctgaccggcc tcacggtatc cccgaacacc cggatagcgt tccgggtggc cgcccccgac
     7861 gtcggggcat cctaccatgt gggcaacgtc tacccggcga cgatcgatat cgtgctcacc
     7921 cacccgggcg gcgcggagac caccctcgtc ggcgggatca accttgccgg attgaacatc
     7981 acctcgaccc ggttctacac cgacgatccg ggtaaacccg gtgcggtccg gatcggcgac
     8041 ctcggtaagc cgggggacta caccgcccgg gccgtctgga ggatgccggc cggtttcgat
     8101 actcgggctt ccgactccga actcataacg ttcacggtcg ggaatcggat cggtatcgat
     8161 acgaccccca cgccgacgcc gaccgcgacg ccgaccgc
//
LOCUS       DAOSII010000082         8108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_8263_length_8108_cov_50.800820, whole genome shotgun sequence.
ACCESSION   DAOSII010000082 DAOSII010000000
VERSION     DAOSII010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8108
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8263_length_8108_cov_50.800820"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="PLV90_07075"
     CDS             complement(<1..787)
                     /locus_tag="PLV90_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOF96914.1"
                     /translation="MTPPLQELLSDTLAGHRLTEEEAVRLFATRDRDVWAIAAAADEL
                     REERVGDVVTYIRNQNINVTNLCVNACGFCGFSRKPGDPDVYRYDETVVRKKAREARE
                     RNVTEICTVSGLHPGFDAQSYIDIYSWIRDEAPAVHIHASNPMEVAYAARRSGLSTRE
                     VLIAMKAAGLSTLCGTAAEVLVDSVRRVICPDKIDTATWARVIREAHYLGIPSTATIM
                     YGHCESDADRARHLGILREIQDETHGFTEFVPLSFIHENTALYR"
     gene            903..1220
                     /locus_tag="PLV90_07080"
     CDS             903..1220
                     /locus_tag="PLV90_07080"
                     /inference="COORDINATES: protein motif:HMM:NF020406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1805 domain-containing protein"
                     /protein_id="HOF96915.1"
                     /translation="MAQQILCHQRTRLSYKDAYGYVIPVGGTSLMTWVTDEGLIATAG
                     FDIEALANAGVPAAIVGPSEEESTLENLDDLMDAEVKAMNLPAIQRRIEVGMSGREAL
                     NRI"
     gene            complement(1230..2318)
                     /gene="cofH"
                     /locus_tag="PLV90_07085"
     CDS             complement(1230..2318)
                     /gene="cofH"
                     /locus_tag="PLV90_07085"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HOF96916.1"
                     /translation="MPHSLHTLLCDTLAGHRLTEEEAVQLFTTRGRDIWKIAAAADEL
                     REERAGDAVTYVRNQNIHVTNICKNLCGFCGFGRRADDPGAFYDDPATVREKARTARE
                     RNVTEICLLSGVHPDYTLDSYADLISSVREAAPGVDIHTASPDEIVWAAAQSGTSTVA
                     AIERLREAGLGTLQGTAAEILVDSVRRIICPRKCDTATWVRVIREAHHLGIRSTATIM
                     YGHCESDADRARHLGILREIQDETHGFTEFIPLSFIHENTALYRAGLARAGATGREDI
                     LMVAVARLFLDNFDHVQVSWGKVGTKMAEAALLAGADDLGGTMFCDDVSTDAGSEGAD
                     YLDPRDMQRIAQDIGRTLRQRTTTYGAV"
     gene            complement(2348..3016)
                     /locus_tag="PLV90_07090"
     CDS             complement(2348..3016)
                     /locus_tag="PLV90_07090"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOF96917.1"
                     /translation="MSDVTLLQRVASILAAFRESDNRVVSLARDLLWVVVVVGGIALA
                     LYLFAGTWPAVVTIESESMVPHMNVGDLVLVVDEDRFGGLTTWVEGQQTGHSSFGDYG
                     DVIVYRPNGADSVHPIIHRAMTYVDTAAVEESGLAQFYADPHGGYITKGDNNPYIDQG
                     NLRIAGIGLIEPVRKEWIVGKALVAVPLVGYLPLHLAEFAVIVVVIILIHDLILARRK
                     GKEE"
     gene            3119..4522
                     /locus_tag="PLV90_07095"
     CDS             3119..4522
                     /locus_tag="PLV90_07095"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase II small subunit"
                     /protein_id="HOF96918.1"
                     /translation="MLAEAEIVRRFLALKHQVHPDVVSYIREQNDPALIDRIAAGVPD
                     GTLVISAEHIPGLRKVRDGIRFLVDPELEVITGSAGSTGSVTGHEDAVHYFRDRYNNL
                     SSMIRGRMTTIPIEALVKSPHRYRDEECGIIGMVADARTTAKGHRLVEVEDPTGAIRV
                     LFNKGRDDSFLEAERILPDEVIGVKGKLSGDGGLLFAEHLFRPDIPINHAPFKSDRPG
                     KAVLISDVHVGSDTFLEEGWNRFADWLQDSDAAYLLIAGDLVDGIGIYPGQENELTIR
                     NIYEQYDVFAGMLRDLPSRIRIIAAPGNHDAVRMAEPQPAIPAEFSGKFPANCTLVEN
                     PCLLNLQGIRVLMYHGRSFDDMIGLIPGASYERAGEIMDEMLKRRLLASPYGMRTPIA
                     AMKSDRLVIDPLPEILLTGHVHICGVTRYRGVLGVNAGTWQSQTSFQKQMNIHPTPAR
                     AFVVDLQTLQPEVMDFS"
     gene            4613..6637
                     /locus_tag="PLV90_07100"
     CDS             4613..6637
                     /locus_tag="PLV90_07100"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOF96919.1"
                     /translation="MDLVYLAPIGAVLALLFAGYSYMSIKREGTGTELMQKIAAAIHE
                     GAMVYLNKQYRAIALFVIVLAIVLAIWIEPLTAACFVLGAALSATAGYIGMFTATAAN
                     VRTTNAARRGIADAFRVSFSSGSVMGMAVVGLGLLGLSIAYMVISTFTGLTQAEVLTS
                     VTGFGLGASSIALFARVGGGIFTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGDN
                     VGDVAGMGADLYESYVGSIIAAMVLGVAVAATQFPNANIMNVIILPLLIAAVGIIASI
                     IGSFFVRTNKTESSAIHMAFNKGLLSALVLVVIATYFLVTQILGAEYIGVFYATVAGL
                     IAGFAIGLITEYYTSFDRKPTLSIVKSTETGAATTIITGFAKGMESTIWPVLIVAVAI
                     YISYQVAGLYGIAIAAVGMLSTLGISLSVDAYGPVADNAGGIAEMSHQKPEVREITDT
                     LDAVGNTTAAIGKGFAIGSAALTALALFAAYTQAVNLSIIDVSVPNVFIGLLIGAMLP
                     FLFCSITMMAVGKAAYSIVHEVRRQFKEIAGLMEGTVEPDYTSCIAISTHSALREMIV
                     PGILAVVAPLAVGFVLGPEALGGLLVGSLASGFLLAVTMANSGGAWDNAKKYIEQGHL
                     GGKGSPAHKAAVVGDTVGDPFKDTSGPSLNILLKLMSMVALVFAPLILVV"
     gene            6744..7685
                     /locus_tag="PLV90_07105"
     CDS             6744..7685
                     /locus_tag="PLV90_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOF96920.1"
                     /translation="MFRIYRTVEGATVPRTDETGRFEPGAWVSLVNPTADEIREAAEK
                     LSIPGDYIRAALDEEERPRTESEDGVTLIIIDIPMPYPEQTILYTTMPLGIIVTPDVI
                     VTVCLRENAIIEDFANGRVKLFYTAKKTRFVLQILFRNASYFLQYLRQIERISDRLGA
                     ELNQSMRNRELIQLMNLKKSLVYFSTSLKSNQIVLDKTLTFQPLRMYADDTDLLEDVI
                     IENKQAIEMANTYSTILSETMDAFASIISNNFNNVLKLLTSITIILAIPTMIASFLGM
                     NVPVPLQDEPYGFLIIIALSLIVSSLLAVAINRKGWL"
BASE COUNT         1528 a         2601 c         2437 g         1542 t
ORIGIN      
        1 cccggtagag agcagtattc tcgtggatga acgagagagg gacgaactcg gtgaacccat
       61 gggtctcgtc ctggatctca cggaggatgc cgaggtgccg ggcgcggtcc gcatcgctct
      121 cgcagtgacc gtacatgatc gtcgcggtcg atgggatacc aagataatgc gcctcccgga
      181 tgacccgggc ccacgtcgcc gtatcgatct tgtcagggca gatcactctc cggacactgt
      241 ccaccaggac ctcggccgcc gtcccgcaga gtgtcgagag gccggccgct ttcatcgcga
      301 tgagcacctc ccgggtggag agaccgctcc tcctcgcggc gtatgccacc tccatcgggt
      361 tgctcgcgtg gatgtggaca gcgggcgctt cgtcccgtat ccaggagtag atatcgatgt
      421 aggattgggc gtcaaacccc gggtggaggc cgctcaccgt gcagatctcg gtcacgttcc
      481 gctcgcgcgc ctcccgggcc ttctttcgca cgacggtctc gtcgtagcga tatacgtcgg
      541 gatcgcctgg ttttcgagag aacccgcaga acccgcaggc gttcacgcag aggttggtca
      601 cgttgatgtt ctggttccgg atgtaggtga cgacgtcacc tactctctct tcccggagtt
      661 catcggcggc agcggcgatc gcccagacat cccggtccct ggtcgcgaag aggcggacgg
      721 cctcctcctc cgtgagccgg tggccggcaa gcgtatcgct gagcagctct tgcagcggtg
      781 gcgtcataca cctcgaccat ctttacagga tgatcccatt tggcgcggcc agttcaagaa
      841 ccttcggatt cgctcccggc ggagggcata tacttatctg tcccggggac atctccgcag
      901 ccatggctca gcagatccta tgtcaccagc ggacacggct cagctacaag gacgcatacg
      961 gctacgtcat cccggtcggc gggaccagcc tgatgacctg ggtgaccgat gaagggctga
     1021 tcgcgaccgc cgggttcgat atcgaggcgc tggcaaatgc cggagttccc gcggcgatcg
     1081 tgggcccctc ggaggaagaa tccaccctgg agaatctcga cgacctgatg gacgccgagg
     1141 tgaaggcgat gaaccttccg gcgatacagc gccgcatcga ggtcggtatg tcggggcgcg
     1201 aggccctgaa caggatttga ggggtcgcct cacaccgccc cgtaagtcgt cgtccgctgc
     1261 cggagcgtcc ggccgatgtc ttgggcgatc cgctgcatat cgcgcgggtc caggtagtcg
     1321 gcaccctcgc tgccggcgtc ggtcgagacg tcgtcgcaga acatcgtccc cccgaggtcg
     1381 tcggcgccgg caagaagcgc cgcttccgcc atcttcgttc ccaccttccc ccaggagacc
     1441 tggacgtggt cgaagttgtc gaggaagagg cgggcgacag cgaccatcag gatatcctct
     1501 ctccccgtcg cccccgctcg cgcgagaccc gcacggtaga gagcggtatt ctcgtggatg
     1561 aacgagaggg ggatgaactc ggtgaaccca tgggtctcgt cctggatctc gcggaggatg
     1621 ccgagatgcc gggcgcggtc cgcatcgctc tcgcagtggc cgtacatgat cgtcgcggtc
     1681 gatcggatcc cgagatggtg cgcctcccgg atgacccgga cccatgtcgc cgtatcgcat
     1741 ttcctcgggc agatgattcg ccggacgctg tccaccagga tctcggccgc cgtcccctgc
     1801 agggttccaa gacccgcttc ccggagccgc tcgatggccg cgacggtcga ggtgccgctc
     1861 tgtgccgccg cccagactat ctcgtccggg cttgcggtgt ggatatccac gccgggggcg
     1921 gcctcccgca cggaggagat gaggtcggca taagagtcga gcgtgtagtc ggggtgcacc
     1981 cccgagagga ggcagatctc ggtgacgttc ctctcacgcg ccgtccgggc cttctcccgc
     2041 acggtcgcgg ggtcgtcata aaatgcgccc ggatcatcag cccgccgccc gaatccgcag
     2101 aacccgcaga ggttcttgca tatgttggtg acgtggatgt tctggtttcg gacgtaggtg
     2161 acggcgtcgc ctgccctctc ctcccggagc tcgtcggcgg cggcggcgat cttccagata
     2221 tcccggccgc gggttgtgaa cagctgcacc gcctcctcct cggtgaggcg gtggccggcg
     2281 agcgtatcgc aaagcagggt atgcagtgag tgcggcatcg taaacaactc tattgatctc
     2341 ccgggactta ttcctctttc cctttgcgcc gcgcaaggat gagatcgtgt atgaggatga
     2401 tgacgacgac gatgacggca aactccgcga ggtggagggg gaggtagccc acgagcggca
     2461 ctgcaacgag ggcttttccg acgatccact ccttcctgac cggctcgatc aggccgatgc
     2521 ctgctatgcg gaggttcccc tgatcgatat aggggttgtt gtcccctttg gtgatgtagc
     2581 cgccgtgcgg gtcggcgtag aactgtgcaa gccccgactc ctccacggcg gcggtatcga
     2641 cgtaggtcat cgcccggtga atgatcggat ggacggagtc ggcgccgttc gggcggtaga
     2701 cgatcacgtc gccgtaatcc ccaaacgacg agtgcccggt ttgctgcccc tcgacccagg
     2761 tcgtgagccc gccgaaccgg tcctcatcga cgacgaggac caggtcgccg acgttcatgt
     2821 gcgggaccat gctctccgac tcgatcgtga ccacggcagg ccaggtgccg gcaaagaggt
     2881 agagggcgag ggcgataccg cccacgacca cgacgaccca gaggaggtcg cgggcgaggg
     2941 agacgacccg gttatcgctc tcccggaacg ccgcgaggat cgatgctaca cgctgcagca
     3001 gggtgacatc cgacatttgt tgtaaagaga gtgcactccc ttcatataca taggttcccc
     3061 ggggaccggg cggagaagag ataaacccat attataccgg ggagaataac atgagtccat
     3121 gctcgccgaa gcggagatcg tacgccggtt ccttgcgctg aaacaccagg tccatcccga
     3181 cgtggtgagt tacatccggg aacagaacga tccggcgctc atcgaccgga tagcggcggg
     3241 ggttcccgac ggcaccctgg tcatatccgc ggagcatata ccggggctca ggaaggtcag
     3301 ggatgggatc cggttcctcg tcgatccgga actcgaggtg atcacgggga gcgccgggtc
     3361 caccgggagc gtcaccggcc acgaggacgc cgtccactac ttccgggacc gctacaacaa
     3421 cctctcctcg atgatccgtg gcaggatgac gacgataccc atcgaggcgc tggtgaaatc
     3481 cccccaccgt taccgggacg aggagtgcgg catcatcggt atggtggccg acgcccggac
     3541 gaccgcaaaa gggcaccggc tcgtcgaggt ggaggacccc accggggcca tacgggtgct
     3601 cttcaacaag ggacgggacg actcctttct ggaggcggag cggatcctcc ccgacgaggt
     3661 gatcggggta aaggggaaac tctcgggcga cggggggctt ctctttgccg aacacctctt
     3721 tcgcccggat atccccatca accacgcccc gttcaagagc gataggccgg ggaaagccgt
     3781 cctgatatcc gacgtgcacg tcgggagcga cacattcctc gaggaagggt ggaaccggtt
     3841 tgccgactgg ctccaggact cggacgccgc atacctcctg atagcgggcg atctcgtcga
     3901 cggtatcggc atctaccccg gacaggagaa cgaactgacg atccggaaca tatacgagca
     3961 gtacgacgtc tttgccggca tgctccgcga cctcccttcg cggatacgga tcattgccgc
     4021 tcccgggaac cacgacgccg tcaggatggc cgaaccccag cccgcgatac cggcggagtt
     4081 ctcggggaaa tttccggcaa actgcactct cgtcgagaac ccctgcctct tgaacctcca
     4141 gggtatccgg gtcctcatgt atcatggccg gtcgtttgac gatatgatcg ggctcatacc
     4201 gggcgcaagt tacgagcggg ccggggagat catggatgag atgctcaaac gccggctgct
     4261 tgcctccccc tacggcatgc ggacccccat cgccgcaatg aagagcgatc ggctcgtcat
     4321 cgacccgctc cccgagatcc tcctcacggg gcacgtccac atctgcggcg tcacccgcta
     4381 ccggggcgtc ctcggggtga acgccggcac ctggcagtcg cagacatcgt ttcagaaaca
     4441 gatgaatatc catcctacac ccgcgcgggc gttcgtcgtc gatctccaga cacttcagcc
     4501 tgaagtgatg gatttcagct gatattcacc ctttttcccc ggatgttttc cacatattta
     4561 aaaatgttga attcctctgt atatggtact ctgttggagg aatgagagag agatggatct
     4621 ggtgtatctg gcaccgatag gtgccgttct cgctctcctg ttcgcaggat actcgtatat
     4681 gagtatcaaa cgggagggca cgggtaccga actgatgcag aagattgctg ccgccattca
     4741 tgaaggagca atggtctacc tgaacaaaca gtatcgcgct attgcactat tcgtcatcgt
     4801 gcttgccatc gtccttgcga tctggataga accgctcacc gcagcatgtt tcgtgctcgg
     4861 cgcggcgctc tcggcgacgg ccggctatat cggcatgttc accgccacgg ctgcaaacgt
     4921 ccggaccacg aacgccgcgc ggcgagggat cgcggatgcg ttccgggtct cgttttcgag
     4981 cgggtcggtc atgggcatgg ctgtcgtcgg gctcgggctt ctcgggctct cgatcgcgta
     5041 tatggttatc agcacgttca caggccttac ccaggccgaa gtcctcacct cggtgaccgg
     5101 gttcggtctc ggggcaagtt cgatcgccct cttcgcccgt gtcggcggcg gtatattcac
     5161 caaggccgca gacgtcggtg ccgatctggt cggcaaggtc gaagcgggca tccccgagga
     5221 tgacccccgc aatcctgccg tcatcgccga caacgtcggc gacaacgtcg gcgacgtcgc
     5281 cggcatgggc gcggacctct acgaatccta cgtcggctcg atcatcgcgg cgatggtcct
     5341 cggcgttgcc gtcgcggcca cgcagttccc gaacgccaat atcatgaacg tgatcatcct
     5401 cccgctgctc atcgccgcgg tcggtatcat cgcatccatc atcggttcgt tcttcgtccg
     5461 aaccaacaag accgagagca gcgccatcca catggccttc aacaaagggt tgctctccgc
     5521 gctcgtcctg gtcgtcatcg ccacttattt cctggtgacc cagatcctcg gggcggagta
     5581 catcggcgtc ttctacgcca ccgtcgccgg tctcatcgca gggttcgcta tcggtctgat
     5641 caccgagtac tatacctcgt ttgaccggaa accgaccctt tcaatcgtga agtccaccga
     5701 gaccggggcc gcgaccacca tcatcaccgg gtttgcaaag gggatggaga gcaccatctg
     5761 gccggtcctg atcgtcgccg tcgcgatcta catatcctac caggtcgccg ggctctacgg
     5821 tatcgccatc gccgccgtcg gcatgctctc gacgctcggg atctccctct ccgtcgacgc
     5881 ctacggcccg gtcgccgaca acgccggcgg tatcgccgag atgtcgcacc agaaacccga
     5941 agtccgcgag atcaccgata cgcttgacgc cgtcggcaac accaccgccg ctatcggcaa
     6001 ggggttcgcc atcggctctg cggctctgac ggcgctcgcc ctcttcgccg cctacaccca
     6061 ggcggtcaac ctctcgatca tcgacgtctc ggttccgaac gtcttcatcg gcctcctgat
     6121 cggtgcgatg ctccccttcc tcttctgctc catcacgatg atggcggtgg ggaaggccgc
     6181 gtacagcatc gtccacgagg tccgccgcca gttcaaggag atcgccggcc tcatggaggg
     6241 gacggtcgag cccgactaca cctcctgcat cgccatatcc acccactcgg cgctgcgcga
     6301 gatgatcgtg cccggcatcc tcgccgtcgt cgcaccgctc gcggtcgggt tcgttctcgg
     6361 gcctgaagcg ctcggcggcc tgctcgtcgg ttcgcttgca tccgggttcc tcctcgccgt
     6421 cacgatggcg aactccggcg gggcctggga caacgcgaag aagtacatcg aacagggcca
     6481 cctcggcggg aagggatcgc cggcccacaa ggcagcggtc gtcggcgaca ccgtgggcga
     6541 ccccttcaag gatacgtcgg gcccgtccct gaacattctc ttgaagctga tgtctatggt
     6601 cgcgctggtc tttgcaccgc tgatcctggt cgtctaactc accttttttt aaggcccgcc
     6661 gggagcggag ccaggacata ccttcatccc gttctacgga taaccagata ctgcagatac
     6721 cctggagcgg gggtgaagac cgcatgttcc ggatctaccg tacggttgag ggggccacgg
     6781 tgccgcggac ggacgagacc gggcggtttg aaccgggcgc ctgggtctct ctcgtcaacc
     6841 ccaccgcgga tgagatcaga gaggccgcag agaaactttc catcccgggc gattatatcc
     6901 gggcggcgct cgacgaggag gaacgcccgc gaaccgagag cgaagacggc gtcaccctga
     6961 tcatcatcga tatcccgatg ccctacccgg agcagacgat cctctacacg acgatgcccc
     7021 tcgggatcat cgtcaccccc gacgtcatcg tcaccgtctg cctccgcgag aatgcgatca
     7081 tcgaagattt cgcaaacggc cgggtgaagc tcttctacac cgcgaaaaaa acccggttcg
     7141 tgctccagat cctctttcgg aatgcatctt acttcctcca gtacctgcgc cagatcgagc
     7201 ggatatcgga tcggctcggg gccgagctga accagtcgat gcggaaccgg gagctcatcc
     7261 agctgatgaa cctcaaaaag agcctggtct acttctcgac atcgctcaag agcaaccaga
     7321 tcgtcctcga caagaccctg acgtttcaac cgctccggat gtacgcggac gacaccgatc
     7381 tcctcgagga cgtcatcatc gagaacaagc aggcgattga gatggcgaac acctactcga
     7441 cgatcctctc ggagactatg gatgcgttcg cctcgatcat atcgaacaac ttcaataacg
     7501 ttctaaaact cctcacgtcg ataaccatca tcctcgccat cccgacgatg atcgcaagtt
     7561 tcctcggcat gaacgtcccg gtccccctgc aggacgagcc ctacggattt ctcatcatca
     7621 tcgccctctc tctgatcgtc tcctccctcc ttgcggtggc gatcaaccgg aaaggatggt
     7681 tgtaggccga tcgtccgggc ccgcgccggg tgccggccat ccgcggcacc cggcgcaagc
     7741 atatcctcat cgcggccttg caagaagcac cggcaggtgc atgatctcaa ccccgtcgcc
     7801 cgcctgaccg caaagtttat cccggaatta tacattattg atcatgataa atccacatga
     7861 tagatcatgt aaaatagggg gttgcccgtg atggggaatc acccgccggg gccggcaccc
     7921 gttgcccatc cccggcatgt atcgaccacg gatcaggtgc cgaaagaccg gcagacctaa
     7981 cggatcgacc ggcactgcac ggtaatgtga cccgcgcggg tcccggccgt gcaccccggc
     8041 atgacagaag ataggaatga ggatatatga cagagacatt tgctgtcaaa ctggaggaga
     8101 agtgctac
//
LOCUS       DAOSII010000083         8107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_8264_length_8107_cov_40.366617, whole genome shotgun sequence.
ACCESSION   DAOSII010000083 DAOSII010000000
VERSION     DAOSII010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8107
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8264_length_8107_cov_40.366617"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PLV90_07110"
     CDS             <1..651
                     /locus_tag="PLV90_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOF96921.1"
                     /translation="ACTVFLRGCPARCFYCHNVALQGGEDLRPVDEVVAMIRSSRPVA
                     GAVVFSGGEPTLQGPALVRLAAAARGMGLSVGLHTNGIFPGVIEALLRERLVDMIALD
                     IKTTWERYDDLIELTAADAVKRSLALCKEAKADGSLGTCEIVTTLFRGREGDLPTIAE
                     AARGLDLVLQQGVTAGYVPLSRQEIETAAAPLGQKVRIRTREDGEVGYDPEQNTSP"
     gene            777..1325
                     /locus_tag="PLV90_07115"
     CDS             777..1325
                     /locus_tag="PLV90_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOF96922.1"
                     /translation="MKVIGIVGMPASGKGEVSRIARDLGIPVVVMGDAIREKVKEAGL
                     PLTDANLGAISGKLRADLGMDAIARITIPVIEATAAPVVLVDGIRGDYEVTAFREHFP
                     DFTLIAIVSSFETRYARLANRGRSDDSLTAGELRSRDERELGWGLLRALAQADCRITN
                     EGTLEEFAAEARSLLCRVGGLS"
     gene            1322..2095
                     /locus_tag="PLV90_07120"
     CDS             1322..2095
                     /locus_tag="PLV90_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOF96923.1"
                     /translation="MSLTPYFSSSSKVWESRDWIFGLEEIGYVGWEIVADGKYRLDNP
                     ENFIAIQENLASTGLRSTVHAPYSDLNLASLNYPIWRESIRQTCCCIRHAADLTDRVT
                     IHPGFLSPVGKLVPEKVWEMQKTALVEIGKHAEDHGVLACVENMISIKDFLCRYPEEI
                     LGLTEGIAGIGVTLDIGHANTNGLVDAFLAHVGEVNHLHIHDNHGQSDEHLALGDGTI
                     NWEKVGRIVARDYSGPVVIEGRTLEEAGRSLAVFRKWFV"
     gene            2083..3036
                     /locus_tag="PLV90_07125"
     CDS             2083..3036
                     /locus_tag="PLV90_07125"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOF96924.1"
                     /translation="MVRIAGETLHVHFLGTAGSLPSPQRNPPCILIRRGSDTLLFDCG
                     EGAQQQMMRAKTGFTIDAIFVTHWHADHFLGIFGIVETLGFMERTAPLPIYGPPWVVE
                     FVDLIQRINRHTRGFAVTAHPLDHGSVVPFNGYTVRAFATIHGIPGLGYVLEEDERPG
                     RFNRDHAIALGVPPGPLFGRLQRGETVRIVRDGVETEVRPGDVLGEPRPGRKVVYTGD
                     TRPIQNQPGVAALIRGADLLIHDATFDDQESSRAREVLHATAGEAGEAAAALNARMLA
                     LVHISSRYASTANHIRDAKRQYEGDVILPADLTVLEIPFRS"
     gene            3058..>3765
                     /locus_tag="PLV90_07130"
     CDS             3058..>3765
                     /locus_tag="PLV90_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOF96925.1"
                     /translation="MEQGLCSLSRRRLILTGLGALLVSAAVSSVAGIYLGAAREVLML
                     IPGLMVLLPSIIDMRGNIAGVLASRLSSSMHLGVFSIDFEGGSVLGDNIRASFVITVL
                     IAFVLGIFAYLASLLSGLPTVGVTDLVLISVTSGIVSGLVVMGITVVIALASYRYGLD
                     LDMIAAPTVTTSGDIVTLPILMLSAVFVINLPPWARLTLGIAALAAAGAAILYTRHRP
                     VGIGTIVRENLALLIPLS"
     assembly_gap    3766..3865
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3866..4415
                     /locus_tag="PLV90_07135"
     CDS             <3866..4415
                     /locus_tag="PLV90_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOF96926.1"
                     /translation="VGIGTIVRENLALLIPLSILGTLAGLTYSLNLDDLVTFAALLIL
                     IPPFMGGLGSIGGILGSRLSTGMHTGELAPSLVPGHDVVHHFIISYLYTLILLPLLAL
                     IAHGAAVLMGLNSPGLGILVTISLIAGLIVMTLVNGAAYVTASLSFRYGLDPDDFGIP
                     VITTLIDLIGAATLVSVINLLL"
     gene            4449..5651
                     /locus_tag="PLV90_07140"
     CDS             4449..5651
                     /locus_tag="PLV90_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HOF96927.1"
                     /translation="MMEREYQPISFKDVLVEMKDISELMVDLSYSAILFDSKEIALEV
                     INLEESMNKLVYQARIQSVLGARRLEEAEAMSGMLQVAEAAERIANSASDVAKLILKN
                     VRFPAKLKRIFPEAEELVTRVIVSQSSELAGCTLGGLKVQSTTGMQVIAIRRGKGWIY
                     GPDKTTRVEVGDILISRGPEAGADILSLMAGTVERPRVEPSPAGEVDDIDRAVGLIIE
                     MKNLSELSVGLAYTSLLFNNREVAHEVVALDSTLDDMRYDLDLWILEAARKIDDVHKL
                     RGLLYLSAFAQAISDSAHSIVDVLLRDIEIPPVFKKIVRESDEIITRMPVAASSPLVG
                     RTLKEASLGTVTGMVVLAIKHGDRWVYRPGKDVRLENGDIIIAKGRRDGECRLHELAG
                     YAVGMPEE"
     gene            5666..7501
                     /locus_tag="PLV90_07145"
     CDS             5666..7501
                     /locus_tag="PLV90_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOF96928.1"
                     /translation="MTDTEDTIVYRLGANCDLDEVEEGKTYLGRVQGFAPFGVFVQLN
                     DRVKGLVHKSNVKVQHSERDPIIVHVLQIRSNGNIDLEEVTPTVYQTRNVTKKTTTVL
                     LADIGKKIGRTVLVEAEVAQVKQTSGPTIFTIVDESGTANAAAFIEAGVRAYPEVELG
                     DIVGVMGEVMQRNNQLQIEVGSMTVLEPEDAARVRERIDAALDLRAEPADLPFLVESD
                     VLSALRPQMRLVAKEIRKAVLTARPIILRHHADADGICAAVAVEQAVTALIRETSGDF
                     DAEYFLFKRSPSKAPFYEIEDITRDIDFALKDNIRYGQKMPMILLMDNGSTEEDMPSL
                     KITRIYGLPVLVVDHHHPDAIVDDYLIGHVNPYHVGGDYGITAGMLGTEIARLINPAV
                     EDQIRHLPAIAGAGDRSEAPERARYLALAAPDYSADDCRDIALALDYEQFWLRFSDGR
                     EIVKDILNLGGNPERHEKFVDLLVEEANRAIEEQMGAIIPHVERRMLPNGAHLFMLDV
                     EIFAHRFTFPPPGKTSGEVHDRLVREHPGEPVVTLGFGPDFAVLRSRGVMMNIPRMVR
                     ELHTEIAGGGVSGGGHLVVGSIKFVEGMRDVVIESLIRKIGEAPI"
     gene            7768..>8107
                     /locus_tag="PLV90_07150"
     CDS             7768..>8107
                     /locus_tag="PLV90_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96929.1"
                     /translation="MAGNRWMWRGFLAVLLVAAVALVPSVAARNVAPATPVTWTLPPT
                     PAETPTPEETPIVVETPTPEETRIAVETLTPEETLAAIQGGETPAETLTPEEETLAGI
                     TSTTANDTTGV"
BASE COUNT         1516 a         2458 c         2539 g         1494 t
ORIGIN      
        1 gcctgcacgg tcttcctccg ggggtgcccc gcccgctgct tctactgcca taacgtcgcc
       61 ctccagggcg gagaggacct tcgccccgta gatgaggtcg tcgcgatgat ccggagctcc
      121 cgcccggttg ccggcgccgt cgtcttctcc ggcggagaac cgaccctgca ggggccggcg
      181 ctcgtccgcc ttgccgccgc cgcccgcggc atggggctct ctgtcggcct gcacacaaac
      241 ggcatctttc caggagtgat cgaggccctc ctccgcgaac gcctggtgga catgatcgcg
      301 ctcgacatca agaccacgtg ggagcgttac gacgacctta tcgagcttac cgccgctgat
      361 gccgtgaaac ggtcgctcgc cctctgtaaa gaggcaaaag ccgacggcag cctcgggacc
      421 tgtgagatcg tgaccaccct cttccgtggc cgcgaaggcg acctcccgac gattgcagag
      481 gccgcccgcg ggctcgacct cgtcctccag cagggggtga ccgcagggta cgtgcccctc
      541 tcccggcagg agatcgagac ggcggcggca cctctcgggc aaaaggtccg gatacggaca
      601 agggaggacg gcgaggtcgg atacgacccg gagcagaaca cctccccgtg acgttaagca
      661 tattatcgtc gggaacgcaa ggttcatgga tatggttgtg gcggccgcca cgagccagga
      721 gtccggagca ccggcacggt tgttctcgat agagcacgat acaacggaga aacctgatga
      781 aggtcatagg aatcgtcggt atgccggcaa gtggaaaagg agaggtatcg aggatcgccc
      841 gggacctcgg gataccggtc gtggtcatgg gcgacgcgat acgggagaag gtgaaggagg
      901 ccgggcttcc cctgacggac gcaaacctcg gcgcgatctc ggggaagttg cgcgcagacc
      961 tcggtatgga tgctatcgcc cggatcacca tcccggtcat cgaggcaacg gccgctccgg
     1021 tggtcctcgt ggatggcatc aggggagact acgaggtgac ggcgtttcgg gagcacttcc
     1081 ccgacttcac gctcatcgcg atcgtctcgt cgtttgagac gcgctacgcg aggcttgcga
     1141 accgggggcg gtcggacgac tccctcaccg ccggagaact ccggtcccgc gacgagcggg
     1201 agctcgggtg ggggcttctg cgggctctcg cgcaggcgga ctgccggatc acgaacgaag
     1261 gcacgctcga agagtttgct gcagaggccc ggagcctgct ctgccgcgtg ggagggctct
     1321 catgagcctt accccgtact tctcctcctc atcgaaggtc tgggagtcgc gcgactggat
     1381 cttcggcctc gaagagatcg ggtacgtcgg gtgggagatc gtcgccgacg ggaagtaccg
     1441 cctggacaac cccgagaact ttatcgctat ccaggagaac ctcgcgagca ccggccttcg
     1501 ctcgaccgtt cacgctccct acagcgactt gaacctcgcc tcgttgaact accccatctg
     1561 gcgcgagtcc atccgccaga cctgctgctg catccgccac gcggccgacc tgaccgaccg
     1621 ggtgacgatc cacccgggtt tcctctcccc tgtgggaaaa ctcgtccccg agaaggtctg
     1681 ggagatgcag aagaccgcac tcgtcgagat cgggaagcat gcggaagatc acggcgtgct
     1741 tgcatgcgtc gagaacatga tcagcatcaa ggacttcctc tgccgctacc ccgaggagat
     1801 tctcggactc acggagggga tagcggggat aggggtcacg ctcgatatcg ggcacgcgaa
     1861 caccaacggc cttgtcgatg cattcctcgc gcatgtcggg gaggtcaatc acctccacat
     1921 ccacgacaac cacgggcagt cagacgagca cctggcactt ggtgatggaa ccatcaactg
     1981 ggagaaggtc gggcgcatcg tcgcccgcga ctactccggc ccggtcgtga tcgaggggcg
     2041 aacgcttgaa gaggcgggac ggagccttgc cgtcttcagg aagtggttcg tatagccggc
     2101 gagaccctgc acgttcactt cctcggcacg gccggatcgc ttccgtcacc gcagaggaac
     2161 cccccctgca tactgatccg gcggggctcc gacaccctgc tcttcgactg cggggagggc
     2221 gctcagcagc agatgatgcg tgcgaagacc gggttcacca tcgatgccat cttcgtcacg
     2281 cactggcacg ccgatcactt cctcggcatc ttcgggatcg tcgagacgct cgggttcatg
     2341 gagcgcaccg ccccgctccc tatctacggc ccgccctggg tcgtggagtt cgtcgacctc
     2401 atccagcgga tcaaccgcca cacccggggg ttcgccgtca cggctcatcc cctcgatcat
     2461 gggtcggtcg tgccctttaa cggctacacc gtcagggcgt ttgcgacgat ccacggcatc
     2521 cccggtctcg gttacgttct ggaggaggac gagcggccgg ggaggttcaa ccgtgaccac
     2581 gcgatcgcgc tcggcgtccc gccgggcccg ctcttcggtc ggctccagcg cggggagacg
     2641 gttcgcatcg tccgcgacgg cgtcgagacc gaggttcggc ccggcgacgt gctgggcgag
     2701 ccgcgtccgg ggaggaaggt cgtctacacg ggagatacgc ggcccatcca gaatcaaccc
     2761 ggggtcgccg cgctgatccg gggcgccgac ctcctgatcc acgacgccac gttcgacgac
     2821 caggagagca gccgcgcacg agaagtcctg catgccaccg ccggcgaggc cggcgaggca
     2881 gcggcggcct taaacgctcg catgcttgcc ctggtgcaca tcagctcccg ctatgcaagc
     2941 acagcaaacc atatacgcga tgcaaagcga cagtatgaag gagacgtgat cctcccggcc
     3001 gatctcacgg tcctggagat cccttttcgg agttgacgat aatggacggt gatggtgatg
     3061 gagcagggtc tatgcagcct gagccgccgc cgtctgatcc tgaccggtct aggcgccctg
     3121 ctcgtcagcg ccgccgtatc gagcgttgcg ggcatctatc ttggcgccgc acgtgaagtc
     3181 ctcatgctga tacccggcct gatggtgctc ctgccgtcga tcatcgatat gcggggcaac
     3241 atcgccgggg ttctcgcatc ccgcctctcg tcgtcgatgc acctcggggt cttctccatc
     3301 gactttgagg gggggtccgt cctcggtgac aacattcgcg cttcgtttgt cataaccgtc
     3361 ctcatagcct tcgttctcgg catattcgcc tacctggcaa gccttttgtc cggcctcccg
     3421 acggtgggcg tcaccgatct tgtgctgatc tcggtcacga gcgggatcgt ctcggggctc
     3481 gtggtgatgg ggatcaccgt ggtcatcgcg cttgcgagtt accgctacgg cctcgacctc
     3541 gacatgatcg ccgccccgac cgtcaccaca tccggggata tcgtcacgct cccgatcctc
     3601 atgctctccg cggtcttcgt catcaacctc ccaccctggg cgcgcctgac gctcgggatt
     3661 gccgcgctcg ccgcggccgg tgccgccatc ctctacaccc ggcaccggcc ggtgggaatc
     3721 gggacgatcg tcagagagaa cctcgctctc ctcataccgc tcagcnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnggtgg gaatcgggac gatcgtcaga gagaacctcg
     3901 ctctcctcat accgctcagc atccttggga cactggccgg gttgacttac tccctcaacc
     3961 tcgacgatct ggtgacgttt gccgcgctcc tcatcctgat accgccgttt atgggaggcc
     4021 tcggctcgat cggcggcata ctcgggtcgc gcctctcgac cggaatgcac accggcgaac
     4081 ttgccccctc gctcgtcccg gggcacgacg tcgtgcatca ttttattatt tcctacctct
     4141 atacgttgat actgcttcca ctgctggcgc tcatagcgca tggcgcggcg gtgctcatgg
     4201 ggttaaacag ccccggcctt ggaatactgg tcactatcag ccttatcgcg ggtctgatcg
     4261 tcatgaccct ggtgaatggt gcggcatacg tcactgcgag cctctcattc cggtacggcc
     4321 tcgatcccga tgatttcggt atccctgtca taacaaccct gatcgacctg atcggtgcgg
     4381 ccacgcttgt atcagtgata aacctgctgt tgtaacgaaa ggatatgaaa agaacggttg
     4441 agaaacccat gatggaacgt gaataccagc cgatcagttt caaagatgtc ctggtcgaaa
     4501 tgaaggatat ctctgaactg atggttgatc tctcgtactc tgcgatactc tttgacagca
     4561 aagagatagc cctcgaagtg atcaatcttg aggagagcat gaacaaactg gtctatcagg
     4621 ccaggatcca gagtgttctc ggcgcaagac gccttgaaga ggcagaggcg atgagcggca
     4681 tgctccaggt ggccgaggca gcggagagga ttgcaaactc ggcgtccgat gtagcaaaac
     4741 tcatactgaa gaacgtccgg ttcccggcaa aactcaagcg catctttccc gaggccgagg
     4801 agctcgtcac ccgggtgatc gtcagccaga gcagtgaact cgccggctgc acactcggcg
     4861 gcctcaaagt ccagagcacg acgggcatgc aggtgatcgc catccggcgc ggaaaaggct
     4921 ggatctacgg tcctgataag acaacccggg tggaggtcgg ggacatcctg atatcccggg
     4981 gtccggaggc cggggcggat atcctctccc tgatggcagg gacggtggag cgtccccggg
     5041 tcgaaccctc gccggcgggc gaagtcgacg atatcgaccg ggcggtcggg ctgatcatcg
     5101 agatgaagaa cctctcggag ctctctgtag ggctcgcgta cacctcgctg ctcttcaaca
     5161 acagggaggt ggcccacgag gtggttgccc tcgattcgac gctcgatgat atgcgctacg
     5221 acctggacct ctggatcctt gaggccgcac gaaagatcga tgatgtacat aaactccgtg
     5281 gcctcctgta cctctccgca tttgcccagg cgatcagcga ctccgcccac tccatcgtcg
     5341 atgtcctcct gcgcgacatc gagatcccgc cggtcttcaa gaagatcgtc cgggaatcgg
     5401 acgagatcat cacgcggatg cctgttgcgg catcgtcgcc gctcgtgggg aggactctca
     5461 aggaggcgtc gctcgggacg gtgaccggga tggtcgtgct cgccatcaag cacggcgacc
     5521 ggtgggtcta tcgcccggga aaggatgtcc gactggaaaa cggtgatatt atcattgcca
     5581 agggcagacg tgacggtgag tgccggctgc atgaacttgc cgggtacgcc gttggaatgc
     5641 ccgaagaatg atcaggtgaa ctaccatgac agatacagaa gatacgattg tctatcgcct
     5701 cggcgcgaac tgcgatctcg acgaggtgga ggaaggaaag acctatcttg gacgggtgca
     5761 gggttttgcc ccgttcgggg tcttcgtcca gttaaacgac cgggtcaaag gactcgtcca
     5821 taagagcaac gtgaaggtgc agcacagcga gcgcgacccg atcatcgttc acgtcctcca
     5881 gatccggagc aacggcaaca tcgaccttga ggaggtcacg ccgacggtct accagacccg
     5941 gaacgtgacc aagaagacga cgaccgtgct tcttgccgat atcgggaaga agatcggcag
     6001 gacggtgctg gtcgaggccg aggttgccca ggtcaagcag acgtcggggc cgacgatctt
     6061 cacgatcgtg gacgagtccg gcaccgcgaa cgcggccgcg ttcatcgagg cgggtgtccg
     6121 tgcctatcct gaggtcgaac tcggcgatat cgtcggcgtc atgggcgagg tgatgcagcg
     6181 caacaaccag ctccagatcg aggtcggatc catgacagtc ctggagccgg aggatgcggc
     6241 gcgggtcagg gagcggatag atgcggcgct tgatctccgt gcggaaccgg ccgatctccc
     6301 gttcctcgtg gagagcgatg tcctttcggc gctcaggccg cagatgcgtc ttgtggcaaa
     6361 ggagatccgg aaggcggtcc tgaccgcgcg gccgatcatt ctccggcatc acgcggatgc
     6421 ggatgggata tgcgctgccg tcgcggtcga gcaggcggtg accgcgctga tccgggagac
     6481 gagcggagac ttcgatgcgg agtacttcct cttcaagcgg tctccatcca aggcgccgtt
     6541 ctatgagatt gaggatatca cgcgggatat cgacttcgcc ctcaaggata acatccgcta
     6601 cgggcagaag atgccgatga tcctcctgat ggacaacggg tcgaccgagg aggatatgcc
     6661 gtcgctgaag atcacccgga tctacggtct tccggtgctg gtcgtggacc accaccatcc
     6721 tgacgcgatc gtggacgact acctgatcgg gcacgtcaac ccctaccacg tgggcgggga
     6781 ttacggcatc acggccggga tgctggggac agagatcgcc cgcctgatca atccggcggt
     6841 cgaggatcag atccggcacc tgccggcgat cgcaggggcc ggcgaccgga gcgaggcgcc
     6901 ggagcgtgcc cggtaccttg ctcttgccgc gcctgattac tcggcggacg actgtcgtga
     6961 tatcgcgctc gcgctcgatt acgagcagtt ctggctcagg ttcagcgatg ggagggagat
     7021 cgtcaaggat atcctgaacc tcggcgggaa cccggagcgc cacgagaagt tcgtcgacct
     7081 gcttgtcgag gaggcgaaca gagcgatcga ggagcagatg ggggccatca taccccatgt
     7141 cgagagacgg atgttgccga acggtgccca tctcttcatg ctcgatgtgg agatcttcgc
     7201 tcaccggttc acgttcccgc cgccgggcaa gacgtcgggc gaggtccatg accggctggt
     7261 ccgggagcac ccgggagaac cggtcgtcac gctcgggttc gggccggact ttgccgtcct
     7321 gcgttcccgg ggcgtcatga tgaatatacc ccggatggtg cgcgaactcc acaccgagat
     7381 tgccggcggg ggagtgagcg ggggcggtca ccttgtcgtc gggagcatca agttcgtgga
     7441 gggcatgcgc gacgtggtga tcgagagcct gatcaggaag attggtgaag caccaatctg
     7501 atgcaacttc tttcctgtcg gtggtgcgga tagcagtttc ttgcctgtgg caagaggatc
     7561 gcgctcgccg gcgcgggaag tgccaccgcg atgccggtca cggcccccct gccatccggt
     7621 atactgttcc tcttctcttc tccgcgtgat ctgttggccg ggagtatact ccggctgttt
     7681 atggtgcggc accggggatt tgccgccggt gtggttttta aaaacaaccg aaatataaaa
     7741 ataaagttat atcatacggg tatcctcatg gcaggaaata gatggatgtg gagagggttt
     7801 ctggctgtgc tgcttgtggc ggcggtggcc cttgtgccct ccgtggccgc ccggaatgtg
     7861 gcgcctgcga ctcctgtgac ctggaccctt cctccgaccc cggcggagac accgacaccg
     7921 gaggagactc cgatcgtggt ggagacccca acaccagagg agactcggat cgcagtggag
     7981 accctgactc ctgaggagac actggcagcg atccagggcg gtgagacacc ggcggaaact
     8041 ctgacaccgg aggaggaaac tctggcaggg ataaccagca ctacggccaa cgatacgacc
     8101 ggtgtga
//
LOCUS       DAOSII010000084         8064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_8311_length_8064_cov_45.716569, whole genome shotgun sequence.
ACCESSION   DAOSII010000084 DAOSII010000000
VERSION     DAOSII010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 8064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8064
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8311_length_8064_cov_45.716569"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(327..1583)
                     /locus_tag="PLV90_07155"
     CDS             complement(327..1583)
                     /locus_tag="PLV90_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOF96930.1"
                     /translation="MGGERMPVRGIPGQAALILILVALLVFVTGCVGAGQSGGIPDSG
                     ADTAGYRTVVDSRGVEVQVPKEIDRVVTVSSGLIEEVMFVLGEEEKTVGVGGINIQNV
                     WVYSYPLDDGKFVNGSDGRNVLQVLYPPIKELPLIMGADAAINYETLAGLDPDVVILR
                     VGTGGLTSMENEGVQKTIQTIESLRIPIVVLKGPPCFDDPDIATISDEIRIIGSVFGK
                     EERADKLADYLESQTQVVFERTKDIPDAERPTVLVFGMNAHRAGALGTQGAGSVKGTD
                     TVESYFIEKIVHAKNAYRNTGSPLISAEQLLALDPDAIVLSTSYGYHPPGELYDAMYY
                     KNVGELSAVKNHRVMSLPWTPYYSNRLEYPIDVMVIAKAAYPGRFADIDLGEWLLDFY
                     KNVYGVDDGTAKALRSAQWMDWCVEV"
     gene            complement(1559..2290)
                     /locus_tag="PLV90_07160"
     CDS             complement(1559..2290)
                     /locus_tag="PLV90_07160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOF96931.1"
                     /translation="MFLTQSRENPERIRHVIESLPLRTDSTVLDIGAGPGTLSVPLAG
                     RVARVTAVEPAAGMAEVMEEYAAEEGVSNLSIVRKRWEDIDPAVDLDGPYDLVVASYS
                     LGMPDIKTAVEAMCGVSSGWVYLFWFAGTTSWEQAMVDLWPGLHGEEFRFGPKVDVLW
                     NVLYSMGIYPNVETVRMEHTRRFSSIDTAVDEFREQYRITSPAQEAVLREYLAANLRR
                     ENGGGFLHSGTTTRVRLWWEANGCR"
     gene            complement(2422..3273)
                     /locus_tag="PLV90_07165"
     CDS             complement(2422..3273)
                     /locus_tag="PLV90_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF96932.1"
                     /translation="MLHVQDMHFRYGDFPVLSGVSFSVGKGELCGLFGPNGSGKTTLF
                     KCCLRFLRAEQGIVRMHGEDTSQCSIERLARLVAYVPQEHKPPFPYLVREVVLMGRTP
                     HLGGFFGVRRRDREIAMDALATLEISDLADRPYSQLSGGQRQMVLMARAIAQDTPVLF
                     LDEPTSALDFQNQMRIWEIMRDIAGEGKTILACSHDPNHVAWFCDRVVVVGEGVVVAE
                     GHPEDAISEVVLEEIYQNICSVRTLDGVQMVMPRCVVERNKKLKGDRPSHDVPLARAW
                     SGAETGS"
     gene            complement(3279..4358)
                     /locus_tag="PLV90_07170"
     CDS             complement(3279..4358)
                     /locus_tag="PLV90_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOF96933.1"
                     /translation="MTESTGFNHLLKKLGYETVEGFGEERKILILLSLAAILFVTASI
                     AVVLGTYGITAADVYRTIFVHLTFGDVGSIPRLHNTIIWDIRVPRIILAVSVGGALAI
                     AGAVFQGVFRNPLVEPYILGVSSGAAFGAALGIVFPAIFFSIQVSAFAFGALAVTLAY
                     VLARSRGETPIITLILAGVIIGSIFSAFVSLLKYMADDTALREIVFWLMGGFYYTTWD
                     DVRLLVPVVILSFIILWALGWKLNVLSMGDDEARALGVSPEKYKFIVIALATGITAFA
                     VSLVGIIAWVGLMMPHASRMLLGPDNRFVIPASLMMGGCYLIVCDTLARTLTTAEIPV
                     GILTSILGAPYLCYLLRSKGRVIFG"
     gene            4510..5736
                     /locus_tag="PLV90_07175"
     CDS             4510..5736
                     /locus_tag="PLV90_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOF96934.1"
                     /translation="MTCNTKTIGLALLTACLLALAFSCGCTGTTTDTGSVDSGARTVT
                     DMRGRAVELPATIERVVAIDDGFVEGLLYRFGMQDRIVGLGGTWVQDYTYPFEGAGGA
                     TYEYRNGTNPVSYLIPGLADLPVLVETGTAINYETLASLDPDVVILREGAWAFSAGSD
                     EALGKTIATIDSLGIPLVILVGPPFQESPTIGHIGEEIRLVGEVFSREKDADALASYL
                     DDQIKEIRRRTADIPENEKPRMMQLGLSPRARQGGGAGMAWGGDTIESYFIEEIANAK
                     NAYRGTGAFVVVSTEQILALDPDVIVLPTAQGYHPASELYTAPYYRNLQELTAVKNHR
                     VYALPWTPFNWAKRLEYPIEAMVIAKAAYPDRFADIQVHEWVLDFYQNVYGVDHATAE
                     ELRSAQWLDWMVEAEF"
     gene            5788..6270
                     /locus_tag="PLV90_07180"
     CDS             5788..6270
                     /locus_tag="PLV90_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2124 domain-containing protein"
                     /protein_id="HOF96935.1"
                     /translation="MELKEQLSGVPGMLRPFKAYLRGLEPGAGDQVVYYGCPGTCTPF
                     IELLGFAVRDLPVEQVYVPYVDETAAKIIRPVGGVGMQVSGDAARVDPEVVVLMGGLA
                     MPGVPVTKEAVRAVVGAHTGAKVVGICFMQMFEKAGWLDAFDFDLIIDAAIDPVRVWG
                     "
     gene            complement(6254..7681)
                     /locus_tag="PLV90_07185"
     CDS             complement(6254..7681)
                     /locus_tag="PLV90_07185"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOF96936.1"
                     /translation="MIALTKQQRVGVLITLLAISVFLTYYFHAVLMLGTVFSHFFYVP
                     IILTALWWEKRSVLVALFLGGLVVVSSLLYRPDILTLNDYGRALMFVLIALVVASLSG
                     QLKRREREVEKERDLVRHYLDVAGGLFVVINADHTIRLINRRGCELLGYCEEELLGKD
                     WFATVVPEAYQEARREIFDAAIASGTAYPDPRESPVVTRSGEELILAWQDTVLTGNDG
                     RPVGIIGSGSDITGRIRVEGELRAAHEEANLYLDIMVHDINNANAVALGYTDLLTDML
                     EGKEREMTLKLRGGVNRSIEIIQNVSTLRRLRSSETVTRPVDLDAVIRAGIAHHPDAG
                     IIYEGRPVEVMADDLLAEVFANLIGNAEKFGNPGVEVVIRVEESDGAVEVSVEDTGPG
                     VPDTVKPLLFTRFVRGTNRGSGKGLGLYITRMLISRYGGRIRVEDRVPGHPEEGAAFR
                     FTLPRPDRPWEPQPAVNPVAVTPRP"
BASE COUNT         1599 a         2492 c         2417 g         1556 t
ORIGIN      
        1 gcaggctcta ccgcctctcc gggtgcgggg tgctctgatc ggccgtcaat tcaccctgcc
       61 ggcggctgcc tccgcatcgc gagcgagcct tcccgaccgg atgggccgga agatcgcgat
      121 atcccgtcca gcacatccgc aagaataacg atcgcgggat ggctcacggc aagcgcccgg
      181 ccatcgggcc cgcagcaagg gctgtggggt cctccgtgat ctcgcaggtg gcccgtgcag
      241 ggccgggcga gcgtggggaa tcgattccgt tcagcatcct gacagcctgt accggattgg
      301 tcgtcgtccc ctcacccgca cgcaggtcac acctccacgc accagtccat ccactgcgcc
      361 gatcgcagcg ctttcgctgt cccgtcatcg acaccataga cgttcttgta gaagtcaagg
      421 agccactccc cgagatcgat atcggcaaat cgtccgggat acgctgcctt cgcgatcacc
      481 atcacgtcga tcgggtactc gaggcggtta ctgtagtagg gtgtccaggg aagggacatg
      541 acccggtggt tcttgacggc cgagagttcg ccgacgttct tgtagtacat ggcatcgtag
      601 agttctccag gcggatggta gccgtaggac gtgctgagga cgatggcgtc ggggtcgagg
      661 gcaagaagtt gttcggccga tatgaggggg cttcccgtgt tccggtaagc attctttgcg
      721 tggacaatct tctcgatgaa gtacgactcg accgtatcgg ttcccttcac gctgccagcc
      781 ccctgggttc cgagcgcccc ggccctgtga gcgttcatgc cgaatacaag gacggtcggc
      841 cgttcggcat caggaatatc ctttgttctc tcaaagacca cctgtgtctg cgattcaagg
      901 tagtcagcga gcttatcagc ccgttcctcc ttcccaaaga cggagcctat gatccggatc
      961 tcatcggaga tcgtcgcaat atcaggatca tcgaagcatg gaggaccttt gagtacgacg
     1021 atgggaatcc tgagggactc gattgtctgg atggtcttct gcaccccttc attctccatc
     1081 gaggtgagcc ctccggtacc gacgcggagg atgacgacat ccggatcgag tccggcaagg
     1141 gtctcatagt taatggccgc atctgccccc atgatcagcg ggagttcttt gatcggcggg
     1201 tagagaacct gcaggacatt gcgtccatca ctcccgttca cgaactttcc atcatcaagg
     1261 ggataggagt acacccagac gttctggatg tttatccccc caaccccgac ggtcttctcc
     1321 tcttccccga ggacgaacat cacctcctcg atgaggccgc tggagacggt gacgacacgg
     1381 tcgatctctt tcggcacctg gacctcaacc ccgcgggagt cgacaaccgt ccggtaaccg
     1441 gcggtgtcag cgcccgaatc cggtatgcct ccgctctgac cggcaccgac gcaccccgta
     1501 acaaagacca ggagagcgac caggataagg atcagggctg cctgtcccgg tatccccctc
     1561 accggcatcc gttcgcctcc caccagagcc tcacccgggt cgtcgttccc gagtgcagga
     1621 agccgccccc gttctcccgg cggaggttcg cagcgagata ctcccgaaga accgcctcct
     1681 gcgccggaga cgttatcctg tactgctccc ggaactcgtc cacggcggta tcgatacttg
     1741 agaacctgcg ggtatgttcc atccgcaccg tctccacgtt cgggtagatg cccatcgagt
     1801 agaggacgtt ccagaggacg tcgacttttg ggccgaaccg gaactcttcc ccgtgcagcc
     1861 ccggccagag gtcgaccatc gcctgctccc aggacgtcgt cccggcaaac cagaagaggt
     1921 agacccatcc cgatgacaca ccgcacatcg cctcgactgc ggttttgata tccggcattc
     1981 cgagggagta ggaagcgacg acgagatcgt aggggccgtc gagatctacc gccgggtcga
     2041 tatcctccca gcgcttccgc acgatcgaga ggtttgatac gccctcttcg gctgcgtact
     2101 cttccatgac ctccgccata cccgccgccg gctcgacggc ggtcacgcgg gcaacgcggc
     2161 cggccagggg gaccgaaagc gttcccggcc cggcaccgat atccagcacc gtcgagtcgg
     2221 tcctgagcgg gaggctctcg atcacgtggc ggattcgttc ggggttctcg cgcgactggg
     2281 tgagaaacat tcgagccttc tcccgggagg cccataccga ggcgcatccc ccgttccccc
     2341 gagtttccgt atttttttcg tagaggtctt tccagacgcc attccagtca atctctctat
     2401 acatcgttgt tcatctccgt ttcacgatcc ggtttccgcc ccactccatg ccctggccag
     2461 agggacgtca tgagaagggc ggtcaccttt cagctttttg ttccgctcca ccacgcacct
     2521 gggcatcacc atctggacgc catcgagcgt ccggacggag cagatgttct ggtaaatctc
     2581 ttcaagcacc acttcggata tcgcgtcctc ggggtgacct tcggccacga cgaccccctc
     2641 tcccacgacc acgacccggt cacagaacca cgccacgtgg ttcgggtcat ggctgcaggc
     2701 gaggattgtc tttccttctc cggcaatatc gcgcatgatc tcccatatcc gcatctggtt
     2761 ctggaagtca agcgcactcg tgggttcatc gaggaagaga acgggagtat cctgcgcgat
     2821 cgcgcgggcc atgagcacca tctgccgctg gcccccggag agctggctgt agggcctatc
     2881 cgcgaggtcg ctgatctcga gggtggcaag tgcatccatc gcaatctccc ggtcgcgcct
     2941 ccggacgccg aagaatccgc cgagatgcgg agtcctgccc atgaggacga cctcgcggac
     3001 aaggtaggga aacggtggtt tgtgctcctg cggcacgtaa gcgacgagtc gcgcaagacg
     3061 ttcgatgctg cattgcgagg tatcttcacc gtgcatccgg acaataccct gttctgcgcg
     3121 gaggaaccgc aggcagcatt tgaagagcgt tgtttttccc gagccgttcg gaccgaagag
     3181 gccgcagagt tcccctttcc cgaccgagaa ggagacgccc gagagtacag ggaaatctcc
     3241 gtatcgaaaa tgcatatcct ggacgtggag catggcagtc acccaaagat caccctgccc
     3301 ttactgcgga gcaggtagca gagatatggc gcaccgagga tggaggtcag gatcccgacc
     3361 gggatctcgg ccgtcgtcag cgtccgggcg agagtgtcgc agacgatcag gtagcatccg
     3421 cccatcatca gggacgccgg aatgacgaag cgattgtcgg gcccaaggag catcctcgat
     3481 gcgtggggca tcatgagccc gacccacgcg atgatcccga cgagcgagac ggcgaatgcg
     3541 gttatgccgg tggcaagcgc gatgacgatg aacttgtact tctcagggct gaccccgagt
     3601 gcccgcgcct cgtcgtcgcc catcgagagg acgttgagct tccacccgag cgcccagagg
     3661 atgatgaaac tgagaatgac cacggggacg aggagtctga cgtcatccca ggtcgtgtag
     3721 tagaatcccc ccatcagcca gaagacgatc tcgcgcaacg ccgtgtcgtc ggccatgtac
     3781 ttgaggagcg agacgaatgc cgagaagatc gaacctatga tgactccggc gaggatgagg
     3841 gtgatgatag gcgtctcacc cctgcttcgg gcgaggacgt aagcaagggt gacggcgagt
     3901 gcaccgaacg cgaatgcaga gacctggatc gagaaaaata tagccggaaa gacgattccg
     3961 agcgccgcac cgaacgcggc ccccgacgag acaccgagga tgtaaggctc gacgaggggg
     4021 tttcggaaga ccccctggaa gacggcaccc gcgatggcaa gcgccccgcc gaccgagacg
     4081 gcgaggatga tccggggcac gcggatgtcc catatgatgg tgttgtgcag cctcggaata
     4141 cttccaacat cgccgaacgt gagatggaca aagatcgtcc ggtagacatc cgcggcggtg
     4201 atgccgtacg tgcccaggac gaccgcaata gaggcagtta cgaagaggat agcagcaagg
     4261 gagaggagga tgagaatctt tctctcttcg ccgaacccct cgacagtctc gtaacccagt
     4321 tttttgagga ggtggttgaa gcctgtggac tcggtcatgc gatctccacc cacacgggcg
     4381 gtgcgggtac accctctctc tgtattaacc attatataaa ttgtagcaat taatacgact
     4441 ttgacatata gttctcttca ttgatgtgaa atgcgatcgt ttttatagca tttatcccaa
     4501 ctatccccta tgacttgtaa tacaaaaacc atagggctcg ccctgcttac cgcctgcctc
     4561 ctcgccctgg ccttctcctg cggctgcacg ggaacaacaa cggatacggg atctgtagat
     4621 tccggggccc ggacagtaac ggatatgcgc ggcagagcgg ttgaactgcc ggcaaccatc
     4681 gaacgggtcg tcgccatcga cgatggtttt gtcgaagggc tcctttaccg gttcggcatg
     4741 caggaccgga tcgtcggcct cggtggcaca tgggttcagg attacaccta ccccttcgag
     4801 ggagccggcg gagcgacgta tgagtacagg aacggcacga acccggtcag ctatcttatc
     4861 cccgggcttg ccgatctccc ggtgcttgtg gagaccggaa cggcgatcaa ctatgagacc
     4921 cttgcttctc tcgacccgga tgtcgtcatt ctccgcgagg gggcatgggc gttcagcgcc
     4981 ggatccgatg aggctctcgg aaagacgatc gccacgatag attcacttgg tatccccctc
     5041 gtcatccttg tcggcccgcc cttccaggag agcccgacca tcggtcatat cggtgaggag
     5101 atcagactcg tcggcgaggt cttctcgcgc gagaaggatg ccgatgcact tgcatcctac
     5161 ctggacgacc agatcaaaga gattcggaga cgcacggcag atatccccga aaatgagaaa
     5221 ccccgtatga tgcagctcgg tctctcccca cgcgcccggc agggcggcgg tgccgggatg
     5281 gcctggggcg gcgatacgat cgagtcttac ttcatcgaag agattgcaaa cgcaaagaac
     5341 gcctacaggg ggaccggagc gttcgtcgtc gtatcgaccg agcagatcct cgcgctcgac
     5401 ccggatgtga tcgtcctccc gaccgcccag ggctaccacc cggcaagcga gctctacacc
     5461 gcaccttact accggaacct gcaggaactc acggccgtga agaaccacag ggtctacgcc
     5521 ctcccctgga cacccttcaa ctgggcgaag cgccttgaat acccgatcga ggcgatggtg
     5581 atcgcaaagg ctgcctatcc agaccggttt gcggatattc aggtgcatga atgggtgctg
     5641 gacttctacc agaacgtcta cggggtcgac catgcgacgg cagaggagct ccggtccgcc
     5701 cagtggctgg actggatggt cgaggcagag ttctgaaacc ctgccccatc cggaaccggt
     5761 atatgccccg gcgataacca gaccttcatg gaactcaaag aacaactctc cggcgtcccc
     5821 ggtatgctcc ggccgttcaa ggcctacctc cgcgggctgg agcccggtgc gggcgaccag
     5881 gtcgtttatt acggctgccc cggcacctgc accccgttca tcgaactcct ggggtttgcc
     5941 gtccgcgacc tccccgtcga gcaggtctat gtgccctacg tggatgagac ggcggcaaag
     6001 attatccgcc cggtcggggg cgtcggcatg caggtctccg gtgacgcagc ccgcgtcgac
     6061 ccagaagtgg tcgtcctgat gggcgggctt gccatgccgg gcgtcccggt cacaaaagag
     6121 gcggtgcggg cggtcgtcgg cgcccatacc ggcgcaaagg tcgtgggtat ctgcttcatg
     6181 cagatgtttg agaaagcggg ctggctcgac gcctttgact ttgacctgat catcgatgcc
     6241 gccatcgatc cggtcagggt ctgggggtga ccgccaccgg gtttacggcc ggctgcggct
     6301 cccacggccg gtcggggcgg ggcagggtga accggaacgc cgccccttcc tccgggtggc
     6361 ccggcacccg gtcctcgacc cggatccggc cgccgtagcg tgagatgagc atcctcgtga
     6421 tgtagagccc gagccctttc ccgctccccc tgttcgtgcc ccgcacaaag cgggtgaaga
     6481 ggagaggttt caccgtatcc ggcacgccgg gtcccgtatc ctcgaccgag acctcgaccg
     6541 ccccgtcact ctcctcgacc cggatgacga cctcgacgcc gggattcccg aacttctccg
     6601 cattgccgat gaggtttgcg aagacctccg cgaggagatc gtcggccatg acctcgacgg
     6661 gtcttccctc gtagatgatc ccggcatcgg gatggtgcgc gattccggcc ctgatgacgg
     6721 cgtcgaggtc gacgggcctc gtcaccgtct cgctggagcg caatcttcgg agcgtcgaga
     6781 cgttctggat gatctcgatg ctccggttga ccccgcccct gagtttcaag gtcatctccc
     6841 tctccttccc ctccagcata tcggtgagca gatcggtgta gccgagggcg acggcgtttg
     6901 cgttgttgat gtcgtgcacc atgatatcca ggtagaggtt tgcctcctcg tgggctgccc
     6961 gcagttcccc ttccacccgg atgcggccgg tgatatcgga gcctgaaccg atgatcccga
     7021 ccggtctgcc gtcattgccg gtgagcaccg tatcctgcca tgcgaggatg agctcctccc
     7081 cgctccgggt gacgaccggg ctctcccgtg gatcggggta cgcggtgccg ctggcgatcg
     7141 cggcatcgaa gatctctcgc cgcgcctctt gatatgcttc agggacaacg gttgcaaacc
     7201 agtctttccc gagcagctcc tcctcgcagt agccgagcaa ctcgcaacct cggcggttga
     7261 tgagccggat ggtatgatcg gcgttgatga cgacgaagag gccgcccgcc acgtcgaggt
     7321 agtgccggac gagatccctc tctttctcca cctcccgttc ccgcctcttg agctgcccgg
     7381 agagggaggc gacgacgaga gcgatgagga cgaacatcag ggcgcgccca taatcgttca
     7441 atgtcaggat atccggcctg tagaggaggc tgctcacaac gacgagcccg ccgaggaaga
     7501 gagcgaccag cacgctccgc ttctcccacc agagtgcggt caggatgatc gggacgtaga
     7561 agaaatgcga gaagacggtc ccgagcatga ggacggcatg gaagtagtag gtcaggaaga
     7621 ccgatatggc aagaagggtg atgaggacac ccactcgctg ctgtttggta agggcaatca
     7681 cgggggatcc accttgacta ctgatatata taattttaaa atataaaaaa ttcgctatac
     7741 aaatgattgg ccggttctaa aggtttatcg gcggtttcga gaggataaaa gcgtcgccgg
     7801 gggagagcgg acataccgcg ccggccgggg tttccgaaga acggagaccc cggtccgaaa
     7861 ggtcgtggag atgcggtgcg gccctggatt gctctgatcc atcccccgga ggaccccgtg
     7921 caagctcgca tccggataac gatgcggttt tgcggaacgg ggggtgaggc gctcccggca
     7981 atccaccccg gactgtcacg gagaccgcgg atattcgggt tttagccccg aaatgtgcta
     8041 aaaactgcct atgtttaata cctc
//
LOCUS       DAOSII010000085         7971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_8411_length_7971_cov_40.006822, whole genome shotgun sequence.
ACCESSION   DAOSII010000085 DAOSII010000000
VERSION     DAOSII010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7971
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8411_length_7971_cov_40.006822"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..845
                     /locus_tag="PLV90_07190"
     CDS             147..845
                     /locus_tag="PLV90_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOF96937.1"
                     /translation="MLGTATIDVDATRIEVRPTDVRRIPQAGEAYDVVEIETDRGTTT
                     CRYYEAEGARAGVVMVGGVGGGFDTPGRGLYPRLAEGLQNDRIGALRVRYRHPTNLVE
                     SVVDTAIGMRYLESRGIRAIGLVGHSLGGAVVIQAAAGDPLVRVVVALSTQDAGTGPV
                     ARLAGRAAVLLIHGDADTVISPRSSEEVYRRTHEPKRLVIYEGAGHALDEAAESLAVE
                     VKAWLLKHLPGTLV"
     gene            809..2050
                     /locus_tag="PLV90_07195"
     CDS             809..2050
                     /locus_tag="PLV90_07195"
                     /inference="COORDINATES: protein motif:HMM:NF015963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HOF96938.1"
                     /translation="MAPQAPARDARLTPGTARMWENWSGSLRFSPERVERPESESAIR
                     DIVRRAAGAKKTLRPAGTGHSSSLLLATRDVLISLDAFRGIESYDPAKREATLLAGTT
                     LGEAGNLLLALKLAMHNLGDVDVQSVAGALATGTHGSGKHLPALSAMVAGGRMITASG
                     EKVEFSLEKDPDLTRAAQVSLGALGIYTALTLRLLPAYRLHRREWCTTADRCFAHIDD
                     LAERHRNFDFYWYPRSDRVKLRTMDPPGDAPESIPYARLVEDRTGWSSEVIAKVRTLR
                     FDEMEYAVPAEVGPECFEAVRRRIRERHRKHVAWRVLYRTVAPDDAYLSIAHGRATAT
                     ISLHQNATLPFWGFFNDIEPIFQEYGGRPHWAKKHTMAADDLMPLYPRWDRFIAARRR
                     MDPDGLFLNPYLERLLGEEKA"
     gene            2047..2421
                     /locus_tag="PLV90_07200"
     CDS             2047..2421
                     /locus_tag="PLV90_07200"
                     /inference="COORDINATES: protein motif:HMM:NF018767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HOF96939.1"
                     /translation="MRPAGRKVWAIPGGHIPLRTTGPEPEETSSDTVCLLNTGDRDAA
                     VEIRVYYEDREPVGPYRLTVPARRTRHIRFNDLIDPEPIPLDTDFAAVVISDLPIVVL
                     FARKDTSRAENAIAATLAFPCD"
     gene            complement(2434..3117)
                     /locus_tag="PLV90_07205"
     CDS             complement(2434..3117)
                     /locus_tag="PLV90_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOF96940.1"
                     /translation="MLIIGHRGARALAPENTLPALSKGMACADLVEIDVRLTRDGIPV
                     AIHDATVDRTTDGTGPVKGYTIEELKRLDAGEGEAIPTLREVLDLVHGQMGLIVEIKE
                     PGTEAIVVSVLMDRMPQRLILISFNPESVAMVKRLLPGVRAGLISTAEVDDPVGLARR
                     VQADLILPRWDRLSREVVERAHGAGLSVIPGTLNREDDLDEAVRLGVDGFATDDPCAA
                     RRYLEERRG"
     gene            complement(3253..3507)
                     /locus_tag="PLV90_07210"
     CDS             complement(3253..3507)
                     /locus_tag="PLV90_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOF96941.1"
                     /translation="MAGMKESATGMASGVAGTIQELDPQIIEVSTSGINYPASKSDLI
                     TRAKGAEAPQIVLDALDKFEDRQYKDPEDVKSEFQKIVKK"
     gene            3868..4605
                     /locus_tag="PLV90_07215"
     CDS             3868..4605
                     /locus_tag="PLV90_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOF96942.1"
                     /translation="MVALLAVIGAGCTGTTDDQTYVVGIDGSYVPFSYIDKDGNAQGF
                     DVDSMRWIAEKKGINVKFEAVEWSAIIPTLQAGKIDMVYAGMSITPERLEKVNFSNPY
                     WTVNQDVAARKDSNVTIEDVRAGKVVLGAQSGCTGAHWIEENVVGEGLMPKENLKLYV
                     DTQAAVSDLEIGRIDAVMYDDVSLREIIEGKPLKIIGSVQTQEQEQFGVAIRKDDTAL
                     LEFMNEALAELQADPYWEELKEKYNLK"
     gene            4696..5784
                     /locus_tag="PLV90_07220"
     CDS             4696..5784
                     /locus_tag="PLV90_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOF96943.1"
                     /translation="MESGSTLSHYRGPVLCALITAVLIGGSAPFTKLLIGGTGPLALA
                     ALVSLGSGAGALLFSLAGTRRSHMEAPPGRKEIPWLIGVTIFGGILAPVTLIQSLPGT
                     PAATAALLLNFEAVATTLIAAAVFRESVSRRVWIALGCITASCILLTRDPAAGFGLSL
                     PALGVLLTCLFWAVDNNMGQRLSAKDPLIIISVKGLVAGTITLIFALAAGEHLPDPAT
                     AAAAMAVGFLCYGGLTSILFLLALRGIGAARAGSLLAISPFFGVLFSLALFAELPAGA
                     FYAALPVMAAGAWLLVSEEHAHLHRHPPVVHEHRHRHDDLHHDHPHATGDPPLSPSGE
                     HSHLHEHGEIVHEHPHQPDIHHRHPHRR"
     gene            complement(5823..6509)
                     /locus_tag="PLV90_07225"
     CDS             complement(5823..6509)
                     /locus_tag="PLV90_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOF96944.1"
                     /translation="MDQATFLFDILLPALMEGLVITLQLIVCSAPFGLALGVAVATGR
                     QYGGRIISPLCKGFVYLIKGTPLLLLLFILYFGLPSIGITFSAFVASVIGFILCNGAY
                     NSEYIRGALMSIKDGQMIAAQALGMTRSQAIKSVILPQALRRAIPGLSNEFIYLIKYS
                     SLAYMITVIELSGAGKLVATKYFTYTESFAVVGIVYLALVSITTVAVNILEKQLAVPG
                     TVRATAAVQL"
     gene            complement(6516..7271)
                     /locus_tag="PLV90_07230"
     CDS             complement(6516..7271)
                     /locus_tag="PLV90_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOF96945.1"
                     /translation="MNNNDCVLRVEDIHKSFGGKEVLRGITFDVRRGETKVFIGPSGT
                     GKSTLLRCINQLTLPDRGRVWLDGEEVTNSGARINYFRQKIGMVFQNFYLFDHLTAVR
                     NVELALLKVRGMGKAEAREKALIELRQVGMEDWADHYPAELSGGQAQRVSIARALAMD
                     PDVILFDEPTSALDPELTREVLEVMKRLAREGMTMLVVTHEMGFACSVANEVLFMENG
                     VVAEQGAPDLLLRDPRFTRMKDFIGRLDDRPGD"
     gene            complement(7275..7952)
                     /locus_tag="PLV90_07235"
     CDS             complement(7275..7952)
                     /locus_tag="PLV90_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOF96946.1"
                     /translation="MDVLSILIDWFPYLLSGVTVTIGLVIAALGIGIILGLPMAIGQV
                     YGSRPVRSLIGVYVWFFRGLPILVLLFLFWFGIFPGIGLGHLPAFIVGAVVLGLRGAA
                     YQSQIFRGGIQSVGEGQMIAARSLGMSRLTAIRSVILPQAMRIALPGWSNEYPNILTD
                     SAVCYAIGVAELLTLASRIVAQTNVMMPIYLATAGIFIILNYGGLKILHGLERRVEIP
                     GFGTGAV"
BASE COUNT         1457 a         2665 c         2516 g         1333 t
ORIGIN      
        1 tctccgccgg cgggaacccg ggttcctccc cgccatcccc gccccggggc ttgcccatac
       61 caaaaccccg cctgggatct gcaacggtta gaccgcatgg aagcggcagc cacaaaggac
      121 aaatagcacc gtatccactc tcctccatgc tcgggacggc tacgattgat gtggatgcta
      181 cgcggattga ggttcgcccc acggatgtaa ggaggatccc ccaggccggc gaggcctacg
      241 atgtcgtgga gatcgagacc gaccggggga cgaccacctg ccgctactac gaggcggagg
      301 gggcacgcgc cggcgtcgtc atggtgggtg gtgtcggcgg cgggtttgac accccggggc
      361 gggggctcta cccgcgcctg gcagaaggcc tccagaacga caggatcggc gcactccggg
      421 tgcggtaccg gcacccgacc aacctcgtgg agtcggtcgt cgataccgcc atcggtatgc
      481 gctacctgga gagccggggc atcagggcca tcgggctcgt cgggcactcc ctcgggggag
      541 cggtggtgat ccaggcggcg gcaggcgacc ccctggtccg ggtcgtcgtc gccctctcga
      601 cccaggacgc cgggaccgga ccggtcgccc gccttgcggg ccgggcggcg gtcctcctga
      661 tccacgggga tgccgacacg gtcatatctc cccggtcgtc ggaggaggtc taccggcgca
      721 cccacgagcc caaacggctg gtcatctacg agggggcggg ccacgccctc gacgaggcgg
      781 cggagagcct cgccgtcgag gtgaaggcgt ggctcctcaa gcacctgccc gggacgctcg
      841 tctgaccccg gggacggcac gcatgtggga gaactggtcg gggagcctcc gtttttcacc
      901 ggaacgggta gaacggccgg agagcgagag cgcgatccgc gacatcgtac gccgggcggc
      961 aggagcgaag aagacgctcc gccccgccgg gaccggccac tcgtcgtcgc tcctcctcgc
     1021 gacccgggat gtactcatct cgctcgatgc cttcaggggc atcgagtcgt atgaccctgc
     1081 aaagcgcgag gcaacccttt tagccggcac gacgctcgga gaggccggga acctcctcct
     1141 cgccctcaag cttgccatgc ataacctcgg cgacgtcgac gtccagagcg tcgcgggcgc
     1201 cctggcgacg gggacgcacg gcagcggaaa gcacctgccg gccctctccg ccatggtcgc
     1261 cggcgggcgg atgatcacgg catcgggcga gaaggtggag ttctctcttg agaaggaccc
     1321 ggacctcacc cgggcggcac aggtctcgct cggagcgctc ggcatctaca cggccctgac
     1381 gctccgcctg ctcccggcat accggctcca ccgccgggag tggtgcacca ccgccgaccg
     1441 ctgcttcgcc cacatcgacg acctcgccga gaggcaccgg aacttcgact tctactggta
     1501 ccccaggagc gaccgggtga aactcaggac gatggatcct cccggagatg cgccggagag
     1561 catcccctat gcccgcctcg tcgaggacag gaccgggtgg agcagcgagg tcatcgcaaa
     1621 ggtccgcacc ctccggttcg acgagatgga gtatgccgtc ccggcggagg ttgggcccga
     1681 atgcttcgag gcggtccgcc ggcgcatacg ggaacgccac agaaaacacg tggcctggag
     1741 ggtcctctac cggacggtcg cgccggacga cgcctacctg agcattgcac acggccgcgc
     1801 gaccgccacg atatcgctcc accagaacgc gacgctgcct ttctggggct tcttcaacga
     1861 tatcgagccg atattccagg agtacggggg gcggccgcac tgggcgaaga aacacacgat
     1921 ggcggccgac gacctcatgc cgctctatcc ccggtgggac cggtttattg ccgcccgccg
     1981 ccgcatggac ccggacggcc tcttcctcaa cccctacctg gagaggctgc tcggggagga
     2041 gaaggcatga ggccggcagg gaggaaggtc tgggcgatac ccggcgggca tatccccctc
     2101 cggacgaccg gcccggagcc ggaggagacc agcagcgaca cggtctgcct gctcaacacc
     2161 ggggaccggg atgcggcggt cgagatcagg gtctactacg aagaccgcga gcctgtcggc
     2221 ccttaccgcc tcacggtgcc ggcccggcgg acccggcata tccgcttcaa cgacctgatc
     2281 gaccccgaac ccataccgct ggataccgat tttgccgctg tcgtcatatc ggatcttcct
     2341 atcgtcgtgc tctttgcccg gaaagacacc agccgggccg agaacgctat cgcagccacg
     2401 ctcgcgttcc cgtgcgactg atcggccggc ggatcaccct ctcctctcct ccaggtagcg
     2461 gcgtgcggcg caaggatcgt cggtcgcaaa accgtcgacg ccgagccgga cggcctcatc
     2521 gaggtcgtcc tccctattca gcgttcccgg tatgacagat aacccggccc cgtgcgcccg
     2581 ttccaccacc tcgcgcgaga ggcggtccca tcgcgggagg ataaggtccg cctgcacccg
     2641 gcgggcaagc ccgacgggat cgtcgacctc cgcagtggat atgaggcccg cccgcacgcc
     2701 ggggaggagc ctttttacca tcgcgacgct ctccgggtta aacgagatca ggatgagcct
     2761 ctgcggcatc cggtccatga gcaccgagac gacgatcgcc tcggtcccgg gctccttgat
     2821 ctcgacgatg agccccatct gcccgtggac gaggtcgagg acctcacgga gcgtcggtat
     2881 cgcctcaccc tcgcccgcat cgagcctctt cagctcttca atggtgtagc ccttgaccgg
     2941 cccggtccca tccgtcgtcc ggtcgacggt cgcgtcatgg attgcgacgg ggatcccgtc
     3001 cctcgtgagg cggacgtcga tctcgacgag atcggcgcat gccataccct tcgagagggc
     3061 cgggagagtg ttttccggtg caagagcccg ggcgccgcgg tgtccgatga tgagcatagt
     3121 gtgccgtcta cccgggggat agatatagat ggcgggtctc cgttgcacgg ctctctcgag
     3181 agaccgccag attgcacagt cggaaatggg agccggcagg cgccacatcc cggtgcctgc
     3241 ggccagaaaa gttcacttct tcacgatctt ctggaactcg gacttcacat cttccggatc
     3301 tttgtactgc cggtcctcga acttgtcgag cgcatcgagg acgatctggg gtgcctcggc
     3361 cccctttgcc cgtgtgatca ggtcgctctt cgatgcagga tagtttatcc ccgaagtgct
     3421 cacctcgatg atctgcggat cgagctcctg tatggtcccg gcgacacctg acgccatgcc
     3481 cgtagcggat tccttcatac ctgccatata tctcaccagc gtatctgtgc ccccttccgg
     3541 gaggcgaccc ccagtgggta agatgcaata aatacctatc gcccgtacgg cggcgggcag
     3601 ggtgccctgt atcggccgga tacagccgaa cctgcgacat tggaaggcta cccgggggat
     3661 tcacggccga tcgtgagcct tggagagatg atcgcctgtc cggtgcgccg aaccagggga
     3721 cggcggccgg aacatgcacc gggaacggca tggctccggg atggggcaaa ccggctcttc
     3781 cccgggccag atcagaaggt tcttcttact tacctgacta accccttctc tatgagaatg
     3841 catggtattc ttacagtgct tgcctgtatg gtcgcactac tggcagtcat cggggcagga
     3901 tgcaccggca cgaccgacga ccagacctac gtcgtcggca tcgacggttc gtacgtgccg
     3961 ttcagctaca tcgacaagga cggcaatgcc caggggttcg acgtcgactc catgagatgg
     4021 atcgccgaga agaagggcat caacgtgaag ttcgaggcgg tagagtggag cgccatcatc
     4081 cccaccctcc aggccgggaa gatcgatatg gtctacgcgg ggatgtcgat cacccccgag
     4141 cggctggaga aggtcaactt cagcaacccc tactggacgg tcaaccagga tgtcgcggct
     4201 cgtaaggact cgaacgtaac gatcgaggat gtccgtgccg gcaaggtggt tctcggtgcg
     4261 cagagcggat gcaccggcgc ccactggatc gaggagaacg tcgtcggaga agggctgatg
     4321 ccgaaggaga acctgaaact ctacgtcgac acccaggccg ccgtcagcga ccttgagatc
     4381 ggcaggatcg atgcggtcat gtacgacgat gtctcgctca gggagatcat cgaaggcaag
     4441 ccgctgaaga tcatcgggtc tgttcagacg caggagcagg agcagttcgg cgtcgccatc
     4501 aggaaggatg acaccgccct ccttgagttc atgaacgagg cgcttgcaga gctgcaggcc
     4561 gatccctact gggaagagct caaagagaaa tacaacctga agtaacgggg actatgggga
     4621 ccgatcgtcc cctccctctt ttagatcttc aaactcccgg aggcctctct tctgtacccg
     4681 cgcaaacccg ccgccataga gagcggatcc actctctcgc actaccgggg ccccgtcctc
     4741 tgtgcgctga taacggccgt cctgatcggc gggagcgccc cgttcaccaa actcctcatc
     4801 ggcgggaccg ggccgctcgc gctcgctgcg ctggtctcgc tcgggagcgg cgccggcgcc
     4861 ctgctcttct ccctcgcggg gacccggcgg agccacatgg aggcgccgcc cgggaggaag
     4921 gagatcccct ggctgatcgg ggtgacgata ttcggcggaa tcctcgcgcc ggtcaccctg
     4981 atacagagcc tcccggggac gccggcggcg acggccgccc tcctcctgaa cttcgaggcc
     5041 gtcgcgacga cgctgatagc cgcggcggtc tttcgggagt cggtgagccg ccgcgtctgg
     5101 attgcgctcg ggtgcatcac cgcatcctgc atcctcctca cccgcgaccc ggcggcgggc
     5161 ttcggcctct ccctccccgc gctcggtgtc ctgctcacct gcctcttctg ggcggtcgac
     5221 aacaacatgg ggcagcggct ctcggcaaag gatcccctca tcatcatatc cgtaaaaggg
     5281 ctcgttgccg gcaccatcac gctcatcttc gctctcgccg ccggagaaca cctcccggac
     5341 cctgccacgg cagccgccgc catggccgtc gggttcctct gctacggggg tctgacgagc
     5401 atcctcttcc tcctcgccct ccggggcatc ggcgccgccc gggcgggctc gctccttgcc
     5461 atatccccgt tctttggcgt cctcttctcc ctcgccctct ttgcagaact cccggcgggc
     5521 gcgttctacg ccgcgctgcc cgtcatggcc gcaggcgcat ggctgctggt ctccgaggag
     5581 catgcccacc tccaccggca cccgccggtc gtccacgagc accggcaccg ccacgacgac
     5641 cttcaccacg accaccccca cgcaaccggc gacccgccgc tctccccgtc cggtgagcac
     5701 tcccacctgc acgaacacgg cgagatcgtc cacgagcatc cgcaccagcc cgacatccat
     5761 caccgccacc cgcaccggcg ataacgcgga cacctgcaaa aagagggtgg cagccggatg
     5821 tcctagagct ggaccgcggc ggtagcccgg accgtcccgg gaacggccag ctgtttctcg
     5881 aggatgttga ccgcgacggt cgttatcgag accagcgcga ggtagacgat gccgacgact
     5941 gcaaacgact ccgtgtaggt gaagtacttc gtcgccacca gttttcccgc cccggagagc
     6001 tcgatcacgg tgatcatgta ggcaagcgac gagtacttga tcaggtagat gaactcgttt
     6061 gagagcccgg gtatcgcccg gcggagcgcc tgcggcagga tgacgctctt gatcgcctgc
     6121 gagcgggtca tgccgagagc ctgggcggcg atcatctggc catccttgat cgacatgagc
     6181 gcgccacgga tgtactcgga gttatacgcc ccgttgcaga ggatgaaccc gatcaccgat
     6241 gcgacgaacg cggagaacgt gatgccgatc gacgggaggc cgaagtagag gatgaagagg
     6301 aggaggagga gcggcgtacc cttgatgagg tagacaaaac ccttgcagag aggagatatg
     6361 atgcggccgc cgtactgcct ccccgttgca acggcgacac cgagcgcgag gccgaacggt
     6421 gcggagcaca cgatcagctg cagggttatg accagcccct ccatcagcgc cgggaggagg
     6481 atatcaaaga ggaaggtcgc ctggtccatc ccgcctcagt ctcccgggcg gtcatccagc
     6541 cggccgatga agtccttcat gcgggtgaac cgcggatcgc gcaggagcag gtccggcgct
     6601 ccctgctcgg ccacgacgcc gttctccata aagaggacct cgtttgcgac ggagcaggca
     6661 aaccccatct catgggtgac gacgagcatc gtcatcccct cccgggccag gcgcttcatg
     6721 acctcgagca cctcccgggt cagttcggga tcgagcgcgg acgtcggttc gtcaaagagg
     6781 atgacgtccg ggtccatggc gagcgcccgg gcgatggaga cccgctgggc ctggccgccg
     6841 gagagctcgg ccgggtagtg gtcggcccag tcctccatcc ccacctgccg gagttcgatg
     6901 agcgctttct cgcgggcttc cgcttttccc atacccctga ccttcaggag cgccagctcg
     6961 acgttcctga ccgcggtgag gtggtcaaag agatagaagt tctgaaagac catcccgatc
     7021 ttctgccgga agtagttgat ccgggctccc gagttcgtga cctcttcgcc gtcgagccag
     7081 acccgcccgc ggtcgggcag cgtcagctgg ttgatgcacc gcaggagcgt gctcttgccc
     7141 gtccccgaag ggccgatgaa gaccttcgtc tcgcccctcc ggacgtcaaa cgtgatcccc
     7201 cgcagcacct ccttgccccc gaaggacttg tgtatatcct caactctcag aacgcagtcg
     7261 ttgttgttca tgctctacac cgctccggtc ccgaatcccg ggatttccac ccgcctctcc
     7321 agcccatgca ggatcttcaa gcccccgtag ttcaggatga tgaagatccc tgccgtggca
     7381 agatagatcg gcatcatcac gttggtctgc gcgacgatcc tcgacgcaag ggtgaggagc
     7441 tcggcgacgc cgatggcgta acagaccgcc gaatcggtca ggatgttggg atactcgttc
     7501 gaccacccgg gcaacgctat gcgcatcgcc tgggggagga tgacggagcg gatggcggtg
     7561 agcctgctca tcccgagcga tcgcgccgcg atcatctgcc cttccccgac cgattggatg
     7621 cccccccgga agatctggga ctggtacgcc gcaccccgga ggcccaggac gaccgctccg
     7681 acgatgaatg ccggaagatg gcctagaccg atccccggga atatgccgaa ccagaagagg
     7741 aagaggagga cgaggatggg gagacccctg aagaaccaga cgtagacccc gatgagcgat
     7801 cgtaccgggc gggagccgta tacctggccg atcgccatgg gtagcccgag gatgatgccg
     7861 atgccgagtg cggcgatgac aaggccgatg gtaacggtaa cgccggagag aagatacggg
     7921 aaccaatcta tgaggattga cagaacgtcc atttggctat tcctgggtca t
//
LOCUS       DAOSII010000086         7820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_8596_length_7820_cov_52.295557, whole genome shotgun sequence.
ACCESSION   DAOSII010000086 DAOSII010000000
VERSION     DAOSII010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7820
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8596_length_7820_cov_52.295557"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..395)
                     /locus_tag="PLV90_07240"
     CDS             complement(21..395)
                     /locus_tag="PLV90_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2703 domain-containing protein"
                     /protein_id="HOF96947.1"
                     /translation="MKKELIIEWRHAGSAVNKSPEGVEETGMTLAAVLAEIRMLLEME
                     GVTVRMIETDAEPEGLLFNGIPVERLLEGIEVTALSCSCASCDSCEGDAECRALRYNG
                     EAYEAIPPDLIGRAAVKALEVE"
     gene            complement(610..1509)
                     /gene="pdxS"
                     /locus_tag="PLV90_07245"
     CDS             complement(610..1509)
                     /gene="pdxS"
                     /locus_tag="PLV90_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOF96948.1"
                     /translation="MKLEELRFGTELIKRGFASMQRGGVIMDVVNAEQAELAEGVGAV
                     AVMALERVPADIRAAGGVARMADPQKIVEIIDAVSIPVMGKARIGHFVEAQVLEALGV
                     DMVDESEVLTPADEQFHIDKTRFEVPFVCGARNLGEALRRIDEGAAMIRTKGEAGTGN
                     VVEAVRHMRAIMGGIRSLKGLSSQELIDRAREIEAPAELVIECANLGRLPVVNFSAGG
                     IATPADAALMMQLGADGVFVGSGIFKSSNPEATARAIVEAVNHYDDAKVIAEVSRGLG
                     DAMKGIDIRVLREDEILQTRGRT"
     gene            complement(1515..2435)
                     /gene="cbiB"
                     /locus_tag="PLV90_07250"
     CDS             complement(1515..2435)
                     /gene="cbiB"
                     /locus_tag="PLV90_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HOF96949.1"
                     /translation="MAAAAVVVAASLLVDRLVGDPHSRYHPVALLGRFIGWWGVPSRY
                     PARVQRAAGAAGTILTVALFAAPFALVQFAAPWYLSIILAPFLLKACFAWRSLEEHTV
                     AVVQALEDGGIDAGRARAGMLVSRDTAHLEPEHILSAAYESMTENLVDSIISPLFYFG
                     FFGLAGAAAFRAINTMDAMLGYRDERLRIGWFPARADDIANFIPARITGLVLLVYFAA
                     KGRFSPAYAVLCRDRKKRPGFNGGIPMAVIAGGVGVRLEKPGVYAIGDAERTLTEAGA
                     GIVSAVRAATIIFALAEIGALFLLVSMRYT"
     gene            complement(2414..2932)
                     /locus_tag="PLV90_07255"
     CDS             complement(2414..2932)
                     /locus_tag="PLV90_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96950.1"
                     /translation="MSEIHLNRRGINAIEVPGEVEAVPGSDLSLRIINHGSPLHISLA
                     TTNSAPFTDFIHENLYVGGTDAEFHISIREDAPPGVFSVEVIAGYGARRAAFKVFVRE
                     RKAPEPGPVAAPRAEPARTVSLPPLPVLAPVVAALVLYGLWLASGIDLLNAAAFAVLL
                     VGVILAWLRQRS"
     gene            complement(2941..3126)
                     /locus_tag="PLV90_07260"
     CDS             complement(2941..3126)
                     /locus_tag="PLV90_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methytransferase partner Trm112"
                     /protein_id="HOF96951.1"
                     /translation="MKRELMDILCCPVCKGDLLLSATEESGGEVLEGTLRCAACRVDY
                     PISEGIPNLLPQTACED"
     gene            complement(3123..4133)
                     /locus_tag="PLV90_07265"
     CDS             complement(3123..4133)
                     /locus_tag="PLV90_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HOF96952.1"
                     /translation="MSCTIIVGGFFGDEGKGKIVAHIAHQDRPSIISRGGVGPNAGHT
                     VCVGEKEYGVRMVPSGFVYPEAALMIGSGVLVDPRVFLREVDLLGVRGRIFVDGRCGV
                     IEEEHIARDRGSEHLNKKIGSTGTGCGPANSDRVLRTSRQAKDVPELAEYITDVAEDL
                     NCAMDRGEVVLLEGTQGFGISLYFGTYPFVTSKDTSASQIAADNGVGPTRIDDVIVVF
                     KAYPTRVGEGPFATEMTMERSHELGIEEFGTVTRRQRRIGTWDGKMARYSAMINGCTQ
                     AAITGIDRIDPACFGVKDYDRLTRKAKDFIAQAEEDIGKPVTLISTGPEMSQIIDLRS
                     EL"
     gene            complement(4801..5961)
                     /locus_tag="PLV90_07270"
     CDS             complement(4801..5961)
                     /locus_tag="PLV90_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOF96953.1"
                     /translation="MRDFVSARVRAIPPSGIRKFFDIAQTMEDVISLGVGEPDFVTPW
                     CVCEASIYSIEQGSTAYTSNKGTPQLRGAISRYLDTRFGTCYDPDEEVIVTCGVSEAA
                     DIAIRAVTDPGDEVLVAEPSYVSYTPCVSLAGGVPVTVPCRAEDEFRLTADALMERIT
                     PRTKVLVANFPNNPTGGVMPEADWRAIADILVDHDLLLISDEVYAELTYDGDHFSPAK
                     IPELRDRTITLNGFSKAFAMTGWRIGYLCAPPALAAAALKIHQYVALCAPVMGQVAAY
                     EALRNGEAEKDAMVQEYRLRRNLFVDGLNKLGLACHLPKGAFYAFPSIESTGMTDTEF
                     AESLLYEQHVAVVPGSVLGPSGAGHVRCAYAVSRDNLKKALSRMGAFLESAK"
     gene            complement(5973..6464)
                     /locus_tag="PLV90_07275"
     CDS             complement(5973..6464)
                     /locus_tag="PLV90_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOF96954.1"
                     /translation="MDDKDRILLQLLEENCRTPTNELAVLAEVSEQDIRDRIDRLEAA
                     GVIRRYGAVVDWERAGNGNVAAVIELKVSPERDFGYDRIAERIARFPAVRSLRLMTGA
                     YDLQLLVVGSSMHEIARFVAEQIAPMDRIRETATHIIMKTYKENGTTYAGREEVERLP
                     YSF"
     gene            complement(6469..7752)
                     /locus_tag="PLV90_07280"
     CDS             complement(6469..7752)
                     /locus_tag="PLV90_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORC1-type DNA replication protein"
                     /protein_id="HOF96955.1"
                     /translation="MSDDKTNPMGLFKKYLTNKRIFKNREVLRHSYRPHILPHRKPQI
                     DELASILAPALTNETPSNILIYGKTGTGKTASVRYVGTELENASALAGTKCRIVHLNC
                     EVIDTQYRVLAQIANCLDDADQHPSDSPRSLIPMTGWPTDQVYMELKNQLESNGGVMV
                     IILDEIDKLVKKSGDDTLYNLTRINSDLKFAKVSIIGISNDLRFTDFLDPRVLSSLSE
                     EEIVFPPYNAPQLCDILQQRADMAFIEGALGETVIPLCAALAAQEHGDARRALDLLRV
                     SGELADRENAAGVGEKHVRMAQEKIETDSMVECVSTLPTQSKAVLYAMLILEQMGKRI
                     FTSGEVTVVYREIARLIDLDVLTHRRITDLISELNMLGVINTRVVSRGRYGRTKEMWF
                     DATTSKIEEVITRDPRLVDQRLKELDINRLRAMFR"
BASE COUNT         1406 a         2468 c         2477 g         1469 t
ORIGIN      
        1 tggcgggctc cgccacgccc tcactccacc tcaagcgctt ttacggccgc ccggcctatc
       61 aggtccggcg ggatcgcctc gtacgcctcg ccgttgtaac ggagcgcccg gcactccgcg
      121 tctccctcac aggagtcgca ggatgcgcag gagcaggata gggcagtcac ctcgatccct
      181 tcgaggagtc tctcgaccgg aatgccgttg aagaggaggc cctccggttc tgcatccgtc
      241 tcgatcatcc ggacggtgac gccctccatc tcaaggagca tgcggatctc cgcgagaacg
      301 gccgcaagag tcataccggt ctcctcaacc ccttccgggg acttgttgac cgcgcttccg
      361 gcatgtctcc attcgatgat gagttccttc ttcatggtgc acctccacta ttggcatcga
      421 tgagacatct tcctgccgct gcggtcacat cctccgcggc atgctccgcc tgtgcaggtg
      481 catcgcggcg gcggtaaaga ccagggtgaa cccggcaaac gccgcgatat cgaccaccac
      541 ggggaagtgg catggcgccg gagaacgagt accgcaccag atctttgcag cacgcaccgg
      601 tatgaggtct caggtccgcc cgcgcgtctg gaggatctcg tcttcccgga gcacgcggat
      661 atcgatgccc ttcatcgcgt ccccgagccc ccggctcacc tcggcgatga cctttgcgtc
      721 gtcgtagtgg ttcaccgcct ccacgatcgc ccgggcggtc gcttcggggt ttgacgactt
      781 gaagatcccc gacccgacaa agacaccgtc ggccccgagc tgcatcatga gcgccgcatc
      841 cgcgggcgtc gcgatgccgc cggccgagaa gttcaccacg ggaaggcggc caaggtttgc
      901 gcactcgatg acgagttctg ccggcgcctc gatctcgcgg gcccggtcga tgagctcctg
      961 ggaagagagc cctttcaggc tccggatccc gcccatgatc gcccgcatgt ggcggaccgc
     1021 ttccacgacg ttccccgtcc cggcctcgcc cttcgtccgg atcatcgctg ccccctcgtc
     1081 gatccggcgc aacgcctccc cgaggttcct cgcaccgcag acgaacggga cctcaaaccg
     1141 ggtcttatcg atgtggaact gctcgtccgc cggggtcagc acctcgctct cgtcgaccat
     1201 gtccacgccg agcgcttcca gaacctgtgc ctcgacgaaa tggccgatcc gggccttccc
     1261 catcaccggg atggagaccg catcgatgat ctcgacgatc ttctgcgggt cggccatgcg
     1321 ggccacaccc cctgccgccc tgatatcggc agggaccctc tcgagcgcca tgacggcaac
     1381 ggcaccgacc ccttcggcga gctctgcctg ctctgcgttg acgacatcca tgatgacgcc
     1441 gcctctctgc atcgacgcaa agccgcgctt gatcagctcg gtgccaaatc tcagttcctc
     1501 aagtttcatt acctctatgt atagcgcatg gataccaata aaaataatgc gccgatctcg
     1561 gcaagggcga agatgatcgt cgccgcccgg accgcagaga cgatcccggc gcccgcctcg
     1621 gtaagtgtcc gctccgcatc cccgattgcg tagacgcccg gtttctcgag ccggacgccg
     1681 acgccgccgg cgatgacggc catcggtata ccaccgttga acccgggacg cttctttcta
     1741 tcccggcaga gcaccgcgta ggccggggaa aaccgcccct ttgcggcgaa atagacgagg
     1801 aggacgagac cggtgatgcg cgccggtatg aagtttgcaa tgtcgtcggc gcgggccggg
     1861 aaccacccga tccggagccg ctcgtcgcgg taaccgagca tcgcgtccat ggtgttgatc
     1921 gcccggaaag ccgcggcccc tgcgaggccg aagaacccga agtagaagag cggggagatg
     1981 atgctatcga ccaggttctc ggtcatcgat tcgtacgcag ccgagaggat gtgctcgggt
     2041 tcgaggtgcg ccgtatccct gctcaccagc atgccggccc gggcgcgccc ggcgtcgatg
     2101 ccgccgtcct cgagcgcctg caccacggca acggtatgct cctcaagcga tcgccaggcg
     2161 aaacaggcct tgaggaggaa gggcgcgagg atgatggaga ggtaccaggg ggcggcgaac
     2221 tggacgaggg caaacggggc ggcaaagagt gccacggtca gaatggtccc cgcggccccg
     2281 gccgcccgct ggacacgggc cgggtagcgg gacgggaccc cccaccaccc gatgaaccgg
     2341 ccgaggagcg ccaccgggtg gtatcgggag tgcggatccc cgaccagccg gtcaaccagg
     2401 agcgacgcgg cgactacgac cgctgccgca gccatgcgag gatgaccccg acgaggagca
     2461 ccgcaaacgc ggcggcatta aggaggtcga tccctgatgc aagccagagg ccgtagagca
     2521 ccagggcggc gacgacgggg gcgagaaccg ggagaggcgg gagcgataca gtccgggcgg
     2581 gctccgcccg gggggcagca accggcccgg gctcgggggc tttccgctcg cggacgaaga
     2641 ccttgaacgc cgcccgccgg gcgccgtatc cggcgatgac ctccacggag aagacacccg
     2701 ggggagcatc ctctctgatg gagatatgga actccgcgtc ggtgccgccc acatagaggt
     2761 tctcgtggat gaagtcggta aacggcgcag agttcgtcgt ggcgagagat atgtggagcg
     2821 gagagccgtg gttgatgatc cggagggaga gatcgcttcc cggcaccgcc tccacctcgc
     2881 ccgggacctc gatggcgttg atcccgcggc ggttgagatg aatctccgac atcggcggga
     2941 tcagtcctcg caggccgtct gcgggaggag gtttggtatg ccttcgctga tggggtaatc
     3001 gacgcggcat gccgcgcacc ggagggtgcc ctccagcacc tccccgccgc tctcctcggt
     3061 cgcagagagc agaagatcgc ccttgcagac cgggcagcag aggatatcca taagttcccg
     3121 cttcatagtt cactccggag atcgatgatc tgggacatct cagggcccgt cgatatcaat
     3181 gtgacgggtt tgccgatatc ctcctcggcc tgtgcgatga agtcctttgc cttccgggtc
     3241 agtcgatcat agtccttcac gccgaagcag gccgggtcga tccggtcgat gccggtgatc
     3301 gccgcctggg tacacccgtt gatcatggcc gagtagcggg ccatcttccc gtcccatgtg
     3361 ccgatacggc gctgacggcg ggtgacggtg ccgaactcct cgataccgag ttcatgtgat
     3421 cgctccatcg tcatctcggt cgcaaagggg ccttcgccga cccgggtcgg gtaggctttg
     3481 aagacgacga tgacgtcgtc gatccgggtc ggaccgacgc cgttgtcggc ggcgatctgc
     3541 gaggcggacg tatctttgct ggtcacgaac gggtaagtcc cgaagtagag cgatatgccg
     3601 aaaccctggg ttccctcaag gaggaccacc tcgccccggt ccatggcgca gttgaggtct
     3661 tccgccacat cggtgatgta ctccgcaagt tcggggacat cttttgcctg ccgcgacgtc
     3721 cggaggacgc ggtcggagtt tgccgggccg catccggtcc cggtgctccc gatcttctta
     3781 ttgaggtgct cgctccccct gtcgcgagcg atatgctcct cttcgatgac accgcaccgg
     3841 ccgtcgacaa agatcctgcc cctgacgccg agcagatcga cctccctcag gaagacccgg
     3901 ggatcgacga gcacaccgct ccctatcatg agggccgctt ccgggtacac gaaccccgag
     3961 gggaccatcc tgacaccata ctccttctcc ccgacacaga cggtatgccc ggcattcggg
     4021 ccgacgccgc cccgggagat gatggatggt ctgtcctggt gagcgatgtg ggccacgatc
     4081 tttccttttc cctcgtcacc aaagaatccg ccgacgataa tagtacaact cattgttcta
     4141 tcatgtagga tggagcgata caccctgata aacgttacaa tgcaacactg agggtgcgat
     4201 cgtcctgcgt gaaagtagcc gggtttccgg ggtgcaggga ccatccaccc cacgttccgg
     4261 gcggatgagc gccggagggg atcgcatccc gcgcgaggct ggcggtcgcc cgggggagga
     4321 gcgcagatcc tggacaggcc attctggcgc tgcgccgcga tatgcgccta tcccggggtt
     4381 ccggcgacag gaagccattg tatatcaacc ttcgcggcgg cagatcgccc cggacccggt
     4441 cgacaaaggg gtctggaccc cttcatttcc actggaaact ctatctccat gagatccgga
     4501 gccgatacat cgagattttt ccggaaaagt catgttgatc tgtaatcgga tctttcagac
     4561 ggagcgcgga tgcgaacccg ggtaatctgc ccggggtttg tgggacggga tgcgtacccc
     4621 cgtatgaggg cgacggggtc ctgcggcacc tatgatccct gaataaatcc acatctccag
     4681 tggaaataaa ggggttctcc gcacgccgtt cccctgtcgg acaccggaca ggcagttgcc
     4741 ctgacaggca taccatataa aaaggttttg aatataccaa aagcgcgtat ggtgacgttt
     4801 ctattttgcc gactcaagga acgcccccat ccgggagaga gccttcttga gattatcccg
     4861 cgagaccgcg taggcgcacc gcacgtgccc ggcgccggac ggtccgagaa cgcttcccgg
     4921 gacgaccgcc acgtgctgct cgtagagaag agactcggca aactcggtat ccgtcatgcc
     4981 ggtgctctct atcgagggga aggcgtagaa ggcgcccttc gggaggtggc aggcaagacc
     5041 gagcttgttt aacccgtcga cgaagaggtt ccgccggagc cggtactcct ggaccatggc
     5101 gtccttctcg gcttcaccat tccggagcgc ctcgtaggcg gcaacctgcc ccatgaccgg
     5161 ggcgcagagc gcgacatact ggtggatctt cagggccgct gcggcgagcg ccggcggtgc
     5221 gcagaggtag ccgatccgcc acccggtcat tgcaaaggcc tttgagaacc cgttcaaggt
     5281 gatcgtccgg tcacggagct cggggatctt tgccggcgag aagtgatcgc cgtcgtaggt
     5341 gagctcggca tagacctcgt cgctgatgag gaggaggtcg tggtcgacca ggatatcggc
     5401 gattgcgcgc caatctgcct ccggcatcac cccgccggtg gggttgttcg ggaagtttgc
     5461 taccagaacc ttcgtccggg gagtgatacg ctccatgagc gcatcggccg tcagccggaa
     5521 ctcgtcctcc gcccggcagg gcacggtcac cgggacaccg ccggcgaggg agacacaagg
     5581 ggtgtaggag acgtagctcg gttctgccac cagcacctcg tcgcccggat cggtgacggc
     5641 gcggatggcg atatcggccg cctccgagac gccgcaggtc acgatgacct cctcgtcggg
     5701 atcgtagcat gtcccaaagc gggtatcgag gtagcggctg atagccccgc ggagctgggg
     5761 cgtccccttg ttcgatgtat aggcggtcga cccctgctcg atggagtaga tggacgcctc
     5821 acagacgcac cagggggtca cgaagtccgg ttcgccgacg cccagggata tgacgtcctc
     5881 catcgtctgg gcgatatcga agaacttccg gatgcccgaa ggcggtatgg cacgcacccg
     5941 tgccgagacg aaatctctca acgcacctca cctcagaacg agtagggaag gcgctcgact
     6001 tcttcgcgcc cggcgtaggt ggtcccattc tccttatagg tcttcatgat gatgtgcgtc
     6061 gccgtctcgc ggatccggtc catcggggcg atctgctcgg cgacgaaccg cgcgatctcg
     6121 tgcatgctcg acccgaccac caggagctgg agatcgtatg ccccggtcat caaccggagc
     6181 gaccggacag cggggaaccg ggcgatccgc tccgctatcc ggtcgtagcc gaagtcccgc
     6241 tcgggcgaga ctttgagctc gatcaccgcg gcgacgttgc cgtttccggc ccgttcccag
     6301 tcgacgaccg caccgtaacg gcggatgacg ccggccgctt ccagccggtc gatccggtct
     6361 cttatgtcct gctcactcac ttcggccagc accgcgagtt cgtttgtcgg ggtgcggcag
     6421 ttctcctcca ggagctggag gaggatacga tccttgtcat ccatggtttc acctgaacat
     6481 tgctcttaat cggttgatgt cgagttcctt gagcctctgg tcgacgagtc gagggtcgcg
     6541 cgtgatgacc tcttcgatct tgctggtcgt cgcatcgaac cacatctctt tcgtccggcc
     6601 gtagcgcccc cgcgagacga cccgggtgtt gatgacaccg agcatgttga gctcggagat
     6661 gaggtccgtg atccggcggt gcgtcaggac gtcgaggtcg atgagccggg cgatctcccg
     6721 gtagaccacc gtgacctcgc cgctcgtgaa gatccgtttg cccatctgtt cgaggatcag
     6781 catcgcgtag aggactgcct tgctctgggt cgggagggtg gagacgcact ccaccatgct
     6841 gtcggtctcg atcttctcct gcgccatccg gacatgcttc tcacccacgc ctgcggcgtt
     6901 ctcgcggtcc gcaagctcgc ctgagaccct gagaaggtcg agcgcccgcc gggcatcccc
     6961 gtgctcctgg gccgcgagcg ctgcgcagag cgggatgacc gtctcaccga gcgccccttc
     7021 gatgaacgcc atatcggccc tctgctggag gatgtcgcag agctgggggg cgttataggg
     7081 ggggaagacg atctcttcct cggagagcga ggagaggaca cgggggtcca gaaagtcggt
     7141 gaaccggaga tcgttggata tcccgatgat gctgaccttt gcgaacttga ggtcggagtt
     7201 gatccgggtg aggttgtaga gggtgtcatc gccgctcttc ttgacgagtt tgtcgatctc
     7261 gtcgagtatg atgaccatga ccccgccgtt gctctcgagc tggttcttga gctccatgta
     7321 gacctggtcg gtcggccacc cggtcatcgg gatgaggctc cgggggctgt cgctcgggtg
     7381 ctggtcggcg tcatcaagac agtttgcgat ctgggcgagg acgcggtact gggtatcgat
     7441 cacctcgcag ttgaggtgga cgatccggca cttcgtcccg gctaaagcgc tcgcgttctc
     7501 aagctccgtc ccgacatacc gaaccgaggc agtctttccg gtcccggtct tgccgtagat
     7561 gagtatgttt gagggagtct cgttcgtgag ggcaggcgca aggatagagg cgagttcgtc
     7621 gatctggggt ttccggtgag gaaggatgtg aggacgataa ctgtggcgca acacttccct
     7681 gttcttaaat atacgcttgt tggtcaggta ttttttgaat agtcccatcg ggttggtctt
     7741 atcgtccgac atgatgtcac cggagggggt tgtcaggaca ggagattctc acgccgtgaa
     7801 cccctaagat gatcgtcgtt
//
LOCUS       DAOSII010000087         7769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_8656_length_7769_cov_51.501685, whole genome shotgun sequence.
ACCESSION   DAOSII010000087 DAOSII010000000
VERSION     DAOSII010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7769
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8656_length_7769_cov_51.501685"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PLV90_07285"
     CDS             complement(<1..276)
                     /locus_tag="PLV90_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOF96956.1"
                     /translation="MNRSSAVRRDTGAIEARIGYVFQDRALLVRALTRLAYTLETGLP
                     PGAHMDALATLGDAVINVVVVEALVAEGAHDKGVITNRKVNLVNMTRL"
     gene            complement(287..772)
                     /locus_tag="PLV90_07290"
     CDS             complement(287..772)
                     /locus_tag="PLV90_07290"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOF96957.1"
                     /translation="MAEKTVVLHTTMGKIAIRLYGDMPVTAGNFAKLAGSGFYDGTIL
                     HRVIANFMIQGGDPTGTGMGGPGYTIADEFVKGHSNRRGTIAMANTGRPNTGGSQFFI
                     NLVDNTYLDWDDPRTPSAHPVFGEVVEGMDIVDKIGKVRTDSADKPKVPVRIEKAEVL
                     E"
     gene            971..1756
                     /locus_tag="PLV90_07295"
     CDS             971..1756
                     /locus_tag="PLV90_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HOF96958.1"
                     /translation="MYPGAKITADPRPGYTRITPFSLPPRQEPAGTNGRAPVFIITGE
                     QGQCKTTFLHLVLGLIVGANVRIRGVLAPGHVRDGRRSGFTLVDLATGEHEELSSVDP
                     DPRCEVHGRFYFRPEGLAFGRRALAPPNPRETDLMAIDEVGRFELQGAVWADQIDRLV
                     SLSHPPMVWTVRRRLLDTVIERWKITNPVVVDVRSASVMATAGDVMDAVWEWREAQTA
                     SAVPVSPFSPGRIAEGPPLLADAAAGASPGAPSIFRRRYGPEG"
     gene            2150..3334
                     /locus_tag="PLV90_07300"
     CDS             2150..3334
                     /locus_tag="PLV90_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOF96959.1"
                     /translation="MTRGEEPTVEVGQRPEGTIAIELPWVASRGGMPVYEAVLRIFPV
                     EKAIYILPIVLFVFSLFFGRYMMDPVETLRILIVGSATIFLDTASSLLSTFAGHQVLL
                     PSFEQAWGASEQTVIFQIRLPRVIAAMLVGGGLALAGASFQGLFRNPLVSPDILGVAS
                     GAGFGAALGILISGDPRVIQVSAFIFGIVAVAVTYGISRVYRNSSTLILVLGGIVVGS
                     VFSALLSLTKYVADPYDTLPAIVFWLMGSLSAVSNADLLAVAPPILLGGLCLFLIRWR
                     INLLSVGEEEAQALGVDTKKMTAAIIVASTVVTASAVCISGIIGWVGLVVPHIGRMLV
                     GPDFRRLIPVSAVLGASFLLVVDDIARTLTAAEIPLGVLTALIGAPFFAYLLTRKRVG
                     WS"
     gene            3331..4122
                     /locus_tag="PLV90_07305"
     CDS             3331..4122
                     /locus_tag="PLV90_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF96960.1"
                     /translation="MILNVEHASFSYDGITKQFEDISFSLRRGEVLSLLGKNGTGKST
                     LIKCILNILSLQSGKVEINGRRIAGMRPDEVAREVGYVPQVHRAAFPFTALDFVLMGR
                     APHISTFSVPKEEDYRKVEETFARIGITHLREKPLPEMSGGESQMVMIARALAQEPSL
                     LVLDEPTSHLDIGNQMKMIATINRLAAEGIGILMSTHFPDHGFMTSHTVAILENGRLI
                     AQGPAEDVITRENLYKAYGVDVCIRYIEEAGRMACIPINSCGVPG"
     gene            complement(4201..5907)
                     /locus_tag="PLV90_07310"
     CDS             complement(4201..5907)
                     /locus_tag="PLV90_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOF96961.1"
                     /translation="MIGPGGVGRCRMYTNEGGTIVERYPDRYIAVMPIAIETMPMCHY
                     YPRETFLQASGIGCTFSCGGCISETVALHAEAVSGALKHIPPDALIRRAREEGCRGIA
                     FCLNDPTAMHRTFLAVARAARDAGLLVGCATNGYYTEEALHDLAPAIDFVNIGLKGAS
                     DARYRACGAVSAEPAFRAVKTLHEAGVHVEVSCIYARGYDDEIRAVAARIADISPDIP
                     LLVMRFIPFGDESPEREPTIAESERICDDLRRRLRYVYLFNSPGTGYLDTVCPSCGER
                     VVGREFFGPMGARTIAVPPDARCLCGFSLPVLGSIGKTPYAEPGMMGGYRFTRALEMV
                     HAVLVCLGVESDADLARIWTGVIKDDYIRDLHGKIQTIDGYTGLVRDLGERSGRPEAA
                     EALAAYIGDRVAAVSSAVEGYPRPRVYYSMGTPLFALNADRFEMDLVEAAGGDPVNRR
                     IVRAGKPGVNITPEEFAAFNPEYIFISGFLSAPASDYLTACRRMGLSADAIEHGRVYT
                     LPPGWDFGNPRWVLGLSAIAGTLHPACAAFDLAAEEDRFYRTFYGAEASIRTGNRSFY
                     RP"
     gene            complement(5942..6583)
                     /locus_tag="PLV90_07315"
     CDS             complement(5942..6583)
                     /locus_tag="PLV90_07315"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOF96962.1"
                     /translation="MGYTPGRFDRDGSKNMNRIAKTVFAPVYPVIAEQIVERCRISRG
                     RCLDIGSGPGSLGIALARASDLTVTLFDSSSEMLALAEGNIREAGLSGRLSVLPGDVH
                     DIPLPAGSVDIAVSRGSIFFWEDLARAFSEIHRVLAPGGKTYIGGGFGTAELRDAVLA
                     AMTKENPDWKNFRDKNLGPENRERVVGVIEGLGLPHRIVNDDSGFWVMMEKGR"
     gene            complement(6594..7682)
                     /locus_tag="PLV90_07320"
     CDS             complement(6594..7682)
                     /locus_tag="PLV90_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOF96963.1"
                     /translation="MDVVVITKRCHLLLIMLGVCAVLLCASAACTTARESSPSAEDRT
                     ITDMVDRTVVVPSEIESVLCTSPPSTNLVYMVAPDRLKGWNFAPEKGYTAEKYMALPV
                     IGGWFGKETGNYETFISMHPDIVIEGFTTDGGAASATIAERQQKMEPIPVVAVEDSTN
                     STGYSAPIRFVGDLLGEEEQAEAMIAFYEGVLATVAERAASIPDEERVGVYYAEGPRG
                     LTTDPSGSSHSELIDLCGGRNIADCAITPGMGMTEVSMEQVIAWDPDVILVENPGFYA
                     AVGTDPAWQEITAVKTGRVYLTPRTAFCWFDRPPGINRIIGIPWTAKTLYPDLFRDMD
                     LESLVREYHEIFLHVSLSDDQIHQILNL"
BASE COUNT         1399 a         2519 c         2393 g         1458 t
ORIGIN      
        1 gaggcgggtc atgttcacga ggttcacctt ccggttcgtg atcacgccct tgtcgtgcgc
       61 cccctccgcg accagcgctt ccacgaccac gacgttgatg accgcatcgc cgagggtcgc
      121 gagggcatcc atgtgggcgc ccgggggaag acccgtctcc agggtgtaag cgaggcgggt
      181 gagcgcccgc acaaggagcg cccggtcctg gaatacgtag ccgatccgtg cctcgatcgc
      241 gcccgtgtcc cggcggacgg cgctgctgcg gttcatggca gaggggctac tccaggactt
      301 ccgccttctc tatccggacg gggaccttcg gtttatcggc ggagtccgtc cttaccttcc
      361 cgatcttatc gacgatatcc atcccctcga ccacctcgcc gaagaccggg tgggcgctcg
      421 gcgtccgggg gtcgtcccag tcgaggtagg tgttgtccac caggttgatg aagaactggc
      481 tgccgccggt gttcgggcgg ccggtgtttg ccatggcgat cgtgccccgg cggtttgaat
      541 ggcccttcac gaactcgtcg gcgatggtgt agcccggccc gcccatcccg gtgccggtcg
      601 ggtcgccgcc ctggatcatg aaattcgcga tgacccggtg gaggattgta ccatcataaa
      661 acccggatcc tgccagtttt gcaaaatttc ccgccgtcac cggcatgtcg ccgtagaggc
      721 ggatcgcgat cttacccatg gtcgtgtgga gcacgacggt cttctctgcc attggtataa
      781 ctccgtatgg tattgttcgc cgttgttact tatactggtg ggaggcgtat gccccaccgc
      841 cggcaggtga agatcgccat ccggcccggg ggcgggcatc cacccttccc ggcggatcac
      901 gtcaatggta ttacctaaac aaatagcata tgtaaagatg ttttatatgg gcggccgcag
      961 aagtatatgc atgtaccccg gagcgaagat cacggccgac ccgcggccgg gatatacccg
     1021 catcacccca ttcagcctcc cgccgcggca agagcccgcc ggtacaaacg gtcgggcgcc
     1081 ggtcttcatc ataaccggcg agcagggcca gtgcaagacg acgtttttgc acctggtcct
     1141 cgggctcatt gtcggggcaa atgtccggat ccggggtgtg ctcgctcccg ggcacgtgcg
     1201 ggacggccgg aggtcgggat tcaccctggt cgacctcgcc accggcgaac acgaggaact
     1261 ctcttcagtc gaccccgacc cgcggtgtga agtccacggc cgattctact tccgccccga
     1321 agggcttgca ttcggccgcc gggcgctcgc gccgccgaac ccccgggaga ccgatctgat
     1381 ggccatcgac gaggtcggac ggttcgagct ccagggcgcg gtctgggccg accagatcga
     1441 ccggctggtc tcgctttcgc atccgccgat ggtctggacc gtccggcgcc ggctcctcga
     1501 tacggtcatc gaacgctgga agatcacaaa ccccgtcgtg gtggacgtca ggagcgcaag
     1561 cgtgatggcg acggcaggcg acgtgatgga tgccgtctgg gagtggcggg aggcgcagac
     1621 ggcatctgcg gtcccggtct cccccttctc cccgggccgc atcgccgagg gtcctccgtt
     1681 gctcgcggat gcggccgccg gcgcctcccc gggcgccccg tcaatcttcc ggcggcgcta
     1741 tggcccggaa gggtgaagaa ttacaccgga tccaggccag aaaaacaacg atggcaacgg
     1801 ccgctgccga aacatcgctc ataccattct gttctcgtga acgcataagc ccggacaggc
     1861 caccatcacg cttgctgtcc ggataacccg gtgatcctga gagagaccgg gtttggcacc
     1921 cggggcaaca gtcataggag tgcccaccag aacgccgagg gggttccgtc acgccaggaa
     1981 ctgattttaa ctgttgggga tgaaaggggc ggaacgggaa tttcccgccc acaaggtgca
     2041 gggcagttca cccaaacctt tttaccaccc ccgtaaatgt tattatattt acatttccat
     2101 aaaattaaat aaaacaggta tacatattgg gtatggactg gtgtgccgga tgacccgtgg
     2161 ggaagaaccg accgtggaag tcgggcagag gcctgagggg actatcgcca tcgaactccc
     2221 gtgggttgcc tctcgtggcg gtatgccggt ctacgaggcc gtactccgga tctttccggt
     2281 cgagaaggcg atctacatcc ttccaatcgt cctcttcgtc ttttcactct ttttcgggag
     2341 gtatatgatg gacccggtgg agaccctccg aatcctcatc gtcgggtcgg caaccatctt
     2401 cctcgatact gcgtcgtccc tgctctcgac ctttgccggt catcaggttc tcctcccctc
     2461 cttcgaacag gcctgggggg catcggagca gacggtcatc ttccagatca ggctcccgcg
     2521 ggtgatagct gcaatgcttg tcgggggagg gctcgccctt gcgggagcct cgttccaggg
     2581 cctcttcaga aatcccctcg tctcccccga tatcctcggc gtggcatcag gcgccgggtt
     2641 cggggcggca ctcgggatcc tcatctccgg agacccccgg gtcatccagg tctctgcttt
     2701 catcttcggg atcgtcgcgg tcgccgtcac ctacggcatc agccgtgtct accggaactc
     2761 ctcgaccctc atcctcgtcc tcggggggat cgtcgtcggg tccgtcttct ccgcgctcct
     2821 ctccctcacc aaatacgtcg ccgatcccta cgacactctg cccgcgatcg tcttctggtt
     2881 gatggggagc ctctcggcag tttcaaacgc cgatctcctc gcagtcgctc cgccgatact
     2941 cctcgggggt ctctgcctct tccttatccg gtggcggatc aacctcctct ccgtcggcga
     3001 agaggaggct caggcactcg gggtggatac gaagaagatg accgcggcga tcatcgttgc
     3061 ctcgaccgtc gtcaccgcct ccgcggtctg catcagcggg atcatcggct gggtgggcct
     3121 cgtcgtcccc cacataggga ggatgctcgt cggccccgat ttccggaggc tcatccccgt
     3181 atcggccgta ctcggggcct cgttccttct cgtcgtcgac gacatcgcac ggaccctgac
     3241 cgcagccgag atccccctcg gggtcctgac ggcgctcatc ggcgcaccat tcttcgcata
     3301 cctcttaacg cgaaagagag ttggctggtc atgatcctga acgtcgagca tgcgtcgttc
     3361 tcgtacgacg gcatcacaaa acagtttgaa gatatctcct tctccctccg gagaggcgag
     3421 gtgctctctc tcctcgggaa gaacggtacg ggaaagtcaa ccctgatcaa atgcatcctc
     3481 aacatcctct cgctccagag cgggaaggtg gagatcaacg gccgccggat cgccgggatg
     3541 aggcccgatg aggtcgctcg agaggtcggc tacgtccccc aggtccaccg cgccgccttt
     3601 ccattcacgg cgctcgactt cgtcctcatg ggaagggccc cccatatctc gaccttctcg
     3661 gtcccgaaag aggaggacta caggaaggtg gaagaaacgt ttgcccggat cggtatcacc
     3721 catctccggg agaaacctct tccggagatg tcgggcggag agtcgcagat ggtgatgatc
     3781 gcccgcgccc tcgcccagga gccatcgctg ctcgttctcg acgaaccgac atcgcatctc
     3841 gatatcggca accagatgaa gatgatcgcg acgatcaacc ggctcgccgc cgaagggatc
     3901 ggcatcctga tgagcaccca cttcccggac cacgggttca tgacctcgca tacggtcgcc
     3961 atcctcgaga acggcaggct gatcgcgcag gggccggccg aggacgtcat cacgagagag
     4021 aacctgtaca aagcctatgg ggtcgacgtc tgcatccgct acatcgagga ggcggggcgg
     4081 atggcctgca tcccgataaa ctcgtgcggc gttcccgggt agcagggaga gctacatcgc
     4141 tcccggcacg gcccgcaggc gcacctccga cgaaccggcg gcggagatgc gcgggtgtgc
     4201 ttatgggcgg taaaacgacc tgtttccggt ccttatgctc gcctcggccc cgtaaaacgt
     4261 ccggtagaac cgatcctcct ctgcagcaag atcgaacgcc gcacacgccg ggtggagggt
     4321 gcccgcgatg gcagagaggc cgagcaccca gcgggggttg ccgaaatccc agcccggcgg
     4381 aagggtgtag acccggccgt gctcgatcgc atcggcagag agtcccatcc tccggcatgc
     4441 cgtaaggtaa tcggaggccg gtgcggagag gaacccggat atgaatatgt actccgggtt
     4501 gaacgcggcg aactcttccg gggttatgtt gacccccggc tttccggccc ggacgatcct
     4561 gcggttgacg gggtcgccgc cggccgcctc cacgaggtcc atctcgaacc ggtcggcgtt
     4621 cagggcaaaa agcggcgtcc ccatcgagta gtaaacccgg ggccggggat acccttctac
     4681 tgccgacgag accgccgcca cccggtcgcc gatataggcg gcaagcgcct cagccgcttc
     4741 ggggcggccg gaccgctccc cgagatcccg gacaaggccg gtatacccgt ctatcgtctg
     4801 gatcttcccg tgaaggtccc ggatatagtc gtcctttatg accccggtcc agatccgggc
     4861 gagatcggcg tcggactcca cgccgaggca gaccagaacg gcatggacca tctcaagcgc
     4921 cctggtgaac cggtagccgc ccatcatacc gggttcggcg tacggggtct ttccgatgct
     4981 cccgagaacg gggagcgaga acccgcaaag gcaccgggcg tccggcggaa ccgcgatcgt
     5041 ccgcgccccc atcggcccga agaactctcg cccgacgacc ctctccccac aggacgggca
     5101 gaccgtatcg aggtacccgg tcccggggga gttgaagagg tagacatacc ggagtctccg
     5161 ccgcaggtcg tcgcagatcc gctccgactc cgcgatcgtc ggttccctct ccggcgactc
     5221 gtcgccaaac ggtatgaatc gcatgacgag gagcgggata tcaggagata tatccgcaat
     5281 ccgggcggca acagcgcgga tctcgtcgtc atagccgcgg gcgtagatac aggagacctc
     5341 gacgtgaacg cctgcttcgt gaagggtctt caccgcccgg aaagccggtt ctgccgagac
     5401 cgctccgcac gcacggtagc gcgcatcgga tgctcctttc agcccgatgt ttacgaagtc
     5461 tatggcaggc gcgagatcat ggagggcttc ttccgtatag tagccgttcg tcgcgcaccc
     5521 gacgaggagc cccgcatcgc gcgccgcccg cgccaccgcg agaaacgtcc ggtgcatggc
     5581 ggtcggatcg ttgaggcaga acgctatccc gcggcacccc tcctcccggg cgcgccggat
     5641 cagggcgtcg ggcgggatat gcttgagtgc cccggagacc gcctccgcgt ggagggcgac
     5701 cgtctcggat atgcatcctc cacaggagaa ggtgcacccg atgccgctcg cctgcaggaa
     5761 cgtctcccgg ggatagtagt ggcacatcgg catcgtctcg atagcaatcg gcatgaccgc
     5821 gatgtagcgg tcgggatacc gctcgacgat cgtcccgccc tcgtttgtgt acatcctgca
     5881 ccgcccgacc cctccgggcc cgatcacaca ccggttctcg cagatggagc accgcatacg
     5941 cctatcgccc cttctccatc atgacccaga acccggagtc atcgttgacg atccggtgag
     6001 ggagcccgag gccctcgatg acgccgacaa cccgctcccg gttctccggg ccgaggtttt
     6061 tatcccgaaa gttcttccaa tcggggttct ccttcgtcat cgcggccaga actgcatccc
     6121 ggagttcggc ggtcccgaac ccgcccccga tataggtctt cccaccgggt gcgagaaccc
     6181 ggtggatctc ggagaacgcc cgggcgaggt cctcccagaa gaagatcgaa ccgcggctga
     6241 ccgcgatgtc gacggatccc gcgggaagcg ggatatcgtg gacgtcgccc gggagaaccg
     6301 agaggcggcc ggagaggccg gcctcccgga tgttcccttc ggcgagggca agcatctccg
     6361 aagacgagtc aaagagcgtg acggtaaggt cgctcgcccg cgcaagcgct atcccgagcg
     6421 atccggggcc gctgccgata tcaaggcacc gcccgcggga tatcctgcac cgctcgacga
     6481 tctgctctgc gatcaccggg tagaccggtg cgaagacggt ttttgcgatc cggttcatat
     6541 tcttcgatcc gtcccggtcg aacctgcctg gcgtgtatcc catcttctca ccctcagagg
     6601 ttcaggatct ggtggatctg gtcgtcggag agcgatacgt ggaggaagat ctcatgatac
     6661 tcccggacga ggctctcaag gtccatatcc cggaagagat cgggatacag agttttggca
     6721 gtccagggaa tcccgatgat ccggttgatg cccggcgggc ggtcaaacca gcagaacgca
     6781 gtccgcgggg tgagatagac ccgacccgtc ttcacggcgg tgatctcctg ccaggccggg
     6841 tcggtcccga ccgcggcgta gaaccccgga ttctcgacca gaatgacatc cggatcccag
     6901 gcgatgacct gctccatcga gacctccgtc attcccatcc cgggcgttat cgcacaatct
     6961 gcaatgtttc tcccgccgca gaggtcgatg agttcggagt gggacgaacc ggagggatcg
     7021 gtcgtaagtc ccctgggccc ctcggcatag tagaccccca cccgctcctc gtcggggatc
     7081 gaggccgccc gctccgccac cgtggcaagc acgccctcgt agaaggcgat catcgcttcc
     7141 gcctgctcct cctcgccgag gaggtcgccc acgaacctga tgggcgcgga gtagccggtg
     7201 gagttggtgc tatcctcgac agcgacgacc gggatgggtt ccatcttctg ctgccgctcg
     7261 gcgatggtgg cactcgccgc tccgccgtcg gtggtgaaac cctcgatgac gatgtcgggg
     7321 tgcatcgata tgaacgtctc gtagttgccg gtctctttcc cgaaccagcc cccgatgacc
     7381 gggagcgcca tgtacttctc ggcggtgtat cccttctcgg gcgcgaagtt ccagccttta
     7441 agccggtcgg gcgcgaccat gtagacgagg tttgtcgacg gcggcgaggt gcagagaaca
     7501 ctctcgatct ccgacgggac gacgacggtc cggtccacca tatcggtgat cgtgcggtct
     7561 tcggccgaag gagatgattc ccgggcggtt gtgcaggcgg cggaggcgca gaggaggacg
     7621 gcacagacac cgagcatgat gagcagaaga tgacagcgtt ttgtaataac taccacgtcc
     7681 attcagtctt ctccgatacg ctccggggag ctatcggagg cttatggtat cgtggttggg
     7741 ggcggccctt attaaaaata gtcatttaa
//
LOCUS       DAOSII010000088         7704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_8737_length_7704_cov_47.490783, whole genome shotgun sequence.
ACCESSION   DAOSII010000088 DAOSII010000000
VERSION     DAOSII010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7704
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8737_length_7704_cov_47.490783"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="PLV90_07325"
     CDS             complement(<1..167)
                     /locus_tag="PLV90_07325"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HOF96964.1"
                     /translation="MKDTAINVLKILEESPGPVSGERIAEQLGITRSAVWKQVRELRR
                     LGYGIASSRAEG"
     gene            complement(173..607)
                     /locus_tag="PLV90_07330"
     CDS             complement(173..607)
                     /locus_tag="PLV90_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2111 domain-containing protein"
                     /protein_id="HOF96965.1"
                     /translation="MDMAMEQYVISASSTARDLAPIMICIHDLLGRLPVTARSRDHPG
                     IRIEDGKIIDQAYTGPILEEVLAGNTMKRVQPPAGPYRGIPVVVAPVRDSEGRAIGAI
                     GIVDVTGIFDLAGFMEQNAGIRKQVCGQDPCPLPTESPAAKK"
     gene            complement(627..1193)
                     /locus_tag="PLV90_07335"
     CDS             complement(627..1193)
                     /locus_tag="PLV90_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96966.1"
                     /translation="MGLLWDSPLMFSRLIEDCGACCEMVNPYMLASPFWRGRFTALIV
                     PTGFANPAYSNLLPALRASSGRIRRFIKSGGRLLAFGAGCPREDAYDWLPFPVTYAFG
                     YGPRAVRFTGESRYTSLFAGYDLAAVECDGTFPAHGGETLAVSASDEALLIEKTIGSG
                     TILVSSIHEYPSREFLKGFSCGDRETLF"
     gene            complement(1202..2638)
                     /locus_tag="PLV90_07340"
     CDS             complement(1202..2638)
                     /locus_tag="PLV90_07340"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOF96967.1"
                     /translation="MLEGINKIGDLEWEVPIGYVADMRVPGRFFLSLALAETLEEGAV
                     RQLANVATLPGIVKHSLAMPDIHWGYGFPIGGVAAFDMTEGVISPGGVGFDINCGVRL
                     ITTPLTEADLAGKKRDLIERFFNAVPTGVGAKSTLRVSNKELSDVMVNGARWAVERGF
                     GIEEDLVRCEEEGAMPGADPGAVSAKARQRGLPQLGTLGAGNHFLEIQVVKEVVDPEA
                     ARAFGIAEGQICFMVHCGSRGLGHQVATDHLRILESALGKYRIRLPDRQLACAPVDSP
                     EGRAYYGAMVCAANYAWANRQVIMHEARKVFSGMFGIDYDDMRLIYDVAHNVAKFERH
                     DIDGASAEVCVHRKGATRAFGPGAPGIPREYAAVGQPVLIPGSMGTSSYLLRGTTTAM
                     QKTWGSTCHGAGRVLSRSKAKKEVRGRELREQLAGEGILVRAPHDAVLAEEAPEVYKP
                     SHEVVRVVHEAGLSGIVARLNPLGVIKG"
     gene            complement(2648..3055)
                     /locus_tag="PLV90_07345"
     CDS             complement(2648..3055)
                     /locus_tag="PLV90_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOF96968.1"
                     /translation="MSFEELDHTADVLMRVRGATITEVFSDAGRAMFHVMYGSCEDRG
                     VKRRFALEAEDLESLLIDYLSELLFITDVENIVFCSFDIDIEGTRLSAVARGEPFDPA
                     RHAAGALIKGISYFGLEIVKEEETYAVDIIFDI"
     gene            complement(3059..3328)
                     /locus_tag="PLV90_07350"
     CDS             complement(3059..3328)
                     /locus_tag="PLV90_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96969.1"
                     /translation="MQSDTQEPGQSRGIYVTDDVRSYILQRGRDFRVCTSCGGPILLP
                     ITVKPPKATDLQVPVGDHTIYISKYQARYVDVIRRDMIPIFFEDF"
     gene            complement(3380..3694)
                     /locus_tag="PLV90_07355"
     CDS             complement(3380..3694)
                     /locus_tag="PLV90_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2551 domain-containing protein"
                     /protein_id="HOF96970.1"
                     /translation="MRSPYELKSEIEARLKGYLSRDRDGIRRELLSLFVRVKSLTIPQ
                     IYMKLQEQFSISYHSVASMVGIIASRIGILHVRRNADGTNTVYELKDQYVDVVANILG
                     TT"
     assembly_gap    3748..3847
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3848..4700
                     /locus_tag="PLV90_07360"
     CDS             <3848..4700
                     /locus_tag="PLV90_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOF96971.1"
                     /translation="SVRYDINKYSPRQMVALPLVLLLLAGIVLGYTTLTTGLPLKPGI
                     DFAGGTAVTAFTSDSRETIEATFAGYPLLSVGEGIGGGKYIQFGPMDDARYQSLVALI
                     NEKYPDAKVDQIGETFGKTLQGQAFLALIFSFVGMSVVVLIAFRNLVPAGAVVLAAFA
                     DIAVTAAIMQVIGIPLSLGTTAALLMLIGYSVDSDILLTTRLLKRKGKLDEKLAGAFR
                     TGIIMTTTTLAAIAAMWVVSTAGQVQIISDIASVLLIGLFVDLMNTWMLNAGILKGYI
                     LKGGGKK"
     gene            4697..6157
                     /locus_tag="PLV90_07365"
     CDS             4697..6157
                     /locus_tag="PLV90_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071806 - protein transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecD"
                     /protein_id="HOF96972.1"
                     /translation="MKRETIVHLIKDWRVALLLLLVAGSLIGIYLTPPNPANGLEGNL
                     QFGLDLQGGSWLQMEFQSVVVAYSTDKPVGDLIENLQKSLEVDVVQIDQNHLEIRKSV
                     SRADLEPLFAASDAKIVTYHEGVSTFTADEVKRILNDKVNALGMQDARINLLTPTGSE
                     YPQYVRIELAGVDMATAQEIVGKQGMFEIRIQTSGNETEHVLFGDQITSVGVPQRDTR
                     SQAWGVGFTLSDAGADAFRDAALSHGAIDNPNAHNLVMLLDNKTVYSAPLSGDLAAQL
                     RSAPVRSLSASTGTGDAGLEAAMTLEIHLRAGALPVKVDVVGSGSVPAALGEHFKTTV
                     AIAGLFALLTVGVVIYYRYREPSIVIPMLAINFSEIIILLGIARFIQQLDLAAIAGLI
                     AVVGTGIDQLVIITDEVLHEGRVPSPSLYMKRYGRAFGIIAVSAATVMFAMLPLALMD
                     LSTLRGFAIITILGVLIGILVTRPAYGKIIMEILSK"
     gene            6219..6506
                     /locus_tag="PLV90_07370"
     CDS             6219..6506
                     /locus_tag="PLV90_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOF96973.1"
                     /translation="MGKPVLMDFFAEWCGPCHQQTPIIDELKKRMGDQVDIRKIDVGV
                     DSEQTRQYAAKYDIQFVPTLVIEKDGVLVRKLVGVQDLKSLESILKPLVGQ"
     gene            6503..7153
                     /gene="npdG"
                     /locus_tag="PLV90_07375"
     CDS             6503..7153
                     /gene="npdG"
                     /locus_tag="PLV90_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HOF96974.1"
                     /translation="MKIGIVGGTGDIGEGMALRLSSKYDVIVGSREEIKALATCETCR
                     ETLQVRGLKCSLAGVTNQHAVDEADIVVLAIPFKHVAPTLKTLTGFEGKIVVTPVNPI
                     ERTAYFYYAPPPEGSAAMMIKGMLPSSATVCAAFNNIAANKWQMLDEELDYSVAVCCD
                     DEGAKQTVMELVDSVSSLRAYDAGPLAAAAIVESVTPLLLNIARFNRMRDVGVKFV"
BASE COUNT         1508 a         2279 c         2203 g         1614 t
ORIGIN      
        1 ccctccgcgc gggacgatgc gatgccgtat cccaaccgtc ggagttcccg cacctgtttc
       61 cagacggccg atcgggtgat tccgagctgc tctgcgatcc gctcgcccga taccgggccg
      121 ggagattctt cgagaatctt cagtacgttg attgccgtat ccttcatgcg catcactttt
      181 tcgctgcagg agactcagtc gggagcgggc aggggtcctg gccgcacacc tgcttccgga
      241 tacccgcgtt ctgctccata aagcccgcca gatcgaagat gccggtcaca tcgacgatgc
      301 cgatcgcacc gatggctctg ccctcgctgt ctctcaccgg cgctaccacc accgggatgc
      361 cccggtaagg gcctgcgggg ggctggaccc gcttcatcgt gtttccagcc aggacttcct
      421 cgaggatggg gcccgtgtag gcctggtcga tgatcttccc atcctcgatc ctgattcccg
      481 ggtggtcgcg ggaccgcgcc gtgacgggca gacggccgag cagatcatgg atgcatatca
      541 tgatgggagc gagatcgcgc gcagtagagg atgcagagat gacgtattgc tccatggcca
      601 tatccatact tttcatcatg ttgtagctag aataaggttt ccctatcgcc gcaggagaaa
      661 cctttgagga attcacgcga tgggtattca tggatgctgg aaaccagtat cgtgccgctg
      721 ccgatcgttt tttcgatgag aagcgcctcg tcggacgctg agacggcaag ggtctcgccg
      781 ccgtgggccg ggaatgtccc gtcacactcg acggcggcaa ggtcgtaccc ggcgaagagg
      841 gacgtgtacc tgctctctcc ggtgaaccgg acggcacgcg gcccgtagcc gaacgcgtag
      901 gtcaccggga acggaagcca gtcgtaggca tcctcccgcg ggcaccccgc gccgaaggcg
      961 agcaatcgcc ccccgctctt gatgaaacgc cggatacgtc cactggaggc ccggagtgcc
     1021 gggaggaggt ttgagtaggc cgggtttgca aacccggtcg gtacgatgag cgcagtaaat
     1081 cgtccccgcc agaaggggga ggcgagcatg taggggttga ccatctcgca acaggcgccg
     1141 caatcttcga taagccgcga gaacatgagg gggctatccc agagcagccc catcctgccg
     1201 gtcacccctt gatcaccccg agcgggttta gccgggcgac gatacccgac aggcccgcct
     1261 catgtacgac gcgcaccacc tcatggctcg gtttgtacac ctcgggcgcc tcctccgcga
     1321 ggacggcgtc gtgaggagca cgcacgagga taccttctcc tgccagttgc tccctgagtt
     1381 ctctccccct gacttctttc ttcgccttcg atcggctgag cactctcccc gcgccgtggc
     1441 aggtgctccc ccaggtcttc tgcatggcgg tcgtcgtgcc gcggaggagg taggaagacg
     1501 tccccatgct ccccgggatg aggaccggtt gcccgaccgc ggcgtactcg cgagggatgc
     1561 cgggagcacc cggcccgaat gcccgtgtcg cgcctttacg gtggacgcag acctccgccg
     1621 acgcaccgtc gatgtcatgg cgctcgaact tggcaacgtt gtgcgcgacg tcgtagatga
     1681 ggcgcatatc gtcgtagtcg atcccgaaca ttcccgaaaa gacctttctg gcctcgtgca
     1741 tgatgacctg ccggttggcc caggcgtagt ttgctgcaca gaccatcgca ccgtagtaag
     1801 cgcggccttc aggggagtcg accggggcgc aggcgagctg ccgatcgggg agacgtatcc
     1861 ggtactttcc gagcgcgctc tcgagtatcc gcaaatgatc ggtggcgacc tggtggccaa
     1921 gaccccgtga gccgcagtgg accatgaaac agatctggcc ttcggcgata ccgaaggcgc
     1981 gcgccgcctc cgggtcgacg acctccttca ccacctggat ctcgaggaag tggttgccgg
     2041 cgccgagcgt cccgagctgc ggcaaacctc tctggcgcgc tttggcgctg accgcacccg
     2101 gatccgcgcc cggcatcgcg ccttcttcct cgcaccggac gagatcctcc tcgatgccga
     2161 acccgcgctc gaccgcccac cgcgcaccgt tcaccatgac atcggagagc tccttgtttg
     2221 agacccggag agtgctcttt gcacccacgc ccgtcggcac ggcgttaaag aatctctcga
     2281 tcaggtctcg ttttttgccg gcgaggtcgg cctcggtgag gggcgtcgtg atcaggcgga
     2341 cgccgcagtt gatgtcgaat ccgactcccc cgggagatat gacgccttcg gtcatgtcaa
     2401 acgccgcgac accaccgatg ggaaatccgt atccccagtg gatgtcgggc atggcgagcg
     2461 agtgtttcac aatcccgggc agggttgcga cgttcgcgag ctggcggacg gccccttcct
     2521 caagcgtctc cgcgagggcc aaagagagga aaaaacgtcc cggcacccgc atgtcggcga
     2581 cgtatccgat ggggacctcc cactcgaggt ctccgatctt attgattcct tcaagcatgt
     2641 cgatccctca tatatcgaat atgatatcta cagcataggt ctcttcttct ttaacgattt
     2701 cgagcccgaa gtaagatatc cctttgatga gcgcccctgc ggcgtgccgc gcggggtcga
     2761 acggttcgcc ccttgcaaca gcagagagcc gggtgccttc tatatcgata tcgaaggagc
     2821 agaagacgat gttctcgacg tcggtgatga agagcagttc cgagaggtag tcgatgagga
     2881 gagattcgag atcttccgcc tcaagggcga accgtcgttt tacaccccgg tcctcgcagg
     2941 agccgtacat cacgtggaac atggcccgac ctgcatcgga gaagacctcc gttatggttg
     3001 cgccccgtac ccgcatgagg acgtcggcgg tatggtccag ttcttcaaaa ctcatggcct
     3061 aaaaatcttc aaagaatata gggatcatat cccggcggat gacatcgacg tagcgtgcct
     3121 ggtacttcga tatgtagatg gtgtggtcgc cgacaggaac ctgcaggtcg gtcgctttgg
     3181 gcggtttcac cgttatcggc aggaggatcg ggccgccaca ggatgtgcag accctgaaat
     3241 cacgtcctct ctgaagtatg tatgaccgga catcgtctgt aacgtatatg ccccgtgact
     3301 gtccaggctc ctgtgtatcg ctctgcatga tagtctttta tgttggtctt cggtgtaaaa
     3361 aaagagtctg ttttggggtt tacgtagtcc cgaggatatt cgctactaca tccacatact
     3421 ggtcttttaa ctcataaacg gtgtttgttc cgtctgcgtt ccgtctcaca tgcaggatgc
     3481 ctatccggga cgcgatgatg cctaccatgg atgcgacgga gtggtaactg atggagaact
     3541 gctcctgcaa cttcatatag atctgaggta tggtcagtga tttcactctt acgaagaggc
     3601 tgagcagttc acgccggatt ccgtcccggt cccgtgagag ataacctttg agtcgagctt
     3661 ctatctcgct tttgagctca tagggtgatc tcatatgtgc ttgtgattcg gtattttata
     3721 tatatctttt cttttcattt atttaatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnatc tgtcagatat gacatcaaca aatactctcc gcggcagatg gtagcgctgc
     3901 ccctcgttct tctcctcctc gccgggatcg tgcttggcta caccacgctc accaccggcc
     3961 tgcccttgaa accgggcatc gactttgcgg gaggaacggc ggtcaccgca ttcacctctg
     4021 acagcagaga gacaatagaa gcgacttttg ccgggtatcc gctcctatcg gtgggtgagg
     4081 gtatcggcgg cgggaagtat atccagttcg gccccatgga cgacgcccga taccagagcc
     4141 ttgttgcgct catcaacgag aagtatcccg atgcgaaggt ggatcagata ggggagacgt
     4201 ttggaaagac gctccagggt caggccttcc ttgccctgat cttctcgttc gtcggtatgt
     4261 ccgtcgtggt tctcatcgcg ttccggaatc ttgtgcccgc gggcgcggtc gtccttgcgg
     4321 cctttgccga tatcgccgtc accgccgcga tcatgcaggt catcggcatc ccgctctccc
     4381 tcgggacgac ggcggccctg ttgatgctga tcggctactc cgtcgacagc gacatcttgc
     4441 tgaccacgcg cctcttaaag cgcaaaggaa aactcgacga gaaactcgcc ggagccttca
     4501 ggaccggtat catcatgacg accacgacgc ttgcggccat cgccgccatg tgggtcgtct
     4561 ccacggcagg gcaggtccag atcatcagtg atatcgcaag cgtcctcctc atcggtctct
     4621 tcgtcgacct gatgaacacc tggatgctga acgccgggat cctcaaggga tacatcctca
     4681 aagggggcgg taagaaatga aacgcgagac catcgtacac ctcatcaaag actggcgggt
     4741 tgccctgctc cttctgctgg tcgccggctc cctcataggc atctacctta cccccccgaa
     4801 tccggcgaac gggcttgaag gaaacctcca gttcggcctc gacctccagg gcggttcatg
     4861 gctgcagatg gagttccaat ccgttgtcgt agcctattcg accgataagc cggtcgggga
     4921 tctcatcgag aacctccaga agagccttga ggtggatgtc gtccagatcg accagaacca
     4981 tctcgagatc aggaagagcg tctcgcgcgc cgatctcgaa ccgctctttg cagcatcgga
     5041 tgcgaagatc gtcacgtatc acgagggcgt atcgacgttc accgcagacg aagtgaaacg
     5101 gatcttaaac gacaaggtga acgccctcgg catgcaggac gcgcggatca acctcctcac
     5161 cccgacggga agcgagtacc cgcagtacgt gcggatagaa ctcgccggtg tggatatggc
     5221 gacggcgcag gagatcgtcg gtaaacaggg catgttcgag atccggatcc agacgagcgg
     5281 gaacgagacc gagcacgtcc tctttggcga ccagatcacc agcgtcggtg tgccgcagag
     5341 ggataccagg agccaggcgt ggggtgtcgg gttcacgctc tccgatgctg gcgcagatgc
     5401 cttccgggat gctgccctca gtcacggggc gatcgacaac ccgaatgctc acaacctggt
     5461 gatgctcctc gataacaaga cggtctacag cgccccgctc tccggcgatc ttgcggcaca
     5521 gctccggtcg gccccggtca ggtcgctctc cgcgagcacc gggaccggcg atgcagggct
     5581 tgaggctgcg atgacgcttg agatccacct gcgcgccggc gccctgccgg tgaaggtgga
     5641 tgtcgtcggg tccgggtcgg ttccggcggc actcggcgaa cacttcaaga cgacggtcgc
     5701 catcgccggg ctgtttgcgc tcctcaccgt aggggtcgtc atctactacc gctaccgcga
     5761 gccttcgatc gtgatcccga tgctcgccat caacttctcc gagatcatca tcctgctcgg
     5821 catcgcacgg ttcatccagc agctcgatct cgccgcaatc gccggcctga ttgcggtggt
     5881 gggcaccggc atcgaccagc tcgtcatcat aacggacgag gttctccacg aagggcgtgt
     5941 cccgtcgccg agcctttaca tgaagcgcta cggccgtgcg ttcggtatca tcgccgtctc
     6001 ggcggctacg gtcatgttcg ccatgctccc gctcgcgttg atggatctct ccaccctcag
     6061 aggctttgcc atcatcacca tactcggcgt cctgatcggt attttggtca cgaggccggc
     6121 atacgggaag ataatcatgg agatcctctc aaagtaaatt tttttcgaaa acctttattg
     6181 ccccgttcac acacaatgtg tgaatggggt atagtcttat gggaaaaccg gttttgatgg
     6241 acttctttgc cgagtggtgc ggtccgtgcc accagcagac accgatcatc gatgagctca
     6301 agaagagaat gggcgaccag gtcgatatca gaaagatcga cgtcggcgtc gattccgaac
     6361 agacgcgaca gtacgctgcg aaatatgata tccagttcgt acctaccctc gtcatagaga
     6421 aagatggggt tctcgtccgg aaactggtgg gggtccagga tctcaagagc ctcgagagca
     6481 tattaaagcc gctggtgggg cagtgaagat cggcatcgtc ggtgggaccg gtgatatcgg
     6541 agagggcatg gcgcttcggc tctcttcgaa gtacgatgtg atcgtgggat cacgcgagga
     6601 gataaaggcg cttgcaacct gcgagacctg ccgggagacc ctgcaggtgc ggggactgaa
     6661 gtgcagcctt gccggcgtca ccaaccagca tgcggtcgat gaggcggata tcgtcgtcct
     6721 tgcgataccg ttcaagcacg tggcccccac cctgaagacc ctgaccgggt ttgagggtaa
     6781 gatcgtcgtc acccccgtca acccgatcga gcgcacggcc tacttttact acgctcctcc
     6841 ccccgagggc tccgctgcga tgatgatcaa agggatgctt ccttcgagcg caaccgtctg
     6901 tgccgcgttc aacaacatcg ccgcaaacaa gtggcagatg ctcgacgagg aactggacta
     6961 ctccgtcgcc gtctgctgcg atgacgaggg agcgaaacag accgtgatgg agctcgtcga
     7021 cagcgtatcg agtcttcgtg cttacgatgc gggccccctt gcggctgcag caatcgtcga
     7081 aagcgtcacg cccctcctcc tcaacatagc ccggttcaac aggatgcggg atgtcggcgt
     7141 taagttcgtg taaaattacg ttattttaca aaattcatgc ggaagagcac gggatcctcc
     7201 ttccaggccg gtcttccggt ccggggagat gccgctttct ccatctcctc tcctcttcat
     7261 gcagagatgc gagtgaccgc cggcagaact cggtgtctgc tgttttacat ccgcctcgca
     7321 cgaggttgtg ccggcgtgcc atgcgttccc ggcgtgaaag gccacagaga tgttagtgag
     7381 tacattcgcc tgagcggagt caagaccttt tcccatttac tttttggatg tcatactgtc
     7441 gcagtaaaag ccattgattg agggttatgg cgagaatggt gagaaacatg atcaaaggga
     7501 caatgaacca ttgctgttct ggataataca ggctgcgcca gtattcaacc ccctcagcga
     7561 taacgacgat aattgctgtt cctattgtca ttctcgctaa caaacccacc atacgaccac
     7621 cacatcaact cgatctttcc gtacgttatt aatccttgca cgagtcgtct cccccatggc
     7681 gatagccacc acgatccggt gcac
//
LOCUS       DAOSII010000089         7649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_8819_length_7649_cov_46.830787, whole genome shotgun sequence.
ACCESSION   DAOSII010000089 DAOSII010000000
VERSION     DAOSII010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8819_length_7649_cov_46.830787"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            393..893
                     /locus_tag="PLV90_07380"
     CDS             393..893
                     /locus_tag="PLV90_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96975.1"
                     /translation="MSGKKLPELPEELEEFLDKPYGGLFGNTVLAQVVEEIVADPTMD
                     YRPRYLEEMTGKKAPSIREALRVLVRLGLIENVSSDVQHPVYRVVVESKKFIALSLLA
                     YAVIDDRDGTDCMNTAILDYYNRVLRAKAEPLAVATNHAGEWTVGDDCSAREAESGYS
                     LYSVGV"
     gene            896..1267
                     /locus_tag="PLV90_07385"
     CDS             896..1267
                     /locus_tag="PLV90_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOF96976.1"
                     /translation="MGEEQNERAATVNLRKLYRIEPEELQLDITHSSYSNLAYVQVTH
                     RDVCIDFLEMPGARRDGKALINGTRIYMSHAAAQKLAEALSGILERVHTEEGMEKYVP
                     GGLEGRAPSTKAGRKREDRPV"
     gene            complement(1436..1762)
                     /locus_tag="PLV90_07390"
     CDS             complement(1436..1762)
                     /locus_tag="PLV90_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOF96977.1"
                     /translation="MEYSRIAMHPLFQIRQNLCVIRERYGVSRIGLFGSVARGEETPA
                     SDIDVLVEFREGEETFDHFMDLKFYLEDLLGRKTDLVIADTLKPRIRDAVLGEVVYAW
                     RPPVVS"
     gene            1987..2163
                     /locus_tag="PLV90_07395"
     CDS             1987..2163
                     /locus_tag="PLV90_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96978.1"
                     /translation="MRSEFKAELNISAVRMPDLHININVTPILQGWKTIDYEKRGRED
                     VLKDWETRQQRENY"
     gene            complement(2382..2771)
                     /locus_tag="PLV90_07400"
     CDS             complement(2382..2771)
                     /locus_tag="PLV90_07400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOF96979.1"
                     /translation="MEEFTVEEVIDYHVEILLASDREEDRGLEGRLLTPGNLDFVIEF
                     SNGFSDPFERAAFILHGLATGHAFAEGNKRIAFLLASLVLLRTPERYEIQSCPEENDR
                     LVRDVAEGKKTREEVETWLRSVTKGDY"
     gene            complement(2892..3179)
                     /locus_tag="PLV90_07405"
     CDS             complement(2892..3179)
                     /locus_tag="PLV90_07405"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOF96980.1"
                     /translation="MSGASTKGVAAGAIWPRSVPIERRVPSDVPAVVLRFIAIGEATK
                     QIPVGIIVRRPEIEWQKIAGIRDISVHSLDAVKPTVSWDIIQTTLLVQTRR"
     gene            3369..4127
                     /locus_tag="PLV90_07410"
     CDS             3369..4127
                     /locus_tag="PLV90_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HOF96981.1"
                     /translation="MTIEILKSIARNGQVFTLEQLHRQTEIPKSVLSVMLSRWEERGF
                     VERIEKGKYLIIPLASEKGKYTLHEFVIASHLVRPSAIAYWSALHYHGLTEQIPTTVF
                     VQTTARKKSNQIEVFGVNYRIVRVKPEKFFGFAKEWIEETQITVTDREKTVIDCLDRP
                     EYAGGIVEVAKALENASLDRENLNRYARMIGNNAVVRRLGYLCEQMGVPLDLPLPTSR
                     NYLLLDPAMPRQGEIDPKWRLVINTEITRQGDSG"
     gene            4136..4333
                     /locus_tag="PLV90_07415"
     CDS             4136..4333
                     /locus_tag="PLV90_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96982.1"
                     /translation="MEIKEVIPDGSVLAGKRERFSASWNASFRHQMKAIPDFEEAFER
                     ALGCVELYARRSGEAPQDRGD"
     gene            complement(4466..5434)
                     /locus_tag="PLV90_07420"
     CDS             complement(4466..5434)
                     /locus_tag="PLV90_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOF96983.1"
                     /translation="MFEKTIFVEGINGGVQVDAPFVVDVAELREKKGGKYIQLAISDR
                     TGRGVCKVWGTPGQSAEQIEAFGLAIRAGEVYRIRGYAKVYNGTCEINVNDGIVDLLA
                     PVPTDMFDPSLFVYAPVDLDDVREGLGRMAAKIVDPGIYSLVSRVMGSTPAFLEAPAA
                     KRRHHAYIGGLAEHTLETAEIALSLAGTVSRARMDTDVLLAGALLHDVGKAACFRRQG
                     FSFVALPDYTLVGHTALGAAAILRQSAGVDPVRFSHILHILMSHHGPHGEVQPRTPEA
                     WAVHFADNASATLRAACDDTADLVPGEERRGARCGGPVYRFSTPTE"
     gene            complement(5753..6460)
                     /locus_tag="PLV90_07425"
     CDS             complement(5753..6460)
                     /locus_tag="PLV90_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOF96984.1"
                     /translation="MLTDTEDREAADCTLVIPAYNEEKRIQSLLKDLSDFRGEIIFVC
                     DGTDATPAFIAAFAADHPSLSIRCLTFSARLGKGGGVVAGLREASTRYVGYMDADGST
                     SLAEMERLFDRLADVDGAIGSRWLPGSVMTVRQGLRRRAESRLFNLAVRLLFGLDYRD
                     TQCGAKVFRREALEAVLPSIRSTGFEFDVELLWRLAQSGCRVEEVPITWENRDESKVG
                     TADAKEMLSGMIRLRFG"
     gene            complement(6454..6969)
                     /locus_tag="PLV90_07430"
     CDS             complement(6454..6969)
                     /locus_tag="PLV90_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOF96985.1"
                     /translation="MKLLLAVAPERFRDEELEVPKRAFEEAGIGIDIASTVAGTCTGM
                     LGARTEAAMTFDEVDPGDYVGIVVVGGAGSADHLWGSRRLQELVRTFFEEGRVVAAIC
                     LAPVVLARAGILAGRQATVYPAPAAVAEMKRAKAVLHDIPVVADMQIVTANGPAAAAQ
                     FADTVITKLEC"
     gene            7286..>7649
                     /locus_tag="PLV90_07435"
     CDS             7286..>7649
                     /locus_tag="PLV90_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF96986.1"
                     /translation="MQRSTRIGITLLSALALIAAAWAGAGEETIIWSRTYGGPDADDT
                     AYAVVPGQEGFLLAGVTASSGEGKTDAWVIGLTAEGRPEWNRTYGGKEADTARAIART
                     DDGGLLFAGNLTYVTNGTQ"
BASE COUNT         1653 a         2307 c         2299 g         1390 t
ORIGIN      
        1 cggggtggtg tgtctcctgg ggtggacggc gatcggaaga cgggggttgg taacattgtc
       61 aactaccccc gactaaagtc aagggcatga gcgtgggatt gccggagcgg aaggtcggaa
      121 caaggggagt atgacgccgg ccgcctcgcc cgcaccggca gtctcaaacg acgatggttc
      181 gtaaagatga aacttgaaaa gctcgtgtgg gcagaccaat ggatgaccta ctacgacctc
      241 gaagtgcccc accaccggca ctggaccgaa ctgaagaaat aaattctata tcagaacatt
      301 tttttactac cactcactac tacttactac ccgtgagagc acccgtagcg ctcttgatag
      361 gagcacgggt gctccagttg gaggggtaat ttatgtcagg gaagaaactg ccggagctcc
      421 cggaagaact cgaagagttc ctggacaaac cgtatggcgg cttattcggg aacaccgttc
      481 ttgctcaggt cgtcgaagag atcgtcgccg atccgacgat ggactaccga ccccggtacc
      541 ttgaggagat gacgggcaaa aaggccccca gcatcaggga ggcgctcagg gtgctcgtca
      601 gactcgggct gatcgagaac gtatcaagcg acgtacagca tcccgtctac cgggtcgtcg
      661 ttgagtcgaa gaagttcatt gcgctctccc tgctggcata tgccgtaatc gacgatcggg
      721 acgggacgga ctgcatgaac acggcaatcc tggactacta caacagggtg ctcagggcaa
      781 aggcagaacc gcttgcagtc gcaacgaacc atgccggcga gtggacggtc ggagacgatt
      841 gctctgcacg ggaggcagag tcagggtatt cgctctactc ggtgggggtg taaccatggg
      901 agaggagcag aacgagagag cagccaccgt caacctccgc aaactctacc gaatcgagcc
      961 tgaagagttg cagctggata tcacgcacag ttcctactca aaccttgcgt atgtgcaggt
     1021 aacgcacagg gatgtctgca tcgactttct ggagatgccc ggcgcccgcc gggacggtaa
     1081 agcgctgata aacggaacgc gtatctatat gtcgcacgct gccgcacaga aactcgctga
     1141 agcactaagc gggattctcg agagggtgca taccgaggaa ggcatggaga agtacgttcc
     1201 cgggggtctg gaagggagag caccctcaac aaaagcggga cggaagcggg aagataggcc
     1261 cgtatagagt tgccgattga ttgcgttcca tactcgatcg cgtagaatct accatcagac
     1321 aaacattcgt ttacataaaa gcagcccgaa gagtcacacg aaagtacgga gaggctccca
     1381 cgaactcctc aaacgtcgga catatcggat cgatgcggca gcgtcgcgga tatcctcaag
     1441 atacaacagg aggtctccag gcatagacga cctcaccgag gacggcatcc cggatccggg
     1501 gcttcagggt gtcggcaata acaaggtccg tcttccgccc caggagatct tcaagataga
     1561 acttgaggtc cataaagtgg tcgaaagtct cctccccctc ccggaactcc accaggacat
     1621 cgatgtcgct tgccggcgtc tcctcgcccc gtgccaccga cccgaagagg ccgatacggg
     1681 acacgccata gcgctcccgg ataacacaga ggttctgccg gatctggaag aggggatgca
     1741 tcgctattct tgagtactcc atccattgtc ttataccctc cgatagccgg aggcactccg
     1801 ctcacgcagc tccggttccc gggggacgct gtcgggacgc tactccgccc acgagatcac
     1861 ccggttgctc gggctcggag tggatgtagc cagcgagatg cttacacacc tcctggaggc
     1921 cggccaggag catcgagagg agcgggattg cccatgatcc ccccgacccc ggtggagaac
     1981 gtccacatga gaagcgagtt taaggcagag cttaacataa gcgctgtgcg tatgccggac
     2041 ctgcacatta atatcaatgt gacaccaatt ctacaaggct ggaagacaat tgattatgaa
     2101 aaaagaggta gggaagatgt actcaaggat tgggaaacca gacagcagcg agagaattat
     2161 taagtccgtg cgcccatgcc gcttccgctc ctctggaaat ttaatcgctt caacgcagag
     2221 ttctggttcg ttcagctcaa tcggcacgag taccattgca tctccctctc agtgccctcc
     2281 gccagaggcg tgggagaagg cgccacagcc ttcaaaacag gaagacttca gaatgcttct
     2341 ggggaagcac aagaagaccc ttgagcttct ggcaaaatag atcaataatc cccttttgta
     2401 acagagcgta gccaggtctc gacttcttct ctcgtttttt tgccttctgc aacatctctt
     2461 acgaggcgat cattctcttc ggggcaactc tggatctcgt atcgttcggg ggttcggagc
     2521 aggacgagcg aggcgagcag aaacgctatc cgtttatttc cctcggcgaa ggcatgccct
     2581 gtcgcaagac catgcagaat gaaagctgct ctctcaaacg gatcagagaa accgttgctg
     2641 aactcaatga caaagtcgag atttccggga gtgagcaatc gaccttccag tccccggtct
     2701 tcctcgcgat cgctcgccag gaggatctcg acatggtaat cgataacttc ctcgaccgtg
     2761 aactcttcca tccggttctc ctacctaccg tccctatcca gcgttgcgcc ggaaagaata
     2821 gtgaaatgtt aacgatactc gcgaaaaaag gtttttgatc ggtataccgc gttcctccga
     2881 gaaagaccgc gtcaccgtcg cgtctgcacg agcaatgtag tctggatgat gtcccaggag
     2941 accgtcggtt tcaccgcatc gagggagtga acgctgatat cacggatccc ggcaatcttt
     3001 tgccactcga tctccgggcg gcggacgatg atgccgaccg gaatctgttt cgtcgcctcc
     3061 ccgatggcga taaaccggag cacgacggca ggcacgtctg atggaacccg gcgctcaatg
     3121 gggacgctcc tcggccatat cgctcccgca gccacaccct tcgtactcgc tccgctcatt
     3181 gccgggtttc tgctcgggaa acctccccat caccaggctc cccgtcctta ccgtgacgcc
     3241 gccgatgtaa ggcggcgggg cgctcctgat cagggagatg gccggcgagt gtgatcactt
     3301 taatgtacac caagattatc aattttgata aggttggtgt actaaataag gaacgagaga
     3361 ggcaacacgt gacgatcgag atcctgaagt ccatcgcccg caacggacag gtattcaccc
     3421 tcgagcaact gcacaggcag acagagatcc cgaaaagcgt gctttccgtg atgctctccc
     3481 ggtgggaaga gcggggcttc gtcgagcgga ttgagaaggg aaaatacctg atcatcccgc
     3541 tcgcaagcga gaagggcaag tatacgctcc acgagttcgt gattgcgtcg cacctcgtcc
     3601 gaccatccgc gattgcatac tggagcgcgc tccattacca cggcctgaca gaacagatcc
     3661 cgacaacggt cttcgtccag acgacggcgc ggaagaagag caaccagatc gaggtgttcg
     3721 gcgtgaacta ccggatcgtg cgggtaaagc cggagaagtt cttcggcttt gcgaaggaat
     3781 ggatcgagga gacgcagatc accgtcaccg acagggagaa gacggtgatc gactgcctcg
     3841 atagacccga atacgcagga gggatcgtcg aggttgcaaa ggcactcgag aacgcatcgc
     3901 tggaccgcga gaacctgaac cggtatgccc ggatgatcgg gaacaacgcc gtcgtcagga
     3961 gactcggata tctctgcgaa cagatgggag ttccgctgga tctgcctctc ccgacatcca
     4021 ggaattacct cctcctcgat cctgcgatgc cgcgccaggg agagatcgat ccgaagtggc
     4081 gactggtcat caataccgag atcacacggc agggggactc cgggtgatac cgatcgtgga
     4141 gatcaaggag gtcattccgg acggttccgt gctcgcgggg aaacgcgaac ggttctcagc
     4201 atcgtggaac gcatccttca gacatcagat gaaagcgatc ccggatttcg aggaggcatt
     4261 cgaacgggcg ctgggttgtg tcgagctata tgctcgacga tccggtgagg ctccgcagga
     4321 tcggggtgat tgaggtgcga cagagttatg caatcgcgat catctacagc gacgaggacg
     4381 aggggttcat cgccgtcgtc ccggagcgca ccggttgctc atacccggca tccccctgct
     4441 cgtcgtcttc ttcgcctcta caacatcatt ccgtaggagt cgaaaaccgg tagaccggtc
     4501 ccccgcaccg cgccccccgc ctctcttctc ccggcacaag gtccgcagta tcgtcgcagg
     4561 ccgcccggag ggtggcgctc gcgttgtcgg cgaagtggac cgcccaggcc tcgggcgtcc
     4621 ggggctggac ctcgccgtgg gggccgtggt gcgacatcag gatatggagg atatggctga
     4681 accgcacggg gtcgacgccg gcggattgcc ggaggatggc cgccgccccg agcgcggtgt
     4741 gcccgacgag ggtgtaatcg ggaagtgcga cgaaagagaa accctggcgg cggaaacagg
     4801 ccgccttccc gacgtcgtgg aggagggctc cagcgaggag aacgtcggta tccatccggg
     4861 cccggctgac ggtgcccgcg agggagagcg caatctccgc ggtctcgagg gtatgctccg
     4921 cgaggccgcc gatataggca tggtgccggc gttttgccgc cggggcctca aggaacgccg
     4981 gggtgctgcc catgaccctc gatacgaggg agtatatccc gggatcgacg atcttcgcgg
     5041 ccatgcgccc gagcccttcc cggacatcgt cgaggtcgac gggcgcatag acgaagagcg
     5101 acgggtcgaa catatcggtc ggcaccgggg ccaggaggtc gacgatcccg tcgttcacgt
     5161 tgatctcgca ggtgccgttg tagaccttcg cgtaaccccg gatccggtag acctctcctg
     5221 cccggatcgc aaggccgaac gcctcgatct gctctgcgct ctgaccgggc gtcccccaga
     5281 ccttgcagac cccccggccc gtccgatcgg atatggcgag ctggatatac ttgcctccct
     5341 tcttctcgcg gagttctgca acatccacca caaaaggcgc gtcgacctgc accccgccgt
     5401 tgatcccctc cacgaagatc gtcttctcga acatcgaacc accgtgaaga gacaattcgt
     5461 gcgaagaacc ttaaaacccg gcgatttgtt ccggggtggc cgtgcctttg aggggcgcgg
     5521 tctgtccgcc ccacaaccgc tacctcttcg ccggatcatc ccggagaggg tatgtaatct
     5581 gtgccggcac tcattgcatg ccgcatacag tcgcgaccgt tcttccgatg agatgccgct
     5641 cgcgagcgtt ttatgcgtta cacgaagagc ctctaccagg gcgacgaccc gatcgcggcg
     5701 ggcggtttca gcgggatcat gggcgaattg gtaaaactgc cccgcgcccc cttcacccga
     5761 accgaagccg gatcatcccg gagagcatct cttttgcgtc agccgtcccc accttcgact
     5821 cgtcccggtt ctcccaggtg atcgggacct cctccacccg gcagccgctc tgcgcgagcc
     5881 gccagaggag ttcgacgtcg aactcaaacc cggtcgaccg gatggacggc agaaccgcct
     5941 caagcgcctc ccggcgaaag accttcgccc cgcactgggt atcccggtag tcgagcccga
     6001 agaggagccg gacggccagg ttgaagaggc ggctctcggc ccggcgccgg aggccctgcc
     6061 tgaccgtcat gacggagccg gggagccagc gcgaaccgat ggcgccgtcc acatccgcaa
     6121 ggcggtcaaa gagccgctcc atctccgcaa gcgacgtcga accgtccgca tccatgtagc
     6181 cgacgtaacg ggtcgaggcc tcccggaggc cggcgacgac accgcccccc ttcccgaggc
     6241 gggccgagaa cgtcaggcac cggatggaga gcgacggatg gtccgccgca aacgctgcga
     6301 tgaacgcggg ggtggcatcg gtcccgtcgc agacgaatat gatctcgccc cgaaagtccg
     6361 agaggtcttt taggagcgat tgtatcctct tctcttcgtt gtatgccggg atcaccaggg
     6421 tgcagtcggc cgcctcgcga tcctctgtat cggtcaacat tcgagtttcg tgatgacggt
     6481 atcggcgaac tgtgcggcag ccgcggggcc gtttgcggtc acgatctgca tatccgcgac
     6541 gaccgggatg tcgtggagga ccgcctttgc ccgtttcatc tccgcgacgg ccgccggggc
     6601 cgggtagacc gtggcctgcc gcccggcgag gatgcccgcc cgcgcgagga cgaccggcgc
     6661 aagacagatg gcggcgacga ccctgccctc ctcaaagaac gtccggacaa gttcctgaag
     6721 ccgtctgctc ccccagaggt ggtctgccga cccggcgccg cccacgacca cgatcccgac
     6781 atagtcgccg gggtccacct catcgaacgt catggccgcc tccgtcctgg ccccgagcat
     6841 ccccgtacac gtgccggcaa ccgttgaggc gatatcgatg ccgatccccg cctcctcgaa
     6901 ggccctcttc gggacctcca gctcctcgtc cctgaaccgt tccggtgcaa ccgcaagcaa
     6961 gagtttcatg caggtcttct actaaggttt gactcataag ggtattgatg gttgtggacg
     7021 ggaggcgcat gccggcaggg ccggggcagc aaccggacat ctcgcccctt cgccggagga
     7081 tacaactcct atcgggctgc cgccacaccc atgtttttga aacaagatta ttataataat
     7141 cagacaatta acgtgcagac cccatggtgc tgcgggagac ttcacctccc gggcgaaccc
     7201 gcgcttcacg acgatctatc gtggaccccg ggagatgccg gcacgaactg cagcacgcct
     7261 tccggggtga agacgaggaa acagaatgca acgcagcaca cggatcggga tcacgctcct
     7321 gtcggcactc gcgctcatcg ctgccgcctg ggcgggagca ggagaagaga cgatcatatg
     7381 gagccggaca tacggggggc cggatgccga tgatacggca tatgcggtcg tccccggcca
     7441 ggaggggttc ctccttgccg gggtgacggc atcgtccggg gaagggaaga cggatgcctg
     7501 ggtgatcggg ctgacggcgg agggccgccc tgagtggaac cggacctacg gcgggaagga
     7561 ggccgacacc gcgcgcgcga tcgcccggac cgacgacggc ggcctcctct tcgccggcaa
     7621 cctcacctac gtcacgaacg ggacgcagg
//
LOCUS       DAOSII010000090         7631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_8843_length_7631_cov_42.121700, whole genome shotgun sequence.
ACCESSION   DAOSII010000090 DAOSII010000000
VERSION     DAOSII010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7631
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8843_length_7631_cov_42.121700"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..609
                     /locus_tag="PLV90_07440"
     CDS             118..609
                     /locus_tag="PLV90_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOF96987.1"
                     /translation="MGSGTVVGNRVFIAENATVIGDVTLADDVNIWFGAVVRADRDTI
                     TVGSGSNIQDNAVVHTTIGFPVDIGSDVSVGHGAILHGCIIHDRVLVGMGAIVLNGAV
                     VGEGSIIGAGSVVTEGKEIPPNSLVLGVPGKVVRETTPEQRDGITRNAREYVKLAGRY
                     RRG"
     gene            602..1876
                     /locus_tag="PLV90_07445"
     CDS             602..1876
                     /locus_tag="PLV90_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOF96988.1"
                     /translation="MADVVVIGAGVAGIQAALDLADHNIHVHLVEREPTIGGHMAQLD
                     KTFPTNDCSMCILSPKMVDVARHPNVTIHTCSEVEGIEGEVGTFRVRVRKHPRYIIES
                     ECNGCGDCIQICPVEVYNRFDAGIGVRKAIYKPHAQAVPDIVIKDKDHCIECGLCYDV
                     CGREAILHEDEERVVDIEAASIVVATGYATFDAGKKAQLRYLLIPDVITSLEFERMIN
                     ASGPTGGRLKRLSNGNVPRSIAFVQCVGSRDISAGRPYCSGVCCMYAMKNAMLIREKN
                     PEIEVTVFYNDIRAYGKGYEEYYERAKDLGVRFIRGFPGEVLEGNDCLMMTAENTETG
                     EVETVHPDLVVLSVGLEPARGAEEVARMLGIPQDESGFFEVADQKLGPVLTVRPGIYV
                     AGTATAPRDIPDSVAMGEAAAMRAYLDTVRAG"
     gene            1879..2940
                     /gene="mtnA"
                     /locus_tag="PLV90_07450"
     CDS             1879..2940
                     /gene="mtnA"
                     /locus_tag="PLV90_07450"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOF96989.1"
                     /translation="MLELDDLCTIAWEEETGSIIYIDQTLLPGRCEQMRCTTVEDLAR
                     AIRRLEIRGAPALGIAGAMGVALAAARSTERSLDRFLADVGRAADLLRGTRPTAVNLA
                     WGIDRVIRKIAVAASVDEAKTVAAAEAKAVADEDELVCRRLGAFGEGLLPDDCTVLTH
                     CNAGALACRCWGTALGVIRSAVAAGKNVRVIACETRPLNQGSRLTCWELLRDGIDVTL
                     IPDSSAAYLMRKGRVDLVIVGADRITRDAVFNKVGTYMHAVTARHHGIPFYVAAPLST
                     FDLSRVESEIVVEERDRSELACSGDRQLAPDGVNVLNYAFDATPLDLVDAIVTEIGVL
                     RPPYAESFRLAGRREGVRP"
     gene            2937..3641
                     /locus_tag="PLV90_07455"
     CDS             2937..3641
                     /locus_tag="PLV90_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HOF96990.1"
                     /translation="MSILDSGAWVGLMTLIGEITFFLILGMVLAAVALAIIATASITR
                     GKFYLPRILIPGMVLLEGLVKAICKLLGLDDKDLITFFITLRNTMNTKAFAGTPVEQR
                     AVFLPQCLRSAECPAHLTPEGLKCRHCGRCTVGENVAWLEGIGYRVFIVPGSTFIKRM
                     VKKYRPKAIIGVGCLMEVKDGIDMSDRIGITAIGVVNFKDGCVETVADWAGVRDAALL
                     GIEPPSGAVDFHGPAE"
     gene            complement(3606..4016)
                     /locus_tag="PLV90_07460"
     CDS             complement(3606..4016)
                     /locus_tag="PLV90_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOF96991.1"
                     /translation="MKVYRHGNTYIAPRGSFFDGNVKIDGNFIAPPETHIWGNMVVAG
                     CLELGPYSTVGGYVEAESIVIGHDAKIKGPLRIQKDATVCDNACLHSIRAGGNIIIRP
                     GVRVGDVNSAETIYVYGKVTSERLFGRAVKVYGT"
     gene            complement(4103..4942)
                     /locus_tag="PLV90_07465"
     CDS             complement(4103..4942)
                     /locus_tag="PLV90_07465"
                     /EC_number="2.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HOF96992.1"
                     /translation="MSVSAFVRITRPHNAVVAGFTALIGYLVATGTLILPSLLLAAVV
                     ALITAAGNVINDVYDIDIDRINRPDRPIPSGAISLSGAKIYAVALFAGGIALATLTTA
                     LCLLIALANALILILYAAWLKRTPGIGNVAVAYLTASVFLFGGAFAGIEGLIQNISLA
                     AIAFLATLAREILKDAEDVDGDAAGGARTLPMIVGIRRTGLFAFACACGAVLASLLPS
                     GDWWGPFYLAAIAVVDIIILAGAARGVRCTTPDCVRESRATSILRAGMFAALAVFAVA
                     AVT"
     gene            complement(5006..5080)
                     /locus_tag="PLV90_07470"
     tRNA            complement(5006..5080)
                     /locus_tag="PLV90_07470"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5043..5045),aa:Arg,seq:cct)
     gene            5150..6265
                     /locus_tag="PLV90_07475"
     CDS             5150..6265
                     /locus_tag="PLV90_07475"
                     /EC_number="2.1.1.213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004809 - tRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(10)-N(2))-dimethyltransferase"
                     /protein_id="HOF96993.1"
                     /translation="MDLVEVTEGRTRFSVPEQDPHLQFPPGSGPVFYNARMEMNRDAT
                     VLLLSVLRPENYLDAMGASGARGLRVACEVGIPVTINDRSQRAVDLARQNIEALGLVA
                     DVTRRDANALMSERKFDAVDLDPFGTPAPFVDSAARSAERYLFVTATDTAPLCGAHLK
                     AGMRRYFSRPRNTEYHAEVGLRTLLGFVVREVIKYDRGVEPLFCYAHEHFHRLHLRLR
                     EGAAAADRSLARIGYVMQCPRCLYRTEQTGMLPEPEGCALCGADLVPVGPLWTGRIND
                     DEMLAAMQEALPRVTVGTGPRIARLLATCRQELDTSSHYDYHVIAKSLRVSPGGIGTV
                     VDRLVALGYRASRAHYSGTAIKTDAPLPVLESVISGG"
     gene            complement(6252..7367)
                     /locus_tag="PLV90_07480"
     CDS             complement(6252..7367)
                     /locus_tag="PLV90_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOF96994.1"
                     /translation="MSSVEEQIRELEDEIKNTPYNKATSKHIGRLKAKLAKIRDEAVA
                     RAMASSGGGEGYSVKKSGDATVVLVGFPSVGKSTLLNRLTGFDLSETADYAFTTVSVI
                     PGAMEHRGAKIQVLDIPGLIAGAAMGKGRGKEVIAVVRSADLILVLVDVFNEQHTDVL
                     LRELYDAGIRINRQRPDITIRKTGNGGVRLNTVGAVDLDLEEVRSILAENKIVNADVL
                     VRDGATQDDFIDAMIGNRVYVPAFIAVNKVDLVDGKTRAEIEEELTERFGEPPIMVSA
                     HSGYRIEDLKDAIFDDLGFMRVYLKPVGGPADMDEPLIIRSPATVEDVCNRLHREFAD
                     KFRYAKVWGRSAKHDAQRVGITHQLADGDVLSIVTRR"
     gene            7556..7630
                     /locus_tag="PLV90_07485"
     tRNA            7556..7630
                     /locus_tag="PLV90_07485"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7592..7594,aa:Glu,seq:ctc)
BASE COUNT         1384 a         2378 c         2498 g         1371 t
ORIGIN      
        1 gcgatagcag tccgctgcgc tccacgcgag gccgcaccac ctcgcgttga ttccccgctc
       61 tccttgccgg cgcaagggag aagattttgt atcgccgcaa ccaacagtag acatactatg
      121 gggagcggaa cagttgtggg gaaccgcgtc tttatcgcag agaacgcaac cgtcatcggg
      181 gatgtgacgc tcgccgacga cgtgaacatc tggttcgggg ccgttgtccg ggccgaccgg
      241 gatacgatca cggtgggatc gggctcgaac atccaggaca acgccgtcgt ccacaccacg
      301 atagggttcc cggtcgatat cgggtcggac gtctcggtcg gccatggcgc catcctgcac
      361 ggctgcatca tccacgaccg ggtgctcgtc gggatgggcg ccatcgtcct gaacggcgcg
      421 gtggtggggg aaggctccat catcggcgcc ggcagcgtgg tgaccgaggg aaaagagatc
      481 ccgccgaatt cgctggtcct cggggtcccc ggcaaggtgg tcagagagac gacgcccgag
      541 cagcgggacg gtatcacccg taacgcacgc gaatacgtca aactcgcagg gaggtaccgt
      601 cgtggctgat gtcgtcgtca tcggtgccgg ggtcgccggc atccaggcgg cgctcgacct
      661 tgcggaccac aatatccacg tccacctcgt cgagcgcgaa ccgaccatcg gcgggcatat
      721 ggcccagctc gacaagacgt tccccacgaa cgactgctcg atgtgcatcc tctctccaaa
      781 gatggtggat gtggcccggc acccgaacgt cacgatccac acctgctccg aggtcgaggg
      841 gatcgagggc gaggtcggaa ccttccgtgt ccgggtcagg aagcatcccc gctacatcat
      901 agagagcgag tgcaacggct gcggggactg catccagatc tgcccggtcg aggtctacaa
      961 ccggttcgat gccggcatcg gcgtccggaa ggcgatctac aaaccccacg cccaggccgt
     1021 ccccgacatc gtcatcaagg ataaggacca ctgcatcgag tgcggtctct gctacgatgt
     1081 ctgcgggagg gaagcgatcc tccacgagga cgaggagcgg gtcgtcgata tcgaggcggc
     1141 gagcatcgtc gttgcgaccg gttatgcgac gtttgatgcc gggaagaagg cgcagctccg
     1201 ctacctcctc atccccgacg tcatcacgag ccttgagttc gagcggatga tcaacgcgag
     1261 cggcccgacg ggcggccgct taaaaaggct ctcgaacggc aacgtccccc ggagcatagc
     1321 gttcgtccag tgtgtcggct cccgcgacat ctccgccggc cgcccctact gctcgggagt
     1381 ctgctgcatg tacgcgatga agaatgccat gctcatccgc gagaagaacc cggagatcga
     1441 ggtgaccgtc ttctacaacg acatccgggc atacggcaag ggctacgagg agtactacga
     1501 gcgggcgaag gacctcgggg tccggttcat ccgcgggttc ccgggcgagg tgctcgaggg
     1561 gaacgactgc ctgatgatga ccgcggagaa caccgagacc ggtgaagtgg agacggttca
     1621 ccccgacctc gtggtgctct ccgtcgggct tgagcctgcc cggggagcgg aagaggtcgc
     1681 ccggatgctc ggcataccgc aggacgagtc cgggttcttc gaggttgccg accagaaact
     1741 cggccccgtg ctcacggtga gacccgggat ctacgtggcg gggacggcca ccgcgccgcg
     1801 ggatataccc gactccgtcg ccatgggcga ggcggcagcg atgcgggcct acctcgacac
     1861 ggtcagggcg ggatgaatat gctggagttg gacgacctct gcaccatcgc gtgggaggag
     1921 gagaccggaa gcatcatata catcgaccag accctcctcc ccggcaggtg cgagcagatg
     1981 cggtgcacca ccgttgagga cctcgccagg gcgatccggc gactcgagat ccggggagcc
     2041 ccggcgctcg gtatcgccgg tgcgatgggg gtggccctcg cggccgcccg gagcaccgag
     2101 aggagcctgg accggttcct cgcggatgtc ggccgggccg ccgacctcct caggggcaca
     2161 cgcccgaccg cggtcaacct cgcctggggg atcgaccgcg tgataaggaa gattgcggtg
     2221 gctgcatcgg tcgacgaggc gaagaccgtt gccgccgccg aggcaaaggc cgttgccgat
     2281 gaggacgaac tggtctgccg gaggctcggt gcgttcggcg agggcctctt acccgacgac
     2341 tgcacggtcc tcacccactg caacgcgggc gctctcgcct gccggtgctg ggggacggcc
     2401 ctcggtgtga tccggtcggc ggtcgcggcc gggaagaacg tgcgggtgat cgcctgcgag
     2461 acccggccgc tgaaccaggg ctcccggctc acctgctggg agctcctccg cgatggcatc
     2521 gacgtgaccc tcatccccga ctcgtcggcc gcatacctga tgcggaaggg cagggtcgac
     2581 ctcgtcatcg tcggtgcgga caggatcacg cgggacgcgg tcttcaacaa ggttggaacc
     2641 tacatgcatg ccgtcaccgc ccgccaccac gggataccgt tctacgttgc ggcgcccctc
     2701 tccacgttcg acctctcgcg ggtggagagc gagatcgtcg tcgaggagcg ggaccgctcc
     2761 gaactcgcgt gctcggggga ccggcaactt gcgcccgacg gggtgaacgt gctcaactac
     2821 gccttcgatg ccaccccact cgacctcgtc gacgcgatcg tcaccgagat cggggtgctc
     2881 cggccgccgt atgccgaatc gttccggctt gccgggagga gggagggagt ccggccatga
     2941 gcattctcga ctccggggcc tgggtcggtc tgatgaccct catcggggag attacgttct
     3001 tcctcatcct cggcatggtg cttgcggcgg tcgctctcgc tatcatcgcc accgcctcga
     3061 tcacccgggg gaagttctac ctcccccgga tccttatacc cggcatggtc cttctcgagg
     3121 ggctcgtgaa ggcgatctgc aaacttctcg gcctggatga caaggacctc atcacgttct
     3181 tcataaccct tcggaacacc atgaacacaa aggccttcgc cgggacgccg gtggaacagc
     3241 gggcggtctt tctgccccag tgcctgcggt cggcggagtg tcccgctcac ctgacgccgg
     3301 agggcctcaa atgcaggcac tgcggccgct gcacggtcgg agagaatgta gcgtggctgg
     3361 aagggatcgg gtatcgcgtc ttcatcgtcc cgggttcgac gttcatcaaa cggatggtca
     3421 agaagtatcg cccgaaggcg atcatcggcg tcggctgcct catggaggtg aaggatggga
     3481 tcgatatgtc cgaccggata gggatcaccg ccatcggcgt cgtgaacttc aaggatggct
     3541 gcgtggagac ggttgccgac tgggccggtg tgcgggatgc cgccctgctc ggtatcgaac
     3601 ccccatcagg tgccgtagac tttcacggcc ctgccgaata accgctcgct cgtgaccttg
     3661 ccgtagacgt atatcgtctc ggcgctgttg acgtcgccga ccctgacgcc gggccgtatg
     3721 atgatgttcc cgcccgcccg gatggagtgg aggcaggcgt tatcgcatac ggtggcgtct
     3781 ttctggatcc ggagcggccc tttgatcttc gcgtcatgac cgatgacgat actctccgcc
     3841 tcgacgtagc cgcctaccgt ggagtagggt ccgagttcga ggcaaccggc aacgaccatg
     3901 ttgccccaga tatgcgtctc cgggggggcg atgaagttcc cgtctatctt tacgtttccg
     3961 tcaaaaaagg aacctctcgg cgctatgtag gtgttcccat gccggtaaac tttcatcggt
     4021 actaactctg ccccggaata cataaaatga tcagtgggat tttccggtgc atcgtggggc
     4081 gatatggata aatctcgcca gcctatgtca ccgcagcaac cgcaaagacc gcgagcgctg
     4141 caaacatacc ggcccgcagg atcgatgtcg ccctggactc cctgacgcag tccggcgtcg
     4201 tgcacctgac cccccgggca gccccggcga gtatgatgat atcgacgacc gcgatggcgg
     4261 caaggtagaa ggggccccac cagtcgccgg aggggaggag acttgccagc acggcgccgc
     4321 aggcgcaggc gaacgcgaag agccccgtcc gccggatccc gacgatcatg gggagggtgc
     4381 gtgcgccccc cgcggcatcc ccatccacgt cttccgcatc tttgaggatc tcccgggcaa
     4441 gggttgcaag aaacgcgatc gccgccagag atatattctg aatgagtccc tcgatgcccg
     4501 caaaggcacc gccgaagagg aagacgcttg ccgtcaggta ggcgacggcc acgttgccga
     4561 tgcccggtgt ccgtttcagc caggcggcgt acaggatcag gatcagggcg tttgccaggg
     4621 cgatcagaag gcagagcgcc gtcgtcaggg tggcgagggc gatgcccccg gcaaagagcg
     4681 ctaccgcgta gatctttgcg ccggaaaggc tgatcgcccc ggagggtatc ggccggtcgg
     4741 gacggttgat gcgatcgata tcgatgtcgt agacatcgtt gatcacgttc cccgcggcgg
     4801 tgatgagcgc gacgaccgcg gcaagcagga gggagggcag gatgagggtt cccgtggcga
     4861 cgaggtagcc gatgagggcg gtaaaaccgg caacgacggc gttgtgcggg cgggtaatcc
     4921 tgacgaaggc agagacgctc atgctcaacg gatcaggttc tcgggcggaa gataaataac
     4981 caaatggtct ctttcggaga aacgacgggc ttggggggat tcggaccccc gacattcagc
     5041 ttaggaggct gacgccatat cctggctagg ccacaagccc atacaccgga gtaccagtgt
     5101 ttctcacaag aaggtataag gatatcgagc atccaccttt tccagaagta tggatctcgt
     5161 tgaggttacg gagggaagaa cccggttctc tgtgccggag caggatcctc acctccagtt
     5221 cccgccgggg agcggcccgg tcttttataa cgcgcgtatg gagatgaacc gggatgccac
     5281 cgtcctcctg ctctccgtcc tgaggccgga gaattacctc gacgccatgg gggcgtccgg
     5341 ggcccggggg ctccgggtgg cctgtgaggt tggtattccg gtgacgatca acgacaggag
     5401 ccagcgggcg gtcgaccttg cccggcagaa catcgaggcc ctgggccttg tcgccgatgt
     5461 cacccggcgc gacgcaaacg cgctgatgag cgaacgaaag ttcgatgccg tcgacctcga
     5521 ccccttcgga accccggcgc cgttcgtcga ctctgcagcc cggagcgccg aaagatacct
     5581 cttcgtcacc gccaccgaca ccgcgccgct ctgcggggcg cacctgaaag ccggcatgcg
     5641 ccgatacttc tcccggccgc ggaacaccga gtaccacgcc gaagtcggcc tccggacact
     5701 tctcgggttt gtcgtgcgcg aggtgatcaa gtacgaccgc ggggtggaac cgctcttctg
     5761 ctacgcgcac gagcatttcc atcgcctgca cctcaggctc cgggaagggg cggcggcggc
     5821 cgaccggtcg cttgcacgga tcggctacgt gatgcagtgc ccccgctgcc tctaccggac
     5881 ggagcagacc ggtatgctcc ccgaaccgga ggggtgcgcg ctctgcggtg cggacctcgt
     5941 gccggtcggc cccctctgga cggggaggat caacgacgat gagatgcttg ctgcgatgca
     6001 ggaggcgctt ccccgtgtca cggtcgggac cgggccgcgg atcgctcggc tgctcgctac
     6061 ctgccggcag gaactggata cgtcgagcca ctacgactat cacgtcatag caaaatcgct
     6121 ccgggtctcg cccggcggta tcgggacggt cgtcgaccgc ctcgtggccc tcgggtaccg
     6181 ggcgagccgc gcgcactact cgggaacggc cataaagacc gatgctccgc ttcccgtcct
     6241 ggagagtgtg atcagcggcg ggtgacgatg gagaggacgt ctccatccgc gagctggtgg
     6301 gtgatcccga cgcgctgcgc gtcgtgcttt gccgaccggc cccagacttt tgcgtaccgg
     6361 aacttgtccg cgaactcccg gtggaggcgg ttgcagacat cctcgaccgt cgccgggctc
     6421 cggatgatca gcggttcgtc catatccgcc gggccgccga ccggcttcag gtagacccgc
     6481 atgaatccca ggtcatcgaa tatggcgtct tttaagtcct cgatgcggta gccgctgtgt
     6541 gccgatacca tgatgggggg ttcgccgaac cgttcggtca gttcttcctc gatctccgcc
     6601 cgcgtcttcc cgtccaccag gtcgaccttg ttgaccgcga tgaacgcggg gacgtagacc
     6661 cggttgccga tcatcgcatc gatgaagtcg tcctgcgtgg caccgtcgcg gaccaggacg
     6721 tcggcgttga cgatcttgtt ctccgcgagg atcgagcgga cctcttcgag atcgagatcg
     6781 acggcgccga cggtgttcaa ccggacaccg ccgttccccg tctttcttat ggtgatatcg
     6841 ggtctctgcc ggttgatccg gatgccggca tcgtagagct cgcggagaag gacgtccgtg
     6901 tgctgctcgt tgaagacgtc gaccaggacg aggatcaggt cggcgctccg caccacggcg
     6961 atgacctctt tcccgcgccc tttgcccatg gcagcgccgg cgatgaggcc gggaatatcc
     7021 aggacctgga tctttgcgcc ccggtgctcc atcgcgccgg ggatgacgga gacggtcgtg
     7081 aaggcgtaat ccgccgtctc gctgagatcg aaaccggtga gcctgttgag cagcgtactc
     7141 tttccgacgg acgggaaacc gaccaggaca acggtggcgt cccccgactt cttcacggag
     7201 taaccctcgc cgcccccgga ggaggccatg gcccgggcta ccgcctcatc gcgaatcttc
     7261 gcgagtttcg ccttgaggcg accgatgtgc ttggaggtcg ccttgttgta cggggtattc
     7321 ttgatctcgt cttcaagttc ccgtatctgc tcttcgacgc tgctcattac tgccggtata
     7381 ggtacgccgg gcagctactt attgatgtct gtgatggaga acgtggattg atggaggaat
     7441 cccggcccgg atagggctcc gattacaggg ttgccggcag gggtgaaagg aggggggtac
     7501 gcgacatttt taaatagtag cgtatcagat aatgtagagc acattggtgc aatgcgctgc
     7561 tatagtgtag accggccaat catgagggac tctcactccc tcgactgggg ttcgaatccc
     7621 catagcagca t
//
LOCUS       DAOSII010000091         7569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_8942_length_7569_cov_43.254059, whole genome shotgun sequence.
ACCESSION   DAOSII010000091 DAOSII010000000
VERSION     DAOSII010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7569
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8942_length_7569_cov_43.254059"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PLV90_07490"
     CDS             <1..467
                     /locus_tag="PLV90_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOF96995.1"
                     /translation="EVLMRAVKGITELITMPGLVNLDFADVRTVMERGGVAMIGMGES
                     DSEDKAVDSVKKALRSPLLDVDISGATAALVNVVGGPDMTMYEAEGVIQEVYDRIDPD
                     ARIIWGAQVDPDMQGRMRTLLVITGVRSPQIYGRNEKSMPRMMKQFEIDFLK"
     gene            474..683
                     /locus_tag="PLV90_07495"
     CDS             474..683
                     /locus_tag="PLV90_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase SEC61 complex subunit gamma"
                     /protein_id="HOF96996.1"
                     /translation="MMDYKEKFEEVTSFKIEEELFKKYWRVLKLARTPTRDEFSKIAI
                     VAAAGIMLIGLVGFIIYEILLVLPR"
     gene            689..1162
                     /locus_tag="PLV90_07500"
     CDS             689..1162
                     /locus_tag="PLV90_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor Spt5"
                     /protein_id="HOF96997.1"
                     /translation="MAESVNRVYAIKTTAKQERTVADNIEKVTREQKDIHVTAVMVPD
                     ELKGYVLVESPDSIARIEQLVELIPHARAVVQGSTALSEVAHFLAPKPVVSGITEGTI
                     VEIIAGPFKGEKAVVKRIDSGKEEITVELYESMVPIPITVRGDSVRVVERIEDSR"
     gene            1339..1815
                     /locus_tag="PLV90_07505"
     CDS             1339..1815
                     /locus_tag="PLV90_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOF96998.1"
                     /translation="MAETVEVLVPGGKATAGPPLGPALGPLGINVKAVVDEINKKTAD
                     FNGMQVPVTVTVDDKRNFTIEVGIPPTTALVIKEAGIAKGSAEPGTQVAGDLPLEAAV
                     RIARMKFDGMLSYDLKSAVKEVLGTCVSVGVTVEGQKPRDVIRAINDGTYDSVLVA"
     gene            1867..2508
                     /locus_tag="PLV90_07510"
     CDS             1867..2508
                     /locus_tag="PLV90_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOF96999.1"
                     /translation="MVDKTSIQKAVKSALDRAPERKFKESVDITVNLRNIDMSQPKNR
                     IDETILLPNGLDNVKIAVLGKGDITTQAKDVGVDLIIGPEEIERLGGAPREARKVAEE
                     YRFFLAETAMMPLVGRYLGVRLGPRGRMPMPVPQGMDIRPVVQRLRSSVKIRTKDKKV
                     FHAKVGSAGMDPAKIAENIDAVLRRVESVLEGGALNIHSVYVKTTMGPAVRVI"
     gene            2508..3533
                     /locus_tag="PLV90_07515"
     CDS             2508..3533
                     /locus_tag="PLV90_07515"
                     /inference="COORDINATES: protein motif:HMM:NF003098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOF97000.1"
                     /translation="MALYTHHLPRWKKEEVEAIKRGVAEHTLVGVVDMYGIPASQVQQ
                     IRRNLRGTATVKMARNTLIEHAFTELGGDVEGLKEHAEGQSALIFTNENPFKLFKLLE
                     KTKTKMAAKPGETAPEDLVVPKGPTSFKPGPIVGELQQVGIPAAIEGGKVKIRETKTV
                     VKKGEVISKKVADALVKLGIKPMDVGLVLQAAYYRNTIFTPDLLAIDQEAYANNIALA
                     ARQAFNLSVNAAIPTPTTIGAIIGRAVREARSVGVEAPIYEKDIADLIIGRAQREMLV
                     VALAAQDHGFELDEATLKAASAATAAAPAAAPAAVEAKPEEEETEEEKKEEEEESGMA
                     GLGALFG"
     gene            3558..3875
                     /gene="rpl12p"
                     /locus_tag="PLV90_07520"
     CDS             3558..3875
                     /gene="rpl12p"
                     /locus_tag="PLV90_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein P1"
                     /protein_id="HOF97001.1"
                     /translation="MEYIYAALLLHNAGKDVTEENVTAVLKSAGIAVQDARVKALVAA
                     LEDVNIEEAISKAAVAPVAAAPVAAAAPAAAAPAAEEEAEEESKKEEEEESGMAGLGA
                     LFG"
     gene            4255..4623
                     /locus_tag="PLV90_07525"
     CDS             4255..4623
                     /locus_tag="PLV90_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOF97002.1"
                     /translation="MSKKVMIIDDNEKFLRITGLLIQSFGYTLITLNDPREGLEMISR
                     DPPSLILLDIMMSPMDGSQFLDERRKIPGALDIPVVICSAWRLTEEELAPYRNEIVGV
                     IAKPVEPALLREILKDHLGV"
     gene            4693..>7569
                     /locus_tag="PLV90_07530"
     CDS             4693..>7569
                     /locus_tag="PLV90_07530"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOF97003.1"
                     /translation="MPVNFTAPDIKRLVSKRDLGGLITAIGSEDKDTRWMAAGGLGEL
                     CDPRAIEPLIRALADVDPDVRWKAAEALGSIGDTRATEVLIPLLRDPDGIMRLQAVWA
                     LGKIRDQSATAHIIPFLTDPDYDVRIAAVWALGAIGGGRAVAALREMLLDRHPGIRSK
                     AAESLEKCGWKPVDDRERGAFAFARRDWDEVSHYGRRLEDILIWALDDRYFDVRMHAA
                     RILGTTRSRSAIPHLLKALDDPEECVTYEAAAALAEIGNAESKKALVHGLESPHLSTR
                     KVAAGALDRLAWQPRSIYHESLFLSAKDDWIGLVRLKKHGVDPLARELQERCGVERAS
                     IAKALGAIGSPAIDAMIGLLGSPDPDIRWRAASILGDSRDRRAVEPLIAALADPDEHV
                     SSSVAIALGEIRDERAVDPLIRAYATGGPGLRRDIVAALGRTRSERAVAAIIAASGDE
                     NPVVRSSAVRAMGRIRDVRVLPALIPFFQDPDPAIRFEAVVAIRPYSGREIDLLLTRA
                     LEDPDAGVRREAARQAGSRGVTTAVQPLVGLFGDPDTAVRKAAARALERIGWEPSDSR
                     EMLAVLIAKEDWEEIQRQGLLYSPAGDPKSGGAGLLPGGLQGREGVVSPGPADQEKQI
                     PQEDREVSPRRGVNVATHEAVPDLQYFIDILNDPRVDLPARLKAVEALGNLGDLRGVR
                     PLMQALRDPESEIRGNAALSLGMLGDHKAFGSLVVALEDPVFEVKRRVAEALSVLKPG
                     GAVLPLAELARSPNPGSRMLAVTVLGDFEEDDGVRALLGALNDEYSGVKSAALSSLLK
                     LVDYWGSRIPVFLKDGDPAIRKNVLTALNTIIGEDKAISLLIPLLHAGNFSVRREAVL
                     ALEGMGWRPGLPEEHAMVLIAGREWDEVVILGKQAESALIGALFDTDREIRDGAVASL
                     GMIGDDETVRSIRAAMRGGVDLQARGIHAAMKAASLIEEKEAGRG"
BASE COUNT         1552 a         2089 c         2510 g         1418 t
ORIGIN      
        1 acgaggtgct gatgcgggcg gtcaagggca tcaccgagct gatcacgatg cccggcctcg
       61 tgaacctcga ctttgccgat gtccggaccg tcatggagcg gggcggcgtc gcgatgatcg
      121 gtatgggcga gagcgacagc gaggacaaag ccgtcgattc ggtcaagaag gcgctgcgct
      181 ctccgctgct cgacgtggac atatccgggg cgaccgctgc tctcgtcaac gttgtcggcg
      241 ggccggacat gaccatgtac gaggctgaag gcgtcatcca ggaggtctac gaccgcatcg
      301 acccggatgc ccgcatcata tggggtgctc aggtcgatcc cgatatgcag ggccggatgc
      361 ggacgctcct cgtcatcacg ggtgtccggt caccacaaat atatgggaga aacgagaaga
      421 gtatgccacg catgatgaag cagtttgaga ttgacttcct gaagtgagcc ggtatgatgg
      481 actataaaga gaagtttgag gaagtaacgt cgttcaagat cgaagaggag ctcttcaaga
      541 agtactggcg ggtgctgaaa ctggcacgga cgccgacccg cgacgagttc tcgaagatcg
      601 cgatcgtggc ggcggccggc atcatgctca tcggccttgt cggttttatc atatacgaga
      661 tcctgctggt actgcccaga tgatggatat ggctgagagc gtcaacagag tgtatgcgat
      721 aaagacgacc gcaaagcagg agaggacggt agccgacaac atcgagaagg tgacccggga
      781 gcaaaaagat atccacgtga cggccgtcat ggtccctgac gagctcaaag gctacgtcct
      841 tgtggagagc cctgattcca tcgcccggat agagcagctg gtggaactga tcccgcacgc
      901 gcgcgcggtg gtccagggtt cgaccgctct ctcggaggtc gcgcatttcc tggcacccaa
      961 accggtggtc agcggcatca ccgagggcac catcgtcgag atcatcgcgg gtcccttcaa
     1021 aggcgagaag gcggtcgtga agaggatcga ttccgggaag gaagagatca cggtcgagct
     1081 ctacgagagc atggtcccca tcccgatcac ggtccgcggc gactccgtcc gcgtcgtgga
     1141 acggatagaa gactcccgct gaaagggtat ctcccccctc ctttttcggt tcgtttgcgc
     1201 gtccccatcc tatcggcgac gcggttcccc cggtcaacgg caatggaagc cgcccggcac
     1261 tatattttta aatcccctta cgctgacctt agataaccca ggcaactatt cccctgagga
     1321 ataagctggt gaatttgtat ggcagaaacg gtcgaggtat tggtgcccgg cggcaaggct
     1381 acggcaggtc cgcctcttgg acctgcactc ggacctctcg gtatcaacgt gaaggctgtc
     1441 gtcgacgaga tcaacaaaaa gacggccgac ttcaacggca tgcaggtgcc ggtgacggtc
     1501 acggtcgacg ataagaggaa cttcacgatt gaggtgggca ttccgcccac gacggccctc
     1561 gtcataaagg aggccggtat cgcgaagggt tccgcagagc cgggcactca ggtggcagga
     1621 gatctgccgc tggaggccgc tgtccgtatc gcccgtatga agtttgatgg catgctctct
     1681 tacgacctga aatccgcggt caaagaggtt ctcgggacgt gcgtgagcgt cggcgtcacc
     1741 gtcgagggcc agaagccccg cgacgtgatc cgggccatca acgacggaac atacgacagt
     1801 gtactcgtcg catagatgtc agacaaccat agaagatcat gaggtcgtgt actatggagg
     1861 caaaggatgg ttgataaaac cagtatacag aaagccgtga agtcggcact ggatagagct
     1921 ccggagcgga agttcaagga gagcgtggat atcaccgtca acctcaggaa tatcgatatg
     1981 tcccagccca agaatcgtat cgacgagacg attctcctcc cgaacggtct tgacaacgtc
     2041 aagattgccg ttctcgggaa gggcgacatc accacccagg caaaggacgt gggtgtggac
     2101 cttatcatcg gacctgagga gatcgagcgg ctcgggggcg caccccggga ggcgcgcaaa
     2161 gtggcggagg agtaccggtt cttccttgcc gagacggcca tgatgcctct cgtcggccgc
     2221 tatctcggtg tcaggctcgg cccgcgcgga cggatgccga tgccggtccc gcagggcatg
     2281 gatatccggc ccgtcgtcca gcgtctccgg agctccgtga agatccggac gaaggacaag
     2341 aaagtcttcc acgcaaaggt cggttctgcc gggatggacc cggcaaagat cgccgagaac
     2401 atcgatgcag tcctccggag ggtcgagtcg gtcctcgaag gcggggcgct gaacatccac
     2461 tcggtctacg tgaagacgac catggggccg gctgtgaggg tgatctgatg gcgctttaca
     2521 cgcatcacct gccccggtgg aagaaggaag aggtcgaggc gatcaagcgc ggtgttgcag
     2581 agcatacgct cgtcggcgtc gtggatatgt acggcattcc tgcctcgcag gtccagcaga
     2641 tccggcggaa cctccgcggc acggcgacgg tgaagatggc caggaacacg ctcatcgagc
     2701 acgcgttcac cgaactcggc ggcgacgtcg aggggctgaa ggagcatgcc gaaggccaga
     2761 gcgccctgat cttcacgaac gagaacccgt tcaagctctt caagctgctt gagaagacaa
     2821 agaccaagat ggcggcaaag cccggcgaga ccgctcctga ggatctcgtc gttccgaagg
     2881 gcccgaccag cttcaagccc ggccccatcg tgggcgagct ccagcaggtg ggcattcctg
     2941 cagccatcga gggcggcaag gtcaagatcc gggagacgaa gacggtcgtg aagaagggcg
     3001 aggtcatcag caagaaggtt gccgatgccc tcgtgaagct cggtatcaag ccgatggacg
     3061 tcggtctcgt cctgcaggct gcctactacc gtaacaccat cttcacgccg gaccttctcg
     3121 ccatcgacca ggaggcctac gcgaacaaca tcgcacttgc tgcccggcag gcgttcaacc
     3181 tctcggtgaa tgcggctatt ccgaccccga cgacgatcgg cgcgatcatc ggcagggcgg
     3241 tgcgcgaagc ccggagcgtg ggtgtcgagg cgcctatcta cgagaaggat atcgccgacc
     3301 tgatcatcgg gcgggcacag cgcgagatgc tggtcgttgc gcttgcggcg caggaccacg
     3361 gtttcgagct cgatgaggcg accctgaagg cggcctcggc tgcgactgcc gcggcacccg
     3421 ctgcagcccc ggcagccgtc gaggcaaagc ccgaggaaga ggagacggaa gaggagaaga
     3481 aggaggagga ggaagagagc ggtatggccg gtctcggcgc cctcttcggc taatattatt
     3541 taaaaggtga atgaactatg gagtatatct acgctgcact gctcctgcac aacgcaggca
     3601 aggacgtaac tgaagagaat gtgaccgctg tcctgaagtc ggccggcatc gccgtccagg
     3661 atgcccgcgt gaaggcgctc gtcgccgcac tcgaggacgt gaacatcgag gaggccatca
     3721 gcaaggccgc cgtcgctccg gtcgccgccg cacctgtggc agccgccgcc cctgccgccg
     3781 ccgcgcccgc ggccgaggaa gaggcggaag aagagtccaa gaaggaagag gaagaagaga
     3841 gcggcatggc cggtctcggc gccctcttcg ggtaatcttt ttttttcggt ttgtttgcga
     3901 tttttgcggc gtgattttcc cgggagccac ggccggacgg gttttgggaa tacatatgat
     3961 ggggccgaga gcatatccgg caatccgtga tggttcgtgt tgggtccggg ccggtgggca
     4021 ccggcgtatc atcgacaaag ttgccgtatc cgtcggaccc ctgttctgca gattccggca
     4081 ggtgaacgcc tggttaatca taatttcatc gattttcccc ttccggtcgt gtatgttcgt
     4141 ccgatgtata tagataagta taaattatcc tttggatgat tgttcattca agaccggatc
     4201 ccctgcaggg ccgcagtcgc tcgccccggc aggaatcagg gaggtaaatc gggtatgtcg
     4261 aagaaagtga tgataatcga cgacaacgaa aaattcctcc gaatcaccgg gctgcttata
     4321 caatcatttg gatacaccct catcacgctc aatgacccgc gagaggggct tgagatgatc
     4381 tcaagagacc ccccctcgct gatactcctc gatatcatga tgtcgccaat ggatggttcg
     4441 cagttcctgg acgagcggag gaagatcccc ggagcactgg atataccggt ggtgatctgt
     4501 tcggcgtgga ggctgaccga ggaggaactt gccccttata ggaatgagat agtcggggtc
     4561 atcgccaaac ccgttgaacc cgctctattg agggagatcc ttaaagacca tctcggggtg
     4621 taatggcgtg aagctcggaa aggtttgtta caggcgccta ccggaaggat ctttttcccg
     4681 gggtggagta taatgccggt taacttcacc gcccccgaca tcaagaggct cgtctcaaaa
     4741 cgggatctcg gcggtcttat aacggctatc gggagcgaag ataaggatac gcggtggatg
     4801 gcagcggggg gacttgggga gttatgcgac ccgcgtgcga ttgaacctct tatccgggcg
     4861 ctggccgatg tcgatccgga tgtcaggtgg aaggccgcgg aagcgctcgg ttctatcggg
     4921 gatacccggg cgacagaagt tctcatcccg ctcctcaggg accctgatgg gattatgcga
     4981 ttgcaggcgg tctgggcgct cggaaagatc cgggatcaga gtgcgacggc gcatatcatc
     5041 cccttcctga ccgatcccga ctacgacgtc agaatcgcag cggtctgggc gctcggggcc
     5101 atagggggcg ggcgtgccgt tgccgcactg cgggagatgc tgctggatcg gcacccgggc
     5161 atccggtcga aggcagctga atccctggag aagtgcgggt ggaaaccggt tgacgaccgt
     5221 gagcggggtg cttttgcatt tgcacgaagg gattgggatg aagtgtctca ctatggtcgg
     5281 aggctggagg atatcctgat ctgggcgctg gacgaccgtt attttgatgt ccggatgcat
     5341 gccgcaagga tccttggtac aacgagaagc cggagcgcta tcccgcacct ccttaaggcc
     5401 cttgacgacc cggaagagtg cgtcacttat gaggcggcag ccgcacttgc ggagatcggg
     5461 aacgccgaat caaagaaggc tcttgttcat gggcttgaga gcccgcattt aagtacccgg
     5521 aaggtcgcgg caggggcact cgatcgcctg gcatggcagc cacggagcat ctaccatgag
     5581 agcctgttcc tgagcgcaaa ggatgactgg atcggtcttg tgcggctcaa gaaacacggc
     5641 gtcgatcccc tcgcccggga actgcaggag cgatgcggtg tggagagggc gagcatcgca
     5701 aaggcgcttg gggctatcgg cagcccggcg atcgatgcca tgatcgggct gcttggaagt
     5761 ccggatcccg atattaggtg gcgggcagcc tcgatactgg gagatagccg cgatcggcga
     5821 gcggtcgagc cgctcattgc ggccctggca gatccggatg agcacgtctc ttcttccgtc
     5881 gcgattgccc tcggtgagat ccgtgacgag agggcggtgg atcccctcat cagggcgtat
     5941 gcaaccggag gcccgggtct ccgccgcgat attgtcgcgg cgcttgggag aacaagatcg
     6001 gaacgggccg ttgcggctat catcgcagca tccggcgatg aaaatcccgt tgtccgcagt
     6061 agcgccgtga gggccatggg ccggatcagg gatgtgcggg ttctccccgc cctgatcccg
     6121 ttcttccagg accccgatcc tgccatccgg tttgaggcgg tagtggcgat tcgcccgtat
     6181 tccggccggg agatcgatct cctcctcacc cgggccctcg aagacccgga tgccggggta
     6241 cgacgggagg cggcccggca ggccggcagt cgtggggtga caaccgcggt tcaaccgctt
     6301 gtcgggctct ttggagaccc ggacaccgcg gtgaggaagg cggccgcaag ggcgcttgag
     6361 aggattggct gggagccttc ggattcccgg gagatgttag ccgtcctgat tgcaaaagag
     6421 gactgggagg agatccagcg ccagggcctc ctctactctc ctgccgggga cccgaagagc
     6481 ggcggcgcgg ggttgctgcc cggtggcctg cagggcaggg agggagtggt ctctcccggc
     6541 cccgccgatc aggagaagca aatcccgcag gaggaccgtg aggtctctcc acggcggggg
     6601 gtgaatgtcg caactcacga agctgttcca gatctgcagt atttcatcga catcctcaac
     6661 gaccctcgcg tggatctgcc ggcccggctc aaggctgtcg aggcgctcgg gaatctcggg
     6721 gatctcaggg gagttcgacc gttgatgcag gctctacgcg atccggaatc ggagatccgc
     6781 ggaaacgcgg ccctctctct tggtatgctg ggcgaccata aggcctttgg ttccctggtc
     6841 gttgcactcg aggatccggt gtttgaggtg aagcggcggg ttgccgaggc gctctcggtt
     6901 ctgaaacccg gaggtgcggt cctcccgctt gccgagctgg cgaggagccc gaaccccggc
     6961 agccgcatgc ttgccgtcac ggtcctcggc gactttgaag aggatgatgg ggtgcgcgcc
     7021 ctgcttggtg cgctcaacga tgagtattcc ggggtgaagt cggctgcgct gtcgtcgttg
     7081 ctcaaactgg tcgactactg ggggtcacgt ataccggttt tcctgaaaga cggggatccg
     7141 gccatccgga aaaacgttct gactgctctg aataccataa taggggagga taaggccatt
     7201 tctctgctta tcccgctcct ccatgccggc aacttctccg tccggcgaga ggcggttctc
     7261 gcgctcgagg ggatgggctg gaggccgggc ctgccggaag agcatgcaat ggtgctgatc
     7321 gccggcaggg agtgggatga ggttgttata cttggaaagc aggcggagag tgccctgatc
     7381 ggcgctctct tcgatacgga ccgggagatc cgggatgggg ctgtggcgtc tctcgggatg
     7441 atcggggatg atgagacggt ccggtcgatc agggcggcga tgaggggggg tgtggatctc
     7501 caggccaggg ggatccatgc cgcgatgaag gcggcgagcc tcatcgagga gaaagaggcg
     7561 gggagaggc
//
LOCUS       DAOSII010000092         7528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_8991_length_7528_cov_52.352469, whole genome shotgun sequence.
ACCESSION   DAOSII010000092 DAOSII010000000
VERSION     DAOSII010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7528
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8991_length_7528_cov_52.352469"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..>1290
                     /locus_tag="PLV90_07535"
     CDS             4..>1290
                     /locus_tag="PLV90_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOF97004.1"
                     /translation="MKKIVIKGAREHNLQNITVELPRDRLIVLTGVSGSGKSTLAFDT
                     IYAEGQRRYVESLSAYARQFLGLMRKPDVDSIDGLSPAISIEQKTTSKNPRSTVGTVT
                     EIYDYLRLLFARVGTPFCPEHHIPIEAQSPEKIADHIAATMAGTVTILAPVVRQKKGT
                     YQQVFKDLDREGYTRVRVNGEIRRTDEEIALERYQKHDIEVVIDRLAADDRSRLVEAV
                     ENALAKSEGLVIAVDEDGREEICSSLMACPVCGIAFEELQPRMFSFNSPFGACEECNG
                     LGIRMEFDPDLIIPDRSKCIADGAVALYRNFLDGYRSQYLGAVAKHFGFSVLTPIRDL
                     TDRQYNALMFGSPEHLRLSMQMRNGDAWSRSGAWEGLAPQAERLYHQTGSDYRRQELE
                     KFMRVLPCPKCGGKRLKEKVLAVKVNGRSIVDVADLS"
     assembly_gap    1291..1390
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1391..2639
                     /locus_tag="PLV90_07540"
     CDS             <1391..2639
                     /locus_tag="PLV90_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF97005.1"
                     /translation="KLLETLQHLRDLGNTLVVVEHDEETIRSADWVVDMGPGAGLHGG
                     KVVAEGPPDAIAGDPASLTGRYLSGDLRIDIPAARRGSDRFIRLLGCRGNNLKNIDVN
                     IPIGVLTVVTGVSGSGKSTLIYETLYRALMRELHGSREPPGEHDSLTFDDEIDKVIVI
                     DQSPIGRTPRSNPATYTKVFDEIRKVFAETKEAKVRGYKPGRFSFNVKGGRCEACQGD
                     GLIKIEMNFLPDVYVECEECKGSRYNRETLEVKYRGKSIADVLDMSVEEAIALFENVP
                     SIRNRLETLSRVGLGYIKLGQSSTTLSGGEAQRIKLTRELAKRATGKTIYLLDEPTTG
                     LHFHDVKNLIAVLDDLVARGNTMVVIEHNLDVIKSADHIIDLGPEGGDAGGEIIATGS
                     PEAVAKVTASHTGAFLKEILRRP"
     gene            2636..4186
                     /gene="uvrC"
                     /locus_tag="PLV90_07545"
     CDS             2636..4186
                     /gene="uvrC"
                     /locus_tag="PLV90_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOF97006.1"
                     /translation="MIDLQSLPAEPGCYLFSDAEGTIIYVGKAKNLRRRVSSYFSRHD
                     IDPKTRRLVATIAGLDFIVTDTEVEAFILENTLIKKHQPKYNIDLKDSKSFAYIHITD
                     EVYPRIHVARGPDGSGRYYGPFVSARERDDLLRLLKSIFGLRSCRRLPRRACLRAHIG
                     SCSAPCTGAIGEEDYRERVKRAEAVLKGDIAGLIRTLREEMAVLAGRQEYERAMELRD
                     QIAALEGLRERQHVDRQKTYNEDIVNYIVSEGTVFLMLFNVDRGTLAGKREYTFDETE
                     GFLEEFLARYYAGNEPPEEVILPEAVDDAITGYLSHLRGGRVRAVVPQRGEKKNLLDL
                     VRKNVEIGFFGDRIKLEELKRRLHLPDTPVAIECFDISHLSGTAMVGSMVRFLRGKPD
                     KRNYRRFRIRTVEGVDDFAAIAEVVRRRYSRLQKEGGELPDLILVDGGKGQLAAAVAV
                     LQELGIKVPIAAIAEREEEIFVPGFSHPLPLARNEKASLFLQEIRDEAHRFAITYHRS
                     LRRKAATP"
     gene            4183..6123
                     /gene="uvrB"
                     /locus_tag="PLV90_07550"
     CDS             4183..6123
                     /gene="uvrB"
                     /locus_tag="PLV90_07550"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOF97007.1"
                     /translation="MTAFQLKADFKPTGSQPDAIRKLTGGLARGERYQTLLGVTGSGK
                     TFTIANVIAEVQKPTLVIAHNKTLAAQLYNEFKSFFPDNRVEYFVSYYDYYQPESYIP
                     KRDLYIEKDAQINPKIEQMRLAATASVLSRPDTIIVASVSCIYGLGNPANFQGMGFEV
                     KVRDRVRRDDIIRRLVEIQFERNDIELAPGRFRVKGDTIDIVPGYFNNIIRIELFGDE
                     VDRISEIDRNSGQRLETMDYFFVYPARHFVAPEEEKERAIVSIEEELEEWLPGLGMLE
                     SHRLRQRTLYDIDMIRETGTCKGIENYSRHFDGRKAGEQPYCLLDYFPEDFLMVIDES
                     HQTLPQVRGMYHGDYSRKKSLVDYGFRLPSAFDNRPLKFDEFSGYMRNVIFVSATPGD
                     YELGRSSVAEQIIRPTGLVDPAVEVRPIEGQIPDVIAEIRATIDRGDRILLTTLTKRL
                     AEELSEYLAEQGIKTRYLHSEINTIERTEIIRQLRLGKYDVLVGINLLREGLDIPEVG
                     FVGILDADKEGFLRDARSLVQTIGRAARNVNAKVVLYADTMTDSIRKAITETERRRTM
                     QLAYNARHGITPQTIRKPIREREVDIADIKHVPKSEIPNLIIELEADMREAAERLEFE
                     RAIALRDTIKKLQEGGAGEIRR"
     gene            complement(6139..6360)
                     /locus_tag="PLV90_07555"
     CDS             complement(6139..6360)
                     /locus_tag="PLV90_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOF97008.1"
                     /translation="MKRLYRSRTDRWIAGICGGIGEYLEIDPNVIRVIWAIGTAVTGF
                     VPGIVVYILLWIILPESGMARPVEVPVGA"
     gene            complement(6446..>7528)
                     /locus_tag="PLV90_07560"
     CDS             complement(6446..>7528)
                     /locus_tag="PLV90_07560"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HOF97009.1"
                     /translation="TLVISPLIALMKDQVDDLQARGIGAEALNSSGSYRTTCRILSEL
                     EENLIQILYVSPEKAVSDDFLALVASLPVTLIAIDEAHCISMWGHQFRPEYRSLAVLK
                     ERLSGVPTVALTATATPDVRDDIARQLDLADPAVYVGSFNRENLRYAVIAKGEDPYAE
                     LRTCLQDRKAGAGIVYTATRDGAETLSARLQADGMAALPYHAGMTAAARARTQDRFLH
                     GKAGVVCATSAFGMGIDKPDVRFVVHYDMPDTLEAYYQESGRAGRDGATGDCILFYHD
                     DDAKRLRSFIDRDLPSEFRRDVARSKLQSMVEYCTATGCRRERLLAYFGEDFNAPCGG
                     CDACAPQTGKVRQGRRKRGAVRSASG"
BASE COUNT         1480 a         2427 c         2337 g         1184 t
ORIGIN      
        1 agaatgaaga agatcgtcat caagggggcg cgggagcaca acctccagaa catcaccgtc
       61 gaactcccgc gcgatcggct catcgtcctc accggcgtct ccgggtcggg aaaatccacg
      121 ctggccttcg acaccatcta cgccgaaggc cagcggcgct acgtggagtc gctctccgcc
      181 tacgcgcggc agttcctcgg cctgatgagg aaaccggacg tcgacagcat cgacgggctc
      241 tcgcccgcca tatccatcga acagaagacg acatccaaaa acccccggag caccgtcggc
      301 acggtcaccg agatctacga ctacctccgg ctcctctttg cccgggtggg aacaccgttc
      361 tgcccggagc accacatccc catcgaggcc cagtcgcccg agaagatcgc ggaccacata
      421 gccgccacca tggccgggac ggtcaccatc cttgccccgg tcgtgcggca gaagaaagga
      481 acctaccagc aggtcttcaa ggacctcgac cgggaaggct acacccgggt ccgggtcaac
      541 ggtgagatcc gccggaccga cgaggagatc gccctcgaac gctaccagaa gcacgacatc
      601 gaggtggtca tcgaccgcct tgccgccgac gaccgctcaa ggctcgtcga ggccgtcgaa
      661 aacgcccttg caaagtcgga agggctcgtc atcgccgtcg acgaggatgg gcgcgaggag
      721 atctgttcgt cgctcatggc ctgcccggtc tgcggtatcg cgtttgagga actccagccc
      781 cgtatgttct ccttcaacag ccccttcggg gcctgcgagg agtgcaacgg actcgggata
      841 aggatggagt tcgatcccga cctgatcata ccggacagga gcaaatgcat cgccgacggc
      901 gccgtcgcgc tctaccggaa cttcctcgac ggctaccgga gccagtacct cggcgccgtc
      961 gcgaaacact tcgggttctc ggtcctgaca ccgatccggg atctcaccga ccgtcagtac
     1021 aacgccctga tgttcggttc gcccgagcac ctccgcctct ccatgcagat gaggaacggg
     1081 gacgcgtggt cgcgctccgg ggcatgggag gggcttgctc cgcaggccga gcggctctac
     1141 caccagaccg ggtcggatta ccgccgccag gaactcgaga agttcatgcg ggtcctcccc
     1201 tgcccgaagt gcgggggaaa gaggctcaag gagaaggttc tcgccgtcaa agtgaacggg
     1261 agatccatcg tcgacgtcgc cgacctctcc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn aaagctcctc gagaccctgc agcacctccg cgacctcggg aataccctgg
     1441 tcgtcgtgga gcacgacgag gagacgatcc ggagcgccga ctgggtcgtg gatatggggc
     1501 ccggggccgg gctccacggc ggaaaggtcg tcgcagaggg gccgcccgac gccatcgccg
     1561 gagatccggc ctctctcacc gggcgctacc tctcgggcga cctccggatc gacatacccg
     1621 ccgcccggag agggagcgac cggttcatcc gccttctagg ctgccggggg aacaacttaa
     1681 aaaacatcga cgtcaacatc cccatcggcg tcctgacggt cgtcaccggg gtctcggggt
     1741 cggggaagtc cacgctcata tacgagacgc tctaccgggc gctgatgcgg gaactccacg
     1801 gttcgcggga acccccgggg gagcacgaca gcctcacgtt cgacgacgag atcgacaagg
     1861 tgatcgtcat cgaccagagc cccatagggc ggacgccgcg ctcgaacccg gcgacctaca
     1921 ccaaggtctt tgacgagatc cgcaaagtct ttgccgagac gaaggaggcg aaggtccggg
     1981 gctacaagcc cggccggttc tcgttcaacg tcaaaggcgg acggtgcgag gcctgccagg
     2041 gcgacgggct catcaagatc gagatgaact tcctccccga cgtctacgtc gagtgcgagg
     2101 agtgcaaggg gtcgcgctac aaccgcgaga ctctcgaggt gaagtaccgg gggaagtcca
     2161 tcgccgacgt cctcgatatg agcgtcgagg aggcgatcgc cctcttcgag aacgtcccct
     2221 cgatccggaa caggctcgag acgctctcac gggtggggct cggttacatc aagctcggcc
     2281 agagctccac caccctctcg gggggcgagg cgcaacggat caaactgacc cgcgaactcg
     2341 caaagcgggc gacaggaaag acgatctacc tcctcgacga gccgacgacc ggtctgcact
     2401 tccacgacgt gaagaacctc atcgccgtcc tcgacgacct cgtcgcccgg gggaacacga
     2461 tggtcgtgat cgagcataac ctggacgtga tcaagtcggc cgaccatatc atcgacctcg
     2521 gtcccgaagg cggggacgcc ggcggcgaga tcatcgcgac ggggagccct gaggcggtgg
     2581 caaaggtgac ggcgagccat accggggcgt tcttaaagga gatcctcagg agaccatgat
     2641 cgacctccag agcctgccgg ccgagcccgg gtgctacctc ttctccgacg ccgaagggac
     2701 catcatctac gtcgggaagg caaagaacct ccggcggcgc gtatcgagtt acttctcccg
     2761 gcacgacatc gacccgaaga ccaggagact cgtcgccacg atcgccgggc tcgacttcat
     2821 cgtcaccgat accgaggtcg aggcgttcat cctcgagaac accctgatca agaagcacca
     2881 gccgaagtac aacatcgatc tcaaggactc gaagagcttc gcctacatcc acatcaccga
     2941 cgaggtctac ccccgtatcc acgtcgcccg gggacccgac gggagcggcc gctactacgg
     3001 gccgttcgtc tccgcacggg agcgcgacga cctcctccgg ctcttaaagt cgatcttcgg
     3061 cctccggtcc tgcaggcgcc ttccccggcg ggcgtgcctc cgggctcaca tcggttcgtg
     3121 cagcgccccc tgcacgggag ccatcggcga agaagactac cgggagcggg tgaagagggc
     3181 cgaggcggtc ctgaagggag atatcgccgg actcatccgg acgctccggg aagagatggc
     3241 ggtcctcgca gggcggcagg agtacgagcg ggcgatggag ctccgcgacc agatcgcagc
     3301 cctcgaggga ttgcgcgagc gccagcacgt ggaccggcag aagacctaca acgaagatat
     3361 cgtcaactac atcgtgagcg aggggaccgt attcctcatg ctcttcaacg tcgaccgggg
     3421 aacgcttgcc gggaagcgcg agtacacgtt cgacgagacg gaggggttcc tcgaggagtt
     3481 ccttgcccgg tactacgcgg ggaacgagcc cccggaagag gtgatcctcc ctgaagcggt
     3541 ggacgacgcc atcaccggct acctctccca ccttcgcggc ggcagggtcc gggcggtcgt
     3601 gccgcagcgg ggcgagaaga agaacctgct cgacctggtg cgaaagaacg tcgagatcgg
     3661 gttcttcggc gaccggatca aactggagga actgaaacgc cgcctgcacc tcccggatac
     3721 ccccgtcgcg atcgagtgct tcgatatatc ccacctctcc gggacggcga tggtggggtc
     3781 gatggtccgg ttcctccggg gcaagcccga caaacggaac taccggcgct ttaggatacg
     3841 gacggtcgag ggcgtagacg acttcgccgc catagccgag gtggtccgcc gccgctactc
     3901 gcgcctgcag aaggagggcg gcgaactccc cgacctgatc ctcgtcgacg gcgggaaggg
     3961 gcagctcgcg gcggccgtcg ctgtgctcca ggaactcggg atcaaggttc cgatcgcggc
     4021 catcgcggag cgcgaagaag agatcttcgt gccggggttc tcccaccccc tgccgcttgc
     4081 gcgaaacgag aaggcgtcgc tcttcctcca ggagatccgc gacgaggcgc accggtttgc
     4141 gatcacctac cacaggagcc tgcggaggaa ggcggcaaca ccatgacggc atttcaactg
     4201 aaggcggact tcaagccgac cggctcgcag cccgacgcga tccggaaact caccggcggc
     4261 cttgcccggg gcgagcgcta ccagaccctg ctcggggtca cggggtcggg gaagacgttc
     4321 acgatagcaa acgtcatcgc cgaggtccag aaacccacgc ttgtcatcgc gcacaacaag
     4381 acccttgcgg cccagctcta caacgagttc aagtcgttct tccccgataa ccgggtggag
     4441 tacttcgtct cctactacga ctactaccag ccggagtcct acataccgaa acgcgacctc
     4501 tacatcgaga aggatgccca gatcaacccg aagatcgagc agatgcgcct tgcagccacc
     4561 gcatcggtcc tctcgcgccc cgacaccatc atcgtcgcct cggtctcctg catatacggt
     4621 cttggaaacc ccgcgaactt ccaggggatg gggtttgagg tgaaggtccg cgaccgggtg
     4681 cggcgggacg acatcatccg gagactcgtc gagatccagt tcgagcgaaa cgatatcgaa
     4741 cttgcgcccg gccggttccg ggtgaagggg gataccatcg atatcgtgcc cggctacttc
     4801 aacaacatca tccgtatcga gctcttcggg gacgaggtgg acaggatcag cgagatcgac
     4861 aggaactccg gccagcggct cgagacgatg gactacttct tcgtctaccc ggcccgccac
     4921 ttcgtcgccc cggaggagga gaaggagcgt gcgatcgtat cgatagagga ggaactcgag
     4981 gaatggctcc cgggcctcgg catgcttgag tcgcaccgcc tgcgccagcg gaccctctac
     5041 gacatcgaca tgatccgcga gaccgggacc tgcaagggga tcgagaacta ctcccgccac
     5101 ttcgacggga ggaaggccgg cgaacagccc tactgcctgc tggactactt ccccgaggat
     5161 ttcctgatgg tgatcgacga gagccaccag accctgcccc aggtccgcgg catgtaccac
     5221 ggcgactact cgcggaagaa gtcgctcgtg gattacgggt ttcggctccc gtcggcgttc
     5281 gacaaccggc cgctcaagtt cgacgagttc tcggggtata tgcgaaacgt catattcgtc
     5341 tcggcaaccc cgggcgacta cgaactcggg cgttcgagcg tcgcggagca gatcatccgc
     5401 ccgaccgggc tcgtggaccc ggcggtcgag gtccggccga ttgaagggca gatcccggac
     5461 gtgatcgccg agatccgggc cacgatcgat cgcggcgacc gcatactgct gacgaccctt
     5521 accaaacggc tcgccgagga actctccgag tacctggccg agcaggggat caagacccgc
     5581 tacctccact ccgagatcaa caccatcgag cgaaccgaga tcatccggca gctccgtctc
     5641 gggaagtacg atgtcctcgt ggggatcaac ctcctccgcg agggtctcga tatacccgag
     5701 gtcgggtttg tcgggatcct cgatgccgat aaagaagggt tcctccgcga cgcaagaagc
     5761 ctggtccaga ccatcgggcg ggccgcccgg aacgtgaacg caaaggtcgt gctctacgcg
     5821 gacaccatga ccgactcgat caggaaggcc ataaccgaga ccgagcggcg gcgcacgatg
     5881 cagcttgcct acaacgcccg gcacggcata acgccgcaga cgatccgaaa accaatccgg
     5941 gagagagagg tcgatatcgc cgatatcaag cacgtcccga agtcggagat accgaacctg
     6001 atcatcgagc tcgaggccga tatgcgggag gcggccgaac gcctggagtt cgagcgggct
     6061 attgcgcttc gtgatacgat caagaagctg caggaggggg gtgcgggaga gatccggcgg
     6121 tgaggccggc cctccccttc acgctccgac gggcacctcg accgggcgcg ccatcccgct
     6181 ctccggcaga atgatccaga gcaggatgta gacgacgatc ccgggtacaa aacccgtgac
     6241 cgcggtgccg atagcccaga tcacccggat gacgttcgga tcgatctcaa ggtattcccc
     6301 gatgccgccg catatgccgg cgatccaccg gtcggttctc gagcggtata acctcttcat
     6361 ggcaactccg cagggtcatt ttaccgtatg cgtaataggt gtttcccccg ggagttcggg
     6421 agaataggtg ccggaggccg gcacctcatc cggacgcgga acgaaccgca ccgcgcttcc
     6481 tgcgcccctg ccggaccttc ccggtctgag gggcacaggc gtcgcaaccg ccgcagggag
     6541 cattgaagtc ctccccgaag taggcgagga gcctctccct ccggcacccg gtcgcggtgc
     6601 agtactccac catgctctgc agtttcgagc gtgcgacgtc gcgccggaac tcggacggga
     6661 ggtcgcggtc gatgaaggac cggaggcgtt ttgcgtcgtc gtcgtgatag aagaggatgc
     6721 agtcgcccgt cgctccgtcc ctccctgccc ggccgctctc ctggtagtag gcctcgagcg
     6781 tatccggcat atcgtagtgg acgacgaacc tgacgtccgg cttatcgatg cccatgccga
     6841 acgcactcgt agcgcagacg accccggcct ttccgtgcaa aaaccggtcc tgtgtcctgg
     6901 cccgggcggc cgccgtcatg ccggcgtggt agggcaacgc tgccatgccg tcggcctgga
     6961 gacgggccga gagcgtctcg gccccatccc tcgtcgcggt atagacgata ccggcgccgg
     7021 cctttcggtc ctgcaggcag gtgcggagct ctgcatacgg gtcttctccc ttggcgatga
     7081 cggcatacct gaggttctcc cggttgaagc tcccgacgta gaccgccggg tctgcgagat
     7141 cgagttgccg cgcgatatcg tcgcggacgt ccggcgtcgc ggtcgccgtc agggcgaccg
     7201 tcggcacgcc ggagagacgc tccttgagga ccgcaagcga ccggtactcc ggccggaact
     7261 ggtgccccca catcgatatg cagtgcgcct cgtcgatcgc tatcagcgtg acggggaggg
     7321 atgcgacgag agcgaggaag tcgtcgctga ccgccttctc cggcgagacg tagaggatct
     7381 ggatcaggtt ctcttcgagt tcggagagta tccggcatgt cgttctgtag gacccggaag
     7441 agttcagcgc ctcggcgccg atcccgcgtg cctgcaggtc gtcgacctgg tccttcatca
     7501 gggcgatgag aggcgatatc acgagcgt
//
LOCUS       DAOSII010000093         7452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_9100_length_7452_cov_57.756388, whole genome shotgun sequence.
ACCESSION   DAOSII010000093 DAOSII010000000
VERSION     DAOSII010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7452
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9100_length_7452_cov_57.756388"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /gene="cofH"
                     /locus_tag="PLV90_07565"
                     /gene_synonym="cofG"
     CDS             <1..360
                     /gene="cofH"
                     /locus_tag="PLV90_07565"
                     /gene_synonym="cofG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842904.1"
                     /note="7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase
                     subunit 2; catalyzes radical-mediated transfer of
                     hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to
                     5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form
                     7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO); functions
                     in F420 biosynthesis along with cofG; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase subunit CofH"
                     /protein_id="HOF97010.1"
                     /translation="FTEFVPLSFIHENTALYRAGLARAGATGREDLLMFAVARLFLDN
                     FDHVQASWVKLGTKMAEAALLAGADDLGGTLFEESISREAGARGTSYLDPGDMRRMAE
                     DLGRMLRQRTTTYDLLD"
     gene            476..631
                     /locus_tag="PLV90_07570"
     CDS             476..631
                     /locus_tag="PLV90_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HOF97011.1"
                     /translation="MADLISLAILFFILAIIFALLGVRGIAGMSMSIAKWLVIIFIIL
                     AILSLLF"
     gene            complement(712..1263)
                     /locus_tag="PLV90_07575"
     CDS             complement(712..1263)
                     /locus_tag="PLV90_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOF97012.1"
                     /translation="MSVLGISGSMRKNGNTALLVTTILDRVREAGIETEFLTLAGMDI
                     RPCTGCEACKSAKWCATEDDDWAGVAEKMIGCEVLVLGAPTYYYDINGQTKNLIDRTY
                     SLYHDRRLAGRKAVSVAVCADRGCERTLETIEGFLNTHEFSYLGYVCGKGYAPGDVRK
                     DERAMERARQVADTIIRYLRPED"
     gene            complement(1260..1715)
                     /locus_tag="PLV90_07580"
     CDS             complement(1260..1715)
                     /locus_tag="PLV90_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOF97013.1"
                     /translation="MDRGVYALILKNPHREIRIGALGRREFAEGSQIYVGSAHGSGGL
                     ARVERHIRLALRRDRSPRWHIDYLLLDPQFALVAAITAATDRDCECALARAIGGSSAP
                     GFGCSDCACPSHLFFRPTDPVEEVLTAFESLSLDARITRVKSEGNEHKV"
     gene            complement(1708..2310)
                     /locus_tag="PLV90_07585"
     CDS             complement(1708..2310)
                     /locus_tag="PLV90_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOF97014.1"
                     /translation="MPVRWIASGTTYANSFVYGNVLVDAGVLPMAVEPYAGAIDTIVL
                     THAHYDHTAHVKEIARLCGDAAVCIHEADAPGLVNDTRSLAMIFGARSPGIAPDVLLS
                     DGDPVGSLRVIHTPGHTPGGICLYDPAAKVLFSGDTVFAGGSFGRYDFPGGDRRALIA
                     SIERLAALDVEGLYPGHGEPAPTGGGRHIAAAREAFRFYG"
     gene            2336..3628
                     /gene="thiC"
                     /locus_tag="PLV90_07590"
     CDS             2336..3628
                     /gene="thiC"
                     /locus_tag="PLV90_07590"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOF97015.1"
                     /translation="MVLMHTLISACLRGVPPEVEAIAREEDLSPHHAARAVARGRIVV
                     PANPVRPHRLCAIGEGCRVRVNVNVGTSGIRCDEDLEVEKAKAALNEGADALMDLSTG
                     GDLVRIRRRILALDAPVGTVPVYEAVRRAGSAADLDADLLFSVVREHCRQGVDFLTLH
                     CGVNRDALASLEADPRTMGVVSRGGAFHVAMMAATGEENPLYAEYDYLLEILAEHDVV
                     VSLGDGMRPGAFVDAGRLAKTTEYLTLGHLAKRAIAAGVQRMIEGPGHIPADQIGYNV
                     RMIKELTDGAPLYLLGPLVTDVAPGYDHVVGAIGGVIACMHGADFLCMVSPAEHLALP
                     DIRDIVEGTRAARIAAHVGSLSRAAGSTRNHEARMAEARRALDWEKQFEAALFSGEAR
                     RIHERDGALETCSMCGDLCAVKMVREILKAPPEERMEP"
     gene            complement(3762..4154)
                     /gene="purE"
                     /locus_tag="PLV90_07595"
     CDS             complement(3762..4154)
                     /gene="purE"
                     /locus_tag="PLV90_07595"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOF97016.1"
                     /translation="MVDVAVICGSASDTPVAEKVFAVLQEHGISYDYQVISAHRDPER
                     LDGYVKASDARVFIAIAGLSAALPGVVASKTERPVIGVPVSGKLMGFDALLSIVQMPK
                     GVPVACVGVDNGENAGLLAVRILGAGRT"
     gene            4288..5418
                     /locus_tag="PLV90_07600"
     CDS             4288..5418
                     /locus_tag="PLV90_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOF97017.1"
                     /translation="MEHEPLLMIPGPVPIPQRVRAAMTRQAINHRGPEFGAAYADTVR
                     TLKTLFGTAHDLYIISGSGSAGMEAAVANFARDRRIVSLVNGKFGERFAKIGERYGMV
                     ALLESEWGTPLDLAGLERELEAGAEIVTMVHNETSAGIKNPAPEVAKLARKHDALFIM
                     DGVTSIGGDDVRMDEWGVDIAVVGSQKCLAAPAGLAAIAVGDRAWDRICEKRPFYLDM
                     AAYRKSGSGTPMETPYTPAVPLFLALQEACTIIEEEGIPARIARHRRMADAVRAAAKG
                     WGVDLFPTLDARHAYSNTATAMRIPEGVTDKDLRGTVKKFGIEIAGGQDRLKGRIFRI
                     GTMGGIGAQEILATLTAVEFALKKSGFDAGAGVEAAAEVLLG"
     gene            5415..5876
                     /gene="ribC"
                     /locus_tag="PLV90_07605"
     CDS             5415..5876
                     /gene="ribC"
                     /locus_tag="PLV90_07605"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOF97018.1"
                     /translation="MKIGIADTTFARVDMGRIAADEIRKHASVGLERSVVPGIKDLPV
                     ACKKLIEERGCEIVMALGMPGGAEKDKICAHEASQGLILCQLMTNKHIIEVFVHEDEA
                     KDAKELAWLMERRTREHAANAVRLALYPKELEKLAGTGQRQGFEDVGPARP"
     gene            5935..6342
                     /gene="ribH"
                     /locus_tag="PLV90_07610"
     CDS             5935..6342
                     /gene="ribH"
                     /locus_tag="PLV90_07610"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOF97019.1"
                     /translation="MTVRLGFVVAEFNRDITYMMEIEAREHANFLGAEVAGAIYVPGA
                     YDMPLAIKKLLGQEDIDAVVTIGCVIEGATQHDEIVVQHAARKIIDLSLEFGKPVSLG
                     ISGPGMTRMEATERIDYARRAVESAVKMVQRLG"
     gene            6339..>7452
                     /locus_tag="PLV90_07615"
     CDS             6339..>7452
                     /locus_tag="PLV90_07615"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOF97020.1"
                     /translation="MRGLSEKIAGIPPSATIEISNAAKRMAQEGIDVISLSIGEPDFD
                     TPAHIKDACIDALRRGETHYAPSAGIPELTGAIAEKITRENGFSAKPDEVIVTCGAKD
                     AIYEAMEAVLNPGDEVLILDPSWVSYEPCARIAGGLARHHALSPETFQVDDTLLEAVG
                     PRIRMIVVNSPSNPSGAVLNADSLRLIADICRDNDIFALSDEIYEKLVYGKTPVSIAS
                     LPGMAERTITINGFSKAYAMTGWRLGYAVAPRPVIRQMEKVQQHTISHPTTFAMFGAV
                     AALEGPQDCVESMRREFERRRDYIIPALCDLGYTTAPADGAFYAYVNVGGDDMAIARS
                     WLHDAHVAVTPGTAFCTPGWLRLSYATSMENLMEAVG"
BASE COUNT         1314 a         2417 c         2456 g         1265 t
ORIGIN      
        1 ttcaccgagt tcgtccctct ctcgttcatc cacgagaata ctgctctcta ccgggcgggt
       61 cttgcacgag ctggggcgac ggggagggag gatctcctta tgtttgcggt cgcccgtctc
      121 ttcctcgaca acttcgacca cgtccaggcg tcctgggtga agctcgggac gaagatggcg
      181 gaggcggcgc ttcttgccgg cgccgacgac ctcgggggga cgctctttga ggagagcata
      241 tcccgggaag cgggcgcgcg gggcaccagc tacctggacc cgggcgatat gcggcggatg
      301 gccgaagatc tcggccggat gctccggcag cggacgacaa cctacgacct cctggactga
      361 tatcgcctga ggatcggatc ttctcccgat ccaccgctat tctcggaaag atatatatgc
      421 ccacggatgg agtgtggctc ctcaacgccg actggcgatt gggagggaga cagatatggc
      481 agatcttatc agccttgcaa tcctcttctt tatactggcg attatctttg ccctgctcgg
      541 ggtaaggggc atcgctggaa tgtcgatgtc gatcgcgaag tggctggtca tcatcttcat
      601 catcctcgcg atactatcgc tgctcttctg accgcgggaa gggcgttcgg gctgctgggc
      661 ccggagatcc ttcccgcggt cagggatgcc ccggccgggc gtatccgctc ttcagtcctc
      721 cggccggaga tacctgatga tcgtatccgc aacctgccgc gccctctcca tcgcgcgctc
      781 atccttccgc acatcccccg gcgcatagcc ctttccgcag acgtaaccga ggtaggagaa
      841 ctcgtgggtg ttgaggaacc cctctatggt ctcgagcgtc cgttcacacc cccgatcggc
      901 gcagacggcg acggagaccg cttttctgcc cgcaagacgc ctgtcatggt agagggagta
      961 ggtccggtcg atcaggttct ttgtctgccc gttgatgtcg tagtaatagg tcggggcgcc
     1021 gaggaccagg acttcgcagc cgatcatctt ctctgccacg ccggcccagt cgtcgtcctc
     1081 cgtcgcgcac cacttcgcgc ttttgcaggc ctcgcagccg gtgcagggac ggatatccat
     1141 accggcgagc gtcaggaact cggtctcgat gcctgcctcc cgcacccggt cgaggatggt
     1201 ggtgacaagg agtgcggtgt tgccgttctt ccgcatgctt ccggatatgc caagaacact
     1261 cataccttat gctcgttccc ctcgctcttg actcttgtga tccgggcatc cagggagagg
     1321 ctctcgaacg cggtgaggac ctcctcgacc ggatcggtgg ggcgaaagaa gaggtgcgag
     1381 gggcaggcgc agtcgctgca cccaaatccc ggagcagaag acccgccgat ggcacgggcg
     1441 agggcgcatt cgcagtcccg gtcggtcgcg gcggtgatgg cggcgacgag agcgaactgc
     1501 gggtcgagga ggaggtagtc gatgtgccac cggggggagc ggtcacggcg aagggcgagc
     1561 cggatgtgcc gctccacccg ggcaaggccg ccgcttccgt gggcggagcc cacgtagatc
     1621 tgcgagcctt ccgcgaactc tcttctcccg agagcgccta tgcggatctc ccggtgcggg
     1681 ttcttgagga tgagggcgta gacccctcta tccatagaac cggaacgcct cccgggcggc
     1741 cgcgatatgc ctcccgccgc ccgtgggggc gggctcgccg tgcccggggt agagcccctc
     1801 gacatcgagt gctgcgagcc gttcaatcga cgctataagt gccctccggt cgccgccggg
     1861 gaagtcgtag cgcccgaacg atccgccggc gaagacggtg tcccccgaga agagcacttt
     1921 tgccgccgga tcgtagaggc agatcccgcc cggcgtgtgc cccggcgtgt ggatgacgcg
     1981 gaggctcccg accgggtcgc cgtcggagag gaggacgtcg ggggcgatcc cgggcgatcg
     2041 ggcgccaaag atcatggcga ggctccgggt gtcgttgaca aggccgggag cgtccgcctc
     2101 gtggatgcag acggcggcgt cgccgcagag ccgggcgatc tccttcacgt gggcggtgtg
     2161 gtcgtagtgg gcgtgggtga ggacgatcgt atcgatagcg ccggcatacg gctccaccgc
     2221 catcgggaga acgcctgcat ccacgaggac gtttccgtaa acgaacgagt tggcgtaagt
     2281 cgtgccgctc gcgatccacc ggactggcat gtagactaat atggcctccg gacaaatggt
     2341 tcttatgcat accctgatct ccgcatgcct gcgcggggtc cccccggagg tggaggcgat
     2401 cgcccgcgag gaggatcttt ccccgcacca tgccgcgcgg gccgtcgccc gcgggcggat
     2461 cgtcgtcccg gcaaaccctg tgcggccgca ccggctctgc gccatcggcg agggctgcag
     2521 ggttcgggtc aacgtgaacg tcgggacatc gggtatccgg tgcgacgagg acctcgaggt
     2581 cgagaaggcg aaggcggccc tcaacgaggg tgcggatgct ctgatggacc tctcgaccgg
     2641 cggcgatctc gttcgcatcc ggcgccggat cctcgccctc gacgcgcccg tcgggacggt
     2701 cccggtgtat gaagcggtcc ggcgggcggg gagcgcagcg gatctcgatg ccgacctgct
     2761 tttctcggtg gtccgggagc actgccggca gggcgtggac ttcctgacgc ttcactgcgg
     2821 cgtgaaccgc gacgcgctcg cctccctcga ggcggatccc cggacgatgg gcgtcgtctc
     2881 ccgcggcggg gccttccacg tggctatgat ggccgcgacc ggtgaagaga accccctcta
     2941 cgccgagtac gactacctgc ttgagatcct tgccgagcac gacgtggtcg tgagcctcgg
     3001 ggacgggatg cgccccggcg cgttcgtgga tgccggccgc ctcgcaaaga cgaccgagta
     3061 cctgacgctc gggcatctcg cgaagagggc gattgccgcc ggggtgcaac ggatgatcga
     3121 ggggccgggg catataccgg ccgaccagat cggctacaac gtccggatga tcaaggagct
     3181 gaccgacggc gcaccgctct acctgctcgg cccgctcgtg accgacgtgg cgccgggcta
     3241 cgaccacgtc gtgggggcga tcggcggcgt gatcgcctgc atgcacggcg ccgacttcct
     3301 ctgcatggtc tcgccggccg aacatctggc gctgccggat atccgcgaca tcgtggaagg
     3361 gacgcgggcg gcaaggatcg ccgcacacgt cgggagcctc tcccgcgccg ccgggagcac
     3421 caggaaccat gaagcccgta tggcggaggc gcgccgggcg ctcgactggg agaaacagtt
     3481 cgaggcggcg cttttctccg gggaggcccg gcgcattcac gagcgggacg gggcgcttga
     3541 gacctgctcg atgtgcggcg acctctgcgc cgtaaagatg gtccgggaga tcctcaaggc
     3601 gccgccagaa gagcggatgg aaccgtaaga ggcgttcgct gccggcacta cggggcgccc
     3661 ttccctccga actgcccgtc gatccaggcg gttatcgcgc tccggctccg ccggtaggcc
     3721 gcgaggacct tgtcaggtcc gggaagtcgg catggagcgt atcaggtgcg gcccgcgccg
     3781 aggatccgca ccgcgagcag gccggcattt tcaccgttgt ccacgccgac gcaggccacc
     3841 gggacgcctt tcggcatctg gacgatcgag aggagggcat caaaccccat cagtttcccg
     3901 ctcacgggga caccgatcac cgggcgttcc gtcttcgagg cgaccacgcc cgggagggcc
     3961 gcggaaagcc cggcgatagc gatgaagacc cgggcgtcgc ttgccttcac gtacccgtcc
     4021 agcctctcgg gatcccggtg cgccgagatc acctggtagt cgtaggatat gccgtgctcc
     4081 tgtaagactg caaacacctt ctccgctacg ggtgtatccg aagcggagcc gcagatcacc
     4141 gcgacatcaa ccatacctcc ctctggtatg ctcctgcccc ttcatctctt taccgatatc
     4201 gcaccgtggt gaggttgata agagtttaca tccaagttcc ttcaggaact gcgtccgtcc
     4261 ccgacgagac gatacggact gatcaccatg gaacacgaac cactcctcat gattcccggc
     4321 cccgtgccca tcccgcaacg ggtacgcgcc gccatgacgc ggcaggccat caaccatcgc
     4381 ggccccgagt tcggcgccgc atacgcggat accgtccgga ccttaaagac cctttttggc
     4441 accgcacacg acctctacat catcagcggc tccgggagcg ccggtatgga ggccgccgtc
     4501 gcaaactttg cacgggacag acggatcgtc tcgctcgtca acggtaagtt cggcgaacgt
     4561 tttgcaaaga taggtgagcg ttacggtatg gtcgccctcc tcgagtccga gtgggggacg
     4621 cccctcgatc ttgcaggtct tgagcgggaa ctcgaggccg gggccgagat cgtcacgatg
     4681 gtccacaacg agacgagcgc cggcatcaag aaccccgcgc ccgaggtcgc gaaactcgcc
     4741 aggaagcacg acgccctctt catcatggac ggtgtcacct ccatcggcgg cgacgacgtc
     4801 cgcatggacg agtggggcgt cgatatcgcc gtcgtcgggt cgcagaagtg cctcgcagcc
     4861 cccgcgggcc ttgccgccat cgccgtcggc gatcgcgcct gggaccggat atgcgaaaag
     4921 cgtcccttct acctcgatat ggcggcctac cggaagagcg gaagcggcac cccgatggag
     4981 accccctaca ccccggcggt cccgctcttc cttgccctcc aggaggcctg cacgatcatc
     5041 gaggaagaag gcatcccggc ccggattgcc cgccaccgcc ggatggccga cgccgtccgc
     5101 gccgcggcga agggatgggg cgtcgacctc ttcccgacgc tcgatgcacg ccacgcctac
     5161 tcaaacaccg cgaccgcgat gcggatcccc gaaggcgtca ccgacaaaga tctccgggga
     5221 acggtcaaga agttcggtat cgagatcgcc ggcggtcagg accgcctcaa gggcaggatc
     5281 ttccggatcg gcaccatggg cggcatcggg gcgcaggaga tccttgcaac cctcacggcc
     5341 gtcgagtttg ccctcaaaaa gtccggattc gatgccggcg ccggcgtcga ggcggccgcc
     5401 gaggtgctgc tcggatgaag atcgggatcg ccgacaccac gtttgcccgg gtggatatgg
     5461 gcaggatcgc cgccgacgag atccgaaagc acgcaagcgt gggccttgag cgttccgtcg
     5521 tcccgggcat caaggatctc ccggtggcgt gcaagaagct gatcgaggag cggggctgcg
     5581 agatcgtgat ggccctcggc atgcccggcg gggccgagaa ggataagatc tgcgcccacg
     5641 aggcctcgca gggcctcatc ctctgccagc tcatgacgaa caaacacatc atcgaggtct
     5701 tcgtccacga ggacgaggcg aaggatgcaa aagagcttgc ctggctgatg gagcggcgga
     5761 cgagggagca cgcggcgaac gccgtccggc tcgccctcta tccgaaggag cttgagaagc
     5821 tcgccgggac cggtcagcgg caggggttcg aggacgtcgg gcccgcacgc ccctgacgta
     5881 aagagcgatg gttatcagta tccggtgcga ttactaccac agggattgat tgatatgacg
     5941 gtaagacttg gatttgtcgt cgcggagttc aaccgcgaca tcacctacat gatggagatc
     6001 gaggcccggg aacacgcgaa cttcctcggc gcggaggtgg cgggagcgat ctacgtcccc
     6061 ggcgcctacg atatgccgct tgcgatcaag aagctgctcg ggcaggaaga catcgatgcg
     6121 gtcgtcacga tcgggtgcgt catcgagggc gccacccagc acgacgagat cgtcgtccag
     6181 cacgcggcgc gtaagatcat cgacctctcg cttgagttcg ggaaacccgt ctctctcggc
     6241 atatccgggc cggggatgac ccggatggaa gctaccgaac gtatcgacta cgccaggcgt
     6301 gccgtcgagt cggccgtgaa gatggtccag cgattgggat gagaggcctc tcagagaaga
     6361 ttgcgggcat ccctccctcc gcgaccatcg agatatcgaa cgcggcaaag cggatggcgc
     6421 aggaaggtat cgatgtcatc agcctctcga tcggggagcc ggacttcgat accccggcac
     6481 acatcaaaga tgcctgtatc gacgctctcc gccgcggaga gacgcattac gccccgagtg
     6541 ccggcatacc ggagctgacg ggcgcgattg ccgagaagat cacccgcgag aacgggtttt
     6601 ctgcaaagcc ggatgaggtg atcgtcacct gcggggcaaa agacgccata tacgaggcga
     6661 tggaggccgt cctgaacccc ggcgacgagg tgctcatcct cgatccctcc tgggtctcct
     6721 acgagccctg tgcccgtata gcgggcggcc ttgcccggca ccacgctctc tctcccgaga
     6781 cgttccaggt cgacgatacc ctgctcgagg ctgtcggccc ccggatacgg atgatcgtgg
     6841 tcaactcccc ctcgaacccc tccggcgcgg tgctgaacgc ggactctctc cggctgatcg
     6901 ccgatatctg ccgggacaac gatatattcg cactctccga cgagatctac gagaaactgg
     6961 tctacgggaa gacgccggta tcgatcgcct ccctcccggg tatggcggag cggacgatca
     7021 ccatcaacgg gttctcgaag gcctacgcga tgacggggtg gcggctcggc tacgcggtcg
     7081 cgccccggcc ggtcatccgg cagatggaga aggtgcagca gcacaccata tcgcacccga
     7141 cgacgtttgc catgttcggc gcggtcgccg cgcttgaggg gccccaggac tgcgttgaat
     7201 cgatgcgccg cgagttcgag cgccggcgcg actacatcat accggcgctc tgcgacctcg
     7261 gctacaccac cgctccggcg gacggcgcct tctacgccta cgtgaacgtc ggcggcgacg
     7321 acatggcgat cgcccggtcg tggctccacg acgcccacgt ggcggtcacc ccggggacgg
     7381 cgttctgcac ccccggctgg ctccggctct cctacgcgac gtcgatggag aacctgatgg
     7441 aagccgtcgg gc
//
LOCUS       DAOSII010000094         7350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_9268_length_7350_cov_48.349280, whole genome shotgun sequence.
ACCESSION   DAOSII010000094 DAOSII010000000
VERSION     DAOSII010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9268_length_7350_cov_48.349280"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PLV90_07620"
     CDS             complement(<1..357)
                     /locus_tag="PLV90_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HOF97021.1"
                     /translation="MGAFTAPTQTDREFHPVDKTYATTAVRKAVATGLLTRDDADLIT
                     EFVTELQASQGITTVRVNKITSHLINWRRYICPYREATIGDIFSAIATIKEARNQRGR
                     PFKQNTLHDYIMFLKRF"
     gene            complement(421..530)
                     /locus_tag="PLV90_07625"
     tRNA            complement(join(421..456,493..530))
                     /locus_tag="PLV90_07625"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(494..496),aa:Met,seq:cat)
     gene            complement(603..2078)
                     /locus_tag="PLV90_07630"
     CDS             complement(603..2078)
                     /locus_tag="PLV90_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOF97022.1"
                     /translation="MKYFDKILIANRGEIAIRVMRACRELGIDTVAIYSEPDKNALHV
                     KYADEAFFVGEAHPSKSYLNKERICDIARKSGAEAIHPGYGFLAENYRFAKLVEDEGR
                     TFIGPSWKTIEALGSKIGSKRMMREAGVPVLPGTPEGVTCLDEAKEVAAAIGYPVVVK
                     ASAGGGGIGMHIVESEEGLEEAIEGGMRIAGSAFGDPTVFVEKYLAKPRHIEVQVLAD
                     AKGHTVHLYDRECSIQRRHQKLLEEAPCPIMTPDLRERMTASAIAAAKAANYRNAGTV
                     EFLYSNGNYYFMEVNTRLQVEHTITEFITGIDIVKQQIAVAAGEDLAMGQEDISIRGH
                     AIECRINAEDPLNNFTADPGKIVRYRSPGGPGIRVDSGIHVGYAIPPHYDSMIAKLCA
                     WGSNREEAIQRMRRAIYEYVILGVKTTLPLHYAIMRNPHFVAGDTHTHFLQEEHISAS
                     LRRYLREEETRMQTLAASLRQGKKVAAITAAVDVYLRKNME"
     gene            complement(2092..3849)
                     /locus_tag="PLV90_07635"
     CDS             complement(2092..3849)
                     /locus_tag="PLV90_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/oxaloacetate carboxyltransferase"
                     /protein_id="HOF97023.1"
                     /translation="MCAAKFDTLHITDTTLRDAHQSLIATRLRTEDMLPLARAIDEVG
                     FFSVEAWGGATFDSCIRFLNDDPWERLRMLKAELKRTPIQMLLRGQNLVGYRHYPDDV
                     VEKFVEASARNGVDIFRVFDALNDIRNMKKAMEEVQNVGAHLQGAISYTTSPVHSVAS
                     FIDMAGELYSLGCNSICIKDMAGLIMPHDARDLISGIKETMDVMVNLHSHCTSGVAPL
                     SYQAAIDAGVDILDTAMSPFALGTSQPPTESVVASVIGTPRDTGIDLLPLRNVRNVCR
                     DMREKYEPLFSAIADRVDSDVLIYQLPGGMISNLISQLKEQNALNRLEEVLQEIPRVR
                     KDLGYPPLVTPTSQIVGTQAVFNVLMDGERYRNVTTEVKDYVRGLYGRPPAPISPEIR
                     ELIIDGEEPVTVRPADLLAPICGQMCKEATEQGLVTCDEDVLTYILYPSVAPSFLRGE
                     RQAEVIPKPVTAPAARIAEIPSSMEVEVDGEIFSVRIVTVEGSSVTVPGSATAPAGKG
                     RAPRGDVAGGVKSNMQGMVLQVLTQKGNAVKKGDTLIVLEAMKMENPIRSPRDGRVEE
                     IFVNAGDVVQNGDVLLIVE"
     gene            complement(3903..4556)
                     /locus_tag="PLV90_07640"
     CDS             complement(3903..4556)
                     /locus_tag="PLV90_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97024.1"
                     /translation="MQDPRLRLLSTVVLSLAAFASTMGAVVALVWWLVFTPRTKALPR
                     PGVLFSLTAMIALTALVSAWGGGPGLSYFIRMVVVLLLAAWAYTETEEGEVLAVAVWA
                     LGKRIGFEIGLIAEMGLTGLLVIRQEIDQARVALALKGIPIGARSIVPIAVLLIVTQI
                     RRADEVARLLVVRGYALGGRICPRFRTDPRDILASVSAVILGLLSCFPVRDVFILLG"
     gene            complement(4538..5230)
                     /locus_tag="PLV90_07645"
     CDS             complement(4538..5230)
                     /locus_tag="PLV90_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF97025.1"
                     /translation="MELEDLLLSRGPFTLRGSGTFEEGVHLVSGAVGSGKSTLALLLA
                     GLLHPGSGAIRRQRVSSTLLLLQFPEYHVTSPTVAEEAGSWGLVPDDVLARADLADRG
                     DDDPFHLSRGELKRLILACTFMRDPDLLMLDEPFSSLDCAAKQKACRMIEERGRGITI
                     IFSHERAVLPRVDAIWEMEGGILTALGNVPGAIPRWRHAPPYLAYALDRGARPENIRL
                     EDAREAICRIQG"
     gene            complement(5233..5904)
                     /locus_tag="PLV90_07650"
     CDS             complement(5233..5904)
                     /locus_tag="PLV90_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HOF97026.1"
                     /translation="MIRIVNLRHRIVDIPDLTVDARHVAVIGPNGSGKTTLLSICSGI
                     EEPRRGTVRLLGRAPAAVKIGWVGEFPDRTLLFSRAYDEIASSPRFRHRPCLETDERV
                     RAAAGTIGIPHLLDAKVSALSGGEKALVALAAAVVDDPEVLILDEADSHLDGGTAARI
                     QAAVKKSTAAHVLWCTQSMDTAAEADYVVFMAGGAVRHHGTPEEVFTSLEETCFYPTM
                     WRIRR"
     gene            complement(5901..6410)
                     /locus_tag="PLV90_07655"
     CDS             complement(5901..6410)
                     /locus_tag="PLV90_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOF97027.1"
                     /translation="MVYMKHRAQILAYSGTFVALIAAGSWISIPLPPVPITLQTLFVL
                     LAGIVMKRYAVIPVALYVLLGAMSLPVFHNGTAGVGVLLGPTGGFLIGFIPAALIAGL
                     AYEQESPKWRIGGLIAACAIYLSAGAVWLSYSASIPLLQAILIGVAPFIVGDTLKAAA
                     AYTIGKRVA"
     gene            complement(6482..>7350)
                     /locus_tag="PLV90_07660"
     CDS             complement(6482..>7350)
                     /locus_tag="PLV90_07660"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOF97028.1"
                     /translation="VRELRRLGYGIASSRAEGYRLESATGKLLPYEIHKKLRTRSIGR
                     QIRHFDRTASTSWIAKQLAAETDPEKLHGMVIIAEEQTGGVGRLGRAWVSPAGGIWIT
                     IVLKPKIPLDHLFMITMAGAIAIARAIRKEYNISALIKWPNDIFAGDKKVGGLLLEIA
                     AEADTIHYGLLGIGIDANISLGDLPPNLKDTVTSLQAEVGHEVDRVALLARVLREFEL
                     RYQQLEEGEYDSIIREWKSLSLTLDQRVAIRTINKTFEGVAIDIDDHGALIIRKDNGR
                     IERVIAGDCFQL"
BASE COUNT         1366 a         2265 c         2279 g         1440 t
ORIGIN      
        1 gaaccgcttg aggaacatga tgtagtcgtg cagggtgttc tgcttgaacg gcctgccgcg
       61 ctggttcctg gcctctttga tggtggctat ggcagagaag atatcgccga tggtcgcttc
      121 ccggtagggg cagatgtaac ggcgccagtt gatcaggtgc gacgtgatct tgtttacccg
      181 gacggttgtg atcccttgcg acgcctggag ttccgtcacg aactcagtga tgagatcggc
      241 atcgtcgcgg gtgaggaggc ccgtggccac tgcttttcgg acggctgtgg tggcgtaggt
      301 tttatcgaca ggatggaact ctctgtcggt ttgggttggt gctgtgaacg ctcccatgca
      361 agggaggtcg gaatggaggt ataaaagcgt ttgctgacag atctgaacct gaataataga
      421 tgctgcgggt gggatccgaa cccacgatct ccagatgtcc caggtcctga cgctccgttc
      481 tctcaaaaac cctatgagtc tggcgccata accggctagg ccaccgcagc gcacaaagtg
      541 cattataaca atgatgtatt gacaaataaa ccttgtgcat tcgggaaaat tgagatggga
      601 ggtcactcca tgtttttccg gaggtagaca tcgaccgccg cggtgatcgc cgccaccttc
      661 ttcccctgcc ggagcgatgc ggccaacgtc tgcatacgtg tctcctcctc gcggaggtag
      721 cggcgcaggc tcgcggagat gtgctcctcc tgcaggaagt gcgtatgtgt gtcgccggcg
      781 acgaagtgcg ggtttcgcat gatcgcgtaa tggagcggga gtgtcgtctt cactcccagg
      841 atgacgtact cgtagatggc ccggcgcatc cgctggatcg cctcctcgcg gttggacccc
      901 caggcgcaga gtttcgcgat catcgagtcg tagtgcgggg gaatggcgta gcccacgtgg
      961 atgccggagt cgacccgtat accgggtccg cccggcgacc ggtaacggac gatcttgccc
     1021 ggatccgcgg tgaagttgtt cagcgggtcc tccgcgttga tccggcactc gatcgcgtgc
     1081 ccccggatgc tgatatcctc ctggcccatc gcgaggtcct cgccggcggc aacggcgatc
     1141 tgctgcttca cgatgtcgat accggtgatg aactccgtga tggtgtgctc cacctgcagc
     1201 cgggtgttca cctccatgaa gtagtagttg ccgtttgagt agaggaactc caccgtaccg
     1261 gcgttcctgt agttcgccgc ctttgccgcg gcgatggccg acgccgtcat ccgctcgcgg
     1321 agatccggcg tcatgatcgg acagggcgcc tcctcgagga gtttctggtg ccggcgctgg
     1381 atggagcact cccggtcgta gaggtggacg gtatgccctt ttgcatcggc aagaacctga
     1441 acctcgatat ggcgcggttt cgcgagatac ttctccacga agaccgtcgg gtccccgaag
     1501 gcggaaccgg cgatccgcat tcccccctcg attgcttcct caaggccctc ctcgctctcg
     1561 acgatatgca taccgatacc gccgccgccc gcgctcgcct tcacgaccac cgggtagccg
     1621 atagcggcgg cgacctcttt tgcctcgtct agacaggtga cgccctccgg cgtgccgggg
     1681 agaaccggaa cgcccgcctc gcgcatcatc cgcttcgacc cgatcttcga gcccagcgcc
     1741 tcgatcgtct tccacgaagg tccgatgaac gtccgcccct cgtcctcgac cagttttgca
     1801 aaccggtagt tctcggcgag gaacccgtat cccgggtgga tcgcttccgc cccggatttc
     1861 cgggcgatgt cgcagatccg ctccttgttc aggtaacttt tggaggggtg tgcctcgccg
     1921 acaaagaagg cctcgtcggc gtatttgacg tggagcgcgt tcttatcggg ctccgagtag
     1981 attgcaaccg tatcgatgcc gagctcccgg caggcgcgca taacccggat ggcgatctcg
     2041 ccgcggttgg cgatcaaaat tttgtcaaaa tatttcatat cggtgccggc ctcactcgac
     2101 gatcagcagc acgtcgccgt tctgcacgac gtccccggcg ttcacgaaga tctcttcgac
     2161 cctgccgtca cgcggactac ggatggggtt ctccatcttc atcgcctcaa ggacgatgag
     2221 cgtatcccct ttcttgacgg cattcccttt ctgggtcagc acctgcagga ccatgccctg
     2281 catgttgctc ttgacgccgc cggcgacatc tccgcggggg gctctcccct tgcccgccgg
     2341 agcggttgcc gaacccggaa ccgtgaccga actcccctcc acggtgacga tccggacaga
     2401 gaagatctcg ccgtcgacct ccacctccat ggagctcggt atctccgcta tccgcgccgc
     2461 cggagcggtg accggtttcg ggatcacctc ggcctgccgc tcgcctctga ggaacgacgg
     2521 agcgacgctc gggtagagga tgtaggtgag gacatcctca tcgcaggtga cgaggccctg
     2581 ttctgttgct tccttgcaca tctggccgca tatgggcgcg aggagatccg ccgggcgaac
     2641 cgtgaccggt tcttcgccgt cgatgatcag ctcccgtatc tcggggctga tcggtgccgg
     2701 cggccggccg tagaggccgc ggacgtaatc tttcacctcc gtcgtcacgt tccggtagcg
     2761 ttctccgtcc atgaggacgt tgaagaccgc ctgggtgccc acgatctggc tcgtcggcgt
     2821 cacgagcggc gggtaaccga gatccttcct cacccgcggg atctcctgga gaacctcctc
     2881 caggcggttg agtgcattct gctcttttaa ctgggagatg aggtttgata tcatgccgcc
     2941 cgggagctgg tagatcagaa catccgaatc gacccggtct gcgatggcgg aaaagagcgg
     3001 ttcgtatttc tcccgcatat cccggcagac gttgcgcaca ttccgtagcg gcagaagatc
     3061 gatgccggta tcgcgcggtg tcccgatgac gcttgcaacg acgctctccg tggggggttg
     3121 agatgtcccg agagcaaacg gcgacatagc cgtatcgaga atatccacac ccgcatcgat
     3181 cgccgcctgg taactcaggg gcgcaacgcc gctcgtgcaa tgggagtgga ggttgaccat
     3241 gacgtccatc gtctccttga tgccggatat caggtcgcgg gcatcgtgag gcatgatcag
     3301 gccggccata tccttgatgc agattgagtt gcacccaagc gagtagagtt ccccggccat
     3361 atcgatgaac gatgcgacgg agtgaaccgg gcttgtcgta taggatatcg cgccctgcag
     3421 gtgcgccccg acgttctgca cctcctccat cgccttcttc atattccgga tatcgttcaa
     3481 cgcatcaaag acccggaaga tatcgacacc gttccttgcc gatgcttcca cgaacttctc
     3541 cacgacatcg tcgggataat gccggtatcc cacgaggttc tggccccgca ggagcatctg
     3601 gattggggtg cgcttgagct cggccttcag catccggaga cgctcccagg ggtcgtcatt
     3661 caagaacctg atgcaactgt caaaggtcgc cccgccccag gcctcgaccg agaagaagcc
     3721 gacttcgtca atggcccggg caagagggag catatcctca gtccggagcc gggtggcgat
     3781 cagcgactga tgcgcatccc taagcgtggt atcggtgatg tggagtgtat caaacttggc
     3841 agcacacatc gaattttgca cctcgaaggg ctgaggagat agcgataaag cctctaaaaa
     3901 attcacccta ataatataaa aacatcacga acagggaagc aggataaaag cccgagtatt
     3961 accgccgata cggatgccag aatgtctcgc gggtccgttc tgaaccgggg gcatatccgc
     4021 cctccgagcg cgtatccccg tactaccagc agtctggcga cttcgtcggc cctccggatc
     4081 tgggtgacga tgagcagaac ggcgatcggc acgatcgagc gtgcgccgat cgggatcccc
     4141 ttgagcgcaa gtgcgacccg cgcctggtcg atctcctgcc ggatcacgag gagcccggta
     4201 agccccatct cggcgatgag cccgatctca aatccgatcc gcttcccgag cgcccagacc
     4261 gccacagcaa gcacttctcc ctcctccgtc tcggtatatg cccacgcggc aaggaggagg
     4321 accacgacca tccggatgaa gtaggagagg ccggggccgc ccccccatgc cgagacgagt
     4381 gcggttaggg cgatcatagc ggtgagggag aagagtactc cgggccgtgg gagagctttt
     4441 gttcgcggcg tgaagacgag ccaccagacc agggcgacga cggcgcccat cgtgcttgca
     4501 aacgcggcaa gcgagagaac cacggtggag agaagcctca gccttggatc ctgcatatcg
     4561 cctcccgtgc atcctcaagc ctgatgttct ccgggcgggc gccccggtcg agggcatacg
     4621 caaggtaggg tggggcgtgc cgccaccgcg ggattgcgcc gggcacgttg ccgagggcgg
     4681 tcaggatccc gccctccatc tcccagatgg cgtccacccg ggggagaacc gcgcgctcgt
     4741 gcgagaagat gatggtgatc ccccgccccc tctcctcgat catcctgcag gccttctgtt
     4801 tcgcggcaca atcaagcgaa ctgaacggtt cgtccagcat cagcaggtcc ggatcccgca
     4861 tgaacgtgca ggccaggatc aaccgcttca gttcaccgcg ggagaggtgg aacgggtcgt
     4921 cgtcgccgcg gtcggcgaga tccgcccggg cgagcacatc gtccggcact aacccccagg
     4981 atccggcctc ttcggcaacg gtcggcgagg tgacgtgata ttcgggaaat tgcagcagaa
     5041 gcagggtcga tgagaccctc tgccgtcgga tcgcaccgct ccccggatgg aggagcccgg
     5101 caagcaggag ggcgagcgtc gatttcccgc tccccaccgc tccgctgacc aggtgcacgc
     5161 cctcctcgaa ggtgccgctg ccccggaggg tgaacgggcc gcgagagagg agaagatcct
     5221 caagttccac cctcaccggc ggatcctcca catcgtgggg tagaaacacg tctcctcgag
     5281 gctcgtaaaa acctcctcgg gagtgccgtg gtgccggacc gccccccctg ccatgaagac
     5341 gacataatct gcttcggccg cggtgtccat cgactgcgtg caccagagca catgggccgc
     5401 cgtgctcttc ttcacggcgg cctgtatgcg cgcggccgtc ccgccgtcca ggtgcgagtc
     5461 ggcctcgtcc agtatgagca cctcgggatc gtcgacgacg gccgcagcca gcgcgacgag
     5521 ggctttctcg cctcccgaga gggccgatac cttcgcatcg aggaggtgcg ggatgccgat
     5581 cgtcccggcg gccgcccgga cacgttcgtc ggtttcgagg caggggcggt ggcggaaccg
     5641 gggagacgat gcaatctcgt cgtatgcccg ggagaagagg agcgtccggt cgggaaactc
     5701 tcccacccac ccgatcttca cggcggcggg cgccctcccg aggagccgga ccgttcccct
     5761 tcggggctct tcgataccgg agcagatcga gaggagcgtt gtcttcccac tcccgttcgg
     5821 cccgatgacg gcgacatgcc gtgcatccac cgtcagatcc ggaatgtcga cgatccggtg
     5881 ccggaggttg acgatccgga tcatgccacc cgttttccta tggtatatgc ggcggctgcc
     5941 ttcagggtat caccgacgat gaatggagcg acaccgatca agatcgcctg gagaagcggg
     6001 atcgaggcag agtatgagag ccatacggcg ccggccgaga ggtagattgc acaggccgca
     6061 atcaggcctc cgatacgcca tttcggtgat tcctgctcat aggcgagccc ggcgatgagg
     6121 gcggcgggga taaaaccgat caggaaccct cccgtggggc cgaggaggac accgacgcct
     6181 gccgtgccgt tatgaaagac cgggaggctc atcgccccga ggaggacgta gagggcgacg
     6241 gggatcaccg cgtagcgttt catgacgatc ccggcgagga gcacaaagag ggtctggagc
     6301 gtgatcggaa ccgggggcag cggtatggat atccagctcc cggcagcgat gagcgcaacg
     6361 aatgtgccgc tgtaagcaag gatctgggcg cggtgtttca tgtaaaccat cttattttag
     6421 atggttgaca ataaaaatca tgtcaaccca aaagttatgg gagccggcat gacccggaac
     6481 gctagagctg gaagcagtcg ccggcgataa cccgctctat tctcccgtta tccttgcgga
     6541 tgatcagggc gccgtggtcg tcgatatcga tcgccacccc ttcgaaggtc ttgttgatgg
     6601 tcctgatggc cacccgctga tcgagcgtca gagagagact cttccactca cggatgatgg
     6661 agtcatactc gccctcctcg agctgctggt agcgcagttc gaactcccgc aggacccggg
     6721 caagaagcgc cacgcggtcg acctcgtgac cgacctccgc ctggagcgag gtcaccgtat
     6781 ccttcaggtt cggcgggaga tcaccgagcg atatgttggc gtcgatccct atgccgagga
     6841 ggccgtaatg gatggtatcc gcctccgcgg caatctccag gagcagcccg ccgaccttct
     6901 tatccccggc gaatatgtcg ttgggccact tgatgagtgc gctgatgttg tactctttcc
     6961 tgatggcgcg ggcgattgcg atcgccccgg ccatggtgat catgaagaga tgatcgagcg
     7021 gtatcttcgg tttcaggacg atggtgatcc atatgccgcc ggcaggcgag acccaggcgc
     7081 gcccgagtct gccgaccccg ccggtctgct cttcggcgat gatcaccatg ccgtgcagct
     7141 tctccggatc ggtctcggcg gcaagttgct ttgcgatcca gctcgtcgat gcggtgcgat
     7201 cgaagtggcg gatctgtctt ccgatggacc gggtgcggag ttttttgtgg atctcatacg
     7261 ggaggagttt gccggtcgct gactcaaggc gatacccctc cgcgcgggac gatgcgatgc
     7321 cgtatcccaa ccgtcggagt tcccgcacct
//
LOCUS       DAOSII010000095         7226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_9422_length_7226_cov_49.508716, whole genome shotgun sequence.
ACCESSION   DAOSII010000095 DAOSII010000000
VERSION     DAOSII010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9422_length_7226_cov_49.508716"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PLV90_07665"
     CDS             <1..462
                     /locus_tag="PLV90_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOF97029.1"
                     /translation="REVQRTVLEMILRGDAFEGVQAYLRDVIRKYRRGEYSLDEVGIP
                     GGIGKNLDSYETDDAHIRGARYANDHLGTDFKRGSKPKRIYIKTVTAKYPRTDVIAFE
                     YADQVPPEFVVDWETMLEKTLKGPISRIIEPLGWDWHDVDPTRTTLFDFGM"
     gene            complement(487..918)
                     /locus_tag="PLV90_07670"
     CDS             complement(487..918)
                     /locus_tag="PLV90_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97030.1"
                     /translation="MGGVKDSTYLDVKKALARRFSPQEGWQFAWYPTYGNVQPECVLS
                     RRTAGRTERVVVGVKMASKVPVGTIEELQGQRQALAASNVDIDRAVLVVPGGASVPAV
                     PEGIEILEMGNWQIVGDRIAWSKNIERSAFHQEERVKRGLA"
     gene            1144..1770
                     /locus_tag="PLV90_07675"
     CDS             1144..1770
                     /locus_tag="PLV90_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97031.1"
                     /translation="MAEATEIGVVEAYERLAREVLNRPESIPSAIHNLLLKLAETHPG
                     AVYWIVRKFYQEYLRLYVSDTGYIGIADRYEPGLMYPGDINLYMVPESPQPGDVVQIT
                     FTDKNEVSIYVIHGRVLRCNEDRSVQVEDTSTGEDYRVVDWNILGKLVKVIPFGSDEW
                     QELFPASGIPKEWLTTSVEGNIRSLQESDASGKDEAIAELKRRLRELV"
     gene            1887..2867
                     /locus_tag="PLV90_07680"
     CDS             1887..2867
                     /locus_tag="PLV90_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97032.1"
                     /translation="MRHLIVIVLILLLIPSGVQALTFLSGDQPAVDTPIPDDVVASGG
                     IVTINAPIDSLTAAGGTVTVNAPVKNDVIAAGGTLIINGDVGGKVLAAGGDIEVNGNA
                     TNVLATGGTVRIGQGAVIRRDAVLSGGTVANEGSVVRNLTVSANNFSNTGTAGNVTFE
                     EREEPGPLLAGIFSVLAAIGFFILGLLLVGLLPGPFSTVVHEVERRPVVFTVLGFIAL
                     FVTTIVLLIVAVTIIGLPIALFGGLLFIVGMLVSPLFVAYALGDVIASRAGRRPGRIW
                     IFVLGFVILQILIFIPLFGMIVHAVAVSLGYGGLLSALRSIRQSRAGEAA"
     gene            complement(2899..4377)
                     /locus_tag="PLV90_07685"
     CDS             complement(2899..4377)
                     /locus_tag="PLV90_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOF97033.1"
                     /translation="MSERDILLALERRAGEKPEFRLSELQKDLAAGQSLEDLAGEVRP
                     QIDRLGLTLRPSGGDYVIGRRAGPLTVDDAGRERQAAFFRNPEVPETIQHLIESYITR
                     KTGKPWDDPAVLDRMQNAILVQKSRYWKEQRVSYRKAYQVLGYLAYHAPVYLVQFEHI
                     FWQIIDRGLAKPHMRILDIGTGPGVVPLAVIDLLDRIGSASAEVYAVERSEEHLEAYN
                     ALVPAAAAAMGDRVQVKKPTRGDIGEIAAGDLPDRIDIMVFSNVLGELRHMRIEERAD
                     LVAALAEQLAPDGTIVVVEPADLANATALRQTVRAIADRGLTIHSPCSFIRGTACNPA
                     RCWTFEQKGDIRPPRLMERLAEGKDGYRFVNVDIKYSSALLRKDARVQHAYRVPPGAK
                     FARFSHLHRHVDRTINVVAALMSGNLGDDRTKVYKVCDGTPADPVYVVVPATLRGQNR
                     DALRDIAYGDIVRFHGVLVRFNKDHGAYNLVVLPGTRVEPAA"
     gene            complement(4445..5035)
                     /locus_tag="PLV90_07690"
     CDS             complement(4445..5035)
                     /locus_tag="PLV90_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOF97034.1"
                     /translation="MKPSFDVLIVGIGGQGTVLASNVLGEACIIENRTVRSAETHGMA
                     QRGGSVESHIRIDGELGSLIVPGTADLLIAFDLLEALRYRHYLPAGGKLVVNRHLVVP
                     TSVYQQNLDVPDEESILAALADLDVACIDAAGLAAEAGSILAQNIVMLGAASRHIPLR
                     PETLEEAVRRCVPKKTVEVNTAAFRLGREAGARGTP"
     gene            complement(5032..6801)
                     /gene="iorA"
                     /locus_tag="PLV90_07695"
     CDS             complement(5032..6801)
                     /gene="iorA"
                     /locus_tag="PLV90_07695"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOF97035.1"
                     /translation="MAVRYLLGNEGIAHACLEANVDFASGYPGTPSSEVIDILRVQED
                     RPFTVEWSTNEKVAFENALAAAWCGLRALCTMKHVGLNVAADPLMTSAYTGVTGGFVV
                     LSADDPFAHSSQNEQDTRRYAHFARLPCLDPSSVQEAHDMLRDAFALSEEFGLPVIFR
                     PTTRICHSKSDVALGEIGTEHRTAAFHRDPRQYVVIPAHTRVLHKKLNEKQEALKERL
                     VELGYNRHAVRGRTAVIASGVAAAYVQEVLPDDVSLAIVGAYPIDEEWLREFVDRHEK
                     VLVLEELSPVVEEAVRQVATRTEVVGKLTGTVPYEGEFTPATVAAALAWAGIAPTIAF
                     PAVAPVQGVPPRPPILCAGCMHRPTFYAMKKVFRDGIFPSDIGCYTLGLQLGAVDTTI
                     CMGASITVGSGIARSGETHPVVSTIGDSTFLHTGIPGLLNAVYNDADMVVVILDNRIT
                     AMTGHQPNPNTGVTATGEESTPISLEAICRSCGVSWVETVDPYDLPLLLDTFRRAKER
                     RGVRVIIAKQPCVITARRSGIKRKAYAVDPERCTGCGVCRSFGCPAIAFSEKNATITD
                     LCAGCGVCADICPSGAIGMEGRQ"
     gene            6908..6980
                     /locus_tag="PLV90_07700"
     tRNA            6908..6980
                     /locus_tag="PLV90_07700"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6941..6943,aa:Ile,seq:gat)
BASE COUNT         1206 a         2258 c         2418 g         1344 t
ORIGIN      
        1 cgggaggtgc agcgcacggt ccttgagatg atcctccgcg gggatgcgtt cgagggcgtg
       61 caggcctacc tgcgcgacgt catccggaag taccggcgcg gcgagtactc cctcgacgag
      121 gtcggtattc ccggcggtat agggaagaac ctggacagct acgagaccga cgatgcccac
      181 atccggggcg ccaggtacgc aaacgaccat ctcgggaccg acttcaagcg gggcagcaaa
      241 cccaagcgca tctacataaa gaccgtcacg gcgaagtacc cgcgcacaga cgtgatcgcc
      301 ttcgagtacg ccgatcaggt gccgccggag ttcgtcgtcg actgggagac gatgctcgaa
      361 aagaccctca aaggacccat atcgcggatc atcgagccgc tcgggtggga ctggcacgac
      421 gtcgacccga cacggacgac gctctttgat ttcggtatgt aagaaaagtt ctggggggcc
      481 ggcgctttac gcgaggcccc ttttcacgcg ctcttcctgg tggaacgcgc tgcgctcgat
      541 attctttgac caggcgatgc ggtcgccgac gatctgccag tttcccatct cgaggatctc
      601 gatcccctcg ggaaccgccg ggacgctcgc cccgccgggc acgaccagaa ccgccctgtc
      661 gatgtcgacg ttgcttgcgg caagcgcctg gcgctggccc tgaagttcct cgatggtgcc
      721 caccgggact ttggatgcca tcttcacgcc cacgaccacc cgctcggtcc tgccggctgt
      781 ccggcgagag agcacacact ccggctgcac attgccgtag gtgggatacc atgcaaattg
      841 ccacccttcc tggggagaaa accgccgggc gagggctttc ttgacatcga gatacgtaga
      901 atcctttacg ccacccatat caatatcctc tgctttactg tatggtcacg gtcgaatata
      961 agcattatag ttctctcctt cccggcgccc cgcgaggtgc ccgggacggc ggcaggcgac
     1021 gttccccttc ccggggccgg tgcagggacg ccgggcgcaa aggccgggcc ggatgccggc
     1081 ccggaataat ccggaggaac acttttattc tattaccggt attatgcggc ggggtttgtc
     1141 agcatggcag aggcgactga gatcggagtg gtagaggcgt atgagcggct ggcgcgcgag
     1201 gtgctgaatc ggccggagag cataccgagc gccatccaca acctcctcct gaaactggcc
     1261 gagacccatc ccggggcggt ctactggatc gtccggaagt tctaccagga gtacctccgg
     1321 ctctacgtct ccgataccgg gtacatcggt atcgcggaca ggtacgagcc gggcctgatg
     1381 tacccgggcg acatcaacct ctatatggtg cccgagagcc cgcagccggg cgacgtggtc
     1441 cagatcacct tcaccgacaa gaacgaggtg agcatctacg tcatccacgg ccgggttctc
     1501 aggtgcaacg aggaccggtc ggtccaggtc gaggatacca gcaccgggga ggactaccgg
     1561 gtcgtcgact ggaacatcct cggcaaactg gtcaaggtca tcccgttcgg gtccgacgag
     1621 tggcaggagc tcttcccggc gtccggcata ccgaaggagt ggcttaccac gtcggtcgag
     1681 gggaacatcc gctcgctcca ggagtcggac gcttccggga aggacgaggc gatcgcagag
     1741 ctcaaacgcc gcctccggga actggtgtag gagcagaggc tctaagacgc ctcttcttgc
     1801 tgccggaaag gtaattccgg gttctcctcc agccggtctc ttcatcacgc gaatcttttt
     1861 aaatgggggt gtgctctcct ccccccatga gacaccttat cgtgatcgtc cttatcctgc
     1921 tgctcatacc gtccggcgtt caggcgctga cgtttctcag cggcgatcaa cccgcggttg
     1981 atacccccat acccgatgat gtggtagcat cgggagggat cgtgaccatc aacgccccga
     2041 tcgatagcct gacggccgcc gggggcacgg tgacggtgaa cgcgccggtc aaaaacgacg
     2101 tcatcgccgc gggagggacc ctgatcatca acggcgacgt cggcgggaag gtgcttgccg
     2161 ccgggggcga tatcgaggtg aacgggaacg caacgaacgt tctcgccacg ggaggcacgg
     2221 tgcggatcgg gcagggcgcg gtcatccggc gggatgcggt gctcagcggc gggacggtcg
     2281 cgaacgaggg atcggtcgtg cgtaacctca ccgtctcggc aaacaacttc agtaataccg
     2341 gcaccgccgg gaacgtgacg ttcgaggagc gcgaggagcc ggggccgctg ctggccggca
     2401 tattttcggt gcttgccgcg atcgggttct tcatcctcgg cctcctcctc gtcgggctcc
     2461 ttcccggccc gttctcgacg gtggtccacg aggtcgagag acgcccggtc gtcttcacgg
     2521 ttctcgggtt catcgctctc ttcgtgacga cgatcgtcct cctgatcgtc gccgtcacca
     2581 tcatcgggct ccccatagcg ctctttggcg ggttgctctt catcgtcggc atgctggtat
     2641 cgccgctctt cgtcgcgtac gccctcgggg acgtcatcgc ctcgcgggca gggcggaggc
     2701 cgggacgcat ctggatattc gtcctcgggt tcgtgatctt gcagatcctc atcttcatac
     2761 cgctgttcgg gatgatcgtc catgccgtcg cggtcagcct cggctacggc gggctgctct
     2821 ccgcgctcag gagcatccgc cagtcccgtg caggcgaggc tgcgtgatcg gccggcgagg
     2881 gttccggggg gagagggcct atgcggccgg ctctacccgg gtccccggca ggacgaccag
     2941 gttgtatgcg ccgtgatcct tgttgaaccg gacgaggacc ccgtggaacc ggacgatatc
     3001 accgtaggcg atatcgcgga gcgcatcgcg gttctgcccg cggagggtcg cggggaccac
     3061 gacgtagacc ggatccgcgg gcgttccgtc gcagaccttg tagaccttcg tccggtcgtc
     3121 gccgaggttc cccgacatca gggcggcgac gacgttgatc gttctatcga catgccggtg
     3181 gaggtgggag aaacgggcga acttcgcccc cggcgggaca cggtaggcat gctgcacccg
     3241 ggcatccttt cgcaggagcg ccgaagagta cttgatatcg acgttcacga accggtagcc
     3301 gtcttttccc tccgcgagcc gctccatcag acggggcggc cggatgtcgc ccttctgctc
     3361 gaatgtccag caccgcgcgg ggttgcaggc ggtgccccgg atgaacgagc aggggctgtg
     3421 gatggtaagc ccccggtcgg cgatcgcgcg cacggtctgc ctgagagcgg tcgcgtttgc
     3481 aaggtcggcg ggctccacga cgacgatcgt cccgtcgggc gcgagttgtt cggcaagggc
     3541 ggcgacgagg tcggcacgct cctctatcct catgtggcgg agttcgccaa ggacgtttga
     3601 gaagaccatg atatcgatcc ggtcgggcag atccccggcc gcgatctccc cgatatcgcc
     3661 ccgggtgggt ttttttacct gcaccctgtc tcccatcgcc gcggccgccg ccgggacgag
     3721 ggcgttgtac gcctcaaggt gctcctccga gcgctcgacg gcgtagacct ccgctgaggc
     3781 gctcccgatt cgatcgagga gatcgatgac ggcgagcggc acgacccccg gcccggtgcc
     3841 gatatcgagt atgcgcatgt ggggttttgc gagcccccgg tcgatgatct gccagaagat
     3901 gtgctcgaac tgcaccaggt agacgggcgc gtggtaggcg aggtagccga ggacctggta
     3961 ggccttccgg tagctcaccc gctgctcctt ccagtatcgg ctcttctgga cgaggatggc
     4021 gttttgcatc cggtcgagga cggccgggtc gtcccacggc ttccccgtct tcctcgttat
     4081 gtaagactcg atcaggtgct ggatggtctc gggcacctca gggttccgga agaacgcggc
     4141 ctggcgttcg cgcccggcat cgtcgaccgt gagcggtcct gcccggcggc cgatcacgta
     4201 gtcgccgcct gaaggcctga gggtgaggcc gaggcggtcg atctgcgggc ggacctcccc
     4261 ggcaaggtct tcgagcgact gcccggccgc caggtctttc tgcagttcgg agagccggaa
     4321 ctccggcttt tcgcccgccc gccgttccag cgccagaaga atatcccgct cgctcatggc
     4381 gtatcaggtc ggcggggcgg gtaatacagg ttgcgggcaa aaatgatgag ggggggctct
     4441 cccgtcaggg cgtgccgcga gcccccgcct cccgcccgag ccggaaggcc gcggtgttga
     4501 cctcgacggt ctttttcggg acgcagcgcc gcaccgcctc ttcgagggtc tcgggccgga
     4561 gcggtatatg ccgggacgca gcgccgagca tgacgatgtt ctgcgcaagg atgctccccg
     4621 cctccgccgc gaggcctgcc gcgtcgatgc aggcgacgtc gaggtcggca agcgcggcaa
     4681 ggatgctctc ctcgtcgggt acgtccaggt tctgctggta gaccgatgtc gggacgacga
     4741 ggtgccggtt cacgacgagt ttcccgccgg ccgggagata gtggcggtaa cggagggcct
     4801 cgaggaggtc gaaggcgatg agaaggtcgg ccgtcccggg cacgatcagg gacccgagtt
     4861 ccccgtcgat gcggatatgg ctctccaccg atcccccgcg ctgtgccatg ccgtgggtct
     4921 cggcgctccg gacggtccgg ttctcgatga tgcacgcctc cccgaggacg tttgaggcga
     4981 ggacggttcc ctggccgccg ataccgacga tcaggacgtc aaacgagggt ttcattgtcg
     5041 cccctccatg ccgatcgctc ccgacgggca tatatccgcg cagacgccgc acccggcgca
     5101 gaggtcggtg atcgtcgcgt tcttttcgga gaacgcgatt gcggggcagc cgaacgaccg
     5161 gcagacgccg cagccggtgc accgctccgg gtcgaccgcg taggctttgc gtttgatccc
     5221 gctcctccgc gcggtgatga cgcagggctg ctttgcgatg atgacgcgga cacccctgcg
     5281 ctcttttgcc cggcggaagg tgtcgaggag gaggggcagg tcgtaggggt cgacggtctc
     5341 gacccaggag acgccgcagg accggcagat cgcttcaagc gatatggggg tgctctcttc
     5401 tccggttgcg gtcacgccgg tgttggggtt tggctggtgg ccggtcatcg cggtgatccg
     5461 gttgtcgagt atgacgacga ccatgtctgc gtcgttgtag acggcgttca ggaggcccgg
     5521 tataccggtg tggaggaacg tcgagtcgcc gatggtcgag accaccggat gcgtctcccc
     5581 cgatcgggcg atgccgctcc cgacggtgat cgaggcgccc atgcagatgg tggtatcgac
     5641 cgccccgagc tggaggccga gggtgtagca cccgatgtcg cttgggaata tgccgtcgcg
     5701 aaagaccttc ttcatggcat agaacgtcgg ccggtgcatg cacccggcgc agaggatagg
     5761 cggccggggc ggcaccccct ggaccggtgc gaccgcaggg aaggcgatcg tgggtgcgat
     5821 gcctgcccac gcaagagcgg ccgcgacggt tgccggggtg aactctccct cgtacggcac
     5881 cgtgccggtc agcttcccga cgacctccgt tctcgtcgca acctggcgaa ccgcctcttc
     5941 gacgaccggg gagagttcct cgaggacgag gaccttttcg tgccggtcga cgaactctct
     6001 gagccactcc tcgtcgatcg ggtaggcgcc gacgatcgca agcgagacgt cgtcggggag
     6061 gacctcctgg acgtaggcgg ccgcaacgcc gcttgcgatg acggcggtcc tgccccggac
     6121 ggcgtggcgg ttgtagccga gttcgacgag cctctctttg agcgcttcct gcttctcgtt
     6181 cagcttcttg tgcaggaccc gcgtgtgcgc ggggatgacc acgtactgcc gggggtcgcg
     6241 gtggaaggcg gcggtccggt gttcggtgcc gatctccccg agggcgacgt cgctctttga
     6301 gtggcatatc cgggtggtgg gccggaagat caccgggagg ccgaactcct cggagagggc
     6361 gaaggcgtcc cgcagcatgt cgtgcgcctc ctgtaccgac gaggggtcga ggcaggggag
     6421 ccgggcaaag tgcgcgtagc gccgggtgtc ctgctcgttc tgggagctgt gcgcgaaggg
     6481 atcgtctgcg gagaggacga cgaacccgcc ggtcaccccg gtgtatgcgc tcgtcatcag
     6541 gggatcggcc gcgacgttca gcccgacgtg cttcatggtg cagagtgccc ggagaccgca
     6601 ccatgcagcg gcgagcgcgt tctcaaaggc gaccttctcg ttcgtggacc actccacggt
     6661 gaaggggcgg tcctcctgca cccggaggat atcgatcacc tctgaggacg gcgttcccgg
     6721 gtagccgctg gcaaaatcga cgtttgcctc aaggcatgcg tgagcgatgc cctcattccc
     6781 gagtaagtat cgtacagcca ttgttcaact caactatact tctatcatcg gttttcaaca
     6841 tagcgctttg gatgcacgcc aaaacaatta agtgctatta tggcggatat tgtagggtag
     6901 tcctattggg cccgtagcat agccggtggt gcacccggct gataaccggg aggtcatgcg
     6961 ttcgaatcgc atcgggccca tcgttctttt catcaaagcg cactggattc cagcgtagct
     7021 caccgatgcc cttctccatg ccgcttttct ccgtaactcc agttctacgc cggggtttca
     7081 aaaaagaatg gtggtacgtg agcgcacccg cccggcctcg atggcgacag ccgccacggt
     7141 caggtgcgag gcgagagccg gggaaggagc cggccacacg gttgcccacc ggatatcgcc
     7201 ctgccttccc gggagagaga tgggcg
//
LOCUS       DAOSII010000096         7063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_9678_length_7063_cov_56.566210, whole genome shotgun sequence.
ACCESSION   DAOSII010000096 DAOSII010000000
VERSION     DAOSII010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7063
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9678_length_7063_cov_56.566210"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PLV90_07705"
     CDS             <1..360
                     /locus_tag="PLV90_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF97036.1"
                     /translation="EGNTLVLTTHYLDEAERLCDRLVIMDHAKILVEGAPADLIREHA
                     GSNVVEAERTPAVLTCLDEFGLDYDLAGGAIQVFTDHPHDVARELLETCRHEAVTVRP
                     ATLEDVFLRLTGRSLRE"
     gene            368..1135
                     /locus_tag="PLV90_07710"
     CDS             368..1135
                     /locus_tag="PLV90_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF97037.1"
                     /translation="MIDITGRVWSVWRRNRDAFLKTYRVNFIPPFVEPVLYLLALGFG
                     LGTYIESVDGIPYPVFIAPALVSISVMYSSFFECTYSSFVRMYYQKTFDAIIATPVSI
                     DEVIAGEMFWGATRGMIYAALMLPVLLLFGVVAMPSSLLLIPFAYLAGLLFATIGMCF
                     TAITPGIDALNYPAFLFITPMFLFSGTFFPLDLLPEPIRYFALAVLPLTHVVSINRAI
                     TLPAFAPANLISLAWIAVATAFFFVLAIRLMRRRLIV"
     gene            complement(1256..1432)
                     /locus_tag="PLV90_07715"
     CDS             complement(1256..1432)
                     /locus_tag="PLV90_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97038.1"
                     /translation="MEERYPSRGPRSFQDRPREFHKAVCSDCGKACEVPFKPTEGRPV
                     YCRDCLPKHRKPRF"
     gene            complement(1475..1651)
                     /locus_tag="PLV90_07720"
     CDS             complement(1475..1651)
                     /locus_tag="PLV90_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97039.1"
                     /translation="MDERYSSRGPRSFQDRPREFHKAVCSDCGKACEVPFKPTEGRPV
                     YCRDCLPKHRKPRY"
     gene            1973..3964
                     /locus_tag="PLV90_07725"
     CDS             1973..3964
                     /locus_tag="PLV90_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOF97040.1"
                     /translation="MTDEIHTDEPPDSRRKGRTRDKRTIGPVPNLEEHVKSDWWRRIF
                     NHLYLKTDGDVVDDQRITGREIDRVARVLRLGPDEKILDLCCGQGRHTLELARRGYNA
                     EGLDQSHYLIQRARSTAKREGLQVRFREGDARRLPYRTDSYDVVLVLGNSFGYFDSVE
                     EDLRILTELRRILKPWGQVLLDVADGEYLKEHFQPRSWEWIDRTMFVCRERSLSLDGQ
                     RLISREVITDVDKGVVADQFYAERLYTRDALRQLLERAGFSRIAFHEIATESQRNQDL
                     GMMERRHIVTAAIRKEWAKAKAKGQERAKHVAVMLGDPTKADALKPSCVFDDDDFYTI
                     DQMKAALRELKGYRFTYLCKHDTMIPDLTKLKGKVDYIFNLCDEGFNNDPRKELHVPA
                     LLEMLDIPYTGSAPQSLAFCYDKSLVRGIAKEMGIPVPDACFITPGDRTYDVGMKFPV
                     IVKPNSGDSSYGITQRSIAHTIEELSDIISSLRTTLGYSQSLLVEEFLTGKDISVGII
                     GNPSGYYTVLPITEEDYSALPPELPRICGYEAKWLPDSPYWKIVSRPANLPEETEKVV
                     VRCCLALFERLECRDYCRFDWRLDEHGNPKLLEVNPNPGWCWDGHLAKMAKYAGLSYP
                     DMLGRVLKAAEERFGMDQETGAREAEAKTVPLDLSRQEA"
     gene            4092..5006
                     /gene="nifB"
                     /locus_tag="PLV90_07730"
     CDS             4092..5006
                     /gene="nifB"
                     /locus_tag="PLV90_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase cofactor biosynthesis protein NifB"
                     /protein_id="HOF97041.1"
                     /translation="MADESYRTATVQGREVPYDPEQLRKISEHPCYSDKACHAFGRCH
                     LPVAPKCNIQCNYCVRDFDCVNESRPGVTSKILTPEEGLDLVRKVVKDYPFVKVIGIA
                     GPGEPLANPETFEALRLVHEEFPHLIMCLSTNGLMLPESIDELAKYDVGNVTVTLSAI
                     DPAIGEKIYSWVEYKGKKYRGREAAELLLSQQLKGIEMAVAKKMFVKINTVYIPGIND
                     EHIPAIAKKVGEMGAFTFNVIPLIPQYKFADITPPTAKDKREMQDRCAPFIKQMRHCA
                     RCRADAIGKLGQDVQSCVYQQMKDEKAE"
     gene            5028..5885
                     /locus_tag="PLV90_07735"
     CDS             5028..5885
                     /locus_tag="PLV90_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HOF97042.1"
                     /translation="MRTIELRPDQPLDLDLTLSCGQAFRWENVDGWWQGVVDGRAVRI
                     RQDADRLTFEGADAAFVRDYLRLDQDLPAILASIDRDPVIGAAVRECRGLRLVRQQPW
                     ECLASYICATNTNIPAVKRRVALMAERYGRAIDGPSGMTYAFPEPEALAEASRTDIWD
                     CKLGYRTDYICEAARFASENPGWAERIAALPFEDARRALMEFKGVGPKAADCVLLFAF
                     GFFEAFPVDVWIRRIVGETYLSDLAGRSCTPAEYERIRRFSRDYFGEYAGYAQEYLYC
                     ARGPARRQR"
     gene            complement(5889..6053)
                     /locus_tag="PLV90_07740"
     CDS             complement(5889..6053)
                     /locus_tag="PLV90_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97043.1"
                     /translation="MGEVAIATASPMIAVCGVPIGLAGIGTFALIGAVIFVLLAAAGL
                     TRQRVRSRKG"
BASE COUNT         1467 a         2159 c         2084 g         1353 t
ORIGIN      
        1 gaaggaaaca ccctcgtcct caccacgcac tacctggacg aagcggaacg tctctgcgac
       61 cgtctggtga tcatggatca cgctaagatc ctcgtcgagg gtgccccggc ggacctcatc
      121 cgagagcacg ccgggagcaa cgtcgtcgaa gcggaacgga caccggcggt gcttacctgc
      181 ctcgatgagt tcggtctcga ctacgacctt gccggcggtg caatccaggt cttcacagat
      241 caccctcacg atgtggcccg cgaactgctc gagacctgcc ggcacgaggc ggtcacggtt
      301 cgcccggcaa ccctcgaaga tgtcttcctc cggttgaccg gccggagcct gcgggagtga
      361 ggagaggatg atcgatatca ccggcagggt atggagcgtc tggcggagga accgggacgc
      421 cttcctcaaa acctaccggg tgaacttcat cccgcccttc gtcgagccgg tcctctacct
      481 cctggccctg gggttcggcc tcgggacgta catcgagtcc gtcgacggga taccctaccc
      541 cgtcttcata gcgcccgcgc tcgtctcgat atcggtgatg tactcgtcct tcttcgagtg
      601 cacctactcg tcgttcgtac ggatgtacta ccagaagacg tttgacgcca tcatcgcgac
      661 accggtcagc atcgatgagg tgatcgcggg ggagatgttc tggggcgcca cgcggggtat
      721 gatctacgcg gcgctgatgc tcccggtgct cctcctcttc ggcgtcgtgg ccatgccctc
      781 gtccctcctc ctcatcccct ttgcatacct tgccggcctc ctcttcgcaa cgatcgggat
      841 gtgcttcacg gcgatcacgc cgggcatcga tgcgctgaac tatcctgcgt tcctcttcat
      901 cacgccgatg ttcctcttct cggggacgtt ttttcccctc gacctgctcc ccgaacctat
      961 ccggtacttc gcccttgccg tgctcccgct cacgcacgtc gtcagcatca accgggcgat
     1021 caccctcccg gcattcgcgc cggcaaacct catcagcctc gcctggatcg cggtcgccac
     1081 cgcgttcttc ttcgtcctcg ccatcaggct gatgaggaga cggctgatcg tgtgacgggc
     1141 gggttccggt tcacccgggt tctcaggggc gtatgggact catataaagc cggcacgacg
     1201 gggggagggt ccgggcaacc cgccctgtat cgggtatgca ggcacaaaaa aagagttaga
     1261 atctgggttt tctgtgcttc gggaggcagt cgcggcaata gacgggtctg ccctcggtcg
     1321 gcttgaaagg aacttcgcac gctttgccgc agtcggaaca gacggcctta tggaactcgc
     1381 gggggcggtc ctggaacgag cgggggcccc ggctcggata tctctcttcc attggaattt
     1441 cactcacgtt gagaaaccgt actcactaaa cgactcaata ccggggtttt ctgtgcttcg
     1501 ggaggcagtc gcggcaatag acgggtctgc cctcggtcgg cttgaaagga acttcgcacg
     1561 ctttgccgca gtcggaacag acggccttat ggaactcgcg ggggcggtcc tggaacgagc
     1621 gggggcccct gcttgaatat ctctcatcca tatacttcaa ccatgcagat agtctgcata
     1681 tgtacaacta cctgccgcca tatataagtg cgtttcgtgg aatccggcgg gttccgtcac
     1741 cccccggtat accccgctcc gctgtcccgg cgcgtggggc gaaactttcg aggcatagaa
     1801 atccccgata atgcggcagt tcattccggt agagagactc atggcacaat gcttttatca
     1861 ataccgtcct accgaatgat cacccagcta tggtacacgc aatcaatctc ttttgtaatc
     1921 ggatgaaatg ccgaatctgt tccggagata ggaccaggat gagtggctca gcatgaccga
     1981 cgagatccac acggacgaac cccctgatag caggcggaag gggagaaccc gggataaacg
     2041 gacgatcggt cctgtcccga accttgaaga gcacgtaaaa tccgattggt ggaggcgtat
     2101 cttcaatcac ctctacctga agaccgatgg cgacgtcgtc gacgaccaga gaatcaccgg
     2161 gagggagatc gaccgggtcg cccgggttct gcgcctcggc cccgacgaga agatcctcga
     2221 tctctgctgc ggccagggcc ggcataccct cgaactcgca cgcaggggct acaacgccga
     2281 aggcctcgac cagtcgcatt acctgatcca gcgagcccgg tcgaccgcga agcgggaggg
     2341 gttgcaggtc aggttccggg agggggatgc acgccggttg ccgtatcgga ccgactccta
     2401 cgacgtcgtc ctcgtcctcg ggaacagctt cggctacttc gactccgtcg aggaagacct
     2461 gcggatcctc accgagctcc ggcgcatact caaaccctgg gggcaggtgc tcctggatgt
     2521 ggccgacggc gaatacctca aagagcactt tcagccccgg tcgtgggagt ggatcgatcg
     2581 cacgatgttc gtctgccgtg aacggtccct ctcgctcgac ggccagcgcc tgatctcacg
     2641 ggaggtgatc accgacgtcg acaagggcgt cgtggccgac cagttctacg ccgaacgcct
     2701 ctacacccgc gatgcgctcc gacaactcct ggaacgggcc gggttctccc gtatcgcctt
     2761 ccacgagatc gcaacggaat cacagcgaaa ccaggacctc ggcatgatgg agcggcgcca
     2821 catcgtcacc gcagcaatca ggaaagagtg ggcgaaggca aaggcgaagg ggcaggagag
     2881 ggcaaaacat gtcgcggtga tgctcggcga cccgaccaag gccgacgccc tgaaaccgtc
     2941 ctgcgtcttt gacgacgacg atttctacac catcgaccag atgaaggcgg cgctccggga
     3001 actcaaaggt taccggttca cctacctctg caaacacgac accatgatcc cggacctcac
     3061 gaaactgaag ggtaaggtgg actacatatt caacctctgc gacgaggggt tcaacaacga
     3121 cccacgaaaa gagctccacg tgccggctct cctggagatg ctcgacatac cctacacggg
     3181 atccgcgccg cagtcgcttg cgttctgcta cgataaatcg ctcgtgcgcg gcatcgcaaa
     3241 ggagatgggg atcccggtgc ccgatgcctg cttcatcacc ccgggcgacc gcacctacga
     3301 tgtcgggatg aagttcccgg tgatcgtcaa gccgaactca ggcgactcct cctacggcat
     3361 cacgcagagg agcatcgccc acaccatcga ggaactctcc gatatcatca gctccctgcg
     3421 gacgacgctc gggtacagcc agtcgctcct tgtcgaggag ttcctcacgg gcaaagacat
     3481 cagcgtcggc atcatcggga acccgtcggg ctactacacg gtgctcccca tcaccgagga
     3541 ggactactcc gccctgcctc cggaactccc ccggatctgc ggctacgagg caaagtggct
     3601 ccccgactcg ccgtactgga agatcgtatc gcggccggca aaccttcccg aggagaccga
     3661 gaaggtggtc gtgcgctgct gccttgccct cttcgagcgc ctggagtgcc gcgactactg
     3721 ccggttcgac tggcgcctgg acgagcacgg caacccgaaa ctcctcgagg tcaacccgaa
     3781 ccccggctgg tgctgggacg gccaccttgc aaagatggcg aagtatgcag ggctctctta
     3841 tcccgatatg ctcgggcggg tcctgaaggc agcagaagag cggttcggca tggaccagga
     3901 gacaggtgcg cgggaggcgg aggcaaagac cgttccgctc gacctctccc ggcaggaggc
     3961 ctgattacct tttccgggag aatgcaggcc actgcatcct cccggtcgtc tttcctatcc
     4021 cacgccgatc tcgctgcagg cgaccggtac ccatcagtat ataatatcct ccggcgcata
     4081 gatcataaat tatggccgat gaatcgtacc ggaccgcaac ggtccagggc agagaggtcc
     4141 cctacgatcc ggagcaactc cgcaagatca gcgagcaccc ctgctactcc gacaaagcat
     4201 gccacgcctt tggacggtgc cacctccccg tcgccccgaa gtgcaacatc cagtgcaact
     4261 actgtgtgcg ggacttcgac tgcgtgaacg agagccggcc gggcgtgacg agcaagattc
     4321 tcacaccgga ggaaggcctc gacctggtca ggaaggtcgt caaggactat ccgtttgtga
     4381 aggtgatcgg catcgcggga ccgggcgaac cgcttgcaaa ccccgagacg ttcgaagcgc
     4441 tcaggctggt tcacgaggag ttcccgcacc tgatcatgtg cctcagcacg aacggcctca
     4501 tgctccccga gtcgatcgat gaactggcga agtacgacgt cgggaacgtg acggtgacgc
     4561 tcagcgccat cgatccggcg atcggagaga agatctactc ctgggtcgag tacaaaggga
     4621 agaagtatcg cgggcgcgag gctgcggagc tcctcctctc ccagcagttg aaggggatcg
     4681 agatggcggt cgcaaagaag atgttcgtca agatcaacac cgtctacata cccgggatca
     4741 acgacgagca tatccccgcg atcgcaaaga aagtcggcga gatgggagcg ttcaccttca
     4801 acgtcatacc gctcatcccg cagtataagt tcgccgacat cacccccccg acggcaaagg
     4861 ataagcggga gatgcaggac cggtgcgccc cgttcatcaa gcagatgcgc cactgcgcac
     4921 gctgccgggc ggatgcgatc gggaagttag gccaggacgt gcagtcctgc gtctaccagc
     4981 agatgaagga tgagaaggca gaataaccaa ctttttttcg gccggccatg aggacgatcg
     5041 aactccggcc cgaccagccg ctcgacctcg acctgacgct ctcctgcggg caggctttcc
     5101 gctgggagaa tgtggacggg tggtggcagg gcgtcgtcga cggtcgggcc gtccggatac
     5161 ggcaggacgc agaccggctc acgtttgagg gggcggatgc ggcgttcgtc cgcgactacc
     5221 tccggctcga ccaggacctt cccgccattc tcgcatcgat cgaccgcgac ccggttatag
     5281 gcgccgccgt ccgggagtgc cgcggcctcc gcctcgtccg gcagcagccg tgggagtgcc
     5341 tggcctccta catctgcgcc acgaacacga acatcccggc ggtgaagcgg cgggtcgccc
     5401 tgatggcaga gcgctacggg agagcgatcg acgggccgtc cggcatgacg tacgcgtttc
     5461 cggagccgga ggcgctcgca gaggcttcac gcacggatat atgggactgc aaacttggtt
     5521 acaggacgga ttatatctgc gaggccgccc gcttcgcaag cgagaacccc ggctgggcgg
     5581 agcgaatcgc ggcccttccc ttcgaggatg cgcggcgggc gctgatggag tttaaaggcg
     5641 tcgggccgaa ggcggcggac tgcgtgctcc tctttgcgtt cgggttcttt gaggcgttcc
     5701 cggtcgatgt ctggatacgc cggatcgtgg gggagaccta cctctccgac cttgcaggcc
     5761 ggagttgcac cccggcggag tacgaacgga tccggcgatt tagccgcgac tacttcgggg
     5821 agtatgccgg gtatgcacag gagtacctct actgcgcgcg gggcccggca cgtcgtcagc
     5881 ggtgaggctc accctttccg gctgcggacc cgctgccggg tgagtccggc tgccgcgagc
     5941 aagacgaaga taacggcgcc gatgagagcg aaggtgccga tacctgcaag accgataggc
     6001 actccgcata ccgcgatcat gggcgatgcg gtggcgatag cgacttcgcc catcttcgag
     6061 gggagcgtga actaccccgc caacaacgag atctcggtgc cggagcatcg tcgtgctgtg
     6121 aactaccccc cggctttcgc agggggcttc ccggccgtcg tcctctcccc attgggagcg
     6181 aacccacagg atcttcggca tgctccacgc ctgcacgtgc aaacggtttg acgtcgaacg
     6241 cggcgttcag gtcccggtcg tgatgcatcc cgtattagga ataggtccac tcccggactt
     6301 tgagggtcag atcggcattc cggtagagcc ggcactggta cctccgcaac atacgataat
     6361 atcatggtag ccatcccccc ggcatgaact gttgcggcgg ttgaaacggg tcgagtatgt
     6421 tgaggtggga tgtgcggggc aggagaaagc gttctggtac ctcaacctga cggataagat
     6481 gagggatcaa ctgaatgcga tggggtttca ggatctcttc cccgagaaga gattgagtca
     6541 gtgtaagtta taaatccgga aagtcaggtt tgtgaaggtg atatcagtaa ttgcctggcg
     6601 cactaccgat cgtttccact ctgatctccg tttgatgttc gtaacaacat ttttcatcat
     6661 tcacgtttca ttctctctaa ctcgggggat tacatcatga tagtacgatt tgaaactacc
     6721 tatgaagacg gatattggtc tgccagcgca atcggataca gtatctttac cgatggtgaa
     6781 acattcgaaa aattgcttaa aaacattgaa gaagctgtac tgctgtattt cgaagacgaa
     6841 gtggaaccca acgaacaaat cacgatccag acccagatta cacaccaggt gcatcacgtt
     6901 gccacatctt ccagttgtta gcagcacaga tatcatcagg gttctcaaaa agcttggata
     6961 ttcttttgtc caccagcgcg gaagtcacag tgaacaatat cttaaacgat atcgcaaacc
     7021 ataacaatat gactaaacaa gaactcattg atttgttctt gcg
//
LOCUS       DAOSII010000097         7049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_9706_length_7049_cov_58.195310, whole genome shotgun sequence.
ACCESSION   DAOSII010000097 DAOSII010000000
VERSION     DAOSII010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7049
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9706_length_7049_cov_58.195310"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="PLV90_07745"
     CDS             complement(<1..565)
                     /locus_tag="PLV90_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="METTL5 family protein"
                     /protein_id="HOF97044.1"
                     /translation="MNLRHLEMRLERLEGFERPIARLEQYQTPAPVAARLLHHAAMQG
                     AIEGRRVCDIGCGTGILACGAALLGASAVAGVDIDPAAIDVAHRNARALGVSVEFLVA
                     DIRDPTVDRTRLACDTVVMNPPFGAQKAHADRPFIDRALDLADVVYGIFNEGSTPFVA
                     AYTEGRAAIEEVIRCAFPLRRTFAHHRK"
     gene            complement(562..1515)
                     /gene="dph2"
                     /locus_tag="PLV90_07750"
     CDS             complement(562..1515)
                     /gene="dph2"
                     /locus_tag="PLV90_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043364 - glycyl-radical enzyme activating
                     activity [Evidence IEA];
                     GO_process: GO:0017183 - peptidyl-diphthamide biosynthetic
                     process from peptidyl-histidine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthamide biosynthesis enzyme Dph2"
                     /protein_id="HOF97045.1"
                     /translation="MSLIPVSDLIAKLHERGARSVALQFPAGLARQAPQVAAALRDAG
                     FFVLVSGDPCYGACDLALDALRHADVLVHFGHAPVEDRPGVIYEPVRFDFDVAVLDRA
                     LPLLSTRRIGLVTTVQHIHLVDAMAAYLREHGIEAAVAPGDGRTPFPGQVLGCNFAAA
                     RATGADEILFVGTGVFHPVGIRLATKKRVVALDPYTGEAGEVDADRLVRRRAAVMERA
                     RGAANFGIIVSTKSGQQRMDLARRLAALSDRAVLVMMREVSPGELLDLGFAAYVNTAC
                     PRLAYDDQARFPVPVLAPPEFEILCGARAWDDYTIDEYLSP"
     gene            complement(1493..2044)
                     /gene="hpt"
                     /locus_tag="PLV90_07755"
     CDS             complement(1493..2044)
                     /gene="hpt"
                     /locus_tag="PLV90_07755"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine/guanine phosphoribosyltransferase"
                     /protein_id="HOF97046.1"
                     /translation="MLERLIQSLEASPVMRRGEYNYFIHPITDGVPLLEPAILREIGC
                     AMVRILDLAGVDKIVVCEAMGIPIGVALSMMTDIPLVIVRKRSYNLPGEVAVHQATGY
                     SKGELYLNGVGAGDRVVVIDDVCSTGGTLAALVSALEQVGADIADICVVIGRGDVDLG
                     RPLKTLVRIEVSEDRVHVVDTCL"
     gene            complement(2037..3134)
                     /gene="mfnA"
                     /locus_tag="PLV90_07760"
     CDS             complement(2037..3134)
                     /gene="mfnA"
                     /locus_tag="PLV90_07760"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase MfnA"
                     /protein_id="HOF97047.1"
                     /translation="MREQGCPEGDLFSFLSLQRKKDLGYRNILSSMCTPPHPVAARAH
                     AMFLETNLGDPGLFPGTAELEELLVRRLATLMHHPKAGGYATSGGTESNIQAFRIAKK
                     QKPVESPNVVVPASSHFSFLKACDILGLAMRTAPLDTHFRVDAEAVESLVDKDTVCIV
                     GIAGTTEYGMVDPIAHLADVALDRDVFLHVDAAFGGMVVPFLDRPIPFDFHLPGVDSL
                     SVDPHKMGMSTIPAGCLLVRNPEQFSCLNVDTPYLTVKRECTLAGTRPGASVAAAFAV
                     LEYLGMDGMRAVVAGCMENTRRLVEGMETLGCERAVTPDVNVATFSCSRAPAGWRVST
                     TRAGHMRVVCMPHVTRDVIEAFLKDMSDMHA"
     gene            complement(3127..5415)
                     /gene="ppsA"
                     /locus_tag="PLV90_07765"
     CDS             complement(3127..5415)
                     /gene="ppsA"
                     /locus_tag="PLV90_07765"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HOF97048.1"
                     /translation="MKEMPNILWLEEIKKEDIPSVGGKGASLGEMTAIGLPVPKAFVV
                     TAQAFRKFLVETGIEETLFRRLARLDVDDNGALESVSRDVQDLVLSVDIPDHFREEIT
                     DAYARMGANGMVVAVRSSATAEDLPEASFAGQQETFLNILGDEDLINAVHRCWASLYG
                     ARAIYYRAKQGFDDRSVNIAVVVQELIRSEKSGVMFTSHPVTGEPLTIIEGSWGLGEA
                     VVSGSVSPDNYVFDLRTERVVDRLIAEKEIMIVPEGEHGTTIVNLSGRERTAPVLSDA
                     EVARLATLGKIAESHYGVPQDVEWAIVGDDVFILQSRPITTIRRPEIPRPGTAPAPRD
                     KPGVVLLEGQGASPGVASGRVVIVRDVKDTSAVKEGDILVTKMTNPDMVPAMRRVSAI
                     VTDEGGMTCHAAIVSRELGTPAVVGTKKATKVLQDGQIITVDGEKGTIYEGATAAAAP
                     APAVASAAAAAPAPLITGTLVKVNVSLPEAAKRAAATGADGVGLLRIEHLILGLNKTP
                     GWYVEHNREEEFIGELYGGIKTVLDAFPGKPVWVRTLDAPTDEFRNMKGGEEEPIEHN
                     PMLGWRGIRRDLRSPDQFRLQVEAFKRLWAAGYSNLGVMFPMVNHPGEFIQARAMMKD
                     WGVDVENATLGVMIEIPSSAILVEDFIKAGIRFASFGTNDLVQYTLAIDRNNEHVADM
                     YEPEHPAVLWLIDYAIKACREHGIECSICGQAGSDPAMVAWLVGHGITSVSANIDAVP
                     RIREAVARKERQILLDAARRNA"
     gene            5511..>7049
                     /gene="serA"
                     /locus_tag="PLV90_07770"
     CDS             5511..>7049
                     /gene="serA"
                     /locus_tag="PLV90_07770"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOF97049.1"
                     /translation="MRYRVLVSDPLAEEGIDILKGFCDVDINTGLTEDELVAAIGGYD
                     ALLVRSGTEVTARVIDAGARLKFIGRAGAGVDNIDTEAATRRGIIVANAPEGNTLAAT
                     EHTMAMMLSLARNIPQANASLKKGEWKRSKFIGIELNDKVLGIVGLGRIGNEVAKRAR
                     AMEMKCIAYDPFISKERAAKIGVELVSLDDLFRRADVITVHTPLIKETRHIVNAKTIA
                     TMKDGVRLINCARGGIIDEKALADAIASGKVAGAAVDVFESEPPTDSPLIGLDHVIVT
                     PHLGASTVEAQMNVAISVANQCISVLEGGPAKYVVNAPMVPAEQQALVEPYAVLAQKM
                     GRLLIQLIEGRLESIEITYGGEVAQMPNTKFITRVILKGMLDPILQTPVNIVNAEFVA
                     KERGIRMSETTTEEAQGFRNIICITAKTDRMTETVSGSVSGPNRARIVSIGGYMTDLT
                     PTGHVVISRHTDKPGVIGKAATILGRANVNIAGMQVGRHRPGEEALMVLTVDSSVPAD
                     AMDEI"
BASE COUNT         1250 a         2247 c         2362 g         1190 t
ORIGIN      
        1 ctttgcggtg gtgggcaaac gtccgccgga ggggaaacgc gcaccggatc acctcctcga
       61 tcgccgcccg cccttcggtg taggccgcca cgaacggtgt cgagccctcg ttgaagatgc
      121 cgtagaccac atccgcaagg tcgagcgccc ggtcgatgaa cggccggtcg gcatgcgcct
      181 tctgcgcccc gaacggcgga ttcataacga cggtgtcgca cgcaagacgt gtccggtcga
      241 cggtgggatc gcggatatcg gcgacgagaa actccacgga gacgccgagc gctcgcgcgt
      301 tccgatgggc gacgtcgatc gcggccggat cgatatccac ccctgccacg gccgaggcgc
      361 cgaggagcgc cgccccgcag gcgaggatcc ccgtcccgca cccgatgtcg cagacccggc
      421 gcccctcgat agcgccctgc atggcggcgt ggtgaaggag gcgggccgcc accggcgccg
      481 gggtctggta ctgctcgagg cgggcgatcg gccgctcgaa gccttcgagg cgctcaaggc
      541 gcatctcaag gtgccggagg ttcatgggga gaggtactcg tcgatggtat aatcgtccca
      601 ggcccgggcc ccgcagagga tctcgaactc cggcggcgcc agcaccggga cggggaacct
      661 ggcctggtcg tcgtaggcga gccgcgggca ggcggtattc acgtaggccg caaacccaag
      721 atcgaggagc tcgccgggcg atacctcgcg catcatgacg agcaccgccc ggtcggagag
      781 ggcggcaagc cttcgcgcga gatccatcct ctgctgtccg ctcttcgtgc tgacgatgat
      841 accgaagttc gcggcgccgc gggctctctc catcaccgcg gcgcggcggc ggaccaggcg
      901 gtcggcatca acctcccctg cttcgcccgt gtatgggtcg agcgccacca cccgcttttt
      961 ggtcgcgagc cggatgccga cgggatgaaa gacccccgtc ccgacaaaga ggatctcgtc
     1021 cgcccccgtc gcccgtgcgg cggcaaagtt gcacccgagc acctgcccgg gaaacggcgt
     1081 gcggccatcg ccgggtgcga cggccgcctc gatgccgtgc tcgcggaggt aggccgccat
     1141 cgcgtcgacg aggtggatgt gctggacggt cgtgacgagg ccgatccggc gggtggagag
     1201 gagggggagg gcgcggtcga gcacggccac gtcgaagtcg aaccggacgg gttcgtatat
     1261 gacgcccggg cggtcttcga ccggcgcgtg gccgaaatgg accagaacgt cggcgtgccg
     1321 gagggcgtcg agcgcaaggt cgcaggcacc gtaacagggg tcgccgctga cgagcacgaa
     1381 gaaccctgcg tcgcggaggg cggcggccac ctgcggcgcc tgccgcgcaa gcccggcggg
     1441 gaactggagg gcgacggacc gcgccccgcg ttcgtggagt tttgcgatga ggtcagagac
     1501 aggtatcaac gacatgcacc cggtcctcgg agacctcgat cctgaccagg gtcttgagcg
     1561 gccggccgag atccacgtcg ccccggccga tgaccacgca tatgtcggcg atatcggcgc
     1621 ccacctgttc gagcgcgctg acgagggcgg caagcgtccc cccggtgctg cagacgtcgt
     1681 cgatgaccac gacccggtcg cccgcgccga ccccgttcag gtagagctcc cccttcgagt
     1741 agcccgtcgc ctggtggacc gcaacctccc cgggaaggtt gtaggagcgt tttcggacga
     1801 tcacgagcgg tatatcggtc atcatcgaga gggcgacgcc gatggggatc cccatcgcct
     1861 cgcagacgac gatcttatcg acccctgcaa ggtcgaggat ccgcaccatg gcgcacccga
     1921 tctcgcggag gatggccggc tcaaggagcg gcaccccgtc ggtgatcggg tgaatgaagt
     1981 aattgtactc cccgcgcctc atgaccgggg aggcctccag cgattgtatc aggcgttcaa
     2041 gcatgcatat cactcatatc tttaagaaac gcttctatca cgtcgcgggt gacgtgcggc
     2101 atgcagacga cccgcatgtg cccggcccgg gtcgtcgaga cccgccagcc ggcaggcgca
     2161 cggctgcagg agaacgtcgc cacgttgacg tcgggggtca ccgcccgctc gcagccgagc
     2221 gtctccatcc cctcgaccag tctccgggtg ttctccatgc accccgcgac caccgcccgc
     2281 atgccgtcca tccccaggta ctcgaggacc gcgaacgcgg cggccaccga agccccgggc
     2341 cgggtgccgg caagggtgca ctcccgcttc accgtcaggt agggcgtatc gacgttgagg
     2401 cacgagaact gctccgggtt ccggacgagg aggcatcctg ccggtatggt gctcatcccc
     2461 atcttgtggg ggtcgacgga gagggagtcg acaccgggga gatggaagtc gaaggggatc
     2521 ggccggtcga ggaacggcac caccatgccg ccgaacgcgg cgtcgacgtg gagaaagacg
     2581 tcccggtcaa gagccacgtc ggcgaggtgg gcgatggggt cgaccatacc gtactccgtc
     2641 gtcccggcta taccgacgat gcagaccgta tccttatcca cgagggactc gaccgcctcc
     2701 gcatccaccc ggaaatgggt gtcgagcggc gccgtccgca ttgcaagccc gagtatatcg
     2761 caggccttca aaaacgaaaa atggctcgat gccgggacca cgacgtttgg agactccacc
     2821 ggcttctgct tctttgctat ccggaacgcc tgtatgttcg actccgttcc gccgctcgtc
     2881 gcatatcccc cggctttcgg atggtgcatc agggtggcga gcctccggac gaggagttcc
     2941 tcgagttctg cagtccccgg gaagaggccg gggtcgccga ggttcgtctc gaggaacatg
     3001 gcatgcgccc gggccgcgac cgggtggggc ggcgtgcaca tcgaactcag tatattccgg
     3061 tagccgagat ccttctttcg ttgtaatgaa aggaaggaga agaggtcccc ttcggggcat
     3121 ccctgctcac gcatttcttc tcgccgcatc caacaggatc tgtcgctctt tacgcgcaac
     3181 ggcctcacgg atgcggggaa cggcatcgat gtttgccgag acgctcgtga tgccgtgccc
     3241 gacgagccat gcgaccatag cggggtcgga gccggcctgg ccgcatatgg agcactcgat
     3301 gccgtgctcg cggcaggcct tgatcgcgta atcgatcagc cagagcacgg cagggtgctc
     3361 gggctcgtac atatcggcga cgtgctcgtt gttccggtcg atggcgagcg tgtactggac
     3421 gaggtcgttc gtcccgaacg aggcaaaccg gatgccggcc ttgatgaagt cctcgaccag
     3481 tatggcgctg ctcgggatct cgatcatgac gccgagggtt gcgttctcca cgtcgacccc
     3541 ccagtctttc atcatcgccc gggcctggat gaactcgccg gggtggttga ccatcgggaa
     3601 catcacgccg aggttgctgt agcctgcggc ccagagcctc ttgaacgcct cgacctgcag
     3661 gcggaactgg tcggggctcc ggagatcccg gcggataccg cgccacccga gcatcgggtt
     3721 gtgctcgatg ggttcttctt ccccgccttt catgttccgg aactcgtcgg tcggagcgtc
     3781 gagcgtccga acccagacgg gttttccggg gaacgcatcg agcaccgtct tgatgccgcc
     3841 gtagagttcg ccgatgaact cttcctccct gttgtgctcg acataccagc ccggtgtctt
     3901 gttcagcccg agtatgaggt gctcgatgcg gaggaggccg accccgtcgg cgccggtggc
     3961 ggccgcccgt tttgcagcct cggggaggga gacgttcacc ttgacgagcg tgccggtgat
     4021 gagcggggcg ggcgccgccg ctgccgccga cgcgactgcc ggcgccggtg ccgctgctgc
     4081 cgtcgctccc tcgtagatgg tgcccttctc gccgtcgacg gttatgatct gtccgtcctg
     4141 gagcaccttc gttgctttct tggtcccgac gaccgcgggc gtcccgagtt cgcggctcac
     4201 gatggccgcg tggcaggtca tgccgccctc gtcggtgacg atggcgctga cccgccgcat
     4261 cgccggcacc atgtcggggt tggtcatctt cgtgaccagg atgtcgcctt ccttgacggc
     4321 gctcgtgtcc ttgacgtcgc gcacgataac gacccggccg ctcgcgacgc cgggggatgc
     4381 gccctggccc tcgaggagga ccacgccggg cttatcccgg ggtgccggtg cggtccccgg
     4441 gcgggggatc tccggccgcc ggatcgtcgt gatgggacgt gactggagga tgaagacgtc
     4501 gtcgccgacg atcgcccact cgacgtcctg cgggacgccg tagtggctct cggcgatctt
     4561 gccgagcgtc gcgaggcgtg ccacctcggc gtccgagagg accggagcgg tgcgctctct
     4621 cccggagagg ttgacgatcg ttgtcccgtg ctccccttcc ggcacgatca tgatctcctt
     4681 ctcggcgatc aggcggtcga ccaccctttc tgtgcgcagg tcaaagacat agttgtcggg
     4741 cgagacgctg cccgagacga cggcttctcc aagcccccag gaaccctcga tgatcgtcag
     4801 gggttcgccg gtgacagggt gggaggtgaa catgacgccg gacttctccg accggatgag
     4861 ctcctgcacg acgacggcga tgttcacgct ccggtcgtcg aacccctgct tggcccggta
     4921 gtagatggca cgcgcgccat acagggaggc ccagcatcgg tggactgcgt tgatgaggtc
     4981 ttcgtcgcca agaatattga gaaaagtctc ctgctggccg gcaaaactgg cttccggcag
     5041 gtcttcggcg gtggcgctcg aacggacggc gacaaccatt ccgttcgccc ccatccgggc
     5101 gtatgcgtcg gtgatctcct cgcggaagtg gtcggggata tcgacgctca ggacgagatc
     5161 ctgcacgtca cgggagacgg actccagtgc accgttgtcg tcgacgtcga ggcgcgccag
     5221 acgccggaag agcgtctcct cgatcccggt ctcgacaagg aacttgcgga acgcctgcgc
     5281 ggtcaccaca aacgccttcg gcacggggag gccgattgcc gtcatctcac caagagaggc
     5341 tccctttccc cctacggagg ggatatcttc cttttttatt tcttcgagcc acagaatgtt
     5401 gggcatttcc ttcattctcg caccacacat atacttctca ccattacata aaagatttcg
     5461 gaagaaaaag catcaataca catgatgtcg aatcagatat gggttgaact gtgagatata
     5521 gagtgctggt cagcgacccg ctggcagagg aagggattga tatcctcaag gggttctgcg
     5581 acgtggatat caataccggg ttgaccgagg atgagctcgt tgccgctatc gggggctacg
     5641 acgcccttct ggtgcgttcg ggcaccgagg tgacggcgcg ggtcatcgat gcgggagcga
     5701 gactgaagtt catcggtcgt gccggcgccg gggtcgacaa tatcgatacc gaagcggcga
     5761 cacgccgggg catcatcgtc gcgaacgctc cggaaggcaa taccctggca gccacggagc
     5821 atacgatggc gatgatgctc tcgcttgcgc gcaacatccc ccaggcaaac gcatccttga
     5881 agaagggcga atggaaacgc tcgaagttca tagggatcga gttgaacgac aaggtcctcg
     5941 gcatcgtggg gctcgggcgg ataggcaacg aagtggcgaa gcgggcacgg gcgatggaga
     6001 tgaagtgcat cgcctacgac cccttcatca gcaaggagag ggcggcaaag atcggcgtcg
     6061 agctggtctc gctcgacgac ctcttccggc gggctgacgt cataacggtc catacgccgc
     6121 tgatcaagga gacgcgccac atcgtcaacg caaaaaccat cgccacgatg aaggacgggg
     6181 tgcggttgat caactgcgcc cgcggcggga tcatcgacga gaaggcgctc gcggatgcga
     6241 tcgcgagcgg caaggttgcc ggcgccgcgg tggatgtctt tgagtcggaa ccgccgacgg
     6301 actctccgct catcggcctc gaccacgtga tcgtgacgcc gcacctcggg gcgagcacgg
     6361 tcgaggcgca gatgaacgtc gccatatcgg tagcaaacca gtgcatcagc gtgcttgagg
     6421 gcggcccggc gaagtacgtg gtgaacgcgc cgatggtccc ggcggagcag caggcgctgg
     6481 tcgagcccta tgcggtgctc gcgcagaaga tgggccgcct cctcatccag ctcatcgagg
     6541 ggcgccttga gtcgatcgag atcacctacg gcggcgaggt cgcccagatg ccgaacacga
     6601 agttcatcac ccgcgtcata ctgaagggga tgctcgaccc gatcctgcag acgccggtca
     6661 acatcgtgaa cgcggagttc gtcgcaaagg agcgcggcat ccggatgagc gagaccacca
     6721 ccgaggaggc gcaggggttc aggaacatca tatgcatcac ggcaaagacc gaccgcatga
     6781 ccgagacggt gagcgggagc gtctctggcc cgaaccgcgc gcggatcgtg agcatcgggg
     6841 gctacatgac ggacctgacc ccgaccggcc acgtggtgat ctcccgccac accgacaaac
     6901 cgggcgtcat cgggaaggcc gcgacgatcc tcggccgggc gaacgtgaac atcgcaggga
     6961 tgcaggtggg acgccaccgg cccggcgagg aggcgctgat ggtcctcacg gtcgactcct
     7021 cggtgccggc cgatgcgatg gacgagatc
//
LOCUS       DAOSII010000098         7017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_9758_length_7017_cov_93.869147, whole genome shotgun sequence.
ACCESSION   DAOSII010000098 DAOSII010000000
VERSION     DAOSII010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 7017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7017
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9758_length_7017_cov_93.869147"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PLV90_07775"
     CDS             <1..555
                     /locus_tag="PLV90_07775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HOF97050.1"
                     /translation="ITKEQVAAYRAQVAKGGTDIIERVVKDLGDGRASVTTRRTFKPW
                     LADMATRDQNEILRIISDGQRDGMHPHQIARELRGYFDGTAHNAVTAARTEAQKIRTD
                     ARVATYLKTGVRYLEYIAVGDDSTRPEHLARDGKIYPIDKAPWLGEPNCRCTLIDADY
                     KVEEEGAAVEESDSITLTPEELEA"
     gene            552..884
                     /locus_tag="PLV90_07780"
     CDS             552..884
                     /locus_tag="PLV90_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97051.1"
                     /translation="MTRPPALTDRQIQIIRENLDLFPADILKLPEFTDADVTRHTIRN
                     YQRRLKNAAVIDEEEVLLTRLKQYMNRYGLESRFHGPRGVTGFIRHLETKIHLRAIER
                     DNSENSAV"
     gene            949..1953
                     /locus_tag="PLV90_07785"
     CDS             949..1953
                     /locus_tag="PLV90_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97052.1"
                     /translation="MTRGDIPHTNAFANLHDVTLQRLDVYHRNNGRYVFYDSKHFAPT
                     VDHWNTVPVIYVETEPGKPAEHPRFEDVVANTLPAKFRTVGSVSAAHLTETGEKVLKG
                     AIVFSDPAIAAKANAGELSLSTGLASPEAPDPRLPGATRIAGPVTPNHILVFDRGACP
                     NCYPNDSGAMFHNLQEPDMPDDETKGLLKTIADALTRREPAPVQHVNLTEFENLKKEL
                     ETAKAQTAELANLKQELETLKAEKATAEKDAKWNAMKANLPEGWLGAKEPETRKEFEA
                     DPGAFALKVVAFKNTQPQEQQAEGAGVAGGSGDAESTEEQKFANMAAEVAKATGIQFV
                     "
     gene            1966..2349
                     /locus_tag="PLV90_07790"
     CDS             1966..2349
                     /locus_tag="PLV90_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97053.1"
                     /translation="MAYEAGEFFPGARVQRVTASADIAKGAVVTITPAAASAAATCAE
                     DGVGPFAVAIEAVANGKTGRVVTKGEVAVDCSGNCYTGAVVTGSGGKVKVCDTDPSGN
                     WIKPLGRMTVGGANGTVGVVDLGGF"
     gene            2351..3226
                     /locus_tag="PLV90_07795"
     CDS             2351..3226
                     /locus_tag="PLV90_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97054.1"
                     /translation="MGNTGILGKVQIEGNWTQKRLILPVIQAALERTQLASPAIGPTM
                     TYAKLKGTIPLLGPVPVQSQLDEFEHAVGGGGKPSGFDIEVLKDRVVLYVSDEAEIES
                     DVGNPMSLQQQAAAGALAANLNKLIAERLNTTPQVYGTGGDLGNWTSAKPTLAVGKMA
                     AAMGVHQPTALVMGTLAGAYYVDAVGDKVAIANLAEWRGATSIHPTLNIPVFISTDID
                     KLDDTSGNRFVFGVCNATPGVVTVISKIKARQYDDPKLGAQVYQYDIWRSPFSNIQQT
                     SGKNLGVMRGLMTES"
     gene            3249..3854
                     /locus_tag="PLV90_07800"
     CDS             3249..3854
                     /locus_tag="PLV90_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97055.1"
                     /translation="MAFAPCNRYGVAGIIDDEGRHIVGSDVLHAVRQGRAFRFAHRFT
                     AVAPAAAVDILLDPSANDAATVVRLVLEIDTGVDCSVGIYEVPTVIAPGTELIAYNLN
                     RNGDPSMNSVPAVYHTPTVSPAGTLAAPVAYVTAALPVDILYQGTMGDVGAGGAAHGT
                     LPEIYLDRTKKYLVRVTNIGTGAGNIVVAGRLIREPNYYEG"
     gene            3998..4411
                     /locus_tag="PLV90_07805"
     CDS             3998..4411
                     /locus_tag="PLV90_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97056.1"
                     /translation="MFFQKTIKNTILEKFLDQAWQGFLKYRCAGNKAAYREAKKNPDA
                     VFQYDDQLLALLNRVMKESIAEHHTDNDAARKQQLMRQATDITLTLLNEDIYYRARCK
                     EHLRDILAAVAEHPEYLDLSPEEEQNIRRWNGCSP"
     gene            4520..4945
                     /locus_tag="PLV90_07810"
     CDS             4520..4945
                     /locus_tag="PLV90_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97057.1"
                     /translation="MPTSGNVLALLPVLTPYTPTSAQFDLLYPYALDEFKGDDPGCSE
                     AGAERALAYLVAHYLASKDGDVGLQSEKIDDYGYTLSATAARSSRWYAEYQRQIDRCR
                     EALAIGPAALAGVQHADVSGLSDLHLDQNPVVRVRRDGL"
     gene            4942..5181
                     /locus_tag="PLV90_07815"
     CDS             4942..5181
                     /locus_tag="PLV90_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97058.1"
                     /translation="MSLPNDASLGALSDREILLMVYAKVDTIAEASNDHEDRLRVLEA
                     QSNRTLGLMAATGGGSGAVAGGVVAVVMKLLGGFG"
     gene            5178..5477
                     /locus_tag="PLV90_07820"
     CDS             5178..5477
                     /locus_tag="PLV90_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97059.1"
                     /translation="MSLAGLLNQEVQIKARTGTTYTGDPEYATAATYPARISYKPRRV
                     FVNGVERTSTARITVAVPVFDEDLIVLPDGTERGSLQVKRTHGGGGTFHHSTIDI"
     gene            5499..>7017
                     /locus_tag="PLV90_07825"
     CDS             5499..>7017
                     /locus_tag="PLV90_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97060.1"
                     /translation="MANRVSGEIHKKGGILQQTFGETVANAYGLVFYQAADGKLYRAQ
                     ANAETTVAGVLYLCADAATVKDTVGNTLAQGRAEKTGWSWTVGKLVYVSPTVAGGLTQ
                     TVPTGTQKIRPVGFATKTDQIDFRPLWGTGASYTVNIDGIAATKGDILVRSANAWGAV
                     PGTTIGAHPLVPDLRNRILRIKDGSGNDLEIHQVWIPPFDSEGFSDPSLNGIECGGFW
                     IDKYQACMHDASNVSRGSSGTNSPGTHGAASKPGVVIWTDINWNNSKIAIENRGGAEN
                     KKTGTCAAFGAGSPSKFYVAAITDLIGRRVRITQGSVTYVRRIVKTGGDTGADENAAK
                     LVEIYPALPANITAADTYEIVQHFLPGQYEWFSLAAWAMKYRYQYGLGYPKGNTDWGK
                     DAADPRNAIYEGRPDPVAPGYGGNAISRCLAGTGPTSWSLNGKESGVYDLVGNAWEWV
                     DMLIGTADHTIDAEYPGAGLVLPTANGNIATLYAPTKDGNRSLAADAFSPATVGSA"
BASE COUNT         1484 a         2290 c         2187 g         1056 t
ORIGIN      
        1 atcacgaagg agcaggtcgc cgcctaccgg gcgcaggtcg cgaagggcgg gaccgatatc
       61 atcgagcgcg tcgtcaaaga cctcggcgac ggtcgcgcct ccgtcaccac ccgccgcacg
      121 ttcaagccgt ggctcgctga catggccacg cgcgaccaga atgagatcct ccgcatcatc
      181 agcgacgggc agcgcgacgg catgcaccca caccagatcg cccgggaact ccggggctac
      241 ttcgacggca ccgctcacaa cgccgtcacc gcagcacgga cggaggcgca gaagatccgg
      301 accgacgccc gggtcgcgac ctacctgaag accggcgtcc gttacctcga gtacatcgcc
      361 gtcggcgacg actcgacgcg accggaacac ctcgcccggg acggcaagat ctacccgatc
      421 gataaggccc cctggctcgg cgaaccgaac tgccggtgca ccctcatcga cgcggactac
      481 aaggtcgagg aagaaggtgc cgccgtggaa gaatccgaca gcatcaccct aacaccagag
      541 gaactcgaag catgacccgc ccgccagcac tcaccgatcg ccagatccag atcatccggg
      601 agaacctcga cctgttcccg gccgacatcc tgaaacttcc cgagttcacc gacgccgatg
      661 tcacccggca cacgatccgg aactaccagc gccgcctcaa gaacgccgcc gttatcgacg
      721 aagaagaggt cctcctcaca cgcctgaaac aatatatgaa ccggtatggc ctcgaatcac
      781 ggttccacgg cccgcgagga gttaccgggt tcatccggca cctggaaacc aagatccatt
      841 tacgcgcgat tgaacgcgac aattccgaaa acagcgccgt ataatgcgta gagtgcccaa
      901 aaaagggcca caatttttta tatattggat gcctgccaag catattgtat gactcgtgga
      961 gatatccccc acacgaacgc ttttgccaac ctccacgacg tcacgctcca acgcctcgac
     1021 gtgtaccacc ggaacaacgg gagatacgtg ttctacgact cgaagcactt cgcgccgacc
     1081 gtagaccact ggaacaccgt cccagtgatt tatgttgaga cggagccggg gaagcctgca
     1141 gaacatcccc ggttcgagga cgtggttgcc aacacccttc ccgcgaagtt ccgcacggtc
     1201 ggcagcgtat ccgccgccca cctgacggag accggcgaga aagtgctcaa aggggccatc
     1261 gtgttctcgg acccggcgat tgcggcaaag gccaacgcgg gcgaactctc gctctcgacg
     1321 ggccttgcct ccccggaggc cccggatcct cggctgcctg gagcaaccag gatcgccggt
     1381 ccggtgaccc cgaaccacat cctcgtgttc gatcgcggag cgtgcccgaa ctgctatccg
     1441 aacgacagtg gagcaatgtt ccacaatcta caggaaccag acatgcctga cgacgaaacc
     1501 aagggactgc tcaagacgat tgccgacgcg ctcacccggc gcgagccggc acccgtacag
     1561 cacgtcaacc tgaccgagtt cgagaacctg aagaaggaac tcgaaacagc gaaagcacag
     1621 accgcagaac tcgcgaacct caagcaggaa cttgagaccc tcaaggccga gaaggcaacc
     1681 gccgagaagg acgccaagtg gaacgccatg aaggcaaacc ttccggaggg ctggctcggt
     1741 gcgaaggagc ccgagactcg gaaggagttt gaggctgacc ccggcgcgtt cgcgctcaag
     1801 gtggtcgcgt tcaagaacac gcagccgcag gagcagcagg ccgagggcgc tggcgtggcc
     1861 ggggggtccg gagacgcgga gagcaccgag gaacagaagt ttgcaaacat ggccgcagag
     1921 gtcgcgaagg cgaccgggat ccagtttgtg tgaggtgaga aagacatggc atacgaagca
     1981 ggagagttct tccccggcgc ccgggtgcag agagtcacag caagcgcaga tattgcaaag
     2041 ggcgccgttg tcacgatcac cccggcagca gcgtcggccg cagcgacctg tgctgaagac
     2101 ggtgtcgggc cgttcgcggt cgcgatcgag gccgtggcga acggcaagac cggccgcgtc
     2161 gtcacgaagg gcgaagtcgc agttgactgt tccggcaact gttacaccgg cgctgttgtg
     2221 actggaagtg gcgggaaagt caaggtatgc gacaccgacc cgtccggcaa ctggatcaag
     2281 ccgctcggcc ggatgactgt cggcggcgca aacggcaccg taggtgtcgt cgacctcgga
     2341 gggttctgat atgggaaaca caggaatact cggtaaggtc cagatcgaag ggaactggac
     2401 gcagaaacgc ctgatcctcc cggtgatcca ggcagcgctc gaacgcacac agcttgcgtc
     2461 gcccgctatc gggccgacga tgacctacgc gaaactcaag ggcacgatcc ccctcctcgg
     2521 ccctgtcccg gtccagtcgc agctcgacga gttcgagcac gcggtcggcg gtggcggtaa
     2581 accgtccggg ttcgacatcg aggtgctcaa ggaccgcgtg gtcctgtacg tttcggatga
     2641 ggcggagatc gagagtgatg tcgggaaccc catgagcctg cagcagcagg ctgcagccgg
     2701 cgccctggcg gcgaacctga acaaactcat cgccgagaga ctcaacacga ccccgcaggt
     2761 ctacgggacc ggcggcgatc tcggtaactg gacgtcggcc aagcccacgc tcgccgtcgg
     2821 caagatggct gcagcgatgg gcgttcatca gccgaccgcg ctcgtgatgg ggacgctcgc
     2881 gggtgcgtat tacgtcgatg cggtcgggga caaggtcgcg atcgccaacc tcgccgaatg
     2941 gcgcggtgcc acgtccatcc acccgacgct caacatcccg gtcttcatca gcactgacat
     3001 cgacaaactc gatgacacga gcggcaaccg gttcgtcttc ggtgtctgca acgcgacgcc
     3061 cggcgtcgtg accgtgatct cgaagatcaa ggcgcggcag tacgacgacc cgaagctcgg
     3121 cgctcaggtc taccagtacg acatctggcg ttcgccgttc tcgaacatcc agcagacgtc
     3181 tggcaagaac ctcggcgtga tgcgcggtct catgacggag agctaaccct ctctgttttg
     3241 gaggcactat ggccttcgca ccgtgcaacc gctacggggt tgctggcatc atcgatgacg
     3301 aagggcgaca catcgtcggg agcgacgtgc tccacgccgt gcggcagggg cgggcgttcc
     3361 ggttcgctca ccggttcact gccgtcgccc ctgcagctgc agtcgatatc ctgctcgatc
     3421 cgagcgcgaa cgacgccgcg accgtggtcc ggctcgtcct ggagatcgac accggcgtcg
     3481 actgctccgt cgggatctac gaagtgccga ccgtcatcgc gcccggcacg gaactcatcg
     3541 cctacaacct gaaccggaac ggcgacccgt cgatgaacag tgtgcccgct gtctaccaca
     3601 cgccgacggt ctcgcccgct ggcacgctcg cagccccggt cgcctacgtg accgccgcgc
     3661 tcccggtcga catcctctac cagggtacga tgggcgatgt cggagcaggg ggcgcggcac
     3721 acggcacgct accggagatc tacctcgacc ggacgaagaa gtacctggtt agggtcacga
     3781 acatcggcac cggggcaggg aacatcgtcg tcgccggccg cctgatccgg gaaccgaact
     3841 actacgaggg ctgaccatgt ccgaatcgtt cgcatcccgg tttatgagcc ccgatgagaa
     3901 gcgggcgatg atcgcccgtg accctcaatc agctcaggca ctccacaacg ggccgcggac
     3961 tgggttcgcg cacaattgtt acgccggcgg gctcgggatg ttctttcaga agacgatcaa
     4021 gaacacgatc cttgagaagt tcctcgacca ggcgtggcag gggttcctga agtaccggtg
     4081 cgccgggaac aaggcggcct atcgtgaggc aaagaagaac ccagatgccg tcttccagta
     4141 cgacgaccaa ctcctcgcgc tgctcaaccg tgtcatgaaa gaatcgatcg cggagcacca
     4201 caccgacaac gacgccgccc ggaagcaaca gctcatgcgg caggcgaccg acatcacgct
     4261 caccctcctc aacgaggaca tctactaccg ggcacggtgc aaagagcacc tccgcgatat
     4321 ccttgccgct gtcgccgagc acccggaata cctcgacctc tcgccggagg aggagcagaa
     4381 catccggagg tggaacgggt gcagcccgtg atcgaccctc actaccctgc aatggtgcag
     4441 gcgttcgaga ggtcaaccgg gctcccatat cctgccgggc tcacagtgat caccgaccgg
     4501 ctcttcgagg aggatcccca tgccgacgag cggtaacgtt cttgctctgc tcccggtcct
     4561 gaccccctac acgccgacat cggcacagtt cgacctgctc tacccctacg cgctcgacga
     4621 gttcaagggc gacgatccgg ggtgcagtga ggcgggggcc gagcgggcgc tcgcctactt
     4681 ggtggcacac tatctcgcga gcaaggacgg cgacgtcggg ctccagagtg agaagatcga
     4741 cgactacggc tacacactct ccgccacagc ggcgcgttca tcccgctggt acgccgaata
     4801 ccagcgacag atcgaccggt gccgcgaagc actcgccatc ggcccggcag cgctcgcagg
     4861 cgtccagcac gcggacgtct ccgggctttc tgacctgcac ctcgatcaga accccgtcgt
     4921 gcgcgtgagg agggatggcc tgtgagcctg ccgaacgatg ccagcctcgg tgcgctctct
     4981 gaccgggaga tcctcctcat ggtctacgcc aaggtggaca cgatcgccga ggcctcaaac
     5041 gatcacgaag accggctccg cgttctggaa gcgcagagca accggaccct cggcctgatg
     5101 gcggcgaccg gaggggggtc gggagccgtc gccgggggcg tggtcgcagt agtcatgaag
     5161 ctcctggggg ggttcggatg agcctcgcgg gccttctgaa ccaggaggtg cagatcaagg
     5221 cgcggaccgg caccacctac accggcgatc cggagtacgc gacagccgct acgtatccgg
     5281 cccggatctc ctacaagccg cgccgggtct tcgtgaacgg ggttgagcgg acctcgaccg
     5341 ctcggatcac cgttgcggtc ccggtcttcg acgaggacct gatcgttctg ccggacggca
     5401 ccgagcgggg atcgctccag gtcaagcgca cacacggggg cggcgggacg ttccaccatt
     5461 cgaccatcga catctgaaaa aaggaggaaa caacagcaat ggcaaatcga gtatcaggag
     5521 aaatccacaa gaagggaggc atcctccagc agacgttcgg cgagaccgtc gcgaatgcgt
     5581 acgggcttgt cttctaccag gcggccgacg gtaaactcta ccgggcgcag gcaaacgccg
     5641 aaacgactgt cgccggcgtc ctttacctct gcgcggacgc agccacggtc aaagacaccg
     5701 tcggcaacac gctcgcgcag ggccgggccg agaagaccgg ttggtcatgg accgtgggaa
     5761 aactggtcta cgtctcgccg accgtggccg gcggattgac acagaccgtg ccgaccggga
     5821 cgcagaagat ccggccggtc gggttcgcga ccaagacaga tcagatcgac ttccggcccc
     5881 tgtggggcac cggggcgagc tacaccgtga acatcgacgg tattgccgca acgaagggcg
     5941 acatcctcgt ccggagcgcg aacgcatggg gggccgtgcc cggcaccacg atcggcgcac
     6001 acccgctcgt ccccgacctc cgcaaccgca tcctccgcat caaggacggg tcgggcaacg
     6061 accttgagat tcatcaggtc tggatcccgc cgtttgactc ggaggggttc tcggatccga
     6121 gcctgaacgg gatcgagtgc ggcgggttct ggatcgataa gtatcaggcg tgcatgcacg
     6181 acgcgagcaa cgtctcgcgg ggctccagtg gcacgaacag tcccggcacg cacggtgccg
     6241 cctccaaacc cggggttgtc atctggacgg atatcaattg gaacaactcg aaaattgcga
     6301 tcgagaaccg gggcggcgct gagaataaga agaccggcac ctgtgccgcg ttcggcgccg
     6361 gtagcccatc aaagttctac gtcgccgcga tcaccgacct gatcgggcgc agggtccgga
     6421 tcacgcaggg aagtgtcact tatgtccgcc gtatcgtcaa gaccggcggc gacacgggcg
     6481 cggacgaaaa cgccgcgaaa ctcgtcgaga tctacccggc actcccggca aacatcacgg
     6541 ccgcggacac ctacgagatc gtgcagcatt tcctgcccgg ccagtatgag tggttcagcc
     6601 tggctgcgtg ggcgatgaag taccgctacc agtacggcct cggctacccg aaaggcaaca
     6661 cggactgggg taaggacgcc gccgacccgc ggaacgcgat ctacgagggg cggccggacc
     6721 cggtggcccc gggatatggc ggcaacgcga tctcccggtg tctcgcgggc acggggccga
     6781 cctcatggag cctcaacggc aaggagtcgg gggtctacga cctcgtcggg aacgcctggg
     6841 agtgggtcga catgctcatc gggacagcgg accacacgat cgacgccgag tatccgggcg
     6901 cgggtctcgt gctgccgacc gcaaacggca acatcgcgac actctacgcc ccaacgaagg
     6961 acggcaaccg gtccctcgcg gcagacgcgt tctctccggc aacggttggc tctgcaa
//
LOCUS       DAOSII010000099         6878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_10016_length_6878_cov_47.233475, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000099 DAOSII010000000
VERSION     DAOSII010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6878
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10016_length_6878_cov_47.233475"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PLV90_07830"
     CDS             complement(<1..695)
                     /locus_tag="PLV90_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HOF97061.1"
                     /translation="MSDVFWLSKTGMHDLAHLHFADADASYEDARYAIFGVPYDATTS
                     FKPGTRFGPRAIREASFNFEPYDPSTGVDFSEVPIVDLGDLMVLRLPEDLVGQVADVT
                     GDIVRDGKVPVMLGGEHTATIGAVRAVRPDVYVVCDAHLDLRDELDGTAYSHGCVTRR
                     VLDTVDDVVIIGARSGDAGQFEVAAERTRLYTADAVRERGIAAVLGEVLGEFGGRRVY
                     LSIDADAIDCCLTP"
     gene            complement(688..1065)
                     /locus_tag="PLV90_07835"
     CDS             complement(688..1065)
                     /locus_tag="PLV90_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-5A"
                     /protein_id="HOF97062.1"
                     /translation="MKEQTEVGKLKEGRYVVVDEEPCRIVSIAISKPGKHGAAKSRID
                     CIGIFDGVKRSIVQPVSAKTYVPVVERKMAQVISIAGTTVQLMDVKDFDMFELAVTED
                     QAAGLEPGKEIAYIASLGKKKLE"
     gene            1160..1936
                     /locus_tag="PLV90_07840"
     CDS             1160..1936
                     /locus_tag="PLV90_07840"
                     /EC_number="3.1.3.11"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     fructose-bisphosphatase/inositol-phosphate phosphatase"
                     /protein_id="HOF97063.1"
                     /translation="MEFIRACDDLAGAVSDAVAGLVGTPEAGRCVRMGADGTPTKLID
                     QVAEDIVVDYFTQNPFCRRLISEELGCADMGGDTGTIFLDPVDGTYNAVAGIPFYALS
                     IAYAEGGIVQAGYVKNLATGETFHAVRGHGAYLDGHPIRVSERALLEKSAMSVYGRKF
                     DPTRVLGINRQIRRWRLLGASALELCYVGCGRIDGFVDVRGTLRVTDAAAGMLVCEEA
                     GGTVSDLEGNALVFPDEVSVGRSLVATNGILHNKVIEYLR"
     gene            1943..2755
                     /locus_tag="PLV90_07845"
     CDS             1943..2755
                     /locus_tag="PLV90_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOF97064.1"
                     /translation="MKIVLVSRIDDSDALRYTADLARDLEALGHDVAFEEGTARHLGK
                     EGVPFESIAGDLVVVVGGDGSVLLTVHQMRKQVPVLGINWGEVGFLADLEPDEARAFF
                     SAEKTALRVERRLRVSLSVDGRWLGNALNEAVIVTDRPAKMLRFGVYVDGTPAERFRA
                     DGLLISTPTGSTAYAMSAGGPIVDPQIEGFLLVPLAPYMLSSRPHIISTGRNLEITLE
                     TEKPAHLVIDGQSTFELDREATIRVKKADEPALFVYTGKPFFEKVNHKLRNL"
     gene            2813..3529
                     /locus_tag="PLV90_07850"
     CDS             2813..3529
                     /locus_tag="PLV90_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HOF97065.1"
                     /translation="MEDQMRTKLPYAEIAETLKTTLDLRGSPVAVKLAKSPEGIPEGV
                     KPIEETVRHCQMVSKARLEGAIFYATTDKHVCMGGGWALGLKELTRSLRTGEFYYKLG
                     KFESWAACMRTIRQVPHVPELETYATVYAPLEKTPFDPHVVIIIAEPRTMLKLAQATL
                     YHLGGRIESTMSGIQSVCADATALPYLTGKINYSLGCDGSRRFSGIEDNELVMGIPAE
                     ILPEFARALEIVTAAPGSVR"
     gene            3634..4122
                     /locus_tag="PLV90_07855"
     CDS             3634..4122
                     /locus_tag="PLV90_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HOF97066.1"
                     /translation="MKLEMRDQPGQLVAALKPISAVGGNIIAVIHQRETSTPAEALDV
                     QIVLELPEGRLPNLLELFRAQGINVVRIGEERLLYECTLIMIGHLMHTDLSDTVDRID
                     RTGSAEVTELSLVMPAIDTPSSARITIRSTTRAEMKKAMRILRAVAKQKDLLIIEPLE
                     DA"
     gene            4122..5108
                     /locus_tag="PLV90_07860"
     CDS             4122..5108
                     /locus_tag="PLV90_07860"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOF97067.1"
                     /translation="MRIAILGFGSVGRGIARVLLAKDLGIAVTGLADSKSGIIDAGGI
                     DLAAALARKEGGGPCGNPGVSPADVVAKAEYDVLVEVTPTNVETGEPALGHIREALRR
                     HKHVVTSNKGPIALDYPGLRALAEANGVLLKYEATVCGAIPLIHAMQEGLAGNTISRL
                     YGVFNGTCNYILTRMAAEGLTYEQALAEARELGYAEADPTYDVEGIDAGIKLVILANT
                     ILDLSTTLDDVERTGITLLSPEALQLAEDQDCTIRLIGEIIPDAGVLRVSPRIIPKTH
                     PLVVEGTLNAVTVEADLAGDLTFIGKGAGSTETASAVIGDLLYIRERYVKGS"
     gene            5092..6273
                     /locus_tag="PLV90_07865"
     CDS             5092..6273
                     /locus_tag="PLV90_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOF97068.1"
                     /translation="MSRVLERRKQYLRLMRQVTLERGSFTVADIAEATDTPRSTVQDW
                     VNRLVEEGCVLLTEEQRGRHAAHYAASSVMPESACRRVFTTVDGGEVEIYHECMSGGC
                     AAFCEFHHARAGGALQSVRRDGTLLRERAVLGRRDVAVGLDPAPAVGIVGVFHEGDYI
                     RQHIRCIGGPAYSLTDMMSFAEGVCGVTVRREGAVVEGEVITRALAYVAIGIDDTDTG
                     DEGATFALALALLQHLAKLDGVMPIGHRVAMLNPRLEGRTAGNSCSCIELAVEPGMVA
                     RVEESAVRFVAGEAASAEWGIAVRQGFRVPDDLRAYGRSAREAVLTREAAGITAERFG
                     ARLHGGRGVIGALAAVALIGLPHGVLLDPGREIAFGNGREIASPAETLMHEHNHIGTD
                     G"
     gene            6266..6502
                     /locus_tag="PLV90_07870"
     CDS             6266..6502
                     /locus_tag="PLV90_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoprotein"
                     /protein_id="HOF97069.1"
                     /translation="MVNGIVLPVKKVFSLVDSKIVVEIKDDGRKLQGRLIAVDEHLNL
                     HMDETTEYTGDQRGRTLGTVVIRGNNILTIAPLL"
     gene            6508..6849
                     /locus_tag="PLV90_07875"
     CDS             6508..6849
                     /locus_tag="PLV90_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOF97070.1"
                     /translation="MSDQEEEALKVIQSSRQGVLQSDLWKELEIDSRKCSRIVKRLLD
                     AGLIERVEFRSDGIKTYLLRAKKRAIDPCVILAGGEILPCIGCDQECVPEECALLLDW
                     MYQLALDEYQG"
BASE COUNT         1293 a         2267 c         2190 g         1128 t
ORIGIN      
        1 ggggtgagac agcagtcgat cgcgtcggcg tcgatagaga ggtagaccct cctcccgcca
       61 aactcgccga gcacctcgcc gaggacggcg gcgatccccc tctcgcgcac cgcgtcagcg
      121 gtatacagcc gcgtcctctc cgccgcaacc tcgaactgcc cggcatcgcc gctccgggcg
      181 ccgatgatca cgacgtcgtc gaccgtatcg aggacgcgcc gggtgacgca gccgtggctg
      241 taggccgtcc cgtcgagctc gtcgcgcagg tccaggtgcg catcgcagac gacgtagacg
      301 tccgggcgga cagccctgac cgcccctatc gtcgcggtgt gctcgccccc gagcatcacc
      361 ggcaccttgc cgtcgcggac gatgtccccc gtgacgtcgg ccacctggcc gaccaggtcc
      421 tccggcagcc gcaacaccat aagatcgccg aggtcgacga ttggcacctc ggaaaagtct
      481 actccggtcg aggggtcgta gggctcgaag ttgaacgagg cctcccggat agcccgggga
      541 ccaaaccgcg tccccggctt aaacgacgtc gtcgcgtcgt acggcacgcc gaagatggcg
      601 tagcgggcgt cctcgtagga cgcatccgca tccgcaaaat ggaggtgggc aaggtcgtgc
      661 atgcccgtct tcgagagcca gaatacgtca ctcaagcttc ttcttgccca gggatgctat
      721 gtaagcgatc tccttgcccg gctcaaggcc ggcggcctga tcctcggtca cggcaagctc
      781 gaacatatca aagtccttga catccatgag ctggacggtc gtgccggcga tcgatatgac
      841 ctgtgccatc tttcgctcca ccacgggaac gtaggtcttt gccgagaccg gctggacgat
      901 cgagcgtttg acgccgtcaa agatccctat gcagtcgatc cgggacttgg ctgccccgtg
      961 cttcccgggc ttggatatag cgatggagac aatcctgcaa ggctcctcat cgacgacgac
     1021 gtagcgcccc tctttcagtt tcccaacttc tgtctgttcc ttcatattgt ctcactactc
     1081 aacaaacgcg tgattaatgt atttagattg cagtacataa atacaccgta gaataaagag
     1141 gaggcgcaaa ggttctgtga tggagttcat ccgggcatgc gacgatctgg ccggggctgt
     1201 ctccgacgcc gtagccggac tggtcggaac gcccgaagca gggaggtgcg tgaggatggg
     1261 cgcggatgga acgcccacga aactgatcga ccaggtagcc gaggatatcg tcgtcgacta
     1321 cttcacgcag aacccgttct gccgccgcct gatcagcgag gagctcgggt gcgccgatat
     1381 gggcggagat acgggcacca tcttcctcga cccggtcgac ggcacctaca acgccgtcgc
     1441 cggcatcccc ttctacgccc tctccatagc ctacgcggaa ggcggcatcg tccaggccgg
     1501 atacgtcaaa aaccttgcca cgggcgagac gtttcacgcc gtccgggggc acggcgccta
     1561 tctcgacgga caccccatcc gggtctcgga gagagccctc ctcgagaaga gcgccatgag
     1621 cgtctacggc cggaagttcg acccgacccg cgtgctcggc atcaaccggc agatccggcg
     1681 ttggcgcctc ctcggcgcat cggcgctcga actctgctac gtcgggtgcg gccgcataga
     1741 cggctttgtc gacgtgcggg ggacgctccg cgtcacggac gcggcggccg ggatgctggt
     1801 ctgcgaagag gccggcggca ccgtctcgga cctcgagggg aacgccctcg tcttccctga
     1861 cgaggtatcg gtcgggcgga gcctggtcgc cacgaacggg atcctgcaca acaaggtcat
     1921 cgagtacctg aggtaggctg ccatgaagat cgtgctggta tcacgtatcg acgactccga
     1981 tgcgctccgc tacaccgccg acctcgcgag agacctcgag gccctcgggc acgacgtcgc
     2041 cttcgaagag ggcacggcac ggcacctcgg gaaggaaggc gtcccgttcg agagtatagc
     2101 gggcgacctc gtcgtggtcg tcggcggcga cgggtcggtc ctcctcaccg tccaccagat
     2161 gagaaagcag gtcccggtcc tcgggatcaa ctggggcgag gtgggattcc tcgcggacct
     2221 cgagcccgac gaggcccgcg cgttcttttc cgccgaaaag accgcgctcc gcgtcgagcg
     2281 gcggctccgg gtcagcctct ccgtcgacgg gaggtggctc ggcaacgccc tcaacgaggc
     2341 ggtcatcgtc accgaccggc cggcaaagat gctccggttc ggcgtttacg tcgacgggac
     2401 gccggcagaa cggttcaggg ccgacggcct gctcatatcc acccccaccg ggtcgaccgc
     2461 ctacgccatg agcgcgggag ggccgatcgt cgacccgcag atcgaggggt tcctcctcgt
     2521 gccgctcgcg ccctacatgc tctcgtcgcg cccccacatc atcagcaccg ggaggaacct
     2581 cgagatcacc cttgagacgg agaaaccggc gcacctcgtc atcgacgggc agagcacctt
     2641 cgagctcgac cgggaggcca cgatcagggt gaagaaggcg gacgagcccg ccctcttcgt
     2701 gtataccggg aaacccttct ttgagaaggt gaaccacaaa ctccgcaacc tctgataccc
     2761 aaacggataa tttatgggtg gcggggagga ttatctcctg aggagcacga tcatggaaga
     2821 ccagatgcgc acgaaactcc cttacgcgga gatagcagag acgctgaaga cgaccctcga
     2881 cctccggggg tcgccggtcg cggtgaaact tgcaaagagc cccgagggca tccccgaggg
     2941 ggtcaaaccg atcgaggaga cggtccgcca ctgccagatg gtcagcaagg cgaggcttga
     3001 gggtgcgatc ttttacgcga ccaccgacaa gcacgtctgc atgggcggcg gctgggccct
     3061 cgggctgaag gagctcacga ggagcctccg cacgggggag ttctactaca agctcggcaa
     3121 gttcgagagc tgggccgcct gcatgcggac catccggcag gtgccccacg tcccggagct
     3181 cgagacctac gcgacggtct acgcgccgct cgagaagacg ccgtttgacc cgcacgtcgt
     3241 catcatcatc gctgagccgc ggacaatgct gaagctcgcg caggccaccc tctaccacct
     3301 cggcgggcgg atagagtcca cgatgtcggg gatccagtct gtctgcgcgg acgcgaccgc
     3361 gctgccctac ctcaccggca agatcaacta ctccctcggc tgcgacggtt cgcgccggtt
     3421 ctccggcatc gaggataacg agctcgtcat gggcatcccc gccgagatcc tccccgaatt
     3481 tgcccgggcc cttgagatcg tcaccgcggc gcccggatcg gtgcggtaac cggcaccgct
     3541 catccccgct ctttttccca cgcctctccc ggggtacaag gcttatcttc ccggccggtc
     3601 aaccattcag caggtggttt tttgcaggta tccatgaagc tcgagatgag ggaccagccg
     3661 ggacagctgg tcgcggccct caaaccgatc tcggccgtcg ggggaaacat catagcagtc
     3721 attcaccaga gagagacctc gacacccgct gaagcgctgg atgtccagat cgtcctggag
     3781 ctgccggaag ggcgcctccc gaacctcctc gaactcttcc gcgcgcaggg catcaacgtc
     3841 gtgcgcatcg gcgaggagcg gctcctctac gagtgcacgc tgatcatgat cgggcacctg
     3901 atgcatacgg acctctccga cacggtcgac cggatcgaca ggaccggttc ggccgaggtg
     3961 acggaactct cgctcgtcat gccggcgatc gacaccccct cgtcggcccg catcaccatc
     4021 cgctcgacca cccgggcgga gatgaagaaa gcgatgcgga tccttcgcgc cgttgcgaaa
     4081 caaaaagatc tcctcatcat cgagcccctg gaggatgcat gatgcggatc gccatactcg
     4141 ggttcggatc cgtcggtcgg ggcatcgccc gggttctcct tgcaaaagat ctcggcatcg
     4201 ccgtcaccgg tcttgccgac tcaaagagcg gcatcatcga cgccggcggc atcgaccttg
     4261 cggcggcgct cgcccggaag gaaggaggcg gcccctgcgg gaacccgggc gtcagccccg
     4321 cggacgtcgt ggcaaaggcg gagtacgacg tcctcgtcga ggtgaccccg acgaacgtgg
     4381 agaccggcga accggcgctg ggccatatcc gggaagccct ccggcggcac aaacacgtcg
     4441 tcacatcgaa caaaggcccg atcgctctcg actaccccgg gctccgggcg ctcgcagagg
     4501 caaacggcgt cctcctgaag tacgaggcga cggtctgcgg cgctatcccg ctcatccacg
     4561 cgatgcagga agggcttgcc gggaacacca tatcccggct ctacggggtc ttcaacggca
     4621 cctgcaacta catactcacc cggatggccg cagaaggcct cacctacgag caggcccttg
     4681 ccgaagcccg ggaactcggg tatgccgagg cggacccgac ctacgacgtc gaggggatcg
     4741 acgcaggcat caagctggtc atactcgcca acaccatcct cgacctctcc acgacgcttg
     4801 acgacgtgga gaggacgggt atcaccctcc tctcgcccga ggcgctgcag cttgcagagg
     4861 accaggactg caccatccgg ctgatcggcg agatcatacc ggacgccggg gtgctccgcg
     4921 tatcgccgcg gataataccc aagactcatc cgctcgtcgt cgaaggaacg ctcaacgccg
     4981 tcaccgtcga ggccgacctc gccggggacc tcacgttcat cgggaagggc gcgggctcca
     5041 ccgagacggc cagcgccgtg atcggcgatc tgctctacat ccgtgagagg tatgtcaagg
     5101 gttcttgaac ggagaaaaca gtacctgcgg ctgatgcgtc aggtgacgct cgagaggggc
     5161 tccttcacgg tggccgatat cgcggaggcc accgacaccc cccggagcac cgtccaggac
     5221 tgggtgaacc gcctcgtcga ggagggatgc gtcctcctca cggaagagca gcggggccgg
     5281 cacgccgccc actacgcggc aagcagcgtg atgccggaga gcgcctgccg acgggtcttc
     5341 accaccgtcg acggcggcga ggtcgagatc taccacgagt gcatgagcgg cggatgcgcc
     5401 gcgttctgcg agttccacca tgcccgcgcc ggcggcgccc tgcagtcggt tcgccgggac
     5461 gggaccctgc tccgggagcg ggcggtcctc gggcggcggg acgtcgccgt cggcctcgac
     5521 cccgcgccgg cggtcgggat cgtcggcgtc tttcacgaag gcgactacat ccgccagcac
     5581 atcaggtgca tcgggggccc ggcctactcg ctcaccgaca tgatgtcctt tgccgagggt
     5641 gtctgcggcg tcaccgtccg ccgggagggg gcggtggtcg agggcgaggt gatcacccgg
     5701 gcgctcgcct acgtcgccat cggtatcgac gataccgata ccggggatga gggcgcgacg
     5761 ttcgccctcg cccttgccct cctccagcac cttgcaaaac tggacggcgt catgccgatc
     5821 ggccaccggg tggcgatgct caacccccgg ctcgagggcc ggacagcggg gaactcctgc
     5881 agttgcatcg agcttgcggt ggagcccggc atggttgcac gggtcgagga gtccgccgtg
     5941 cggttcgttg ccggtgaagc ggcgtcggcg gaatggggga tcgccgtccg gcaggggttc
     6001 cgggtgcccg acgacctccg ggcctacggg aggagcgcga gagaggcggt gctcacccgg
     6061 gaagcggccg gcataaccgc agaacgattc ggggcgcgtc tccacggcgg ccggggcgtc
     6121 atcggggcgc ttgcggccgt cgcgctcatc gggctcccgc acggcgtcct gctcgatccg
     6181 gggcgggaga tcgccttcgg gaacgggcgg gagatcgctt ccccggcaga aaccttaatg
     6241 catgaacata accatatagg aacagatggt taacggcatc gtactccccg taaagaaggt
     6301 tttttcactt gttgactcaa aaatcgtcgt tgagatcaaa gacgacggca ggaaactcca
     6361 gggtcggctc attgcggtgg acgaacacct gaacctgcat atggatgaga ccaccgagta
     6421 taccggtgat cagaggggcc gcaccctcgg gaccgtcgtc atacgaggca ataacatcct
     6481 gaccattgca cctctgctct gatagccatg tccgaccagg aggaagaagc actcaaggtt
     6541 atccaatcct ctcgtcaggg ggttctccag agcgatctct ggaaagaact tgagatcgat
     6601 agcaggaagt gctcaaggat cgtgaagcgg ttgctggacg ccgggctgat cgagcgtgtc
     6661 gagttccgca gcgacggcat caagacgtac cttctgcggg caaagaagcg ggcgatcgac
     6721 ccctgcgtca tccttgcagg gggggagatc ctcccgtgca tcggttgcga ccaggagtgc
     6781 gttccggaag agtgcgcgct cctccttgac tggatgtacc agctggccct cgacgaatac
     6841 cagggttgac ccgccccggc gggagacccc accccacc
//
LOCUS       DAOSII010000100         6594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_10589_length_6594_cov_51.038232, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000100 DAOSII010000000
VERSION     DAOSII010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6594
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10589_length_6594_cov_51.038232"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2439
                     /locus_tag="PLV90_07880"
     CDS             <1..2439
                     /locus_tag="PLV90_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HOF97071.1"
                     /translation="LVRAVNPTVKINRARFSAITPAEIRTAFENLTDLDFALAAAGEA
                     RQTVDLMWGASLTRFISLAARRGGKDILSVGRVQSPTLAMIVDRERAIENFVPQTYWM
                     LSLLTEKDGVPVEARHTAGRFTDHDAALAAEAGTKEPLTVTDVKEGQKTDRAPTPFDT
                     TTFIVAASRLGLSAANAMRIAEDLYMNGYISYPRTDNTVYPKSLNLNGILETLRGGVF
                     DADVAWVQQHRRSVPTRGKKESTDHPPIHPTGAATREALGQDRWKVYELVVRRFLATL
                     SPDATWTTTKCLFDASGEPYTATGGRLLSAGWRRVYPYSEAKENILPVFAVGERLPIR
                     KVNLEEKQTQPPARFSQSRLIQVMEELGLGTKSTRHEVIGKLISRRYVEGNPLRPTLV
                     GRAVTDALDNHAETITEPEMTRTLEEHMQLIKQRERSRDDVVDESREMLHRVFDKLEA
                     HEKEIGDEIMGQTAEEHTVGPCPACGHELRIRHLGASQFIGCTGYPECRFNISLPGST
                     WGKAIRTDEVCKTHKLTHVRLIRKGSPPWTIGCPLCSHIASNIEALRMMPSMTDSLVE
                     RLHAHHIYTVSEIAGMQPHDLAGAAGVGAEEAGQLIREAGGALDILRRRSELRKFIRK
                     IVPPKKGRSHAKITKSLVEQGVGDIRALSRSDSAVLKKAGIGETPAAELLAAARGLCN
                     ERTLREAGIPAVSLKKYQAGGIASPDDLCYLPVPYLANKTGINPETVHKHADMVCRHL
                     GRPAPEKVTKTALERGRKELLAVPGIGEATVEKLYLAGIYDAATLREADPGEVSSITG
                     IAKTRIHDYISHLK"
     gene            2444..3136
                     /locus_tag="PLV90_07885"
     CDS             2444..3136
                     /locus_tag="PLV90_07885"
                     /EC_number="2.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerol geranylgeranyltransferase"
                     /protein_id="HOF97072.1"
                     /translation="MHANWKTWAHVTKLDPDKRLPPGAAEEIATSGTDALMLSGTLNV
                     TQENLNELLDQVSPYGLPLVVEPASPDCAIFDGRIDHLFVPSVVNANDVRWIVGKHYT
                     WLRHANSIDWDMVVPEAYIVLNPNSAVGRVTGADCALSCRDVAAFVEVADRYFRFPIV
                     YIEYSGRYGDPSIVQAASDAVEHAVLYYGGGIRSAEQAAEMGRIADTIVVGNAVYDEG
                     IDVLRATVRAVQ"
     gene            3140..3838
                     /locus_tag="PLV90_07890"
     CDS             3140..3838
                     /locus_tag="PLV90_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOF97073.1"
                     /translation="MPEVSIVLVEPLYEGNVGFTARVMKNFGFTRMVLVNPCALGDDA
                     IMRAAHARDVLDGARRMTIDDVYREFDFVVATTGEVSKSVCTPMRMPYYAPAEVREAV
                     ADIDGTVAILFGRENWGLANTELARANFICTIPTSEAYPILNLSHAVGILCYELANLP
                     RGTYLLAGQVEMESLYRHIETFLDRIDHPDFKRENTMVLLRRVLGRTKLTVREVSTMH
                     GLMRRTEWHLENKE"
     gene            3862..4419
                     /gene="dcd"
                     /locus_tag="PLV90_07895"
     CDS             3862..4419
                     /gene="dcd"
                     /locus_tag="PLV90_07895"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOF97074.1"
                     /translation="MILVDWQIEDRIRRGYIRVDPYDPALIQPNSLDIRLGSHFVWYV
                     PGDIVIDPYERESVCADVEEVVADSIVLAPGQFLLAKTLEAIELPDDVVASIEGKSSI
                     ARLGIELHQTGGWIDAGFQGTITLEMCNVNSRPVRVYAGMPIGQLVFYRTDRAARPYN
                     MKKDAKYMGQQQATLSRYHENARHA"
     gene            4557..6392
                     /locus_tag="PLV90_07900"
     CDS             4557..6392
                     /locus_tag="PLV90_07900"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOF97075.1"
                     /translation="MRLLLIHSDHIEYEARKKTKVAEEDAVPKDALDEALAVFCAVES
                     VDEENIGDAVRQAADEIVTTARQLGTTNIMIYPYAHLSSDLASPEAAVSALRGIEEAL
                     SGESDFVVKRAPFGWYKAFSLSCKGHPLSELSRTIVPGEGTAAPKKEIEHEFFVFTPE
                     GERKDAAAYVDEKTPFAALIRKELGYPGPEGVEPVHVDLMRAKELVEYEPRSDVGHHR
                     WMPRGKLIRDLLTDYVLTQVLEYGGMPVETPVMYDLGDQAIAEHAAKFGERQYRFKSG
                     NRDMMLRFAACFGMFSIMHDMHISPNTLPMKLYELSTYSFRHEQKGEVIGLKRLRAFT
                     MPDMHTLCRDVDDALASFEEQLLMGWKSGEDLETPLVGVFRCTQDFFDQYELWVKGIV
                     AKSGVPMLIEVLSERTHYWIAKVDLAAIDAQGRPIENPTVQIDVESADRFDIKYYTPE
                     GVEVHPPILHCSPTGSIERVICALLENTAQMEVPSLPTWLAPTQVRLVPVAGRHICFA
                     EEIGARLNAAGIRADVDDRDESVNKKIREAGMDWAPYVAVIGDQEAETGRLTVTIRKL
                     SEKKKPYKEMMSEDELIEAVKMETAGKPSRPLYTPRLLSMKPRFI"
     gene            complement(6385..>6594)
                     /locus_tag="PLV90_07905"
     CDS             complement(6385..>6594)
                     /locus_tag="PLV90_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97076.1"
                     /translation="PPPRLSRGKGSARSDSRHGPCIAFSPLDRFAPPRHRRVWPPEGA
                     MPDGGCAQASAAHSISKRFAKKEVI"
BASE COUNT         1296 a         2156 c         2126 g         1016 t
ORIGIN      
        1 ctcgtccgcg cggtgaaccc cacggtcaag atcaaccggg cccggttctc ggcaatcacc
       61 cctgcagaga tccggaccgc ctttgagaac ctgaccgatc tcgacttcgc cctcgcggcc
      121 gccggcgaag cccgccagac ggtcgacctg atgtgggggg cgtcgctgac ccggttcatc
      181 agccttgccg cgcggcgggg cggcaaagac atcctctccg tcggccgggt ccagagcccg
      241 acgctcgcta tgatcgtcga ccgcgaacgc gcgatcgaga acttcgtccc ccagacctac
      301 tggatgctct cgctcctgac ggagaaggac ggcgtgccgg tggaggcgcg gcataccgcc
      361 ggacggttca ccgaccacga tgcggccctt gccgccgagg cggggacgaa agagcccctc
      421 acggtcaccg acgtcaagga agggcagaag accgaccggg cgccgacacc cttcgacacc
      481 acgacgttca tcgtcgcggc aagccgcctg ggcctctcgg cggcaaacgc catgcggatc
      541 gccgaagacc tctacatgaa cgggtacata tcctacccga ggaccgacaa cacggtctac
      601 ccgaagtccc tgaacctgaa cggcatactc gagacgcttc ggggaggggt cttcgatgcg
      661 gacgtcgcct gggtgcagca gcaccgccga tcggtcccga cccggggcaa gaaggagagc
      721 accgaccacc cgccgatcca cccgaccgga gcggcgaccc gcgaggccct cggccaggac
      781 cggtggaagg tctacgagct cgtcgtccgg cggttccttg cgaccctctc gccggacgcg
      841 acctggacga cgacgaagtg cctcttcgac gcctccggcg agccctacac cgcgacgggc
      901 gggcggctcc tctccgcggg atggcgccgg gtctacccct actcggaggc aaaagagaac
      961 atactcccgg tatttgccgt gggcgagcgt ctgccgatcc ggaaggtgaa tctcgaagag
     1021 aaacagacgc agccgccggc acgcttctcc cagagccggc tcatccaggt gatggaggag
     1081 ctcggcctcg gcacgaagag cacccggcac gaggtgatcg ggaagctcat atcccgccgc
     1141 tacgtggaag gaaacccgct ccgcccgacc ctcgtcggga gggccgtcac ggatgcgctc
     1201 gacaaccacg cggagacgat caccgagccc gagatgacga gaacgcttga agagcatatg
     1261 cagctcatca agcagcgcga gcgctcccgc gacgacgtcg tcgacgaatc cagggagatg
     1321 ctccatcggg tctttgacaa gctcgaggcg cacgagaagg agatcggcga tgagatcatg
     1381 ggccagacgg cggaagagca taccgtcggc ccctgccccg cctgcggcca cgagctccgg
     1441 atccggcatc tcggcgcttc tcagttcatc gggtgcaccg gctacccgga gtgccggttc
     1501 aacatcagcc tgcccggcag cacctggggg aaggcgatcc ggaccgacga ggtctgcaag
     1561 acgcacaaac tcacccacgt tcgccttatc cggaaaggat cccccccgtg gacgatcggc
     1621 tgcccgctct gcagccatat cgcctcgaac atcgaggcgc tccgtatgat gccctcgatg
     1681 accgacagcc tcgtagagcg cctgcacgca caccacatct acacggtatc cgagatcgcg
     1741 ggcatgcagc cccacgacct tgcgggtgcc gccggcgtcg gcgcggagga ggccgggcaa
     1801 ctcatccggg aggccggggg cgcgctcgat atactccggc gccgctcgga gctccggaaa
     1861 ttcatcagga agatcgtccc cccaaagaag ggccggagcc acgcgaagat aacaaaaagc
     1921 ctcgtcgagc agggggtcgg ggatatccgc gcgctctcgc ggtctgactc tgcggtcctg
     1981 aagaaagccg gtatcggcga gacgccggcg gcagagctcc tcgcggcggc ccgcggcctt
     2041 tgcaacgagc gcaccctcag ggaagccggc attcccgcgg tcagcctgaa gaagtaccag
     2101 gcagggggca tcgcaagccc cgacgacctc tgctacctcc cggtccccta cctcgcaaac
     2161 aaaaccggca ttaatccgga gaccgtacat aaacatgctg atatggtctg caggcacctc
     2221 gggcgccccg ccccggaaaa ggtcacgaag accgcgcttg agcgcgggcg aaaagagctc
     2281 ctggcggtcc ccggcatagg ggaagccacc gtggagaaac tctaccttgc gggcatatac
     2341 gacgccgcga cgctccgcga ggccgacccc ggggaagtct cctccatcac cggcatcgcg
     2401 aagacgagga tccacgacta catcagccac ctgaagtgac accatgcacg cgaactggaa
     2461 gacctgggca catgtgacga agctggaccc cgacaaacgc cttccaccgg gcgccgccga
     2521 ggagatcgca acgagcggga ccgatgccct gatgctctcg ggcaccctga acgtgacgca
     2581 ggagaacctc aacgagcttc tggaccaggt ctccccctac gggctgccgc tggtcgtgga
     2641 gccggcgagc cccgactgcg ccatattcga cgggcggatc gatcatctct tcgtgccgag
     2701 cgtggtgaac gcaaacgacg tccgctggat cgtcgggaaa cactacacct ggctccgcca
     2761 cgcaaacagc atcgactggg atatggtggt gcccgaggcc tacatcgtcc tcaatccgaa
     2821 ctccgccgtc gggcgagtga cgggggcgga ctgcgccctc tcgtgcaggg atgtggccgc
     2881 attcgtagag gtggcggacc gttacttccg gttccctatc gtctacatcg agtacagcgg
     2941 aagatacgga gacccgtcga tcgtgcaggc ggcatccgat gcggtcgagc acgccgtcct
     3001 ctactacggg ggcggcatcc gttcggcgga gcaggcggcg gagatgggcc ggatcgcgga
     3061 tacgatcgtc gtcggcaacg ccgtctacga tgaggggatc gatgtgctcc gggcgacggt
     3121 ccgggcggta cagtgaggca tgcctgaggt ctcgatcgtc ctggtcgaac ccctctacga
     3181 ggggaacgtc gggtttaccg cccgggtgat gaagaacttc gggttcaccc ggatggtgct
     3241 ggtcaacccc tgcgcgctcg gcgacgacgc catcatgcgc gccgcccatg cccgcgacgt
     3301 cctcgacggc gctcgccgga tgacgatcga cgatgtctac cgggagttcg acttcgtcgt
     3361 cgcgacgacc ggcgaggtga gtaagtcggt ctgcacgccg atgcggatgc cctactacgc
     3421 gccggcggaa gtccgggagg ccgtcgccga tatcgacggg acggtcgcga tcctcttcgg
     3481 ccgggagaac tggggcctcg cgaacaccga acttgcgcgg gcaaacttca tctgcaccat
     3541 cccgacatcg gaggcatacc ctatcctgaa cctctctcac gcggtgggca tcctctgcta
     3601 cgaactggcc aacctgcccc gcgggaccta cctccttgcg gggcaggtcg agatggagtc
     3661 gctctaccgg cacatcgaga cgttcctcga ccggatcgac caccctgact tcaaacggga
     3721 gaacacgatg gtcctcctcc ggcgcgtgct cggccggacg aaactgaccg tccgcgaggt
     3781 gagcacgatg cacgggctta tgcgccggac ggagtggcat ctcgaaaaca aagaataggt
     3841 tcgaggagaa gagctaaagg aatgattctt gtcgactggc agatagagga ccgtatcagg
     3901 cgcgggtata tacgggtcga cccctacgac cccgccctga tccagcccaa ctccctggat
     3961 atcaggctcg gttcccactt cgtctggtac gtgccggggg atattgtgat cgatccctac
     4021 gagagggaga gcgtctgcgc agatgtcgag gaggtagtgg ccgactcgat cgtcctcgcg
     4081 ccggggcagt tcctccttgc aaagaccctg gaggcgatcg agctccccga cgacgtcgtg
     4141 gcgagcatcg aggggaagag cagcatcgcc cgcctcggta tcgagctcca ccagacgggg
     4201 ggctggatcg acgccggatt ccaggggacg ataacgcttg agatgtgcaa cgtgaactcc
     4261 cggccggtcc gggtctacgc cgggatgcct atcgggcagc tcgtcttcta ccggacggat
     4321 cgcgccgcgc gcccctacaa tatgaagaag gacgcgaagt acatgggcca gcagcaggcg
     4381 accctctccc gctaccacga gaatgcgcgc catgcgtgat gcatactgcc ggtagtccgg
     4441 aaaagacccc gcgcattcca gctccggcgg gcagggtgcc gggtgttccg cccggaagag
     4501 cgttatgcac tatatagcag gaaaacagat attcaacggt tcaactgggg ataccaatgc
     4561 gacttcttct gatccactct gaccatatcg aatatgaggc ccggaagaag acgaaggtcg
     4621 ccgaagagga tgcggtccca aaggacgctc tcgatgaggc gctcgccgtc ttctgcgcgg
     4681 ttgagtctgt cgacgaggag aatatcgggg atgccgtcag gcaggcggcg gacgagatcg
     4741 tcactaccgc ccggcagctc ggcacgacca atatcatgat ctacccctac gcccacctct
     4801 cctccgatct tgcatcgccg gaagcggcgg tgagcgctct ccggggcatc gaggaagccc
     4861 tctccgggga aagcgacttc gtcgtgaagc gggcgccgtt cggctggtac aaggccttct
     4921 cactctcctg caagggccac ccgctctcgg agctctcccg gacgatcgtg cccggggagg
     4981 gaacggccgc gccgaagaag gagatcgagc acgagttctt cgtcttcacc cccgaagggg
     5041 agcgcaagga tgcggccgcc tacgtggatg agaagacgcc cttcgccgcc cttatccgga
     5101 aagaactcgg gtatccgggg ccggaagggg tcgaaccggt tcacgtcgac ctgatgcggg
     5161 caaaagagct cgtggagtac gaaccgcgct ccgacgtcgg gcaccaccgc tggatgcccc
     5221 ggggcaagct gatccgcgac cttctcaccg actacgtcct tacgcaggtg ctcgagtacg
     5281 gcggtatgcc ggtggagacc ccggtgatgt acgatctcgg cgaccaggcg atcgcggagc
     5341 acgccgccaa gttcggggag cggcagtatc ggttcaagag cggcaaccgc gacatgatgc
     5401 tccggttcgc ggcgtgcttc ggcatgttct cgatcatgca cgacatgcac atatcgccga
     5461 acaccctccc gatgaagctc tacgagctct cgacctactc gttccggcac gagcagaagg
     5521 gcgaggtgat cgggctcaag cgcctccgcg ccttcacgat gcccgacatg cacaccctct
     5581 gccgcgacgt cgacgacgcg ctcgcaagct tcgaggagca gcttctgatg ggctggaaga
     5641 gcggcgaaga cctcgagacg ccgctcgtgg gggtcttccg gtgcacgcag gacttcttcg
     5701 accagtacga actctgggtg aagggaatcg tcgcgaagtc gggtgtgccg atgctcatcg
     5761 aggtcctctc ggagcggacg cactactgga tagcaaaggt cgaccttgcg gcgatcgatg
     5821 cccagggcag acccatcgag aacccgacgg ttcagatcga cgtcgagagc gcggaccggt
     5881 tcgacatcaa gtactacacc ccggagggtg tggaggtcca ccccccgatc ctccactgtt
     5941 cgccgaccgg ctcgatcgag cgggtcatct gcgcgctgct cgagaacacc gcgcagatgg
     6001 aggtcccctc gctcccgacc tggctcgccc cgacccaggt caggctggtc ccggtggcgg
     6061 ggcggcacat ctgctttgcg gaggagatcg gtgcacgcct gaacgccgcg ggcattcggg
     6121 cggatgtcga cgaccgcgac gagagcgtga acaagaagat ccgcgaggcc ggcatggact
     6181 gggcgcccta cgtcgcggtg ataggcgacc aggaggccga gaccggccgg ctcacggtca
     6241 ccatccggaa gctctcggag aagaagaagc cgtataagga gatgatgagc gaggacgagc
     6301 tcatcgaggc ggtgaagatg gagaccgccg gaaagccttc ccgcccgctc tacaccccgc
     6361 ggctcctctc aatgaagccc cggttcatat aacctctttt ttcgcgaacc tctttgagat
     6421 ggagtgagct gctgaagcct gagcgcagcc gccgtcgggc atggcccctt cgggcggcca
     6481 gacccggcga tggcggggcg gggcaaaacg gtcgagcggg gagaacgcga tgcaggggcc
     6541 gtggcggcta tcgctccggg cactgccctt accccgggat aggcggggcg gggg
//
LOCUS       DAOSII010000101         6556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_10690_length_6556_cov_37.981387, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000101 DAOSII010000000
VERSION     DAOSII010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6556
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10690_length_6556_cov_37.981387"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="PLV90_07910"
     CDS             complement(<1..961)
                     /locus_tag="PLV90_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOF97077.1"
                     /translation="MKQDATYIRWFIDVRNEDVGIVGGKNASLGEMYSELTPKGVKIP
                     DGFAVTADGYWHVLTSAGVLDDLKETLAGVDRSNVADLAKRGRKARDLILSAGIPDDL
                     WGEIKTAYDMLCDEYGPDADVAVRSSATAEDLPTASFAGQQETYLNIRGYQALREAYV
                     RCLASLFTDRAIAYRIDNHFDQFKVALSVGIMKMVRSDLASSGVIFTLDTDTGFRDVV
                     LITGSYGLGEMIVQGAVNPDEFYVFKPTFRKGYRAIVRKNLGEKRVKLIYGQGGAKEV
                     TRRVDVPDADQRRFCIADDDILTLAGYALTIEDHYSAKAGKPVP"
     gene            complement(958..2016)
                     /locus_tag="PLV90_07915"
     CDS             complement(958..2016)
                     /locus_tag="PLV90_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOF97078.1"
                     /translation="MVKLVFFDLDEHEQDVVRDAFAREGGYEIDLHPDALTSKNAPFA
                     RNADGIGVFVTSHVTREILDRLPNLKMVAAVSTGFDNIDVDACRERNIAVCNVPRYGD
                     NTVAEFAFGLILALARKFRPTFERTARGEFSRTGLQGMDIAGKTLGLIGTGHIGSHLA
                     RIAHGADMKIVAYDPHPKPDLTRDYGIRYLALDDLLRESDVISLHVPYTKATHHLINA
                     ERLQLVKNSAFLINTSRGGVVDTRAVATALNEGRLGGVALDTFEGEEVWIEEEFAGTG
                     EPPAAELKEALESFAILHSDRAILSPHNAFNTREALERILATSIENIRAFFAGNPQNV
                     VAGTYRVPAAPPAGGGRT"
     gene            complement(2025..3032)
                     /gene="gap"
                     /locus_tag="PLV90_07920"
     CDS             complement(2025..3032)
                     /gene="gap"
                     /locus_tag="PLV90_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOF97079.1"
                     /translation="MKKVAINGFGRIGRKVLSNYITDPPDNIAIVAVNDPNPTEELAY
                     LMKYDSVHGRAPFSIEAGSDRLKLGSKEVRVLHDRDPARLPWGDLGVDLVLECTGHFT
                     ERAGSAKHIDAGAKRVAISAPSPDADLTVVLGVNESAYDPAKHTIISNASCTTNALAP
                     AAKVLNDSFGVERLMATAIHAYTATQALVDRATKKPRRGRAAAVSLIPTTTGAAVATT
                     RVLPELTGKMDAIAIRAPIPDGSVVDIAAELKRETTTDAVNAAMKQAADGKMKGYLAY
                     TDDPLVSADIIGDPHSGVIDGRSTGVVDGRMARVLVWYDNEYGYAKRMVDIASYMASR
                     E"
     gene            complement(3303..4046)
                     /gene="gpmA"
                     /locus_tag="PLV90_07925"
     CDS             complement(3303..4046)
                     /gene="gpmA"
                     /locus_tag="PLV90_07925"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HOF97080.1"
                     /translation="MVELILLRHGESLWNRENRFTGWTDVDLSARGREEAHRAATLLG
                     GEHTFHVAYTSVLKRAIRTLWIVMDDLDLMYVPVQRSWRLNEKSYGALQGLDKPETAA
                     KYGTEQVHLWRRAYDVRPPPLSWDDSRHPRFDPRYADMDPETLPATESLHDTLNRVLP
                     YWESHITTDLRRGKPVLVAAHGNSLRALIKHLDNVPDGDIADLNIPTGYPLIYELDAD
                     LHPIRHYYLGDPEEVAAAARGVARQAGGR"
     gene            4277..5854
                     /locus_tag="PLV90_07930"
     CDS             4277..5854
                     /locus_tag="PLV90_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HOF97081.1"
                     /translation="MAPNRRRNKNYILATAALLIVVVAAIIAAGCTAGTSTPGAVIGL
                     TGVSWSLDSYCDENSTLVGVLQGTEITARFDDGKVTGLAGCNQYGGDYHQNGTALSFS
                     TLAQTLKYCEEPRGIMEQEARFMEVLGSTAGYRIENDRLLLTDAAGATVLVFTKEKPA
                     SIVGTSWTLAGLAGEDGGLVPVVPGTTVTAVFGADGRIGGSAGCNRYGANYAVHGADL
                     TIEPTTMTEMYCGEPSGVMEQEDRYLALLAEVASCRVEGDRLILTDANGTDILVFEPI
                     IRAPDLPLAGTDWVLDGYSTGKDAVSPVIAGTEITAMFAPDGKVAGSAGCNHYGGSYS
                     LDGGNLSVSSLFSTLMYCGEPEGIMEQEGKYLALLANVSSYRVQGDRLTLTDAEGTDL
                     LFFVVAEEVPPAPLAGTNWVLDTYSTGEDAVSSVIAKTTITVTFSPDGNIAGNAGCNN
                     YGAAYRLDGTNLSVSPPVSTKMYCGEPEGIMEQEARYLTLLESVAGYRIDGNRLDLLD
                     GAGGILLSFRADDADRS"
     gene            6046..6552
                     /locus_tag="PLV90_07935"
     CDS             6046..6552
                     /locus_tag="PLV90_07935"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HOF97082.1"
                     /translation="MRDGHHALAKAAFLIIVAACLVSAGYSGDTSPGDAGQAAEPLMG
                     TAWNLVGYRADNGSVVAPLPGKEPSVIFGGDGTLSVSAGCNLYSTSYRIDGSRIMVEP
                     ATAAGTHPAVEQDIARQESRYWELLLAAASYRVEGNRLVITGLSGQDLLAFSRAGEPA
                     DVPLLAME"
BASE COUNT         1115 a         2051 c         2093 g         1297 t
ORIGIN      
        1 tcggcaccgg ttttccggcc tttgccgagt agtggtcctc gatcgtgagc gcataccctg
       61 caagcgtcag gatatcatcg tcggcgatac agaaccgtct ctggtcggca tccgggacat
      121 cgacacgccg ggtgacctcc tttgcacccc cctggccgta gatcagtttc acccgcttct
      181 cgccgaggtt cttcctgacg attgcccggt aacctttccg gaacgtcggt ttgaagacgt
      241 agaattcgtc ggggttgacg gcaccctgga cgatcatctc gccaagaccg taggatccgg
      301 ttatgaggac gacgtccctg aaaccggtgt cggtgtcgag cgtgaatatg accccgcttg
      361 acgcgaggtc ggagcggacc atcttcatga tcccgaccga gagggcgacc ttgaactggt
      421 cgaagtggtt gtctatccgg taggcgatag cccggtcggt gaagagagag gcaaggcatc
      481 tgacgtaggc ctcccgcagc gcctggtagc cgcgaatgtt taagtaggtc tcctgctggc
      541 ctgcaaacga ggccgtcggg agatcctcgg cagtggccga actccggacg gcgacatcgg
      601 cgtcgggccc gtactcgtcg cagagcatat cgtaggccgt cttgatctcg ccccagaggt
      661 catcgggtat cccggccgag aggatgaggt cacgggcctt ccttccccgc ttcgcgaggt
      721 cggcgacgtt gctcctgtca acccccgcga gggtctcctt caggtcgtcg agtacccctg
      781 cggacgtgag gacgtgccag taaccgtcgg cagtcacggc aaatccgtcg ggtatcttca
      841 cgcctttcgg tgtcagttcg ctgtacatct cgccgagcga tgcgttcttc cccccgacga
      901 tgccgacgtc ctcgttcctg acgtcgatga accagcggat gtaggttgca tcctgcttca
      961 tgttcttcca cctccggcag gcggcgccgc cggcacccgg taggtcccgg caacgacgtt
     1021 ttgggggttt ccggcaaaga aggcccttat gttctcgatg ctggtcgcaa gtatccgctc
     1081 aagcgcctca cgggtgttga aagcgttgtg cggcgagagg atcgcccggt ccgagtgcag
     1141 gatggcgaag ctctcgagcg cctccttgag ctcggccgcc gggggttcgc ccgtcccggc
     1201 aaactcctcc tcgatccaga cctcctcgcc ctcgaaggtg tcgagcgcga cccccccgag
     1261 gcggccttcg ttgagggcgg tcgcgaccgc ccgcgtatcc acgacaccgc cccgggatgt
     1321 gttgatgaga aacgcgctat tcttgaccag ctgcaaccgt tcagcgttga tgaggtggtg
     1381 cgtggccttc gtgtagggga cgtgcaggct gatgacgtcg gactctcgca gaaggtcgtc
     1441 gagggcgagg taacggatgc cgtagtcccg ggtgaggtcg ggtttcgggt gtgggtcgta
     1501 ggcgactatc ttcatatccg ccccgtgggc gatgcgggca aggtgcgatc cgatgtgccc
     1561 ggtcccgatc aggccgaggg tttttccggc gatgtccatg ccctgaaggc cggtccggga
     1621 gaattcgccc cgggcggtac gctcaaacgt cggcctgaac ttccgggcaa gggcgaggat
     1681 gagcccgaac gcgaactccg ctacggtgtt gtcgccgtaa cgcgggacgt tgcagacggc
     1741 tatgtttctc tcccggcagg catccacgtc gatattgtca aacccggtgg agacggcggc
     1801 gaccatcttc aggttcggca accgatcgag gatctcacgg gtgacgtgcg atgtgacaaa
     1861 gacccctatc ccatcggcgt ttcgggcgaa aggcgcgttc ttgctcgtga gcgcatcggg
     1921 gtgcaggtcg atctcatagc cgccctcgcg ggcgaaggca tccctgacca catcctgctc
     1981 gtgctcgtcg aggtcgaaga agaccagttt taccatgggt cccctcactc ccgcgaagcc
     2041 atgtaggagg cgatgtcgac cattcttttt gcgtacccat actcgttgtc gtaccagacc
     2101 aggacccggg ccatccgccc gtcgacgacg cccgtggatc ggccgtcgat gaccccggag
     2161 tgcgggtcgc ctatgatatc ggccgatacg agcgggtcat cggtgtaggc gaggtagccc
     2221 ttcatttttc catccgcagc ctgcttcatc gcggcgttca ccgcgtcggt ggtcgtctct
     2281 cgcttcagtt ctgcggcgat atcgacgacc gacccgtcgg ggatgggggc ccggattgct
     2341 atcgcatcca tctttccggt gagttcgggg aggacgcggg tggtggcgac ggcggcgccg
     2401 gtcgtcgtcg gtatgagcga gacggccgcg gcacgtcccc ggcggggctt cttcgttgcc
     2461 cggtcgacca gcgcctgggt agcggtgtag gcatggatcg ccgtcgccat cagccgctcg
     2521 acgccgaacg agtcgttgag gacctttgcc gccggtgcga gcgcgttggt cgtgcaggag
     2581 gcgttggata tgatagtatg ttttgcgggg tcgtaggcac tctcgttgac gccgagcacg
     2641 accgtcaggt ccgcgtcggg cgagggtgcg ctgatggcga cccgtttcgc cccggcatcg
     2701 atgtgttttg ccgagcccgc ccgttcggtg aagtgtccgg tgcactcaag caccagatcc
     2761 acgccaagat cgccccacgg aaggcgtgcg ggatctctgt cgtgcagcac ccgcacctct
     2821 tttgacccca atttgaggcg atccgaaccg gcctcgattg agaagggcgc ccgcccgtgg
     2881 accgagtcgt acttcatcag gtaggcgagc tcttctgtcg ggtttgggtc gtttaccgcg
     2941 acgatcgcga tgttatccgg cggatcggtg atatagttgg agaggacctt ccgcccgatc
     3001 cgcccgaacc cgttgatggc aaccttcttc atgacacttc ccccctgtca gacaggtggt
     3061 ctcccctcta caggatggta tatatgatct tctaccaccg ggttctgccc cggaaagtga
     3121 gcgaagatgt cgcctcttca tgaaaaatct catttacgct ggagatgggt tcccggctgg
     3181 tgcgttcccg ggcacggttc gtcctgccgg atatcgccac gattgccccc cgccccccat
     3241 cggggcgaga ggtgagaggg aggagggtat cagcctctcc tgcggggagg ggccgccccc
     3301 ggctatctcc cccccgcctg ccgggcaaca ccccgggcgg ccgccgcgac ctcctcgggg
     3361 tcgcccaggt agtagtgcct gatggggtgc aggtcggcgt ccagctcgta gatgaggggg
     3421 tagccggtgg ggatgttgag atctgcgata tcgccgtccg ggacgttgtc caggtgcttg
     3481 atcagggcac ggaggctgtt tccatgggct gcgaccagga cgggttttcc cctccggaga
     3541 tccgttgtga tatggctctc ccagtacggg agcacgcggt tgagcgtgtc gtgcagggac
     3601 tcggtcgcag ggagggtctc cgggtccatg tcggcatagc gggggtcaaa ccgcgggtgc
     3661 cgggagtcat cccatgagag cggcggcggc cggacgtcgt atgcccggcg ccagaggtga
     3721 acctgctccg tgccgtattt cgcggcggtc tccggcttat ccaggccctg cagggcgccg
     3781 taactcttct cgttcaaccg ccatgaacgc tgcaccggga cgtacatgag gtcgaggtcg
     3841 tccatgacga tccagagtgt ccggatggcc cgtttcagta ccgatgtgta ggcgacatgg
     3901 aatgtatgct cgccgccaag gagcgtcgcc gcccggtggg cctcttctct gccccgggcc
     3961 gagaggtcca cgtcggtcca gcccgtgaac cggttctccc tgttccagag gctctcgccg
     4021 tgccgtaaga ggatcagttc taccattgca tatctctccc atacgaggag gcatccaccg
     4081 ggcctccacc catcgtggta gtcctccata ccggatataa tcgcccccgc ctccccctgg
     4141 caggggcggg gcataggagg ccggagccga tataacctct ataaaccggc cgtttaccaa
     4201 atcgggctcc tataagttcg atttttttcg tagcttacca tccatttatc cctcaatcgc
     4261 tcccatatgg tagcgtatgg caccgaacag aagaaggaac aagaactaca tactcgcaac
     4321 ggcggcgctc ctcatcgtcg tcgtggcggc aatcatcgcc gcggggtgca cggcgggcac
     4381 gtcgacaccg ggcgctgtca tcgggctcac cggggtctcc tggtcgcttg actcctactg
     4441 tgatgagaac agtacactcg taggagtcct gcagggaacg gagatcaccg cccggttcga
     4501 cgacggaaag gtcacgggcc tggccgggtg caaccagtac ggcggcgact accaccagaa
     4561 cggcacggct ctctcgttct ccacgctcgc tcagaccctg aaatactgtg aggagccacg
     4621 gggcatcatg gagcaggaag cacggttcat ggaggtcctc gggtcgacgg cgggctaccg
     4681 gatcgagaac gatcggctcc tcctcaccga cgctgccggg gcaacggtgc tcgtcttcac
     4741 gaaggaaaaa cccgcgtcga tcgtcggaac atcctggacg ctcgccggcc tcgccgggga
     4801 ggatggcggt ctggtaccgg tcgttcccgg cacaacggtc acggcggtct tcggtgccga
     4861 tggcagaatc ggcggatcgg ccggatgcaa ccgctacggt gcgaactacg ccgtacatgg
     4921 cgcggacctc acgatcgaac cgaccaccat gaccgagatg tattgcggcg aaccctccgg
     4981 cgtcatggag caggaggacc gctaccttgc gctcctggcc gaagtcgcct cctgccgggt
     5041 cgagggcgat cggctgattc tcaccgacgc taacggcacc gatatccttg tctttgagcc
     5101 gatcatccgg gcgcccgacc tcccgcttgc cgggacggac tgggtgctcg acggctacag
     5161 caccgggaaa gatgcagtat ccccggtgat cgccgggaca gagatcaccg ctatgttcgc
     5221 gcccgacggg aaggtcgccg ggagtgccgg gtgcaaccac tatggcggct cgtacagcct
     5281 cgacggcggg aacctctcgg tctcgtcgct cttcagcacc cttatgtatt gcggcgaacc
     5341 ggagggcatc atggagcagg aggggaagta cctcgcgctc cttgcaaacg tatcgtccta
     5401 cagggtgcag ggcgaccggt tgaccctcac ggatgcagag gggaccgatc tcctcttctt
     5461 tgtggtggcg gaggaagtcc cgccggcgcc gcttgccggc actaactggg tgctcgacac
     5521 ctacagtacc ggagaagatg cagtctcctc ggtgatcgct aaaaccacga tcacggtgac
     5581 gttctcaccg gacggcaaca tcgccgggaa tgccgggtgc aacaactacg gagccgccta
     5641 ccggctcgac ggcacgaacc tttcggtctc gccgccggtc agcacgaaga tgtactgcgg
     5701 cgagccggag gggatcatgg agcaggaggc gcgttacctc acccttcttg agtccgtggc
     5761 aggctaccgt atcgatggga accggctcga cctccttgac ggggctggag ggatcctcct
     5821 ctccttccgg gccgatgacg cggatcggtc ctgacccgac ctccgtcccc tttttccacg
     5881 tataccgctc gaggacggtt tttttgcccg ccggaaccat atccggatga gggctacaaa
     5941 acgacgcctc ctctgcaagc gctcaagaaa gatggcgatc gagagaatct cgatgaaaat
     6001 cggttatttt ttcttccctc agcccttacg cctaccgtgg taaatatgag ggacgggcat
     6061 cacgcacttg cgaaggccgc attcctgatc atcgtcgcgg cgtgcctcgt ctctgccggg
     6121 tactccggcg atacatctcc cggcgatgcc ggtcaggccg ccgaaccact tatggggacg
     6181 gcgtggaacc tggtcggata ccgtgccgat aacggctccg tcgtcgcgcc cctgcccggg
     6241 aaggagccat ccgtcatctt cggcggcgat ggcacgctct cggtgtccgc cgggtgcaac
     6301 ctctactcca cctcctaccg gatcgacggt tccaggatca tggtggagcc ggccaccgcc
     6361 gcaggcacac accctgcggt ggagcaggat atcgcccggc aggagagccg gtactgggaa
     6421 ctccttcttg cggctgcatc ataccgggtt gagggcaaca ggctcgtgat caccgggttg
     6481 tcgggccagg atctgctcgc cttctcgcgg gccggcgagc ctgcggatgt gccgctgctc
     6541 gcgatggaat agcggc
//
LOCUS       DAOSII010000102         6495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_10802_length_6495_cov_32.323292, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000102 DAOSII010000000
VERSION     DAOSII010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6495
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10802_length_6495_cov_32.323292"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PLV90_07940"
     CDS             complement(<1..287)
                     /locus_tag="PLV90_07940"
                     /inference="COORDINATES: protein motif:HMM:NF024736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOF97083.1"
                     /translation="MTFREIDDETWEIIRRHLPPRKPHVGRPRRDPRHLFNGILYVLS
                     TGCTWSDVPAKYGTKSTVHRYHLELCERGVYQAIFLDLLRSGYEFRKREVDR"
     gene            complement(376..1314)
                     /locus_tag="PLV90_07945"
     CDS             complement(376..1314)
                     /locus_tag="PLV90_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOF97084.1"
                     /translation="MATYTARPFLKWAGGKAQLLDAFTRRIPRELSEGTLPVFVEPFV
                     GGGAVYFHFNSTFRFRECHIFDINEELILTYSVVKSDVCALIDYLAGISDDYLTKDDL
                     GRKDFYYAMRDTFNRAKETIDFGQYGEGWIERAAQLIFLNRTCFNGLYRVNLQGGFNV
                     PFGRCRNPTILNEDVLRADAELLRNTTIHHGDFAQSEQYVSEETFVYLDPPYRPLNRT
                     STFTQYAKEGFTDEEQRRLAAYYARCDARGARLMLSNSDPKNVDPCDEFFDDLYAGYR
                     IDRVPARRMINCNGDGRGEIREIIVTNYDPLAPGER"
     gene            complement(1514..2545)
                     /locus_tag="PLV90_07950"
     CDS             complement(1514..2545)
                     /locus_tag="PLV90_07950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOF97085.1"
                     /translation="MRSEEINGNIFYNGDCIRGAREHIPDGTIDLIVTDPPYGIDGDR
                     LHRHYNRDEAFVVEGYVEVPAAGYGEFSRQWIREAERILRPGGSIYIVSGYTNLYHIL
                     HALRQTELREVNHIIWKYNFGVYTSRKYVSSHYHILFYEKPGGKRTFNLESRYGTGEK
                     APDNGSPNYRDREDVWVVNREYKPGKAKNKNELPAELLVKMLQYSSNEGDLVCDMFLG
                     GFSTARAAVGLNRRATGFELSGPVFDLKVKELRGIGQGCLLSSLRTPLASGPKNRGRP
                     WTEDEIEALVSRFARLIEGGATKKKAVDTLAEELGRGRWAIEKMLKREGVRRTGRGAT
                     TPAPRSREE"
     gene            2888..>4479
                     /locus_tag="PLV90_07955"
     CDS             2888..>4479
                     /locus_tag="PLV90_07955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF97086.1"
                     /translation="MTETSSRTGAGEREQKTSAMGEGPVHECIAAILAENEALRRENA
                     LLQSMKNALEEGEMRYRRLFEDALTGDFITAPDGRIIACNPAFARMFGFSSVEGALCT
                     NVRDLYEDPGDRDRLLAQLRRGGRVVNEGRTRKRRDGTRIHVVENVVGRLGADGELIE
                     IQGYTYDDSERKRVEDALRESEERFRAVLENSLDSAYRRNLQADCYDYMSPAIEEILG
                     FTPEEMAVMSLEEVMDRIHPDDRSLVKAELNTAAASGRGLLVYRFLAKGGEYRWLEDH
                     FTVISDSAGRPLFRGGIVRDITGRRRAEEAIARSAAELARVHRDLESAHREANLYLDI
                     LTHDIGNTENVSNLYAELLAGSVEGEAARYAANLKRSIEKSIEILRTVSKIRRIHAGP
                     PDLQPIDLDAVVRAEIAHFPGIPIRYEGAPRTVLADDLLCEVFTNLIGNAVKHGGPGV
                     AVIIRVEAEEEGLLRVTVADTGRGVPEERKEEIFHCYERKQRGVGEGLGLYLVRILID
                     RYGGRIRVEDRIPGYPGEGAAFS"
     assembly_gap    4480..4579
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4580..>6495
                     /locus_tag="PLV90_07960"
     CDS             <4580..>6495
                     /locus_tag="PLV90_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOF97087.1"
                     /translation="VRTKILLVFLGLSMAALLLAGSLAFVQMDDVSRYALARSTDLGA
                     RAVNDSTAALERNAEESLLRLAQDQAYISTILFEQVNGDLEVMGRYAATVMANPSMVR
                     PRHFYLQDEEPEDRRATSVLFLSPGVDGGRLRDERNAAGMMDDIFIPVSATNRHLAGV
                     YVGTGSGMAMIYPWTTGLDATCDPRLRTWFVQAVETGETVWSEPYVDLIGHGLMVTCS
                     QPVYGPERDWVWVVGADVTVEMINRQIVGTQVGDRGYAMLIDQHGNVISRPGLTSGDM
                     RWDESFITENLLASENPDIVAVAKKMVAGETGIARVGFEDGERFVAYAPVRGVNWSVG
                     VVMPVDEVLAPITTTRASILSASEDTALHIGAQQDRMKAVFTGAFVVLLVVVALLTLA
                     VTRYFTRPIEELQRGSEAIGRGDLEHRVNVATGDEFEDLARSFNRMAADLRGHIEDLR
                     RTTAEKERIEKELEIAKEIQQSILPESAPVLPGFDIAGFNLPAREVGGDFYDYIPVGE
                     GRWGVEIADVSGKGVPAALFMALSRTLVRASASENPDPAGSILEANRYICMDSKTCMF
                     VTLFYGILDTRKGTFTYVNAGHNPPLLFRAGSSTAELLRGKGIALGIFDDIELELVEL
                     RLNAGDTVVFYTDGV"
BASE COUNT         1126 a         1927 c         2059 g         1283 t
ORIGIN      
        1 cggtccacct ccctcttccg gaactcgtag cccgaccgga ggagatcgag gaatatggcc
       61 tggtacaccc cccgctcaca cagctcgagg tggtagcgat gcaccgtcga cttcgtcccg
      121 tactttgccg gtacgtcgct ccaggtacac ccggtggaga ggacatacag gatcccgttg
      181 aagaggtgcc ggggatcccg gcgcggccgg ccgacgtggg gtttccgggg cggaaggtgc
      241 ctccggatga tctcccaggt ttcgtcgtcg atctcccgga atgtcatgcg agcctgccat
      301 atcacccggg gttcttatag ttgtgggatg attccgggcg tttttgcgcc ccaaaaagac
      361 cacgtatccg gggtgttatc gctctccggg agcgagcggg tcgtagttcg tgacgatgat
      421 ctcgcggatc tcgccccgtc cgtctccgtt gcagttgatc atcctccgtg ccggaacccg
      481 gtcgatgcgg tatccggcat agaggtcgtc aaagaattcg tcgcacgggt cgacgttctt
      541 tggatccgag ttgctcagca tcagcctggc gccccgggcg tcgcatcgcg cgtaatatgc
      601 cgcaaggcgt ctctgctcct cgtcggtaaa cccttctttt gcatactgcg tgaatgttga
      661 cgtccggttc aggggccggt atgggggatc gagatagacg aaggtctcct ccgatacata
      721 ttgttccgac tgtgcgaagt cgccgtgatg gatggtggtg ttccggagca gttcggcatc
      781 ggcccggagc acatcctcgt tcaggatcgt cgggtttcgg catctgccga acgggacgtt
      841 gaacccgccc tgcaagttga cccggtagag accgttgaaa cacgtcctgt tgaggaagat
      901 gagttgagcc gcccgctcta tccagccctc gccgtactgc ccgaaatcga tggtctcttt
      961 cgcgcggttg aacgtatccc gcatggcgta gtagaaatcc ttccggccga gatcatcctt
     1021 tgtgaggtag tcgtccgata tgccggcaag atagtcgatg agagcgcaga cgtcgctctt
     1081 caccacgctg taggtgagga tcagttcttc gttgatgtcg aatatgtggc actctctgaa
     1141 cctgaaggtg ctgttgaagt ggaagtatac cgctccacca cctacgaagg gttcgacaaa
     1201 gaccgggagg gttccctcgc tgagctcccg gggaattctc ctggtgaacg catcgagcag
     1261 ttgcgccttt ccgcctgccc acttgaggaa cggtcgtgca gtgtatgttg ccatcgctct
     1321 cttccatccg gcgttatttc ctgaaatgtt gttcccgaaa tgttttagtg ttgccggcct
     1381 ccggcgggga tatctcccga tccgggtctt tgtcggtccg gttccgggtg tcggcaacct
     1441 gcctccccgg cgcatcgccg gatcttttgc cggcagcaag tcccggcatc caccggcacg
     1501 gttgctccgg gactcattct tctcgcgaac gaggggccgg cgtcgtcgcc cccctccctg
     1561 ttctccggac cccctctctc ttcagcatct tctcgatggc ccaccgtccc cggccgagtt
     1621 cctccgccag ggtatcgacg gccttcttct tcgtcgcgcc gccctcaata agccgggcga
     1681 accgggagac cagggcctcg atctcatcct ccgtccaggg ccggccccgg tttttcggtc
     1741 ccgaggccag cggagttctc aacgacgaca gcagacatcc ctgcccgatg ccccgcaact
     1801 ctttgacttt caggtcgaag acaggtcctg agagctcaaa cccggtcgcc cgacgattga
     1861 ggccgacggc cgccctggcg gtcgagaatc cgccaaggaa catatcgcag acgaggtcgc
     1921 cctcgttgct gctgtactgc agcatcttta cgagcagttc cgccggcaat tcgttcttgt
     1981 tcttcgcctt cccgggtttg tactcgcggt tgacgaccca gacgtcttca cggtcgcggt
     2041 agttgggcga cccgttatcc ggtgccttct ccccggttcc ataccgcgac tcaaggttga
     2101 acgtcctctt gccgcccggc ttttcgtaga agaggatgtg gtagtgggag gagacgtact
     2161 tccggctggt gtagacgccg aagttgtact tccatatgat gtggttgacc tcgcgaagct
     2221 ctgtctgccg gagggcgtgg agtatgtggt agaggttcgt gtagcccgat acgatgtaga
     2281 tcgatccccc gggccggagt atccgttcgg cctcccggat ccactgccgg ctgaactccc
     2341 cgtacccggc tgcgggaacc tcgacgtagc cctcgacgac gaacgcctcg tcccggttgt
     2401 agtgccggtg cagccggtcg ccgtcgatgc cgtacggggg gtcggtgacg atcaggtcga
     2461 tcgtaccgtc ggggatgtgc tcccgcgccc cgcggatgca gtcgccgttg tagaagatgt
     2521 tgccgttgat ctcttcggac ctcatacgct ctccgcccga atcctgcagg tatcgggctg
     2581 tggtgtacac cagatgggtt gcgcgagaat aagattgtgg cttttccagg tctcacggcg
     2641 gtacgtgcac cggtcacggg gcccggggcg atggttgagt gcgaggagtg ggatcgggcg
     2701 atctctgccg actcccggcg ggaccgacca gcagaggaga ggtgccggcc cggcactccc
     2761 cgttgcggtc ggaagccccg ctcccgcccc cttcacctgc cggaggtagt attatattag
     2821 acgggtgaat ccagctaaga gggagggacg aagcgccccg ggattttaga tgacggcagg
     2881 ttccgatgtg acggagactt catcccgcac gggagcggga gagagggagc agaagacctc
     2941 cgcgatgggg gaggggccag ttcacgagtg tattgccgcg atcctggctg aaaacgaggc
     3001 gctccgacgg gaaaacgctc tcctgcaatc catgaaaaat gccctggagg agggcgagat
     3061 gcgataccgg cggctttttg aggatgccct taccggggac ttcatcacag ccccggatgg
     3121 ccggataatc gcctgtaacc cggcgtttgc cagaatgttc gggttttcct ccgtagaggg
     3181 ggcactctgc accaatgtcc gggatctcta tgaagacccg ggggaccggg accgccttct
     3241 cgcgcagctc cggagaggag gcagggtggt gaacgagggc cggacgcgga agcgccggga
     3301 cggcacccgg atccatgtcg tggagaacgt ggtcgggcgt cttggtgcgg acggcgaact
     3361 catcgagatc cagggttaca cctatgatga ttctgagcgc aaacgggtgg aagatgccct
     3421 ccgggagagc gaagagcggt tccgggcggt cctggaaaac tccctggatt cggcctaccg
     3481 gcgcaacctg caggccgact gctacgacta catgagcccg gcgatcgagg agattcttgg
     3541 ctttacgccg gaggagatgg ccgttatgag cctcgaggag gtcatggacc gcattcatcc
     3601 cgatgatcgt tcgctggtta aagcggaact caacaccgct gctgcttcgg gcagagggct
     3661 cctcgtgtac cggttcctgg ccaaaggggg tgaataccgc tggctcgaag atcacttcac
     3721 ggtgatatcc gattcggctg gccggccgct cttccgcggc ggcatcgtcc gtgatatcac
     3781 cgggcgcagg cgggctgaag aggctatcgc gcggagtgcc gcggagcttg cccgggttca
     3841 ccgggatctc gagtccgctc accgggaggc gaacctctac ctcgacatcc tgacgcacga
     3901 tatcgggaac accgagaacg tctcgaacct ttatgccgaa ctgctcgccg gttccgtgga
     3961 aggggaggct gccaggtatg cggcgaacct gaaacggagc atagaaaaga gcatcgagat
     4021 cctcagaacc gtctcgaaga tccggcgaat tcacgcagga ccacccgatc tccagccgat
     4081 cgatctcgat gcggtcgtca gggcggagat cgcccacttt cccggtatcc ctatcaggta
     4141 cgagggcgct ccccgaacgg tccttgcgga cgacctcctc tgcgaagtct tcacgaacct
     4201 gatcggcaac gccgtaaagc acgggggccc cggtgtcgcc gtgatcatcc gtgtcgaggc
     4261 ggaggaagaa ggtcttctcc gggtgacggt tgcggatacc ggccgaggtg tcccggaaga
     4321 gcggaaggag gagatcttcc actgctacga gaggaagcag cggggcgtgg gcgaggggct
     4381 tggtctctat ctcgtgcgga ttctcatcga ccgctacggc ggcaggatcc gggtcgaaga
     4441 tcgcataccg ggctatcccg gggagggagc ggcgttctcn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnng tccggaccaa gatccttctt gtattcctgg gcctttccat
     4621 ggccgctctc ctccttgcgg gatctcttgc cttcgtgcag atggatgatg tcagccggta
     4681 cgcccttgcg cggagcaccg atctcggggc gcgtgcggtg aacgacagca ccgccgcgct
     4741 tgagcgcaac gccgaagagt cgcttctccg gcttgcgcag gatcaggcct acatcagcac
     4801 catcctcttt gaacaggtga acggggatct cgaggtcatg gggcgctacg ccgcaaccgt
     4861 catggcaaac ccgtccatgg tccggccgcg ccacttctac ctgcaggacg aagaaccgga
     4921 ggatcggcgt gcaacctccg tcctcttcct ctcgcccggg gtcgacgggg gccgtcttcg
     4981 ggatgagcgc aacgctgcag ggatgatgga cgacatattc atccccgtct ccgcaacgaa
     5041 ccggcacctt gccggcgtct acgtcggtac cggttccggg atggcgatga tctacccctg
     5101 gacgaccgga cttgatgcta cgtgcgatcc ccgtctcagg acctggttcg tacaggcggt
     5161 cgagacggga gagacggtct ggtccgagcc gtacgtcgac ctcatcgggc acggcctgat
     5221 ggtgacctgt tcgcaaccgg tctacgggcc cgagagagac tgggtctggg tggttggtgc
     5281 ggacgtcacg gtcgagatga tcaaccggca gatcgtcggc acccaggtgg gcgaccgggg
     5341 ttatgccatg ctgatcgacc agcacggaaa tgtcatcagc cggcccggcc tcacctcggg
     5401 ggatatgcgg tgggacgagt cgttcatcac cgagaacctg ctcgcgagcg agaacccgga
     5461 tatcgtcgcg gttgctaaaa agatggttgc gggggagacg gggatcgccc gggtcgggtt
     5521 tgaggacggc gaacggtttg tcgcgtatgc tcccgtccgg ggcgtgaact ggagcgtcgg
     5581 ggtggtgatg ccggtcgacg aggtgcttgc gccgataacg acgacccggg caagtatcct
     5641 ctcggcgtcg gaggacaccg ccctgcacat cggcgcccag caggaccgga tgaaagccgt
     5701 ctttaccggg gcgtttgtcg tcctgctcgt cgtcgtcgcc ctcctgaccc ttgccgtcac
     5761 ccggtacttc acccggccca tcgaagagct gcagaggggg tcggaggcca tagggcgggg
     5821 cgatctcgag caccgggtga atgtggcgac cggggacgag ttcgaagacc ttgcccgctc
     5881 gttcaaccga atggcggccg atcttcgcgg gcatatcgag gatctccggc ggacgactgc
     5941 ggagaaggag cggatagaaa aggagcttga gatcgcaaag gagatccagc agagcatcct
     6001 cccggagtcc gcgccggtgc tccccgggtt cgatatcgcg ggcttcaacc tgcccgcacg
     6061 ggaggtgggc ggggacttct acgactacat ccctgtcggc gagggtcgct ggggtgtcga
     6121 gatcgccgac gtctcgggga agggcgttcc ggcggccctc ttcatggcgc tctcccgcac
     6181 cctcgtccgg gcgagcgcct cggagaaccc ggatcccgcc gggtcgatcc ttgaagccaa
     6241 ccgctacata tgcatggact caaagacctg catgttcgtc acgctcttct acggtatact
     6301 cgatacccgg aaaggaacct tcacctacgt gaacgcgggc cacaatcctc cgctcctctt
     6361 ccgggcaggg tcgagcacgg cggagctcct ccgggggaag gggattgctc tcggtatatt
     6421 cgacgatatc gagctcgaac tcgtggagct ccggttgaat gccggcgata cggtggtctt
     6481 ctacaccgac ggggt
//
LOCUS       DAOSII010000103         6404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_10972_length_6404_cov_51.535202, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000103 DAOSII010000000
VERSION     DAOSII010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10972_length_6404_cov_51.535202"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLV90_07965"
     CDS             complement(<1..258)
                     /locus_tag="PLV90_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HOF97088.1"
                     /translation="MMEEILAILLAVAIAATVYYFMKKTLTLVINAVAGLATLLLLNY
                     FDVLAWFGAPDIQVNLVTILICALGGLPGALILVLLHLAGIA"
     gene            complement(259..1551)
                     /locus_tag="PLV90_07970"
     CDS             complement(259..1551)
                     /locus_tag="PLV90_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOF97089.1"
                     /translation="MIGDDLIARILREGERRADEVEVFYARGTSVSAEIKKGILGTAE
                     ESEAWSMAIRTVRDGRIGSSSTGDPDRWKECLEAALASGRLATPQQWGGFPKPAEMAG
                     TASSSDPDIVVAIEDAAGMVDALLAGAAEHPVEVVGGSADLARSYIAVANTNGVAYGM
                     DRTVAAVSLEAIREQSTGYEFDASPFRADIDARSVGEEAAALAARSLGGSEVATGRYD
                     VVLSPVAAASILGQVILPALSGRNVKAGRSFLADKVGEQVFDETLSVYDDPFAPGLGS
                     TAWDAEGTPTRRLVFVEQGVLRRFAYDLKTGYRYGEGSTGSAVRSGGDTPGIGFHNLF
                     IDGPRVKEPGDERAVWIHDVVGAHTANPFSGDFSVEISNPFWIEGGDLVEPIRTAMFA
                     GNAFEMLRAIGGLGNDARHVGRLTIPSIRLNNQQIIGK"
     gene            complement(1548..2876)
                     /locus_tag="PLV90_07975"
     CDS             complement(1548..2876)
                     /locus_tag="PLV90_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOF97090.1"
                     /translation="MADVRYYDVRSVRGEVTQIDIDNGVVESAGASFFDKTVIRVLGP
                     RGWGILTRDHVDIDDRRELDGLVAAAARLAAVTKDAISLADAPRGVLAVPPLGEDPRD
                     VDLEEKTRLLAGIEAAARIEGVANTRARYTEGIDAVRFRDSSENEYSFEAVRSGFSVL
                     AIASKNGVMQMGSERDHITTGFNLRDKQGLGRKAGEVAVSLLDAKLPKGGAQRAILDP
                     ELAGVFTHEAVGHASEGDLVREGASVLGGKIGEEIGCRGLVIVDDPSLHEFGFIPVDA
                     EGVAVQRTEIIRDGILRSFLHNRETLAAVGDGIPGHARAEHGAPPIVRMSNTFIENGD
                     ASYDELLAECRDGILLIGSRGGQVDPGRGIFQFNAEYGYIVRGGETTDMVRDVSLSGE
                     ILSTLHNITLIGDDRRMSPGYCGKGGQSVPVSDGAPHLLLEGAVIGGRGE"
     gene            complement(2883..4823)
                     /gene="lonB"
                     /locus_tag="PLV90_07980"
     CDS             complement(2883..4823)
                     /gene="lonB"
                     /locus_tag="PLV90_07980"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease LonB"
                     /protein_id="HOF97091.1"
                     /translation="MDLTTPEEIPKIELTNDELAEADVFAGLELSASSDIDVPPNLID
                     QVIGQEHAVEVIRKAAVQRRHVMMIGTPGTGKSMLAKAMAELLPKEELQDILVYPNSE
                     DNNNPIVRTVPAGRGKQIVNAHKMEARKKIQMRSTLIMLLVFGIIGYAIIASQWLMGI
                     IAAAFVFMALKYSTPREEAMVPKLLVTHDPNTPAPFVDATGSHAGALLGDVRHDPFQS
                     GGLETPSHDRVEGGAIHRAHGGVLFIDEINTLTPHSQQNLLTALQEGTFPITGQSERS
                     SGAMVRTEPVPCRFVMIAAGNLDAIQGMHPALRSRIRGYGYEVYMRDTMEDTPENRKK
                     FLRFIAQEVKNDGKIPHFDRSAMLEVLREARRRSNRKGHLTLKLRDMGGLIRVAGDLA
                     RQEGADITTARHVIAAKSAARSIEDQISDEYIRRSRDYEITVVEGARVGRVNGLAVMG
                     SDSGSVLPVMAEVTPSQGANGSVIATGMLKEIAKESITNVSALIKKFTGKDIRNMDIH
                     IQFIGTYSGVEGDSASVTVATAVISAVENIPVRQDVAMTGSLSVRGDVLPIGGVTYKI
                     EAAAKAGIKRVIIPRSNLEDVLIEDRYRTMVEVVPVEHIEEVLQNALVPENRESFLSK
                     LRKMTVNPTAGIFDPNVGRTLV"
     gene            4905..5762
                     /locus_tag="PLV90_07985"
     CDS             4905..5762
                     /locus_tag="PLV90_07985"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOF97092.1"
                     /translation="MRIISTERSQVLAARVAAKLGVPLVETKFTRFPDGEMYLRCGEL
                     DDETLIVSSTVDNDMFVQTLLLIDAADRSKNTLVAPYLGYSRQDRRFVPGEPISARAV
                     ARALSAGTERAFVVNIHDPGVLQHFGVPAKNITIAPAIGGYIGDLRLKNPLILAPDEG
                     AIEFATGVAAVGGWDCDHLEKTRLSGDEVRIAPKTIDAAGREVVIVDDIISTGGTLAT
                     AACMLREQGAASIHAACVHGVFTSGAYTRLRAAGIASVVSSDTYENASSFISAANAIA
                     TAIRDDAHH"
     gene            5749..>6404
                     /locus_tag="PLV90_07990"
     CDS             5749..>6404
                     /locus_tag="PLV90_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HOF97093.1"
                     /translation="MLTIDGSCLEGGGQIVRLAVALSALAGIPVAVTRIRENRPNPGL
                     APQQIAAVRAVAGVCNAECRGLTVGSREITFAPGDLRRADLRIDPGTAGSITLILQAW
                     LPVALRTGGSITVTGGTEVAWSPTIDYMDRVLAPVLRNAGASIAIDVLERGYYPRGGG
                     RVHVGVESTCPGPITIPEEGECGIVSCSAGLPAHVVERQAAAAREILCRDPGLSCTVT
                     T"
BASE COUNT         1050 a         2127 c         2068 g         1159 t
ORIGIN      
        1 cgcgatcccg gccaggtgga gcagcaccag gatcagggcg ccggggagcc ccccgagggc
       61 gcatatgagg atcgtcacca ggttgacctg gatgtcgggt gccccgaacc aggcgaggac
      121 atcgaagtaa ttcaggagca agagcgtggc gagcccggcg acggcgttga tgacgagcgt
      181 cagggtcttc ttcatgaagt aatagaccgt cgcggctatc gctacggcaa gaaggatggc
      241 aaggatctct tccatcatct atttaccaat gatctgctgg ttatttaacc gtattgatgg
      301 aatcgtcagc cgtccgacat gccttgcgtc attcccgagc ccccctatcg cccggagcat
      361 ctcaaaggcg tttcctgcaa acatagccgt ccggatgggt tcgacgaggt ctccgccctc
      421 gatccagaag gggtttgata tctccacgga gaagtcgccg gagaaggggt ttgcagtatg
      481 ggcgcccacg acgtcgtgga tccagaccgc ccgctcgtcg ccgggctctt tcacccgcgg
      541 cccgtcgatg aagaggttgt gaaaccctat accgggagta tcgcccccgg agcgcacggc
      601 gctcccggtg ctcccctcgc cgtagcggta gccggtcttg agatcgtagg caaaccgccg
      661 gagcaccccc tgctccacga agaccaggcg gcgggtggga gtgccctccg catcccaggc
      721 ggtgctgccg aggccggggg cgaaggggtc gtcgtagacc gagagggtct cgtcgaagac
      781 ctgttcgccg accttatccg caaggaacga ccgaccggcc ttcacgttcc gcccggagag
      841 ggccgggagg atgacctgcc cgaggatgct tgcggcggca accggcgaga ggacgacgtc
      901 gtagcgcccg gtcgcgacct cagaaccccc gagggagcgg gcagcaagcg cggccgcctc
      961 ttctccgacc gaccgggcat cgatatcggc acggaacggg gaggcgtcga actcgtagcc
     1021 ggtggattgc tcccggatcg cctcgaggga gaccgccgcc accgtccggt ccatgccgta
     1081 ggcgacgccg ttcgtgttcg cgaccgcgat atacgaccgg gcgaggtctg cagacccccc
     1141 gacgacctcc acgggatgct ccgccgcacc cgcgaggaga gcatcgacca tcccggccgc
     1201 atcctcgatc gcgacgacga tatccgggtc cgacgacgat gcggtgccgg ccatctcggc
     1261 cggcttcggg aacccgcccc actgctgcgg ggtggcgagg cggccgcttg caagcgccgc
     1321 ctccaggcac tccttccacc ggtcgggatc gccggtggat gaggagccta tccgtccgtc
     1381 cctgacggtc cgtatggcca tgctccaggc ctcggactcc tcggcggtgc cgaggatccc
     1441 cttcttgatc tcggcgctga cgctcgtccc ccgggcatag aagacctcga cctcgtcggc
     1501 ccggcgctcg ccttcccgga gaatcctcgc gatcaggtcg tcgccgatca ttcgccccgc
     1561 cccccgatga ccgcgccctc aagcaggagg tgcggcgcgc cgtcgctgac cgggacgctc
     1621 tgcccgccct tgccgcagta gcccgggctc atccttcgat cgtccccgat gagggttatg
     1681 ttatggagcg tcgagaggat ctcgcccgag agcgagacgt cacggaccat atcggtcgtc
     1741 tcgccgcccc tgacgatata gccgtactcc gcgttgaact ggaagatgcc ccgcccgggg
     1801 tctacctggc cgccccttga gccgatgagg aggatgccgt cccggcactc ggcgaggagc
     1861 tcgtcatagg atgcatcgcc gttctcgatg aacgtgttgc tcatccggac gatcggcggc
     1921 gcaccgtgct ccgcccgggc gtggccgggg atgccgtccc cgaccgccgc gagggtctcg
     1981 cggttgtgga ggaacgatcg aaggatccca tcgcggatga tctcggtccg ctgcactgcg
     2041 acaccctcgg cgtccaccgg gatgaacccg aactcatgga gggagggatc atccacgatc
     2101 acgagcccgc gacagccgat ctcttcgccg atcttccccc cgaggaccga tgcgccttcg
     2161 cggacgaggt cgccctcgct cgcgtggccg accgcctcgt gggtgaagac ccccgcaagc
     2221 tccgggtcga gtatggcccg ctgcgcccca cccttcggga gcttcgcatc gaggagcgag
     2281 accgcgacct cgccggcttt ccggccgagg ccctgcttat cccggaggtt gaacccggtc
     2341 gtgatgtggt cgcgctcgct tcccatctgc atcacgccgt tttttgaggc gatggcaagg
     2401 accgagaatc ccgaccggac cgcttcaaag gagtactcgt tctcgcttga gtcccggaac
     2461 cggacggcgt cgatcccttc ggtgtaccgg gcccgggtgt ttgcaacgcc ctcgatccgt
     2521 gccgctgcct cgatcccggc aagcagccgg gtcttctcct cgaggtcgac gtcccgaggg
     2581 tcttctccga gaggggggac cgcgagaacg ccgcgcggtg cgtcggcaag gctgatagca
     2641 tccttcgtga cggcggcaag gcgtgccgcc gccgcgacga gaccgtcgag ttcgcgcctg
     2701 tcgtcgatat cgacgtggtc gcgggtgagg atgccccagc ctctcggccc gagcacccgg
     2761 atcaccgttt tgtcaaagaa cgaagcgcct gcggactcga ccaccccgtt atcgatatcg
     2821 atctgggtga cctcgcctcg cacgctcctg acatcatagt acctcacgtc tgccatacga
     2881 catcagacca gggtgcggcc gacattcggg tcgaatatgc cggccgtcgg gttgaccgtc
     2941 atcttccgga gcttcgagag gaagctctcg cggttctcgg ggacgagagc gttctggaga
     3001 acctcctcga tgtgttcgac cggtaccacc tcgaccatcg tgcggtagcg gtcctcgatg
     3061 aggacatcct ccaggttcga ccgcggtatg atcacccgct tgataccggc ctttgccgcc
     3121 gcctcgatct tgtaggtcac cccgccgatg gggaggacgt cgcccctgac cgagagcgac
     3181 ccggtcatcg cgacgtcctg gcgcaccggt atgttctcga cggcactgat caccgccgtt
     3241 gccacggtca cggaggccga gtcgccctcc acgccgctgt aggtgccgat gaactggatg
     3301 tggatatcca tatttctgat atccttgccc gtgaacttct tgatcagggc gctgacgttc
     3361 gtgatcgatt ctttcgcgat ctcttttaac ataccggtcg cgatcaccga accgttcgcc
     3421 ccctggctcg gtgtcacctc ggccatcacc gggaggaccg atcctgagtc gctccccatc
     3481 accgcaagcc cgttcacccg gccgacccgg gcgccctcga cgacggtgat ctcgtagtcg
     3541 cggctccgcc gtatgtactc gtcggatatc tgatcctcga tcgagcgggc ggccgacttc
     3601 gcggcgatga cgtgccgtgc cgtggtgata tcggcacctt cctgtcgggc gaggtccccc
     3661 gccacccgga tgagcccgcc catatcgcgg agtttcaggg tcaggtggcc cttccggttc
     3721 gagcggcggc gggcctccct gagcacctcg agcatggcgc tccggtcgaa gtgggggatc
     3781 ttcccgtcgt tcttgacctc ctgggcgatg aatcggagga acttcttccg gttctccggc
     3841 gtatcctcca tcgtgtcccg catgtagacc tcgtagccgt agccgcggat acgcgaccgg
     3901 agcgccgggt gcatcccctg gatggcatcg aggtttccgg ccgcgatcat gacaaatcgg
     3961 cagggaaccg gttcggtccg gaccatcgca ccgcttgaac gctcgctctg gccggtgatc
     4021 gggaaagtgc cctcctggag agcggtcagc aggttctgct gggagtgcgg cgtgagggtg
     4081 ttgatctcgt cgatgaagag caccccgccg tgcgcccggt gaatggcccc accctcgaca
     4141 cggtcgtggg acggcgtctc aaggccgccg ctctggaacg ggtcgtgccg cacgtcgccg
     4201 agcagggcac ccgcgtgcga gccggtggcg tccacgaagg gcgcgggggt gttcgggtcg
     4261 tgggtgacca ggagcttcgg aaccatcgcc tcctcgcggg gcgtcgagta cttcagcgcc
     4321 atgaagacga acgcggcggc gatgattccc ataagccact gggaggcaat gatggcgtag
     4381 ccgatgatac cgaagacaag gagcatgata agagtgctcc gcatctggat cttctttctc
     4441 gcctccatct tgtgggcgtt gacaatctgc ttgccgcgac cggcgggaac ggtccggacg
     4501 atcgggttat tgttgtcctc ggagttcgga tagaccagga tatcctgcag ttcctctttc
     4561 gggaggagct ccgccatcgc tttggccagc atcgatttgc cggtcccggg ggtgccgatc
     4621 atcatcacgt gcctgcgctg gaccgctgcc ttccggatca cctcgacggc atgttcctga
     4681 ccgatgacct gatcgatgag attcgggggt acatcgatat ctgacgatgc gctcagctca
     4741 aggccggcaa agacgtcggc ttccgccagc tcgtcgtttg tgagctcgat cttcgggatt
     4801 tcttctggag tcgtcaaatc catcgtcggg tttacctctt tgatgcactg atacttatat
     4861 aatttattca tcctcgttca aatactttcc agcaaaggtg ttacatgagg ataatcagca
     4921 cggaacggtc ccaggtcctc gcggcccgcg tcgccgcgaa gctcggggta ccgctggttg
     4981 agacgaaatt cacccgattt cccgacgggg agatgtatct gcggtgcggg gaactggacg
     5041 acgagaccct gatcgtcagc agcaccgtgg acaacgatat gtttgtgcag acgctccttc
     5101 tgatcgatgc cgccgaccga tcgaagaaca ccctggtggc cccgtacctc ggctactccc
     5161 gccaggacag gcggtttgtc ccgggtgagc cgatcagcgc cagggcggtc gcacgagctc
     5221 tctctgccgg caccgagcgg gcgttcgtgg tcaacatcca cgaccccggc gtgctgcagc
     5281 acttcggcgt cccggcaaag aacatcacca tcgctccggc catcgggggg tatatcggcg
     5341 acctgcgcct gaaaaacccc ctcatccttg ccccggacga gggagcgatc gagttcgcga
     5401 ccggcgttgc ggcggtcggc gggtgggact gcgaccacct tgagaagacc cggctctcgg
     5461 gcgacgaggt ccggattgcc ccaaagacca tcgatgccgc cgggcgcgag gtcgtgatcg
     5521 tggatgatat catatccacc ggcggaacgc ttgcgaccgc ggcctgcatg ctccgggaac
     5581 agggagcggc atccatccac gcggcctgcg tccacggggt gtttacgagc ggtgcctaca
     5641 cgcgcctgcg ggcggcgggg atcgcctcgg tcgtctcaag cgacacctac gagaacgcct
     5701 caagcttcat atcggccgcg aacgccatcg ccaccgcgat cagagacgat gctcaccatt
     5761 gatgggtcct gcctcgaggg gggcgggcag atcgttcgcc tggcggtcgc cctctcggcg
     5821 cttgccggga taccggtcgc cgtgacccgg atacgggaga accggccgaa ccccggtctt
     5881 gctccccagc agatcgccgc ggtccgggcg gtcgcggggg tctgcaacgc cgaatgccgg
     5941 gggcttacag tcgggagccg cgagatcacg ttcgctccgg gcgacctccg gcgggccgac
     6001 ctccggatcg atccggggac tgccgggagc atcaccctca tcctccaggc gtggctcccg
     6061 gtcgccctcc gcaccggggg gagcataacg gtcacggggg ggaccgaggt ggcctggagc
     6121 ccgacgatcg attacatgga ccgcgtcctc gcaccggtgc tccgcaacgc cggggcctcg
     6181 atcgcgatcg acgtgctcga gcggggttac tacccgcggg gaggggggcg ggtgcacgtc
     6241 ggggtggaat cgacctgccc gggcccgatc acgatcccgg aagaggggga gtgcggtatc
     6301 gtctcctgct cggcaggcct ccccgcacac gtcgtggagc ggcaggcggc cgcggcccgg
     6361 gagatcctct gccgggatcc gggcctctcc tgcacggtga ccac
//
LOCUS       DAOSII010000104         6198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_11454_length_6198_cov_41.300179, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000104 DAOSII010000000
VERSION     DAOSII010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6198
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11454_length_6198_cov_41.300179"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            394..552
                     /locus_tag="PLV90_07995"
     CDS             394..552
                     /locus_tag="PLV90_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97094.1"
                     /translation="MKRINAKGEVVQQTSIYTRENAYLLAKEASIPMSEAAEQGVIAA
                     LRARGVEV"
     gene            562..876
                     /locus_tag="PLV90_08000"
     CDS             562..876
                     /locus_tag="PLV90_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97095.1"
                     /translation="MTKYEIVFKESAKKDLKVIERDDGEYVSKILEKIEFCLGEHLFD
                     RTGQCSKKKMEGKEKAHRLHVQKRYTIFYTVERTKEKTFVEVHLIMSYAATHKRYKRI
                     DL"
     gene            complement(871..1056)
                     /locus_tag="PLV90_08005"
     CDS             complement(871..1056)
                     /locus_tag="PLV90_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97096.1"
                     /translation="MPTKTVVERKKATPKAAPKKALTPAEQAERQASIDRLDADIRKS
                     KMGKSYTLAEIKKEFGL"
     gene            complement(1457..3034)
                     /locus_tag="PLV90_08010"
     CDS             complement(1457..3034)
                     /locus_tag="PLV90_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97097.1"
                     /translation="MSTMRPLRIAILLFCLILTVPAATAGNLTILQPSANESTFAEMR
                     DFYVYGVFPEPLEHPGNIRLVLTDESGVIVRTVMSCVNETGVTPEAAVEMSLVPNRWG
                     GLLAPDVITEPGGIANGSNKVLVTGTYYLALVQGGVTRGMGGYTDVSGGTLAPITRDL
                     TAGTYTLVVEGLDGDLAGERVEKELVFGTTHASLGTFRPDANRENVIGWARERVPPLR
                     VYIDWFPGYFRSGDAGYEIPDRWQANNGIEVANDCTGTTVDTIASARNDCLIYNVGST
                     SATLQVEIAAILRNGLVDSDRTTFTHYDIGEPSMAWVDRATGAAESMIGRQVAYPAGT
                     RLVYTRAEITDEPVRTNYFNTVANDSSKTVDTTPMQVTVVQGENLTFYGVTRPIETTL
                     TGAGSAYRYTPDDRIATYIYENETLGRFTFTGCLEREFEGGYIGAAGYEFGHAFTETA
                     AMPAGTWLLAVSAYDTTGTPVEMTNRTIELEVAAPPALPTATLVAAAAPAPTRTSGFW
                     SVPALVALLAAVAARRR"
     gene            3386..3595
                     /locus_tag="PLV90_08015"
     CDS             3386..3595
                     /locus_tag="PLV90_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOF97098.1"
                     /translation="MKLSARNQLPGKVTKIDTGVVTAEVTIALDGGGEITSVITKKAV
                     ENLGLTVGKKVYAVVKSTEVMVAID"
     gene            3784..4101
                     /locus_tag="PLV90_08020"
     CDS             3784..4101
                     /locus_tag="PLV90_08020"
                     /inference="COORDINATES: protein motif:HMM:NF022420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2769 domain-containing protein"
                     /protein_id="HOF97099.1"
                     /translation="MDTFEQFAQKLMKMPLIARIDALKAERAKCICMNCPTYTECAEK
                     LDQGFFCFTGMSIICIDREVECICPTCQVPKDTGLQHTAFYCTRGDEKARRYDRSLAE
                     KTK"
     gene            complement(4806..5252)
                     /locus_tag="PLV90_08025"
     CDS             complement(4806..5252)
                     /locus_tag="PLV90_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HOF97100.1"
                     /translation="MAECAEEKGARMDRAIYPSDIVYVGSTEKRGRGVFARRDISAGE
                     LIEVCPVIAMDETDGQRLLDRTGLFNYYFAWGRHEESAAVALGYGSLYNHSYHANADH
                     VRDVAGGEIRIYACRAIRGGEEITINYGGLPDCPDPVWFDVVEEER"
     gene            5595..>6198
                     /locus_tag="PLV90_08030"
     CDS             5595..>6198
                     /locus_tag="PLV90_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF97101.1"
                     /translation="MGDVIVARDLEKRFPDLTAVDRINFRVREGEVFGFLGPNGAGKT
                     TTMKMIQCISPKTGGTLEVLGMDVDTRQREIKGRLGVVPQENNLDPDFSAYQNLLIYA
                     RYFGIPKREAERRAEELLTFMQLEEKRDVLIDRLSGGMRRRLIVARALINAPELLILD
                     EPTVGLDPQARHLIWEKLRSLRAEGNTLVLTTHYLDEAERL"
BASE COUNT         1280 a         1892 c         1803 g         1223 t
ORIGIN      
        1 tatgacgtcg cccttacacg gcgaagatcc ccggttcgaa tccgggcagg cccatttctc
       61 tccttccttt tttcgaaaca agggctaaac tgtgctgtgt tatgtttctg agcccggaga
      121 ttgctttatc gagcaatcaa gagacgttaa tggcgcattt cattggacac tcgggattct
      181 cagggtcgat agtgacctct aaccgcttcc tgacatgttc tggtaacggg cattctgccg
      241 ttttactcca tctactcatt tctttttctc ctgcccaggc ataacctcag gatgctttac
      301 cctctgtgcg ggcggaccat aaagagcggg tgccaaaagg gcggtttgtt tgacaccctc
      361 tctttaagag gccacaatag ccacgtactg actatgaagc gcatcaacgc aaagggggag
      421 gtcgttcagc agacctccat ctatacccgt gagaacgcct acctccttgc gaaggaagcg
      481 agcatcccta tgtctgaagc tgcagaacag ggtgtgatcg ccgcgctgcg tgcgcggggc
      541 gtcgaggtct gagggaggcg catgacgaag tacgagatcg tattcaaaga gagcgcaaag
      601 aaagacctta aggtaatcga gcgcgatgat ggtgagtatg tgtcaaaaat cctcgaaaaa
      661 atagagttct gcctgggcga gcaccttttc gatcgaaccg gccaatgcag taagaagaag
      721 atggaaggaa aggagaaggc ccatcgattg catgttcaga agagatacac gatattttac
      781 acggtagaga gaacaaagga aaaaacgttt gtcgaagttc acctgattat gagttacgcg
      841 gcgacgcaca agaggtataa acgcatcgat ttatagcccg aattcctttt ttatctcggc
      901 cagagtataa ctcttaccca ttttgctctt tcgtatatcc gcatctaatc tatcgatgct
      961 ggcctgccgt tcagcctgct cggccggggt aagggccttt ttaggcgcgg ccttcggggt
     1021 tgccttcttc cgctcgacta ccgtcttcgt cggcatacag atgatgtcta tatctggaaa
     1081 gatatagttt tggggagaat cacgttcgcg ctgcgcgcct attgagggcc ggctattctt
     1141 gcttatagtc actcccgtaa cgatttggaa gcatgctttg gactcaagaa ttcacccgtt
     1201 catttcgcct gataactgct tttcgccgct cctttcttaa acacctctgt gatcgtggcc
     1261 atcagatgct cacggtcggc gataaacgtc gctgctacct ctcgtttgat cgttcaccat
     1321 acctgctctc tcgtttgatc gtcggcgtac aggcatgggt tctttcctgc acccggccgc
     1381 actgcagccc cttatgccct ccattgcgta tcggggccgc aggggagttc cgggtgaggc
     1441 ggctgcctgt ccggcctcac ctgcggcgag ccgcaaccgc cgccagaagc gccacgagcg
     1501 ccgggacaga ccagaacccg gacgtccggg tcggggcagg cgccgccgct gcaacgagcg
     1561 tcgccgtggg cagagcgggc ggtgccgcta cctccagctc gatcgtccga ttcgtcatct
     1621 ccacaggggt gccggtcgtg tcgtaggcgc tcaccgcgag cagccaggtt cctgccggca
     1681 tcgccgcggt ctctgtgaac gcatgcccga actcgtagcc ggcagcaccg atgtagccgc
     1741 cctcgaactc gcgttccagg cacccggtga acgtgaaccg ccccagcgtc tcgttttcgt
     1801 agatatacgt tgcaatccgg tcgtcggggg tgtaccgata ggcgctcccg gcacccgtca
     1861 gcgtcgtttc aatcggccgt gtcaccccgt agaacgttaa attctctccc tgcacgaccg
     1921 tcacctgcat cggcgtcgta tcgacggtct tgctgctatc gtttgccacc gtgttgaagt
     1981 aattcgtgcg caccggttca tccgtgatct ccgcgcgggt gtagaccagc cgggtgccgg
     2041 cgggatacgc cacctgcctt ccgatcatcg attcggccgc gccggttgcc cggtcgaccc
     2101 atgccatcga cggttcgccg atgtcgtagt gcgtgaacgt cgtgcgatcg gagtccacaa
     2161 ggccgttcct gagtatcgcg gcaatctcca cctgcagggt cgcactggtg ctgccgacgt
     2221 tgtagatgag gcagtcgttt cgtgcagagg cgatcgtgtc gaccgttgtt cccgtacaat
     2281 cgtttgctac ctcgataccg ttgttcgcct gccaccggtc cgggatctcg tatcctgcgt
     2341 cgccggatcg gaagtatccc ggaaaccagt cgatatagac acggagcggc ggcaccctct
     2401 cccgagccca gccgatgacg ttctccctgt tcgcatcggg gcggaacgtg ccaagagagg
     2461 catgggtcgt gccgaagacg agctcttttt ccaccctctc cccggcgaga tccccgtcca
     2521 gcccctcaac gaccagcgtg taggtccctg cggtcaggtc ccgcgtgatc ggcgcgagcg
     2581 tcccgccgct cacatcggtg tagcccccca tcccccgggt gacaccgccc tgcaccaggg
     2641 caaggtagta cgtcccggtc accagcacct tgttgctgcc gttcgcgatg cccccgggtt
     2701 cggtgatgac gtcgggagca agcagccctc cccacctgtt cggaacaagg ctcatctcca
     2761 ccgcggcctc cggcgtgaca ccggtctcgt tgacgcagct catcaccgtc cggacgatca
     2821 cgccgctctc atccgtgagg acgaggcgga tgtttcccgg gtgttccagc ggttctggaa
     2881 agaccccgta gacatagaaa tcccgcatct cggcaaacgt gctctcgttc gcggaaggct
     2941 gcagaatggt gaggtttccg gcggtggcgg caggaacggt caggatcaaa cagaagagaa
     3001 ggatagcgat gcgcagaggt ctcatcgtgc tcatatcagg ataaagatca cctgcaaggt
     3061 taaaggtgcc ggaacgagcg ggaaagagag atcggggact gacggaatag gcgggggtcc
     3121 agtcgccggg acagcgatcc cctcgtttgt gcgcgcatgg cttctgggtc agccggcccg
     3181 gccggggcga acccgactcg aacttcagcc ccggcttatc cgcagcaaga gcgatatcga
     3241 gccccggtat agccggcact cattcctgct gtcgatttgc ctgttcgccc acgggatatc
     3301 cgctttctat ggcacacaac cgcacggggg ttccggagat cgcaacgtat atcactgagg
     3361 gcgtagagca gcatcatggt gcatcatgaa gttaagtgca agaaaccagc tcccgggaaa
     3421 agtaacgaag atcgatacgg gcgtggtcac cgccgaggtc accatcgcac tggacggcgg
     3481 cggtgagatc acctcggtca tcaccaagaa ggccgtggag aacctcgggc tcactgtcgg
     3541 caagaaggtc tacgccgtgg tcaagtcgac agaggtcatg gtcgccatcg actgaccggc
     3601 cggaacccat cagggtcgcc tccgtaccga gatctgccct tccggagcaa ccggaagggc
     3661 gccagactgt tttacctcat ggtatgctcc acgagaagca ccctgccgcg acccctgtat
     3721 tcacgcaggc ggtcttttat acctgtatgg aacaggtggc gcgaagatca ggagagtgac
     3781 gacgtggaca ctttcgaaca atttgcacag aaactgatga agatgcccct tatcgcgcgc
     3841 atagatgccc tcaaagccga gcgagcgaag tgcatctgca tgaactgccc gacctacacc
     3901 gaatgtgcgg agaaactgga ccagggcttc ttctgtttca ccggcatgag catcatctgc
     3961 atcgaccgag aggtcgagtg catctgccct acgtgccagg ttccgaagga taccggactt
     4021 cagcatacgg cattctactg cacgcggggc gatgagaagg cgcgaaggta tgaccggtcc
     4081 ctggccgaaa agacaaagta gaatccccgg ccgtcacgga gaggcaacaa ccctccgggc
     4141 ggcaaagggg acctgagaac ggggtacgcg ggcggcgctc gagtgcctcg taccccggga
     4201 tgccctccct cccggagcgg ggcgagggcc accgccccac cccggatggc gccctttatt
     4261 accagttgcg gcgggcgtta tgcactgtcg gccacctggt agaggacccc cggcccctgc
     4321 aggccgatcc cggcgtctcc tgatcacgct cccgcccgca tgtatccgct tcccctatac
     4381 cccacctaga taagtcgaaa atgcccgtga attccccaaa atgcctcaaa tcatggaact
     4441 cttccgggcg acccatacgg cttgagaaac gatcacaggg accggcagcc ggtatggagg
     4501 cgggagcaga attcaatgat cctgagttat ttcgctaaac cccttgattt ccactggaaa
     4561 ttctggcaca gccacctggg tggcccggag gggccggggc gccgtctcat cccgtactat
     4621 cggcagacgc cacacgtcat ccggggtata aacgcggtcg ggaaataccc ggaaagcaga
     4681 tagccggggc ccccggcatc gtgccgatag ggatgtcaac caccccgccg gggcaccgca
     4741 ggcaccatag ccggatggag acgaccggcg tgagttggag agaaatctgc gggaaagagg
     4801 aggagttatc gctcctcttc caccacatcg aaccagacgg ggtcggggca gtcgggcagg
     4861 ccaccgtagt tgatggtgat ctcttcgccc ccccggattg cccggcaggc gtagatgcgg
     4921 atctcgccgc cggcaacgtc gcggacgtga tccgcattcg cgtggtagga gtggttgtag
     4981 agcgacccgt agcccagcgc cacggctgcc gactcttcgt gccgccccca ggcgaagtag
     5041 tagttaaaga ggcccgtccg atcgaggagc ctctgcccgt ccgtctcatc catcgcgatc
     5101 accgggcaga cctcgatcaa ctcaccggcc gagatatccc tgcgggcaaa tactccccgg
     5161 ccccgcttct ccgtcgaacc gacgtatacg atatcagaag ggtatatggc cctgtccatc
     5221 ctcgcacctt tctcttcagc acattctgcc atggtatcac tggagtcccg cacctccccc
     5281 ggggcactca cccctgtgaa ccgcctcgct ccggcgcgcc ggagttgcgt ggaggggtga
     5341 tagacggtgt gctccccggg ggaaccgcag aaacgtatga attacagtgg ttggctctac
     5401 ctgctcaaaa agaatgcggg atcgaatacg gatagaccga accgggaccc ggttttcctg
     5461 caaaaatccg gcatatagcc ggccggggat ccgtcgcgcg ggatctctcc cgaccaccgg
     5521 agagggaggg ggacgagcaa gcgaaaggat cttctatctg cacgacccca ctcactcatc
     5581 aggaggagca cataatcggc gacgtcatcg tagcgcgcga tctcgagaaa cggtttccgg
     5641 atctcaccgc agtcgaccgc atcaacttcc gggtcaggga aggagaagtc tttgggtttc
     5701 tcgggccgaa cggggcgggg aagacgacga ccatgaagat gatccagtgc atctccccta
     5761 agaccggcgg cacccttgag gtcctcggca tggatgtgga tacccgccag cgggagatca
     5821 agggccgcct cggcgtggtg ccgcaggaga acaacctcga cccggacttc tcggcctacc
     5881 aaaacctgct gatctacgcc cgttacttcg gcataccgaa acgggaggcg gaacggcggg
     5941 cagaagagct ccttacgttc atgcaactcg aggagaagcg ggacgtgctg atcgatagac
     6001 tctcgggcgg catgagacgg cggttgatcg tcgcccgggc cctcatcaat gcacccgaac
     6061 tgctcatact cgacgaaccc accgtcgggc ttgacccgca ggcccgacac ctgatctggg
     6121 agaaactcag gagtctccgg gcggaaggaa acaccctcgt cctcaccacg cactacctgg
     6181 acgaagcgga acgtctct
//
LOCUS       DAOSII010000105         6164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_11527_length_6164_cov_53.126044, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000105 DAOSII010000000
VERSION     DAOSII010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11527_length_6164_cov_53.126044"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PLV90_08035"
     CDS             <1..531
                     /locus_tag="PLV90_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97102.1"
                     /translation="RTKEPRNLLDGERAVWEFSVTTHVGRLMLENLTLPEERLITDLT
                     SYPDAFAFRAYALAGTQEIPSMGLSDPVIAIRSAGSAAAESSLPPLYTLVGVQDSLLD
                     PSETMVDAWRRGIDYDAITAGAGEHLGTLASLGKEEVAAIYTAEGRAGAEAAAGTSPS
                     IIDLIVEFFRSIFGEV"
     gene            complement(544..1575)
                     /locus_tag="PLV90_08040"
     CDS             complement(544..1575)
                     /locus_tag="PLV90_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1786 domain-containing protein"
                     /protein_id="HOF97103.1"
                     /translation="MIDLTTPLLAIDVGRGTQDILVYEPGRSIENSIKLVLPSPTVVT
                     ADRIRRATRAGRPIFLEGFLMGGGANTGAVRQHLAAGLPVYATPDAAGTLHDDPERVR
                     SMGVAIEPNPPEDAVVVHTTDYMESELRAALSLFGIAYPENVAVAVQDHGYSPHRSNR
                     IHRFELVRERLDAGDWDLLSFVSDPPLAAMTRMQAVRRQAPGALVTDTGPVALIGALC
                     DPEVRRMADKGVILVNAGNGHTLCFALKGREIYGIFEHHTGALTPAKLRHYIRRLADG
                     TLTSEEIFDDGGHGAAVRKPLPTDALAVTGPNRLRLLPEACQAAPFGDMMLSGCFGLM
                     HLWKQFREG"
     gene            complement(1660..>4818)
                     /gene="nrdD"
                     /locus_tag="PLV90_08045"
     CDS             complement(1660..>4818)
                     /gene="nrdD"
                     /locus_tag="PLV90_08045"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOF97104.1"
                     /translation="EIVVDVSPAEPTGEYVYDLFIDGDGTEESHTFYASDGLLIHNCQ
                     DWDLRYFFYYGLMPDGNGTKASVAGPAKRAEVAVLHAVKALGSAQTNFAGGQGYYNFL
                     TFMAPFFEGMEYGEIKQLMQMFVYEMTQMMVARGGQVVFSSVQLSPGVPTLWRDKPCV
                     YRGKIWDGNQAPLRTYGEFEREVRLLFKALMEVMLEGDYWGKPFSFPKPEISIEPDFL
                     IENEAYNREHPDLPTYHDLYLMTFELASKFGTPYYDNQIPAYRGAGEGISCYQCLAGD
                     ELVPVADSTGRVVVRSIRSLFDAAAKNGRRVDPFGTELAYYDGKTPSVDFTTLEASLR
                     PFRGVMRRRYTGDLLRITLESGRQITVTPDHPVYVLNGGGFARETAGDLEAGDYLPVL
                     TAEGLGEGSVTDIDLAEVLTKAGHAGAIRVTEDAVTIPGTRHPGLPRTLPVSPELAVF
                     LGYYLAAGSSDHAGRHYTVRLAFGRHDADSAADAAACIRAALGYEPQIQKSTTGIDVV
                     VRSKLIFLLVDALGCGSSAETRSVPDLLFNLDRILAGDYLGAAFLATGKRTIGRRARS
                     IRLKTASEDAARKVVWLAGQLGIQMSYAEREHGIHRPGAPQTTYTCSITAQDQIERFS
                     AATGFPAEMPLGRERGGAFTRLPEGERARGYTALACASKYSAGGVEGVQVSLFLPPIT
                     QPAGKGRFAGGDVHPLRVRSVEPVVYDGHVYDLVDVAGTHTFANALGIVTGNCCAYQF
                     SSLADEDDEFEDKLHFREGKHFSMGSWQVMSINCPRAAYKAEGNQERLFAELKALMDT
                     AVGLYRIKRRWMSLIRANGRMPFAMQRPKDPNTGERGAVAVDLEGLVYTIGVVGVNEM
                     VQHFTGHQLHESKEAFRLAVRAMTELEMYARELSQKHNMTIALARTPAETTGQRFAVA
                     DLLDERFREHALRVIKGDVDAAVDMLGTTLDLPIYYTNGTHVTPAAPVPLTKRIEIEH
                     IFFPIVDGGNIFHVWLGEARPDPRGLMEMAMNLCRTTQIGYFAFTRDLTVSLKEYREL
                     KPAHPRPGRAAGFSPAADRADA"
     assembly_gap    4819..4918
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4919..>6164)
                     /gene="nrdD"
                     /locus_tag="PLV90_08050"
     CDS             complement(<4919..>6164)
                     /gene="nrdD"
                     /locus_tag="PLV90_08050"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOF97105.1"
                     /translation="KADKISKEQYLLQLPPDLADHHLRGDLHIHDLEYFGTRPFCIDG
                     STVVPLRMENRIRSLRLDELPIDADTWFPEDLAALTPKGWRRVAKVTRRKVDPGEMRR
                     IRTSGGRSLLVTGEHRIPIKAEDGMAIRRADEVRPGDALYRISGSDALRGETIESIDL
                     VRELAASVPANLLENVYVRGAEEIFSHVVQSGQAASYAGISRELGVEHQKQWYTRGIM
                     PVALFSAFSRRYGIEDYAGVTIGVAGSEHELPALLTLTPELVRLLGFFVSEGNYNAAP
                     AVGQYNLAITENRQAPAIQAAARAALNTYATIAGSTPDTTNIYGIEMEHNRGLQVSIG
                     GKAGYLLFRHVFGIPEGAAGKRLPWIVYHLDDVLLHEFLSALFTGDGSAYYRPEKSDC
                     IVNYTTASPALRQELALLLTALG"
BASE COUNT          971 a         1904 c         2087 g         1102 t
ORIGIN      
        1 cggacaaaag agccccggaa cctcctggat ggggaacggg ctgtctggga gttctcggtc
       61 accacccatg tcggccggct gatgctcgag aacctcacgc tccctgagga gcggctcatc
      121 accgacctga cgtcgtatcc cgatgccttc gcgtttcggg cctacgctct tgccggcacc
      181 caggagatcc cctcgatggg gctctccgat ccggtgattg ccatccggtc tgccggaagt
      241 gcggccgcgg agtcctctct tcctcctctc tacacgcttg tcggagttca ggactcactc
      301 cttgacccat cggagacgat ggtcgatgcg tggcggcggg gcatcgacta tgacgcgatc
      361 accgccgggg ccggagagca cctcggcact ctcgcgagcc tcgggaagga ggaggtggcg
      421 gccatctaca ccgcagaagg aagagccggg gcggaggcgg cggccggcac ctcgccgtcg
      481 ataatcgatc tgatcgtgga gttcttccgc agtatcttcg gagaggtttg agggtgaacc
      541 ccctcacccc tcccggaact gcttccagag gtgcatgagc ccgaagcatc ccgagagcat
      601 catgtccccg aagggtgctg cctggcaggc ctccgggagc agccggagcc ggtttgggcc
      661 cgtgacggcc agggcatcgg tgggaagggg ttttctgacc gcagccccgt ggccgccgtc
      721 atcaaagatc tcttctgacg tcagcgtgcc gtcggcgagc cgccggatgt agtgccggag
      781 tttggccggg gtgagggcgc cggtatggtg ctcgaagatc ccatagatct cccgcccttt
      841 cagggcaaag cagagggtat gcccgttccc ggcgttgacg aggatcactc ctttatctgc
      901 catacgccgg acttcggggt cgcagagcgc cccgatgagc gccaccggcc cggtatcggt
      961 gacgagcgcc ccgggcgcct ggcgccggac ggcctgcatc ctggtcatgg cggcgagcgg
     1021 ggggtcggag acgaacgaga ggagatccca gtccccggca tccagccgct cccgcaccag
     1081 ttcgaaccgg tggatgcggt tgctccggtg cggggagtag ccgtggtcct ggaccgcgac
     1141 cgcgacgttc tccgggtacg cgatgccaaa gagggagagt gccgcccgga gttctgactc
     1201 catgtaatcg gtggtatgga cgaccaccgc atcctccggg ggattgggct cgatagcgac
     1261 ccccatcgac cggaccctct ccggatcgtc gtggagggtt ccggcggcat cgggagtggc
     1321 gtagacgggg aggcctgccg ccaggtgctg ccgtaccgcg ccggtgttcg cgccgccgcc
     1381 catcaggaac ccctcgagga agatcgggcg gccggcccgg gtcgcccggc ggatccgatc
     1441 ggccgtcacc accgtcggag aggggaggac cagtttgatg ctgttctcga tagaacggcc
     1501 gggttcgtag accaggatat cctgggtgcc cctgccgacg tcgatcgcaa gaagcggtgt
     1561 cgtcagatcg atcatgagtg agcctcgcac gccgccgtcc tcacgacagg cggccttaac
     1621 aggatctctt ttaccgggac taaaaaggtg tgatgccggt caggcgtccg cccggtctgc
     1681 cgccggcgag aaaccggccg ccctccccgg gcgggggtgg gctggcttca gctcccggta
     1741 ttcttttaaa gagaccgtca ggtcgcgggt gaaggcgaag tagccgatct gggtcgtccg
     1801 gcagaggttc atcgccatct ccatcaaccc ccgggggtcg gggcgcgcct ccccgagcca
     1861 tacgtggaat atgttcccgc catccacgat cgggaagaag atgtgctcga tctcgatcct
     1921 cttcgtgagc gggaccgggg ccgccggggt gacgtgggtc ccgttcgtgt agtagatggg
     1981 aaggtcgagc gtcgtgccga gcatatcgac cgccgcatcg acgtcgccct tgatgacccg
     2041 gagtgcgtgc tcccggaacc gctcgtcgag gagatcggcg accgcgaacc gctggccggt
     2101 cgtctccgcc ggggtgcggg cgagggcgat cgtcatgttg tgcttctgcg agagttcccg
     2161 ggcgtacatc tcgagctccg tcatggcccg gacggcgagg cggaacgcct cctttgactc
     2221 gtggagctgg tgtccggtga agtgctgcac catctcgttg acgccgacca cgccgatggt
     2281 gtagacgagg ccttcgaggt ccacggcgac cgccccgcgc tcgccggtgt tcggatcttt
     2341 cgggcgctgc atcgcaaacg gcatccgccc gttcgcccgg atcagcgaca tccaccggcg
     2401 cttgatccgg tagaggccga cggcggtgtc catgagcgcc ttcagttcgg caaagagccg
     2461 ttcctggtta ccttccgcct tgtacgccgc ccgcgggcag ttgatggaca tcacctgcca
     2521 ggaacccatc gagaagtgtt ttccctctct gaagtggagt ttgtcctcga actcgtcgtc
     2581 ctcgtcggca agggaggaga actggtaggc gcagcagttc ccggtcacga tgccgagggc
     2641 gtttgcgaag gtgtgcgtgc cggcaacgtc gacaaggtcg tagacatggc catcgtagac
     2701 taccggctcg actgagcgga cccggagcgg gtggacgtca cccccggcga accggccctt
     2761 cccggctggt tgggtgatag gcgggaggaa gagcgagacc tggaccccct ccaccccgcc
     2821 ggcgctgtac ttgcttgcgc aggcgagggc cgtgtacccg cgagcacgtt cgccctccgg
     2881 gaggcgggtg aaggccccgc ccctctctct cccgaggggc atctctgcag ggaacccggt
     2941 tgccgcggag aaccgttcga tctggtcctg ggccgtgatg ctgcaggtgt aggtcgtctg
     3001 tggggcaccg ggccggtgaa tcccgtgttc ccgttcggcg taactcatct ggatgccaag
     3061 ctgcccggcg agccagacca ccttccgtgc ggcatcctcc gaggccgtct tcagcctgat
     3121 gctcctggcg cggcgcccga tggttctttt cccggtggcg aggaaggctg caccgaggta
     3181 gtcgccggcg aggatgcggt cgaggttgaa gagcaggtcg ggcaccgatc gtgtttcagc
     3241 cgaggaaccg cacccgagag cgtcgaccag gaggaagatg agtttactcc tgacgacaac
     3301 gtcgatccct gtcgtcgact tctggatctg tggttcgtac ccgagggcgg cgcggataca
     3361 ggcggcggca tccgctgcgc tatccgcatc atgccttcca aacgcgagtc ggaccgtgta
     3421 gtgccgtccg gcgtggtcgc tcgaccctgc cgcaagatag tagccgagga acaccgcgag
     3481 ttcggggctt accgggaggg tgcggggcag gccggggtgg cgggtgcccg ggatcgtcac
     3541 cgcatcttca gtgacgcgga tcgcaccggc gtggcctgcc ttcgtgagaa cctctgcgag
     3601 atcgatgtct gtaacgctgc cctcaccgag tccctctgcc gtgagcaccg gcaggtaatc
     3661 gccggcctcg aggtctcccg ccgtctctct ggcaaatccg cctccgttca ggacgtaaac
     3721 cgggtggtcg ggggtgacgg ttatctgccg gcccgactca agcgtgatcc ggaggaggtc
     3781 gccggtatac cgccggcgca tcacgccgcg gaacgggcga agcgatgcct ccagggtcgt
     3841 gaaatcgacg ctcggcgtct tcccgtcata gtaggcaagc tccgtgccga acgggtcgac
     3901 ccgacgcccg ttctttgccg cggcatcaaa gaggctccgg atcgatcgca cgacgaccct
     3961 gccggtgctg tcggccaccg ggaccagttc gtcgcccgcg aggcactggt agcacgatat
     4021 gccttctccg gccccccggt aggctggaat ctggttgtcg tagtaggggg tgccgaactt
     4081 cgacgcgagc tcaaacgtca tcaggtagag gtcgtggtag gtggggagat ccgggtgttc
     4141 gcgattatac gcctcgttct cgatgaggaa gtcgggctcg atgctgatct cgggtttcgg
     4201 gaaggagaag ggcttgcccc agtagtctcc ctcgagcatc acctccatca gcgccttgaa
     4261 gaggagcctg acctcccgct cgaactcgcc gtaggtccgg agcggcgcct ggttgccgtc
     4321 ccagatcttg ccgcggtaga cgcagggttt gtcccgccag agggtcggga cgcccgggga
     4381 gagctggacc gacgagaaga ccacctggcc gcctctcgcg accatcatct gggtcatctc
     4441 gtagacgaac atctgcatca attgcttgat ctccccgtac tccatcccct cgaagaaggg
     4501 tgccatgaat gtcaggaagt tgtagtagcc ctggccgccg gcgaagttcg tctgcgcgga
     4561 gccgagcgcc ttgacggcgt ggaggacggc cacctcggcc cgcttcgcgg ggccggcgac
     4621 cgacgccttc gtcccgttgc cgtccggcat caggccgtag tagaagaagt agcggaggtc
     4681 ccagtcctgg cagttgtgga tgaggaggcc gtcggaggcg tagaacgtgt ggctctcctc
     4741 ggtcccgtcg ccgtcgatga agaggtcgta gacgtactca ccggtgggct cagccggact
     4801 gacatcgacg acgatctcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc
     4921 gagcgcggtg agcaggagag cgagttcctg ccggagcgcc ggcgacgcgg tggtatagtt
     4981 gacgatgcag tccgatttct ccgggcggta gtatgcggag ccgtcgcccg tgaagagggc
     5041 ggagaggaac tcgtggagga ggacgtcgtc cagatggtag acgatccagg gaaggcgttt
     5101 tcctgccgct ccctccggga tgccgaagac atggcggaag aggaggtagc ctgccttccc
     5161 gccgatggag acctgcaggc cccggttgtg ttccatctcg atgccgtaga tgttcgtggt
     5221 atcgggagtg cttccggcga tcgttgcgta ggtgttcagg gcggcgcgtg ctgccgcctg
     5281 gatggcgggc gcctgacggt tctcggttat cgcgaggttg tactgaccga ccgccggggc
     5341 ggcgttgtag ttcccctccg agacgaagaa cccgaggagc cggacgagtt ccggcgtaag
     5401 ggtgaggagg gcggggagtt cgtgttcgct ccccgcgacc ccgatggtga ccccggcgta
     5461 gtcttcgatg ccgtagcggc gtgaaaaggc gctaaagaga gcgaccggca tgatcccgcg
     5521 cgtataccac tgcttctggt gctcgacgcc gagttcccgc gagatcccgg cgtacgaagc
     5581 ggcctgcccg ctctgcacca catggctaaa gatctcctct gcaccgcgga cgtagacgtt
     5641 ctcgaggagg ttggccggca ccgacgccgc gagttcgcgg acgagatcga tcgattcgat
     5701 cgtctccccg cggagggcgt cgctcccgct gatccggtag agggcgtccc cgggccggac
     5761 ctcgtcggcg cggcggatgg ccatgccgtc ctctgccttg atcgggatcc ggtgctcgcc
     5821 ggtgaccagg agcgaccggc cgcccgatgt ccgaatccgg cgcatctcgc cggggtccac
     5881 cttccggcgg gtcacctttg caacccggcg ccagcccttc ggcgtgagcg cggcgagatc
     5941 ctccgggaac cacgtatcgg cgtcgatcgg gagttcgtca aggcgaagac tccggatccg
     6001 gttctccatc cgcaggggca cgaccgtgct cccgtcgatg cagaacgggc gcgtcccgaa
     6061 gtattccagg tcgtggatgt ggaggtcgcc gcggaggtgg tggtccgcaa gatcgggagg
     6121 gagctgcagg aggtactgct ccttgctgat cttgtccgcc ttct
//
LOCUS       DAOSII010000106         6038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_11835_length_6038_cov_44.052983, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000106 DAOSII010000000
VERSION     DAOSII010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6038
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11835_length_6038_cov_44.052983"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PLV90_08055"
     CDS             complement(<1..310)
                     /locus_tag="PLV90_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97106.1"
                     /translation="MAASDSTELSKTQADYVCDGVDDHAEIQAALAALPEGGKVVLSN
                     GTFNCAGVIAPAGGTTLSGQGPDATNLVFTRDGRITIDREHVTLDGFGVKGSGYSSGV
                     K"
     gene            928..2103
                     /locus_tag="PLV90_08060"
     CDS             928..2103
                     /locus_tag="PLV90_08060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOF97107.1"
                     /translation="MRVACLTFWFYDYTIQMANELARHTEVLLLLPDYRTDEYLESID
                     PEVKIRIFRYSRYAGRLGPSCYPVIRDIADAIHDFRPDIVHFQVNNPMLCPLIPMLRR
                     YPLVATFHDIEPHPGEDRLLDIGSLLYRLTLFMSRALTDRMFVHGRALKDILVANYRV
                     PEERVHVIPIGEHEVAPFVKFEEEGREPEGLRVLFFGRIHRYKGLDCLIQAEPLITQK
                     IPGARIVIAGTGEDFGRYEEMMADRDVFEVYNYRIPYEEGARLFQQASVVALPYIEAS
                     QSGVIPTAYGFKRPVVVTDTGSLPEIVDDGETGYVVPPRDPEALAGAIVRLLEDPAAC
                     RRMGERGYMKLKTDMAWPTIVRSILPVYRDLAPAPGREETPADRTFTGRAPAAGKER"
     gene            2320..4527
                     /locus_tag="PLV90_08065"
     CDS             2320..4527
                     /locus_tag="PLV90_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2206 domain-containing protein"
                     /protein_id="HOF97108.1"
                     /translation="MRLPDPFQMNDWENRRFFWAFQAIQLAFIGIVCLDLIGYHIPVA
                     REALAFVYLTFLPGILVLKALRLHDLGTIETVLYSVGLSLVVLMLTGLFANTVYPLLG
                     SARPLSLEVLFPTIVGVVQILLYLALARDREHAGSSHAGLEISIPPPAVPFLALLPFL
                     AIAGTYIRNEYHMVTLLFLLLVLIAVIGLVVGFDRFIPASCYPFAVYSLALALLYHTS
                     LISGYIWGYDIHHELHLASNVLISGIWDMTIPYNTNGMLSVVMLAPIYSLICDLDLVW
                     VFKILYPLFFALVPLALYRVIEKQTNPKIGFFSVFFFMSFFTFYTEMISLARQQVAEL
                     FLVLTVLAMVDKSMDRSRRAFLMITFGFAMIVSHYGLSYIYLFSLVPAWLLLILPDYL
                     PSGILEKLRGMAGTLAHDPLASHEPRPRLQTLVLPYIFILAILTYLWYSTVAEGTAFA
                     TITGIGDKIVNTLFAEPFNPATIQGMYILASQSVTPLHSLAKVVHIATQGLIAVGLLA
                     TLARRGRWRIEPEYLAISIVFFLVNIAGIAVPFFASSLNTSRLYHITLILLAPFAIIG
                     GIALYEAVAGRVRAITALPLMRTAFQALSVFFVVFLLFNTGLIYQVTGDSPTSMALET
                     SGDKPVFNNKEVQGARWLISEGSGRPIYVDGMRWWLLLGFNPDNQRYLPANASYIEPG
                     SYLYLGTYNLVRESVRIEVRERAVTAADYTDAGRFTENQNRIYDNAGSSVYYR"
     gene            complement(4617..5858)
                     /locus_tag="PLV90_08070"
     CDS             complement(4617..5858)
                     /locus_tag="PLV90_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOF97109.1"
                     /translation="MAVTLPKNFSEVKRHLQEPLIRNSFFIMASSLAAAGFGFFFWMI
                     AARLYSQAEVGVATALMSSMGLLILISRLGLDQSVIRFFPSRDKNGVLGTAIIVPTAV
                     ALGAGLIFIAAVEVISPELAVVRTVAPLYLAFLGAYSVTWVLEGAFNALRKSEHYFAL
                     NLLYGSRILFLIPLVFLGAMGIFSAFGLSFVLGLALALVLLARCSIRPTPGIDRVFLR
                     EAWQFSAGAYIAGMLISAPNMIIPIMVLHVLGAESTASYYITYAIVSILFMIPYAFTT
                     SLFVEGSHGGEMKRSVLRTLASMFALLIPAIIGLSLFGEQILNLIGKDYVEGVALLQV
                     LALSALFVSVCQTFISIAKVRSDIKSLILLSGFVSVALLGLGYTLMQRFGLIGMGYAW
                     LGTYAAGTLLVGLILKRKGWM"
BASE COUNT         1199 a         1928 c         1761 g         1150 t
ORIGIN      
        1 acttgacacc gctgctgtag ccgctgccct tcacgccgaa cccgtccagg gtcacgtgct
       61 cacggtcgat ggtgatgcgg ccgtcgcggg tgaagacgag gttcgtcgca tcgggcccct
      121 gcccggagag cgtcgtcccc cccgccgggg cgatgacgcc cgcacagttg aacgtgccgt
      181 ttgagaggac gaccttgccg ccttccggca gtgcggcgag cgctgcctgg atctccgcat
      241 ggtcgtcgac tccatcacag acatagtctg cctgggtctt tgacagttcg gtgctatcgc
      301 ttgcagccac ggtgagggtg gccggagttg cagacccggc cgcgcttacc ggagagaatg
      361 ccggtataag gcagcacacc aggagcagtg caacgagtcg tggtacatat cgcctgtcta
      421 tcatgccatt cagcctctct gtgaggtaga actaccgtct tttgataacg ctataaatat
      481 ttttttgaaa gaataaaact cgtgaaaggg gatatttagc catataatcc ctccggggtt
      541 cgctcatcgg gtgaaagtca ttcgagaggg aatataggat aatgcaaaag ggtattatat
      601 cattaccaaa attaaaatca aaacgtatta atactcgcgc tttcgccatc gcctgcagga
      661 gatcggccat cccctgcacg catttgcgta aggctgatgg agattttgcc tcgtgcgccg
      721 gcacgacccg cgacctccgg ccgctaccgc ccgccgacgg cccggcacac ccgggtcggg
      781 catgcgggcc agccttcagg agcgtgcagg agagcacccg tggatgaggg gagcccccca
      841 ttcaccgctg ccggtcgcac gccgtcagga ccaacggccc cgggagacat aacatttata
      901 taagagatct ccgcagagac tgcccctatg cgagtggcat gcctgacctt ctggttctac
      961 gactacacca tccagatggc aaacgagctc gcccggcaca ccgaggtgct gctgctcctc
     1021 cccgattaca ggacggacga gtacctggag agcatcgacc cggaggtaaa gatccgcatc
     1081 ttcaggtact cgcgatacgc gggaaggtta ggcccgtcct gctacccggt gatccgggac
     1141 atcgccgatg caatccatga cttcaggccc gatatcgttc actttcaggt gaacaacccc
     1201 atgctctgcc cgctcatacc gatgctcagg aggtatcccc tggtggcgac gttccacgac
     1261 atcgaaccgc accccggcga agaccgcctc ctcgatatcg gctccctgct ctaccggctc
     1321 accctgttca tgtcgcgggc cctgaccgac cggatgtttg tccacgggag agcgctcaag
     1381 gatatcctgg tggcaaacta ccgtgttccc gaagagagag tgcacgtcat cccgataggc
     1441 gagcacgagg tggcgccgtt tgtgaagttc gaggaagagg ggcgggagcc cgaaggcctc
     1501 agagtcctct ttttcggccg catccaccgt tacaaggggc tcgattgcct gatccaggca
     1561 gaacccctca tcacccagaa gatccccggc gcccggatcg tcatagccgg cacaggcgaa
     1621 gatttcggga ggtacgagga gatgatggcg gaccgggatg tattcgaggt ctacaactac
     1681 aggataccct atgaagaggg cgcccggctc ttccagcagg caagcgtcgt cgcgctcccg
     1741 tatatcgagg catcccagag cggcgtcata ccgaccgcat acgggttcaa gaggcctgtg
     1801 gtggtcaccg atacgggaag cctgccggag atcgtggacg acggagagac cgggtacgtc
     1861 gtgcctccac gcgacccgga ggccctcgcc ggtgcgatcg tgagactgct tgaggacccg
     1921 gctgcgtgcc ggcgcatggg agagcggggc tacatgaagc tgaagacgga tatggcatgg
     1981 cccacgatcg tacggtccat ccttccggtc taccgcgacc ttgcgcccgc tccggggaga
     2041 gaggagaccc ctgcagacag gaccttcacc gggcgagccc cggccgccgg gaaagagcga
     2101 tgaatgcagg cggcagccct gctcgaccgg tggtgcgttg cagagcgccg gcagcccctg
     2161 ccgcaggcgg agatatcccc ggggagcggt ccgccggcac aaaggtttaa taggcgggcc
     2221 cgatagtagt gcacgataca gagacctgat ggagacctga atgagaagat cccgcccata
     2281 tccgcaaaat cggtcgccga ctgatccccc ggaggtacaa tgcgcctgcc cgacccgttt
     2341 cagatgaacg actgggagaa cagaagattc ttctgggcat tccaggcgat ccagctcgct
     2401 ttcatcggta tcgtctgcct cgacctcatc ggctatcata taccggtcgc acgggaggcc
     2461 ctggccttcg tctacctcac cttcctgccg ggcatcctcg tgctgaaggc gctcaggctc
     2521 cacgatctcg gcacgatcga gacggtgctc tactcggtcg gactgagcct cgtggttctc
     2581 atgctcaccg gcctcttcgc caacaccgtc tacccgctgc tcggttctgc acgaccgtta
     2641 tccctcgagg tcctctttcc gacgatcgtc ggcgtcgtgc agatcctgct ctacctcgcg
     2701 ctcgcccgtg accgggaaca tgccggctcg agccacgcag ggctggagat ctccatcccc
     2761 ccgcccgccg tgccgtttct cgcgctgctc ccgttccttg cgatcgccgg gacgtacata
     2821 cgcaacgagt accacatggt cacactcctc ttcctgctcc tcgtcctgat cgctgtcatc
     2881 ggcctcgttg tcgggttcga ccggttcatc ccggcatcct gctacccatt tgccgtctat
     2941 agcctcgcgc tcgcgctcct ctaccacaca tcgctcatat caggatacat ctgggggtac
     3001 gacatccacc acgaacttca cctcgcaagc aacgtgctga tatcagggat ctgggatatg
     3061 accatcccct acaacacaaa cgggatgctc tcggtcgtta tgctcgcccc catctattcg
     3121 ctcatatgtg acctggatct cgtctgggtc ttcaagatcc tctaccctct cttcttcgcg
     3181 ctcgtgccgc tcgcgctcta ccgggtgatc gagaagcaga cgaacccgaa gattggattc
     3241 ttctcggtct tcttcttcat gtcgttcttc acgttctata ccgagatgat ctcccttgcc
     3301 cgccagcagg tcgcggaact cttcctcgtg ctgaccgtgc ttgcgatggt cgacaaatcc
     3361 atggaccgga gcaggcgggc gttcctcatg atcaccttcg gttttgccat gatcgtctcg
     3421 cattacgggc tctcgtacat ctatctcttc tcgctcgtac cggcatggct gctgctgatc
     3481 cttcccgact acctgccgtc cggcatcctg gagaagctcc gcgggatggc gggcaccctg
     3541 gcacacgacc cgctcgcctc gcacgaaccc cggccacgcc tccagacgct cgtcctcccc
     3601 tacatcttca tccttgctat cctcacctac ctctggtact cgacggtagc cgaaggcacg
     3661 gcatttgcca ccatcaccgg catcggggat aagatagtaa acaccctttt tgcggagcct
     3721 ttcaaccccg cgaccatcca gggcatgtac atcctcgcca gccagtccgt cacacccctg
     3781 cacagcctgg caaaggtcgt ccatatcgca acccaggggc tcatcgccgt ggggctgctg
     3841 gcaacccttg cccggcgcgg caggtggcgg atcgagcccg agtatctcgc catatcgatc
     3901 gtcttcttcc tcgtcaacat agccggcatc gccgttccct tcttcgcaag ctcgctcaac
     3961 accagccggc tctaccacat caccctgatc ctccttgccc cgttcgcgat catcggcggc
     4021 atagcgctct atgaggcggt cgccgggagg gtccgtgcga tcacggccct gccgctcatg
     4081 aggacggcat ttcaagcgct ctcggtcttc ttcgtcgtct tcctcctctt caacaccggc
     4141 ctcatctacc aggtcacggg cgacagcccc acgtcgatgg cgcttgagac ttccggggac
     4201 aaaccggtct tcaacaataa agaggtgcag ggggcgcggt ggctcatatc ggaagggagc
     4261 ggacggccca tctacgtcga cggcatgcgc tggtggctcc ttctcgggtt caacccggat
     4321 aaccagcgat atctcccggc aaatgcgtca tatatagaac cgggctccta cctctacctc
     4381 ggtacgtata acctcgtgcg ggagagcgtc cggatcgagg tgcgggagcg ggcggtgact
     4441 gcggcggact acaccgatgc cgggaggttc accgagaacc agaaccggat atatgacaac
     4501 gccggttcct ccgtctacta ccggtgaggg ggcggccacc gtgtggccaa agccgggttt
     4561 cacggatttc cgggggtgcc ggagggggaa gagacctccc ggcctgcacc ccgaagttac
     4621 atccaccctt ttctcttcag gataaggccg acgaggagcg tacctgccgc atacgtgccg
     4681 agccacgcgt agcccatgcc gatgaggccg aaccgctgca tgagggtgta accgagcccg
     4741 agcagggcga cgctgacgaa tccgctgagg aggatgaggc ttttgatatc gctccggacc
     4801 ttggcgatgg atatgaaagt ctggcagacg gagacgaaca gggcggatag cgcgagcacc
     4861 tggagcaagg ccaccccttc gacgtaatct ttgccgatca ggttgaggat ctgttccccg
     4921 aagagggaaa gcccgatgat cgcaggtatg agcagggcga acatgctcgc gagcgtccgg
     4981 agcacgctcc tcttcatctc ccccccgtgg ctcccctcaa cgaagagcga tgtcgtgaac
     5041 gcgtacggga tcatgaagag gatcgagacg atggcgtagg tgatgtagta actcgcggtg
     5101 ctctcggccc cgagcacgtg gagcaccatg atcgggatga tcatgttcgg ggcggagatc
     5161 agcatcccgg cgatgtatgc gccggcggaa aactgccacg cctcccgcag gaagacccgg
     5221 tcgatacccg gtgtcggccg tatggagcac cgggcaagga ggacgagggc aagggcgagg
     5281 ccgaggacga acgagaggcc aaaggcgctg aagatcccca tcgccccgag gaagaccagg
     5341 gggatgagaa agaggatccg cgacccatag agcaggttca aggcgaagta gtgttccgat
     5401 ttccggagag cgttgaacgc accctcaaga acccaggtga cggagtatgc cccgaggaag
     5461 gcaagataga gtggcgcgac cgtcctgacg accgcaagct cgggcgatat gacctcgacc
     5521 gcggcgatga agatcagccc tgcaccgagc gccaccgctg tcggcacgat gatggcggtc
     5581 ccgagcaccc cgttcttgtc gcggctggga aagaaccgga tcaccgactg gtccaggccg
     5641 agacgggata tgagtatgag gagccccatg gacgacatca aggcggttgc aacgcccacc
     5701 tccgcctgcg agtagagtcg tgccgcgatc atccagaaga aaaatccgaa tcccgccgcg
     5761 gcaagcgacg acgccatgat gaagaacgaa ttccggatga gcggttcctg caggtgccgt
     5821 ttgacttcac tgaagttttt ggggagcgtt actgccatcg gccgccatta tcgggcggta
     5881 cctatataga tgtatccacc gtcccggccg cctgcaggtt gcagggcggg cgaggggggt
     5941 tctgcgaggc tctgccgccc aaaacaagcc cgggagacct ccgcacgatc gatactccac
     6001 caaaccgccc cgtgaggtct caccgggcgc atgcccga
//
LOCUS       DAOSII010000107         5996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_11938_length_5996_cov_42.070527, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000107 DAOSII010000000
VERSION     DAOSII010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5996
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11938_length_5996_cov_42.070527"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="PLV90_08075"
     CDS             complement(<1..754)
                     /locus_tag="PLV90_08075"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HOF97110.1"
                     /translation="MIKVAINGYGTIGKRVADAVAKQPDMEVVGVSKTSVSAEAFVAK
                     KRGYPLYIADIGKKAAFEKAGLDVAGDVEAMLKAADIVVDATPGGVGEKNRPVYERLG
                     KKAIYQGGEEHEVAGFSFNAHVNYKEAEGHQFARIVSCNSTGLVRIISAMDQAFGVDR
                     VRAVMVRRASDPGDVKRGPVDATVLNPATIPSHHGPDVNTVLPDVNIVTLAMIVPTTF
                     MHMHAIQMDLKKETTREEVLKVFENHSRIGLVR"
     gene            1036..1581
                     /locus_tag="PLV90_08080"
     CDS             1036..1581
                     /locus_tag="PLV90_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF97111.1"
                     /translation="MENQPILATDRLLLRSFALADAFTVQQLAGDYAVAATTLLPHPY
                     PDGLAELWIASLREGIERGDTAAFAITLAGEGRLIGGVRLRIDCGHRRAELGFWVARS
                     CWGRGYATEAVRAMIEYGFSALGLHRVYAMHFSRNPASGRVMEKCGMVHEGRFREHIR
                     KWGVFEDVDIWGILREGGAFP"
     gene            1578..2159
                     /locus_tag="PLV90_08085"
     CDS             1578..2159
                     /locus_tag="PLV90_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF97112.1"
                     /translation="MTGQPILVTDRLLLRPFTLADASTVQRLAGEYAVASSALEIPYP
                     YPDGVAEAWIATHRPGFERGVHAIYAVTCDGDLVGAAGLVEIDRDHRRAELGYWMGRP
                     YWGRGYATEATRAVIEYGFSVLGLHRVYAMHFSRNRASGRVMEKCGMVHEAHLRGHVR
                     KWGVVEDIDVCGVLHDEWRERCTGPAIRECRAG"
     gene            complement(2170..2379)
                     /locus_tag="PLV90_08090"
     CDS             complement(2170..2379)
                     /locus_tag="PLV90_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97113.1"
                     /translation="MERRGRAEWIKIRQRRVIDEKEYYADRLYETRRDATSYGRMLQN
                     QDKDVLILGTIHEGGHRGYAVFVRR"
     gene            complement(2462..2728)
                     /locus_tag="PLV90_08095"
     CDS             complement(2462..2728)
                     /locus_tag="PLV90_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97114.1"
                     /translation="MHDHYSTRGRKAMGGRLGITWKEFDLEQFRRGMVVELEHGLHDP
                     VTNVTDDDLFLTAKTALAHLNEFPDNYDRLEKQAEAYRAERRAA"
     gene            2792..3112
                     /locus_tag="PLV90_08100"
     CDS             2792..3112
                     /locus_tag="PLV90_08100"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOF97115.1"
                     /translation="MHPSFRTALDAFLAKAIYRWGDRIEEVILFGSTARGEATPSSDL
                     DLLVITNEEDFRLRRSLIGLAYDIMLETGVNLSVKVLSRGDLERRRNSPFLKAVPAEG
                     ARVG"
     assembly_gap    3816..3915
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3960..4172
                     /locus_tag="PLV90_08105"
     CDS             3960..4172
                     /locus_tag="PLV90_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HOF97116.1"
                     /translation="MQRQAVESTNIRSVGYNEEDEILEVEFHSGGVYNYVGVPPEVYE
                     GLLAARSKGRYFGDFIRLRYPYEKVR"
     gene            4228..4470
                     /locus_tag="PLV90_08110"
     CDS             4228..4470
                     /locus_tag="PLV90_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HOF97117.1"
                     /translation="MSQPSAGSSAGGIRSIGYDPTTKALLVICESGDAYRYAGVRKEV
                     YESLLAAPAREQFVRESLEGRYPREKYPCMAVGEGI"
     gene            4547..5479
                     /locus_tag="PLV90_08115"
     CDS             4547..5479
                     /locus_tag="PLV90_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOF97118.1"
                     /translation="MTTVIAVDLGGTNLRAALIDSADATILAHTAAPTPASGPSGDAI
                     TAAIIDRIETLLASAPGENATAIGVASAGPLDLDRGWVVNSPNIAFPVVEIVEPLSER
                     FDLPVALINDARAGVLGEWWAGAARGSDNVVYVTLSTGIGGGAVVNGRLLLGATGNAG
                     EIGHIPVDTRYNLTCGCGYKGHWEAYASAKNLPGFFAAWRKEADVRDVAFDAGSTRAI
                     LEAVRKEDPVALAFMEALGQVNARGVSAVIVAYNPDIIVFDGPLARYHGDIVIRHMEP
                     YIDRYLALPRLAVSELGGRSPLLGAAAYALEVRR"
     gene            5476..5985
                     /locus_tag="PLV90_08120"
     CDS             5476..5985
                     /locus_tag="PLV90_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HOF97119.1"
                     /translation="MIEIYQNLYVGTQEDYEVTVEAHETWCVVHACRSPYHCLAVTCS
                     PVGTVPEDHPEHLVARRGNRLMLNLVDTHNPDDVPKEAIDAALLFIDRCLAKGRPVLV
                     HCGFGISRSAAIGLLYLAAHTDVLPVESLAAAEAAYRRIYPAYRPGRGIRGFLAAHWD
                     EYAKRRVTA"
BASE COUNT          999 a         1853 c         1895 g         1149 t
ORIGIN      
        1 tgcggacgag cccgatgcgg ctgtggttct caaagacctt cagcacctct tcgcgagtgg
       61 tctccttctt caagtccatc tggatagcat gcatgtgcat gaaggtcgtc gggacgatca
      121 tcgcaagagt gacgatgttg acgtcgggga ggacggtgtt gacgtcgggg ccgtggtggc
      181 tcggtatggt ggccgggtta aggacggtgg catccacagg cccccgcttg acgtcgccgg
      241 ggtctgatgc ccggcggacc atcaccgctc tgacccggtc gacgccgaag gcctggtcca
      301 tggcagagat gatccggacg agcccggtgc tgttgcacga gacgatccgg gcaaactggt
      361 gcccttcggc ctccttgtag ttcacgtgag cgttgaagga gaacccggcg acctcgtgct
      421 cctcgccgcc ctgatagatt gccttcttcc cgaggcgttc gtagaccggg cggttcttct
      481 ctccgacacc gccgggggtg gcatccacga cgatatcggc ggctttcagc atcgcctcga
      541 cgtcgccggc gacatcaagg ccggccttct cgaacgctgc tttctttccg atatcggcga
      601 tgtagagggg atatccacgc ttttttgcga cgaacgcctc tgccgagacg cttgtctttg
      661 agacccctac cacttccatg tcgggctgtt tggcgaccgc atcggcaacc cgcttaccga
      721 tggtgccgta accgttgatt gcgactttga tcatgtatga tggaatacag aacaaaaatt
      781 aataaaggtt gctgatttgc agatagggcg gttatcatac aacttccggc caaacggaag
      841 ggcagggtgg ctcccaatcg ctaatgggcc acataatccc ggaagatggg gcaccagggt
      901 ataaccgggt gtggttatcg gggggttata cggccgggtg ggtggcggtt gccgtggtct
      961 ctggatcacc ctctccttgc ggtccgggtg gcggcgcggc atggcttata tccggccggt
     1021 gcaatgagca tgcatatgga gaaccagccc atcctcgcga ccgatcgcct gctcttgcgg
     1081 tcgttcgccc ttgcggacgc tttcacggtg cagcagctcg ccggcgacta cgccgttgcc
     1141 gcgacaaccc tcctccctca cccctatcct gatggccttg ccgagctctg gatcgcctcc
     1201 ctccgcgaag gtatcgagcg cggtgatacc gcagccttcg ccatcacgct tgccggggaa
     1261 ggaaggctga tcggcggcgt ccgcctgcgg atcgactgcg gccaccgccg ggcagagctc
     1321 gggttctggg tcgcacggtc ctgctggggc agagggtacg ccaccgaggc ggtccgggcg
     1381 atgatcgagt atgggttctc ggcgctcggg ctgcaccggg tctacgccat gcacttctcc
     1441 cggaaccccg cctcgggccg ggtgatggag aagtgcggaa tggtgcatga aggccgcttc
     1501 cgcgagcata tcaggaagtg gggagtcttt gaggatgtcg atatctgggg gattctccgg
     1561 gagggaggcg ccttcccgtg accggacagc ccatcctcgt aaccgaccgg ctcctcctgc
     1621 ggccgttcac ccttgcggat gcttccacgg tgcagcgcct cgccggcgag tatgcggtcg
     1681 cgtcctcagc cctcgagatc ccctacccct accccgacgg ggtggcggag gcctggattg
     1741 cgacgcaccg cccagggttt gagcgggggg tgcatgccat ctacgccgtc acctgcgatg
     1801 gagatctcgt gggcgcggcc gggctcgtgg agatcgaccg cgatcaccgc cgggcggagc
     1861 tcggttactg gatggggagg ccgtactggg ggcgggggta tgccacggaa gccacccggg
     1921 cggtgatcga gtacgggttc tcggtcctcg ggctgcaccg ggtctatgcc atgcacttct
     1981 cccggaaccg cgcctcgggc cgggtgatgg agaagtgcgg tatggtgcac gaagcgcacc
     2041 tccgggggca cgtcaggaag tggggggtcg ttgaggatat cgacgtctgc ggcgttcttc
     2101 acgacgaatg gcgggaacga tgcacggggc cggcgatccg ggagtgccgg gctggttgag
     2161 ccggctgcct tacctcctga cgaagacggc gtagccccgg tgcccgcctt cgtggatggt
     2221 cccgaggatg aggacgtctt tatcctggtt ctgcagcatg cggccgtagg aggttgcgtc
     2281 acgccgggtc tcgtagagcc ggtcggcgta gtactctttc tcgtcgatga cccgccgctg
     2341 ccgtatcttg atccattctg ctctcccacg ccgttccata tgccatgatt ggcgcggagg
     2401 ggcgatagtt gttgcgcatc gaccgcccgg cactacaggg gcgacagcga acgcccaatc
     2461 gctatgccgc tctccgttcc gcccggtagg cctccgcctg cttctcgagc cggtcgtagt
     2521 tgtcggggaa ttcgttgagg tgcgcaagcg ctgtcttcgc cgtcaggaag aggtcgtcgt
     2581 cggtgacgtt tgtcaccggg tcgtggaggc cgtgctcgag ttcaacgacc atgccgcgcc
     2641 ggaactgttc gagatcgaac tctttccagg tgatgccgag gcgcccgccc atcgccttcc
     2701 ttccccgggt tgagtagtga tcatgcacgg gtcaccggcc cttccacgcg aagatatatc
     2761 acccggaaga tgcgatacag gattgatacc catgcatccc tcgttccgca ccgcacttga
     2821 tgcattcctt gcgaaggcca tctaccggtg gggtgaccgc atcgaggagg tgatcctctt
     2881 cggctcaacg gcacgcggtg aggcaacgcc ttcatcggat ctcgacctgc tggtgattac
     2941 gaacgaggaa gacttccgcc tcaggcggag cctgatcggg cttgcgtatg acatcatgct
     3001 cgagacaggc gtaaacctct cggtcaaggt gctctctcgc ggcgatctgg agaggaggcg
     3061 aaattcgccg tttttgaagg cggtaccggc cgagggtgcc cgggttggat aagggcgcga
     3121 ctgttcacga attactccgg agagcagagc agcggcttgc tgccgcccga tatcttctga
     3181 acgagggatt ttgtgaagac cccgtgaacc gcgcgtatca ttccatgttt tttgcagcaa
     3241 cagcgctgct gctcacccgc ggaattacag tccggacaca caggggcctg atcgctacat
     3301 tcggttcgga gtttatccgg accggggtga tcgatccgga gttcggcagg gcatttcgca
     3361 tcgcggaaga acttcgggag gaggttgatt attcgatctc acggattctc tcagcagaag
     3421 aggttctggt cgttatcgac gatgcggaac aatttttaga ggtgatcaaa gagagatacg
     3481 gtgatcgctc cctccccctg tcagggaagt cccgggtttt ggcccggcga atgcagccct
     3541 caccccggta tgccagaccc ggattgcctc gataagagag attgctctat agaatccaca
     3601 tagagcatcc tccagcagct ttcctgcccc ggaaagccta tccggaagaa ccgccggata
     3661 acctgcatct ggcatcttta tccccctccg gcacctgcac acccgggacc gatgatgccg
     3721 gcagaccggg ggtgggggtg cgtgctcttt gtgctgccgg cgctcctgct ggtcgctatt
     3781 gcctctgcgg ccggcgtggc cgacccggcg ggggannnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnctccc cacaaccctt ataccccatc accgcctcca cccccgtcca
     3961 tgcaacgcca ggccgtcgag tccaccaaca tacgatcggt cggttataac gaagaagacg
     4021 agatcctgga ggtcgagttt cacagcggcg gggtctacaa ctacgtcggg gtgccgcccg
     4081 aggtctacga gggattgctt gcagcccggt cgaaaggccg gtacttcggg gatttcatcc
     4141 ggctgcgtta cccctacgag aaggtccgct gaccggcacg atcaccggcc gcaaccgcta
     4201 tcatcgctgc cgaccacacc ggctcgcatg tctcaaccat cagcggggtc ttccgccggc
     4261 ggcatacgat ccatcggtta cgaccccacg acgaaggcgc tcctcgtcat atgcgagagc
     4321 ggtgacgcct accgctacgc cggcgtcagg aaagaggtct acgagagcct tcttgcggct
     4381 ccggcccggg agcagttcgt ccgcgagtcc ctggagggca ggtatccccg ggagaagtac
     4441 ccctgcatgg cggtggggga gggtatctga tccgggcgcg ggggcaggtt tatgcaacgg
     4501 gctcgcgtaa cctcgtgaag agaagcatct gctctccgga gaacccatga cgaccgtgat
     4561 tgccgttgac ctcggcggga ccaacctccg cgccgccctg atcgactccg ccgacgcgac
     4621 catactggcc cacaccgccg caccgacgcc ggcctcgggg ccttccggag atgcgatcac
     4681 cgccgcgatc atcgaccgga tcgagaccct cctcgcgtcg gcgccgggag agaacgccac
     4741 cgcgatcggc gtcgcgtcgg ccggtcccct ggacctcgac cgcgggtggg tcgtaaactc
     4801 accaaacatc gccttcccgg tcgtggagat cgtcgaaccg ctcagcgaac ggttcgatct
     4861 accggtcgcc ctgatcaacg atgcccgggc gggcgtcctc ggcgaatggt gggcaggcgc
     4921 tgcccgcggc tccgataacg ttgtctacgt caccctttcc accgggatag gcggcggcgc
     4981 ggtagtgaac ggccgtctcc tcctcggggc gacggggaac gccggagaga tcgggcacat
     5041 acccgtcgat acccgttaca acctcacctg cgggtgcggc tataagggcc actgggaggc
     5101 ctacgcctcc gcaaagaacc tccccgggtt ctttgcagcg tggcgaaagg aggccgatgt
     5161 ccgggacgtc gcctttgatg ccggttcgac ccgggcgatc ctcgaggcgg tccgaaaaga
     5221 ggatccggtc gccctcgcct tcatggaggc gctcgggcag gtgaacgccc ggggcgtctc
     5281 ggccgtgatc gttgcctaca accccgatat catcgtcttc gacggaccgc tcgcccgcta
     5341 ccacggggat atcgttatcc gtcatatgga gccttatatc gaccgctacc tcgccctccc
     5401 gcgtcttgcc gtctccgaac ttggggggcg ctcgccgctc ctcggggccg cggcgtatgc
     5461 ccttgaggta cgccgatgat cgagatctat caaaacctgt acgtcgggac ccaggaggac
     5521 tacgaggtca cggtggaggc ccacgagacc tggtgcgtgg tgcatgcctg ccggagccct
     5581 taccactgcc tggccgtcac ctgctcgccc gtcgggacgg tgccggagga tcaccccgaa
     5641 cacctggtcg cccggcgagg caaccggctg atgctcaacc tggtcgatac gcacaacccc
     5701 gacgacgtcc cgaaagaggc gatcgacgcc gccctgctct tcatcgaccg gtgtctcgcg
     5761 aagggccggc cggttctggt ccattgcggt ttcggtatct cccggtcggc ggcgatcggg
     5821 ctcctctacc ttgcggccca caccgacgtc ctgccggtgg agagccttgc agcggccgag
     5881 gcggcttatc gccggatata cccggcttat cggcccggtc ggggaatacg gggctttctt
     5941 gcggcgcact gggacgagta cgcaaagagg cgggtgacgg catgatcgtg gctacc
//
LOCUS       DAOSII010000108         5958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_12019_length_5958_cov_30.293749, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000108 DAOSII010000000
VERSION     DAOSII010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12019_length_5958_cov_30.293749"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="PLV90_08125"
     CDS             <1..731
                     /locus_tag="PLV90_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97120.1"
                     /translation="FSTTFNSSFVKRNRNIIVADEIDGAPLPKDGKTWPLKLVGSALT
                     SNKQRVGSIDEIVLTGVPVPVVPPEGDATITLSPGWNFVSTPKRLANGVNTLAIFDVV
                     DTAAHSIQFYDGLEQKWRRVAPTDPFQPLDAVWVYANTTCTVPLTFAPSVPDLPPAKE
                     LGRGWNAIGFTDTVPESAMGTLLSLGDRWSTLIGFDADAQKYDDSIIRTVNSGHHSVM
                     RSMQPTSGYWIYMTEEDTLAGISA"
     gene            745..>1149
                     /locus_tag="PLV90_08130"
     CDS             745..>1149
                     /locus_tag="PLV90_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97121.1"
                     /translation="MKYGIALILLLGFVTGVAGAGGQIPVLPHEFYGSVTIAGSPAPA
                     GTAITATVGGTECGSIQVVDAGRYGYLDWRLGGRLDVMATAGQAGETIAFSINGVAAQ
                     ETATFMPGKVTALDLSVPGTGGSDDNSSGDDNS"
     assembly_gap    1150..1249
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1250..1924
                     /locus_tag="PLV90_08135"
     CDS             <1250..1924
                     /locus_tag="PLV90_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97122.1"
                     /translation="SDSGAGSESGGGRSPGDAGSQFAHAPAAPEAQTGRASLATSPAG
                     VVLESVTVRTVDENCAVAIREKTTARDAAGNPLNEVTVARTADVPAAPPGTTVAIALD
                     CGPAGATFDPPALVTYTLSAEEWAKIDRGATPKVMWYNPGSGAWQDIPATVNLATRTV
                     TVEVSHFSIYALVWTVPEKGFAGAEGTPAGSPVGETGPAFPVPALVLVVVLIAALAAF
                     LVMRKK"
     gene            1947..4838
                     /locus_tag="PLV90_08140"
     CDS             1947..4838
                     /locus_tag="PLV90_08140"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOF97123.1"
                     /translation="MVSAHLRGSTPLLAALMFCLLLALAAPVTAGATTELHIKKLASD
                     GVSIIDEKTVDYRWLEANLPIQGDGRTHYYHQGPIFELAWEEKHPGEPYNFWNPEEDI
                     NILDKDMGAVKGTDLKDICELVGGMGEEDTVRVRATDGMSKMFPYRNVYEPESRQGPM
                     VITWYRTEDGYVPNYYTGMRLVFFADTSTNPYGIHAFGAWDMHECFDERYWYYFQPNL
                     PTTTGLSVQYVSDIIIYSEEEPVGSIYVNSTPPGATVYIDDEKAGNVTPCNIPGIEVG
                     TYPVRVELEGFAQSDEKWVTVRANTVTEVEFHLTPETGKIDVSSVPDDARIYLDGIDT
                     GKRTDTTLEDISVGKHTILLVKPDYRNATQTVTVEMDTTTKVRFDLTPENKSSGDDDS
                     SASGVENGSITITSVPANASIYLDGNRTGLFTDATLKDLPPGEHTIKLVLPGYLNMTR
                     TVTVEAQKRSFLDLIFESATPGKTVAGTGRVAVSSVPANASIYLDGNATGVRTNATLE
                     AVPAGRHTIGLMMPGYRNATKTIIVEESEHIALDLVLSPTGEEAGADYYDEPISGYTA
                     PEDTIEDPIRFPTGEKAGANTTARDEPTSGRTTPANPIEGFIGAVLGFLGNLFSIFGI
                     GGSTTPPPEPEITQVPVEHVTTPVPTDTPVPPVQEVPSVKNHSGGLYVDSYPPGMRIV
                     VDNEKLVRKTPGVIYGLREGLHTVTVEEGDPDRKTEDTGYRFEAVQVWVYPDTIAPAF
                     LDSLTTARKRTIRIDSNTCRGERFTVNGLFPAGTIPGNAGVESVRSWITVLWNGTYIS
                     YGVPYSIEEGGTFTVEPEEGGTVPVSVDSSPPGAAVFIDGFSTGMVTPCQVDGLSFGQ
                     HRIMISKVGYIPAEKVIRIPEGAGTGDTHTFALREYVSGDLLVESTVPDAKIYLYGRY
                     TGEKAPHTFSGMSIGTYEVRAVSENASRTVEDVLVTPGETTLCRVVLEE"
     gene            4835..>5958
                     /locus_tag="PLV90_08145"
     CDS             4835..>5958
                     /locus_tag="PLV90_08145"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOF97124.1"
                     /translation="MNRVRRYQTIRAAAVMLLALLLVPAASATIIWGPYVTNTTTGSA
                     TIAWKATGEEPGWVEYSSEGGEVCRVASAGEEGMHRVHLAGLLPATTYRYRVGTGGAT
                     VEDCRFRTFGDRTFTCIIYGDTQGQRPFFTQTERHGLVAERMAGEEDILFVIHTGDLV
                     GDESEWDEFFALAGPVLGNTTLVPVAGNHDGTAEVFSAIFGLPPFYSFDAGSLHVTVL
                     DSNDRAWADMERQTAWLENDLASPLPQTIVAFHHPPFSSDRKHPGGDLAIRAEWCGIF
                     AQNDVDAVFSGHTHTYERYRVNGTEYVVAGCGGGPFYPLSEEKPEGYQAGREETLGYI
                     RATISPVSIGIEVVPVADVTENGGVTMHPAGAVIDTVRLNT"
BASE COUNT         1203 a         1845 c         1820 g          990 t
ORIGIN      
        1 acttctccac cacgttcaac agttcgtttg tcaagcggaa cagaaacata atcgttgccg
       61 acgagatcga cggcgcaccg cttccaaagg acggcaagac ctggccgctg aaactcgtcg
      121 gctcggccct gacatcgaac aagcagaggg tcgggagcat cgacgagatc gtgctgaccg
      181 gggtgccggt tcccgtggtg ccgccggagg gcgatgcgac gatcaccctc tctccgggct
      241 ggaacttcgt ctcgacacca aaacggcttg ctaacggggt gaacaccctt gctatcttcg
      301 atgtagtcga taccgccgcc cactcgatcc agttctacga tggtctggag cagaagtgga
      361 ggcgggtggc tccgaccgac ccattccagc cgctcgacgc cgtctgggtc tatgcaaaca
      421 cgacctgcac cgttcccctg accttcgccc ccagcgtgcc cgatctcccg cccgcaaagg
      481 agctcgggag gggctggaac gcgatcggct tcacggatac cgttccggag tcagccatgg
      541 gcacgctgct ctcactcggg gatcgctgga gcaccctgat aggcttcgat gcagatgccc
      601 agaaatacga cgattcgatc atccgcactg tcaacagtgg ccaccacagc gtaatgcgct
      661 caatgcagcc cacaagtggc tactggatct acatgactga ggaagatacg cttgcaggta
      721 tcagcgcctg aaggagatcg atcgatgaag tacggcatcg cactgatcct cctcctcggc
      781 ttcgtcaccg gtgtggcagg ggccgggggc cagatccccg ttttacctca tgagttctac
      841 ggcagcgtga cgattgcggg atctcctgca ccggcaggaa ccgcgatcac cgcgacggtc
      901 ggcgggactg agtgcggttc gattcaggta gtggatgccg ggcgatacgg ctatctcgac
      961 tggcgccttg gaggtcgtct ggacgtgatg gcaaccgcag gacaggcggg agagacgatc
     1021 gccttctcca tcaatggggt ggccgcacag gagaccgcga ccttcatgcc cggaaaggtt
     1081 acggccctgg acctgagtgt tcccgggacc ggcggtagtg acgacaacag cagtggtgac
     1141 gacaacagcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gtgatagcgg
     1261 cgccggtagc gagagtggcg gagggagatc ccccggagat gcggggtcgc agtttgcgca
     1321 cgcccccgct gcccctgagg cacagaccgg gcgcgcatcc cttgccacgt caccggccgg
     1381 tgtggtgctc gagtccgtga cggtcaggac cgtcgacgag aactgcgccg tggcgatacg
     1441 ggagaagacc accgcacgtg acgcggccgg caacccgctc aacgaggtga ccgtggcccg
     1501 cactgccgat gttccggcag ctccgccggg aacgacggtt gctatcgccc ttgactgcgg
     1561 ccccgcgggc gccacgttcg acccgccggc cctcgtcacc tacaccctct ctgcggagga
     1621 gtgggcgaag attgacaggg gtgccacgcc gaaggtgatg tggtacaacc ccggatccgg
     1681 ggcgtggcag gatatccccg ccacggtgaa cctcgccacg cggacggtca ctgtggaagt
     1741 ctcgcacttc agcatatacg ccctggtatg gaccgtcccg gagaaggggt tcgcaggagc
     1801 agaggggacc ccggccggct ctccggtcgg agaaaccggc cctgcgttcc cggtcccggc
     1861 gctggttctc gttgtcgtgc tgatcgccgc gcttgcagca ttcctcgtta tgaggaagaa
     1921 ataacccctc ttctctgaga tggaccatgg tatcagcgca tctgcggggc tccaccccgc
     1981 ttcttgccgc cctcatgttc tgcctgctcc ttgcgctggc agccccggtt accgcaggcg
     2041 cgacgaccga gctgcacatc aaaaagctgg catcggatgg ggttagcatc atagatgaaa
     2101 agacagtcga ctaccgctgg cttgaagcaa acctcccgat ccagggtgac ggcagaaccc
     2161 actactatca ccaggggccg atctttgaat tggcctggga agaaaagcat cccggcgagc
     2221 cctacaattt ctggaacccc gaggaggata tcaacatcct tgataaggat atgggcgcgg
     2281 tgaaggggac agacctcaaa gatatatgcg aacttgtcgg cgggatgggc gaggaggata
     2341 ccgtcagggt ccgggccaca gatgggatgt caaagatgtt tccgtatcgg aacgtctacg
     2401 aaccggaatc acgccagggg ccgatggtca tcacctggta ccggactgaa gacgggtacg
     2461 tccccaacta ttacaccggg atgcgcctcg tcttcttcgc ggatacatcg accaaccctt
     2521 acggcatcca cgccttcggc gcctgggata tgcacgagtg cttcgatgag cggtactggt
     2581 actacttcca gcccaacctc cccaccacca cgggcctctc cgtgcagtac gtgagcgata
     2641 tcatcattta cagtgaggaa gagcctgtcg ggagcatcta cgtaaactca acccctcccg
     2701 gggctaccgt ctatatcgat gacgagaaag ccggcaacgt gaccccctgc aacatccccg
     2761 gcatcgaggt cggtacctat cccgtccggg tcgagcttga agggttcgcc cagtcggacg
     2821 agaagtgggt cacggtcaga gcgaataccg tcacggaggt cgagttccac cttacccccg
     2881 agaccgggaa gatcgatgta tcctcggtcc cggacgacgc acgcatctac ctcgacggga
     2941 tagatacagg gaaacggacc gacacgaccc ttgaggatat ctccgtcggc aagcatacga
     3001 ttctgctcgt gaagccggac taccgaaacg ccacccagac cgtcaccgtc gagatggata
     3061 ctaccacgaa ggtcaggttc gacctgactc ccgagaataa atcgagcggc gacgacgata
     3121 gttctgccag tggcgtcgag aacgggagca tcaccatcac atcggtcccg gcgaacgcga
     3181 gcatctacct cgatgggaat aggacagggc tcttcaccga tgcaaccctg aaggatctcc
     3241 cgcccggcga gcatacgatc aaactcgttc tgcccgggta tttgaatatg acccgaaccg
     3301 tcaccgtcga ggcacagaag cgcagcttcc tcgatctgat cttcgagagc gccactccag
     3361 gtaagaccgt cgccgggacc gggagggtcg ccgtatcctc ggtgccggca aacgcgagca
     3421 tctaccttga tgggaacgcg acgggggtac ggaccaatgc gaccctggaa gcggtcccgg
     3481 ccggcagaca tacgatcggg ctcatgatgc ccggttaccg gaatgccaca aagaccatca
     3541 tcgtcgaaga aagcgagcat atcgcccttg acctggtcct ctccccgaca ggcgaggagg
     3601 ctggagcaga ctactatgat gaacccatat ccgggtacac ggcaccggaa gacaccatag
     3661 aggatccgat ccgcttcccg acaggcgaga aggccggagc aaacaccacg gcccgtgatg
     3721 aacccacatc cgggcgaacg acgccggcta accctataga gggtttcatc ggggccgtcc
     3781 tcggtttcct gggcaatctc ttctcgatct tcgggatagg ggggagtaca acaccaccgc
     3841 cggaaccgga gatcacgcag gtccccgtcg agcatgtaac gactcccgtg ccgaccgaca
     3901 ccccggtgcc cccggtacag gaagtgccga gcgtgaagaa ccattccggc gggctctacg
     3961 tcgattccta tccaccgggc atgaggatcg tcgttgataa cgagaagctg gtgcggaaga
     4021 cgcccggggt catatacggc ctccgtgaag gcctgcacac cgtcaccgtc gaggagggcg
     4081 atcctgacag gaagacagaa gataccggct atcggtttga ggccgtccag gtatgggtct
     4141 atcccgatac gatcgcaccg gcgttcctcg atagtctgac gaccgcgcgc aaaaggacga
     4201 tccgaatcga ttcaaacacc tgccgtggcg agaggttcac ggtgaacggg ctcttcccgg
     4261 ccggcaccat accgggcaat gccggggtcg agagcgtaag gtcctggatc accgtcctct
     4321 ggaacgggac ctacatctca tacggggttc cctacagcat cgaggagggc gggacgttca
     4381 cggtcgagcc cgaagaggga ggcaccgtcc ccgtgagcgt cgactcaagc cccccgggtg
     4441 ccgcggtctt catcgacggg ttttccaccg ggatggttac gccgtgccag gtggatgggc
     4501 tctcattcgg ccagcaccgc atcatgatct cgaaagtcgg ctacatacct gccgagaaag
     4561 ttatcaggat accggaaggg gccggcaccg gcgacaccca caccttcgcg ctccgggagt
     4621 acgtcagcgg cgacctcctc gtcgagagca cggtcccgga cgcgaagatc tacctctacg
     4681 gccggtacac aggcgagaag gcgccgcaca ccttctccgg gatgagcatc ggcacctatg
     4741 aggtccgggc cgtctccgag aacgcctcga ggaccgtcga ggacgtgctg gtgacgccag
     4801 gagagacgac cctatgccgg gtggtcctcg aggagtgaac agggtgaggc gctaccagac
     4861 gatccgggca gcggcggtca tgctcctcgc tctcctcctc gtcccggcgg catcggcgac
     4921 catcatatgg ggaccgtatg tcacgaacac caccaccggg tcggcgacga tcgcctggaa
     4981 ggcgaccggc gaggagcccg gatgggtcga gtactcctcc gagggcggcg aagtttgccg
     5041 cgtggcctcc gccggggagg agggcatgca ccgcgtgcac ctcgccgggc tcctcccggc
     5101 gaccacctac cgctaccggg tcggcaccgg cggtgcaacg gtagaggact gcaggttccg
     5161 gacgttcggg gaccggacgt tcacctgcat catatacggc gacacccagg ggcagagacc
     5221 cttcttcacc cagaccgaac ggcacggcct cgtggcggag aggatggccg gagaggagga
     5281 tatcctcttt gtcatccata ccggcgatct cgtcggcgac gagtcggagt gggacgagtt
     5341 cttcgccctg gcagggccgg tgctcgggaa cacgaccctg gtgccggtgg cgggcaacca
     5401 cgacggaacg gcggaggtct tctccgctat attcggcctt ccgcccttct actcctttga
     5461 cgccggcagc ctccacgtga ccgtcctcga cagcaacgac cgggcatggg cggatatgga
     5521 gaggcagacc gcatggttgg aaaatgacct cgcctctcct ctgccccaga cgatcgtggc
     5581 gttccaccac ccgccgttca gttccgaccg gaaacacccc ggcggcgatc tggccatcag
     5641 ggccgagtgg tgcggcatat tcgcgcaaaa cgatgtcgac gcggtcttta gcggccacac
     5701 ccacacctac gagcgctacc gggtgaacgg gacggagtac gttgtcgccg ggtgcggcgg
     5761 cgggccgttc tacccgctta gcgaggagaa gcccgaaggc taccaggcgg gccgcgaaga
     5821 gacgctcggc tacatccggg caaccatcag cccggtatcg atcggcatcg aggtggtgcc
     5881 ggtcgcagat gtcacggaga atggcggggt gacgatgcat ccggcagggg ccgtcatcga
     5941 taccgtccgc ctcaatac
//
LOCUS       DAOSII010000109         5858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_12320_length_5858_cov_47.597622, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000109 DAOSII010000000
VERSION     DAOSII010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5858
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12320_length_5858_cov_47.597622"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1607)
                     /locus_tag="PLV90_08150"
     CDS             complement(<1..1607)
                     /locus_tag="PLV90_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HOF97125.1"
                     /translation="MAGEFIVPVLAWLAVIKALHLGTWPALDRTLGSLAAAAAYPASV
                     LLFTLLSWYCGILGLPILLALLPFLAGIALAGSRGFYTKERLRSTFAWDLAFLVPFLF
                     MLEVRWINPTISYAEKFMDHAFIASIMRQPIVPPLDPWYAGGTLDVYYYIGYWMNGML
                     GLASGVPSTVTFNLALPTVLGLAVVSLYALGHLLLDRHRWLPVLALLIPNPSAIYHIV
                     IGNSWFDVLWGSTRTITDTINEYPIFSMLWGDVHPHVISIFNQGFLLFLLVFAYMRWG
                     TLTTRGRVILCGLAALSLGSMPGINSWDVLIYAPVTLIFGLLVWRRYGGSALDDERSW
                     MMLAAVPPLAVAAYLPFYLQLGSSGIGGIGIVPAPSDPVEFLLVHGFFLAILVASSAP
                     DIRRRPYLLAAAVPFLLAGYPAAAIAVVPLAYLIARRRFTAADTLAILGLGIILATEI
                     IYLKDNMGETYFRMNTIFKFYLPAWLLMGTASLAIVGEWLGNAGIDGRMPARVERVLP
                     ALAVVALLATPFAINIDFGYGSHTLDGA"
     gene            complement(1597..2568)
                     /locus_tag="PLV90_08155"
     CDS             complement(1597..2568)
                     /locus_tag="PLV90_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOF97126.1"
                     /translation="MWKKVSTIVIPTLVAVGILAYMLYSVWDNLLLTLEHAVIPFLFA
                     AIGICVIAWILRGFRYQFILEGLGVPESLRFATACIFISQTANLIVPARLGDFVRMLI
                     LRHEDNATYSQGFSSIVVERVFDILMVAVLGAIALPFVLAVPDWFITVIVVPIAAGGV
                     FFIVLLWSGRMKSENRFIVAIQRMLDEVKQASLNPRALLMLSTSSLLIWLVDVLVCAA
                     VVLMFQEPVPFAIVVLAIVIGNLVKAVPITPGGVGTYELALALTFGLAGTPAATATLI
                     AVIDHLIKNMVTLVGGIGSIYYFGDWSMDLLKKVFSREIEKEETFGG"
     gene            complement(2552..3271)
                     /locus_tag="PLV90_08160"
     CDS             complement(2552..3271)
                     /locus_tag="PLV90_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOF97127.1"
                     /translation="MRDIEVSAVLPVYNDLSALKVAIPRSLETLEAIAPGGFEVIVAE
                     DGSTDGSTEFVRAYEADDPRVRLLHADERLGRGRALNRAFAAAKGSIVCYYDVDLATD
                     MQHLPELVGAIRDGCDVATGSRLLAESTITRSGGREIASRGYNMLVRTILGSRLHDHQ
                     CGFKAFRRDRLLPLLPSVAAGHWFWDTEVLVRAQKNGYRIREFPVRWQQGEGTTVRKK
                     DVVEMGSAVVRLWWQLHVEEG"
     gene            complement(3264..4496)
                     /locus_tag="PLV90_08165"
     CDS             complement(3264..4496)
                     /locus_tag="PLV90_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOF97128.1"
                     /translation="MKVCIVGGGLCGLVSALELAETHQVDLFERRPVPGGCLGSYRIG
                     DYWIEEYYHHCFSGDTSLLNLFDTLQVADRLVWLKGTTGYYVDGTIHPLTSPAEILRY
                     PHLTLIEKARLGLLTLRSRQLDAPALDGITAKEFILDNLGPGIYASFFEPLLKSKFGD
                     RREEVSAAWLISRIAIRSDRGAGGERLGYLRGGFRHFVARLEEEAANRGASIMPDSPV
                     REIRREGDGWLVDGEVYDCVVSTLPPQVTADLAGLDIAPVPYQGAACMTLALDRDVTD
                     GIYWLNMKDAAPYGAVVSHTNFAPLEWYGEHIVYLASYFSGRLPEGLEQSMLADFCRR
                     FFVSPDEVHWHRLAVDPHAGPVYTTGLRSRLPAYEEHGLFLAGMFSPPNYPERSMNGS
                     VVAGKEAAKRVLARYPDA"
     gene            4611..5777
                     /locus_tag="PLV90_08170"
     CDS             4611..5777
                     /locus_tag="PLV90_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin/FtsZ family protein"
                     /protein_id="HOF97129.1"
                     /translation="MRVFFIGFGQAGGKVVDMFIEQDKRMQTQSFRGIAVNTARTDLM
                     GLKYIELKDRLLIGQTVVKGHGVGTDNVTGARVTADEIDSIINAIDSRGTHDVDAFVI
                     IAGLGGGTGSGGSPVLARHLKRIYREPVYAIGILPAPEEGRLYSYNAARSLSTLVNEA
                     DNTFIFDNSAWKNEGESVKDAYNRLNDEIVRRFGVLFRAGEVGKAGVGEMVVDSSEVI
                     NTLRGGGISTVGYAISEKVNPRAKQSKGLLSGLMRKKEKTEEVLTGEDKSAKIIALVR
                     RAMLGRLTLPCDYSTAERGLVLLAGPPDEMDRKGVEKSKSWVEENIAGVEVRGGDYPV
                     QSDYIAAVVVLATIGNAPRITELLEIAKATKEDVLKSKEKRTTMFDDGIEPLFE"
BASE COUNT         1226 a         1703 c         1992 g          937 t
ORIGIN      
        1 gccccgtcga gggtgtggct cccgtaaccg aagtcgatgt tgatcgcgaa gggtgtagca
       61 aggagagcga cgacggcaag ggccggcagc accctctcca cccgggcagg catgcgcccg
      121 tcgataccgg cattcccgag ccactccccg acgatcgcaa ggcttgccgt tcccatgagg
      181 agccaggcag ggaggtagaa cttgaagatc gtgttcatcc ggaagtaggt ctcccccatg
      241 ttgtccttga gatagatgat ctcggtcgcg aggatgattc cgagcccgag tatggcgagc
      301 gtatcggcag ccgtaaagcg gcggcgcgcg atgaggtacg ccagcgggac gacggcgatg
      361 gctgcggcag gatagccggc gagcaggaac ggcaccgcgg cggcgaggag gtaggggcgt
      421 ctcctgatat ccggggcaga tgaggcgacg aggatcgcga ggaagaaccc gtggaccagc
      481 aggaactcta ccggatctga aggtgccgga acgattccga ttcccccgat gccggaactg
      541 ccaagctgca ggtagaacgg aaggtaggcc gcgaccgcga gcggcggcac ggcagcaagc
      601 atcatccagg accgttcatc gtcaagcgcg gagcccccgt aacgtcgcca tacgaggagg
      661 ccgaagatca gcgtgaccgg ggcgtagatg agcacgtccc aggagttgat cccgggcatg
      721 gacccgaggg agagggcggc aagcccgcag aggataaccc ggccccgcgt ggtgagcgtc
      781 ccccaccgca tgtaggcaaa gaccaggagg aagagcagga acccctggtt gaagatgctg
      841 atgacgtgcg ggtgcacgtc gccccagagc atcgagaaga tggggtactc gttgatggta
      901 tccgtgatcg tccgggtgct cccccagagg acgtcgaacc acgagttccc gatgacgata
      961 tggtagatcg ccgaggggtt cggtatgaga agcgcaagca cggggagcca ccggtggcgg
     1021 tcgaggagga ggtgcccgag ggcgtagagc gagacgacgg caagcccgag caccgtcggg
     1081 agggcgaggt tgaacgtgac cgtcgaggga acgccggatg caagcccgag catcccgttc
     1141 atccagtagc cgatgtagta gtagacgtcc agcgtccctc ccgcatacca gggatcgagc
     1201 ggcgggacga taggttgccg catgatcgaa gcgatgaacg cgtggtccat gaacttctcg
     1261 gcgtaggaga tggtcgggtt gatccagcgc acctcgagca tgaagaggaa agggaccagg
     1321 aaggcgagat cccaggcgaa tgtcgagcgc aggcgctcct tcgtgtagaa ccctcgcgac
     1381 ccggcaaggg cgatgccggc aaggaacggg aggagggcga gcaatatagg aaggcccagg
     1441 atgccgcagt accaggagag gagcgtgaag agaagaaccg atgccgggta tgcggcggcg
     1501 gccgcgaggc tgccgagggt gcggtcgagc gccggccagg tgccgaggtg gagcgctttg
     1561 atgaccgcaa gccaggcgag caccgggacg atgaactcac ccgccaaacg tctcctcctt
     1621 ctcgatctcc cgggaaaaga ccttctttaa gaggtccatc gaccagtcgc cgaagtagta
     1681 gatcgacccg atgcccccga cgagcgtcac catgttcttg atgaggtgat cgatgaccgc
     1741 gatcagcgtc gccgtggccg ccggcgttcc cgcgagcccg aaggtcagtg caagcgcgag
     1801 ctcgtaggtc cccacgccgc cgggggtgat cggcaccgcc ttgacgaggt tgccgatgac
     1861 gatagcgagg acgacgatgg cgaacgggac cggttcctgg aacatcagga cgaccgcggc
     1921 gcagaccagc acatcgacga gccatatgag gagcgatgag gtgctgagca tgaggagggc
     1981 ccgggggttg agggaggcct gtttcacctc gtcaagcatc ctctggatgg cgacgatgaa
     2041 ccggttctca gacttcatcc tgcccgacca gaggagcacg atgaagaaga cgcctcccgc
     2101 ggcgatgggg acgacgatga ccgtgatgaa ccagtcgggg acggcgagga cgaacgggag
     2161 cgctatggcg ccgaggaccg cgaccatcag gatatcgaag acgcgctcga cgacgatgga
     2221 cgagaacccc tgcgagtagg tggcgttatc ctcgtgcctg aggataagca tccgcacgaa
     2281 gtcgccgagg cgtgcgggaa cgatgaggtt tgccgtctgg gatatgaata tgcaggcggt
     2341 cgcaaacctg aggctttccg ggacgccgag tccctccagg atgaactggt atcgaaaccc
     2401 ccggaggatc caggcgataa cgcagatacc gatggcggca aagaggaagg gtatcactgc
     2461 atgctcgagc gtcagcagga gattgtccca tacgctgtaa agcatgtagg cgaggatccc
     2521 gaccgcgacc agggtcggga tgacgatcgt gctaaccttc ttccacatgg agttgccacc
     2581 agaggcgcac gaccgccgag cccatctcga cgacgtcttt tttgcgcacg gtcgtcccct
     2641 ctccctgctg ccaccgcacg ggaaactccc gtatacggta gccgttcttc tgtgcccgca
     2701 ccagcacctc ggtgtcccag aaccaatggc ccgcggcgac cgacgggagc aggggaagaa
     2761 ggcggtcgcg ccgaaacgcc ttgaacccgc actggtggtc gtggagtctg ctcccgagga
     2821 tcgtccggac aagcatattg tagccgcggc tcgcgatctc ccgtcccccg ctccgggtga
     2881 tggtgctctc cgcaaggagg cgggatccgg tcgcgacgtc gcagccgtcc cggatggcgc
     2941 cgaccagttc ggggaggtgc tgcatatcgg tggcgagatc gacgtcgtag tagcagacga
     3001 tcgatccttt cgccgcggca aacgcgcggt tgagcgcccg gccccgcccg agacgctcgt
     3061 cggcatggag caggcggacc cggggatcgt ctgcctcata cgccctgacg aactccgtgc
     3121 tcccgtcggt gctcccatct tcggcgacga tcacctcaaa cccccccggc gcgatcgcct
     3181 caagcgtctc aagcgaccgc ggtatggcga ccttgagcgc cgatagatcg ttatagaccg
     3241 gcaggacggc gctcacctcg atatcacgca tcggggtacc tcgcaagaac ccgctttgcc
     3301 gcttccttgc cggcgacgac cgaaccgttc atgctccgct cgggatagtt cggggggctg
     3361 aacataccgg cgaggaagag cccgtgctcc tcgtaggcgg gcaggcggct ccggagcccg
     3421 gtcgtgtaga ccggacccgc gtgggggtcg accgcgagcc ggtgccagtg cacttcgtcc
     3481 gggctgacaa agaaccgcct gcagaagtcc gcgagcatcg actgctcgag tccctccggg
     3541 agccggccgg agaagtaaga cgcgaggtag acgatatgct cgccgtacca ctcgagcggg
     3601 gcgaagttgg tgtgcgagac caccgcaccg tatggggcgg catccttcat gttcagccag
     3661 tagatgccgt cggtcacgtc ccggtcgagt gcgagggtca tgcaggcggc gccctggtag
     3721 ggcaccgggg cgatatcgag gccggcaagg tccgcagtca cctgcggggg gagcgtcgac
     3781 acgacgcagt cgtagacctc gccgtcgacg agccagccgt cgccctcccg ccggatctcc
     3841 ctgaccgggg agtcgggcat gatcgaggcg ccccggtttg ccgcctcttc ttcgagccgc
     3901 gcgacgaagt gccggaaccc tccccggagg tagcccagac gctcgccgcc cgcaccccgg
     3961 tcggaccgga tcgcgatgcg ggatatgagc caggctgcgg agacctcttc gcggcggtcc
     4021 ccgaacttgc tcttcaagag cggttcgaag aacgaggcgt agatgccggg cccgaggtta
     4081 tcgagaatga actcctttgc ggtgatcccg tcgagggccg gagcgtcgag ctgccgggag
     4141 cgcagggtca gcaggccgag gcgggccttc tcgatgagcg tgagatgggg atagcgcagg
     4201 atctcggcag gagaggtgag cgggtggata gtcccgtcga cgtagtagcc ggtcgtgccc
     4261 ttgagccata ccagccggtc ggctacctga agggtgtcga acagattgag caggctggtg
     4321 tcgcccgaga agcaatggtg gtaatattcc tcgatccagt aatcgccgat ccggtatgaa
     4381 ccaagacagc ctcccggaac cggccgtcgt tcgaagaggt ctacctggtg tgtttcggca
     4441 agttcgagag ccgagaccag accgcataac cctcccccca caatgcagac cttcatcgct
     4501 taccactacg tgtgaagtta taagaacact attgggctat aagagtatac aacgtcttta
     4561 tacataaacg tcgatattat tgattaggct atatgagggt gtgaagctga atgagggtct
     4621 tcttcatagg attcggtcag gcggggggca aagtcgtcga tatgttcatc gagcaggata
     4681 aacggatgca gacccagagt ttcaggggta tcgcggtgaa cacggcgcgt accgacctga
     4741 tggggctcaa atatatcgaa cttaaagacc ggctcctgat cggacagaca gtagtcaagg
     4801 gtcacggtgt cgggaccgac aacgtgaccg gagcacgggt gaccgccgat gagatcgaca
     4861 gcatcatcaa cgccatcgac tcgagaggca cgcacgacgt cgatgctttc gtgatcatcg
     4921 ccggtctcgg gggcgggacg ggctcggggg gctcgccggt actcgcccgg cacctgaagc
     4981 gcatctaccg cgaaccggtc tatgcgatcg gtatcctgcc cgcccccgag gaaggccggc
     5041 tctattcata caatgcagcc cgttccctga gcaccctggt gaacgaagcg gataatacgt
     5101 ttatcttcga taacagcgcc tggaagaacg agggggagag cgtcaaggac gcctacaatc
     5161 ggttgaacga cgagatcgtc cgccggttcg gcgtgctctt ccgcgcaggc gaggtcggca
     5221 aggccggcgt cggcgagatg gtcgtggact caagcgaggt catcaacacc ctgcggggcg
     5281 gcggcatcag caccgtcgga tacgccatca gcgagaaggt caacccccgc gcgaagcaga
     5341 gcaaggggct cctctccggc ctcatgcgga agaaggagaa gaccgaggag gttctgaccg
     5401 gagaggacaa gtcggcaaag atcatcgctc tcgtcaggcg ggcgatgctc gggcgcctta
     5461 ccctgccgtg cgactactcg acggcggagc ggggtcttgt cctcctcgcg ggtccgccgg
     5521 acgagatgga caggaagggc gtcgagaagt cgaagagctg ggtggaggag aacatcgccg
     5581 gcgtcgaggt gcgtggcggc gactacccgg tgcagagcga ctacatcgcc gcggtggtgg
     5641 ttcttgcgac gataggaaac gccccccgga tcaccgaact ccttgagatt gcaaaggcta
     5701 cgaaagagga tgtcctgaag tcgaaagaga agcgtacgac catgttcgac gacggcatag
     5761 aaccattgtt tgagtgagga ggtagtatga aggcaaacat cagcagatgg atcattctcc
     5821 tgctggcact cgtctgtgtc gtgcaggcgg tatcggcg
//
LOCUS       DAOSII010000110         5701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_12736_length_5701_cov_47.889834, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000110 DAOSII010000000
VERSION     DAOSII010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5701
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12736_length_5701_cov_47.889834"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="PLV90_08175"
     CDS             <1..515
                     /locus_tag="PLV90_08175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOF97130.1"
                     /translation="DKVALAIKSMNGTYDLKFLIVGDGDTKKHLEQLVKTENLADSII
                     FTGTLPREEIPRLLSESLVGMAPLKRLANLEYAAPTKAYEYMACGLPFVGCGDGEIAH
                     LAHESGAGVIAGNTPEAIAATLATLLDDPGRMEEMGRRGREYVAEHYGRRAVALKLKQ
                     HIERMTWTSA"
     gene            500..1687
                     /locus_tag="PLV90_08180"
     CDS             500..1687
                     /locus_tag="PLV90_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97131.1"
                     /translation="MDERLTLLLSRVAGKVRELWVTDGETAYIRDPVFPVIRNRVHAE
                     FCKAMLRSGGDTLVGPILNYVTACQNPDGSWNEVHVNYNEPSALITAFIGDALIEAAD
                     RHPHEDALERARDFVLAAERSPGCFLKSAGYTADHLNVDASCGAFLARYAERYDDREA
                     RAAASRAAENVVSHQRDGVYPYAVDKGTYPYVFDLPCIHYQGVTIYYLAKTNDVLCDD
                     RIDRSIAEAARWLAAAQRPDGRFDWSGSGLSFACYLSGAYAFAQASFTYASRLDDRYR
                     EHADRSLDWLDANVRGLAPRWEPASWGDLPLSVAAAVKSASLGGYPLRHRAFRFGYGV
                     YREVARRRYSATAETRAFEALSGLLGIRSSTVEPSKNFPDLFMTSEMLDCLSQSMIWG
                     KNT"
     gene            1684..3825
                     /locus_tag="PLV90_08185"
     CDS             1684..3825
                     /locus_tag="PLV90_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HOF97132.1"
                     /translation="MKQVLLDLQSGSIRVEDVPVPAVTRGVVVENAYSLISAGTESSL
                     INLAGESLIGKARARPDDVKKVLQKIGTDGPLPAYRQAMSRLAKPEPLGYSSAGTVVK
                     TGTDDFEVGDRVACAGAGYAVHAEYVAVPKNLCVRIPDGVGLREAAFTTVGSIAMHGV
                     RNAHVTVGENVAVIGLGLIGLLTVQILKAAGCRAIGIDIDREKLALAADLGADAVSNY
                     DGLAERMKVFSPFGADAVVITAATPSSAPIEAAGRLVRDKGRVVVVGNVGMNVPRDIF
                     YEKEAEVVVSRSYGPGRYDRNYEERGVDYPIYVRWTERRNMEAFLELVRQKRIDLDRL
                     ITHTFPLDSAPEAYDLISAGRERYIGILLQYSPNGAGASGTVTRLSGPVARKVPAGAR
                     TIGCIGAGVHAQSALYPNLPGLPVNLAGVATATGLSAQSVAKKYGFAYCTTDYRAILD
                     DPGIDAVLIATRNDLHAPMAIDALAAGKDVFVEKPLATDTEGLRGVVRAYSESENRLM
                     VGFNRRYSPLARKMKEFFEKRSTPMVLHYRVNAGQIPPGHWVHDDEQGGGMLISECCH
                     FFDLMQYLTGARPVQVYARAIEATGTLRKYDNFQATLAFADGSVGTVTYTTLGDRSYS
                     KETVEVFCDNAVGRITDFRDLELRRDGKVARERRWLAQEKGFIEELRAFVKGEEADFA
                     GSVATTLATFRAWESIDTGRSQKIDIGTVGL"
     assembly_gap    3844..3943
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3947..5041)
                     /locus_tag="PLV90_08190"
     CDS             complement(3947..5041)
                     /locus_tag="PLV90_08190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosine biosynthesis radical SAM protein
                     RaSEA"
                     /protein_id="HOF97133.1"
                     /translation="MAGDLRRQNGSIPDNLDFTVPIGWEERIGHLGDRPIKRLIVFLR
                     STGCEWVEKTGGCTMCGFYCATSRGRAISAEEYVAQFEYVMDAVDLGDYPVVSIYNDG
                     NIFNEREMPTAAIEKICSYIDGYKNIRKVVVESRIDYSPDEHVQKMKKALGGRQLEVA
                     FGFESADPQVMNLCINKGFSAHNFDHFHSRMRSMGVLTKPLLLVKPPFLTDAESIDDV
                     LKTVAYCVSRGIEYVDLEVTTVEKNTVVHELWKNNLYRPPWLWSLVDLLQQCRERFGD
                     RTHVYVSPWTYSVESLDWARNCGECDAGFVKAIERYNRHFDTAEFEGLDCSCRDDGWQ
                     EAAEIKDPRSIPERIRAQLAYVRNARLSSE"
BASE COUNT         1032 a         1741 c         1809 g         1019 t
ORIGIN      
        1 tcgataaggt ggccctcgcg atcaagtcga tgaacggcac ctacgacctg aaatttctca
       61 tcgtcggcga cggcgacaca aagaagcacc tcgaacagct ggtaaagacc gagaacctgg
      121 ccgactcgat catcttcacc ggcaccctcc cgcgcgagga gatcccgcga ctgctctccg
      181 agtcgctcgt ggggatggcg cccctgaaga gacttgcaaa cctcgagtac gccgctccca
      241 ccaaggctta cgaatacatg gcctgcgggc tgcccttcgt cggctgcggg gacggcgaga
      301 tcgcccatct cgcgcatgag tcgggcgccg gggtcatcgc cggcaacacg ccggaggcga
      361 tcgccgcgac gcttgccacg cttcttgacg acccggggag gatggaggag atgggccgcc
      421 gggggcggga gtacgttgcc gagcactatg gccggagagc ggtcgccctg aaactgaagc
      481 agcatatcga gaggatgaca tggacgagcg cctgacgttg ctcctctccc gggttgccgg
      541 gaaggtccgg gagctttggg tcaccgatgg agagaccgcg tacatccgcg acccggtctt
      601 tcccgtcatc cggaaccgcg tgcacgccga gttctgtaag gcgatgctcc gttccggcgg
      661 cgacacgctc gtaggcccga tcttgaacta cgtcacggcc tgccagaacc cggacgggtc
      721 ctggaacgag gtccacgtca actacaacga gccttcggcc ctgatcaccg ccttcatcgg
      781 cgatgcgctc atcgaggcgg cggatcggca tcctcacgag gatgcgctcg agcgggcccg
      841 ggacttcgtc ctcgcagccg agaggagtcc cggctgtttc ctgaaatctg caggatatac
      901 agccgaccac ctgaacgtcg acgcctcctg cggggcgttt cttgcccggt acgcggagcg
      961 ctacgacgac cgggaggcac gcgcggccgc cagtcgtgcg gcggagaacg tcgtctccca
     1021 ccagagggac ggcgtttatc cctatgccgt cgataaggga acctaccctt acgtcttcga
     1081 cctgccctgc atccactacc agggagtgac gatctactac ctggcaaaga cgaacgacgt
     1141 gctctgcgac gaccggatcg accggagcat cgcggaggcg gcccggtggc tcgcggccgc
     1201 ccagcgcccc gacggccggt tcgactggtc gggaagcggg ctctcctttg cctgctacct
     1261 cagcggggcg tacgcgttcg cccaggcctc gttcacctac gcatcccgcc tcgatgaccg
     1321 ctaccgggag cacgccgatc gctctctcga ctggctcgat gcgaacgtca ggggtcttgc
     1381 gccccgttgg gaaccggctt cctggggcga tctccccctc tcggtcgcgg cggcggtaaa
     1441 atccgcgtcg ctcgggggat acccgctccg gcaccgggcg ttccggttcg gctacggtgt
     1501 ctaccgggag gtcgcccgcc ggcggtatag cgcgaccgcg gagacaagag cattcgaggc
     1561 gctctcgggg ctgctcggta tcaggtcgtc gaccgtggag ccgtcgaaga actttcccga
     1621 cctcttcatg acatcggaga tgctcgattg cctcagtcaa tcgatgatat ggggaaaaaa
     1681 cacatgaaac aggtattact cgatctgcaa tcagggtcca tccgggtgga ggacgttccc
     1741 gttcccgccg taacgagagg ggtcgtcgtc gagaacgcct actccctcat cagcgccggc
     1801 acggagtcgt cgctgatcaa ccttgcggga gagtcgctca tcggcaaggc gagggcacgt
     1861 cccgacgacg tgaagaaggt cctccagaag atcgggacgg acggccccct cccggcatac
     1921 cggcaggcga tgagccgtct tgcaaaaccg gagccgctcg gctacagttc tgccggcacc
     1981 gtggtgaaga ccgggaccga cgacttcgag gtcggcgacc gcgtcgcctg cgccggggcc
     2041 gggtatgcgg tgcacgccga gtacgtcgcg gtcccgaaga acctctgcgt caggataccg
     2101 gacggtgtcg gtctgcggga agcggcgttc acgaccgtcg gatcgatcgc gatgcacggt
     2161 gtcagaaacg cccatgtcac ggtgggcgag aacgtcgcgg tgatagggct cgggctgatc
     2221 gggcttctca ccgtccagat cctcaaagcc gcggggtgcc gggcgatcgg gatcgatatc
     2281 gaccgggaga aactcgcgct cgcggcagac ctcggtgccg atgccgtctc gaactacgat
     2341 ggccttgcgg agaggatgaa ggtcttctcg ccgttcgggg cggatgcggt cgtcatcact
     2401 gcggccaccc cgtcaagcgc ccctatcgag gccgcgggcc gcctggtcag ggataagggc
     2461 agggtcgtcg tcgtcgggaa cgtggggatg aacgtgcccc gcgatatctt ctacgagaaa
     2521 gaggcggagg tcgtcgtctc ccggtcctac ggccccggac gctacgacag aaactacgag
     2581 gagcgggggg tagactaccc gatctacgtc cggtggacgg agcggaggaa catggaggcg
     2641 ttcctcgaac tggtgcggca gaagcggatc gacctcgacc gcctgatcac ccataccttt
     2701 ccgctcgata gcgcgccgga ggcctacgac ctgatcagtg ccgggagaga gcggtacatc
     2761 ggcatcctgc tgcagtatag cccgaacggc gcgggcgcct ccggcaccgt cacccgcctc
     2821 tccgggccgg ttgcgcggaa ggtgccggcc ggggcgcgga cgatcgggtg catcggcgcc
     2881 ggagtccacg cccagagcgc cctctacccg aacctccccg ggctcccggt gaaccttgcc
     2941 ggtgtcgcga ccgcgacggg gctctcggcg cagtcggtcg caaagaagta cgggtttgcc
     3001 tactgcacga ccgactaccg cgcgatcctc gacgatcccg ggatcgatgc ggtgctgatt
     3061 gcgaccagaa acgatctcca cgctccgatg gcgatcgatg cgcttgccgc cggcaaagac
     3121 gtcttcgtgg agaaacctct tgcaacggac accgaagggc tccggggggt ggtccgggca
     3181 tacagcgagt cggagaaccg gctgatggtc gggttcaacc ggcgctactc tccgcttgcg
     3241 cggaagatga aggagttctt cgagaagagg agcacgccga tggtcctgca ctaccgggtc
     3301 aacgccgggc agatcccgcc cgggcactgg gtgcacgacg acgagcaggg cggggggatg
     3361 ctgatatcgg agtgctgcca cttcttcgac ctcatgcagt acctgaccgg ggcacggccg
     3421 gtccaggtct acgcccgggc gatcgaggcg accggaaccc tccggaagta cgataacttc
     3481 caggcgaccc tggcgtttgc ggacgggtcg gtcggcaccg tcacctacac gacgcttggc
     3541 gaccgctcct actcgaagga gaccgtcgag gtcttctgcg acaacgccgt cgggaggatc
     3601 accgatttcc gcgacctcga actccggcgg gacgggaagg ttgcgcggga gaggcggtgg
     3661 cttgcccagg agaaggggtt catcgaggag ctccgggcat tcgtgaaagg agaggaggcg
     3721 gactttgccg gcagcgtcgc gacgacgctt gcaacgtttc gcgcgtggga gtcgatcgat
     3781 accgggcgat cgcaaaagat cgatatcggc acggtcgggt tgtgatgcgg cccggttggc
     3841 tcannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncccctac tctgaagaga
     3961 gccgggcgtt cctcacgtac gcgagttgag cgcggatcct ctccggtatc gagcgggggt
     4021 cctttatttc ggcggcttcc tgccacccat cgtcccggca cgagcagtcg agcccctcaa
     4081 actccgccgt atcgaagtgg cggttgtagc gttcgattgc tttgacgaac ccggcgtcgc
     4141 actctccaca gttcctggcc caatcgagag attcgaccga gtaggtccag gggctgacgt
     4201 agacgtgggt acgatccccg aaccgctccc ggcactgctg caggaggtcg acgaggctcc
     4261 agagccatgg cggccggtag aggttgttct tccagagctc gtgcacgacg gtgttcttct
     4321 cgaccgtcgt cacctcgaga tccacgtact cgataccgcg ggagacgcag taggccacgg
     4381 tcttgaggac gtcgtctatc gactcggcat cggtgaggaa cgggggtttg acgaggagga
     4441 gcggctttgt cagaacaccc atgctccgca tcctcgaatg gaagtggtcg aagttgtggg
     4501 ccgagaaccc cttgttgatg cagaggttca tcacctgcgg gtctgcagac tcaaacccga
     4561 acgccacctc gagttgccgc ccgccgagtg ccttcttcat cttctgcacg tgctcatcag
     4621 gcgagtaatc gatccttgac tcgacgacca cctttctgat gtttttatag ccgtcgatat
     4681 acgagcagat cttctcgatg gcggcagtcg gcatctcgcg ctcgttgaat atgtttccgt
     4741 cgttgtagat cgatacaacc ggataatccc cgagatcgac ggcgtccatc acgtactcga
     4801 actgcgcgac gtactcctcc gccgagatgg ccctgccccg ggacgtcgca cagtaaaacc
     4861 cgcacatggt gcaacccccg gtcttctcga cccattcgca acccgtggag cggaggaaga
     4921 cgatcaaccg cttgataggc cggtcgccaa gatgcccgat ccgctcctcc cagccgatgg
     4981 gaacggtaaa atcgagattg tccgggatgg acccgttctg cctgcgcaga tcgccggcca
     5041 atgcccgtat ctcgtcaagg acgtcatttt cggttcggag accggtgaag atctctgtgc
     5101 tgctactgct atcaggtacg gccttttcca gaataattat cccccctgta cccggcgtgc
     5161 gccgccatat cgggggtgca cggcccggat gactgtcgcc ttatcttacc aagacatacc
     5221 cctcataggc ttttgcgtat ttatagtttg tccaaaccgt cccgtcaccg gggtatgttt
     5281 gccaaacggt ttttcgtgat ggaaaatcgc gaggatcgga ccggccaccg gggagggtgg
     5341 gatcctggtc gttctctcct ccccccgatg ggttatgtat gtggttataa agctccaatc
     5401 tccagtcgag cccttgacag catgccaatc gttatatata tcgtccgggg gtatactacc
     5461 ccgatgcacc gggtatcgtt gcctgccggg atcggctccc ggcatcccgc cggaggatgc
     5521 tgaggagagg tgtggtgttc ctgcagggac acgggaagag cccgggaggt ttgaggagca
     5581 gtgcggcgat gccgtccggt atgggttatg gctgaagaat acgtaagaag aagggagaac
     5641 ggtgtggtgt accccggaga taaggcctgc gaccgggact cgttcacggc aagagctctt
     5701 g
//
LOCUS       DAOSII010000111         5673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_12813_length_5673_cov_50.855465, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000111 DAOSII010000000
VERSION     DAOSII010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12813_length_5673_cov_50.855465"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="PLV90_08195"
     CDS             complement(<1..121)
                     /locus_tag="PLV90_08195"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HOF97134.1"
                     /translation="MKKRPPMNRAERAQMAMMLEVSAYPKPGNVDRCHDYADTR"
     gene            complement(90..1229)
                     /locus_tag="PLV90_08200"
     CDS             complement(90..1229)
                     /locus_tag="PLV90_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 9 domain-containing
                     protein"
                     /protein_id="HOF97135.1"
                     /translation="MMEAYDRFELLINDRIVKTPVAIASMAGVVDAGYVLERAAHVGA
                     AFIGGYSIDGPTLDASRRMAAEGRKEFLYDDPLDALAKEIDALKGSDVITGVNLRGST
                     PAAYVDVARAFEDRVVYEIDAHCRQPAMLEAGCGEYLLKHPSKLTEIIRALKSANVTV
                     SVKIRAGVAADDRDLARAIWKAGADILHADLMDYGYARVRQIRNASPLMLIANNSLNT
                     FEKAMDAFSHGADLVSLARRSDPWTLAGLDAAITRYADEGGWYNAPKQLCRGGDIRAL
                     TFCCMPVKACPLIPTLEKIGLSRNEYLKLKQEAVKGTPLDDGKGTCFGGLAWCCKISS
                     PCMFRNMTLEQKGLSAQEYMRCKHRLSEKILHRLFDEEETADEPS"
     gene            1494..1901
                     /gene="cfbA"
                     /locus_tag="PLV90_08205"
     CDS             1494..1901
                     /gene="cfbA"
                     /locus_tag="PLV90_08205"
                     /EC_number="4.99.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin nickelochelatase"
                     /protein_id="HOF97136.1"
                     /translation="MSMKGMLLVGHGSKLPYNKELIETTAAIIAGKTDEYLVKPGFMS
                     INAPTVEEQLAAFRREDIDMLVVVPLFLARGIHIEQDIPRLLGLAPGVRTGEFQLNGK
                     TVPLVYANPIGSDPLLAELMLKNASDAIAELKP"
     gene            1906..3084
                     /gene="cfbE"
                     /locus_tag="PLV90_08210"
     CDS             1906..3084
                     /gene="cfbE"
                     /locus_tag="PLV90_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F430 synthase"
                     /protein_id="HOF97137.1"
                     /translation="MRILVLDTIHGGAELAVALREAGHEADEVDVYRGKAGIPVAEAL
                     ARTYDLVTAPVHLDPDHPLLRQHGYAVSHHEMVGWVLRTHPPRPFIEITGARGKTTTA
                     HALASLLPGPGILHTSTGTYRYPERKILWKKSITPASLLSAAHEAWRTGGWLVAEESL
                     GVTGAGDVAVLTSTDDYPIAAGKKSAIAEKCRLITRARTIVVPERIDLPRSAVAADGM
                     VSFEGPVCRYAGRGIAGTFTNPLCTLSGYRTAFALATATACVLGIDPAPLAEFTALSG
                     RMAARREGDRMIVDDANSGTNAATVIEAARYARELTGNGALTLVIGEEARAVCEGFSP
                     EDVRRAVSAVSPTGVVYVGEAWSAAGEEHTAPSLEAGLDAARRITPAGAIVLAVKTWR
                     "
     gene            3087..4151
                     /gene="cfbD"
                     /locus_tag="PLV90_08215"
     CDS             3087..4151
                     /gene="cfbD"
                     /locus_tag="PLV90_08215"
                     /EC_number="6.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide reductive
                     cyclase catalytic subunit"
                     /protein_id="HOF97138.1"
                     /translation="MDYIQPRPSSIVAALYTARDLGVDVAILHGPSGCSFKHARLLEE
                     DGMRVLTTSLADNEFIFGGHDPLVRVLRHAEKEFTPRRMAVVGTCVAMIIGEDLQSAL
                     HDAGTRVPAIAVDIHAGFRENIDGVLATLEPAAAAGWISGAELERQRYLLGKANEVER
                     LRGAASRHYIEPSRGDLKHVAARRLVDLADSGAIGVAVMNAKKETAYMFADELVALHD
                     TCPDAAITYIANLEDRGLPKVRNDAARILAGMRERGVDPELLGALDEYGANGDAIGER
                     LREMKPDFALMVGVPHAVPPEYTEGIEVFSITNGPRQVEPLRDMGHRHVMVEIDLHPK
                     TLGVREIVASEFGAVLRSMR"
     gene            4148..5515
                     /gene="cfbB"
                     /locus_tag="PLV90_08220"
     CDS             4148..5515
                     /gene="cfbB"
                     /locus_tag="PLV90_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide synthase"
                     /protein_id="HOF97139.1"
                     /translation="MKSFLVAGDRSGSGKTSITLALSALLSTTRAVQTFKVGMDYIDP
                     SYLTGVTGRPCRNLDGYVMSPAEVRAVFAHGCRGADVAVVEGVRGLFEGAEALTDLGS
                     TAAIAKQLDLPVVLVVNARSITRSAAAIVKGFMAFDPDVAIRGVILNQVTGARHREKA
                     VRAIEHYCGIPVVGVIPRTADMELAMRHLGLVPYREGQEHGEFLARIEAVKQMIGEHV
                     DIDALLSLAREGRAPTPEDPIYAAVDPDVRIGVALDEAFNFYYADLFDVLASSGAEVV
                     PFSPVHGRLPEADGYIIGGGSPEVFGPELEANTAVREGLREASRNGIPVYAECGGLVY
                     LTERMVLAPGFTASKREETYDLAGVFAGEARMPEKRMLGYVVGTSAGENPMGAAAFKG
                     HEFHYSSVRLAPETRYAYRLTRGSGIRDGLDGAVRDRTIGSYTHLHPVTSRGMFANFV
                     AGCRG"
BASE COUNT          969 a         1825 c         1934 g          945 t
ORIGIN      
        1 ggcgcgtgtc ggcgtagtcg tggcaccggt ccacgttccc gggtttcggg taggccgaga
       61 cctcgagcat catggccatc tgcgcccgtt cagctcggtt catcggcggt ctcttcttca
      121 tcgaacagcc tgtgcaggat cttctcggac aggcggtgct tgcaccgcat gtactcctgc
      181 gccgagaggc ctttctgctc aagcgtcatg ttccggaaca tgcaggggga actgatcttg
      241 cagcaccagg caaggccccc aaaacaggtg cccttcccgt cgtcgagcgg ggtgcccttc
      301 accgcctcct gcttgagctt gaggtactcg ttccgggaga gcccgatctt ctcgagggtc
      361 ggtatgaggg ggcaggcctt caccggcatg cagcagaagg tgagcgccct gatgtcgccg
      421 ccgcggcaga gctgtttcgg ggcgttgtac cagccgcctt cgtcggcata ccgggtgatg
      481 gcggcatcga ggccggcaag cgtccagggg tcggagcgcc gggcaagcga gacgaggtcc
      541 gccccgtgag agaaggcatc catcgccttc tcgaaggtgt tgagggagtt gttcgcgatc
      601 agcatcaggg gggaggcgtt gcggatctgg cggaccctgg cgtagccgta gtccatcagg
      661 tccgcgtgga gtatgtccgc tcccgccttc cagatagcgc gggcgaggtc gcggtcgtcg
      721 gcggcgacgc cggcccggat cttgaccgat acggtcacgt tcgccgactt caaggcgcgg
      781 atgatctcgg tgagtttcga ggggtgtttg agaaggtatt cgccgcaccc ggcctcaagc
      841 atcgccggct gccggcagtg ggcatcgatc tcgtagacca cgcggtcctc aaacgcgcgg
      901 gcgacatcga cgtacgcggc cggcgtgctc ccgcggaggt tgacgcccgt gatgacgtcg
      961 cttcccttca gggcatcgat ctctttagcc agggcgtcca gggggtcgtc gtacaggaac
     1021 tcttttcggc cctccgctgc catccgccgg cttgcatcaa gggtggggcc gtcgatggaa
     1081 tagccgccga tgaaggcggc ccccacgtga gccgccctct caagaacgta acccgcatcc
     1141 actactccgg ccatggatgc tattgccacc ggtgttttta cgatccgatc gtttatgagg
     1201 agctcaaaac gatcataagc ttccatcatg cttctctatt cgttgtcggt atgaggggat
     1261 attgatttgt atcgggtcag ggaccggtcg gcacccggcg cccgggaggc ccgtataccg
     1321 ggcgcgctcc ggcccgggcg ataacagggc cttccggcgt tccggcgtgc aaaaagccgg
     1381 cagatatcgg gggcgacgat ccatccgaag cccggcatcg ccgcgatacc gggccggacc
     1441 cttacgaaca atcagtaatt attttaatat agtatcgtag taccctgtaa catatgagta
     1501 tgaagggaat gttactggtc gggcatggca gcaagctccc ctacaacaag gaactgatcg
     1561 agaccaccgc ggcgatcatc gccgggaaga ccgacgagta tctcgtcaaa cccggtttca
     1621 tgagcatcaa cgcgccgacg gtcgaggagc agcttgcggc gttccggaga gaggatatcg
     1681 atatgctggt cgtcgtcccg ctcttcctcg cacgaggcat ccacatcgag caggatatac
     1741 ccaggctcct cggtcttgct cccggggtaa gaacaggcga attccagctg aacgggaaga
     1801 cggtgccgct cgtctacgca aaccccatcg ggagcgaccc gctccttgcc gagctgatgc
     1861 tgaagaacgc atccgatgcg atcgccgaac tgaagccctg aggctatgcg gatcctggtg
     1921 ctggatacca tccacggcgg ggcagaactt gccgtggcgc tccgggaggc cggccacgag
     1981 gcggatgagg tggatgtcta ccgcggaaag gccggcattc ctgtagcgga ggcgctcgcc
     2041 cgcacctacg acctcgtcac cgcaccggtc cacctcgacc cggaccatcc tctcctccgg
     2101 cagcacgggt acgcggtctc ccaccacgag atggtggggt gggtcctcag gacccaccct
     2161 ccccgcccgt tcatcgagat caccggtgca cggggcaaga ccaccacggc gcacgcgctc
     2221 gcttcgctgc tgccgggccc gggcatcctg cacacgtcga ccgggaccta ccgctatccg
     2281 gaacgaaaga tcctctggaa gaagagcatc acgcccgcct ccctgctgtc ggcggcgcac
     2341 gaggcctggc gcaccggagg ctggctggtc gcggaggagt cgctcggcgt cacgggggcc
     2401 ggggatgtgg cggtgctgac atcaacggac gattacccga tcgcggcggg gaagaagagc
     2461 gcgatcgccg agaaatgccg gctgatcacc cgggcgcgga cgatcgtggt cccggagagg
     2521 atcgacctcc cgcggtcggc ggtcgccgcc gacgggatgg tctccttcga gggtccggtc
     2581 tgccggtatg ccgggcgcgg gattgccggc actttcacaa acccgctctg caccctctcc
     2641 ggctaccgga cggcgtttgc gcttgcaacc gcgacggcct gcgtgcttgg catcgacccc
     2701 gcccctctcg cggagtttac cgctctttcc ggccgtatgg cggcccggcg agaaggggat
     2761 cgtatgatcg tggacgacgc caacagcgga acgaatgcgg ccaccgttat tgaagccgcc
     2821 cgttatgcgc gggaactcac cgggaacggc gccctgacgc tcgtcatcgg cgaggaggcg
     2881 cgggccgtct gcgaggggtt ctccccggag gatgtccggc gggcggtatc ggccgtcagc
     2941 ccgacggggg tcgtttacgt cggggaggcg tggagtgcgg ccggagagga gcacacggca
     3001 ccctcgctcg aagcggggct cgatgccgcc cggcggatca cccctgcggg cgcgatcgtc
     3061 cttgcggtaa agacgtggag atgaccatgg attacattca accaagaccg agttccattg
     3121 tagcggcgct ctataccgcc cgcgacctgg gggtggacgt ggcgatcctc cacggcccgt
     3181 caggctgttc gttcaaacac gcccgtcttc tcgaggagga cggcatgcgg gtgctgacga
     3241 cgtcgcttgc cgacaacgag ttcatcttcg gcggacacga ccccctcgtg cgggtcctcc
     3301 gccacgcgga gaaagagttt acgccccggc ggatggcggt cgtcgggacg tgtgtcgcga
     3361 tgatcatagg agaagatctc cagtccgcgc tccacgacgc cgggacccgg gtgcccgcta
     3421 tcgccgtgga tatccacgcc gggttccgcg aaaacatcga cggggtgctc gcgacgcttg
     3481 aaccggcggc ggctgccggc tggatcagcg gcgccgagct ggagcggcag cgctacctcc
     3541 tcggcaaggc aaacgaggtg gagaggctcc ggggggcggc gtcccggcac tacatcgagc
     3601 cctcccgcgg cgacctcaag cacgtcgcgg cacggcgcct cgtggacctc gccgactccg
     3661 gcgccatagg ggtcgcggtc atgaacgcga agaaagagac cgcgtatatg ttcgccgacg
     3721 agctcgtcgc cctccacgac acctgtccgg acgccgcgat cacctacatt gcgaacctcg
     3781 aagaccgggg cctcccgaag gtccgcaatg acgccgcacg gatcctcgcc ggcatgcggg
     3841 agcggggagt cgaccccgaa ctcctcgggg cgctcgacga gtacggcgcg aacggggatg
     3901 cgatcgggga gcggcttcgc gagatgaaac ccgacttcgc cctcatggtc ggcgtgccgc
     3961 acgccgtccc gccggagtac acggaaggga tcgaggtctt ctcgatcacg aacgggccgc
     4021 ggcaggtgga gccgctccgc gacatggggc accggcacgt catggtcgag atcgacctgc
     4081 acccaaagac cctcggcgtc cgcgagatcg tcgcaagcga gttcggggcg gtcctccgga
     4141 gcatgcgatg aagtccttcc tcgtcgccgg cgaccgctcc gggagcggga agacgagcat
     4201 cacccttgcg ctctctgccc tcctctcgac cacgcgggcc gtccagacct tcaaggtggg
     4261 gatggactac atcgacccct cctacctcac cggcgtgacg ggccggccct gccggaacct
     4321 ggacggctac gtgatgagcc cggccgaggt ccgggcggtc tttgcgcacg ggtgccgggg
     4381 agcggacgtc gccgtcgtcg aaggggtcag ggggctcttc gagggcgcgg aggcgctcac
     4441 cgacctcggg agcacggccg cgatagcgaa acaactcgac ctgccggtcg tcctggtcgt
     4501 caacgcccgg agcatcaccc ggagcgcggc ggctatcgtg aaagggttta tggccttcga
     4561 cccggacgtc gccatccggg gggtcatcct gaaccaggtg accggcgccc gccacaggga
     4621 gaaggcggtc agggcgatcg agcactactg cggcatcccc gtcgtcgggg tcatcccccg
     4681 gacggcggat atggaacttg ccatgcgcca tctcggcctc gtcccctacc gggaaggcca
     4741 ggagcacggg gagttcctcg cacgtatcga ggcggtgaag cagatgatcg gcgagcacgt
     4801 cgatatcgat gcgctgctct cgctcgcacg ggaggggaga gcgccgaccc cggaggaccc
     4861 catctacgcg gcagtcgatc cggacgtccg gatcggcgtc gccctcgatg aggcgttcaa
     4921 cttctactac gccgatctct tcgacgtgct cgcgtcctct ggggcagagg tggtcccgtt
     4981 ctccccggtc catggccggc tgccggaggc cgacggctac atcatcggcg gcggatcccc
     5041 ggaggtcttt gggccggagc tggaggcgaa caccgcggtg cgggaggggc tcagggaggc
     5101 ttcacgaaac ggcatcccgg tctacgcgga gtgcgggggg ctcgtctacc tcacggagag
     5161 gatggtgctc gcccccggct ttacagcctc gaagagggag gagacctacg accttgccgg
     5221 ggtctttgcc ggcgaggccc ggatgccgga gaagcggatg ctcggctacg tcgtggggac
     5281 gagcgcgggc gagaacccca tgggggcggc ggcgttcaag ggtcacgagt tccactacag
     5341 cagcgtccgc ctcgcgccgg agacgcgcta cgcctaccgg ctgacccggg ggagcgggat
     5401 ccgcgacggg cttgacggtg cggtccggga ccggacgatc gggagctaca cgcacctcca
     5461 cccggtgacg agccggggga tgttcgcaaa ctttgtggcg ggctgccggg gctgagagga
     5521 ggcgccggca ttcccggggg gagaggtcgt gccgccggga tcatgaagcg caagacatac
     5581 aaacgccccg gtctcatact gttcttatga ttatctacct tgacggcaga tacgtcccgg
     5641 aggaggaggc gaaggtctcg gtcttcgacc acg
//
LOCUS       DAOSII010000112         5668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_12829_length_5668_cov_45.229467, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000112 DAOSII010000000
VERSION     DAOSII010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12829_length_5668_cov_45.229467"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="PLV90_08225"
     CDS             complement(<1..1262)
                     /locus_tag="PLV90_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate pyruvate-lyase family protein"
                     /protein_id="HOF97140.1"
                     /translation="MEPLTADRISAKIHEIEQRVGKLSPMQKVLIGTDGSVTNLLEMA
                     TGHSVSITTRVQDIVAADTEAAAALAIEPGDEVNHRIVELKDSVTGNVLIYAVSRTPL
                     RRLAPEFRQDLMRADIPIGRILSRHRIESRREITDARVVQAGTDLARTFNIHRCESML
                     SRKYRIIHRGEPLIAIEEIFPCTAFADDVRVLVDAPSRLHITLLDMNGTSGRVDGGVG
                     VALDEPGCLLDARKGPGLDVHGGDELLRNRVLEAARAVKEGLGLPGGADITVHAVARP
                     HAGLGSGTQVALATAAALCRLYDRNVPVPDLARIVGRGGTSGIGTAAFAQGGFILDGG
                     HRFGSPGGKQDFRPSAASRGVAPPPVLARHRFPEDWRILLAIPDIDAGAHGRREIDIF
                     RTHCPVPLHEVRELCHEVLMRMIPGLVE"
     gene            complement(1327..2481)
                     /locus_tag="PLV90_08230"
     CDS             complement(1327..2481)
                     /locus_tag="PLV90_08230"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOF97141.1"
                     /translation="MKYILILGDGMADEPLAELGGRTPLEYADIPNMDRIAREGRCGM
                     LRTVPDGFEPGSDTANLSVLGYDPRTSYTGRGPLEAASMGVDLRKGEMAYRCNLVTIR
                     DGVMEDFNAGHISSTEGAGLLRDLDAAFEDVRVYPGVGYRNLMVVPGARGAVTTPPHD
                     IVGQAVRDYLPRGGDAAILLDCMERSREVFAGHPVNRRRLHEGKAPATGIWPWSGGEK
                     PSLAPFREKYGLSGGMISAVDLLNGIARLAGMEVIHVPGATGFLDTDYEAKARYAVDA
                     LDRLDFVYMHVEAPDEAGHMGSVEEKVRAIERLDEAIGIILDRPETAVAVLPDHPTPI
                     RLKTHTADPVPFAMLGEGRDGVRAFSERDAAKGSFGLVQAPDFLSLFFSC"
     gene            complement(2593..3540)
                     /locus_tag="PLV90_08235"
     CDS             complement(2593..3540)
                     /locus_tag="PLV90_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOF97142.1"
                     /translation="MFCIVTGGAGFIGSHIVDALVAEGNDVLVIDDCSAGTEENLARH
                     AAGGRVAFIRQNLLDDGWQEHFAGADRVYHIAADPDVRQSAVTPDSQVRNNVIATHRV
                     LEAMRAHDVPELVFTSTSTVYGEATVIPTPETYTPLEPISVYGATKLACEALISSYCH
                     SFGMRAWVYRFANIIGERSGHGVISDFIRKLRENPGELEILGDGRQTKSYLEVGECVR
                     AVQFGIDHSHDPANTFNIGSEDWIDVVTIADIVAEEMGLSGVRYRFTGGARGWVGDVP
                     KMQLSVEKLKALGWRCGTTSEESVRTAVRAMLGKEGARP"
     gene            3934..4809
                     /locus_tag="PLV90_08240"
     CDS             3934..4809
                     /locus_tag="PLV90_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HOF97143.1"
                     /translation="MSNPRPDIRGHAGIYLRGLMMGACDIIPGVSGGTIALITGIYER
                     LVGAIGSIDPASVKHLARGDFASFRRDLESLDIPFLVVLLAGIGTSFLLMSGIILSLL
                     ANHAVGTYSFFLGLILASAVLIFLEIRSPGAATLAYLAIGAGAGYLLGGLGNFDVGHS
                     LPVLFLTGMVALCAMILPGISGAYMTLVLNQYEFMLAALRALSLPEIVAYIAGGVTGL
                     LLFTRGLKYLLKTHPEAMLALLTGLMLGSARMLWERGSLAGDMVAGGSVFFIAGLVVV
                     GAVEYLKRRYAGSGA"
     gene            4875..>5668
                     /locus_tag="PLV90_08245"
     CDS             4875..>5668
                     /locus_tag="PLV90_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 12 protein"
                     /protein_id="HOF97144.1"
                     /translation="MFIGIDHGTTAMRFSTGMQEFKISREEARDFSASDLSRLSPLEE
                     IEGIAVCYSMGDGISAITDIRKVPDRGIVSREGAGKHIGGGTRVYDAIKESGLPAVVI
                     PGLHRGSPTDPRFKAYSHQASPEKIGILYEVVHDLGGDVVVCDASSNTVTLLVTGGRI
                     TGAFDACIFAPGTRHGALDVDAIRRIDRGESTANDAFLHAGVDNTMPPDLRVETMAMF
                     AAMECAAMLLINPGARVALAGSMAPAIAPGVKTLLARDVAVYDEWCA"
BASE COUNT          955 a         1886 c         1792 g         1035 t
ORIGIN      
        1 tgctcgacca ggcccgggat catccgcatg agcacctcgt ggcagagttc ccgcacctcg
       61 tgaagcggca ccgggcagtg cgtgcggaag atatcgatct cccgcctccc gtgcgccccg
      121 gcatcgatat cggggatggc aagcaggatg cgccagtcct ccgggaaccg gtgccgggcg
      181 agaaccggcg gcggtgcgac gccccgggat gccgccgaag gacggaagtc ctgtttgccc
      241 ccgggagagc cgaaccggtg cccgccgtcc agtatgaacc cgccctgcgc gaacgccgcc
      301 gtgccgatac cggatgttcc gccgcggccg acgatccgcg cgagatccgg caccggaaca
      361 ttccggtcgt agagcctgca gagggcggcg gccgtcgcga gagcaacctg cgtcccgctc
      421 ccgagccccg cgtgcgggcg ggccacggca tgcaccgtga tgtccgcgcc gccggggaga
      481 ccgagcccct ccttcaccgc ccgggcagcc tcaagaaccc ggttgcggag aagttcatcg
      541 ccgccgtgga catcgagccc cggccctttg cgcgcatcaa ggagacaccc gggctcgtcg
      601 agagcaaccc cgacaccgcc gtccaccctc ccggaggtgc cgttcatgtc gagcagcgtg
      661 atgtggagcc gggacggagc gtcgaccaga accctgacat cgtcggcaaa cgccgtgcag
      721 gggaagatct cctcgatcgc gatcagcggt tctccgcggt ggatgatccg gtacttccgc
      781 gagagcatcg attcgcaccg gtggatgttg aacgtccggg cgaggtccgt cccggcctgg
      841 acgacgcgcg catcggtgat ctcccggcgg gactctatcc ggtggcgaga aaggatcctc
      901 cctatgggga tatccgcccg catgagatcc tgcctgaact ccggggcaag ccgccgcaac
      961 ggtgtgcgcg agaccgcgta gatgaggacg tttcccgtaa cgctgtcttt gagctccacg
     1021 atgcggtggt tgacctcatc gccgggttcg atcgcaagcg cggccgccgc ttcagtatct
     1081 gcggcgacga tatcctggac gcgggtggtg atgctgacgg agtgaccggt cgccatctca
     1141 aggaggtttg tcaccgaacc gtcggtcccg atgaggacct tctgcatcgg ggagagcttt
     1201 cctacgcgct gctcgatctc atggatcttt gcgctgatcc tgtctgcagt caggggttcc
     1261 atgccagaat acgggtactg tacgcgcaga ctacaaaaag gtggtgccgg agtccggcct
     1321 gccggatcaa cacgagaaga agagggagag gaagtccgga gcctgcacga gtccaaacga
     1381 cccctttgca gcatcgcgtt cggagaacgc ccggacaccg tctctcccct ccccgagcat
     1441 cgcaaacggc accgggtcgg cggtatgggt cttcagccgt atcggcgtcg ggtggtcggg
     1501 gaggaccgca acggccgtct ccggccgatc caggatgatc ccgatcgcct cgtcgagccg
     1561 ttcgattgcc cgtaccttct cctccacgct ccccatgtgt ccggcctcat cgggagcctc
     1621 gacgtgcatg tagacaaagt ccaggcgatc gagggcatcg accgcgtacc gggctttcgc
     1681 ctcgtagtcg gtatcgagga accccgttgc gcccgggaca tggattacct ccatcccggc
     1741 aaggcgggcg ataccgttga ggaggtcgac ggcggagatc atcccgccgg aaagaccgta
     1801 tttctcacgg aaaggcgcca gcgacggttt ttccccgccg ctccacggcc agatcccggt
     1861 cgccggggcc tttccttcat gcaggcggcg ccggttgacc gggtggccgg caaagacctc
     1921 ccgggaccgc tccatgcagt cgaggaggat ggcggcatcg cctccgcgcg ggaggtagtc
     1981 tctgacggcc tggccgacga tgtcgtgggg cggggtggtg acggcgccgc gcgccccggg
     2041 aaccaccata aggttccggt aaccgacccc ggggtagacc ctgacgtctt cgaatgcggc
     2101 gtcgaggtcg cggaggaggc cggcgccttc ggtgctggat atgtgcccgg cgttgaagtc
     2161 ctccatcacc ccatcgcgga tggtgaccag gttgcaccgg taagccatct cccctttccg
     2221 gaggtcgacc cccatactgg ccgcctcgag cggccctctg ccggtatacg aggtacgggg
     2281 gtcgtagccc agtacggaga ggtttgcggt atcgctcccg ggctcaaacc catccgggac
     2341 agtccggagc atcccgcacc gtccctcacg ggcgatgcga tccatattcg gtatgtctgc
     2401 gtactcgagg ggtgtcctgc cccccagttc ggcaagcggt tcatccgcca tgccgtcccc
     2461 gagaatgagg atgtacttca tgtgaatcat gcgaatctgc tgtgtaatct cgatagtgct
     2521 ggttactcca atccggtcgc cggggccggc gacgaacgcc ctgccgccgg tcttgctaca
     2581 ggaagcgccc ggctagggcc gcgcaccttc cttcccgagc atggcgcgga ccgccgtacg
     2641 cacgctctct tctgacgtgg tcccgcaccg ccagccgagg gctttgagtt tctcgaccga
     2701 gagctgcatc ttcggcacat cgccgaccca gccgcgtgcg ccgccggtga accggtaccg
     2761 gacgccggag agccccatct cttccgcgac gatatcggcg atcgtgacga cgtcgatcca
     2821 gtcctcagag ccgatgttga aggtattcgc cggatcgtgc gaatgatcga tcccgaactg
     2881 cacggcgcgg acgcactcgc cgacctcaag gtatgacttc gtctgtcttc cgtcgccgag
     2941 gatctcgagt tctcccgggt tctcgcgaag ctttcggatg aagtcggata tgacgccgtg
     3001 gccgctccgc tcgccgatga tgtttgcgaa ccggtagacc cacgcccgca tgccaaacga
     3061 gtggcagtac gacgatatga gcgcctcgca ggcaagtttc gtcgcgccgt agacagatat
     3121 gggttcgagc ggcgtatagg tctccggcgt cgggatgacc gtggcctcgc cgtagacggt
     3181 cgacgtcgag gtgaagacca gttccggcac gtcgtgcgcc cgcatcgcct cgagcacccg
     3241 gtgcgtggca atgacgttgt tcctcacctg tgagtccggg gttacggcgc tctgccggac
     3301 atcagggtct gcggcgatgt ggtagacccg gtcggcgccc gcaaagtgct cctgccagcc
     3361 gtcgtcgagc aggttctgcc tgatgaaggc gacccggccg cccgccgcat gccgcgcgag
     3421 gttctcttcc gtgcccgcgc tgcagtcatc gatgaccagg acgtcgttcc cctccgcgac
     3481 cagagcgtca acgatgtgcg aaccgataaa accggcgcct cctgttacga tacagaacat
     3541 tgtagagtaa tagccttccc acagtatagg atttacttat ggggagtgcc ccggggcagc
     3601 ctcgttcccg ggtaaccgga ggtctcgccc gccgggcacc ccgaatacga cgcgacgata
     3661 gtggaaaaat ttatatctgc gggggatatc agggaataga gcggcactcc tccgccggga
     3721 gggggatggt ggccgcagag cctcgcctgt aagggcacgg caggttgcac caggacatat
     3781 tttttcaggg gtctcgccaa tatatggaga accataagga gttgtccaat tgagagaggt
     3841 atgcatccgt agcagtaccg gtccgttccc ggccgccggg tccggtggca tcccgtcgcc
     3901 gggatctgta gacaccgatc acggagccag gttatgagca acccgcgccc ggatatccgg
     3961 ggacacgccg ggatttacct gcgcggcctg atgatggggg cctgcgacat catccccgga
     4021 gtctcggggg ggacgattgc cctcatcacc ggcatctacg agcggctggt cggggctatc
     4081 gggagtatcg acccggcctc ggtaaagcac ctcgcgagag gcgacttcgc atccttccga
     4141 agggatcttg agagcctcga tataccgttc ctcgtcgtcc tccttgccgg gatcggcact
     4201 tctttcctcc tgatgtcggg gatcatcctc tctctcctcg cgaatcatgc ggtagggacg
     4261 tactccttct tccttggcct catcctcgcc tcggcggtcc tcatattcct tgagatccgt
     4321 tcccctggtg cggcgaccct cgcatatctc gccatcgggg cgggtgccgg ctacctcctt
     4381 gggggcctcg gcaacttcga cgtcggccac tccctgccgg tcctcttcct caccgggatg
     4441 gtggcgctct gcgccatgat cctccccggc atatcgggag cctacatgac cctcgtcctc
     4501 aaccagtacg agttcatgct tgcggcgctc cgggccctct ctctccccga gatagtcgcc
     4561 tacatcgccg gaggagtcac cgggctcctt ctcttcacca ggggcctcaa atacctcctt
     4621 aagacccatc cagaggcgat gctcgccctg ctcaccgggc tgatgctcgg ttcggcgcgg
     4681 atgctctggg agaggggatc ccttgccggg gatatggtgg ccgggggctc ggtcttcttc
     4741 attgccggcc tcgtcgtcgt cggggcagtc gagtacctga agaggcgcta cgcgggcagc
     4801 ggggcctgat ccgcgcccca tctcccgccg cgatcggaaa ggatactacc tccggaagga
     4861 agaagtacag tatcatgttc atcggcatcg atcacggcac taccgcgatg cgcttctcca
     4921 ccggaatgca ggagttcaag atctcccgcg aagaagccag agacttttcg gcctccgacc
     4981 tctcccgtct ctctcccctc gaggagatcg agggcatcgc cgtctgctac tccatgggcg
     5041 acggcatatc cgccatcacc gatatcagga aggtaccgga tcggggcatc gtctcccgcg
     5101 agggtgccgg caagcacatc ggcggcggca cccgggtcta cgatgcgatc aaagagagcg
     5161 ggcttcccgc cgtcgtgatc cccgggctgc accgcggctc cccgaccgac ccccggttca
     5221 aggcctactc ccaccaggca agcccggaga agatcgggat cctgtacgag gtcgtgcatg
     5281 acctcggggg cgacgtcgtc gtctgtgacg cgagctcaaa caccgtcacg ctcctcgtca
     5341 ccggcggccg gatcaccggc gcgttcgatg cctgcatctt tgcgcccggc acccggcacg
     5401 gcgccctcga cgtggatgcc atccgccgga tcgaccgggg cgagtccacg gcaaacgatg
     5461 ccttcctcca cgcgggcgtg gataacacga tgccccccga tctgcgggtg gagacgatgg
     5521 cgatgtttgc cgcgatggag tgcgccgcaa tgctcctcat caaccccggt gccagggtcg
     5581 cccttgccgg ctccatggcg cccgccatcg ccccgggcgt gaagacactc cttgcccggg
     5641 atgtcgcggt ctacgacgag tggtgtgc
//
LOCUS       DAOSII010000113         5644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_12886_length_5644_cov_56.906423, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000113 DAOSII010000000
VERSION     DAOSII010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12886_length_5644_cov_56.906423"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="PLV90_08250"
     CDS             complement(<1..1229)
                     /locus_tag="PLV90_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOF97145.1"
                     /translation="MGLTERQARAALDHTRSKCVTAGAGTGKTHVLVRKYISLLEEGA
                     SVGSILALTFTEKAAAEMKVRVRQALAEKEGKAWDAVRDEFLWAKISTFHAFCAAVLR
                     EFPLEANVGPSFTVLDEREAALLRDEAMDDLIYGDPPAEDREAVIGALRAVGAYELKN
                     YLIALSRHRDDAEAFFAALAENEEAVLDAWRAIVEEEREKASLAFRDRAGPSIEVLEG
                     LAARYPGKRDPAEAYLRAVERHLSGTVDALIEIHRGSGFRANMGQRKNWAGDDLNRLR
                     EAYVALKGCIEDNEAALSLALDPDDPFTRATLAFLHDLGKVFHAFIRAVEAEKRRRNA
                     LDFDDLVNRTRRLLVDHEEIAAHFRNRFRFILIDEFQDTDPVQIAIIHAILGREPGTL
                     FIVGDPKQSIYLFREADV"
     gene            complement(1216..4254)
                     /locus_tag="PLV90_08255"
     CDS             complement(1216..4254)
                     /locus_tag="PLV90_08255"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOF97146.1"
                     /translation="MSPAVLYRRLPGEGLDAFIAGFRKAAGHDPFGTVFIAPTASLAR
                     EVVRRLGEEGVPLVADAVTTLAGIARKIVSEHAARETLIPAAGSRLIIARLIAGGDYP
                     LLSGAGAVDDLAILFSVLTMRKVDYPAALGDLQGAKSAEIAALYRTYTRFLDEHRLID
                     ESTLTARAIRLLSGDFRTVYVYGLFEPMPLERDLLLALRESAEEFFYAIPCAANPAVF
                     ADDGGWLHPDEIIEGKQTSAVAGLFSRGPPGDYRGLIRIAERRDRLDEVRAIAQEIRD
                     LAAAGVRPGDIAVAFPDLDGAAPYVDEVFPDFGVPCTVSVGRPLSASPLVQALLSVLA
                     APARGYRRSDVIALVTSPYLPFTRGSELDILSREARIVAGTWDERLVALAANLDEQPE
                     PEAARDRNAEKIAAIEAARITIRRLFADLATLEGAKTIPDHIAAYRLLLDTWRAPVMP
                     GGGEPDLLDDEARDLAGFTRALAELEELARLLPGDKVPLSEFLSLLGILIAGSRAGRR
                     RNRNTVRVIGIREVPHISVPYLFIAGLVEGEMPRLTTRLPFLTDAETRRLGTRSKDDV
                     LREERYHFIAALLAAQSRVYISYPAADGSTPLVRSGFVDAVREAFAPETWGNSDFPDS
                     RLARAGRAGALLVRGEAANPPGLTVREAVCRLNIENYHRKGGYDSPYDGLIGDDPAIA
                     GRFGEEAVFSATALDTYADCPFRFSMEKVLGLAPLPPADPDLTAQERGNLVHRVACRF
                     YAGWRRDGNGPVTEAGLPDALRRIQATGREEADRFTFTSPAWVAEKEHLLGSPAAGPG
                     LLERFLRHDADMAASGLIPQAFEVSFGLPVSPGEVDLLSVPDAVAVPLGGETLRLRGR
                     VDRVDVLPDGRFAITDYKTGSRHPVLKDIRAGKALQLPLYMRAVEILTGMQGVAGTYY
                     TIRRGDVRNRPVFWDKAMKDCFGCFPGSQQSGVEDIRALVEGSLARAGEYLAGIRGGR
                     FPPRSDAGPCPVYCGFKTICRFDGLRLITAGGEVNADGTD"
     gene            complement(4398..>5644)
                     /locus_tag="PLV90_08260"
     CDS             complement(4398..>5644)
                     /locus_tag="PLV90_08260"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOF97147.1"
                     /translation="QDGNVVAAIETIRDITDRKRAEQEIVRSRRSLADIIDFLPDATF
                     AIDREGTVITWNREMEKLTGIPAASMIGRGDYEYSLPFYRDRRPMLANLVFIPDAELE
                     SLYTHVQREGNVLVVDTFIPGQEGRPGRFFWAKASPLYDPEGTVTGAIESIRDITERR
                     EMEGRLARSNAELQIAADIQMSFMPKVLPRIAGFDIAARAIMAKEVGGDFFDVIPFEI
                     VALDRGTLGLLIADVSGKGVPAALFMALSRIVVRVNALWHPDPAQAIYDANNIIAGDA
                     KSGMFVTLFFGRLREKDRTLTYVNAGHNPPVVFRSKTGVLEELPATGIILGAFENQDY
                     FSRSLVIEPDDVVVMYTDGVTEAIDARDEPFGVPRLNAIIRENARLPAQGILDRILSD
                     LHEFTGDTPQFDDITLLVIKGT"
BASE COUNT          934 a         1793 c         1973 g          944 t
ORIGIN      
        1 acgtcggcct cccggaagag gtagatggac tgtttcgggt cgccgacgat gaagagggtt
       61 cccggttccc ggccgaggat ggcgtggatg atggcgatct ggacggggtc ggtgtcctgg
      121 aactcgtcga tcaggatgaa ccggaaccgg ttccggaaat gcgccgcgat ctcttcgtgg
      181 tcgacgagga gccggcgggt gcggttgacc aggtcatcga agtcgagcgc gttacgccgc
      241 cgcttctcgg cctccaccgc ccgaatgaag gcatgaaaga ccttacccag gtcgtggagg
      301 aacgcaagcg tcgcccgggt gaaggggtcg tccgggtcga gggcgagcga gagggctgcc
      361 tcgttatcct cgatgcagcc tttgagggcg acgtaagcct cccggaggcg attgaggtcg
      421 tctcccgccc agttcttcct ctgccccatg tttgcccgga accccgagcc cctgtggatc
      481 tcgatgagcg catccaccgt cccggagagg tgcctctcga cggcccggag gtaggcctcg
      541 gccgggtccc gcttgccggg gtagcgggcg gcaagaccct cgagcacctc gatcgagggg
      601 ccggcacggt ctcggaacgc gagagaagcc ttctcccgct cttcctccac gatcgcccgc
      661 caggcgtcga gcaccgcctc ctcgttctct gcaagggcgg caaagaacgc ttcggcgtca
      721 tcgcggtgcc tggagagtgc gataaggtag ttcttcaact cgtaggcccc taccgcccgg
      781 agggcaccga tcaccgcctc ccggtcctcc gccggcgggt cgccgtagat gaggtcgtcc
      841 atcgcttcat cgcggagcag ggccgcctcc cgctcatcga ggacggtaaa agaggggccg
      901 acgttcgcct cgagcggaaa ctcgcggagg accgcggcgc agaaggcgtg gaaggtcgat
      961 atcttcgccc agaggaactc atcgcggacc gcatcccacg ccttgccctc cttctcggcg
     1021 agggcctgcc ggaccctgac cttcatctcc gcggccgcct tctcggtgaa ggtcagggcg
     1081 aggatgctcc cgacactggc gccctcctca aggaggctga tgtatttccg gaccaggaca
     1141 tgcgtctttc ctgtcccggc cccggcggtg acgcacttgc tcctcgtgtg gtcgagcgcg
     1201 gcccgcgcct ggcgttcagt cagtcccatc ggcgttcacc tccccgcccg cggttatcaa
     1261 ccgcaagccg tcgaaccggc agatggtctt aaatccgcag tagaccgggc aggggccggc
     1321 atccgaccgg ggcgggaacc gcccgccgcg tataccggcg aggtactctc cggcccgggc
     1381 gagggagccc tcgaccagcg cccgtatatc ctcgacaccg ctctgctgcg atcccgggaa
     1441 gcagccaaaa cagtctttca tcgccttatc ccagaagacg ggccggttcc ggacatcccc
     1501 gcgcctgatg gtatagtagg tgccggcgac tccctgcatc ccggtcagga tctcgaccgc
     1561 ccgcatgtag agcgggagct ggagcgcctt ccccgcccga atatccttga ggacagggtg
     1621 cctggatccg gtcttgtagt cggtgatcgc gaaccggccg tcgggcagga cgtcgacccg
     1681 gtcgaccctc ccccgcaggc ggagggtctc gccgccgagc gggaccgcga ccgcatcggg
     1741 gaccgatagg aggtcgacct ctcccggcga gaccgggagg ccgaacgaga cctcgaaggc
     1801 ctgcgggatg agaccggaag ccgccatatc cgcatcgtgc cggaggaacc gctcaagcag
     1861 ccccgggccg gccgcgggcg atccgaggag gtgctccttc tccgcgaccc aggccgggct
     1921 cgtgaacgtg aaccggtcgg cctcttcccg gccggtggcc tggatccgcc ggagggcgtc
     1981 cgggaggccg gcttcggtga ccgggccgtt tccgtcccgc cgccagccgg cgtagaaccg
     2041 gcaggcgacc cggtggacca ggttcccccg ctcctgtgcg gtcaggtcgg gatcggccgg
     2101 gggcagcggc gcaagaccga ggaccttctc catggagaac cggaacgggc agtcggcgta
     2161 ggtatcgagc gccgttgccg agaagacggc ctcttcaccg aatcggccgg cgatcgccgg
     2221 gtcatccccg atcaggccgt cgtaggggga gtcgtagccg cctttccggt ggtagttctc
     2281 gatattgagc cggcaaacgg cctcgcgtac cgtaagcccc gggggatttg ccgcctctcc
     2341 ccggacgaga agggcgccgg cccgcccggc tctcgcgagc cgcgagtccg gaaagtcgct
     2401 attcccccac gtctccgggg caaaggcttc ccggacggcg tccacgaacc cggagcggac
     2461 aaggggcgtg ctgccgtcgg ccgcgggata actgatgtag acccgggatt gggcggcaag
     2521 aagcgccgcg atgaagtggt agcgttcctc gcggaggacg tcgtcttttg aacgggtgcc
     2581 gaggcgccgg gtctcggcat cggttaagaa cgggaggcgg gtggtcagcc gcggcatctc
     2641 tccctcgacg aggccggcga tgaagaggta ggggacggag atatgcggga cctcgcggat
     2701 accgatgacc cggacggtgt tcctgttccg tctccggccg gcccgggatc cggcaatgag
     2761 gatgccaagg agggataaga actcggaaag cggcaccttg tcgcccggga ggagccgggc
     2821 gagttcttcg agttccgcga gggctctcgt gaacccggca aggtcgcggg cctcgtcgtc
     2881 taataggtcg ggttctcccc cgccgggcat caccggggcc cgccacgtat cgaggagcaa
     2941 gcggtaggcg gcgatgtgat ccgggatcgt ctttgctccc tcaagggttg caaggtcggc
     3001 aaagagccgg cggatagtga tccgtgccgc ctcgatcgcg gcgatctttt ctgcgttccg
     3061 atctcttgcg gcctccggtt ccggctgctc atcgaggttc gcggcgagtg ctacaagccg
     3121 ctcatcccag gtgccggcaa cgatccgggc ctcccgcgag aggatgtcga gttcggaccc
     3181 ccgggtaaac ggcaggtagg gggaggtcac gagggcgatg acgtcagaac gcctgtagcc
     3241 gcgggccggg gcggcgagga ccgagaggag cgcctggacg agcggcgatg cggagagcgg
     3301 ccggcccacg gagacggtgc agggaacccc aaagtcgggg aagacctcgt cgacgtaggg
     3361 cgccgccccg tcgagatcgg ggaacgcgac ggcgatgtcg cccggccgga cgccggcggc
     3421 ggcaagatcg cggatctcct gcgctatcgc ccggacttcg tcgagccggt ctcgccgttc
     3481 tgcgatgcgg atgagccctc gatagtcccc gggcgggccg cgggagaaga ggccggcgac
     3541 cgccgaggtc tgtttgccct cgatgatctc gtcggggtgc agccacccgc cgtcgtccgc
     3601 gaagaccgcc gggtttgcgg cgcagggtat cgcgtagaaa aactcctccg cggactcccg
     3661 gagagcgagg aggaggtcgc gctcgagcgg catcggctcg aagagcccgt agacgtagac
     3721 cgtacggaag tcgcccgaaa ggagccggat tgcccgggcg gtaagggtgc tctcgtcgat
     3781 gaggcggtgc tcgtcgagga accgggtgta ggtgcggtag agggcggcga tctcggcgct
     3841 ctttgcgccc tgcagatccc cgagggcggc cgggtagtcg accttccgca tggtgaggac
     3901 cgagaagagg atagcgagat cgtcgacggc gccggcaccg gagaggagcg ggtaatcgcc
     3961 gccggcgatc agccgggcga tgatgagccg tgacccggcc gcgggaatca aggtctctct
     4021 cgccgcgtgt tcggaaacga tctttctggc gatgccggcg agggtcgtga ccgcgtcggc
     4081 gacgagcggg accccttcct ccccgaggcg gcggacgacc tcccgggcga ggcttgcggt
     4141 cggggcgatg aagacggtcc cgaacgggtc atggccggcg gctttccgga aaccggcgat
     4201 gaacgcatcg agaccctctc cgggcaatcg gcggtagaga actgctgggg acaatcgata
     4261 atctctcctt acgagaggta gggggcggga gagggatgag ggttgttatt tgggtttggg
     4321 gaggccctct tttcagtctg cgaccggagg atcggccgct gcccccatcg cctcgagccc
     4381 ggggagaaat tccgccatca cgttcctttg atcaccagca gcgtgatgtc gtcaaactgg
     4441 ggcgtatccc cggtgaactc atggagatcg gataatatcc ggtcgaggat cccctgtgcc
     4501 ggaagccggg cattctcccg tatgatcgcg ttgagccggg gcaccccgaa cggttcatcc
     4561 cgggcgtcga tcgcctccgt gacgccatcg gtgtacatga caaccacatc gtccggctcg
     4621 atcacgagcg accgggagaa gtaatcctgg ttctcgaacg cgccgaggat gataccggtt
     4681 gccgggagtt cctcgagcac gccggttttg ctccggaaga ccaccggggg gttgtggccg
     4741 gcgttgacgt aggtcagggt ccggtctttc tcgcgcagtc tcccaaagaa gagggtgacg
     4801 aacatcccgg acttcgcgtc ccctgcgatg atgttgttgg catcgtagat cgcctgcgcc
     4861 gggtcagggt gccagagcgc gttcacccgg acaacgatac gggacagcgc catgaagagg
     4921 gctgccggga cgcctttccc cgagacgtcg gcgatgagga ggccgagggt tccccggtcg
     4981 agtgcgacga tctcaaaggg gatcacgtcg aagaagtcgc cgccgacctc tttggccatg
     5041 attgcgcggg cggcgatatc gaaccctgca atccggggga gcaccttcgg catgaaactc
     5101 atctggatat ccgcggcgat ctggagctcg gcgtttgacc gggcaagccg cccctccatc
     5161 tcgcggcgtt cggtgatgtc ccggatcgac tcgatggcgc cggtgacggt gccctccggg
     5221 tcatacagcg ggcttgcctt ggcccagaag aacctgcccg gccggccttc ctggcccggg
     5281 atgaacgtat ccacgacgag cacgttcccc tcccgctgca cgtgggtgta gaggctctcc
     5341 agttccgcat ccggtataaa gacgaggttg gccagcatcg gcctcctgtc ccggtagaac
     5401 gggagggaat actcgtaatc ccctctcccg atcatggatg cggcggggat gccggtcagt
     5461 ttctccatct cgcggttcca ggtgatgacg gtgccctccc ggtcgatggc aaacgtggca
     5521 tcggggagaa aatcgatgat atccgcaagg ctccgccggg accggacgat ctcctgttcg
     5581 gcgcgttttc ggtcggtgat gtcgcggatc gtctcgatgg cggcaacgac gttgccgtcc
     5641 tggt
//
LOCUS       DAOSII010000114         5589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_13028_length_5589_cov_47.122877, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000114 DAOSII010000000
VERSION     DAOSII010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13028_length_5589_cov_47.122877"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLV90_08265"
     CDS             complement(<1..391)
                     /locus_tag="PLV90_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF97148.1"
                     /translation="MAIRDPGRGFHCRVALRISSNGRNAYRLTRIHVNGEEFAVRTMR
                     QREVEIAVDWAAQEGWNPGVHDADAFYAADPEGFFIAELDGEPIGSLSVVNYSETFAF
                     AGLYILRPEYRNRGYGSRLFAAGMAHAG"
     gene            650..1951
                     /locus_tag="PLV90_08270"
     CDS             650..1951
                     /locus_tag="PLV90_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HOF97149.1"
                     /translation="MKEDLGQIIDRSFRPATEILLDLLDDGFKCAFSISGVVVEHLER
                     WYPEMLELISRAATHRNAEILAETYYHSVAGQFADLAEFTDELLMHRDLMKEQFATVP
                     TTFVHTDYPITPATADVLAGAGIEAAIIEGGAPPAPEGDPNHTYTYHGLPVLVTHCDL
                     TDDIAVRFPSREWDRWPLMADRYAEWLAVSPGDCITLFLDYRIFGDVIGPETGIFDFL
                     RALPAALAGEGIMTLHPSEAVRTIPPQADIEETGGPQRTILQQSALEALEEAGDLVTD
                     RAVWRCLLETDHIRRMAMRSPSCGRSLHAVSHQATYDYFTSFMQILSHAEERSASRTR
                     SPKAALSLRCIPPDKAFSFSSYDRPAGYAAHSLQELASMLDFTPDDVIRYHVERDDIY
                     RWIDQVVGDGKLAKKVQGISDRNELRSTIQKRIDELWKRLR"
     gene            1933..3084
                     /locus_tag="PLV90_08275"
     CDS             1933..3084
                     /locus_tag="PLV90_08275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOF97150.1"
                     /translation="METLKIAFFCWESLHSPFKVGGLAPAATHLAEHLARRGHEVHFF
                     TRGEPGDAEVNGIHYHYCRPYGDNIIEYCKTMSNMLVDAFRACDTPAFDILHFHDWHL
                     VEALHTLRDRNTVLSYHSTEYGRHGGNFGGWWEFKEISGKEWYGGYIAKTVTTVSNST
                     KTEVMWLYNVPEWKVTVIPNGIDPDAYYARVDPGEVKKRYGIHPLAPVVLFVGRLTYQ
                     KGPDLLMQAIPRILATRWDVQFLFAGTGDMRGYLEGMAHGLPVQFLGYIADREHVQLL
                     NACDLIAIPSRNEPFGLVLTEAWSAERCVVATDVGGLSENIDNYINGIKVPVRPDSIA
                     WGICHLIDDPVYMQSLGKAGRRKVMEKFRWAVITERMLSVYRKVLSDSP"
     gene            3100..5031
                     /gene="glgB"
                     /locus_tag="PLV90_08280"
     CDS             3100..5031
                     /gene="glgB"
                     /locus_tag="PLV90_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOF97151.1"
                     /translation="MQQALPDITRTRSLITDYDVYLFRQGSHSRLYDRLGSHPAVIDD
                     RRGTFFAVWAPNAVEVSVIGDFNGWKAGENPLSLRNDDSGIWEGFLPDIGQGTLYKYH
                     IRSRYNSYSVEKGDPFARFWEIPPKTASIIWDIAYTWRDRAWMRGREEENSPDAPISI
                     YEVHAGSWRKVPEEENRSLSYREAADELAEYLVDAGFTHVEFLPIMEHPFYASWGYQT
                     LGYFAPTSRYGTPQDFMHLIDHLHQRGVGVILDWVPSHFPADGYGLSYFDGTYLYEHA
                     LPDRRYHPEWKSYVFNLARNEVRSFLLSSAVFWLDRYHIDGLRVDAVSSMLYLDYARG
                     AGEWTPNTYGGRENLEAIGFLRKVNDTVHANFPDVLVIAEEATSWPGVTSPTATGGLG
                     FDLKWNMGWMHDTLDYFTLDPILRKYRLDLLTFSIWYAFSERYVLPLSHDEVVHEKSS
                     LIGKMPGDEWRKRANLRLLYGYMFTHPGKKLLFMGGEFGQWSEWSHDAGLERHLLQYS
                     PHQGILQWVTDLNRLYRREPALHERDADPEGFEWVDFSDVDKSIVSYLRRGRSADDVV
                     LVACNCTPVPRYNYRVGVPFGGFWEEVLNSDAVEYGGSGVGNLGGVEAEQVPAHGRPW
                     SLPLTLPPLAVVILAPEGA"
     gene            5028..>5589
                     /locus_tag="PLV90_08285"
     CDS             5028..>5589
                     /locus_tag="PLV90_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HOF97152.1"
                     /translation="MTMDRAVCIHSHFYQPSRENPWLGDVEVQRSATPYHDWNERVTA
                     ECYGPNTAARILDADGLIDEIVNTYAKVSFTAAPTLLSWLERHSPAVYRAIVDADRES
                     REHYAGHGSAIASCYSHLIMPLATRREKQVQIAWGIRDFTARFGREPEGMWLPETAVD
                     IESLDLMAGAGIRFTILAPHQAGRVRA"
BASE COUNT         1107 a         1841 c         1639 g         1002 t
ORIGIN      
        1 ccccggcgtg ggccatgccg gcggcaaaca gcctgctccc gtagcccctg ttccggtatt
       61 cgggcctcag tatatacagg ccggcaaagg caaacgtctc gctgtagttg acgaccgaga
      121 gcgatcctat cggctcgccg tcgagctcgg cgatgaagaa cccctccgga tcggctgcat
      181 aaaaagcatc cgcatcgtga acccccggat tccacccctc ctgtgccgcc caatcgacgg
      241 caatctcgac ctcccgctgc cgcatcgtcc tcacggcaaa ctcctctccg ttcacgtgaa
      301 tcctcgttaa gcggtaagcg ttcctcccat ttgaggatat ccggagagca acacggcagt
      361 gaaaccctct cccgggatcc cggatggcca tgatcccacc gggaggtagg atctaccctt
      421 tattcgggca tcaggaccct gtggagggca aaagaccaaa agatcttccg ggttgatgac
      481 ctttatatac ggaaacagcg acctacctcg tatgagaggc gagggcgcaa atccgccggc
      541 ggtctgtctg gggttcgaca tccactatcc ctgttacctc aatcccggat ttcgaccaga
      601 tatggtaaag ggaaagagaa acccaaagga tagctacttt aatccggata tgaaggaaga
      661 tctggggcag atcatcgacc ggtccttccg accggcgacg gagatcctcc tcgatctcct
      721 cgatgacggg tttaagtgcg ctttcagtat atccggggtg gtggtggaac acctcgaacg
      781 gtggtatccg gagatgctcg aactcatatc ccgcgcggca acccaccgga acgccgagat
      841 cctcgcagag acctactacc acagcgttgc cgggcagttc gccgatctgg cggagttcac
      901 ggacgagctt ctcatgcacc gcgacctgat gaaggagcag ttcgcaaccg tcccgaccac
      961 gtttgtccat accgattatc ccatcacccc ggccaccgca gatgtgctcg ccggtgccgg
     1021 tatcgaggcg gctatcatcg aggggggcgc gccccccgcc cccgagggag acccaaacca
     1081 cacctacacc taccacggcc tcccggtcct cgtcacccac tgcgacctca ccgacgacat
     1141 cgccgtgcgg tttccctcaa gggagtggga taggtggccg ctgatggccg accggtatgc
     1201 agagtggctt gccgtatccc ccggcgactg catcacgctc ttcctcgact accgcatatt
     1261 cggagacgtc atcggtcccg aaacggggat cttcgatttc ctgcgcgcgc tcccggcagc
     1321 cctggccggg gaggggataa tgaccctgca cccatcagaa gctgtgcgga ccatcccccc
     1381 gcaagcggat atcgaagaga ccgggggtcc gcaaaggacc atcctgcagc agtccgcact
     1441 cgaggcactt gaggaagccg gcgatctggt gactgaccgg gcagtctggc gctgcctcct
     1501 tgagaccgac catatccgga ggatggccat gcgctcgccc tcgtgcggaa gatccctcca
     1561 cgccgtcagc caccaggcaa cctacgacta cttcacctcg tttatgcaga tcctctccca
     1621 cgccgaagag cggtctgcgt cccgcacacg gtcaccgaag gccgccctct cgctccggtg
     1681 catccccccc gataaggcgt tctccttctc gtcgtacgac agaccggccg ggtatgccgc
     1741 ccacagcctg caggaacttg ccagcatgct cgattttact ccagacgacg tcatccggta
     1801 ccatgtagag cgggatgaca tttaccgctg gatcgatcag gtcgtcggcg atgggaaact
     1861 ggcgaagaag gtccagggca tcagcgatcg caatgaactc cgctcaacaa ttcaaaagag
     1921 gatcgacgag ttatggaaac gcttaagata gcattcttct gctgggagtc gctccactcc
     1981 ccgttcaagg tgggcgggct cgcaccggcg gcaacccacc ttgccgaaca tcttgcccga
     2041 agagggcacg aggtccactt cttcacccgg ggagaacccg gggatgcaga ggtcaacggc
     2101 atccactacc attattgccg cccttacggc gacaacatca tcgagtactg caagaccatg
     2161 agcaacatgc tcgtggatgc gttccgcgcc tgcgacaccc cggcgttcga tatcctccac
     2221 tttcacgact ggcatcttgt cgaggcgctg cataccctcc gggaccgaaa caccgtcctc
     2281 tcttaccact ccaccgagta cgggcggcac ggcgggaact tcggcggttg gtgggagttc
     2341 aaagagatat cgggcaagga gtggtacggc ggctacattg caaagacggt cacgaccgtc
     2401 tcgaactcga cgaagaccga ggtgatgtgg ctctacaacg tccccgagtg gaaagtcacc
     2461 gtcatcccga acggaatcga tcccgatgcc tattacgccc gggtcgaccc tggcgaggtg
     2521 aagaaacgct acggcatcca cccgctcgca cccgtcgtcc tcttcgtcgg gcgcctcacc
     2581 taccagaaag ggcccgacct cctgatgcag gccatacccc ggatcctcgc cacacgctgg
     2641 gacgtgcagt tcctcttcgc cggcaccgga gatatgcgcg gctacctcga ggggatggcc
     2701 cacggccttc ccgtgcagtt cctcggctac atcgccgata gagagcacgt ccagctcctg
     2761 aacgcctgcg acctcatcgc cataccgagc aggaacgaac cgttcgggct cgtcctcacc
     2821 gaagcctgga gcgcggagcg ctgcgtcgtc gcgaccgatg tcggggggct ctccgagaac
     2881 atcgacaact acataaacgg gatcaaggtc cctgtccgac cggactcgat cgcctggggg
     2941 atctgccacc tcatcgacga cccggtctac atgcagagcc tcggaaaagc cggccgccgg
     3001 aaagtgatgg agaagttccg gtgggccgtc atcacggaga ggatgctatc cgtctacaga
     3061 aaggttctct ccgacagtcc ctgatcccgg agggagacca tgcagcaggc gctacctgac
     3121 ataacgagga cccggagtct catcaccgac tacgacgtct acctcttccg gcagggaagc
     3181 cattcccgcc tgtacgatcg cctgggatcg caccctgccg tcatcgacga cagacgggga
     3241 accttctttg cggtctgggc gccgaacgcg gtggaggtct cggtcatcgg agacttcaac
     3301 gggtggaagg cgggcgagaa tccgctctct ctccggaacg acgattccgg tatctgggaa
     3361 gggtttctgc cggatatcgg ccagggaacg ctctacaagt accatatccg gagcaggtac
     3421 aacagctact ccgtggagaa aggcgatccg ttcgcccgct tctgggagat cccgccaaag
     3481 actgcatcca tcatctggga tatcgcctat acatggcgcg atcgggcctg gatgcggggc
     3541 cgcgaggagg agaactcccc cgatgccccg atatccatat acgaggtgca tgcaggctca
     3601 tggcggaagg tgccggagga ggagaaccgt tccctcagtt accgggaggc ggcagacgaa
     3661 ctcgccgaat acctggttga tgccgggttc acccacgtcg agttcctccc catcatggag
     3721 cacccgttct acgcctcctg gggctaccag acgctcggct acttcgctcc cacgagcagg
     3781 tacggcaccc cgcaggactt catgcacctc atcgatcacc tccaccagcg cggggtgggg
     3841 gtgatcctgg actgggtgcc gtcccatttt cctgccgacg gctacggtct ctcttacttc
     3901 gacggtacct acctctacga acacgccctc cccgatcgac gctatcaccc cgaatggaag
     3961 agttacgtct tcaacctcgc aaggaacgag gtccggtcat tcctcctgag cagcgccgtc
     4021 ttctggctcg atcgctatca tatcgacggc ctccgggtcg atgcggtctc ctcgatgctc
     4081 tacctcgact acgcgcgggg cgcgggggaa tggaccccga acacctatgg cgggagggag
     4141 aacctcgagg caatagggtt cctccgaaag gtgaacgata cggttcatgc aaacttcccg
     4201 gacgtgctcg tcatcgccga ggaggcgacc tcctggccgg gggtgacctc cccgaccgcg
     4261 accggggggc tcgggttcga cctgaagtgg aacatgggct ggatgcacga caccctggac
     4321 tacttcacgc tcgatccgat cctccggaag taccggctcg acctcctcac gttcagcata
     4381 tggtacgcgt tctcggagcg ctacgtcctc cccctctcgc acgacgaggt cgtccacgag
     4441 aagagctcgc tcataggaaa gatgcccggc gacgagtggc ggaaacgggc gaacctccgc
     4501 ctcctctacg gctacatgtt cacccacccg ggaaagaagc tcctcttcat ggggggtgag
     4561 ttcgggcagt ggtcggagtg gagtcacgat gccgggcttg aacggcacct cctgcagtac
     4621 tccccccacc aggggatcct ccagtgggtc accgacttaa accggctgta ccggcgggag
     4681 cccgccctcc acgagcggga cgccgacccg gaggggtttg agtgggtcga cttctcggat
     4741 gtggataaga gcatcgtcag ctacctgcgg cggggacgat cggcggacga cgtcgtcctg
     4801 gtcgcctgca actgcacacc ggtcccgcgc tacaactacc gtgtcggcgt cccgttcggc
     4861 ggtttctggg aggaggtgct caacagcgat gcggtcgagt acggtggaag cggtgtcggg
     4921 aacctcggtg gggtggaggc ggagcaggtc ccggcgcacg gccggccgtg gtcgctcccg
     4981 ctcaccctgc ccccgcttgc ggtcgtcatc ctcgcccccg agggggcatg acgatggatc
     5041 gcgccgtctg catccacagt cacttctacc agccttcacg ggagaacccc tggctcggcg
     5101 acgtcgaggt ccagcgctcc gcaaccccct accatgactg gaacgaacgg gtgaccgcgg
     5161 aatgttacgg cccgaacacc gccgcacgca tcctcgatgc ggacggcctg atcgacgaga
     5221 tcgtcaacac ctacgcaaag gtcagtttta cggccgcacc gacgcttctc tcctggcttg
     5281 aacggcacag tccggcggtc taccgggcga tcgtcgatgc ggaccgggag agccgggaac
     5341 actatgccgg ccacgggtcc gcgatcgcct cctgctacag ccacctcatc atgccgcttg
     5401 cgacacggcg ggagaaacag gttcagatcg cctggggtat ccgcgacttt accgcacggt
     5461 tcggccggga acccgaaggg atgtggctcc ctgagacggc ggtcgacatc gagtccctgg
     5521 acctgatggc cggtgccggg atccggttca cgatccttgc cccccaccag gcagggaggg
     5581 tgcgggcga
//
LOCUS       DAOSII010000115         5505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_13275_length_5505_cov_50.758349, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000115 DAOSII010000000
VERSION     DAOSII010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13275_length_5505_cov_50.758349"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..439)
                     /locus_tag="PLV90_08290"
     CDS             complement(17..439)
                     /locus_tag="PLV90_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97153.1"
                     /translation="MRRIYHRFPQSIDLDFELVQPFRGILTCLARMHDTHTTVKEADG
                     LVKERIHIQIADGRTKFLDLDDLMPLSEKVTETADFRVSLQRTLLTPEKRLPVAENVF
                     YLRIIDKGPVTECYIAKEGRLGGLDAMDLRRILKGACE"
     gene            530..>1927
                     /locus_tag="PLV90_08295"
     CDS             530..>1927
                     /locus_tag="PLV90_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HOF97154.1"
                     /translation="MADRLTLLQVNDVHGYLELHPELFWTGEQARYRMAGGYARIATL
                     FEDARDERPGQVLAFDCGDTIHGTYPAVQSEGEALIPVLNTLGFTAMTAHWEFAYGPE
                     QFRKVARALDYPVLADNCYDDATGDLIFPPYAVCETDDLQVGVIGIAATIVDKVMPPS
                     FSEGIHFTLGNAELPEHIARLRDDEGVDIVVVMSHLGFPKEVKLAREVEGIDILLSGH
                     THDRLFEPAIVNDTIIIQSGCHGSFLGRLDLTVEDRRVTKFDHELIVVDEGVRPEPGV
                     EEMVEGIMEPHRERLSRVVGETRTGLNRNTAIEATMDNFLLQALLDCTGADMAFSNGW
                     RYGAPVPPGPVTVNDLWNIIPTNPPVSTVDITGRELRTMMEEDLEHTFARDPYNQMGG
                     YVKRCMGVTLYCRLENAPGLRIEDFFVGGKRLDLDAAYRAAFVTTQGVPERYGRNRED
                     LDTLAVEALERYLARG"
     assembly_gap    1928..2027
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2039..2416)
                     /locus_tag="PLV90_08300"
     CDS             complement(2039..2416)
                     /locus_tag="PLV90_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8e"
                     /protein_id="HOF97155.1"
                     /translation="MQWQGRSVRKPSGGRYHTSQGKKRSEIGRAPAETHIGEERKKVV
                     RTYGGNQKVRALRLDYATVANPATGETHKAKIEAVEANSANPNYVRRSLLTKGAVIRT
                     DVGRARIVSRPGQDGVVNAVLIA"
     gene            complement(2522..3169)
                     /gene="hypB"
                     /locus_tag="PLV90_08305"
     CDS             complement(2522..3169)
                     /gene="hypB"
                     /locus_tag="PLV90_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOF97156.1"
                     /translation="MHHIDVHVEKDIYDVNNRIADANAEHLREHGIRAFDLLGAIGSG
                     KTATIERLVPLLRERGIRAGAIAGDVYGDDDFKRIVSLGVPACNVNTGKECHLDAHLV
                     EHAIEHLPLDDIDILFIENVGNMICPTDFRLGAEKRIVVISSTEGDDVVNKHPMMFRG
                     SNIGVINKVDLAPFVGADLDRMERDMRRYNPEMQIFRTNMKTGEGIRELLDAILA"
     gene            3224..3493
                     /locus_tag="PLV90_08310"
     CDS             3224..3493
                     /locus_tag="PLV90_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle subunit SRP19/SEC65
                     family protein"
                     /protein_id="HOF97157.1"
                     /translation="MSAERILYPCYFDGTLQRREGRRVSRNLGVKSPDLPAIETALRK
                     MKVPYRVEAHHHPAWWAEREGRVAVEWKGSKEDLIRKVAREIGRK"
     gene            3499..4143
                     /locus_tag="PLV90_08315"
     CDS             3499..4143
                     /locus_tag="PLV90_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HOF97158.1"
                     /translation="MYDLHTHTLLSDGELLPTELVRRAAVLGYKTLAITDHADASNLV
                     HLVDAVSMVRDAARWYGVDLLVGVELTHVPPSLIPALAREAKERGADIVVVHGETVME
                     PVAPGTNRAACTCEYVDVLGHPGLITVEDARAAAEHGVALEITSRAGHNRTNGHVVGV
                     AREAGCLLTVDSDTHAPSDLMAEGARWEVALGAGLTEAESRDVLSRDVKRFLQK"
     gene            4342..4470
                     /locus_tag="PLV90_08320"
     CDS             4342..4470
                     /locus_tag="PLV90_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97159.1"
                     /translation="MDRRLKPIGKVVDIFGNISSPYAVVLCYNGCPVQVGEKIFAK"
     gene            4482..5495
                     /locus_tag="PLV90_08325"
     CDS             4482..5495
                     /locus_tag="PLV90_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription initiation factor IIB"
                     /protein_id="HOF97160.1"
                     /translation="MAEVEKLKQLQLQREALKKRGEQKVKETEKKRTEESVQSVCPEC
                     GSRQLVHDYERAELVCQSCGLVLDEEFIDRGPEWRAFDHDQRMKRSRVGAPMTFTIHD
                     KGLSTMIDWRNRDSYGRAISSKNRAQLYRLRKWQRRIRVSNATERNLAFALSELDRMA
                     SALGLPRNVRETAAVVYRDAVDKNLIRGRSIEGVAAAALYAACRQCSVPRTLDEIAEV
                     SRVSRKEIGRTYRFISRELGLKLLPTSPIDYVPRFCSGLNLKGEVQSRAVEILRQAGE
                     RELTSGRGPTGVAAAAIYISSILGGERRTQREVAEVAGVTEVTIRNRYKELAEKLDIE
                     IIL"
BASE COUNT         1007 a         1608 c         1697 g         1093 t
ORIGIN      
        1 accgcaatct gcacgctcac tcgcaggcac ccttgaggat tctccgcagg tccatcgcgt
       61 ccaggccgcc cagtcgtccc tcttttgcga tgtagcactc tgtcaccggc cctttatcga
      121 tgatcctgag ataaaagacg ttctctgcaa ccggcaacct cttctcgggg gtgaggagcg
      181 ttctctgcag gctgacgcga aagtctgccg tttcggtgac tttctcggag agcggcatca
      241 ggtcgtcgag gtccaggaac tttgtccgcc catccgcgat ctggatgtgg atccgttcct
      301 tgacgaggcc gtctgcctcc ttcactgtcg tgtgggtgtc gtgcatccgg gccaggcagg
      361 tgaggatgcc tcgaaatggt tggacaagtt caaaatcgag gtcgatggac tgggggaaac
      421 gatggtatat cctgcgcatc actagaattg atacccgcag aacgtaaaaa gcatccgatc
      481 ccgccggaag cgcaaagatt tttggcacac ccgccccttc cccgcaccca tggcggatcg
      541 ccttacgctc ctgcaggtga acgacgtgca cggctacctg gaactgcacc ccgagctctt
      601 ctggaccggc gaacaggccc ggtaccggat ggccgggggg tatgccagga ttgccactct
      661 cttcgaagat gcccgcgatg agcgaccggg tcaggtgctt gcgtttgact gcggcgacac
      721 catccacggg acctacccgg ccgtccaatc ggagggtgag gcgctcatcc cggtcctcaa
      781 caccctcggg ttcaccgcta tgaccgcgca ctgggagttt gcctacgggc cggagcagtt
      841 ccggaaggtc gcccgcgctc ttgactaccc ggtgctcgcc gataactgct acgacgacgc
      901 aacgggcgat ctcatctttc ccccttacgc tgtctgcgag accgacgatc tccaggtggg
      961 tgtcatcggg atagcggcca cgatcgtcga taaggtgatg ccgccgtctt tctcggaggg
     1021 tatccacttc accctgggga acgccgaact cccggagcat atcgcccggc ttcgcgacga
     1081 tgagggagtg gatatcgtcg tggtgatgtc gcacctcggt tttcccaagg aggtgaagct
     1141 tgcccgggag gtggagggta tcgatatcct cctctcgggg cacacccacg accgcctctt
     1201 tgagccggcc atcgtcaacg ataccatcat catacagtcc ggttgccacg gctccttcct
     1261 cgggcggctc gatcttacgg tcgaggaccg gcgggtgacg aagttcgatc acgaactcat
     1321 cgtcgtcgat gagggagtcc ggcccgagcc cggggttgaa gagatggtcg agggtatcat
     1381 ggaaccccac cgcgaacgcc tctcccgggt cgtcggggag acgcggacgg ggctcaaccg
     1441 gaatacggct atcgaggcca cgatggacaa cttcctcctc caggcgctcc tcgactgcac
     1501 cggcgcggat atggcgttct caaacggctg gcgctacggc gccccggtgc cgccgggtcc
     1561 ggtcacggta aacgacctct ggaacatcat cccgacaaac cccccggtat cgacggtcga
     1621 tatcacgggc cgggaactcc ggacgatgat ggaggaggac ctggagcata catttgcgcg
     1681 ggacccttac aaccagatgg gcggctacgt gaagcgctgt atgggggtca ccctctactg
     1741 cagactcgag aacgcgcccg ggctccgcat cgaggatttc tttgtcgggg gaaagaggct
     1801 tgacctggat gccgcctacc gggccgcgtt cgtcaccacc cagggcgtgc cggagcgtta
     1861 tgggaggaac cgggaagatc tcgataccct cgcggtcgag gcgctcgagc gttacctggc
     1921 aagagggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa aaagtatctt
     2041 atgcgatcag gacagcgttg acgacgccgt cctgaccggg tctgctgacg atccgtgcgc
     2101 gcccaacatc cgtcctgatg acggcgcctt tggtcaggag gctccgccgg acgtagttcg
     2161 ggttggcgct gtttgcctcg accgcctcga tcttcgcctt gtgggtctcg ccggttgccg
     2221 ggtttgcgac cgtcgcgtag tccaggcgga gcgctcgaac cttctggttg cccccgtagg
     2281 tccggacgac cttcttgcgc tcttcaccga tgtgcgtctc tgccggagct ctgccgatct
     2341 ccgatctctt cttgccctgg gaggtgtggt agcgtccgcc cgatggcttc cgtactgatc
     2401 ttccttgcca ctgcatgtga ccaccgatat actctgatga ggacgcgata cccgtcgcgg
     2461 gaaagcaatc tctcgttaaa gtgctataac tattcggggc gggaatattt aactatgctg
     2521 atcacgcgag gatcgcgtcc agcagttccc ggatcccttc cccggtcttc atgttcgtcc
     2581 ggaagatctg catctccggg ttgtagcggc gcatgtcgcg ttccatccgg tcgaggtcgg
     2641 cgccgacaaa cggagcgagg tcgaccttgt tgatgacgcc gatgttactg ccccgaaaca
     2701 tcatcgggtg cttgttcacc acatcgtccc cctccgtcga gctgatgacg acgatcctct
     2761 tctcggcgcc cagccggaag tcggtcgggc agatcatgtt gccgacgttc tcgatgaaga
     2821 ggatgtcgat atcgtcaagc ggcaggtgtt ctatggcgtg ctccaccagg tgggcgtcga
     2881 ggtgacactc cttcccggtg ttcacgttgc aggccggaac cccgagagaa acgatccgct
     2941 taaaatcatc gtcaccgtag acgtctcctg cgatggcgcc ggcgcggatc ccccgctccc
     3001 taagaagcgg gacgagtcgc tcgattgtgg cggtcttccc cgacccaatc gcgccgagga
     3061 ggtcgaacgc ccggatgccg tgctccctga gatgttcggc gttggcgtcc gcgatgcggt
     3121 tgttgacgtc gtagatgtcc ttctccacat ggacgtcgat atggtgcatg gtaagtaatg
     3181 tgatcatgat ctgtttatag gttaacaccc aacccctacg gcaatgagcg ctgaacgcat
     3241 cctgtatccc tgttacttcg acggcacgct gcagcggcgg gagggccgcc gggtctcgag
     3301 aaacctcggc gtgaagtcgc cggatctccc cgccatcgag accgccttgc ggaagatgaa
     3361 ggttccgtac cgggtggagg cgcaccacca ccccgcctgg tgggcggagc gcgaaggccg
     3421 ggttgcggtg gagtggaaag ggagtaagga agacctgatt cggaaggttg cccgcgagat
     3481 tggccggaag tgaccccgat gtacgacctg cacactcaca ccctcctctc cgacggcgaa
     3541 ctcctcccga cggaactcgt ccggcgggct gccgtcctcg gctataagac tctcgccatc
     3601 accgaccatg cggatgcctc gaacctcgtg catctggtgg atgcggtgag catggtccgg
     3661 gatgcggcgc gatggtacgg cgtggacctg ctcgtcggtg tggaactcac ccatgtcccg
     3721 ccgtccttga tcccggcgct cgcgcgcgag gcgaaagagc gcggtgccga tatcgtcgtg
     3781 gtccatgggg agacggtgat ggagccggtc gcgcccggga ccaaccgcgc cgcctgcacc
     3841 tgcgagtacg tcgacgtgct cggccacccc gggctgataa cggtcgagga tgcccgcgcg
     3901 gctgcagagc acggtgtcgc gcttgagatc acctcccgtg cggggcataa ccggacaaac
     3961 ggccacgtgg tgggtgtggc acgggaggcc ggctgtctcc tcacggtcga ttccgatacg
     4021 catgcaccgt ccgatctgat ggcagagggg gcgcggtggg aggttgcgct cggcgcgggg
     4081 ctgacggagg cggaatcacg ggatgtactc tcccgggatg tgaaacggtt tcttcagaag
     4141 taagatggag tatttatatt ttagcgaaac gtttttataa tcttatgttc aatatatata
     4201 cgttactaaa tatatccagc tagagtgttc cctagaggtt gcagtttgcg gttaatcggt
     4261 cgttctgtaa gtgtttgcgg taatcgcttg ttaatcttgc gatgtgatgc tgcgcagttg
     4321 ccccgcctct acggtgaggc agtggatcgc cggttaaaac ccatagggaa ggttgtcgat
     4381 atctttggga atatatcgtc tccctatgcc gtagtcctct gctacaacgg ctgtcccgtg
     4441 caggttggcg agaagatctt tgcaaaatag gtgtataatc gatggcagaa gttgaaaaat
     4501 taaagcagct gcagttgcag cgtgaggccc tgaagaagag aggggagcag aaggttaaag
     4561 agaccgagaa gaagcgcacc gaggagagtg tccagtccgt ctgtcccgag tgcggcagcc
     4621 gccagctcgt ccacgattac gagcgtgccg aactcgtatg ccagagctgc gggcttgtcc
     4681 tcgatgaaga gttcatcgat cgcggtccgg agtggcgtgc attcgaccac gatcagcgga
     4741 tgaagcgctc ccgtgtcggc gcaccgatga cgttcacgat ccacgacaag ggtctctcga
     4801 cgatgatcga ctggaggaac cgtgactcct acggtcgtgc gatctcgagc aagaaccgtg
     4861 cccagctcta ccggctccgg aagtggcagc ggcgtatccg tgtctcgaac gcgaccgagc
     4921 ggaacctcgc gttcgctctc tccgagctgg accggatggc gtctgcgctc ggcctgcccc
     4981 ggaacgtgcg cgagaccgcc gcggtggtct accgcgatgc ggtggacaag aacctgatcc
     5041 gcggccggag tatcgagggt gttgccgcgg cggcgctgta tgcggcgtgc cggcagtgca
     5101 gcgtgccccg gacgctcgac gagatcgcgg aggtctcccg tgtatcgagg aaggagatcg
     5161 ggcgcaccta ccggttcata tcgcgcgagc tcgggttgaa gcttctgccg acgtccccga
     5221 tcgactacgt tccgcgcttc tgctcgggcc tgaacctgaa gggtgaggtc cagagccggg
     5281 cagtcgagat cctccggcag gccggagaac gcgaacttac gagcggcaga ggcccaacgg
     5341 gcgtcgctgc ggccgctatt tacatatcat cgatcctcgg cggcgagcgg cgcacccagc
     5401 gcgaggtcgc cgaggtcgcg ggcgtgaccg aggtcacgat caggaacaga tataaggaac
     5461 tggcagaaaa attagatatc gagatcatac tctgatctca tatcc
//
LOCUS       DAOSII010000116         5459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_13410_length_5459_cov_47.883975, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000116 DAOSII010000000
VERSION     DAOSII010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5459
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13410_length_5459_cov_47.883975"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..1120)
                     /gene="argF"
                     /locus_tag="PLV90_08330"
     CDS             complement(191..1120)
                     /gene="argF"
                     /locus_tag="PLV90_08330"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOF97161.1"
                     /translation="MKKDFLSITDIDGYELESIVADAMYLKRLKSTGTPHEFLRGKSL
                     GMIFEKASTRTRVSFEVGMTDFGGHALFLNPADLQLGRGEAIKDTARTLARYTDAVMI
                     RAYSHATVEEFARYSTVPVVNGLSDRLHPCQVLSDIMTLNERFGDLQDLKLAWVGDGN
                     NVCNTWLLSAAMTGMEIVVASPPRYRPRDAVVDQARAAGGRITIVADPAEAVRDADVL
                     YTDIWVSMGDEKERTERMQALRDYTVDSRLLARAPPDALVMHCLPAHRGEEITDEVID
                     GPQSIVWDQAENRLHAQKALLLRLIAGGMPATE"
     gene            complement(1117..2409)
                     /gene="purD"
                     /locus_tag="PLV90_08335"
     CDS             complement(1117..2409)
                     /gene="purD"
                     /locus_tag="PLV90_08335"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOF97162.1"
                     /translation="MVMKLLVVGGGGREHAITRALSCNSDVKLFSVMARENPGIARLA
                     ERVFLTDETDLPGIVRFAMENGIDVAVIGPEAPLEAGVVDHLEAAGIPCVGPTRAAAR
                     IETDKAFCRRLMERHGIAGCPEYRVFQDPEEAGRFIEAYGGDLAVKPIGLTGGKGVRI
                     MGEHVDVAGAVGYVREIGGDVVLEERLIGEEFTLQAFVDGKHLVPMPLVQDHKRAYEG
                     DVGPNTGGMGSYSLSDHMLPFVTRRDYEEALRIMEDTVAAMRDEGTPYRGILYGQFMN
                     TRDGPKVIEFNARFGDPEAMNVLSILESDLAGIVRHIVEGDLSPAHVRFAHKATVCKY
                     IVPEGYPDAPMAGEPLAPGDCGDALLYYANVEERDGVLYTRTSRTLAFVGRGETLEEA
                     EAIAERAASSVTGRVFHRRDIGTQEVLERRCRHMKEVS"
     gene            complement(2435..2947)
                     /gene="pyrE"
                     /locus_tag="PLV90_08340"
     CDS             complement(2435..2947)
                     /gene="pyrE"
                     /locus_tag="PLV90_08340"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOF97163.1"
                     /translation="MVNPIAKLLLESGAVEFGDFVLASGAPSSYYIDIKAATTNPTIL
                     AAIGKAVAEGWDFDTVAGVAVGGIPIAVAVSLASGRPYAIVRKEEKDHGRAGTIIGDV
                     AGRNVLLVEDVTTSGGSVLYGLAALRAAGARVDRVVTVVDREAGAHEALAEKGTSLFA
                     LVRVSELLNG"
     gene            complement(2941..3441)
                     /locus_tag="PLV90_08345"
     CDS             complement(2941..3441)
                     /locus_tag="PLV90_08345"
                     /EC_number="2.7.7.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
                     synthase"
                     /protein_id="HOF97164.1"
                     /translation="MLPAYVPNSAAALFGGGTPIDSGRTLGDGRRIFGDGKTYRGFFG
                     GVLAGVLVGLVEILAWSSFGLTALPHQTILSVTLFATGALLGDLAKSFLKRRLGKERG
                     ESWFLADQYDLVIGSFLLILLVYPEWLFENITLPIAVWIVVMTPLLHRAVNIIGYYIG
                     VKEVPW"
     gene            3583..5052
                     /locus_tag="PLV90_08350"
     CDS             3583..5052
                     /locus_tag="PLV90_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOF97165.1"
                     /translation="MLMKKTKSLCPTCGRVLDADIVEEEGKVWLVRTCPVHGEYKALY
                     WSDADMYRRFEEYERIGRGVANPQKTTSPAGCPNDCGLCPNHRSATLLANIDLTNRCN
                     LNCDFCFANAQACGYIYEPTFDQIVDMLRMLREEKPVPVPAVQFSGGEPTMRDDLPEL
                     VLKAKELGLYQVQVATNGIRIAQEPGYAARLKEAGLSTVYLHFDGVTRETNSKLASDR
                     RAIENCEKIGLGVVLVPTIINGRNDHEVGAIIRYAAEHIKTVRGVNFQPVAFTGAASE
                     DDVRKERITIPELADRIEEQTDGVIKKEYFYPVPCVVPISELVEAYTGKPQVTFTTHQ
                     HCGAATYVFITDEGMVPINKMVDVDAFFESVEKMGANLAKGGSLNKYVTLVEGVKDLY
                     VSTRKAEQTNTGELLKLIGRTLIMQNFEALREFHWNALFIGTMHFMDNYNYDISRVQR
                     CCIHYATPDGRLIPFCTYNSGPVYREQVWKAFALPPEDE"
     gene            complement(5134..>5459)
                     /locus_tag="PLV90_08355"
     CDS             complement(5134..>5459)
                     /locus_tag="PLV90_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867831.1"
                     /note="catalyzes the interconversion of alanine and
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HOF97166.1"
                     /translation="EGTHINAIGADAPGKQELDPRILTGAEVFVDDPGQAAHSGEINV
                     PIETGLYDAGMIAGTLGEVVIGKRGRSSPGAVTVFDSTGLAIQDLAIAALIIERDDGY
                     DLPFP"
BASE COUNT         1088 a         1778 c         1626 g          967 t
ORIGIN      
        1 cgatatcagg tgggccgccg tgcacaggat catggtgacc accgccacgg ggggctgcca
       61 cggcgcgcgc gcgccccgcc ggcgggtcgc gggcatcgct gccggcgcgt tgagaaaggg
      121 cctgatcggt atagccggcg acctcatgcc ttcgccgccg gaaccctgaa aaaagatact
      181 cttcccgccg tcactccgtc gcaggcatgc cgccggcgat cagccgcagg agcagtgctt
      241 tctgcgcgtg gagccggttc tcggcctggt cccagacgat gctctgcggc ccgtctatca
      301 cctcgtcggt gatctcctct ccgcggtggg ccgggaggca gtgcatcacg agcgcgtcgg
      361 gcggtgcccg ggcaagaagg cgggagtcta ccgtgtaatc cctgagggcc tgcatgcgct
      421 cggtgcgctc cttctcgtcg cccatggaga cccatatgtc ggtatagaga acatccgcat
      481 cccggacggc ttccgccggg tccgcgacga tcgtgatcct gccgcccgcc gcccgcgcct
      541 ggtcgacgac ggcatccctg ggccggtaac gcggggggct tgcgacaacg atctccatcc
      601 cggtcatggc cgcggagagg agccacgtgt tgcagacgtt gttgccgtct ccgacccatg
      661 ccagcttcag gtcctgcagg tcgccgaacc gctcattgag ggtcatgatg tcggagagca
      721 cctggcaggg gtgcagccgg tcggacaacc cgttgacgac cgggaccgtc gagtagcggg
      781 caaactcctc gacggtggca tggctgtagg cacggatcat caccgcatcg gtgtagcggg
      841 caagcgtccg cgccgtatcc ttgatcgctt cgccccgccc aagctgcaga tccgcggggt
      901 tcaagaagag cgcgtggccc ccgaaatcgg tcataccgac ctcaaacgag acccgggtgc
      961 gggtggacgc cttctcgaat atcatcccga ggctctttcc cctcaggaac tcatgcgggg
     1021 tccccgtgga cttcagccgc tttaagtaca tggcatcggc aacgatgctc tccagttcat
     1081 acccgtcgat atcagtgatc gagagaaaat ccttcttcat gaaacctcct tcatatgccg
     1141 gcaccgccgt tcgagaacct cctgcgtgcc gatatcccgg cggtgaaaga cgcgcccggt
     1201 aacagatgat gccgccctct cggcgatcgc ctcggcctct tcgagcgtct cgcccctccc
     1261 gacgaacgcg agcgtccgcg acgtccgggt gtagagcacc ccgtctcgct cctcgacgtt
     1321 tgcgtagtag aggagggcgt cgccgcagtc gccgggggcg agaggctcgc cggccatcgg
     1381 ggcatccggg tagccctcgg ggacgatata cttgcagacc gtcgccttat gggcgaaccg
     1441 gacatgcgcc ggcgagagat cgccctcgac gatgtgccgg acaatcccgg cgagatcgga
     1501 ctccaggatc gagagaacgt tcatcgcctc cggatcaccg aatcgggcgt tgaactcgat
     1561 gaccttcggg ccgtcgcggg tgttcatgaa ctggccgtag aggatccccc ggtacggcgt
     1621 cccctcgtcc cgcatggccg cgaccgtatc ctccatgatc cggagcgctt cctcgtaatc
     1681 cctccgggtg acgaagggaa gcatgtggtc ggagagggag tacgaaccca taccgccggt
     1741 gttcggcccc acgtcaccct cgtatgctcg tttgtggtcc tgaacgagcg gcatgggcac
     1801 caggtgcttg ccgtcgacga acgcctggag ggtgaactct tcaccgatga gccgctcctc
     1861 cagcacgaca tcccccccga tctcccggac gtacccgacc gctcccgcca cgtcgacgtg
     1921 ttcgcccatg atccgcacac ccttcccccc ggtgagcccg atgggcttga cggcgagatc
     1981 gccgccgtag gcctcgatga accgcccggc ctcctccgga tcctgaaaga cccggtactc
     2041 cgggcatccc gcgataccgt gccgctccat cagccggcgg cagaacgcct tatccgtctc
     2101 gatacgagcc gcggcccggg tcggccccac gcagggtatc cccgccgcct cgaggtggtc
     2161 gacgacgcct gcctcaaggg gcgcctccgg cccgatgacc gcaacatcga tcccgttctc
     2221 catggcgaac cggacgatac ccgggagatc ggtctcatcg gtgagaaaca cccgctccgc
     2281 gagccgggca attcccgggt tctctcgtgc catgaccgag aagagcttca cgtcactgtt
     2341 gcaggaaagc gccctggtga tagcatgctc cctgccacca ccccccacaa cgagtagttt
     2401 cataaccatc tatttgcccg ctcttaaaaa atgtttatcc gttcagcagc tcgctgaccc
     2461 gcacaagtgc gaagagcgac gtccctttct ctgcgagcgc ctcgtgggcg cccgcctccc
     2521 ggtcgacgac ggtcacgacc cggtcgacac gggcacccgc ggcacgcagg gccgcaaggc
     2581 cgtagagaac gcttcccccc gatgtggtca cgtcctcgac gagcagaacg ttcctgccgg
     2641 ccacgtcgcc gatgatcgtc ccggccctgc cgtgatcctt ctcctccttc ctgacgatcg
     2701 cgtagggccg gccgctcgca agcgagacgg cgacggcgat cgggattccg ccgaccgcca
     2761 ccccggccac cgtatcaaaa tcccatccct cagcgacagc ctttcctatc gccgcaagga
     2821 tcgtggggtt cgtcgttgcg gccttgatgt cgatgtagta ggagctcggg gctcccgatg
     2881 cgaggacaaa gtccccgaac tcaacggccc cgctctccag caacagtttt gcgattgggt
     2941 tcaccatggt acctctttaa ctccgatgta ataaccgata atgttcaccg cccggtgaag
     3001 gaggggtgtc atgacgacga tccagacggc aatgggtaaa gtaatattct caaagagcca
     3061 ctccggataa accaggagga tgagcaggaa tgaaccgata accagatcgt actggtcggc
     3121 caggaaccag gactcgcccc gctccttgcc cagcctccgt ttcaggaagc tcttggcgag
     3181 atcgccaaga agcgcgcctg ttgcaaacaa cgtaacggag aggatggtct gatggggaag
     3241 tgcggtcagg ccgaacgagg accatgccag gatctcgacg aggccgacaa gcacgccggc
     3301 aagtacaccc ccgaagaatc cccggtatgt cttcccgtcg ccaaatatcc gcctcccatc
     3361 gccaagcgtc ctgccggagt cgataggtgt tccgccgccg aagagcgctg ccgctgagtt
     3421 cggcacgtag gccgggagca tgatccacag cgccgcgagc gcagggaaga tccaggagcc
     3481 gatgtctgcg tcgatcaatg tcaatattca gtacaatcag taattataaa gattaaagtg
     3541 tcgaaatatg agataggaat gctagatggg cacggtgaga gaatgctgat gaaaaagaca
     3601 aaaagcctct gtccgacgtg cggccgcgtg ctcgatgccg atatcgtcga ggaggagggg
     3661 aaggtctggc tggtccgcac ctgtcctgtt cacggcgagt acaaggccct ctactggtcg
     3721 gatgcggaca tgtaccggag gtttgaggag tacgagcgca ttggaagggg ggtggcaaac
     3781 ccacagaaga ccacttctcc agcagggtgc ccaaacgact gcggcctctg cccgaaccac
     3841 cggtcggcga cattgcttgc aaacatcgac ctgacgaacc ggtgtaacct caactgtgac
     3901 ttctgttttg caaacgccca ggcatgcggc tacatctacg aaccgacctt cgaccagatc
     3961 gtcgatatgc tgcgtatgct gcgcgaggaa aaaccggtcc cggtccctgc cgtccagttc
     4021 tcgggcggcg aaccgacgat gcgcgacgat ctccccgaac ttgtattaaa ggcaaaggag
     4081 ctcggcctct accaggtgca ggtcgcaacg aacggcatcc ggatagcgca ggaacccggc
     4141 tacgctgcac gactgaagga ggcagggctc tccaccgtct acctgcactt cgacggcgta
     4201 accagagaga ccaattcaaa actcgcaagc gaccggcggg ccatagagaa ctgcgagaag
     4261 atcggtcttg gcgtggtgct ggttcccacg atcatcaatg gccgaaacga ccacgaggtg
     4321 ggcgccatca tccggtatgc cgcagagcat atcaagaccg tgagaggcgt caacttccag
     4381 ccggtggcgt tcaccggggc cgcaagcgag gatgatgtca ggaaagagcg tatcaccatc
     4441 cctgaacttg cggatcggat cgaggagcag acggacggcg tgatcaagaa ggaatacttc
     4501 tatccggtgc cctgtgtcgt cccgatatcc gaactcgtcg aggcatatac gggcaaaccc
     4561 caggtcacgt tcaccacgca ccagcactgc ggcgcggcga cctacgtctt catcaccgac
     4621 gaggggatgg tcccgatcaa caagatggtg gacgtcgacg ccttctttga gtctgtcgag
     4681 aagatggggg cgaatctcgc caaaggcggg tcgctcaaca agtacgtgac cctggtcgag
     4741 ggcgtcaagg atctctacgt ctccacgcgg aaggcggagc agacgaatac cggggagctc
     4801 ctgaaactga tcggcagaac cctcatcatg cagaacttcg aggccctgcg cgagttccac
     4861 tggaacgccc tcttcatcgg gacgatgcac ttcatggaca actacaacta cgacatatcc
     4921 cgggtgcagc ggtgctgcat ccactacgcg acccccgacg gccgcctgat accgttctgc
     4981 acctacaaca gcggccccgt ctaccgcgaa caggtctgga aagccttcgc cctgccgccc
     5041 gaggacgagt aacggcccat cataccgcaa aagagaggtc cgccggccct ctcccggccc
     5101 cttttctcga taagcacgat ttatctgtga agatcagggg aacggcaggt catacccatc
     5161 atccctctcg atgatgaggg cggcgatggc aaggtcctgt atggcaagtc cggtggagtc
     5221 aaagaccgtg acggcgccgg gagacgaccg tcccctcttc ccgatgacga cctctccgag
     5281 ggttcccgct atcatacccg cgtcgtagag gccggtctct atcgggacgt tgatctcgcc
     5341 tgagtgggca gcctggccgg gatcgtcgac aaaaacctcc gccccggtca gtatccgggg
     5401 atccagttcc tgtttgccgg gagcatcggc gccgatcgcg ttgatatgcg tcccctccc
//
LOCUS       DAOSII010000117         5388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_13607_length_5388_cov_45.070879, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000117 DAOSII010000000
VERSION     DAOSII010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13607_length_5388_cov_45.070879"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..149
                     /gene="cpsB"
                     /locus_tag="PLV90_08360"
     CDS             <1..149
                     /gene="cpsB"
                     /locus_tag="PLV90_08360"
                     /EC_number="2.7.7.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867012.1"
                     /note="capsular polysaccharide colanic acid biosynthesis
                     protein; catalyzes the formation of GDP-mannose from GTP
                     and alpha-D-mannose 1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HOF97167.1"
                     /translation="GESTFVPAGTVHRLANPGLLPLELIEVQIGEYTGEDDIVRFEDD
                     FERA"
     gene            259..331
                     /locus_tag="PLV90_08365"
     tRNA            259..331
                     /locus_tag="PLV90_08365"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:293..295,aa:Gln,seq:ttg)
     gene            551..1843
                     /gene="eno"
                     /locus_tag="PLV90_08370"
     CDS             551..1843
                     /gene="eno"
                     /locus_tag="PLV90_08370"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOF97168.1"
                     /translation="MNEVDTRIRSIHAREILDSRGNPTVEADVTLAGGAFGRAATPSG
                     ASTGRHEAVELRDGVKDRYGGKGVQQPVARVNGEIAVTLQGMDAREQAAVDEALSLLD
                     GTPNKRNLGANATLAVSMAVARAAAAAEGVWLWEYLGDPLKPLLPVPMMNILNGGIHA
                     GWQGPDFQEYMIAPYGAPSVTEAIRWGSETYHALKDILAAQGYTTGVGDEGGFAPAVS
                     TNTEPLDLIVEAIGRAGYTPGRDIGIALDPASSAFYSNGVYNLRTEGLTLTAAEMVDR
                     YRDLVAAYPIIVIEDGLAEDDWDGWRLLTRAIGDRVELVGDDLFVTNVERIEMGIRKG
                     AANAVLIKPNQIGTVTETIAAVRLAQQQHWGAMVSHRSGETVDTFIADFAVAMQTGHI
                     KAGAPARGERVAKYNQLARIEEAAGGTARYAGRGGFVR"
     gene            complement(1856..3256)
                     /locus_tag="PLV90_08375"
     CDS             complement(1856..3256)
                     /locus_tag="PLV90_08375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF97169.1"
                     /translation="MEQQVQVVIPNDPAFIAVIRLAVSRMASLAGFPAGTIEEITSGV
                     EEAVRWIVDHAFPPGVASDITLTCAIHADRFEVRIVEHGIPFDPETASPALPRCRAVM
                     DRIAFTNRGWAGKETLLAKDCRESAHPSGSPVPPASSGPVLVRPTLPDEAVEISRCAY
                     FSYGYSYGYERVYHPDVLKALIRSGDLISFVAVDENTVIGHAGLLFCDDPGVAEIGLG
                     FVNPPYRSRRVFSDLMTTVVAEAERRHLVALSGQAVCTHLYSQRSARTFGMGECALLL
                     SRYTPLRFTGIADENQVRESILAVFRYISPPEHPVLYPPKHHAGILREIYDRLGVHPP
                     FTTDNASTDLPEKSEYSLTVEEIYRTAQISVRSIGTDLIPRLRRELARLRAGRLEAVY
                     IHLPLTAPKSAATVDAIENLGFFFAGLHLASTGEDLLTLQYLNNQILDYDTVTVASPH
                     AGRLKEYIRERDPWQG"
     gene            3579..4766
                     /locus_tag="PLV90_08380"
     CDS             3579..4766
                     /locus_tag="PLV90_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOF97170.1"
                     /translation="MESAALSIEFQMSLLLFLSLAGYLLASRINQSATIGAILVGVLV
                     GPSVLGLITYTDFVQSLAHLGAIILLFVIGFEFNIRDILDLRYGVIGLVGVIIPWIGG
                     YATAVLFGLDFPSAVFIGTALTATSIAITANVLKEIGVLQTGAARAIIGAAVIDDILS
                     LLVLAVATDLVVSGDVSATSVVLMLVKAVGFIVVAGAVGYFGIRKAIQRMDATPLAQK
                     YPEFVFIFAMMVAFLYAMSASFVGLSGIIGAFLAGVALAGVELRQSKGVYEGADYFQI
                     VFASIFFVSLGILADIRALTSDMVLFLAALTVVAIVTKVVGCGLPARLMGMCREDSLI
                     VGFGMAPRGEVAMIVALIGLDSGLIGQSVFVVIVLMSLLTTLITPIVYRNWFFRGAYC
                     AVE"
     gene            complement(4849..5304)
                     /locus_tag="PLV90_08385"
     CDS             complement(4849..5304)
                     /locus_tag="PLV90_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOF97171.1"
                     /translation="MDVEEYRSIISNAIEREIEAYTFYRMIHERVTDENLKGLFNELA
                     GEESKHRRTLEALLLREPEKLGFDTTRDYKVADALETPPLSADLKPLDGLVIAIRKEL
                     DAMQLYTQLAGLSVDPEQIELFESLAAMERGHKARLEDIYTNMAFPEVW"
BASE COUNT          922 a         1694 c         1760 g         1012 t
ORIGIN      
        1 acggcgagtc caccttcgtc ccggcgggga cggttcaccg tctcgcgaac cccggcctcc
       61 tgccgctcga actgatcgag gtgcagatcg gggagtacac cggcgaggac gacatcgtca
      121 ggttcgaaga cgacttcgag cgggcgtgag cgatggatat tccacccccc tgcccgcgac
      181 caaatcgata taatcccggg atgcatagta cataagggcc ggtaccccag gtatcagcga
      241 ttggattcgt ttctcttgac ccccgtagtg tagtggtcaa tcatgcagga ctttggatcc
      301 ggcgacggcg gttcgaatcc gcccgggggt atcatgacac ggagttcggt accgcggtat
      361 tacgccggcc tctcctgttc ccctccttcc ggcctccgta catccgcccc gatagtccgc
      421 gatgttcggt aggcccatcc gggagttcag tacgcaaacg cctcatgttg ccgccgccgc
      481 ccggacacgc cccgggccgc cgataccttt tttgcgctcg ataccctccc gccccccggt
      541 gccatcgaac atgaacgagg ttgacacgag gatacgatcg atccatgccc gcgagatact
      601 ggactcaagg gggaacccga ccgtcgaggc ggacgtcacg ctcgccggcg gggcgttcgg
      661 gcgcgccgcg accccatccg gcgcatcgac cggaagacac gaggccgtcg agctccgtga
      721 cggcgtaaaa gaccgttacg gcggcaaggg ggtgcagcaa cccgtcgccc gggtcaacgg
      781 cgagattgcc gttaccctgc agggcatgga tgcccgcgag caggcggcgg tggacgaggc
      841 cctctctttg cttgatggaa cgccgaacaa gcggaacctg ggcgccaacg ccacgctcgc
      901 ggtctcgatg gccgtcgccc gtgcggccgc cgcagcggag ggtgtctggc tctgggagta
      961 cctgggagac cccttaaagc cgctcctccc cgttcccatg atgaacatcc tcaacggcgg
     1021 catccacgcc ggctggcagg ggccggactt ccaggagtac atgatcgcgc cctacggcgc
     1081 cccgagcgtc accgaagcta tccggtgggg gagcgagacc taccatgcgc tcaaggatat
     1141 actcgcggca cagggctaca cgaccggcgt cggcgatgaa ggcgggtttg cccccgcagt
     1201 ctccacgaac accgaaccgc tcgacctgat cgtggaggcg atcgggcgcg ccgggtatac
     1261 ccccggcagg gatatcggga tcgccctcga cccggcatcg agcgcgttct actcaaacgg
     1321 cgtctacaat ctcaggaccg aaggtctgac cctgaccgcc gcggagatgg tggaccgcta
     1381 ccgcgacctc gtcgcggcat acccgatcat cgtcatcgag gacggccttg ccgaggacga
     1441 ctgggacggc tggcggctgc tgaccagagc catcggcgat cgggtggaac tcgtcggcga
     1501 cgatctcttc gtcacgaacg tcgaacggat cgagatgggt atccggaagg gggccgcaaa
     1561 cgccgtcctg atcaagccca accagatcgg gacggtgacc gagacgatag ccgcggtgcg
     1621 tctcgcgcag cagcagcact ggggcgcgat ggtctcccac cggagcggcg agaccgtcga
     1681 cacgttcatc gccgacttcg ccgtcgccat gcagacgggc cacatcaagg ccggcgcgcc
     1741 ggcacggggc gaacgggtgg cgaagtacaa ccagctcgcg aggatcgagg aggcggccgg
     1801 aggtacggcc cggtacgccg gccggggcgg gtttgtccgg tagggcgggc atccttcacc
     1861 cctgccaggg gtcccgctcc cggatatact ccttcagccg gccggcatgg ggcgatgcca
     1921 ccgtgaccgt atcgtagtcc aggatctggt tgttgaggta ctggagggtg aggaggtcct
     1981 cgccggtgga ggcgaggtgc agaccggcaa agaagaagcc gagattctcg atcgcgtcca
     2041 ccgtcgcagc gctttttggg gcggtgagcg ggaggtggat gtagacggcc tcgaggcgtc
     2101 cggcacggag gcgggcaagt tcgcgcctga gccggggtat aagatcggtg ccgatggatc
     2161 ggacgctgat ctgggccgtc cggtaaatct cttcgacggt gagagagtat tcgctcttct
     2221 ccggaagatc ggtcgacgca ttatcggtgg tgaagggcgg gtgcacgccg aggcgatcat
     2281 agatctctcg cagtatgccg gcatggtgct ttggcgggta gaggaccgga tgctccggcg
     2341 gcgagatgta gcgaaagact gcaaggatgc tctccctgac ctggttctcg tcggctatgc
     2401 cggtaaaccg gagcggtgtg taacgggaga ggaggagggc gcactcgccc atcccgaatg
     2461 tccgggccga ccgttgcgag tagaggtggg tgcagaccgc ctgcccggag agcgctacca
     2521 ggtgccggcg ttcggcctcc gcgacgacgg ttgtcatcag gtccgagaag acccgccggc
     2581 tccggtaggg cgggttcacg aacccgagcc cgatctcggc cacaccgggg tcgtcgcaga
     2641 agaggagtcc ggcatggccg atcacggtat tctcgtcgac cgcgacgaac gatataaggt
     2701 cgcccgaccg gatcagggct ttgagcacgt ccgggtggta gaccctctcg tagccgtagg
     2761 agtatccgta actgaagtag gcgcaccgcg agatctccac cgcttcgtcg ggcagggtcg
     2821 ggcggaccag cacggggccg gaggaggcgg gcggcaccgg agagccggag gggtgtgcgc
     2881 tctccctgca gtccttcgcc agcagagtct ccttcccggc ccaacccctg ttcgtgaagg
     2941 cgatccggtc catgaccgcc cggcaccggg ggagcgccgg ggaggctgtc tccgggtcga
     3001 aggggatccc gtgctcgacg atccgcacct caaaccgatc ggcgtgaata gcgcaggtga
     3061 gcgtgatatc gctcgccacg cccggcggga aggcatgatc gacgatccag cgcaccgcct
     3121 cctcgacgcc ggaggttatc tcctcgatcg tgccggcggg aaagccggca agggatgcca
     3181 tccgcgagac cgccagccgg ataaccgcaa taaacgccgg atcgttcggg atgaccacct
     3241 gtacctgctg ctccatccgt agtggaggtt tacagtgggc ggtcttaaag atagtgtact
     3301 gagaacggac cccgggcgcg ggttatcggg aagtgcgttc ccgcgggaga gggggagggt
     3361 cctattgatc tgatggttac tgttcccacc cggatgcgat cgagcatggc cgacatagct
     3421 gtgggggtga gccggagaac ccgccgttcg gtatcctctt cccgcccgga ctgttcagcc
     3481 ggggtggtat gttgctgttt gccctcccga tactgttctg caactcatgc gcggtgcaga
     3541 gatttcttaa ggcctcccct cccagatctg ggataacgat ggaaagcgcc gctctatcga
     3601 tcgagttcca gatgagtctc ctcctcttcc tctctctcgc cggttacctc ctggcgtccc
     3661 ggatcaacca gtcggcgacg atcggggcca ttctcgtggg ggtccttgtg ggaccgagcg
     3721 tgctcggcct gatcacctac acggacttcg tccagagcct ggcgcacctc ggggcgatca
     3781 tcctcctctt cgtcataggg ttcgagttca acatcaggga tatcctcgac ctgcgatatg
     3841 gggtcatcgg ccttgtcggc gtcatcatac cgtggatagg cggctatgcc accgccgtcc
     3901 tcttcgggct cgactttccg agcgcggtct tcatcggcac ggcgctgacg gcgacgagca
     3961 tcgccatcac ggcaaacgtc ctcaaggaga taggcgtcct ccagaccggg gcggcgcggg
     4021 cgatcatcgg tgccgcggtc atcgacgaca tcctctccct cctcgtcctc gccgtcgcca
     4081 ccgacctcgt cgtgagcggg gacgtctcgg caacatcggt cgtcctgatg ctcgtgaaag
     4141 ccgtggggtt catcgtcgtt gccggcgccg tcggttactt cggtatacga aaggccatcc
     4201 agcggatgga cgcaactccc ctggcgcaga agtatcctga gttcgtcttc atattcgcca
     4261 tgatggtggc gttcctgtat gcgatgtccg caagcttcgt gggcttgtcc ggcatcatcg
     4321 gcgcattcct cgccggggtc gcccttgccg gcgtcgagct ccggcagagc aagggcgtct
     4381 acgagggtgc cgactacttc cagatcgtct tcgcctcgat cttcttcgta tccctcggga
     4441 tcctcgcgga tatccgtgcg ctcacatccg acatggtcct cttcctcgca gcgctgaccg
     4501 ttgtcgccat cgtcaccaag gtcgtcgggt gcggcctccc cgcccggttg atggggatgt
     4561 gccgggagga ctccctcatc gtcgggttcg gtatggcgcc gcggggcgag gtggcgatga
     4621 tcgtcgccct gataggtctc gactcagggc tcatcggcca gagcgtcttc gtggtgatcg
     4681 tcctgatgag cctcctgacc acgctcatca cgccgatcgt ctacaggaac tggttcttca
     4741 ggggcgcata ctgcgccgtg gaatgatacg gaaggagaag atggccgggt gcctcccggc
     4801 ctggcacctc cccggtgcgg ggcggattgc cccccgggct atcccgcctc accagacttc
     4861 cgggaacgcc atgttcgtgt agatatcctc gaggcgcgcc ttatgtcccc gttccatagc
     4921 ggcgaggctc tcgaagagct cgatctgctc cgggtcgacg ctcagccccg cgagctgggt
     4981 gtagagctgc atcgcgtcga gttctttccg gatagcgatg acaaggccgt cgagcggctt
     5041 taagtcggcc gagagcggcg gagtttcgag ggcgtcggcg accttgtagt cccgcgtcgt
     5101 atcaaacccg agtttctccg gttctctcag gagaagagcc tcaagggtcc tccggtgttt
     5161 gctctcttcc ccggcaagct cgttgaagag accctttagg ttctcatccg tgaccctctc
     5221 gtggatcatg cgatagaacg tgtaggcctc gatctctctc tcgatggcat tcgatatgat
     5281 ggaacggtac tcttcgacat ccattgggta tacaccggcg ggagagtggc gtccgggact
     5341 attatacttt gtcagggccg gggtatggta tccgtcccac ctctttcc
//
LOCUS       DAOSII010000118         5358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_13699_length_5358_cov_40.318122, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000118 DAOSII010000000
VERSION     DAOSII010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13699_length_5358_cov_40.318122"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(243..857)
                     /locus_tag="PLV90_08390"
     CDS             complement(243..857)
                     /locus_tag="PLV90_08390"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HOF97172.1"
                     /translation="MPKKKKTFENVYQFKITLQEIRPPIWRRIQVPETYSIWDFHVAI
                     QDAMGWTDSHLHEFQMKDPKSHAEVEIGFPSEFAPGILLSWETAMADFFAINNPRAVY
                     TYDFGDDWRHMVILEKILPKEPGIRYPRCIKGKRACPPEDIGGVWGYEEYLEKLRNPD
                     ARDEDDEYCDFDFDPEHFSCAEVVFDDPEERLKQSGILDSLCID"
     gene            1550..2806
                     /locus_tag="PLV90_08395"
     CDS             1550..2806
                     /locus_tag="PLV90_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97173.1"
                     /translation="MLAFARLGDREAFARIWHDLIERDGLLEPVPAEEFIKYPGDYTL
                     LGEFPFREQIESLEYLFSYGVQENREAEVFAFIRSLPDYLESLSIQVDLEEREYGISR
                     CFAALPVIKAIAEGSLDVVEKILSTYDPISDERLLEETGENIERIRKSGVEAGVLNEL
                     LRWSVDPVQPAGKLLDTLRRHTGGDDEPILAMFDMANMGASGVAVGELASLLLEAHPD
                     NRCLIESVYGSLKSEERYDEALAFADRHEFLKQDDAQFESLKLEALRSSDEEAAFQFL
                     LGGIKERRMLERYADLFELALALDRMDEVASLRWIFAEEKGAIAGSHMLNALDRLKKR
                     DVKEGMALFERSKRSGFPEELALMLLARSLLSAGYPKRVVGLREKMLKTSLPPEEIYP
                     LLIRAYRELGRESEARDVEAMLEAVR"
     gene            complement(2931..4499)
                     /locus_tag="PLV90_08400"
     CDS             complement(2931..4499)
                     /locus_tag="PLV90_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOF97174.1"
                     /translation="MLPASAGLYTDSCGMVWTDAALPFERNNETAYTEELMASYGPIM
                     ANLTEAPDLSNMTWSDAFREACACMEERYAFTEWRGVDWDALYATYAQKIADAERSKD
                     TAAYYRTLREFIFAIPDGHVLVLSPDDFGAKHADIGGGYGFAVARLDSGEVIVTYVAS
                     GSEAERAGIRFGDEVVSWNGRPIGEAINATPILWTPIKPSTAEGILLQKQRFLARAPV
                     GTTTEIGIAGPTPRTVSLTATDDDYETLARTSIFLGRETNDGAAGSSAELKAMLTNET
                     VTTRTLPGGIAYIAVYEESYDVYQPFRAAMETAIAGGAPGIVIDLRFNRGGDDNLAAA
                     FAGWFVDRPVFYEYGTTYDPGTGEQAVLWESWSEPRPDRYEGPVALLVSPYTISSGEG
                     LPKVFAETGAGAIVSWYGTNGAFGMESMGPVLPLGIMTAFPQGASLDELGRIQVDSNR
                     SMIGGVAPTVRVPLDRDTLERAMAGEDVQLSYAVAWIEAQAGGEPAPTPKAAAGWVIP
                     LAAIIGLVVWFGRR"
     gene            complement(4688..5146)
                     /locus_tag="PLV90_08405"
     CDS             complement(4688..5146)
                     /locus_tag="PLV90_08405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF97175.1"
                     /translation="MQSDTMLIREAAREDLAAVLSLYAHLHDADEKAGDRVLERICLE
                     IPADPRTHLFILEYEGVPVATCVLHIHSNLTRGARPYGLIENVVTHREYRNRGFGTTL
                     LTHAPGYAWERNCYKVMLLTGRSEPNVFRLYEKAGFLRGVKEGLIAYPPE"
BASE COUNT         1014 a         1660 c         1619 g         1065 t
ORIGIN      
        1 cgacgatgaa cgaacggctg cggcagtact acgggagggt gcagcagcac ttcaagggcg
       61 ggctcccaaa agagatccag cctccggaat accagtaggg ggatatccgg gagaaacccc
      121 cggagaactc tttttgtgcg ggatatatcc cctcattatc gtcccggcaa ctttctgtcg
      181 ctcccgagaa cccctctccc ctgcccgatc gggagcgggg tcgcccccac gattatggca
      241 gatcaatcga tgcagaggct atccaggata ccactctgtt tcagcctctc ttcggggtcg
      301 tcaaagacaa cctccgcgca gctgaaatgt tcgggatcga aatcgaagtc gcagtactcg
      361 tcatcctcat cgcgtgcatc cggatttctg agtttctcca ggtactcctc gtatccccac
      421 acacccccga tgtcttcggg agggcatgcc cgctttccct tgatgcaccg gggatacctg
      481 atgccgggtt ctttcgggag gatcttctca aggatgacca tgtgcctcca gtcgtcgccg
      541 aaatcgtagg tatacaccgc ccggggatta tttatggcga agaagtcggc catggcggtc
      601 tcccagctga gcagtatccc gggagcgaat tcgcttggga acccgatctc cacctcggca
      661 tggctttttg gatccttcat ctggaactcg tgcaggtggc tatcggtcca tcccatcgcg
      721 tcctggattg caacatgaaa gtcccagata ctgtaggtct ccggcacctg aatcctcctc
      781 cagatgggag ggcggatctc ctgaagggtg atcttgaact ggtacacgtt ctcgaatgtt
      841 ttcttcttct ttggcatctc tctcacgctc ctctgcaatt ccgcaggcgc accggacgca
      901 ctccatcccc ggtcgtgcag agatctgttt ctccgaagtg atccttctcc ctaccgccaa
      961 aaaaaggttc ctgccgggat cttctctcct atccaccggg cgagagggag cacctgcagg
     1021 cccgggggcg ggtcggagtc caaccccgca aattccgtaa aaattccccc gctacacctc
     1081 atccccggga gttccggcgc atatgacccg caccgcccgg gcgaaactcc tctcctcagg
     1141 ccatcgcaac aactattgcc tccaggattt cacatctcat gagatgtctt cgtgtttcag
     1201 tgacccgcat atcctggagt tcttcgcaat aggattcgac ctgatggggg ggcatctccc
     1261 tccatctgcg gttgccccaa tcatcacgga tgccgaggat gtgctggaaa aatatcccga
     1321 caaccggggt gccgctcttg cgcttgatgc cctgaacgat ctcatagggg agcgggacga
     1381 ggcgctcatc gcccttgaga gtctggccat gctctctgag agctcgatca acatgctccg
     1441 cagggcgcgg cagctgatgc tctcggggaa gacggaggag gccgaaggcc tgcttttaga
     1501 gatcacccgg atgagaacag cgggatcggt accaagagaa ctccacatca tgctcgcgtt
     1561 cgcccgcctg ggcgataggg aagcgttcgc ccgcatatgg cacgacctga tcgagcggga
     1621 cggcctctta gaacccgtcc ctgctgaaga attcatcaaa tatcccggtg actacacgct
     1681 gcttggagag ttcccgttca gggaacagat cgagtctctt gaatacctct tttcgtacgg
     1741 ggttcaggag aacagagaag ccgaagtatt tgcttttatc cgtagtctcc cggattacct
     1801 ggagagcctc tccatccagg tagacctgga ggagcgggaa tacggtatct ccaggtgctt
     1861 cgcggcgttg ccggtgatca aagcaattgc tgaggggagc ctcgatgttg tcgagaagat
     1921 actctcgacg tacgatccga tatccgatga acggctcctt gaagagaccg gagagaacat
     1981 cgaacgtatt cgaaagtcgg gcgtggaggc cggcgtgttg aacgaactgt tgcgctggtc
     2041 cgttgatccc gtgcaaccgg ccgggaagtt actcgataca ttgcggaggc ataccggagg
     2101 ggacgatgag ccgatcctcg ctatgttcga tatggcgaac atgggggcat ccggggttgc
     2161 tgtcggggag ctggcctcat tactcctgga ggctcatcca gacaaccgct gccttataga
     2221 gagtgtatac ggttcgctga agtcggagga gcgatatgat gaggcgctgg cgtttgccga
     2281 ccgccacgaa ttcctgaaac aggatgacgc tcagttcgag agcttgaagc tggaagcact
     2341 tcgctcgtcg gatgaggagg cagcctttca gttcctcctc ggagggataa aggagaggcg
     2401 catgctggag cgctacgccg atctcttcga gctggcgctc gcgcttgacc ggatggatga
     2461 ggtcgcctcg ctcagatgga tctttgcgga agagaagggg gcaatcgcgg gatcgcatat
     2521 gctgaatgcg ctggaccgcc tgaagaagag ggatgtaaaa gagggcatgg cgctgtttga
     2581 gcggtcaaag agatccggtt ttccagaaga gcttgccctg atgctcctgg cgcgttctct
     2641 cctctcggcc ggctatccca aacgggtcgt cgggctccgt gaaaagatgc tcaagacgtc
     2701 gctgcctcca gaggagatct acccgctgct tatccgggca taccgggagc tcggcaggga
     2761 atccgaggca cgcgacgtgg aagcaatgct tgaggcggtg cgctgagcgg gaaccgggga
     2821 tagaaccgcg acggaaggtc tccccggatc attcgggggt gaggtgggta ttcaagggga
     2881 gatccttcgt cccggcggcg actcacccga tgccctgagc cgctctcccc tcaccgccgg
     2941 ccgaaccata ccacgaggcc gatgatcgct gcgagcggaa tcacccaccc ggccgccgcc
     3001 ttcggggtcg gggccggctc gcctcccgcc tgcgcctcga tccacgcgac cgcgtacgag
     3061 agctggacat cctccccggc catcgcccgc tcaagggtat ctcgatcgag cggcacacgg
     3121 accgtcgggg cgacaccgcc gatcatcgat ctgttgctgt cgacctggat ccgaccgagc
     3181 tcatcgagcg atgcaccctg cgggaaggcc gtcatgatac cgagcggcaa caccggtccc
     3241 atcgactcca tcccgaacgc cccgttcgtc ccgtaccacg agacgatcgc gccagccccg
     3301 gtctcggcaa agaccttcgg aagtccctcc cccgaactga tggtgtaggg gctgaccagg
     3361 agggcgaccg gcccttcgta gcggtccggc cggggctcgc tccaggactc ccagaggacc
     3421 gcctgctccc cggtcccggg gtcgtacgtg gtgccgtact cgtagaagac cggccggtcc
     3481 acgaaccacc cggcgaaagc ggcggccagg ttgtcgtccc caccccggtt gaaccggagg
     3541 tcgatgacga taccgggcgc accgccggcg atcgcagtct ccatcgccgc cctgaaaggc
     3601 tggtagacgt cgtaggactc ctcgtagacc gcgatatagg cgatccctcc cgggagggtc
     3661 cgggtggtca cggtctcgtt cgtgagcatc gccttgagtt ccgccgacga cccggccgcc
     3721 ccgtcgtttg tctcgcggcc gaggaagatg ctcgtccggg caagggtctc atagtcgtcg
     3781 tccgttgcgg tcaggctgac cgtgcgggga gtcgggccgg cgatgccgat ctccgtcgtc
     3841 gtcccgaccg gcgctcgggc gaggaaccgc tgcttctgga ggaggattcc ttccgccgtc
     3901 gagggtttga tcggcgtcca caggatcggc gtcgcgttga tcgcctcccc gatcggccga
     3961 ccgttccagg agaccacctc gtcgccgaac cggatgcccg cccgctcggc ctcgctcccg
     4021 ctcgcgacgt aggtcacgat cacctcgccc gaatcaagcc gtgcgacggc aaacccgtag
     4081 cccccgccga tatcggcatg tttcgccccg aagtcgtcag gggagaggac cagaacgtgc
     4141 ccgtcgggga tggcgaagat gaactcgcgg agcgtacggt agtacgcggc ggtatccttg
     4201 ctcctctcgg catccgcgat cttttgcgcg taggtcgcat acagcgcatc ccaatcgacg
     4261 ccgcgccact ccgtgaaggc gtagcgctcc tccatgcagg cgcaggcctc gcggaaagcg
     4321 tcgctccagg tcatgttgct gagatcgggc gcctctgtca ggttcgccat gatgggtccg
     4381 taggacgcca tcagctcttc ggtgtaggcc gtctcgttat tccgctcaaa cgggagggcg
     4441 gcatccgtcc agaccatccc gcacgagtcg gtatagaggc cggcggaggc gggcagcatt
     4501 acggagaaga gcaggagcag cacgatcaat ccggctctcg ctccgcgtga aatccttgaa
     4561 aacacgatgc tctaccttat gttactcttt cctgcagcag gatttggctt ttgtgatatc
     4621 ttccccgggc ctactacggt cagcgtcccg gagggttgcc ggagggcagg cacgatgtgg
     4681 ctatggatca ctccggcgga tatgcgatga gcccttcctt tacacccctg agaaatccgg
     4741 ccttctcgta gagccggaag acgttcggtt cgctccggcc ggtcaggagc atcaccttgt
     4801 agcagtttcg ctcccacgcg taacccggcg cgtgagtgag gagggtcgtc ccgaaccccc
     4861 ggttccggta ctcgcggtgc gtcacgacgt tctcgatgag cccgtagggg cgcgcgcccc
     4921 gcgtgaggtt tgagtggatg tggaggacgc acgtcgcgac cggcaccccc tcgtactcga
     4981 ggatgaagag atgggtccgc gggtcggcgg ggatctccag gcagatcctc tccagcactc
     5041 gatcgccggc tttctcgtcg gcatcgtgca ggtgcgcata gagggagagg accgcggcga
     5101 ggtcttcccg ggcggcctcg cggatgagca tcgtatcgct ctgcatgcac catcatccgc
     5161 tcctgcagag atcatctttg cgcttttacc ggcgagggag cctccgctgg atgtgcagga
     5221 attatctccg gcgaccaccc tgttggacaa cttatttacg gcactatgaa caaacatata
     5281 catcaaagcg cacgcatgcc gcgcggagcc catccatgcc gaactgcacc acgtttctcg
     5341 atgccaacgc cgctaacc
//
LOCUS       DAOSII010000119         5313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_13858_length_5313_cov_56.431343, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000119 DAOSII010000000
VERSION     DAOSII010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13858_length_5313_cov_56.431343"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PLV90_08410"
     CDS             complement(<1..282)
                     /locus_tag="PLV90_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOF97176.1"
                     /translation="MKIAIASGKGGTGKSTVAANLAYTIARSRDVVLVDCDVEEPNLH
                     LFFPAPAVDVPVTTPVPEIDPDRCTLCGECGRFCRFGALAVVKDCVLTFP"
     gene            complement(296..637)
                     /locus_tag="PLV90_08415"
     CDS             complement(296..637)
                     /locus_tag="PLV90_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOF97177.1"
                     /translation="MIVCITARAAGTDAPVEQRFGRAPYFVFVDTETGKSESVENPLV
                     DAAGGVGPRAAQVLSEHGATVLITGQVGGNAARALGTGGIKAYAYRGSGSVADALAAY
                     TAGNLQPIPLA"
     gene            complement(697..1053)
                     /locus_tag="PLV90_08420"
     CDS             complement(697..1053)
                     /locus_tag="PLV90_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOF97178.1"
                     /translation="MRIAIAQDGNQVSGHFGHCEGYAIFDVEGSIIYRRDDLENPGHE
                     PGRLPVFLAEHKIDLIIAGGMGPRAVDLFRANGIEVLLGISGNVDYVAQDYIAGRLSP
                     GESSCHHGAGEECGGH"
     gene            1214..3610
                     /locus_tag="PLV90_08425"
     CDS             1214..3610
                     /locus_tag="PLV90_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF97179.1"
                     /translation="MVQPLDIGEYRNAYEPEKVKCASTEEMRPLEEIIGQERALRALA
                     FGLEIREPGFNVYTAGAQGTGRMTAVRSFLDELAKAKPRAGDWVYVHNFANQYEPNAI
                     ALPAGRGPEFRDDMKRFIEDARRALPRAFESEEYAKRRDETLQSLQGNRADLIARINQ
                     RAQEAGFVIQMSPIGLLTIPIINGRPVPEEEFINLPDDVRTDIQRRRDALNTELRSTL
                     RQVQEIERQGAEAATRLNHDIALYAIGNLVAELKEKYADVAEIPEYIDAVQDDILENL
                     QAFLGMPEQPGAPPQFQAFIRELPFRKYEVNVVVDNTGTSGAPVVFEQNPTFQNLLGK
                     IEKEVQFGIFTTDFTMIRSGSLHAANGGYLVLTVEDLLRAPLSWDGLKTALKTGKAVI
                     EEPGERMGFITAKTIKPEPIPLDIKVALIGTPMIYQILYRMDPDFKELFKVKADFDIV
                     MDRDDENAGKYADFMCNLVREESLRHLDREAIARVIEYGSRLAADKQKLSTQFAAIAD
                     LIREANFYAAGGGADRIGKEHITKAIEEKIYRSNLIQKKIEEFIRRGIFLIDTEGEKV
                     GQVNGLSVMGLGDFMFGRPSKVTASIGVGREGIMDIEREAALGGPIHTKGVLIVSGYL
                     NNNYARDKPLALSARLVFEQSYEGIEGDSASSTELYALLSALSGLPLKQYLAVTGSVN
                     QKGEVQAIGGVNEKLEGFFEICKAKGLDGTQGALIPASNVQNLMLKEEIVEAARAGKF
                     RIYPVRTIDEGIEVLTGVPAGTRREDGTYEEGTVNYLVDRRLRDMAETMRGFQPTAAK
                     "
     gene            3616..4782
                     /gene="nifS"
                     /locus_tag="PLV90_08430"
     CDS             3616..4782
                     /gene="nifS"
                     /locus_tag="PLV90_08430"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOF97180.1"
                     /translation="MERRRSVYMDYAATTPVRPEVLEAMAPYFSERFGNPSSLYALAR
                     EAKEAVEEARGRAAAAIGADPGEVFFTAGGTEADNWAIKGTALRGKGDHIVTSAIEHH
                     AVIHPCRALEKQGYRVTYLPVDEFGRVDPADAEDAIADETILVSVMAANNEIGTIQPI
                     RAIADIAHDHGVPFHTDAVQAIGAYPVDVDDMGADLLALSAHKFGGPKGAGALYIRRG
                     TRVGTFMDGGAQERGRRAGTENVPGIVGLGRAIEVATADIEGHSRRLAAMRDRLIRGI
                     LDDIPDTRLNGHPVERLANNVSVAFRYVEGESILLLLDALGIAASTGSACTSASLEPS
                     HVLTACGLPHEEAHGSLRLTLGSRNTEEDVDYVLSVLPGVIGRLRDISPLRGEA"
     gene            4782..>5313
                     /locus_tag="PLV90_08435"
     CDS             4782..>5313
                     /locus_tag="PLV90_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOF97181.1"
                     /translation="MPGIFRAYDIRGRYPDELDEAMARRIGNAFARLLSAEQVVVGRD
                     MRLSAESLADAFAKGVLEAGADVADVGEVSTPLLNYAIIAGEFDGGAMVTASHLPGDM
                     NGVKLARRDAVPLSGDRGLPRLEAMVGEEPVARAPGSSRRVDMLDSYIGKVAGFVRNP
                     RPLTIVVDAGNGMAGPE"
BASE COUNT         1006 a         1684 c         1789 g          834 t
ORIGIN      
        1 cgggaaggtg aggacgcagt ccttcaccac ggcaagcgcc ccgaaccggc agaacctccc
       61 gcactccccg cagagggtgc agcggtcggg gtcgatctcc gggaccgggg tcgtcaccgg
      121 cacgtcgacg gccggggccg ggaagaagag gtggaggttc ggttcctcga cgtcgcagtc
      181 gacgagcacg acgtcacggg agcgggcgat ggtgtaggcg aggttcgccg ccaccgtact
      241 cttcccggtc ccgcctttgc cgctggcaat cgctatcttc atgccgaacc ctgtctcagg
      301 cgagggggat cggctgcagg ttcccggcgg tataggccgc aagggcgtcg gcaacgctcc
      361 cgctcccacg gtaggcatac gccttgatcc cgcccgttcc aagcgcacgg gcggcgtttc
      421 cgccgacctg accggtgatg aggaccgttg cgccgtgttc cgagaggacc tgggctgccc
      481 ggggcccgac cccgccggca gcgtcgacga ggggattctc aaccgattcg gacttccccg
      541 tctccgtatc gacgaagacg aagtagggtg cccggccgaa ccgctgttcg accggggcat
      601 ccgtgcccgc ggcgcgggcg gtgatgcaga ctatcatatg atgcactccg aaattattac
      661 tcatatgggc ataatcttat gggtcgatgc accccatcaa tgcccgccgc actcctcgcc
      721 ggcgccgtgg tggcaggagc tctcgcccgg cgagaggcgg ccggcgatat agtcctgcgc
      781 tacataatca acgttgccgc tgatgccgag gagcacctcg ataccgttcg cgcggaagag
      841 gtcgactgcc cgcggtccca ttccgccggc gatgatcagg tcgatcttgt gctccgcgag
      901 gaagaccggg agccgccccg gttcgtggcc ggggttctcg aggtcgtccc tccggtagat
      961 gatcgaaccc tcgacatcaa agatcgcata cccttcgcag tgcccgaagt gtccggacac
     1021 ctggtttccg tcctgtgcaa tcgcaattct catagggaca cctgtttttc gcggtctcgt
     1081 gcagggagaa ccgcacacaa tcatactaca cataagcgcg aattgggtta aaaccaccca
     1141 tcatgacatc cggggtatct tttttaaggt gggcaacacc ctgcggtgca acgagagaaa
     1201 cctggtgaga ctgatggttc aacctctgga tataggagag taccggaacg cttacgaacc
     1261 ggaaaaggtg aagtgcgcca gcaccgaaga gatgcggccg ctcgaagaga tcatcggcca
     1321 ggagcgagca ctgagggcgc tcgcgttcgg ccttgagata cgggagcccg ggttcaacgt
     1381 ctacaccgcg ggcgcccagg ggaccgggcg gatgaccgcc gtccggagtt tcctcgatga
     1441 gctcgcaaag gccaagcccc gggcgggcga ctgggtctac gtccacaact tcgcaaacca
     1501 gtatgaaccc aacgccatcg ccctcccggc ggggaggggg ccggagttcc gggacgacat
     1561 gaaacggttc atcgaggatg ccagaagagc gcttccgcgg gcgttcgaga gcgaggagta
     1621 tgccaagcgc cgggacgaga cccttcaatc gctccagggg aacagggccg acctcatcgc
     1681 ccggatcaac cagcgtgccc aggaggcggg gttcgtcatc cagatgagcc ccatcggcct
     1741 cctgaccata ccgatcatca acgggaggcc ggtccccgag gaggagttca tcaacctccc
     1801 cgacgacgtg cggacggata tccagcggcg gcgcgacgcc ctcaacaccg aactccggag
     1861 cacgctccgg caggtgcagg agatcgagcg gcagggggcc gaggcggcca cgcgcctgaa
     1921 ccacgacatc gccctctacg cgatcggaaa cctggtcgcc gaactcaaag agaagtacgc
     1981 cgacgtcgcg gagatccccg aatacatcga cgccgtccag gacgacatac tcgagaacct
     2041 ccaggccttc ctcggtatgc ccgagcagcc gggcgctccg ccccagttcc aggccttcat
     2101 ccgggagctc ccgttccgga agtacgaggt gaacgtcgtc gtcgacaaca ccgggaccag
     2161 cggcgcgccg gtggtcttcg agcagaaccc taccttccag aacctgctcg gcaagatcga
     2221 gaaggaggtc cagttcggca tattcacgac cgacttcacg atgatccggt cggggtcgct
     2281 gcatgcggcc aacggcggct acctggtcct caccgtcgaa gatctcctgc gggcgccctt
     2341 atcctgggac gggctcaaga ccgccttgaa gaccgggaaa gccgtcatcg aggagcccgg
     2401 agagcggatg ggattcatca cggcaaagac gatcaagccc gagcccatcc ctctcgacat
     2461 caaggtggcc ctgataggga caccgatgat ctaccagatc ctctaccgga tggaccccga
     2521 cttcaaggaa ctcttcaagg tcaaggccga cttcgacatc gtcatggacc gggacgacga
     2581 gaacgccgga aaatacgccg acttcatgtg caacctcgtc cgggaggaga gcctccggca
     2641 cctggaccgg gaggcgatcg cccgggtgat cgagtacggt tcgcggcttg ccgcggataa
     2701 gcagaaactc tcgacccagt ttgctgcgat cgcggacctc atccgagagg cgaacttcta
     2761 cgcggcggga ggcggtgccg accggatcgg gaaagaacac atcacgaagg cgatcgagga
     2821 gaagatctac cgctcgaacc tgatccagaa gaagatcgag gagttcatcc ggcgggggat
     2881 cttcctcatc gataccgagg gcgagaaggt cggccaggtg aacggcctct cggtcatggg
     2941 gcttggggac tttatgttcg gccggccgtc gaaggtgacc gcgagcatag gagtcgggcg
     3001 cgagggtatc atggatatcg agcgagaagc ggctctcggc gggccgatcc acacgaaagg
     3061 cgtccttatc gtcagcggtt acctcaacaa caactatgcc cgcgacaaac cgctcgccct
     3121 ctcggcccgt ctcgtcttcg agcagagtta cgaggggatc gagggcgact ccgcgtcgag
     3181 caccgagctc tacgccctcc tctcggcgct ctcgggcctc cccctgaaac agtacctcgc
     3241 cgtcacgggc tcggtgaacc agaaaggaga ggtccaggcg atcgggggcg tgaacgagaa
     3301 actggagggg ttcttcgaga tctgtaaggc aaagggcctc gacggcaccc agggagccct
     3361 gataccggca agcaacgtcc agaacctgat gttgaaggag gagatcgtcg aggcggcgcg
     3421 tgccgggaag ttccggatct acccggtgcg gacgatcgac gaggggatcg aggtcctcac
     3481 gggcgttccg gcaggcacgc gccgcgaaga cgggacgtat gaagaaggga cggtgaacta
     3541 cctcgtggac cggcgcctcc gtgatatggc ggagacgatg cgcgggttcc agccgacggc
     3601 ggcgaagtga acggtatgga gcggaggcga tcggtctaca tggactatgc agcaacgacg
     3661 ccggtgcggc cggaggtcct cgaggcgatg gccccgtact tctcggagcg gttcgggaac
     3721 ccttcctcgc tctatgccct cgcccgtgag gcgaaagagg cggtggagga ggcgcgggga
     3781 cgggcggcgg cagcgatcgg cgcagacccc ggggaggtct tcttcaccgc cggcgggacg
     3841 gaggccgaca actgggcgat caaggggacg gctctccggg ggaaggggga ccacatcgtc
     3901 acctcagcaa tcgagcacca cgcggtcatc cacccctgcc gggcgctcga gaagcagggt
     3961 taccgggtca cctacctgcc cgtcgacgag ttcggccggg tggaccccgc cgacgccgag
     4021 gacgcgatcg ccgatgagac gatcctcgtc tcggtgatgg ccgcaaacaa cgagatcggg
     4081 acgattcaac cgatccgcgc catcgcggat atcgcgcacg accacggcgt cccgttccat
     4141 accgacgctg tccaggcgat cggggcctat ccggtggatg tggacgacat gggagccgat
     4201 ctcctcgccc tctccgccca caagttcggc ggcccgaaag gggcgggggc gctctacatc
     4261 cggcgaggga cccgggtcgg gacgttcatg gacggcgggg cacaggaacg gggccgccgg
     4321 gccgggaccg agaacgtgcc cggcatcgtg gggctcgggc gcgcgatcga ggttgcgacc
     4381 gccgatatcg aggggcacag ccgccggctc gccgcgatgc gggaccggct gatacggggg
     4441 atcctcgacg atatcccgga tacccggttg aacggccacc cggtcgaacg gctcgcaaac
     4501 aacgtcagcg tcgcgttccg ctacgtcgag ggggagtcga tcctcctcct gctcgacgct
     4561 ctcggtattg ccgcctcaac cgggagcgcc tgcacatcgg cctcgcttga accctcccac
     4621 gtcctcacgg cctgcggcct cccgcacgag gaggcgcacg gttcgctccg gctcaccctc
     4681 ggttcgcgga acaccgagga ggatgtcgat tacgtcctct cggtcctgcc cggggtgatc
     4741 gggcggctcc gggatatatc gccgcttcgg ggggaggcgt gatgcccggc atattccggg
     4801 cctacgatat ccgggggcgc tacccggacg agctcgatga ggcgatggcg cggcggatcg
     4861 ggaacgcttt tgcccgtctc ctctccgcgg agcaggtcgt cgtcgggcgg gatatgcggc
     4921 tctcggcgga gtcgcttgcc gatgcgttcg caaaaggcgt gctggaggcc ggggcggatg
     4981 tcgccgacgt cggcgaggtg agcacgccgc tcctcaacta cgccatcatc gccggggagt
     5041 ttgacggcgg cgcgatggtg acggcctccc atctcccggg ggatatgaac ggtgtcaaac
     5101 ttgcgcgaag agacgcggtg cccctctccg gggaccgggg gctgccgcgc ctcgaggcga
     5161 tggtcgggga ggagccggtc gcccgggccc cggggtcttc tcgccgggtg gatatgcttg
     5221 actcctacat cgggaaggtg gccgggtttg tcaggaaccc ccggccgctc acgatcgtcg
     5281 tggacgcagg gaacggcatg gccgggccgg agg
//
LOCUS       DAOSII010000120         5219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_14171_length_5219_cov_49.528854, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000120 DAOSII010000000
VERSION     DAOSII010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14171_length_5219_cov_49.528854"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2375)
                     /locus_tag="PLV90_08440"
     CDS             complement(<1..2375)
                     /locus_tag="PLV90_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HOF97182.1"
                     /translation="MPEIYLKVDSAYPEDQGAGKARLDPDTMLQLRLSPGDLVAIEGK
                     RRTVAKVWRAMVNDWHQGKVRIDNFTRLNTGASIGDRIKIKTLDEEVEAKRVVLAPPE
                     DLPKQLPINYSSVVNKLIDFPVVKNDSVPIQAGLPFMQPQLVAFKAVVVEPENAVIIT
                     KNTKIEFSEKPAAGFEGVKRISYEDIGGLKGELQRVRETIELPMRHPEIFHKLGIEPP
                     KGVLLYGPPGTGKTLIAKAVASESGAHFISIAGPEVISKYYGESEQRLREVFEDARQH
                     APAIIFIDELDSIAPRREEVTGEVERRVVAQLLTMMDGLEERGQVVVIGATNRLDAID
                     PALRRPGRFDREIEIGVPPEGDRAQVLHIHTRGMPLGEGVDIAAIAQQTHGFVGADLA
                     ALAREAAIKALRRYLPEIDLEAEEIPAETLEKMEVQGKDFRDALRDVGPSAMREILLE
                     VPHTTWGDVGGLEEAKQDIREAVEYPLTRREQFEDLGIEPPKGVLLYGPPGTGKTLIA
                     KAVASESGANFVPVKGPQLLSKWVGESERAVREVFKKARQVAPSIIFFDELDALAPAR
                     GGGSESHVIESVLNQILTEIDGLEELRGVVVMGATNRPDMVDPALLRPGRFDRLVYIG
                     EPGRDDREKILRIHTRYMPIEGSTIEDVVDMTEGLSENAVEDLMLAVDAGRHTSIEDL
                     RSRRAMIEADGDEGLRRSLRRRKIVDLLSQQKIALDDPARTRLIRKIATETEGFVGSD
                     LEALGREAAMLAMREGASHVNATHFDRAQEKVHATMNERLRQYYGRVQQHF"
     gene            2422..3615
                     /gene="larC"
                     /locus_tag="PLV90_08445"
     CDS             2422..3615
                     /gene="larC"
                     /locus_tag="PLV90_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HOF97183.1"
                     /translation="MNVLLFDPFNGAAGDMIIGSLLDLGADEGVVRAAMRSVVGEPTI
                     ERVDRSGIRAVRVRAHAPVDHRTLEEVLDRVAESDTPAPAREMARRVFLRIHRAEESI
                     HGHGAHFHEVGADDAIADVVGACTALYSLAPDGVAVLPVALGRGFAVGAHGTFPIPAP
                     ATVAILAASDLRTVPGGEERELCTPTGAALLAEFSTTRPDDIGPAAIRAVGYGAGSRN
                     PPGRPNVLRSMIFATDEKAGGDRVDILETNVDDVTGETLAYTINRLMEEGARDASATP
                     IVMKKGRSGHLIRVISHPDATGRLMGVMAAELGTLGIRCIPMIHRAIAERTVEQITVT
                     ISGREFAIDVKCGWSGGEITSFKAEHEQAAACARDTGIPLREVTGTVEAAARRLFIER
                     GVLAS"
     gene            3612..4289
                     /gene="radB"
                     /locus_tag="PLV90_08450"
     CDS             3612..4289
                     /gene="radB"
                     /locus_tag="PLV90_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadB"
                     /protein_id="HOF97184.1"
                     /translation="MKPDRVSTGSRPLDDLLGGGLERRTITQIYGEPASGKSTFCLMA
                     AVASLRAGNSVIYIDTEGFSVERFAQVAGGHAAEFADRLYLFEPIDFAQQGAMIADTE
                     TLLRKKDGAPVDLLVMDSMTALYRTELDLGREAVRRLSHHVIKLLGLAKKYDIPVLIT
                     NQIYVDVERDRVSGLGGTALEHISKAIVRLEKRDSVRRAMLRKHRSRPEGISFDFVIT
                     EDGIRAV"
     gene            complement(4276..5094)
                     /gene="dapF"
                     /locus_tag="PLV90_08455"
     CDS             complement(4276..5094)
                     /gene="dapF"
                     /locus_tag="PLV90_08455"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOF97185.1"
                     /translation="MEIAFTKLQGNGNDFILIDEYQHEIIPEEMKGSFAALYCDRRFG
                     IGADGVLFLQASNHADVRMRLVQPDESEAAMCGNGIRCLAKYAYDAGYAGESCSVETG
                     AGVVPVAMGYVDGEFAATITMPEPAFERSAIPATGNGEYREDIRGFAVHAVNTGVPHA
                     VVFAEEVDAIDLAAVGPVIRNHPTFPEGANVNFVECAGNDILRVRTFERGVEAETECC
                     GTGATASAAVARRLGYVGETVRVETNGGPLVISFSKAVTMEGPAETVFFGVIPL"
BASE COUNT          875 a         1721 c         1675 g          948 t
ORIGIN      
        1 ttgaagtgct gctgcaccct cccgtagtac tgccgcagcc gttcgttcat cgtcgcgtgc
       61 accttctcct gcgcccggtc aaagtgcgtc gcgttcacgt gggatgcacc ctcccgcatc
      121 gcgagcatgg ccgcctcccg gccgagcgct tcgaggtccg agccgacgaa tccttcggtc
      181 tcggtcgcta tctttctgat gagccgggtc cgtgcgggat cgtcgagggc gatcttctgc
      241 tgggagagga gatcgacgat cttcctccgc cggagagagc gccggagccc ctcatcacca
      301 tctgcctcga tcattgcgcg gcggctccgg aggtcttcga tactcgtatg gcggccggcg
      361 tcgaccgcga gcatcagatc ctcgaccgca ttctccgaga gcccctcggt catatccacg
      421 acatcctcga tcgtagaacc ctcgatcggc atgtagcggg tatggatcct gaggatcttc
      481 tcacggtcgt cccgcccggg ctcgcctatg tagacgagcc ggtcaaaccg cccgggccgg
      541 aggagcgccg ggtcgaccat atccggccgg ttggtcgcac ccatcaccac gacgccgcgg
      601 agttcctcaa ggccgtcgat ctcggtgagg atctggttta gcacgctctc gatgacgtgc
      661 gactcgctgc cgccgccgcg ggcgggggca agggcatcga gctcatcgaa gaatatgatg
      721 gacggcgcca cctgccgggc cttcttgaag acctcgcgga cggcccgctc gctctcgccg
      781 acccacttcg agaggagctg agggcccttg acgggcacga agttcgcccc gctctcgctc
      841 gcgaccgcct ttgctatcag ggtcttaccg gtcccgggcg gcccgtagag gaggacaccc
      901 ttcggaggct cgatgccgag atcctcgaac tgctcccgcc gggtgagcgg gtactcgacc
      961 gcctcgcgga tatcctgctt tgcctcctca agcccgccga catcccccca ggtggtgtgc
     1021 ggcacctcga ggaggatctc ccgcatggcg ctcgggccca cgtcgcggag ggcgtcgcgg
     1081 aagtccttgc cctgcacctc catcttctcg agggtctctg ccggaatctc ctcggcctcg
     1141 aggtctatct cggggaggta acgccggagc gccttgatag ccgcttcacg ggcaagagcg
     1201 gcaaggtccg cccccacaaa cccgtgggtc tgctgggcga tggcggcgat gtccacgcct
     1261 tctccgagcg gcatgccccg ggtgtggatg tggagcacct gggctcggtc gccctccggc
     1321 gggacaccta tctcgatctc ccggtcgaac cggccggggc ggcggagagc cgggtcgatg
     1381 gcgtcgagcc ggttcgtggc cccgatcacc acgacctgcc cccgctcctc aagcccgtcc
     1441 atcatcgtca ggagctgggc aaccacacgc cgctcgacct ccccggtcac ctcttcccgc
     1501 cggggagcga tcgagtcgag ctcgtcgatg aatatgatgg ccggcgcatg ctgccgggca
     1561 tcctcgaaga cctcgcggag gcgctgctcg ctctcgccgt agtacttcga tatcacctcg
     1621 ggccccgcga tggagatgaa atgggcgccg ctctcgcttg cgaccgcctt tgctatcagg
     1681 gtcttacccg ttcccggcgg gccgtagagg aggacgccct tcgggggctc gatgccgagc
     1741 ttgtggaata tctccgggtg gcgcatggga agctcgatcg tctcgcggac acgctgcagc
     1801 tcgcccttca ggccgccgat gtcctcgtag ctgatcctct tgacgccctc aaacccggcg
     1861 gcgggtttct cggagaactc gatcttcgtg ttcttggtga tgatcacggc gttctccggc
     1921 tcgaccacca cggccttgaa cgcgacgagc tggggctgca tgaacgggag ccccgcctgg
     1981 atcggcacgg agtcgttctt cacgacggga aagtcgatca gcttattgac gacactgctg
     2041 tagttgatgg gaagttgttt cgggagatcc tcgggaggcg cgagcaccac tcgtttcgcc
     2101 tcgacctcct catccagcgt ctttatcttt atacgatccc cgatactggc accggtgttg
     2161 agccgggtaa aattatcgat tcggaccttt ccctggtgcc agtcgttcac catggcacgc
     2221 cagaccttgg ccacggtgcg gcgcttaccc tcgatagcga cgaggtctcc ggggctgagc
     2281 cgaagctgca gcatggtgtc agggtcaagc cgtgccttgc ctgccccctg atcctccggg
     2341 tacgcgctat ctactttcaa gtatatctcg ggcattacct ctaattctta tattcggggg
     2401 atttataagt actggaggcg catgaacgta cttctcttcg atccgttcaa cggggccgca
     2461 ggcgacatga tcatcggctc gcttctcgat ctgggggcgg acgagggagt tgtccgggca
     2521 gccatgcgct ctgtcgtcgg cgaaccgaca atagagcggg tagaccgttc cgggatccgc
     2581 gcagtccggg tgagagcgca tgcaccggtc gaccaccgta cgcttgagga ggtgctcgac
     2641 cgggtggccg agagcgacac ccccgcccct gcacgggaga tggcccggcg ggtctttctc
     2701 cggatacacc gggctgaaga gagcatccac ggacacgggg cacacttcca cgaggtgggt
     2761 gcggacgacg ccatagccga tgttgtgggg gcctgcaccg ccctgtactc cctcgccccc
     2821 gacggtgtcg ccgtcctccc ggtcgccctc ggccgtgggt ttgccgtcgg ggcccacggc
     2881 accttcccta tcccggcccc cgcgaccgtc gctatccttg ccgcatcgga cctccggacc
     2941 gttcccggcg gcgaggagcg agaactctgc accccgacgg gggccgctct ccttgccgag
     3001 ttctcgacga cccggcccga cgatatcggg ccggcggcga tcagggcggt cggctacggg
     3061 gccgggagca ggaacccgcc gggccggccg aacgtcctcc gctcgatgat cttcgcaacg
     3121 gatgagaagg ccggcggcga ccgggtcgat atcctcgaga cgaacgtcga cgacgtcacc
     3181 ggggagaccc ttgcctacac gataaaccgg ctgatggaag aaggagcacg ggacgcaagc
     3241 gccactccca tcgttatgaa gaaggggcgg agcggccatc tcatccgggt catctcccac
     3301 cccgatgcga ccggccgcct catgggggtg atggccgcag aactcgggac gctcggcata
     3361 cgctgcatac cgatgatcca ccgggcgatc gccgaacgga cggtcgaaca gatcacggtg
     3421 accatcagcg gccgggagtt cgctatcgac gtcaaatgcg ggtggtcggg aggggagatc
     3481 acctcgttca aagcggagca cgagcaggcg gcggcgtgtg cccgggacac gggcatcccc
     3541 ctccgcgagg ttaccggcac cgtcgaggcg gcggcacgtc gcctcttcat cgagcggggc
     3601 gtgctcgcgt catgaagccg gaccgggtca gcacgggaag ccggcccctc gacgacctgc
     3661 tgggcggggg actcgagcgg cggacgatca cccagatcta cggcgaaccc gcgagcggca
     3721 agagcacctt ctgcctgatg gcggcggttg cgagcctccg ggcggggaac tcggtcatct
     3781 atatcgatac ggaaggattc tcggtggagc ggttcgccca ggtcgccggg gggcatgccg
     3841 cggagttcgc cgatcggctc tacctctttg agccgatcga tttcgcccag cagggggcga
     3901 tgatcgccga tacggagacg ctgctcagga agaaagacgg cgcacccgtc gacctcctgg
     3961 tgatggattc gatgaccgcc ctctaccgga cggagctcga cctcgggcgg gaggcggtgc
     4021 ggagactctc gcaccacgtg ataaaactcc tcgggcttgc aaagaaatac gacatccccg
     4081 tcctgatcac gaaccagatc tacgtggacg tggagcggga ccgtgtctcc ggcctcgggg
     4141 ggacggcgct tgagcatata tccaaggcca tcgtgcgact cgaaaagagg gatagcgtcc
     4201 ggcgggccat gctccggaag caccggtccc ggccggaagg gatttcgttt gatttcgtga
     4261 tcaccgaaga cgggatcaga gcggtataac accgaagaag accgtctcgg cggggccttc
     4321 catcgtgacg gctttgctga agctgatgac gagcgggccg ccgttcgtct cgaccctcac
     4381 cgtctccccg acgtagccga ggcggcgggc gaccgccgcg gaggccgtgg cgccggtgcc
     4441 gcagcactct gtctcggcct cgacgccgcg ctcgaatgtc cgaacccgga ggatatcgtt
     4501 tccggcgcac tcgacgaagt tgacgtttgc gccttcggga aaggtgggat ggttccggat
     4561 gaccgggccg acggcagcga gatcgatggc atcgacctct tctgcaaaga cgacggcgtg
     4621 gggaacgccg gtgttcacgg cgtggacggc aaacccgcgg atatcctccc ggtactcccc
     4681 attcccggta gccggtatgg cgcttcgctc gaacgcaggc tccggcatcg tgatcgtcgc
     4741 ggcgaactcg ccgtccacat agcccatcgc caccgggacg actccggccc cggtctcgac
     4801 cgagcaggac tctccggcgt agccggcgtc ataagcgtac ttcgcgaggc accggatgcc
     4861 gttcccgcac atggcggcct cgctttcgtc gggctgcacg agccgcatcc ggacatcggc
     4921 gtgattggac gcctggagaa agaggacgcc gtcggcaccg ataccgaacc tgcgatcgca
     4981 gtaaagcgcc gcaaacgatc ccttcatctc ttcgggaatg atctcgtgct ggtactcgtc
     5041 gatcaggata aagtcattgc cgtttccctg cagtttggtg aacgcgatct ccatggatag
     5101 tagcctctct atgataggta ttgggagcgt cgagagataa accgtggtgc cttcaccgtc
     5161 gttcctccac ccttgccggg caagcccgaa gagtaactat atacccggcg gcccgcact
//
LOCUS       DAOSII010000121         5162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_14392_length_5162_cov_48.971216, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000121 DAOSII010000000
VERSION     DAOSII010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14392_length_5162_cov_48.971216"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(580..1845)
                     /locus_tag="PLV90_08460"
     CDS             complement(580..1845)
                     /locus_tag="PLV90_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOF97186.1"
                     /translation="MGRSDMQGAVQETAGSDRAPESIILRVALLSLAVNIGLVVAKLA
                     LSVYSGSLALRADGIHSFVDVVASLALIAGVLLSTRRRRDFPYGLYKVENVVAVIIAL
                     LIFFTAFEIAAEAMTGETSAHPYGGWVLFIVAAFIPVPYILGTYEVNVGRKYNSPSMI
                     ADGKQHQMDVLSSSVVFFALLGQFFGIPLDRVAAVIVAVFIVRAGWDILKDSMKTLLD
                     ASVDYDTLSEIRTIIRSDPVVSRIRSVTGRNSGRYIFVEAVVEMATSDLKEAHRASER
                     IEEAIHLRVRNIERVVIHYEPEEKVHLRYAVPLQDREGLIGDHFGEVPYFAIFDVDVR
                     ENRIERREFIANPFADVEERKGVQVAEMLLAQKVDVVLLHHSLSGKGAGYAFEAAGVE
                     MRMTAAVTLAEAIADVAGHHGVEGGAPPR"
     gene            complement(1970..2578)
                     /locus_tag="PLV90_08465"
     CDS             complement(1970..2578)
                     /locus_tag="PLV90_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOF97187.1"
                     /translation="MIDLSIFLAEHPYIDHSSPRIRAKAGELFSGIESSLERTRIAYE
                     FVRDEIPHSFDIGSDAITARASDVLARGTGICHAKANLLAALLRGFGIPAGFCYQHIT
                     LADDDSLGYCIHCYNAVHVEGRWIFLDARGNAGGRQALFSPGKPILAYPNRSEYDEYF
                     WKGIYASPQMGVMRMLDAAVTRQDVIDNLQDYIEGEPDIPGW"
     gene            2795..3385
                     /locus_tag="PLV90_08470"
     CDS             2795..3385
                     /locus_tag="PLV90_08470"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOF97188.1"
                     /translation="MDIFDEIYRGTPPWDIGRPQKAFIELAGRGEIRGSVLDIGCGTG
                     EHALFVAGAGHEALGIDTAPLAIQKAREKAAERGLPARFLVGNVLNLSGLNGRFDTVI
                     DSGLFHTLSDKDRPVLAESLATVLAPGGRYFMLCFSDRNPGEYPLPRRIAMAEIRDTF
                     QDGWSIDYVRPAVFENTVQADGHLAWLASISRKAQE"
     gene            complement(3592..4899)
                     /locus_tag="PLV90_08475"
     CDS             complement(3592..4899)
                     /locus_tag="PLV90_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOF97189.1"
                     /translation="MTACAQEYETKRTNPADAIRLVKSGNTVVYGMSISQPPALLTAL
                     ADAARAGSLKDVRIYTFLPREHAARSVLAPDLCDCLENYSWFVGAVDRDLTKVGLNYH
                     VPSYLHQIPTLVAEFMEADVVMTTVSPMDTAGYFSFGTGNDYISTAARHGKRFVVEVN
                     RNMPRVFGDSQVHISEVDAIVENHVPLLEIAAPDPKPEDDIIGPAIAEMIPDGATIQL
                     GIGNLPNAVARYLTGHTDIGVHTELFTEGMVDLIEQGVITGRRKTLHPRKHVFTTAAG
                     TRRMYEFMDDNPAMESYPASYVCDPGIIAKNDTMISINSVLEVDLLGQANAEYLGGTT
                     FSGTGGQLDFVRGAFASRGGKSILAFYSTAKGGTISRIVPRLPLGSIVTTPRADTDFL
                     ATEHGIVSLKGRSTKERALSIISLAHPKFRDWLMEEAENMYLL"
BASE COUNT          972 a         1598 c         1616 g          976 t
ORIGIN      
        1 gaactgcatg ccgagggctt ttgcaaggac gacgcccacg gtgctcttcc ccgcaccggg
       61 catgccgatg aggacgatgt tttcgtggtg atgcatgctg ctcccgggac cctatggaga
      121 ggaagcccca ttcctggtac tccggtggga tttcggcggg gatgaaggtt ttgttcggaa
      181 aaaacgctcc tcccccgggc catgcccgta tcatggggcg ggccgcgggg tgttcggagc
      241 gatcggggtc tcagggcgat ccggcgcggt tcacactcac gaaaagagcg acaaccccta
      301 taatgagagc gataactccg gataccgccc atagcggcga tgggccgaca acctcctcac
      361 agtttgctat gcagagtatg gggcgcatcc ggaggattcc tgtaccctgc aggagccata
      421 acaggccgag aaaccccacg aatgcgccga aaaatgttcc ggcgatcggt tttgcattca
      481 tgtgcgatcg aacgccaccc tcgacgccgg ctgcgataat tcctccgctt caaaggatca
      541 tgatcccgtt cggcaccggc cggaccatgc agcaagggat caccggggcg gggcaccacc
      601 ctccaccccg tgatgcccgg ccacatcggc gatcgcctcc gcaagcgtca ccgcggcggt
      661 catccgcatc tcgacccccg ccgcctcgaa cgcgtagccg gcccccttcc ccgagagcga
      721 atggtgcagg agaaccacgt cgaccttctg cgcaagcagc atctccgcta cctgcacgcc
      781 ctttcgctcc tccacatccg cgaaggggtt ggcgatgaac tcccgccgct cgatgcgatt
      841 ctccctgaca tcgacgtcaa agatcgcgaa gtacgggact tcaccgaagt ggtcgccgat
      901 caggccctcc cggtcctgca gcggcaccgc ataccgcagg tgcaccttct cctcgggttc
      961 gtagtggatg actacccgct cgatgttccg aacccggagg tgtatggcct cctcgatacg
     1021 ctcgctcgcc cggtgcgcct ccttgaggtc cgatgtcgcc atctcgacca ccgcctccac
     1081 gaagatgtag cgcccggagt tgcggcccgt gacgctcctg atacggctca ccaccggatc
     1141 ggaccggatg atggtgcgga tctcgctgag cgtatcgtaa tcgaccgagg catcgaggag
     1201 ggtcttcatg ctgtctttta agatatccca gcccgctctg acgatgaaga cggcgacgat
     1261 gacggccgcg acccggtcca ggggtatgcc gaagaactgg ccgaggagcg cgaagaagac
     1321 gaccgaggag gagaggacat ccatctggtg ctgtttcccg tcggcgatca tgctcgggga
     1381 gttgtacttc cgccccacgt tcacctcgta tgtaccgagg atgtagggca ccgggatgaa
     1441 ggcggcgacg atgaagagga cccatccgcc gtaggggtga gcggaggtct cgcccgtcat
     1501 cgcctccgcc gcgatctcga aggcggtgaa gaagatgagg agggcaatga tcaccgcgac
     1561 gacgttctca actttgtaga gcccgtaggg gaaatcccgg cgcctgcgtg tggagagaag
     1621 cacgcctgcg atgagcgcaa gcgaggcgac gacgtccacg aacgagtgga tgccgtccgc
     1681 acggagggcc aggcttccgg aatagacgga gagtgccagt ttcgcgacga ccagcccgat
     1741 gttgacggcg agggagagga gggcgacacg caggatgatg gattccggcg cccgatccga
     1801 tcccgctgtc tcctgcaccg ctccctgcat atcggatcgc cccataatgc attccttggc
     1861 gtggcgcatc catcaatcta accattgtac ccaatccgcc ctgacaggcg ccagagcagc
     1921 ggctccgccg gcgcgaacgg ccgtgttacc cattccgccg catctcgtat caccaaccgg
     1981 ggatatcggg ctcgccctcg atatagtcct gcaggttatc gatgacgtcc tgccgggtca
     2041 cggcggcgtc gagcatccgc atgacgccca tctgcggcga tgcatagatc cccttccaga
     2101 agtattcgtc gtactcactc cggttgggat acgcgaggat gggttttccc ggcgagaaga
     2161 gcgcctgccg gccgccggcg ttcccgcgtg cgtcaagaaa gatccagcgg ccttccacgt
     2221 ggactgcgtt gtagcagtgg atgcagtagc cgagcgagtc gtcgtccgca agggtgatat
     2281 gctggtagca gaacccggcg gggattccga atccgcggag cagggccgcg agcaggtttg
     2341 ccttcgcgtg gcagatccct gttccccggg caagcacgtc ggaggccctc gccgttatgg
     2401 cgtccgagcc gatatcgaac gagtgcggga tctcgtcgcg aacgaactcg tatgcgatcc
     2461 gcgtccgttc gagagagctc tctatcccgg agaacaactc tccggccttc gcccggatac
     2521 gcggcgacga gtgatcgata taggggtgct ctgcaaggaa gatgctcagg tcgatcatgc
     2581 gaggagggtg ttgccgggtg gttgtactgc gttggcgccg gggttattta agtatccggg
     2641 cggttgtgct gtccggcatg ctcggtggga tgcgggtgcg gaccgggatg ctgcagtgct
     2701 cccctcacct cagaccgtat gacccccgcc tctcagtcca acctgccttg agcagcacaa
     2761 aacctgttaa gccgcacacg gcccatactg cggtatggac atcttcgacg agatctaccg
     2821 gggcacgccg ccctgggaca tcggccggcc ccagaaggcg ttcatcgaac tggccgggag
     2881 gggggagatc aggggttccg tgctggatat agggtgcggg acgggagagc acgccctctt
     2941 tgttgcgggc gccgggcacg aggcgctcgg gatcgacacc gccccccttg cgatccagaa
     3001 ggcccgggag aaggccgctg aaagagggct cccggcgcgc ttcctcgtcg gtaacgtcct
     3061 gaacctctcc ggcctcaacg ggagattcga taccgtcatc gattcggggc tcttccatac
     3121 tctgtccgac aaagaccggc cggtcctcgc ggagagcctc gcaaccgttc tcgcgcccgg
     3181 cgggagatac ttcatgctct gcttcagcga ccgaaacccc ggcgaatacc ccctgccgag
     3241 gaggattgcg atggcagaga tccgggatac cttccaggat gggtggtcca tcgactatgt
     3301 caggccggcg gtcttcgaga acaccgtcca ggccgacggg cacctggcct ggcttgcgtc
     3361 gatatcgaga aaggcgcagg aatgacgtga tcggccgtga cggcacgacg cagacgctga
     3421 tcgagcgggg cggagggtgc tctaccgatt ttctctcatc gctcccggtg agatggaact
     3481 cgtctacgcc gagggaagcg atctcttcaa catcgcagtc ggctcgctga aaaatatctg
     3541 agagtctttt cctctttcgt ttgaagcaac cgcccgggcg ggagaccccg ttcagagcag
     3601 atacatattc tccgcttcct ccatcaacca gtcccggaac tttgggtggg cgagagagat
     3661 gatcgagagc gcccgttcct tggtagacct gcctttcagg ctgacgatcc cgtgttcggt
     3721 agcaaggaaa tcggtatcgg cccgcggggt ggtgacaatg gagccgagcg ggaggcgggg
     3781 aacgatgcgg gagatggttc cgcctttcgc cgtggagtag aacgcgagga tggacttgcc
     3841 tccccgcgag gcgaacgccc cccgcacgaa atccagctgg ccgccggtgc cggagaacgt
     3901 cgtgccgccg aggtactccg cattcgcttg ccccagcagg tccacctcga gcaccgagtt
     3961 gatcgagatc atggtatcgt tttttgcaat gattccggga tcgcagacgt aggaggcggg
     4021 gtaactctcc atcgccggat tgtcatccat gaactcgtac atacgccgtg ttccggccgc
     4081 cgtggtgaag acgtgcttcc tcggatggag cgtctttctc ctcccggtga tcaccccctg
     4141 ctcgatgagg tcgaccatgc cctcggtgaa gagctcggta tggacgccga tgtccgtatg
     4201 cccggtcagg tatcgggcaa ccgcattcgg caggttcccg atcccgagct ggatcgtcgc
     4261 gccgtccggg atcatctcag caatcgccgg cccgatgata tcgtcctccg gttttgggtc
     4321 gggagcggcg atctcgagga gcgggacgtg gttctcgacg atcgcgtcga cctccgagat
     4381 atgcacctgt gaatcgccga agacccgggg catattccgg ttcacctcga cgacgaacct
     4441 cttaccgtgg cgggctgcag tcgagatata gtcatttccc gtcccgaaac tgaagtagcc
     4501 ggccgtatcc atcggcgaga cggtcgtcat cacgacgtcg gcctccatga attcggcaac
     4561 gagcgtgggg atctggtgaa ggtaggaggg gacgtggtag ttcagcccga ccttcgtcag
     4621 gtcccggtcc acggcaccta cgaaccacga gtagttctcg aggcagtcgc agagatccgg
     4681 tgcaaggacc gaacgggcgg catgttcccg ggggagaaac gtgtagatcc tgacatcctt
     4741 cagcgaccct gcccgggcgg catcggcaag agccgtgagc aacgccggcg gttgggagat
     4801 gctcatcccg tagaccacgg tatttccact cttcaccagc cggattgcat ccgcggggtt
     4861 ggttctcttt gtctcgtact cctgagcaca ggcggtcatg tagtacctcg taaccggtac
     4921 gtccggtcat aacgtactat ggaggcgccc ctgtattaat cctggccggc aaatccattt
     4981 tctgccaaaa tatcgcctgt atcggtaaaa tgacgcagta catcgcgttt tctgagagtc
     5041 ggtcccccct cgcccggtct tgaaactcac gaccccgtag caccgatcaa gcgggagggg
     5101 cgggatcgcc tgtgtgtacg tccgcgccgt gccgaagacc ggggtcatgc cgtgccgccc
     5161 gc
//
LOCUS       DAOSII010000122         5046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_14825_length_5046_cov_43.391104, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000122 DAOSII010000000
VERSION     DAOSII010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14825_length_5046_cov_43.391104"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            593..2125
                     /locus_tag="PLV90_08480"
     CDS             593..2125
                     /locus_tag="PLV90_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOF97190.1"
                     /translation="MTTRLPERPGTLPGPCQPIALLIGAVICLLLAQSVAAAPLFVAA
                     SDSSPADRAQADYTCDGVDDQVEIRAALAALPGGGTVALANGTFNCSGSLIPRANTTL
                     RGEGPDATFILFRENGRLNVSEEHITLDGFQIAGADYKNMSTDTTLDLGRWLGVLTIY
                     ASHTKVRNVTGTADASIQAVFLLLHNPNVYASVLEDVEFTNCRAVDTGTYGFLHNAWG
                     SENTTIRNIRYENCSAINCGRYGAFNAWVTGFDFAEQNNIEGLRVSNCSARGSLESGF
                     HFEWDAEKRDCVFINCVSTENGQKPWPETYLVGDPHYFGAGFYLPGGRVSLVNCHAGD
                     NRAFGYFIGNPDGMLLYNCTENGTGRSPIFGWSARPIAYCILQSLPVTKNPSVVMDHC
                     SSLDTNGWGLFICGASNVLIRNFTLTDPAGIRGVGAMLGCPGDELPVNSTIGVGISHS
                     AIDLAASGSRPHTLIYIVNNRNVTYSGRIASGAAHPVLFEGSAGDVDLSALKVLPASG
                     TV"
     gene            2480..4129
                     /locus_tag="PLV90_08485"
     CDS             2480..4129
                     /locus_tag="PLV90_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97191.1"
                     /translation="MSAKPYRGARNRRIRIAIALLIALVGIAVLVLHLYPPVPAPPPP
                     PPPPFTPLPTTPVVPPEVPGSTITVAASDSSPASKARADIQCDGIDDQRDIQAAFDAL
                     PSGGTVALTEGRFNCAGSIYPHANTMLRGEGPDKTFVEFSRNGRLIVSRESVTLYNFH
                     VEGTNYPAGDDRWLGVVTIYASHARILAVEGTADATTQAVFLLLHDPGVYVPVLQDIE
                     FTNCRAADTGTYGFLHNAWGTENRVIRDIRYENCAAINCGRYGAFNPWVTGFDFAELN
                     DIEGLRVKNCLAEGNLESGFHFEWDPEKRDCILENCVSRNNGQKSYPTKPYQEDDTST
                     HYFGCGYYAPRGDITFVSCYSEENSQYGFYATNGGKLYACVDRGVGAGKTDYRIIQPA
                     SFYAAPTRSIAPSLILENCSSIDSNGYGLHIDFASDVRIVNFHLENPAGIDGKATNLG
                     GSQGGPLTNSVVNIYASGDRAETLIWARNNENVEYSGRIISNTAKPFVIEGDRTRKVR
                     VRDMEIVSANLAPFTNGVVLASTVPAGAVTFENVAVIPAVR"
     gene            4671..>5046
                     /locus_tag="PLV90_08490"
     CDS             4671..>5046
                     /locus_tag="PLV90_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOF97192.1"
                     /translation="MFLIVGLVPIVGALETTPTTIVAASDSSAAAKAAAAYVCDGVND
                     HVEIQAALNALPSSGGVVLLSSGTYNCAGIIAPKAGSTLKGEGETKTNLVFTHDGRIN
                     VDREYVTLDGFGVKGSGYSSGVK"
BASE COUNT          989 a         1548 c         1516 g          993 t
ORIGIN      
        1 ccgggcaagc atcccgtcga ggaacgtgat aaggtcgtct acatcctcat gggtctctac
       61 atcgccctcg acctcgaggc ggaggttttc gtagttctcg aggttgacgg tgatcccgat
      121 ggtgacttcc ttctttccgg gcatatgctt ataattattg ttttccatat atacaattcc
      181 accggccgcc ggaattgccc tgaaccgccc cttcttcccg tcatccgcac ccggcagcga
      241 cccgcaccgt ggatggacga gccggagaaa tacaagcaac tttcctgaaa tcggggcctg
      301 atcctgtcct gccagccctg cgcgcgggct ctcccgccgt gagtatctca aacccggatc
      361 ccggagtaac gcatcgattt cctcacccat caccttttat actgccgcgg ctatacctgc
      421 cgtgcccgat cgcgccgggc cgcccggtta tggtaaggcg gtccgcctgt cgggtccggc
      481 aaccggatct ccggtagatc cggtatgaca gggcctgctc gctccatagc agggcgctaa
      541 agcctctggt acagcatacc agggatcctg cacgatacga ccacggagtg tcatgacgac
      601 ccgcctgcct gaaagaccgg gaacccttcc cggcccgtgc cagccgatcg ccctccttat
      661 cggcgcggtg atctgccttc tgctcgccca gagcgttgct gcggcgccgc tctttgtggc
      721 ggcgagcgac agcagcccgg cagacagggc acaggcggat tacacctgcg acggggtaga
      781 cgaccaggtg gagatccggg cagcacttgc tgctctgccg ggcggaggca cggttgccct
      841 cgcaaatggg acattcaact gttccgggag cctcataccc cgggcgaaca cgacgcttcg
      901 cggggagggg ccggatgcga cgttcattct gttccgcgag aacgggaggc tcaatgtctc
      961 ggaagagcat atcaccctgg atgggtttca gatcgcaggg gccgactaca agaatatgag
     1021 cacggatacg acgctcgacc tcggccggtg gctcggcgtc ctcaccatct atgcgagcca
     1081 tacaaaggtt cgaaacgtca cgggcactgc cgatgcgagc atccaggcgg tcttcctcct
     1141 gctccacaac cccaacgtct acgcatccgt ccttgaagac gtcgagttca cgaactgccg
     1201 tgccgtggac accgggacct acgggttcct ccacaacgcc tggggatcgg agaacacaac
     1261 gatccggaac atccgctacg agaactgctc cgccatcaac tgtggcaggt acggggcgtt
     1321 caacgcctgg gtgaccgggt ttgactttgc cgagcagaac aacatcgagg gcctcagggt
     1381 gagcaactgc tccgccaggg ggagccttga gtccgggttt cacttcgagt gggatgcgga
     1441 gaagcgggat tgtgtcttca tcaactgcgt gagcacggag aacgggcaga aaccctggcc
     1501 cgagacctac ctggtgggcg acccgcacta cttcggggcc ggcttctacc tccccggcgg
     1561 acgggtatcc ctggtgaact gccatgcagg ggataacagg gcgtttggtt actttatagg
     1621 caatccggac gggatgctcc tctacaactg caccgagaac ggcaccggac gctcccctat
     1681 cttcggctgg tctgcgcgac ctatcgccta ctgcatactc cagtcgctgc cggtcacgaa
     1741 gaatccatcc gtcgtgatgg accactgcag cagcctcgac acgaacggat ggggcctttt
     1801 tatctgcggc gcatcgaacg tgctcatcag aaacttcacc ctgaccgatc cggctggcat
     1861 caggggggtc ggggcgatgc tgggatgtcc cggggatgaa ctcccggtga actcgacgat
     1921 cggggtgggc atctctcact cggccatcga tcttgccgca tcgggaagcc ggccccatac
     1981 gctgatatac atcgtgaaca acagaaacgt cacgtactcc ggcaggattg cctccggcgc
     2041 cgctcatccg gtcctgttcg aggggtcggc gggggatgtg gacctgtcgg ccctcaaggt
     2101 attgccggcc agcggtacgg tgtagcggcg gcatcgccgg ggccgggggt atctcgtctc
     2161 ttgaagagat ctcgcatacg ctgcccttcc cccctgcccg gcaaggatac cccgacgtta
     2221 gccctgcgga accctgggcc cgaccctccg ggggaacggg gcgactaaac tcttaaatgg
     2281 tacctccgcg ctatacgggg tgttgaccgg gcaggaggca gacgggtcat ccgtacccgc
     2341 gcccggggag tttccgctgg cagggccgcc ttcggatggt ccgatccagc ctgagcaggg
     2401 agtgcatcgt caggtcgcca ggggcgatgc cgcaggagat ccgacagcgt gagaagagta
     2461 acgggcaggt tgtagtctca tgtcagcgaa gccgtatcgg ggggccagga acaggcgcat
     2521 acgtatcgcc atcgcactcc tgattgctct ggtcggtatc gccgtactgg tcctgcacct
     2581 ttatcctccg gtgccggcgc ccccgcctcc tcctcccccg ccgttcaccc cgctgcccac
     2641 gactccggtc gtgcctccgg aggtgcccgg atcgacgatc accgtcgcgg cgagcgacag
     2701 cagccctgca tcgaaagccc gggccgacat ccagtgcgac ggcatcgatg accagcgcga
     2761 catccaggct gcgttcgacg ctctgccgtc gggaggcacg gtcgccctga ctgagggccg
     2821 gttcaactgt gccgggagca tctaccccca tgcaaacacc atgctccgcg gggaggggcc
     2881 agataagacg ttcgttgagt tcagccggaa cggtcggctc atcgtctccc gtgaatccgt
     2941 cacgctctac aactttcatg tcgagggcac caactatccc gccggagacg atcgctggct
     3001 cggtgtggtc accatctacg cgagccacgc gaggatcctt gccgtcgagg ggactgccga
     3061 tgcgaccacc caggcggtct tcctcctcct ccacgacccg ggtgtctacg tgccagtgct
     3121 ccaggatatc gagttcacga actgccgtgc cgcggacacc gggacctacg ggtttctcca
     3181 caacgcctgg ggaacggaga acagggtgat cagagatatc cgttacgaga attgcgccgc
     3241 aatcaactgc ggcaggtatg gggcgttcaa cccctgggtg accgggtttg acttcgcgga
     3301 gttaaacgac atcgaggggc tccgcgtgaa gaactgtctc gcggagggga acctggagtc
     3361 cggctttcac ttcgagtggg accccgagaa gcgggactgc atacttgaga actgcgtgag
     3421 cagaaacaac ggacagaaat cctacccgac gaagccctac caggaggacg atacgtccac
     3481 gcactacttc gggtgcggct actacgctcc ccggggggat atcaccttcg tcagctgcta
     3541 ctccgaggag aacagccagt acgggttcta tgcgacgaac ggcggcaagc tctacgcctg
     3601 cgtcgaccgg ggcgtcggcg ccgggaagac cgattaccgt atcatccagc cggcatcgtt
     3661 ctacgccgcc cccacccgat cgatagcgcc gtcgttgata ctggagaact gctcaagcat
     3721 cgactcaaac ggctacggcc tccatatcga ctttgcaagc gacgtccgga ttgtgaactt
     3781 tcacctggag aacccggccg ggatcgatgg aaaggcgacg aaccttggcg ggtcgcaggg
     3841 agggccgctc acgaactcgg tggtgaatat ctatgcgtcg ggtgaccggg ccgagacgct
     3901 gatctgggcc cggaataacg aaaacgtcga gtattctggc aggatcatct cgaacaccgc
     3961 gaaaccgttc gtgattgaag gagatcgcac cagaaaggtc cgggtcaggg atatggagat
     4021 cgtctccgcg aacctcgcgc ctttcaccaa cggtgtggtc ctcgccagta cggtgcctgc
     4081 cggagcggtg acgttcgaga acgtggctgt tattccggcg gtgcggtgac gcaccgatcg
     4141 ccattccggg cctggccccg ggagccccga tgcattgcac gggttctacc ggcgaccggg
     4201 tagtcgagat tgtttgccgg ccgatcccgc cctgtcccgg gcattgcatg gggggtgccg
     4261 cgggtattcc cctccggtcc tccggggtgc ggccgggggc ggcagatcgc ccccgcctgc
     4321 cggacccggg gatctatgac ttttccgggc ttttcatgaa tttttcagta atccggggct
     4381 tatttgcctg ccagaactac gcccctcgtt cccgggacag aacgggtttt atcccgggtg
     4441 cacgcgctaa agtccaattc catatatatc tatttctata atagtacaac tttaattata
     4501 attggaattc attatttcaa aagagtataa tcctcttttt tggcttctta gatgattata
     4561 tcaaataaat caaaataaga aacttcttaa gttaggtttt tcagataatg cagtatctcc
     4621 cgagaggtga gataatgcga gatagacgga caacaaagct tttaatcgcg gtgttcctga
     4681 tcgtcgggct tgtcccgata gtcggggcac tcgagacgac cccgaccacg attgtcgcgg
     4741 caagcgacag cagtgcggcc gcgaaagcgg cggctgccta tgtctgtgac ggggtcaatg
     4801 atcatgtgga gattcaggca gcactgaatg ccctgccatc atccggtggt gttgtcctcc
     4861 tttccagcgg cacatacaac tgtgctggta tcattgctcc caaagcaggc tctaccctca
     4921 agggggaggg tgagacgaag acgaaccttg tcttcaccca tgacggtcgc atcaacgtcg
     4981 accgtgagta tgtgaccctg gacgggttcg gcgtgaaggg cagcggctac agcagcggtg
     5041 tcaagt
//
LOCUS       DAOSII010000123         5044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_14828_length_5044_cov_48.917218, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000123 DAOSII010000000
VERSION     DAOSII010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14828_length_5044_cov_48.917218"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..924)
                     /gene="hemC"
                     /locus_tag="PLV90_08495"
     CDS             complement(40..924)
                     /gene="hemC"
                     /locus_tag="PLV90_08495"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOF97193.1"
                     /translation="MSLRIGTRGSALALAQTNKVAGMLADRGIETEVVTITTEGDTAT
                     GVPLHAIGGQGVFVRALDDAILRGDIDAAVHSMKDIPAARPAGLACSAVLKRDSPADF
                     LAHDLPLDAVRVVGSSSTRRRAQLLRYDPALEVKQLRGNVDTRIRKLREGQYDAIVLA
                     EAGLERLGLSLPGEALPLDQFVPSPNQGTVAVVCRDDPDIAGVFEALDHAPTRLDVMI
                     ERAVMEEVGGGCFTPQGIYCRGGDLIAEVLALDGSRRERIEQHVATPEEARACGRALR
                     GKAADLIREAYAALGLRS"
     gene            complement(909..2156)
                     /gene="hemL"
                     /locus_tag="PLV90_08500"
     CDS             complement(909..2156)
                     /gene="hemL"
                     /locus_tag="PLV90_08500"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HOF97194.1"
                     /translation="MKSSDLFARAKTLMPGGVSSPVRAIRPYPFYVERAAGSHIATVD
                     GADLIDCCLGYGPLILGHAHPVIREAIKRQLEKGWLYGTPSPLELDLAEVITGDHPGI
                     DMVRFVSSGSEATMAAIRLARGYTGKQDIVKVEGGFHGAHDAVLVKAGSGATTLGVPD
                     SAGVLADLTAHTRQVPYNDPEALEAILAGNDDVAAFILEPIMGNIGPILPDSGYLADV
                     REITAAHGVLLILDEVITGYRVGIGGAEVLYGVKPDIATFGKIIGGGLPIGAFGGRRD
                     IMDLIAPSGPVYQAGTFSGNPASLAAGIATLRHLHDHPDIYRRLDDATRAIEEVAVDA
                     GRGSFVRMGSLFKHFFRDTPPRDYREVKECNTEAFGRFWKAMLEAGIFLPPSQFETNF
                     LSATHSDQDIEQIAGAYGSCLFA"
     gene            complement(2143..3126)
                     /gene="hemB"
                     /locus_tag="PLV90_08505"
     CDS             complement(2143..3126)
                     /gene="hemB"
                     /locus_tag="PLV90_08505"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HOF97195.1"
                     /translation="MFPERRMRRIRRRNIRPLLRETELQKTDLVMPVFVDESIRTPLA
                     IASMPGQFRHPVDGVADYCERLHTAGIRAVILFGIPAAKDSEATGAYAADGVVQRAAR
                     SIKERLPQMVVATDVCACEYTDHGHCGIVGETIDGPDLKNDPSLALMARIAVSHAESG
                     ADIVAPSCMLDGMVQAIREALDAAGYQDVLIMSYSSKFASALYGPFRDAADSGFSFGD
                     RTTYQIDPGNAREAVMESELDAAEGADILMVKPAGIYLDILASVADLGLPVAAYQVSG
                     EYAMIKAAGERGWLDERAVALESLTAIKRAGADLIITYFAEDAARWLDEEQ"
     gene            complement(3105..4379)
                     /gene="hemA"
                     /locus_tag="PLV90_08510"
     CDS             complement(3105..4379)
                     /gene="hemA"
                     /locus_tag="PLV90_08510"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HOF97196.1"
                     /translation="MPDPAATTPLALAGVNHHTADIATIEAFRFPDEAAFLHEARERF
                     RGALLLQTCNRVEVLVQGDARSLEGFLAEQGRRGFAVIEGEAVPRHLLELAAGIDSLV
                     VGEDQILGQLKRALAVTEEAGAASSVIKICINKAVHVGVRVRRQTEINRGAVSVGSAA
                     VTLAEKLLGTLRDRHILVIGSGEMGLLVTQALCAKDLTAIYVANRTYERAVMLAEKIG
                     GRAVNFRDLYRYIALSDVVISCTAAPHPVIRTEDIREVMEKRLWPLDPAPRHLILIDI
                     AQPRDIEDEVRSVEGVHLFTIDDLRSINDAAMDSRRSEADRARGIIDEELDRFVRLLR
                     RTAADETLALLYTWAESIRTRERDRALARLGEEDDRTVEVIDDLTRALTKKLLSDATA
                     AIRASAECGDTARADGLMRAITRGEPCFQNDE"
     gene            complement(4372..4998)
                     /locus_tag="PLV90_08515"
     CDS             complement(4372..4998)
                     /locus_tag="PLV90_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HOF97197.1"
                     /translation="MIPLMLDLTGRRVLIFGGGDVGARKAAYFAHEAEVTVISRSFSP
                     DLDGLAIRRQETDLAALSDEALQSLLAGTFLAVAATPDAALNDRIGRVCAGSGVLFNN
                     AAGKPGDIVIPSVVRGSRYLVAISTRGKSPAVSRYIRMRFEAEYADLDRMIDLQDDMR
                     SMLKEVEPVQAERSRILWSILRDEEVWRALASDYDGARTMAIERYLHA"
BASE COUNT          803 a         1695 c         1678 g          868 t
ORIGIN      
        1 gcccgcccgg gccggacccc acaagatacg cttttcctgt catgatctca accccagtgc
       61 cgcatacgct tcccggatca ggtccgccgc cttcccgcgc agggcccgcc cgcacgcccg
      121 ggcctcttcg ggcgtcgcga cgtgctgctc gatacgctcc cggcgtgaac cgtcgagcgc
      181 gagcacctcg gcgatcaggt cgccgccccg gcagtagatc ccctgcggtg tgaaacaccc
      241 gccgccgacc tcctccatga cggctcgctc tatcatcacg tcgaggcggg tgggggcatg
      301 gtcgagcgcc tcaaagaccc cggcgatatc cgggtcgtcc cggcagacga ccgcgacggt
      361 cccctggttt ggggacggga cgaactgatc caggggaaga gcctccccgg gaaggctgag
      421 gccgagccgc tcgaggccgg cctccgcgag aacgatggcg tcgtactgcc cctcgcggag
      481 cttccgtatc cgtgtatcga cgttcccccg gagctgtttc acctcaagcg ccgggtcgta
      541 gcggaggagc tgggcgcggc gccgggtgct cgacgatccg acgacgcgga cagcgtcgag
      601 agggaggtcg tgcgcgagga aatccgccgg cgagtcacgc ttgagcaccg cagagcaggc
      661 aagccctgcc ggccgcgccg ccggtatatc cttcatgctg tgcaccgcag cgtcgatatc
      721 gccgcggagg atggcgtcgt cgagcgcccg gacaaagacc ccctgtcccc cgatggcgtg
      781 gagcgggact cccgtcgcgg tatcgccctc ggtcgttatc gtgacgacct ccgtctcgat
      841 gccccggtcg gcgagcatcc cggcgacctt gttcgtctgg gcaagcgcaa gagcgctccc
      901 ccgtgtgcct atgcgaagag acatgatccg tatgcgcccg ctatctgttc gatatcctga
      961 tcgctgtgcg tcgcggagag gaagttcgtc tcgaactgcg agggcgggag gaagatgccc
     1021 gcctcgagca tcgccttcca gaaccgcccg aatgcctccg tgttgcattc cttgacctcc
     1081 cggtagtctc gcggcggtgt atcgcggaag aagtgcttga agagcgatcc catcctgacg
     1141 aacgaaccgc gccctgcatc gacggcgacc tcctcgatcg cccgcgtggc gtcgtcgagc
     1201 cgccggtaga tatccggatg gtcgtggagg tgacggaggg tcgcaatccc cgccgcaagg
     1261 cttgccgggt tgccgctgaa cgtccccgcc tggtagacgg gtcccgaagg cgcgatcagg
     1321 tccatgatgt cgcgccgccc tccgaaggcc ccgatgggga ggccgccgcc gatgatcttg
     1381 ccgaacgtgg cgatatcggg ctttaccccg tagagcactt cggcgccgcc gataccgacc
     1441 cggtagccgg tgatcacctc atcgagtatg aggaggaccc cgtgggcggc ggtgatctcg
     1501 cggacgtcgg cgaggtagcc gctgtcgggg aggatcggcc cgatgttccc catgatcggc
     1561 tcgagtatga acgcggcgac gtcgtcgttc ccggcaagga tcgcctccag cgcctcagga
     1621 tcgttgtacg ggacctgccg ggtgtgtgcc gtaaggtcgg cgaggacgcc ggcggagtcg
     1681 ggcacaccga gggtggtcgc tcccgacccg gccttcacga ggaccgcatc gtgggcgccg
     1741 tggaacccgc cctcgacctt gacgatgtcc tgtttgcccg tgtagccgcg ggcgagccgg
     1801 atcgcggcca tcgtcgcctc cgagcccgac gagacgaacc ggaccatatc gatgccgggg
     1861 tggtcgccgg tgatgacctc cgcaaggtcg agctcaaggg gcgacggcgt gccgtagagc
     1921 cagcccttct cgagctggcg ctttatcgcc tcccggatga ccgggtgcgc gtggccgagg
     1981 atgagcggac cgtagccgag acagcagtcg atgagatccg ccccgtcgac ggtcgcgata
     2041 tgcgagcccg ccgcccgctc cacgtagaac gggtagggcc tgatggcccg gacagggctg
     2101 ctgacgccgc ccggcatcag ggtctttgcc cgtgcgaaga ggtcactgct cttcatcaag
     2161 ccacctcgct gcatcttcgg caaagtaggt gatgatcagg tcggccccgg cacgcttgat
     2221 ggcggtgagg ctctcgaggg cgactgcccg ctcgtccagc cagccgcgtt ctccggcggc
     2281 cttgatcatg gcgtactccc cgctgacctg gtaagccgcc accggcaggc cgaggtccgc
     2341 caccgatgca aggatatcga ggtatatccc ggccggcttc accatcagta tatcggcccc
     2401 ctcggcggcg tcgagttccg actccataac cgcctcgcgg gcgttgcccg ggtcgatctg
     2461 gtaggtcgtc cggtcgccga acgagaaccc cgagtccgcg gcgtcgcgga acggcccgta
     2521 gagggcgctc gcgaacttcg aggagtagga catgatcagg acgtcctggt acccggcggc
     2581 atcgagcgcc tccctgatcg cctgcaccat gccgtcgagc atgcacgacg gcgcgacgat
     2641 atcggcgccg ctctcggcat gggagacggc tatcctggcc atcagggcaa gcgacggatc
     2701 gttcttgagg tcggggccgt cgatagtctc tccgacgatg ccgcagtggc cgtggtcggt
     2761 gtactcgcag gcgcagacgt cggtagcgac caccatctgc ggcaggcgct ccttgatcga
     2821 tctcgcggcc cgctggacga cgccgtcggc cgcgtaggcc ccggtcgcct cgctgtcctt
     2881 tgctgccggt atgccaaaga ggatcaccgc ccggatgccg gcagtatgga ggcgctcgca
     2941 gtagtcggcg accccatcca cggggtgccg gaactgcccc ggcatcgagg cgatggccag
     3001 tggtgtgcgg atcgattcgt ctacaaagac cggcatgacg aggtcggtct tttgaagttc
     3061 tgtctcgcgg aggagcggcc ggatgttccg ccgccgtatc cgcctcattc gtcgttctgg
     3121 aaacatggtt ctccccgggt gatcgccctc atcaggccat cggctctcgc cgtatcgccg
     3181 cattccgcac tcgcacggat ggctgcggtc gcgtccgaga ggagcttctt cgtgagcgcc
     3241 cgcgtcaggt cgtcgatgac ctccaccgtg cggtcgtcct cctctcccag gcgggcgagc
     3301 gcccggtcgc gctcgcgtgt ccgtatcgac tccgcccagg tgtagaggag ggcaagcgtc
     3361 tcgtcggccg ccgtccgccg gaggagacgg acgaagcggt cgagctcctc gtcgatgatc
     3421 ccccgtgccc ggtcggcctc gctcctccgg gaatccatgg cggcatcgtt gatgctgcgc
     3481 aggtcgtcga tggtgaagag gtgcacgccc tcgacggacc gcacctcatc ctcgatatcg
     3541 cggggctggg cgatgtcgat gaggatcagg tggcgggggg ccggatcgag cggccagagg
     3601 cgcttctcca tcacctcccg tatatcctcc gtgcggatga ccgggtgcgg cgcggcggtg
     3661 caggatatga cgacgtcgga gagcgctatg tagcggtaga ggtccctgaa gttgaccgcc
     3721 cggcccccga tcttctccgc aagcatcacc gcgcgttcgt atgtcctgtt cgcgacgtat
     3781 atcgccgtca ggtccttcgc acagagcgcc tgggtcacca gcagccccat ctccccgctc
     3841 ccgatgacca ggatgtgccg gtcgcgcagg gtgccgagga gtttctcggc aagcgtcacg
     3901 gccgcggagc cgacagagac cgcaccccgg ttgatctctg tctggcgccg gaccctgact
     3961 cccacgtgca ccgccttgtt gatgcagatc ttgatgacgc tgcttgcggc ccccgcctcc
     4021 tccgtgaccg cgagcgcccg tttgagctgg ccgaggatct ggtcctcgcc gacgacgaga
     4081 gagtcgatgc cggcagcgag ttccaggaga tggcggggca cggcctcccc ctcgatgacc
     4141 gcaaaaccgc gccggccctg ttccgcaaga aacccctcaa ggctccgcgc gtcgccctgc
     4201 accaggacct cgacgcggtt gcaggtctgg agcaggaggg cgcccctgaa ccgctcccgg
     4261 gcctcgtgga ggaaggccgc ctcgtcgggg aaccggaacg cctcgatcgt ggcgatgtca
     4321 gcggtgtggt ggttcacgcc cgcaagagca agcggggtcg ttgcggccgg atcaggcatg
     4381 caggtacctc tcgatcgcca tcgtgcgggc cccgtcgtag tcggaggcaa gcgctctcca
     4441 gacctcttcg tcgcggagga tgctccagag gatacgggac cgctcggcct gcaccggttc
     4501 gacctctttg agcatcgagc gcatatcatc ctgcaggtcg atcatccggt cgaggtccgc
     4561 atactcggcc tcaaaccgca tgcggatgta gcgcgatacg gcagggctct tcccgcgggt
     4621 gctgatcgcg accaggtagc ggctgccccg gacgaccgaa gggatgacga tatcgccggg
     4681 tttaccggcg gcgttgttga agaggacgcc ggatccggcg catactctcc cgatacggtc
     4741 gttgagtgcc gcatccggcg tcgcggctac cgcgaggaag gtgcccgcga ggaggctctg
     4801 gagcgcctcg tccgaaaggg cggcaagatc ggtctcctgc cgccggatcg caagaccgtc
     4861 aaggtccggc gagaatgaac ggctgatcac cgtcacctcc gcctcatgcg caaagtatgc
     4921 agccttccgg gcaccgacgt cgcctccccc gaatatgagc accctcctgc ccgtcaggtc
     4981 aagcataaga gggatcattc ttctatagtg tggtgaacgg aatagattaa ggtgttgaaa
     5041 aacc
//
LOCUS       DAOSII010000124         5021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_14910_length_5021_cov_51.547322, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000124 DAOSII010000000
VERSION     DAOSII010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14910_length_5021_cov_51.547322"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PLV90_08520"
     CDS             complement(<1..531)
                     /locus_tag="PLV90_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOF97198.1"
                     /translation="MPPTNSIGCSTCCLMDRTLEEALSLVSRYTGLAEILSDGPHDLF
                     GEEETCSSFDLRYTVHAPVADINIASENEHLRRASVRVVSDLADLCDRIGAGRLVIHP
                     GHTWGEEMRGVAERALNRSLSDLATIQQDVNVRLALENMGAWDICFFREPGFLARLAD
                     GGLGFALDVGHAHVNGN"
     gene            complement(541..1653)
                     /locus_tag="PLV90_08525"
     CDS             complement(541..1653)
                     /locus_tag="PLV90_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOF97199.1"
                     /translation="MGWCRMRRNLIVPTLFAIIAVGNFVCPATGQESSGDPIWESGVG
                     DRVMAAALSPDGLYGAVTTERMVYLYSQNGTTLWSYPVSGGRSVAVSSGGERIAAGGD
                     HLLLFDRNGSVIWRYRSESRIQDIAITADGRTICTGADTGLLVFSLDDGEATPDVSGS
                     FDANDPIRSVSIDSDGAGIVAGADSGNVYFLGADGRLLWNYRTGDNGIHVAISRDGST
                     IAAASTGRTVYLLNRNGRLLWKSSMPRAITDVSISGDGSRLVLADGGISMLNRGGEII
                     GMHATGEEGRIRSVSAPSDTAHILAGAADGTVSMFRVQPETSPPDTATEPSRPPAAPA
                     QEQTTSQQDTALPPATPVIVSACAVAALAWRRRERR"
     gene            complement(1655..3628)
                     /locus_tag="PLV90_08530"
     CDS             complement(1655..3628)
                     /locus_tag="PLV90_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOF97200.1"
                     /translation="MKRIGSILLILLLSIATCGTAAAYPYGAGDAIVQDGLDYIRSCQ
                     HDDGGFAEAGRETNPGTSWFAVMAIVAAGEDPHNWRVNGTSAIDYWRSAPDAINPEGT
                     AELGKMVTLVAAAGENPRAFNNHDYVAELRARMKTDGQFGDFVYTTYWGVFGLASAGE
                     DTALPVAWLKKQQNEDGGYGWMAGAESDSDDTAAVIMALVAAGEPADTPAVARAIDYL
                     GAHQMNDGSFNYGGTSSSNAASAAWVIQAIAATGEDPSSWSKNGNDPVTTLVTLRQPD
                     GSFLWTTYTADNPCGMTARAIPALLGKPYPILPGQAAPDLQEPGRSAIASPGATTVPQ
                     TTVPAAGSVAWQPVTVTDDYGEDVGLDTMPVRIVSLAPANTEILFALGLGDRVVGVTD
                     YCNYPAEATTKPKVGGYSTVNIERVVAAHPDLVMAAFGNTEEVVNRLRDLGLTVITLS
                     PDSVEGTLHDIRLIGKATGAETEAERLATSMEARIDAVKEKTKSVAKPPTVAHIVWHD
                     PIWISGSNTFQDELITLAGGVNAFPDTEGWQIASLERFIATDPEVIVVNSGTGMGTEG
                     VDLIYRYFMNEPRLKTMKAIQNNRVYIIESDLIDRGGPRLVDALEEVAADIHPDLFDA
                     DIRKNAPIPQSPGFGVIPLAFAFLAVLLIRLKR"
     gene            complement(3625..>5021)
                     /locus_tag="PLV90_08535"
     CDS             complement(3625..>5021)
                     /locus_tag="PLV90_08535"
                     /inference="COORDINATES: protein motif:HMM:NF025833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HOF97201.1"
                     /translation="SNPETAHAAVTITVSIPAPESGGGGDGGGDSSPSWITVTLEPGT
                     FTLTADNSKKTYDVDRQTALGALDAAGVGYTVDDSYYQQYGSLFIDSVNGRANEGTKG
                     WMYQVNGESPGSGANTHTVGDGDKIVFFWSESMSSTPATSPDVISIKVVIPASSGGSD
                     SDSSGEGSGATPSSTGEQPNNGETGSLSFSLGLPVGVTVDLGEWGQTFSVNTTGTTAA
                     GETVVVSGNTLTINQSGLVLTVAAKNIDEKEGVARGLIESVTAAINPIAREIEGLGTI
                     TASLILNLTGIPAADGRLDVAFNATPDAAAGNAFALAAAENGKEITALAYTMTVTRTN
                     LENGEDIAGAVIRMTISPGWVEEHGGADAIRIARSAEDGTYQVLETRVVGTDESGNLI
                     VEAVSPGGLSTFGLLAVRAAQDTHATAATSAAPGASSAAGTPAEASPLIISAIGAIPV
                     IGTAYLIARRRREQ"
BASE COUNT          811 a         1543 c         1646 g         1021 t
ORIGIN      
        1 gttgccgttg acgtgggcgt ggcccacgtc gagggcgaac ccgagaccgc cgtcggcaag
       61 gcgagcgagg aacccgggct cgcggaagaa gcatatgtcc cacgctccca tgttctcgag
      121 agccaaccgg acgtttacgt cctgctggat ggtagcgagg tcgctgagcg atcgattgag
      181 ggcccgctcg gcgacgcccc gcatctcctc cccccaggta tgccccgggt ggatgaccag
      241 tcgtccggca ccgatacggt cgcagaggtc tgcgagatcg ctgacgactc tcacggatgc
      301 cctccgcagg tgctcgttct cggaggcgat gttgatgtcg gcgaccgggg cgtggaccgt
      361 ataccggagg tcgaacgaac tgcaggtctc ttcttctcca aaaaggtcgt ggggaccgtc
      421 ggagaggatc tcggcaagcc cggtgtaccg ggagacgagg gagagcgcct cctcaagcgt
      481 ccggtccatc aggcagcagg tggagcaccc gatagaattc gttggaggca ctgtattcta
      541 ctacctcctc tcccttcgcc gccacgccag cgcggcaacg gcgcacgcac tcacgatgac
      601 cggcgttgcc ggggggagcg cggtatcctg ctgcgaggtt gtttgctcct gagcgggagc
      661 ggcgggcggt cgagacggct cggtggcggt atccggcggg gaggtttctg gctgcaccct
      721 gaacatcgat acggtcccgt cggccgcacc ggcgagaata tgcgcggtat cggatggggc
      781 ggagacggac ctgatccgcc cttcttctcc cgtcgcatgc atccctatga tctctccgcc
      841 ccggttgagc atggagatgc ccccgtccgc cagcacaagt ctcgatccgt ctccggatat
      901 cgatacgtcc gtgatcgccc ggggcatcga agacttccag agaagccgcc cgtttcgatt
      961 caggagatac accgttcgcc cggtcgatgc tgcggcaatt gtcgaaccgt cgcgcgagat
     1021 ggccacgtga atgccgttat cgcctgtcct gtagttccag agaagccggc catctgcgcc
     1081 gaggaagtag acattccccg aatcggcacc ggcaacgatg ccggcaccat cggagtcgat
     1141 cgatacgctc ctgatcgggt cgttcgcatc gaacgacccg gagacgtcgg gggttgcctc
     1201 tccgtcgtcc agggagaaga ccagaaggcc ggtatccgcc ccggtgcaga tcgtacgtcc
     1261 gtctgccgtg atagcgatat cctgtatccg cgactccgat ctataccgcc agatcacgct
     1321 gccgttccgg tcgaagagca ggagatggtc ccctcctgcc gcgatgcgct ctccaccgga
     1381 tgatacggcc acgcttctgc cgccggatac cgggtaactc cagagtgtcg tgccgttctg
     1441 gctgtagaga tagaccatcc tctctgtagt gacggcaccg tagaggccgt ccggcgagag
     1501 tgccgccgcc ataacccgat caccgactcc tgactcccag atcggatcgc cggaggattc
     1561 ctgccccgta gccgggcata cgaagttccc gaccgcaatg atcgcaaaga gcgtgggtac
     1621 gatgaggttt cgccgcattc tgcaccagcc catcctacct cttcagccgg atcaggagga
     1681 cggcgaggaa cgcaaaagca agcgggatca cgccgaaacc tggagattgc ggtataggag
     1741 cattcttccg gatatctgca tcgaagaggt cggggtggat gtcggcagcg acctcctcca
     1801 gggcgtcgac cagacgcggc ccgccccggt cgatgagatc ggactcgatg atgtagacac
     1861 ggttattctg gatcgccttc atggttttga ggcgcggctc gttcatgaag tagcggtaga
     1921 tgaggtcgac gccctcggtg cccatgccgg ttccggagtt gacgacgatc acctcgggat
     1981 cggtggcgat gaaccgctcg aggctggcga tctgccagcc ctccgtatcg gggaaggcgt
     2041 tgacgccgcc ggcaagggtg atcaactcgt cctggaaggt gttgctcccg ctgatccaga
     2101 tcgggtcgtg ccagacgata tgggcgaccg tcggaggttt ggccacgctc ttcgtttttt
     2161 ccttcacggc gtcgatccgt gcctccatcg atgtcgccag ccgctccgct tctgtttctg
     2221 cgccggtggc ctttccgatg agcctgatat cgtggagcgt cccttcgacc gagtccgggc
     2281 ttaaggtgat cacggtcagg ccgagatctc gaaggcgatt gacgacctct tctgtgttcc
     2341 cgaaagcggc cattaccagg tcgggatgcg cggccaccac gcgttcgatg ttcacggtgg
     2401 agtacccgcc gacttttggt ttcgtcgttg cctctgccgg gtagttgcag taatcggtca
     2461 cgccgaccac ccggtcgccg aggccgaggg cgaagaggat ctcggtgttg gccggggcaa
     2521 gggagacgat gcgcaccggc atcgtatcga gcccgacatc ctcgccgtag tcgtcggtga
     2581 cggtgacggg ctgccacgcc acggagccgg ctgccggtac ggttgtctgc ggcaccgtcg
     2641 tcgccccggg cgatgctatc gcggatcgcc ctggttcctg gagatcgggt gccgcctgcc
     2701 cgggcaggat cgggtagggt ttgccgagca gggccgggat cgcccgcgcc gtcatgccgc
     2761 aggggttgtc ggcggtgtat gtcgtccaga gaaacgagcc gtcgggctgc cggagggtta
     2821 caagtgtcgt gaccggatcg ttgccgttct tcgaccatga cgacgggtct tccccggttg
     2881 cggcgatcgc ctggatcacc catgccgccg aggcggcgtt cgaggaggaa gtcccaccgt
     2941 agttgaagct gccgtcgttc atctggtgtg ccccgaggta gtcgatcgcc cgtgcgacgg
     3001 cgggtgtatc tgccggttcg cctgcggcga cgagggccat gatcacggcg gcggtgtcgt
     3061 cggagtcgct ttcggcgccc gccatccagc cgtagccgcc gtcctcgttc tgctgcttct
     3121 tcagccaggc gacggggagc gcggtgtctt cgccggctga ggcgagcccg aagactcccc
     3181 agtaggtggt gtagacgaaa tcgccgaact ggccgtcggt tttcatgcgt gctctgagtt
     3241 cggcgacgta gtcgtggttg ttaaaggcgc gggggttctc gcccgcggcc gcgacgaggg
     3301 tgaccatctt tccgagctcg gctgtacctt cgggatttat ggcgtcgggt gccgatctcc
     3361 agtaatcgat cgccgatgtg ccgttcaccc tccagttgtg cgggtcttct ccggcagcga
     3421 cgatcgccat cactgcaaac catgaggtgc cgggattcgt ttccctcccg gcctcggcaa
     3481 acccgccgtc atcgtgctgg catgacctga tgtagtcaag accgtcctgc acgatcgcat
     3541 cgcccgctcc gtaggggtag gccgccgccg taccgcaggt ggcgatgctg agcaggagga
     3601 tgagcaggat gctgcctatc cgtttcattg ctctctcctc cgcctggcga tgaggtatgc
     3661 cgtcccgatg acggggatcg cgcctatcgc agatatgatg agcggcgatg cttctgcagg
     3721 cgttcctgct gctgaggatg cgccgggggc cgcagaggtc gcggcggttg cgtgggtatc
     3781 ctgtgccgct ctgaccgcca gcagcccgaa ggttgagagg ccgccgggcg agaccgcttc
     3841 gacgatcagg tttccgctct cgtcggtccc gacgacccgg gtttcaagta cctggtacgt
     3901 gccgtcctct gcggatcgtg cgatcctgat agcatccgcg ccgccgtgct cttcgaccca
     3961 cccggggctt atggtcatcc ggatcactgc tccggcgata tcctcgccgt tctccaggtt
     4021 ggtccgcgtg acggtcatgg tgtaggcaag ggcggtgatc tctttaccgt tctctgcagc
     4081 ggcaagggcg aaggcgtttc ctgctgctgc gtccggggtg gcgttgaagg cgacatcgag
     4141 ccggccatcg gccgccggga tgccggtgag gttgaggatc agtgaggcgg ttatggttcc
     4201 cagcccctcg atctctcttg cgatcgggtt gatcgccgca gtgacgctct cgatcaggcc
     4261 tcttgccacg ccttcttttt cgtcgatgtt ctttgcagcg accgtcagca cgaggccgct
     4321 ctggttgatc gtgagggtgt tgccggatac gacgaccgtc tcgccggctg cggttgttcc
     4381 ggtggtgttg accgagaagg tctggcccca ctccccgaga tcgacggtga caccgaccgg
     4441 caatccaagg gaaaacgata gagatcctgt ttcaccgttg ttcggctgct ccccggtgga
     4501 ggagggcgtt gcaccggaac cttctcctga agagtcggag tcagaaccac cggaagatgc
     4561 cgggatcacg accttgatag agatgacatc cggagaggtc gccggggtcg aactcatgct
     4621 ctcgctccag aagaagacga tcttgtcgcc gtccccgacc gtgtgcgtat tggcaccact
     4681 gccggggctc tcgccgttca cctggtacat ccagcctttt gtcccttcgt ttgctcttcc
     4741 gttgacggag tcgatgaaga gcgacccgta ctgctggtag taggagtcgt cgacggtata
     4801 cccgactccg gcagcatcga gggccccgag tgccgtctgc cggtcgacat cataggtttt
     4861 tttgctgtta tcggcggtga gggtgaacgt cccgggctca agggtgacgg tgatccagga
     4921 cggcgatgaa tcgccgccgc catctccgcc accgccgctc tctggtgccg ggatggagac
     4981 ggtgatggtg accgcagcat gggcggtttc agggttcgat c
//
LOCUS       DAOSII010000125         5006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_14965_length_5006_cov_54.714401, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000125 DAOSII010000000
VERSION     DAOSII010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14965_length_5006_cov_54.714401"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..483
                     /gene="rpl7ae"
                     /locus_tag="PLV90_08540"
     CDS             115..483
                     /gene="rpl7ae"
                     /locus_tag="PLV90_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7Ae"
                     /protein_id="HOF97202.1"
                     /translation="MAKAYVKFEIPAEIQNKALEALEIARDTGKVKKGANEATKAIER
                     NIAQLVLIGSDVEPEEIVMHLPPLCEEKQIPFVYITKQNDIGSASGLEVGSAAAAIVK
                     PGKAKDLVDEIAKQITALRA"
     gene            494..703
                     /locus_tag="PLV90_08545"
     CDS             494..703
                     /locus_tag="PLV90_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S28e"
                     /protein_id="HOF97203.1"
                     /translation="MANDGTPAEVIEIIGMTGMHGEAQQVKCRVLDGPNKGRIITRNT
                     VGPIREGDILSLLETEREAKKLSRR"
     gene            709..900
                     /locus_tag="PLV90_08550"
     CDS             709..900
                     /locus_tag="PLV90_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24e"
                     /protein_id="HOF97204.1"
                     /translation="MVDRYICNFCGEPLEPGTGKMFVRRDGTVYYFCSTKCQNNYRLG
                     RVPRRVEWTAAGRKARGKE"
     gene            907..1356
                     /gene="ndk"
                     /locus_tag="PLV90_08555"
     CDS             907..1356
                     /gene="ndk"
                     /locus_tag="PLV90_08555"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOF97205.1"
                     /translation="MDRTFVMVKPDGVQRGLVGEIVSRFEAKGLKLAGAKLELLPEGR
                     VVEQYREHLEKPFFPGLKAYIMSGPCFLMAWEGKGAVAVVRRMVGATNPAEAAPGSIR
                     GDFALDIGRNVIHASDSPESAARELGIHFSPRELVEYSRIDEPVLYE"
     gene            1556..2209
                     /locus_tag="PLV90_08560"
     CDS             1556..2209
                     /locus_tag="PLV90_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter"
                     /protein_id="HOF97206.1"
                     /translation="MADLLSFALLSFSSIVIVVNPLAATLIFVSLTGTMDGATKLKVA
                     RDASRFALVVLLIFTVAGGWILQLFGISIEAFRIAGGLLLFGIGMDMVYAKTSRTKMT
                     ATEKYEGQDADDIAVMPLAIPMITGPGAITSAIVLMNEAMSMGIAALAVVPIAAGAAI
                     AITYYMMQHSDIIVRRIGQREYRAVNRLMGMLLIAIAIQFILIGIRAAFPILTGGPA"
     gene            2206..2409
                     /locus_tag="PLV90_08565"
     CDS             2206..2409
                     /locus_tag="PLV90_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97207.1"
                     /translation="MTPDLTFPGMVLISSSIIVGGILIGALTFAIDVPYASCTMVVLA
                     ALIVLGATMLVLSTRNQGGVPSR"
     gene            2409..3188
                     /locus_tag="PLV90_08570"
     CDS             2409..3188
                     /locus_tag="PLV90_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOF97208.1"
                     /translation="MTKIALAQVLGGAARALDTAERMAGEAALAGASLICFPEQFVAG
                     WLPEIPPGSGEPLDGPLATAFGRMARESGIAVAGSIIEARGGGRPQNTVVILDANGEL
                     LATYAKIHLFSPGGEDRNYTPGDRVATFTIDGMTFGLAICYDLRFPEIFRIYALAGAE
                     CVLLPAAWPCRRLRHWETLIPARALENQYYVAGVNTVGGVYCGGSLAAGPDGDTIARG
                     GSGEELLFVDLDPAAIHEARMRLPTFSDRRSDLYHRLLSNL"
     gene            3198..3941
                     /locus_tag="PLV90_08575"
     CDS             3198..3941
                     /locus_tag="PLV90_08575"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOF97209.1"
                     /translation="MRGDQHVYLSLTTAGLLIAPFVPVLDPALIVTLLFGVFVGSLAP
                     DADAVDAAIFNGWIGGAKGKRGQVLNGVAVVLPVFGYTIRYLIYYPLSLVFSLILRKS
                     YRHRHRGLLHSLAGVGLTSVILSVYLAFILTWIGWSPALLPAFGVAFFAGCILHLTED
                     ACTPAGVAWLYPFSRRRMAGRIRPWGTFEIRPAAFAAVLALAAAGVFIAPFVTAVSPE
                     VLRCLALAGTLVLWLLFMLVCRVRCERRR"
     gene            4000..>5006
                     /gene="thrC"
                     /locus_tag="PLV90_08580"
     CDS             4000..>5006
                     /gene="thrC"
                     /locus_tag="PLV90_08580"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOF97210.1"
                     /translation="MYRLVCVHCGATYTPDQIIYTCNRCGHLLAVEYDLDGIDVSRDE
                     WNRRPLSVWRYRELLPVGGEPISLQEGGTPLYHLKNIGKELGLPELYAKHEGMNPTGS
                     FKDRGMTVGVSMARELGMTSVACASTGNTSASLAAYAAKAGIPCVVLLPAGKVAVGKV
                     AQALMHGARVISIRGNFDRALEMVHELCISQGLYLLNSVNPYRLEGQKTIGFEAIDQL
                     GGDVPDRMVLPVGNAGNISAVYKGLRELEALGFIDRLPMMTGIQAAGSQPLVKAIAEN
                     LDELIPEPAPETVATAIRIGAPVNAEKALVAVRATGGTAAAVTDEEILAMQRDLARKE
                     G"
BASE COUNT          958 a         1621 c         1498 g          929 t
ORIGIN      
        1 cgaatgacaa acctttatca ctcctcctat tgacataata cagcgcattc gatcagctac
       61 gctactgatg aatgatgacg gagcagcgag acctgctcct gaaagaggcg agttatggca
      121 aaggcatatg taaaattcga gattcccgca gagatccaga ataaggccct tgaggccctt
      181 gagatcgcaa gagataccgg taaggtaaag aagggagcaa acgaggcaac gaaagcaatc
      241 gagagaaaca ttgcccagct ggttctcatc ggttcagacg tcgagcccga agagatcgtg
      301 atgcatctgc cgccgctctg tgaagagaag cagattcctt tcgtctacat cacgaagcag
      361 aacgacatcg gttctgcaag cggccttgag gtcggctcgg cagccgccgc catcgtcaag
      421 cccggcaagg caaaggatct cgtcgatgag atcgcaaagc agataaccgc actgagagcg
      481 tgagggagaa gagatggcaa acgacggaac gcccgcagaa gtcatcgaga tcatcggcat
      541 gaccggtatg catggggaag cccagcaggt gaaatgccgt gtcctcgacg gtccgaacaa
      601 ggggcggatc atcacccgta acaccgtggg ccccatcagg gagggagaca tcctctcgct
      661 gcttgagacc gagcgtgaag caaagaaact ctcgaggcgg taacacccat ggtcgacaga
      721 tacatctgca atttctgtgg agagcctctc gagcccggca ccggcaagat gttcgtcagg
      781 agagacggga ccgtctacta cttctgcagc accaagtgcc agaacaacta caggctcggc
      841 cgggtgccgc gtcgcgtcga gtggacagcg gccggtcgga aagcccgcgg caaagagtga
      901 tccggcatgg atcgcacctt tgtgatggtc aagcccgacg gtgtccagcg cggactcgtc
      961 ggggagatcg tctcccggtt cgaggcaaag ggcctcaaac tcgcgggggc aaaacttgag
     1021 ctcctccccg agggacgggt cgtcgagcag taccgggaac acctggagaa accgttcttc
     1081 ccgggcctta aggcctacat catgagcggc ccctgcttcc tgatggcctg ggagggaaaa
     1141 ggcgcggtcg cggtcgtccg caggatggtc ggtgccacaa accctgcaga ggcggctccg
     1201 ggctccatac gcggcgattt tgctctcgac atagggcgga acgtgatcca cgcatccgac
     1261 tcccccgaga gtgccgcccg cgagctcggc atccactttt cgccccgcga actggtcgaa
     1321 tactcgagga tcgacgagcc cgtcctctac gagtgaatac cggcggaaaa accgttttcc
     1381 tctttttgag gtccggcgag caagatccgg gcggccgctt cggcccaaaa gaggagatct
     1441 tcttcgcctg gtggaaagca tctttctctg ttccgcgttt ttcccgaaac aaatcgtttg
     1501 tcctgccggc aaaccacaag tgctaatatc aaacctaccg catgcctgat cacaaatggc
     1561 cgatctcttg agttttgcgc tgctgagctt ctcttcgatc gtcatcgtag tgaacccgct
     1621 cgcggccacc ctgatcttcg tctccctcac cgggacgatg gatggggcaa caaaactcaa
     1681 ggtcgcccgc gatgcgagcc ggttcgccct ggtcgtcctg ctgatcttca ccgtcgccgg
     1741 cggctggatc ctgcagctct tcggcatatc gatcgaggcg ttccgcatcg cgggcggcct
     1801 cctcctcttc gggatcggca tggacatggt ctacgcaaag acatcgcgga cgaagatgac
     1861 cgcgaccgag aagtacgagg ggcaggatgc agacgacatc gcggtcatgc cgcttgcaat
     1921 cccgatgatc acggggcccg gcgccatcac ctccgcgatc gtgctgatga acgaggcgat
     1981 gagcatgggt atcgccgcac tcgccgtcgt gccgatcgcc gccggcgcgg cgatcgcgat
     2041 cacctactac atgatgcagc actccgatat catcgtccgg cggatcgggc aacgagagta
     2101 ccgggccgtc aaccggttga tgggtatgct cctcatcgcg atcgcgatcc agttcatcct
     2161 catcgggatc agggcggcgt tccccatact caccggaggc cctgcgtgac acccgatctt
     2221 acgtttcccg gcatggtcct gatcagttcg agcatcatcg tgggcgggat cctcatcgga
     2281 gctctgacgt tcgcgatcga cgtcccgtac gcctcctgca ccatggtcgt gctcgccgcc
     2341 ctcatcgtcc tcggcgccac catgctcgtg ctctccacaa gaaaccaggg cggggttccc
     2401 tcccggtgat gacgaagata gcgcttgccc aggttctcgg cggtgccgcc cgggcactcg
     2461 atacggccga acggatggcc ggggaggccg ctctggccgg ggcgtcgctc atctgttttc
     2521 ccgagcagtt cgtggccggg tggttgccgg agatccctcc cgggtccggg gaacccctgg
     2581 atggaccgct cgccacggca ttcgggcgga tggcacggga gagcggcatc gcggtcgcgg
     2641 ggtccatcat cgaggcgcga gggggaggcc ggccccaaaa taccgtcgtg atcctcgacg
     2701 cgaacggcga gctcctcgct acctatgcga aaatccacct tttctcgccc ggcggggagg
     2761 accgcaacta cacgccgggc gaccgggttg ccacgttcac catcgacggg atgacgtttg
     2821 ggcttgctat ctgctacgat ctccggtttc ccgagatctt ccgaatatat gcactcgccg
     2881 gggcggaatg cgtgctcctg ccggcggcat ggccgtgccg ccggttgcgc cactgggaga
     2941 ccctgatccc cgcacgcgcc ctcgagaacc agtattacgt ggcaggagtc aacacggtcg
     3001 gcggggtcta ctgcggggga tcgcttgccg caggccccga cggcgatacc atcgcccggg
     3061 gcgggtccgg ggaagaactc ctcttcgtcg acctcgaccc ggccgcgatc cacgaggccc
     3121 gcatgagact cccgacattt tcagaccgca gaagcgacct ttatcacaga ttgctctcta
     3181 atctatagag acatctcatg cggggcgacc agcacgtata cctcagtctc acgacggcag
     3241 gactcctcat cgcacccttc gtccccgtcc tcgacccggc cctgatcgtc accctcctct
     3301 tcggtgtctt tgtcggttcg cttgcaccgg atgcggatgc cgtcgatgcg gcgatcttca
     3361 acggctggat aggcggggcg aaggggaaga gaggacaggt cttaaacggt gttgccgtag
     3421 tgctcccggt ctttggctac accatccggt acctcatcta ctacccgctc tcccttgtct
     3481 tctcgctcat cctccgaaag agttaccggc accggcaccg cggtctgctc cactcgctcg
     3541 cgggcgtcgg gttgacatcc gttatcctct ccgtctacct ggcgttcatc ctcacctgga
     3601 ttggatggtc tcctgcactc ctccccgcct ttggagttgc gttcttcgcc gggtgcatcc
     3661 tccatctcac ggaagatgcc tgtacacctg ccggcgtcgc ctggctctac cccttctccc
     3721 ggcggcggat ggccggccgc atccggccat ggggaacctt cgagatacgc ccggcagcct
     3781 tcgccgccgt gctcgccctg gcggctgccg gcgtgttcat cgcgccattc gtgaccgccg
     3841 tatcgcccga agttctcagg tgcctcgccc tcgccggaac cctcgttctc tggctgctct
     3901 tcatgctcgt ctgccgcgtc cggtgcgagc ggcgcaggtg agccggcccc gatccgccat
     3961 accatatcta tacgtatccg ggcgatcaac catttttcca tgtaccgtct cgtctgcgtt
     4021 cactgtggtg caacctacac cccggaccag atcatctata cgtgcaaccg ttgcgggcac
     4081 ctgctggccg tcgaatacga cctggacggc atcgacgtct cccgcgacga atggaaccgg
     4141 cgccccctct cggtctggcg ttaccgcgag ctcctcccgg tcgggggcga acccatcagc
     4201 ctccaggagg ggggaacccc gctctatcac ctgaagaata tcggcaaaga actgggcctc
     4261 cccgaactct acgccaaaca cgaggggatg aacccgacag gatcgtttaa ggatcgcggc
     4321 atgaccgtgg gcgtcagcat ggcccgggaa ctcggtatga cgagcgtcgc ctgcgcaagc
     4381 accgggaaca cctccgcgag cctcgccgcc tacgcggcga aggccggcat cccctgcgtc
     4441 gtcctcctgc ctgcgggaaa ggtagccgtc ggcaaggtcg cgcaggcgct gatgcacggc
     4501 gcacgggtca tatccatccg ggggaacttt gatagggcgc ttgagatggt acacgaactc
     4561 tgcatcagcc agggcctcta cctcttgaac tcggtcaacc cctaccgcct ggaggggcag
     4621 aagacgatcg ggtttgaggc aatcgatcaa ctcggcggcg acgtgcccga ccggatggtc
     4681 ctccccgtcg gcaacgcggg caacatatcc gcggtctaca agggtctccg agaactcgag
     4741 gccctcgggt tcatcgaccg cctgccgatg atgaccggca tccaggcagc cggctcgcag
     4801 ccgctcgtga aggcgatagc agagaacctc gacgaactca tccccgaacc cgcgcccgag
     4861 acggttgcga ctgcaatccg gataggcgcc ccggtgaacg cggagaaggc gctcgtcgcc
     4921 gtccgcgcga ccggcgggac ggcggcggcc gtgaccgacg aagagatcct tgccatgcag
     4981 cgagacctcg cgaggaagga gggcat
//
LOCUS       DAOSII010000126         4993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_15018_length_4993_cov_45.766707, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000126 DAOSII010000000
VERSION     DAOSII010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15018_length_4993_cov_45.766707"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PLV90_08585"
     CDS             complement(<1..472)
                     /locus_tag="PLV90_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HOF97211.1"
                     /translation="MLGTVPGIDPEVLSLVIVPAFIFFARICDVTIGTMRIIFVSRGM
                     KMVAPVLGFFEVFIWIIAVGQIFQNLTNPLNYFAYAAGFATGNYVGMLVEERLAMGLA
                     LIRVITQRDATNLIDYLRGAGYGVTVLDAQGKQGPGKVIFSVIKRKNIKDVEKAI"
     gene            1154..>4993
                     /locus_tag="PLV90_08590"
     CDS             1154..>4993
                     /locus_tag="PLV90_08590"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOF97212.1"
                     /translation="MLIAPAAADSLPDRVYVNASYNGETPGYGTLNFSSIQEAVDNVT
                     DGGEVWVYNGTYNEPIVIDRAMTVTTVSHLECPGLDGVIIDAMGEQTGIEIDADEVSI
                     SGFEVVNASLFGIYAHNAGAVTIEYNSVTVENDTYELTCGILIEGGEGVCIGNNAVRV
                     IGSSAQAGIMVNGVTEANVHDNHVVAVSRHQGLEAVEMEPNPVVAALFTEDNEPFIAA
                     RATVTQQASAGVIIEESSSVLVERNRIVAVGNCTEDALDLHYSAVEAWGIGSGGNSKN
                     LKILENDVTVVGKALDTAWTVGILGVGDLALVQSNTVEVIARSRVVLPVGIVISEAEK
                     GQIFDNKIEVEAACIGKSDGEVSPEATGIIAVESPEAQVLRNDVLYTLTLTDAGQLAM
                     TSTEGIYVGGCDESRVSENIVLIQTGIFNLPVDGDESTEDEESLTLGVARVKGIGVEA
                     SDKPEICDNVVHAFGAVMTYDETAGDLNVGALSALVMTGIHVWSYSDDLLENPVVREN
                     VVVVRAQVQTNLVPELELDEEEVKAALAGNSALLARYVGMMQESEEFSVATCLADTEG
                     LSALTESDLEFEPDIDEATLAAALTVTAGIVLEDVLNPEVCCNYVPVYQDVAVIVFDR
                     PVDDVGMVLQTPLSLGLIDQAFSDEQVQQAFLLGLIDEDESAWQNLSKDDRAAITQAV
                     LAGDTGVLAAYTDDETLAGYQALMEDGEAFEFLNTTRLAAAIPLSVSCGVYVNDAEGD
                     VVLHDNEIVVETNAAGLAAAQGGDLDAPDAAAGSAGIVAAFGFAGTGDSISVEENCIH
                     VITNGMFLGQAMGGENETADTAAGTANLFLGVGVLAGADGEHVVNNDITVAQRSRAQT
                     LALNIVKDRTALSVAATAGVGIGIVLDPYEVFDPETYEPAPPAPDEWFASVIEGNTIA
                     VTNDINIFSLAEMLLPAPLDGSSNALSGAAGAAASFGIVAPEADVLDNVIEIHAVQSG
                     YAGAFVEERPIILAQSAEIDLPGAGAANLGVAVSNGILTVRSYIENNTVYTAANSEAS
                     VEASTEELLEDAGVLAGAVAADIGIVSLSPSYIDGNTVDGDLSMALITMVNGDRVDGD
                     VLFLGLDLGILSVGSDATFNNIHNGYMGFPYYYDDEEYEPYADYNWWGDASGPSGLGP
                     GTGSPVVGTMNYEPWLTRPADVVLATGKSYFGLEIGSPNIGGDGYRSGLEPGWTTLSF
                     PLALENSTWQAVTHTGGGLDYSVAWTWDAAGQKWVQMTATSTINPLDAVYIRMNDYDR
                     LPVAISPEITSPPVKMLR"
BASE COUNT         1036 a         1539 c         1512 g          906 t
ORIGIN      
        1 ggatggcctt ctccacatcc ttgatgttct tcctcttgat caccgagaag atgactttgc
       61 cggggccctg ttttccctgg gcgtcgagca ccgtcacccc gtagccggca ccgcggaggt
      121 aatcgatgag gttcgttgca tcccgctggg tgatgacccg gatgagggca agccccatcg
      181 ccagcctctc ctcgacgagc atcccgacgt agtttccggt cgcaaacccg gccgcgtagg
      241 caaagtagtt gagcgggttc gtgaggttct gaaagatctg gccgacggca atgatccaga
      301 tgaagacctc gaagaacccg aggaccgggg cgaccatctt catcccccgc gagacgaaga
      361 tgatccgcat cgtcccgata gtgacgtcgc agatccgcgc aaagaagata aacgccggga
      421 ctatgaccag ggataagacc tccgggtcga tacccgggac ggtaccgagc atggatcatt
      481 gttgatgaac gagaaatata attctatcca tatgtaaata cataccgtcg agaaaaagct
      541 catgaagtcg cgctttcgag gatattgacg gataattaca gactatgccg gtggaatgaa
      601 gttgagatcc ttttgtaggc atagcacccg ccgggcatct ttatttaccc ggactctctc
      661 gatctcttcg tcgacctcgc cggtgctctc ctcccccgga ggttcggttc gacgaacaac
      721 cgggagggtt cgtcgacatg tgctcccatc ggtgatatcc ctgaagatcc cacgggcagg
      781 ctcgttcccg gcggcgtcgg tcatccggtt gtaaatctgg acggttccaa ttatgccggg
      841 gtctaccggc atgcggcagg aggagggagg tcacggcctc tgccgggatt cagggtgccg
      901 gccgtcgcgc ggggggaagg ggtcgccggc atagcatcga tgaaatcgaa cttttttcaa
      961 aaaatcaggc aattacgcaa tattattggc ataccgtggc gtttaaattg gggataatat
     1021 ccatacacct aaatatcact ccatgaaagc tacccctgaa cgcatgtttc aggtgatcga
     1081 agtgagtagc agacggatca aggaaattcg ctcgatgtca tcttttttcg cgctgattgt
     1141 gcttatctgc gcgatgctga tagcaccggc agctgcagac tccctgccgg acagggtcta
     1201 tgtgaacgca tcgtataacg gtgaaacgcc cggttacggg acactgaact tctcgagcat
     1261 ccaggaggcc gtcgataatg tcacggatgg cggcgaggtg tgggtctaca acgggaccta
     1321 caacgagccg atcgtgatcg acagggccat gaccgtcacg accgtctccc accttgaatg
     1381 ccccggcctc gacggcgtaa tcatcgacgc catgggtgag cagacaggca tcgagatcga
     1441 cgcagacgaa gtctccattt ccggctttga ggtcgtgaac gcctccttat tcgggatcta
     1501 cgcccacaac gccggggcgg tcaccatcga gtacaactcg gtcacggtcg agaacgacac
     1561 ctacgaactc acctgcggta tcctcatcga gggcggcgag ggagtctgca tcggcaacaa
     1621 cgcggtgcgt gtcatcggga gcagcgctca ggcgggcatc atggtcaatg gcgtcaccga
     1681 agccaacgtc cacgacaacc acgtcgtggc ggtctcgcgg caccagggtc tcgaagccgt
     1741 cgagatggaa cctaacccgg ttgttgccgc cctcttcacg gaggataatg aaccgttcat
     1801 cgctgccagg gcgaccgtca cacagcaggc gtctgccggg gtcatcatcg aagagagttc
     1861 ctctgttctc gttgagcgca acaggatcgt cgccgtgggt aactgcaccg aggacgctct
     1921 ggatctccac tactcggccg tcgaggcctg gggtatcggg tccggcggca actcgaagaa
     1981 cctgaagatc ctggagaacg acgtcacggt cgtcggaaag gctctcgaca cagcctggac
     2041 ggtcggtatc ctcggggtcg gagacctcgc gctcgtccag agcaacacgg tcgaggttat
     2101 cgccaggtcc cgggtcgtcc tgccggtagg catcgtgatc tccgaggccg agaaggggca
     2161 gatcttcgat aacaagatcg aggtagaggc cgcctgcatc ggcaaatccg acggagaagt
     2221 cagtccggag gccaccggca tcatagccgt cgagtccccg gaagcccagg ttctcaggaa
     2281 cgacgttctg tacacgctca ccctcaccga cgcaggccag ctggccatga cgtcgacaga
     2341 gggtatttac gttggcggat gcgacgagtc ccgggtgagc gagaacatcg tcctcatcca
     2401 gaccgggatc ttcaacctgc cggtcgacgg tgacgaatcc accgaagacg aagagtccct
     2461 gaccctgggg gtcgcgaggg tcaaaggcat cggcgtcgaa gcgagcgaca aacccgagat
     2521 ctgcgacaac gtcgtgcacg ccttcggcgc tgtcatgacc tacgatgaga ctgccgggga
     2581 tctcaatgtg ggcgccctct ccgctctcgt catgacaggc atccacgtct ggagctactc
     2641 cgacgacctc ctcgaaaacc ccgtggtccg cgagaacgtg gtcgtggtca gggcgcaggt
     2701 gcagaccaat ctggttcccg aactggaact cgatgaagaa gaggtaaaag ccgcccttgc
     2761 aggcaacagc gccctgctcg cccgttacgt cggcatgatg caggagagcg aggagttctc
     2821 cgtcgcaacg tgcctcgcgg acaccgaagg cctgtcggcc ctcacggaga gcgaccttga
     2881 gttcgaaccg gatatcgatg aggcgaccct cgctgccgca ctcacggtaa cagccggcat
     2941 cgtcctcgaa gatgtcctga acccggaggt ctgctgcaac tacgtgccgg tgtaccagga
     3001 cgtcgccgtc attgtgtttg accggccggt cgatgacgtt ggcatggtgc tccagactcc
     3061 tctctcgtta gggcttatcg accaggcctt ctcggatgag caagtgcagc aggcgttcct
     3121 gctcggcctc atcgacgagg acgagagcgc atggcagaac ctctctaaag acgacagagc
     3181 ggcgatcaca caggccgtcc ttgccggaga taccggcgtc cttgcagcct acaccgatga
     3241 cgagacgctc gccgggtatc aggcactcat ggaagacggg gaagcgttcg agttcctgaa
     3301 cacaacccgg cttgcggccg cgatcccgct ctcggtgagc tgcggcgtct acgtcaacga
     3361 cgccgaggga gacgtggtgc tccacgacaa cgagatcgtg gtagagacca acgccgccgg
     3421 cctcgcagcc gcgcaaggcg gcgacctcga tgcgcccgac gcggcggcgg gctccgccgg
     3481 cattgtagcg gcgttcggct tcgccggaac aggtgattcc atcagcgtcg aagagaactg
     3541 catccatgtg atcaccaacg gcatgttcct gggccaggcc atggggggtg aaaacgagac
     3601 cgccgataca gcagcaggca ccgcaaacct cttcctcgga gtgggagttc ttgccggtgc
     3661 agacggcgag cacgtcgtga acaacgacat tactgtcgca cagagaagcc gggcccagac
     3721 cctggcgctc aatatagtta aggataggac ggccctcagc gtcgcggcta cggcgggcgt
     3781 gggcatcggt atcgtcctcg atccatacga ggtcttcgat cccgagacct acgagcccgc
     3841 tccccctgcg ccggacgagt ggttcgcaag cgtcatcgag ggtaacacca ttgccgtcac
     3901 gaacgatatc aacatcttct cgctcgccga gatgctgctc cctgcacccc tcgacgggtc
     3961 gtcgaacgcg ctctccggcg cggcaggggc cgcggcgagc ttcggcatcg tcgcacccga
     4021 agccgacgtg ctcgataacg tcatcgagat acatgcggtt cagagcggtt acgcaggggc
     4081 ctttgtcgag gagaggccca tcattctagc gcagtcagcg gagatcgacc tgcccggcgc
     4141 cggggccgcg aacctgggag tcgccgtctc gaacggtatc ctgaccgtgc gctcctacat
     4201 cgagaacaac accgtctaca ccgcggcgaa ctccgaagct tccgttgagg catcgacgga
     4261 ggaactcctc gaagacgccg gcgttctcgc cggcgccgtg gcggccgata tcgggatcgt
     4321 gagcctgagc ccctcctaca tcgatgggaa caccgtggac ggagatctct ccatggccct
     4381 catcaccatg gtcaacggcg accgggtgga cggagacgtc ctcttcctcg gcctcgacct
     4441 cggcatcctc tcggtgggca gcgacgcgac gttcaacaac atccataacg gctacatggg
     4501 gttcccgtat tactacgatg atgaggagta cgagccctac gccgactaca actggtgggg
     4561 cgacgcgagc ggcccatcag gcctcggccc cggcacggga agcccggttg tcggcaccat
     4621 gaactacgag ccctggctga cccggccggc ggacgtcgtc cttgcgaccg gcaagtcata
     4681 cttcgggctc gagatagggt ctccgaacat cggcggcgac ggctaccgct ccggtctcga
     4741 accgggctgg accaccctct cgttcccgct tgcgctcgag aacagcacct ggcaggcggt
     4801 cacccacacc ggcggcggcc tcgactacag cgtcgcctgg acctgggacg ccgccggcca
     4861 gaaatgggtc cagatgaccg cgacgagcac gatcaacccg ctcgatgccg tctacatccg
     4921 tatgaacgat tacgaccgcc tcccggtcgc gatcagcccg gagatcacga gcccgccggt
     4981 gaagatgctc cgg
//
LOCUS       DAOSII010000127         4947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_15195_length_4947_cov_37.381235, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000127 DAOSII010000000
VERSION     DAOSII010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15195_length_4947_cov_37.381235"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..658)
                     /locus_tag="PLV90_08595"
     CDS             complement(101..658)
                     /locus_tag="PLV90_08595"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOF97213.1"
                     /translation="MGKSALAELFETEEKIRILRHVAKRNPVTATTVVAATGTSKALV
                     SRYLRLLVRNGFCMQRGREYAWRKNARSLAAKRLLNIDLLEAAVSFPGWARGIGVYGS
                     YAGGTNTAGSDLDLWMLADEYTSDLEVPATGVGKSAGAAAGTEASLFILTRERLSNLR
                     ATDQPFYSDLVRSSITLGGESIENS"
     gene            complement(794..1480)
                     /locus_tag="PLV90_08600"
     CDS             complement(794..1480)
                     /locus_tag="PLV90_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOF97214.1"
                     /translation="MYEDSYTNRLRKTAVDMGADLFGVADVQNFDDPAYTGNDPRGIM
                     EDARAVIVIGVAVPRGSIESLPKGRAEYTNTLMAGTATIRIIAFRLARLIEKEGYKAT
                     IVPTEGSEFGYWYADRETLMAGMSIKYAAYCAGLGNYGANHLLITDEFGPRVRMTAIL
                     TDAPLKGPEGPRRPLLNEDCKDCMKCVEACPAAAFTADGEIHRQRCAEYMFQALGGLR
                     CGLCIKVCPK"
     gene            complement(1818..2192)
                     /locus_tag="PLV90_08605"
     CDS             complement(1818..2192)
                     /locus_tag="PLV90_08605"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOF97215.1"
                     /translation="MKAPLLVACLCCALIVTLSSGGCTTNTKDQEYYDNWVAAEPDNA
                     EAWCIRGMYYNDNYGQYAEAMESCNRALELDPGYGLAWYLKGVILTNMNMTGEAAPCF
                     ENATRYDPGLAKSVQAVAGNTR"
     gene            2554..2638
                     /locus_tag="PLV90_08610"
     tRNA            2554..2638
                     /locus_tag="PLV90_08610"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2590..2592,aa:Leu,seq:gag)
     gene            2844..2990
                     /locus_tag="PLV90_08615"
     CDS             2844..2990
                     /locus_tag="PLV90_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97216.1"
                     /translation="MDDTAPRLGIPPIVYQDAINNHFDLLFLMLPDSHHSKISRCTPG
                     AHIR"
     gene            3154..3834
                     /locus_tag="PLV90_08620"
     CDS             3154..3834
                     /locus_tag="PLV90_08620"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HOF97217.1"
                     /translation="MRLGVLFSGGKDSLYACWKAMQHEEVACLITVVSKNPESYMFHT
                     PNIHLAGLQAAAAGLPLVEVETAGEQETELLDLKRALSLARERFDIEGVVTGAILSVY
                     QAARVQRICRELDLWCFNPLWHIDQEAYMEELIDAGFRVIVVGVFSSPFDASWLGREI
                     DRRTLDDLRKAARKYRITLTGEGGEFETFVIDAPFFSRRIAIEEASAVYQNYNGILRI
                     ERAGLVEK"
     gene            3831..4397
                     /locus_tag="PLV90_08625"
     CDS             3831..4397
                     /locus_tag="PLV90_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97218.1"
                     /translation="MILVIDLCYRGNSLSHAEFVGPVERYLREAGAEPIVRHYTTVSA
                     PDIEGADGAVLCGTALKDTGFLDHPDIFRWLSTFARPVLGISSGMLALAAAFGGGTVP
                     CCEIGMVDIRVVAPDPLFAGMTGFPAYELHEHAVETPTGFIPLVVSDRCIQAIRHRSL
                     PLYGLSFHPEVRNEWIIERFVPMVKRTH"
     gene            complement(4583..>4947)
                     /locus_tag="PLV90_08630"
     CDS             complement(4583..>4947)
                     /locus_tag="PLV90_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methanogenesis-associated radical SAM protein"
                     /protein_id="HOF97219.1"
                     /translation="VITGSIAAPFIQRVLSIRGYRSRVVRVRKEIACLITAEDLAGVN
                     LSKLEDVVIIPGRAFVHDVEARRILSADGVDREVVRGPDTLTADAETSMGMTRIEVLE
                     KEMEGFAALINTINQYGR"
BASE COUNT          917 a         1595 c         1443 g          992 t
ORIGIN      
        1 gataaatgcg agcgaggtgc gcttccgcac cctcgtgacc gccggttcgg agtctacaga
       61 aggtgcgacc ctccgaagct tcttctccct gaagcagtct tcaactgttc tcaatggact
      121 cacctccgag agtaatgctc gaccggacaa ggtcggagta gaacggttga tcggtcgctc
      181 tcaggttaga aagcctctcc cgagtgagga tgaacaggct tgcctcggtt cctgctgccg
      241 cacctgcact tttcccgact cctgttgcag gaacttcaag gtccgatgta tactcatcgg
      301 caagcatcca gaggtcaaga tcactgccgg ccgtgttcgt cccccctgca taacttccat
      361 agactcctat cccccgtgcc caccccggga atgaaaccgc cgcctcaagg agatcgatgt
      421 tgagcagacg tttcgccgcc agacttcggg cgttcttacg ccaggcgtac tcccggccgc
      481 gctgcataca aaaaccgttc ctgacaagca ggcgcaggta gcgcgagacc agggcttttg
      541 aggtcccggt cgccgcgaca actgtcgttg ctgttaccgg atttcgtttg gcaacatgcc
      601 ggaggatgcg tattttctcc tcggtttcga agagttcggc cagtgccgat tttcccatac
      661 cgcttctgcc ccgcgaacag attgtttccg ctccggaaac agaatattcc tgatccggca
      721 acgcctgcgg atgagaaaga ttccccggga ccggcgcccg ggttcgctac cggccctgcc
      781 cggagttacc ctctcacttc gggcacacct tgatgcagag gccgcaccgg agcccaccga
      841 gcgcctggaa catatactcg gcgcaccgct gccggtgaat ctctccatcg gcagtgaacg
      901 ccgcagccgg acaggcctcg acgcacttca tgcagtcctt gcagtcttcg ttcaagagag
      961 gccgccgggg gccttccggc cccttgaggg gggcatccgt gaggatggcg gtcatgcgaa
     1021 cccgggggcc gaactcatcc gtgatcagga gatggttcgc cccgtagttc ccgagacccg
     1081 cgcagtatgc tgcgtacttg atggacatcc ccgccatgag cgtctcgcgg tctgcatacc
     1141 agtacccgaa ttcgctgccc tccgtaggaa cgatcgtggc tttgtagccc tccttctcga
     1201 tcaggcgcgc cagccggaaa gcaatgatcc ggatcgtcgc cgtgcccgcc atcagggtgt
     1261 tggtgtactc ggccctgccc ttcggcaggc tctcgatcga gccccgcggg accgccaccc
     1321 cgatgacgat caccgcgcgt gcatcctcca tgataccccg aggatcgttg cccgtgtatg
     1381 ccgggtcatc gaagttctgc acatcggcaa ccccgaagag atccgcaccc atatcgacgg
     1441 cggtcttgcg taaccggttg gtgtatgaat cctcgtacat tctggtattc acctgtggtc
     1501 tcaggtttgg cggccgtttc gtatatacgt gcggggcgcg cggccagacc tcggacgctc
     1561 tcacttcggt atattcccgt gtaccgcggg ccaaaaccgc tccgggcagg cgggaacagc
     1621 catcatggat gagagatccc cgatggctgc tcccgcatgc cctcgccggc tgcctctgcc
     1681 ggcttgagcc ccggtatacc catcatcccc tgtcctggat aggcggaggg ccggcctcgc
     1741 gcggccgccg gcacccttct caagcattca tcccgtatct cgtaacaatc gatatttaag
     1801 ggatattggg gtccatatca ccgcgtattg ccggcaacgg cctgtacgct ttttgccagg
     1861 ccgggatcat acctcgttgc attctcaaaa catggcgccg cttcaccggt catattcatg
     1921 ttcgtcagaa taaccccttt caggtaccag gccagcccgt atcccggatc aagttcaagc
     1981 gctctgttgc aactctccat cgcctcagcg tactgcccgt aattatcgtt gtagtacatc
     2041 ccccggatgc accacgcctc ggcgttgtcc ggctctgctg caacccaatt gtcgtagtat
     2101 tcctgatctt tagtgttggt cgtacagccg ccggaggaga gcgtgacaat caacgcacag
     2161 cagaggcatg cgaccagcaa cggagccttc atggtagcgt attgaacccg gcggttaatc
     2221 aaggccacgc caccactgaa gggcgggagc atccttcgcg ggtgagacac ggcagcccga
     2281 cggttacgct tgtatgaaga accggagcct tattccgcat agagagaggc tagatagaga
     2341 aaacagcctt tcccgaccgg ggtgtgaccg tataccctgc acgacatagt gcagggcctc
     2401 tttgatcagc gtcctccggg gcattgccgg ggccctacca cctctccgca caaaggtatt
     2461 tcatgtatga caacagatta agtaaggtaa gcggggcgcg aaagcgcaat cagtgcattc
     2521 tccatcccgc gttcactatg ttctctcccc cgggcgaggg ttgccaagcc aggtcaaagg
     2581 cgccaggttg agggcctggt ctcgtaggag ttcgtgagtt cgaatctcac ccctcgcatt
     2641 tttcaaacat tttataccag cgccatcccg gctgccggaa catagtcgtc gcgacgccgg
     2701 gcgcagatac ccccgcatcc agctcaggaa ggaagaagag atagtgtttc cggggcttac
     2761 gccagcgctt cgtgacgctg ctgacagcgc gcctgtagtt cgctccattc atcggggtgc
     2821 tcttcggccc actcgaggag agcatggacg acacggcccc gcgactcggg atccccccga
     2881 tagtttacca ggatgcaatc aacaaccact tcgacctgct cttcttgatg ctgcccgact
     2941 cccaccatag caagatctcc cgatgcaccc ccggtgctca catccggtga aggttcaaca
     3001 gcatataact ctgatccccc cggcggcggc ggccggtaac cgcgcgattg gtcgggacgg
     3061 catgcagaac ccggacttcc accgcccggg tgcttccggc gccgccgccg caggggttat
     3121 cgtgcccgaa agagaacggg tacgcgaaca gccatgagac tcggcgtgct cttctcgggc
     3181 gggaaagact ctctctatgc ctgctggaaa gcgatgcagc atgaggaggt ggcctgcctg
     3241 atcacggtcg tctccaaaaa ccccgagagt tatatgttcc ataccccgaa catccacctt
     3301 gccgggctgc aggccgcggc ggcggggctc cccctcgtgg aggtggagac ggcaggggag
     3361 caggagacag aactcctgga cctgaagcga gctctctccc tcgcccggga acggttcgat
     3421 atcgagggag tcgttacggg agcgatcctc tcggtctacc aggccgcacg ggtccagcga
     3481 atctgccgcg aactggacct ctggtgcttc aacccgctct ggcatatcga ccaggaggcc
     3541 tacatggagg agctcatcga tgccggattc cgggtgatcg tcgtgggcgt cttctcctcg
     3601 cccttcgatg catcctggct cgggagggag atcgaccgcc gcaccctcga cgacctccga
     3661 aaggccgccc ggaagtaccg gatcactctc accggcgaag gcggggagtt tgagacgttc
     3721 gtcatcgacg ccccgttctt ctcccggagg atagcgatcg aggaggcatc ggcggtctac
     3781 cagaactaca acggcatcct ccggatcgaa cgggcaggac tggtggagaa gtgatcctgg
     3841 ttatcgatct ctgctaccgc ggcaactcgc tctcccacgc cgagttcgtc gggccggtcg
     3901 agcgctacct ccgggaggcc ggggcagagc ccatcgtccg tcactataca accgtcagcg
     3961 caccggatat cgaaggtgcg gatggggcgg tcctctgcgg gacggcgctg aaggatacgg
     4021 gatttctgga tcacccggat atattccggt ggctctctac gttcgcacgc ccggtgctcg
     4081 gcatctcctc gggcatgctg gccctcgcgg cggcctttgg cggcggcacc gtgccgtgct
     4141 gcgagatcgg tatggtggat atccgggtgg tcgcgccgga tcccctcttt gccgggatga
     4201 caggcttccc tgcgtacgaa ctgcatgagc acgccgtgga gacccccacc ggattcatac
     4261 cccttgtcgt atcggaccgg tgcatccagg cgatccggca ccgctcactc cctctttacg
     4321 gcctttcctt ccacccggag gtcaggaacg agtggatcat cgagcggttc gtcccgatgg
     4381 tgaagaggac ccactgattc cccccatacg cacagtgggt gaatgcccgg ggacaggggg
     4441 ttacaccata cccggaccat gccggcgaag gggtgagggg gtgccgcgct cgttcaaggt
     4501 cgcctcacgc cggcggccgg acgagcgctc cggcaaggat cgcctcgatc ccgtgctatc
     4561 cggccccggc cggaacggtc tctcatcgac cgtactggtt gatcgtgttg atcagggctg
     4621 caaacccctc catctctttc tcgagcactt ctatccgggt catgcccatg ctcgtctctg
     4681 catcggccgt cagcgtgtcg gggccgcgca ccacctcgcg gtcgacgcca tcggcggaga
     4741 ggatccttct cgcctcgacg tcgtggacga acgcccggcc ggggatgatc acgacgtctt
     4801 cgagttttga taagttcacg ccggcgaggt cctcggccgt gatgaggcag gcgatctcct
     4861 ttctgaccct gacgacacgt gaacggtagc cacggatcga gaggacccgc tggatgaagg
     4921 gggcggcgat gctcccggtg atcaccg
//
LOCUS       DAOSII010000128         4917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_15314_length_4917_cov_42.766146, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000128 DAOSII010000000
VERSION     DAOSII010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15314_length_4917_cov_42.766146"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /gene="pth2"
                     /locus_tag="PLV90_08635"
     CDS             <1..339
                     /gene="pth2"
                     /locus_tag="PLV90_08635"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-tRNA hydrolase Pth2"
                     /protein_id="HOF97220.1"
                     /translation="WKQCLIVRADVRMSCGKKCAQAAHAAIGAYEKADKLVKKAWYDE
                     GQKKVVLKAANERQLFELKTIAERAGIPASIIQDAGMTEIPPGTVTALGLGPARTEDL
                     DRITGDLPLL"
     gene            336..1607
                     /gene="truD"
                     /locus_tag="PLV90_08640"
     CDS             336..1607
                     /gene="truD"
                     /locus_tag="PLV90_08640"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HOF97221.1"
                     /translation="MMPSPYPLEENLGMRYYASSTPGIGGRLRSSPADFVVEELPLPI
                     SDPDGPYIICRLTKTNWELQRAAREIAKQLAISHRRIAWAGNKDKNAVTTQFLSIYGV
                     TPEEVGRVHLKDISLEVVGRSQHPLVLGGLAGNRFDIIIRDCVPEELAQRVRAVTEVV
                     AAGVPNYYGLQRFGVVRPVTHIVGEKILKGDYEGAVAAYIGCAYPGEPGDVQRARSHF
                     AETRDAGAALAGLPIRMTYERSMANHLVANPGDYAGALRVLPPKLLSLLVSAFQSYLF
                     NCALSSRIDAGIPLFEPEVGDRLLFHDGREDIVTARNRQTALVHIRRGRCGIAIFIPG
                     AEPVAPGGRMDEIMQELMQNHGIDAKDFARASRFVETAFAGVARPIALSAGVEADVMD
                     ASVRLRFTLPPGHYATTVCREYMKADPYAMI"
     gene            complement(1645..2337)
                     /locus_tag="PLV90_08645"
     CDS             complement(1645..2337)
                     /locus_tag="PLV90_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HOF97222.1"
                     /translation="MADHERPHVLMMSEITVDGKLTLRRGASSKILMKYMAPETEILL
                     HRTRAECDAIMVGANTIRIDNSFLTVRLVEGKSPLRVIPSNRGDIPPDAHILGPDART
                     IIAVSGAAPAEKVARLRESGVDVVVVGEGQVDLPGLMRILKADYNVSRMMIEGGPTLN
                     WSMLNHRLVDEIRLIHLPFIVGGADTPSLVGGMHIETENDMIRLSHKRHYMCGSNLVT
                     EWDVLYERSART"
     gene            2464..3096
                     /locus_tag="PLV90_08650"
     CDS             2464..3096
                     /locus_tag="PLV90_08650"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOF97223.1"
                     /translation="MNITSLRPKFIKYSEPVASFFIRLGITPNQVSMLSVLFGLSCAL
                     AFSQRYFLAGSLLLVVSAVLDLVDGSVARRNHTESRFGAVFDWVADKYVDAAVILGVG
                     FSGIPIVSHLVSVPPIADFGVVGIALAGSLINTFIKPVTYAEIGYTERIAGKIEDPLE
                     GVGFFGRPETILALVLGGVTGYIWVAILLIAVCTNLSAIQRILYLYRQYS"
     assembly_gap    3146..3245
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3288..4118)
                     /gene="artA"
                     /locus_tag="PLV90_08655"
     CDS             complement(3288..4118)
                     /gene="artA"
                     /locus_tag="PLV90_08655"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase A"
                     /protein_id="HOF97224.1"
                     /translation="MKDYLVLASCICFLVFLVPGRFRKYFAIGGWAGIVGYLFFELPY
                     YFSINNFMYPTIALLSVPFLAITVQHLLRDDPRVMQLSTIAAVAFLIYAPFGFIPALG
                     DWLIAAVADQTSAALAASGHPVNFSAWNTMERNGFRTEIILACTGIQSIAIMLGVAWG
                     VPSTARQKLAGFLLVFPTIYILNIARNVFVITAYSEQWFPYLPAIAGNGEFGYESFFW
                     AHNVMAELGALIFLVALAYALFLILPELGTLADSLYRLYRSEVERVLRPKAPQSGLQP
                     "
     gene            4246..4560
                     /locus_tag="PLV90_08660"
     CDS             4246..4560
                     /locus_tag="PLV90_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor S"
                     /protein_id="HOF97225.1"
                     /translation="MMFCPQCKGLMISSGGQLKCKKCGYIREINDLDRLKKTDKRVTK
                     EIVIVDAEDTIATLPTATIKCPDCECTTAFWWLRQLRAADESEVRFFRCTACGKTWRE
                     YD"
BASE COUNT          898 a         1473 c         1543 g          903 t
ORIGIN      
        1 tggaagcagt gcctgatcgt gcgtgccgac gtcaggatga gctgcggcaa gaagtgtgcg
       61 caggccgccc acgcggccat aggggcctac gagaaggccg acaaactcgt gaagaaggcc
      121 tggtatgacg aggggcagaa gaaggtcgtg ctgaaggctg caaacgagcg gcagctcttc
      181 gagctcaaga cgatagcaga gcgggcgggc atccctgcat ccatcatcca ggacgccggt
      241 atgaccgaga taccgccggg gaccgtgacg gcgctcggtc tagggcctgc acgcaccgaa
      301 gacctcgacc ggataaccgg cgaccttccc ctgctatgat gccctcgccc taccccctgg
      361 aagagaacct cggtatgcgt tactacgcat cctcaacccc cggcatcggc ggccggctcc
      421 gctcaagccc ggcggacttc gtcgtcgagg aactcccgct cccgatcagc gaccccgacg
      481 gaccctacat catctgccgg ctcaccaaga cgaactggga actccagcgg gcggcaagag
      541 agatcgcaaa acagcttgcc atcagccacc ggcggatagc ctgggcgggg aacaaggaca
      601 agaacgcggt gaccacccag tttctatcga tctacggcgt tacgcccgaa gaagtcggac
      661 gggtgcacct caaggatata tcgcttgagg tcgtcgggcg gtcgcagcac ccgctcgtcc
      721 tcggggggct tgccgggaac cggttcgaca tcattatccg cgactgcgtg ccggaggaac
      781 ttgcacaacg ggtgcgggcc gtcacggagg tggtggctgc cggggtgccg aactactacg
      841 ggctgcagcg gttcggcgtc gtccggccgg taacccatat cgtcggcgag aagatactca
      901 aaggggatta cgagggtgcc gtggccgcgt acatcggttg tgcatacccg ggagagcccg
      961 gagatgtgca gcgggcgcga tcgcactttg ccgagacccg ggatgcgggg gcggcgctcg
     1021 ccggtcttcc catccggatg acgtatgagc ggtcgatggc aaaccacctc gtcgcgaacc
     1081 ccggcgacta cgccggcgcg ctccgggtgc tccctccaaa gctcctctcg cttctcgtga
     1141 gcgcgttcca gtcgtacctc ttcaactgcg cgctctcgtc ccgcatcgac gccggcatcc
     1201 cgctctttga acccgaggtc ggcgaccggc tgctcttcca tgacggccgg gaggatatcg
     1261 tcacggcccg gaaccggcag accgctctcg tccatatccg gcgcggccgg tgcgggattg
     1321 ccatattcat ccccggggcc gaaccggtgg cgccgggcgg ccggatggac gagatcatgc
     1381 aggagctgat gcagaaccac ggcatcgacg caaaagactt cgcgcgtgcc tcccggttcg
     1441 tggagacggc gtttgccggg gtcgcaaggc ccatcgccct ctctgccggc gttgaagcgg
     1501 atgtcatgga cgcaagcgtc cggctccggt ttacgcttcc tcccggccac tacgcgacga
     1561 cggtctgccg ggagtacatg aaggcggacc cctacgcgat gatctgacgg cgcaaaaaag
     1621 atctctcgta tggcgcgggc cccttcaggt ccgcgcgctc ctctcgtaga ggacgtccca
     1681 ctcggtgacg aggttgctcc cgcacatgta gtggcgtttg tgggagagcc ggatcatgtc
     1741 gttctcggtc tctatgtgca tgccgccgac gagggatggg gtatcggcgc cgccgacgat
     1801 gaacggcagg tggatcaggc gtatctcgtc cacgaggcgg tggtttagca tggaccagtt
     1861 cagcgtgggg ccgccctcga tcatcatccg cgagacgttg taatctgctt tcaggatccg
     1921 catcagcccc ggcaggtcga cctggccttc tcccaccacg acgacgtcga cgccgctctc
     1981 ccggaggcgg gcgaccttct ccgccggagc cgccccgctg acggcgatga tcgtccgggc
     2041 atccggcccg aggatgtgtg catccggcgg tatatccccc cggttgctcg ggatgacgcg
     2101 gagcgggctc tttccctcca ccagccgaac cgtcaggaaa gagttgtcga tcctgatggt
     2161 gtttgccccg accatgatgg cgtcgcactc cgcccgggtc cggtgaagga ggatctcggt
     2221 ctcgggcgcc atgtacttca tgaggatctt gctcgatgct ccgcgccgca gggtgagttt
     2281 tccgtcgacg gtgatctccg acatcatgag tacgtgcggc ctttcgtgat ctgccatgaa
     2341 atctccttct ccctgatcta tctggcgggg gcgtttaaga atatggtgat tcccgacagg
     2401 atacagcgct ccctgcccgg cgaagcaaag agttaaactg ttttcgggct gataaattat
     2461 gcgatgaata taacctcgct gcggccgaaa ttcatcaaat attcagagcc tgtcgcttcg
     2521 ttttttatac gcctgggcat tacgcctaac caggtctcga tgctctccgt gctcttcggc
     2581 ctctcgtgcg cactcgcttt tagccagcgg tattttctcg cgggcagcct gttgctggtt
     2641 gtctcggcgg tcctcgacct ggtggacggg agcgtggccc ggaggaacca cacggagagc
     2701 aggttcggcg ccgtcttcga ctgggttgcg gacaaatacg tcgatgcggc ggtcatcctc
     2761 ggcgtggggt tctccggcat ccccattgtc agccacctcg taagcgtccc gccgatcgcc
     2821 gactttggtg tcgtcggcat cgcgctcgcc gggtcgctga tcaacacctt catcaaaccg
     2881 gtcacctacg cggagatcgg ttacaccgag aggatcgcag gaaagatcga ggaccccctc
     2941 gagggggtcg gtttcttcgg caggcccgag actatcctcg cgctggttct tggcggcgtg
     3001 acgggataca tatgggtcgc gatcctcctg atagcggtct gcacgaacct ctccgcgatc
     3061 cagcggatcc tctacctcta ccggcagtac tcctgaaggg aggctgccgc ctcttcatac
     3121 ggatgctatg ccgcgtcata cctgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnccggc gccggatcct cctcccgcat ccccggcgcc ggcgccctta tggttgcagg
     3301 ccggactgcg gggccttcgg ccggaggacc cgttccacct cgctgcggta gagccggtag
     3361 aggctgtcgg cgagggtgcc gagttcggga aggatcagga agagagcgta ggcgagggcc
     3421 acgaggaaga tgagcgcccc gagttcggcc atgacgttat gcgcccagaa gaaactctcg
     3481 tacccgaact cgccgttgcc ggcgatcgcg gggaggtagg ggaaccactg ctcgctgtat
     3541 gccgtgatga cgaagacgtt gcgcgctatg ttcaggatat agatcgtcgg gaagaccagg
     3601 agaaacccgg cgagtttctg ccgcgccgtc gacggcacgc cccaggcgac cccgagcatg
     3661 atcgcgatgc tctggatgcc ggtgcaggcg agtatgatct cggtccggaa accgttgcgc
     3721 tccatcgtgt tccaggcgct gaagttcacg gggtgcccgg atgccgcgag ggcggcggat
     3781 gtctggtcgg cgactgcggc tatcagccag tcgccgagag cgggtatgaa gccgaagggg
     3841 gcgtatatca ggaacgccac ggctgctatc gtcgagagct gcatcacccg gggatcgtcg
     3901 cggagcaggt gctgtacggt gatggcgagg aaggggaccg agaggagggc gatcgtcggg
     3961 tacatgaagt tgttgatgga gaagtagtag gggagctcga agaagaggta tccgacgatg
     4021 cccgcccatc cgccgatggc gaagtacttc cggaaccgtc ccgggacgag gaagacgaga
     4081 aagcagatgc aggaggcgag caccaggtaa tccttcatcc tttacctttc ggcgtatccg
     4141 gcaataaatc atttgtgcgc cggatctggc ctgcgccggt gtccgtgcgg ccccggttgt
     4201 ccggccgacc aaggattaat ttcagtcggt gccccacata taatgatgat gttctgtcca
     4261 cagtgcaaag gcctgatgat atcgtccggt ggtcagttga agtgcaaaaa atgcggatat
     4321 atccgggaga tcaacgacct cgaccggttg aagaagacag acaaacgcgt gacaaaagag
     4381 atcgtcatcg tcgatgcaga ggatacgata gcaaccctcc cgaccgcgac catcaagtgc
     4441 cccgactgcg agtgcacgac ggcgttctgg tggctccggc agctgcgggc ggcagatgag
     4501 agcgaggtca ggttcttccg ctgcaccgcc tgcgggaaga cgtggcgcga gtacgattaa
     4561 gtagaatctg ttgccctccg ggccctcttc ttcttccggc ggtttttgag agtgccggat
     4621 atcgttcctc gcgaatgcag gaggtatagg ccggtaactg agcctccggg gcaattgcaa
     4681 aaaattacgc gatgcctcgt ggggccgcag gcatgccgga tgaaaattgc gaagggtatc
     4741 ttcatacgaa aacgcggggt cagatcgcct tctctcactc cccccggcac cgggatcacc
     4801 ctcccccgga cgccggcata cggcgtgtcg ttacaggtgg cgacgacctg gcaatgggtc
     4861 agccgggccg gcctctctcc cattccgccc tcatcagaag atcccgccga gcaaacc
//
LOCUS       DAOSII010000129         4850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_15575_length_4850_cov_48.020438, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000129 DAOSII010000000
VERSION     DAOSII010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15575_length_4850_cov_48.020438"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..925)
                     /locus_tag="PLV90_08665"
     CDS             complement(161..925)
                     /locus_tag="PLV90_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOF97226.1"
                     /translation="MDEQMPLGVTITEIREETPSIRTFVFDRAIDARPGQFVMVWVPG
                     VDEIPMALSSPSSITVLKVGDATAALFAMQVGDRIGIRGPYGNGFSVTGRTLAVAGGV
                     GASPLLPLIKAGQVDTFLLGARTASELLFADQIRGAATLMVATDDGTAGHHGFVTRLM
                     SRVDLADFAHICVCGPEVMMAAVLAILDREGCADRGQFSLHRYMKCGVGLCGSCCTDP
                     HGLRVCRDGPIFSGDVLLESDFGRYTRDASGRRQRI"
     gene            complement(918..1805)
                     /locus_tag="PLV90_08670"
     CDS             complement(918..1805)
                     /locus_tag="PLV90_08670"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOF97227.1"
                     /translation="MVALYPGPVDIGGVRLKNHLLLAAGILGTTGASLARILANGAGG
                     VVTKSIGPAPKEGHPGPCLIVVDGGIINAMGLPNPSAAFADELIALTGEPVVVSIFGG
                     TPEEFRTVAGWFAGKAAGLELNLSCPHAEGYGAAIGSDPALVEECTRAAASPGVPTWV
                     KLTPNVADIGEIGAAAERGGADAVVAINTVKAMRISTSLGRPVLGNRFGGLSGKAIFP
                     VAVRCVYDLYEACSIPIIGCGGISTADNVVEMMMAGASAVEIGSAVIDDVGIFGTIAR
                     DLYSHDGIAARDIVGCAHG"
     gene            2010..2876
                     /locus_tag="PLV90_08675"
     CDS             2010..2876
                     /locus_tag="PLV90_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HOF97228.1"
                     /translation="MTSLAISGVGKVGGETAFLSAALGLADEIILYDAYEPILRSQVL
                     DLQHTGIDVTVTTDTAAMRDADVFIFAAGTPRTPDIKTRADLLEANIPVVRRCSEHLR
                     DFPGVVITITNPMDANNYALWKMMGIDRERCIGFGGQLDSVRFACLLREAGIPGPAWV
                     LGEHGEHQVPLFSKTGRYVDTGERETILSRMRGASMEVIRGKGATVFGPAYHIAMLVR
                     AVLEDRRELLSCSCVLDGEYGFSGCSLAVPARIGREGILGIEEWDLDPWEAAKMTEAG
                     AFVRGLCRRFDD"
     gene            2869..>4850
                     /locus_tag="PLV90_08680"
     CDS             2869..>4850
                     /locus_tag="PLV90_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase"
                     /protein_id="HOF97229.1"
                     /translation="MTDPAPESRVTIPATLNDFGKIRIGIHQVEYSVGVDIPVIHIFG
                     RDASGKAVQVRVTGFRPYFYVPAGLVNDPSPPKGVDIEPGTTYRSIRGESLRRLYTRR
                     PGDVRAVRDDFRQHYEADIPFTTRFMIDCGLTAGVELPAGAVESPEGTFEVEYTDIAP
                     ADIKAPARTCIMDIECVDEHGFPEPERDPIVCVTCWDSVEEDYTTLLWQPGGAAGDAP
                     ELSTNARHKVTRYPNEAAMLRGLAAYVRERDPDILSGWNFVDFDIPYILQRMARLNLP
                     GEDLARLPGQTERNAVRGRSIFDLLGAYRKMHQAQKESYRLDAIAGEELGETKVRYSG
                     TITDLWRSDPARLVEYNCRDVELCVGIDRKNNIIEFYREISRYVGCPLDRTLNSSNVI
                     DIYVLRKASGRFVLPSKGLAAGDEFEGATVFEPATGLRENVVVLDLKSLYPMAMMTIN
                     ASPETKNPDGELRAPNGIRFSREPDGLTRSILSELLVERDERKRLRNQYPFGSPEYVL
                     YDLQQNVLKVIMNSYYGVSGYTRFRLYDREIGSAVTSVGRAIIRHTRDVITDLGYTVL
                     YGDTDSCMVEVPQGSLEETIAKAKAIEARLNASYSDFAKSELNADTHYFSIKFEKVYR
                     RFFQAGKKKRYAGHLVWKEGKDVDEVDVVGFEIRRS"
BASE COUNT          890 a         1631 c         1524 g          805 t
ORIGIN      
        1 gcattctcct ggaccttcga ggccgactct tccttcttcg cctgcagttc ctcgagggtc
       61 tcaaggagcc gggtgtatcc ttcttcgatg atcggatcca ttgttcacct aactcttgcg
      121 tatgccggca taagcgcacc ggtttgtcgt ccggcggggc ctaaatcctc tgccgccgcc
      181 cgctcgcgtc gcgcgtgtag cgcccgaaat cgctctcgag caggacgtcg ccggagaaga
      241 tcggcccgtc cctgcagacc cggaggccgt gcgggtcggt gcagcacgaa ccgcagaggc
      301 cgaccccgca cttcatgtag cggtggaggg agaactggcc ccggtccgca cacccctccc
      361 ggtcgagtat ggcaagcacc gccgccatca tgacctcggg cccgcagacg cagatgtggg
      421 cgaagtcggc gaggtccacc cttgacatga gccgggtcac gaacccgtgg tgaccggccg
      481 tgccgtcgtc ggtcgcgacc atcagcgtcg ccgcaccccg tatttggtct gcaaagagga
      541 gttctgaggc cgtgcgggcg ccgaggagga aggtgtccac ctgcccggcc ttgataagcg
      601 gcagcagcgg ggaggcgccg acgccgccgg cgacggcaag cgtcctcccg gtgacggaga
      661 acccgttccc gtaaggaccc cggataccga tccggtcgcc tacctgcatg gcaaagaggg
      721 cggcggtcgc gtcgcccact ttgaggacgg tgatcgagga gggcgaggag agggccatcg
      781 gtatctcgtc gacgcccggc acccagacca tgacgaactg gcccggccgg gcgtcgatag
      841 cgcggtcgaa gacaaacgtc ctgatcgaag gcgtctcctc tctgatctcc gttatcgtga
      901 ccccgagggg catctgctca tccatgggcg caccccacga tatcgcgtgc ggcgatgccg
      961 tcatggctgt agaggtcccg cgcaatcgtg ccgaagatgc cgacatcgtc gatgacggcg
     1021 ctccctatct cgacggcgct cgcgcccgcc atcatcatct cgacgacgtt gtcggcggtg
     1081 gatatgccgc cgcacccgat gatcgggatg gagcaggcct cgtagagatc gtagacgcac
     1141 cggacggcga ccggaaagat cgccttcccc gagaggccgc cgaagcggtt cccgagcacc
     1201 ggccggccaa gggacgtcga tatccgcatc gccttcaccg tgttgatagc gacgacggcg
     1261 tccgcacccc cgcgttccgc ggccgccccg atctcgccga tatcggcgac gttcggcgtg
     1321 agtttcaccc aggtcgggac cccaggagag gctgccgccc tcgtgcactc ctccacgaga
     1381 gccggatcgc tcccgatcgc cgccccgtag ccctccgcgt gggggcagga gaggttcagc
     1441 tcaagcccgg cggccttacc ggcaaaccac ccggcgaccg tccggaactc ctccggcgtc
     1501 cccccgaata tgctcacgac gaccggttcg cccgtgagag cgatcagctc gtccgcgaac
     1561 gcggccgagg ggttcggcag tcccatggca ttgatgatgc cgccgtcgac gacgatcagg
     1621 cagggtccgg ggtgcccctc cttcggggcg gggccgatgg atttcgtgac cacgccgcct
     1681 gcaccgtttg caaggatacg ggcaagcgac gccccggtgg tcccgaggat gccggccgca
     1741 agcaggagat ggttcttcaa ccggacgccc ccgatatcga cagggcctgg atacaacgca
     1801 accattggga aaaggtggga gtgaggtatt attataccct cgcgccaagt ataatgaaga
     1861 ggaagtatgt gctccgcgcg ccggggcccg gataccggac cgaaagacgg cagggtgccc
     1921 tttctccccg ccgaaccgct atcctccgtg cagcagggcc ttatcacgca tacaatcttt
     1981 tgccgttatc cagacacagg acagatacca tgacctcgct tgcaatatcc ggtgtaggaa
     2041 aggtgggggg tgagacggca tttctctccg ccgcactcgg tctcgccgat gagatcatcc
     2101 tctacgacgc atacgaaccc atcctccggt cccaggtgct cgacctgcaa cacacgggta
     2161 tcgacgttac ggtcaccacc gatacggcgg cgatgcggga tgccgacgtc ttcatatttg
     2221 cggcaggcac accgagaacg cccgatatca agacccgggc ggatctcctc gaggcaaaca
     2281 tccccgtggt gaggcggtgc agcgagcatc tccgggattt tcccggggtc gtcatcacca
     2341 tcacgaaccc catggatgca aacaactacg ccctctggaa gatgatgggg atcgaccggg
     2401 agcgctgcat cgggtttggc ggccagctcg acagcgtccg atttgcctgc ctcctccgcg
     2461 aggcgggtat accggggccc gcctgggtgc tcggcgagca cggcgagcac caggtgccgc
     2521 tcttctctaa gaccggcagg tacgtcgata ccggggagcg ggagacaatc ctctcccgga
     2581 tgcggggagc aagcatggag gttatccgtg gaaagggcgc cacggtcttt ggccccgcat
     2641 accatatcgc catgctggtg agggcagtcc ttgaggaccg gcgcgagctc ctctcctgct
     2701 cgtgcgtgct cgacggcgag tacggcttct ccggctgctc gcttgccgtc ccggcccgga
     2761 tcggccgcga gggtatcctc ggcatcgagg agtgggacct cgacccgtgg gaggcggcga
     2821 agatgacgga ggcgggagca ttcgtgcgag gcctctgcag gaggtttgat gactgatccg
     2881 gcaccggagt cccgtgtcac tatcccggct accctgaacg atttcggcaa gatccggatc
     2941 ggcatccacc aggtggagta cagcgtcggc gtcgacatac cggtcatcca catattcggc
     3001 cgcgacgcct ccgggaaggc cgtccaggtc agggtgaccg gttttcggcc ctacttctac
     3061 gtgcccgccg gactggtgaa cgatccttcc cccccaaagg gggtcgatat cgagcccggc
     3121 acgacctacc gctccatccg cggcgagtcc ctccgccggc tctacacccg gcgccccggc
     3181 gacgtccgtg ccgtccgcga cgacttccgg caacactacg aggccgatat accgttcacc
     3241 acccggttca tgatcgactg cggcctcacc gccggcgtcg agctgcccgc cggcgccgtc
     3301 gagtcgcccg agggcacctt cgaggtcgag tataccgata tcgcgccggc cgatatcaag
     3361 gctcccgccc gcacctgcat catggatatc gagtgcgtgg atgaacacgg gttccccgaa
     3421 cccgagcgcg acccgatcgt ctgcgtcacc tgctgggact cggttgagga ggactatacg
     3481 acgctcctct ggcagcccgg cggggctgcg ggcgacgcgc ccgaactctc caccaacgcc
     3541 cggcacaagg tcacccgcta cccaaacgag gccgcgatgc tccgggggct cgccgcatac
     3601 gtcagggagc gcgaccccga catcctctcg ggctggaact tcgtggattt cgatatcccc
     3661 tacatcctgc agcggatggc aaggctcaac ctcccgggag aggaccttgc gcgtctcccc
     3721 ggccagacgg agaggaacgc cgtccgcggg agatcgatat tcgacctcct cggcgcctac
     3781 cggaagatgc accaggccca gaaggagtcc taccggctcg acgcgattgc cggcgaggaa
     3841 ctcggggaga cgaaggtccg ctacagcggc acgatcaccg acctctggcg atccgaccct
     3901 gcgcggctcg tggagtacaa ctgccgcgac gtcgagctct gcgtcgggat cgatagaaag
     3961 aacaacatca tcgagttcta ccgcgagatc tcccggtacg tcgggtgccc gctcgaccgg
     4021 acgctgaact cctcaaacgt catcgacata tacgtcctcc ggaaggcgtc gggccggttc
     4081 gtcctcccct caaagggcct tgccgccggc gacgagttcg agggggcgac cgtctttgag
     4141 cccgcgacgg gccttcggga gaacgtggtg gtcctcgact taaagtccct ctacccgatg
     4201 gcgatgatga cgatcaacgc atcgcccgag accaaaaacc ccgacggcga actgcgggcc
     4261 ccaaacggca tccggttctc ccgggagccg gacgggttga cgcgcagcat cctctcggag
     4321 ctcctcgtgg agcgggacga gcgcaagcgc ctccgcaacc agtatccctt cgggtcgccg
     4381 gagtacgtcc tctacgacct ccagcagaac gtcctgaagg tgatcatgaa ctcctactac
     4441 ggcgtatcgg gctacacccg gttcaggctc tacgaccgcg agatcggttc ggccgtcacg
     4501 tcggtcgggc gggcgatcat ccggcatacc cgcgacgtca tcaccgatct cggctacacg
     4561 gttctctacg gcgacaccga ctcctgtatg gtcgaggtgc cgcaggggag ccttgaggag
     4621 acgatagcca aggcaaaggc gatcgaggcc cgcctgaacg caagttacag cgactttgca
     4681 aagagcgagc tcaacgccga cacccactac ttctccatca agttcgagaa ggtctaccgg
     4741 cgcttcttcc aggccggcaa gaagaagcgc tacgccggcc acctggtctg gaaggagggc
     4801 aaagacgtcg acgaggtcga cgtcgtcgga ttcgagatcc ggcggagcga
//
LOCUS       DAOSII010000130         4825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_15659_length_4825_cov_47.493711, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000130 DAOSII010000000
VERSION     DAOSII010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15659_length_4825_cov_47.493711"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PLV90_08685"
     CDS             <1..281
                     /locus_tag="PLV90_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOF97230.1"
                     /translation="LMALLHDDFYSIRREAAAALVAVGPPAMGPVISALGDPDGDVRK
                     RAADVLAGIGDARAVEPLRSIERDEDWYVRKAAEEAVEKIRGRIGESV"
     gene            complement(303..1790)
                     /locus_tag="PLV90_08690"
     CDS             complement(303..1790)
                     /locus_tag="PLV90_08690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF97231.1"
                     /translation="MTPAISPGGRKTLPSPLAQGDGILIVDADQVVVQANIQVQALLC
                     ATREEILGADAPDVVQRYLLPLLPEGESPGEVVALLCDGSYPQPVTLDVRVPGTGIRR
                     ISVTANTVDAVTPALRLLVFHDTGKGGAGRAEERPPPATERLIVVPGRERRTPEQEVP
                     GSGDVTTAARRSVADTAVLEQIAEDEDRFRSIFATSHAVMLIVDPQTGAIVDANPAAS
                     AYYGHPLETLTTMQIADLNVLDREDLFRDMQKAVTGEKRQFSFLHRLADGRYRDVDVF
                     SGRVIICGRVLLHSIIHDVTGRRRAEEQFRALLDATPDAAMLIDHSGIILALNEVMAT
                     RFKKTVEELLGACAYDILTPELAMRRRELTIQALATGKPLRHVDEREGRILENVLFPV
                     PGDQEDSRRVAVISHDITRERELEQARQEAFDRIRQNIEQFAILGDHIRQPLQVILGT
                     ACLLEDEQATGKIQREIERINGYIRQLDQGWIDSRKIREYLQRHE"
     gene            complement(1897..2067)
                     /locus_tag="PLV90_08695"
     CDS             complement(1897..2067)
                     /locus_tag="PLV90_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97232.1"
                     /translation="MKRDRKGEGKAETEEYWCEICGAACDEITEETFPDLRKARTLCP
                     GCRKSYEDSCRL"
     gene            complement(2147..2971)
                     /locus_tag="PLV90_08700"
     CDS             complement(2147..2971)
                     /locus_tag="PLV90_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOF97233.1"
                     /translation="MAKKTILITGSTDGIGRAAAYLLAEQGHRILIHGRDRAKGRAVL
                     EEVEAAAVPEQPALFIADLSVQDRVRDLAEEVAGACDRLDVLINNAGVFMPERHVAPG
                     GIEMTFAVNYLAGLLLTHGLLPLLSKSAPARVVNVASIAHRSVRAVDWENIPAFEDYD
                     AYGAYATSKLGVAACTARLASVLDGTGVTANSLHPGVIDTKLLRAYTGGRDGGAPPEK
                     GAAVEAHLATSPDAGEVSGRYFEATRWTRPSPLALDADVQERFWEMGLDLVGVDRW"
     gene            3291..>4825
                     /locus_tag="PLV90_08705"
     CDS             3291..>4825
                     /locus_tag="PLV90_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOF97234.1"
                     /translation="MVRYSVTMDDDLVKTIDRMCDSLGMSRSEWLNKLCTAHLSGAPG
                     NGGGQGVAPTLMPPLQAPGPNMTDYETACASFKIDVPDYFNFGYDVIDRWAETDRNKL
                     AMIWVDQQGAEKKYSFRDLRNLSNGAANILLKYGVKKGDRVMLILPRIPEWWIFVIAL
                     IKLGAIFCPCPTMLTPKDIRYRIRAGKFRMIITNTENAEKIDEVADACPLLETLFLVD
                     GERDGWASYLHELDYPAPVSRHTVSMPVAKRTKSTDPMMIYFTSGTTGEPKMVLHNQG
                     YPLGHIITASLWQDLTPNDLHLTFSDTGWAKCAWGKIFGQWIAGACIFVYDIQGKFGA
                     TELLPLIEKYEVTTFCAPPTVYRMLILADLDKFDFRNLRHCCSAGEPLNPEVIRVWQD
                     GTGQPIYEGYGQTETVCCIATFPCMEHKPGSMGRPVPGWHIELHDDDGNPVGAGEEGK
                     IAVSLNPRPVGLFMEYLDNPEANCESFQNGFYYTGDKARMDEDGYLWFIGRSDDVIKS
                     SGYR"
BASE COUNT          834 a         1552 c         1509 g          930 t
ORIGIN      
        1 ccctcatggc cctcctccac gacgacttct acagcatccg gcgggaggcc gccgcagcgc
       61 ttgtcgcggt aggacctccg gccatggggc cggtcatatc cgcgctcggg gatcctgacg
      121 gcgatgtcag gaaacgggcg gcggatgtgc ttgccggtat cggggatgca cgggcggtcg
      181 agcccctcag gagcatcgag agggacgagg actggtatgt ccggaaggcg gcggaggagg
      241 cggtggagaa gatccgggga cgcattggag agagcgtgta gggattccgc tcgatcccgt
      301 agttactcgt gccgctggag gtactcccgg atcttcctcg agtcgatcca gccctggtcg
      361 agctgccgga tatacccgtt gatccgttcg atctcccgct ggatcttacc ggtcgcctgc
      421 tcgtcctcca gcaggcatgc cgtgccgagg atcacctgga ggggctggcg gatgtggtcg
      481 ccgaggatag cgaactgctc gatgttctgc ctgatccggt cgaatgcttc ctgccgggcc
      541 tgttcgagtt cccgctcgcg ggtgatgtcg tgggatataa ccgcaactct ccggctgtct
      601 tcctggtccc ccgggaccgg gaagaggacg ttttcgagga tccgcccctc ccgctcatcg
      661 acatgccgga gaggttttcc cgtggcgagg gcctgtatgg taagctctcg ccgccgcatc
      721 gccagttccg gcgtgaggat gtcgtaggcg catgccccga ggagctcctc gacggttttt
      781 ttgaaccgcg tcgccatcac ctcgttcagg gcgagtatga taccgctatg atcgatgagc
      841 atggccgcat cgggtgtggc gtcaagaagc gcccggaact gctcctctgc tcgccgccgc
      901 ccggtgacgt cgtggatgat ggagtgcagg agcacccgcc cgcagatgat cacgcggccg
      961 ctgaagacgt ccacgtcgcg gtatcgcccg tccgccagcc ggtgcaggaa cgagaactgc
     1021 cgcttttcgc cggtcaccgc cttctgcatg tcccggaaga gatcttcccg gtcaaggacg
     1081 ttgaggtcgg cgatctgcat cgtggtgagg gtctcgagcg gatggccgta gtaagcgctc
     1141 gccgcggggt tcgcatcgac gatggcaccg gtctgtgggt cgacgatcag catgaccgcg
     1201 tggctcgtgg caaaaatgct ccggaacctg tcttcgtcct ccgcgatctg ttccaggacg
     1261 gcggtatcgg cgacgctcct cctggctgcc gtggttacgt ctcccgagcc gggaacttcc
     1321 tgttccgggg tccgcctttc tctgccgggc acgacgataa ggcgctctgt cgccggggga
     1381 ggcctctcct ccgcacgccc ggcacctccc tttccggtgt cgtggaagac gaggagccgg
     1441 agcgcgggcg tgacggcatc gaccgtgttt gccgtgacgg atatcctccg gataccggtc
     1501 ccgggaaccc ggacatcgag cgtgaccggc tgaggatacg atccgtcgca gaggagggcg
     1561 acgacctccc cgggagactc gccctccgga agcagcggca aaagatatct ctgcacgaca
     1621 tcgggagcat ccgctccgag gatctcttcc cgtgtcgcgc aaaggagggc ctgcacctgt
     1681 atgttagcct gaacaaccac ctggtcggcg tcgacgatga ggatcccgtc gccctgcgca
     1741 aggggagatg gcagggtctt acgcccaccc ggggatatcg ccggagtcat ggatgagatg
     1801 gttctctggt tcggttgaag gatctctttc gccggagaat aagtactttt accatccgtc
     1861 tcgtccgggc atgtacccgt gcctcccgcc ccatcactac agccgacagg agtcctcgta
     1921 ggacttcctg caccccggac agagcgtccg ggcctttctc aggtcgggga atgtctcttc
     1981 ggtgatctca tcgcaggccg ctccgcatat ctcgcaccag tactcttccg tctcagcttt
     2041 cccctcgcct tttctgtccc gtttcatgca acccctctcc gtgcccggtt gtccggcccc
     2101 gggcgggttc ctgctctcct ccatccggca tatgctttcc cccggatcac catcgatcca
     2161 ccccgacaag atcgagcccc atctcccaga accgttcctg cacgtccgca tcgagagcaa
     2221 gcggcgacgg ccgggtccac cgggtcgcct cgaagtacct gccgctcact tctcccgcat
     2281 ccggcgatgt ggcaaggtgc gcctcgaccg ccgccccctt ctcgggtggt gcaccaccgt
     2341 cccgcccgcc ggtgtaggcc cggaggagtt tggtatcgat cacccccggg tggagggagt
     2401 tcgccgtcac ccccgtcccg tcgaggacgg aggcgagccg ggcggtgcag gcggcgactc
     2461 cgagttttga cgttgcataa gcaccgtagg cgtcgtaatc ctcgaaggcc ggaatattct
     2521 cccagtcgac cgcccgaacg ctccggtggg cgatcgatgc gacgttgacg acccgcgccg
     2581 gtgcgctctt cgacaggagc gggaggaggc cgtgggtgag caggagccct gcaaggtagt
     2641 tcacggcaaa cgtcatctcg atcccgccgg gtgcaacgtg ccgctccggc atgaagaccc
     2701 cggcattgtt gatgagcacg tcgagccggt cgcatgcccc ggcgacctcc tctgcaaggt
     2761 cccggacccg gtcctgcacc gagagatcgg cgatgaagag ggccggttgc tccgggacgg
     2821 cggctgcctc cacctcctca aggacggcgc gccccttcgc ccggtccctg ccgtggatca
     2881 ggatgcggtg gccctgttcg gccaggagat atgctgctgc cctcccgata ccgtccgtcg
     2941 agcccgtgat caggatggtc ttcttcgcca tacgcggcca gggtgcgcac cgtaattatt
     3001 tgaacgttgt gtgccggggc ggcaatccgg tgcaccctcc aggcaggggc ggcagcggtg
     3061 accggacacg aggtccgtac cctgcaccac ccctcccttt ccgtggcggg tggaggaccg
     3121 gcacggtctt tccggttctg gccggggtgc gcccctcccc tcggggccgg caccgttcac
     3181 ctctcccccg cccggcttcg ggtctttctg caccgctgag aaccccggtc atcgatttct
     3241 ggtgcgagat gaatcaaaaa ggtgatatgt tgcaccacag aactgtacgt atggtgcgat
     3301 actctgtcac gatggacgat gaccttgtga agacgatcga caggatgtgc gactctctcg
     3361 ggatgtcccg ctccgaatgg ctcaataaac tctgtacggc ccacctgagt ggtgcgccgg
     3421 gtaacggtgg cgggcagggc gtggccccga cattgatgcc gcctctgcaa gcccccggtc
     3481 ccaacatgac cgattacgag accgcctgcg ccagtttcaa gatcgacgtc cccgattatt
     3541 tcaacttcgg atatgacgtg atcgaccggt gggcagagac cgatcggaac aaactggcaa
     3601 tgatctgggt ggaccagcag ggggccgaga agaagtactc cttccgcgac ctccggaacc
     3661 tctccaacgg cgccgcgaac attctcctga agtatggcgt caagaagggt gaccgggtca
     3721 tgctgatact cccgcggata ccggaatggt ggatctttgt tatcgcgctc atcaagctcg
     3781 gagcgatctt ctgcccatgc ccgacgatgc tgacccctaa agatatcaga taccgtatac
     3841 gggcggggaa gttcaggatg atcatcacca acaccgagaa cgccgagaag atcgatgaag
     3901 tcgctgatgc ctgcccgctg ctcgaaaccc tcttcctcgt ggatggggag cgggacggtt
     3961 gggcgagtta cctgcacgag ctcgattacc ctgcaccggt atcgcgccac accgtcagca
     4021 tgccggtcgc aaagaggacg aagtcgaccg acccgatgat gatctacttc acctcgggca
     4081 ccaccggtga gccgaagatg gtgctgcaca accagggcta cccgctcggg cacatcataa
     4141 ccgcctcgct ctggcaggat ctcaccccaa atgaccttca cctgacgttc tcggataccg
     4201 ggtgggcgaa gtgtgcctgg ggcaagatct tcggtcagtg gatcgccggc gcctgcatct
     4261 ttgtctatga tatccagggt aagttcggcg cgaccgagct ccttccgctg atagagaagt
     4321 acgaggtgac cacgttctgt gcgccgccga cggtctaccg gatgctgatc cttgccgatc
     4381 tcgataagtt cgacttccgg aacctccggc actgctgcag cgccggcgaa ccgctcaacc
     4441 ccgaggtgat ccgcgtctgg caggacggca ccgggcagcc catctatgag gggtacggcc
     4501 agacggagac cgtctgctgc atcgccacgt tcccctgcat ggagcataaa cccggttcga
     4561 tgggtagacc cgtcccgggc tggcatatcg agctccacga cgatgatggg aacccggtcg
     4621 gtgccgggga ggaggggaag attgctgtca gcctcaatcc ccggccggtc ggactcttca
     4681 tggagtacct tgacaacccc gaagcgaact gcgagtcgtt ccagaacggg ttctactaca
     4741 ccggcgacaa ggcacgtatg gacgaggatg gctacctctg gttcatcggg cgcagcgacg
     4801 acgtcatcaa gtcgtccggc taccg
//
LOCUS       DAOSII010000131         4805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_15748_length_4805_cov_47.445053, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000131 DAOSII010000000
VERSION     DAOSII010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15748_length_4805_cov_47.445053"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..72)
                     /locus_tag="PLV90_08710"
     tRNA            complement(1..72)
                     /locus_tag="PLV90_08710"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37..39),aa:Arg,seq:ccg)
     gene            complement(107..1156)
                     /gene="hypD"
                     /locus_tag="PLV90_08715"
     CDS             complement(107..1156)
                     /gene="hypD"
                     /locus_tag="PLV90_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOF97235.1"
                     /translation="MAVGKDILTALFDLVDRDITLMHICGTHEAAIARAGLRSILPER
                     LKIVMGPGCPVCITPQGEIDAALDLVERGCTVATYGDLLRVPGSKGSLESSGGDVRVV
                     QGIHRAVEIAEREPDREVVFISVGFETTAPTVAATILSRPPENFSILSCHRLVPPAMA
                     WLLARGEAALEGFLLPGHVCAVMGYKEYEQFPVPQVVAGFEPEDILLGLLMAVRQVRE
                     GAHRVENAYPRAVSREGNVKAQRLMYEAFEPFDVEWRGFPVIPASGLRLKPEFEGYDA
                     QKKFDIEIRHVDKHSACICDKVLRGVARPSDCRLFAKACTPRTPIGPCMVSHEGACKI
                     WHLYESRRASNPPSR"
     gene            complement(1220..2152)
                     /locus_tag="PLV90_08720"
     CDS             complement(1220..2152)
                     /locus_tag="PLV90_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOF97236.1"
                     /translation="MAYRYLFGPVPSRRLGRSLGIDLVPQKTCTFDCAYCECGRTTVL
                     TLERREYVPTDRVIAELDDCLAKAPDIDYVTFAGSGEPTLHSGIGDIISFIKDRYPRY
                     RVAVLTNGALFVDPDVRAALMRADLVVPSLDAVSEDIFRDINRPSPGITAGQVVAGLV
                     AFSREFPGVIWLEVFIVPGKNDTDEELLRLRDAVAVIAPDRVQVNTLDRPGTDIRVRR
                     ASPRTLERARTIIGGNAEVIGAASCEPATAPEMRDVADMLLATLRRRPCTVADLSRIS
                     GLRPAEVTKHLRILEEKGAVEPVREQRGVFYRAT"
     gene            complement(2226..>3353)
                     /locus_tag="PLV90_08725"
     CDS             complement(2226..>3353)
                     /locus_tag="PLV90_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97237.1"
                     /translation="KAEIFSFIESNAEAIDENFPSFFGRVVTILDRAGVPIPDAAHDE
                     FIDAIAYRVFDVSKRAGDVKFVNRVCEIACGMKRKKERKAGVHLAAAVKLMKIGMPLT
                     AAAYLQPYRRHDAVVGCWHAYCYYTLYKEGSAEPGYSAAERWNYLKLARQHLDELGQL
                     RPGLWRLVRGELEQDRWLAEPFWMMIFLATEWMPDNRWFLEIGLSRAKQENNSVALVK
                     LLQIGLVRFPGDMLFYREAYNLKFDQGDLADALGLISDMLQRFPDDLEPIYYGLRTTL
                     YLPREGEFNGFRTLAEEKKMPGHVLCLIDYVYAFLRGRKEQANLCLDEFRRKYPSLSY
                     YSDLLRFATTADLPARPEGINQAIFSSVDQFCKRMLKIGDT"
     assembly_gap    3354..3453
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3454..3915
                     /locus_tag="PLV90_08730"
     CDS             <3454..3915
                     /locus_tag="PLV90_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOF97238.1"
                     /translation="VVIHPGYYAWSQERERAAGRFRQSLDELTAAADDLSVTFFVENM
                     GNWEYFFLRSCEDLPLVDGIGLALDVGHAHLNGCLDCFLTHPAAHFHLHDNDGTADTH
                     SPVGAGTIDFTAVMDAVARSNVIPIVEVETLDGVTASIDALTAIDAAARGG"
     gene            4081..4152
                     /locus_tag="PLV90_08735"
     tRNA            4081..4152
                     /locus_tag="PLV90_08735"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4113..4115,aa:Thr,seq:ggt)
     gene            4315..>4805
                     /locus_tag="PLV90_08740"
     CDS             4315..>4805
                     /locus_tag="PLV90_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOF97239.1"
                     /translation="MSPTIDRPPLIYLNNAATTWPKPPGVLAAITQSLALPVFGSGRT
                     TGSQGEDYVALAREAIAGLLAADPPEHVVFTSSATDSLNILIQGFLAGCDAGCHVLTT
                     ELDHNSVLRPLHELARQGRIRLTVLPFEDGAVSPDTVEAAITPDTRLMVTAHGSNVLG
                     SVQ"
BASE COUNT          838 a         1485 c         1532 g          850 t
ORIGIN      
        1 cgccccggcc gggattcgaa cccgggtcga aagctccgga ggctttcagg atatccacta
       61 ccctaccgag actgccatat gatgttgtcc tgtatggtat aaaaggtcag cgggagggcg
      121 ggtttgacgc ccttcgcgac tcgtagagat gccagatctt gcacgctccc tcgtggctga
      181 ccatgcaggg gccgatgggg gtgcgcgggg tgcaggcctt cgcaaagagc cggcagtcgg
      241 acggccgggc aacgccgcgc agcactttgt cgcatatgca ggccgaatgt ttgtccacgt
      301 gccggatctc gatatcgaac ttcttctgcg catcgtagcc ctcgaactca ggtttcaggc
      361 gaaggcccga ggccggtata acgggaaagc cgcgccactc gacgtcgaac ggctcgaagg
      421 cctcatacat gaggcgttgt gccttgacgt tcccctcccg ggagaccgcg cgcgggtagg
      481 cgttctccac ccggtgagcc ccttcgcgga cctggcgcac agccatgagg agaccgagga
      541 ggatatcctc cggctcgaac cccgcgacga cctgcgggac gggaaactgc tcgtactcct
      601 tgtagcccat cacggcacag acgtgcccgg ggagaaggaa cccctcgagc gccgcctcgc
      661 cccgggcaag gagccacgcc atagccggcg ggacgagccg gtggcaggag aggatcgaga
      721 agttctccgg cgggcgggag aggatcgtgg cggcgaccgt cggcgcggtg gtctcgaacc
      781 cgaccgatat gaagacgacc tcccggtcgg gctccctctc tgcgatctcc accgccctgt
      841 gaatgccctg caccacccgg acatccccgc cgctcgactc aagcgagccc ttcgacccgg
      901 ggacacgcag caggtcgccg taggtggcga cggtgcagcc ccgttccaca aggtcgagcg
      961 cggcgtcgat ctcgccctgc ggcgtgatgc agaccgggca ccccggtccc atcacgatct
     1021 tgagcctctc ggggaggatg ctccggagcc cggcgcgggc gatcgccgcc tcgtgggtgc
     1081 cgcagatgtg catgagcgtg atatcccggt cgacgagatc gaagagcgcc gtgagtatat
     1141 ccttaccaac cgccatggat cctcatacat atctctcgcc gatagggcat attcgtgccg
     1201 gtagcgggca gacccgggct caggtcgccc ggtagaatac gcccctctgc tcccgcacgg
     1261 gctcgaccgc ccccttctcc tccaggatgc ggaggtgctt cgtcacctcg gccggccgga
     1321 ggccggatat ccgggagagg tcggcgacgg tgcaggggcg gcgccggagc gtggcgagca
     1381 gcatatcggc gacgtcgcgc atctccggcg ccgttgccgg ttcgcacgat gccgccccga
     1441 tcacctccgc gttcccgccg atgatcgtcc tggcccgctc aagggtccgg ggcgacgccc
     1501 gccggaccct gatatcggtg ccgggccggt cgagcgtgtt cacctggacg cggtcgggcg
     1561 cgatgacggc gacggcgtcc ctcaggcgga ggagctcctc atccgtatcg ttcttgcccg
     1621 ggacgatgaa gacctcaagc cagatgacgc ccggaaactc ccgggagaag gcgacgagac
     1681 ccgcgacgac ctgcccggcg gtgatgccgg gggaggggcg gttgatatcg cgaaagatat
     1741 cttccgagac cgcatcgagc gacggcacga caaggtccgc ccgcatgagg gcggcccgaa
     1801 catcggggtc cacaaaaagc gcgccgttgg tcaggaccgc aacacggtag cggggataac
     1861 ggtctttgat gaacgatatg atatcgccga tcccggagtg aagcgtcggt tccccggagc
     1921 cggcgaacgt gacgtagtcg atatccggcg cctttgcaag gcagtcgtcg agctccgcga
     1981 tgacccgatc cgtcgggacg tactcgcgcc gttcaagggt gaggacggtc gttctcccgc
     2041 actcgcagta ggcgcagtca aacgtgcagg tcttctgcgg caccaggtcg atcccgagcg
     2101 accggcccag gcgccgggag gggaccggac cgaagaggta acggtatgcc atggggtgtc
     2161 accagactgg tttggggtga aggttcatca gtcttcaccc cggggcaaca gcgggcgttc
     2221 tgctctcatg tatcgccgat cttgagcatc ctcttgcaga actggtcgac cgatgagaag
     2281 attgcctggt tgatgccctc cgggcgggca gggagatcgg cggtggtggc aaaccggagc
     2341 aggtcggagt agtaactcag cgaaggatac ttccggcgga actcgtcgag gcagaggttc
     2401 gcctgctcct tccttccccg gagaaaggcg tagacgtaat cgatgagaca gaggacgtgg
     2461 cccggcatct tcttctcttc cgcaagcgtc ctaaacccgt tgaactcccc ctcccggggg
     2521 aggtagaggg tggttcggag accgtagtag atgggctcga ggtcgtcggg gaaccgctgg
     2581 agcatgtcgc tgatgaggcc aagggcatct gcaaggtcgc cctggtcgaa cttgaggttg
     2641 taggcctccc ggtagaagag catgtcgccc gggaaccgga cgaggccgat ctggagcagt
     2701 ttcaccagcg ccacgctgtt gttctcctgt tttgcccgtg aaagcccgat ctccaggaac
     2761 cagcggttat ccggcatcca ctcggtcgca aggaagatca tcatccagaa cggttcggcg
     2821 agccaccggt cctgctcgag ctctcccctg accagccgcc agagccccgg ccggagctgc
     2881 ccgagttcgt cgaggtgctg gcgtgcgagc tttaagtagt tccatcgctc cgcggccgag
     2941 tagccgggtt cggccgaccc ttccttgtag agggtatagt agcagtaggc gtgccagcac
     3001 cccaccacgg cgtcgtgcct cctgtacggc tggaggtagg ccgccgccgt aagcggcatc
     3061 ccgatcttca tcagcttgac ggcggctgca aggtggacgc cggccttccg ctctttcttc
     3121 cgtttcatgc cgcaggcgat ctcgcagaca cggttgacga acttcacgtc tcccgcccgt
     3181 ttgctgacat caaagacccg gtatgcgata gcgtcgatga actcgtcatg cgccgcatcg
     3241 gggatgggga ccccggcacg gtccaggatc gtgacgactc ttccaaagaa tgaggggaag
     3301 ttctcgtcga tggcttcggc gttggactcg atgaaggaga agatctcggc cttnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtggtca tccacccggg ctactacgcg
     3481 tggagccagg agcgggagcg ggcggcggga aggttccggc agtcgcttga cgagcttacg
     3541 gcggctgccg acgatctctc ggtcacgttc tttgtggaga acatggggaa ctgggagtat
     3601 tttttcctgc gctcctgcga ggacctgccg ctcgtcgacg ggatcgggct tgcgctcgac
     3661 gtcggccacg cgcacctgaa cggctgcctg gactgtttcc tcacccaccc tgcggcgcat
     3721 ttccacctgc acgacaacga cggcaccgcg gacacgcact ccccggtcgg cgccggaacg
     3781 atcgatttta ctgccgtcat ggatgccgtc gcgaggagta acgtcatccc catcgtcgag
     3841 gtggagaccc tcgatggggt gaccgcaagc atagatgcgc tcacggcgat cgatgccgcg
     3901 gcgcggggcg gatagggccg gccgggcaac gatccggcac gccgttctcc cggcatcggc
     3961 cgtaaccttt tatagggaaa aactccaaca tagttggtgc gttgtgcatt agtggctcag
     4021 ccggtagagc gcgttcctgg taaggacggt gccgaaaccg cctggttctc acatctcggc
     4081 gcctcggtgg catagcggta tcgcgcttcc ttggtaagga agaagtcgcg ggttcgattc
     4141 ccgcccgagg ctttatattt tgagttagaa tctcttttcc gggcgttatc tcatcttttc
     4201 ggtatatcgg gattgaggac cgggcagcat atatattgct attcccgaac gtggtgggag
     4261 aaccggatgg gagaaacgat ccctttttga ccgcggggga cagatcctct ggaaatgagt
     4321 cccacgattg accgtccgcc gctcatctac ttgaacaacg ccgcgacgac ctggccgaaa
     4381 cctccgggag tgcttgcggc catcacgcaa tcccttgccc tcccggtctt cgggtcgggg
     4441 aggaccaccg ggagccaggg cgaagattac gtcgcgctgg cacgggaggc gatcgcaggc
     4501 ctccttgccg cggacccgcc ggagcacgtg gtcttcacat cgagcgcgac cgactcctta
     4561 aacatcctca tccagggttt cctcgccggg tgcgatgccg gctgccatgt cctcacgacc
     4621 gaactcgacc ataactcggt cctgcggccg ctccacgagc ttgcgcggca gggccgcatc
     4681 cgattgacgg tcctcccctt cgaggacggc gccgtctccc ccgatacggt cgaggcggcc
     4741 ataacacccg atacccggct catggtcacg gcccacggga gcaacgtgct cgggtcggtg
     4801 cagga
//
LOCUS       DAOSII010000132         4791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_15806_length_4791_cov_42.598184, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000132 DAOSII010000000
VERSION     DAOSII010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15806_length_4791_cov_42.598184"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="PLV90_08745"
     CDS             <1..1152
                     /locus_tag="PLV90_08745"
                     /inference="COORDINATES: protein motif:HMM:TIGR04275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97240.1"
                     /translation="GPAGATAITSGPGDNEFPAVSGNTIVWFDNTTKSVKVYDVASGR
                     SLTIPGNNTYALVSMRQVDVSEDYVVWAGMSAATMTSDIYLYDIASGSVTPLTSGQGY
                     QMFPAIMDNLIFWDQIDMATGKGEIYWYNIANGHTGSLLKNSSINQLFPAAGGGFVTW
                     LDDGKVSGRLDFRWSSLQTGNITRLISTSSVISPPAVSSDGRWIVWTAMDEAGNAPYL
                     RMLDTTTKKIQKITGEGAMPANPAVDGDFIVWTDYRNGNGDIYLYDIQSGQERQLTSE
                     RAEEAFPDISDGRIVWMGNNDGYWNIYSATVGGRAQPTVAPTGFPTVAPTGFPTVAPT
                     GFPTVAPTGFPTFAPTGFPTPAPTGFPTFAPTGHHTEEPHHTEEPHHFT"
     gene            complement(1370..2686)
                     /locus_tag="PLV90_08750"
     CDS             complement(1370..2686)
                     /locus_tag="PLV90_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOF97241.1"
                     /translation="MNESNDVFIARGPVLIAGATINGSTVDIAVDETGTIAGIGEGIR
                     REHDVDVIIDGSDRLAFPGLVNTHTHAAMTLLRGYGDDMPLQEWLSQKIWPLEAHLTG
                     DDVYAGTRLACLEMIRSGTIAFNDMYFFMDRAAQAVDDMGIRATLAYGFIDLGNVEKR
                     EAEIKATESLVTRIKSLANPRIRAAVGPHSVYTVSPEGLSWCADFAREQEIGIHVHLS
                     ETEKEVVDCVAQFGKRPAAHLDDCGCLTPRTVAAHCCWLDEAECRLLGKRGVSASHNP
                     ASNMKLAVNRAMPYHWLKQHGANVALGTDGCASNNNLDLMEEMKFAALLQKFAWNSPT
                     LLSAGEAVAMATTAGARALGTGPGTLIVGAPADIILLDSRAVCNTPLHHADSNAVYAC
                     NGGAVMTVLCQGRVLMHEREVAGEEEIVREAAAAAQALVGRAREAA"
     gene            complement(2683..3354)
                     /locus_tag="PLV90_08755"
     CDS             complement(2683..3354)
                     /locus_tag="PLV90_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HOF97242.1"
                     /translation="MLGIIGGTSLLFADLPPLEETTVATPYGRAEVRTGAFALLMRHQ
                     HNLPPHRINYRACLAAFAILGVDTVIAFGSAGSLRPDIPPGSIVIPTDYLSATDIPSI
                     HECTIGHICPRLDADLIRSLGELIPEARVGGVYAQTRGPRLETVAEVRALACAADLVG
                     MTVASEATLAMELDMRFAAICTVDNYANGLGETALTFDQVLETSRANARRMEKILENI
                     VERYT"
     gene            3536..4660
                     /locus_tag="PLV90_08760"
     CDS             3536..4660
                     /locus_tag="PLV90_08760"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOF97243.1"
                     /translation="MGRFLMVGEDAIRAGRCTDVYFRRVVDVMEKDGVNPRVTMEVTA
                     TALPDPWGVFCGLDDVLRLLEGIPVDVDAMPEGSIFSANEPVLRISGRYRDFAVYETA
                     ILGFLCHASGVASAAAHVRLAAGGRPVYSFGSRRQHPAIAAMIERAAWIGGVDAASNT
                     CAPEGIALAGTMPHAFVMCYPRPEDAWSAFAREAAPEVPRIMLCDTFSDERAEAVRAA
                     ACGATAVRLDTPRSRRGDMRAIIEEVRWELDVHGYPDVQIFLSGGLSREDVAAYRDVA
                     DAFGVGGAIANAPVIDFAMDIVEMEGRPHAKRGRRSGAKQVYEMAGRRVTLPLEAPAP
                     EGGVGLLSRFIEGGAIRARPKMEDARERVLSRLSGLARRG"
     gene            4719..4790
                     /locus_tag="PLV90_08765"
     tRNA            4719..4790
                     /locus_tag="PLV90_08765"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4751..4753,aa:Ala,seq:ggc)
BASE COUNT          803 a         1462 c         1655 g          871 t
ORIGIN      
        1 ggaccggccg gggcgaccgc gatcacgagc gggccggggg acaacgagtt tcctgcggtc
       61 tcagggaata ccatcgtctg gttcgacaac actactaaat cggtcaaggt ctatgatgtt
      121 gcatccgggc ggagcctgac gattccgggg aacaacacct atgcgctggt atccatgcgg
      181 caggtggatg tctcagagga ctacgttgtc tgggccggca tgagcgccgc gacgatgacg
      241 tctgatatct acctttacga tattgcaagt ggttcggtga caccgctcac gagtggccag
      301 ggctaccaga tgtttccagc gataatggat aacctcatct tctgggacca gatcgatatg
      361 gcgaccggga agggcgagat ctactggtat aacattgcta acgggcacac cggttcgctt
      421 cttaaaaatt catcgattaa ccagctcttc ccggccgctg gcgggggttt cgtaacatgg
      481 ctcgatgatg ggaaagtcag tggtcgcctc gattttcgct ggtcttcact ccaaaccggg
      541 aatataacac gcttgatctc gacgagcagt gtaatcagtc cccccgcggt atcgagcgat
      601 gggagatgga tcgtctggac tgctatggat gaggctggga atgctcccta cctgcgcatg
      661 ctggatacca ccaccaagaa gatacagaag atcaccggtg agggagccat gccggccaat
      721 cccgccgtcg atggcgactt tatcgtctgg accgattacc ggaacggaaa cggcgatatc
      781 tatctctacg atatccagag cgggcaggag aggcagctca catccgagag agcagaagag
      841 gcgttcccgg atatatccga tggcaggatt gtctggatgg gcaacaacga cggttattgg
      901 aacatatact ctgccaccgt cggcgggaga gcacagccga ccgttgcgcc taccgggttc
      961 ccgaccgttg cacctaccgg gttcccgacc gttgcaccta ccgggttccc gaccgttgca
     1021 cctaccgggt tcccgacctt tgcgccgacc gggttcccga cccccgcacc gacggggttc
     1081 ccgacctttg caccgaccgg acaccacact gaggaaccgc atcacaccga ggaaccgcac
     1141 cacttcacct gatggggaga gcgcatcgtc cctggcgggg cgatctcccg cgcctttgca
     1201 ggcggtacgc cggtcctcgt caggtttagc ggtgacgggg acaacgtact gccgctctcc
     1261 aggagcgatg tgccctgccc ggatcgtggg cggtcccggt gcgccgggag aaaagacagc
     1321 cggcgttccg ggccatacga ggggctgccc ggaacaaccc ccgtgcggat caggcagcct
     1381 cgcgagcccg cccgaccagc gcctgcgccg ccgccgcggc ctcccggacg atctcttcct
     1441 cgcctgccac ctcccgctcg tgcatgagaa cccggccctg gcagaggacg gtcatgaccg
     1501 cgccgccgtt gcaggcgtag acggcgtttg aatcggcgtg atggaggggg gtgttgcaga
     1561 ccgcccggga gtcaaggagg atgatatcgg ccggcgcacc gacgatcagg gtccccgggc
     1621 cggtgccgag cgcccgggcg cccgcagtcg tcgccatcgc gaccgcctcg cccgcggaaa
     1681 gcagggtcgg cgagttccag gcaaacttct gcaggagggc ggcaaacttc atctcctcca
     1741 tcagatcgag gttgttgttc gaggcacacc catcggtccc gagggcgacg ttcgccccgt
     1801 gctgcttcag ccagtggtag ggcatggccc ggttgacggc aagcttcatg ttgctcgccg
     1861 ggttgtggga tgccgagacg ccgcgcttgc cgaggagccg gcactcggcc tcgtcgagcc
     1921 agcagcagtg cgcggcgacg gtccgggggg tgaggcaccc gcagtcgtca aggtgcgccg
     1981 cggggcgttt gccgaactgt gcgacgcagt cgacgacctc cttctcggtc tcggagagat
     2041 ggacgtggat gccgatctcc tgttcccgtg caaaatcggc gcaccaggaa agaccctccg
     2101 gcgagacggt gtagaccgag tgcgggccga cggctgcccg gatccgcggg tttgcaagcg
     2161 attttatgcg ggtgacgagg gattccgtcg ccttgatctc ggcctcccgc ttctcgacgt
     2221 tcccgagatc gatgaacccg taggcaagtg tcgcccggat acccatatcg tcgaccgcct
     2281 gcgccgcccg gtccatgaag aagtacatat cgttgaacgc gatcgtgccg ctccggatca
     2341 tctcaaggca ggcgagcctc gtgccggcgt agacgtcgtc gccggtaagg tgggcctcaa
     2401 gcggccagat cttctgcgag agccactcct gtagcggcat gtcgtccccg tagccccgga
     2461 gcagggtcat cgcagcgtgg gtgtgggtgt tgacgaggcc ggggaaggcg agccggtcgg
     2521 agccgtcgat gatgacgtcg acgtcatgct cgcgtcggat cccctccccg atcccggcga
     2581 tcgttcccgt ctcgtcgacg gcgatgtcca cggtcgatcc gttgatggtg gccccggcga
     2641 tcagcaccgg tccccgtgcg ataaatacgt catttgactc gttcatgtgt atctctccac
     2701 gatattctcc aggatcttct ccatccttct ggcattcgcc cgcgaggtct cgagcacctg
     2761 gtcaaaggtc agggctgtct ccccgaggcc gtttgcgtag ttgtccacgg tacatatggc
     2821 ggcaaaccgc atatcgagtt ccatggcgag tgtcgcctcg cttgcaaccg tcatcccgac
     2881 gaggtccgct gcgcaggcga gcgctcttac ctcggcgacc gtctcgaggc gcggcccccg
     2941 ggtctgggcg tagaccccgc ccacccgggc ctccgggatg agttcgccga ggctgcggat
     3001 gaggtccgcg tccagccggg ggcagatgtg gccgatggtg cactcgtgga tggacggtat
     3061 atcggtggcg ctgaggtaat cggtggggat gacgatcgag cccggcggta tatccggtct
     3121 cagggagccg gcggaaccga acgcgatgac cgtgtccacg ccgaggatgg cgaacgcggc
     3181 aagacacgcc cggtagttga tgcggtgcgg cgggaggttg tgctggtggc gcatgaggag
     3241 cgcgaaggct ccggtgcgga cctcggccct gccgtagggg gtcgcgacgg tcgtctcctc
     3301 gagcggcggg aggtcggcga agaggaggct cgtgcctccg atgatcccga gcactatacg
     3361 caccccgcac ggtggtgcag gccgtcccgg cgggccggga gagccgtagc ggcatacgct
     3421 cgtccgtgtg acatgctatg tagagggttg gcgcccggat gggaaaattc catctatacg
     3481 gtgttgcgga atcggttcgc aagagtttac ccctccggcg acgaccggat caggcatggg
     3541 caggtttctg atggtcggcg aggacgccat ccgggccggg cggtgcacgg acgtctactt
     3601 ccggcgcgtc gtggacgtca tggagaagga cggggtcaat ccccgcgtca cgatggaggt
     3661 gacggccacg gcgctcccgg acccgtgggg ggtcttctgc ggccttgacg acgttctccg
     3721 gctgcttgag ggcatcccgg tggatgtgga tgcgatgccg gagggttcga tcttctctgc
     3781 aaacgaaccg gtcctccgta tatcggggcg ctaccgggat tttgcggtct acgagacggc
     3841 gatactcggg tttctctgcc acgcgtcggg ggtggcgtcg gcggcggctc atgtccggct
     3901 tgccgccggg ggccggccgg tctactcctt cggctctcgc cggcagcacc cggcgatcgc
     3961 ggcgatgatc gagcgggcgg cctggatcgg cggggtggac gccgcgagca acacctgtgc
     4021 gccggaggga atagcgctcg cggggacgat gccccacgcg tttgttatgt gctatccacg
     4081 gcccgaggat gcctggtctg cgtttgcgcg ggaggctgcc ccggaggtgc cgcggatcat
     4141 gctctgcgac accttctccg atgagcgggc cgaggcggtc agggcggcgg catgcggggc
     4201 gacggcggtg cggctcgaca cgccgcggtc ccggcggggg gatatgcggg cgatcatcga
     4261 ggaggtgcgc tgggagctcg acgtccacgg ctacccggac gtgcagatct tcctctcggg
     4321 cgggctctcg cgcgaggacg ttgcggcata ccgtgacgtc gcggacgcct tcggcgtcgg
     4381 gggggcgatc gcgaacgcgc cggtgatcga ttttgccatg gatatcgtcg agatggaggg
     4441 gaggccccac gcgaagcgcg ggagacggag cggcgcaaag caggtctacg agatggccgg
     4501 ccggcgggtc acgctgcccc ttgaggctcc tgcgccggag ggcggggtgg ggctcctctc
     4561 ccggttcatc gagggcggtg ctattcgcgc acgcccgaag atggaggatg cgcgggagcg
     4621 ggtgctctcc cggctttccg gccttgctcg ccggggatag gggagcggcc ggccacaacc
     4681 ctttttatgg gatcacgatc aacatagtag ggtaacaggg gccgatagat cagggggaga
     4741 ttgcttcctt ggcatggaag aggccgcggg ttcaattccc gctcggtcca t
//
LOCUS       DAOSII010000133         4771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_15885_length_4771_cov_55.875742, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000133 DAOSII010000000
VERSION     DAOSII010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15885_length_4771_cov_55.875742"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2012)
                     /locus_tag="PLV90_08770"
     CDS             complement(<1..2012)
                     /locus_tag="PLV90_08770"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOF97244.1"
                     /translation="MEIVGLPIPPDLLADYTGRGITELYPPQAACVEAGLFAGKNLLI
                     AIPTASGKTLVAEMAMHHQVGRGGKCLYIVPLRALASEKFEEFSGKGLRVGIATGDFD
                     RRDEYLGRNDIIVATSEKVDSLLRNRTPWLAEVTLLVLDEVHLIDDPSRGATLEMVIA
                     KLRHKNPAIQIIALSATIGNPGDLAGWLGAELVESDWRPVDLREGVFFWNGIRFADRD
                     REVERRSKYEDLDLVLDTVAEGGQCLVFVSSRKNAEAFAKRAASGLKLKNPILAGYAD
                     KLRSSASTDMGKTLAACVAQGAAFHHAGLAREERQIVEAGFREGQIRVIASTPTLAAG
                     LNLPARRVIVRDYLRFNAGEGMVPIPVREYRQMAGRAGRPHLDPYGEAVLIAKSEEMV
                     DELFDCYINAPAEDVRSRCADEAVLCTHILSLIATGFAREADEVFAFMDGTFYAHQGE
                     SPRALSRAVHRALEFLREAEMITEVGEWIEATEYGSLVSRLYIDPRSAEVIVAAMIDR
                     QEYTDIGFLHLLCSTPDMPTLYIRRDDMYLLDRFLADHRDDLWTDIPWDAGEGFDRSL
                     KTALLLADWADEVGEDTICERYRVGPGDIYGMVESVAWLVHAARSLAKLFAPHLAAPI
                     EEMELRTKHGIKKELLPLIRLHGIGRVRARRLFNNNLGSIEALRAAG"
     gene            complement(2095..2637)
                     /locus_tag="PLV90_08775"
     CDS             complement(2095..2637)
                     /locus_tag="PLV90_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOF97245.1"
                     /translation="MTRQELKVTSARLGVLIGKSGSTKREIEEKTGTILRIDSEEGMV
                     TIEAEDPVAVMTTTNVVSAINRGFSPERAYRLLEDEDMMFDVIDLADLSGTTRHLERL
                     RGRIIGKAGTSRAQIEDMTGTELSVHGKTVAIIGLPDQVETARKAVEMLIQGVPHEHV
                     YAFLDRKKKEAKQAMLEYYS"
     gene            complement(2659..3435)
                     /locus_tag="PLV90_08780"
     CDS             complement(2659..3435)
                     /locus_tag="PLV90_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase RIO"
                     /protein_id="HOF97246.1"
                     /translation="MGHDEMHERFDREIEAMGVRIKDADALKVRDDVFDEVTLVALYK
                     LVHKKLISVIGGPISTGKEANVFYGERDKRGIAIKIYRIQTANFKAMTEYLAGDRRFA
                     SIRGSRKGIVFAWTKKEYSNLARAHEVGIPVPEPLAFDRNILLMEFLGREEVPYPQLR
                     NAEVADYGVTYGEILGYIERLYQEARLVHADLSEYNILYYEKPYVIDMGQAVTLDHPR
                     ASAFLIRDIRNINRYFSRYCDVQDEQEIIGAVTGSRGRER"
     gene            complement(3443..4390)
                     /locus_tag="PLV90_08785"
     CDS             complement(3443..4390)
                     /locus_tag="PLV90_08785"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOF97247.1"
                     /translation="MDPYELATRNTVEVVTDEELRALIDRPVRRVYTGYEPSGEIHLG
                     HMVTVNKLMDLQKAGFEVTVLIADLHAFLNRKGTMDEIREIAKYNRRCFEGLGLVGAK
                     YVLGSDVQLTPEYELAVLELSQAITLNRAKRSMDEVGRQMDNPTVSQMVYPIMQMVDI
                     ATLKVDAAVGGIDQRKIHMLAREHLPSIGYPAPVCIHTPIINGLDGKKMSSSAGNVIS
                     VADSAEDIRQKMKKAFCPPEVENNPVLEILRYHIFPRAGTVTIRRPAKFGGDREFAAY
                     NDLERAYAAGEIHPLDLKNAAANHLIDILAPVHDYVCSR"
     gene            complement(4445..>4771)
                     /locus_tag="PLV90_08790"
     CDS             complement(4445..>4771)
                     /locus_tag="PLV90_08790"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314759.1"
                     /note="catalyzes the formation of 3-phospho-D-glycerol
                     phosphate from D-glyceraldehyde 3-phosphate in glycolysis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOF97248.1"
                     /translation="REEVLKVFENHSRIGLVRKATGIKSNAQLREYAQDLGRPRADVW
                     ENGIFEESVSVLGGKEFYCFQAIHQEADVIPENIDCIRALMGTVKDPEESIRMTNKAL
                     GFVAIR"
BASE COUNT          773 a         1530 c         1482 g          986 t
ORIGIN      
        1 ccggccgcgc ggagcgcctc gatcgacccg aggttgttgt tgaagagccg gcgcgcccgg
       61 acgcgcccga tcccgtgaag cctgatgagg gggaggagtt ccttcttgat gccatgtttg
      121 gtccggagct ccatctcctc gatgggcgcc gcaaggtgcg gggcaaagag tttcgcgaga
      181 ctccgggcag cgtggaccag ccaggcgacg ctctccacca taccgtatat gtcgcccggg
      241 ccgacgcggt agcgctcgca gatcgtatcc tcgcctacct cgtccgccca gtcggcaagg
      301 aggagcgcgg tcttgaggct ccggtcgaac ccctcgcccg catcccaggg aatatccgtc
      361 cagaggtcgt cacggtggtc ggcgaggaac cggtcgagga ggtacatgtc gtcccgccgg
      421 atgtagagcg tcggcatatc gggtgtcgag cagaggaggt gcaggaaccc gatatccgtg
      481 tactcctgcc ggtcgatcat cgccgccacg atcacctcgg cgctccgggg atcgatgtag
      541 agccgggaga cgagcgaccc gtactccgtg gcctcgatcc actccccgac ctcggtgatc
      601 atctccgcct cgcgcaggaa ctcaagcgcc cgatggaccg cccgcgagag tgcacggggg
      661 ctctccccct ggtgggcgta gaacgtcccg tccatgaacg cgaacacctc atccgcctcg
      721 cgggcgaatc ccgtcgcgat gagcgagagg atgtgggtgc agaggaccgc ctcatccgcg
      781 caccggctcc ggacgtcctc ggccggcgcg ttgatgtagc agtcgaagag ctcgtcgacc
      841 atctcctcgg acttcgcgat caggaccgcc tcgccgtagg ggtcgaggtg cggtcgcccg
      901 gcccgcccgg ccatctgccg gtactcccgg acggggatcg ggaccatccc ctccccggcg
      961 ttgaaccgga ggtagtcgcg gacgatcact cgccgggcgg ggaggttcaa gcccgccgcg
     1021 agcgtcggtg tggaggcgat caccctgatc tgcccctccc ggaaccccgc ctcgacgatc
     1081 tggcgctctt cgcgggcaag gcccgcgtgg tggaacgccg caccctgcgc gacgcaggcg
     1141 gcaagtgtct tccccatatc ggtcgaggcg ctcgagcgta gcttatcggc atagccggcg
     1201 aggatcgggt ttttgagctt caaccccgat gccgcacgct ttgcaaacgc ctcggcgttc
     1261 ttgcgggagc tgacgaagac caggcactgc cccccctccg cgaccgtatc gaggacgagg
     1321 tccagatcct cgtatttgct ccggcgttcg acctcgcggt cgcggtcggc aaagcggatg
     1381 ccgttccaga agaagactcc ctcccggaga tccaccggcc gccagtcgct ctcgacgagc
     1441 tccgcgccca gccacccggc gagatccccg gggttcccga tggtggcgga gagggcgatg
     1501 atctggatcg ccgggttctt gtgccggagc ttcgcgatca ccatctcgag cgtcgcccca
     1561 cgggaggggt cgtcgatgag gtggacctcg tcgaggacga gcagggtgac ctccgcgagc
     1621 cacggcgtcc ggttccggag gagcgagtcc accttctcgc tcgtcgcgac gatgatgtcg
     1681 tttctcccca ggtactcgtc ccttcgatcg aagtcgccgg tggcgatgcc gacacggagg
     1741 ccttttcccg agaactcctc gaacttctcg ctcgcgagcg ctcgcaaggg gacgatgtag
     1801 aggcacttgc cgccccggcc gacctggtgg tgcatcgcca tctcggcgac cagggtcttg
     1861 ccgcttgccg tcggtatggc tatgaggagg ttcttccccg caaaaagacc cgcttcgacg
     1921 caggctgcct gcggcgggta gagttccgtg atcccccgcc ccgtgtagtc ggccagaaga
     1981 tccggcggga tggggaggcc gactatctcc atgtataccc cttcatttcc actgcagact
     2041 gtattttctt cgtgttatca tcggggtgtt cagggccggt ctgctccggc cgcatcacga
     2101 atagtactcc agcatcgcct gttttgcttc cttcttcttc cggtcgagga aggcgtagac
     2161 gtgctcgtgg ggcacgccct ggatcagcat ctccaccgcc tttcgtgcgg tctcgacctg
     2221 gtccgggaga ccgatgatag cgacggtctt tccgtgaacc gatagctccg tgccggtcat
     2281 atcctcgatc tgggcgcggg atgtgcctgc tttccctatg atccggcccc ggagccgctc
     2341 gaggtgccgg gtcgtgcccg agaggtcggc gaggtcgatg acgtcgaaca tcatgtcttc
     2401 gtcctcaagg agccggtatg cacgctccgg cgagaacccc cggttgatcg cggagacgac
     2461 gtttgtcgtc gtcatgacgg cgacgggatc ctccgcctcg atcgtcacca tcccctcctc
     2521 gctgtcgatc cggaggattg tcccggtctt ctcttcgatc tcgcgtttcg tggacccgct
     2581 cttgccgatg agtacaccaa gccttgcgga tgtaactttt agctcctgtc tggtcatggt
     2641 tatcgtgtct cccgatggtt atcgctcccg gcctctgctg ccggtgaccg caccgatgat
     2701 ctcctgttcg tcctgcacat cgcagtaacg ggagaaatac cggttgatgt tcctgatatc
     2761 ccggatcagg aacgcggacg cccgtgggtg atccagggtg accgcctgtc ccatatcgat
     2821 cacgtatggt ttctcgtagt agaggatatt gtattcggag aggtcggcat ggaccaggcg
     2881 tgcctcctga taaagccgtt cgatatagcc gaggatctcg ccgtacgtga ccccgtaatc
     2941 cgcaacttcg gcgttccgga gctgcgggta cggtacctct tcgcgcccga gaaactccat
     3001 caagaggatg ttccggtcaa acgcgagggg ttcggggacc gggatcccca cctcgtgcgc
     3061 ccgggcgagg ttgctgtact ctttctttgt ccaggcaaag acgatgcctt tgcgcgaccc
     3121 ccggatgctc gcaaaccggc ggtcgcctgc aaggtactcg gtcattgcct tgaagttcgc
     3181 cgtctggatg cggtagatct tgatcgcgat cccccttttg tcgcgctcgc cataaaagac
     3241 gttcgcctct tttccggtgc tgatagggcc gccgatgacc gagatcagtt ttttgtggac
     3301 aagtttgtag agcgcgacga gagtgacctc gtcaaagacg tcgtcccgta ccttcagtgc
     3361 atcggcatcc tttatccgga cacccatcgc ctcgatctcg cggtcgaacc gctcgtgcat
     3421 ttcatcatgg cccacggccg gatcacctgc tgcagacgta gtcgtgtacg ggggcgagga
     3481 tatcgatgag atggttggca gccgcgttct tcaggtccag gggatggatc tcgccggcgg
     3541 cgtaggcccg ctcgaggtca ttgtaggcgg caaactcccg gtctccgccg aacttcgcgg
     3601 gccggcggat ggtgaccgtc ccggcccggg ggaagatgtg gtagcgcagg atctcgagca
     3661 ccgggttgtt ctcaacctcc ggcgggcaga acgccttctt catcttctgc cggatatctt
     3721 ccgcggagtc ggcgaccgat atgacgttgc cggccgacga cgacatcttc ttcccgtcaa
     3781 ggccgttgat gatcggcgtg tggatgcaga ccggtgccgg gtagccgatc gaggggaggt
     3841 gttcccgcgc gagcatgtgt atctttcgct gatcgatccc gcccacggca gcgtccacct
     3901 tcaaggtcgc tatatcgacc atctgcatga tgggatagac catctgcgag accgtcgggt
     3961 tatccatctg ccgcccgacc tcgtccatgc tcctcttcgc ccggttgagg gtgatcgcct
     4021 gggaaagttc gaggacggca agctcgtact ccggcgtgag ctgcacatcc gacccgagaa
     4081 catacttcgc gccgaccagc ccgagcccct cgaaacagcg acggttgtac ttcgcgatct
     4141 cgcgaatctc gtccatcgtg cctttgcggt ttaagaacgc atgaaggtcc gcgatgagaa
     4201 cggtcacctc aaaccctgct ttctgcaggt ccatcagctt gttcaccgtc accatatggc
     4261 cgagatggat ctccccgctc ggctcgtagc cggtgtagac ccgcctgacc gggcggtcga
     4321 tgagcgcacg cagttcctcg tcggtgacga cctctacggt atttctggtc gcgagttcgt
     4381 atgggtccat tgaaaagagt gggtctgccg gggagttaat ggttcttccc cctggccctg
     4441 ccgttcacct gatggcgacg aacccgagcg ctttgttggt catccggatg gattcctccg
     4501 gatccttcac ggttcccatc aaggcgcgga tgcagtcgat gttctcgggg atgacgtcgg
     4561 cctcctggtg gatggcctgg aaacagtaga actccttccc gccgaggacc gataccgact
     4621 cctcgaagat gccgttttcc cagacgtcgg cccttggccg gccgaggtcc tgggcgtact
     4681 cccggagctg tgcgttgctc ttgataccgg tggccttgcg gacgagcccg atgcggctgt
     4741 ggttctcaaa gaccttcagc acctcttcgc g
//
LOCUS       DAOSII010000134         4760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_15929_length_4760_cov_47.225080, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000134 DAOSII010000000
VERSION     DAOSII010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15929_length_4760_cov_47.225080"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..742
                     /locus_tag="PLV90_08795"
     CDS             194..742
                     /locus_tag="PLV90_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOF97249.1"
                     /translation="MERDEALALLRRYVVSPSHIAHSYATAGIMKRVAEHLGEDAGTW
                     EIIGLLHDIDYDLVAGDMDRHGIEGHRILIENGVPEEIADIVRRHNHTHFSDYVRPVE
                     IALQAADSASGLIIACALVKGGAVSEVAPRTVKKKFKEKSFAAGCERERIRMIESLMD
                     METFFRLAIEGIMDIRTDIGLT"
     gene            782..1333
                     /locus_tag="PLV90_08800"
     CDS             782..1333
                     /locus_tag="PLV90_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate synthase family protein"
                     /protein_id="HOF97250.1"
                     /translation="MTGVDRARVIERMNEAMMLLTERMDARLIPETGMNIAYALPDAR
                     SKDDVAAVPGGIVGHADRVHPAGDIAFGADAYVARIVITAMRFDPAIRSAATIRFSEA
                     VLIEMENLLFEICSFDPEKEPPGVQTMDWGVASCCREGVPDVIYDRGAAGKEPTIRLL
                     GEDPVTVARNLLKLSNRITYAQL"
     gene            1346..1534
                     /locus_tag="PLV90_08805"
     CDS             1346..1534
                     /locus_tag="PLV90_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17e"
                     /protein_id="HOF97251.1"
                     /translation="MGIKPSYIKNLGEELIVKHRERFSGDFEENKHAVSEVATIGSKS
                     VRNRVAGYISRKINTRKR"
     gene            1545..2420
                     /gene="dapA"
                     /locus_tag="PLV90_08810"
     CDS             1545..2420
                     /gene="dapA"
                     /locus_tag="PLV90_08810"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOF97252.1"
                     /translation="MFEGILPAIITPFHRDSGMSLDIEGLRSNIESLLQRGIHGIVPC
                     GSTGESATLTFEEHEQVISKTVEVVNGRIPVLAGTGSNNTEEAIRLTRAAKDAGADGA
                     LVISPYYNKPNRSGLVKHFTKLADIGLPIVLYNVPGRTGQNLLPDLVIELARHPNIVG
                     VKEASGDIVQISRIIEGTRDEEFVVLSGDDAMTLPVLALGGAGVISVTANVDPGRMVQ
                     MYEAFRAGDLARARDLHFELSPLMRAMFIDTNPIPVKKAVGLSGMAAGPVRLPLDDLD
                     GQKTEQLRKVLASYD"
     gene            2413..3171
                     /gene="dapB"
                     /locus_tag="PLV90_08815"
     CDS             2413..3171
                     /gene="dapB"
                     /locus_tag="PLV90_08815"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOF97253.1"
                     /translation="MIKVAVSGALGRMGANIGRLVNEAPDLTLVGGIDVREGTLFETE
                     VVPAAKIDEFLKEKRPDVLVDFTVAAAAVENVRAAARNGVALVVGTTGFSPEQREVIR
                     GAVEGNVPAVISSNFSVGVNIFWKLVREAARELGDYDIEVTEAHHRHKKDAPSGTAKT
                     ILDILDEEIGNREKAYGRVGMTERKNEIGVHVIRGGDIVGDHAVLFAGNFECIEISHR
                     AYDRAVFAQGAVRAVRWVVGKEPRMYTMQDVLGI"
     gene            3253..3426
                     /locus_tag="PLV90_08820"
     CDS             3253..3426
                     /locus_tag="PLV90_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOF97254.1"
                     /translation="MPAVVDIEKCTACETCIDVCPASAITMEDGKAKVDPDLCVDCET
                     CVDECPSEAISME"
     gene            3473..4489
                     /gene="asd"
                     /locus_tag="PLV90_08825"
     CDS             3473..4489
                     /gene="asd"
                     /locus_tag="PLV90_08825"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOF97255.1"
                     /translation="MIHVGVLGATGAVGQRFVQLLADHPWFHLQTLTASERSAGKPYG
                     KVVNWRLDAPYPDNVSDIVVTPTTVESLKDCDLVFSALPADVAGPFETELANAGIAVC
                     SNARSHRMEPDVPLVIPEVNPDHLGLIDLQRDRGNGGFIVTNPNCSTIVLVLALAPLR
                     AFEFHDVRAATMQAISGGGFAGVPAMDIYDNVIPYIGSEEEKIETETAKIMGTFNGSE
                     VIPAPFKVSASCHRVPVIDGHTIAVWVDVRESVAEVKKAYATFKSPFSNLPLQPAKAV
                     ELFDQPDRPQPRLDRNRGRGMTVSVGRIREGLRFVALGHNTIRGAAGASVLNAELIHS
                     KKYL"
     gene            4504..>4760
                     /gene="albA"
                     /locus_tag="PLV90_08830"
     CDS             4504..>4760
                     /gene="albA"
                     /locus_tag="PLV90_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006325 - chromatin organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein Alba"
                     /protein_id="HOF97256.1"
                     /translation="MIKDNTVFVGNKPVMNYVLAVVTQFNNGAEEVAIKARGKAISRA
                     VDTAEIALNRFLENVSKKEIFTSTEMIDTDTGKTNVSSIEIV"
BASE COUNT         1027 a         1403 c         1480 g          850 t
ORIGIN      
        1 aggcgcgggc ggagtggggg caaacggcgc atgggttccc gccggcgggg ggggctcgcc
       61 tgcaatagtc actccgaacc ggtgttgcag ggatgaccgc cggggagcac cggataccgg
      121 gcgggactcc tcacccgccc ccaccggtgc aaaaacccgc acgcttatct ctgcgccgga
      181 taacccgtcc ctcatggaga gagacgaagc actggccctg ctccggcgct acgtggtgtc
      241 gccgtcacac atcgcgcaca gttacgccac ggcgggcatc atgaagaggg tcgccgaaca
      301 cctcggcgag gatgccggca cctgggagat catcggcctt ttgcacgaca tcgactacga
      361 cctcgtcgcc ggagacatgg accggcacgg catcgagggc caccggatcc tcatcgagaa
      421 cggcgttccc gaagagatcg cggatatcgt caggcgccac aaccacaccc atttctccga
      481 ttacgtccgg cccgtcgaga tcgcgctcca ggcggcggac agcgcatcgg gccttatcat
      541 cgcctgcgcc ctggtgaagg gcggggcggt cagcgaggtc gcgccccgaa ccgtgaagaa
      601 gaagttcaaa gagaagtcgt ttgccgccgg gtgcgagcgc gagaggatcc ggatgatcga
      661 gtccctcatg gatatggaga cgttctttcg cctcgcgatc gagggtatca tggatatccg
      721 taccgacatc ggcctcacct gagcggaatc cttatgagac gcccggcacg aggagtatag
      781 catgacaggg gtagaccgcg cgcgggtgat cgagcgcatg aacgaggcga tgatgctcct
      841 caccgagcgg atggatgccc gcctcatacc ggagacgggt atgaacatcg cctatgccct
      901 gcccgacgcg cggagcaagg acgatgtggc ggcggtcccg ggagggatcg tcgggcacgc
      961 cgatcgggtc caccccgccg gcgatatcgc gttcggtgca gacgcctacg tggcccggat
     1021 cgtcattaca gccatgcggt ttgacccggc gatccggagc gcggcaacca tccgcttctc
     1081 ggaagcggtc cttatcgaga tggagaacct cctcttcgag atatgctcgt ttgacccgga
     1141 aaaagagccg ccgggggtgc agacaatgga ttggggtgtc gcctcctgct gcagagaggg
     1201 cgtgcccgac gtcatctacg accggggcgc cgcagggaaa gaaccgacga tcaggctgct
     1261 cggggaagat ccggtcacgg tcgcacgcaa tcttcttaag ctgtcgaatc gcattacgta
     1321 tgcacaattg taagggaagg tccacatggg aataaagccg tcatatatca agaacctcgg
     1381 cgaggaactc atcgtcaagc atcgtgagag atttagcgga gattttgagg agaataagca
     1441 cgccgtctcc gaagtcgcca cgatcgggag caagtccgtc cgaaaccggg ttgccgggta
     1501 catctcaaga aagataaata ccaggaagcg ttaaacctca ctatatgttt gagggaatcc
     1561 ttccagcaat cattacccct tttcatcggg actcggggat gagtctcgat attgaaggat
     1621 tacggtccaa catagaatca ctgctgcaac gcgggatcca tggcatcgtc ccctgcggat
     1681 cgaccgggga gtccgcgacg ctcacgttcg aggagcacga gcaggtgatc tcaaagaccg
     1741 tcgaggttgt caacgggcgg ataccggtgc ttgcgggaac cgggtcgaac aacaccgaag
     1801 aggcaatccg gctgacccgc gcggcaaagg atgcaggcgc ggacggcgcc cttgtcatca
     1861 gcccgtacta taacaagccg aaccgctccg gtctcgtcaa acacttcacg aagcttgccg
     1921 atatcggtct ccccatcgtc ctctacaacg tccccggccg gacggggcag aacctcctgc
     1981 cggacctggt gatcgagctt gcccggcacc caaacatcgt cggcgtgaag gaggcaagcg
     2041 gcgatatcgt ccagatatcc cggatcatcg aggggacccg ggacgaggag ttcgtcgtgc
     2101 tctcgggcga cgacgcgatg acgcttcccg tcctcgctct cgggggcgcg ggcgtgatat
     2161 cggtgaccgc aaacgtcgac cccggccgga tggtgcagat gtacgaggcg ttccgcgcgg
     2221 gcgaccttgc ccgcgcacgg gacctgcact tcgaactctc gccgctcatg cgggcgatgt
     2281 tcatcgatac gaaccccatc cccgtgaaga aggcggtcgg gctctccggg atggccgcag
     2341 gaccggtcag gctgccgctc gacgacctgg atgggcagaa gacggagcag ctgaggaagg
     2401 tgctggcgag ctatgattaa ggtagccgta tccggggccc tggggagaat gggcgccaac
     2461 atcggccggc ttgtcaacga ggctcccgac ctcaccctgg tcggcggcat cgacgtgagg
     2521 gagggaacgc tctttgagac ggaggtcgtc cccgcagcga agatcgacga gttcctcaag
     2581 gagaagagac ccgacgtcct cgtcgacttc accgtcgcgg ccgctgccgt cgagaacgtc
     2641 agggccgccg cgagaaacgg cgtagcgctc gtcgtcggaa cgacagggtt ctctcccgag
     2701 cagcgggagg tgatccgggg cgcggtcgaa ggaaacgtcc ccgccgtgat atcgagcaac
     2761 ttctccgtcg gcgtcaacat cttctggaaa ctcgtgcggg aagccgcccg cgagctcggc
     2821 gactacgaca tcgaggtcac ggaggcccac caccgccaca agaaggacgc cccgagcggc
     2881 accgcaaaga ccatccttga tatactggac gaggagatcg ggaatcgcga gaaggcctac
     2941 ggccgtgtgg gtatgacgga gcggaagaac gagatcgggg tgcacgtcat ccgcggcggc
     3001 gacatcgtcg gcgaccacgc ggtcctcttt gcggggaact ttgagtgcat cgagatctcc
     3061 caccgcgcct acgatcgggc ggtcttcgca cagggtgccg tccgggctgt ccgctgggtc
     3121 gtcgggaaag aaccgcgcat gtataccatg caggatgtcc tcgggatctg agggaggccc
     3181 cctcgcccgc cttccggaaa cgaaatttat ttttacgccg ctagaaaaac atgtacgtct
     3241 ggaggagaaa aattgccagc agttgttgat attgagaagt gtaccgcctg tgagacctgt
     3301 atagatgtct gcccggcttc cgccatcacc atggaagacg gcaaagcaaa ggtcgatccc
     3361 gatctctgcg tcgattgcga gacctgcgtc gacgagtgtc cgtctgaggc aatctcgatg
     3421 gaatgaagaa acatactctt taatacctcc tacactacag atatttttgt ctatgattca
     3481 tgtaggagtg cttggtgcca caggagcggt gggacagcgc ttcgttcagc ttttagcgga
     3541 tcatccctgg tttcatctcc agacactcac cgcttctgaa cgaagtgcgg gaaaacccta
     3601 tggtaaagtg gtcaactggc gccttgatgc gccgtacccg gacaacgtca gcgatatcgt
     3661 cgtcactccc acaaccgtcg agagtctgaa agattgcgat ctcgtcttct cagcgctccc
     3721 tgccgacgtg gcgggaccgt tcgagaccga gcttgccaat gcgggcatcg ccgtctgcag
     3781 caacgcccgg tcgcaccgca tggaaccgga cgttccgctg gtgatcccgg aggtcaatcc
     3841 ggatcacctg ggcctgatcg atctccagcg ggaccgcggt aatggggggt tcatcgtgac
     3901 caacccgaac tgctcgacga tcgtcctagt gctggccctc gcacccctca gggcgtttga
     3961 gttccatgac gtgcgtgcgg caaccatgca ggcgatatcg ggaggcgggt ttgcaggcgt
     4021 ccccgccatg gacatctatg ataacgtcat cccctatatc ggctcagaag aggagaagat
     4081 cgagaccgag accgcaaaga tcatgggcac gttcaacggt tccgaggtga tccctgcccc
     4141 gtttaaggta agcgcaagtt gccaccgggt ccctgtcatc gacgggcata ccatcgcggt
     4201 ctgggtcgac gtcagggagt cggttgccga ggtgaaaaaa gcttacgcaa cctttaagtc
     4261 acccttcagc aatctaccct tacaaccggc aaaggcagtt gagctcttcg accagcccga
     4321 tcgtccgcaa ccccggcttg accgcaaccg ggggcgcggc atgacggtct ccgtcgggag
     4381 aatccgggaa ggattacggt ttgttgcact tgggcataac accatccgtg gggctgcagg
     4441 cgcatccgtc ctcaacgcag agctgataca ctcgaagaag tacctttaga cgaggtgggc
     4501 ttgatgatta aggataacac agtattcgtg ggaaacaaac cggtcatgaa ctatgtgctc
     4561 gcggtggtca cccagttcaa caacggagcg gaggaggttg cgattaaggc cagggggaag
     4621 gctatttcac gtgcggtcga cacagcggag atcgccctca accggttctt agagaacgtc
     4681 agcaagaaag agatcttcac atcgaccgag atgatcgaca ccgacaccgg aaagacgaac
     4741 gtctcaagca tcgagatcgt
//
LOCUS       DAOSII010000135         4708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_16149_length_4708_cov_42.285622, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000135 DAOSII010000000
VERSION     DAOSII010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16149_length_4708_cov_42.285622"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="PLV90_08835"
     CDS             complement(<1..911)
                     /locus_tag="PLV90_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOF97257.1"
                     /translation="MRSVEKKVEAVCQHSPDIVAFQEVTDRDLPLFREGLENFGLTHF
                     VDSLALVEIARVAYMAERLNDLRARNTNDPFQFAYSISRLSEQCSPVGETKDAGCLIA
                     SRYPLAPLNPLDFDIPWKQRVVSAVVSAPTGEFEVHNVHVPTAIGSRRNEIVKAETLV
                     GIYRHLAVRPARPRILCGDLHIPEAELPDGTIVPFYRDIIPDRTYTTASESDEYLGRP
                     RRWWYNAEMNVISGLAEYDLPDVFRKLHGYRAREYSWCPDRGPEDVPKCKRYDHIFAS
                     ARLNPTACWYLHDLRGQGLSDHSAVVM"
     assembly_gap    1484..1583
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1584..>4708)
                     /locus_tag="PLV90_08840"
     CDS             complement(<1584..>4708)
                     /locus_tag="PLV90_08840"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HOF97258.1"
                     /translation="LKAGEKQLQWDAGLVRGAGQIAVVKKTNGQIAKNPPGPTIKVGD
                     KVTWTYEVTNPGSGPLANVKVTDDKAGPIAGPPAGDANGDGKLDPGETWVYTKDDTAK
                     DTSGMPGGSYKNTVTVTADDQTAKPVTATDLSHYTSSTAGGGCACTADFTFSTTNRAP
                     PAGVQNPDPLSLRVYHFQGSVQTTGCPAGVAPTTWEWDFGDGKGWQDLGQNPTHTFAA
                     AKTYDVKLRVTLSNGVTCEIVKKVTVEAPPQPEGKGSIGDFVWNDRNQNGKQDSGEKG
                     LAGVSVALLDENDNPINGENGQPRRVTTKEDGKYVFDNLVEGKYRVRFDPPQGNWIHS
                     PMDNVQNDDDLDSDPGISGRTGIIVIKEGALVQLNWDAGFYNHAMQGTGQIGDLVWED
                     ENENGIQDGGEKGVPGVSVNLMDDQFMHIYTTATDANGKYLFTDLTAGDYYVVFGHKA
                     AQREAKFTKQNVDANTKDDVDSDASMIGRTDKITLQDNQKQLNWDAGLIFLPQTTISP
                     PPQGQGSIVGVTFNDQNGNGILDGGESGVPGVGVTLLAGPTPGESTPEAGTRSSASGR
                     ETSGITLRDDDRTDRISFAALADAGNLTAVNETTTDENGTYTFTNLTIGEYYLVFDPP
                     EGYAFTIPGEGSEFDPETGTAGPINIVKNDTRITLNIGLNRPPTVVTATANSTIGDLV
                     WNDTNGNGIQDRVEGGIAGAAVELYHANETLVNSTTTDADGAYLFSALPAGDYYLIFG
                     LPEGYNFTPADRGTDDARDSDADPATGRTATITLADNDTQLTWDAGANLTAAQTATIG
                     DFVWNDTNGNALQDEGEPGVPGVTVRLLNENGYPVPGAAETVVETTTGADGRYALKGI
                     VGTTYIVEVVAPDGLFFVLMNAGDDTRDSDVGFKTGRTGPFTLVGDDLTQDAGLTKFS
                     PGARYNTIGGFAWNDTNADGARDAGETGVSGVAVALIGADGTTARTATTDGDGAYRFT
                     DVVMGTYSVSFTLPEGYTFSPAGQDSTVDPGSGTTEQFEIGPNEEQLNWNAGLVPLAA
                     EVTPTPEVTATP"
BASE COUNT          730 a         1453 c         1383 g         1042 t
ORIGIN      
        1 tccatcacga ccgcggagtg gtcgctcaac ccctgccccc ggaggtcgtg caggtaccag
       61 cacgccgtcg ggttgaggcg cgcagatgca aatatgtggt cgtagcgctt gcacttcggg
      121 acatcctcgg gcccgcggtc ggggcaccag ctgtactccc tcgcccggta gccgtgcagt
      181 ttccggaaga catcggggag gtcgtactcc gcaaggccgg atatgacgtt catctcggcg
      241 ttataccacc accttctcgg ccgcccgagg tactcatcgc tctcggaggc cgtcgtgtag
      301 gtccgatcag ggatgatatc gcggtagaag gggacgatcg tcccgtcggg aagttccgcc
      361 tccggtatgt gaaggtcgcc gcagaggatc cgtggcctcg ctggcctgac ggcaaggtgt
      421 cggtagatcc cgaccagtgt ctcggccttc acgatctcgt tcctccggct gcctatcgcc
      481 gtgggaacgt ggacgttatg cacctcgaac tcgcccgtgg gtgcactgac gacggccgag
      541 accacccgct gtttccacgg gatatcgaag tcgagcggat ttaatggcgc aagggggtac
      601 ctgctcgcga tcaggcatcc ggcgtccttt gtctcgccga ccggcgagca ctgttcggag
      661 agacgcgaga tgctgtacgc gaactggaag ggatcgtttg tgttgcgcgc acggagatcg
      721 ttcaaccgct cggccatgta ggccacccgg gcaatctcca ccagagcaag gctgtcgacg
      781 aaatgagtta acccgaagtt ctccaatccc tcacgaaaga gcggcagatc ccgatccgtc
      841 acctcctgga aggcgacgat atccggcgag tgctggcaga ctgcttcgac tttcttctcc
      901 acgctcctca taccggagcg gaagagatcc acgttccatg aaatgagccg catagttctg
      961 gtacattgcg cgttggccct gcatgcacat taacacatcg agttgagagc ggcagcgcgg
     1021 cgccggcgct gcccgtgaca gcgctcgatc ccgcgggcca ctgcatgccc ggcaatccct
     1081 cccctatggt gctcacagta ccacgggcag cggccgacga cgggggatac cgggatcggg
     1141 cctcgcgaaa aggcgcgaaa ctcatgacgc aacggtgatc tgcctcacgt gcccccggat
     1201 gagacgatca ggcccactac cttcgccatg tacgggcttt acaggttcgt gtgcggaccg
     1261 ctcctccaaa aaactacatg atgcccatgg ggccacaggc agaccggatg aaaaagggga
     1321 agggcatttt catacgaaag ggatgattga gagacgaggc cttacaggga gcctcccgcg
     1381 caggttgtca ccccggcacc gccccctttc agtctcaccc cgcctctcct catctacggg
     1441 cggcggctat tctgcttccg gcgtcgtcgt cgcctccggt gtcnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnggtgtcg ccgtcacctc aggtgtcggg gtcacctctg
     1621 cggccagcgg cacgagcccc gcgttccagt tcagctgctc ctcgttcgga ccgatctcga
     1681 actgctccgt cgtaccgctc ccgggatcga cggtgctgtc ctggccggcg gggctgaagg
     1741 tgtagccctc cggcagcgtg aagctcacgg agtaggttcc catgacgaca tcggtgaacc
     1801 ggtaggcgcc gtcgccgtct gtcgtcgccg tcctggctgt cgtaccatcc gcgccgatga
     1861 gggccaccgc aacgcccgac accccggtct cgccggcatc ccgggcgccg tcagcgttcg
     1921 tgtcgttcca ggcaaacccg ccgatggtgt tgtacctggc tccgggtgag aatttcgtca
     1981 gccccgcatc ctgcgtgagg tcgtcgccca cgagcgtgaa gggtccggtt cggccggtct
     2041 tgaacccgac gtcgctgtcc cgcgtatcgt cgccggcgtt catcaagacg aagaagagcc
     2101 catcgggcgc cacgacctcg acgatgtagg tcgtgccgac gatgcccttg agggcgtagc
     2161 ggccgtctgc ccccgtcgtt gtctcgacga cggtttcggc cgcgcccggg accgggtaac
     2221 cgttctcgtt caggagccgg acggtgaccc cgggcacgcc gggttcgccc tcgtcctgga
     2281 gagcgttgcc gttcgtgtcg ttccagacga agtcgccgat ggtggcggtt tgtgccgccg
     2341 tcaggttcgc cccggcgtcc caggtgagtt gcgtgtcatt atcagcgagc gtgatggtcg
     2401 ccgtcctgcc ggtcgccggg tcggcgtcgc tgtcccgtgc gtcgtcggtc ccccggtcgg
     2461 ccggcgtgaa gttgtagcct tccggcagcc caaagatcag gtaataatcg cccgcgggga
     2521 gggcgctgaa gaggtaggcc ccatccgcat ccgtcgtcgt ggagttgacg agggtctcgt
     2581 ttgcatggta gagctcgacg gcggcgccgg cgatgcctcc ctcaaccctg tcctggatgc
     2641 cgttgccgtt cgtgtcgttc cagacgaggt cgccgatggt gctgtttgcg gttgcggtca
     2701 ccactgtcgg aggccggttg agcccgatgt tcagtgtgat ccgggtatcg ttcttcacga
     2761 tgttgatcgg gcccgcagtg ccggtctcgg gatcgaactc gctcccctct ccggggatgg
     2821 tgaaggcgta gccttccggc ggatcgaaca cgagatagta ctctccaata gtgaggttcg
     2881 tgaaggtata ggtgccgttc tcatcggttg tggtttcgtt gacggcggtc agattgcctg
     2941 catccgccag ggccgcgaat gatattctgt cggttctgtc atcgtcgcgc aaggttattc
     3001 cgctggtttc acgtccggaa gcgctggaac gcgtacctgc ttcgggggtg ctttctcccg
     3061 gcgtcggtcc ggcaagaagc gtcactccga cccccggaac gccggactcg cccccgtcca
     3121 ggatgccgtt gccgttctgg tcgttgaacg tgacgccgac gatggaaccc tgtccctggg
     3181 gagggggact tatcgtcgtc tgcggtaaaa agatcaatcc ggcatcccaa ttgagctgct
     3241 tctggttatc ctgcagcgta atcttatccg ttctcccgat catgcttgca tcgctgtcta
     3301 cgtcgtcttt tgtgttggca tcgacattct gtttcgtgaa tttcgcttct ctctgcgctg
     3361 ccttgtgccc gaaaaccacg tagtagtctc ccgccgtcag atcggtgaac aggtacttcc
     3421 cgtttgcatc ggtggctgtc gtgtagatat gcatgaattg atcgtccatc aagttcaccg
     3481 aaacgcccgg aactcctttt tctcctccat cctgaatccc gttctcattt tcgtcttccc
     3541 agaccaggtc cccgatctgg cccgtgccct gcatcgcatg gttataaaag ccggcgtccc
     3601 agttgagctg taccagtgct ccctctttga tgacgatgat gccggttcgg cctgatattc
     3661 cgggatcgct gtccagatca tcgtcatttt ggacgttatc catcgggctg tggatccagt
     3721 tcccctgagg ggggtcaaac ctgaccctgt acttcccttc tacgaggttg tcgaatacgt
     3781 attttccatc ctcttttgtg gtgacccgcc tgggctgtcc gttctcaccg ttgatcgggt
     3841 tgtcattctc atcgagcaat gccacggata cgccggcaag gcctttctca cctgaatcct
     3901 gctttccatt ctggtttctg tcgttccata caaaatcccc tatcgaaccc ttgccctctg
     3961 gctgcggggg tgcttccact gttacttttt tcacgatctc gcaggttaca ccgttactga
     4021 gcgtcacccg gagtttaacg tcataggttt tggccgccgc aaatgtgtgc gtcgggttct
     4081 ggccgaggtc ctgccaccct tttccatccc cgaagtccca ctcccacgta gtcggtgcca
     4141 cgccggcagg gcaaccggtt gtttggacgc tcccctggaa atgatagact ctcaacgaca
     4201 acggatcggg attttgcact ccggcaggag gagctcgatt tgtggtagag aaggtgaaat
     4261 ccgctgtgca ggcacacccg cctcctgcgg ttgaactcgt atagtggctg agatcggttg
     4321 ccgtcaccgg ctttgcggtc tgatcgtctg ccgtgaccgt gacggtgttc ttataggaac
     4381 cgcccggcat gccgctcgtg tcctttgccg tgtcgtcttt cgtgtagacc catgtctcgc
     4441 cggggtcgag tttgccgtca ccgttggcgt cgcccgcggg cggtcctgcg atcgggccgg
     4501 ccttatcgtc cgtcaccttg acgttggcga gcgggcccga tccgggattc gtcacctcgt
     4561 aggtccacgt caccttatcg ccgaccttga tcgtcggccc cggggggttc ttggcaatct
     4621 ggccgttggt ctttttgacg acggcgatct ggcccgctcc cctgaccagc ccggcatccc
     4681 actggagctg cttctcccct gccttgag
//
LOCUS       DAOSII010000136         4688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_16248_length_4688_cov_43.934815, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000136 DAOSII010000000
VERSION     DAOSII010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16248_length_4688_cov_43.934815"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3435
                     /locus_tag="PLV90_08845"
     CDS             <1..3435
                     /locus_tag="PLV90_08845"
                     /inference="COORDINATES: protein motif:HMM:NF023252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3344 domain-containing protein"
                     /protein_id="HOF97259.1"
                     /translation="DKSPTYTFAAGAYDIRLTATNANGNDTLTKTQYISSSAGAKRLA
                     TVQSGTVSGDLYVGAYGGWSSGISYATNTFNQTFALPAYTDIQWARLYTVVYAAGTDN
                     RAGTVTVRFDGNGTYATLGIETLATAGDNTANVYPVNDHVNRQYSDYLLWYDVTDLIG
                     SEDPKAEVVATPVAPNFDGRIKELVLVVAYNDGDGDQVHYWVNEGHDWQANGADGVTS
                     TFATGGLGAGWTDATLRNVMLSSKDALYTFNGAPCTGADSSITFRANTWDVRTNLTAG
                     SDSNFTYVPNGGSYKTTLATLAVKYPASAQPDLTVSTLASNNGEVFSASENTYTATIT
                     NIGTGDAGAFDAGFDVSGVTGTVPVTGLAAGAETTVTWTDETIRNAGDTVTITVTADP
                     DGLITESNEANNIRTIDKTVVHNGYRGMRWTDGGDIATAVTYDIRGDFLWSAGDGAYL
                     SASTYPNWTTYTVNWTGGDLAVPEGATIAGARLYVPYTWGKGPIFPSNVTLAFNSETV
                     DRSAFYEDEKMWGSSYPYGMVVYDVTDQFSAEGNTALLANTFPGGGNVSVRGMLLAVV
                     YDDGTTAPHTVLMNEGFDLLYGGEAQGTTPEQATAYAPFSGVETDDVLSATLITVAPG
                     AGPTKGDLLFNDQTWTRVWNYTGTTQIGVDERDVTTFLIDGENLVGFRSSADYMEAAA
                     AILVLEYPAPPGTIFVTSDPTGAAVWLDGEDTGEVTPTTLEDVPAGDHVVTLKLDGYA
                     DASRPVTVVSDETVTVDLELTTLTGSLEVTSVPDGAAIFIDGADTGEVTNATIDGIGV
                     GTHTVTLKLAGYAGAVAEVTIAENETAPLHLDLIEAHGSITVASMPEGAAIFLDGADT
                     GKLTNALLENVAAGEHTVTVKLAGYIDASRTVTVTDNETITINFLLTEPAGSIVVTSA
                     PDGARIFLDGTDTGEVTNTTLENVPVGTPTVRVERDGYLEAEETVTVTSGEIAAVHFD
                     LEPAAITLQPGWNFVSTPKRLADGQDTIAVFDGVDTNGRPVLFYNGTCQWEDLSREDA
                     FRPLDGIWIYSNSTYEIPLSFAAASYSPPPTKYLDEGWNAIGFTGTVPEPAVTTLRTV
                     EMTWANLWGWKAREQAYDASIIRGATGRYGEMRELSPFQGYWIYMNDADRLTAIEA"
     gene            3432..4505
                     /locus_tag="PLV90_08850"
     CDS             3432..4505
                     /locus_tag="PLV90_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97260.1"
                     /translation="MTMKFQQGMILTLLLALAIGVAAGEDVPPALPHDFWGGVTIDGS
                     PAPAGTEITALIGDTACGSIKTTKIGEYGSSSRHEGGRLVVSGTDGQSGETITFLVNG
                     RTAREMATFTPDSTTRLDLSVVPTPPSGGNGGGGPSGSSPAPVETSAPPAPAMPVGRA
                     SLPLSGTGEVSESVTVGTGDGAGSLTVGEGTRARDANGNPLGEVTIARATAGLPPVPP
                     GATIGFALDCGPAGATFDPPATLTYTLSEEEWGRIGNSATLRVMWYNPGTGAWQEVPA
                     TVDPATRTVTAEVSHFSLFALTWAAASPAEATTGPAGTAPGDVPPGSEQPTGGETPWA
                     LIAAGVLVVAAVLATGWYLLKKR"
BASE COUNT          892 a         1667 c         1415 g          714 t
ORIGIN      
        1 gacaaaagcc cgacctacac ctttgcagcg ggagcctacg acatccggct caccgcgacc
       61 aatgccaacg gaaacgacac tctaacgaag acccagtaca tctcgtcctc cgcaggggca
      121 aaacgcctcg ccacagtgca gagcggcacg gtctccggcg atctctacgt gggggcatac
      181 gggggatgga gctccggtat ctcatatgcg acgaacacgt tcaaccagac gttcgccctc
      241 ccggcataca ccgatatcca gtgggcacgg ctgtacacgg tcgtctacgc cgcaggtacg
      301 gacaaccggg cggggaccgt gaccgtcagg ttcgacggca acggcacata cgccacgctc
      361 ggcatcgaga cccttgccac cgccggggat aatacggcaa acgtctaccc ggtcaacgac
      421 cacgtgaacc gccagtattc ggattacctg ctctggtacg acgtgaccga tctcatcggt
      481 tcagaggacc cgaaagcgga ggtcgtcgca accccggtgg caccgaactt cgacggccgc
      541 atcaaggagc tcgtcctcgt ggtcgcgtac aacgacggcg acggcgatca ggtgcactac
      601 tgggtgaacg aggggcacga ctggcaggca aacggcgccg acggggtgac ctcgaccttt
      661 gcaaccggcg ggcttggtgc cggctggacg gatgcaaccc tccgaaacgt catgctctcc
      721 tcaaaagacg ccctgtatac gttcaacggc gctccctgta cgggtgcgga ctcatcgatc
      781 acgttcaggg cgaacacctg ggacgtcagg accaacctga ccgccggcag cgacagcaat
      841 ttcacgtacg tgccgaacgg cgggtcgtac aagaccacgc tcgcaacact tgccgtgaag
      901 tacccggcct ctgcacagcc cgacctcaca gtcagcaccc tcgcgtcgaa caacggcgag
      961 gtcttctccg catccgagaa cacctacacg gcgacgatca ccaacatcgg caccggtgac
     1021 gcaggtgcgt tcgacgccgg gttcgacgtc agcggtgtga ccggcacggt tcccgtcacg
     1081 ggcctcgcgg ccggcgcgga gaccaccgtc acctggaccg atgagacgat tcgcaacgcc
     1141 ggtgacaccg tgacgatcac cgtcaccgcc gaccccgacg ggctcatcac tgaatcgaac
     1201 gaagcgaaca atatccgcac catcgacaag accgtcgtcc acaacggcta ccgcggcatg
     1261 cgctggaccg acggcgggga catcgccacc gccgtcacct acgacatccg cggcgacttc
     1321 ctctggtcgg ccggcgacgg cgcctacctc tccgccagca cctacccgaa ctggaccacc
     1381 tacaccgtga actggacggg cggcgatctt gccgtccccg aaggcgccac gatcgccggc
     1441 gcccggctct acgtccccta cacctggggc aagggtccga tcttcccctc aaacgtcacc
     1501 ctggccttta acagcgagac cgttgatcgg tctgccttct acgaggatga gaagatgtgg
     1561 ggctcctcgt atccctacgg catggtcgtc tacgacgtca ctgaccagtt ctccgctgag
     1621 ggcaacaccg ccctgctggc aaacaccttc cccggcggcg gcaacgtctc ggtccgtggg
     1681 atgctccttg cggtcgtcta cgacgacggc accaccgccc cccacaccgt cctcatgaac
     1741 gaaggtttcg acctcctcta cggcggtgag gcccagggga ccacgccgga gcaggcaacc
     1801 gcctatgcac cattctccgg cgtcgagaca gatgacgtcc tgagtgccac cctgatcacc
     1861 gtcgcccccg gcgccggacc gaccaagggc gacctcctct tcaatgatca gacctggacc
     1921 cgcgtctgga attacaccgg cacgactcag atcggggttg atgaacgcga cgtgaccacg
     1981 ttcctcatcg acggcgagaa cctggtcggg ttccggagca gcgccgacta catggaggcc
     2041 gccgcggcca tcctggtcct cgagtacccg gctccgccgg gcaccatctt cgtcacctcg
     2101 gaccccaccg gcgcggcggt ctggctcgac ggcgaagata ccggcgaagt cacgcccacc
     2161 acgcttgaag atgtcccggc cggcgaccac gtcgtcaccc tgaagctcga cggctacgcc
     2221 gacgcttcga ggccggtcac cgtcgtcagc gacgagaccg taaccgtcga cctcgaactg
     2281 accaccctga ccggcagcct cgaggtcacc tcggtgcccg acggcgccgc gatcttcatc
     2341 gatggcgcag acaccggtga ggtcaccaat gcgaccatcg atgggatcgg cgtcggcact
     2401 cacaccgtca ccctgaaact cgccggctac gccggcgctg tcgccgaggt caccatcgca
     2461 gagaacgaga ccgcccccct ccaccttgac ctcatcgagg cccacggtag catcaccgtc
     2521 gcctcaatgc ccgaaggcgc cgcaatcttc cttgacggcg ccgacaccgg caaactgacg
     2581 aacgccctcc tggagaacgt cgcggccggc gagcacaccg tcaccgtgaa actcgccggc
     2641 tacatcgatg cctcgaggac cgtcacggtc actgataacg agacgatcac gatcaacttc
     2701 ctcctgaccg aaccggccgg gagcatcgtc gtcacctcgg cgcccgatgg cgcccggatc
     2761 ttcctcgacg gcacagacac cggcgaggta acgaacacca ctctggagaa cgtcccggtc
     2821 ggcaccccca ctgtccgggt ggaacgggac ggctatctgg aggcggagga gaccgtaacc
     2881 gtcacctcgg gcgagatcgc ggccgttcac ttcgatcttg agcccgcggc aatcaccctc
     2941 cagcccggct ggaacttcgt ttcgaccccg aagcggctcg ccgacgggca ggacaccatc
     3001 gcggtcttcg acggcgtgga caccaacggg cgcccggtgc tgttctacaa cggaacctgc
     3061 cagtgggaag acctgagtcg agaggatgcg ttccggccgc tcgacgggat ctggatctac
     3121 tcaaacagca cctacgagat cccgttgtcc ttcgccgccg cttcatactc cccgccgcct
     3181 acgaagtacc tcgatgaggg ctggaacgcg atcgggttta cgggtaccgt ccccgaaccg
     3241 gcggtcacca cgctccggac ggtcgagatg acctgggcga atctctgggg ttggaaggcg
     3301 agagaacagg cctacgatgc ctcgatcatc cggggtgcga ccggcaggta cggtgagatg
     3361 cgggaactct cccccttcca gggctactgg atctacatga acgacgcgga tagactcacc
     3421 gccatcgagg cgtgacgatg aagttccagc aggggatgat cctgacgctc ctcctcgccc
     3481 tcgctatcgg ggtcgcggca ggggaggatg tacctcccgc gctcccccac gatttctggg
     3541 gcggcgtgac gatcgacggt tccccggcac ccgccgggac cgagatcacc gcactgatcg
     3601 gggatacggc atgcggttca atcaagacga caaaaatcgg ggagtacggc agttccagcc
     3661 gacatgaagg gggccgcctg gtggtcagtg ggaccgacgg tcagtcaggg gagacgatca
     3721 ccttcctggt caacggacgg actgcacggg agatggcgac cttcacgccg gatagcacga
     3781 cccgcctgga cctctccgtc gtccccacac ctccatccgg cggaaacggc ggtgggggac
     3841 cgtcggggtc ctccccggcc cctgtcgaga cctccgcccc gcccgccccg gcgatgcctg
     3901 tcgggcgggc gtccctcccc ctctcgggga cgggtgaggt gagcgagtcc gtgacggtcg
     3961 ggaccggcga cggcgccggt tccctgaccg tcggcgaggg gacgcgtgcc cgcgacgcaa
     4021 acgggaaccc actcggcgaa gtgaccatcg cgcgcgccac cgccgggttg cccccggtcc
     4081 cgccgggggc gacgatcggg ttcgccctcg actgcgggcc ggccggcgcc acattcgacc
     4141 ccccggccac cctcacctac acgctctccg aagaggagtg ggggaggatc ggcaattccg
     4201 ccaccctgcg ggtgatgtgg tacaaccccg ggaccggcgc atggcaggag gttcccgcaa
     4261 ccgtggatcc ggcaacccgg accgtgacgg cagaggtctc gcacttcagc ctcttcgccc
     4321 tgacgtgggc cgccgcatct ccggcggagg cgaccaccgg cccggccggc accgcacccg
     4381 gtgatgtccc gcccggttcg gaacagccca caggaggcga gaccccgtgg gcgctcattg
     4441 cggccggagt cctggtcgtc gccgcggttc tggcgacggg gtggtatctc ctcaagaaga
     4501 gatgaccggt cccaccctct ctttttcacc caaaaacggg cggcctcagg tcggtttcgt
     4561 gaaaatagtc cgggcggccc ggagcgcctc agcatcccag ggtatgctga cttccgttcc
     4621 ccttcagaac aggagaaaca ccagcgcgac cgtgatcacc ttcgcggcca tgctcgctgc
     4681 atacgtgg
//
LOCUS       DAOSII010000137         4665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_16335_length_4665_cov_43.865727, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000137 DAOSII010000000
VERSION     DAOSII010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16335_length_4665_cov_43.865727"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2689
                     /locus_tag="PLV90_08855"
     CDS             <1..2689
                     /locus_tag="PLV90_08855"
                     /inference="COORDINATES: protein motif:HMM:NF023252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3344 domain-containing protein"
                     /protein_id="HOF97261.1"
                     /translation="DSGGGEAVDYGYTGKRLDIAASGTLHGNLTYYDFGSYTGLIPAG
                     ESREFAIDIPAPANGTIRYARLYVYTTWGHDEKKKEGTTVRIDLSVDGRAIRADRTYN
                     DRKYEGIYNYLIETHAFNVTGIPGGIAGEHVVKVKNDGRKDDLFAAYGVSLLVVTEDP
                     DEPETTYWIAEGSDAVFASSQVGTTSEDCTTTAVFEDPIDPATTGEARLILVSTAGSG
                     MGNDEHRILFNDGEWFNLLAAGSSAVSVADLDARPFLRQSANEAGIQSLITATKGDYM
                     ENRAIILVVRKGSPPEGYEMNATPTTSGPGTPAVSADTGSRIVLDAANGRIGAMHITA
                     DGGAVDLYIPEGAAIVDGAKDPVSVLVLQRLRPGDAGGVERAYAVGSDDMASDIALTL
                     TISADAPGGAIARYDRSSGTWQPLETRADAAGGTVSAQIRQGGTYAVRHDGHAPAAHA
                     LSGGLLNTLANIPAGGLSGRLLEAVTAFLGLFGIVPAGEVPAMTAAPVPAPTPGPVPA
                     ALEPAPAPDITAMRFALSVLSDPPGALVDIDGEYTGRATPAAFASLPGGNHTVRVHAD
                     GAEPVEETILLDHDDEVFIEFPGAGTSPAGPLRTWEQNHYGGVYVDSFPAGADILVDG
                     QKVNRKTPDIVYGLKEGLHTIKLRKENTAFSSEKEQVWVERNTITRVTFASGNADLVR
                     SITFDSEDCAQKPFSVNGRYLGDRLPKKVDVRGIGGAYITVRDSESYRSYRIPATAGS
                     NDTVTVTLPDAPCSLLVTSVPAGAAIAVDGFLTGFATPYVIENISEGKHRIAVSKPGH
                     IPAEREVLLTDGSGDTTVKVTLEPYVWGSLAVSSTPPDAKIYLFGRDTGEVTPHTFHY
                     LDIGSYPVKVVGKEGSKTIEGVLVSPHAVAGCHADLTGE"
     gene            2879..>4665
                     /locus_tag="PLV90_08860"
     CDS             2879..>4665
                     /locus_tag="PLV90_08860"
                     /inference="COORDINATES: protein motif:HMM:NF023252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3344 domain-containing protein"
                     /protein_id="HOF97262.1"
                     /translation="MHDTTSRGGRRSPWLLFFIAVVLLAAALLPAAASADPHVGDILF
                     DADMADDATQGPASASFAPGARIVAATADCDLTIGGMPNPTGSVVFAREPNTIRVMNI
                     KNNGPGTSPATVMEVRSDDGWTGRVDIPPIESGKNILSVSIEDPTIRNLAGGTVTYTA
                     EIDPDDTVAESDESNNIRVGQPREVKYNGYKGKRYWEGGSDITTVRTYDLYGGIVHSF
                     GDSRYWSGSFGGDDYWKGYTVTWTADDLPLPEGSSVRDAWLYVPYAWDNTNSAPDNVS
                     IEFNGVGVSYVDWYTDVSNFGAYVDHHYGLFTYNVTSLFRKGANNTALFERDRPGKIS
                     PAGFTLAVVYEDPSATRKQIFINEEFDILGADPLGYGTNETEATAYVPFTGMTIDTGN
                     VVRADLTTFVPWGDCGDGTRPGEGNLLFNDELVREWVWDYGPGTSTQVAVDERDVGDR
                     LRPTGNIAAIQGTEANSPTMVAAQVFLVVEYVDDSSGDAPVAAFTAAPMSGLAPLAVT
                     FADASTNSPTAWSWESRPSGTTENWTLFSTDQSPTHTFAAGAYDVQLTATNAGGSDTL
                     TKARCISSSAGPKRLATVQSGTVSGDLYVG"
BASE COUNT          900 a         1487 c         1501 g          777 t
ORIGIN      
        1 cgattccggg ggcggggaag ccgttgacta cggttacacg gggaagaggc tggatatcgc
       61 cgcctcgggc acgctccacg ggaacctgac ctactacgat ttcggcagtt acacggggct
      121 catccctgcc ggcgagagca gggagtttgc gatcgatatc cccgcccctg cgaacggcac
      181 gatccggtac gcacggctgt acgtctacac cacatggggg cacgacgaga agaagaaaga
      241 agggaccacg gtcaggatcg atctctcggt cgacgggagg gcgatcaggg cggacagaac
      301 ctataacgac cgtaaatacg aggggatata caactacctc atcgagaccc acgcgttcaa
      361 cgtcaccggc atcccgggcg gcatcgccgg agaacacgtc gtgaaggtga agaacgacgg
      421 gagaaaggac gatctctttg cggcgtacgg cgtcagcctc ctggtcgtca ccgaagaccc
      481 agacgaaccc gagacgacct actggatcgc ggaggggtcg gatgcggtct ttgccagttc
      541 gcaggtcggg acgacctctg aggactgtac aacgacggcg gtctttgagg acccgatcga
      601 tcctgccact accggcgaag cccgtctcat cctcgtatcc acggccggtt cgggtatggg
      661 caacgacgag caccggatcc tcttcaacga cggcgagtgg ttcaacctgc ttgccgccgg
      721 ttcgtcggcg gtcagcgtgg ccgacctgga tgcgagaccg ttcctccggc agtcggcaaa
      781 cgaggcgggt atccagagcc tcatcaccgc gacgaaagga gactacatgg agaaccgcgc
      841 catcatcctc gtcgtcagga agggcagtcc gccggagggc tacgagatga acgccacgcc
      901 cacgacgagc ggacccggaa ctccggcggt atctgccgat accggatcgc ggatcgtcct
      961 tgacgcggca aacgggcgga tcggggccat gcacatcacc gccgacggcg gggccgtcga
     1021 cctctacatc cccgagggag cggcgatcgt cgatggggcc aaagatcctg tttcggtgct
     1081 ggtgctgcag aggctcaggc ccggggatgc cggaggtgtc gagcgggctt acgctgtcgg
     1141 cagcgatgat atggcgagcg atatcgccct cacgctgacc atatccgcgg atgcaccggg
     1201 aggggcgatc gccaggtacg atcgctcttc gggcacctgg cagcccctgg agacgagggc
     1261 cgatgcggcg ggaggcaccg tctctgcgca gatacggcag ggagggacct atgctgtccg
     1321 gcacgatggg cacgctccgg cagcgcatgc tctctccggc gggctcctca ataccctcgc
     1381 gaacatcccc gccgggggac tctccggcag gctgctcgag gcggtcacgg cgttcctggg
     1441 gctctttggc atcgtccctg ccggggaggt gccggcgatg acggcagccc ctgtgccggc
     1501 acccaccccc ggcccggtgc cggcagccct tgagccggcg ccggcacccg atatcaccgc
     1561 tatgcggttt gctctctcgg tgctctccga tccgccgggc gcgctcgtcg atatcgacgg
     1621 ggagtatacc ggcagggcga caccggcagc atttgcgtcg ctcccgggcg gcaaccatac
     1681 cgtccgggtg cacgcagacg gcgcagaacc ggtggaagag acgatcctgc tcgatcacga
     1741 cgatgaagtc ttcatcgagt tcccgggagc cgggacatcc cctgcgggac cgctccggac
     1801 ctgggaacag aaccactacg gcggcgtcta cgtcgactcg ttcccggcgg gggcggatat
     1861 cctcgtcgac ggccagaagg tgaaccggaa gactcccgat atcgtctacg ggctcaagga
     1921 ggggttgcac acgatcaaac tgcgcaagga gaacaccgcg ttcagctccg agaaagagca
     1981 ggtctgggtc gaaagaaaca ccatcacccg ggtgacgttt gcgagcggca acgcggacct
     2041 ggtgcgctcg atcacgttcg actcagagga ttgcgcgcag aaacccttct ccgtcaacgg
     2101 caggtacctt ggcgaccgtc tcccaaagaa ggtcgacgtc agggggatcg ggggggcgta
     2161 tatcaccgtc cgcgacagcg agagttaccg gagttaccgg atacccgcca ccgccgggtc
     2221 aaacgacacc gtgaccgtca ccctccccga tgcaccctgc agcctcctcg tgacgtcggt
     2281 gccggcggga gcggcgatag cggtcgacgg atttttgaca gggtttgcga cgccatacgt
     2341 catcgagaac atatctgagg gcaaacatcg gatagcggtc tcaaaacccg ggcacatacc
     2401 ggctgagcgg gaggttctgc tgacggacgg ttccggcgat accacggtga aggtcaccct
     2461 cgagccttac gtctgggggt cgcttgcggt ctcgagcaca ccgccggacg cgaagatcta
     2521 cctcttcggg cgggacaccg gcgaagtgac gccccacacc ttccactacc tcgatatagg
     2581 cagttacccg gtgaaagtgg tggggaagga ggggtcgaag acgatcgagg gcgtgctggt
     2641 ctcgccgcat gccgtcgccg ggtgccacgc ggaccttacg ggggagtgaa agttctccct
     2701 tttttatacc ctctcctctc cgcagccgga tacgcaccat ggccgatata cgtgtcctga
     2761 taaccgggga aacccttctc gcggggcgga tgcccggagt gattacaaat attttatata
     2821 atgttatttg atcatatact ctcattcgat tgagggatca agtgtgatta actttaagtt
     2881 gcatgatact acgtcccggg gcggccgccg atcgccctgg ttattgttct tcatcgccgt
     2941 tgtactcctg gcagcggcgc tgctcccggc ggcggcatcc gccgatccgc acgtaggcga
     3001 tattctcttt gatgccgata tggcggacga cgccacgcag gggccggcct ccgcctcttt
     3061 cgcgcccggc gcccgcatcg tggccgctac ggcagactgc gacctcacca tcggggggat
     3121 gccaaacccc accggcagcg tggtgtttgc ccgcgagccc aacaccatcc gggtcatgaa
     3181 catcaagaac aacggcccgg gaacctcccc ggcaacggtg atggaggtcc gctcagacga
     3241 cgggtggacg ggcagggtcg acataccgcc gatcgagtcc ggcaagaaca tcttgagcgt
     3301 ctcgatcgag gatccgacca tccggaacct tgctggcggg acggtgacct acaccgccga
     3361 gatcgatccc gacgacaccg tcgccgagag cgatgagtct aacaacatca gggtcggcca
     3421 gcccagagag gtgaagtaca acggttacaa gggcaaacgc tactgggagg gcggcagcga
     3481 catcaccacc gtccggacct acgaccttta tggcggcatc gtgcactcct tcggcgacag
     3541 ccgttactgg tcggggagtt tcggcggcga cgactactgg aagggttaca cggtgacctg
     3601 gacggcggac gacctgccgc tgcctgaggg ctcgagcgtc cgcgacgcct ggctctacgt
     3661 gccctacgcc tgggacaaca ccaactctgc acccgacaac gtctcgatcg agttcaacgg
     3721 cgtcggggtg tcgtacgtgg actggtacac cgatgtctcg aacttcgggg cgtatgtcga
     3781 ccatcactac gggctgttca cctacaacgt gacgtcgctc ttccggaagg gcgccaacaa
     3841 caccgcgctc tttgagcggg acaggccggg caagatatcg ccggcagggt tcacgcttgc
     3901 ggtggtctac gaggacccct ccgccacccg aaagcagatc ttcatcaacg aggagttcga
     3961 catcctcggc gccgatccgc tcggttacgg cacgaacgag accgaggcta cggcgtacgt
     4021 gccgtttacg gggatgacga tcgataccgg gaacgtcgtc cgcgcagacc tcacgacgtt
     4081 tgttccctgg ggggactgcg gcgacggtac gagacccggc gaggggaacc tcctcttcaa
     4141 cgatgaactg gtccgggaat gggtctggga ctacggcccg ggcacctcca cccaggtggc
     4201 cgtggatgag cgggacgtcg gggaccgcct ccgcccgacg gggaacatcg cggcgatcca
     4261 ggggaccgag gccaattcgc cgacgatggt ggcggcgcag gtcttcctgg tcgtcgaata
     4321 cgtcgacgat tccagcgggg atgcgcctgt tgccgcgttc accgcagccc cgatgagcgg
     4381 gcttgcgccg cttgccgtca ccttcgccga cgcctcgacg aactccccca ccgcatggtc
     4441 gtgggagtcg cggccgtccg ggaccaccga gaactggacg ctcttctcca ccgaccagag
     4501 cccgacccac acctttgcag cgggagccta cgacgtccag ctcaccgcga ccaatgccgg
     4561 cggcagcgat acgctcacga aggcccggtg catctcgtcc tctgcggggc caaaacgcct
     4621 cgccacagtg cagagcggca cggtctccgg cgatctctac gtggg
//
LOCUS       DAOSII010000138         4596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_16614_length_4596_cov_44.390222, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000138 DAOSII010000000
VERSION     DAOSII010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16614_length_4596_cov_44.390222"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PLV90_08865"
     CDS             complement(<1..229)
                     /locus_tag="PLV90_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOF97263.1"
                     /translation="MEQKTTGRGVAGRPGPSEDIDLYSTPDGTRAVGNPVRRMILAAL
                     RERECTFEEIVALAGRAKSTVSVHLQDLAAAG"
     gene            511..1584
                     /locus_tag="PLV90_08870"
     CDS             511..1584
                     /locus_tag="PLV90_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HOF97264.1"
                     /translation="MKSRYWIALSLLASGAALTWLLVATFTPETRAFIGEVGTTALLL
                     ALGFRGAAIFCRVVRIGALCRGLGYTVPFLSLTITQLLSLFAGSLTPGQVGGEPVRIH
                     RLTRSGLAVGDAGAVVVTERVLDLAVLILITLAALLALQHLWGYLAAIILYPVAAFLA
                     LVLALMLGLLLLIRRPSFMKRVMGRITLGVLSRCSRSRRWLRFCPGTEGAGSLTTTID
                     REVEAFSAGFSRFAGAGRRAAGSALLLTAFEWSFYYATASALLIALGLPPSLPESFLF
                     QGVIQMIAALPLIPGSAGIAEIGAATLYRQLVPTYLLGLFVVLWRLITYYLNIPFGLL
                     ATALAAREKKGGEDADRRRTENL"
     gene            1686..2363
                     /locus_tag="PLV90_08875"
     CDS             1686..2363
                     /locus_tag="PLV90_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5612 domain-containing protein"
                     /protein_id="HOF97265.1"
                     /translation="MTNDTDMYALSIITENRAGVLRDVATVMADHQANIQMIQQSIIT
                     TGANTGKAYLYFEFEECENHGELIADLARVPSVVDVTIYPPFSRIFGSRVIIIGGGAQ
                     VAQVALGAVNEADRHNIRGERISVDTIPLVGEQTLALAVDAVARLPRASILVLAGSLM
                     GGEVSRAVDRVRQAGIPVIALKMAGSVPQHADLIVTDPIQAGVFAVMHVSGKAVFDIN
                     RVRGREF"
     gene            2363..2941
                     /locus_tag="PLV90_08880"
     CDS             2363..2941
                     /locus_tag="PLV90_08880"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOF97266.1"
                     /translation="MNSIDMAVRVLLRDGLIVYPTETVYGLGADALSEDAVMRVYEAK
                     NRPPGKPISIAVSDMDMLTAVAVVDDAARAFIRRFLPGPVTVILPAKSCLPEILTGGT
                     GLIGIRWPDHDVAIAIIARFDSPITATSANVSDSSPPTRPGEVSIPNDYLVDGGELPG
                     TPSTVVDLTTRRILRKGAAWEAVEAFLETLQA"
     gene            2938..3864
                     /locus_tag="PLV90_08885"
     CDS             2938..3864
                     /locus_tag="PLV90_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase subunit beta"
                     /protein_id="HOF97267.1"
                     /translation="MRPVRLRDFVEDRDGCIYAVSNYDNNDRIGCILRYAPEPDGERT
                     SLSGRRYRKYDFAESFAWIREHKPEYLDMVHRVPPGDVVRVYKPEEEIGKVAARNARV
                     RRLLAHFSLPAGSFGCTGSLLCGLENAASDIDMVVYGDAWFSAQRQLRHLVGTGVIPV
                     MSGGMWRKVYDKRVPEISFEAFVLHEQRKWNRGEFEGTYFDLLYTRDYGNLDAVPPGA
                     GEVLGRATIEATVTDASLSFDSPAVYAVDHGEISRVLSFTHTYSGQALAGEVIEARGV
                     VEQHGDERWLIVGTTREAKGEHIVSRTLLESV"
     gene            complement(3861..4523)
                     /locus_tag="PLV90_08890"
     CDS             complement(3861..4523)
                     /locus_tag="PLV90_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HOF97268.1"
                     /translation="MDDYIPVSGVIACHICPRRYYFERSEEREESPRYTVCKQVSAHL
                     GELLEREQIWQEVLCVLPDREEDTRELFDECINACRETAWQRPVQTDLTVESDTLGIR
                     GRVDKVFEDGTFAITRSSTAPRAGAYGTDRLRIACYVACLKETLGQAVAGGYVEYVAS
                     GICRFVEPEPRDRREMIRAIRAAKRVVAGEIPPRPLRAPCDGCPHVERCTAGPRRLSD
                     LF"
BASE COUNT          738 a         1490 c         1518 g          850 t
ORIGIN      
        1 cgcctgcagc cgcgagatcc tggaggtgca ccgagaccgt cgacttcgcc cgcccggcga
       61 gggcgacgat ctcctcgaac gtgcactcgc gctcgcggag cgcagcaagg atcatgcgcc
      121 gcaccgggtt cccgaccgcc cgcgtcccgt ccggcgtcga gtagaggtcg atatcctcac
      181 tcggcccggg ccggccggct acccccctgc cggtcgtctt ctgctccatg aaagatgatc
      241 gaaagggtga tatatgaaag ttcgtatagc gtagaatgtt cgtattatta ctaacatttc
      301 tggcgatacc gcagcacggc aagaggcctg aaccgattct gcatccgccc gatcgcggcc
      361 ctcgatggct gccgcagtgc ccctggtgcc ggccagggac cgcccggtgc gggaagtgcg
      421 atcccttctg aggaagatgt aatcgttatg ggcggtatta cctgtcgtgg cactgcgcca
      481 gaagagcggt cggcaggaag atgatcgagc atgaagagcc ggtactggat cgcgctctcg
      541 ctcctcgcga gcggggcggc cctcacgtgg ctcctcgtgg ccaccttcac cccggagacg
      601 cgggccttta tcggagaggt cggcaccacc gcacttcttc tcgccctcgg tttccgtggc
      661 gcggcgatct tctgccgggt ggtgcggatc ggggctctct gtcgcggcct tgggtatacc
      721 gtgccgttct tgagcctcac gatcacccag ctcctctcgc tctttgcggg ctccctcacc
      781 cccggccagg tgggcggcga acccgtccgg atccaccggc tcacgcggtc cgggctcgcc
      841 gtcggggatg ccggcgccgt cgtcgtcaca gaacgggtgc tggatctcgc cgttctcatc
      901 ctcatcaccc ttgcggctct tcttgccctc cagcacctct ggggctacct tgccgccatc
      961 atactctacc cggtggcggc gttcctcgct ctcgtccttg cacttatgct cgggctcctc
     1021 ctcctcatcc gccggccgtc cttcatgaag cgggtgatgg gccgcatcac cctcggggtg
     1081 ctctcccggt gcagcaggag ccggcgctgg ctccggttct gcccgggtac ggagggtgcg
     1141 ggatccctga cgaccacgat cgaccgcgag gtcgaggcct tctccgcagg cttctcgcgg
     1201 tttgcagggg cgggccggcg ggccgccggc agcgcgcttc tcctcaccgc ctttgagtgg
     1261 agtttctact acgccacggc atcggccctg ctcatcgccc tcggtctgcc gccatccctg
     1321 ccggagtcgt tcctctttca aggggtcatc cagatgatcg cagcgctccc gctcattccc
     1381 ggatcggccg gaatcgcgga gatcggcgct gccacgctct atcgccagct tgtgcccacc
     1441 tacctcctcg gcctcttcgt cgtcctctgg cggttgatca cctactacct caacattccc
     1501 ttcggcctcc tcgcgaccgc ccttgccgca cgggagaaga agggcggaga ggatgccgat
     1561 cggcggcgca ccgaaaacct ataaccctta tgagaccaaa atttatcagt tagcgcgagt
     1621 atgacgaaga ttgccctgtt cctgagacga tcggagaata tcgggatctc cggccgggga
     1681 ggaccatgac gaacgatacc gatatgtacg ccctctcgat catcaccgag aacagggccg
     1741 gcgtactgcg ggatgtggcc acggtgatgg cggaccatca ggccaatatc cagatgatcc
     1801 agcagtcgat catcaccacc ggcgcaaaca ccgggaaggc ttacctctac ttcgagttcg
     1861 aggaatgtga gaaccacggg gagctcatcg ccgacctggc gcgggttccg tccgtcgtcg
     1921 acgtcaccat ctatccgccg ttctcgcgga tattcggatc acgggtgatc atcatcggcg
     1981 ggggtgccca ggtggcgcag gtggcgttgg gagccgtgaa cgaggcggac cgccataaca
     2041 tccgcggcga gcggatatcg gtggacacga tccccctcgt cggcgagcag accctcgcgc
     2101 ttgccgtgga tgcggtggcc cggctccccc gggcatccat actggtgctc gccgggtcgc
     2161 tgatgggcgg tgaggtctcg cgggccgttg accgggtccg gcaggcgggc atcccggtga
     2221 tcgccctcaa gatggcggga agcgtcccgc agcatgccga cctgatcgtg accgatccta
     2281 tccaggccgg cgtctttgcc gtgatgcacg tcagcggcaa ggcggtcttt gacatcaatc
     2341 gcgtacgggg tcgtgaattc tgatgaactc catcgatatg gcggtcaggg tactcctgcg
     2401 ggacgggctt atcgtctacc ccaccgagac ggtctacggt cttggggcgg atgcgctctc
     2461 cgaggatgcg gtgatgaggg tgtacgaggc caagaaccgg ccgccgggaa aaccgatatc
     2521 gatcgcggta tcggatatgg atatgctcac cgccgttgcg gtggtggacg acgccgcccg
     2581 ggccttcatc aggcgcttcc tgcccggccc ggtgacggtc atcctcccgg cgaagtcctg
     2641 cctcccggag atcctcaccg gcggcacggg gctcatcggc atacggtggc ccgatcacga
     2701 cgtcgccatc gccatcatcg cgcggttcga ctcgcccatc accgccacca gcgcgaacgt
     2761 ctcggacagt tccccgccga cccggcccgg ggaggtctcc ataccgaacg actacctggt
     2821 cgacggcggg gaactgcccg ggacaccgag cacggtggtg gacctgacca cccggcgcat
     2881 cctgcggaag ggtgcggcat gggaagcggt ggaagcattc ctggagacct tgcaggcatg
     2941 agaccggtac gactccgtga cttcgtcgag gatcgcgacg gctgcatcta tgccgtctcg
     3001 aactacgata acaacgaccg gataggatgc atcctccgct acgccccgga gccggacggg
     3061 gagcggacga gcctctccgg ccgccggtac cgcaaatacg attttgccga gtcttttgcc
     3121 tggatacggg agcataagcc cgaatacctg gatatggtcc accgggtgcc cccgggcgat
     3181 gtggtcaggg tctacaagcc cgaggaggag atcgggaagg tggcggcgag aaacgcccgg
     3241 gtgaggaggc ttcttgccca cttcagcctc ccggcgggct cgttcgggtg cacgggctcg
     3301 ctcctctgcg gcctggagaa cgccgcttcg gatatcgata tggtggtcta cggcgacgcc
     3361 tggttctcag cccagcgcca gctccggcat ctcgtgggga ccggggtgat accggtgatg
     3421 agcggcggga tgtggcggaa ggtctacgat aaacgggtgc cggagatatc gttcgaggcg
     3481 ttcgtcctgc acgagcagcg gaagtggaac cggggggagt ttgaggggac ctacttcgac
     3541 ctcctctaca cccgggacta cgggaacctg gacgcggttc ctcccggcgc gggcgaggtg
     3601 ctcggccggg cgacgatcga ggcgacggtc accgacgcct ccctctcctt tgatagcccg
     3661 gcggtctacg ccgtggacca cggcgagata tcccgggtgc tctctttcac ccacacctac
     3721 tcggggcagg ctcttgccgg ggaggtcatc gaggcccggg gtgtcgtgga gcagcacggc
     3781 gacgagcggt ggctgatcgt cgggacgacc cgcgaggcaa agggagagca tatcgtatcc
     3841 aggacgctgc ttgagagcgt ctagaagagg tcggagagcc tccttggtcc ggcggtgcac
     3901 cgctcgacgt gggggcagcc gtcgcagggg gcccggaggg gccgcggcgg gatctcgccc
     3961 gcgacaaccc gttttgccgc ccggatcgcc ctgatcatct cccgccggtc ccgcggctcc
     4021 ggttcgacga accggcagat accgcttgcg acgtactcga cgtaaccgcc ggcgacggcc
     4081 tggccgaggg tctccttcag gcaggcgacg tagcaggcga tcctgagtcg gtcggtgccg
     4141 taggcgccgg cgcggggcgc ggtgctcgac cgggtgatgg cgaacgtgcc gtcctcgaac
     4201 accttgtcga ccctcccccg gatgccgagg gtgtcggact ctacggtaag atcggtctgg
     4261 acgggccgct gccaggcggt ctcgcggcag gcgttgatgc actcatcgaa gagttcccgg
     4321 gtatcctcct ctcgatcggg gaggacgcag agaacctcct gccagatctg ttctctctcc
     4381 aggagttcgc cgaggtgcgc ggagacctgc ttgcagacgg tgtagcgggg cgactcttcg
     4441 cgctcctccg agcgctcgaa gtagtagcgc cgggggcaga tgtggcaggc gatgacgccg
     4501 gagaccggga tgtaatcgtc catacgcatc ggttgagaga ggtacgtctt tcctctttat
     4561 atgggggtat cttctgtgcc ggcggggggg atgcgg
//
LOCUS       DAOSII010000139         4567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_16728_length_4567_cov_50.752438, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000139 DAOSII010000000
VERSION     DAOSII010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16728_length_4567_cov_50.752438"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(570..1421)
                     /locus_tag="PLV90_08895"
     CDS             complement(570..1421)
                     /locus_tag="PLV90_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HOF97269.1"
                     /translation="MHETFGIPEIIEAIQSLLTDPGVNRLGPDVLEPAWESPLIGVSR
                     GDDPLYAEFQETIGPFHWTPLEAFCTGFPDAGVTASDIAVVVWALPQTAATRRDHRAC
                     TALPSRRWSLARHYGEAVNDRIRDHLARTLTNAGYPAVAPARLPVFRRETSERFGIAS
                     RWSERHAAYASGLGTFGLCDGLITERGKAVRLGSVVAAINLPATPRPYSNRTAYCLHF
                     SGDECDACIRRCPAGAISADGHDKERCFAYIREMMVPFVESDIGIPVTSCGLCQTGVP
                     CEGRRPR"
     gene            complement(1464..3164)
                     /locus_tag="PLV90_08900"
     CDS             complement(1464..3164)
                     /locus_tag="PLV90_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOF97270.1"
                     /translation="MVEGSYACGNSQKPLIGITIGEMLNRIAAAHPDSEAIVSVHQGI
                     RWTYAEFLERVNTLARALMALDVERGDRVAIWALNYAEWVLVQFATAKIGAIMVNINP
                     AYRTYEFEYAMKQSEVQTLLLQGRFKTSDYVGMFYESCPEAFEAKPGRINSDKFPFLK
                     NVVFLGDIPYNGMYTWDDLLARAALISPDELREREESLNFDDAVNIQYTSGTTGFPKG
                     VVLTHHNILNNGFIIGEGMKFTHEDRLCIPVPFYHCFGMVLSNMAAVTHGSAMVLPSP
                     VFSAEAVLATVQDERCTALHGVPTMFIAELSHPDFSKYRLDSLRTGIMAGSPCPTEVM
                     REVNQKMNMSEIVIVYGQTETSPGVTMTTTADPLERRVSTVGRPFPHTEIKIVDPHTK
                     RILPRGETGEICARGYCVMRCYYNNPNATRATIDENGWNHTGDLGTMDEEDYVKIVGR
                     LKDMVIRGGENIYPREIEEYLHNHPSVADVYVIGVPDQKYGEELMAWIKLENGANLTE
                     DEVKEFCRGRIAHFKIPRYVKFVDDFPMTVSGKIMKFKMREAAIAELGLECASQIETA
                     "
     gene            3255..3407
                     /locus_tag="PLV90_08905"
     tRNA            join(3255..3292,3372..3407)
                     /locus_tag="PLV90_08905"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3289..3291,aa:Trp,seq:cca)
     gene            3761..>4567
                     /locus_tag="PLV90_08910"
     CDS             3761..>4567
                     /locus_tag="PLV90_08910"
                     /EC_number="4.1.2.43"
                     /EC_number="4.2.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,6,7,8-tetrahydromethanopterin
                     hydro-lyase/3-hexulose-6-phosphate synthase"
                     /protein_id="HOF97271.1"
                     /translation="MYLIGEALVGDGAELAHIDLVMGNKEGAVGQAFANSISQLSRGH
                     TPLLAVIRPNLPTKPSTLVIPKVTLKKEYQVNQMFGPVQAAVAKAVADSVEEGVFEGL
                     DIEDIVIMASVFVHPTAQDYNKIYRFNYGAMKLALRRALDRFPDVDTLLHEKDRAAHA
                     VMGFKVQRLWDPPYLQVAMDLVDKNHMRKVLESLPQNDHLIIEAGTPLIKKFGLSIIS
                     EIREIRPSAFIVADLKTLDTGNLEARMTADAGADAVVISGLAPLSTIEKAI"
BASE COUNT          836 a         1408 c         1447 g          876 t
ORIGIN      
        1 cgggcgtcat tggtcccggt ccgggcggga agagttgtgc tgaggctgcg cctgtagcaa
       61 gcacgatggc gatgaccaga acagcagcaa ctgccattgt acgtatgaat cgtttcatac
      121 cgatacctcc tgataacctg caaacctcgc cggccctcgt gtgggggcga ggctccggca
      181 tgagggcgta tgcgtccggg tttcatcccg gcagagaacg gtgagatcga tgatacggcc
      241 ggcagagggg cgctacgccg cgccccaaaa gaccggtgag cagggtggat acggtggaga
      301 aggactcacg gcagggtaca tatcctgacc catcaccccc acgctgcagc ccgatggcga
      361 tcccacaata tataggttgg cccgggccgg caccgggcca tctttctacc attgcacccg
      421 atccatcgcg atcggcgatg atcccgggcg acatcgtgac gtctccggta gagccggcct
      481 gcaccatcct tcttcaccgc gccggcacgg tcatgaccgc cctctgccag ggccgggaga
      541 cgaagaggcc accgcggcag aataccccat tatcgcggcc gccgcccctc gcagggcacg
      601 ccggtctggc agaggccgca gctcgtgacc gggatgccga tatcgctctc gacgaagggc
      661 accatcatct cccgaatgta cgcaaagcat cgctccttgt cgtggccgtc cgccgagatc
      721 gcgcctgcgg ggcaacggcg gatgcaggcg tcgcactcat ctccggagaa gtggaggcag
      781 tatgcggtcc tgttgctata cggccgcggc gttgcgggaa gatttatcgc ggccacgacc
      841 gacccgaggc gcaccgcctt gccccgctcc gtgatcaggc cgtcgcagag gccgaaggtg
      901 ccgaggcccg aggcataggc ggcgtgccgc tccgaccagc gcgaggcgat gccgaaccgc
      961 tcagaggtct ctcgccggaa caccgggagc ctggccgggg cgacggccgg gtatccggca
     1021 ttggtgagcg tccgggcgag gtggtcgcgg atgcggtcgt tcaccgcttc tccgtagtgg
     1081 cgggcaaggc tccagcgccg cgagggaaga gccgtgcatg cacggtggtc gcgccgcgtt
     1141 gcggcggtct gcggcaacgc ccagacgacg acggcgatat cggatgccgt gacgccggca
     1201 tccgggaacc cggtgcagaa cgcctcgagg ggcgtccagt gaaacggccc gatcgtctcc
     1261 tggaactcag cgtacagcgg gtcatcgccg cgcgagacgc cgatgagcgg gctctcccag
     1321 gcaggctcca gaacatccgg gcccaggcgg tttacgcccg ggtcggtgag gagcgattgg
     1381 atcgcctcga taatctccgg gatgccgaag gtctcatgca tacgaacacc acgtcgtgcg
     1441 cggtaaaaaa gttggggagg gcgttatgcg gtctcgatct gagacgcgca ctcaaggccg
     1501 agctccgcga tcgccgcctc ccgcatcttg aacttcatga tcttgcccga gaccgtcatg
     1561 gggaaatcat cgacgaactt gacgtagcgc ggtatcttga agtgcgcgat ccggccccgg
     1621 cagaactcct tcacctcgtc ctcggtgagg ttcgccccgt tctccagctt gatccaggcc
     1681 atcaactcct cgccgtactt ctggtccggt accccgatca cgtagacgtc ggcgaccgac
     1741 gggtgattgt ggaggtactc ctcgatctcg cgcgggtaga tgttctcgcc gccccggatc
     1801 accatatcct tcaggcgccc gacgatcttg acgtagtcct cctcgtccat cgtcccgagg
     1861 tcgccggtat ggttccagcc gttctcgtcg atcgtcgcac gggtggcgtt cggattgttg
     1921 tagtagcacc gcatcacgca gtatccccgt gcgcagatct cgccggtctc cccgcggggg
     1981 aggatcctct ttgtgtgcgg gtcgacgatc ttgatctcgg tgtgggggaa cggcctcccg
     2041 acggtggaga cgcggcgttc caggggatcg gcggtcgtgg tcatcgtgac gccgggagag
     2101 gtctccgtct gcccgtagac gatcacgatc tcggacatgt tcatcttctg gttgacctcc
     2161 cgcatcacct cggtcgggca gggcgacccg gccatgatgc cggtgcggag cgagtccagc
     2221 cggtacttcg agaagtccgg gtgagagagt tcggcgatga acatcgtcgg caccccgtgg
     2281 agcgccgtgc accgctcatc ctgtaccgtg gcgaggaccg cttcggcgct gaagaccggc
     2341 gagggcagga ccatcgcgga gccgtgggtg acggcggcca tgttggagag gaccatgccg
     2401 aaacagtggt aaaagggcac cgggatgcag agccggtcct cgtgggtgaa cttcatcccc
     2461 tcgccgatga tgaacccgtt gttcagtatg ttgtggtggg tcaggacgac gcccttggga
     2521 aagccggtgg tcccgctggt gtactggatg ttgaccgcgt cgtcgaagtt aagcgactct
     2581 tcccgttccc ggagctcatc ggggctgatg agcgcggccc tggcaaggag gtcgtcccag
     2641 gtatacatgc cgttgtaggg tatatcgcca aggaagacga cgttctttag gaacgggaat
     2701 ttgtcgctgt tgatccggcc cggctttgcc tcgaacgcct cggggcagga ttcatagaac
     2761 atgccgacgt agtcggacgt cttgaaccgc ccctggagga ggagcgtctg cacctcggac
     2821 tgcttcatgg cgtactcgaa ctcgtaggtc cggtaggccg ggttgatgtt caccatgatg
     2881 gcgccgatct ttgcggtggc gaactggacc agcacccatt ccgcgtagtt cagcgcccat
     2941 atggcgacac ggtccccgcg ctccacgtcg agcgccatga gggcgcgcgc aagggtgttg
     3001 acgcgctcga gaaactcggc atacgtccac cggataccct ggtggacgga gacgattgct
     3061 tcactgtccg ggtgcgccgc tgcaattcgg ttcagcatct cgccgatggt gatgccgatc
     3121 aggggttttt gcgagttccc acacgcgtaa ctgccctcaa ccattctata taaatgggat
     3181 gcggtacagt tatatcgtta ctggtttctc tcagcgcaat ccttatactg catggcgtcg
     3241 actgtataga gagcggggtc gtggccaagc ccggaatggc gacgggctcc agcggtcttt
     3301 gcacgtgatg aacaatgggt ctcctgattg agagatgatg acccgttgga gcactgatgc
     3361 atgtcggaga gacccgtcga tcgtgagttc aaatctcacc gaccccacat tctcatgact
     3421 tctcccggat actgccgggt atcgtgttga gccttttgtg ttcccggcta tcttctctcg
     3481 cgatcgaagc ccggtagacc tggtgccggc gctctccagg cgtgagtcgt cgcctgcgtt
     3541 gtgtgctgca ggaggacggt gggtcaaact tgcaatcgcg atcggagttt tcaggcgcaa
     3601 agcgtctgcc cgcgacaccg caccgcttcc ggggcaaaat ccctcttgat caccggctct
     3661 ccctgaaaaa acctgtatcg ctcgcgattt ctacggattg gcccggatga tctttaatac
     3721 ctgtctcacc aatatttctg cactatctga gggcatgagc atgtatctca tcggtgaagc
     3781 attggttgga gacggcgcag aacttgcgca tatcgatctc gtcatgggaa ataaagaggg
     3841 cgcggtaggg caggcgttcg cgaactcgat atcgcagcta tcgaggggcc acacaccgct
     3901 ccttgcggtc atcaggccga acctgccgac aaagccctcg acgcttgtca tcccgaaggt
     3961 cactctgaag aaggagtacc aggtgaacca gatgttcggg cccgtgcagg ccgcggtggc
     4021 gaaggccgtc gccgactcgg tcgaggaggg cgtctttgag gggctcgaca tcgaggatat
     4081 cgtcatcatg gcgagcgtct tcgtccaccc gaccgcacag gactacaaca agatctaccg
     4141 gttcaactac ggcgcgatga agctcgctct ccgccgggct ctcgaccggt tccccgacgt
     4201 cgatacgctc ctgcacgaga aggaccgggc cgcccacgcg gtcatggggt tcaaggtcca
     4261 gcgcctctgg gatccgccgt accttcaggt cgccatggac ctcgtggaca aaaaccacat
     4321 gcggaaggtc cttgagtcgc tgccccagaa cgaccacctg atcatcgagg cgggcacgcc
     4381 cctgatcaag aagttcgggc tctcgatcat atccgagatc cgcgagatcc ggccgagcgc
     4441 gttcatcgtc gcggacttaa aaacgctcga caccggcaac ctcgaggccc ggatgaccgc
     4501 cgacgccggc gccgatgccg tcgtcatctc cggccttgca cccctctcga cgatcgagaa
     4561 ggcgatc
//
LOCUS       DAOSII010000140         4563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_16760_length_4563_cov_51.368900, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000140 DAOSII010000000
VERSION     DAOSII010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16760_length_4563_cov_51.368900"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..1334
                     /locus_tag="PLV90_08915"
     CDS             123..1334
                     /locus_tag="PLV90_08915"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOF97272.1"
                     /translation="MQKAIPRRKKTVRDIDVTGKRVLVRADFNVPLGEDGAIEDDTRI
                     RASLPTIRYLSDRDARVILCSHLDRPQGVVVERLRLALVANRLSVLLGRPVTALRDCI
                     GPEVESAVAAMSGGDVVLLENLRFHPEEKTDDPAFAEELASLADIYVNDAFGASHRAH
                     ASIVGVPEHLPAVAGLLLAKEVDTFTRILRDPERPFAAVIGGAKVSDKLGVLENIMPR
                     VNLLIIGGGMAATFLASRGYGTGRSHVETDRLDDVRQLSAKAMELGVRLLLPRDAVVT
                     ERLEAGAPTQVVPVAKIPDDWVIADIGPETAGEFCRELEGCRTAVWNGPMGVFEIPEF
                     AEGTRRIAATLANLSGTTVVGGGSTADAVARFGLADKMTHVSTGGGAALTVLAGKSLP
                     GVETLEDAEGS"
     gene            1331..2302
                     /locus_tag="PLV90_08920"
     CDS             1331..2302
                     /locus_tag="PLV90_08920"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOF97273.1"
                     /translation="MKRIGILTSGGDAPGMNACIRAAVRAALANNLTVVGIRRGYTGL
                     IATDTVPLDRAAIRNIIHQGGTILETSRSPDLYTREGRLRVAEAIGKMGLDGVILIGG
                     DGTFHGASLVAADADTAPLVGVPGSIDNDVYGTDYCIGFDTAANCAVEAIDRIRDTAR
                     SHERLFFLEVMGRTAGFLALESGIAGGAEELIIPEEETSIAPMSERIRKGFDIGKKSA
                     IVVVAEAKTPGVSFQIAGEVKKHLDLDSRVVILGHLQRGGPPTLRDRVLGSSLGVAAV
                     EALLEGRSECMVGEVGGALAYTPLEETWKKKKPLNENLRRIFSILSD"
     gene            2370..3341
                     /locus_tag="PLV90_08925"
     CDS             2370..3341
                     /locus_tag="PLV90_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF97274.1"
                     /translation="MDAIEVIGLTKRFGDNVAVKGIDFAVKMGETFGFLGPNGAGKTT
                     TIRMLTGMTKITSGRALIHGHDIARDTLAAKRLMGVVPETSNIYDELSAWDNLIFTGR
                     LYHLKKAEIERNARDLLDTFGLYERCHEPAGGFSKGMKRRLTLAMGLVNNPGVLFLDE
                     PTSGLDVQSRRLIRDVVQDLARKNVTIFLTTHDIEEANVSCDRVAIINRGTIAAIDAP
                     EKLKQTFESLQSIEISFDAVRPGLLEALRTLPQVNDVRKEGDKFRLYTADPPEVLSSL
                     CTFTQEQNMRPISVTTRGPSLEEVFIRLTGLSVGTKGEVRETDAGMV"
     gene            3325..4077
                     /locus_tag="PLV90_08930"
     CDS             3325..4077
                     /locus_tag="PLV90_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF97275.1"
                     /translation="MPGWFEGVSRDIGAAWAIARKDMKIYYIKPSILVSSLLFPFVMF
                     LAFAVGREAAAGTLIPGLLAVTLLFSASSIEPVSIPIERRTKTFDRLLSAPISIRAIV
                     LGASLSGALFASAIGVATTVIALIVLNAGVMNVAALLLGFALTSLCFSTMGTLFAAYR
                     TENVGEVMSLLNLVRLPLLFISGVFIPLASMPGWGQSIAVFSPLTYAHDLMLFAFDGT
                     TLFGPVIDVAALVLAIVIFQAAGIFLYQRSRE"
     gene            complement(4096..4518)
                     /locus_tag="PLV90_08935"
     CDS             complement(4096..4518)
                     /locus_tag="PLV90_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOF97276.1"
                     /translation="MATPRLMEYFNRQPRIGILSTANRDGKVDAAVFGSPQMTDEKTV
                     VMALGENRTFEYLRENPSAVFTIVESGKTIADWKGLRVYLWMKEYATSGEMLETYKKE
                     VARAVGDEAAKMVHAVVTFEITEVRPLVDMGQGWEGSL"
BASE COUNT          823 a         1483 c         1461 g          796 t
ORIGIN      
        1 aacagtatat atgccggcgc atgcatgcag gggagcaccg tgattctaca gggaacggcc
       61 gaaaaacgga ccgggaggcg gaaccgcccg gcccctttcc gctgttccgg aggtgtgccg
      121 gtatgcagaa ggcgatacca agaaggaaga agaccgtccg ggatatcgac gtgacaggaa
      181 aacgagtcct cgtccgcgcc gacttcaacg tgccgctcgg cgaggacggc gccatcgaag
      241 acgacacccg tatccgggca agcctgccga cgatacgcta cctcagcgat cgggatgcgc
      301 gggtcatcct ctgctcccac ctggaccggc cgcagggcgt ggtcgtggag cgtctacgtc
      361 tcgccctcgt ggcgaaccgc ctctccgtgc tcctcggccg gccggtcacc gcgctccggg
      421 attgcatcgg ccccgaagtg gagagtgcag tggccgcgat gagcggcggg gatgtcgtcc
      481 tcctcgagaa cctcaggttc cacccggagg agaagacgga cgatccggcc ttcgcggagg
      541 aactcgccag cctcgcggat atctacgtca acgacgcctt cggggcctcc caccgggcac
      601 acgcgtcgat cgtgggtgtc ccggagcacc tgccggcggt ggccgggctt ttgctcgcaa
      661 aagaggtgga tacgttcacg cgcatcctga gagatcccga acgaccgttt gctgccgtca
      721 tcggcggggc caaggtgagc gacaaactgg gtgtcctcga gaacatcatg ccgagggtaa
      781 acctcctcat catcggcggc ggcatggcgg caacgttcct tgcgagccgc ggctacggga
      841 ccggcaggtc gcacgtcgag accgaccgcc tggacgacgt ccggcagctc tccgcaaagg
      901 ccatggaact cggcgtccgc ctcctcctgc cccgggatgc cgtcgtcacg gagaggctcg
      961 aggccggtgc cccgacacag gttgtgcctg tcgcaaagat accggacgat tgggtcatcg
     1021 ccgatatcgg acccgagacg gcgggcgagt tctgccgcga actggaaggc tgccggaccg
     1081 ccgtctggaa cggcccgatg ggggtctttg agatccccga gtttgcggaa ggaacgcgcc
     1141 ggattgccgc gaccctcgcc aacctctccg gcaccaccgt cgtcggcggc ggttcgaccg
     1201 cggacgcagt ggcacggttc gggcttgccg ataagatgac ccatgtctcg acgggcggcg
     1261 gggcggcgct caccgtgctc gcgggaaagt cgcttccggg cgtggaaacg ctcgaggatg
     1321 cggaaggatc atgaagcgga tcggcatcct gacaagcggc ggcgacgccc cgggcatgaa
     1381 cgcctgcatc cgggcagcgg tgcgggcggc gcttgcaaac aacctcaccg tcgtcggtat
     1441 acgccggggc tacaccggtc tcatcgccac cgatacggtg ccgctcgatc gggcggcgat
     1501 ccggaacatc atccaccagg gcggcaccat cctcgagacc tcgcggagtc ccgatctcta
     1561 cacgcgggag gggaggctcc gggtggcgga agccatcggg aagatggggc ttgacggcgt
     1621 gatcctgatc gggggcgacg ggacgttcca cggcgcaagc ctggttgccg ccgacgcgga
     1681 tacggcgccg ctcgtggggg tgccggggag catcgacaac gacgtctacg ggaccgatta
     1741 ctgcatcggg ttcgatacgg cggcaaattg tgcggttgag gcgatcgacc gcatccgcga
     1801 cacggcccgg tcccacgagc gcctcttctt ccttgaggtt atggggcgga cggccggttt
     1861 cctggcactc gagagcggca tcgcaggcgg cgccgaggaa ctgatcatac cggaagaaga
     1921 gacgtcgatc gccccgatga gcgagcgcat acgcaaaggg ttcgatatcg ggaagaagag
     1981 cgccatcgtg gtggtcgcag aggcaaagac gccgggtgtc tccttccaga tagccgggga
     2041 ggtcaaaaaa cacctggatc tcgattcccg ggtcgttatc ctcggccacc tccagcgggg
     2101 cgggccgccg acgcttcgcg accgggtgct cgggagtagc ctcggcgtcg ccgccgtcga
     2161 ggcgctgctt gaggggcgga gcgagtgcat ggtaggggag gtcgggggcg ccctcgcgta
     2221 tacgccgctt gaagagacct ggaagaagaa aaaaccactc aacgagaacc tgcggcggat
     2281 attctccatt ctctctgact aaggcgggag cagaggtact tcagggcctt ttttatcctc
     2341 cggaaagaaa catatggtga gctggggcca tggacgcgat cgaggtgatc ggactcacca
     2401 aacggttcgg tgacaacgtc gccgtgaaag gcatcgattt tgcggtgaag atgggcgaga
     2461 cgttcgggtt tctcggcccg aacggggcgg gcaagaccac caccatcagg atgctcaccg
     2521 gcatgaccaa gatcacgagc ggtcgtgccc tgatccacgg ccacgacatc gcccgcgata
     2581 ccctggccgc gaaacgcctg atgggcgtcg tccccgagac gtcgaacatc tacgacgagc
     2641 tctccgcctg ggacaacctc atcttcaccg gccggctcta tcatctcaag aaggccgaga
     2701 tcgagaggaa cgcccgggac ctcctcgata ccttcggcct gtacgagcgg tgccacgagc
     2761 ccgcgggagg gttctcgaag gggatgaaac gccgcctgac gcttgccatg gggctcgtga
     2821 acaatccggg agtcctcttc ctcgacgagc ccacgtcagg gctcgatgtc cagagccggc
     2881 ggctcatcag ggacgtcgtc caggaccttg cccggaagaa cgtcaccatc ttcctgacga
     2941 cgcatgacat cgaggaggcg aacgtctcct gcgaccgggt cgccatcatc aaccggggaa
     3001 cgatcgcggc gatcgatgcg ccggagaagt tgaagcagac cttcgagagc cttcagtcga
     3061 tcgagatatc cttcgatgcc gtccggcccg gccttcttga agcactcagg acgctcccgc
     3121 aggtcaacga cgtcaggaag gagggggaca aattccggct ctacaccgcg gaccccccgg
     3181 aggtcctgag ctctctgtgc acgtttacac aggagcagaa tatgcgccct atcagcgtca
     3241 ccacccgcgg gccgagcctg gaggaggtct tcatccgcct caccgggctc tccgtcggca
     3301 cgaaggggga ggtgagagag accgatgccg ggatggtttg agggagtctc ccgtgacatc
     3361 ggcgccgcat gggcgattgc gcgcaaggac atgaagatct actacatcaa gccctccatc
     3421 ctggtatcca gcctgctctt cccgttcgtc atgttcctcg cgttcgccgt agggagggag
     3481 gccgcggccg gaaccctgat ccccggtctc ctcgccgtca ccctcctctt cagcgcctcc
     3541 tcgatcgaac cggtctccat accgatcgag cggaggacga agacgttcga ccgcctcctc
     3601 tccgccccga tatcgatccg ggcgatcgtc ctcggggcaa gcctgagcgg cgccctcttc
     3661 gcatcggcga tcggcgtcgc gacgaccgtc atcgccctga tcgtcctcaa cgccggggtc
     3721 atgaacgtcg cggcgctcct gcttgggttc gcgctcacct ccctctgctt ctcgacgatg
     3781 ggcaccctct ttgccgccta ccgcacggag aacgtgggcg aggtgatgtc gctcttgaac
     3841 ctcgtccgcc tgcccctcct cttcatcagc ggggtcttca tccccctcgc gagcatgccg
     3901 ggatgggggc agagcattgc ggtcttctcg cccctcacct acgcgcacga cctgatgctc
     3961 tttgcgttcg acgggacgac cctctttggc ccggtcatcg atgtcgccgc cctggtgctg
     4021 gcgatcgtca tcttccaggc ggccgggata ttcctctacc agaggtcgcg ggagtagacc
     4081 gtgcgggttg ccccgtcaga gggaaccctc ccagccctgg cccatatcga cgaggggacg
     4141 gacttcggtg atctcgaacg tcaccacggc atggaccatc ttcgccgcct catcgcccac
     4201 ggcccgggca acctctttct tataggtctc cagcatctcc cccgacgttg catactcctt
     4261 catccacagg tagaccctga gccctttcca atccgcgatc gtctttccgc tctccacgat
     4321 ggtgaagacg gcactcgggt tctcccggag gtattcgaac gtgcggttct caccgagggc
     4381 catgacgacg gtcttctcgt cggtcatctg cggcgagccg aagacggccg catcgacctt
     4441 tccgtctctg ttcgcggtgc tgaggatgcc gatccgcggc tgcctgttga aatactccat
     4501 aagtctgggt gttgccatga acctgcctcg ccgccccggc ataaaagcgt atggttgtcc
     4561 cgg
//
LOCUS       DAOSII010000141         4559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_16777_length_4559_cov_38.475577, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000141 DAOSII010000000
VERSION     DAOSII010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16777_length_4559_cov_38.475577"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2281)
                     /locus_tag="PLV90_08940"
     CDS             complement(<1..2281)
                     /locus_tag="PLV90_08940"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOF97277.1"
                     /translation="MDALRAYAGVPGLLRKVIDENDGDAWAEITGKIDYIYTHIGYAL
                     RALDRETGFIGEVQSQVRSGKKLLFKPNLVGPQVIDPVTHGEDLGAPICTDWSVMAAL
                     MRWFHDNLDIDYHQMALGEASTSSLLLESVFGRQAGRSITSEAIFEGRSGDFYGGWGF
                     YFVRRYLAERHSPSHTDNPMRGYEESVAGRYFPPGRAGDRLMVYDLNKLCDDLSRGRT
                     VPVPGGANFQEITLHKAIVGGDPRDADDRGDYPGCVLVNVPKMKIHAQDLITNAVKNL
                     GIGLYPTQCPAYTGETSWKYALPSSATPSYKAKLPHMPWVAEVDTASNLPVKDENGDY
                     VVTKTMGMPGTQADVIRAVQNQQVFMVHVSDAIDMINLNHNPEGIAVRIPEGYIWSSL
                     DCVALDHLCARYCFKTVPMAEGLKLKEENGWATEFVHHVPVAKVEGRNIVTAEGLDSP
                     LFRYNLYRYAEERGVGRQQYYVTGWDGITGTPLASLLGHPGRIEDAAFVELMTKTMYY
                     NPTCMLWDMQKTLLCYAEAHDRLTGSSILEQFMEGFDENRDGIIDYDENGQKGFWTLG
                     FSILSHALDLEMTGDYGMLEGRFYQVANLSLKHTDRDWNPQGHDFAREYMLVWIATQA
                     YDMSKAETVSDDPFVPGMQWGGGMWPSWDLAAWHLLSGLVYGGTSPDQVGPGSLYGTA
                     FRYADKTLHNGAYTGSVDQGVSDPRAVATYFRAVSNGADPLDFILYVPAGFGSLAGTK
                     IPNVEETNDPGRIFTAHFAG"
     gene            complement(2654..3268)
                     /locus_tag="PLV90_08945"
     CDS             complement(2654..3268)
                     /locus_tag="PLV90_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97278.1"
                     /translation="MTPNASTATGGTGLFGEASLPLSIALIIPVGVLLVLLGLLLFPV
                     NQGLIPFSPDGQLGLLLVIMAIQMLALGETPVGQYRRSWLLIGIGIVFAALGIFSCIV
                     PGILTDAIRVLLAFLNIAGGAILLGKRFLPMLQGTGSAAELAHLPPIYKRLLGTLTVL
                     NVVAIAFGISMLLPGLVPGMVIAGIVVINGLLLLSLASILIKIG"
     gene            complement(3265..3855)
                     /locus_tag="PLV90_08950"
     CDS             complement(3265..3855)
                     /locus_tag="PLV90_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97279.1"
                     /translation="MRAESELSLEVVILIVFGVFMLLFGLLLFRIQTGELPYAPDSTY
                     GLFLVIVSFQVITMGKTPFGDLRRSWAVIVLGVCTAIVGMVACFIPGLLADPVRILAG
                     ILLSAGGVALLVQLFFSEDKARVWLKVPGALQHLTVACGLVYVVSIVLGIITLLPGIT
                     TDTVTAVFLVIYGVGFFYLSWSIQKAGRLYPPGEAE"
     gene            4216..>4559
                     /locus_tag="PLV90_08955"
     CDS             4216..>4559
                     /locus_tag="PLV90_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HOF97280.1"
                     /translation="MFRPQDDFTYLMPVHFGGGKFDPGTLITQKATGLAVSYETERDL
                     LENYIPEGFELLAPEVQVAFNKFTEINWMHGGQYNLINVSAPVRFHGKKDELDGAYTL
                     VVWENKTAPILGG"
BASE COUNT          949 a         1357 c         1345 g          908 t
ORIGIN      
        1 ccccggcaaa atgggccgtg aatatcctgc ccggatcgtt cgtctcctcc acgttgggga
       61 tcttcgtacc cgcgagactc ccgaaccccg ccggcacata gagaatgaag tccaggggat
      121 ccgctccgtt tgaaacagcc ctgaaatatg tggcgacggc gcggggatca gagaccccct
      181 gatcgacgct tccggtatat gcaccgttat gcagggtctt atcggcgtac ctgaacgccg
      241 tcccgtagag ggaaccagga ccgacctggt ccggagacgt tccaccgtac acgagcccgg
      301 acagcaggtg ccatgccgcg agatcccagc tcggccacat gccccctccc cactgcatcc
      361 ccggcacaaa ggggtcgtca ctcaccgtct cggccttcga catatcgtag gcctgcgtcg
      421 cgatccacac cagcatgtac tcacgggcga aatcgtggcc ttgcggattc cagtctctat
      481 cggtatgctt cagggagagg tttgctacct ggtagaatct cccctcgagc ataccatagt
      541 ctccggtcat ctcgagatcc agcgcatgcg acaggatgct gaaccccagt gtccagaacc
      601 ctttctgtcc gttctcgtcg taatcgatga tgccatcgcg gttctcgtcg aacccctcca
      661 tgaactgttc aaggatcgag gagccggtca ggcgatcgtg cgcctctgcg tagcagagga
      721 gcgtcttctg catgtcccac agcatgcatg tcgggttgta gtacatcgtc ttcgtcatga
      781 gttcgacgaa ggccgcatcc tcaatcctcc caggatgccc gaggagagat gcgagcggcg
      841 tcccggtgat gccgtcccac ccggtgacgt agtactgttg ccggcccacc ccccgttctt
      901 cggcgtaccg gtagaggttg tacctgaaga gaggcgagtc gagcccttct gcggtcacga
      961 tatttcgccc ttcgaccttt gctaccggga catgatggac gaactccgtg gcccagccgt
     1021 tctcctcctt gagtttgagc ccttcggcca tggggacggt cttgaaacag taccgtgcgc
     1081 agagatgatc cagtgcaacg caatccagag acgaccagat gtatccttcg ggaatcctga
     1141 cggcgatgcc ttcggggttg tggttgaggt tgatcatgtc gatcgcatcg gagacgtgca
     1201 ccatgaagac ctgctggttc tgcacggccc tgatgacgtc tgcctgggtc cccggcatcc
     1261 ccatggtctt cgtcacgacg taatctccgt tctcatcctt caccggcagg ttggaggcgg
     1321 tatccacctc ggcgacccag ggcatatgcg gcagtttcgc cttgtagctc ggcgttgcag
     1381 aggagggcag cgcgtatttc cacgaagtct ctcccgtgta tgcggggcat tgcgtcgggt
     1441 agaggccgat gcccaggttc ttcaccgcgt tggtgatgag atcctgcgcg tggatcttca
     1501 tcttcgggac gttgaccagg acgcagccgg gatagtcccc gcgatcgtcg gcgtcacggg
     1561 ggtcgccgcc cacgatcgct ttgtgcaggg tgatctcctg gaagttcgcc ccgccgggaa
     1621 ccggaaccgt ccttccccgt gacagatcgt cgcacagctt gttcaggtcg tagaccatca
     1681 accggtcccc cgccctgccc ggcgggaagt acctgccggc cacgctctct tcataccccc
     1741 tcatggggtt gtcggtgtgg gaaggggagt ggcgctctgc gaggtagcgg cgaacgaagt
     1801 agaatcccca cccgccgtag aaatccccgc ttcgcccctc gaagatcgcc tccgaggtga
     1861 tgctccgccc ggcctgccgg ccaaaaacgc tctccaggag gagcgaagac gtggaggctt
     1921 ccccaagcgc catctggtgg tagtcgatat cgaggttgtc gtgaaaccac cgcatcaggg
     1981 cggccatcac cgaccagtcg gtgcagattg gagcgccaag gtcttcgccg tgtgtcacgg
     2041 gatctataac ctgcggtccg accaggttgg gcttgaagag cagtttcttg ccggatcgca
     2101 cctgagactg cacctcgccg ataaaaccgg tctcccggtc gagagcgcgc agggcataac
     2161 cgatatgcgt gtagatgtag tcgatcttcc ccgtaatttc tgcccaggcg tcaccgtcgt
     2221 tctcatcgat gaccttccgg agcagtccgg gaacaccggc ataggcccgt aaggcatcca
     2281 tccttgtgcc gcagacagga gatccatcgg aatcggtctt cgttctcccg atatccgatc
     2341 ttaccggggt tcctttcagg gaacgttcga gtgtggtcat gatcctctca atcctctcag
     2401 tctgaatggg ggcaatcaaa tcgctctgaa caacatcaga atctcccgta gctccgcgcg
     2461 aggccttcac cttcgatgtc ggaccctatt tgatgattcc ggatgattca gcgtacactg
     2521 cgtaccatta tatgccttct gattcctttc cggtcaccaa gatgcccacc gggagcataa
     2581 agccggcacc cggactgcat tccggtggga aacggcgatt gggaagcaga aaaaaggatg
     2641 gcgggatccc gggttacccg atctttatga gaatggatgc cagggagagg aggagcaggc
     2701 cgttgatcac gacgatccct gcgatgacca tgcccggaac gaggcccggc agaagcatgc
     2761 tgatgccgaa cgcgatcgcc acgacgttca agaccgtcag ggtaccgagg agccgtttgt
     2821 agatgggcgg aaggtgagcc agttcggcgg cgctcccggt gccctgcagc atcgggagga
     2881 accgcttccc gaggagtatc gctcccccgg ctatgttcaa gaacgccagg agcacccgta
     2941 ttgcgtcggt caggatgccc ggaacgatgc aggagaagat ccccagagcg gcaaagacga
     3001 tcccgatgcc gatcaggagc cacgagcgcc tgtactggcc taccggggtc tctcccaggg
     3061 ccagcatctg gatggccatg atgacgagca gcaggccgag ctgaccatcc ggggagaacg
     3121 ggatcaaccc ctggttcacc gggaagagca gcagaccgag gaggaccaga agaacgccga
     3181 ccgggatgat cagggcaatc gagaggggaa gggaggcctc cccgaacagg ccggtgccgc
     3241 cggttgcagt ggaggcgttc ggggtcattc tgcttccccc ggagggtaga gccggccggc
     3301 cttctggatg ctccaggaga ggtagaagaa cccgacgccg tagatgacga ggaagactgc
     3361 agtcacggtg tcggtcgtga tgccgggaag gagcgtgatg atgccgagaa cgatcgagac
     3421 cacgtacaca aggccgcatg cgaccgtcag gtgctggagg gctccgggga ccttcagcca
     3481 tacccgggcc ttatcttcag agaagaacaa ctgcacaagg agcgcgaccc ccccggcaga
     3541 gagaagtatg ccggcaagga tgcggaccgg atcggccagc agcccgggaa tgaagcaggc
     3601 gaccatcccg acgatcgccg tgcatacccc gagcactatg accgcccatg aacgccggag
     3661 gtcgccgaac ggggtcttcc ccatcgtgat gacctgaaac gagacgatca cgagaaacag
     3721 cccgtaggtg ctgtccgggg catacggaag ttcgcccgtc tgaatcctga agagaagcag
     3781 accgaagagg agcatgaaca ctccaaagac gatcaggatg acgacttcga gagagagttc
     3841 ggactctgct ctcacaaccg ggttgttttt ttcccctgac tgactggtaa atcccataat
     3901 tgtatccacc tcgctctatc cgtccatcgc cggatatata tatcacgatt acgtaaatat
     3961 tttgatctgc atccggagtt tatttataag tatgcatccg ctccggctgc gttgcaggtg
     4021 tttcccggcc ctggtcggca ttctctttgc cgcattgctc tgccggcctg gtggcgttgc
     4081 tatgaatacg aagaattgcc gtttgttacg catatggtga catgaaaatg tatcttttca
     4141 aatcgctccc cggggaaagc aagatccaaa acacatatct cttatgggat gacatgagac
     4201 agattggagg atgttatgtt ccgaccgcag gatgatttta cgtacttgat gccggtccac
     4261 ttcggaggcg gcaaattcga cccgggaacc ctgatcacgc agaaggcaac cggtctcgcc
     4321 gtcagttacg agaccgagag agatcttctt gagaactata ttcccgaggg attcgaactc
     4381 ctggcccccg aggtgcaggt tgcgttcaac aagttcaccg agatcaactg gatgcacggc
     4441 ggccagtaca acctgatcaa cgtctcggca ccggtccggt tccacgggaa gaaggacgag
     4501 ctcgacggtg cctacacgct ggttgtctgg gagaacaaga ccgcccccat cctcggggg
//
LOCUS       DAOSII010000142         4523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_16931_length_4523_cov_50.803268, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000142 DAOSII010000000
VERSION     DAOSII010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16931_length_4523_cov_50.803268"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..823)
                     /gene="hisB"
                     /locus_tag="PLV90_08960"
     CDS             complement(239..823)
                     /gene="hisB"
                     /locus_tag="PLV90_08960"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOF97281.1"
                     /translation="MRKGEVRRATRETDISLSLDIDGTGAGAIDTGIPFFDHMLTSFA
                     RHGRMDLTIRAVGDLEVDAHHTIEDIGIVLGAALSEAIGDGRGITRFADAAVPMDEAL
                     ARAALDVGGRGYLVFEGSFSPAGPGGIPGDLIEHFFYSLCTKAGITAHITVAGRNDHH
                     ICEAAFKAFARALRAAVAIDPAMAGDVPSTKGTL"
     gene            complement(810..1529)
                     /gene="hisA"
                     /locus_tag="PLV90_08965"
     CDS             complement(810..1529)
                     /gene="hisA"
                     /locus_tag="PLV90_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOF97282.1"
                     /translation="MEIYPAVDILDGRCVQLVQGRPEAATVYGDPAAWAHRWLEEGAD
                     GIHIINLDGAFGRAQKNVDLIRKFIRETNAFVELGGGIRSVEDAAGWLDVGVDRVILS
                     TLAVREPEVIRALAGEFGSVRVMAGVDARGGEVMIEGWERPAGSYLSWAERFEGLGAG
                     SLLYTNVDVEGLQQGIALAPVEDLLRRVKVPVVVSGGISSAEDARVLREAGAAGAVLG
                     SALYAGRVRLSDVLGAAHAKG"
     gene            complement(1534..2427)
                     /gene="hisG"
                     /locus_tag="PLV90_08970"
     CDS             complement(1534..2427)
                     /gene="hisG"
                     /locus_tag="PLV90_08970"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOF97283.1"
                     /translation="MSRSPFRQQEPGLIRLAIPNKGRISGPVNELIEKSGLHLVDGGE
                     RRLITRTHDPNVEILFARPIDIPEYVASGVADLGITGRDMVMERGSAVEQVLDLQIGR
                     ATLVVAVPEESDVRTAADLDGAKVATEFPAITRAFFAEHGVTVTVVTVGGACEATPHL
                     GIADAIVDLSSSGTTLRTNHLRVIGEVLTSTTVLVANPAALTAKREKIDEIVLALESV
                     IRAKGQCYLMMNVHRSALPDVRQVLPGLSGPTVMDVASDENLVAVHAVVKEERVYQLI
                     NQLKRAGAKDILVMPIERIIR"
     gene            complement(2457..3659)
                     /locus_tag="PLV90_08975"
     CDS             complement(2457..3659)
                     /locus_tag="PLV90_08975"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOF97284.1"
                     /translation="MTRNISVEGLDQIPIERQRIELVERKCLGHPDSLADGIAESISR
                     ALSRSYFEECGSVLHHNTDQGEVVAGESIPKFGGGRITKPIYFLISGRATKTFNGVNI
                     PADAIAVEAARTYIRKILPTLNMERDVIVDCRMGNGSTDLQDVFRSCEGKIPRANDTS
                     FGVGHAPFSEAESIVKGVSAYLDDTLRPKYPVIGQDCKIMCLRDGDAITLTVAMAFVD
                     RYCSGITEYIEQKNVLTEQIAAVAQRFTNRKINVAINTADDLDCGSVFLTVSGTSAEM
                     GDDGSVGRGNRANGLITPNRPMSMEATSGKNPINHIGKIYNLLATRIAQDCIAEIDGI
                     EEMYVRILSQIGHPIDEPHVASAQILTKPGTTVRSIQPDIEGIIDGWMDEITTITEKV
                     IRGELSTF"
     gene            complement(3720..>4523)
                     /locus_tag="PLV90_08980"
     CDS             complement(3720..>4523)
                     /locus_tag="PLV90_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HOF97285.1"
                     /translation="DLMLATACEVAAKIGARAVVSFVDPIPIPPGVPSVPIIRVQELQ
                     LDVLKDLTMHDILEVSERHMLDAAVHLYLRRNLENGLVVGVFPYAVIIYDIEEGKNFI
                     NLKDFSDIVPREVLHAVLSLALEIAVEGREGRSIGTAFIIGDPEEIFRHSHQAILNPY
                     QGHPATLRDIKNKDNWESVKEFAQLDGVFVIDKNGEVRSAGRYLDVTGRGISLPGGLG
                     GRHRATASITYEIPAIGITVSESGGMVRIFRGGNCKIGIRSDIRVRSDG"
BASE COUNT          741 a         1543 c         1396 g          843 t
ORIGIN      
        1 gggggaactc gttccccctc ccctgtctct cacgctccca ggaccccgca catctcaccg
       61 actctacccg tatctccgcc ggcaggctac ccatgcactt ctgccggccc ccccccgccc
      121 gtgaaggcag ggaacagcac ccgtgccccc gtgaaaccgc cggctatcgc gaatcgccac
      181 aacaggtatg acggcgcatc ctcacgcggc gccggaaaag agggcgccgg cccccctctc
      241 agagcgtccc tttggtgctc gggacgtcgc cggccatggc cggatcgatt gccaccgccg
      301 cccggagcgc ccgggcaaac gccttgaacg ccgcctcgca gatatggtgg tcgtttcggc
      361 ccgcgaccgt gatgtgtgcg gttatcccgg ccttcgtgca gaggctgtaa aagaagtgct
      421 cgatgaggtc gcccgggata ccccccggtc ccgccggcga gaaactgccc tcgaagacga
      481 ggtatccccg cccgcccacg tcgagcgccg cccgggcaag cgcctcgtcc atcgggaccg
      541 cggcgtcggc aaaccgggtt atcccccgcc cgtcgccgat tgcctcgcta agggccgccc
      601 cgaggacgat gccgatatcc tcgatggtgt ggtgggcatc cacctcaagg tcccccaccg
      661 cccggatggt gagatccatc cggccgtgcc gggcaaacga cgtgagcatg tggtcgaaga
      721 acggtatgcc ggtatcgatg gccccggccc cggtcccgtc gatatcgagg gagaggctga
      781 tatcggtctc ccgggtcgcc ctgcggacct caccctttcg catgcgccgc ccccaggaca
      841 tccgagagcc ggaccctccc ggcatagaga gccgacccga gcaccgctcc cgccgccccc
      901 gcctcacgga gcaccctggc gtcctcggcg ctcgagatcc cgcccgagac gacgaccggg
      961 accttcaccc ggcggaggag atcctccacc ggagcaagcg cgatcccctg ctggagcccc
     1021 tccacatcca cgttcgtgta gaggagcgat ccggcgccga ggccctcgaa ccgctccgcc
     1081 caggaaagat agctccccgc cggacgctcc cagccctcga tcatcacctc gcctccgcgg
     1141 gcgtcgaccc ccgccatgac ccgaacgctg ccgaactcgc cggcaagcgc ccggatcacc
     1201 tccggttccc ggacggcgag ggtcgagagg atcacccggt cgaccccgac atcgagccag
     1261 cctgcagcgt cctcgacgct cctgatcccc ccgccgagct cgacgaaagc gttcgtctcg
     1321 cggatgaact tccggatcag gtcgacgttc ttctgcgccc ggccaaacgc cccatcgagg
     1381 ttgatgatgt ggatgccgtc cgctccctcc tcaagccacc ggtgcgccca ggcggccggg
     1441 tcgccgtaga ccgtcgccgc ctccggccgg ccctgcacga gctgcacgca ccggccgtcc
     1501 agtatatcga ctgcaggata gatctccatg ctctcagcgt atgatccgtt cgatgggcat
     1561 gactaatatg tcttttgcgc ccgcccgctt caactggttg atcagttgat acacccgctc
     1621 ctctttcacg acggcgtgaa cggcgaccag gttctcgtcg gatgccacgt ccatcaccgt
     1681 cgggccggag aggccgggca gcacctgccg gacgtcggga agagcgctcc ggtggacgtt
     1741 catcatcagg taacactgcc ccttcgcccg gatgacgctc tcgagggcaa ggacgatctc
     1801 gtcgatcttc tcccgctttg ccgtgagggc ggcagggttt gccaccagga ccgtcgtcga
     1861 cgtgagaacc tcgccgatga cccggagatg gttcgtccgg agcgtcgtcc ccgacgaaga
     1921 gaggtcgacg atggcatcgg cgataccgag gtgcggtgtc gcctcgcacg cccccccgac
     1981 ggtcaccacc gtcacggtga cgccgtgctc cgcaaagaac gcacgggtga tcgccgggaa
     2041 ctcggtcgcc accttggcac cgtcaaggtc ggcggccgtc ctgacatcgg actcctccgg
     2101 cacggccacg acgagcgttg ccctgccgat ctgcaggtcg agcacctgct cgaccgccga
     2161 gccccgctcc atgaccatat ccctcccggt gatgccgagg tcggcaacgc cgctcgccac
     2221 gtactccggt atgtcgatcg ggcgggcaaa gaggatctcc acgttcgggt catgcgtccg
     2281 ggtgatgagc ctccgctctc cgccgtcgac caggtggagg ccgctcttct cgatcagctc
     2341 gttgacgggc ccggatatcc tgcctttgtt cgggatggca agacggatga gaccgggctc
     2401 ctgctgcctg aatggtgatc tgctcatgag gtgcgacaac tccggaaccg ggagggttag
     2461 aaggtggaga gttctcccct gatgaccttc tcggttatgg tggtgatctc gtccatccac
     2521 ccgtcgatga tcccctcgat atcgggctgg atcgacctga cggtcgtgcc cggcttcgtg
     2581 agtatctggg cgctcgccac gtggggctcg tcgatcgggt ggccgatctg ggagaggatg
     2641 cggacgtaca tctcctcgat gccgtcgatc tcggcgatac agtcctgggc gatccgggtc
     2701 gcgagaaggt tgtagatctt gccgatgtgg ttgatcgggt tcttcccgct cgtcgcctcc
     2761 atgctcatgg ggcggttcgg ggtgatcagc ccgtttgcgc ggttgccgcg gccgacggac
     2821 ccgtcgtcgc ccatctcggc cgatgtcccg gagaccgtca agaagacgct cccacagtcg
     2881 agatcgtcgg cggtgttgat cgcgacgttg atcttccggt tcgtgaaccg ctgtgcaacc
     2941 gccgctatct gctcggtcag gacgttcttc tgctcgatat actcggtgat gcccgaacag
     3001 tagcggtcca cgaacgccat ggcgaccgtg agggtgatgg catcgccgtc gcgcaggcac
     3061 atgatcttgc agtcctggcc gatgacggga tacttcgggc ggagcgtgtc gtcaagatac
     3121 gcggagacgc ctttaacgat gctctccgcc tcgctgaacg gtgcgtgccc gacgccaaac
     3181 gacgtgtcgt tcgcccgcgg gatctttccc tcgcaggacc ggaagacgtc ctgcaggtcg
     3241 gtcgacccgt tccccatccg gcagtcgacg atgacgtcgc gctccatgtt gagggtgggg
     3301 aggatcttcc ggatgtaggt ccgtgccgcc tcgacggcga tcgcgtcggc aggtatgttc
     3361 acgccgttga aggtcttcgt ggcccgaccc gatatgagga agtagatcgg ttttgttatc
     3421 cggccgccgc cgaacttcgg tatcgactcc cccgcgacga cctcgccctg gtcggtgttg
     3481 tggtgcagga cgctgccgca ctcctcgaaa tacgaccggc tgagcgcccg gctgatcgac
     3541 tccgcgatgc cgtctgcaag gctgtccggg tgcccgaggc acttcctctc caccagttct
     3601 atccgctgtc tctctatcgg gatctggtca agcccttcta cgctgatgtt cctggtcatc
     3661 aaatccaccg tagttctaaa gtatgataag agaaatgatt gcaattcata taaccatttc
     3721 taaccatcac tccggacgcg gatgtcggaa cggatgccga tcttgcagtt gccgcccctg
     3781 aatatgcgca ccatgccccc gctctcggag accgttatgc cgatggccgg tatctcgtag
     3841 gtgatggatg cggttgcccg gtgccgcccc ccgagtcccc ccgggaggga tatgccccgc
     3901 ccggtgacgt cgaggtagcg ccccgccgac cgcacctcgc cgttcttatc gatgacgaag
     3961 acgccgtcga gctgggcgaa ctccttgacg ctctcccaat tatctttgtt cttgatgtcg
     4021 cggagggtgg cgggatgccc ctggtagggg ttgaggatcg cctggtggga gtgccggaag
     4081 atctcttcgg gatcccctat gatgaacgcg gtcccgatgg atctcccctc cctcccctcg
     4141 accgcgatct caagggcgag cgagaggacc gcgtggagca cctcgcgggg gacgatgtcg
     4201 gagaagtcct tgaggttgat gaagttcttc ccctcctcga tgtcgtatat gatgacggcg
     4261 tagggaaaga cgccgacgac gaggccgttc tccaggttcc gccggaggta gaggtggacg
     4321 gcggcgtcga gcatatgccg ctcgctcacc tccagtatgt cgtgcatggt gaggtctttt
     4381 aagacgtcga gctggagttc ctgcacccgt atgatcggga ccgaaggcac gcccgggggg
     4441 atcggtatgg gatcgacgaa cgagacgacc gcccgtgcgc cgatcttcgc cgccacctcg
     4501 caggcggtgg cgagcatcag gtc
//
LOCUS       DAOSII010000143         4433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_17375_length_4433_cov_26.612380, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000143 DAOSII010000000
VERSION     DAOSII010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17375_length_4433_cov_26.612380"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PLV90_08985"
     CDS             <1..180
                     /locus_tag="PLV90_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97286.1"
                     /translation="DTMWELIMDLIGAVIVAGVAAVVLEKKEEDRFTRIFAGMASVAE
                     PADVMGDPADPAESR"
     gene            239..1159
                     /locus_tag="PLV90_08990"
     CDS             239..1159
                     /locus_tag="PLV90_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOF97287.1"
                     /translation="MTLKAPSTRHLALFMLILLPSMTGYMAWEARAVDVTVLAIDGAP
                     EGIVYITDPHVGPSNSDHVREVIREINRLNPSLVLIGGDFTTGEESDFAHQAVWRSLD
                     APAYAVLGNHDYRVGTDGATGIGRILAVRTSANATAAGYDVSALDDGSADTAFADGLA
                     ATLEENGVHVLRNDYARVSAGGEEVVIVGVDDGWAGMADPPDVPATGAFTVYLIHEPS
                     CRADWDADLILAGHTHGGQFLLPVIAQLNGLGVIELAGLFETNGTPTYVSRGICSAGI
                     AGVDLRFNCRPEIVLINPTEEQLRTLGDSA"
     gene            complement(1167..1997)
                     /locus_tag="PLV90_08995"
     CDS             complement(1167..1997)
                     /locus_tag="PLV90_08995"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOF97288.1"
                     /translation="MKHVTKSDGNQQPFDRGKVRRTLQNLGLGAEDADRIADEIEESV
                     PDGMKTAAIFRMIRARARAVRPAVRHRADLRRALTLLRPKPDFEEFVRVLLREHGYQV
                     QTGCVLAGRCGEHEVDAIASKDGVTIFVEVKHHANHHRMTGLDEGRIARAIVEDLQEG
                     FRSGRCTVSIDGALIVCNTKLSEHAKRYAACRGIGHIGWDYPAEQNLRAMIEETQSYP
                     VTIVSGVGPSASTRLAAAGVFTAKQVAYGDATAIARDTGLSLPEVLVIAGRARAILEP
                     "
     gene            2100..3041
                     /locus_tag="PLV90_09000"
     CDS             2100..3041
                     /locus_tag="PLV90_09000"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOF97289.1"
                     /translation="MDALQRPVVLILGAGAVGLSLAGRLLSVATVYAACRPAHAGAIR
                     ERGLVMEGIWGNTTHGGLACVTGPDEVPPGIDFVIITAKGTDTRAICEEYAGQLRDRP
                     VASLQNGIGNEEIIARYAGTVIGGTVTTNFSVVGSGHVRVASESAPMRLGVWSGDERA
                     LDELIGIIRSAGIPVEADGDIRSGKWAKALLNIAVNPICALLAAPVGAAADEDVRETV
                     AGLIRETFAVAGAEGVHLPWASAGDYLAHLFTVQVPDFAAVYPSMYYDLQRGRRTEID
                     LLNGYVVRIGERHGIETPYNRCIAGLVRYAEAHPEPS"
     gene            complement(3046..3225)
                     /locus_tag="PLV90_09005"
     CDS             complement(3046..3225)
                     /locus_tag="PLV90_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97290.1"
                     /translation="MQEYGPGIVGEVRFARQDGGYYVQLYDRGGTPVGKTGLWRSEAK
                     AKEAARKLAAKIGEA"
     gene            complement(3273..3548)
                     /locus_tag="PLV90_09010"
     CDS             complement(3273..3548)
                     /locus_tag="PLV90_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HOF97291.1"
                     /translation="MSLLIAFLALVLVVVVAFVLYRTVKSVTGLIINAVVGVILLWLI
                     NLLGLMHLVGRPDIPINLITVLICAVGGVFGVLVTVVLHLLGISLTL"
     gene            complement(3596..4150)
                     /locus_tag="PLV90_09015"
     CDS             complement(3596..4150)
                     /locus_tag="PLV90_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOF97292.1"
                     /translation="MVWSIIQAILLLSALIPQGHLICDGGWGGPEPFCIDDGAWFSIP
                     EEILSSESTDSSCTGFIEQVDRATFARVDGVPRLFPVGLNATLTRGAGDCTDLALLRV
                     EILRRNGIPARPVHGIMVWDAETFWTDALHMEVFGRGIAVHDWVELDDGQTMGAYEGI
                     PGIRCVKLGNGVCLQPVFEALGYM"
BASE COUNT          814 a         1441 c         1417 g          761 t
ORIGIN      
        1 gacaccatgt gggaactcat catggatctc atcggggcgg tgatcgtggc gggggtcgcc
       61 gctgtcgtcc ttgaaaagaa agaggaagac cggtttacca ggatctttgc cggaatggcc
      121 tcagttgctg aaccggccga cgtcatgggc gacccggcag atccggcgga gagccgctga
      181 tccacccgga cccgggccgc cggaccctat taatatttag aaggtgaaat aaaataaagt
      241 gaccctgaag gccccaagta caagacacct ggcgctgttc atgctcatcc tgctcccgtc
      301 catgaccggt tacatggcct gggaggcacg ggccgtcgac gtgaccgtcc ttgccatcga
      361 cggggcaccc gaaggcatcg tctacatcac cgacccccac gtcgggccgt ccaatagcga
      421 ccacgtccgg gaggtgatcc gggagatcaa ccgcttgaac ccctcgctcg tcctgatcgg
      481 cggcgacttc accaccggtg aggagtcgga cttcgctcac caggcggtat ggcgatcgct
      541 cgacgcaccg gcctacgccg tcctcgggaa ccacgactac cgggtgggga ccgatggtgc
      601 gacaggcatc gggagaatac ttgccgtccg gacctccgct aacgccaccg ccgccggata
      661 cgacgtctcc gccctggacg acggatccgc cgacaccgca tttgccgacg ggctcgccgc
      721 gacgctggaa gagaacgggg tgcatgtcct caggaacgat tacgcacgag tctctgccgg
      781 gggtgaagag gtcgtcatcg tcggcgtcga cgacgggtgg gccgggatgg cggacccgcc
      841 cgacgtgccg gcgaccggtg cattcaccgt ctacctcatc cacgaaccgt cctgcagggc
      901 cgactgggac gccgacctca tcctcgccgg ccacacccac ggcggccagt tcctcctccc
      961 ggtcatcgcg cagctgaacg gcctcggcgt catcgagctt gccggcctct tcgagacgaa
     1021 cgggacgccg acttacgtat cccgcgggat ctgcagcgcg ggcatcgccg gcgtggacct
     1081 ccggttcaac tgccggcccg agatcgtcct gatcaacccg acagaagagc aactccggac
     1141 cctcggcgac tctgcctgag gaccgctcac ggttcgagga tcgcgcgtgc ccgcccggcg
     1201 atcaccagca cttccggaag ggagagaccc gtatcgcggg cgatggctgt ggcgtcgccg
     1261 taggcgacct gctttgccgt gaagaccccg gcagccgcga gccttgtgga ggccgacggc
     1321 cccaccccgc tgacaatggt gaccgggtaa gactgcgtct cctcgatcat cgcccggagg
     1381 ttctgttccg ccgggtagtc ccatccgata tgcccgatcc cccggcaggc cgcgtagcgc
     1441 tttgcgtgct ccgagagttt ggtgttgcag acgatcaggg cgccgtcgat cgagacggta
     1501 caccgcccgg accggaaccc ctcctggagg tcctcgacga tcgcccgggc gatccgtccc
     1561 tcgtcgaggc cggtcatccg gtggtggttt gcgtgatgct tcacctcgac gaatatggtt
     1621 acaccgtctt tactggcgat agcgtccacc tcgtgctctc cgcaccgtcc ggcaagaacg
     1681 cacccggtct ggacctggta accgtgctcc cggaggagaa cccgcacgaa ctcctcgaag
     1741 tcgggttttg ggcggaggag agtgagggct ctccggagat ccgcccggtg gcggacggcg
     1801 gggcggacgg cccgggcccg ggcgcggatc atccggaata tcgcggccgt cttcataccg
     1861 tccggcaccg actcctcgat ctcgtcggcg atccggtcgg catcctccgc cccgagcccg
     1921 aggttctgga gcgtccgccg caccttcccc cggtcgaatg gctgctggtt gccgtcggac
     1981 ttcgtgacgt gcttcattac tgatccatct ctgcacagga gaggaaaagc gttcgccacc
     2041 atatctttca ggcacggccg cacccttatc ttctctcgca tccaacagag gctgagagaa
     2101 tggatgcatt acaacgcccg gtcgtcctga tactcggcgc aggagccgtc ggcctctcgc
     2161 ttgccgggag gctcctttcg gtcgcaacgg tctacgcggc atgccggccg gcgcatgccg
     2221 gtgcgattcg ggagcggggc ctcgtgatgg agggtatctg ggggaacacc acccatggag
     2281 ggctcgcctg tgtcacgggg ccggatgaag tcccgcccgg gatcgatttc gtcatcatca
     2341 ccgcgaaggg caccgacacc cgggcaatct gcgaagagta tgccgggcag ctccgggatc
     2401 gacccgtggc aagcctccag aacggtatcg ggaacgagga gatcatcgca cgatacgccg
     2461 gcaccgtcat cggcgggacc gtgacgacga acttctcggt cgtgggctca gggcacgtcc
     2521 gggtcgcgag cgagagcgcc ccgatgcgcc tcggggtctg gtccggcgat gagagggccc
     2581 tcgatgagct gatcggcatc atccggtccg ccggcatacc ggtcgaggcg gacggcgaca
     2641 tccgttcggg aaaatgggcg aaggccctcc tcaacatcgc ggtgaacccg atctgcgccc
     2701 tccttgcggc cccggtcggc gccgccgccg atgaagacgt cagggagacc gttgccgggc
     2761 tcatccgcga gacattcgcc gtcgccggcg ccgagggggt gcacctcccc tgggcgagcg
     2821 ccggcgacta tctcgcccac ctctttaccg tgcaggtgcc cgacttcgcc gcggtctacc
     2881 cctccatgta ctacgacctc cagagggggc gccggacgga gatcgatctc ctcaacgggt
     2941 atgtcgtgcg catcggagag cgccatggca tcgagacgcc gtacaaccgg tgcatcgccg
     3001 gtcttgtccg ctacgcggag gcgcacccgg aaccgtcgtg agagattagg cctcgcctat
     3061 ctttgcggcg agtttccggg ccgcctcttt tgcctttgcc tcgctcctcc agagccccgt
     3121 cttgccgacg ggggttcctc cccggtcata gagctgcacg tagtagccgc cgtcctggcg
     3181 ggcaaaccgg acctcgccga cgatgccggg accgtactcc tgcatacccg gagggtatgg
     3241 aagacggaga tatgagggtt tcgcccgccc actcacagtg tcagcgatat gccgaggagg
     3301 tgcagcacca ctgttaccag tacgccaaag accccgccga ccgcgcagat gaggacggtg
     3361 atcaggttga tggggatgtc gggccggccg acgaggtgca tcaggccgag gaggttgata
     3421 agccagagca ggataacgcc cacgacggcg ttgatgatga gcccggtgac gcttttgacc
     3481 gtcctgtaga ggacgaacgc gacgacgacc accaggacca gagcaaggaa tgcgatgagc
     3541 aagctcatac tacctgttcg gcacccgggg cattgaactt tccccggggc accgttcaca
     3601 tatacccgag agcctcaaag actggttgga ggcagacccc gttcccgagt ttgacgcacc
     3661 ggataccggg aatcccttcg taagcgccca tcgtttggcc atcatcaagc tcgacccagt
     3721 cgtggacggc gatgcctcgc ccgaagacct ccatgtggag cgcatcggtc cagaacgtct
     3781 ccgcatccca gaccatgatc ccgtggaccg gccgggcggg gatcccgttc cggcggagga
     3841 tctccaccct gaggagtgca aggtcggtgc agtcgcccgc tccacgggtt agggtcgcgt
     3901 tgaggcccac cgggaagagg cgggggacac cgtcgacgcg tgcaaacgtc gcccgatcca
     3961 cctgctcgat gaacccggtg caggaagaat ccgtactctc ggaagagagg atctcttccg
     4021 gtatagagaa ccaggcccca tcgtcgatac agaacggttc ggggccaccc catccgccgt
     4081 cgcagatcag gtgtccctgc gggatgaggg cggagaggag gagaatagcc tgaataatgc
     4141 tccaaaccac gataattgct caatactttt taatgaataa attagtataa tagaattgct
     4201 atttgatgtc gagtatcgcg ggttcgcaat ataactatcg cgaccgtaga tacgccggga
     4261 tcggctctgg cgcgcaacaa ggaatatatg cccccgggcg ggagggagaa gaggtcgact
     4321 ccccggaggg cgacaggttc accgggatat ggggtgcccg gcgatatccc gtgcggcaag
     4381 ggccgcggtg gagaatgccg cctgcaggtt gtagcccccg gtgtcgccgt cga
//
LOCUS       DAOSII010000144         4417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_17454_length_4417_cov_55.511463, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000144 DAOSII010000000
VERSION     DAOSII010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17454_length_4417_cov_55.511463"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PLV90_09020"
     CDS             <1..507
                     /locus_tag="PLV90_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HOF97293.1"
                     /translation="VIARDTEVDRLHWLVARQENLIVIDAALSRRMDVTVSQAAYYFQ
                     VSRIVERIADHATQVAHNANALIDREAGGTAMVGAMDEASTLALEIFSESMGALYTGD
                     VRKANATLQMVRDLEMKTRDIDTRALDLEAVAAIPIGQISASIRRIGEYSGDICECVI
                     NYTVGQAL"
     gene            575..1825
                     /locus_tag="PLV90_09025"
     CDS             575..1825
                     /locus_tag="PLV90_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOF97294.1"
                     /translation="MGTFIPIVVLVVVFLLIAVRKVGNTSLCIWQVVLSGAAAVLVAG
                     AITPADALLSINPDVMLFLFFMFVIGEALAESGYLYHLSYRLFSRAESVRHLVVLILA
                     GGGILSALLMNDTLAVVGTPLMLYFARRHGISAKLLLLSLAFAITTGSVVSPIGNPQN
                     LLIALSGGIGNPFVTFPLYLAVPTAISLLLAYIFLCRAFPGEFHRDPLVHRKEEIRDP
                     ALAALARLSLVLLVLLIAAKVAAAVLVPDIEFRLTWIAVVAAMPVLIGSSRRFELVRR
                     IDWATLVFFAGLFVLMASVWQSGFFQPLIEESSLDLAAVPVIMAMSVVVSQFVSNVPF
                     VALSLPVLAHLGTSTTGMMALAAGSTIAGNMLILGAASNVIVIQGAERGGETLTFGEF
                     ARVGVPLTVAQAAVYVLYLALFPA"
     gene            complement(1801..2685)
                     /locus_tag="PLV90_09030"
     CDS             complement(1801..2685)
                     /locus_tag="PLV90_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp1/GlmU family protein"
                     /protein_id="HOF97295.1"
                     /translation="MIRIGEGWEDLAAALDGATPERVYVVGPTDSGKTTLCRYLAEQA
                     FAHAQTAYVDCDTGQSRIGPPTTEGMALSSGPTYIRFVGSTSPGGHFIQTLTAAKRLV
                     EKAAGARVTVIDSPGLVAGRVGAEFQFHMIDLLCPTRIVALQRGRELERLLANFARSP
                     KTAVHRLPVSPAAVARTATGRRRYREERFRDYFSGSRVQEVALRGLGLQGRLPDMANP
                     RGIEGRLVSFNDPENFALALGIVEEISPGERTLRALAPPFDHAAVASVRFGSLALDLD
                     AGPGLMESLHPYAGKSAR"
     gene            2782..3303
                     /locus_tag="PLV90_09035"
     CDS             2782..3303
                     /locus_tag="PLV90_09035"
                     /inference="COORDINATES: protein motif:HMM:NF017027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97296.1"
                     /translation="MHGTAATTEPVPAAIGRTFPALMATSLTGRVVTLPDDAGGDVAL
                     IVLVFLESASPMAESWSGPFAGAFTRNPRVTTYLVSVVGDSLIGRSLADQIARGLRGG
                     VPPAKHDTVLTVPGDIEGCRRAYGVDDPSLAYIYLLDGRGIIRWMEKGTATPEGLDTL
                     VEAARGLLEPLAR"
     gene            complement(3328..3804)
                     /locus_tag="PLV90_09040"
     CDS             complement(3328..3804)
                     /locus_tag="PLV90_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOF97297.1"
                     /translation="MEIVKIPSMDKAEYDKLIEEGFVSRIAFQGSKYPYIAPFLYVFD
                     GKFLYFLATRYGRKNDLFRQHPYVSVEIEKYSSDLSSYTFVTMQGYLVQVEDAIEKKI
                     IREKFVSMIRAHNLSPNILAALGHKPEEPIEAIASEERSNIWKLTGVTDIVALKNL"
BASE COUNT          678 a         1471 c         1442 g          826 t
ORIGIN      
        1 gtcatcgccc gggatacgga ggtggaccgg ctgcactggc tcgtcgcgcg gcaggagaac
       61 ctcatcgtga tcgatgccgc gctctcgcgc cgtatggacg tcacggtgag ccaggcggcg
      121 tactacttcc aggtcagccg gatagtcgaa cggatagccg atcacgcaac acaggtggcg
      181 cacaacgcga acgccctgat cgaccgcgaa gccgggggaa cggcgatggt cggtgccatg
      241 gatgaggcaa gcacgcttgc gcttgagatc ttctcggaga gtatgggagc gctctacaca
      301 ggggacgtca ggaaggcgaa cgcaaccctc cagatggtgc gcgaccttga gatgaagacc
      361 cgggatatcg atacccgggc cctcgacctc gaggcggttg cggcgatacc catagggcag
      421 atatcagcga gcatccggag gatcggggaa tattccgggg atatctgcga gtgcgtcatc
      481 aactacaccg tcgggcaggc cctctgagag ccggggcagc accctttttt gggccggcgg
      541 ccgacgttcg atcagcatat ttgccggtgg tagcgtgggc accttcatcc cgatcgtcgt
      601 ccttgtcgtc gtctttctcc tgatagccgt ccggaaggtc gggaacacga gcctctgcat
      661 ctggcaggtg gtgctctctg gagcggctgc ggtccttgtc gcgggtgcga tcacgcctgc
      721 ggatgcactt ctgtccatca accccgacgt gatgctcttc ctcttcttca tgttcgtgat
      781 cggggaggcg ctcgcagaga gcggctacct ctaccacctc tcgtaccgcc tcttctcccg
      841 ggcggagtcg gtccggcacc tggtcgtcct catcctcgcc ggcggcggca tcctctcggc
      901 gctcctgatg aacgatacgc tcgcggtcgt cggcacgccg ctcatgctct acttcgcccg
      961 gcggcacggc atatccgcaa aactcctgct gctctcgctc gcgttcgcca taacgacggg
     1021 gagcgtcgta agccccatcg ggaacccgca gaacctgctc atcgcgctct cgggcggcat
     1081 cggaaacccg ttcgtcacgt tccccctcta ccttgcggtc ccgacggcga tatcgctcct
     1141 gctcgcctac atcttccttt gccgggcatt tccgggcgag tttcacagag acccgctggt
     1201 ccaccggaag gaagagatcc gcgaccccgc cctcgcggcg ctcgcccgcc tctcgctcgt
     1261 cctgctcgtc ctcctgatcg ccgccaaggt cgccgcggcc gtgctcgtcc cggatatcga
     1321 gttccggctg acctggatcg cggtcgttgc ggccatgccc gtcctcatcg ggagttcgcg
     1381 gcgctttgag ctcgtccgcc gtatcgactg ggcgacactg gtcttcttcg ccggcctctt
     1441 cgtcctcatg gcgagcgtct ggcagtcggg gttcttccag ccgctcatcg aggagagttc
     1501 gctcgacctt gcagcggtcc cggtcatcat ggcgatgagc gtcgtcgtct ctcagttcgt
     1561 ctcgaacgtc cccttcgtcg ccctctccct cccggtcctt gcgcacctcg ggacttccac
     1621 gacgggcatg atggcgcttg cggccgggag caccatcgcc gggaacatgc tgatcctcgg
     1681 cgcggcaagc aacgtcatcg tcatccaggg cgcggagagg ggcggcgaga cgctgacgtt
     1741 cggcgagttc gcccgggtcg gcgtcccgct caccgtcgct caggcggcgg tctacgtgct
     1801 ctatctcgcg ctcttcccgg cataggggtg gagcgactcc atcagccccg gccccgcatc
     1861 gaggtcgaga gcgagcgagc cgaacctgac cgacgcgacg gcggcgtggt cgaagggcgg
     1921 tgcgagggcg cgaagggtcc gctctccggg ggagatctcc tcgacgatgc cgagggcgag
     1981 ggcgaagttc tccgggtcgt tgaaggagac gagcctcccc tcgatcccgc gggggtttgc
     2041 catgtccggc agccgcccct ggagcccgag cccccggagc gcgacctcct gcacccggct
     2101 gccggaaaaa tagtcccgaa atcgctcttc acggtagcgc cgcctcccgg ttgcggtccg
     2161 ggcgacggcc gccggcgata ccgggagccg gtggacggcg gtctttgggg accgggcgaa
     2221 gtttgcgagc agccgttcca gttcccgccc gcgctggagg gcgacgatcc gggtggggca
     2281 gagcaggtcg atcatatgga actggaactc ggcgccgacc ctgccggcga cgaggccggg
     2341 cgagtcgatg acggtgacgc gagccccggc ggccttctcg acgaggcgtt ttgccgccgt
     2401 cagcgtctgg atgaagtggc cgccggggga ggtcgacccg acgaaccgga tgtaggtcgg
     2461 gccggaggag agggccatcc cctcggtcgt cggcgggccg atccgggact gcccggtgtc
     2521 gcagtcgacg tatgcggtct gcgcgtgggc gaaagcctgc tcggcgaggt agcggcagag
     2581 cgtcgtcttc ccgctgtccg tcggtccgac gacgtatacg cgctcagggg tcgctccgtc
     2641 gagcgccgcg gcaaggtcct cccatccttc cccgatccgg atcatatcgc gatagtattg
     2701 ccgtccaccg gtaagtgcct gacgaaccgc tcccgtgagc gggagggagg gtttatctgg
     2761 atcacccgcc atccccgtcc catgcacgga acggcagcca ccaccgaacc ggtgcccgcg
     2821 gccatcggca ggacgtttcc cgccctcatg gccacgtcgc tcaccggccg ggtcgtcacc
     2881 cttcccgacg acgccggggg cgacgtcgcg ctcatcgtcc ttgtcttcct cgagtcggcc
     2941 tcgccgatgg cggagtcctg gagcggcccc ttcgccgggg cgttcaccag gaacccccgg
     3001 gtgacgacct acctcgtctc ggtcgtcggc gacagcctca tcggcaggtc tcttgccgac
     3061 cagatcgccc gggggttgcg gggcggcgtc ccgccggcaa agcacgatac ggtgctgacc
     3121 gtgcccgggg atatcgaggg atgccggcgg gcgtacgggg tcgacgatcc gtcgctcgcc
     3181 tacatctacc tcctggacgg gcgcggcatc atccgctgga tggagaaggg gaccgccacg
     3241 ccggaggggc tcgatactct ggtggaggcg gcccgggggt tgctcgaacc gctcgccagg
     3301 tgacggggaa ccgcccggca ggaagggtca gaggttcttc aacgcgacga tatcggtcac
     3361 gccggtgagc ttccatatgt tcgagcgctc ttcggaggcg atggcctcga tcggctcttc
     3421 gggcttgtgg ccgagcgcgg caagtatgtt cggcgagagg ttgtgcgccc gtatcatgct
     3481 cacgaacttc tcccggatga tcttcttctc gatggcatcc tcgacctgca cgagatagcc
     3541 ctgcatcgtc acgaaggtgt aactggagag atcgcttgag tacttctcga tctcgaccga
     3601 gacgtagggg tgctgccgga agaggtcgtt cttcctgccg taccgtgtcg cgaggaagta
     3661 gaggaacttt ccgtcgaaga catacaggaa cggggcgatg tacgggtact tgctcccctg
     3721 gaacgcgata cggctgacga acccctcctc gatgagtttg tcgtactctg ctttatccat
     3781 gctcgggatc ttcacgatct ccatcaggca tcctcgtatt ttcggtgacg tgtgagagta
     3841 catataggtt tccccggcgg gcgtctcttc atccgcttcc gggtggatgc actccgggtg
     3901 ccatcccggc ggcaaaagac ccatccgccg gcctccggcc gaaattgcgg cgtatcggct
     3961 cccgatagcc ggtgcgccgg acggatgagg gcgctgcgct gttgagccgc tgcactccgt
     4021 gccccctttc gcgccaacgg ggctcatctt cgcgacgata cccgccccgt cgcagggccg
     4081 ggtgcgacga tccggggacc gatctggtga gatacccctc tcccccggaa ggccgcccgc
     4141 ccgccgcctc accagggccg ggcaccggcc ggcaccggaa acggtttggt ctcggggcct
     4201 cttttttcaa acaggcaata taaaaatatt tatatttaac aaccgaaaga aaaatgcgca
     4261 tgagtaagca ccatgcgacc gctctgcttc tcctcgggat tgccgtgtgc gcggggttct
     4321 tcctctccgt gccggccgcc gccggcccgg gcaaggggtt cgaggcgctg gagtccgggg
     4381 acctcttccg gtccccgttc ggcaccacgc cgccgga
//
LOCUS       DAOSII010000145         4343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_17855_length_4343_cov_49.429571, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000145 DAOSII010000000
VERSION     DAOSII010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17855_length_4343_cov_49.429571"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PLV90_09045"
     CDS             <1..236
                     /locus_tag="PLV90_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5714 domain-containing protein"
                     /protein_id="HOF97298.1"
                     /translation="SLANQMTARSLMTIARHGGPRCCKRDSWLAIRAAVTFLSERFGI
                     TLPVPETVRCEFSDINRECLREACPFHAGYATG"
     gene            complement(221..409)
                     /locus_tag="PLV90_09050"
     CDS             complement(221..409)
                     /locus_tag="PLV90_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97299.1"
                     /translation="MASFEREILVLFVSFLIGSVAGWWSRVYWESSLAVVAATLAGTV
                     AGYCIITVALRAAGHPVA"
     gene            506..1192
                     /locus_tag="PLV90_09055"
     CDS             506..1192
                     /locus_tag="PLV90_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOF97300.1"
                     /translation="MFVACHLLLGLIIGLVIARHLGDRRLIGLAALGAILPDLIDKPV
                     GHILLAESLNSGRLFTHGLLFLALLLMAGIILERRRGSFALLAVAAGVLSHQILDAMW
                     AAPVAWYFPFLGPYQPHEFTDYFGNAILAEVSSLSEWIFLAAAAAIILALSRDTRPGF
                     LAIARTLARVAIPLLMLLTLASLYAWAAGIPESILMAGGSGEDYLLLAAVGAAGIAGM
                     VRYRDLLDLG"
     assembly_gap    1253..1352
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1353..1533)
                     /locus_tag="PLV90_09060"
     CDS             complement(<1353..1533)
                     /locus_tag="PLV90_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOF97301.1"
                     /translation="MSSSSDAGFTGLEAAIVLIALVIVAAVFGFVILQAGFTSAQQGQ
                     SVIHDGMEQAGSSCMV"
     gene            complement(1517..2074)
                     /locus_tag="PLV90_09065"
     CDS             complement(1517..2074)
                     /locus_tag="PLV90_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOF97302.1"
                     /translation="MSREGKNEQAFTGLEAAIIFIAFVVVASVFSYVVLGMGMTTSQK
                     SQEVVHAALDEARSAFRPGDAVIVKLDTAGGLSFIEFDLETAAGPTAIDMKSMTCTLA
                     TSKALLTYPPGDPAVKLTWRYCKDTDDILEAGEIVTVRLTPGSKGIKRGDTFTIGVVA
                     AGGATASLTRTIPAGARGNTYVELF"
     gene            2317..2955
                     /locus_tag="PLV90_09070"
     CDS             2317..2955
                     /locus_tag="PLV90_09070"
                     /inference="COORDINATES: protein motif:HMM:NF019380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1616 domain-containing protein"
                     /protein_id="HOF97303.1"
                     /translation="MPPEASPGPLSGPPRTCTVLPGLTSSCPDASLSIAAQGESDDLS
                     GGSAPRFERVLSLVLIAAVLAALGTTIFIIVSPGEGEKFTEFYILGPTGKAADYPTEF
                     MSRTPQTVIIGIGNHESRDITYTVETFAVESGFDSAKNRPTVVSATLLDRFSVTVPDN
                     RTVEQPYTFRIEDPDVDRLEFLLFAEAPPEEIPLDSLIGAGYRNLHLQLRVH"
     gene            3145..3639
                     /locus_tag="PLV90_09075"
     CDS             3145..3639
                     /locus_tag="PLV90_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97304.1"
                     /translation="MKRGILVASLLILSLVAAPAGAFSADDLRITLDTDGSADVTFNY
                     TLSWLERIAVFFRIAEPEKELKNALETATGSPVTVTSAMSDSATFSIRNFAKVRSTDE
                     GSVYSTPALDFTGAQEVLDSYWFAPLVKADFSPDLTLVRFPDGHEETFANQYAIPALS
                     HTIP"
     gene            complement(3860..>4343)
                     /locus_tag="PLV90_09080"
     CDS             complement(3860..>4343)
                     /locus_tag="PLV90_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HOF97305.1"
                     /translation="EIRGKVENAMLKLGDKWRKKDFESLGEGKDRLKKIMNDTSRCIK
                     CYACIENCPICYCVECSTRKPYLVTPGQVPPPFMFHLIRYAHISDSCINCGQCEENCA
                     MDIPNALFMHALQVDLEEMFGHVPGVDMELPVLALVEERTERKRLSDTGSDQIFDIFK
                     "
BASE COUNT          767 a         1387 c         1262 g          827 t
ORIGIN      
        1 ggtcgcttgc aaaccagatg accgcacgca gcctgatgac gatcgcccgc cacgggggtc
       61 cccggtgctg caaacgcgac tcatggcttg cgatccgggc ggcggtcacg ttcctctcgg
      121 agcggttcgg cataaccctc cccgtgccgg aaacggtgcg gtgcgagttt agcgatatca
      181 accgcgagtg cctgcgagag gcgtgcccct tccatgccgg ctacgcgacg ggatgacccg
      241 cggcccggag ggcgacggtg atgatgcagt aaccggccac ggtcccggcg agggtcgcgg
      301 caacgacggc gaggctgctc tcccagtata cgcggctcca ccagcccgct acgctgccga
      361 tgaggaacga aacaaagagt acgaggatct ctctctcgaa tgaggccacg accgctctcc
      421 cttcaaccgt cggttctctc cggacataat cgtttgcgca cggatagcag ggttatatcc
      481 cggccgggca ccgatctcat gagggatgtt cgtcgcctgc cacctcctgc tcggcctcat
      541 catcggcctc gtgattgccc ggcacctcgg cgaccggcga ctcatcgggc ttgccgctct
      601 cggggccatc ctccccgacc tgatcgacaa accggtgggc cacatcctgc ttgcagagtc
      661 gctcaactcc ggcaggctgt tcacacacgg gttgctcttc cttgcgctcc tccttatggc
      721 cggcatcatc cttgaacggc gacgaggatc gtttgcgctc cttgccgtcg cggcgggcgt
      781 gctctcccac cagatcctcg atgcgatgtg ggcggccccc gtcgcctggt acttcccgtt
      841 cctcggcccg taccaaccgc atgagttcac cgattacttc ggcaacgcca tacttgcaga
      901 ggtctcgtcg ctctcggagt ggatcttcct tgccgccgcg gccgcgatca ttcttgctct
      961 ctcccgggac acccggccgg gctttctcgc gatcgcccgc acgctcgccc gcgtggcaat
     1021 cccgctcctt atgctcctca ctctcgcttc cctctacgcc tgggcggccg gcataccgga
     1081 gagcatcctg atggccgggg gcagcgggga agactacctg ctgcttgcgg cagtgggagc
     1141 cgccggaatt gcggggatgg tccggtaccg tgacctcctc gatctcgggt aggccgtcac
     1201 tccagccggt tcacccgctc gatgctgccg ggaatcttcc gctcgacccg gannnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcaccatg cagctcgatc ccgcctgctc
     1381 cataccgtcg tggatgaccg actggccctg ctgtgcggag gtgaaaccgg cctgcaggat
     1441 gacgaacccg aagaccgcgg caacgatgac gagcgcgatc aggacgatcg ccgcctcaag
     1501 cccggtgaac ccggcatcag aagagctcga cataggtatt ccccctggcg ccggcaggaa
     1561 ttgttctcgt cagcgaagcg gtcgcacctc ccgcggcgac cactccaatg gtgaacgtat
     1621 ctccgcgttt gatgccctta ctgcccgggg tcaacctcac cgtgacgatc tcccccgcct
     1681 ccagtatatc gtcggtgtcc ttgcaatatc gccaggtgag ctttaccgca gggtcaccgg
     1741 gaggatacgt gaggagcgcc tttgatgtgg cgagggtgca ggtcatgctc ttcatatcga
     1801 tggcggtcgg tccggccgcg gtctcgaggt cgaactcgat gaacgagaga ccgcctgccg
     1861 tatccagttt tacgatcact gcatccccgg ggcgaaaagc agatctggcc tcgtcgaggg
     1921 cggcgtgcac cacctcctgg ctcttctgcg acgtcgtcat gcccatcccg agaacgacgt
     1981 aggagaagac cgatgcaacg acgacgaagg cgatgaagat gatagccgcc tcaaggccgg
     2041 tgaaggcctg ctcattcttc ccctcccggg acataatgat cgggtttggg cgtaccgtga
     2101 ttaaaacatg ctggtatcac ccggagatag caggattttg cggccggcag gagggcagcc
     2161 gtgacgagat atgtgcccgg gcgcactccg ggagagaggg caaaatccgt tcggcaccct
     2221 ctgttcgtga ggctcccggt tctcccggat cgatatacgg ggcgagggtc tgccgccgga
     2281 aacctattta tacctggcct atcatggggt aacagaatgc ctcctgaagc ctctccgggt
     2341 cctctctccg gccccccgcg cacctgcacc gtcctgccgg gtctaacgag cagttgcccc
     2401 gatgcatccc tctcgatcgc ggcacagggc gaatctgacg acctctccgg cggttccgcc
     2461 ccccggttcg agcgggtcct gagcctcgtc ctcatcgccg ccgtcctcgc ggctctcggg
     2521 acgacgatct tcatcatcgt ctcccccgga gagggggaga agttcaccga gttctacatc
     2581 ctcgggccga cggggaaggc agccgattac ccgaccgagt tcatgtcccg gacgccgcag
     2641 acggtcatca tcgggatcgg gaaccacgag tcacgggaca tcacctacac ggtggagacg
     2701 tttgccgtgg agagcgggtt tgatagtgcg aaaaaccggc cgacggtcgt ctcggcgacg
     2761 ctccttgacc ggttctccgt cacggtgccg gataaccgga ccgtcgaaca gccgtacacc
     2821 ttccggatag aggatccgga tgtggaccgg cttgagttcc tcctctttgc ggaggcgccg
     2881 cctgaggaga taccgctgga tagcctcatc ggggcgggct accgtaacct gcacctccag
     2941 ctccgggtgc actgacgggc gcgaagggtt atcgggggca ccaggggccc cggatggcag
     3001 gaggcccctt cgccggattt ttacagtttt ctcttttttc tgcgagcgat gccggaaaat
     3061 ttccgatatc cggccacgat acgcaggggc aaccctccaa atatttatac cgcatgacgg
     3121 cgtatggttc atcgaggtat aaggatgaaa cgcggaattc tggtcgcctc cctcctgatc
     3181 ctgtcgcttg tggcagcccc ggccggggcg ttcagcgcgg acgacctccg gataactctg
     3241 gatacggacg ggagcgcaga tgtcaccttc aactacacac tctcgtggct cgagaggatt
     3301 gccgtcttct tccgtatcgc cgagcccgag aaggagctca agaacgcgct cgagacggca
     3361 accgggtctc cggtaacggt gacctcggcc atgagcgact ccgccacgtt ctcgatccgg
     3421 aactttgcaa aggtcaggag caccgatgag ggcagcgtct actcgacgcc ggcactcgac
     3481 ttcaccgggg cgcaggaggt gctcgacagt tactggtttg caccgctggt caaggccgac
     3541 ttctccccgg acctgacgct ggtcaggttc cctgacggcc acgaagagac cttcgcgaac
     3601 cagtacgcaa tcccggctct ctcccacaca atcccctgaa ccggccccta tcctttcttt
     3661 tttcactcac acaagataga gaggtacggc ctctcacccc gggcgctgga gccgggtcta
     3721 acacccggga ggtatgacga tgcccggcaa tgaatcgtat agaatcactt gcgttgccgc
     3781 gaaggcgagc aggacgagca gatcgcacgc gatgaaccgc ccaccacgga tgccaaaaaa
     3841 atagagggga aaactcccct tacttgaaga tgtcgaagat ctggtcgctg ccggtgtcag
     3901 agagacgctt cctctccgtc ctctcctcga cgagtgcgag gaccgggagt tccatatcca
     3961 cacccgggac gtggccgaac atctcctcaa gatcgacctg cagggcgtgc atgaagagcg
     4021 cgttcggtat atccatcgcg cagttctctt cgcactggcc gcagttgatg catgagtcgg
     4081 atatgtgggc gtaccggatc aggtggaaca tgaacggcgg cggaacctga ccgggcgtga
     4141 ccaggtaggg cttcctcgtg ctgcactcga cgcagtagca gatcgggcag ttctcgatgc
     4201 aggcgtagca cttgatgcac cgggacgtgt cgttcatgat cttcttcagg cggtccttcc
     4261 cctcaccgag gctctcgaag tccttcttcc gccacttgtc gccgagcttc agcatcgcgt
     4321 tctcgacctt gccgcggatc tcg
//
LOCUS       DAOSII010000146         4242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_18399_length_4242_cov_41.721280, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000146 DAOSII010000000
VERSION     DAOSII010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18399_length_4242_cov_41.721280"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..1021
                     /locus_tag="PLV90_09085"
     CDS             104..1021
                     /locus_tag="PLV90_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOF97306.1"
                     /translation="MEKTPFSEWLDRFSNYLRMRNYSPRTIRKYEQTIERFARYAWFR
                     EHSGPGPFDETACGDAPLDADVNVSAALVTDFFSALSEERDYKPKTLHRMISTLSSFY
                     RYLYAQGAVVANPMPGVERPRVKNQELKYLKHSQVVRLLNTIPTGRDRLIVRLIYATG
                     VRVSELCAINVEDVDFEEQTIRVKGKGDKFRTVFVDEETLGEIERFVGNSIEGPLFLG
                     QQGNHISPRTVQHLFRQYAPPGITPHKIRHSYASELYRRSKNLRVVQENLGHSSIKTT
                     EIYLHTDIEERRSVYRQYFPLSNGKKIDE"
     assembly_gap    1120..1219
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1474..2409
                     /locus_tag="PLV90_09090"
     CDS             1474..2409
                     /locus_tag="PLV90_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOF97307.1"
                     /translation="MRYIVTGGAGFIGSNLAEHLARDHDVVIIDNLSSGRRENIEHLI
                     GHPRVTFIEGSVTDLALLTGAFAGADGIFHQGAIASVPRSVEKPLETNAVNVGGTVNV
                     LWAAKECGVPAVVAASTSAIYGDDPVFPKHETMAPTPLSPYAVSKLAGEYYGKVFSDL
                     YGIRTVSLRYFNVFGPRQDPNSEYAAVIPKFITRLLAGKPPIIYGDGEQTRDFIFVAD
                     VVRANVQAMEGNATGVFNIAGGRRISLNELAARLSGIAGMHARPLYGPVRPGDVRDSL
                     ADISRARDAFGFSPRSTVDEGLRETVAWFRDRGSL"
     gene            2579..2950
                     /locus_tag="PLV90_09095"
     CDS             2579..2950
                     /locus_tag="PLV90_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOF97308.1"
                     /translation="MTATDLHDPAVLNNRGVALERQGRYEEALAAFSAALLCDDDDVY
                     AWNNRAVILTRLGRPQEALLACKEALAVDRSCIFAWVTYGTVLGRIGRYVEAESAFAV
                     AEDLDPQARALYPGNSTMTRA"
     gene            3143..>4242
                     /locus_tag="PLV90_09100"
     CDS             3143..>4242
                     /locus_tag="PLV90_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOF97309.1"
                     /translation="MKTLILAGGSGTRLFPLSREHYPKQFIPLFDNESLFQKTIKRAL
                     LFSSPQDIAIVTNKEHRFLVRDQLAAIGCNSKVIEEPVGRNTLPAIYYGVREVARGEP
                     ETVAVLPSDHLITATGSFLAAFRRAEHLTKDYLVVFGVRPTSPHTGYGYIRPGDSLGD
                     GSLVDAFVEKPDLATAGRYVADGYLWNSGMFCFDAGLFLAECETCAPDVVRAFERPAD
                     EAYHAVPSLSIDYGIMEKTRRAAVVPLECDWNDVGNFDALYAALPKNGDKNAVRGEHI
                     GIDSSENLIIADRLVATVGVRDLAIVETKDAILVAARNEAQRVGEIAKTLREKGDSRA
                     LFHTQVHRPWGSYTNLEEGRSYKIKRVTVPPKR"
BASE COUNT          784 a         1366 c         1260 g          732 t
ORIGIN      
        1 cccggaccgg ggccgggcag gggtttttga ggtgagatct ctattgcggg gaatagtgat
       61 ttaccgcaat cttgatattc ttcgatatct aacccattat ccgatggaaa aaacgccctt
      121 ctcggaatgg ctggatcgtt tcagcaacta cctccggatg cggaactact cgccccggac
      181 catccggaaa tacgagcaga ccatcgagcg ttttgcccgg tatgcctggt tccgggagca
      241 ttcgggtccg ggcccgttcg atgagacggc gtgcggggat gcccccctgg atgcggacgt
      301 caacgtctct gcggccctcg tcaccgattt cttctccgcc ctctcggaag aacgtgacta
      361 taagccgaag actctccacc ggatgatatc gacgctctcc tcgttctacc gctatctcta
      421 cgcacagggg gccgttgtcg caaaccccat gcccggtgtg gagcggcccc gggtcaagaa
      481 ccaggaactg aagtacctca agcacagcca ggtggtccgc ctgctcaaca ccatcccgac
      541 cgggcgggac cggctgatcg tccgcctcat atacgccacc ggcgtccgcg tttcagaact
      601 ctgcgcgata aacgtcgagg atgtcgactt tgaggagcag acgatccggg tgaaggggaa
      661 gggcgacaag ttccggacgg tcttcgtcga cgaggagacg ctcggcgaga tcgagcggtt
      721 cgtcgggaac tcaatcgagg gcccgctctt tttgggccag cagggcaacc acatctcccc
      781 gcgaaccgtc cagcacctct tccggcagta tgctccgccg gggattacgc cgcacaagat
      841 ccgccattcc tacgcaagcg aactctaccg gcgctcaaag aacctccgcg tcgtgcagga
      901 gaacctgggc cactcttcca tcaaaacgac cgagatctac ctgcataccg acatcgaaga
      961 gcggagaagc gtctaccggc agtatttccc gctctcgaac gggaagaaga tagacgagta
     1021 gccggcggtc gataaaccgc gtgatgctat atgggttgga ggtggcgggg gatccatccg
     1081 gcccggttct tcgggagagc tcaccggagg ctctcctccn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnna atgggtgcag tcgcccggca cgagaggctc tctccccccg
     1261 ttgccggcgg aagtttgagc gccagacgga ttacggctgc aggtacccgg ctgccgaact
     1321 tcgggtcctt cgcggcttcg cgcccgggta tacgagcgat aaaggtagcc tctttgcatg
     1381 gagtgcgaga tgcgattgga aggatcaggg cccatcgtct ttcgccctcc cgaccggcaa
     1441 cgctcaaatt gcaggagcac ctaccgtatt cgtatgcgct acatcgtcac aggcggagcg
     1501 ggattcatcg gttcgaacct tgcggagcac ctggcacgcg accacgacgt cgtgatcatc
     1561 gataacctca gctccgggcg gcgggagaat atcgagcacc tcatcggtca tccccgggtg
     1621 acgttcatag aggggagcgt caccgacctt gccctgctca ccggtgcctt tgccggcgcg
     1681 gacggcatat tccatcaggg ggccatcgcc tcggtccccc gctccgtgga aaaacccctg
     1741 gagacgaacg cggtgaatgt gggcggaacc gtgaacgtcc tctgggccgc aaaggagtgc
     1801 ggcgtcccgg cggttgtggc ggcctctaca tcggcgatct acggcgacga cccggtcttt
     1861 cccaaacacg agacgatggc gccgacgccg ctctccccgt acgcggtctc aaagctcgcg
     1921 ggcgagtact acgggaaggt cttctccgac ctctacggca tcaggacggt atccttacgc
     1981 tacttcaacg tctttggccc ccggcaggac ccgaactcgg agtacgcggc ggtgatcccg
     2041 aagttcatca cccgcctgct cgccggaaaa ccgccgatca tctacggcga cggggaacag
     2101 acccgcgact tcatctttgt cgccgacgtg gtgcgggcga acgtccaggc gatggagggc
     2161 aacgccaccg gcgtcttcaa catagccggg ggccgccgga tcagcctcaa cgagctcgct
     2221 gcccgcctct cggggatcgc cggcatgcac gccaggccgc tctacggacc ggtgcggccg
     2281 ggggacgtgc gtgactcgct tgccgacata tcgcgggcca gggatgcttt tggcttttct
     2341 ccccggagca cggtcgacga agggctccgc gagaccgtgg cgtggttccg ggaccggggt
     2401 tctctataac ccttcttctc cggcggccgg cccccccgtt cccggcatgg tgggtgcagg
     2461 tatctatata tcttcgctcc accgtatgcg tgcgaggtgc caatgtatgc cccggctgat
     2521 tacaccggag accccccgca agggtgacga cagatatgct tcacgggcgt acagcgatat
     2581 gacagcaacg gatctgcacg atcccgcggt cctgaacaac aggggcgtgg cgctcgagcg
     2641 gcagggccgt tacgaggagg ccctggccgc gttcagtgcg gccctcctct gcgacgacga
     2701 cgacgtctac gcctggaaca accgggcagt gatcctcacc cgcctcggcc gcccccagga
     2761 agccctgctt gcatgcaaag aggcgctcgc ggtcgaccgg tcatgcatct tcgcctgggt
     2821 cacctacggc acggtcctcg gcaggattgg aagatacgtg gaggccgagt cggcctttgc
     2881 ggtcgcagag gacctcgacc cgcaggcgag agcgctctac cccgggaact cgacgatgac
     2941 ccgggcgtga gggatcgcgt cgccctgccc gcgatacacc gcccggtgcg atacggaggg
     3001 gtgccggcag atccgatcga tcgctctccc gaactgctcg cacgacatcg ccgggcctcc
     3061 accgaaaaaa ccctacgctt ttatcctcca taccgcaata gagtaatcac aatcgcgtgc
     3121 cccggcaccg gagtcgatcc tgatgaagac gctcattctt gcaggcggaa gcggcacccg
     3181 cctcttcccg ctctcaaggg aacactaccc aaaacagttc atcccgctct tcgacaacga
     3241 atcgctcttc cagaagacga taaagagggc gctcctcttc tcctccccgc aggacatcgc
     3301 catcgtcacc aataaggagc accggttcct ggtccgggac cagcttgccg cgatcgggtg
     3361 caactcaaag gtcatcgagg aaccggtggg caggaacacc ctgccggcga tctactacgg
     3421 cgtccgggaa gtcgcccggg gcgaaccgga gacggtcgcg gtgctgccct cggaccacct
     3481 gatcaccgca accggatcgt ttcttgcggc cttccgccgg gcggagcacc tcacgaaaga
     3541 ctacctggtg gtctttggag tgcggccgac gtcgccccac accggctacg gctacatccg
     3601 cccgggcgac tccctcgggg acggttcgct cgtggatgcc ttcgtggaga aacccgacct
     3661 tgcgaccgcc gggaggtacg tcgccgacgg ctacctctgg aactccggca tgttctgttt
     3721 cgatgccggc ctcttccttg ccgagtgcga gacgtgcgcg ccggacgtgg tccgggcgtt
     3781 cgagcggccc gccgatgagg cataccatgc cgtcccctcc ctctccatcg actacggtat
     3841 catggagaag acccggcggg cggctgtcgt gccgcttgag tgcgactgga acgacgtggg
     3901 gaacttcgac gcgctttacg ccgcactgcc aaagaacgga gacaaaaacg ccgtcagggg
     3961 cgagcatatc ggtatcgact cctccgagaa cctgatcata gccgaccgcc tggtcgccac
     4021 cgtcggcgtc cgcgaccttg ccatcgtcga gacgaaggat gcgatcctcg tcgccgcccg
     4081 aaacgaggcg cagcgggtgg gcgagatagc aaaaaccctc cgcgagaagg gcgactcccg
     4141 tgcgctcttc cacacgcagg tccaccggcc ctggggctcg tacacgaacc tcgaggaggg
     4201 gcggtcctac aagatcaagc gggtcaccgt ccccccgaag cg
//
LOCUS       DAOSII010000147         4195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_18685_length_4195_cov_46.514976, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000147 DAOSII010000000
VERSION     DAOSII010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18685_length_4195_cov_46.514976"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..701
                     /locus_tag="PLV90_09105"
     CDS             9..701
                     /locus_tag="PLV90_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOF97310.1"
                     /translation="MAALEQVAPSELAEDYDAGRIGLIVEGREDIGTVCCALDATQRV
                     ADAAVRAGADMLIVHHTPLWNPVTAVRGPTAHLLRTLLAAGMNLYVMHTNFDRAEGGV
                     NDVLAARLGLTATERMTAGVVGDCSLDPEEIARRLPGGGIRVYGEVGTIRRLAVVGGS
                     GFDPALLREAADLGAEAFLSAELKHSVARASPLPCFEATHYALEAPAMEALASRMGWH
                     YIPDPPHVVVIP"
     gene            698..1015
                     /locus_tag="PLV90_09110"
     CDS             698..1015
                     /locus_tag="PLV90_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HOF97311.1"
                     /translation="MSGIWQKMERGGALTPEFRACIEQAYGERGRKALMAVDSGQVKR
                     YLDFFVVVGHSDEYIVEDDFCTCSDFLFRGRECWHILAVRIAERTGVYESYDLWYQDV
                     WKR"
     gene            1078..3816
                     /gene="alaS"
                     /locus_tag="PLV90_09115"
     CDS             1078..3816
                     /gene="alaS"
                     /locus_tag="PLV90_09115"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOF97312.1"
                     /translation="MLEEEYTLDYFRSQGFERKVCKSCGAAFWTRDPEQELCGDAPCV
                     TYNFIGNPVFKPHTVDEMREAFLSFFERHGHTRLERYPVAARWRDDIYLTIASIADFQ
                     PFVTSGVVPPPANPLTISQPCIRLNDLDSVGRSGRHLTLFEMMAHHAFNTPEERIYWK
                     DETVALCDEFIASIGGDLSRISYKEHPWYGGGNAGASVEVLIGGLEVATLVFMNLGRQ
                     KTNQLPVNVDGELYYPMRLQIVDTGYGLERFVWASKGSPTIYDAVFPEMVSRLMRSAH
                     LENLLDSPEFMKIMGLSARFAGVMDISGTNLYNLRKKVADAIGVPVERLERIVVPIEK
                     IYSIADHTRCLAYMLGDCIVPSNVREGYLARLVLRRTLRMMNDLSMDEDLADLVEAQM
                     QAVGDFSQDVDVVREIVASEEARYAGTLERGTRIVQKIARNYRAKSSRVPLAEVITLY
                     DSHGIPPEMVKEVAAAEGAVVEIPDNFYSLIADIHSEAQKEAEGADPLAPYRERAATL
                     PPTKKLYYELPCEVEFEAMVLDYFDGMAVLDQTLFYPEGGGQPSDTGTLVTSESMVRV
                     EEAVKIGEVILHRVTGGPLKRGDRVKGMVDEERRLSLMRHHTATHVLLHAAKKVLGAH
                     VHQAGAQKGSETSRLDVRHYRHITPDELRRIEIEANRMVMADLPVYICIEERTKAEQK
                     YGFDLYQGGVPPGREIRTVQVGAEVQACAGTHVRATGEIGPIRVIGVEHIQDGVERLV
                     FAAGLAAVYAIQRMADILGASADVVSVQPENLPATVTRFFSEWKEQKKEIERLRKSAV
                     DLRMQHLAGEVVDGVRVVVGQVDAAPKELVALATSVADEGGVALFVSVDGNVKVVATS
                     GVPAVNAADIVREVCSIIGGKGGGKPTLAQGAGSDVARLGQALEHGRSRILEALHG"
     gene            3809..>4195
                     /locus_tag="PLV90_09120"
     CDS             3809..>4195
                     /locus_tag="PLV90_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOF97313.1"
                     /translation="MVDGVVVIQPGDERAQRIARAMASQTANAIIQAFGSGPLTSSEV
                     ARSMAIPITTATYHIDNLLNAGLIEVMETRWSEKGREVKVYGLANQVVIIAPPESDLR
                     SVLKKYATLFGIVVLASVGLWGILPAV"
BASE COUNT          742 a         1302 c         1461 g          690 t
ORIGIN      
        1 cgaggttcat cgccgcactg gagcaggttg ctccgtccga actggccgaa gactacgatg
       61 ccgggcggat cggcctcatc gtcgagggga gagaagatat cgggaccgtc tgctgtgcgc
      121 tcgatgccac gcagagagtg gcggatgccg ccgtccgggc cggcgcggat atgctgatcg
      181 ttcaccacac gcccctctgg aacccggtca cggcggtcag gggtccgacg gcgcacctgc
      241 tccggacgct tcttgccgcc ggcatgaacc tgtacgtgat gcacacgaac ttcgaccgtg
      301 ccgagggcgg cgtcaacgac gtcctcgcgg cccggctcgg tctcacggca acggagcgga
      361 tgaccgccgg cgtcgtcggg gactgctccc ttgacccgga agagatcgcc cgccgcctcc
      421 ccggcggagg gatacgggtc tacggggagg tgggcacgat ccggcgcctc gcggtcgtgg
      481 gggggagcgg gtttgacccc gcactcctcc gggaggccgc cgacctcggg gcggaggcct
      541 tcctctcggc ggaactgaag cacagcgtgg cgcgggcatc gcccctcccc tgctttgaag
      601 ccacccacta tgcgctcgag gccccggcca tggaggcgct cgcgtcccgc atggggtggc
      661 attatatccc tgatccgcca cacgtagtgg taatcccatg agcggcatct ggcagaagat
      721 ggagcggggc ggggcgctga cgccggaatt ccgcgcctgc atcgaacagg cctacggcga
      781 gcgggggagg aaggcgctca tggccgtcga tagcgggcag gtgaagcgct accttgactt
      841 cttcgtcgtg gtgggccaca gcgacgagta catcgtcgag gacgacttct gcacctgcag
      901 cgatttcctc ttccggggaa gagagtgctg gcacatactt gcggtgcgca tagcagaacg
      961 gacgggggtc tatgaatcct acgatctctg gtaccaggac gtatggaagc ggtgaaagat
     1021 acgctccaaa caactatatc cgttccagtc ctacctcttt gtactacgtg attttcaatg
     1081 ctcgaagaag agtatacgct cgattatttc cgatctcaag gatttgagcg gaaggtctgc
     1141 aagagctgcg gggcggcctt ctggacccgg gaccctgaac aggagctctg cggcgacgcc
     1201 ccgtgtgtga cgtataactt catcggcaat ccggtcttca aacctcatac cgttgatgag
     1261 atgcgggagg cgttcctctc gttcttcgag cggcacgggc atacccgcct cgagcgctac
     1321 ccggtcgccg caaggtggcg agacgacatc tacctcacga tcgcgtccat cgccgacttc
     1381 cagccgttcg tcacgagcgg tgtcgtcccg ccgccggcaa acccgctcac catctcccag
     1441 ccctgcatcc gcttaaacga cctcgactcc gtggggaggt cgggccgcca cctgacgctc
     1501 tttgagatga tggcgcacca tgccttcaac acccccgagg agcggatcta ctggaaggac
     1561 gagacggtcg ccctctgcga cgagttcatc gcgtccatcg ggggcgacct ctcccggatc
     1621 agctacaagg agcacccctg gtacggcggc gggaacgccg gggcctccgt cgaggtgctc
     1681 atcgggggcc ttgaggtcgc aaccctggtc ttcatgaacc tcgggcggca gaagaccaat
     1741 cagctgcccg tcaacgtgga cggggaactc tactacccga tgcggttgca gatcgtcgat
     1801 accggctacg gcctcgagcg gttcgtctgg gcatcgaagg gttcgccgac gatctacgat
     1861 gcggtcttcc cggagatggt gagccggctg atgcggtcgg cccacctcga gaacctcctc
     1921 gacagcccgg agttcatgaa gatcatgggg ctcagcgccc ggttcgccgg ggtgatggac
     1981 atatccggga cgaacctcta caacctccga aagaaggtcg ccgatgcgat cggcgtgccc
     2041 gtcgagcggc tggagcggat cgtcgtcccg atcgagaaga tctactcgat cgccgaccac
     2101 acccgctgcc tcgcctacat gctcggcgac tgcatcgtcc cctcgaacgt ccgcgagggg
     2161 tacctcgccc ggctggtgct gcgccggacg ctccggatga tgaacgatct ctcgatggac
     2221 gaggatctcg ccgacctcgt ggaggcgcag atgcaggccg tcggggactt cagccaggac
     2281 gtcgatgtgg tcagggagat cgtggcaagc gaggaggcgc ggtatgcggg cacgctcgag
     2341 cgcgggaccc ggatcgtcca gaagatcgcc cggaactaca gggcgaagag ctcccgcgtg
     2401 cccctcgcgg aggtcatcac cctgtacgac tcgcacggca taccgcccga gatggtgaag
     2461 gaggtcgcgg ccgccgaggg cgccgtcgtg gagatccccg acaacttcta ctcgctgata
     2521 gccgacatac actcggaggc gcagaaggag gcggaagggg cggatccgct cgccccctac
     2581 cgcgagcggg cggccacgct ccccccgacg aagaaactct actacgagct cccctgcgag
     2641 gtcgagttcg aggcgatggt gctcgactac ttcgacggca tggcggtgct ggaccagacc
     2701 ctcttctacc cggagggggg cggccagccg tccgataccg gcaccctggt gacgtcggag
     2761 agcatggtgc gggtggagga ggccgtgaag atcggggagg tgatcctcca ccgggtgacg
     2821 ggaggccccc tgaagcgcgg cgaccgggtg aaggggatgg tggacgagga gcggcggttg
     2881 tcgttgatgc gccaccatac ggcgacccac gtcctcctgc acgcggcaaa gaaggtgctc
     2941 ggcgcccacg tgcaccaggc gggcgcccag aaggggagcg agacctcccg cctggacgtc
     3001 cggcattacc ggcacatcac gcccgacgag ctccggcgga tcgagatcga ggcgaaccgg
     3061 atggtgatgg cggacctgcc ggtctacatc tgcatcgagg agcggacgaa ggccgagcag
     3121 aagtacgggt tcgacctcta ccagggcggg gtgccgccgg gccgggagat caggacggtg
     3181 caggtcggcg ccgaggtgca ggcgtgtgcg gggacgcacg tccgggcaac gggtgagatc
     3241 gggccgatcc gggtcatcgg cgtcgagcat atccaggacg gcgtcgaacg gctggtgttt
     3301 gcggccgggc ttgccgccgt ctacgcgatc cagcggatgg cggatatcct tggtgcgtcc
     3361 gccgacgtgg tgagcgtcca gcccgagaac ctgccggcga cggtaacgcg cttcttctcg
     3421 gagtggaagg agcagaagaa ggagatcgag cgtctccgga agagcgcggt ggacctccgc
     3481 atgcagcacc ttgcgggcga ggtggtggat ggggtccggg tcgtcgtcgg tcaggtggat
     3541 gcggctccta aggagttagt tgcgcttgcg acgagcgtcg ccgacgaagg cggggtggcg
     3601 ctctttgtat cggtcgacgg aaacgtgaag gtggtggcga cctcaggcgt gccggcggtg
     3661 aacgccgccg atatcgtgcg ggaggtctgc agcattatcg gcgggaaggg cgggggcaag
     3721 ccgacccttg cgcagggcgc aggatcggac gtcgcccggc tcggccaggc gctcgagcac
     3781 gggcgcagcc gtattctcga agcactccat ggttgacggt gtcgtagtta tccagccggg
     3841 cgatgagcgg gctcaacgga tcgcccgggc gatggcgagc cagacggcga acgcgatcat
     3901 tcaggccttc ggcagcgggc cgctgacgtc gtcggaggtc gcccggtcga tggcgatccc
     3961 gatcaccacc gccacctacc atatcgataa cctcctgaac gccggtctca tcgaggtgat
     4021 ggagacccgc tggagcgaga aggggcgcga ggtgaaggtc tacggcctgg caaaccaggt
     4081 cgtcatcata gcgccgccgg agagcgacct ccggtcggtg ttgaagaagt acgcgacgct
     4141 ctttggcatc gtcgtcctcg cgagcgtcgg gctctggggt atcctcccgg cggtg
//
LOCUS       DAOSII010000148         4177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_18786_length_4177_cov_46.884279, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000148 DAOSII010000000
VERSION     DAOSII010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18786_length_4177_cov_46.884279"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..770)
                     /locus_tag="PLV90_09125"
     CDS             complement(87..770)
                     /locus_tag="PLV90_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HOF97314.1"
                     /translation="MPADTLPLIYDALLAAFGHRHWWPAETAFETMIGAILTQNVSWT
                     NAARAIRNLEDAGMLDPHLLAGAGADEIARRIVPSRFYNQKAERVREFSRVYVAEFAA
                     DPAVMAAVETGTLRERLLAVRGLGKETVDSILLYACGKPVFVVDAYTRRIFSRYGLLP
                     PDASYDRMQRLFMENLPPDAALFNDYHAQVVFLGNTVCKKSPLCDRCPIRAVNGQVRC
                     AGVPPHFFL"
     gene            complement(770..2368)
                     /locus_tag="PLV90_09130"
     CDS             complement(770..2368)
                     /locus_tag="PLV90_09130"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOF97315.1"
                     /translation="MKIALLQVNTVAGDIAGNAGRIAAGAGEAARHRPDLVVTPELAL
                     PGCPPRDLLLERGFVERSLAVLDDLAADLGGAPPVLVGLAAPNLSGKGRSLFNAAALL
                     RGGAVEATFNKARLSPGIFDEDRYFEPGVPQAFRLAGQTIGVSIGEELLSGIPIGADV
                     VVNLSASPFAAGHHRLREEAFARTAKDLEVAIARANLVGGNDDLVFEGRSFAVSAGGT
                     LLARGAAFEEDVVIVDTARPAPRPIAPAGPAPEPDIWGALVLGTRDYARKCGFSSAHL
                     GLSGGIDSSLVAAIAVEALGPENVLGVLLPSPYTSRESIEDAREVAANLGIRAECIPI
                     APLMEAFDGALEDMFAGLPRDTTEENLQARIRATVLMALSNKFGSLLLSTGNKSEAAV
                     GYSTLYGDMAGGLAVIADLSKGMVYRLARWLNTRQPVIPGRVLDKPPSAELRPGQTDQ
                     ETLPPYDILDAILSLSIERFESPREIVAAGYPPATVYRVLGMVRRAEFKRRQAPPGLR
                     VTGRAFCTDRRMPIAGKAWWREEG"
     gene            2592..3905
                     /locus_tag="PLV90_09135"
     CDS             2592..3905
                     /locus_tag="PLV90_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF97316.1"
                     /translation="MSAGKRGGNRLLLLAASVAATFAVNAAGLLAGITIVLPHLFYLP
                     IVLAAYWFPRRGIVASLAIAVGYLAMTLPVAGGDIGLIVSALSRAAVFIAIGAAISFL
                     SLRLGEQEERYRGIFGNSEAGMFIVALAETVPRIEEANYVGATLLGATAGALIGEPMS
                     RFFADPGTWDEIRSGNAVYGYETALRRTDGTAVQVFLSAGRLPGERVVLTLIDITARK
                     NAENALREANAKLNMMGRLTRNDLMAAVSTLLSRIAGGIREFDDPAFLRYLDGLDEDA
                     RFVQRRAEITRDYQDLGLRPPGWQPLQKTIQEVRSCLLLPGVSVRPWVERLEVYADPM
                     LDRVFSNLIENAARHGKSVSQVVITYQIRDDGLLVYVEDDGVGIPEPKKEEIFEYSAG
                     GGGGLGLFFVREILSITGMTIAETGTPGEGARFEIHVPPDGYRIV"
     gene            3915..>4177
                     /locus_tag="PLV90_09140"
     CDS             3915..>4177
                     /locus_tag="PLV90_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GCN5 family acetyltransferase"
                     /protein_id="HOF97317.1"
                     /translation="MFRKVFIPTDLSGASMSPAERLGEIPGAREAVFFHARTSALPDT
                     GLLHRMSDALQRQGFVVSTTIAEDDGTPIVERILRAAADAGADL"
BASE COUNT          721 a         1398 c         1409 g          649 t
ORIGIN      
        1 aagagcggga agtcgatcac gatcgcctcc accgagggca acgtctcggt gccggggcac
       61 gaagatatca agatcggcct gaacatctac aaaaagaagt gagggggaac cccggcgcac
      121 cggacctgac cgttgaccgc ccggatgggg cagcggtcgc agagcggcga ctttttgcag
      181 acggtgtttc caagaaagac tacctgggcg tggtagtcgt tgaagagcgc cgcgtcaggg
      241 ggaaggttct ccataaagag tcgctgcatc cggtcgtagg aggcgtcggg aggaaggagg
      301 ccgtagcgcg agaatatccg ccgcgtgtag gcgtcgacga cgaagaccgg ctttccgcag
      361 gcatacagca ggatcgagtc caccgtctcc tttccgagcc cccggacggc aagcagccgc
      421 tcgcggaggg tgccggtctc cacggcggcc atcaccgccg gatcggccgc aaactccgcg
      481 acgtagaccc gggagaactc ccggacccgc tccgccttct ggttgtagaa ccgcgacggg
      541 acgatccggc gagcaatctc atccgccccg gccccggcaa gcaggtgcgg gtcgagcatg
      601 ccggcgtcct ccaggttccg gatagcccgg gccgcgttcg tccaggagac gttctgggtg
      661 aggatcgcgc cgatcatcgt ctcgaacgcc gtctctgccg gccaccagtg ccggtggccg
      721 aacgccgcaa ggagggcatc gtagatcagg gggagggtat ccgccggcat caaccctctt
      781 cccgccacca cgcttttccg gcgatcggca tacgccggtc ggtgcaaaac gccctcccgg
      841 tcaccctgag ccccggcggg gcctgccggc gcttgaactc cgcccgccgg accatgccga
      901 ggacccggta gacggtcgcc ggggggtacc cggcggcgac gatctcccgc ggggactcga
      961 agcgctcgat ggacagggag agtatggcat cgaggatatc gtaggggggg agggtctcct
     1021 ggtcggtctg gccgggccgg agctccgcgg aggggggttt atcgagcacc cgcccgggta
     1081 tgacgggctg ccgcgtattc agccaccggg cgagccggta gaccatcccc ttcgagagat
     1141 cggcgatcac ggcaagaccg cccgccatat ccccataaag ggtggagtag ccgaccgccg
     1201 cctccgactt gttcccggtc gagaggagca gggagccgaa cttgtttgat agagccataa
     1261 ggaccgttgc ccgtatacgg gcctgcaggt tctcctcggt ggtgtcgcgg ggaagcccgg
     1321 cgaacatatc ctcgagggcg ccgtcaaatg cctccatcaa gggggcgatc ggtatgcatt
     1381 ccgcccggat gccgaggttt gccgcaacct cccgggcatc ctcgatgctc tcccgcgacg
     1441 tgtaggggga cgggaggagg acgccgagga cgttctccgg cccgagcgct tcgacggcga
     1501 tagcggccac gagcgacgag tcgatccccc ccgagaggcc gaggtgggcc gacgaaaacc
     1561 cgcacttgcg ggcgtagtcg cgggtgccga gcaccagcgc cccccatatg tcgggctccg
     1621 gcgccggacc ggcgggagcg atcggccgcg gggcggggcg ggcggtatcg acgatcacga
     1681 cgtcctcctc gaatgccgca ccgcgggcga gaagggttcc gccggcgctg acggcgaaac
     1741 tccggccctc aaagaccagg tcgtcgttgc cgccgacgag gtttgcccgg gcgatcgcga
     1801 cttcgaggtc ctttgcagtc cgggcgaacg cctcctcccg caggcggtga tgcccggcgg
     1861 caaacggcga tgcggagagg tttacgacca catccgcgcc gatagggatg ccagagagga
     1921 gctcctcgcc gatagagacc ccgatcgtct gcccggcaag gcggaatgcc tgcgggaccc
     1981 ccggctcaaa gtaacggtcc tcatcgaaga tgcccgggga gagccgggcc ttgttgaacg
     2041 tcgcctccac cgcccctccc cggaggagag cggcggcgtt gaagagcgat cgacccttcc
     2101 cggagaggtt cggtgcggca agaccgacga ggaccggcgg cgcgcctccg agatcggcgg
     2161 cgagatcgtc gaggacggcg agacttcgct ccacaaaccc tctctcgagg aggaggtccc
     2221 ggggcgggca gccgggcagg gcgagctccg gtgtcacgac gaggtccggc cggtggcggg
     2281 cagcctcccc ggcacccgcc gctatccgcc cggcgttgcc ggcgatatcg ccggcgaccg
     2341 tgttcacctg gaggagtgcg atcttcatga atcttgatca gatatgacga aacggagagc
     2401 atatgccttt gcatggctct gccggagctg caggcaccga tcacggctct acggcgatcg
     2461 aatccctttt aagtgcctaa gatcaaagcg ccatagagaa taaccatgca gagagcacat
     2521 cgcaggacag cacccgcccg gggttttgga aagccgtttt cgtgccgtat tctccacggg
     2581 aggcaggcag catgagcgcc gggaagcgcg gaggtaaccg gctcctcctg ctcgcggcat
     2641 cggtcgccgc caccttcgcc gtaaacgccg ccgggctcct tgccggcatc accatcgtcc
     2701 tcccgcacct cttctacctg ccgatcgtgc ttgccgcgta ctggttcccc cgccgcggga
     2761 tcgtcgcctc gcttgccatc gccgtcggct acctggccat gaccctcccc gttgccgggg
     2821 gagatatcgg gctgatcgtc tcggcactct cgcgggcggc ggtcttcatc gccatcggcg
     2881 cggccatatc gttcctctcc ctgaggctcg gtgagcagga agaacgctac cggggcatct
     2941 tcggcaactc ggaggcgggg atgttcatcg ttgccctggc tgagaccgtg ccgcgcatcg
     3001 aggaggccaa ctacgtgggc gcgaccctcc tcggcgccac ggccggcgcc ctgatcgggg
     3061 agcccatgag ccggttcttc gccgaccccg gcacctggga cgaaatccgg tcgggtaacg
     3121 cggtctacgg ctacgagacg gccctccgcc gcaccgacgg gaccgccgtc caggtcttcc
     3181 tctcggccgg ccgtctcccg ggcgagcggg ttgtcctcac gctcatcgat atcaccgccc
     3241 gaaagaacgc cgagaacgcc ctccgggagg ccaacgccaa actgaacatg atggggcgcc
     3301 tcacccgtaa cgacctgatg gcggcggtat cgacgcttct ttcgcggatc gccggcggca
     3361 tccgggagtt cgacgacccg gccttcctcc ggtacctcga cggcctcgac gaggatgcac
     3421 ggttcgtgca gcgccgagcc gagatcaccc gtgactacca ggacctcggc ctccggccgc
     3481 cgggctggca acccctccag aagacgatcc aggaggtgag atcctgcctg ctgctccccg
     3541 gcgtatcggt ccgcccctgg gtggagcggc tcgaggtcta tgccgacccg atgctcgacc
     3601 gggtcttttc gaacctgatc gagaacgccg cccgccacgg gaagagcgtc tcgcaggtcg
     3661 tcatcaccta ccagatccgg gacgacgggc tcttagtcta cgtcgaggac gacggcgtcg
     3721 gtataccgga gccaaagaag gaggagatct tcgagtacag cgccggcggc gggggcggtc
     3781 tcgggctctt cttcgtgcgg gagatcctct cgatcaccgg catgacgatc gcggagaccg
     3841 ggacacccgg cgagggagcg cggtttgaga tccacgtccc gccggacggc taccggatcg
     3901 tctgaggtgg aaccatgttc agaaaagtct tcattccaac cgacctctcc ggggcctcga
     3961 tgagcccggc ggagcggctc ggggagatcc ccggcgcccg tgaggccgtc ttcttccatg
     4021 cccggacgtc ggccctgccc gacaccggac tgctccaccg aatgtcggac gcgctgcagc
     4081 gccagggatt tgtggtatcg acgacgatcg cagaagacga cgggacgccc atcgtggaga
     4141 ggatcctccg ggccgccgcc gatgcgggtg ccgacct
//
LOCUS       DAOSII010000149         4175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_18798_length_4175_cov_53.629369, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000149 DAOSII010000000
VERSION     DAOSII010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18798_length_4175_cov_53.629369"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            359..1384
                     /locus_tag="PLV90_09145"
     CDS             359..1384
                     /locus_tag="PLV90_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97318.1"
                     /translation="MHCPVCGHDCVTGARELLLTLPERFAPCPDCTGLVYDKRSPPPD
                     IDAAEPCPSCGKRFIDEVFADIYRAMAAEGDLSGTEPLAAAGTPLVHPGFAMRRPPYL
                     PPRSLVLLSRSIGEQAAARLVATVPEVRGVIRAGTGTPGIRDTDTEPETNTLLAGCDV
                     RADVFSTRAGPVVIYKQQSALHVEFPRDRDEKILSLEREIGRHRPRTFVDACSGAGTL
                     ALAAARAGIPRVIANDAWYAAAYWTACNLQVNREHLGIEGVTMHRSYDDLRRREVARE
                     PLRVATAAGAREVEVYQGDLRLLSTVLPPGIDLTAIDLFEKADAEKTDRIVRAWRARV
                     GGAIFIP"
     gene            1397..1707
                     /gene="ffs"
                     /locus_tag="PLV90_09150"
     ncRNA           1397..1707
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLV90_09150"
                     /product="signal recognition particle sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01857"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal-type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01857"
     gene            1719..2672
                     /locus_tag="PLV90_09155"
     CDS             1719..2672
                     /locus_tag="PLV90_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOF97319.1"
                     /translation="MAKVTILGATGNVGMFAARTVSEIPHVSEMLLVGRPGREDFLAG
                     CCRDLSDSFAARGTHVRLSYSTRLTDVEGSDIVICTAGVPRRPGQDRNDLAFENAKIV
                     AGSAETIGRSSPDAILFMVTNPVDVMTAVALKYSGLQSRQVFGLGTHLDSMRLKSLIA
                     HYFRVHVSEVHTRIIGEHGESMVPLWSATTIGGIRISNLPTFSGLPAQEMIGAVRTSG
                     ETIIREKGSTVYGPGEAIATLVRTILGDENRILTVSSYITSEIHGIGDVCIGVPARLN
                     RSGAFPVPIRLEENEVAGFEESVKKIRKVTADVMERLGEAH"
     gene            complement(2700..2954)
                     /locus_tag="PLV90_09160"
     CDS             complement(2700..2954)
                     /locus_tag="PLV90_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 1-beta"
                     /protein_id="HOF97320.1"
                     /translation="MGNVAIILKIMPESPDVDREALKAAVRGAVPVNDIREEPIGFGL
                     VALKIAVVVPDKAGAPDEVESALQNLEGVGSAEIIESTLV"
     gene            complement(2958..3122)
                     /locus_tag="PLV90_09165"
     CDS             complement(2958..3122)
                     /locus_tag="PLV90_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HOF97321.1"
                     /translation="MTARDRCTSCNATLAEEGSTGFPCPVCAHEINRCYRCREQSIAY
                     TCQKCGFRGP"
     gene            complement(3168..3770)
                     /locus_tag="PLV90_09170"
     CDS             complement(3168..3770)
                     /locus_tag="PLV90_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridylate kinase"
                     /protein_id="HOF97322.1"
                     /translation="MTTPAPPLVVKVGGSLFDRVAPLLGILREVGRPVLIVPGGGKFA
                     DLVRRLAVSDTSAHWMAIAGMEQFGWYIASHGIPAVASPVLPAEATVLLPYCALRKAD
                     PLPHSWNVTSDTIAAWVADELSTDLILLKSVDGIHHRRRLLSRVEDPSLTADEVDPAF
                     IGFVFEHDIVVRVVNGRHDDRVRGVLRDEAVTGTLIDPRF"
     gene            3810..>4175
                     /locus_tag="PLV90_09175"
     CDS             3810..>4175
                     /locus_tag="PLV90_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HOF97323.1"
                     /translation="MRRIAAIILLLCLLAGTAGGILIVEFCPDPYRPGDPDEYLVLEG
                     EGSLDRFVVSDGKGGFRFPPGTWINGRLVIARNSLAFEQTHGYLPDFEIEKRTAAVPD
                     VTSSGNLLMANQADALYLYH"
BASE COUNT          780 a         1338 c         1342 g          715 t
ORIGIN      
        1 tccgctactt cccggagaac cgcctccgtg acgcctgggg atggctccgg gaaggatcgg
       61 tccagatccc tctggcggcg caatgaggat gatgagtcac ggtgcaggaa gggatggcag
      121 acagcacggg agactgcaaa gagggtgcgc aacactgcag gatgcaggag ggacccgcct
      181 cctccaggga tgagcagcgg atcagcgccg gacaagaacg gatcgtcgag atctcaaaga
      241 ggtggccccg gaatacctat gcagcatgga gaacgaccgc aatgcttgcg gtgctgatgg
      301 aaccggccga agagggggca tgaaagacct aaataccccg gcccccaacc catccggtat
      361 gcactgtccg gtctgcgggc acgactgcgt cacgggcgcc cgcgagcttc tcctgaccct
      421 ccccgaacgg ttcgccccct gccccgactg caccgggctc gtctacgaca aacggagccc
      481 gccgcccgat atcgatgccg cggaaccctg tccttcctgc ggaaagcggt tcatcgatga
      541 ggtctttgcc gatatatacc gggcaatggc cgccgaaggc gacctttcgg ggaccgaacc
      601 ccttgccgcc gccggaacac cgctcgttca tcccgggttt gctatgcgaa ggccccccta
      661 cctcccgccc cggtcgctcg tcctcctctc ccggtcgatc ggagagcagg cggcggcccg
      721 cctggtcgcc accgtcccgg aggtcagagg cgtcatccgg gcggggaccg ggacaccggg
      781 gatcagggat accgataccg aaccggagac aaataccctg cttgccggat gcgacgtccg
      841 ggctgacgtc ttctccaccc gggcaggacc cgtcgtcatc tataaacagc agtcggccct
      901 gcacgtcgag ttcccccggg accgcgacga gaagatcctc tccctcgagc gggagatcgg
      961 gcggcaccgc ccccggacat tcgtcgacgc ctgctccggc gccggcaccc tcgcccttgc
     1021 cgccgcccgg gcgggcatcc cccgcgtgat agcaaacgac gcctggtacg cggccgccta
     1081 ctggaccgct tgtaacctcc aggtgaaccg cgagcacctc ggtatcgagg gcgtgacgat
     1141 gcaccggtct tacgacgacc tccggcgccg tgaggtcgcg cgggaacccc tgagggtcgc
     1201 caccgccgcc ggcgcacggg aggtggaggt ctaccagggc gacctccgcc tgctctcgac
     1261 cgttctcccg ccgggcatcg accttaccgc cattgacctc tttgagaagg cggatgctga
     1321 aaaaaccgac aggatcgtcc gggcatggcg ggcccgggtg ggcggagcta tttttatccc
     1381 gtagatcaca gtatatacgg ggactagccc gggtggctcg gcgtcaccta tcacccgaaa
     1441 ccgtcgtcat gcggggggcg aagtctgagg aaggccatag cggcctgcag gatcctctgc
     1501 gtcccgacgg agaagcctcg tcccatgagg tcggcggaga ggtatcaaag ccccggaggg
     1561 ggcggcgacc tcttgccgcg ggtaccggtt caggcccgga agggagcaga cttaccgtgg
     1621 acgttcggcg cccatggggt tgcggggtgg aggagggatg cagcggtgaa tgctggtgcg
     1681 cacggtcgac cgaggacacg tccccaacgg cgatctccat ggcaaaagta acgattcttg
     1741 gggctaccgg gaacgtcggc atgtttgcgg cccgtaccgt atccgagatc ccgcacgtca
     1801 gcgagatgct gctcgtcggg aggccggggc gcgaagattt tcttgcaggc tgctgccgcg
     1861 acctttctga ttcgtttgcg gcgcgaggca cccacgtcag gctctcgtac agcacccgcc
     1921 ttaccgatgt ggaaggctcg gacatcgtca tctgcacggc aggagtccct cgccggcccg
     1981 gccaggaccg aaacgacctc gcctttgaga acgcgaagat cgttgccggg agcgccgaga
     2041 ccatcggccg gtcttcgccg gacgctattc tcttcatggt cacgaacccc gtcgacgtca
     2101 tgactgccgt cgccctgaaa tattcggggc ttcagtcgcg gcaggtcttc ggcctcggga
     2161 cacacctcga ctcgatgcgc ttaaaatccc ttattgccca ctacttccgt gtccacgtca
     2221 gcgaagtgca cacccgcatc atcggcgagc acggagagag catggtcccg ctctggtcgg
     2281 cgacgacgat cggcggcatc cggatcagca atctgcccac cttctcgggg ctgcccgcac
     2341 aggagatgat cggcgcggtc aggacaagcg gcgagacgat catccgggag aagggctcca
     2401 ccgtctacgg acccggagag gcgatcgcaa cccttgtgcg aacgatcctc ggcgatgaga
     2461 accggatcct caccgtctca agctacataa cgagcgagat ccacgggatc ggcgacgtct
     2521 gcatcggcgt acccgcccga ctcaaccgga gcggcgcctt cccggtgccc ataaggctcg
     2581 aggagaacga ggttgccggg tttgaggagt cggtcaagaa gatccgaaaa gtcaccgccg
     2641 acgtgatgga acggctcgga gaggcccatt agggcggaaa agtgggttgg gaagtccact
     2701 tatacaagtg tcgattcaat gatctcggcg ctgccgacac cctcaaggtt ctggagcgct
     2761 gattcgacct catcgggagc cccggctttg tcggggacga cgacggcgat cttcagcgcc
     2821 acaagcccga agccgatcgg ctcttccctt atatcgttga ccggaacggc ccccctgacc
     2881 gcggctttca acgcttcacg gtcgacgtcg ggagactcgg gcatgatctt taagatgatg
     2941 gcgacgtttc ccatcggtta cggcccccga aacccgcatt tttggcatgt gtaggctatg
     3001 ctctgctccc tgcaccggta gcacctgttg atctcgtgcg cgcataccgg gcacgggaac
     3061 ccggtggagc cttcctcggc cagcgtggcg ttgcaggagg tacatcgatc tctcgctgtc
     3121 atcgtggttt cctcaaaagt taacctatca tatcgccccc ggaacaatta aaatctcgga
     3181 tcgatgagag tcccggtgac ggcctcatcc cggagaaccc cgcggacccg gtcgtcgtgc
     3241 ctgccgttga ccacccggac cacgatatcg tgctcaaaga caaacccgat gaacgccgga
     3301 tcgacctcat cggcggtgag ggacggatcc tcgacccggg agaggagacg ccggcggtgg
     3361 tggatgccgt cgaccgactt gaggaggatg aggtcggtcg agagttcatc tgcaacccag
     3421 gcggcgatgg tgtcggaggt gacgttccag gagtggggaa ggggatcggc ttttcgcagg
     3481 gcgcagtagg gaaggaggac cgtcgcttcc gcggggagga cggggctggc gaccgccggg
     3541 atgccgtgcg aggcgatgta ccacccgaac tgctccatac cggcgatcgc catccagtga
     3601 gcggatgtat cggagacggc aaggcgccgg acgaggtcgg cgaacttccc gccgccgggg
     3661 acgatcagca ccggacggcc gacttcccgg agtatgccga gcagcggggc tacccggtca
     3721 aagaggctgc cgcccacctt gacgacgagc gggggcgcgg gcgttgtcat atcgtttgct
     3781 ctttatccgg ataatacata accttgcgta tgcgacggat cgctgcgatc atcctgctcc
     3841 tctgtctcct tgccggcacg gccggcggta tcctgatcgt cgagttctgc cctgacccct
     3901 accggcccgg tgatcccgac gagtacctgg tgctcgaggg cgaaggatcg cttgataggt
     3961 tcgtcgtctc ggacggcaag ggcggttttc ggttcccgcc ggggacctgg atcaacgggc
     4021 gtctggtgat cgcaagaaac agtctcgcgt ttgagcagac gcacggctac ctgcccgact
     4081 tcgagatcga gaagcggaca gcggccgtgc cggacgtcac ctcgagcggg aacctcctga
     4141 tggcgaacca ggccgacgcg ctctacctct accat
//
LOCUS       DAOSII010000150         4167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_18837_length_4167_cov_43.181663, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000150 DAOSII010000000
VERSION     DAOSII010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18837_length_4167_cov_43.181663"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PLV90_09180"
     CDS             complement(<1..325)
                     /locus_tag="PLV90_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOF97324.1"
                     /translation="MVEAQRSRQPGYLRLSGSGELEERAHRAHEVLRDCTICPQGCHV
                     NRIEGELGFCRTGLLPMVASYSPHFGEEAPLVGRNGSGTIFFAGCNMRCEFCQNYTIS
                     QCGAGT"
     gene            521..880
                     /locus_tag="PLV90_09185"
     CDS             521..880
                     /locus_tag="PLV90_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97325.1"
                     /translation="MSGEHNRARDRLPRRISIVAPAIPGAVAWKKGISSPIRVAGMPG
                     TIDLHQAAHNSPEVEQTVIDYPEAFVTAGRDPSQGILREHADTAGWIGIHDRDSVVDR
                     NTHRQLRNLTGSCGVPY"
     gene            864..1439
                     /locus_tag="PLV90_09190"
     CDS             864..1439
                     /locus_tag="PLV90_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin"
                     /protein_id="HOF97326.1"
                     /translation="MGCRIRARPPGAVKEAPKLLRGRTLDERSLSHTHAALLMVAITV
                     ILAILLLLILLGMIPSWSWAGPELPPIIITEIVHTNDDTGILTYASRVFLLNNGSTTY
                     ENDCLRAVFYLDGTRVRTVYTLNGYRLIPSHHYGVRYIKGAGCRSPYWNPGEEMEIDL
                     ADKTLIPGGMVTVEIVNRVTGQVISKHTAQA"
     gene            1485..1931
                     /locus_tag="PLV90_09195"
     CDS             1485..1931
                     /locus_tag="PLV90_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOF97327.1"
                     /translation="MFKTILVAVDGSDISRRALEEALAVARAMQASLHAVHVVQTGAY
                     PILTSNELEPPDIARQALLDSLEREGDRILAETEGQAAAAGIQITVHKHRGHPGASIT
                     ALAQELGADLTVVGSHGRGRIDRFFLGSVSSYVADHATSTVMIVRG"
     gene            complement(1921..2502)
                     /locus_tag="PLV90_09200"
     CDS             complement(1921..2502)
                     /locus_tag="PLV90_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF99 family protein"
                     /protein_id="HOF97328.1"
                     /translation="MHAAKPGLRALGIAESYSGRKRSTLAGVVMRKDLLIDGVAFARV
                     TVGGSDATDAVVRLVADLARRDINFIMISGCVIAWYNIIDPAAVQEATGLPVIAATYE
                     ESEGLEDDIRHHFPDDPGRLAAYRRLGGRTPIRLHTGYTLFIRPCGLSPGDAARLCND
                     FTHEGRVPEPLRVARLVARGIVRSALCGGVVSP"
     gene            2542..3468
                     /gene="aglJ"
                     /locus_tag="PLV90_09205"
     CDS             2542..3468
                     /gene="aglJ"
                     /locus_tag="PLV90_09205"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer glycoprotein N-glycosyltransferase AglJ"
                     /protein_id="HOF97329.1"
                     /translation="MNIERDEVCIFIPTLNEAPTIAELIGEFKERGFRHILVMDGHST
                     DGTPDIARAAGAVVRTQTGRGKGNAIIEAVAIIDKPYVLMLDGDGTYAPDDAERMLDP
                     LGLGFDHVIGDRLAGPEGEAFTRLNLLGNQILNLMFKLAHGRDLHDILSGYRAFTLPS
                     IRQMTLKESGFEIETEMAVEAVRHGQRITVVPVRYLARPGTATKLNPLQDGARIFSTI
                     YRLAKMNNPIFYFGIIGLFISLAGGVIGVYVVLEWLRNIEHLPLTILTVLLITIGFQI
                     FMFGVISDMLLGFHRETTRQIEQLQGASKPPR"
     gene            complement(3451..3981)
                     /locus_tag="PLV90_09210"
     CDS             complement(3451..3981)
                     /locus_tag="PLV90_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HOF97330.1"
                     /translation="MLDQEFERIGKRLFLEGLVGANFGNMSVRDAGGFFITRTGAYLD
                     AREVPVHVPDTGDAPREASSEYRVHRAVYRETPHRAIVHAHPVHAVAASLDTDIIRPV
                     DSEGGMLCPAIPVVAGDPGTDEIAENVAGALRGGHIVIVRGHGTFTAGKTLDEAYIYT
                     SVAEYACRILLLSGRL"
BASE COUNT          792 a         1345 c         1270 g          760 t
ORIGIN      
        1 ccgtgccggc gccgcactgg ctgatcgtgt agttctggca gaactcacag cgcatgttgc
       61 agcccgcaaa gaatatcgtt cccgacccgt tcctccccac gagcggcgcc tcctcgccga
      121 agtgcgggct gtaacttgca accatcggga ggaggccggt ccggcagaac cccaattcgc
      181 cttcaatacg gttcacatgg cacccctggg ggcagatggt gcagtcacgg agcacctcgt
      241 gcgcccggtg cgcccgctcc tccagttcgc cgctcccgga taggcgcagg tacccgggct
      301 gccgggatcg ttgtgcttca accatgatct cctcctttgc gtgagacaaa agaaggttta
      361 ccctgcgtct gcaggcctcg ccctgcggcg ggcgaagagt acactccggc gccggcccgg
      421 aaccacggcc cgtttcgaat attatttcct gatttcccgg ccaggaaaag gactattttg
      481 acaatactta tatcctgcgt gaccgattct ccattaccgt atgtccggcg aacacaaccg
      541 tgcgcgcgac cgcctgccgc gcagaatctc gatcgtcgct cccgccatcc ccggtgcagt
      601 cgcctggaag aaagggatct cttctccgat cagggtcgcc gggatgccgg gtacgatcga
      661 tctccatcag gctgcccaca atagcccgga agtagagcaa acggtcatcg attacccgga
      721 ggccttcgtc acagcgggca gggatccttc tcaagggatc ttgcgggagc atgccgacac
      781 cgccgggtgg atcgggatcc atgatcggga tagcgtggta gaccgaaaca cccacaggca
      841 gctccggaac ctgaccgggt cgtgtggggt gccgtattag ggcccggccg ccaggtgccg
      901 taaaggaggc accgaaactc ctgagagggc gcaccctgga tgagcgcagc ctctcgcaca
      961 cccacgccgc gctcctgatg gtcgccatca cggtcatcct tgccatcctc ctcctcctga
     1021 tcctgctcgg gatgatcccc tcgtggtcgt gggcggggcc tgaactgccg ccgatcatta
     1081 tcactgagat cgtccacaca aacgatgata ccggaatact gacctatgca agcagggtat
     1141 tcctcctcaa caacggcagc acgacctacg agaacgattg cctgagggcg gttttttacc
     1201 tggacggcac gagagtccgc accgtctata ccctgaacgg ttaccgcctc ataccatcgc
     1261 accattatgg ggtgcgctac atcaaaggtg cggggtgccg ctccccatac tggaatcccg
     1321 gcgaggagat ggagatcgat cttgccgata aaaccctcat cccgggcggg atggtcaccg
     1381 tggagatcgt gaatagagtg acggggcagg tgatatcgaa gcacaccgcc caggcatgac
     1441 tgcagagaca tctatttgta ctggcacgct tctaaaagag aagtatgttc aagacgattc
     1501 tcgtggcggt cgatgggtcg gatatcagcc gtcgtgcact cgaggaggcg ctggcagtcg
     1561 cccgtgcgat gcaggcatca ctccacgccg tgcacgtcgt ccagacggga gcgtacccca
     1621 tactgacctc gaacgagctc gagcctcccg atatcgcgcg gcaggcgctc ctcgattcgc
     1681 tcgaacggga gggagacagg atccttgccg agacggaagg gcaggcggcc gctgccggca
     1741 tccagataac cgtccataag cacaggggcc atcccggggc cagtataact gcactggcgc
     1801 aggaactcgg cgccgacctg accgttgtcg gatcgcacgg ccgcggccgg atcgatcgct
     1861 tcttcctcgg gagcgtcagt tcctacgtcg ccgaccacgc gacgtcgacg gtcatgatcg
     1921 tcaggggctg acaaccccgc cgcagagggc cgaccggacg atgccgcggg cgacgagccg
     1981 cgccactctg agcggttcgg gcacccgccc ttcgtgcgtg aagtcgttgc agagccgtgc
     2041 tgcatccccg ggagagaggc cgcaggggcg gatgaagagc gtgtacccgg tatggagccg
     2101 gataggcgtc cggccaccga gccgccggta tgcagcgagt cggccgggat cgtccgggaa
     2161 gtggtggcgg atatcatcct cgagcccctc cgactcctcg taagtggcgg cgatgacggg
     2221 taagccggtc gcctcctgca ccgccgccgg atcgatgatg ttgtaccagg cgatgacgca
     2281 accgctgatc atgatgaagt tgatgtcccg gcgtgcgaga tcggcgacaa gacggacgac
     2341 cgcatcggtc gcatccgacc ccccgacggt cacccgggcg aaggctactc cgtcgatgag
     2401 gaggtcttta cgcatcacga cgccggcaag ggtggatcgc ttccgacccg agtagctctc
     2461 ggcgataccg agagcacgaa gccccggttt ggctgcgtgc atgcgaagtt cttatgttgc
     2521 tcaatcggat atagtacacc gatgaacatc gagcgagatg aggtctgtat ctttattccc
     2581 acattaaatg aggccccgac gattgccgag ctgatcgggg agtttaagga acggggtttt
     2641 cgccacatcc tcgtcatgga cgggcacagc accgatggga cgcctgatat cgcccgagct
     2701 gccggggcgg tcgttcggac gcagacgggc agggggaagg gcaacgccat aatcgaggcc
     2761 gtggcgatca tcgataagcc ctacgtgctc atgctcgacg gcgacggcac ctacgcgccg
     2821 gatgatgcgg aacggatgct cgacccgctc ggcctcgggt tcgaccacgt catcggggac
     2881 cgtctcgccg gccccgaggg ggaggcgttc acccggttaa acctccttgg caaccagata
     2941 ctcaacctca tgttcaagct cgcacacggg agagacctcc acgacatcct ctccggctac
     3001 cgggccttta ccctcccctc catccggcag atgaccctca aagagtcggg gttcgagatc
     3061 gagacggaga tggcggtcga ggcggtgagg cacgggcagc ggatcacggt cgtcccggtc
     3121 cggtacctcg cccggcccgg caccgccacc aagttaaatc ccctccagga cggtgcgagg
     3181 atcttctcga ccatataccg gcttgcgaag atgaacaacc ccatatttta cttcgggatc
     3241 atcggtctct tcatatcgct tgccgggggc gtcatcggtg tttacgtcgt ccttgaatgg
     3301 ctcaggaaca tcgagcacct gccgctcacc atcctcacgg tcctcctgat caccataggg
     3361 tttcagatct tcatgttcgg ggtcatcagc gatatgctgc tcgggttcca ccgcgagact
     3421 acccgccaga tcgagcagtt gcagggcgcc tcaaagccgc cccgataaca agaggatccg
     3481 gcaggcgtac tcggcaaccg acgtgtagat gtaggcctca tcgagcgtct ttccggcggt
     3541 aaacgtcccg tgtccccgaa ctatgacgat atggccgccg cggagcgctc cagcgacgtt
     3601 ctctgcgatc tcgtcggtcc cgggatctcc tgcgacgacc gggattgccg gacagagcat
     3661 cccgccttcg ctgtcgaccg gccgaatgat atcggtgtcg agcgaggcgg cgacggcgtg
     3721 gacggggtgg gcatggacga tcgcgcggtg cggcgtctcc cggtagaccg cccggtggac
     3781 ccggtactcg cttgaggcct cgcgcggggc gtctcctgta tccggcacgt gaaccggcac
     3841 ctcccgtgcg tcgaggtacg cgccggtccg ggtgatgaag aacccgcccg catcgcgtac
     3901 actcatgttg ccaaaatttg ccccgacaag cccctcgaga aagagacgtt ttccgatccg
     3961 ttcaaactcc tggtcaagca tcttctgcac ccggacttac aggatacgtt ggcgtgccgg
     4021 gggatatgtg catgcccccg tcgggggccg gcgccgggcc ctctccgcgc ccgcgccttt
     4081 acaggaggat gcccgtgctc ctctccggca cccccaccct gccgactctt ctgcgaagga
     4141 tacccgcacg cttctgccgg cgacccc
//
LOCUS       DAOSII010000151         4158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_18898_length_4158_cov_46.628564, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000151 DAOSII010000000
VERSION     DAOSII010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18898_length_4158_cov_46.628564"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PLV90_09215"
     CDS             complement(<1..581)
                     /locus_tag="PLV90_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOF97331.1"
                     /translation="MKLITVAGPPSSGKTSVILKTIAALGLPEGSVGVVKFDSLTSFD
                     HQRYDEQQIPNLTAFAGKICPDHFFVSNVERAVEWGMKKGLSVLITESAGLCNRCSPY
                     VNAILAVCVIDNLSGVNTPRKIGPMLKYADIVVVTKGDIVSQAEREVFAFNIREVNAS
                     ATVLFVNGITGQGAFMLARYCLDALDIQTLRDR"
     gene            1289..1519
                     /locus_tag="PLV90_09220"
     CDS             1289..1519
                     /locus_tag="PLV90_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97332.1"
                     /translation="MATTIQLQPETKSRLDTLKTHPRESYDETLNRIMDAFFDPEPLS
                     EEILQEIEESIADMRAGIRGRPFEDYVRERGI"
     gene            1519..1791
                     /locus_tag="PLV90_09225"
     CDS             1519..1791
                     /locus_tag="PLV90_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOF97333.1"
                     /translation="MTWRLLLMPRAEQKLNKIPDHDATRIKDELRALANEPYPRLYAK
                     KLKGHPNSPLYSFRVGQYRIILVFEGNVMIITVIDIGNRSNVYRKY"
     gene            complement(1968..4049)
                     /locus_tag="PLV90_09230"
     CDS             complement(1968..4049)
                     /locus_tag="PLV90_09230"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOF97334.1"
                     /translation="MTRTAHLFLIILILAVALLPVSAMAEAGTTELRIVRYASDNRTV
                     LDETTVDYLWMKANLPVYGDGRTHYFHEGPVLEEYWETTHPGEEYDPWDPAEDVQGSV
                     LQKGDLGAVMGTGVTDLCALIGGAEEGDRIAVTCRDGWRKTFPYEYIYRPDPRQGPLV
                     ICWFNGDEAPGEKQGVGYPDTTYLSGMRMAFFADTSSNPWGWHIFGNNDMRECWDEEY
                     WNYGAQYPSAVGVSGKWVSEIGIFTDRLPPVPVAEFETNVTSGRLPLAVAFNDTSTAN
                     PRTWTWNFGDGSSSAEKNPVHVYTTAGDYTVTLTVSNVAGTASVIRAGAVTVENAVVP
                     TADFAANATSGEAPLAVAFTDASDGKPAEWEWDFGDGEGATGPAPVHVYSSPGTYTVA
                     LTATNHKGSDTITKTGAITVDVPVPAPPIASFSTDVRAGDAPFEAAFTDTSQKFPTTW
                     AWDFGDGASSEEPNPVHIYSSPGVYTVALTVTNDLGSDMVTRPQFIAATENGKAILFR
                     GTVPLGEGTFSVKAPSGQAYTPNKRTPMGALDAVAEMRGFTYVIGDKKYSESRLLFLD
                     GVGEYLLEKSTGKTWVCFHNGELLDDYGRPSTDAFNRRPVADGDTVLFWYGDYFIYST
                     GKMEPRGGGVCLDNTLAIVEMTIASEPAAGGATAAQAGAASTSAPAPESPPGVWLPIL
                     AIGLFLVLRRV"
BASE COUNT          826 a         1296 c         1211 g          825 t
ORIGIN      
        1 ttgcggtcgc gaagtgtctg gatgtcaagg gcatccagac agtagcgggc aagcatgaac
       61 gctccctggc cggtgatccc gttgacgaag agaacggttg ccgatgcatt gacttcacgg
      121 atgttgaagg caaagacctc gcgttccgcc tgggagacga tatctccctt ggtgacgact
      181 acgatatcgg catacttcag catcggcccg atctttctcg gcgtgttgac gccggagaga
      241 ttgtcgatga cgcataccgc caggatggcg ttcacgtacg gcgagcatcg gttgcagagg
      301 cctgcgcttt ctgtaataag aaccgataat cccttcttca tgccccattc gactgccctt
      361 tcgacattgc tgacgaagaa gtgatccggg cagatctttc cggcaaacgc agtcagattg
      421 ggtatctgtt gttcgtcgta acgctggtga tcgaaagagg tgagcgagtc gaacttcacg
      481 acccccacgc tcccttcggg cagcccgagg gctgcgatgg ttttgagaat tacagaggtc
      541 tttcccgaag acggagggcc tgcaacggta atcagcttca tagcaaccgg ttttttgcta
      601 tggggtgcgg gaatgtataa tgctttctat aaacaatctc tttaagttaa tgagatataa
      661 ttaaaatcat gattgtttac atagtttttc cggtttcgat tgcatcaacg cccggatgaa
      721 atgatgcacc atgggcacag tcggcaaaag actgtttctc ccgatcactg ctcgccgaag
      781 agtccaaaat ttttgtaatc cacgtaaaag gggtggcgcc cggttcacct cttaaggtct
      841 cccgtttttc ggtaaaaagg agatcagatc cttcgcaaca ccaggaagag cccgattgcg
      901 aggatgggga gccacacacc cgggggcgac tgcggcgccg gcgtgctcgt gctcgcggcc
      961 ccggccccac tcaggaccgg aaccagcccc actcgccggg cgggtgccgg gactcccggc
     1021 gcgcgcggac gtcccgcaag ggcacccggc ggagtccagc ggaatcaatg ggcgctgatc
     1081 ctccgggccg acgtttcgcc cccgtcttca ggaaaaggcc gccgaacccg cccacgcagg
     1141 gcaccgatgg aggggtccgc gaaaataacc ggatctgtga acccttctgc gatgctggcg
     1201 gaatgggccg tggatcctcg gactggttcg atgatctgaa cggaaccctg acaaaatcat
     1261 tatactttat ggtaacctat gggtagttat ggcaacgacg atccagctcc aacccgagac
     1321 caagtcccgc ctggataccc tgaagacaca tcctcgggag agttacgacg agacgctcaa
     1381 ccgcatcatg gacgcgttct tcgatccgga gcctttgagc gaagaaatac tgcaggagat
     1441 cgaagaaagc atcgcagaca tgcgggccgg tatcaggggt cgcccctttg aagattatgt
     1501 gcgggaacga ggcatctgat gacctggcgg ctcctcctca tgccaagggc cgagcagaaa
     1561 ctcaataaaa tcccggacca cgatgccaca cgaatcaagg acgaactccg cgccctggca
     1621 aacgagccgt accctcgcct gtacgccaaa aaactgaaag gacacccgaa cagtccgttg
     1681 tactcttttc gtgtgggaca gtatcggatc attcttgtct tcgagggcaa tgtgatgatt
     1741 attaccgtga tcgatattgg aaaccggagt aacgtctatc ggaaatattg atgtgtcgcg
     1801 agaactgcga aaccgatagt caatcccaac tacggtgaag tgctttacag gcagcgtggt
     1861 gaccacaccg ggtgcgaagg cgagcgggat ctcacgggtg ctcgtggtgt agatccggac
     1921 cccggctcac cccgcaaagt ccccctctct ccagtaaaaa agggatatca gacccttcgc
     1981 aacaccagga agagcccgat tgcgaggatg gggagccata cgcccggggg cgactccggc
     2041 gccggcgcgc tcgtgctcgc ggccccggcc tgcgccgcgg tcgccccacc tgccgcgggc
     2101 tccgacgcga tcgtcatctc gacgatcgcc agcgtattgt caagacacac tccccctccc
     2161 ctgggttcca tcttccccgt gctatagatg aagtagtcac cgtaccagaa gagcaccgta
     2221 tctccatccg cgaccggccg ccggttgaag gcatcggtcg agggcctgcc gtagtcgtcg
     2281 agcagctcgc cgttatggaa acagacccag gtcttcccgg tcgacttttc gagcaggtac
     2341 tcgcccaccc cgtcgaggaa gaggagcctg ctctccgagt acttcttgtc cccgatcacg
     2401 taggtgaacc cccgcatctc tgccacggca tccagcgcgc ccatcggggt gcgcttgttc
     2461 ggggtgtagg cctgtcctga cggcgctttg accgaaaacg tcccctcgcc gagcggcacc
     2521 gtcccgcgga agaggatcgc cttcccgttc tccgtcgccg cgatgaactg tggtctcgtt
     2581 accatatcgc tgccgaggtc gtttgtcacc gtgagcgcga ccgtgtagac ccccggtgac
     2641 gaatagatat ggaccgggtt tggctcctct gaagaggcac cgtccccgaa atcccaggcc
     2701 cacgttgtcg gaaacttctg cgaggtgtcg gtgaacgcgg cctcgaacgg agcatccccg
     2761 gccctcacat cggtggaaaa cgacgctatc ggcggtgcgg gcaccggcac gtcgacggtg
     2821 atcgcaccgg tcttcgtgat cgtatcggac cccttgtggt tcgtcgccgt gagcgcgacc
     2881 gtgtaggtcc ctggcgacga atagacgtga accggggccg gcccggttgc accttcccca
     2941 tcgccgaagt cccactccca ctccgccggt tttccgtcgg aggcatcggt gaacgcgacc
     3001 gcgagcggag cctcccccga tgtcgcgttc gcagcgaagt ccgcggtcgg aaccactgcg
     3061 ttctcgacgg tgacggcgcc ggcccggatc acgctcgcgg tcccggcgac gtttgaaacc
     3121 gtcagggtga cggtgtagtc cccggccgtg gtgtagacgt ggaccgggtt cttctccgcg
     3181 gacgaagacc cgtcgccgaa gttccaggtc cacgtcctcg ggttggcggt ggacgtatcg
     3241 ttgaacgcga cggccagcgg cagcctcccg gacgtcacgt tcgtctcaaa ctccgcgacg
     3301 ggcaccgggg gcagccggtc ggtgaagatc ccgatctcgc tgacccattt ccccgagact
     3361 ccgacagccg aggggtattg cgccccgtag ttccagtact cctcgtccca gcactcccgc
     3421 atatcgttgt tcccgaagat atgccatccc caggggttcg aggaggtgtc cgcgaagaag
     3481 gccatgcgca tccccgacaa gtaggtggtg tccgggtacc cgaccccctg cttctcgccg
     3541 ggagcctcgt ccccgttgaa ccagcagatc accagcggac cctgccgcgg gtcggggcgg
     3601 taaatatact cgtaggggaa ggtcttccgc cacccgtcgc ggcaggtgac ggcgatccgg
     3661 tccccctcct ccgccccgcc gataagagcg cagaggtccg taacaccggt ccccatcacg
     3721 gccccgaggt cgcctttctg cagcacgctc ccctgcacgt cctcggccgg atcccaggga
     3781 tcgtactcct cgccgggatg ggtggtctcc cagtactcct cgagcaccgg cccttcgtgg
     3841 aagtaatgcg tcctcccgtc cccgtaaacg ggaaggttcg ccttcatcca gaggtagtcg
     3901 acggtcgttt cgtcgaggac ggttcggttg tcgctcgcgt acctcacgat acgcagctcg
     3961 gtagtccccg cctccgccat cgccgatacc ggcagcagcg cgactgccag tatcaggatt
     4021 atcaggaaca gatgcgccgt tcgtgtcatg aaatcatccc aggctccctt cgggcgcctg
     4081 gagggcgctc gccggcccgt aaacgatttt agtttacttt ttatcaaaag aaatatgttt
     4141 ccatttactt ccggagcg
//
LOCUS       DAOSII010000152         4154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_18921_length_4154_cov_33.453525, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000152 DAOSII010000000
VERSION     DAOSII010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18921_length_4154_cov_33.453525"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..1473
                     /locus_tag="PLV90_09235"
     CDS             76..1473
                     /locus_tag="PLV90_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOF97335.1"
                     /translation="MVKRRLIIALLVSCLLLCCTAQAVTLRISVADEKTGSALVDASI
                     YVDGDYIGSTASDGTYSYVHSGKKDLRLKVVRSGYRDWLDYVGADRTGIVVNMARKDE
                     ILTFELYDAGTLRPVVGALVRIEGDGVSSSGTSGSGGSTTFTVRAGALYNVEVRASGY
                     YDLSKTVQMESSDRLVQYWLFSSDLLAVQVRDGATSVPIEGAEVFVDGSRAGATDVDG
                     RLPLHLQREKRYSLRITAPDYQPYQEERYLGAGDVFLPVHLSKSAYPVSVTAFCEAMK
                     PVEGAEVSLNGTLQGKTNQYGRLALSDIHAGTYELLVRAPGYEDWSVTRQISGKGEDI
                     VADLKYDRASVTVRVEGPDRKAVAGAVIIIDGRTAGVTDSLGCFKTALTTNRVYAVAA
                     ARDGYQNVSVDADIPLGTAEFTVPLVMEQAVNVWVLVAGIGVVTAVILGAIFMVRRRQ
                     AGQSRSRSPRGRDGL"
     gene            2066..>4154
                     /locus_tag="PLV90_09240"
     CDS             2066..>4154
                     /locus_tag="PLV90_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOF97336.1"
                     /translation="MKESIDLQIDHTIPHAIAGLWYKPEVTAGGEVDITVRMEDRYGN
                     VVDSRREDALGISLQAENVTFMVGSPGGGAAFAGDRDKISVPVDESGNATARLRVDTV
                     AGENVVYIQPPKPIKGEYISITGTADGMPAGIACMAIPHDASAPANGESKVTLMYTLT
                     DAYGNPAAGQDLWVNSSGLRGDSKLISSSSRGEVWITYGPEDNTGRIAITATAAANPS
                     VTITTWVEFTSTEPVDMLLTASPQTMASRDRNPDVASELRAKVMDIRGNPVRGETVTF
                     EIVSNRSAPFNQTMDQGLEAASAITDADGCAVVRFQPGGFTNDRKAPGWDASATGNAV
                     VRATWENTTRDCHVTREINLTWMNYPYLSVETEVSRATISVNSTDGISNTTDVTIRLK
                     GDGYAMRPDPIDVVLVIDRSGSMTGTDVSPTRMKAAQNAAKDFVGNMNLATEDGDRVA
                     LVSFAFDARLDQELTCNSNKIKGVIDRLTPDGATNMRLAYYTAVKYLKENGRPEAVKA
                     VILMGDGDWNYHGSPLAKGIGYADNNRYLTSDWYSSPYWAPLSGYRWSGSSYNFSNEM
                     YEWYHDLPSPEGNANVERELGKGKWYDTIGKEWVEKHSLTSLTCNNGQFTNQNMSVYA
                     NSGDDIEKVRIYSIGFASKLNSNVEKDLGILSSATGGKYVWAGNETELKKIYTDIAGE
                     LKTEAGVDTDMTIF"
BASE COUNT          890 a         1121 c         1332 g          811 t
ORIGIN      
        1 gtccttgagc gtgttccgcc cgagcgctgg gtgtaaaccg atgcactcaa atttttgggc
       61 gcagtacact ccagcatggt aaaacgtcgt ctcatcatag cccttcttgt ctcctgcctc
      121 ctcctctgct gcacggcgca ggccgtcacc cttcggatca gcgtggccga cgagaagacc
      181 ggtagcgcgc tcgtcgacgc ctccatctac gtcgacggcg actacatcgg gagcactgca
      241 tcggatggca cctactccta cgtccattcc gggaagaaag acctgcggtt gaaggtggtg
      301 cggtcgggct accgggactg gctggattac gtcggtgccg ataggaccgg cattgtagtc
      361 aacatggccc gcaaagacga gatcctcacg ttcgagctct acgatgcggg aaccctgcga
      421 ccggtcgtcg gggccctggt gcgtatcgag ggcgacgggg tatcgagctc ggggacgagc
      481 gggagcggcg ggagcacgac cttcacggtg agggcgggtg cgctctacaa cgtcgaggtc
      541 cgcgcgtcag gctactacga tctctcaaag accgtgcaga tggagagcag cgataggctc
      601 gtccagtact ggctcttctc aagcgacctt ctcgcggtcc aggtgcggga cggcgcgaca
      661 tccgtcccca tcgagggtgc ggaggtcttc gtcgacggtt cgcgcgccgg agcgaccgat
      721 gtcgacggca gactgccact ccacctgcag cgggagaagc ggtattctct caggattacg
      781 gcaccggatt accagcccta ccaggaggag cgttacctcg gcgcgggcga tgtcttcctc
      841 ccggtgcacc tctcgaagtc ggcatacccg gtctcggtaa cggccttttg cgaggcgatg
      901 aaaccggtcg agggtgcgga ggtctccctc aacgggacgc tccaggggaa gaccaaccag
      961 tatggccggt tggcgctctc cgatatccac gcgggcacgt atgaactcct ggttcgggcg
     1021 cccggctacg aggactggag tgttacacgc cagatatccg ggaaagggga ggacatcgtc
     1081 gccgatctga agtatgaccg ggcgagcgtg accgtccgcg tggaggggcc cgaccggaag
     1141 gcggtcgccg gtgcggtcat catcatcgac ggtcggaccg ccggggtgac cgacagcctc
     1201 gggtgtttca agacggcgct cacgacgaac agggtgtatg ccgtcgccgc cgcccgcgac
     1261 ggttaccaga acgtctcggt cgatgctgat ataccgctcg gtaccgccga gttcacggtt
     1321 ccgctcgtga tggagcaggc cgtcaacgtc tgggtgcttg tggccggcat cggggtcgtt
     1381 acggccgtca ttctcggtgc gatcttcatg gtgcggcgcc ggcaggccgg gcagagtcgg
     1441 agccggtcgc cacggggccg ggacgggtta taggtctccc cggcccctcc ccggaagtac
     1501 tttttttccc ggggtccggc gacaggatgt atccccggaa gaaaaaataa tttcagatac
     1561 aggataaagt aatatctctt ttctcacatg gggtcctttc atcgaacaaa tattcaaaca
     1621 gcgtgaatat cattgcccta ttcgcaaaca gagccttctg gcttgtttat catattagta
     1681 atatttatat actttgttgt gataggagca gatacggtga agtatgaaac tacagacata
     1741 tgcgtacctt gccgtcgggc tgctccttct tgcggccccg gccggggcgt taatacctga
     1801 taacatcacg ataagcgcga gccccgtgtg gttgacggcg gggggcgaca atgccgcaac
     1861 catcacggtg caggtcaaga acagcacctc cccgctcgag ggagtcggtg tcgacttcga
     1921 tgtggatggt acgtatggga gcatatctcc cctgcacgcg gtgaccgatt ccgatgggaa
     1981 ggcgatggcc gacttcaggc ccgggaccgt tgccggaacc gcgaagatca ccgcgacggt
     2041 gccgtatgag gggccggatg agccgttgaa ggagagcatc gacctgcaga tcgaccacac
     2101 gataccgcat gcgatcgccg gcctctggta taagccggaa gtgaccgccg ggggggaggt
     2161 ggatatcact gtccggatgg aggaccggta cgggaatgtc gtggatagcc ggcgggagga
     2221 tgccctggga atttctctcc aggcggagaa cgtgaccttc atggtgggtt cgcccggggg
     2281 cggggcggcg tttgccggcg atcgggataa gatctcggtg ccggtggatg agagcgggaa
     2341 tgccaccgca aggctccggg tcgatacggt ggccggggag aacgtcgtct acatccagcc
     2401 gccgaaacct atcaagggcg agtatatctc catcaccggc acggcggacg gtatgccggc
     2461 gggtatcgcc tgcatggcaa ttccgcacga tgcctcggcc ccggcaaacg gggagagcaa
     2521 ggtaacgctg atgtatacgc tgaccgatgc gtacgggaac ccggcagcgg gccaggacct
     2581 ctgggtgaac tcatcaggat tgcgggggga ctcaaagctc atcagctcga gttcccgcgg
     2641 ggaggtgtgg atcacctatg ggcccgagga taacaccggc aggatcgcta tcaccgcaac
     2701 ggccgccgcg aatccgagcg tgaccatcac cacctgggtt gagttcacca gcaccgagcc
     2761 ggtggacatg ctcctcaccg cgagcccgca gacgatggcg agccgggacc gtaaccccga
     2821 tgtcgcatcg gagctccggg ccaaggtgat ggatatcagg ggaaacccgg tccgggggga
     2881 gacggtgacg tttgagatcg tctcgaaccg ctccgcaccc ttcaatcaga ccatggatca
     2941 ggggttggag gctgcatccg ccatcaccga cgccgacggg tgtgccgtcg tcaggttcca
     3001 gccgggcggg ttcacgaacg atcggaaggc gccggggtgg gatgcctctg cgaccggcaa
     3061 tgcggtcgtc cgggcgacgt gggagaatac cacccgggac tgtcacgtta cccgggagat
     3121 taacctgacc tggatgaatt acccgtacct ctccgtcgag accgaggtct cccgtgcgac
     3181 gatctccgtg aacagtacgg atgggatctc caatacaacc gacgtcacca tccggctgaa
     3241 gggggatggg tatgcgatga ggccggatcc gatcgatgtg gtgctggtta tcgatagatc
     3301 ggggagtatg acaggaacgg acgtgagtcc gacgaggatg aaagcagcgc agaatgcagc
     3361 aaaagacttt gttggtaaca tgaatttagc gacagaggat ggagatcgtg ttgcactggt
     3421 ctcgtttgct ttcgacgcca ggctggatca ggagttgact tgtaattcca ataaaattaa
     3481 gggggtgatt gaccgtctca ctcctgatgg tgcgacaaac atgaggctgg catattatac
     3541 cgcggtaaaa taccttaagg aaaatggaag gcctgaagcg gtaaaagcgg tcatcctcat
     3601 gggtgatggt gactggaact accatggttc gccgttggcg aaaggcattg gttacgcaga
     3661 taataaccgt tatctcacca gcgactggta ttcgagccca tattgggcgc ctctatctgg
     3721 atatcgctgg agcgggagca gttacaattt ctcaaatgag atgtatgagt ggtaccacga
     3781 tctcccgagt ccggagggta atgcgaatgt tgagagggaa ctgggtaagg gaaagtggta
     3841 tgatactata ggtaaggagt gggtcgagaa acatagcctc accagcttga cttgcaataa
     3901 tggccagttc accaaccaga acatgtcggt atatgctaat agtggcgatg atatcgaaaa
     3961 agtgagaatt tacagcattg ggttcgcctc gaagttgaat tctaatgtcg agaaagatct
     4021 tggtatcctc tcaagtgcaa ctggcggaaa gtatgtctgg gctggcaacg aaacggaact
     4081 taaaaaaatc tacactgata tcgccggcga actcaagacc gaggccggcg tcgacaccga
     4141 tatgaccatc ttca
//
LOCUS       DAOSII010000153         4148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_18964_length_4148_cov_44.769607, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000153 DAOSII010000000
VERSION     DAOSII010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18964_length_4148_cov_44.769607"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PLV90_09245"
     CDS             complement(<1..537)
                     /locus_tag="PLV90_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HOF97337.1"
                     /translation="MANRVKFGVVGLDEMLMGGLLERSICAIVGTYGTGKTMFALQFA
                     YEGLSQGEGVIYISLEEREELLHATMAQKGWDTKVFGDRFSLLRLDPTDFNLAISSIK
                     SELPALIKSTGATRVIIDPISLFEGLFVDESVRRQEMFGFIEMMRDEACTLVVTSETD
                     IRDPNGSRYGLAEYLADAV"
     gene            complement(538..1374)
                     /locus_tag="PLV90_09250"
     CDS             complement(538..1374)
                     /locus_tag="PLV90_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HOF97338.1"
                     /translation="MHERLRLRVPTGITSLDPLLDGGAPPGSVVLLLGEHGAGNVEFV
                     QTSIMHLATLKRSGKAGEHLLLPGRIVYVTLTRMREDILREMALSFNPDLYTQLEEEI
                     VFYDLSEIYFDASIVPPGWYGEGSTIERLQKKQRRESGDILADVARVLDGCPDNSLLV
                     MDSLTGIAPSLTGSPRWHAFIAFLRGLQQAAKRRNIAIYLLLTSGILDRSQELEIADC
                     VDATLLFRWEETGAQRRQRVMHFERFRGVMPRLEDKDLVKFAVKISAAEGFEVKNIRV
                     VI"
     gene            1640..3283
                     /locus_tag="PLV90_09255"
     CDS             1640..3283
                     /locus_tag="PLV90_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HOF97339.1"
                     /translation="MTQGIPHPAAKEPPSRERGTLSRLLDHSCFFRKVGPAAGRYDFA
                     EHGPLVEAEPPSGYEELDRYWIAAGLSLAVIAKNTRTNQAEYLLFEPVLSEFEYELLE
                     RLFDDLRDVLILDDHDLIADRRVVLSRKAQDLFAEYGLTLDDTSAFKIRYYLERNFLG
                     WSRIDALMKDPRIEDISCDGIRIPLFLYHRRHQNIKTNIFFDEPALNSLAITLAQRSG
                     KHVSIGSPLVDATLPDGSRLQLTFGSEVTTRGTSFTIRKFRETPFTPVELMETKTFDV
                     DQLVYFWMAIENNKSLLFVGGTASGKTTSLNAVALFIPPLAKIVSIEDTREITLYHEN
                     WVATVTRERISEGLGATIGMFDLLKAAMRQRPEYILVGEVRGNEAQTLFQAMNTGHTT
                     FSTMHAGGVDAAIHRLENAPLNVPRNMLQALDIISVQALTHRGYDRARRCREIVEIAG
                     IDPITGSLQVNTVYEYNPVPDTFSYSGRSRILGEIMEYRGWSRTRLDEEMQIRRSVLL
                     AMQNQGIKDYRSVARIIQAFTIEPDRVLASLDDLGRLIR"
     gene            3280..>4148
                     /locus_tag="PLV90_09260"
     CDS             3280..>4148
                     /locus_tag="PLV90_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOF97340.1"
                     /translation="MILPPCIRIRRLIRRLVERDPIRYRSLREDLVAANLGVTLDRYL
                     LLTFLASGLFGAFWAILVFLVLRFAVLPRMSIGIYNVFAIHLPEVMLVDPMISILQVV
                     ASLVVFLITAYAASLVFLQYPTLVKKNRATRINLLLHHAVAYMYAMRQGGAEMMGVFR
                     AIGGNSAVYGEAAHEFQRVIRDTDYFGYDQIAALRHLQETTPSEKLAEFVQDLVSVVE
                     SGGDMLAFLDARVRIYQEEARFEQKTFLSTLQLVAEAYVTLFVAGPLFIIIVMVVMGF
                     MGSTPILQLSVII"
BASE COUNT          795 a         1386 c         1156 g          811 t
ORIGIN      
        1 caccgcatct gccaggtact ccgcaaggcc ataccggctc ccgttcggat cccgtatatc
       61 ggtctcgctc gtcaccacga gggtgcaggc ctcgtcccgc atcatctcga tgaacccgaa
      121 catctcctgc cgccggacgg actcgtccac gaagagccct tcaaagagcg atatcggatc
      181 gatgatcacc ctcgtggccc cggtgctctt gatgagggcg ggaagctcgc tcttgatgct
      241 gctgatagcg aggttgaagt cggtcgggtc cagccggagg agggaaaacc ggtcgccaaa
      301 aaccttcgta tcccatcctt tctgcgccat ggtggcgtgg aggagttcct cccgctcctc
      361 aaggctgata tagatcaccc cctcgccctg gctgagcccc tcatacgcga actgaagggc
      421 aaacatcgtc ttcccggtcc cgtaggtgcc gacgatcgcg cagatgctcc gctcgagaag
      481 ccctcccatg agcatctcat ccaggcccac gaccccgaac ttcacccggt ttgccaccta
      541 tatcaccacc ctgatgttct tcacctcaaa cccttccgcc gcagatatct tcaccgcgaa
      601 cttcacgaga tccttatcct cgaggcgggg catcacgccc cggaacctct caaagtgcat
      661 gacccgctgc cggcgctgtg cgccggtctc ttcccagcga aagaggagcg ttgcgtcgac
      721 acaatcagcg atctcaagtt cctgcgaccg gtccagtatg ccggaggtga gcaggagata
      781 gatcgctatg ttccggcgct ttgctgcctg ctgcagaccg cgcaggaagg cgatgaacgc
      841 atgccaccgc gggctccccg taagcgacgg cgcgatcccg gtcagcgaat ccatgacgag
      901 caggctgttg tccgggcacc cgtcgagcac ccgggcaaca tccgccagga tatcgccgct
      961 ctcccggcgc tgcttcttct gcaggcgctc gatggtgctc ccctccccgt accagcccgg
     1021 ggggacgatg cttgcatcga agtagatctc tgagagatcg taaaagacga tctcttcctc
     1081 aagttgcgta tagagatccg ggttgaacga gagggccatc tccctgagga tatcctcccg
     1141 catccgggtg agcgtcacgt acacgatcct ccccgggagg aggaggtgtt cgcccgcctt
     1201 cccgctccgc ttcagggtcg caaggtgcat gatcgatgtc tgcacaaact cgacgttgcc
     1261 ggcaccgtgc tccccgagga gcagcaccac cgatcccggc ggggcgcccc cgtccagcaa
     1321 ggggtcgagc gatgtgatgc ccgtcggtac ccgtaatcgg aggcgttcgt gcatggatac
     1381 atcctgtata taaggagaac gatttatgta ttctgatctc gcacaggatc ggtggaagtt
     1441 gcggcagaag gcagcagaaa acagatttat atggatgccg ctccagatca cctagtgttt
     1501 atgcggattc ccggcgggag gtgcctatcc cccggggacc tggacctaca acgatgcgga
     1561 tcaaggagat tatgcaacag acagcaggat atcttctgac gaagacacgt acgggtgcgc
     1621 cgttggcgcg gatgccgcga tgacgcaggg catcccccat ccggcggcaa aagagcctcc
     1681 ctcccgcgag aggggcaccc tctcccgcct cctggaccat tcctgcttct tccggaaggt
     1741 cggtccggcc gcaggcaggt acgacttcgc ggagcacggc ccccttgtgg aggcagaacc
     1801 cccttccggc tacgaagagc tcgaccgcta ctggatcgcc gcaggcctct cccttgccgt
     1861 gatcgctaaa aacacccgga cgaaccaggc cgaatacctc ctcttcgagc cggtgctctc
     1921 ggagttcgag tatgaactcc tcgaacggct ctttgacgac ctccgcgacg tcctgatcct
     1981 tgacgatcac gacctcattg ccgatcgccg ggtggtcctc tctcggaagg cgcaggacct
     2041 gttcgcggag tacggcctca cgctcgacga cacctcggcc ttcaagatcc gctactacct
     2101 tgagcgcaac ttcctcggct ggtcgcgcat cgatgcgctg atgaaagacc ctcgtatcga
     2161 ggatatatcc tgtgacggca tccgaatacc gctcttcctc taccatcgcc ggcaccagaa
     2221 catcaagacg aacatcttct ttgacgaacc ggcattaaac tcgctcgcga tcacccttgc
     2281 gcaacgttca ggcaagcacg tctcgatagg gagcccgctt gtggatgcaa ccctccccga
     2341 cggttcacgg ctgcagctca ccttcggcag cgaggtcacc acccggggta catcgttcac
     2401 gatccggaag ttccgcgaga cgcccttcac gcccgtcgaa ctgatggaga caaagacctt
     2461 cgacgtcgac cagctcgtct acttctggat ggcgatcgag aacaacaaaa gtctgctctt
     2521 cgtcggcggc accgcgtcgg gaaagaccac atcgctcaac gcggtcgccc tcttcatacc
     2581 gccgcttgca aagatcgtct ccatcgagga tacccgcgag atcacgctct accacgagaa
     2641 ctgggttgcg accgtcacgc gcgagaggat atcggaaggg ctcggcgcca ccatcgggat
     2701 gtttgacctc ctcaaggccg ctatgcggca gcgccccgag tacatactgg tcggcgaggt
     2761 caggggtaac gaggcccaga ctctctttca ggcaatgaac accgggcata cgacgttctc
     2821 caccatgcac gccgggggcg tcgacgcggc gatccaccgc ctggagaacg caccgctcaa
     2881 cgtcccacgg aacatgctcc aggcgctcga catcatatcc gtccaggcgc tcacccaccg
     2941 gggatacgac cgcgcgcgga ggtgccggga gatcgtcgag atcgccggga tcgatcccat
     3001 caccggcagc ctccaggtga acacggtcta cgagtacaac cccgtgcccg ataccttctc
     3061 ctactcgggg cgatcacgga tcctcggcga gatcatggag taccgcggct ggagccggac
     3121 ccgtctcgac gaggagatgc agatccggcg ttccgtcctt cttgcgatgc agaatcaggg
     3181 catcaaagac tacaggtcgg tcgcccggat catccaggcc tttacgatcg agcctgaccg
     3241 ggtgctcgct tctcttgacg acctcggcag gttgatccgg tgatcctgcc tccctgcata
     3301 cggatccgcc gtcttatccg ccggttggtg gagcgcgacc ccatacgcta ccggagcctc
     3361 cgcgaagacc ttgttgcggc gaaccttggt gtcaccctcg accggtacct cctcctgacg
     3421 ttcctcgcct ccgggctctt cggcgccttc tgggctatcc tggtcttcct cgtgctacga
     3481 tttgcagttc tcccgcggat gagcatcggg atctacaacg tctttgccat ccacctcccg
     3541 gaggtcatgc tggtggatcc catgatcagc attctccagg tcgtcgcgag cctggttgtc
     3601 ttcctcatca ccgcctatgc agcctctctc gtcttcctgc agtatccaac cctcgtaaag
     3661 aagaaccggg cgacccggat caacctgctg ctccaccacg ccgtcgccta catgtatgcc
     3721 atgcgccagg gcggggcgga gatgatgggg gtcttccggg cgatcggcgg gaactctgca
     3781 gtctacggcg aagcggccca cgaattccag cgtgttatcc gggataccga ttacttcggc
     3841 tacgaccaga tcgcggctct ccgacacctg caggagacga ccccgtcgga gaaactcgcc
     3901 gagttcgtcc aggatctcgt ctccgtggtc gagagcgggg gggatatgct cgccttcctc
     3961 gatgcccggg tccgcatcta ccaggaagag gcccggttcg agcagaagac cttcctctcc
     4021 acgctccagc tcgtagccga ggcatacgtg acgctcttcg ttgccggccc gctctttatc
     4081 atcatcgtca tggtggtcat ggggtttatg ggcagcacgc ccatcctgca gctctcggtt
     4141 atcatcta
//
LOCUS       DAOSII010000154         4148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_18965_length_4148_cov_38.121183, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000154 DAOSII010000000
VERSION     DAOSII010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18965_length_4148_cov_38.121183"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1680)
                     /gene="glgP"
                     /locus_tag="PLV90_09265"
     CDS             complement(<1..1680)
                     /gene="glgP"
                     /locus_tag="PLV90_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOF97341.1"
                     /translation="MAVRDPICGMPVDPAKAAYSVEVRGVTYYFSSELCRDTFVDGEK
                     IAYFSMEIGLESDIPTYSGGLGVLAGDVIRSSADLLIPMVAVTLVNRKGYVRQRLTPE
                     GDQIDLPDEWDPKKHMRELSRRVTVRIGDANVTIRAWLYDYWSPVGGLVPVLFLDTDL
                     PENAPEDRGITDHLYGGDQECRLKKAIVLGIGGARMLTALDIKVGKYHINESHSSLLT
                     LELLRRPGMDEEKVQTHCIFTTHTPVAVAFEKFPRDMVVHLLEGEIPPELLDRYIARG
                     GLALATLAFRLSRYINGVTTAHMNFSRQLFPGFHIRAVTNGVHPHTWTSVPFRNLYDR
                     YISGWAQEPELLARVDTIPREEIRHAHQQAKQALVNYVKEKNNVDLDPRALTIGFARR
                     AAAYKRATLLFSDLDRLREASRKGAIQLVFSGKAHPTDVAGKKEIRAIHDAMHDLEGE
                     VTGVYLENYTMDIAGLMTAGVDVWLNTPLPPYEASGTSGMKAALNGVVNFSILDGWWI
                     EGWIEGTTGWAIGPGPGAKVSEAERLREALQDLYSKLEYLIVPTYYRWEDEW"
     gene            2173..>4148
                     /locus_tag="PLV90_09270"
     CDS             2173..>4148
                     /locus_tag="PLV90_09270"
                     /EC_number="7.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasma-membrane proton-efflux P-type ATPase"
                     /protein_id="HOF97342.1"
                     /translation="MVESQTISTDKARSMAVDDLYQALSSGQGGLGQSEAESRIRKFG
                     PNEIVEKEESEILKFFRYFWGPIPWMIEAAVIISAIIRHWEAFAVILALLLINAIVGF
                     WQEHQAGNAIAMLKKRLALEARALRDGKWQKIAARDLVPGDIVRVRNGDIIPADIKLV
                     EGDLLSVDESALTGESMPVEKHTSDIAYSGSAVKQGEMTALVVATGEKTFFGKTARLT
                     EEAMTASHFQKAVIRIGDYLIVLAIVLVTIVFIVSLFRQESVLDTLQFALVLIVAAIP
                     AALPAVLSITMAVGATALARKEAIVSKLVAIEEMAGVDILCSDKTGTITENKLTLAEV
                     APFEGFNDDDVLLAAILASREEDQDPIDIAIIGSKEAQDLKDRLKAYTVTKFKPFDAV
                     VKRTEATVHDSDGREFSVAKGAPQVILALAGGGKDLDEAIDRLSETFAKKGYRVLGVA
                     RSDTPGTWVYVGVLGLHDPPRADSAATIKTAEEMGLDVKMVTGDHVAIAREVAREVNL
                     KTNIVTAEAFLGKHDDEAAEAVEKADGFAEVFPEHKYRIVSLLQSRGHTVGMTGDGVN
                     DAPALKKANVGIAVAGATDAAKSAAAIVLTKPGLSVIIDAIKESRRIFQRMDHYVIYR
                     ITETINVLFFIALAILIFGFFPVTALMIVLLALL"
BASE COUNT          787 a         1324 c         1275 g          762 t
ORIGIN      
        1 ccactcatcc tcccagcgat agtaggtggg gacgatcagg tactcgagtt tgctgtagag
       61 atcctggagt gcctccctca gccgctcggc ctcgctcacc tttgcgccag gtccggggcc
      121 gatcgcccat cctgtcgttc cctcgatcca gccctcgatc caccacccgt cgaggatgct
      181 gaagttgacc acgccgttca gagcagcctt catgccgctc gtgccgctcg cctcgtaggg
      241 cgggaggggc gtgttcagcc agacgtccac cccggcggtc atcaggcccg caatatccat
      301 cgtgtagttc tcgaggtaga cgcccgtcac ctccccctca aggtcgtgca tggcgtcgtg
      361 aatcgcgcgg atctcctttt tgccggcaac atccgttggg tgtgctttgc ctgaaaagac
      421 gagctggatc gctcccttcc tgcttgcctc ccgcagtctg tcaaggtccg agaagagcag
      481 ggttgcccgc ttgtaggctg ctgctcgccg ggcaaacccg atcgtgagag cccgggggtc
      541 caggtccaca ttgttcttct ccttgacata gttcaccagg gcctgctttg cctgctggtg
      601 ggcgtgcctg atctcctcgc gcgggatcgt atcgaccctg gcgagcaatt ccggttcctg
      661 cgcccacccg gagatgtagc ggtcatagag attccggaac gggaccgatg tccacgtatg
      721 agggtggacg ccgttcgtca ccgcccggat gtgaaacccg gggaagagct gtcgcgagaa
      781 gttcatgtgc gccgtcgtca cgccgttgat gtacctgctc aggcgaaacg caagggtcgc
      841 gagtgcaagc ccgcccctgg cgatgtacct gtcgagcagt tccggcggga tctcgccctc
      901 cagcaggtgg accaccatat cgcgcggaaa cttctcgaac gccaccgcca ccggggtatg
      961 ggtggtgaag atacagtgcg tctgcacctt ctcctcatcc atccccggac gcctgaggag
     1021 ttcgagggtg aggagacttg agtggctctc gttgatgtga tacttgccga ccttgatgtc
     1081 cagagcggtg agcatccggg ctccgccgat accgagcaca atcgcctttt tgagccggca
     1141 ttcctggtcg ccgccgtaga ggtgatcggt tatcccccgg tcttcgggcg cgttctcagg
     1201 gagatcggta tcgaggaaga gcaccggcac gaggccgccg acagggctcc agtagtcgta
     1261 gagccaggcc cggatcgtga cgttcgcatc cccgatccgc accgtcaccc gccgggagag
     1321 ctcccgcatg tgcttctttg gatcccactc gtcaggcagg tctatctggt cgccctccgg
     1381 ggtcaggcgt tggcgcacgt agcctttccg gttgacgagc gtcaccgcga ccatcggtat
     1441 gaggaggtcg gcgctcgaac ggatgacgtc cccggcaagt acgccaagac cgccactgta
     1501 ggtcggtata tcgctctcaa ggccgatctc catggagaag taggcgatct tctcgccatc
     1561 gacgaacgta tccctgcaga gctcgcttga gaagtagtag gtcacgccac ggacttccac
     1621 cgagtaggct gcctttgccg ggtccaccgg catcccgcag atggggtccc tcaccgccat
     1681 ggccctgcat cccccggaca ccatatgaac ctgccgatac cccggggctc gggccgggtt
     1741 atgcggcaca gcaccatctg catacacctg cgacccgatt gccgccggat atacgcgcgg
     1801 acgactcatc cccgggtgtt cgagcgccat tcccgatcat cgcaggagag gagcggggga
     1861 ggccggcaga gaggcgcggg cggggtaagg gtgctggaga acgatgcacg ggtatctccc
     1921 gtcgaaggca tgggccgggg gaggccggcg gggtgtatgg gcatctccgc agaaaggccg
     1981 gcagggcggg gaaccggaga ggaggaggga tatctgctgg aggatgtgga ggaggatcct
     2041 ctacaggggg aaaacccacc ggaaaggctg ttctcatcta tccgtccctc cgccggcttt
     2101 ctcgcaccgg gccccggggc cggcagcggc acttatatac gatcccggtg agagggggcg
     2161 ggcgggaaga ccatggtgga gagccagacg atcagcaccg ataaagccag aagtatggcc
     2221 gtcgacgacc tctaccaggc cctctcctcc gggcagggag gtctcggcca atccgaggcg
     2281 gagagccgca tccggaagtt cggccccaac gagatcgtcg agaaagagga gagcgagatc
     2341 ctgaagttct tccgctactt ctggggcccc atcccctgga tgatcgaggc ggcggtcatc
     2401 atatccgcca tcatcaggca ctgggaggcc ttcgcggtca tactcgccct ccttctcatc
     2461 aacgcaatcg tcggtttctg gcaggagcat caggcgggca acgccatcgc catgttaaag
     2521 aagaggctcg cactcgaggc ccgggcgctt cgcgacggca agtggcagaa gatcgccgca
     2581 cgagacctcg ttcccgggga catcgtccgg gtcaggaacg gagacatcat acccgcagat
     2641 atcaagctcg tcgaaggaga cctcctctcg gtcgatgagt cggccctcac cggcgagtcg
     2701 atgccggtcg agaagcatac atcggatatc gcctactccg gttccgccgt caagcagggt
     2761 gagatgactg cgctcgtggt ggccacggga gagaagacct tctttggcaa gaccgcccgg
     2821 ctcaccgagg aggcgatgac ggcgagccac tttcagaagg ccgtcatcag gatcggcgac
     2881 tacctgatcg tgctcgcgat cgtcctcgtc accatcgtct tcatcgtctc gctctttcgg
     2941 caggagagcg tccttgatac cctccagttc gcactcgtcc tgatcgttgc ggcgattccc
     3001 gccgcgctgc cggccgtcct ctcgatcacc atggccgtgg gggcgacggc acttgccaga
     3061 aaagaggcca tcgtcagcaa actcgtcgca atcgaggaga tggccggtgt ggatatcctc
     3121 tgctcggaca agaccggaac gatcaccgag aataaactta ccctcgccga ggtcgccccc
     3181 ttcgaggggt tcaacgatga cgatgtactc cttgccgcca tcctcgcatc tcgcgaggag
     3241 gaccaggacc ccatcgatat cgctattatc ggctcaaaag aagcgcagga tctcaaagat
     3301 cggctcaagg catataccgt caccaaattc aagccctttg atgcggtagt gaaacgcacc
     3361 gaggccaccg tccacgatag cgacggccgg gagttctcgg tcgcaaaagg tgcaccgcag
     3421 gtgatcctcg ccctcgccgg cgggggaaag gatctcgatg aggcgatcga ccgtctctcc
     3481 gagaccttcg caaagaaagg ctaccgggtt ctcggcgttg cacggagcga cacccccggt
     3541 acctgggtat acgtcggcgt cctcggtctc cacgacccgc cccgcgccga ttccgcggca
     3601 accatcaaga cggcggagga gatggggctc gacgtcaaga tggtcaccgg cgaccacgtg
     3661 gcgatcgccc gcgaggtcgc ccgcgaggtg aacctcaaga ccaacatcgt caccgccgaa
     3721 gccttcctcg ggaagcacga cgacgaggcc gcagaggccg tcgagaaggc agacgggttt
     3781 gccgaggtct tccccgagca taaataccgg atcgtctccc tcctccagtc ccggggacac
     3841 accgtcggca tgaccggcga cggcgtgaac gatgcccccg ccctgaagaa ggccaatgtc
     3901 gggatagcgg tcgccggggc cacggatgcg gcgaagtccg cggcggcgat cgttctcacg
     3961 aaacccgggc tctcggtgat catcgatgcg ataaaagaga gccggaggat ctttcagcgc
     4021 atggaccact atgtcatcta ccggatcacc gagaccatca acgtcctctt cttcatcgcc
     4081 ctcgcgatcc tgatattcgg gttctttccg gtcaccgccc tgatgatcgt cctcctcgcc
     4141 ctcttaaa
//
LOCUS       DAOSII010000155         4141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_19011_length_4141_cov_38.772149, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000155 DAOSII010000000
VERSION     DAOSII010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19011_length_4141_cov_38.772149"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(238..684)
                     /locus_tag="PLV90_09275"
     CDS             complement(238..684)
                     /locus_tag="PLV90_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3887 domain-containing protein"
                     /protein_id="HOF97343.1"
                     /translation="MNLPRLSLFVVLAVLLGISTAGCMDRETVLSGDDATEVLAYAEP
                     IVDNLMQGFNEGNYAKYSRDFGPEMKQALDEAAFAENHEFVTSRIGLHESRGDPVVTK
                     TGEYVAVTYRAKFEREDGVALQFVFEKDDPSHRLQGLWFDSPMLRS"
     gene            complement(728..>2605)
                     /locus_tag="PLV90_09280"
     CDS             complement(728..>2605)
                     /locus_tag="PLV90_09280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF97344.1"
                     /translation="ASNEEELRRSFDDLAESQRQLQQSEDLYRRISGSISDIVYACDL
                     QDTGGYALSLIAGAVSRITGYTAEEVLAMRCWKALVHPDDHSVFERDIVPLSPGMSSV
                     TELRIIHRDGSVRWIRVTANCTTGQDGCPRMYGGWQDVTAQKKAEEALRESEQHFRTL
                     ADSGQALIWACGLDAGWDYFNEPWLAFTGRTLEEEIGDGWMEGVHPDDRSRCIEIFTG
                     AFARRKPFSMTHRLRRYDGEYRWVQDDGSPRYDTRDNFLGFIGHCLDVTELRRMEEML
                     QETLEMMEGIFLVAPTGIGMTVGGVFTEANLQLCEITGYTRDELIGKPMRLLYPGETE
                     YETALEVMRAQIKMTGRCTIETRFLRKNGTSSDVQLAIALFDPADLSRGMALTVLDIT
                     ESKAMEREIESHARELLRQTSSLAAVNKKLNLMNSITRHDVLNQLTILLGNLSFAQEA
                     RPGQDISRFLSPVKDAAGRIQRQIEFTRDYADLGIGEPEWQRISDAIRSAAYEFPTED
                     ESGDLAIYADPMLARVFANLMDNTIRHGGQVSRVRVRYWLEGDGALTLAWEDDGIGVP
                     AKEKEEIFKQGIGKNTGLGLFLIREILGITGIAITETGEPGKGARFEMRVPEGMYRFG
                     E"
     assembly_gap    2606..2705
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2706..4083)
                     /locus_tag="PLV90_09285"
     CDS             complement(<2706..4083)
                     /locus_tag="PLV90_09285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF97345.1"
                     /translation="MAASTPVQALYVDDEAVLLDIARLYLERTGDIAVDITANPLEAY
                     TLIMTGRYDVVVSDYQMPEMDGIALLKRAREAGSQVPFIIFTGKGREEVVIEALNNGA
                     DFYLQKGGDPKTQFAELANAIRQLAGKQKAEIAVRRSEEMLHKAESLAHLGSWVFDHR
                     TGRLTWSDETYRIFGLDPGERVMTHESFLSMVHPDDRAMVHATYSSSVTKGEASYTVE
                     HRIFRADTGEVRYVAERCEHLRDALGEATLSVGMTHDITERKLAELELLQRHEELRAA
                     YEQLASIEEELRTSFDDLAGSQRRIQESEAKLADIIDFLPDATFVIDTDGCVIAWNRA
                     MEKMTGIPKAEMLGRGDYAYAVPFYGEACPVLIDYVLHPGREADSPYQTEKISEDILD
                     GETSRARLAGRAVALKIRASPLYDREGRCVGAIEIVRDITGQKQVEADLRETKKYLEN
                     LIDHASAAI"
BASE COUNT          720 a         1277 c         1221 g          823 t
ORIGIN      
        1 ggtagcgcac cgggcgataa ccctcgcagg gggccgcgat cgtcgtccgg ggcgagggcg
       61 acgaccccca cacgcccgac aggaccgact gcgatatcca cggcagcccg gtatgccagg
      121 cgttgcggaa taggatcagg cccggcccgt ggtcgatgct cgccggccgg atgaagggtg
      181 agcgggaccc cgctctgatc cggcgacgcg gctgcagggg gaggccggcg gtgcgcctca
      241 actgcgcagc atcggggagt caaaccagag gccctggagc cggtgcgacg gatcgtcctt
      301 ttcaaagaca aattggaggg ccacgccgtc ttcccgctcg aatttcgcac ggtaggtcac
      361 ggcgacgtac tcgccggtct ttgtgacgac ggggtcgccc cgggactcat gaagcccgat
      421 acgggacgtg acgaactcgt ggttctctgc aaacgcggcc tcgtccaggg cctgcttcat
      481 ctcggggccg aaatcgcggg agtacttcgc atagttgcct tcgttgaaac cctgcatgag
      541 gttatcgacg atcggttcgg catatgcgag cacttctgtc gcatcatcgc cggagagcac
      601 ggtctcccgg tccatgcagc cggctgtaga gatacccaga aggaccgcga gaacgacgaa
      661 gagtgagagt cgggggaggt tcatgcggta ctatcgaccc gggaggatat gaatggagga
      721 gatggcctca ctccccaaac cggtacatcc cctcgggcac gcgcatctca aaccgtgccc
      781 ccttcccggg ctctcccgtc tcggtgatgg cgataccggt gataccgagg atctcccgga
      841 tcaggaagag gccaagtccg gtgttctttc cgataccctg cttgaagatc tcctccttct
      901 cttttgcagg aacgccgatg ccgtcgtcct cccaggccag ggtgagagct ccgtccccct
      961 ccagccagta ccggacacgg acccggctca cctgcccccc gtgacggatc gtgttgtcca
     1021 tcaggttggc aaagaccctt gcaagcatcg gatccgcgta gatggcaagg tctccggact
     1081 catcttccgt agggaactcg tacgccgccg atcggatggc atcggagata cgctgccact
     1141 caggttctcc gataccgaga tccgcatagt cccgggtgaa ctcgatctgg cgttgtatcc
     1201 gcccggcggc gtccttcacc ggggagagga acctcgatat atcctggccg ggtcgtgcct
     1261 cctgtgcaaa cgagaggttg ccaaggagga tcgtcagctg gttcaagacg tcgtgccggg
     1321 tgatgctgtt catcaggttg agttttttgt tcacggcggc cagactgctc gtctgacgga
     1381 gaagctcccg ggcatgagat tcgatctccc gctccatcgc cttgctctcg gtgatatcca
     1441 gtacggtgag cgccatgccc cgggagaggt ccgccgggtc aaacagcgcg atagcaagct
     1501 ggacatcgct gctcgtgccg ttctttcgca gaaaacgggt ctcaatcgtg catctcccgg
     1561 tcatcttgat ctgcgcacgc atcacctcaa gggcggtctc gtactcggtc tccccgggat
     1621 agagcagccg catgggcttg ccgatgagtt cgtcgcgcgt ataaccggtg atctcgcaga
     1681 gttgcaggtt cgcctccgta aacaccccgc ccactgtcat accgatgcct gttggggcga
     1741 cgaggaagat gccctccatc atctccaggg tctcctgcag catctcctcc atcctccgga
     1801 gctcggtgac gtccaggcag tgcccgatga aaccgaggaa gttgtcccgg gtgtcgtacc
     1861 ggggggagcc gtcgtcctga acccagcggt actctccgtc gtaccgtcgc aggcggtggg
     1921 tcatgctgaa cggtttacgc cgggcaaagg cgcccgtgaa gatctcgata catcgcgagc
     1981 gatcgtcggg gtgcaccccc tccatccagc cgtcaccgat ctcctcttca agcgtccggc
     2041 cggtgaaggc aagccatggt tcgttgaagt aatcccaccc tgcatccagc ccgcacgccc
     2101 agatcagggc ctggccgctg tccgcaaggg tgcggaagtg ctgttcgctc tcccgcagcg
     2161 cctcttcggc ctttttctga gcggtaacgt cctgccaccc gccgtacatc cgcgggcaac
     2221 catcctgacc ggtggtacag ttggcggtga cgcggatcca ccggaccgaa ccgtcccggt
     2281 ggattatccg gagctcggtg accgacgaca tacccgggga gagcgggacg atatcgcgct
     2341 cgaatacgga atggtcgtcg gggtggacaa gagccttcca gcatcgcatc gcaagaactt
     2401 cttctgcggt atacccggtg atcctggata ccgctccggc aatcaggctg agggcatacc
     2461 cgcccgtatc ctgcaggtcg cacgcgtaga caatgtcgga tatgctcccg gatatccgcc
     2521 ggtagaggtc ctcgctctgc tggagctggc gctggctctc ggcaaggtcg tcgaagcttc
     2581 tccggagttc ctcctcgttg gaggcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnncgatc gcggcgctgg cgtgatcgat caggttctcc agatactttt tggtctcgcg
     2761 caggtcggcc tcgacctgtt tctgcccggt gatatcccgg actatctcga ttgcgccgac
     2821 acacctgccc tcccggtcat agagagggga tgccctgatc ttgagggcga ctgcccttcc
     2881 ggcaagacgg gcacgagaag tctcgccatc gaggatatct tcgcttatct tctcggtctg
     2941 gtacgggctg tctgcttctc tcccgggatg gaggacataa tcgatcagga ccgggcatgc
     3001 ctcgccgtag aacgggaccg catatgcata atcgcccctc ccgagcatct ctgccttcgg
     3061 tatgccggtc attttttcca tcgcgcggtt ccaggcgatg acgcagccgt ccgtatcgat
     3121 gacgaaggtt gcatcgggga gaaaatcgat gatatcggca agcttcgcct cgctctcctg
     3181 gatccggcgc tggctcccgg caaggtcgtc gaagcttgtc cggagctcct cctcgatgga
     3241 ggcaagctgc tcgtacgccg cccgcagttc ctcgtgcctc tgcagcagtt cgagttcggc
     3301 gagtttacgc tcggtgatat cgtgcgtcat cccgaccgag agggtggcct cccccagtgc
     3361 gtcccgcagg tgttcgcacc gttcagcgac ataccggacc tccccggtgt cggcacgaaa
     3421 tatccggtgt tcgacggtat aggaggcctc tcctttcgtg acagaggaag agtaggttgc
     3481 atggaccatg gccctgtcgt ccgggtgcac catcgagagg aacgactcgt gggtcatcac
     3541 acgctccccg ggatcgaggc cgaatatgcg gtaggtctcg tcagaccacg tcaaccgccc
     3601 ggtcctgtga tcgaacaccc agctcccgag atgcgcaagg ctctcggcct tatgcagcat
     3661 ctcctcgctc cggcgaaccg cgatctccgc cttctgcttc ccggccagct gccggatagc
     3721 gtttgcaagc tcggcaaact gcgtcttcgg atcgcccccc ttctggaggt agaagtcggc
     3781 gccgttgttc agcgcctcga tcaccacctc ttcgcgtccc ttgccggtga atatgatgaa
     3841 gggcacctgc gatccggcct cccgggcccg cttcaggaga gcgataccgt ccatctccgg
     3901 catctggtag tccgagacga ccacgtcata ccggccggtc atgatcagcg tatacgcttc
     3961 aagcgggttt gccgtgatat cgacggctat atcgcccgtt cgctcaaggt agaggcgggc
     4021 gatatcgagc agcacggcct cgtcgtcgac atagagtgcc tgaacggggg ttgatgcggc
     4081 catatacggt tgatacctac gtgtagatgt agtagggcaa tccttaaacc tttcgaattt
     4141 g
//
LOCUS       DAOSII010000156         4133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_19049_length_4133_cov_57.724375, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000156 DAOSII010000000
VERSION     DAOSII010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19049_length_4133_cov_57.724375"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLV90_09290"
     CDS             complement(<1..233)
                     /locus_tag="PLV90_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOF97346.1"
                     /translation="MKRDAVAYLNTRRKGDFAAQKDEVENYCKYRFQIAAIFHDHRAG
                     ATHPERREGFLAMLEYCAANDCPEIIIYDLADLA"
     gene            complement(342..1019)
                     /gene="ribB"
                     /locus_tag="PLV90_09295"
     CDS             complement(342..1019)
                     /gene="ribB"
                     /locus_tag="PLV90_09295"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HOF97347.1"
                     /translation="MIDAAIQALARGEFVLLYDFDNRERETDFAIRSDAVTPAHIRQM
                     RKDGGGLICTAVHPDAAKRLGLPFAHDLLRACSLVEKDGEVPYDRKNHSSFSLWVNHR
                     ETYTGITDRDRALTVTAIAEEVKRSLNGGGHDFAAHFRTPGHMALLRAADRLLDERRG
                     QTELSIALAAMADVTPAVTICEMLDDDTGFALSKEGAMEYARKHGLVFVEGQDVLDRW
                     ESQKQGR"
     gene            complement(1012..1680)
                     /locus_tag="PLV90_09300"
     CDS             complement(1012..1680)
                     /locus_tag="PLV90_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF120 domain-containing protein"
                     /protein_id="HOF97348.1"
                     /translation="MVEAEDLQSLKMIALLGGCQGPIWLSSQSLASALGISPQTASRR
                     LIALERQQFVVRSMKPDGQYVAITRAGEQTLRREYADYVRIFDPERRQYVLTGTVISG
                     LGEGRYYMSLPHYKEQFSGSLGFEPFPGTLNIRLDAASVQVRKHLDHAGWIDIPGFTA
                     DERTFGGARVLPCRIRGEVCAIVEPSRTHYPEDIIEVIAGHELRKTLNLNDNDTIEVE
                     ITHD"
     gene            1803..2513
                     /locus_tag="PLV90_09305"
     CDS             1803..2513
                     /locus_tag="PLV90_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOF97349.1"
                     /translation="MKSGVRHQLAEKMAGEITLSDSPGQALKKWRQSFNIPQGLLAER
                     LEVSPSVISDYESGRRKSPGTAIVGKIVDAILSIDEDSGGRFINKFAKILYIEFDEDV
                     IYDIHEYASPVSLSDFAGAIDAATLCGPMDQTIYGYTVINSLNAILQLSSSEFNRIYG
                     WSTERALIFTEVSTGKSPMVAIRVTPFKPRCVVLQGINPEEVHPLIPGLAEHDRITVL
                     CTTMDVEAIISALRGKLW"
     gene            2507..3559
                     /locus_tag="PLV90_09310"
     CDS             2507..3559
                     /locus_tag="PLV90_09310"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HOF97350.1"
                     /translation="MVGIYSYGVYIPRYRIKVPEIARVWGANADDITRGLGVFEKSVP
                     DLDEDTATIAVEAARAALRRRKIDTGAIGAVYVGSESHPYAVKPTACTVGEAIGATPN
                     MTAADYEFACKAGTAGLQTCMGLVRSKMIPFGIAIGADVAQGAPGDALEYTAAAGGAA
                     FVVGEGETIADINRTCSFTTDTPDFWRREGQSYPRHGGRFTGDPGYFKHVQGAARLMF
                     DAMGTGPKDYDYAVFHQPNAKFPQRVAKMLGFTDAQIRPGLVVPKLGNTYSGSSMIGL
                     AATLDVAKPGERIFVTSFGSGAGSDAFDITVTDVIESEAFDRAAAPAVEKLLANPIYL
                     DYALYAKHKGKIVMQK"
     gene            3556..>4133
                     /locus_tag="PLV90_09315"
     CDS             3556..>4133
                     /locus_tag="PLV90_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOF97351.1"
                     /translation="MRDVAVIGVGCTEFGEHWSTSFRDLFVTAGALALEDAGISGEKI
                     DALYVGNMSAGRFVEQEHIGALIADYAGLASNHVPSTRVEAACASGGLAFRQAVIAVA
                     SGMEDVVVAAGVEKMTDVGTGASVDMLASAADREWEGFAGATFPGLYAMIANDYMHRY
                     PLTREQLAAVAVKNHENGAKNPIAQFRNRITI"
BASE COUNT          817 a         1267 c         1289 g          760 t
ORIGIN      
        1 gcgagatcgg ccagatcgta tatgatgatc tcggggcagt cgtttgcggc gcagtactcg
       61 agcatcgcca gaaacccctc ccgcctctca ggatgcgttg cgccggcacg gtgatcgtga
      121 aagatcgcgg cgatctggaa ccgatatttg cagtaattct cgacttcatc cttttgtgct
      181 gcgaaatctc ctttccttct cgtattcagg tatgccacag catctctctt catccgttta
      241 tggtgccgca ggaatgcata tatgatttat gaaaagtgcg ccgctaccgc cgcatccctc
      301 cgcccgcaaa acggcgagca acccctgagg agaaagaagg ttcaccttcc ctgcttctgc
      361 gactcccacc ggtcgaggac atcctgcccc tcgacaaaga cgaggccgtg cttcctcgca
      421 tactccatcg ctccctcctt agagagcgca aaccccgtat cgtcgtccag catctcgcag
      481 atcgtgaccg caggagtgac atcggccatt gcggcgagcg cgatcgagag ctcggtctgt
      541 ccgcgccgct cgtcgagcag ccggtcggcg gcccgcagaa gcgccatgtg accgggtgtc
      601 cggaagtgcg cggcaaaatc gtgccccccg ccgttgagcg accgcttcac ctcttcggcg
      661 atggcggtga ccgtcagggc gcggtcgcgg tccgtgatcc cggtataggt ctcccggtgg
      721 tttacccaga gggagaacga cgagtggttc ttccggtcgt acgggacctc gccgtccttc
      781 tcgaccaggc tgcaggcccg gaggagatcg tgggcgaacg ggaggccgag cctctttgcg
      841 gcatcgggat gcaccgccgt gcagatcagg ccgccgccgt ctttacgcat ctgccggatg
      901 tgggcgggcg tgacggcatc ggagcgtatc gcaaagtcgg tctcgcgctc ccgattgtca
      961 aagtcgtaga gcaggacaaa ttcgccccgt gcaagggcct gtatggctgc atcaatcatg
     1021 ggtaatctcc acctcgatcg tatcgttgtc gttcaggttc agggtcttgc ggagctcgtg
     1081 gccggcgatg acctcgatga tatcctccgg gtaatgggtg cgggagggct cgacgatcgc
     1141 gcacacctcg ccccggatgc ggcaggggag cacccgggcg ccgccgaacg tccgttcatc
     1201 ggcggtaaac ccgggtatat cgatccatcc tgcatggtcg agatgcttcc tgacctggac
     1261 gctcgccgca tcgagcctta tgttcagggt gccgggaaag ggctcaaacc cgagagaccc
     1321 gctgaactgc tctttgtaat gggggaggct catgtagtag cgcccttccc cgaggccgct
     1381 gataacggtc ccggtcagga catactgccg gcgctccggg tcgaagatcc gcacgtagtc
     1441 cgcatactcc cgcctcagcg tctgctcgcc cgcacgggtg atagcgacat actgcccgtc
     1501 gggcttcatc gaccggacga cgaactgctg ccgctcaagc gcgataaggc gccgcgatgc
     1561 ggtctgggga ctgatgccga gagcgctcgc gagtgactgc gacgagagcc atatcgggcc
     1621 ctggcacccg ccgagcaggg cgatcatctt cagggactgc agatcttccg cttcaaccat
     1681 tatatcataa ttgagatgca tactatatac gggttttgca acgacaccat gccggcaggc
     1741 cttcgtgaaa tgtcgatatg ggatacgcta attatataag ttccggtgct aacattagct
     1801 gcatgaaatc cggagtgcgg catcaacttg ccgagaaaat ggcgggagag atcacactct
     1861 cggactcacc gggacaggcc ttgaagaagt ggcggcagag tttcaacatc cctcaggggt
     1921 tgcttgccga gcgactcgag gtctctccct cagttatcag cgattacgaa agtggacggc
     1981 gaaagagtcc gggaaccgcc atcgtcggca agatcgtcga cgcgatcctc tcgatcgacg
     2041 aagacagcgg cggtcggttc atcaacaaat ttgcaaaaat tctgtacatc gaatttgacg
     2101 aagacgtcat ctacgacatc cacgagtacg catccccggt aagtctctcc gactttgccg
     2161 gagccatcga tgccgccacg ctctgcggcc ccatggacca gaccatctac gggtacacgg
     2221 tgatcaacag cctcaacgcg atcctccagc tctcgtcaag cgagttcaac cgtatatacg
     2281 gctggagcac ggagcgtgcg ctcatattca ccgaggtctc gaccgggaag tcacccatgg
     2341 tggctatccg ggtcaccccc ttcaaacccc gctgtgtggt cctgcagggg atcaatcccg
     2401 aagaggtgca cccgctgata ccgggtcttg cggagcacga tcggatcacg gtgctctgta
     2461 ccacgatgga tgtcgaggcg atcatcagcg cattacgagg gaaactatgg taggtattta
     2521 ttcgtacggc gtctacatcc cgcgataccg gataaaagtc ccggagatcg cgcgggtatg
     2581 gggcgccaac gcagacgaca tcacccgggg cctcggggtc tttgagaagt ccgtccccga
     2641 tctcgacgag gataccgcaa cgatcgccgt cgaggcggcc cgtgccgccc tccggcggag
     2701 gaagatcgat accggcgcta tcggtgcggt ctacgtgggg agcgagtccc acccctacgc
     2761 ggtcaaaccc accgcctgca cggtcggcga ggcgatcggg gcgacaccga acatgaccgc
     2821 cgcagattac gagttcgcgt gcaaggcggg aaccgcaggc ctccagacct gtatggggct
     2881 tgtccggagc aagatgatcc cgttcgggat cgccatcggt gcggatgtgg cccagggcgc
     2941 tcccggcgac gctcttgagt acacggcggc ggccggcggg gccgcgttcg tcgtcggcga
     3001 aggggagacc atcgccgata tcaaccggac ctgctccttc actaccgata cgcccgattt
     3061 ctggcggcgc gaggggcaga gttacccccg gcacggcggc cggttcaccg gcgaccccgg
     3121 ctacttcaag cacgtccagg gcgccgcaag gcttatgttc gatgcgatgg gcaccggccc
     3181 gaaggactac gactacgcgg tcttccacca gccgaacgcg aagttcccgc agcgggtggc
     3241 gaagatgctc ggctttaccg acgcacagat ccggcccggc ctcgtcgttc cgaagctcgg
     3301 caacacctac tcggggtcgt cgatgatcgg gcttgcggcg acgcttgatg tcgcaaaacc
     3361 gggcgagcgg atattcgtca catcgttcgg ttccggggcg gggagcgacg ccttcgatat
     3421 cacggtcacc gacgtgatcg agtcggaggc attcgaccgg gcggcggcgc cggccgtgga
     3481 aaaactcctc gcaaacccga tctacctcga ctatgcactg tatgccaaac acaaaggaaa
     3541 gatagtgatg cagaaatgag agacgtagca gtgatcggtg tcgggtgcac ggagttcggg
     3601 gaacactgga gcacgtcgtt tcgcgacctc ttcgtgacgg caggagcgct ggcgcttgaa
     3661 gatgccggga tatcgggcga gaagatcgac gccctgtatg tcgggaacat gagcgccggc
     3721 cggttcgtcg agcaggaaca catcggggcc ctgatcgcgg attacgcagg gttggcgagc
     3781 aaccacgtcc cctcgacccg ggtggaggcg gcctgcgcat ccggcgggct tgccttccgg
     3841 caggcggtga tcgcggtcgc aagcggcatg gaagatgtcg tggtcgcagc gggcgtcgag
     3901 aagatgaccg atgtcgggac cggagcgagc gtcgatatgc tcgcgagcgc cgcggaccgc
     3961 gagtgggagg ggttcgccgg agcgaccttc cccggcctct acgcgatgat cgcaaacgac
     4021 tacatgcacc gctacccgct cacccgcgag cagcttgccg cggtggcggt gaagaaccac
     4081 gagaacggcg caaagaaccc gatcgcccag ttccggaacc ggataacgat cga
//
LOCUS       DAOSII010000157         4131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_19056_length_4131_cov_52.031649, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000157 DAOSII010000000
VERSION     DAOSII010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19056_length_4131_cov_52.031649"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(608..1627)
                     /locus_tag="PLV90_09320"
     CDS             complement(608..1627)
                     /locus_tag="PLV90_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOF97352.1"
                     /translation="METKTFKNLEAEVWTTDLCSGCGACVAVCPADALRFVPGKTDAP
                     VNIGYCKSENDGVPCGACYAACPRVDLAGQGRMLGAYREIVAARSAFPVENKQSGGAV
                     TAILANALDEGLIDAVVTVTRDPWTLKPSSAVITSSDALIHHAGSRYSWWVPLLASLK
                     EAVVTRKYRRIAVVGVPCVARATQAIRASDHDLLRPYAKAIRLVIGLFCTETFDYAKL
                     VEEKLQSEKKIEPWEIQRLDVKGKLDVYLQDGRHIPIPLADLEECVRPGCRVCTDLTA
                     VDADISAGAIGSPDGYTTLVIRNDIGRGFVDRAVWKGKLATGGNVDIAAIERLAAKKA
                     ERRKE"
     gene            complement(1633..2370)
                     /locus_tag="PLV90_09325"
     CDS             complement(1633..2370)
                     /locus_tag="PLV90_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97353.1"
                     /translation="MTKLVTIDAKGVHYTPLNQQIRKAIEDGADEIVVDNVLGQRFIG
                     NGLRGNAVIRVNGVAGGDLAMFMSGPTVVVHGNAEHAPGNTMDSGKVVIHGSAGDAVA
                     HSMRGGKVFVRGDIGYRGGIHMKQYEAHRPILVVGGSAQAFLGEYMAGGLLLVLALDG
                     AMKAREIGSGIHGGEIVIRGNVDEACLAAGAKTLPLTDEDRARIAPVIREFADDFGLD
                     PEPLVNADYTRIVPASARPFAGKYTWE"
     gene            complement(2376..3893)
                     /locus_tag="PLV90_09330"
     CDS             complement(2376..3893)
                     /locus_tag="PLV90_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOF97354.1"
                     /translation="MSSNIGSMPLRYRVTIDRERCMECGRCIENCSYGVFRWDGDRIL
                     INSRNCTACHRCLAYCPRDAIMLEEHPCDYRSHPVWSRQVRESIYNQARTGKIILAGM
                     GSVADLPIIFDHLMLDACQVTTPPTDPLREPVELRTYLGKKPSRLDFARKPNGDIEVS
                     TELAPNLMLETPIMLGHMSYGAISLNAHLAMARAAKETGTYMGTGEGGLHPGLYPYQD
                     RMIVQVASGRFGVNVDYLERGAAIELKLGQGAKPGIGGHLPGEKVSADVSRTRMIPEG
                     SDAISPAPHHDVYGIEDLPQLVNSVKEATGWKKPVFAKIAAVNNNAENVAAIARSGVD
                     AVAIDGFRGGTGAAPRVFRDHVGIPIEVAIATADRELRKQGLRNEVSLIACGSIRESA
                     DVVKAIALGADAVYIGTAALVAMGCRVCGNCYQGVCSWGIATQRPNLVARLDPDVASK
                     QVANLIHAWTLEITELMGAAGINSIESLRGNRDRLRGYMLDEGLLEILDVKSVGA"
     gene            complement(3890..>4131)
                     /locus_tag="PLV90_09335"
     CDS             complement(3890..>4131)
                     /locus_tag="PLV90_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97355.1"
                     /translation="LRFAYGPATMNGPFTVVAANPDMMVGFTDRGKLRPMVVGECGDR
                     LYIASEETAIRTMEPGVESITRPGAGEPVIGRVAP"
BASE COUNT          654 a         1380 c         1303 g          794 t
ORIGIN      
        1 gatccccgac cctgcggagc atgtcgaggg catagatgag gtctgcaacc gtatcagccg
       61 gaaatggaga tccgccgtgc ggtctgcccg ttctggttta cgcgccgata cagattccgg
      121 cacctcttta tgaacctggt gcaatcttct cgcccctcag atcgaactat tcacataagt
      181 atgatttttc ataccatatg aaccccctgt tccagtactt tgctcatccg gcacccgcct
      241 caaatgtggt ctcgcggcgc cttaacgcgg gaccggcagc acccggacac cgggcttcgc
      301 ggcttcgcgc gagacggcag ggtatacgcg atgaagcggc ctctcgcgaa ggcgcgaagg
      361 gatgttccag gcccccctct accgaagttc gcgtccttcg gggctcgcgc gagctcgagg
      421 gtatgggcga cggaacaacc ccgcgcgaag atgcgaaggg gaatctgtag tttgacttct
      481 ccgtgggatt ttccggaact tgagcgaagt gctgcggtcg tgcgcatcct ccatcatccc
      541 cgatcaagcg ggcagaggct ttgccccggg gctccggcag atcggggagc acgttgcaaa
      601 aaaagtgtta ttctttccgg cgctctgctt tcttcgccgc caggcgctcg atggcggcga
      661 tatcgacgtt cccgccggtt gcaagctttc ccttccagac agcccggtcg acgaaacccc
      721 tgccgatatc gtttcggatc acgagcgtcg tgtagccgtc cggcgacccg atggccccgg
      781 ccgatatatc cgcatccacc gccgtaaggt cggtgcagac ccggcacccg ggccggacgc
      841 actcctcgag gtcggcaagc gggatgggga tgtgccgccc gtcctgcagg tatacgtcca
      901 gtttcccctt gacgtccagg cgctggatct cccagggttc gatcttcttc tcggactgca
      961 gcttctcctc gaccagcttc gcgtagtcga aggtctccgt gcagaagaga ccgatgacga
     1021 gccggatagc cttcgcgtag ggccggagga gatcgtggtc gcttgcccgg atcgcctggg
     1081 tcgcccgcgc cacacagggc acgcctacga cggcgatccg ccggtacttc cgggtgacca
     1141 ccgcctcctt caaggaggca agcagcggca cccaccagga gtatcggctc ccggcgtgat
     1201 ggatgagcgc atccgacgac gtgatcaccg ccgaggacgg tttcagcgtc caggggtctc
     1261 tcgtcaccgt gacgaccgca tcgatcagcc cttcatcgag ggcgtttgcg aggattgccg
     1321 tcaccgcgcc cccgctctgc ttgttctcca cggggaaagc cgaccgggcc gcgacgatct
     1381 cccggtaggc cccgagcatc cgcccctgcc ccgcaaggtc gacccggggg caggccgcgt
     1441 agcaggcccc gcagggcacg ccgtcgttct cggacttgca gtagccgatg ttcaccggcg
     1501 catcggtctt ccccggcaca aaccggaggg catccgccgg acagaccgcc acgcatgctc
     1561 cgcacccgga gcagaggtcc gtagtccaga cctcggcctc caggttcttg aaagtcttcg
     1621 tctccatccc catcactccc acgtatactt cccggcaaag ggccgtgcgc tcgcagggac
     1681 gatccgtgtg taatccgcgt tcacgagcgg ttccggatcg agcccgaaat cgtccgcaaa
     1741 ctcccggatg accggggcga tccgtgccct gtcctcatcg gtgagcggca atgtcttcgc
     1801 tcccgcggcg aggcaggcct catccacgtt cccgcggatg acgatctcgc cgccgtggat
     1861 accgctcccg atctcccggg ctttcatggc cccgtcgagt gcgagaacga gcagaaggcc
     1921 acctgccatg tactcgccga ggaacgcctg cgcagacccc ccgacgacca gtatcggccg
     1981 atgggcctcg tactgcttca tgtggatccc gccccggtag ccgatatccc cgcggacgaa
     2041 gaccttgccg ccccgcatgc tgtgcgccac cgcgtcgccg gcactcccgt ggatcaccac
     2101 cttcccggag tccatcgtgt tgccgggcgc gtgttcggcg ttcccgtgaa cgacgacggt
     2161 cgggccgctc atgaacatgg cgagatcccc gcccgcgacg ccgttgaccc ggatgactgc
     2221 attcccgcga aggccgttgc cgatgaaccg ctgcccgagg acgttgtcga cgacgatctc
     2281 atccgctcca tcctcgatgg ccttcctgat ctgctggttc aggggggtgt agtgtacccc
     2341 tttcgcatca atcgtgacca attttgtcat tgtgctcacg ctcccaccga tttgacgtcg
     2401 aggatctcaa ggagcccctc gtcgagcatg tagccacgga gccggtcgcg gttgccgcgc
     2461 aggctctcga tgctgttgat gccggccgcg cccatcagct cggtgatctc aagcgtccag
     2521 gcgtggatca ggtttgccac ctgctttgat gccacgtcgg gatccaggcg tgcgacaagg
     2581 ttcggtcgct gggtggcgat gccccaggag cagaccccct ggtaacagtt tccgcagacc
     2641 cggcagccca tcgccacgag cgccgcggtc ccgatgtaga cggcatcggc gccgagagcg
     2701 atcgccttta cgacgtctgc gctctcgcgg atgctgccgc aggcgatgag cgagacttcg
     2761 ttcctgagtc cctgcttgcg gagttcgcgg tcggcggtcg cgatggcgac ctcgatcggg
     2821 atgccgacgt ggtcgcggaa gacccgcggt gccgcgccgg tgccgccccg gaacccgtcg
     2881 attgcaacgg catccacgcc ggaacgggca atggccgcta cgttctcggc gttgttgttg
     2941 acggccgcaa tctttgcaaa gaccggtttt ttccacccgg tcgcctcctt gacggagttg
     3001 acgagctggg ggaggtcctc gatgccgtag acgtcgtgat gcggcgcagg gctgatcgcg
     3061 tcgcttccct ccggtatcat ccttgtccgg gagacgtccg cgctcacctt ctcgccgggg
     3121 aggtgcccgc cgatgcccgg ttttgctccc tggccgagtt tgagctcgat cgcggcgccc
     3181 cgctccaggt agtcgacgtt cacgccgaac ctccccgagg cgacctggac gatcatccgg
     3241 tcctggtagg ggtagaggcc ggggtgcagg ccgccctcgc cggtgcccat gtaggtgccc
     3301 gtctcctttg ccgcccgggc catcgcgagg tgggcgttga ggctgattgc gccgtagctc
     3361 atgtggccga gcatgatcgg cgtctcgagc atgaggtttg gagcgagttc cgtggagacc
     3421 tcgatatcgc cgttcggttt tcgcgcaaaa tccagcctcg acggtttctt cccgaggtag
     3481 gtccggagtt cgacgggttc gcggagcggg tcggtcgggg gcgtggtgac ctggcaggcg
     3541 tccagcatca ggtggtcgaa tatgatcggg aggtcggcga cgctccccat cccggcgagg
     3601 atgatcttac ccgtccgggc ctggttgtag atagactccc tgacctgccg cgaccagacc
     3661 ggatggctcc ggtaatcgca ggggtgctcc tcgagcatga tggcgtctcg cgggcagtag
     3721 gcgaggcacc ggtgacaggc ggtgcagttc cgggagttga tgaggatgcg gtcgccgtcc
     3781 catcggaata cgccgtagga gcagttctcg atgcatcgcc cgcattccat gcaccgctcc
     3841 cggtcgatgg tgacccggta ccggagcggc atgctgccga tgttgctgct catggtgcaa
     3901 ccctcccgat gaccggctct cctgcacccg gtctcgtgat cgactcgacg ccgggctcca
     3961 tggtgcggat cgccgtctct tcgcttgcga tgtagagccg gtcgccgcac tcgccgacga
     4021 ccataggccg gagtttgccc cggtcggtga acccgaccat catatcgggg ttcgccgcca
     4081 cgacggtgaa cggcccgttc atcgtcgcgg ggccgtaggc gaaccggagg g
//
LOCUS       DAOSII010000158         4127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_19075_length_4127_cov_47.005403, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000158 DAOSII010000000
VERSION     DAOSII010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19075_length_4127_cov_47.005403"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2033)
                     /gene="gyrA"
                     /locus_tag="PLV90_09340"
     CDS             complement(<1..2033)
                     /gene="gyrA"
                     /locus_tag="PLV90_09340"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOF97356.1"
                     /translation="MKSCYIDYAMSVIIGRAIPDARDGLKPVHRRTLYAMWEMGNTSD
                     KPYKKSARVVGDVMGKYHPHGDSAIYDTLVKMAQPFSYRYMLVDGQGNFGSIDGDSAA
                     AMRYTEARLTKVSEELLTDIEKETVDFVPNFDESLKEPDVLPARVPNLLVNGSSGIAV
                     GMATNMPPHNLREVCEATCRIIDEPGISTEELMRIMPGPDFPTGGVIMGTDGVRDAYL
                     TGRGKCVVRGIAEIEEEGRTPQIIITEIPFQVNKARLIEHIASLVRDKRIEGISDIRD
                     ESDRDGMRVVIDLKKGVAPQVVLNFLYKHTALESSFGIINLAIVDRQPRTLNLKELIQ
                     EFLKHRVDVVRRRSEFDLRKTEERMHILRGLLIALDNIDAVVATIRASQTTEEALAAL
                     VAKFALDEVQADAILKMQLRRLAALEQQKILDERDALAKEIERLTAILASEASILAEI
                     RKELIDISARYGDERRTRIGYAAEAFDKEDLIEDKPMLVSLTSSNYIKRIDLDTYRNQ
                     RRGGRGIIGMATKDDDGVENVFVANMHDCLLCFTDRGKVYWLKVYDLPEGQRTGKGKA
                     LVNLLNLDGGERVTAVIPVREFGSDHFLFFATRGGTVAKMALDEFSRPRQTGINAINL
                     RDGDELVDVKATDGNQELILTTRFGQSLRFHEEAVRPVRRNAMGVRGIRLRHG"
     gene            complement(2071..4041)
                     /gene="gyrB"
                     /locus_tag="PLV90_09345"
     CDS             complement(2071..4041)
                     /gene="gyrB"
                     /locus_tag="PLV90_09345"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOF97357.1"
                     /translation="MTDTYDASHITVLEGLRPVRERPAMYIGSTEARGLHHLVYEVVD
                     NSVDEALAGFCDLIRVVINRDGSVTVEDNGRGIPVDMMPQYGKSALEIVLTVLHAGGK
                     FDKNTYQVSGGLHGVGVSVVNALAGWLDATVFRDGNIYTMQFRQGQVAKPLTCRPETE
                     HEMRARYEERYGTRPGQHIDPIRLQGTRITFQPDRSIFETVEFDYDVLDHRLRELAYL
                     NSGLTIALQDDRTGDAVTYCFEGGIKEFVAHIGEGKEKLHDDVISFQKRDAESMVEVD
                     VALQYNDSYAETIYTYVNSVNTREGGTHLEGFRSAITRAINNAARRNNLLKSNDAQVR
                     GEDVREGLASVISTRVAEPQFEGQTKMRLGNSNVRGIVDSLVYSSLTEYFEEHPKTLQ
                     VIVDKAMTAARAREAARNARELARRKSTLETTGLPGKLADCQERDPAKSELYIVEGDS
                     AGGSAKQGRDRKFQAILPLRGKILNVEKASEHRILKNAEIQALISAIGTGVGDSFDAE
                     RARYHRIILMTDADVDGAHIRTLLLTFFYRYMPALVENGYIYIAQPPLYRVAKGKQER
                     YAYREEEMQEIAAGFGEKGVTIQRYKGLGEMNAGQLWGTTMDPKNRVLKQVRIEDAIY
                     ANDIFEKLMGENVDARRDFIRRHAKEVNNLDI"
BASE COUNT          658 a         1292 c         1276 g          901 t
ORIGIN      
        1 tcgccgtggc ggagccggat cccccgcacg cccatggcgt tgcggcggac cgggcggacg
       61 gcctcctcgt ggaaccggag gctctgcccg aacctcgtcg tcaggatcag ctcctggttc
      121 ccgtcggtcg ccttcacatc caccagttcg tcgccgtcgc ggaggttgat ggcgttgatc
      181 ccggtctgcc ggggccgcga gaactcatcg agcgccatct tcgcgaccgt cccgcccctc
      241 gtcgcaaaga agaggaagtg gtcggacccg aactcccgca ccgggatcac cgcggtcacg
      301 cgctctccgc cgtcgaggtt caggaggttg acgagggcct tgcccttgcc ggtccgctgc
      361 ccctcgggga ggtcgtagac cttcagccag tagaccttgc ccctgtcggt gaagcagagg
      421 aggcagtcgt gcatgttcgc gacgaagacg ttctcgacgc cgtcgtcgtc tttggtcgcc
      481 atcccgatga tgccgcgccc cccgcgccgc tggttccggt aggtatccag atcgatcctc
      541 ttgatatagt tcgatgaggt gagcgagacc agcatcggtt tatcctcgat gaggtcctcc
      601 ttatcgaacg cctccgcggc atacccgatc cgggtccgcc gctcatcgcc gtaccgggcg
      661 ctgatatcga taagctcctt cctgatctcg gcaaggatgc tcgcctcgct tgcaaggata
      721 gcggtgagcc gctcgatctc ctttgcaagg gcatcgcgtt catcgaggat cttctgctgc
      781 tcgagcgccg caagcctccg gagctgcatc ttcaggattg catccgcctg cacctcatcg
      841 agagcgaact tcgccaccag cgccgccagc gcttcttcgg tcgtttgaga tgcccggatg
      901 gtggcgacga cggcgtcgat gttatcgagc gcgataagaa ggccgcggag gatgtgcatc
      961 cgctcctcgg tcttcctgag atcgaactcg cttcgccgcc ggaccacgtc cacccggtgt
     1021 ttgaggaact cctggatgag ctccttgagg tttaaggtgc ggggctggcg gtcgacgatg
     1081 gcgaggttga tgatgccgaa cgaggactcg agtgccgtgt gcttgtagag gaagttcagg
     1141 acaacctgcg gcgcgacacc cttcttgagg tcgatcacga cccgcatgcc gtcccggtcc
     1201 gactcgtcgc ggatatcgct gatcccctcg atcctcttgt cgcggacaag ggaggcgata
     1261 tgctcgatca ggcgggcttt gttcacctgg aagggtatct cggtgatgat gatctgcggt
     1321 gtccggcctt cctcctcgat ctctgctatc cccctgacca cgcacttccc gcgcccggtg
     1381 aggtaggcat cccggacccc gtcggtgccc atgatgacac cgcccgtcgg gaagtccgga
     1441 ccgggcatga tccgcatcag ttcctcggtg gatatgcccg gttcgtcgat gatgcgacag
     1501 gtcgcctcgc agacctcgcg gaggttatgg ggcggcatgt tcgtcgccat accgaccgca
     1561 atcccgctcg acccgttcac gaggaggttt gggactcgtg ccgggaggac gtcgggctcc
     1621 ttgagcgatt catcgaagtt cgggacgaag tcgacggtct ccttctcgat atccgtcagg
     1681 agctcttctg agaccttcgt gagccgtgct tccgtgtacc gcatggcggc agcggagtcc
     1741 ccgtcgatcg acccgaagtt cccctggccg tccaccagca tgtagcggta ggagaagggc
     1801 tgcgccatct tcaccagcgt atcgtatatg gccgaatcgc cgtgggggtg gtacttaccc
     1861 atcacgtctc cgacaacacg ggcgctcttt ttgtagggct tgtcgctcgt gttgcccatc
     1921 tcccacatgg cgtagagggt gcggcggtgg accggcttca acccgtctct ggcatcgggg
     1981 atggcgcggc cgatgatcac gctcatcgcg tagtcgatgt agcatgactt catctcctcc
     2041 tcgatgttga ccgggacgac cccgacccgt tcagatatca aggttgttca cctccttagc
     2101 atgccggcgg atgaagtccc gccgggcatc gacgttctct cccatcagtt tctcgaatat
     2161 atcgtttgcg tagatggcat cttcgatccg gacctgtttg aggacccggt ttttgggatc
     2221 catcgtggtg ccccagagct gcccggcatt catctcgcca agacccttgt agcgctggat
     2281 cgtgacgccc ttctcgccga acccggcggc gatctcctgc atctcctctt cgcggtaggc
     2341 gtagcgctcc tgtttgccct ttgccacccg gtagaggggc ggctgggcga tgtagatgta
     2401 gccgttctcg acaagtgccg gcatgtagcg gtagaagaac gtgaggagga gcgtccggat
     2461 gtgcgcgccg tcgacatcgg catcggtcat caggatgatc cggtggtatc gggcccgctc
     2521 ggcatcgaaa ctgtcgccga caccggtccc tatggcggat atcagagcct ggatctcggc
     2581 gttcttcagt atcctgtgct ccgaggcctt ctcgacgttc aggatcttcc cccgcagggg
     2641 gaggatcgcc tggaactttc ggtcccggcc ctgtttggcg gaaccgcctg ccgaatcccc
     2701 ttccacgata tagagttcgc tctttgccgg gtcccgttcc tggcaatcgg cgagctttcc
     2761 ggggaggccg gtcgtctcga gcgtgctctt ccttcgggcg agctcgcggg cgttccgggc
     2821 ggcttcccgc gcccgtgcgg ccgtcatcgc cttatccacg atcacctgaa gggtttttgg
     2881 gtgctcctcg aagtactcgg tgagcgacga gtagacgagc gagtccacga tcccgcggac
     2941 gttgctgttc ccaaggcgca tcttcgtctg cccttcgaac tgcggttcgg cgacccgggt
     3001 gctgatgacg gaggcaagcc cctccctgac gtcttctccc cggacctggg cgtcgttgct
     3061 ctttaagagg ttgtttcgcc gggcggcgtt gttgatcgcc cgggtgatgg cgctccggaa
     3121 cccctcgagg tgggtgccgc cttcccgggt gttcacgctg ttgacgtagg tgtagatcgt
     3181 ctcggcgtat gagtcgttgt actgcagggc cacatcgacc tcgaccatgc tctcggcatc
     3241 acgcttctga aaggagatca cgtcgtcgtg gagtttctcc ttcccttcac cgatatgggc
     3301 gacgaactcc ttgatgccgc cctcgaagca gtaggtgacc gcatcgccgg tgcggtcgtc
     3361 ctgaagagcg atcgtgaggc cgctgttgag gtacgcgagt tcgcggagcc ggtgatccag
     3421 gacgtcgtag tcgaactcga cggtctcgaa gatcgaccga tcgggctgga acgttatccg
     3481 ggttccctgg aggcggatgg ggtcgatgtg ctgtcccggc cgggtgccgt accgctcctc
     3541 gtaccgggcc ctcatctcgt gctcggtctc cgggcggcac gtgagcggct ttgctacctg
     3601 gccctgccgg aactgcatcg tgtagatgtt gccatcccga aataccgttg catcgagcca
     3661 gcctgcgagg gcgttgacga ccgagacgcc gacgccgtgc aggcctccgg agacctgata
     3721 ggtgtttttg tcgaactttc cgccggcgtg aaggacggtg aggacgatct caagtgcgct
     3781 cttgccgtac tggggcatca tatcgacggg aatgccacgc ccgttatcct cgaccgtgac
     3841 cgagccgtca cggttgatga cgacccgtat caggtcgcag aacccggcaa gggcctcgtc
     3901 tacggagtta tccacaacct cgtagaccag gtggtgtaat ccccgggcct ccgtgctgcc
     3961 gatatacatg gcggggcgct cccgcacggg cctcaagccc tcaagtaccg tgatgtggga
     4021 agcatcgtag gtatcagtca tttatgacct ccgctgaacg agcggcgtcc tgaatcgcag
     4081 caaaactcac agtatatatt ggtcgtataa ccacttatag tgtatgg
//
LOCUS       DAOSII010000159         4109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_19179_length_4109_cov_45.514554, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000159 DAOSII010000000
VERSION     DAOSII010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19179_length_4109_cov_45.514554"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..>1382
                     /locus_tag="PLV90_09350"
     CDS             216..>1382
                     /locus_tag="PLV90_09350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOF97358.1"
                     /translation="MHKSDRAYAGLIGMSAIHGIRAYERYAGRETIRRIEAKARPLRD
                     MHILHMNSTYYGGGVSQILSSLTLLMNGLGIRTGWRVVHGPPDFFSVTKKFHNALQGA
                     DINLTDRKKEIYEGVIRENAMRNHLDHDVVFIHDPQPLPMITHYRKRSPWIWRCHVDL
                     TTPNQKVWNYFAPLIEKYDAVILSCKEYRQDLRTPQIFFMPAIDPFSIVNRELSESAI
                     DERLAYYNIPTDLPLVVQISRFDRWKDPLGVIQAFEKARKEVECTLVLVGNVATDDPE
                     GAEVYRSLLAHRNERVIILSVQDGALVNALQRRAAVVLQKSIREGFGLTVSEAMWKGA
                     AVIGGNVGGILHQIRDGENGFLVSSVDEAAGRIVQLLRDPDLARRLGHAAHETVR"
     assembly_gap    1383..1482
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1576..2325
                     /locus_tag="PLV90_09355"
     CDS             1576..2325
                     /locus_tag="PLV90_09355"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOF97359.1"
                     /translation="MAIQVIGVSGLPLIQKGDDLPALICGRVTLEDGDILTIASSVYS
                     KAKGFTRELSAITPGADAVRIAEKTKEDPRFVQAVLDSSTDILLEYPFILSEVPSGHI
                     GVRAGVDHSNIEDGRVIVLPPDPMAAAAEIRDAIRRITGKDVRVIITDTCGRSFRRGQ
                     TGIAIGWAGMTAINDYRGDTDLFGHVLQITEEAVVDEIAAFANFVMGESHQGVPAVVF
                     RNCKAWKGHDAVYFTPEEDIIRAALAKNKKD"
     gene            complement(2322..2480)
                     /locus_tag="PLV90_09360"
     CDS             complement(2322..2480)
                     /locus_tag="PLV90_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit Sec61beta"
                     /protein_id="HOF97360.1"
                     /translation="MAKKSGGRLVSSAGLVNYYDSEDHRAIHISPVAVIIVTAVIGVA
                     VFILNALF"
     gene            2525..3571
                     /locus_tag="PLV90_09365"
     CDS             2525..3571
                     /locus_tag="PLV90_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOF97361.1"
                     /translation="MQQVAPYTITGRALLGEDLEEEDVAITVSGGIVTAIEPYRGTPE
                     RWIVPAFFNAHTHLGDTVAMDLPARGSLAELVKPPDGLKHRILAATPRAELVRGMRSS
                     IKTMIATGTAGFADFREGGAAGVAAIREAAAGLDCRPVILGREGGERVSDGAGISSAH
                     DVKNAEEVVREARKIGRIVAFHAGEKNPDDIDDALAFDPDLLVHCTHATDAHLRRIVD
                     MGIPIAVCPRSNWLLGVAASPARPPIARILELGGRLLLGTDNVMFVQPDMLREMAFTA
                     TVYRAPPKEILHAAIAGATLAGRTGYLEIGQNTKMLVINPAKCNLSFSKDIRTTIVKR
                     LDPSSIEQNVLTLQ"
     gene            3608..4036
                     /locus_tag="PLV90_09370"
     CDS             3608..4036
                     /locus_tag="PLV90_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOF97362.1"
                     /translation="MFRKILVAIDGSEPASHALDAALDEARVRNAEVHVVYVIESGLF
                     SSLPMDNTLEIIYSVLQKEGEEILESAKKKADAKNIPLTTHLLQGHAGSRIASLAEEL
                     GVDLIVLGSYGKSGVDRLLLGSVTDYVVQNSPVTTLVVRS"
BASE COUNT          827 a         1258 c         1221 g          703 t
ORIGIN      
        1 gggtatccct tgccgcaggc accggcggga tgggggtgcc tgagaagtac atgggtatcc
       61 cttgccgcag gcgcgggcgg gggattgccg gcgaactgcg tatgcgtggc caggcggggt
      121 gaaacccgcg ttacggggat gaccagcggg gaacacccgg gtccggcacc cctcctcggg
      181 ggtgaggtcc ccgatctacg cgggggttcg ccggtatgca taagagcgat cgcgcgtatg
      241 cagggctgat cgggatgagc gcaattcacg gtatcagggc atatgagcgg tatgcaggaa
      301 gagagacgat acggcgtatc gaggcgaaag cccgcccgct ccgggatatg catatactgc
      361 acatgaactc cacctactac ggcggcggcg tctcgcagat cctctcctcc ctgacgctcc
      421 tgatgaacgg cctcggcata cggaccgggt ggcgcgtcgt gcacggcccg ccggacttct
      481 tcagcgtgac aaagaagttc cataacgccc tccagggagc ggatatcaac ctgaccgacc
      541 ggaagaagga gatctacgag ggggtcatcc gcgagaacgc catgcggaac cacctcgatc
      601 acgacgtggt cttcatccac gatccgcaac ccctcccgat gatcacccat taccggaaga
      661 ggagcccctg gatatggcgc tgccatgtcg acctcaccac accgaaccag aaggtctgga
      721 actactttgc cccgctcatc gagaagtacg acgccgtcat cctctcctgc aaagagtacc
      781 gccaggatct caggacgccg cagatcttct tcatgcccgc catcgacccc ttctccatag
      841 tgaaccggga gctctccgag agcgcaatcg acgagcgtct tgcgtactac aatatcccga
      901 ccgacctccc cctggtcgtg cagatctccc ggttcgaccg ctggaaagac ccgctggggg
      961 tcatccaggc gtttgagaaa gcgaggaaag aagtggagtg caccctggtc ctcgtcggaa
     1021 acgtggcgac cgatgatccg gagggtgctg aggtctaccg gtcactcctt gcccaccgga
     1081 atgaacgggt catcatcctg agcgtccagg atggggcgct cgtgaacgcg ctccagcgcc
     1141 gcgcagcggt cgtcctgcag aaatccatcc gggaagggtt cgggctgacg gtatcggagg
     1201 cgatgtggaa gggggcggcg gtgatcggcg ggaacgtcgg cggtatcctc caccagatac
     1261 gggatggaga gaacgggttt ctggtctcct ccgtcgatga agcggccggg cggatcgtcc
     1321 agctgctccg cgatcccgac ctggcgcgcc ggctcgggca tgcggcgcac gagacggtgc
     1381 ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncagggccg ctatcggcaa
     1501 aatccgccgg tatccttcca tagtaaaccc gagcattttt ctgcgagcgg gtgcgatcac
     1561 tgatctgtga gttccatggc tatccaggta atcggtgttt caggcctccc cctgatccag
     1621 aaaggcgacg acctgcccgc cctcatctgc ggccgggtca cgcttgaaga tggagatatc
     1681 cttacaattg cttcatcggt ctactcaaag gcaaaggggt tcacccgtga actctccgcc
     1741 atcactcccg gcgcggatgc cgtgcggatc gcggagaaga caaaggaaga cccccgattc
     1801 gtgcaggccg tgctggactc ttccaccgat attctgctcg agtacccgtt catcctctcc
     1861 gaggtgccgt ccggccacat cggcgtccgg gcaggcgtcg atcacagcaa catcgaggac
     1921 ggccgggtca tcgttctccc gcccgacccc atggcggccg ccgccgagat ccgcgacgcc
     1981 atccgcagga tcacgggaaa agacgtccgg gtgatcatca ccgacacctg cggcaggtcg
     2041 tttcggcgcg gccagaccgg catcgccatc gggtgggcgg gtatgacggc gatcaacgac
     2101 taccggggcg acaccgacct cttcggccac gtcctccaga tcaccgaaga agcggtggtc
     2161 gatgagattg cggcattcgc gaacttcgtc atgggcgaga gccaccaggg agtccccgcc
     2221 gtggtcttcc ggaactgcaa ggcctggaaa gggcacgacg ccgtctactt cacgcccgaa
     2281 gaagacatca tcagggccgc cctcgcaaag aacaaaaagg attagaagag ggcattgagg
     2341 ataaagactg ccactccgat caccgcggtc acgatgatca cggccaccgg gctgatatgg
     2401 atggcccggt ggtcctcgct gtcgtagtag ttgaccaggc cggcagagga taccagtctt
     2461 ccgccagatt ttttcgccat acagaaatat actcctttgc catatataaa acaaaggtcg
     2521 aacgatgcag caagtcgcac cgtataccat caccggccgg gcgctcctcg gcgaagacct
     2581 cgaggaggaa gacgtcgcca tcaccgtctc ggggggtatc gtcaccgcga tagaacctta
     2641 ccggggaacg ccggaacggt ggatcgtgcc cgccttcttc aacgcccaca cccacctcgg
     2701 cgacaccgtc gcgatggatc tccccgcccg gggcagcctt gccgaactcg tcaaaccccc
     2761 ggacggcctg aaacaccgga tcctcgcggc aaccccgcgg gccgaactcg tcagggggat
     2821 gcggtcgagc atcaagacga tgatcgcgac cggaacggcc gggtttgccg acttccggga
     2881 agggggggcc gccggtgtcg cggcgatccg cgaggctgcg gccgggctcg actgccgccc
     2941 ggtcattctc ggccgcgaag gcggggagcg ggtgagcgac ggggcgggca tcagcagcgc
     3001 ccacgatgtg aagaacgccg aagaggtcgt cagggaggcc cggaagatcg gccggatcgt
     3061 cgcctttcat gccggggaga agaacccgga cgacatcgac gatgcgctcg cgttcgatcc
     3121 cgacctgctc gtccactgca cccacgcgac ggatgcgcac ctccgccgga tcgtcgatat
     3181 ggggatcccg atcgccgtct gcccgcgatc gaactggctc ctcggggttg ccgcgtcgcc
     3241 ggcccgccca cctatagcgc gcatactcga actcggcggc cggctcctcc tcgggacgga
     3301 taacgtaatg ttcgtccagc ccgacatgct ccgggagatg gcttttaccg caaccgtcta
     3361 ccgcgcgccc ccaaaagaga tcctgcatgc cgcgatcgcg ggcgcaacgc tcgccggcag
     3421 gaccggatac ctggagatcg ggcagaatac caaaatgctc gtcataaacc cggcaaaatg
     3481 caacctctcc ttcagtaaag atatccggac gaccattgtg aagcgactgg atccatcctc
     3541 catcgaacaa aatgttttaa ccctgcagta gaaatctgat aagaagtatc tgttctgcgt
     3601 ggtgtgtatg ttccgtaaga tactcgttgc tatagatgga tccgaaccgg ccagtcatgc
     3661 gctggatgcg gccctcgatg aggcccgtgt ccggaatgcc gaggttcatg tcgtgtatgt
     3721 gatcgaatcc gggctgtttt catcccttcc catggacaac acactcgaga taatctacag
     3781 tgtcctgcag aaggaaggcg aggagatcct cgaatccgcc aaaaagaagg cagatgcaaa
     3841 gaatatccca ctcaccacgc acctcctgca ggggcatgcg ggatcgcgga tcgcttccct
     3901 cgccgaagag ttaggagtcg atctcatcgt cctcggatct tacggcaaga gcggcgtcga
     3961 tcgccttctc ctcgggagcg tgacagacta tgtcgtccag aacagccccg tcacgaccct
     4021 ggtggtgaga tcataacatc ctcgcgacag atgcttgtcc gtgacttcat ggtgaccgac
     4081 gtcgtctgcg tcgagattcc cgggaacag
//
LOCUS       DAOSII010000160         4091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_19269_length_4091_cov_59.858523, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000160 DAOSII010000000
VERSION     DAOSII010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19269_length_4091_cov_59.858523"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..191
                     /locus_tag="PLV90_09375"
     CDS             15..191
                     /locus_tag="PLV90_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOF97363.1"
                     /translation="MLRINRDKCGYCGTCVAVCPEDALELIDAYLSLERECIACGICA
                     RACPFGALEVVHEE"
     gene            181..1377
                     /locus_tag="PLV90_09380"
     CDS             181..1377
                     /locus_tag="PLV90_09380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOF97364.1"
                     /translation="MKSNYDILVVGGGPAGALAAKTAAEAGNSVCLIEKRSAIGTPVR
                     CAEGIGKELLREFVKPDPRWISAEIERARLIAPNGTTVNLEQDRAGNEVGYVLDRKIF
                     DRELVWQAAEAGADVVVKTRATAPIQENGVVRGAKVLSVGTPADIRADVVIAADGTEA
                     QFARRAGLDTTVPLREMMSCAQYLMTDIDIDAGSTDFYLGNDVAPEGYLWIFPKGERT
                     ANVGIGISGRKSRDGTRAKDYLDRFVAKNFPDGKTIECIAGGVPVCRPLPCTVGDGLM
                     VIGDAARVVDPLTGGGIGNAMFTGRLAAQVASECISAGNCSKEALMPYDAGWRASRMG
                     VLLERNYRVKEYFVTLDDRKLNTLAESIANISLKEFSVSALIKELIKRNPKMLLELKA
                     LKDALS"
     gene            complement(1369..2034)
                     /locus_tag="PLV90_09385"
     CDS             complement(1369..2034)
                     /locus_tag="PLV90_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOF97365.1"
                     /translation="MYTIIVGLGGIGRNLTAIAVNAGDSVVVIDQDEARASDILEHYD
                     VLAITGNATDKSVLQDAGIDRADALVATTSDDAVNLMTCWLAKRYNVRNVVSIVNQKE
                     HSDLFNEVGVKISENPDELVASRLYYWAKSPNLQQVASIPGGTIFEIVAEEGAPIVDH
                     EIRDLDVRDFVFIAIRRAGGDLIIPSGTVRIRPGDVITVFTKKEAEGETVKTLSKQLK
                     RSG"
     gene            2089..2718
                     /gene="rnhB"
                     /locus_tag="PLV90_09390"
     CDS             2089..2718
                     /gene="rnhB"
                     /locus_tag="PLV90_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOF97366.1"
                     /translation="MICGVDEAGKGSVIGPMVVAAVGCRAVEDLAALPIRDSKALRPK
                     QREELYGLIFRDFAIAIVTIDAAGIDEARSRMSMNACVAELHAEALMGLRPEHAYVDA
                     CDVNAERYGRTVTSHLDFPCAVVAEHRADARHKIVGAASVIAKVTRDRAIRELEQQYG
                     RIGSGYPSDPATIEFLRNYMREHGNPPPCARRSWKTVANLYQATMQDYL"
     gene            2759..4090
                     /locus_tag="PLV90_09395"
     CDS             2759..4090
                     /locus_tag="PLV90_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOF97367.1"
                     /translation="MTWLQILLLLVAAYLLIAYYIRDRGLFRDHVMFFGPIMAIKTER
                     VGFFDWFRKFQMPLRAYATLGVIMVVLVSAFMTLALLLAVPQLLVHTPEPTGIYDPRN
                     ILAIPGVNQAIPVTAAVIIGLVLTIVIHEFGHAILARVEGMRVRSMGLLIAVIPIGAF
                     VEPDEEDVDAAKGMPKIRMFGAGITNNIVIGLACFAVMFLLVGMATPLAVPLVQGVYK
                     DFPAADAGLPGYSVITSINGVPVASQAEVSAAMEGTRPGETVTLTAEKDGIESTYTLT
                     LSRWPEVLDGDHDSGFMGVYYYSAPVVKEHIGNIANLGLLAPLYLTIAPIDAFIQGNT
                     QQLAILLTDTPEQMAWKEPFPFFWGMVHLAFWTGWFNLLVGMFNAIPIVPLDGGYMMK
                     EGVERFLERRGWSRYAPRVVASISGFVTVMLILLITMPMLVAAVRFVLMMG"
BASE COUNT          755 a         1279 c         1296 g          761 t
ORIGIN      
        1 acaacatgag tacaatgctc aggataaacc gagacaagtg cggatactgc ggcacctgcg
       61 tcgcagtctg cccggaggat gcgctcgagc tgatcgatgc ctacctcagc cttgagcggg
      121 agtgcatcgc ctgcggcatc tgcgcacggg cgtgcccgtt cggggcactg gaggtcgtcc
      181 atgaagagta actacgatat tctcgtcgtc ggtggcggac ccgccggcgc tcttgcggca
      241 aagacggcgg cagaagcagg gaactcggtc tgcctcatcg agaagcgctc cgccatcggc
      301 acaccggtcc ggtgcgcgga ggggatcggc aaagaactct tacgggagtt cgtcaaacca
      361 gacccccgct ggatatcggc ggagatcgag cgtgcccggc tcatcgcccc gaacggcacc
      421 accgtcaacc tcgaacagga ccgggcggga aacgaggtcg gctacgtcct cgacaggaag
      481 atctttgacc gggaactcgt ctggcaggcg gccgaggcag gggcggacgt ggtcgtcaag
      541 acccgggcga ccgcgccgat ccaggagaac ggcgtggtcc ggggcgcaaa agtgctctcc
      601 gtcgggacgc cggccgacat ccgggccgac gtggtcatcg ccgcggacgg cacggaggcg
      661 cagttcgccc gccgggccgg cctcgacacc accgtgcccc tccgcgagat gatgagctgc
      721 gctcaatacc tgatgaccga tatcgatatc gatgcgggtt cgacggactt ctacctcggt
      781 aacgatgtcg ctcccgaagg ctacctctgg atcttcccga agggcgagcg gacggcaaac
      841 gtcgggatcg gcatctccgg caggaagagc cgggacggca cgcgggcaaa ggactacctc
      901 gaccggttcg tcgcaaagaa cttccccgac ggaaagacga tcgagtgcat cgcaggcggc
      961 gtcccggtct gccggccgct cccctgcacg gttggcgacg gccttatggt catcggcgac
     1021 gcggcaaggg tcgtcgaccc tctcaccggc ggtgggatcg ggaacgccat gttcaccgga
     1081 aggctggcgg ctcaggtggc ctccgaatgc atatcggcag gcaactgctc gaaggaggcg
     1141 ctgatgccct atgatgccgg gtggcgggca tcgcggatgg gcgtacttct cgagcggaac
     1201 tacagggtca aggagtactt cgtcacgctt gatgatagga aactcaacac gctcgcggaa
     1261 tcgatcgcaa acatcagtct caaggaattc tcggtctcgg cgctcatcaa ggagctgatc
     1321 aagcgcaacc cgaagatgct cctcgaactg aaagcgctca aagatgcttt atcctgaccg
     1381 cttcagctgt ttgctgaggg tctttaccgt ctccccctcc gcctcctttt ttgtaaatac
     1441 cgtgatgacg tctcccgggc ggatacggac ggtgccgctc ggtatgatga ggtcgccccc
     1501 ggcgcgccgg atggcgatga agacgaagtc gcggacgtcg aggtcgcgga tctcgtggtc
     1561 gacgatggga gcgccctctt cagcgactat ctcgaatatg gtgcctccgg ggatcgatgc
     1621 aacctgctgg aggtttgggc tctttgccca gtaatagagc cggctcgcca ccagttcgtc
     1681 ggggttttcg ctgatcttca ccccgacctc gttgaaaagg tcggagtgct ccttctggtt
     1741 aacgatcgag acgacgttcc tcacgttgta acgtttggca agccagcagg tcatcaggtt
     1801 cacggcgtcg tcgctcgtgg tggcgaccag agcgtcggca cggtctatcc cggcatcctg
     1861 cagcaccgac ttgtcggtcg cgttcccggt gatggcgaga acgtcgtagt gctccaggat
     1921 atcgctcgca cgcgcctcgt cctggtcgat caccaccacg gagtcgccgg cgttcacggc
     1981 gatcgcagtc aggttccggc cgatcccccc aaggccgacg atgatggtgt acattccatc
     2041 tgggttcgac cgttgccatt gaaatacctt gcgaataaac cagagtacgt gatctgcggg
     2101 gtggatgaag cggggaaggg ttcggtcatc ggcccgatgg tggtcgccgc cgtcgggtgc
     2161 cgggcagtcg aggaccttgc ggccctcccc atccgggact caaaggccct ccggccgaaa
     2221 caacgcgagg aactgtacgg gctcatcttc cgcgacttcg cgatagccat cgtcaccatc
     2281 gacgccgccg ggatcgacga ggcccggagc cggatgagca tgaacgcgtg cgtggccgaa
     2341 ctccacgccg aggcgctcat ggggctccgc ccggagcatg cctacgtgga cgcctgcgac
     2401 gtgaacgccg agcgttacgg cagaacggtc acgtctcacc tcgacttccc ctgtgcggtc
     2461 gtcgccgagc accgggcgga tgcccgccac aagatcgtcg gcgcggcgag cgtcatcgcg
     2521 aaggtcaccc gggaccgggc gatccgggaa ctcgagcagc agtacgggag gatcgggagc
     2581 ggctacccgt ccgatccggc aaccatcgag tttctccgga actacatgcg tgagcacggg
     2641 aacccgccgc cctgcgcccg ccggagctgg aagacagtgg cgaacctcta ccaggcgacg
     2701 atgcaggact acctgtagcc cggaagaatt tatgctttct gcgcgctaat cgtgagtgat
     2761 gacctggctc cagatactcc tccttctcgt cgcggcctat ctcctgatag catattatat
     2821 ccgcgaccgg ggattgtttc gcgatcacgt catgttcttc ggccccatca tggcgatcaa
     2881 gaccgagcgg gtcggtttct tcgattggtt ccggaaattc caaatgcctc tgcgggccta
     2941 cgcgacgctc ggggtcatca tggtcgtcct ggtctcggcc ttcatgacac tcgccctgct
     3001 ccttgcagtc ccccagctcc tggtgcatac ccccgaaccg accgggatct acgatccgag
     3061 aaacatcctc gccataccgg gggtcaatca ggcaataccg gtcaccgcgg ccgtcatcat
     3121 cggcctcgtg ctcacgatcg tcatccacga gttcggccac gcgatcctcg cccgggtcga
     3181 gggtatgcgg gtgcggagca tgggtctcct catcgccgtc atcccgatag gggcgttcgt
     3241 cgagcccgac gaagaggacg tcgatgcggc aaaagggatg ccgaagatcc ggatgttcgg
     3301 tgccggaata acgaacaaca tcgtcatcgg cctcgcctgt ttcgccgtga tgttcctcct
     3361 cgtcggtatg gcgacgcccc ttgccgtccc gctcgtccag ggggtctaca aggatttccc
     3421 ggcggccgac gcgggcctcc ccggctactc tgtcatcacg agcataaacg gtgtacccgt
     3481 ggcaagccag gcggaggtct cggcggcgat ggaggggacg cggcccgggg agacggtcac
     3541 gctgacggca gaaaaggatg ggatcgagag cacctacacc ctcaccctct cgagatggcc
     3601 ggaggtgctc gacggcgacc acgattcagg gttcatgggg gtctactact acagcgcccc
     3661 ggtggtgaag gagcatatcg ggaacatcgc aaacctcggg ctgcttgccc cgctctacct
     3721 gacgatagcc ccgatcgatg cgttcatcca gggcaacacc cagcagcttg ctatcctgct
     3781 taccgatact cccgagcaga tggcctggaa ggagccgttt cccttcttct ggggcatggt
     3841 tcacctcgcc ttctggacgg ggtggttcaa cctgctggtc ggcatgttca acgccatccc
     3901 gatcgtcccc ctcgacggcg gttatatgat gaaagaaggg gtggagcggt tcctcgagcg
     3961 ccggggctgg tcccggtacg caccaagggt cgtcgcctcg atcagcggat tcgtcacggt
     4021 gatgctgatc ctcctcatca cgatgccgat gcttgtcgcg gcggtgcggt ttgtgctgat
     4081 gatggggtga a
//
LOCUS       DAOSII010000161         4081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_19333_length_4081_cov_45.699454, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000161 DAOSII010000000
VERSION     DAOSII010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19333_length_4081_cov_45.699454"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /gene="glmM"
                     /locus_tag="PLV90_09400"
     CDS             <1..1305
                     /gene="glmM"
                     /locus_tag="PLV90_09400"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOF97368.1"
                     /translation="ETMTPALVLKIGAALGSMRKGTIAVGRDTRTSGEALAHALKAGL
                     LMTGCDVVDMGILPTPALQYIIKTGRFSGGAMITASHNPPEYNGVKVIEADGTEMSDE
                     EIIRLEDRFFAGEFEAASWEGVGAETAAPDRLEEYIEAVVRHFPAGIGEGMTVVVDPG
                     SGPAALTTPIILSRMGCRVHTINARLDGTFPGRMPEPTPEGLRPLSEMVVATGADFGV
                     AHDGDADRAVFVDNKGCYIEENYEFGLIEDYVCARNGGGLVVTPVATSRLIRDIAEKH
                     GCTVDYTPVGSIYVARRMIELIGGGKKVSFGGEGNGGLIYSDHQFCRDGGMTAAMMVA
                     VLASHNGRRLSDIQGDLPLYHLVKEKHHTHDPAALVRAIEEAFAGEAIEKIDGIKIVR
                     DDAWALVRASGTEPMIRVMIESRDPDIANAMYREIVQVVGRV"
     gene            1541..>4081
                     /locus_tag="PLV90_09405"
     CDS             1541..>4081
                     /locus_tag="PLV90_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97369.1"
                     /translation="MKQKITSAISSGDPRELEKVVLDISETQTRKEKKSLYEQMNAAL
                     VTAAFEGGQPELLSQLVEPTKEEIFDLVRRAAGLYSQNKDDIWFGAIFSLVNKLDRKS
                     HQSDILAEISRSLVQTGVDTGDIHYIEKGGEAFDKISIRKYRSAILSEIIPLLIQYGQ
                     KYQNIDIMRHALLMLSEIGDISRQSQLHADVARAIAGSGIESGNIALVISGLSSATEI
                     NQKIRRTNSIADIVDATWKSSQKKEIADIEQIIDALPDVPEERLTEVLAILTEQLLDR
                     QRDKKQVYARLIRIDDERPWAGQTLILELLKKAERSGERWYLEKAFEFNTRSTVEAKL
                     PIEEIVLSGIAVVEKSGNSTILLDIMPLIDESCDAMKAAQLYRQITDALSRMGDFYHA
                     IEVMKKASTSAQRINAQFFDTSVRLIKEGIRRGEVDLVRENILATLDIDIAESLVHQA
                     VTDFCKEYDFDEVVRHIPAVKELVSSHRTQDHLLIECNTILIERGFIRERDPSALVDL
                     VSQIGEQALREQAISTISVEMARIGVARKDRDLLRHATGLTCEIVDQQTRAEAMGGIV
                     DQAAVLAVEDGDLELLHGMRVLSTALLEREYCLFAMGKVIHGLIMYGIEQLSLQALDE
                     AAQILSQITDPSLQQQLVDPLTEGYVRVGSLQAADQLAGGKARIFEGMMEPFTTALHL
                     LQTSTPREEASIRIASYVDIMLEYTQVYRSPIFAVPMALFTLEIGGEYERTAMIQRIL
                     TFFTSYTKEFDSADPYEVMAYLLEGIEGATAAPPSLELMHRLFEHTGDVYARYSGMYR
                     IVSAYSGLGDEGQAREIIQRLHETIGTIGESSIRAIMLSDLAGLMAGID"
BASE COUNT          892 a         1261 c         1236 g          692 t
ORIGIN      
        1 gagaccatga cgccggccct cgtcctcaag ataggcgccg cgctcggatc gatgagaaag
       61 ggcacgatag ccgtcgggag agatacacgg acgtccggcg aggccctggc tcacgccctc
      121 aaggccgggc tcctcatgac cgggtgcgac gtggtggata tgggcatcct ccccacgccg
      181 gccctgcagt acatcatcaa gaccggccgg tttagcggcg gcgctatgat caccgcatcc
      241 cacaacccgc ccgagtacaa cggcgtgaag gtcatcgagg ccgacggcac cgagatgtcc
      301 gacgaagaga tcatccggct cgaggaccgg ttctttgccg gcgagttcga ggcggcctcc
      361 tgggaaggcg tcggcgcgga gaccgccgct cccgaccggc tcgaggagta catcgaggcc
      421 gtcgtacggc acttcccggc gggcatcggg gaggggatga ccgtcgtcgt cgatcccggc
      481 tccgggcccg cggcgctcac caccccgatc atcctctcga ggatgggctg ccgggtccac
      541 accatcaacg cccgtctcga cggcaccttc ccgggccgga tgcccgagcc gacccccgaa
      601 ggcctgcggc ccctctcaga gatggtcgtc gcgacgggcg ccgactttgg agtggcgcac
      661 gacggcgacg ccgaccgggc ggtcttcgtt gataacaaag gatgctatat agaagagaac
      721 tacgagttcg ggctgatcga agattacgtc tgcgccagga acggcggcgg gctcgtcgtc
      781 acccctgtcg ccacatcccg gctgatccgg gatatcgcgg agaaacacgg gtgcaccgtc
      841 gactacaccc ccgtggggag catctacgtc gcaaggagga tgatcgagct catcggcggg
      901 ggaaagaagg tctcgttcgg gggagaagga aacggcgggc tgatatactc cgaccaccag
      961 ttctgccggg acgggggcat gaccgccgcc atgatggtgg cggtccttgc aagccataac
     1021 ggcaggaggc tctcggatat ccagggcgac ctccccctct accacctggt caaggagaag
     1081 caccacaccc acgacccggc cgcactggtc cgtgccatcg aggaagcgtt cgcgggggag
     1141 gccatcgaga agatcgacgg catcaagatc gtcagggacg acgcctgggc gcttgtgcgg
     1201 gcatcgggca cggagccgat gatcagggtc atgatcgagt caagggatcc ggatatcgcg
     1261 aacgccatgt accgggagat cgtgcaggtc gtcgggcggg tgtgatcacc cgcccgcgtg
     1321 atccccggga acagatgaaa ccatcacaca ttaagtgttc cattcacggt ggcacggcgg
     1381 gcctgtatcg tccgccatat ctccgacatt tttcaggatc tccccgattc cccttgaaaa
     1441 acattcttgt gggtgggggg caatctatat atctgataat ggacatgcat gatgcagaac
     1501 ccactggaga gcgctgatca aaatggatgg aaaaccccag atgaaacaga agatcacctc
     1561 cgcaatctct tccggggatc cgcgtgagct tgaaaaggta gtgctggaca tatcggagac
     1621 ccagacacgg aaagagaaga aatcgctcta tgagcagatg aatgccgccc tggtaacggc
     1681 agccttcgag gggggacagc ccgaactctt gagccagctc gtcgaaccga cgaaagaaga
     1741 aatatttgac ctcgtaagac gtgccgcagg gctttatagc cagaataaag acgacatctg
     1801 gtttggtgcc atcttctccc tggtcaataa gctcgaccgc aagagccacc agtccgacat
     1861 actcgccgag atctcccgca gtctcgtcca gaccggagtc gataccggag atatccacta
     1921 tatcgagaag ggaggagaag cgttcgataa gatcagcatc cgtaaatatc gctcggcaat
     1981 cctctccgag atcataccgc tcctcatcca atacggacag aaataccaga atatcgatat
     2041 catgcgacat gccctcctca tgctctccga gatcggcgat atatcaagac agtcacagct
     2101 ccatgccgac gtggcccggg cgattgccgg ttccggcatc gaatccggca acatcgccct
     2161 cgtgatcagc ggactatcga gcgccaccga gatcaaccag aagatccggc gcaccaactc
     2221 catcgccgac atcgtggatg ccacgtggaa gtcgtcgcaa aagaaggaga tcgctgatat
     2281 cgagcagatc atagatgccc tcccggatgt cccggaggaa cggctcaccg aggtcctcgc
     2341 gatcctgacc gagcaactcc tcgaccgtca gcgggacaag aaacaggtct acgccaggct
     2401 gatcaggatc gacgacgaga ggccgtgggc gggccagacc ctcatactcg agctcctgaa
     2461 gaaagcggag cggagcggag aacgctggta ccttgagaag gcgttcgagt tcaacacccg
     2521 gagcacggtg gaggccaaac tccccatcga ggagatcgtc ctctccggga ttgcggtcgt
     2581 cgagaagagc ggcaactcga ccatcctcct cgacatcatg ccgctgatcg atgagtcctg
     2641 tgacgcaatg aaggcggccc agctctaccg gcagatcacc gacgccctct cccggatggg
     2701 cgatttctac cacgcgatcg aggtcatgaa gaaagccagc accagcgccc agaggatcaa
     2761 cgctcaattt ttcgatacca gtgtccgcct gatcaaggag ggtatccggc gcggcgaggt
     2821 cgacctcgtc cgcgagaata tcctcgccac actggacatc gatattgccg aatcgctggt
     2881 gcaccaggcg gtgaccgact tctgcaagga gtatgacttc gatgaggtcg tcaggcacat
     2941 accggccgta aaggaactcg tgagctcgca caggacacag gaccacctgc tcatcgagtg
     3001 caacaccatc ctgatcgagc gggggttcat ccgggagaga gatccgtcgg ccctcgtgga
     3061 cctggtgagc cagatcggcg agcaggctct ccgcgaacag gcgatatcga ccatatccgt
     3121 cgagatggcg cgtatcggcg tcgcccggaa agatcgggat ctcctccggc atgcgaccgg
     3181 gctcacctgc gagatcgtgg accagcagac acgggcggag gcgatggggg gcatcgtgga
     3241 ccaggcggcc gtgctcgccg tggaggacgg cgacctcgaa ctgctccacg gtatgcgggt
     3301 cctctccacg gccctgctgg agcgggagta ctgcctcttt gcgatgggca aggtcatcca
     3361 cggcctgatc atgtacggga tcgagcagct ctcgctccag gccctggacg aagcagccca
     3421 gatcctctcc cagatcaccg atccctccct ccagcagcag ctggtcgatc ccctcaccga
     3481 gggctacgtc cgtgtcggga gcctgcaggc ggcagaccag cttgccgggg gaaaggcccg
     3541 gatattcgaa ggcatgatgg aaccgttcac gaccgccctg cacctcctcc agaccagcac
     3601 cccgcgcgag gaggcctcga tcaggatagc aagttacgtc gacatcatgc tcgagtacac
     3661 gcaggtctat aggagtccga tctttgccgt ccccatggct ctcttcacgc tcgagatcgg
     3721 gggagaatac gaacggactg ccatgatcca gcggatactc acgttcttca ccagttacac
     3781 gaaggagttc gattcggccg acccctacga ggttatggct tacctgctcg agggtatcga
     3841 gggggcgacg gcagcgccgc cgtcacttga gttgatgcac cgcctctttg agcataccgg
     3901 cgacgtctat gcccggtact cggggatgta ccggatcgtg agcgcctact caggccttgg
     3961 cgacgaaggg caggccaggg agatcatcca gcggctccac gagaccatcg gcaccatcgg
     4021 cgagtcatcc atccgtgcga tcatgctctc cgaccttgcc ggcctcatgg cagggatcga
     4081 t
//
LOCUS       DAOSII010000162         4045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_19566_length_4045_cov_49.762907, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000162 DAOSII010000000
VERSION     DAOSII010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19566_length_4045_cov_49.762907"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            239..>1594
                     /locus_tag="PLV90_09410"
     CDS             239..>1594
                     /locus_tag="PLV90_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOF97370.1"
                     /translation="MLFRKIKSDILAHNSYMIGADGEAAVIDPRRDCQVYTSVAGRRD
                     MKIAAIFETHKNEDYVSGSRELARPTGAEILHGAREEFGFGTPVREGETFEFGSLEIE
                     VLETPGHTIESISLLLRDRSVSDDPLMVFTGDALFAGDVGRTDLAGEDRRREMSEMLY
                     ESLHEKLLPLPDGVIVCPAHGAGSVCGGNISDREYTSIGFERATNRLLSMGKEEFVEY
                     KVNERLYEPPYFTMMRRLNKAGPPLIYNLPYLRPYTARELRSLLDRKAQVVDIRSPTG
                     FAGGHVPGTINIWRNGLPLYVGYYLNYEDPIVLVDDFNLGMGHVLRHFVRLGYDNIGG
                     YLAGGFPAWFKQGERIGRSGIWSPSDLAERLNDPSIYILDVRKITHREESGHIRGSHH
                     IYVGNLEERIKEIPEDKEIVVHCDAGFKGSLAASILKKHGFRHVANLLGGTIGWEAAN
                     YP"
     assembly_gap    1595..1694
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1748..2221)
                     /locus_tag="PLV90_09415"
     CDS             complement(1748..2221)
                     /locus_tag="PLV90_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HOF97371.1"
                     /translation="MEEVTLDRDDIAALSSDARITILKLLDTRTMTMSELAEGLGLAK
                     STVHEHLAILTDADLVTHANARKWRDYTLTKRGRRVLHPGKDHRIIFLFGTSLATVTA
                     GALLVASFLRGFAVQGGNVVRDPFLLCAGEVLLGATIVLWYLALRCRRRTTPTPG"
     gene            complement(2348..3172)
                     /locus_tag="PLV90_09420"
     CDS             complement(2348..3172)
                     /locus_tag="PLV90_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97372.1"
                     /translation="MHPKTWIWLGIGVVLLLAAANLAILAAFPPGQQHVQEPVKRVDW
                     ILPFGPGFEYRGPYESPVIAYDGANETWHLRNVTAYNRTINLGDRYSLDPAHPEDIRY
                     TPDVAIEGGQVSLDFLIRNLAGEDCARADLAMTTGTTTLNTTTGSPEGNIPPGAATTL
                     PIDDLAAGEWREVRVTADLPRPGQEEVLYLAIDLTAPYTLTTPNGTPVDFDFRRETVT
                     LLGSNTSWKEEIDPGSTPDPDYNRARLPRGSATFIIMGIDRSEPIPPPTFPTLRAG"
     gene            3507..3938
                     /locus_tag="PLV90_09425"
     CDS             3507..3938
                     /locus_tag="PLV90_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97373.1"
                     /translation="MTPLHHEKSARLRRYLIAVAAAGFVLLGCYVVVSSYIAATIPPD
                     AFLLYEVTEETTQKGIVIRLDEEEFRNLPVLDAFLRDEEHNTEGEPYAPGDIRLVGSV
                     DYPEKIRQETIDAYIFDSETGRRKYFEYEGRYYRVGTIYRD"
BASE COUNT          761 a         1146 c         1280 g          758 t
ORIGIN      
        1 tgcgcatcct caggagttgc cggatcccga acttcgcaga gttagaggat gcgttcaagg
       61 ccgtgggaac catcctctcg gtcaggaccg cacggtcggg cgatctgccc cgccttccca
      121 aacctgactg gtgcgcgggc ggcgagcggt agcgcccgca ctcccgttaa ccttttatac
      181 acccctcccg catcccggca tatcacgacg ggttctgcca cccgggagat gcatacggat
      241 gctgtttaga aagataaagt ccgatatcct tgcgcataac tcatacatga tcggagcgga
      301 cggcgaggcg gcggtcatcg atccgaggcg tgactgccag gtctatacga gcgtcgccgg
      361 gcgccgggat atgaagatcg cggcgatctt tgagacgcac aagaacgagg attacgtcag
      421 cggctcccgg gagctcgctc gtccgaccgg agcggagatc ctgcacgggg cgcgggagga
      481 gttcgggttc ggcacccctg tgcgggaagg ggagacgttt gagttcggat cgctcgagat
      541 tgaggttctg gagacgcccg gccacaccat cgagagcata tcgctcctgc tgcgtgaccg
      601 ctcggtctcc gacgatcccc tgatggtctt taccggcgat gcactctttg cgggcgacgt
      661 cgggagaacc gatctcgccg gcgaggaccg ccggcgcgag atgtcggaga tgctgtacga
      721 gagcctgcac gagaaactcc tcccgcttcc cgacggggtg atcgtctgcc ccgcccacgg
      781 tgccggctcg gtctgcggcg ggaacatcag cgaccgcgag tacacatcca tcggttttga
      841 gcgggcgacg aaccgtctcc tctccatggg gaaggaggag ttcgtcgagt acaaggtgaa
      901 cgagcgcctc tacgagccgc cgtattttac gatgatgcga agactcaaca aggccggccc
      961 gcccctgata tacaacctgc cgtacctgcg gccgtatacg gcccgcgagc tccgttctct
     1021 ccttgatcgg aaggcgcagg tcgtcgacat acggtcgccg acgggttttg ccggcggaca
     1081 tgtcccgggc accatcaaca tctggcgcaa cggcctccct ctctacgtgg gctactactt
     1141 aaactacgaa gacccgatcg tcctcgtcga cgactttaac ctcgggatgg gccacgtact
     1201 gcgccatttc gtgcggctcg gctacgacaa catcggaggt taccttgccg gcggatttcc
     1261 cgcctggttc aaacagggcg agcggatcgg gcggtccggg atctggtcgc cctccgacct
     1321 cgcggagcgc cttaacgacc cgtcgatcta catcctcgac gtgcgcaaga tcacccaccg
     1381 ggaggagtcg ggccacattc gaggctccca ccacatctac gtcggcaacc tcgaagagcg
     1441 gattaaggag attccggagg acaaagagat cgtcgttcac tgcgacgccg ggttcaaggg
     1501 gagccttgcg gcaagcatcc tcaagaagca cgggttccgg cacgtcgcaa acctgctcgg
     1561 gggcaccatc gggtgggaag cggcgaacta cccgnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnncgggaa ttgcggttgg gtctgtcaag atacagtacc gtcgatagac
     1741 gggcgatcta cccgggtgtc ggagtggttc tccgccggca ccgcagcgca aggtaccaga
     1801 gcacgattgt cgcgccgagg agcacctcgc ccgcgcagag gaggaaggga tctcggacga
     1861 cgtttccccc ctgcacggcg aaaccgcgga gaaacgaggc cacgagaagt gccccggccg
     1921 tcacggtagc aagggacgta ccgaagagaa agatgatccg gtggtccttt ccgggatgaa
     1981 gaacccgccg ccctctcttc gtcagggtgt agtcccgcca tttccgggca ttcgcgtgcg
     2041 tcaccaggtc cgcgtcggtg aggatggcga ggtgctcgtg aacggtcgac tttgcaagac
     2101 cgagtccttc ggcgagctca ctcatcgtca tcgtccgggt atcgagcaat ttaagaatgg
     2161 tgatccgggc gtcggaggag agagcggcga tgtcatcccg gtcaagcgta acttcttcca
     2221 tgctttggaa cccctccctt cgcgggcctg gccctaaccg atcaggtgtt cttctgccga
     2281 aagagaaaag cgtttcggaa cggttccggg tctcgatacg gccgaaaaaa gagtttgcca
     2341 ttccccctca ccccgcccgg agggtcggga aggtcggggg tgggatcggt tccgatctgt
     2401 cgatccccat gatgataaac gtcgccgacc ccctgggaag gcgagctcta ttgtagtcgg
     2461 gatccggagt agagccgggg tcgatctctt ctttccagga ggtgttggag cccagcaggg
     2521 tgacggtctc tcgccggaag tcgaagtcga ccggcgtgcc gttcggggtc gtgagggtgt
     2581 acggggcggt aagatcgatc gccaggtaga ggacctcttc ctggccgggt ctcgggaggt
     2641 ccgccgtcac ccgcacctcg cgccactctc cggcggcgag gtcgtcgata ggcagcgtgg
     2701 tcgcggcgcc gggggggatg ttcccctccg gggatccggt ggtggtgttc agggttgtgg
     2761 tccccgtcgt catggcaagg tcggcccggg cacagtcctc gcccgcaagg ttccggatga
     2821 gaaagtcgag gctgacctga ccgccctcga ttgcgacatc aggggtgtac cggatatctt
     2881 ccgggtgggc cgggtcgaga ctgtagcggt cgccgaggtt aattgtccgg ttgtaggcag
     2941 taacgttccg caggtgccag gtctcgttcg ctccgtcgta ggcgatcacc gggctctcgt
     3001 aagggccgcg gtactcgaac cccggcccga acggcagtat ccagtccacc cgcttgacgg
     3061 gttcctgcac atgctgctgt cccgggggga aagcggcgag tatggcgagg ttggccgcgg
     3121 caaggaggag aacaacaccg ataccgagcc atatccacgt ttttgggtgc attgtatcac
     3181 gccggagggt tcagaaccat atctgaatcg gtatgcggtc gtataaaacc cactgtggtt
     3241 gttcggttct aaccggataa acctttagcg ttacgattta cgtgcggcag cagaaagatg
     3301 agagtttccc ggattgtcga ccggatacaa aggtttatgg tgtgttcggc tggagtcgaa
     3361 cggtgggtgc cggatttata tgatctgaag gctcagaatc gtgttgggtg ccgtgtgtgg
     3421 aaccgggcac acgcgatcca cccggggggg tgaatacccg gaggagagca gatggacgaa
     3481 agttcggtca ttcttgagga agatcagtga ctccgttgca ccacgagaag agcgcccggt
     3541 tgaggcgata cctcatcgcg gtcgcggccg ccgggttcgt cctgctcggt tgctacgtcg
     3601 tcgtttcctc ctacatcgcc gcgaccattc cacccgacgc attcctgctc tacgaggtca
     3661 cggaggagac aactcagaag ggtatcgtca ttcgcctcga tgaggaggag ttccggaatt
     3721 tgccggttct ggatgcgttc ctccgcgatg aggagcataa caccgaggga gagccgtatg
     3781 ctcccgggga catccggttg gtggggagcg tagattaccc ggagaagatc cgccaggaaa
     3841 cgatcgacgc ctacatcttc gatagcgaga ccggcaggcg gaagtacttc gaatacgagg
     3901 gacggtacta ccgggtcgga acgatttacc gggactgagc ggttggagga gaaacggggg
     3961 gcgtttagag gttttaccgg ataatcccgg tttccagcgc gttaagtccg cttcccgacc
     4021 cgaaaccaat tcagaagccc caacg
//
LOCUS       DAOSII010000163         4025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_19705_length_4025_cov_47.475063, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000163 DAOSII010000000
VERSION     DAOSII010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19705_length_4025_cov_47.475063"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1140..1212)
                     /locus_tag="PLV90_09430"
     tRNA            complement(1140..1212)
                     /locus_tag="PLV90_09430"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1177..1179),aa:Asn,seq:gtt)
     gene            complement(1252..1551)
                     /locus_tag="PLV90_09435"
     CDS             complement(1252..1551)
                     /locus_tag="PLV90_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97374.1"
                     /translation="MLKKLKSEIELASRHLEVIRAVVEHQPIGIMKLSEILDLPYHRV
                     RYSLRILEQEGYIRASPAGAVATALAADLLSGLDAEIGELIGLLVTVQEENSRNL"
     gene            complement(1585..1821)
                     /locus_tag="PLV90_09440"
     CDS             complement(1585..1821)
                     /locus_tag="PLV90_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97375.1"
                     /translation="MMDEATKQAFKGRFVMLTVMLNIVILCFAMAAFVLLRFAPEGAP
                     GLVIGVILLAIGAAFSVSFWKRYTLTKAWLHEQP"
     gene            1878..3008
                     /locus_tag="PLV90_09445"
     CDS             1878..3008
                     /locus_tag="PLV90_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOF97376.1"
                     /translation="MNGLDSVIRDAGAAAYAAYGSSADANVRYLTRFRTTDPVIYVAK
                     PGERGMIVVPQMEHERAVRESIAAVATRADAGYLEYIKAGESRWRATAHVIADLAGGP
                     VLVPATFPLALARELETFHPVVLDGAGSVEGMRAVKTPEEIERIRSVQRAAEAAIDRG
                     VTLIRSSAPRAGVLCRDGAPLTSEAVRTAMHTVLLAHGCRGVDTIVSCGPETALPHNT
                     GSGPLLENEPIVIDIFPRDELSGYYADMTRTVVKGEPSPEIREMYDAVRDAKTLAAAE
                     VRAGAAGADLYNAVVEFFRDRGYESDTRGFIHSLGHGVGLEVHEEPSLGPRGGALAVG
                     NVVTIEPGLYYPGIGGVRLEDMGAVTETDFDRFTQYEEELTL"
     gene            3005..3886
                     /gene="map"
                     /locus_tag="PLV90_09450"
     CDS             3005..3886
                     /gene="map"
                     /locus_tag="PLV90_09450"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II methionyl aminopeptidase"
                     /protein_id="HOF97377.1"
                     /translation="MIMDDVVYDAYREAGALARKVLHQGAGLVKEGAGLLEMVEATEA
                     MVMEEGALLAFPLNVSFNEAAAHDTAMPGDERVFSRGDLIKVDLGVLIDGYIADTAMT
                     VDLGDHAPLVEASAGALEAAIAAVRPGVAAGELGAIIQATIEERGYRPVANLTGHGLD
                     RYNLHARPTIPNVAMSGGAVIEEGMVFAIEPFATTGSGRISEAARVEIYRQIAARPAR
                     LPSAKRILEVARPRRGLPFARRWVPGDKVEIGLATLVRTGILHPFPVLHDIPDSFVSQ
                     AEHTLIVTADGCEVTTR"
BASE COUNT          752 a         1289 c         1289 g          695 t
ORIGIN      
        1 gtatcgatca gtacgagctt cggcacatcg atcacccatc gatgcatggg gttggatctg
       61 cagggttagg aaggtttgct tccgtgttga tgcccgccag tgctgcacga gccctctatg
      121 gggtacctgc gggatgctgc gtcttctggt tgaccctccc tgttaagggg catgtctctc
      181 ccggatacat cggggaggat gtcctgggtc tgtgagggta tcttctggaa ccaggggaaa
      241 aatttatccc ctggaacctc ctttccacct tcgggtgatc tatcattcct gaatcgatct
      301 attgtgaaca ctgtgggcaa gaattctcgc cgaaacagga agacgcgcgc ggcaacgtgc
      361 gattttgcag cgctgagtgc caggtggcag caggggagca ggaccccggc cggaggggac
      421 gccactcgga gaagttcctg gtctccggtc agtcaccctg gagcaggtaa cccggcacta
      481 cgagcgatct ctcttcatga ggtcgggtgt ggcggtgcaa agggcatgca ctgctggaga
      541 gtgggaatcc cggtacgcct gccgggagcc tccgggaaac gcccctctcc ccgttcgatc
      601 cccacgtcgg cactgcctcc cgcgagataa accggtgatg caggtgatcg ccccttcctg
      661 gcccgggtgc tgatcccggc gccagaccgg gcgggacgac gacccccggc agcccgttca
      721 ccctttccgg ccggatcgag gccgggttcg ctgcggcacg cgcgaagacc gggacgatgc
      781 ggcagtccat cctcgcccgg gcattctccg gcactctggt ccccgccggg gcggaactcg
      841 cccggcgaga agggcggggg tacgggccgg cggcggttct ccttaggcag acacgcctgg
      901 agaagaaagg ggggaggaga gggggccggc cgggcactca gtagcgagta tcggggtacg
      961 ggcattcctc tcatgctttt cttgggtatg ggggagcgag agggataccc ggcattctct
     1021 ggattgaatg ttcggagtta tgcaatacaa cgacaggact cgaacctgtg acatgctggc
     1081 aaacaaccag cctgtctact gaataagaaa ccagaggaga taaccggaaa ataacagagc
     1141 gccgccaaca ggactcgaac ctgtgacatg ctggttaaca gccagccact ctaccaactg
     1201 agttatggcg gcactggaat aagtgctcta taatgtctcg tctgaaccgt attaaagatt
     1261 acgggaattc tcctcctgga cggtcacgag aaggccgatc agttcgccga tctcggcatc
     1321 gaggccggaa aggaggtcgg ccgcgagcgc ggtcgccacg gcgcccgccg gcgatgcccg
     1381 gatatatcct tcctgctcga ggatccgcag cgaatagcgg acccggtggt aggggaggtc
     1441 gaggatctcg gagagcttca tgatgccgat gggctggtgc tccacgaccg cccgtatcac
     1501 ctcaaggtgc cgtgaggcaa gttcgatctc agatttcaac ttcttcagca tatccgcctg
     1561 ccgatgtacc atcattcgcg catgttacgg ctgctcgtgc agccaggcct tcgtcagggt
     1621 gtagcgcttc cagaacgata cggagaacgc cgccccgatg gcgaggagga taaccccgat
     1681 cacgagaccc ggggccccct cgggtgcaaa ccggaggagg acaaaggcgg ccatggcgaa
     1741 acagaggatg acgatgttca gcatgaccgt cagcatgacg aacctgccct tgaaggcctg
     1801 tttggttgcc tcatccatca ttcatacttc aacgcggggg taaaagtact tgtggtttca
     1861 acgaaagagg tgggagaatg aatggtctgg attctgttat ccgggatgcc ggggcggcag
     1921 catacgcggc ttacggctcg tcggcggatg ccaacgtgcg ctacctgacc cggttccgca
     1981 cgaccgaccc cgtgatctac gtggcaaagc ccggcgagcg ggggatgatc gtcgtccccc
     2041 agatggagca cgagcgagcg gtccgggaat cgatcgccgc ggtcgccacc cgcgccgatg
     2101 ccggttacct cgagtacatc aaggccgggg aatcgcgctg gcgggcgacc gcccacgtga
     2161 tcgcggacct tgccggcggc cccgtcctgg tcccggcgac cttcccgctc gccctcgccc
     2221 gggaactcga gacgttccat ccggtcgtcc tcgacggtgc cggctccgtc gaggggatgc
     2281 gagcggtcaa gacaccggag gagatcgagc gcatccgctc cgtccagcgg gccgccgagg
     2341 ccgctataga ccggggagtc acgctcatcc gctcctccgc tccccgggct ggcgtcctct
     2401 gccgggatgg cgcccccctc acgtctgagg ccgtccgcac ggcgatgcat accgtcctcc
     2461 tcgcccacgg gtgccgggga gtcgacacca tcgtctcctg cggccccgag accgccctcc
     2521 cgcacaacac aggctccggc ccgctccttg agaacgaacc gatcgtcatc gacatcttcc
     2581 cccgggacga actctccggc tactacgccg acatgacccg gacggtcgtg aagggcgaac
     2641 cctcgcccga gatccgggag atgtacgacg ccgtccgcga cgctaaaacc ctcgccgcag
     2701 cagaagtccg ggccggggcc gccggcgcgg acctctacaa cgcggtggtc gagttcttcc
     2761 gcgaccgcgg ctacgagagc gatacgcgcg ggttcatcca cagcctcggc cacggcgtcg
     2821 gccttgaggt gcacgaagag ccgtcgctcg ggccccgggg aggagcgctc gctgtcggaa
     2881 acgtcgtcac catcgagccc ggactctact acccgggcat cggcggcgtc cgcctggagg
     2941 atatgggcgc ggtgacggag accgacttcg accggttcac ccaatacgaa gaggaactca
     3001 ccttatgatc atggatgatg ttgtctacga cgcctaccgc gaagccgggg cgctggcacg
     3061 aaaggtgctc caccagggcg cggggctcgt gaaggagggg gcaggcctcc ttgagatggt
     3121 tgaagccaca gaggctatgg tgatggagga gggggcgctc cttgccttcc ccctcaacgt
     3181 ctccttcaac gaggccgcgg cccacgatac cgcgatgccc ggtgacgagc gggtcttctc
     3241 gcggggcgac ctcatcaagg tcgaccttgg tgtcctgata gacggctaca tcgccgacac
     3301 cgccatgacc gtcgacctcg gcgaccacgc gcccctcgtc gaggcctccg cgggagccct
     3361 cgaggcggcg atcgctgccg tccgtccggg ggtcgccgcc ggggagctcg gggcgatcat
     3421 ccaggcgacg atcgaggagc gcggctacag accggtggcg aacctcaccg ggcacggcct
     3481 cgaccgctac aacctccatg ccaggcccac cataccgaac gtcgcgatga gcggcggggc
     3541 cgtcatcgag gaagggatgg tctttgccat cgagcccttc gcgaccaccg gatcggggag
     3601 gatcagcgag gcggcgcgcg tggagatcta ccggcagatc gcagcccgtc ccgcacgcct
     3661 gccgtcggca aagaggatcc ttgaagtggc acggccgcgg cgcggcctcc ccttcgcccg
     3721 ccgctgggtc ccgggcgaca aagtcgagat agggcttgcg acgctcgtac gcaccggaat
     3781 ccttcacccc ttccctgtgc tgcacgatat cccggactcc ttcgtctcgc aggcggaaca
     3841 taccctgatc gtcacggccg acggatgcga ggtcaccacc cggtgagtta tgcttattga
     3901 taccgcgcgc caatatgtct gctcatagac atgaccaccg aaacgggtac gcgtgatgtc
     3961 ctcgtcgtca tggaactcct cctcaccgcg gagatattca accggaacga ggctctcgac
     4021 atcaa
//
LOCUS       DAOSII010000164         4020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_19733_length_4020_cov_51.800757, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000164 DAOSII010000000
VERSION     DAOSII010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19733_length_4020_cov_51.800757"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PLV90_09455"
     CDS             <1..205
                     /locus_tag="PLV90_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOF97378.1"
                     /translation="VLPEDIWCTAWTVAPEGFHPRYSAVSRTYRYYFFPAPEEIAAMH
                     EAVQELVGLHDFSTFSRAGNKNP"
     gene            212..562
                     /locus_tag="PLV90_09460"
     CDS             212..562
                     /locus_tag="PLV90_09460"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97379.1"
                     /translation="MLAARVFEEGPFAVFEVTGESFLWNMVRCMATALKRVGNGKIDR
                     GAIALLLACPAGEGLPAAPPEGLILWDVEYGFPFTPLPADERSRRYPVGRHRYHMLMA
                     ELSARLVPGEGPPG"
     gene            1118..2185
                     /locus_tag="PLV90_09465"
     CDS             1118..2185
                     /locus_tag="PLV90_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOF97380.1"
                     /translation="MDTETDRWIERHVPQKIGEGSPMDIPALRCEVQEQVNPLRMPTG
                     PGHRRVAGEVAGPAAAPSRRSARVRTLAAIPCFNEEIAIGSVVLMARLHADEVLVIDD
                     GCTDGTATVARDAGATVISHGAQMGKGRGIRSALRYAVDHDYGCLVFMDGDGQHNPGE
                     IPLLVEPILTDTADLVIGFRTFDQMPFYRRFGRAVLDVASSNGSSITDSQCGFRALNR
                     TAIDAMLDGLKKDDFSTESEMLRTARENCLRVGEAPINCKYGGDLSTSTKNPISHAIE
                     VLGSIFWLAVERKPLLHIGLPGLAAVLAGIFLLLQFFRGFGETGLVAIEQGMLASLFL
                     IPGTVALIVGLMLALIARLRE"
     gene            2223..3302
                     /gene="wecB"
                     /locus_tag="PLV90_09470"
     CDS             2223..3302
                     /gene="wecB"
                     /locus_tag="PLV90_09470"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOF97381.1"
                     /translation="MIGIILGTRPEIIKMSPVIRECERRGLDYFILHTGQHYSYEMDR
                     LFFEELELPDPDYNLDVGSGSQAGQTAKIMTGVEDVLVKEAPDLILVQGDTNTVMAGA
                     LAASKLHVRVGHIEAGLRSFNRRMPEEINRILTDHMSDYLFAPTENARKNLLAEGIAD
                     RRICVTGNTVVDAVYQNLDISRKKVSVLKDLDLKSREYFLVTAHRQENVDNRARLKEI
                     LRGLLGVQKEFALPVVFPVHPRTEKRIKELGIGIDGLNFTKPFGFLEFLQLESQAKAV
                     LTDSGGVQEETCVLGVPCATMRYDTERPETLNVGSNVLVGADSRKILEGVRFAVTRKP
                     GWENPYGDGIAGKMIVMVCVAAQSQ"
     gene            3323..>4020
                     /locus_tag="PLV90_09475"
     CDS             3323..>4020
                     /locus_tag="PLV90_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOF97382.1"
                     /translation="MKICVLGLGHIGLPTALLFAAHGADVVGVDVKQSVVDCLNHGSL
                     PFREPGLEDLYREAEERFAARARPGTADVFLIAVPTPLDQATKVSDLSYVKKAADSIA
                     PHLARGNLVILESTVPPGTSERVVIPRLEKKGIPIGDFLYAHCPERAIPGRTLQEMTE
                     NSRIIGGYDRDSTDRATSIYQTFVRGQIYQTDTRTAEFVKLMENTCRDVNIALANEFA
                     QLAEECGINVWEAIT"
BASE COUNT          842 a         1163 c         1255 g          760 t
ORIGIN      
        1 ggtgctcccg gaggatatct ggtgtacggc gtggactgtg gcgccggagg ggtttcaccc
       61 ccgctacagc gccgtatcga gaacttatcg gtattatttc ttcccggcgc ccgaagagat
      121 cgccgccatg cacgaagcgg tgcaggagct cgtcgggctg cacgacttct cgacgttctc
      181 ccgcgccggc aacaaaaacc cctagcgtcg gatccttgcg gcacgggtct ttgaggaggg
      241 tccgtttgcc gtctttgagg tgaccggaga gagtttcctc tggaatatgg tccgatgcat
      301 ggcaacggca ctcaaacggg tggggaatgg caagatcgac cggggagcta tcgccttgct
      361 tcttgcgtgt ccggcgggcg aggggctccc ggccgccccc ccggaggggt tgatcctctg
      421 ggatgtcgag tacgggttcc cctttacgcc tctccctgct gatgagcgga gccgccggta
      481 tccggtcggc cgtcatcgct accacatgct catggcggag ctctccgcac gccttgtgcc
      541 gggagaaggt ccgccggggt gatctgtgcc ttcgccggtc tcgtgcggca gccggcacac
      601 accataaagg taccgtatcc ggcaaaaacg gagaaacgac aaccgagaaa cgggtccttt
      661 tcatcaccgc tggtaaaaaa ggatcctgcc gcagacagcg gatctcccaa aggaggtgcg
      721 gcagggtaaa tccggctttt tgcctgccat cggtgaaggc cgggcttaaa cttttcggat
      781 agcaccttac acgataaccc cggaccgctg gaatcgccgg atgaggatct gcaaccgtcg
      841 accgatatgg gatcctgagt cacggcacgg aggcaggaac gcttaattag tccggtggag
      901 acttaagagt actcctgtaa gctgatttca gcttactttt ccaatatgtt aagcaataaa
      961 ccttaataat aacctattaa tatatgccag cacagtcccg cttcagtacg tcaagagggg
     1021 aaagtggatg tacgacaagg atctggttcg ggcgagacca cccgctcacc acgaggggtg
     1081 gttcgccgaa gagacagctg tgccgttacc gcagttcatg gatacagaga cagacaggtg
     1141 gatcgaacga catgtgcccc agaagatcgg agaggggagc ccgatggata ttcccgctct
     1201 ccgttgcgag gtccaggagc aggtcaaccc gctgaggatg ccgacgggcc ccggacaccg
     1261 gcgggtggca ggcgaggtcg ccgggcccgc agccgcccca tcccgtcggt cagccagggt
     1321 gaggaccctc gcggcgatcc cctgtttcaa cgaagagatc gccatcgggt cggtcgtcct
     1381 gatggcccgg ctgcacgccg acgaggtcct ggtgatcgat gacggatgca cggacggcac
     1441 cgcaacggtc gcccgggatg ccggtgcgac cgtcatatcg cacggagcgc agatggggaa
     1501 ggggcggggg atcaggagcg ctcttcgata cgcggtcgat cacgattatg gctgcctggt
     1561 cttcatggac ggagacggtc aacacaaccc cggggagata cccctcctgg tcgagccgat
     1621 cctcaccgat accgctgatc tcgtcatcgg ttttcggacc ttcgaccaga tgccctttta
     1681 ccggaggttc ggccgggccg tactcgacgt cgcctcaagc aacggcagtt cgatcaccga
     1741 ctcccagtgc gggtttcgtg cgttaaacag gacggccata gatgcgatgc tcgatggact
     1801 gaagaaagat gatttttcga ccgagtcgga gatgctcaga accgcacgag agaactgtct
     1861 gcgcgtcggg gaggcgccga tcaactgtaa atacggcggc gatctcagta cgtccacgaa
     1921 aaatcccata tcccacgcga tagaggtgct cggatcgata ttctggctcg ccgtggagag
     1981 aaaacctctc ctgcatatcg ggcttcccgg acttgccgcg gtcctcgccg gcatctttct
     2041 cctccttcag ttcttccggg ggtttggcga gacgggtctt gtcgcgatcg agcaggggat
     2101 gcttgcatcc ctcttcctga tccccgggac cgtcgctctc atcgtgggtc tgatgcttgc
     2161 tctcattgca cggctgcggg agtgaggggg agaagaggta cgatacagag gaggagcgca
     2221 gaatgatagg aataatactt gggacaaggc cggagatcat caagatgtct ccggtcatca
     2281 gggaatgcga gagaaggggg ctcgactact tcatccttca tacggggcag cactactcct
     2341 acgagatgga ccggctcttc tttgaggaac ttgagctccc ggaccccgac tacaacctcg
     2401 acgtgggttc cgggagccag gccggccaga cggcgaagat catgaccggt gtcgaggatg
     2461 tcctcgtaaa ggaggcgccc gacctcatcc tggtgcaggg ggatacgaac accgtcatgg
     2521 ccggggcgct tgccgcatcg aaacttcacg tgcgggtggg gcacatcgag gcgggcctcc
     2581 ggagtttcaa ccgccggatg cctgaggaga tcaaccggat ccttaccgac catatgtcgg
     2641 attacctctt tgccccgacg gagaacgccc ggaagaacct ccttgcagag ggtattgcag
     2701 acaggaggat ctgcgtgacc ggcaacaccg tcgtcgatgc ggtctaccag aacctcgata
     2761 tatcgaggaa gaaggtcagc gtcctcaagg atctcgacct caaatcccgc gaatacttcc
     2821 tcgtcaccgc ccaccggcag gagaacgtgg acaaccgggc acggctcaag gagatcctga
     2881 gagggcttct aggggtacaa aaggagtttg ccctgccggt cgtcttcccc gtccacccga
     2941 ggaccgagaa gaggatcaag gagctcggta tcggcatcga cggattaaac ttcaccaaac
     3001 ccttcgggtt tcttgagttc ctgcagttag agtcgcaggc gaaggccgtg ctgaccgatt
     3061 ccggcggcgt ccaggaggag acctgtgttc tcggcgttcc ctgcgcgacg atgcgctacg
     3121 atacggagcg gcccgagacg ctgaatgtcg gatcgaacgt cctggtcggt gccgactcaa
     3181 ggaagatcct tgaaggagta cgctttgcag tcacccggaa acctggctgg gagaacccct
     3241 atggggacgg tatcgccgga aagatgatcg tcatggtctg tgtagcagcg cagtcccaat
     3301 aaccgcacga ggagatgatg ttatgaagat ctgtgtactg ggattaggac acatcgggtt
     3361 gccgaccgcg ctgctgtttg ccgcccacgg ggcggatgtc gtgggtgtcg atgtgaaaca
     3421 gagcgtggtg gattgcctga accacggaag cctcccgttc agggaaccgg gactcgaaga
     3481 tctgtaccgt gaggcagaag aacggttcgc cgcaagggcc aggcccggaa ccgcggatgt
     3541 cttcctgatc gccgtgccga cacccctgga tcaggcgaca aaggtctccg acctctccta
     3601 cgtcaagaag gcggccgact cgatcgctcc ccacctcgcc agggggaacc tggtcatcct
     3661 cgaatcgacc gttccacccg gaacgagcga gcgggtcgtc atcccccggc tcgagaagaa
     3721 aggcatccct atcggggatt tcctctacgc tcactgtccg gagcgggcga taccggggcg
     3781 gactctccag gagatgacgg agaacagccg tatcatcggg ggatacgacc gggattccac
     3841 cgaccgggcg acctctatct accagacctt tgtccgcggg cagatctacc agaccgacac
     3901 gaggacggcg gagtttgtca aactgatgga gaacacctgc cgcgacgtga atatcgcgct
     3961 cgcaaacgag ttcgcccagc tcgcggagga gtgcgggatc aacgtctggg aggccatcac
//
LOCUS       DAOSII010000165         4020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_19734_length_4020_cov_43.761665, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000165 DAOSII010000000
VERSION     DAOSII010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19734_length_4020_cov_43.761665"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /gene="cas1"
                     /locus_tag="PLV90_09480"
     CDS             complement(<1..734)
                     /gene="cas1"
                     /locus_tag="PLV90_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HOF97383.1"
                     /translation="MTATEPWLPVFGYGGHIKATTQELIIADGSTIRRYPLQAVKHLL
                     VVGGHTLHTSAVTNLLKAGAAITFFDIDGLPVGYLYPYGYRPDGAVREAQERAPPHRF
                     AQHLATAALQSRLLHLEELYGRAGHEVFYAGELDFLHQAREEVAASVTMEELRRLSRL
                     TGDMYYEILSRMLPPELGFRRRAARPHTDPVNAMFSLGYSMLYGNCCVSLVGAHLDPD
                     RGMLHEGAGSLVHDLIEPQKAVMVDRT"
     gene            1208..>4020
                     /locus_tag="PLV90_09485"
     CDS             1208..>4020
                     /locus_tag="PLV90_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97384.1"
                     /translation="MAEASDAGDMSLTESITSDIIDAAAAPEAAAAFTEDPDEVSVLA
                     IEPVILFNGTVALIDGTFECTAYNSDIAYTVQNRTPLGALQAVAERKGFTYDVTDKKW
                     GDFEVLLLDNIGEYEYIKSGSKWVCYVNGVEKDGYNGHNDGLNVIALVDGDEVIFCYG
                     SSPTPDNASVLIKIVAGMEDSEEPPVEPTSWTVTLKGASIETVDQEYFEDGIACGHAA
                     TYTDDDGNEWAGMPLWYLIGFVDDDKKHGSGAFNEALAAKGYSIKVTSSDGYSINFDS
                     ASAAKNDGIIVANTLNGDPLPEVIGDKNKPCWPLQMIGPAVSSGQKVGSVATIELVGL
                     PESSGDWEITLAGAFNRTVSQDEFDDWVGCHGKSYTDSTDQVWTGMPLWYLVGAVDDV
                     NTGSHWKFNETRAAEGYIVRVTAGDGTTAAFDIADIAKNDSFIVANKMNAAVLAEDGK
                     NWPLKFVGPGLSGKQQVGGIVSIALEGLPGADTGSEWTVSLEGPKITYIFTKEEFEDA
                     EACPHHTATYNDGVSTWTGISLKALCGWVDDDVMHGSGAFDATLARTGYTVIVSSGGA
                     SPYSKEFSSMDILANPEDYVVASKINGTPIGDKNFPLRLVGNGAAGSLSVGNIERIQL
                     VDFQKPTESPSIRVVRYASDGKTVVNETTKTCEWMEENLMVYGAPDGIRLRFQGPTFN
                     PNDLWNPAEDINPDKVDEVVKGTSIRDLCDLVGGAPEGGEIDLIASDGYKATINYTNV
                     YTPLERQGEAIIAWWTERQGYVPDYRDGPRLFYNTPDGVFGADDMRVCLAEAYWHYYW
                     DSGIQYPSAAGVSNKNIATIAIKPGAREDWSLILTGAVTDTISRSYFESGKACAMAGH
                     GATWTDDDGHVWSGMPLWVLCGWVDDANKHDYGTDPFRDDLADAGYNVTVIDYGPDGV
                     KGTGDDFSTTFNSSFVKRNRNIIV"
BASE COUNT          855 a         1163 c         1276 g          726 t
ORIGIN      
        1 gtgcggtcca ccatcaccgc tttctgcggt tcgatgaggt cgtggacgag gctcccggcc
       61 ccttcgtgga gcatgccgcg gtcgggatcg aggtgggcgc cgaccaggga gacgcaacag
      121 ttgccgtaaa gcatcgagta gccaagcgag aacatggcgt tgacggggtc ggtgtgcggg
      181 cgggcggccc tgcgccggaa cccgagctcg ggcgggagca tacgggagag gatctcgtag
      241 tacatatcgc cggtcaggcg cgagagcctc cgcagctcct ccatggtgac cgaagccgcg
      301 acctcctccc tggcctggtg gaggaagtcg agctctccgg cgtagaagac ctcgtgcccg
      361 gcgcggccgt agagctcctc aaggtggaga agccttgact ggagggcagc cgttgcgagg
      421 tgctgcgcaa accggtgggg gggggcgcgt tcctgcgcct cgcgcaccgc tccgtccggg
      481 cggtaaccgt acgggtagag gtagccgacg ggcaagccgt cgatatcgaa gaaggtgatg
      541 gcagcgccgg ctttgaggag gtttgtcacc gccgatgtgt gcagggtatg gccgccgacg
      601 acaaggaggt gcttcaccgc ctggagcggg taacgccgga tggtgctccc gtctgcgatg
      661 atcagctcct gtgtcgttgc cttgatgtgt cccccgtagc cgaagacggg gagccagggt
      721 tccgtcgctg tcattcgatc actctggaga tgaatattgg gctgtttttg gtgatgaagg
      781 catcgattgg gcgggatacg gcctgatcgc tccgggtgcg gaatgggtcg gacccggcgc
      841 tgcaccggga tcttgaggag gccttcacgc gagttaaacc gtcggtttcc cttggatact
      901 actgccggtt ttccgttctg gcgccatatg ggctgcagtt atcgatatcg gccgccggaa
      961 tgcgcgaaat gccgggcgcc gatgataccg gccggggtgt taactgcacc aggttaattc
     1021 aaccagatta actaaatgca aactttatcc atggccataa cgtagtttaa aatgttatcc
     1081 aggtcaacgg actgatggga acgagtttca aaagaaatca ggtgatttga tgggttattt
     1141 agaaaaaaga attcagtatg ttctgttcat actgatggta tgtgtgctgg caatacccgc
     1201 atcggcaatg gcagaagcat ccgatgccgg agatatgagc ctgacggaga gcatcacgag
     1261 cgatattatc gatgctgcag cggcaccgga ggcagccgcg gcatttacgg aggatccgga
     1321 tgaggtatca gtgctggcta tagagcccgt gatactcttt aacggcacgg tcgcactgat
     1381 agacggcacg tttgagtgta cggcctataa ctccgacatc gcctacacgg ttcagaacag
     1441 gaccccgctc ggggccctcc aggcggtagc ggagcgcaag ggtttcacgt acgatgtcac
     1501 cgacaagaaa tggggtgatt ttgaagtcct cctgctcgac aacattggtg aatacgagta
     1561 tataaagagc ggatccaaat gggtctgtta tgtcaacggc gtcgagaaag acggctacaa
     1621 cggccacaat gacggtctca acgtgatagc gcttgttgat ggcgatgagg tcatcttctg
     1681 ttacggcagc agcccgacgc ccgataatgc atcagtgctg atcaagatcg tcgcgggtat
     1741 ggaggactca gaagagccgc cagtggaacc caccagctgg accgtcacgc tcaagggtgc
     1801 gtcgatcgag accgtcgacc aggagtactt cgaggatggg atcgcctgcg ggcatgccgc
     1861 cacctacacc gatgacgacg gtaatgagtg ggcgggcatg cctctctggt acctcatcgg
     1921 attcgtcgac gacgacaaga agcacggttc cggggcgttc aacgaggctc tcgccgcgaa
     1981 gggctactct atcaaggtca catcgagcga cggctactcc atcaactttg atagcgcgag
     2041 cgccgcaaag aacgatggta tcatcgtcgc aaacaccctc aacggcgacc cgctgccaga
     2101 ggtcatcggg gataagaaca aaccctgctg gccgctccag atgatcggcc cggccgtgag
     2161 ctccggtcag aaggtcggca gcgtcgccac gatcgagctc gtcggcctcc ccgaatcatc
     2221 gggagactgg gagatcacgc tcgcgggcgc gttcaaccgg acggtctcgc aggatgagtt
     2281 cgatgactgg gtcggatgcc acggcaagag ctacaccgac tctacagacc aggtctggac
     2341 cgggatgccg ctctggtacc tggtcggcgc cgtcgacgac gtgaataccg gcagccactg
     2401 gaaattcaac gaaacccggg cggccgaggg ctacatcgtg cgggtgaccg ccggcgacgg
     2461 gaccacagcc gcctttgata tcgccgatat cgcaaagaac gattcgttca tcgttgcaaa
     2521 caagatgaac gcagcagtgc tggcagaaga tggtaagaac tggccgctga agttcgtcgg
     2581 tcccggcctc tccgggaagc agcaggtcgg cggcatcgta tcgatcgccc tcgaggggct
     2641 gccgggcgca gataccggat ccgagtggac ggtctctctt gaggggccga agataacgta
     2701 tatcttcacg aaggaggagt tcgaggacgc cgaagcgtgc cctcaccata cggcgaccta
     2761 caacgacggg gtcagcacct ggaccggcat ctcgctgaag gccctctgcg gttgggttga
     2821 cgacgacgtc atgcacggct cgggagcatt cgatgccacc ctcgcccgga ccggctacac
     2881 ggtgatcgtc tcctcgggtg gagcaagccc ctacagcaag gagttctcga gcatggacat
     2941 cctggcaaac ccggaggact acgtcgtcgc aagcaagatc aacggcacgc cgataggcga
     3001 caagaacttc ccgctccgcc tcgtcggtaa tggcgcggcc gggagcctga gcgttggaaa
     3061 catcgagcgg atccagctcg tcgacttcca gaaaccgacg gagtcgccct ccatccgggt
     3121 cgtcaggtat gcttccgacg gcaagacggt cgtcaatgag acgacgaaga cctgcgagtg
     3181 gatggaggag aacctgatgg tctatggtgc tccggacggt atccgcctca ggttccaggg
     3241 ccccacgttc aacccgaacg acctctggaa cccggcggaa gacatcaacc ccgacaaggt
     3301 cgacgaggtc gtgaagggaa cctcgatcag ggacctctgc gacctcgtgg gcggagcccc
     3361 cgaaggcggc gagatcgacc tcatcgcatc cgacggttac aaggctacaa tcaactacac
     3421 caacgtctac accccgctcg agcggcaggg agaggcgatc atcgcctggt ggacagagcg
     3481 gcagggctac gtgcccgact accgtgacgg cccgcgcctc ttctacaaca ctcccgacgg
     3541 cgtcttcggc gccgacgata tgcgggtgtg ccttgctgaa gcatactggc actactactg
     3601 ggactccggc atccagtacc cctcggcagc cggcgtatcc aacaagaaca tcgcgaccat
     3661 cgcgatcaag ccgggcgcac gggaggactg gagcctcatc ctgaccggcg ccgtcaccga
     3721 tacgatcagc cggtcctact ttgagtcggg caaggcctgt gccatggccg ggcacggcgc
     3781 aacctggacc gacgacgacg gtcatgtctg gtcgggcatg ccgctctggg tcctctgcgg
     3841 ctgggtcgac gatgccaaca aacacgatta cgggacggac cccttccgtg acgatctcgc
     3901 ggatgcgggc tacaacgtca ccgtcatcga ctacggcccc gacggcgtga agggcaccgg
     3961 cgacgacttc tccaccacgt tcaacagttc gtttgtcaag cggaacagaa acataatcgt
//
LOCUS       DAOSII010000166         4014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_19771_length_4014_cov_48.129326, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000166 DAOSII010000000
VERSION     DAOSII010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19771_length_4014_cov_48.129326"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PLV90_09490"
     CDS             complement(<1..367)
                     /locus_tag="PLV90_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1616 domain-containing protein"
                     /protein_id="HOF97385.1"
                     /translation="MDTEDRQPLILEAAGDVPGDLVAIAAVTAITLACVYVPVLNESF
                     LRILFGVAMVLFIPGYALIAALFPARGDLDGIERVALSFGLSIAVVPLIGLALNYTPW
                     GIRLDPILASLTLFTLAMTA"
     gene            complement(379..1563)
                     /locus_tag="PLV90_09495"
     CDS             complement(379..1563)
                     /locus_tag="PLV90_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97386.1"
                     /translation="MKRLGGFLLLLVLLIPAATAATITISPDTIDSGDTVTVTVNDLP
                     DGASFSLGIRGEFAATPGEEFAFSARGITLPFALNGGEVNAYTKGTNWTELAANLPDG
                     GSVILLKYADPNNEVRISQSQNIGSGTISLVTLRGEAAADTIIADFTVMGTKKGPVDG
                     TISFALEGIEQGTATVSVYINGSEALARTITIGGGVPTTPTPTTTSTPAPTTTESGNG
                     DSSGTPGGSSGGSTGPVSTTAPTTATVTSTDGKASLAGTDTAGAGLLAVAAQGTTPAG
                     WTFAGNAYAVTPANRTFDPAVVLSFTLPSAGATATIARLDNGAWSPVPSKVEGDRITA
                     EISRAGSYALLVPAEAPTATTIPATTTAAPVTTTPTAAPLTALLPVIAFATLMLERKR
                     RG"
     gene            complement(1649..2584)
                     /locus_tag="PLV90_09500"
     CDS             complement(1649..2584)
                     /locus_tag="PLV90_09500"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOF97387.1"
                     /translation="MDTRALRAFIRLGRFPFLLSGFIPFTAGALFGFLLGARFTPGEF
                     LFGYAAMAAAHLSVHYSNDYFDADADRFVETTAISGGSGVLAAHPGLKPAALAAAVAL
                     MAASILIGSLFVAIYGYPAIFLAFGIAGNLLGWFYTAPPFALAYNNLGEVANMITFGL
                     LMPGAGYFVATGRLDPAFFAFAIPLALYGLVFIVSVEIPDREGDIAGGKRTLVVRRGR
                     IFGFCLIAAAAALATAAFALFALTGILSPVDFRPVAVFSLVPLAIALRGLSNRCAGRA
                     GALPHAVANIRGYFIFQTLLALYLLITAAGSLLSP"
     gene            complement(2629..3261)
                     /locus_tag="PLV90_09505"
     CDS             complement(2629..3261)
                     /locus_tag="PLV90_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97388.1"
                     /translation="MAYDCGPLDRSIEETLAALRDGLAREYRLYRRPAHRRSPRRTRR
                     LRRIGGWRRAADRLIFEAGRVARETLPRIERDTAHTFPGPDGLIRVLMDPSTKRLFAG
                     ILAGFPEEALPVPARDLACLAAFSDDARALTLIGDVTLRLRGFSGPEILAALSDRWEL
                     HESPVGRPAGKTLSSEKEALARAVLGLIYVQGGADALERAVRDPGCDPAG"
     gene            complement(3316..3579)
                     /locus_tag="PLV90_09510"
     CDS             complement(3316..3579)
                     /locus_tag="PLV90_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HOF97389.1"
                     /translation="MPLLPAAIAAYILVNAVSFAAFRRDKKLAGRAAWRTPERRLLLL
                     AFFGPLGALAAMRVFRHKTQKAKFRLVPLFLCLHIGIVVLLLL"
     gene            complement(3583..4011)
                     /locus_tag="PLV90_09515"
     CDS             complement(3583..4011)
                     /locus_tag="PLV90_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HOF97390.1"
                     /translation="MKHKGCAKNLSRRVEDYLEAILNVTLEKGYARTKDVACELDLSP
                     SSVVEMFRKLDEMGFVEYRRYEGVVLNPAGREVAEVIKARHDTLKEFLRIIHVPEKIA
                     SADACYMEHELHPETIRQIRLLVEVLRADSGVSERIRRGG"
BASE COUNT          721 a         1223 c         1446 g          624 t
ORIGIN      
        1 ccgcggtcat cgcgagggtg aagagggtga gcgaggcaag gatcgggtcg agccggatcc
       61 cccagggggt gtagttcagc gcaagcccta tgagagggac gacggcaata gagagcccaa
      121 acgagagagc cacccgttcg atgccgtcga gatcgccccg tgcgggaaag agcgccgcga
      181 tcagggcgta gccgggtatg aagaggacca tcgcgacgcc aaagaggatc cggaggaagc
      241 tctcgtttaa caccgggacg tagacgcagg caagggttat ggcggtgacg gcggcgatcg
      301 cgacgaggtc gccgggaacg tcgccggcgg cctcgaggat caggggttgt ctatcttctg
      361 tatccatggg cggttctgtc agcctcttct cttccgctca agcatcaagg ttgcgaaagc
      421 gatcaccggg aggagggcgg tcaggggggc tgcggtcggt gtcgtcgtca cgggggcggc
      481 ggtggttgtc gcggggatgg tcgttgcggt cggcgcctcc gcaggcacca ggagggcgta
      541 agaacccgcc cgggagatct ccgccgtgat ccggtcgccc tcgaccttcg agggtaccgg
      601 cgaccaggcg ccgttatcga ggcgggcgat cgtcgccgtc gcgccggccg aggggagggt
      661 gaaggagagg accacggcgg ggtcgaacgt ccggtttgcg ggggtcacgg cgtaggcgtt
      721 cccggcaaag gtccacccgg caggggtcgt cccctgcgcc gcgacggcaa ggaggccggc
      781 gcccgcggta tcggtccccg caagggaggc ctttccgtcg gtcgaggtca ccgtcgcggt
      841 ggtgggggcg gtcgtggata cgggcccggt cgaaccgccg ctgctgccgc ccggtgtgcc
      901 ggaagaatcg ccgttaccgc tctcggtggt ggtcggcgcc ggcgtcgacg tcgtcgtcgg
      961 cgtcggcgtc gtcggcactc ctccgccgat ggtgatcgtc cgcgcgagcg cctcgctgcc
     1021 gttgatgtag acggagaccg tcgcggtccc ctgctcgatc ccttcgagag caaaggagat
     1081 cgtgccgtcg accggcccct tcttcgtgcc catcaccgtg aagtcggcga tgatggtatc
     1141 ggccgcggcc tccccgcgga gggtgacgag gctgatggtt ccgctcccga tgttctggga
     1201 ttgagagatc cggacctcgt tattcggatc ggcatatttg agcaggatga cggagccccc
     1261 gtcggggagg ttcgccgcaa gctcggtcca gttcgtgcct ttcgtgtagg cattcacctc
     1321 accgccgttg agggcgaagg ggagggtgat cccccgtgcc gagaaggcaa actcctcgcc
     1381 gggcgtcgcc gcgaactcgc cgcggatgcc aagcgaaaaa gaggcgccgt cgggaaggtc
     1441 gttcacggtc acggtgaccg tatcgccgga atcgatcgta tcagggctga tcgtgatcgt
     1501 cgccgccgtc gcggccggga tcagcagaac aagcaggagc aggaatccac ccaatctctt
     1561 cacgaataag atctccttgt cgatgaatac gatatagatg attttcgata ctaaaaaggt
     1621 ttatgaggat gtgccgggat ccggcgcatt acggggagag cagggatccg gctgccgtga
     1681 tgaggaggta gagggcgagg agtgtctgga agatgaagta gccccggatg tttgcgaccg
     1741 cgtgggggag ggcgcctgcc cgcccggcac accggttcga gaggccgcga agagcgatcg
     1801 cgagcggaac aagggagaag accgccaccg gccggaagtc cacgggggag aggatgcccg
     1861 tgagcgcaaa gagggcgaag gccgcggtcg cgagggccgc cgcggcggcg atgaggcaaa
     1921 acccgaatat ccgcccccgc cgcacgacga gggtccgctt tcccccggct atgtcgccct
     1981 cccggtcggg gatctcgacg ctcacgatga agacgaggcc gtagagcgcg agcggtatgg
     2041 cgaacgcgaa gaaggcgggg tcaagcctcc ccgtcgcgac gaagtagccc gcgccgggca
     2101 tcaggaggcc gaaggtgatc atgttcgcga cctcgccgag gttgttgtag gcgagcgcga
     2161 acggcggcgc ggtgtagaac cagccgagaa ggttccccgc tatgccgaag gcgaggaaga
     2221 tcgccggata gccgtagatc gccacgaaga gagaaccgat caggatcgag gccgccatca
     2281 gggcgacggc cgcggcaagc gcggcgggtt tcaggccggg gtgggcggcg aggacgccgc
     2341 tgcctcccga tatggccgtc gtctcgacga accggtccgc gtcggcgtcg aagtagtcgt
     2401 tgctgtagtg gacggagagg tgagcggccg ccatcgccgc gtagccgaag aggaactcgc
     2461 ccggggtgaa ccgcgccccg aggaggaacc caaagagcgc cccggcggtg aagggtataa
     2521 aacccgagag gaggaagggg aaccgcccga gccgtatgaa tgcgcggagg gcacgggtat
     2581 ccatggacga ctctcccgct ccgggagggg cgggtccggt atataacgtt atcctgccgg
     2641 atcgcacccg gggtcccgca ccgcacgttc gagggcgtcg gcgcccccct ggacgtatat
     2701 gaggccgagg acggcccggg caagggcctc cttctccgaa gagagcgtct tgccggccgg
     2761 gcggccgacc gggctctcgt gaagctccca ccggtcggag agcgctgcga gaatctccgg
     2821 cccggaaaag cccctcagcc gcagggtgac gtcgcctatg agggtgaggg cccgcgcatc
     2881 gtcggagaac gccgcaaggc aggcaaggtc ccgtgccggg accgggagcg cctcctccgg
     2941 aaatccggcg agtatgccgg caaaaagccg tttcgtggag gggtccatca ggacccggat
     3001 gaggccgtca ggaccgggga aggtgtgagc ggtatcccgc tcgatgcggg ggagggtctc
     3061 gcgcgccacc cggccggcct cgaatatcag gcgatcggcc gccctgcgcc acccccctat
     3121 ccgccggagc ctcctcgttc tccgggggga gcgccggtgg gcagggcggc ggtagaggcg
     3181 gtactcgcgc gcaaggccgt cccggagcgc cgcgagggtc tcctcgatcg agcggtcgag
     3241 cggaccgcag tcgtaggcca tatgatccag ggttggtcaa ccggacgcat ttacctttcc
     3301 ccggcagtca acaacctaca ggaggagcag aacgacgatc ccgatatgca ggcagagaaa
     3361 gagggggaca agccggaact tcgccttctg tgtcttgtgc cggaagacgc gcatcgcggc
     3421 cagagccccg agcggcccga agaacgcgag gagaaggagc ctcctctcgg gtgtccgcca
     3481 ggccgcccgt cccgccagct ttttgtcgcg gcggaaggca gcaaacgaga cggcgttgac
     3541 gagtatgtag gccgcgatcg cggccggcag gaggggcatg ccttatcccc cccgccggat
     3601 acgctcggat accccggaat cggcccgtaa cacttcgacc agcagccgga tctgccgtat
     3661 cgtctccgga tggagctcgt gctccatgta gcaggcgtcg gcgctggcga tcttctccgg
     3721 gacgtggatg atcctcagga actcctttag cgtatcgtga cgtgccttga tgacctcggc
     3781 gacctcgcgc cccgcagggt tcaggaccac cccctcgtag cgccggtact cgacaaagcc
     3841 catctcgtca agttttcgga acatctccac aacgctcgac gggctgaggt ccagctcaca
     3901 cgccacgtcc ttggtgcggg cgtacccctt ctccagcgtg acgttgagga tggcctcgag
     3961 atagtcctcg acccttctgc tgaggttttt cgcacaacct ttgtgtttca tgca
//
LOCUS       DAOSII010000167         3987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_19942_length_3987_cov_56.057223, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000167 DAOSII010000000
VERSION     DAOSII010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19942_length_3987_cov_56.057223"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            433..2502
                     /gene="fdhF"
                     /locus_tag="PLV90_09520"
     CDS             433..2502
                     /gene="fdhF"
                     /locus_tag="PLV90_09520"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HOF97391.1"
                     /translation="MTETNGKLQYVPTTCPYCGVGCSLNLVVNDGKLVGVEANKRSPI
                     NEGKLCPKGATCWEFVQSPDRLTKPLIKKNGKFEEASWDEAYDLIASKFTEINEKYGP
                     KALGFQVSCRTPNEECYIMQKLARVAFKTNNIDNCARICHGPSVAGLSLSFGSGAATN
                     PFEDVLNADVIFMIGANSLEAHPLAGRRVLQAKKAGKTIIVCDPRFTPTARLADHYVR
                     YNPSTNIALINSIMYWIIQENLHDKEFIEKRTSGFDDLKKTVEKYAEVESITGVPTER
                     IKEIARIYANAKNAVIIYCLGITELTTGTDNVRSLGNLSMLTGNVGRPGVGVNPLRGQ
                     NNVQGACDMGAYPNVFSGYQKCEDDATRKRMEELWGVTGLAKEYGVTLTEQITQCGDP
                     IKAMYIFALNPVVSYPDSNHVMRSLEKLDFLVVQDLFMTETAKYADVILPGASFAEKD
                     GTFTSGERRVNRVRKAVEPPGDAKEDWQIFVDLAHKLGLKGFDFNSPEDIWDDMRRVT
                     PSMAGISYARMEKPESVHWPCPNLEHPGTPILHREKFSSADGLGHFFGLEHRPPAEVA
                     DAEYPFTLMTGRLLFHYHTRTQTGRARILDYEVPEAYVQINNEDAARLKIQNGEKIRL
                     KGRRGQAEALARVTDEVAPGVLMMAMHFGGAGSVNQLTNTALDPLSKMPELKHSAVAV
                     EKITGVQ"
     gene            2504..3742
                     /locus_tag="PLV90_09525"
     CDS             2504..3742
                     /locus_tag="PLV90_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOF97392.1"
                     /translation="MVAKGDMVYAWTTNADIAKVAECGGAVTGLLKFALENDIVDGVL
                     AVKKGVDLYDAVPTLITDPADIAQTAGSLHCGTLLLSKLIKKYLDGANDMRLAVTVKG
                     CDAMGIYELAKRNQVNLDNLLLIGVNCGGSVSPVTARKMIREKFGVDPNDVVKEEIDK
                     GQFIIVTKDGQHKGISMDELEEEGFGRRANCRRCKMKVPRQADLACGNWGVIGDKAGK
                     ATFVEVCSEKGANLLDAAVKAGSLKTDAANPKGIEIRGKVENAMLKLGDKWRARYFEE
                     LGEGKERLQKMMEDSSRCTKCYACIENCPICYCVECSTKKPYLVPPGVLPVPFMFHLI
                     RYAHVADSCINCGQCEETCPMEIANSLYMHSLQVGMEKMFGHVPGVNLELPVLALVEE
                     RAERERLFSTGSDQIFNVFK"
BASE COUNT          919 a         1167 c         1194 g          707 t
ORIGIN      
        1 gtatgaaaat cgatgcatcg ccggaagtgc tcgatcatct ccggagagga gcctttcgac
       61 ctgagtctgg cttcgatatc atcccatgta tgattcagtc tcggttgcac ggaagaatgt
      121 ttggttaagg ttgattgtaa acattttgga tcattaaatt aaattaactt gacatcttga
      181 aagcacattc agggcataat aaagcaaaaa tcatttctaa tcgatataca gtcaacgtat
      241 ggtgcaatat atcaaatggt tgagctcatg tcagatcagg atcaaatata ttcgattgta
      301 tacagacaaa tgttaaaagg gtaagtttaa atttatatac tacttagtag agtaaaacaa
      361 ctctgtgtgc atgtccgcct ttcctgacat gcttggcaac cgacgttgaa cacaacaatc
      421 gggagaatgt gaatgacaga aaccaatgga aagttacagt acgttcccac gacctgcccc
      481 tactgtggtg tagggtgcag ccttaacctc gtggtgaacg atggcaaact tgtgggagta
      541 gaagccaaca agaggagccc gatcaacgag ggtaaactct gtcccaaggg agcaacctgc
      601 tgggaattcg tccagagccc cgatcggctg acaaagcccc ttatcaagaa gaacggcaag
      661 ttcgaggagg catcctggga cgaggcctac gacctgatcg cctcgaagtt cacggagatc
      721 aacgagaagt acgggccgaa agcgctcggg ttccaggtct cgtgccggac accgaacgag
      781 gaatgctaca tcatgcagaa actcgcgcgg gtggccttca agaccaacaa catcgacaac
      841 tgcgcgcgca tctgccacgg gccatcggtc gcggggctct cgctctcgtt cggctccggt
      901 gccgcgacga accccttcga ggacgtcctg aacgccgacg tcatcttcat gatcggcgct
      961 aacagccttg aagcgcaccc gcttgcgggc cgccgcgttc tccaggcaaa gaaggccggg
     1021 aagaccatca tcgtctgcga cccgcgcttt acgcccacgg cgcgcctggc cgaccactac
     1081 gtccgctaca acccctcgac gaacatcgcc ctgatcaact cgatcatgta ctggatcatc
     1141 caggagaatc tccacgacaa ggagttcatc gagaagcgga catcgggctt tgacgacctg
     1201 aagaagaccg tcgagaagta cgcggaggtc gagtcgatca ccggcgtccc caccgagagg
     1261 atcaaggaga tcgcgcggat atacgcgaac gcgaagaacg ccgtgatcat ctactgcctc
     1321 ggcatcacgg aactcaccac cggtacggac aacgtccggt cgctcggaaa cctctcgatg
     1381 ctcaccggca acgtggggag accgggagtc ggcgtcaacc cgctccgcgg ccagaacaac
     1441 gtccagggcg cctgcgacat gggtgcctac ccgaacgtct tctccggcta ccagaagtgc
     1501 gaggacgacg ccacccggaa gcggatggag gaactctggg gcgtcaccgg tcttgcgaag
     1561 gagtacggcg tgaccctgac ggagcagatc acccagtgcg gcgacccgat caaggcgatg
     1621 tacatcttcg cgttaaaccc ggtcgtctcc tatcccgact caaaccacgt catgcggtcg
     1681 cttgagaaac tcgacttcct ggtcgtgcag gatctcttca tgaccgaaac ggcgaaatac
     1741 gcagacgtta tcctccccgg cgcctccttt gccgagaagg acgggacgtt cacgagcggc
     1801 gagcggcggg tcaaccgtgt caggaaggcc gtggaaccgc cgggcgatgc caaggaggac
     1861 tggcagatct tcgtcgacct tgcccacaaa ctcgggctca agggattcga cttcaactcg
     1921 cctgaggata tctgggacga catgcgccgg gtcaccccgt cgatggccgg catctcctac
     1981 gcgaggatgg agaagcccga gtctgtccac tggccctgcc cgaacctgga acacccggga
     2041 accccgattc tccaccgcga gaagttctcg tccgctgacg gtcttggcca cttcttcggg
     2101 ctcgaacacc ggccgcccgc ggaagtcgcc gacgccgagt acccgttcac cctgatgacc
     2161 ggacgtctgc tcttccacta ccacacccga acccagaccg gacgggcgag gatcctcgac
     2221 tatgaggtgc ccgaggccta cgtccagatc aacaacgagg atgccgcgcg cctcaagatc
     2281 cagaacggag agaagatccg gctcaaaggc aggcgcggac aggcggaagc ccttgccaga
     2341 gtgaccgatg aggtcgcgcc cggggtgctg atgatggcga tgcacttcgg cggtgccggg
     2401 tctgtaaacc agctgacgaa caccgctctc gatccgctct ccaagatgcc ggagttgaag
     2461 cacagtgcgg ttgccgtcga gaagatcacg ggggtgcagt gagatggtag cgaagggaga
     2521 tatggtatac gcctggacga cgaacgctga tatcgccaaa gtggcggaat gcggcggtgc
     2581 ggtgaccggg ctcctcaagt ttgccctgga aaacgacatc gtcgacggcg ttctggcggt
     2641 gaagaaaggc gtcgacctct acgatgcggt cccgacgctg atcaccgatc cggcagatat
     2701 cgcacagacg gcaggttcgc tccactgcgg gacgctcctg ctctcgaaac tgatcaagaa
     2761 gtacctggac ggcgcaaacg acatgcgcct tgccgtgacg gtgaaaggct gcgatgcgat
     2821 gggcatctac gagcttgcga agcgcaacca ggtcaacctg gacaaccttc tcctgatcgg
     2881 cgtcaactgc ggcggttcgg tcagcccggt gaccgcccgg aagatgatcc gcgagaagtt
     2941 cggggtcgac cccaacgacg ttgtcaagga agagattgac aagggccagt tcatcatcgt
     3001 cacgaaagac ggccagcaca agggcatctc gatggacgaa ctcgaggaag aagggttcgg
     3061 ccgccgggca aactgccgcc gctgcaagat gaaggtcccg cgccaggccg acctcgcctg
     3121 cgggaactgg ggtgtcatcg gtgataaggc cgggaaggcc acgttcgtcg aggtctgctc
     3181 cgagaaaggc gcaaacctcc tcgacgcggc ggtgaaggct ggaagcctca agaccgatgc
     3241 cgcgaacccg aagggtatcg agatccgcgg caaggtcgag aacgcgatgc tgaagctcgg
     3301 cgacaagtgg cgggcacgct acttcgagga gctcggcgaa gggaaggaac gcctccagaa
     3361 gatgatggag gactcgtccc ggtgtaccaa gtgttacgcc tgtatcgaga actgcccgat
     3421 ctgctactgc gtcgagtgca gcacgaagaa gccctacctg gtgccgccgg gcgtgctccc
     3481 cgtgccgttc atgttccacc tgatccgcta cgcccacgtg gcggactcgt gcatcaactg
     3541 cggccagtgc gaggagacct gcccgatgga gatcgcgaac tcgctctaca tgcactccct
     3601 gcaggtgggc atggagaaga tgttcggcca cgtcccgggt gtgaatctgg aactcccggt
     3661 gcttgcgctc gttgaggagc gggctgagcg agagcggctc ttctcgaccg gcagcgacca
     3721 gatcttcaac gtgttcaagt aagacccccc gcgggggaag acccatggag gtggtggcgg
     3781 gcgaagcgat ccgccatcac cgtccggggt gttagtgcgc ccggcctgtc cggcgggcat
     3841 gcggacgacc gtcgaacaac catcttgagt tccagaaaaa cctcgatgcg ctcgtgccgg
     3901 ggcgcaaaag atgatcgggg tctcgcggga tgaaacctcg gaactcctcc cgcaaacccc
     3961 ggcctatttt ctggcagtgc cgtaagg
//
LOCUS       DAOSII010000168         3957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_20140_length_3957_cov_45.608150, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000168 DAOSII010000000
VERSION     DAOSII010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20140_length_3957_cov_45.608150"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..1212
                     /locus_tag="PLV90_09530"
     CDS             103..1212
                     /locus_tag="PLV90_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04013 family B12-binding domain/radical SAM
                     domain-containing protein"
                     /protein_id="HOF97393.1"
                     /translation="MKIYWRHIPQANNSYAALSAACEVHGYNLEAAEGPRPDVTCYSI
                     NSIDERLYRDEIAGADCITVVGGPHASACYREVARYADYVVVGEGEYTLPALLFAIEE
                     GRDPPPGVATAAGYTPARHTVLLDGHPPFSAVKGFIEITRGCPFSCGYCQTPRLFGRC
                     MRHRSVDEISRYAARFRDIRFVTPNALAYGSDGVHIRLDRVERLLRSLDGRVYFGTFP
                     GEVRPECISQQALDLIVETCANTRLHFGAQSGSDRVLRLLHRGHTVEDVVRAVDLCRD
                     NGLVPVVDFIFGLPFESDDDQRATLDLVRMVTRAGRAHIHYFMPLPGTPLKQSRPRPL
                     LPETEKILGKLALDGRITGSWMDHEIRFFRRTPHL"
     gene            1216..1857
                     /locus_tag="PLV90_09535"
     CDS             1216..1857
                     /locus_tag="PLV90_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOF97394.1"
                     /translation="MTDVLLLADLHGNYGKLDAFLGYDYDAVIIAGDITNFGPLEPVD
                     SVLSRFEVPCFAIPGNCDPREIIDVLERSDTICLHNSWIALGKITLAGFGGSNTTPFG
                     TPFELTEEEIDSELTRIVNHMDRNIHNVLLSHAPPYGVLDSVGENHVGSRSIRKHMTR
                     FDLICCAHIHEARGIVEVDGVRVVNPGPASEGYGALIHFGKEPKDIDIELISV"
     gene            complement(1844..2374)
                     /gene="cyaB"
                     /locus_tag="PLV90_09540"
     CDS             complement(1844..2374)
                     /gene="cyaB"
                     /locus_tag="PLV90_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HOF97395.1"
                     /translation="MLEVEAKFAVRDPGEVRARLDRQGVHMARRQQEHDVYYNAPHRD
                     FAKTDEALRVRYDDAGVTVTYKGPKIRVGSAKAREEFNLAVASGETLEAILSRLGFRR
                     AASVSKTREFYEMGDVTVTLDDVEGLGTFIEIEILTESDREDAARRIGVTAKGLGVDG
                     PPIYTSYLEMLVSKQK"
     gene            complement(2417..3868)
                     /locus_tag="PLV90_09545"
     CDS             complement(2417..3868)
                     /locus_tag="PLV90_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOF97396.1"
                     /translation="MDTVQQQAEAVSYAPAPPEEFCGREEERGRLSAIISRAEEQGQA
                     VMISGPSGIGKSSLLSWLAHEVRGRPGGLPVPVLRAEVFDLPGMIFPAFRELVNDLRR
                     YAAPGGEGLREAIRYAGDVLEKYAAPVEPVGLLPKTGEEIVGIFARSPDVGYDRVCRA
                     FGELLREIGREMAGTGRIAAILLDDVHLASHLDRRLLRDTVRDLPPGILLAFTCRRED
                     GSGYIAICEELRDSRVQEVQLAGMGRNEIRELGEKRFDLAIDDAAAGILEETRNDPFG
                     LVACFNALRRQGLAPSAGNIAGVLAGAGEPVALAFATLTGPERVLVEELCILNPPFPA
                     PVVACMLRFTEADMIRGSGVLRKLPGAGYAFVHPLLQEYCRRELPDDARITLNARAAD
                     CFERTMHRLPGRLHALLSLACHSFYAREYGKAADLNLELGLRFSHREDYDTALVLTER
                     AIASAEQIGDDLLLAAARRQDGLIREKIAGSTG"
BASE COUNT          700 a         1332 c         1207 g          718 t
ORIGIN      
        1 atgggtcgtc accttaattg catgcgcggc gagagtacag gatacgtatg ccgtgcaggg
       61 aaacccggca tgtgcatgct ccacgactgg aacacagcag ccatgaagat ctactggcgc
      121 cacatccccc aggcaaacaa ctcctacgcc gcgctctctg cagcgtgcga ggtgcacgga
      181 tacaatctcg aggcggcgga gggccctcgc cccgacgtca cctgctacag cataaactcc
      241 atcgacgaac gcctctaccg cgacgagata gcgggggcgg actgcataac cgtcgtcggg
      301 gggccccacg cctccgcctg ctaccgcgag gtggcgcggt atgccgacta cgtcgtcgtc
      361 ggggagggcg agtacaccct cccggcgctt ctctttgcca tcgaggaggg gcgggacccg
      421 cctcccggcg tcgcgaccgc cgccggctac acgccggccc gccataccgt cctcctcgac
      481 ggccatcctc ccttctcggc ggtgaaaggg ttcatcgaga tcacccgggg atgccccttc
      541 tcctgcggct actgccagac gccccgcctc ttcgggcggt gcatgcgaca ccgttccgtc
      601 gacgagatca gccggtatgc cgcacggttc cgcgacatcc ggttcgtcac cccaaacgcg
      661 ctcgcttacg ggtcggacgg ggtccacatc cgtctcgacc gggtggaacg gctcctccgg
      721 agcctcgacg gccgggtcta cttcggcacc ttcccgggcg aggtgcgccc cgagtgcata
      781 tcgcagcagg ccctcgacct catcgtcgag acctgcgcga acacgcgcct gcactttggc
      841 gcccagtcgg ggagcgaccg tgtgctccgt ctcctgcacc gggggcatac cgtggaggac
      901 gtcgtccgcg ccgtcgatct ctgccgggat aacggactcg tcccggtcgt cgatttcata
      961 ttcgggctgc cgttcgagag cgacgacgac cagcgcgcaa ccctcgatct ggtgcggatg
     1021 gtcacccggg cggggagagc gcacatccac tatttcatgc cgctgcccgg aaccccgctc
     1081 aagcagagcc ggccgcgccc gctcctcccg gaaacggaga agattcttgg caaactggcg
     1141 ctcgacggca ggataaccgg ctcatggatg gaccatgaga taaggttttt tagacgcact
     1201 cctcatctat agtgcatgac ggatgtgcta ctcttagcag atctgcacgg taactacgga
     1261 aagcttgacg cttttctcgg ctacgactac gatgcagtca tcattgcagg cgacatcacc
     1321 aacttcggtc cacttgagcc tgtagattcg gtactctccc gtttcgaagt accttgtttt
     1381 gcaattcccg gtaactgcga cccccgtgag atcatcgacg tgctcgaacg ctcagatacg
     1441 atctgtctac acaactcctg gatcgcgctt ggcaagataa cgcttgcggg ttttgggggt
     1501 tcgaacacga cccccttcgg cacgccgttc gagctgaccg aggaagagat cgacagcgaa
     1561 ctcacccgga tcgtcaacca catggacagg aacatccaca atgtcctgct cagccatgcc
     1621 ccgccgtacg gagtgctgga ctctgtcggt gaaaatcacg tggggagccg gagcatcagg
     1681 aaacacatga cccggttcga cctgatctgc tgtgcccata tccacgaggc acggggtatc
     1741 gtcgaggtcg acggcgtcag ggtcgtcaac cccggacccg cctcggaggg ctacggggct
     1801 ctcatccact tcggaaagga gccgaaagat attgatatcg aacttatttc tgtttagata
     1861 ccagcatctc gagatatgag gtgtagatcg gtggaccgtc tacacccaac ccttttgcag
     1921 taaccccgat ccgccgggcg gcgtcttccc gatcgctctc cgtcaggatc tcgatctcga
     1981 tgaacgtccc gagaccttcg acgtcgtcga gggtcaccgt cacgtccccc atctcgtaga
     2041 actcccgggt cttcgagacg gatgcggccc gccggaaccc gaggcgggag aggatagcct
     2101 caagggtctc gcccgaggcg accgcgaggt tgaactcctc gcgggccttt gcgctcccga
     2161 cccggatctt cggtcccttg taggtcacgg tgaccccggc atcgtcgtac ctgaccctca
     2221 acgcctcgtc ggtctttgca aaatcacggt gaggggcgtt gtagtagacg tcgtgctcct
     2281 gctgccttct tgccatatgc actccctgcc ggtcgagccg ggccctgact tctcccgggt
     2341 ctctgaccgc aaactttgcc tctacttcga gcataccggt cgcctcagat cgtctcttct
     2401 cgatggcgat aaaccctcac cctgtggagc cggcgatctt ctcccggatg aggccgtcct
     2461 gcctgcgtgc ggcggcgagg aggagatcgt ccccgatctg ctctgcggag gcgatcgccc
     2521 gttcggtcag cacgagcgcc gtatcgtagt cttcacggtg ggagaaccgg agaccgagct
     2581 ccaggttcag gtccgccgcc ttcccgtact cccgcgcata gaaggagtgg caggcaagcg
     2641 agaggagggc gtgcagcctg cccgggaggc ggtgcatggt gcgctcgaaa cagtccgccg
     2701 cacgggcgtt gagcgttatc cgcgcatcgt ccgggagctc gcgcctgcag tattcctgca
     2761 ggagtggatg gacgaaggca tacccggcac ccgggagctt ccgaagtacg ccgctccccc
     2821 ggatcatgtc cgcctccgtg aacctcagca tgcaggcgac gaccggcgcc gggaaaggag
     2881 ggtttaagat gcagagttcc tctaccagga ctctctccgg gccggtgagc gtggcaaagg
     2941 cgagcgctac cggctccccg gccccggcga gcacgccggc tatgtttccg gcggagggcg
     3001 cgagaccctg ccggcgcagg gcgttgaaac aggcgacgag gccaaacggg tcgttacggg
     3061 tctcttcgag gatgccggcg gccgcatcgt cgatagcgag atcgaaccgc ttttccccga
     3121 gctcccggat ctcgtttctc cccatgccgg caagctggac ctcctgcacc cgggaatccc
     3181 ggagttcctc gcagatcgct atgtacccgg atccgtcctc cctccggcag gtgaaagcaa
     3241 ggaggatgcc gggcgggagg tcgcgtacgg tgtcccggag gagccggcgg tcaaggtgtg
     3301 aggcgaggtg gacgtcgtcg agcaggatcg ccgcgatgcg gcccgtcccg gccatctctc
     3361 tcccgatttc ccggaggagt tctccgaacg cccggcacac ccggtcgtac ccgacatccg
     3421 gcgaccgggc gaatatgccg acgatctcct ccccggtctt cgggagcagg ccgaccggct
     3481 ccaccggggc ggcatacttc tcgagcacgt cgccggcata ccggatcgcc tcccgcaggc
     3541 cttctccccc gggggcagcg tacctgcgca ggtcgttcac cagttcccgg aacgccggga
     3601 agatcatccc gggaaggtcg aagacctccg ccctgaggac gggcaccggc agcccgccgg
     3661 ggcgcccccg gacctcatgg gcaagccagg agagaaggga gctctttccg atgccggatg
     3721 ggccggatat catcaccgcc tgcccctgct cctctgcccg ggatatgatg gccgagagcc
     3781 gtccgcgctc ctcctccctg ccgcagaact cctccggggg agcgggagca tacgagaccg
     3841 cctccgcctg ctgctgcacc gtatccatga tcccggccac gattgagccg ggtatacttg
     3901 aacgtttggg cggaggcggc cccacctgaa gcatgaatga agcgcgtttt tcctgca
//
LOCUS       DAOSII010000169         3908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_20446_length_3908_cov_52.605502, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000169 DAOSII010000000
VERSION     DAOSII010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20446_length_3908_cov_52.605502"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..2272
                     /locus_tag="PLV90_09550"
     CDS             104..2272
                     /locus_tag="PLV90_09550"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOF97397.1"
                     /translation="MIKRPPLSEADRIWFPALICIAIIGISSAGCIATTTSADPIRIG
                     VLLPGSGPVAVHSLDGLTWAAGAMNRQGDRTIELVYRDTSAGNISAYAEELAGRDDID
                     IVIGPATSTELIEIAPLVTGRGKLLISPSVTGEIDDNSLFWRTCPTDDRQVEAMFRIL
                     SETGVRNVSLIVANAPYGETFARFAPAAAAANRIRITGAVSVDASSDFEEIAMRIGEE
                     EPGTVVAAVYPEEAVRIADALSRAGSTAGLFLTDAGHSPYLLERLGERAEGIQGTALF
                     ADPTTGYAIAYEELFGEAPPSFAAQTYDALLLAVYTAARQEAAPGEPLAASLRWVVSG
                     DGIAKGWDPEEAGEAAAMIRAGAHPAVTGASGPLRFGEHSSQGPLAGYYTRWTVEGGA
                     FCESTPVSSAGGEPGLPGLSYDPAAGEGVRPVVWMVYPMAKGDRSFADSAYRGLFRAQ
                     ESFSFTKRECTYADIPLLDAVFSLQNFTEKPDLVITEGFEFTDASRAWAEANPDIRFV
                     TLEQAEFGLPNACDVMMVPYGASYLAGVMAANITKTGKIGAIAGAPVPVIDPFVRGFT
                     EGAAAYDPSVDVTVRYVGENAEGFGMPERAGALARDLRAEGVDVILMIAGASNIGIVD
                     TARETGDIYLIGEDTDQSYLAPNLVIASVVKQIDAIVYRAVANEINGTFRGGREVRTL
                     EDDGTMLFVSPRFDAYAGVVADWRERAVAAEGEYLKTALL"
     gene            2417..3676
                     /locus_tag="PLV90_09555"
     CDS             2417..3676
                     /locus_tag="PLV90_09555"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOF97398.1"
                     /translation="MRRKVPAIALITLAAVIFACGCTGTTTTEQAEVQENVVGVLLPL
                     TGDFAESGAASRAAVEVAAGDINDYFASIGSDHRVRLVVEDTATDPAVALEKLKALDA
                     QGIRMVIGPGCSAELEAVRAYADAHGILIVSTMSTAPSLAIPGDNIFRLVSPDTYQAD
                     AMAYYLEEEGIAAVVPVWRGDIWGDELRNLTTTAFKAQNGTVYDGVRYTPGQKDYAGT
                     MADLDVQVGKAIAAHGQENVCVYAVTLNEIAPIMTRAAETQNLTGVRWYGCDGNVLLG
                     TLATPGDAARFAAQTNFTGPTFWQQNLAAAHEATFQKIQNRLGRQPDGYSLASYDALW
                     LLTLTGTQTASAETPKLKIALTKVSDTFAGPFCNVVGLNAAGDRSTVHYCFWSIKADG
                     DGYRWVPDAQYDIWVAGTPPVFEAIGA"
BASE COUNT          733 a         1259 c         1246 g          670 t
ORIGIN      
        1 ccgtccggcg ccggagaatc aataacaaaa catatctgac tcaccgccga atacaacgat
       61 cggctgccga tatcgaccac aggcgcccgg acgaaggatt acgatgatca aacgaccccc
      121 gctatcggaa gccgacagga tatggttccc tgcactcatc tgcatcgcca ttatagggat
      181 ctcttctgca ggctgcatcg ctacgacgac atccgccgat cccatccgta tcggcgtcct
      241 tctgcccggg agcgggccgg ttgccgtgca ttcgctggac ggactcacct gggctgcggg
      301 tgcgatgaac cggcagggcg accgcacgat cgaactcgtc taccgggaca cgagtgccgg
      361 caacatatcc gcgtacgcag aggaactcgc cgggcgcgac gatatcgata tcgtcatcgg
      421 ccctgcgaca agcacggaac tcatagagat cgcccctctc gttacgggcc ggggtaaact
      481 cctcatatcc ccgagcgtca cgggagagat cgatgacaac agtctcttct ggagaacctg
      541 ccccactgac gaccggcagg tggaggcgat gttcaggatc ctttcggaga ccggcgtccg
      601 gaacgtctct ctcatcgtcg cgaacgcacc ctacggggag acgtttgcac ggttcgcccc
      661 ggcagcagcc gcggcgaacc ggatccggat aaccggcgcg gtatccgtcg atgcatcgag
      721 cgatttcgaa gagatcgcca tgcggatcgg ggaggaggaa cccgggacgg tcgttgccgc
      781 cgtgtacccg gaagaagccg tccggatagc ggatgcactt tcccgggcgg gctcgaccgc
      841 cggcctcttc ctgaccgatg ccggccactc gccctacctg cttgagcgcc tcggcgagcg
      901 ggcggagggc atccagggaa cagcgctctt tgccgacccc accaccgggt atgcaatcgc
      961 ctacgaggag ttgttcggcg aggccccgcc gtcgtttgcc gcacagacct acgatgccct
     1021 cctcctcgcc gtctataccg cggcacggca ggaggcggcc ccgggtgaac cgctcgccgc
     1081 atcgctccgc tgggtcgtct ccggcgacgg catcgccaaa ggctgggatc ccgaagaagc
     1141 aggcgaggcc gcggcgatga tccgtgcggg cgctcacccg gccgtcaccg gcgcctcggg
     1201 ccctctccgg ttcggcgagc actcatccca ggggccgctc gcagggtact atacccgctg
     1261 gactgtcgag ggcggggcgt tctgtgagag cacccccgtc tcttctgcgg gcggagaacc
     1321 ggggctgccc ggcctctcgt acgatccggc cgccggcgag ggcgttcgcc cggtcgtctg
     1381 gatggtctac ccgatggcaa aaggcgatcg gtcgtttgcc gactccgcgt accggggtct
     1441 tttccgtgcg caggagtcgt tctcgttcac gaagagagag tgcacctacg ccgacattcc
     1501 gctcctcgac gccgtgttct ctctacagaa cttcaccgag aagcccgacc tcgtcatcac
     1561 cgaggggttc gagttcaccg acgcctcccg ggcgtgggca gaggcaaacc cggacatccg
     1621 tttcgtcacc ctcgaacagg cggagtttgg tcttccgaac gcatgcgacg tgatgatggt
     1681 cccttacggc gcatcctacc tcgcgggcgt gatggcggcg aatatcacga agacgggaaa
     1741 gattggcgcg atcgccggcg caccggtccc cgtcatcgac ccgttcgtca gggggttcac
     1801 tgaaggtgcg gcggcatacg atccgtcggt agacgtcacg gtgcgttatg tcggcgagaa
     1861 cgccgaaggg ttcggcatgc cggagcgggc gggtgcactc gcccgggacc tccgcgccga
     1921 aggcgtcgac gtcatcctca tgatcgcagg cgcttcaaac atcgggatag tggatactgc
     1981 acgggagacc ggggatatct acctcatcgg cgaggatacc gaccagtcct acctcgcccc
     2041 gaacctcgtt atcgcatcgg tggtgaagca gatcgatgcg atcgtctatc gcgcggtggc
     2101 aaacgagatc aacgggacgt tcaggggagg aagggaggtc cggacgctcg aggacgacgg
     2161 caccatgctc ttcgtctctc cccgcttcga tgcgtatgcg ggggttgtcg cggactggcg
     2221 ggagcgggcc gtcgccgcag agggagagta cctgaagacg gcgctcctat gagcggaacg
     2281 gcgtgctgcc ttccgggcag caccgttctt tctctggcac cctgaacggc aatccggcga
     2341 tgaatcatcc gatattcgca gcactgccgg atgcaaaaac agaaaggcta aatcccactc
     2401 ctgcagaaaa aaccttatga gacgaaaggt accagcaatc gcgctcatca cgcttgccgc
     2461 agtcatcttc gcctgcggat gtaccggaac cacgaccaca gagcaggccg aagtgcagga
     2521 gaacgtcgtc ggcgtcctcc tgccgctcac cggcgacttt gccgagagcg gagcggcgag
     2581 tcgggccgcc gtcgaggtgg ctgccggcga tatcaacgac tacttcgcct cgatcgggtc
     2641 ggaccaccgg gtccggctgg ttgtcgagga tacggcgacc gaccctgcgg ttgcgcttga
     2701 gaagttgaaa gcgctcgacg cgcaggggat caggatggtc atcgggcccg gttgcagcgc
     2761 cgaactcgag gccgtccggg cgtatgccga cgcacacggg atcctgatcg tcagcaccat
     2821 gagcacggcg ccttcgcttg ccattccggg cgacaacatc ttccggctcg tgtcgccgga
     2881 cacctaccag gcggatgcga tggcatacta cctggaggag gagggaatag cagcggtcgt
     2941 gccggtatgg cgcggcgata tatggggcga cgaactccgg aacctgacga cgacggcatt
     3001 caaggcccaa aacggcacgg tttatgacgg ggtcaggtac acgccgggcc agaaggacta
     3061 tgccgggacg atggcggacc tcgacgtgca ggtcgggaag gcgatcgcgg cgcatgggca
     3121 ggagaacgtc tgcgtctacg cagtcaccct caacgagata gcgccgatca tgacgagagc
     3181 ggcggagact cagaacctca ccggggtccg gtggtacggg tgcgacggca acgttctcct
     3241 cggaacgctc gcgacgcccg gcgacgccgc ccggtttgcg gcgcagacga acttcaccgg
     3301 tccgacgttc tggcagcaaa accttgccgc ggcacacgag gccaccttcc agaagatcca
     3361 gaaccggctc gggcggcagc ccgacggtta cagcctggcg tcctacgacg cactctggct
     3421 tctcaccctg accgggacgc agaccgcatc ggcggagaca ccgaagttga agatcgcctt
     3481 aacgaaggtt tcggatacgt tcgccggacc cttctgcaat gtggtcggcc tgaatgctgc
     3541 cggcgaccgg tcgaccgtgc actactgttt ctggtcgatt aaggccgacg gcgacggata
     3601 ccgatgggtg ccggacgccc agtatgacat ctgggtggcg ggcactccgc cggtgttcga
     3661 agcgatcggc gcctgagtgc cggcaccccg atccaccttt tttcagaccg aaccggtgcg
     3721 gttcgtaact cttccatggg gaaccattcg tatgccgtgc gcttcgcctc ctcctcgctt
     3781 ccagcgtaac aggcggcggt ctcgatcatg cacgacttgc cctcgccgcc cgatgcacgg
     3841 aacgcctcga tgacctcctc cggtttctgc tcgaagtgga tgagcgcatc gccgatatcg
     3901 ccggcaac
//
LOCUS       DAOSII010000170         3905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_20462_length_3905_cov_49.848052, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000170 DAOSII010000000
VERSION     DAOSII010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20462_length_3905_cov_49.848052"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="PLV90_09560"
     CDS             <1..1209
                     /locus_tag="PLV90_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HOF97399.1"
                     /translation="LLSAIVGGATAADASAEEQIAVTSVSVNPDTLMRGDTGTVTVEI
                     KNTGETGVAISRAELYPNGISVLNDKTYNSVGTIGPGNTMSFTFTVRADAADGIYYPT
                     FYLDIRNGGSVRYSVPVTVESTEIQVNVVDAPETFPVDGKDTITLSVGNPREGSVNGI
                     TITPSGEGVRNTRTAVFLGALASDEEKNASFEITASRSTELTFDVSYRNGINEHHTIL
                     TVPIEIGERAVAPDMVVNNIEIARGGSAITLTGDVTNAGLKDAYSVKVTVDDPATPTD
                     PYPVYVVGGLEPDDFSSFEVTCNAEGASSIPLVVEYRDQDGRTFTETVTVSLNSASQA
                     SQAGAGSTMPSGMTGGPQGGRGGMGMSFGGGFSQIPAMEILLVVIGGVAVVVGWRKGY
                     FGKIRDRFRK"
     gene            1226..1897
                     /locus_tag="PLV90_09565"
     CDS             1226..1897
                     /locus_tag="PLV90_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF97400.1"
                     /translation="MSSPVIELMDVVRVYPLPSGDVTALSGVSLRIDEGEFVAIMGPS
                     GSGKSTLLNQIGCLDRPTSGDLSLAGKNTREMDDRELTNLRLSSIGYIFQKFNLIPLL
                     SAYENVEYPYIMKHRKNDDTGRVRDLLAMMGIDENLSAHKPNELSGGQQQRVAIARAL
                     VNDPAILLCDEPTGNLDSQTSLQIMEILADLHRRGRTLVMVTHNPETATYADRIITIR
                     DGQIA"
     gene            1894..3108
                     /locus_tag="PLV90_09570"
     CDS             1894..3108
                     /locus_tag="PLV90_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF97401.1"
                     /translation="MIGNDIIFALSVRSVRLHFLRSVLAALGIVIGVVAIASIGMMGA
                     NMTLSVTEQLSDMANKLTVTPYRGGGGGMMGGFGGGGGSSDDDAITEKEFKTIQRIVT
                     KYGTAYTVRSESDKIEVGSDEGRATIYGLDTETMREILTVETGEYPRSTTSVVVGPTL
                     AERLDLKIGSKIKIGDPDKGSTTTVRVVGILEERGMSMDLSTDMAIIGSDKLFVGIYG
                     GEGEYDQVNVVVSDLNDIETVKTAIENELNRKEDTVTVQDSSRMMESITSTVSTMTTF
                     VMAIAGISLLVAAVSIFNVMMMSVNERVREIGILRSIGTQRAEILRMFIYEAAIIGIV
                     GAIIGAIVSIVIGYVIVVGMIGSAEYFFAPGSIVYVPMAMAVGAAICIITGIYPAWRA
                     SNLDPIEALQNE"
     gene            3180..3575
                     /locus_tag="PLV90_09575"
     CDS             3180..3575
                     /locus_tag="PLV90_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97402.1"
                     /translation="MVAKKLMGTCATAMLVVIAAACGHTGTGGESQPAGQPQEDVGAP
                     RSPGSAPERREQPQMDYASAAATFGVAGEEIRAAPGEPGQGRKTVADAAATLGVPEEA
                     FRVSEGTTGSGAPPGVSVGGELRERAGVP"
     gene            3572..>3905
                     /locus_tag="PLV90_09580"
     CDS             3572..>3905
                     /locus_tag="PLV90_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOF97403.1"
                     /translation="MTALSGAAERPDGRPDAREPDQDRGRALTGISGLHLLHLTPAET
                     VVADDPSEPVRELLATISDRIRNPLQVLLARADLMDDEETAGRIREQVRRINDAVKYL
                     EGALSDPYS"
BASE COUNT          833 a         1210 c         1218 g          644 t
ORIGIN      
        1 ctcctctcgg caatcgtggg tggtgccacg gcggccgatg cgtcggcgga ggaacagatt
       61 gcagtcacca gtgtcagcgt caaccccgac acgctcatgc gcggggatac cggcacggtg
      121 acggtcgaga tcaagaacac cggggagaca ggcgttgcca tcagcagggc ggaactctat
      181 cccaacggga tctcggttct caacgacaag acgtacaatt cagtgggcac catcggcccg
      241 gggaacacga tgtcgttcac cttcaccgtg cgggccgatg ccgcggacgg catctactac
      301 ccgaccttct acctggacat ccggaacggg ggaagcgtcc gctactccgt tccggtgacg
      361 gtcgagagca cggagatcca ggtcaacgtc gtcgacgctc cggaaacctt tccggtggac
      421 ggcaaagata ccatcacgct ctccgtcggg aacccccgtg agggcagcgt gaacggcata
      481 accatcaccc cgagcggcga aggcgtcagg aacacccgga cggccgtctt cctcggcgct
      541 ctcgcgtctg atgaggaaaa aaacgcctcg ttcgagatca cggcgtcgcg gtcgaccgag
      601 ctcaccttcg acgtctccta ccggaacggg atcaacgagc accacaccat cctgaccgtc
      661 cccatcgaga tcggtgagcg ggcggttgcg cccgacatgg tggtcaacaa tatcgagatc
      721 gcccggggcg gcagcgcgat cacgctgacc ggggacgtga ccaacgccgg cctcaaagat
      781 gcatactcgg tcaaggtcac cgtcgacgat cccgcaaccc cgactgaccc ctacccggtc
      841 tacgtcgtcg gcgggctcga gccggacgac ttctcgagtt tcgaagtcac ctgcaacgca
      901 gaaggtgcat cctccatccc gctcgtcgtc gaataccgtg accaggacgg cagaaccttc
      961 acggagaccg tgaccgtctc gctcaactcc gcaagccagg cgtcgcaggc cggcgccggc
     1021 agcacgatgc cgtccggtat gaccggcggc ccgcagggtg gaagaggggg catgggcatg
     1081 tcgttcggcg gcgggttctc gcagatcccg gcgatggaga tcctcctcgt cgtcatcggg
     1141 ggcgtggccg tcgtcgtcgg atggcggaag gggtactttg gaaagatccg cgaccggttc
     1201 cgcaagtaat ccgggggaag aacagatgag cagtccggtt attgaactca tggatgtcgt
     1261 cagggtttac cccctcccct caggcgacgt cacggccctc tcgggggtat ccctcaggat
     1321 cgacgaggga gaattcgtcg ctatcatggg gccgtcaggg tcgggaaaat cgaccctctt
     1381 gaaccagatc gggtgcctcg accgtcctac gtcgggcgac ctctctctcg cggggaagaa
     1441 cacccgggag atggacgacc gggaactgac caatctccga ctctcttcca tcggctacat
     1501 cttccagaag ttcaacctca tccccctcct ctctgcctac gaaaacgtcg agtatcccta
     1561 catcatgaag cacagaaaga acgatgacac cggaagggtg cgggatctcc tcgccatgat
     1621 ggggatcgat gaaaacctct cggcacataa gccaaacgaa ctctccggcg gccagcagca
     1681 acgggtcgcg atcgccaggg cgctcgtcaa cgatccggcg atccttctct gcgacgaacc
     1741 gaccggaaac ctggactcgc agacgagcct ccagatcatg gagatccttg cagaccttca
     1801 ccgcaggggc cggacgctcg tcatggtgac ccacaacccc gagaccgcca cgtacgcgga
     1861 caggataatc acgatacggg acgggcagat cgcatgattg gaaacgacat catcttcgct
     1921 ctctccgtac ggagcgtccg gctccacttc ctcagatcgg tcctcgccgc cctcggcatc
     1981 gtcatcggcg tcgtcgccat cgcctcgatc gggatgatgg gagcgaacat gacgctctcg
     2041 gtcacggaac agctctccga catggcaaac aaactcaccg tcaccccata ccgcggtggc
     2101 gggggcggca tgatgggagg gttcggtggt ggaggcggca gctccgacga cgatgcgatc
     2161 acggaaaaag agttcaagac tattcagcgc atcgtcacga agtacgggac cgcgtacacc
     2221 gtcaggtctg aatccgataa gatcgaggtc ggatcggacg aagggagagc aactatctac
     2281 ggtcttgata cggagaccat gcgcgagatc ttgaccgtcg agacgggaga gtaccccagg
     2341 agcacgacct cggtggtggt cgggccgacg cttgccgagc gactcgacct caagataggg
     2401 agcaagatca agatcggcga tcccgacaag gggtccacca cgaccgtccg ggtcgtcggt
     2461 atcctcgaag agcgcggcat gtcgatggac ctctccacgg atatggcgat catcggcagc
     2521 gacaaactct ttgtcgggat ctacggcggc gagggcgagt acgatcaggt caacgtcgtc
     2581 gtgagcgact taaacgacat cgagacggtc aaaacggcga tcgagaacga actcaacagg
     2641 aaggaagata ccgtcaccgt ccaggacagc agccgcatga tggagagcat cacctcgacg
     2701 gtctcgacga tgacgacgtt cgtgatggcg atcgcgggta tctcgcttct cgttgccgca
     2761 gtctcgatct tcaacgtcat gatgatgtcg gtgaacgagc gggtacgtga gatcgggatc
     2821 ctccgctcca tcgggacgca acgggcagag atcctgcgga tgttcatcta cgaggcggcg
     2881 atcatcggga tcgtcggagc aatcatcggt gccattgtaa gcatcgtcat cggttacgtc
     2941 atcgtggtcg gcatgatcgg ctccgccgag tacttcttcg ctcccgggag tatcgtctac
     3001 gtcccgatgg ccatggccgt cggcgccgca atctgcatca ttaccgggat ctacccggca
     3061 tggagggcct caaacctcga cccgatcgag gcgttgcaga acgaatagcg tacaaacagg
     3121 ggaacacctg tttttacccc gggcatccgt cgcaagagaa cagattggaa gagagaagta
     3181 tggtagcaaa gaaactgatg ggcacctgtg caaccgccat gctcgtcgtt attgcagcag
     3241 cgtgcgggca caccggcacg ggcggtgaat cgcagcctgc agggcagccg caggaagacg
     3301 tgggggcgcc ccgttcgcca ggatcggccc ccgaacggcg agagcagccg cagatggact
     3361 acgcctcagc cgcggccacg ttcggggtcg ccggggagga gatccgggcg gcacccggcg
     3421 aaccgggcca gggccggaag accgttgccg atgcggcggc aacgctcggt gtcccggaag
     3481 aggcgttcag agtttccgaa ggaacgacag ggagcggcgc tccccccgga gtctccgtag
     3541 ggggagaact tcgggagcgt gcaggagttc catgacagcg ctcagcggtg ccgcggaaag
     3601 gccggacgga agaccggatg cccgcgagcc cgatcaggat cgtggtcggg ccctcaccgg
     3661 gatctccgga ctgcatctcc tgcatttaac gccggcagag acggtcgttg ccgatgatcc
     3721 gtcagaacct gtccgggaac tcctcgccac catctccgat cgcatcagaa accccctgca
     3781 ggtgctcctg gcccgggcgg acctgatgga cgacgaagag accgccggga ggatccggga
     3841 gcaggtccgc cggatcaacg atgccgtcaa atacctcgag ggcgccttat ccgatccgta
     3901 cagca
//
LOCUS       DAOSII010000171         3820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_21035_length_3820_cov_51.493758, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000171 DAOSII010000000
VERSION     DAOSII010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21035_length_3820_cov_51.493758"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1904)
                     /locus_tag="PLV90_09585"
     CDS             complement(<1..1904)
                     /locus_tag="PLV90_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF460 domain-containing protein"
                     /protein_id="HOF97404.1"
                     /translation="MKVFGIDIIKGSVRSRTRRPVYALARAEDGEITGVEEVTGFRLQ
                     RILAAEEPDILAVDSIQEIAADQHELRTFLQALPPSTKLVQVTGGERTESLGKVAARY
                     NISFNKFDPYAEARTTAQVASLGAGVEVIAFENTTDIVVSRHRSPGRGGWSQNRYARK
                     IHGAVMQKAREIEARLRGAGLDYEKKETKAFGGFSRVLFRVVAPRDRVPVHSSRGADV
                     QVRVAGRELDRIRFEPRSSRPRYLIVGLDPGTTTGIAAVDLDGNLVLLTSSRQMTMSD
                     IVEELYGAGKPLIIASDVREMPYSVEKIRRAFNAIPYTPKQSLSVEAKYDLTARFSYT
                     NDHERDALSAALEAFRSLQNKFRNIVKRVRPGVDLDEVRARVLRGQPLDAVLTDLQGA
                     PAAPREAEPAAAVPERPIEDERVMALDGMVKRLRSYVQELQEDLRERDREVERLQQSL
                     RRARSATERKVRRDAELATKDATIESLREQLRGERRRSRRLKKRLERMQTVAELEVSE
                     DYTPLKVLDSLTRDGVRSLQEEIGIAAGDVLYVPRAHGWGRGVVKDLAGMGVRALVVG
                     GEPPDPHLLRVAREAGLPLLPAGAIEPDIRGRTGAAKTGAVSEALGEWEEGQKEYRRE
                     QEAERVEYLF"
     gene            complement(1901..2758)
                     /locus_tag="PLV90_09590"
     CDS             complement(1901..2758)
                     /locus_tag="PLV90_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HOF97405.1"
                     /translation="MPVPAEYVRSLHAYELRILLALERLMRRYQWVPLEVLRPATGFS
                     ESELAYRLGRLMAMGMVRYEKTPYEGYALVFDGYDTLALHTLTRRGTVQALGCLIGVG
                     KESEVYEAMGLGVVVLKFHRVGQRSFQSARLKRGYMPEAGHCPWLFASSHSAKMEYDA
                     LKTLHPAVSVPLPIDQNRHVVAMSFVPGVNLSRAALAEPEIILDEILDNIREAYRLGI
                     IHGDLSEFNVMVDEKQCWLIDWPQWVEIAHPNAGEILARDIGNILQYFKRKYGIEYAF
                     EEALARVVE"
     gene            2808..3701
                     /locus_tag="PLV90_09595"
     CDS             2808..3701
                     /locus_tag="PLV90_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00269 family protein"
                     /protein_id="HOF97406.1"
                     /translation="MQCSKCRRDAVIYQRYSGLHLCEQHFNRDLEAKAKRAIREHRWI
                     ASGDTVAVALSGGKDSSAVLYFLHRLLHERRDVRLMAITVDEGIGGYRDPARARAIAE
                     RIGVPWVTASFREEYGVTLDEIVARKGTGLSCSYCGVLRRALLNRVAREEGVTKFAYG
                     FTLDDEAQSVLMNVLRGDPYRLTRPVREVAGLVPRIKPFMYIPEREVALYAFFHVEGF
                     DLAGCPYAADALRGDVRGILDAYTYRHPATKYSLVNLGEALRNPEQPPEGGLRVCERC
                     GEPCGEVCRACQVLDEVRRGA"
BASE COUNT          612 a         1264 c         1272 g          672 t
ORIGIN      
        1 ttgaagaggt actcgacccg ctcggcctcc tgctcccgcc ggtactcttt ctgcccctcc
       61 tcccattcgc cgagcgcctc gctgacggcc ccggtctttg ccgccccggt cctgccccgt
      121 atatccggct cgatggcgcc ggccgggaga agggggaggc cggcctcccg ggcgacccgg
      181 agaaggtgcg ggtcgggagg ctccccgccg acgaccagcg cccgcacccc catccccgca
      241 agatccttca ccacgccccg cccccagccg tgcgcccggg ggacgtagag gacgtcgccc
      301 gcggcgatgc cgatctcctc ctggaggctc cgcaccccgt cccgggtgag ggagtcgagc
      361 accttgagcg gggtgtagtc ctcggagacc tcaagctcgg caacggtctg catccgttcg
      421 aggcgtttct tcagccgccg ggacctccgc cgttcgccac ggagctgctc gcgcaggctc
      481 tcgatcgttg cgtccttcgt cgcgagttcc gcatcccgcc tgaccttcct ctcggtcgcg
      541 gagcgtgccc gtcgcaggct ctgttgcagc cgctccacct cgcggtcgcg ctcccggagg
      601 tcttcctgca gttcctggac ataactcctg agccgcttca ccataccgtc gagcgccatc
      661 actcgctcat cctcgatcgg ccgctccggg accgctgccg ccggttcggc ctctcgcgga
      721 gccgccggag cgccctgcag gtccgtgagg accgcatcaa gcggctggcc ccggagcacc
      781 cgcgcccgca cttcatcgag gtcgaccccc ggccgcactc gcttcacgat attgcggaac
      841 ttgttctgga ggctccggaa tgcctccagg gccgctgaga gggcgtcgcg ctcgtggtcg
      901 ttcgtgtagg agaaccgggc ggtcaggtcg tacttcgcct ccacggagag cgactgcttc
      961 ggcgtatagg ggatggcgtt gaacgcccgg cggatcttct cgaccgagta aggcatctcg
     1021 cggacgtcgg aggcgatgat gagcggtttt ccggcgccgt agagttcctc gacgatatcg
     1081 gacatcgtca tctggcggga actggtgagg agaaccaggt tgccgtcgag atcgacggcg
     1141 gcgataccgg tcgtcgtccc cgggtcgagc ccgacgatca ggtagcgggg gcggctggaa
     1201 cgcggttcga accgtatccg gtcgagttct ctcccggcca cccggacctg gacatcggcg
     1261 ccccgggagg agtggaccgg caccctgtcc cggggtgcga cgacccggaa aagaacccgc
     1321 gaaaaaccgc cgaaggcctt cgtctccttc ttctcgtagt cgaggccggc acccctgagc
     1381 cgggcctcga tctcgcgggc cttctgcatc acggcaccgt ggatcttcct tgcataccgg
     1441 ttctggctcc acccgccccg cccgggcgag cggtgccggc tcaccacgat gtcggtcgtg
     1501 ttctcaaacg ctatcacctc gacgcccgcc ccgagagagg ccacctgtgc ggtggtccgg
     1561 gcctcggcgt aggggtcgaa tttattaaaa ctgatgttgt agcgtgctgc cacctttccg
     1621 aggctctccg tcctctcgcc gccggtcacc tggacgagtt ttgtcgaggg cgggagcgcc
     1681 tgcaggaagg tgcggagctc gtgctggtcg gcggcgatct cctggatgct gtccaccgcg
     1741 aggatgtcgg gttcctcggc ggcaaggata cgctggagcc ggaaaccggt gacctcctcg
     1801 acgcccgtaa tctcgccgtc ctctgcccgg gcaagggcgt agacgggccg gcgcgtgcgg
     1861 gaacggaccg aacctttgat gatgtcgata ccaaagacct tcactccacc acccgtgcca
     1921 gtgcttcttc gaaggcgtac tcgatgccgt atttccgttt gaagtactgg aggatgttcc
     1981 cgatatcccg tgcgaggatc tcgccggcgt tcgggtgcgc tatctctacc cactgcggcc
     2041 agtcgatcag ccagcactgc ttctcgtcga ccatgacgtt gaactcgctt aagtcgccgt
     2101 ggataatgcc gaggcggtag gcctcgcgga tgttatcgag gatctcgtcg aggatgatct
     2161 ccggttccgc aagtgccgct cgcgagaggt tgacgccggg gacgaacgac atcgccacga
     2221 cgtgccgatt ctggtcgata gggagcggca ccgagaccgc ggggtggagg gtcttcaagg
     2281 catcgtactc catctttgcc gagtggctcg aagcaaagag ccaggggcag tgcccggcct
     2341 ccggcatgta gccgcgcttc aagcgggcgg actgaaacga cctctgcccg acccggtgga
     2401 acttcagcac gacgacgcca aggcccatcg cctcgtaaac ctccgactct ttcccgacgc
     2461 cgatgaggca gccgagtgcc tggacggtcc cgcggcgggt gagggtgtgg agggccaggg
     2521 tgtcgtagcc gtcaaagacg agagcgtagc cttcgtaggg cgtcttctcg tacctgacca
     2581 tgcccatggc catcagcctg cccagccggt aggcgagctc ggactcggag aaccccgttg
     2641 ccggcctgag cacctcgagc ggcacccact ggtagcggcg catgagacgt tcgagggcga
     2701 gcaggatccg gagttcgtag gcgtgcaggg atcgcacgta ttctgcagga acaggcatct
     2761 tctacaccat ttatgtgcca cggtgataaa agaatgatcg aggaaagatg cagtgcagca
     2821 agtgccgccg cgacgcggtc atataccagc gatactcggg gctgcacctc tgcgagcagc
     2881 acttcaaccg cgatctcgag gcgaaggcaa aacgggccat ccgggaacac cgctggatcg
     2941 cgtcggggga tacggtggcg gtggcgctct cggggggaaa ggactcaagc gccgtcctct
     3001 acttcctcca ccggctcctc cacgagcgcc gcgatgtccg gctgatggcg ataaccgtgg
     3061 atgaggggat agggggctac cgcgatccgg cccgggcccg ggcgatcgcc gaacgcatcg
     3121 gcgttccctg ggtgacggcg tcattccgtg aggagtatgg ggtcacgctc gatgagattg
     3181 tggcacgaaa ggggacgggg ctctcctgct cctactgcgg cgtgctccgg cgggcgctgt
     3241 tgaaccgggt cgcccgcgag gaaggcgtca cgaagttcgc ctacgggttc accctggacg
     3301 acgaggcgca gtcggtgttg atgaacgtcc tccggggcga tccctaccgg ctcacccgcc
     3361 cggtgcggga ggtggcaggg ttggtccccc ggatcaagcc gttcatgtat ataccggaac
     3421 gcgaggtggc gctctacgcc ttcttccacg tcgaaggatt tgaccttgcg ggctgcccct
     3481 acgcggcaga tgccctccgg ggggacgtcc gggggatcct ggatgcctac acctaccgcc
     3541 acccggcgac gaagtactcg ctcgtcaacc tgggcgaggc cctccggaac ccggagcaac
     3601 cgccggaggg aggactccgg gtctgcgaac ggtgcggcga gccgtgcggg gaggtctgcc
     3661 gggcctgcca ggtcctcgac gaggtgcgga ggggtgcctg aaggcccctc acagcatcgg
     3721 caggcaaccg ccggtcacgc cggcgaatat ctccatgaac tgttcggggt ggttgaggaa
     3781 ggcgaccgac cgggggcggc cgatcaggtc gtagagcccg
//
LOCUS       DAOSII010000172         3805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_21127_length_3805_cov_40.449067, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000172 DAOSII010000000
VERSION     DAOSII010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21127_length_3805_cov_40.449067"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PLV90_09600"
     CDS             <1..276
                     /locus_tag="PLV90_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase"
                     /protein_id="HOF97407.1"
                     /translation="EKIGRYPTFALWYTKDPLFDPRTRGAALLDEIVDAGRDLLSYER
                     VRVKVPQKWPWKAAVCTICGEMVPDNLLIDGACADCRSQSYYEKMPY"
     gene            342..1160
                     /gene="tsaA"
                     /locus_tag="PLV90_09605"
     CDS             342..1160
                     /gene="tsaA"
                     /locus_tag="PLV90_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOF97408.1"
                     /translation="MQRMEIFPIGIVHSSIRSRSDMPVQGVDAEIEVFPDYVAALDGI
                     KESSHLILICWLHEADRNVLKAVARKVSGDLPERGVFSLRSPVRPNPLSVSVVRLCGL
                     REGRILNLANVDLIDETPVIDIKPYQRGWDCIFSATGHDRTEKIRKMGPGEYRKSLIR
                     EAVNYHGELCPGVAVAVRIAEAAARILGCDLAVPEVAVAPGTDPCIADALIGITGARP
                     GNRRLRHPGGEDCVVAVPGVEVTFRLRDAPESVEAVLAAREASLFDCKIAFPGD"
     gene            1246..3072
                     /locus_tag="PLV90_09610"
     CDS             1246..3072
                     /locus_tag="PLV90_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HOF97409.1"
                     /translation="MSRRYLNLTPLPEALAAMRRAFPPSDHVETVPLCLAVGRVTAEP
                     LYAEYSVPEVEIAKFDGYAVKSRDTPGAQDQRPLPLREYARINTGEILPPSFDAVVMV
                     EDTWDDGGTPWIRKSAAPWQNVRHAGEDIRAGELALPKGHRIRPFDLGALATYGITRL
                     AVRSVRIGIIPTGNDLVPLGVVPAPGQTIETNTLMAEAYLTRMGATCRRYEIVPDDPD
                     LIRSALETALDANDLVILSAGSSAGSRDFSRDVVGALGEVIFHGIAVRPGKPVLLASV
                     GGKPVLGMPGYPVAAQTILREVAGSLLAWWGLPALPEGELDVRLSRRLASDLGFDEFV
                     PVSVGRVNGTCFATPHPRGGGIQMAVVRANGYIHIPAEREGFEAGEEVRVRLTVSPAE
                     IARTLICVGVRDPTITELGNCLADAGYLLHCCKASTMGAVLALRAKTCHAASISLPET
                     EAAWNDQVLRYLPGVDLLRVQVARTELGLASAGDLDAGNPASFRFINQPKGTAARVFL
                     DAWLAREGIDAGTVTGYGHEVRTPAAVAAAVGNGFADAGVCPAAMAREAGLRFTPLGY
                     ETRYLLVRKELAADEGVASLVRAARSPEFEEHLRSRERCRAG"
     gene            3304..3732
                     /locus_tag="PLV90_09615"
     CDS             3304..3732
                     /locus_tag="PLV90_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOF97410.1"
                     /translation="MPVSEQTQSQIMAVLRRMADAMGRKDIDGLVALVDPEFRAFGTG
                     PDEKAIGREAYRRHLERDFAQAEKVAIDLSDIRIGAEGTVAWAMADMAYHIVVGGIPQ
                     TLNGRMTAVLRGTGHAWIFVQMHYSLPAAGQEAGQSYPAV"
BASE COUNT          643 a         1213 c         1281 g          668 t
ORIGIN      
        1 gagaagatag ggcggtatcc gacatttgcc ctgtggtaca ccaaagaccc tctcttcgac
       61 ccgcggactc ggggcgctgc gcttcttgac gagattgttg atgccgggcg cgacctcctc
      121 tcgtacgaac gcgtgcgggt gaaggtaccg cagaagtggc cctggaaagc ggccgtctgc
      181 accatctgcg gtgagatggt ccctgacaac ctgcttatcg acggagcctg tgccgactgc
      241 cggtctcagt cttactacga gaagatgccc tactgacgga ggaaacccct ccctcttccc
      301 ccgtggaaag ggtctatgag ggtactggtt cattgtcaca gatgcagcgc atggagatct
      361 tccctatcgg gatcgtgcac tcgagcatcc gctcccggag cgatatgccg gtccagggcg
      421 tcgatgccga gatcgaggtc ttccccgact acgtagcagc gctggacggc atcaaagaga
      481 gctcgcacct gatcctgatc tgctggctcc acgaggcgga ccggaacgtc ctgaaggccg
      541 tggcccggaa ggtctcaggc gacctcccgg agaggggtgt cttctccctc cggtctccgg
      601 tgcggccgaa ccccctctcg gtgtcggtgg tgcggctatg cggtctccgt gaagggcgga
      661 tcctcaacct cgcgaacgtc gacctgatcg acgaaacacc ggtgatcgac atcaaaccct
      721 accagagggg atgggactgt atcttctccg cgaccggcca cgaccggact gagaagatac
      781 gtaagatggg gcccggagag taccgaaaaa gcctcattcg cgaggccgtg aactaccacg
      841 gggagctctg tccgggcgtc gcggtcgcgg tgaggatcgc ggaggcggcg gcgcgcattc
      901 tcgggtgcga cctcgctgtg ccagaggtcg cggtcgcgcc cggcaccgat ccctgcatcg
      961 ccgacgccct catcggaatc accggtgcgc gcccgggaaa ccgccggctg aggcacccgg
     1021 gtggagaaga ctgcgtcgtt gccgtgcccg gagtggaggt gaccttccgc ctccgggatg
     1081 ctccggagag cgtcgaggcc gtccttgcag cgcgcgaggc gtcgctcttc gactgtaaga
     1141 ttgcctttcc gggtgactga taggtaaacc aggagtaatg gagaatcgta tatcacgggc
     1201 gggggtaaaa accctccacc ggcacgctat aagggagtca tgagtatgtc acgccgatat
     1261 ctgaacctta ccccgcttcc cgaagcgctc gcggcgatgc ggcgggcgtt tcctccgtcg
     1321 gatcatgtcg agaccgtgcc gctctgcctt gccgtcggca gggtgacggc cgaaccgctg
     1381 tatgcagagt actctgttcc cgaggtcgag atcgcgaagt tcgacggcta tgcggtgaag
     1441 agcagggata ccccgggggc gcaggaccag cggccgctgc ctctccgcga gtatgcccgc
     1501 atcaacaccg gtgagatact ccccccctcg tttgacgccg tcgtcatggt cgaggatacc
     1561 tgggacgacg gcgggacacc ctggatcagg aagtctgccg cgccctggca gaacgtccgg
     1621 catgccgggg aggatatccg tgcaggcgaa cttgccctcc cgaaaggcca tcggatccgg
     1681 ccgttcgatc tcggcgcgct cgcaacctac gggataaccc ggctcgctgt ccggtcggtc
     1741 cggatcggca tcataccgac aggaaacgac ctcgtcccgc tcggcgtggt gcccgccccg
     1801 ggtcagacga tcgagacgaa caccctcatg gcggaggcgt acctcacccg gatgggggcg
     1861 acctgccgcc gttatgagat cgttcccgac gatccggatc tgattcggag cgcgcttgag
     1921 acggctctcg acgcaaacga tctcgtcatc ctctcggcag gctcgtcggc cggctcccgg
     1981 gacttctccc gcgacgtcgt cggcgcgctc ggggaggtga tcttccacgg catcgcagtc
     2041 cggccgggaa aaccggtgct gctcgcgagc gtcggcggca aaccggtcct cggtatgccg
     2101 ggctatccgg tcgccgcgca gaccatcctc cgggaggttg ccgggagtct gctcgcgtgg
     2161 tgggggctcc cggcgctccc ggagggggaa ctggatgtcc ggctgtcaag gaggctggca
     2221 tccgatctcg ggtttgacga gttcgtcccg gtctcggtcg ggcgggtgaa cggcacctgt
     2281 tttgcgaccc ctcatccccg ggggggcggc atccagatgg cggtggtccg ggcgaacggc
     2341 tacatccata tacccgccga gcgcgagggg ttcgaggccg gggaggaggt gcgggtccgg
     2401 ctcaccgtct ccccggctga aatcgcccgc acgctcatct gtgtcggtgt gcgcgacccc
     2461 accatcaccg agctcggcaa ctgccttgcg gatgccggtt acctgctcca ctgctgtaaa
     2521 gcctcgacga tgggggcggt gctcgctctg cgggcgaaga cctgccacgc cgcgtcgatc
     2581 tctcttccgg agacggaagc ggcctggaac gatcaagtgc tccggtacct gcccggggtc
     2641 gatctcctgc gggtgcaggt ggcccggacg gagctcggac tcgcctcggc cggcgacctg
     2701 gatgccggca accctgcgtc gttccggttc atcaaccagc cgaaaggcac ggcagcgcgg
     2761 gtcttcctcg atgcctggct ggcccgggaa ggcatcgatg cgggcaccgt caccgggtac
     2821 gggcacgagg tgaggacgcc tgcggccgtc gccgcagccg tcggcaatgg gtttgccgac
     2881 gcaggcgtct gtccggcagc catggcgcgg gaggcggggc tccggttcac cccgctcggc
     2941 tacgagacgc gttacctcct ggtccggaag gaacttgccg cggacgaggg tgttgcaagc
     3001 ctcgtccggg ccgcccggtc gccggagttc gaagagcacc tccggtcgcg ggagagatgc
     3061 cgggcagggt aaccggatac cctgcccgga gggacttaaa ggtgcgtggc tacaggaggt
     3121 tcatcggctg ccgcatccag agcgcaacgc gcctgaacag gccagaagct gcccggcaac
     3181 ccgccggcta tctcctgtgc ccgttgtgga taaccgtgat gcataagcct tccgttatcg
     3241 accgtccccg gggtggcgcg ccggtccgaa aagatcatct cctatcgggg gagagagttc
     3301 ttcatgccag tcagcgaaca gacacagagc cagatcatgg cagtgctccg gcggatggcg
     3361 gatgccatgg gcaggaagga catcgacggc ctggttgcgc tcgtcgatcc tgagttccgg
     3421 gcattcggga ccggtcccga cgagaaggcg atcgggaggg aggcctaccg ccggcacctt
     3481 gaacgcgact ttgcgcaggc ggaaaaggtc gcgatcgacc tctccgacat ccgcatcggt
     3541 gccgaaggaa cggtcgcctg ggccatggcc gacatggcat accatatcgt cgtcggcggc
     3601 atcccgcaaa ccctgaatgg gcggatgacg gcggtcctcc gggggacagg ccatgcgtgg
     3661 atctttgtcc agatgcacta ctctctcccg gcggcgggcc aggaggccgg acagtcgtat
     3721 ccggcggtat aattttttgc ccgccgaggg ccctgtgccg gtttcggcgc tcgttatagg
     3781 gaataccgat acgttctcct ccggg
//
LOCUS       DAOSII010000173         3792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_21201_length_3792_cov_48.568103, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000173 DAOSII010000000
VERSION     DAOSII010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21201_length_3792_cov_48.568103"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="PLV90_09620"
     CDS             complement(<1..1157)
                     /locus_tag="PLV90_09620"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOF97411.1"
                     /translation="MNPSDLVCFLKKDPAEFTREDIIRFCEANRIEMVNFRYAAEDGK
                     LKTLNFVISSKEHLDTILSDGERVDGSNLFSFIEAGSSDLYVIPRYRTAFVNPFAEVP
                     TLELLCSFYDSDGKPLESSPEYVLRKADEEFTRRTGCTFKTLGELEYYVVSPRGDLYP
                     GRDQKGYHSADPFVKFADLRDEAMRLIARAGGMIKYGHSEVGCFFAGDTYYEQHEIEF
                     LPMPVEQAVEQVIIAKWILRMLGNRYGVEISFAPKITVGKAGSGMHFHMLAEKDGQNL
                     MVEGGRLSPLARKMIAGILDVSDALTAFGNTIPTSYLRLVPHQEAPTRICWGDRNRSV
                     VVRVPLGWIGAEGMTHDANPCENRHRDERSSKQTFEYRVADGSADPYLTVAG"
     assembly_gap    1427..1526
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1527..2467)
                     /locus_tag="PLV90_09625"
     CDS             complement(<1527..2467)
                     /locus_tag="PLV90_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HOF97412.1"
                     /translation="MCDKDPDRHARPRMVLPCALLLTCLALFFTIVPVGLTGAPPARE
                     QPDPAIVEIIGEVNESELWNTTYDLQAIPTRVFGSEGNREAGEYLYARLDGIPGLDVE
                     FQGGELNNVVATLPGSGNASSELVIVGAHYDSTSSDPARAPGATDNACGVAIVMELAR
                     VMSGHSFDRTVQFAFWNAEEVGSYGSIDYATHAMDTGVPVVLYLNYDSACYDPENRFI
                     LDIMYDSGSADIAALMADHNTLYGTNFTLTRNRHACESDHTSFQALGFPALMTHSEEH
                     GPAHTPEDTIDHVSSGYMAENARLGLSVLAKVAGPRDA"
     gene            2723..3154
                     /locus_tag="PLV90_09630"
     CDS             2723..3154
                     /locus_tag="PLV90_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF61 family protein"
                     /protein_id="HOF97413.1"
                     /translation="MPDRPSAGDESTLLRWMRLEIGRINDGIVSERKRLSGLLSEERP
                     SSVTKGGSEYGFDRDILHRLEQALPGELQRRLRLPILFYFDSTVPDSLFLADEAAMQA
                     LQSLGEISPLRRMQGGRLWIAKPLVYAIMNRYPTVMQVVMG"
     gene            complement(3238..>3792)
                     /locus_tag="PLV90_09635"
     CDS             complement(3238..>3792)
                     /locus_tag="PLV90_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97414.1"
                     /translation="TMSEKELKIGDIAHLTGISEQDVRTLVRTYESLFSYRTIGPVRL
                     YPEKAVAIIREILELSGKGLTPGEIAEEVGTGRRSGAQEEPAEEVGRTGAALPPGLVF
                     DLPVIQDTLARQERWIARLTDELEREREEVARLQDAVDHLQADLDRQQGQLAVVAEWV
                     DYFDRRMDEVARPVLERFRQTFGR"
BASE COUNT          656 a         1152 c         1176 g          708 t
ORIGIN      
        1 ccggcgacgg tcaggtaggg gtcggccgaa ccgtcggcga cccggtactc gaacgtctgc
       61 ttcgacgacc gttcatcgcg gtggcggttc tcacagggat tggcatcgtg tgtcatacct
      121 tccgcgccga tccagccgag aggcacgcgg accacgaccg agcgattgcg gtcgccccag
      181 cagatccgtg tcggcgcctc ctggtggggg acaaggcgga ggtaggaggt cgggatggtg
      241 ttgccgaagg ccgtcagggc atcggagacg tcgagtatgc cggcgatcat cttccgggca
      301 agcgggctta acctgccgcc ctcgaccatc aggttctggc cgtccttctc ggcgagcata
      361 tggaagtgca tgccgctccc ggccttgccg acggtgatct tcggggcgaa gctgatctcg
      421 acgccgtacc ggttgccgag catccggagg atccacttgg cgatgatcac ctgttcgacc
      481 gcctgctcca ccggcatcgg caggaactcg atctcgtgct gttcgtagta ggtgtcgccg
      541 gcgaagaaac acccgacctc cgagtggccg tacttgatca tgccgccggc ccgggcgatg
      601 aggcgcatcg cctcatcgcg caggtccgca aacttgacga aggggtcggc cgagtggtag
      661 cccttctggt cgcggcccgg gtagagatcc ccccgcggac tgacgacgta atactcgagt
      721 tcgccaaggg tcttgaaggt gcacccggtt cggcgggtga actcttcgtc tgccttgcgg
      781 agcacgtact cgggcgaact ctcgagcggt ttgccgtcgc tgtcgtagaa ggagcagagc
      841 agttcaagtg tcggcacctc ggcgaaggga ttgacaaatg ccgtgcggta gcgcgggatc
      901 acgtagaggt cgctgctccc cgcctcgatg aacgagaaga gattgctgcc gtcgacgcgt
      961 tcgccgtctg agagtatggt gtcgaggtgt tcctttgagg agatgacgaa gttgagggtt
     1021 ttaagcttgc cgtcttcggc ggcgtagcgg aagttgacca tctcgatccg gttcgcctca
     1081 cagaaccgga tgatatcctc cctggtaaac tccgccggat ccttcttgag aaaacagacc
     1141 agatcgcttg ggttcatcag gatcagagaa tctctcatat ttttttaacg ttgatctcag
     1201 gtatataaaa agcgtactct ccacctcata ccacctcgat cgggggccgg aggtttccgg
     1261 cgttcgggaa gatttccggg cctcacccgg aactgcggca ggtgcgtatc ccgggtgcgg
     1321 gggtacggag agccccggga acgcacccgc tctgtgccgc gcgaggagcg gcaccgatcc
     1381 gcccgcggga ttcacggagg cccggcaccc ggtgcggatg aatcccnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnngcgt cccggggacc ggccaccttc gcgagcaccg
     1561 agaggccgag ccgggcgttc tccgccatat acccggacga gacatggtcg atggtatcct
     1621 ccggggtatg ggccgggccg tgctcctcag aatgcgtcat cagggccggg aaccccaggg
     1681 cctggaaaga tgtatggtct gattcgcagg cgtgcctgtt ccgggtcagg gtgaagtttg
     1741 tgccgtagag ggtgttgtga tccgccatga gcgccgcgat atccgcggat ccgctgtcgt
     1801 acatgatatc gaggatgaag cggttctccg ggtcgtagca ggccgagtcg tagttgaggt
     1861 agagcaccac cgggactccc gtatccatgg catgcgttgc atagtcgatg ctgccgtatg
     1921 agccgacctc ttcggcgttc cagaacgcga actgcaccgt ccggtcaaac gaatgcccgc
     1981 tcatcacccg ggcaagttcc atgacgatcg cgactccgca tgcgttgtcg gtcgccccgg
     2041 gcgcccgggc cgggtccgat gacgtgctgt cgtagtgcgc tcccacgatg acgagctcgc
     2101 ttgaagcgtt gccgcttccc gggagcgtgg caacgacgtt gttgagttcg cccccctgaa
     2161 actccacatc gagcccgggg atgccgtcaa gccgtgcgta aaggtactcg cccgcttccc
     2221 ggttcccctc cgacccgaac acccgggtcg ggatcgcctg caggtcgtat gtcgtgttcc
     2281 agagttcgga ttcgttgacc tccccgatga tctccacgat ggcaggatcg ggttgctcgc
     2341 gcgccggggg tgcgccggtg aggcccaccg gaacgatcgt gaagaagaga gccaggcagg
     2401 tgagcagcag ggcacagggg agcaccatcc gggggcgggc atgccggtca gggtctttgt
     2461 cgcacatacg atcacatccc atgagagata cgcagaaggc gttgcatggt gagcgatgac
     2521 acatatacct tcgccagggt ggcgggaggg ttatggtacg aggaggggcc ggattatccc
     2581 ggacaacgag aggtatatca ccgggcagtt tttgaggatc ccccgagatt cctcccgaat
     2641 cccggaacca gaggtatata ccgtatgacg ttgactgatg cgtatactcg ccggaaccag
     2701 gccatcaccg gagcgcacaa gtatgcccga ccgaccatca gcaggcgacg agtcgaccct
     2761 cttacgatgg atgcggcttg agatcggcag gatcaatgac ggtatcgtct cagagcgcaa
     2821 acgcctctcc ggacttcttt cagaagagcg tccttcgtcc gtcacgaagg gcggcagcga
     2881 gtacgggttt gaccgggata tcctccaccg tctggagcag gccctccccg gagaactgca
     2941 gagacgactc cggctcccta tcctcttcta cttcgattcc acggtcccgg atagcctctt
     3001 ccttgcggat gaggccgcga tgcaggccct ccagagcctc ggagagatca gccccctccg
     3061 gaggatgcag ggcgggaggc tctggatagc aaaacctctc gtctacgcca tcatgaacag
     3121 gtacccgacg gtgatgcagg tcgtcatggg gtaaatagcc tctctccggc agccgccacc
     3181 acggcggccc gccctcagtg gcgcggccga cggtacggta tgcggccgcc gaccgcctca
     3241 tctcccgaac gtctgccgga atcgctcgag caccggacgg gcgacctcat ccatccgccg
     3301 gtcaaagtag tccacccact ccgcgacgac cgcaagctgc ccctgctgcc tgtcaaggtc
     3361 cgcctgcagg tggtcgactg catcctggag ccgggcgacc tcctccctct cccgctcaag
     3421 ttcatccgtc aggcgtgcta tccagcgttc ctgccgggcc agcgtgtcct gtatcaccgg
     3481 gaggtcgaac accagccccg gcgggagcgc cgcgccggtt ctgccgacct cttccgccgg
     3541 ctcctcctgc gcgcccgatc tcctgccggt gccgacctct tcagcgatct cccccggcgt
     3601 gaggcctttc ccggagagtt ccaggatctc acggatgatt gcgaccgcct tctcggggta
     3661 gagcctgacc gggccgatgg tccggtagga gaagaggctt tcgtaggtcc gaacgagcgt
     3721 ccggacatcc tgctcggaga tcccggtgag gtgcgcgata tcgcctatct tgagttcttt
     3781 ctcgctcatg gt
//
LOCUS       DAOSII010000174         3732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_21670_length_3732_cov_47.686429, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000174 DAOSII010000000
VERSION     DAOSII010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21670_length_3732_cov_47.686429"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PLV90_09640"
     CDS             <1..384
                     /locus_tag="PLV90_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HOF97415.1"
                     /translation="CIDREVFSRLDALEVICGGMARSHNLKAAALATAHGLGRTGGTD
                     GHLLHELGGVVTCAEAETVEELLDAIRARATVIIGRERPLVEKAVMGTAVLPHHLPHT
                     VPILQARWEQNLPRIKRFIKAHLDG"
     gene            complement(391..1824)
                     /gene="cls"
                     /locus_tag="PLV90_09645"
     CDS             complement(391..1824)
                     /gene="cls"
                     /locus_tag="PLV90_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOF97416.1"
                     /translation="MTFDILFVIIIVLNILFAVTIVFFERKNPTAAMAWLMVLFFLPP
                     IGFFLYLFFGQNYTRQRMFVVKREEDRRFLQEIFLAQHREFADTRYRFAIPEVDEFRE
                     AILLLLQNNRAFLTGKNRVDLYTRGEDKFDALFAAIREARHHIHLEYFILNNDELGRA
                     VVHALAEKALEGVEVRLLFDAMGTRAGGGTQKAFSELTDAGGKIGVFFPSVYRVNYRN
                     HRKIAVIDGTVGFIGGFNIGDDYLGKGPLGPWRDTAFRIAGQAVQMLQLRFFLDWHYV
                     TGGYPGPRPRYFPAPGDHGTTPIQIVSSGPDTRWSPIKEGYIKLINSARESVYIQTPY
                     FVPDESVTDALRIAALSGVDVRVMIPCKPDHPFVYWASLSFIGDLLDAGVRAYTYDNG
                     FLHAKTIVVDGKAGSVGSANWDVRSFRLNFEANAFFYDAAVGAELARAFKEDLALSTE
                     ITLESYRARSRRVRMKESVSRLFSPLG"
     gene            complement(1912..2451)
                     /locus_tag="PLV90_09650"
     CDS             complement(1912..2451)
                     /locus_tag="PLV90_09650"
                     /inference="COORDINATES: protein motif:HMM:NF018410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HOF97417.1"
                     /translation="MFLTGVLIWLHQAVKALAALLILVTLLPLVLGLVLGIPAARVLG
                     LISSTIVLQANAVFVGVGMGIHPAVVLLIMTLVEVGVVLAIYSICDAFALQSARVRGM
                     LERTEEKIQKVPLLARYGAVTLVVLPAMPIIGLYSSTVISWILGWNRMLSLIFITIGW
                     VAVTAFLLLVALGFVQVLF"
     gene            2653..3150
                     /locus_tag="PLV90_09655"
     CDS             2653..3150
                     /locus_tag="PLV90_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HOF97418.1"
                     /translation="MANTTMLIQGIVALALGVLLLTYPVGVLMILTIILGIYWLIDGI
                     VVLTNLYSDRTDKGWKVLVGVLGIIAGILVLAYPLYSAILLPTFLAIIIGVEGLIIGV
                     VHLVRGVTGAGFGSGVLGIVSIIFGLILIAHPFIAALALVWVLAIIAIAGGILAIAFA
                     LRKSA"
     gene            3368..>3732
                     /locus_tag="PLV90_09660"
     CDS             3368..>3732
                     /locus_tag="PLV90_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HOF97419.1"
                     /translation="MLDRMKESSGPVIGFRFDGKLSESDYTTLLIPELERAMKDHRNV
                     RVLLKIEGFRSWRPGEPWEAFRQWSGVESVDRVAVVGGERWREWMNRLPGLFVGFSGI
                     DVRYFPENRLRDAWGWLREG"
BASE COUNT          749 a         1156 c         1158 g          669 t
ORIGIN      
        1 tgcatcgaca gggaggtctt ctcccgcctc gatgccctcg aggtgatctg cggtggtatg
       61 gcccggtccc ataacctgaa ggcagccgct cttgccaccg cccacggcct cggccggacc
      121 ggcgggaccg acggccacct cctccacgag ctcggcgggg tcgtcacctg cgcggaggcc
      181 gaaaccgttg aagaactcct cgacgccatc cgggcgagag cgaccgtcat catcgggcgc
      241 gagcggccgc tcgtcgaaaa ggccgtgatg gggaccgcag tcctcccgca ccacctcccc
      301 cacaccgtcc ccatcctcca ggcacgctgg gagcagaacc tcccccggat caagaggttc
      361 atcaaggccc acctcgacgg gtgaggtggc tcacccgagg ggcgagaaga ggcgggagac
      421 cgactccttc atcctgaccc ggcgcgaccg cgcccggtaa ctctccagcg tgatctcggt
      481 ggagagagcg agatcttcct tgaatgcccg tgcaagttcg gcgccgaccg ccgcatcgta
      541 gaagaaggcg ttcgcctcga agttcagccg gaaactccgc acatcccagt tcgcgctccc
      601 gaccgatccg gccttcccgt cgacgacgat cgtctttgcg tggagaaacc cgttgtcgta
      661 tgtgtaggcc cgcaccccgg cgtcgagaag gtcgccgatg aacgagaggc ttgcccagta
      721 gacgaaaggg tggtcgggtt tgcaggggat catgacgcgg acatcgaccc cggagagcgc
      781 tgcaatccgt agagcatcgg tgacactctc atcgggcacg aagtagggcg tctggatgta
      841 gaccgactcc ctggccgagt tgatcagctt gatgtaaccc tccttgatcg ggctccaccg
      901 ggtatcgggg ccgctcgaga cgatctggat cggcgtcgtc ccgtggtcgc ccggggccgg
      961 gaagtagcgt gggcggggcc cgggataccc gccggtcacg tagtgccagt caaggaagaa
     1021 ccggagctgg agcatctgga cggcctgccc ggcgatccgg aaagccgtgt cgcgccaggg
     1081 cccgagcgga cccttcccca ggtaatcgtc cccgatgttg aacccgccga tgaaccccac
     1141 cgtcccatcg atgaccgcga tctttcgatg gttgcggtag ttgacccggt agaccgaggg
     1201 gaagaagacc cctatcttac cgccggcatc ggtcagttct gagaacgcct tctgcgttcc
     1261 gccgccggcg cgggttccca tcgcgtcgaa gaggaggcgg acctcgacac cctccagcgc
     1321 cttctctgca agggcgtgga cgaccgcccg ccccagttcg tcgttgttga ggatgaagta
     1381 ttccaggtgg atatggtggc gcgcctcccg tatcgcggca aagagcgcgt cgaacttatc
     1441 ttcaccccgg gtgtagagat caacccggtt cttcccggtc aggaacgccc ggttgttctg
     1501 gagaaggaga aggatcgcct cgcgaaactc atccacctca gggatggcga accggtagcg
     1561 agtatcagca aactcacgat gctgcgcgag aaatatctcc tgcaggaacc ggcggtcttc
     1621 ctccctcttt acgacgaaca ttctctgccg ggtataattc tgcccgaaga agagatagag
     1681 gaagaaccct atcggcggca ggaagaagag gaccatcagc caggccatcg ccgccgtcgg
     1741 gttcttccgc tcaaaaaaga cgatggtgac ggcaaatagg atgttaagaa ctattataat
     1801 gacaaatagt atatcaaagg tcatcctgtt gttctcccct tgccagagtc gagcatgatc
     1861 actctatctc tccagggata tccatctacc gaacggtata tcgccggccc gtcagaagag
     1921 cacctgcaca aacccgagcg cgacgagcag gaggaaggcc gtgacggcga cccacccgat
     1981 ggtgatgaat atgagggaca gcatcctgtt ccacccgagg atccagctga tcaccgtgct
     2041 cgagtagagg ccgatgatcg gcattgccgg gagaacgacg agggtcaccg caccgtacct
     2101 cgcaagaagc ggcaccttct gtatcttttc ctccgtgcgc tccagcatac ccctgacccg
     2161 tgccgactgg agggcaaacg catcacagat cgagtaaatg gcaagcacga cgccgacctc
     2221 gacgagcgtc atgatcagaa gaaccacagc agggtgtatc cccataccca cgccgacaaa
     2281 aacagcgttt gcctggagaa cgatggtgga tgagatcaga ccaagtaccc gggccgccgg
     2341 tatgccgaga accaggccga gaacgagggg gaggagcgta acgaggatga gcaatgccgc
     2401 cagcgccttc acagcctgat gaagccagat cagcacaccg gtgaggaaca tgcacgacga
     2461 ccctgccggg caggggaaag gcaggtgtgc acatataact tcctgtatca cgactgcccg
     2521 gcagcatgcg ccggagcacc cgggcccggg cgttccgggg aacttccgag tcagtctctg
     2581 gccgatctca tgggagatct gttgccgaag aggtgcatat ttataccagg attagaccac
     2641 ctgaggtatt atatggcaaa cacaaccatg ctgatccagg gcatcgtcgc ccttgccctc
     2701 ggggtcctgc tcctgacgta ccccgtaggg gttctcatga ttcttaccat aatcctcggc
     2761 atatactggt tgatcgatgg aatagtagtc ctcacgaacc tttactcgga cagaacggac
     2821 aaggggtgga aggtgctcgt cggcgtcctc ggcatcatcg ccggcatcct ggtcctggcc
     2881 tatccgctct acagcgccat cctccttcca acgttccttg ccatcatcat cggtgtcgaa
     2941 ggcctgatca tcggggtggt ccatctcgtg cggggagtca ccggcgccgg tttcggatcc
     3001 ggggttctcg gtatcgtgag catcatcttc gggctcatcc tgatcgcgca tccgttcatc
     3061 gccgctctcg ccctggtatg ggttcttgcg atcatcgcca tcgccggagg gatactggca
     3121 atcgcctttg cactccggaa gtccgcgtga ccggaccgcc cgccgtgcgg agcatgcccg
     3181 accgggagcc actccgggca cccggacgat gggccggtac ggccctccgc ggccggggcc
     3241 agaccggggc gaacgggctc cgggttcctc cggggagaga ggtggaaccg gtacgccggt
     3301 gacacagcta ctcgctcccg ggaggagtgc cgcagccttt ttctcttccg ggtgcaaaag
     3361 ggcacccatg cttgaccgga tgaaggagag ttcgggaccc gtgatcgggt ttcggttcga
     3421 cggaaaactg agcgagagtg actataccac cctcctgatc ccggagctcg aacgagcgat
     3481 gaaagaccac cggaacgtcc gggtcctctt gaagatcgaa gggttccgga gctggaggcc
     3541 gggagagccc tgggaggcgt tcaggcagtg gtccggggtg gagagtgtcg atcgggtcgc
     3601 cgtcgtcggc ggcgaacggt ggcgggagtg gatgaatcgc ctgccggggc tctttgtcgg
     3661 gttctccggc atcgatgtcc gctacttccc ggagaaccgc ctccgtgacg cctggggatg
     3721 gctccgggaa gg
//
LOCUS       DAOSII010000175         3708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_21848_length_3708_cov_41.344922, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000175 DAOSII010000000
VERSION     DAOSII010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21848_length_3708_cov_41.344922"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            447..1604
                     /locus_tag="PLV90_09665"
     CDS             447..1604
                     /locus_tag="PLV90_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97420.1"
                     /translation="MSTGALDWLEEHFPRDFFDETVAQRALRHLGFNEKRALFSDDYV
                     LQRTSEGFWYEFIVYEIMLELSLETDRIQSIVGKGADASAYSVGMRPGQNGFYYSERG
                     SLTVRGNGQNIAEMDLLFVDGRGNMGFVEVITSAMELEKFYEEIHYKKTLLASLFGQE
                     TVPFVLVSSVDVSRYDSILRIAEMPDSLILITNPMEEIRTLIYEGSLRRRPEKPGHHP
                     KRIDLTSIRPRRPFDYKEIHDRNRDVVIRTLLSCRDGGIAVISSAINSLSKKIMLGTL
                     GEAGVKAVLRDRKVRIKDTTLYADDIKQRFSRVVIAFDIPAYTPVIYFKLKEKEVYLK
                     LIQNASGDLVFQGKRTPAPYMSGFYEWLDADKPTVDSRVAERYASLFLGTS"
     gene            1895..2590
                     /locus_tag="PLV90_09670"
     CDS             1895..2590
                     /locus_tag="PLV90_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOF97421.1"
                     /translation="MEEGPRGAITQRDRTSYAIVPRTPAGIISPEHLESIIKVVRRYN
                     IPVIKMTSGQRMALVGIKKEDVEKIWNELGMTVGQATAPCVHYVQACPGTVTCKYGKQ
                     DALGLGLEVEELYQDMNLPAKLKMGVSGCPRCCGESYLRDIGLIGDAKGWNVIFGGNS
                     GGRPRIGDVAARGLSKDEALDLVRRLLDYYRKNARPGERTARFVERVGFDTIKSDVLT
                     LAPYIPLDSIRSR"
     gene            3041..>3708
                     /locus_tag="PLV90_09675"
     CDS             3041..>3708
                     /locus_tag="PLV90_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2115 domain-containing protein"
                     /protein_id="HOF97422.1"
                     /translation="MQFRVFLNRLLRLIGRGGRSEPGADPDDACALIIDPFAGDPGEE
                     VGEGSIPVDAIRAACRRMHGAATKGELLRIVAGEVAAFDLRDLEAMNAGFERKVANLP
                     PDYRDRLLASVREEIFSAHHRLVLLSRNGSNPGMDEPPGPGHQTYWAMVAEACTAKAR
                     EKDPKYLYLKYLLSGFTMFVLKEPAHPVGTPFPGGQIVDEWEGTYLCPVRDKADDVAF
                     ALCP"
BASE COUNT          779 a         1183 c         1086 g          660 t
ORIGIN      
        1 gcaatgcctt tgctgcccca tatcgcggca tactccgggt cgatctccag cgcgtggtca
       61 taacaccgga tcgccccgtc acgatcccct gagatgtgaa gagcaatgcc ctcgttatac
      121 cagtctatcg catccctttg ctgcatgagt tctcttcctc ctttgctgct gtcgcaacgt
      181 atgccggata gaccacccgg aacaccgcca gggtgccagt cgagcagttg cctgacgttc
      241 attcgttgtt acccggcgag cataacccta tccattacgc cgccggcgca tcggggcatc
      301 ctcgctgagc aacaaccatg gcaacaaatc gcatgatgaa tctgtctccc tcatcggtag
      361 ttccccggag cactacccac cctgtacccc cctgcccggg ccggccggac gaaaatagta
      421 atatacctcc agtggtatcc tatcatatga gcacgggcgc cctggactgg cttgaagagc
      481 actttccccg ggattttttt gacgagacgg ttgcacaaag agctcttcgc catctcggat
      541 ttaatgagaa gcgtgccctc ttctccgacg actacgtgct ccagcgcacg tccgaggggt
      601 tctggtatga gttcatcgtg tacgagatca tgctggagct ctccctcgag accgaccgga
      661 tacagtccat cgtgggaaag ggagccgatg cgtcggcata ctctgtcggg atgcgccccg
      721 ggcagaacgg gttttattac tcggagcgag ggagcctgac cgtcagagga aacgggcaga
      781 acatcgccga gatggacctc ctgttcgtgg acggccgtgg gaacatgggc tttgtcgagg
      841 tgatcacctc cgcgatggaa ctggagaagt tttatgaaga gatccactac aagaaaacgc
      901 tgctcgcctc tctgttcggg caggagaccg tacccttcgt cctggtctcg tcggtggatg
      961 tctccaggta cgacagcatc ctcaggattg cagagatgcc cgacagtctc atcctgatca
     1021 ccaaccccat ggaagagata cgaaccctca tctacgaggg gtcgctgcgg aggcgcccgg
     1081 agaaacccgg ccatcaccca aaacggatcg atctcaccag tattcgcccg agaagaccct
     1141 tcgactacaa agagatccac gaccgcaaca gggatgtggt catcagaacc ctgctctcct
     1201 gcagggatgg cgggattgct gtgatatcat ctgccatcaa ctcgctctca aagaagatca
     1261 tgctgggcac gctgggggag gccggcgtca aagcggtgct gcgcgacaga aaggtcagga
     1321 taaaagatac caccctgtat gccgacgaca taaaacaacg gttcagcagg gtggtgatag
     1381 cgtttgacat tcccgcgtat acgcccgtca tttacttcaa actgaaagaa aaggaggtgt
     1441 acctgaaact catccagaac gcctccggag acctggtgtt ccagggtaag cggacgcctg
     1501 caccctacat gtcggggttc tacgagtggc tggatgccga taagcccacg gtcgactccc
     1561 gggttgcaga gcggtatgcc tcgctctttc tgggcacaag ctgaacgaaa attcccgggg
     1621 aacgcatctt tgaaaaacca cacgggatcc ctctcatata cgccggccgg cctcctcgcc
     1681 cgcccgaaaa tcccagaaaa cctcttcgcc gcgaaggtcg tgcccctgac ggcaaccgca
     1741 tacctcccgc acccccgctc acggaacaga ggaccatacg gggatccctg aggggacggg
     1801 ccttagagag cagcggcact cttttccctc aggagaacga aggttggatc atatccggcg
     1861 cacctgactt tgccggaaaa acgagaggca tactatggaa gaaggaccaa ggggagcaat
     1921 cacccagaga gacaggacat cctacgcgat cgtgccgaga acccctgcgg gaatcatcag
     1981 ccctgagcac ctggagagca tcatcaaggt cgtcaggcgc tacaacatcc ccgtcatcaa
     2041 gatgacgtcg gggcagcgga tggcgctcgt gggcatcaag aaagaggatg tcgagaagat
     2101 ctggaacgaa ctcgggatga ccgtcggcca ggcgactgcg ccctgcgttc attacgtcca
     2161 ggcatgcccc gggacggtga cctgcaaata cggcaagcag gacgccctgg gcctcgggct
     2221 tgaggtcgag gaactctacc aggacatgaa cctgccggca aaactcaaga tgggagtctc
     2281 cgggtgcccc cggtgctgcg gcgagagtta cctccgcgat atcgggctca taggggacgc
     2341 aaaaggctgg aacgtcatat tcgggggcaa ctccggcggc cggccgcgta tcggcgacgt
     2401 cgctgccagg ggcctctcga aagatgaagc tctcgacctc gttcgccggc tgctcgatta
     2461 ctaccggaag aacgccagac cgggagagcg gaccgcacgg ttcgtcgagc gggtcgggtt
     2521 tgacaccatc aaaagcgatg tcctgaccct cgctccctac ataccgctgg actccatccg
     2581 gtccagataa ggggttgtgc gggagcgggg actcccatct cccccgggcc gccgacttgc
     2641 ccggcgttcc ggaccccgcc cggccgggag acgatgcgtt gagcgatctg agaatggagc
     2701 gcgaacaccc ctccctcatc atcccgggcg ccgggcgatc cccgcaaaga ccttcttgaa
     2761 cggccgggac agcagcccca tagtcgcgag gcccgccggc ggtcgagagg ccccgccatg
     2821 ggtattcatg gagcgggccc ggatgtaaca tcggccagac cctctgtcac tcccggcagg
     2881 cgaccatgca ggcccgcggg cgggcacccg cctcgccgat cccggcaaag gccgcatggc
     2941 ctcgtacccg cgagccgggt tatccacact cttgcccggg gattacgcca ggattatacc
     3001 tcttcacgcc ccatcttttc cagagaactc gggtgacatc atgcagttcc gcgtgttcct
     3061 gaaccgtctc ctgcggctca tcggccgggg aggccggagt gagcccggcg ccgacccgga
     3121 cgatgcctgc gccctcatca tcgacccctt cgccggcgac cccggtgagg aggtcggcga
     3181 gggatcgata ccggtagatg ccatccgggc ggcctgccgg cggatgcacg gggcggcgac
     3241 gaagggagaa ctcctccgca tcgtcgccgg cgaggtagcg gcattcgacc tgcgcgatct
     3301 cgaggcgatg aacgccgggt ttgagcgcaa agtcgcaaac ctccccccgg actaccgtga
     3361 ccgcctcctc gcaagtgtca gggaggagat cttctcggca caccaccgcc tggtcctcct
     3421 ctcccggaac ggcagcaatc ccggcatgga tgagcccccg ggcccggggc atcagaccta
     3481 ctgggccatg gtggccgagg cctgcaccgc aaaggcgcgg gagaaagacc cgaagtacct
     3541 ctacctgaag tatctcctct ccgggttcac catgttcgtc ctcaaagagc ccgcccaccc
     3601 ggtcggcact ccgttccccg gcgggcagat cgtcgacgag tgggagggaa cttacctctg
     3661 cccggtcagg gataaggccg acgacgtggc tttcgccctc tgccccta
//
LOCUS       DAOSII010000176         3704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_21885_length_3704_cov_51.570019, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000176 DAOSII010000000
VERSION     DAOSII010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21885_length_3704_cov_51.570019"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..1715
                     /locus_tag="PLV90_09680"
     CDS             66..1715
                     /locus_tag="PLV90_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOF97423.1"
                     /translation="MPSGEPHWRKSLSADIVAGTTTALVGIPQTMGFALVAGINPIYG
                     LYTAAFSTAFGALLTGSSFLKVMLSNVIAVSLFSILAPVPESDVPATLFVLTLLVGLF
                     QLGFGLARAGSLTRFISNAVLTGFIIGAALLIVLGQLGNLTGYELPGDAIQILAVLDL
                     ILHAGDIQVQPLAVGLLTIALALTFRRAPRLSSAALLLPIVITTLLVRAADLEVALVG
                     DISAIPAGLPFPVLPELALAPGLLVPALALAIIGLVMAIGITEKTPEPDGTIADVNRD
                     FAGQGVANILASFFQCAPSSGSLSATTLNVSAGAETRAANLISGILVGAVILVAGPLA
                     ELIPLSALAGILILIGAELIYRPHEIVCICRYSRPGRWGMIATFISTQVLPLQYSIYV
                     GVFLSLAIYLITSTREASVVRLVPVEGGMFREESPPGWLPDGRLTILSIAGSVYFATL
                     RAIERSLPSPEGTDGAVVILALRGRVEAGTGFFRMLERYARRLLAHNGRLILAEVDPR
                     LLAGLAETGVTETVGPANIFVATPTIWESLMEAIQTAEAGT"
     gene            1770..2792
                     /locus_tag="PLV90_09685"
     CDS             1770..2792
                     /locus_tag="PLV90_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOF97424.1"
                     /translation="MNPESLSPPARIAIVGAAVVIVLAGIQAATPILGPFLIAVFFAM
                     ITAPIMAWLTRRGVPRALAAGMVVTGLAVVLVGTIAFLGMAFTEFLLSLPRYEKALEA
                     QATIIASYGIDTTGIQVWDYIDRGFVIQQAAEIARQVGNIAFDAIIVAIAIGFLLIEA
                     PRFAAALARRLGPESPPYRHLTQSGRFLIEYVVIRTEVNLVTGVGTGLFLALLGVEFA
                     ALWGFIAFILSYVPYIGLVVAAIPPTLLALVGFGPSGALAVIVAITLIDAAAENLVFP
                     QITRKGLNLSPFVVIFSVVFWGLVLGAVGVFLAIPLTIAVKLFLESWDETRWLGEMMN
                     AGERQK"
     gene            2924..3325
                     /locus_tag="PLV90_09690"
     CDS             2924..3325
                     /locus_tag="PLV90_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HOF97425.1"
                     /translation="MLERMETGAGEIAGFRFDGDMTTKDYNGVLIPALEEAGGTHPVF
                     RILFSIVNFHGWKPRGNWEALKDWPGMEKVDRIAIVGGEKWEEWMNYLPGLFVGFVGI
                     DVRYFSSEHLNDALDWLREPIAAEEAALKPG"
     gene            3415..>3704
                     /locus_tag="PLV90_09695"
     CDS             3415..>3704
                     /locus_tag="PLV90_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOF97426.1"
                     /translation="MRHYKDIIFEKPLPEAIRRLGLLPADMHFHTRHSDSPTRVRDAL
                     KLAARRGIGLAITDHNQVGGVVEAGRQNIRVPLVPGVEVSANDGPHILLYFYS"
BASE COUNT          590 a         1335 c         1087 g          692 t
ORIGIN      
        1 ctccaccctt gccgggcaag cccgaagagt aactatatac ccggcggccc gcactctccc
       61 ggcgcatgcc ctcgggtgaa ccgcactgga ggaagagtct ctccgccgac atcgtcgccg
      121 ggacgaccac tgccctggtc ggcatcccgc agacgatggg gtttgccctc gtcgccggta
      181 tcaacccgat ttacggcctc tacaccgccg cgttctcgac ggcgttcggg gcgctcctca
      241 ccggctcctc gttcctcaag gtgatgctct caaacgtcat cgcggtctcg ctcttctcca
      301 tcctcgctcc ggtcccggag agcgatgtcc cggccaccct ttttgtcctg acgctcctcg
      361 tcggactctt ccagctcggg ttcggtctcg ccagggccgg cagcctgacc cggttcatat
      421 cgaacgccgt cctcaccggg ttcatcatcg gagcggcact cctgatcgtc ctcgggcagc
      481 tcgggaacct caccggctac gaactcccag gggacgcgat ccagatcctt gccgtcctcg
      541 acctcatact gcatgcgggc gatatccagg tgcaaccgct tgccgtcggc ctcctcacca
      601 tcgccctcgc cctcaccttt cggcgggcgc cgcgcctcag ttccgccgcc ctcctcctcc
      661 cgatcgtcat aacgaccctc cttgtacggg cagcggatct cgaggtcgcg ctcgtcggcg
      721 atatcagcgc catccccgcc ggcctcccgt tccccgtcct ccccgagctc gccctcgctc
      781 ccggtctcct cgtcccggcg cttgccctcg ccatcatcgg tctcgtgatg gccatcggga
      841 tcaccgagaa gactcccgag cccgacggga ccatcgccga cgtcaaccgt gactttgccg
      901 ggcagggggt ggcgaacata ctcgcaagct tcttccagtg tgcaccctca agcggctcgc
      961 tctcggcgac gaccttaaac gtcagcgcgg gggcagagac ccgggcggca aacctcatct
     1021 cgggcatcct cgtcggggcg gtcatcctcg tcgcgggccc gctcgcggaa ctgataccgc
     1081 tttcggccct cgccggcatc ctgatcctca tcggggccga actcatctac cggccccacg
     1141 agatcgtctg catctgcagg tactcccgcc ccgggcgctg ggggatgatc gccaccttca
     1201 tatccaccca ggtcctgccg ctccagtaca gcatttacgt cggcgtcttt ctctcgcttg
     1261 ccatctacct catcacatcc acccgcgagg cctccgtggt ccgcctcgtc ccggtggagg
     1321 ggggaatgtt ccgggaagag tcgccacccg gctggctccc cgacggccgc ctcaccatcc
     1381 tctcgatagc gggaagcgtc tacttcgcca ccctccgggc catcgagcgg tcgctcccgt
     1441 caccggaggg aacggacggc gcagtcgtga tcctcgccct ccgggggaga gtcgaggccg
     1501 ggacggggtt tttccggatg ctggagcgct atgcccggcg ccttctcgcc cacaacggcc
     1561 ggctcatcct cgccgaggtc gatccccggt tgcttgcggg cctcgccgag accggagtaa
     1621 cggagaccgt cggtcccgca aacatattcg tcgcgacccc gaccatctgg gagtcgctca
     1681 tggaggcgat ccaaacggcg gaggccggaa catgagaccc cgcccggaaa actctttctt
     1741 tcccgggcac ccataccttt cttgtggata tgaaccccga gagcctctct ccccccgccc
     1801 gtatcgcgat cgtcggggcg gcggtcgtca tcgtcctcgc ggggatccag gcggccacac
     1861 cgatcctcgg cccattcctc atcgccgtct tctttgccat gatcaccgcg cccatcatgg
     1921 catggctgac ccgccgggga gtgccccggg ccctcgctgc cgggatggtg gtcacggggc
     1981 tcgccgtggt ccttgtcggg acgatcgcct tcctcggcat ggccttcacc gagttcctcc
     2041 tctccctgcc gcgctatgag aaagcgctcg aggcgcaggc aaccatcatc gccagctacg
     2101 gcatcgatac taccggcatc caggtctggg actacatcga cagaggattt gtgatccagc
     2161 aggcggcaga gatcgcccgg caggtcggga acatcgcctt cgatgccatc atcgtcgcga
     2221 tagcgatcgg gttcctcctc atcgaggcgc cccggttcgc ggccgccctt gcacgacgcc
     2281 tggggccgga gagtccaccc taccggcacc tcacgcaatc gggccggttc ctcatcgagt
     2341 acgtcgtgat caggaccgag gtgaacctgg tcaccggggt cgggaccggg ctcttccttg
     2401 ccctcctcgg ggtcgagttc gcggcgctct gggggttcat cgccttcatc ctgagttacg
     2461 tcccctacat aggcctcgtc gtggccgcaa tacccccgac actccttgcc ctggtcgggt
     2521 tcggtccgtc gggggccctg gccgtcatcg tcgccatcac cctcatcgac gccgcggcgg
     2581 agaacctggt cttcccccag atcacgagaa aggggctcaa cctctcgccg ttcgtcgtca
     2641 tcttctcggt cgtcttctgg ggtctcgtcc tcggggcggt cggggtcttc ctcgccatac
     2701 ccctgacgat cgcggtgaaa ctcttccttg aaagctggga tgagacccgg tggctcgggg
     2761 agatgatgaa cgccggggag cggcaaaagt aacatccccc gggtccggcc acgggcgctc
     2821 catccctgca atatcccgcg ccggggctcc tcccgctcgc gccagccccg gaccggggag
     2881 cgaccggaac ctttttctgg cgtgccccgg aagggggggc gccatgcttg agcggatgga
     2941 gacgggtgca ggagagatcg ccggattccg attcgacggt gatatgacga ccaaagacta
     3001 caacggggtg ctgataccgg cgctcgaaga ggcgggagga acccacccgg tcttccggat
     3061 cctcttctcc atcgtgaact ttcacggctg gaaaccccgc ggaaactggg aggccctcaa
     3121 ggactggccg gggatggaga aggtcgaccg gatagcgatc gtcggcggtg agaaatggga
     3181 ggagtggatg aactatctac cggggctctt tgtcgggttt gtcggcatcg acgtccgata
     3241 cttctcaagc gaacacctga acgatgccct cgactggctg cgggagccga tcgccgcaga
     3301 agaggccgct ctcaagccgg gatagcgctg ccaaaacgag agatgcgaga tcccgggaga
     3361 gcaggcaacc ttttttcatc cccggcaacc taccggtgat cagacagcga tcccatgcgc
     3421 cattacaagg acatcatatt cgaaaaaccc ctgccggagg cgatccggcg gctcggtctc
     3481 ctcccggctg atatgcactt ccatacccgc cactccgatt cgccaacccg cgtgcgggat
     3541 gcactcaaac tcgccgcccg gcgggggatc gggcttgcga tcaccgacca caaccaggtc
     3601 ggcggcgtcg tcgaggccgg gcggcagaat atacgcgttc ccctcgtacc cggtgtcgag
     3661 gtcagcgcca acgacggccc gcacatcctc ctctacttct actc
//
LOCUS       DAOSII010000177         3703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_21893_length_3703_cov_51.055373, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000177 DAOSII010000000
VERSION     DAOSII010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21893_length_3703_cov_51.055373"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLV90_09700"
     CDS             complement(<1..262)
                     /locus_tag="PLV90_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase CobQ"
                     /protein_id="HOF97427.1"
                     /translation="MSLIVLGTASHVGKSMTVTALCRALSRRGISVAPFKSQNMSLNS
                     YVTADGSEIGIAQAVQAFAAGVEPEADMNPVLLKPKGDRTSQV"
     gene            321..1967
                     /locus_tag="PLV90_09705"
     CDS             321..1967
                     /locus_tag="PLV90_09705"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOF97428.1"
                     /translation="MEFSPAARLLLERRYLLPGETPDALFARVAGAVGGPERSREFFS
                     LLSRLLFLPNSPTLMNAGTPAGQLSACFVLPIEDTLEGIFTSLAHMALIHRSGGGTGF
                     SFSRLRPRGDAVNGVVGAASGPVSFMRVFDAATGAVRQGGRRRGANMGVLAASHPDIE
                     EFVTAKRAGGLRNFNLSVGVDERFLRCLATGKDYDLTNPRDGSVSRSIDPASLWRLIA
                     ASAHASGEPGVLFLDEINRQSTTPHLGPIEATNPCGEQPLYPYESCNLGSVNLARCIK
                     NHDIDEDLLATVVRCGIDFLDAVIDANRFPLPVIREKTLATRKIGLGVMGFAETLIRL
                     GIPYESDEALRFAGKLMEGIEALARERSEELGTAKGSFPAIEGSVYTGDMRNATVTTI
                     APTGSLHLIAGTTSGIEPVFSFAYSRVIDGQQVAIVSDLVRELLPATAGGMDVAEHVR
                     RHGTVEGLPLADHTRDLFRTAVDIAPEHHVRMQAAFQRHVDNAVSKTVNLKETATAGE
                     IARIFSLARDLGCKGITVYRYRSRPDQVLSRGCDVCIVDD"
     gene            2010..2315
                     /gene="yciH"
                     /locus_tag="PLV90_09710"
     CDS             2010..2315
                     /gene="yciH"
                     /locus_tag="PLV90_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HOF97429.1"
                     /translation="MNGGICPTCGLPKELCICEEVAKEQQRISVKINRRRYGKEVTVI
                     EGLDPYDIDLDDLSKFLKAKLACGGTVKDSSIELQGNHRERVKDLLAQKGYNMENIS"
     gene            2493..2831
                     /locus_tag="PLV90_09715"
     CDS             2493..2831
                     /locus_tag="PLV90_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein E"
                     /protein_id="HOF97430.1"
                     /translation="MAMRCSFALDPDLMEQIDQFARDRAYDRNEAILELIEAGLACQS
                     DGRTATVRQQRSFEELNRLMREIEEIKTVLTELRNEVRLVHHTIETDWNNEAKGVPFQ
                     TKHPWEFWRK"
     gene            complement(2836..3096)
                     /locus_tag="PLV90_09720"
     CDS             complement(2836..3096)
                     /locus_tag="PLV90_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97431.1"
                     /translation="MSEVRRLLVARGRVQCTPHATVPVDRCGFCVHSARVVVKGREVP
                     SPARAYCSRCRDTSPVDMAKVEEIVCDDLSGEGFRSIANIIS"
     gene            complement(3089..3586)
                     /locus_tag="PLV90_09725"
     CDS             complement(3089..3586)
                     /locus_tag="PLV90_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOF97432.1"
                     /translation="MTAFECTLCGRCCMHAGGELIEIEKRLTSRDYLCRQKIVGGTFR
                     ARVEERFLDAFRDTTTRDAHPAWCPFLRPLPGERGKYVCTIHETRPLVCRSYICCTMR
                     IFAGDGREAGKVKGRRSLTTEDAALRRCWEESVVPLATDDDTAWKSDVAAVLERAGYR
                     VEVYE"
BASE COUNT          700 a         1221 c         1161 g          621 t
ORIGIN      
        1 ccacctgcga cgtccggtcc cccttcggtt tgaggaggac cgggttcata tcggcttcgg
       61 gctcgactcc ggccgcgaag gcctggaccg cctgggcgat accgatctcg ctcccgtcgg
      121 ccgtgacgta ggagttgagg ctcatgttct gcgacttgaa cggcgcgacc gatataccgc
      181 ggcgggagag cgcacggcag agcgccgtca ccgtcatgct ctttcccacg tgggatgctg
      241 ttccaaggac gataagagac attgccggca ggagggttgg acacggcgga ttattaaggg
      301 cggggatcca tatcccgggt atggagttct cccccgcggc ccgcctcctc ctcgagcgcc
      361 gctacctcct ccccggcgag acgccggacg ccctctttgc ccgggtcgcc ggagccgtcg
      421 gcggcccgga gagatcccgc gaattcttct cgctcctctc ccggctcctc ttcctcccga
      481 actccccgac cctgatgaac gccggcaccc ccgccgggca gctctcggcc tgttttgtcc
      541 tcccgatcga ggataccctg gaagggatat tcacgagcct cgcccatatg gcgctcatcc
      601 accggtccgg gggagggacc gggttctcgt tctcccggct ccggccgcgc ggagatgccg
      661 tcaacggggt cgtgggcgcc gcaagcgggc cggtctcgtt catgcgggtc tttgacgccg
      721 cgaccggcgc ggtccggcag ggcggccggc ggcggggggc aaacatgggc gtgctcgcgg
      781 catcccaccc cgatatcgag gagttcgtca ccgcaaaacg ggccggaggc ctccggaact
      841 tcaacctctc ggtcggcgtc gacgaacggt tcctccggtg ccttgcgacg gggaaggact
      901 acgacctcac gaacccccgc gacggcagcg tctcgcggag catcgatccg gcgtcgctct
      961 ggcgcctcat cgccgcgtcg gcccacgcct ccggcgagcc cggggtgctc ttcctcgacg
     1021 agatcaaccg gcagagcacc actccgcacc tcgggcccat cgaggcgacc aacccctgcg
     1081 gcgagcagcc gctctacccc tacgagagct gcaacctcgg gagcgtcaac ctcgcccgat
     1141 gcatcaaaaa ccacgacatc gacgaagacc tgcttgccac ggtcgtccgg tgcggcatcg
     1201 acttcctcga cgcggtcatc gacgcgaacc gcttccccct gcccgtgatc cgggaaaaga
     1261 cccttgccac cagaaagatc ggccttggag tcatggggtt tgccgagacg ctcatacgcc
     1321 tcggcatacc ctacgagtcg gatgaggcgc tccggtttgc cgggaagctg atggagggga
     1381 tcgaggctct cgcccgggag cgctcagagg agctcgggac ggcgaaaggc tcgtttcccg
     1441 cgatcgaggg gtccgtctac accggcgata tgcgcaacgc caccgtcacg accatcgccc
     1501 ccacgggctc gctccacctc atcgccggaa cgacgagcgg catcgaaccg gtcttctcgt
     1561 ttgcctacag ccgggtgatc gacgggcagc aggtcgctat cgtgagcgat ctcgtccggg
     1621 aactcctgcc cgcgaccgcc ggcgggatgg acgtcgcgga gcacgtccgc cggcacggga
     1681 ccgtcgaagg cctccccctt gcggaccata cccgcgacct cttcaggacg gccgtcgata
     1741 tagcgcccga acaccacgtc cggatgcagg ccgcgttcca gcggcacgtg gacaacgccg
     1801 tctccaagac ggtgaacctc aaagagaccg caaccgcggg cgagatcgcc cgcatcttct
     1861 ccctcgcccg agacctcggg tgcaagggca tcaccgtcta ccgctaccgg agccggccgg
     1921 accaggttct ctcgcgggga tgcgacgtct gtatcgtcga tgattgacca tgccaaataa
     1981 tatataacga gagctgaaag gtacatgcaa tgaacggtgg gatatgtccg acctgcggac
     2041 tgccaaaaga gttatgtatc tgtgaagaag ttgcaaagga acagcagaga atcagcgtaa
     2101 agataaacag gcggcggtac gggaaagagg tcacggtcat cgaggggctc gatccctacg
     2161 acatcgatct cgatgacctc tccaagttcc tgaaagcgaa actggcctgt ggcggcacgg
     2221 tcaaggactc ctcgatcgag ctgcagggca accatcgcga gcgggtaaag gacctgcttg
     2281 cccagaaagg atacaatatg gaaaatatca gttgaccccg ggcggttctc cacggagaac
     2341 cggggtgttt tttttgccgg gcggggaacg actgcgccgg aaacggtcgc gtccgcccgg
     2401 acggtgaggc ggccggcagg cggtgcgcct cagaaaatcg gttgcgtata accagcaaac
     2461 cttatctgga ttcacctgga gaaatggagg gtatggcgat gagatgctcg tttgcactcg
     2521 atcccgacct gatggagcag atcgatcagt ttgcgagaga tcgtgcatac gaccggaacg
     2581 aggctatact ggagttgatc gaggccggtc ttgcctgcca gagcgacggc aggacggcga
     2641 ccgtgcggca gcagcgctcg tttgaagagt tgaaccggct catgcgcgag atcgaggaga
     2701 tcaagacggt cctcaccgag ctcaggaacg aggtccgtct ggtccaccac accatcgaga
     2761 ccgactggaa caacgaggcg aagggcgtcc cgttccagac gaaacacccc tgggagttct
     2821 ggcggaagta gtccgtcagc tgatgatgtt tgcgatgctc cggaacccct ccccggagag
     2881 gtcgtcgcag acgatctcct cgaccttcgc catatcgacg ggagatgtat cacggcaccg
     2941 gctgcagtag gcccgggccg gcgacggcac ctctctccct tttaccacga cgcgggcgga
     3001 gtggacacag aacccgcacc ggtcgacggg cacggtcgcg tgcggcgtgc actgcacccg
     3061 gccccgggcg actaaaaggc gccggacctc actcataaac ctcgacccgg tagcccgcac
     3121 gctcgagcac cgcggcgaca tcgctcttcc aggcggtatc gtcgtcggtc gcgagcggta
     3181 cgaccgactc ctcccagcac cggcggaggg cggcatcctc ggtggtcagg ctccgcctcc
     3241 ccttcacctt cccggcctcc cgcccgtcgc cggcgaatat ccgcatggtg cagcagatgt
     3301 aggaccggca gaccaggggc cgggtctcgt ggatggtgca gacgtacttc cctctctccc
     3361 cgggaagggg gcgcaggaac gggcaccagg cggggtgcgc atctctcgtt gtggtatccc
     3421 tgaacgcatc gaggaaccgc tcctcgaccc gggcgcgaaa cgtgccgccg acgatcttct
     3481 gccggcagag gtagtcccgg ctcgtgagcc tcttctcgat ctcgatgagc tcgccgcccg
     3541 cgtgcatgca gcacctcccg cagagcgtgc attcaaacgc cgtcatccct gtagactctc
     3601 tttgcaacac aggataaagg ttgggagcct gagaggccat accaagaatt aaatcgatac
     3661 gcagacaacc gtgtctcatg aaggtgtgca taatgtgcgg agg
//
LOCUS       DAOSII010000178         3685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_22041_length_3685_cov_44.053444, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000178 DAOSII010000000
VERSION     DAOSII010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22041_length_3685_cov_44.053444"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..881)
                     /locus_tag="PLV90_09730"
     CDS             complement(87..881)
                     /locus_tag="PLV90_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF97433.1"
                     /translation="MPLLCARGVSFAYNERRVLEDISFTLDAGEVLGLVGPNGSGKTT
                     LIRCLDRILAPEGEIHLDGRDMLSMSRPEIARMIAYVPQNSGILSSATVFETVLMGRR
                     PHLRWSVSEEDKQTVSDALSLLGISAFASRRIDRLSGGERQRVFIARAIAQDARVLLL
                     DEPTSALDIRNRLEVMALLFRLAEERGLAVVAAIHDLNLAARYCHRLLILRDGLVLGQ
                     GTPSSLLTPGMVRAAYGIEAAVKDEVGVPYVVPLRPSDNDRPVELP"
     gene            complement(881..1945)
                     /locus_tag="PLV90_09735"
     CDS             complement(881..1945)
                     /locus_tag="PLV90_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOF97434.1"
                     /translation="MMAETGKQEYTRLTEKRVLFIVGLILILIALAGVAVTLGSANLS
                     VADSYLAILAGLFPDAVDLPDRMIESNAVGIVWGWRLHRVLFGIAAGFGLAIAGTVMQ
                     GVLRNPLASPFTLGIASAASCGASIAIVLGAGILSGSLLVIGNAFLFAMLAAAAIYGM
                     ARLRGMGSEAMILAGIALMYLFSAITSLLQYLGTAAKVQEVVFWMFGSLDKSSWPKLG
                     IVAIVIVAVIPPLLWRAWDLNALAEGDEVAASLGVPVERSMAGFMLAASLVTAVIICF
                     TGTIGFIGLVAPHITRMAIGTDHRTLLPASGLVGAVLLLGADCLARTLIPGAIIPVGI
                     MTAFLGIPFFLYLFMRGRDA"
     gene            complement(1942..3156)
                     /locus_tag="PLV90_09740"
     CDS             complement(1942..3156)
                     /locus_tag="PLV90_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOF97435.1"
                     /translation="MTRRLILLACSLCLLAGMAMAIPCDGDGDGTLTSSELAAAILDS
                     LDARQMGGAVEAPSPGDLRDAAFIYEHWDGRALTITDTSGQTTTLTRPIRRIALFNSD
                     SLEMLRSIGLEPDRIVGVSKYALEDPIFYPEYQGKANLGSVWSPDYEQAAGIRPDAVF
                     LYASISQSSCDEIEDTLSAIDPDIRFFRFDSFRPTVYADEVRTLGLLLGKREGADRFL
                     AFYGDATGSVAGVVDTIPVKDRIPVYLENWNDYKSAGKGSGYDEKIELAGGMNIFGDN
                     PVEYPVIDPEAVISKNPGVIVKIVGAGETTFGGYGDDDISSFERVHRAISDRPVWNQM
                     KAIRDGRVHIIHTDVIGGPEYFIGTAYFAKWFYPDRFPDLDPRAVHRQYLEEFQRLDY
                     DLDEHGTFVYPA"
     gene            complement(3153..3620)
                     /locus_tag="PLV90_09745"
     CDS             complement(3153..3620)
                     /locus_tag="PLV90_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97436.1"
                     /translation="MTPRRSVTLCILIVLLAAPVTAANIERIVTSSGDGLDVTLVVTG
                     IPVGGIVETLPDGCVWKETDHPADRTRLSGGKAAFAVIGEETIRYRVQGPQEAAGVFT
                     GTWEDLLTGESGTVGAAGTHGPESSKTPVEAPGFLSVAALAALAVAYRRRCGR"
BASE COUNT          686 a         1141 c         1225 g          633 t
ORIGIN      
        1 ctgccacgcc gagagatcga tccctgcctc ccgcgccgca tcggagaact gcaacagttc
       61 gctgccccag gcaactatag tcatcttcat ggtaactcca caggtctgtc gttgtcagaa
      121 ggtcggaggg gaaccacgta gggcaccccc acctcgtcct ttacggccgc ctcgataccg
      181 taggccgctc gcaccattcc cggcgtcagg agggaagacg gggtcccctg cccgagcacc
      241 agcccatctc tcagtatcag gagccggtgg cagtagcgtg cggcaaggtt caggtcgtgg
      301 atcgccgcca ccaccgcaag cccgcgctcc tcggcgagcc ggaagaggag ggccatcacc
      361 tcgagccggt tgcgtatatc aagcgcgctc gtcggctcgt cgaggaggag cacccgggcg
      421 tcctgcgcga tcgcccgcgc gatgaacacc cgctgccgct ccccgcccga gagccggtcg
      481 atcctgcggg acgcaaacgc agatatgccg aggagggaga gcgcgtcaga gaccgtctgc
      541 ttatcctctt cggagacgct ccagcgcagg tgcggccgcc tgcccatcag gacggtctca
      601 aagaccgtcg ccgaactgag aatgccgctg ttctgtggga cgtaagcgat catccgcgcg
      661 atctccggcc ggctcatcga gagcatgtcg cgcccgtcga gatggatctc cccctcagga
      721 gcgaggatcc ggtccaggca ccggatcagc gtcgtcttcc ccgatccgtt cggtccgacg
      781 agcccgagga cctcgcccgc atcgagggtg aacgatatat cctcgagcac ccggcgctcg
      841 ttgtaggcga acgagacccc gcgcgcgcag agcagcggca tcaggcatcc ctccccctca
      901 tgaagaggta gaggaagaag ggtatgccga ggaacgcggt catgataccg accgggatga
      961 tggccccggg gatcagggtg cgggcgaggc agtcggcgcc gaggaggaga acagccccca
     1021 cgagaccgga tgcagggagg agggtgcgat gatcggtccc gatcgccatc cgggtgatgt
     1081 ggggcgccac gaggccgatg aaccctatgg tgccggtgaa gcagatgatc acggccgtca
     1141 cgagcgatgc ggcgagcatg aacccggcca tggaccgctc caccgggacg ccgagactgg
     1201 ctgccacctc gtcaccctcg gcgagggcat tgagatccca cgcacgccag aggaggggtg
     1261 gtatgacggc gacgatgacg atcgccacga tcccgagttt cggccacgac gatttgtcga
     1321 gcgatccaaa catccagaag acgacctcct gcaccttggc cgccgtcccg aggtactgga
     1381 ggagggatgt gatcgccgag aagaggtaca tgagtgcgat cccggcgagg atcatagcct
     1441 cgctccccat gccccgcagg cgggccatgc cgtagatggc ggccgcggcg agcatggcaa
     1501 agaggaaggc gttgccgatg acgaggaggc tgccggagag gatgccggcg ccgaggacga
     1561 tcgcgatcga cgcgccgcat gacgcggcgg acgctatgcc gagggtgaag gggcttgcga
     1621 gcgggtttcg gaggacgccc tgcatcaccg tgccggctat agcaagcccg aaccccgccg
     1681 cgataccgaa gaggacacgg tgcagccgcc agccccacac gatgccgacg gcgtttgact
     1741 cgatcatccg gtcaggcagg tcgaccgcat cgggaaagag cccggcgagg atggcgaggt
     1801 aggagtcggc gaccgagagg ttggccgatc caagggtcac ggcgaccccg gcgagcgcta
     1861 tgaggatcag gatgaggccc acgatgaaga gcacccgctt ctcggtgagc cgcgtgtatt
     1921 cctgtttgcc ggtctcggcc atcatgccgg gtacacgaat gttccgtgtt catcgaggtc
     1981 gtagtcgagc cgctggaact cctccaggta ctgccggtgg acggcgcggg ggtcgaggtc
     2041 ggggaaacgg tcggggtaga accacttcgc aaagtatgcg gtgccgatga agtactcagg
     2101 ccccccgatg acatcggtgt ggatgatatg caccctgccg tccctgatcg ctttcatctg
     2161 gttccagacg gggcggtcgg agatcgcgcg gtgaacccgt tcgaaggacg agatgtcgtc
     2221 gtcgccgtag ccgccgaacg tcgtctcccc ggcgcccacg atcttgacga tgacgccggg
     2281 gtttttgctg atgaccgcct cgggatcgat caccggatac tcgacggggt tgtcaccgaa
     2341 gatgttcatg ccgccggcaa gttcgatctt ctcgtcgtag cccgagccct tccctgcgct
     2401 cttgtagtcg ttccagttct cgaggtagac cgggatccgg tcttttaccg ggatcgtatc
     2461 gaccaccccc gccacactgc cggtggcatc tccgtagaac gcgaggaacc gatccgcacc
     2521 ctctctcttc ccgaggagga ggccgagcgt ccgcacctca tcggcgtaga ccgtcggccg
     2581 gaagctgtcg aaccggaaga agcggatgtc ggggtcgatg gcgctgaggg tatcttcgat
     2641 ctcgtcgcag gacgattggg agatggaggc gtagaggaag accgcatcgg ggcggatgcc
     2701 ggccgcctgc tcgtagtcag gggaccagac cgaaccgaga ttcgctttgc cctggtattc
     2761 gggatagaag atcgggtcct cgagggcgta cttgctcacg cccacgattc ggtcgggttc
     2821 aaggcctatc gaccgcagca tctcgagaga atcgctgttg aagagcgcga tcctcctgat
     2881 cgggcgggtg agcgtcgtcg tctgtcccga tgtatcggtt atcgtgagtg cccggccgtc
     2941 ccagtgctcg tagatgaacg cggcgtcccg gaggtcgccg ggcgacggtg cttcgactgc
     3001 tccgcccatc tgccgggcat ccagcgagtc gaggattgct gccgcaagct cggacgacgt
     3061 cagtgtgccg tcgccgtcgc cgtcgcacgg gatcgccatc gccatccctg cgaggaggca
     3121 gagagagcat gcgagcagga tgaggcgcct ggtcatctgc cgcacctccg gcggtatgca
     3181 acggcaagcg cggcgagtgc ggccacggaa aggaagccag gggcctccac cggagttttg
     3241 ctggactcgg ggccatgggt tccggcggcc ccgacggtgc ctgactcgcc ggtcaacaga
     3301 tcttcccagg tgccggtgaa gactccagcg gcctcctgtg gtccctggac tcgatagcgt
     3361 atcgtttctt cgccgatgac ggcgaaagca gcttttccgc ccgagagccg ggtgcggtca
     3421 gcggggtgat ccgtctcttt ccatacgcat ccatcgggga gcgtctcgac gatgcccccg
     3481 accgggatgc cggttaccac gagggttaca tcaaggccgt ctcccgagga tgtgacgatc
     3541 cgctcgatgt tcgcagccgt tacgggggcc gcgagcagaa cgatcaggat acacagggtt
     3601 actgatctcc tcggtgtcat tagagaggga ttcatcacag cgtgttaaat atattatcat
     3661 ttgtatcatt tattcgaagt tattt
//
LOCUS       DAOSII010000179         3675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_22126_length_3675_cov_43.574586, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000179 DAOSII010000000
VERSION     DAOSII010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22126_length_3675_cov_43.574586"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..1216)
                     /locus_tag="PLV90_09750"
     CDS             complement(32..1216)
                     /locus_tag="PLV90_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOF97437.1"
                     /translation="MDPIIILGILLALLFNFANGLNDAANSIATIIATKALSPLQAVL
                     LAGVFNLLGPLLFTTAIAATIGRGLVDPAFLTPPMILMAMVGAVLWVLATSYLGIPVS
                     SSHALIGGLLGAGIAAAGMGAVFWPSLTAVGQVLFYGAVGAVLGAIGTSAYTLRKGEF
                     APWHLFLGGLIGVTLLIPSAIVLGFLKINGILAILIFIVISPTLGLISAFAFGILVTR
                     TFRDYPPRRLNSLFRNLQIFSGSLQAVGHGGNDAQNAMGIITAMLLAGGLISEFAVPL
                     WVILVSSLAISIGTLLGGWRVIDKMANRITRIRPYQGFAASTAAGGVLSLMNVFGVPV
                     STTHAITGAIMGVGATRGYSAVKWGVVREILIAWVLTIPAAAIVAGICFLVARGLLGG
                     IF"
     gene            complement(1222..1851)
                     /locus_tag="PLV90_09755"
     CDS             complement(1222..1851)
                     /locus_tag="PLV90_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOF97438.1"
                     /translation="MGIKEWVVPQDKVFFDLFDRMARTVVIAADLLVEFVENFENVKE
                     QCYRMKQLEHQGDDITHQIYEQLNRTFITPLEPEEISRLASALDDILDYIDGTVQQMY
                     GYGITETDASMVELAKLIQLSVIEIEKAVNSIRSIKDPGLIEERCIEVNRLENIADNV
                     LGHAIMDLFQTKDAITIIKLKDIYENLEIATDKCEDVANVLSDIAIRHT"
     gene            complement(1989..2297)
                     /locus_tag="PLV90_09760"
     CDS             complement(1989..2297)
                     /locus_tag="PLV90_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1894 domain-containing protein"
                     /protein_id="HOF97439.1"
                     /translation="MASRCVNNLGGQVLLKDVTPEQVNDYVRRHCKEYYEMPPGFVFR
                     DIRMLLKSPMLVGLQNRKKRVLLPFTKPCPGYGTMLYEITAKEADLDLIRSNLAKLSE
                     "
     gene            2552..>3675
                     /locus_tag="PLV90_09765"
     CDS             2552..>3675
                     /locus_tag="PLV90_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HOF97440.1"
                     /translation="MREPAVKKVLYWCDRCNVPLIGRTCACGTGVREISLLQPHDVRP
                     ALAADMALIRSLLAEQFGDVPLPEVVLLNKTGGADRADLVIVHGDRFGWLSFDPVARK
                     FSLDIAPEALPHILPHATRGILDIEAEPAVIAHRGRIGGKRFPLAAPVPDGTVIVSYR
                     NRFGTGIVRDGQVRVKELVPVTPRPRPNPGWDTVIEKNRYHLKNLERNAIRTVKKHMS
                     DRPCVNVSFSGGKDSAVVLHLARKAGVEKAFFIDTGIELPETVEFVESQGVEIIRKSG
                     DFFQAAEKAGPPGKDRRWCCKLLKLHPLKSYLADIGPCVTIQGNRWYESWNRADLEET
                     SQNPANPLQLNISPIRNWRALEVFLYIWWQKIPINPLYEKG"
BASE COUNT          752 a         1144 c         1124 g          655 t
ORIGIN      
        1 ccgggccggc ctctctccca ttccgccctc atcagaagat cccgccgagc aaaccccggg
       61 cgacaaggaa acagatcccg gccacgatcg ccgccgccgg tatggtcagg acccacgcga
      121 tgaggatctc gcggaccacg ccccacttga ccgccgaata cccgcgcgtt gcgccgacgc
      181 ccatgatcgc gccggttatc gcgtgggtcg tcgagaccgg aactccgaag acgttcatca
      241 gcgaaagaac tcctccggcg gcggtcgacg ccgcaaatcc ctggtagggt cggatccgcg
      301 tgatccggtt ggccatctta tcgatgaccc gccatccccc gaggagggtt ccaatggata
      361 tcgcaagcga agagaccagt atcacccaga gcgggacggc aaactcagag atcaacccgc
      421 cggcaagcag catggcggtg atgatgccca tcgcgttctg ggcatcgttg ccgccgtggc
      481 ccaccgcctg aagcgatccc gagaagatct ggaggtttct aaagaggctg ttcaggcgcc
      541 gtggagggta gtcgcgaaaa gtgcgagtga caaggatgcc gaaggcaaac gcggatatca
      601 gcccgagcgt gggggagatg acgatgaaga tcaggatggc aaggatgccg ttgatcttca
      661 aaaacccgag aacgatcgca gacgggatga gcagcgtcac accgatgagc cccccgagga
      721 agaggtgcca cggcgcaaac tcgcccttcc ggagcgtgta cgcgctcgtg ccgatcgcac
      781 cgaggactgc gccgacagcc ccgtagaaca ggacctgccc gaccgccgta agcgacggcc
      841 agaagaccgc ccccatcccg gcggccgcga tgccggcgcc gaggagcccc ccgatcaggg
      901 cgtggctgct ggatacgggg ataccgaggt acgaggttgc gaggacccag agcaccgcgc
      961 cgaccatggc catgaggatc atcggcggcg tgaggaaggc gggatcgaca agccccctcc
     1021 cgatggtcgc cgcaatcgcc gtggtgaaga gaagagggcc gaggaggttg aagaccccgg
     1081 caaggagcac cgcctgcagg ggcgacaggg ccttggtcgc tatgatggtg gcaatcgagt
     1141 tcgcggcatc gttcagaccg ttcgcgaagt tgaagagcag cgcgagcagg atgccgagaa
     1201 tgatgatcgg gtccatagcg gtcaggtgtg tctgatggct atatcgctca ggacatttgc
     1261 gacatcctca catttatcgg tcgctatctc caggttctcg tagatatcct tgagcttgat
     1321 gatggtgatc gcatctttcg tctggaagag gtccatgatg gcatggccga ggacgttgtc
     1381 cgcgatgttc tccaggcggt tcacttcaat gcaacgttcc tcgatcaggc caggatcctt
     1441 gatcgaccgt atgctgttga cggctttctc gatctcgatg acgctgagct ggatcaactt
     1501 cgcaagttcg accatcgagg cgtcggtctc ggtgatgccg tagccgtaca tctgctgcac
     1561 ggtgccgtcg atgtagtcga gaatatcgtc cagtgccgac gcaaggcggg atatctcctc
     1621 cggttcgagc ggcgtgatga acgtccggtt cagctgctcg tagatctggt gcgtgatatc
     1681 gtcgccctgg tgctcaagct gcttcatccg gtagcattgc tccttgacgt tctcaaagtt
     1741 ctcgacgaac tcgacgagca ggtcggccgc gataacgaca gttcgcgcca tcctgtcaaa
     1801 gagatcaaaa aaaaccttat cctgagggac tacccactct ttaatgccca caatgatcgc
     1861 tataaggttt tcctgtcggg ggctatatag cgatcggtca ggcaacttcc gtacgatccc
     1921 gtcctgctgc cgggcaagca ggacaggacg ttctctgcag cgtgccgggc gctgtacggc
     1981 ggcccgcatc actccgatag ctttgcgagg ttgctcctga tgagatcaag gtcggcctcc
     2041 tttgccgtga tctcgtagag catcgttccg taaccggggc agggttttgt gaacggcagc
     2101 aaaaccctct ttttccggtt ctggaggccg acgagcatgg gcgacttcaa gagcatccgg
     2161 atatcccgaa acacgaaacc cggaggcatc tcgtagtact ctttgcagtg cctgcgcacg
     2221 tagtcgttca cctgttccgg cgtcacgtcc ttgaggagga cctgtccgcc gaggttgttc
     2281 acgcatcgcg atgccatcgc atagatcatt cgcaccaata cgaataaatt ttatgctacc
     2341 gtccggggat caagccgtgg atcaccctga cgacgaggac gcgacgatcg tcggcgtggt
     2401 gcgccggggt ggaggaggtg cgagaccgtc gcccggatgc ggcgaatacc gggactgcgg
     2461 ccgtccacag ggccgatccc tctcctcacg agaaccggat ccgtatcatc ggttcactta
     2521 atgctcgcgg cggccaacta ttcaccacgt gatgcgcgaa ccggcagtaa aaaaagttct
     2581 ctactggtgc gaccggtgca acgtccccct catcgggcgc acctgcgcct gcgggaccgg
     2641 agtcagggag atatccctcc tgcagcccca tgacgtccgc ccggcccttg cagcggatat
     2701 ggccctcata cggagcctgc ttgccgaaca gttcggcgac gttcccctac cggaggtcgt
     2761 gctcctgaac aagaccggcg gcgccgacag ggccgacctc gtgatcgtgc acggcgaccg
     2821 gttcggctgg ctctcgttcg acccggtcgc ccggaagttc agcctcgata tcgcccccga
     2881 ggcgctcccg cacatactgc cgcacgcaac gcgcggtatc ctcgacatcg aggccgaacc
     2941 ggccgtgatc gcccacaggg ggcgtatcgg cgggaagcgg ttccccctcg cggcccccgt
     3001 gcccgacggg acggtcatcg tctcctacag gaaccggttc ggcaccggca tcgtcaggga
     3061 cgggcaggtc cgggtgaaag aactcgttcc cgtcaccccc cgacccaggc ccaacccggg
     3121 atgggacacg gtgatcgaga agaaccgcta ccacctgaag aaccttgagc gcaacgccat
     3181 ccgcaccgtc aagaagcaca tgagcgaccg gccgtgcgtg aacgtctcgt tctccggcgg
     3241 caaggacagc gccgtcgtcc tccaccttgc gagaaaggcc ggtgtggaga aggcgttctt
     3301 catcgacacc ggtatcgaac tgccggagac ggtggagttc gtggagtcgc agggggtcga
     3361 gatcatccga aaaagcggcg acttcttcca ggcggccgag aaggcgggac cgccgggaaa
     3421 ggaccgccgc tggtgctgta agctcttgaa actccacccc ctgaagagct acctcgccga
     3481 catcggcccc tgcgtcacca tacaggggaa ccgctggtac gagtcctgga accgtgccga
     3541 ccttgaggag acgagccaga acccggcaaa ccccctgcag ctgaacatat cgcccatccg
     3601 gaactggcgg gcgcttgagg tcttcctcta catctggtgg cagaaaatcc ccataaaccc
     3661 cctctacgag aaggg
//
LOCUS       DAOSII010000180         3662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_22209_length_3662_cov_51.136679, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000180 DAOSII010000000
VERSION     DAOSII010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22209_length_3662_cov_51.136679"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..790
                     /locus_tag="PLV90_09770"
     CDS             74..790
                     /locus_tag="PLV90_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4/precorrin-4
                     C(11)-methyltransferase"
                     /protein_id="HOF97441.1"
                     /translation="MHKVYIVGAGPGAPDLITVRGMDLLRRADVLIYAGSLVNPALVE
                     ESGAGLKLDSQKMTLEDIADAVEEHVRAGELVVRLHSGDPSLYGAIVEQMAEFSRRGI
                     EAEIVPGVSSLFAAAAALKTQLTLRDVSESLIVTRPAGATLAEDRIAEFSRLGQTMVV
                     FLGTERMEEVLSRVECPPETPAAVVYHASWPDQKVVTGTVADIAAKARAAGIERTALI
                     IIGKVVDPATSGFGRSVLYS"
     gene            787..1659
                     /gene="cbiG"
                     /locus_tag="PLV90_09775"
     CDS             787..1659
                     /gene="cbiG"
                     /locus_tag="PLV90_09775"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HOF97442.1"
                     /translation="MTGTVVVALERFLPDARRIADMLGADTIPYGPDVFREAFCRYRR
                     IVALMSAGIAVRGIAPLLADKWRDPAVVVVGPDLRYAVPVVGGHHGANDLARELARLG
                     IEPVITTATETRGRESVEGIAARSGCDIVNRDSTRAVNAAVLDADVPLYAIAGPGIVV
                     AGPGVSFLVRKGEYVVGVGCRKDVPAADVTRAVSEAFREAGVAPAEVLVYATTEKKRG
                     EAGLLAAVADLGGNLVFLDDETLNAEAAPSPSRASLIGLAGVAEPAALAVSKRKELVF
                     AKQTYGSVTVAIAR"
     gene            1628..2422
                     /gene="cobJ"
                     /locus_tag="PLV90_09780"
     CDS             1628..2422
                     /gene="cobJ"
                     /locus_tag="PLV90_09780"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HOF97443.1"
                     /translation="MGVLPLQSHDNGGKLYIVGTGPGELLQMTPRASKALEEADCIIG
                     NGFYLDLLGPMLAGKEVIRSGMGREVDRASRALALAGERVVAMVSGGDAGVYGMASIV
                     LEVAERSGTKVAVEVIPGITAAISAAALLGSPLSGDYVTMSLSDLLTPGEEIERRLDL
                     AFQMRVPVVLYNPRSRGRPHNLDAAVAIARRHLSDATPVGVVRNAYREGEERLITTLG
                     EFEDHFAFVDMHSIVFIGGEETRIWRDETDERGIITPRGYHRKYVY"
     gene            2370..3050
                     /locus_tag="PLV90_09785"
     CDS             2370..3050
                     /locus_tag="PLV90_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HOF97444.1"
                     /translation="MREESSRPGDTTGSTYTDLAAVTPEAYAIARTSRNLARERVGNV
                     TLEDRIRQRCSVAVGDFAMADLMRFSGDPVTAGLAALDRRVPIITDIRMVQAGILKRG
                     HESEILCALDYGEDIARERGITRTSAGFVALQDRVEGAIVVIGNAPSALLVVCDMVRA
                     NIRPALAIGTPVGFVNAAESKEALRTLDVPSVSNAGTRGGTPVAVAAMNEIITIFAER
                     AGYEGSGY"
     gene            3031..>3662
                     /locus_tag="PLV90_09790"
     CDS             3031..>3662
                     /locus_tag="PLV90_09790"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HOF97445.1"
                     /translation="MKDPVTDFEYPGAWVAACRSPDLLPDVRRGLAVLTASGTVLRRG
                     FTTGTTAAAACKAAVLSLAEDEVKGVGITLPCGIAVRVPVDAYRGTASCWKDAGDYPA
                     DVTAGLEFVATAVPSLSGSVQFVPGEGIGSFARDTPRHRQGTPAISAPALDCIRRSID
                     EAVEEAGLHGATVILTIPRGAEVAQKTLNPKMGVQAGISVLGTTGLVEPW"
BASE COUNT          577 a         1161 c         1309 g          615 t
ORIGIN      
        1 cagcaggtct accgggggga cgacctcccc gaggagagcg actacttctc gatcctctat
       61 gcgaggcgac tgaatgcata aggtctacat cgtgggtgcg gggccgggag cccccgatct
      121 catcaccgtc cggggcatgg acctcctccg gcgggcggac gtcctcatct atgcgggttc
      181 gctcgtgaac ccggcgctcg tggaagagtc cggtgccggg ctgaaactcg atagccagaa
      241 gatgacgctt gaagatatcg ccgatgccgt cgaggagcac gtccgtgccg gagagctcgt
      301 cgtccggctc cattcgggcg atccgtcgct gtatggcgcg atcgtcgaac agatggcgga
      361 gttctcgcgg cgcgggatcg aggccgagat cgtccccggg gtctcgtcgc tctttgcggc
      421 ggcggcggcc ttaaagaccc agctcaccct tcgcgacgtc tccgagagcc tgatcgtcac
      481 ccgcccggcg ggggcgacgc ttgcagagga ccggatagcc gagttctccc gcctcgggca
      541 gacgatggtg gtcttcctcg ggaccgagcg gatggaggag gttctctccc gggtggagtg
      601 cccgccggag accccggcgg cggtcgtcta ccacgcctcg tggcccgatc agaaggtcgt
      661 caccgggacg gtcgctgata ttgcggcaaa ggcccgggcg gccgggatcg agcggacggc
      721 gctgatcatc atcgggaagg tggtggatcc ggcaacgtcc gggttcggga ggtcggttct
      781 ctactcatga ccgggacggt tgtggtagcg ctcgagcggt tcctccccga cgctcgccgg
      841 attgcggata tgctcggcgc cgacactatc ccctacggcc ccgacgtctt ccgggaggca
      901 ttttgccgtt accgccggat cgtcgccctg atgtcggcag ggatcgccgt tcgcggtatc
      961 gcgcccctcc tcgccgacaa gtggcgggac ccggcggtgg tggtggtcgg cccggacctc
     1021 cgttacgccg tcccggtcgt cggcggccac cacggcgcaa acgaccttgc ccgggaactc
     1081 gcccgcctcg gtatcgagcc ggtgatcacg accgcgaccg agacccgcgg ccgggagtcg
     1141 gtcgaaggga tcgccgcccg atcggggtgc gatatcgtga accgcgactc cacccgggcg
     1201 gtgaacgcag cggtgctcga tgccgacgta cccctctacg cgatcgccgg ccccggcatc
     1261 gtcgtcgcgg gtccgggggt ctcgtttctc gtccggaagg gcgaatacgt cgtcggcgtc
     1321 gggtgccgga aggatgtccc ggcggccgac gtgaccagag ccgtcagcga ggccttccgg
     1381 gaggccggag ttgcccccgc agaggtcctc gtctacgcga cgaccgagaa gaagcgcggg
     1441 gaggccggtc tccttgccgc cgtcgcggac ctcggcggca acctcgtctt cttagacgac
     1501 gaaacgttga acgcggaggc ggctccgtca ccctcgcgcg cctccctgat cgggcttgcc
     1561 ggtgtcgcgg aacctgcggc gctcgcggtc tcgaaacgaa aagaactggt gtttgccaag
     1621 cagacttatg ggagtgttac cgttgcaatc gcacgataat ggaggaaaac tgtacatcgt
     1681 cggcaccggg ccgggcgaac tgctgcagat gacgccccgg gcgtcaaaag ccctcgaaga
     1741 ggccgactgc atcatcggca acgggttcta cctcgatctc ctcgggccga tgctcgccgg
     1801 aaaagaggtt atccggagcg gcatgggccg cgaggtcgac cgggcttcac gcgcgctcgc
     1861 ccttgccggg gagcgggtcg tcgcgatggt gagcggcggc gatgccggtg tctacgggat
     1921 ggcgagcatc gtgctcgagg tggcggagcg gtccggcaca aaagtcgcag tcgaggtcat
     1981 ccccggtatc acggcggcga tatcggcagc ggcgctcctc ggttcgccgc tctcggggga
     2041 ctacgtcacg atgagcctct cggatctcct gacaccggga gaggagatcg aacgaagact
     2101 cgatcttgcg tttcagatgc gggtgccggt cgtcctctac aaccctcgtt cccgggggag
     2161 gccgcacaac ctggatgcag cggtcgcgat cgcccgccgg cacctttccg acgcgacacc
     2221 cgtcggggtc gtccggaatg cctaccggga gggagaagag cggttgatca cgacgcttgg
     2281 cgagttcgaa gaccacttcg ccttcgtgga catgcactcg atcgtcttta tcggcggcga
     2341 ggagacacgg atctggaggg atgagacgga tgagagagga atcatcacgc cccggggata
     2401 ccaccggaag tacgtatact gaccttgcgg cggtcacccc cgaggcctac gcgatagcac
     2461 gcacgagccg gaaccttgcc cgggagcggg tgggaaacgt caccctcgag gaccggatac
     2521 ggcagcgatg ctcggtcgcg gtcggcgact ttgcgatggc cgatctcatg cggttttccg
     2581 gcgatccggt cacggcgggg cttgccgcac tcgatcggcg ggtgccgatc ataaccgata
     2641 tccggatggt gcaggcgggt atcctgaaac gcggacacga gagtgagatc ctctgcgccc
     2701 tcgattatgg ggaggatatc gcgcgggagc gcgggatcac ccggacgtct gcagggtttg
     2761 tcgccctgca ggaccgggtg gagggtgcga tcgtggtcat cgggaacgcc ccgtcggcgc
     2821 ttctcgtcgt ctgcgatatg gtgcgggcca acatccggcc ggcgctggcc atcgggaccc
     2881 cggtggggtt cgtgaacgcg gcggagtcaa aagaggcgct ccgtaccctc gacgtgccgt
     2941 cggtatccaa tgccggcacc cggggcggca caccggtcgc ggtggcggca atgaacgaga
     3001 tcatcacgat ctttgccgag cgtgcaggct atgaaggatc cggttactga tttcgagtat
     3061 cccggcgcgt gggtggcggc atgccggtcg cccgacctcc tccccgacgt ccggcggggc
     3121 cttgctgtcc tgacggcgtc gggaacagtg ctccgccggg ggttcacgac cgggacgacg
     3181 gcggccgcgg cctgcaaggc ggcggtcctc tcgcttgcgg aggatgaggt gaagggcgtg
     3241 ggcatcaccc tcccctgcgg catcgcggtc agggtcccgg tcgacgccta tcgcgggacc
     3301 gcgtcgtgct ggaaggatgc cggcgactac cctgcggacg tgacggcggg gcttgagttc
     3361 gtcgcgaccg cggttccctc gctctcgggg tcggtccagt tcgtccccgg cgagggtatc
     3421 gggagtttcg cccgcgacac cccccgtcat cgtcagggga cgccggccat cagcgccccg
     3481 gcgctcgact gcatccggcg gtcgatcgac gaggcggtgg aggaagcggg gctccacggg
     3541 gcgacggtca tcctcacgat tcctcgaggg gcggaggtgg cgcagaagac gctgaacccg
     3601 aagatggggg tgcaggccgg catatcggtc ctcgggacga ccgggctcgt ggagccctgg
     3661 ga
//
LOCUS       DAOSII010000181         3614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_22577_length_3614_cov_31.303737, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000181 DAOSII010000000
VERSION     DAOSII010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22577_length_3614_cov_31.303737"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PLV90_09795"
     CDS             complement(<1..682)
                     /locus_tag="PLV90_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOF97446.1"
                     /translation="MAGFAEPLWLVGLAGIPVLCLLYRRAVRERSYAALTFSRVAVAA
                     QVRSPGRLPRPHALFALALLALALILVGLADPHVPIEGMDEGVSVVLVLDTSGSMQAT
                     DYRPTRLEAAKAAAETLLSRLSRDDYAGVVIFESGATSAAYLSPDRDRVIERLRGIRG
                     RDGQTALGDGLALGVDMADAIPNRHRVVILLSDGAGNAGVFSAEEAAGYARERNVPVY
                     TVGIGSANP"
     gene            complement(683..1570)
                     /locus_tag="PLV90_09800"
     CDS             complement(683..1570)
                     /locus_tag="PLV90_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOF97447.1"
                     /translation="MSDATERADLIRQIRRIELKTATLADGSCLGQRRSPFRGSGMAL
                     ANLREYLPGDDARAIDWKASARMDRLVVREFEEERSQTYYLVLDRSGSATFGSETSKD
                     RRTLEVAASLIFAAHRQNGRVGLCIVTDRVEVFLPARPGKSQVIRLVQALVSHRPEGS
                     GTDLAAAFRLLCTRVRRKSSVIVISDFDDPGFARDLSLLRRRHEATAVRVTDPAEDAL
                     PDVGQVFLEDPETGEQVLVDTSDPAFRKAYADAVAAADRRTGGDLRSCRVPEVRVSTD
                     EPYDRPLRRFYAHTRYRGG"
     gene            complement(1573..2568)
                     /locus_tag="PLV90_09805"
     CDS             complement(1573..2568)
                     /locus_tag="PLV90_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOF97448.1"
                     /translation="MAGTDTEIAALNRRAREHAAALDAVRSGMETVIIGQQAVIDQVL
                     AAVLAGGHALLEGVPGIAKTLTISTLAHCLDCTFARIQFTPDLLPADITGTRIYDRNT
                     AAFRTIQGPVFHQIILADEINRAPPKVQSALLEAMQEGQVTIQGETYALPRPFFVLAT
                     QNPIESEGTYPLPEAQLDRFMLKIRMDYPTLDEEVAILDRFTADQPESPAPVLDAAGL
                     VALRSFVRSVYADPGVKRYAAQLVDATRHPAAYGLDLGPMIAFGASPRASLALLLCGK
                     AHAVLAGRGYVVPDDIRALAPAVLRHRVLLTFEAEADDLTPEEIVRRVLATVEVP"
     gene            3273..>3614
                     /locus_tag="PLV90_09810"
     CDS             3273..>3614
                     /locus_tag="PLV90_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HOF97449.1"
                     /translation="MRLGAMTASAILLMLAVCIVPVSAADDVFTDNNQTYAAVANYDG
                     ARFDIFGNNTYYFNFSKPTGGLNAIHITDSPSSLDGAVYTNRPASGTLYISDTSTDTQ
                     YHDDVILLFAVP"
BASE COUNT          623 a         1105 c         1266 g          620 t
ORIGIN      
        1 gcgggtttgc cgagccgatg cccacggtgt agaccgggac gttccgctcg cgggcgtagc
       61 cggcagcctc ctccgcggag aatacgccgg cgttgccggc gccgtcggag aggaggatca
      121 ccacccggtg ccggttcggt atggcgtcgg ccatgtcgac gccgagggca agcccgtcgc
      181 cgagggccgt ctggccgtcc cgcccccgga tgccgcggag ccgctcgatc acccggtcgc
      241 ggtcggggga gaggtaggcg gcgctcgtcg cgcccgactc gaatatgacg acccccgcgt
      301 agtcgtccct gctgagccgg gagagcaggg tctccgcggc ggcctttgcc gcctcgaggc
      361 gggtggggcg gtagtcggtc gcctgcatgc tcccggatgt gtccagcacc aggaccacgc
      421 tgacgccctc gtccatcccc tcgatcggga cgtgcgggtc ggcaaggccg accaggatga
      481 gggcgagcgc gaggagggca agggcgaaga gggcgtgcgg gcgcgggagc cggccggggc
      541 tccggacctg tgcggcgacg gcgacgcggg agaaggtgag cgcggcatac gaccgctccc
      601 ggacggcgcg ccggtagagg aggcagagga ccgggatgcc ggcaaggccg acgagccaga
      661 gcggttctgc aaatccggcc atctaaccgc cccggtagcg cgtgtgcgcg tagaaacggc
      721 gcaggggccg gtcgtagggc tcgtccgtgg agacccggac ctcgggaacc cggcacgagc
      781 gcaggtcgcc cccggtgcgc cggtcggcgg cggccacggc gtcggcgtag gccttccgga
      841 aggcggggtc cgacgtgtcc acgagcacct gctcgccggt ctcggggtct tcgaggaaga
      901 cctgccccac gtccgggagg gcgtcttccg cggggtccgt caccctgacg gcagtcgcct
      961 cgtgccgccg gcgcaggagc gagaggtcgc gggcgaaccc ggggtcgtcg aagtcggata
     1021 tgacgatgac cgaggatttg cgccggaccc tcgtgcagag gagacggaac gcggcggcga
     1081 ggtcggtgcc gcttccctcc ggccggtggg agacgagggc ctgcacgagc cggatgacct
     1141 ggctcttccc cggccgggcc ggcaggaaga cctcgacgcg gtcggtgacg atgcagaggc
     1201 ccacccgccc gttctgccgg tgagcggcaa atatgaggct cgcggccacc tcgagtgtcc
     1261 ggcggtcttt gctcgtttcg gacccgaacg tcgcggaccc cgaccggtcg aggacgaggt
     1321 agtaggtctg gctccgctcc tcctcgaact ccctgacgac gagccggtcc atccgtgccg
     1381 aggctttcca gtcgatcgcc cgggcgtcgt cgccggggag gtactcgcgg aggtttgcaa
     1441 gcgccatacc gctcccccgg aagggggagc gcctctggcc gaggcaggag ccgtcggcca
     1501 gtgtggcggt cttgagttct atccgccgga tctgcctgat gagatccgcc cgctccgtag
     1561 cgtcgctcac cgtcagggca cctccacggt cgcgaggacc ctccgaacga tctcttcggg
     1621 ggtgagatcg tccgcttccg cctcgaaggt gaggaggacc cggtgcctga ggacggcggg
     1681 ggcgagggcc cggatgtcgt cggggacgac gtagccccgg ccggcgagca ccgcgtgcgc
     1741 cttgccgcag aggaggaggg cgagcgacgc acgcggggat gccccgaagg cgatcatggg
     1801 gccgaggtcg aggccgtatg ccgccgggtg ccgggtggcg tcgaccagtt gcgcggcgta
     1861 gcgtttgacg cccgggtcgg cgtagaccga ccgcacgaac gaccggaggg cgacaagacc
     1921 ggcggcatcg aggaccggcg ccggggattc gggctggtcc gccgtgaacc ggtcgagtat
     1981 ggcgacctct tcgtcgagtg tcgggtagtc catccggatc ttgagcatga atcggtcgag
     2041 ctgcgcctcg gggagcgggt aggtgccctc ggactcgatc gggttctggg tggcgaggac
     2101 gaagaacggg cgcggcagcg cgtaggtctc gccctggatg gtgacctgcc cctcctgcat
     2161 cgcctcgagg agggcggact gcaccttcgg cggtgcgcgg ttgatctcgt cggcaagtat
     2221 gatctggtgg aagaccggcc cctggatcgt ccggaaggcg gccgtgttcc ggtcgtagat
     2281 ccgggtcccg gtgatgtcgg cggggaggag gtcgggggtg aactggatcc gggcgaaggt
     2341 gcagtcgagg cagtgcgcga gggtgctgat cgtgagcgtc tttgctatcc ccggcacccc
     2401 ttcgaggagg gcgtggccgc cggcaagcac cgccgcgagc acctggtcga taaccgcctg
     2461 ctgtccgatg atcaccgtct ccatgccgga gcgaaccgcg tcgagcgcgg ccgcgtgctc
     2521 ccgtgcccgg cggttgaggg ctgcaatctc cgtatccgtg cctgccatgc tgattcctgg
     2581 aagagaggtt gggggagatg ctatttgacg gttgttagtt ggggatgggt taatcagggc
     2641 agattaacga aaccggtttc gaatacggga gggctcctgc ggccgtacca cagaaaaact
     2701 ccactccata gtgatctacc ggtttcgagg atatcatagg gttttcctac ggtgacggat
     2761 gtatttatcg gcaagtacct gatggcgcga tgacacccga tccggcgctg aaaacgcgag
     2821 gccgtccggt gccctgaccc gggcttgcga tgccccttcg acaatatcat tctgcacact
     2881 acgaaaatcc ccgcaggcag gaaaggcgga gccgtatcgc aagcatccgc cacgtgccgg
     2941 tacatcgcag cgtatgcgcg atctctcatc cagcggatcg ttgtatagta taaataattg
     3001 cctttagaga tatagattgt ctccgatatt ttcccaatta ctccacatta tttcatctta
     3061 tcggcctgat cacatgaata ttcggcataa atcgatataa gaaaagtatt tataatatga
     3121 tgaccttaat gcggggtgtt gacagcggcc gtttgctcat gatcgttagt cacctttttc
     3181 gtccggcttc tgtcggcagt agcccggccg tcatgcggcg cggctacact caagggaaag
     3241 agatgattga catgcaaaac aatggaaaaa caatgagact cggtgcgatg acggcatcgg
     3301 cgatcctgct gatgcttgcc gtctgcatcg tgccggtttc cgcggccgac gatgtcttca
     3361 cggacaacaa ccagacatat gccgccgtgg cgaactatga cggagcgaga ttcgacatat
     3421 tcgggaacaa cacgtactac ttcaacttct cgaagcctac cggcggactg aacgcgatac
     3481 acatcaccga cagcccatcg agtcttgacg gcgccgtata caccaaccgg ccggcatcgg
     3541 gcacactcta catctccgac accagcacgg atacccagta ccatgacgat gtgatactgc
     3601 tgtttgccgt gccg
//
LOCUS       DAOSII010000182         3612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_22597_length_3612_cov_54.766376, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000182 DAOSII010000000
VERSION     DAOSII010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22597_length_3612_cov_54.766376"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1495
                     /locus_tag="PLV90_09815"
     CDS             <1..1495
                     /locus_tag="PLV90_09815"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOF97450.1"
                     /translation="ESIRFFDTTLRDGEQTPGVSLTPAGKLEIATHLASIGVHVIEAG
                     SAAASAGERESIRVIAEAGLAAECCTYVRALAGDIDLAADAGADSVHLVVPVSDLHIS
                     AKLRKTREQVCDMAWSAVEYAKERGLIVELSGEDASRADQAFLAEVFREGVERGADRL
                     CFCDTVGLLTPERAAAIIPPLLSAPLSIHCHDDLGFGLANTVAALRAGATCAHVTVNG
                     LGERAGNTALEELVMSLETLYGVDTGIRTEELYPLSTHVARLTGVPLATNKPIVGEMA
                     FTHESGIHAHGVMRNASTYEPLRPERVGRRRRIVIGKHSGSTAVEAALNDMGYAPDSA
                     QLAEIVARIKQIGDAGMRVTDADIMAIADTVMEIEFTPCLKLRQFTIVSGSNAVPTAS
                     VTMLVNGGEVTGAAVGTGPVDAAIRALQKSVANVGSVRLDEYRVDAITGGTDAVVDVS
                     VKLSRNGKTVTSRGARTDIIMASVEAVIAGMNRLLSEEHEDRSQDSD"
     gene            1467..3140
                     /gene="ilvB"
                     /locus_tag="PLV90_09820"
     CDS             1467..3140
                     /gene="ilvB"
                     /locus_tag="PLV90_09820"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOF97451.1"
                     /translation="MKTGARTLIEALQREGVDTIFGYPGGVVLPIYDELYDSSIRHIL
                     VRHEQAAAHAADGYARASGRVGVCLATSGPGACNLVTGIATAYMDSIPIVALTGQVPT
                     GLLGNDAFQESDITGITMPVTKHNYLVKDAGDLDRTIQEAFYIARTGRPGPVLIDLPK
                     DVTTGEVKGGGAAPGEVCLRGYQPTYQGHVRQIDKALDLVVQAERPLIYAGGGVVLSG
                     ASAELLEFAETAAIPVTTTLMGLGAVPGDHPLSLGMLGMHGTQSANYAVTECDLLLAV
                     GVRFDDRVTGRIGTFAPNAAVIHIDIDPAEIGKNKPVDVPIVGDVKAVLQAFLRRMQK
                     RGDTTNWVARTNAWKAQYPLTYREDDHLRPQYVIRELSDILKGEAIITSEVGQNQMWT
                     ALYYCFKKPRTWITSGGLGTMGYGFPAAIGAHFARPDVPVVDVAGDGSFQMNIQELGT
                     VAHYRIPVKVMILNNMYLGMVRQWQELFYDRRYSYTELPPVDFVKIAHAYGVEGMRVD
                     EKAGVREAIETALATDGPFVLDFRVEREENVFPMVPAGAAINEMIGVRQ"
     gene            3137..>3612
                     /gene="ilvN"
                     /locus_tag="PLV90_09825"
     CDS             3137..>3612
                     /gene="ilvN"
                     /locus_tag="PLV90_09825"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOF97452.1"
                     /translation="MKLHTLSVLVENRAGVLSRVAGMFSRRGFNIESLAVGTCEEPNM
                     SRITIVVNGDDGVVEQVMKQTNKLIDVIKVSDLTERENVERELALIKVATETGPARAE
                     VMQIADIFRAQVVDVGAKTLVLQVTGDTGKIDALESLLRQYGIKELVRTGKIAILRG"
BASE COUNT          688 a         1195 c         1166 g          563 t
ORIGIN      
        1 tgaatcgatc cgcttttttg acactacctt acgggacggc gaacagacac cgggtgtctc
       61 gctcacgccg gccgggaaac ttgagatcgc aacgcacctc gcgagcatcg gcgtccacgt
      121 catagaagcc ggctctgccg ctgcatccgc aggagagcgc gagtccatcc gcgtaatcgc
      181 cgaagccggg cttgccgccg agtgctgcac ctacgtccgg gctcttgccg gggatatcga
      241 cctcgccgca gacgccggcg ccgactccgt ccacctcgtc gtcccggtaa gcgatctcca
      301 catcagcgcg aagctcagaa agacccgcga gcaggtctgt gacatggcct ggagcgccgt
      361 cgagtacgcg aaagaacgcg gcctgatcgt ggaattatcc ggcgaagacg catcccgcgc
      421 cgaccaggcg ttcctcgcgg aggtattccg cgaaggagtc gagcgggggg ccgaccggct
      481 ctgcttctgc gacaccgtcg ggctcctcac gcctgagcgg gcggccgcga tcataccgcc
      541 gctcctctct gccccgctct ccatccactg ccacgacgac ctcgggtttg ggctcgcaaa
      601 caccgtcgcc gccctccgtg ccggggcaac ctgtgcccac gtcaccgtca acggcctcgg
      661 ggagcgggcg gggaacacgg ccctcgaaga gctggtgatg tcgcttgaga ccctctacgg
      721 ggtcgatacc ggcatccgga cagaagaact ctatccgctc tcgacccacg tcgcccggct
      781 gaccggggtg ccgcttgcga ccaacaaacc catcgtcggc gagatggcct tcacccacga
      841 gagcggcatc cacgcccacg gggtgatgcg gaatgcaagc acctacgaac ccctccggcc
      901 cgagcgggtg ggaaggaggc gccggatcgt catcgggaaa cactccgggt cgacggcggt
      961 cgaggccgcc ctcaacgaca tggggtatgc ccccgacagt gcgcagctcg cggagatcgt
     1021 cgcccggatc aagcagatcg gggatgccgg gatgcgggtg accgacgccg acatcatggc
     1081 gatcgccgac accgtcatgg agatcgagtt tacgccctgc ctcaaactgc gccagttcac
     1141 catcgtctcg gggagcaacg cggtcccgac cgcctcggtg acgatgctcg tcaacggcgg
     1201 cgaggtcacc ggtgccgcag tcgggaccgg gccggtggat gcggcaatcc gcgcgctcca
     1261 gaaatcggtt gcgaacgtcg gcagcgtccg gctcgatgaa tatcgcgtcg atgcgataac
     1321 gggaggcaca gatgccgtcg ttgacgtatc ggtgaaactc agccggaacg ggaagaccgt
     1381 gacatcacgg ggtgcccgga ccgacatcat catggcaagc gtcgaagcag ttatcgccgg
     1441 gatgaacaga ctactcagtg aagagcatga agaccggagc caggactctg attgaagcgc
     1501 tgcagcgtga aggggtggat accatattcg gctaccccgg cggcgtggtg ttgccgatct
     1561 acgatgaact ctacgactcg tcgatccggc atattctggt aaggcatgag caggcagcgg
     1621 cgcacgcggc cgatggctac gcgcgcgcaa gcggccgcgt aggggtatgc cttgccacat
     1681 ccggtcccgg cgcgtgcaac ctggtcacgg ggatcgcgac ggcgtacatg gactccatac
     1741 cgatcgtggc gctcaccggc caggtgccga ccggcctcct cgggaacgat gcgttccagg
     1801 agtcggacat caccggcatc acgatgccgg tcaccaagca caactacctg gtgaaagatg
     1861 ccggcgacct cgaccgcacc atccaggaag cgttctacat cgcgcggacc ggccggcccg
     1921 gcccggtgct gatcgacctc cccaaagacg tcaccacggg cgaggtgaag ggcggcgggg
     1981 ccgcacccgg tgaggtctgc ctccggggct accagcccac ctaccagggg cacgtccgcc
     2041 agatcgataa agcgctcgat ctggtcgtcc aggcagagcg cccgctcatc tacgccggcg
     2101 gcggcgtggt cctctcggga gcgtcggccg aactcctgga gttcgcggaa accgctgcga
     2161 tcccggtgac gacgaccctg atgggtcttg gcgccgtccc gggcgaccat cccctctctc
     2221 ttgggatgct cggtatgcac ggcacccagt ccgcaaacta cgcggtgacg gagtgcgacc
     2281 tcctcctcgc cgtcggcgtc cggttcgatg accgggtgac gggcaggatc gggacgttcg
     2341 cgccgaacgc tgcagtcatc cacatcgata tcgaccctgc ggagatcggg aagaacaaac
     2401 cggtcgacgt cccgatcgtg ggcgacgtga aggcggtgct tcaagcgttc ctccggcgga
     2461 tgcagaagcg cggcgacaca acgaactggg ttgcccggac gaacgcctgg aaggcacagt
     2521 acccgctcac ctaccgcgaa gacgaccacc tccgcccgca gtacgtcatc cgcgagctct
     2581 ccgatatact gaagggcgag gctatcatca cgagcgaggt cggccagaac cagatgtgga
     2641 ccgccctcta ctactgcttc aagaaacccc ggacctggat cacctccggc ggccttggca
     2701 cgatgggcta cgggttcccg gcggccatcg gcgcccactt cgcccggccg gacgtcccgg
     2761 tcgtcgatgt cgccggcgac gggagtttcc agatgaacat ccaggagctc gggacggtgg
     2821 cgcactaccg gatccccgtc aaggtcatga tcctgaacaa catgtacctc ggcatggtcc
     2881 gccagtggca ggaactcttc tacgaccgcc gctactccta caccgagctc ccgccggtcg
     2941 acttcgtcaa gatcgcacac gcctacgggg tcgagggcat gcgggtcgat gagaaggccg
     3001 gcgtccgtga ggcgatcgag accgcgcttg ccaccgacgg gccgttcgtc ctcgacttcc
     3061 gggtcgagcg ggaggagaac gtcttcccca tggtccccgc gggtgctgcg atcaacgaga
     3121 tgatcggggt gcgccaatga agctgcacac cctgagcgtt ctcgtggaga accgggcggg
     3181 agtcttaagc cgggttgccg gcatgttctc gcgccggggg ttcaacatcg agagcctcgc
     3241 cgtcggaacc tgcgaagaac cgaacatgag ccggatcacg atcgtcgtga acggcgatga
     3301 cggcgtcgtc gagcaggtga tgaaacagac gaacaaactc atcgacgtca tcaaggtctc
     3361 ggacctgacg gagcgcgaga acgtggagcg ggagctcgcc ctcatcaagg tggcgacgga
     3421 gaccggcccg gcccgcgccg aggtcatgca gatcgccgat atcttccgtg cccaggtcgt
     3481 cgacgtcggt gcaaagacgc tggtgctcca ggtgaccggg gatacgggga agatcgacgc
     3541 gctcgagagc ctcctgcgcc agtacggcat caaggaactc gtccgcacag gtaagattgc
     3601 catcctccgc gg
//
LOCUS       DAOSII010000183         3594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_22713_length_3594_cov_52.512574, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000183 DAOSII010000000
VERSION     DAOSII010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22713_length_3594_cov_52.512574"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1571)
                     /gene="dnaK"
                     /locus_tag="PLV90_09830"
     CDS             complement(<1..1571)
                     /gene="dnaK"
                     /locus_tag="PLV90_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOF97453.1"
                     /translation="MVSDKVLGIDLGTTNSCMAIMEGGRATVIANAEGGRTTPSVVAF
                     TKEGERLVGTVAKRQAITNPNRTIQSIKRKMGSSEKVAIGDKTYTPQEISAMILQKMK
                     LDAEEYLGEKIKKAVITVPAYFNDAQRQATKDAGTIAGLEVLRIINEPTASALAYGIE
                     REGEATVLVYDLGGGTFDVSILQLGDGVFEVKSTAGNNRLGGDDFDNLVVDYLIDEFK
                     KKEGADLRKDPVAMQRLRDAAENAKIELSTVQKTTINLPYITTTESGPKFLDIDLSRA
                     KFESLIADLVDSTLGPVRQALADAKLGADDIDHVLLVGGSTRVPLVQETIKKVLKKEP
                     DKGINPDECVALGAAIQAGVLTGETKDVLLLDVTPLSLGIETLGGIATKLIERNTTIP
                     TRKSQIFSTAADSQTSVEIHVMQGERALAKDNFTLGRFQLTGIPPAPRGIPQIEVTFD
                     IDANGIVHVSAKDLGTGNEQSITIKPQDSRPSETEIQRMMNDAKKFEAEDQKKREEID
                     LRNTADTAVFTAERAL"
     gene            complement(1576..2085)
                     /locus_tag="PLV90_09835"
     CDS             complement(1576..2085)
                     /locus_tag="PLV90_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOF97454.1"
                     /translation="MKEDTSEPNERQQDRSPAADAASPALEELQKAYDDLNSRHLRLA
                     ADFDNYRKRVAREIDALTTYAIEDFAVELLEVVDNFERAEKAEGAGLREGMEQIKKLL
                     MAILERHGIRPIECRNLPFDPERHEAIACIPSAAEEGIVIDEIIRGYCMQDRIIRCAK
                     VVVSKGMEE"
     gene            2387..>3594
                     /gene="purH"
                     /locus_tag="PLV90_09840"
     CDS             2387..>3594
                     /gene="purH"
                     /locus_tag="PLV90_09840"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOF97455.1"
                     /translation="MKWALLSVWDKAGIVDLAKVLTGHGYRILSSGGTGTALAKAGVP
                     YTDVATYTGFPEMMHGRVKTLHPKVHGGILGRRGIDDAVMQEHGIEPIDLVVVNLYPF
                     EAMRDAGLGIDEIVEYIDIGGPAMVRAAAKNHKYVAVVVDPADYRMVADAVRRGGFTD
                     EERLNLAAKAFARTAAYDGAIVNYLTGIGRQFPETFTVQYRGGRALRYGENPHQAAAV
                     YGDAGIAAEQPLQGKQMSYNNYLDVDAAVGLLREFDDCAVVIVKHNNPCGVATGENLL
                     ATYIAAREVDPVSAYGSIVAMNREVAADVARELTGTFVEVVVAPSFTPEALEIMKAKE
                     NMRVLRLPEPVVQDAIRSIDGGALVQRTEPFREDWQVVTEREPTAHEMRAMHLAWKVC
                     RHTKSNAIIFA"
BASE COUNT          566 a         1120 c         1156 g          752 t
ORIGIN      
        1 agggcccgct cggcggtgaa gacggcggtg tcggcggtgt ttctaaggtc gatctcctcg
       61 cgcttcttct ggtcctccgc ctcgaacttc tttgcgtcgt tcatcatccg ctggatctcg
      121 gtctcagagg ggcgggagtc ctgcggcttg atggtgatcg actgctcgtt cccggtgccg
      181 aggtcttttg ccgagacgtg gacgataccg tttgcgtcga tatcgaacgt gacctcgatc
      241 tgcgggatcc cccgcggtgc gggcgggatg cccgtgagct ggaaccggcc cagggtgaag
      301 ttgtcctttg caagcgcccg ctcgccctgc atcacgtgga tctcgaccga cgtctggctg
      361 tcggcggcgg tcgagaagat ctggctcttc cgtgtcggga tggtggtgtt gcgttcgatc
      421 agcttcgtcg cgatgccgcc gagcgtctcg atgccgagcg agagcggtgt cacgtcgagg
      481 agcaggacgt ctttggtctc gcccgtgagc acgccggcct ggatggcggc cccgagggcg
      541 acacactcgt cggggttgat gcctttgtcg ggctccttct tcaggacctt ctttatggtc
      601 tcctgcacga gcggcacccg ggtcgacccg ccgacgagga ggacgtggtc gatatcgtcg
      661 gcgcccagtt tggcgtcggc gagcgcctgc cgcaccggcc cgagggtcga gtcgacgagg
      721 tcggcgatga ggctctcgaa cttcgcgcgg gagagatcga tgtccaggaa cttgggaccg
      781 ctctcggtcg tggtgatgta ggggaggttg atagtggtct tctggacggt ggagagctcg
      841 atcttcgcgt tctcggccgc gtcccggaga cgctgcatcg cgaccgggtc tttccggagg
      901 tcggcgcctt ccttcttctt gaactcgtcg atcaggtagt cgacgaccag gttgtcgaag
      961 tcgtcgccgc cgaggcggtt gttgccggcg gttgatttga cctcgaaaac gccgtcgccg
     1021 agctggagga tggagacgtc gaacgtgccg ccgccgaggt cgtagacgag caccgtggcc
     1081 tcgccctccc tctcgatgcc gtaggcaagc gcgcttgccg tcggctcgtt gatgatccgg
     1141 aggacctcaa ggccggcgat cgtcccggca tccttcgtcg cctgccgctg ggcgtcgttg
     1201 aagtaggcgg gcaccgttat gacggccttc ttgatcttct cgcccaggta ctcctccgcg
     1261 tccagcttca tcttctggag gatcatcgcg gagatctcct gcggcgtgta ggtcttatcc
     1321 ccgatggcga ccttctcgct cgaacccatc ttcctcttga tcgactggat ggtgcggttc
     1381 gggttcgtga tcgcctgccg ttttgcgacg gtgccgacca gccgttcacc ctctttggtg
     1441 aacgctacga cggacggggt ggtcctgccg ccttcggcgt tcgcgatgac cgtcgcccgc
     1501 ccgccttcca tgatcgccat gcacgagttg gtcgtcccga gatcgatacc aagaacttta
     1561 tctgaaacca tctgtttatt cctccattcc ttttgatact acgacttttg cgcacctgat
     1621 gatcctgtcc tgcatacagt atccgcggat gatctcatcg atcacgatcc cctcctcggc
     1681 cgccgaaggg atgcaggcga tcgcctcatg ccgttcgggg tcgaatggaa ggttccggca
     1741 ctcgatgggc cggatgccgt gccgctcaag gatggccatg aggagtttct tgatctgctc
     1801 catcccctcg cggaggccgg cgccctcggc tttctccgcc cgctcgaagt tatcgacgac
     1861 ctcaagcaac tccaccgcga agtcctcgat ggcgtaggtc gtgagggcgt cgatctcccg
     1921 ggccactcgc ttccggtagt tgtcgaagtc tgctgcaagg cgaaggtgac ggctgtttaa
     1981 gtcgtcatac gccttttgca actcctcaag cgccggagat gctgcatccg ccgccgggct
     2041 gcgatcctgt tgcctctcgt ttggttcgga tgtatcctcc ttcatacgcc tcaccttctt
     2101 gaaaatgata taccgtactg gtttgagtat ttattgtaat gtagttctat atagtattat
     2161 cgtatgctgc acccattcgg ctcctgcaac ggcccggatt gacgatcggg cactcattcg
     2221 ccattattct cccctcgtta tgtcgatcgg ccggaaagaa gggcaccccc tctccggccg
     2281 caccgatgag cccgggagcg gccggtcgct cccttctccg ggcccgggcg cagccggttt
     2341 tgcgggtagc gccagatata tctttccatg ctgctcacgt gtatccatga agtgggcact
     2401 gctgtctgtc tgggacaagg caggcatcgt tgatctcgca aaggtgctga ccgggcacgg
     2461 ctaccggatc ttaagctcgg gcggcacagg gaccgcgctc gcaaaggccg gggtcccgta
     2521 taccgacgtc gcgacctaca ccgggtttcc cgagatgatg cacgggcggg tcaagaccct
     2581 ccacccgaag gtgcacgggg gcatcctcgg ccgccgcggc atcgacgacg ccgtgatgca
     2641 ggagcacggc atcgagccga tcgatctcgt ggtggtgaac ctctatccct tcgaggctat
     2701 gcgggacgcg gggctcggca tcgacgagat cgtcgagtac atcgatatcg gcggtccggc
     2761 catggtgcgg gcggcggcga agaaccataa gtacgtcgcg gtcgtggtgg accctgccga
     2821 ctaccgcatg gtggcggatg cggtccggcg gggcgggttt acggacgagg agcggttgaa
     2881 ccttgccgcg aaagcgtttg cccggacggc ggcctacgac ggtgcgatcg tgaactacct
     2941 gacaggtatc ggccggcagt tcccggagac cttcaccgtc cagtaccgtg gcggaagagc
     3001 gctccgctac ggcgagaacc cccaccaggc cgccgccgtc tacggcgacg ccggcatcgc
     3061 cgcggagcag ccgctccagg gcaaacagat gtcctacaac aactacctcg acgtcgatgc
     3121 cgcggtcggg ctgctccggg agttcgacga ctgcgccgtg gtcatcgtca agcacaacaa
     3181 cccctgcggc gtggcgacgg gggagaacct ccttgccacc tacatcgccg cccgcgaagt
     3241 cgacccggtc tcggcctacg gttcgatcgt cgcgatgaac cgcgaggtgg cggctgatgt
     3301 cgcccgcgaa ctcacgggga cgttcgtcga ggtggtggtc gccccgtcgt ttacgcccga
     3361 ggcgctcgag atcatgaagg caaaggagaa catgcgggtg ctccggctgc ccgagccggt
     3421 cgtgcaggat gccatccgga gcatcgacgg cggggccctg gtccagcgga cggagccttt
     3481 ccgcgaggac tggcaggtcg tgaccgagcg ggagcccacc gcccacgaga tgcgggcgat
     3541 gcatcttgcc tggaaggtct gccggcacac gaagagcaac gccatcatat tcgc
//
LOCUS       DAOSII010000184         3572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_22904_length_3572_cov_42.044640, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000184 DAOSII010000000
VERSION     DAOSII010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22904_length_3572_cov_42.044640"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PLV90_09845"
     CDS             complement(<1..422)
                     /locus_tag="PLV90_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOF97456.1"
                     /translation="MVQILELIRDDHDFIRNLFDELESNPDTRDIRCITLRRELPGHM
                     DAEEATLYARLRGAKPEVVRRLMADHTAIRETLARFEVIPLRDDAWLPALAGLREEIE
                     AHFTLEEEDIFEQARRYIDINTLFELGEVFPREKVAAAR"
     gene            complement(525..977)
                     /locus_tag="PLV90_09850"
     CDS             complement(525..977)
                     /locus_tag="PLV90_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOF97457.1"
                     /translation="MAQSIIDTLKEEHKMVLSELAELSEKGTSNREQKYSTMKESLLP
                     HLVGEEKVLYPRTREEPEMRDISLEGIEEHGAVKTLLSQLDSASTAEEDVWVAKMRVI
                     QENVAHHIREEEDQLFPAMQKKMSSDELSTLNTRYEDAKKAVLPVAPR"
     gene            complement(1061..1510)
                     /locus_tag="PLV90_09855"
     CDS             complement(1061..1510)
                     /locus_tag="PLV90_09855"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOF97458.1"
                     /translation="MGQNILDLLRREHAKVLAGLDELQRKGISDRADKFNRMKNELLP
                     HMAGEERVFYPELEARGLSDLMAVSRGEHKAAREIVDRLNGIPMADEDDWVRVVPELR
                     EAMRNHIEREEKMVFSEARRTMDDALLLHIGGRFEEAKGKAHLRPLR"
     gene            1626..2939
                     /locus_tag="PLV90_09860"
     CDS             1626..2939
                     /locus_tag="PLV90_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HOF97459.1"
                     /translation="MRNEEPGSPATGMKLQRELGLPAVTLSGIGIILGAGIYALLGEA
                     AGMAGNAVWLTFAIAAAIAAFSALSYAELSSIFPRAGAEFEYVSHAFGRRLAFVVGWL
                     IILSGILGAATVSLGFAGYFSELVGLPVIPSAILITLVLAGILFIGVRETARAAIVMT
                     LIEAGGIVMVILIGLPYLGRVDYFAMPQGIPGLLQASALVFFAYMGFEEIVKLSEETK
                     NPERTIPLALMIALAVSILLYILVSLAAVSVVGWEQLAASRAPFADVAAVALGPAAFT
                     LISVIALFATANTALLMMMASSRIIYGMASSYSLPPLLARVHPRTRTPWTAVVAVLLA
                     TIAFLFAGRIDFVANVTNFTLFVTFIVINASVIALRYRAPDMIRPFKVPGAVGRLPVI
                     PVVGMASSLFLLAQLGTPVLLVGGVMVVLGAGLALAGGWQPRGTA"
     gene            complement(3069..>3572)
                     /locus_tag="PLV90_09865"
     CDS             complement(3069..>3572)
                     /locus_tag="PLV90_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOF97460.1"
                     /translation="EEVYTTVINLFSRFTPPENPDLFPAEMIGMRSIFPVLARVKVHD
                     IEFEVLESLGGHLHGQVYLFCPGHGIVFTADTLINFGSLDEDRKRYNSLADFLVTSVN
                     VDSECARRERKALLELIRDLDEELSPHGRRCLVAGGHGAVSILNDGRLEAVGPIERYR
                     AGGPKAD"
BASE COUNT          575 a         1205 c         1033 g          759 t
ORIGIN      
        1 cgtgccgccg ccaccttctc ccgtggaaag acctcgccga gctcaaagag ggtgtttatg
       61 tcgatgtagc gtctcgcctg ctcgaagata tcctcttcct cgagggtaaa gtgcgcctcg
      121 atctcttccc ggaggccggc gagcgccggc agccaggcgt catcccggag agggatcacc
      181 tcgaaccggg cgagcgtctc gcggatggca gtatgatccg ccatcagccg cctgaccacc
      241 tcgggctttg cgccccgaag tcgtgcatac agggtggcct cctccgcatc catgtgcccg
      301 ggcagctcgc ggcggagcgt gatgcaccgg atgtcccgcg tatcagggtt gctctcgagt
      361 tcgtcgaaga gattccggat gaagtcgtgg tcgtcccgga tcagttcaag aatctggacc
      421 atactctgcc tccgtcggga gagggataga ggatgaggaa cttaacggtt tcgcccggcc
      481 aaaaaaaaag tgtgtacggg tgcccctgca ctcctcctgg tggttcaccg cggcgcaacc
      541 ggaagcacgg ccttctttgc gtcctcgtac cgggtgttca gggtcgagag ttcatcgctg
      601 ctcatcttct tctgcattgc aggaaagagc tgatcctcct cttcgcgaat gtggtgggcc
      661 acgttctcct ggatcaccct catctttgcc acccagacgt cctcctctgc ggtggatgcg
      721 ctatcgagct ggctgagcag tgtcttgact gcaccgtgct cctcgatccc ctcaaggctt
      781 atatcccgca tctccggttc ttctctggtc cgaggataca ggactttttc ttccccgacg
      841 aggtgcggca gaaggctctc cttcatggtg gaatatttct gctcccggtt gctcgtgccc
      901 ttctctgaga gttcagcaag ctctgagagc accatcttgt gctcctcttt cagggtatcg
      961 ataatactct gcgccattcg atcacttccc ccccattccc gggggggcca tctggtgcgg
     1021 gataggtata taaattatat cccggcccgg gactacgggg tcacctgagc ggccgcaggt
     1081 gggccttccc cttcgcctcc tcaaaccggc ccccaatatg caggaggagg gcgtcatcca
     1141 tcgtccgtcg ggcctccgaa aagaccatct tctcctcccg ctcgatatgg ttcctcatcg
     1201 cctcgcggag ctccggcacc acgcggaccc agtcatcctc gtcggccatg gggatgccgt
     1261 tcagccgatc gacgatctcc cgggcggcct tatgctcccc gcgagagacc gccatgagat
     1321 cggataaccc ccgtgcctca agctcggggt aaaaaactct ctcctcgccg gccatgtgcg
     1381 gcaggagttc gtttttcatc cggttgaact tatccgcccg gtcgctgatg ccttttctct
     1441 gcaactcgtc cagtccggca agcacctttg catgttcccg ccgtaagagg tccaggatgt
     1501 tctgccccat cgttctcccc cacgctgagt gctccccggt gtcgggaacg gataaatacc
     1561 tatgctccgc ctccgggggt cgcctgccgg atacccataa atggagcaga tgcgtatgat
     1621 ctcacatgcg aaacgaggaa ccgggctctc ctgcgaccgg gatgaagctt cagcgcgaac
     1681 tcggtctccc tgcggtcacc ctctcgggca tcgggatcat cctcggcgcc gggatatacg
     1741 cccttctcgg ggaagcggcc ggcatggccg gcaacgccgt ctggctgacg tttgccatcg
     1801 ccgccgccat agccgcgttc agcgctctca gctacgccga actctcgtcg atctttcccc
     1861 gggccggcgc cgagttcgag tacgtctcac acgcatttgg aaggaggctt gcgttcgtcg
     1921 tcgggtggct gatcatcctc tccggcatcc tcggcgccgc gaccgtctcg ctcgggttcg
     1981 ccggctactt ttcagagctc gtcggtctgc ccgtcatccc ctccgccatc ctgatcaccc
     2041 tcgtccttgc cggcatcctc tttatcgggg tcagggagac cgcccgggca gccatcgtca
     2101 tgaccctcat cgaggccggg ggcatcgtca tggtcatcct gatcggcctc ccgtacctgg
     2161 ggcgggtgga ctactttgct atgccccagg gcatcccggg cctcctgcag gcatcggcac
     2221 tcgtcttctt tgcctacatg gggtttgagg agatcgtaaa actctcggag gagacgaaaa
     2281 atcccgagcg gaccatacct ctcgccctca tgatcgcgct tgccgtctcc atcctcctct
     2341 acatcctggt ctccctcgcc gccgtctcgg tcgtcggatg ggagcagctc gccgcctccc
     2401 gggcaccctt cgccgacgtc gcggcggtgg ccctcggccc tgcggcgttc accctcatat
     2461 ccgtcatcgc cctctttgcc acggctaaca ccgccctcct catgatgatg gcctcgtcgc
     2521 ggatcatcta cgggatggcg tcctcgtact cgcttccccc cctcctcgcc cgggtgcacc
     2581 cccggacacg aacaccctgg acggccgtcg tcgccgtcct gctcgccacc atagccttcc
     2641 tctttgcggg caggatcgac ttcgtcgcga acgtcacgaa ctttacgctc tttgtgacgt
     2701 tcatcgtcat caacgcatcc gtgatcgcgc tccggtaccg tgcgcccgac atgatccggc
     2761 cattcaaggt tcctggcgcc gtcgggagac tgccggtcat accggtcgtc ggtatggcat
     2821 cgagcctctt cctcctcgcc cagctcggca ctccggtcct gctggtcggc ggggtcatgg
     2881 tcgttctcgg tgcaggtctc gcgctggcag gagggtggca gccccgcggg acagcgtaga
     2941 gaggagtgaa cggcatcggg gtttgagaga cgggtacctg aaacttcctt cgcgctcaac
     3001 gcgcaagacc gtatcgccat cggcaatggg cagctcacgt gctacaacat gatacggcgc
     3061 gcggttcgct aatcggcctt tggtcccccg gcccggtaac gctcgatcgg accgaccgct
     3121 tccagtctcc cgtcgttgag gatggagacg gcgccgtgac cgcccgcaac caggcaccgg
     3181 cggccgtgcg gcgagagttc ctcatccaga tcccggatca gctcaagaag cgccttacgt
     3241 tcccggcgtg cacactcgct gtcgacgttg accgacgtca ccaggaagtc cgcaagcgag
     3301 ttgtagcgct tccggtcctc atcgaggctc ccgaagttga tcagcgtatc ggcggtgaag
     3361 acgatcccgt gccccgggca gaagaggtag acctggccgt gcaggtgccc gcccagggac
     3421 tcgaggacct cgaactcgat gtcgtggacc ttcacgcggg cgaggacggg gaagatcgag
     3481 cgcatgccga tcatctccgc cgggaagagg tcggggttct ccggcggggt gaagcgcgag
     3541 aagaggttga tgacggtcgt gtagacctcc tc
//
LOCUS       DAOSII010000185         3557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_23013_length_3557_cov_49.581668, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000185 DAOSII010000000
VERSION     DAOSII010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23013_length_3557_cov_49.581668"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PLV90_09870"
     CDS             <1..241
                     /locus_tag="PLV90_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOF97461.1"
                     /translation="RYREAVSCSNCTAYQAVRLNIKVRDPTEFTKKRYVHTLNSTAIA
                     TSRAIRAILENYQNEDGSVTIPGPLRPYLYGEETL"
     gene            complement(291..1862)
                     /locus_tag="PLV90_09875"
     CDS             complement(291..1862)
                     /locus_tag="PLV90_09875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF97462.1"
                     /translation="MERLINQFDDGIPMQEEHDLQNRILRTLRFQPKGMTITDVAKQL
                     GVTRNSVSKHLEILQIAGKVDVRQIGNAKLFSFAQRVPISAFLCFTKNLILILDADQR
                     IVQVNDQCLRHLRRSKDDLIGRTIREAALPVVSTPEALAVIEGLEREQVIADIRYQRE
                     SDEFFYQMQVIPTTFEDGEKGCTLVLEDITERKRYTRNMEFLARTAMELVDMPAEADI
                     YRYIVDRLIELIPGARVYVESYDEVNRQFILRALADHEFRDRLRELVGRDVEGMVFPI
                     GNLYDDPYNETMASISVVREHIFRPASEATKYSLYEICLGQIPEELCEEILDRLDIGR
                     LYGVGLAWESHIYGIIGIFFPPCRELTNRRAVESFIRQASIAIARRQTEDRLHRSERR
                     LRDLIDTSPIAAALIESDGHYTYLNGAFTGLFGYTLADIPTGRDWFWLAFPDPARRNE
                     AIAAWKTDREEAGRGSPRPRTFLVRCGDGAERLIRFRPVELSDGTEYVTYEDVTEERR
                     VHGVLLGEIAGLRRA"
     gene            complement(1930..2631)
                     /locus_tag="PLV90_09880"
     CDS             complement(1930..2631)
                     /locus_tag="PLV90_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOF97463.1"
                     /translation="MSTFRIIAGREMKLALRNRSVIITALIFAVWFPVMSGVGIASGA
                     GGDAAVIAGGIAAIVLPVGVFMGYLFCADAFLREKRDGTIETLLCTPVSLRHLWEGKV
                     AGVTVPAYLTTLLSAAVTITAVSTLAGVPVAVGPLLIAHLAIVVPVWIAAAAGLIGAA
                     QLALGMRENQILGFVFIFGFIFIIIGLQGIAPGGAGISPATEGILAAAGAALLVLARF
                     IAGKVTRERIVLTIP"
     gene            complement(2628..>3557)
                     /locus_tag="PLV90_09885"
     CDS             complement(2628..>3557)
                     /locus_tag="PLV90_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF97464.1"
                     /translation="NAVEVRNLSRSFDGRDILNDVSFTVGHGEIFGYLGPNGAGKTTT
                     IRILLGLLAPGAGDVRVLGRDLAADDDARARVGVLFENNGLSDRMSAVDNLTYYAGLY
                     GVDDPAERIDDLLALVGLADRQNDPVGTFSTGMKRRLGIVRAILHRPDIVFFDEPSSG
                     LDPGAQRMVRDLILELSRREEMTVFLNSHHLDEVQRICTSVAILAGGRIRAFDSVAHL
                     TGASDRPVVTIALADRNDSARASETIARLPFVAGCETDGNSLHCTLADPGAAPDLIAA
                     LVGAGYRIEEVRRSSRSLEEIYLEYVGRTEGTP"
BASE COUNT          653 a         1106 c         1187 g          611 t
ORIGIN      
        1 gcgctaccgc gaagcggtct cctgctcgaa ctgcacggcc taccaggcgg tcaggctgaa
       61 catcaaggtg cgggacccga ccgagttcac caaaaagcgc tacgtgcata ccttaaacag
      121 caccgcgata gcgacgtcgc gggcaattcg tgcgatcctc gagaactacc agaacgagga
      181 cggctctgtc acgatacccg ggcccctcag gccctacctc tacggcgagg agacgctgta
      241 gggggaccgg caacactatc tccggcgagt ccagggcgcc gggagaacga tcacgctctt
      301 cgcagccccg ctatctcgcc gagcagcacc ccgtgtaccc ggcgctcctc ggtgacgtcc
      361 tcgtaggtga cgtactctgt cccgtccgag agctcgaccg gccggaagcg gatcagtctc
      421 tcagccccat ccccgcaccg gacgaggaac gtccgcggcc gggggctgcc tcgcccggcc
      481 tcttcccgat cggtcttcca ggcggcgatt gcctcgtttc ggcgcgcggg atcggggaac
      541 gcgagccaaa accagtctct tccggtgggg atatccgcga gggtgtagcc gaagagaccg
      601 gtgaacgctc cattgaggta ggtatagtgg ccgtcgctct cgatgagggc cgccgcgatg
      661 ggggaggtgt cgatcaggtc ccggagccgg cgctcgctcc ggtggagccg gtcctcggtc
      721 tgccgccggg cgatggcgat ggaggcctga cggatgaacg attcgacagc ccgccgattg
      781 gtaagttccc ggcagggcgg gaagaagatc ccgatgatgc catagatatg gctctcccag
      841 gccagtccga cgccatataa ccttccgata tcaagacggt caagaatttc ttcgcaaagc
      901 tcctcaggga tctgccccag gcagatttcg tataacgagt atttcgtcgc ctccgacgcg
      961 ggacggaaga tatgctcccg tactacggag attgacgcca ttgtttcgtt gtatgggtca
     1021 tcatagaggt ttccgatggg aaacaccata ccctctacat cccgtcccac gagttccctc
     1081 aaacgatccc ggaattcatg gtctgcgagc gcccgtagga taaactgccg gttgacctcg
     1141 tcataggatt caacgtacac acgtgccccg ggaatgagct cgatgagccg gtcgacgata
     1201 taccggtaga tatccgcctc cgccggcata tccacgagtt ccatcgccgt ccgggcgaga
     1261 aactccatgt tccgggtata ccgcttccgc tcggtgatat cctcgaggac gagggtacac
     1321 cctttctccc cgtcctcgaa ggtcgtcggg atgacctgca tctggtagaa gaactcatcg
     1381 ctctccctct ggtaccgaat atcggcgatc acctgttccc gttcgagccc ctcgatcacc
     1441 gcgagcgcct cgggggtgga gacgaccgga agggcggcct cccggatggt ccggccgatg
     1501 aggtcgtctt ttgaccgccg gaggtgccgg aggcactggt cattgacctg gacgatccgc
     1561 tggtcggcgt cgaggatgag gatcaggttc ttcgtgaagc agaggaaggc ggatatcggg
     1621 acacgctggg caaaggagaa gagtttcgcg ttcccgatct gccggacgtc gacttttccc
     1681 gctatctgga ggatctcaag gtgcttcgag accgagttcc gggtgacacc gagttgtttt
     1741 gccacatcgg tgatggtcat gcccttcggc tggaaccgga gagtacgaag gatgcgattc
     1801 tggagatcgt gctcctcctg catggggatg ccatcgtcga actggttaat taacctttcc
     1861 atccgccgat ggggtacccc gcgcccccgc agggtaaacg ggggcacctg atcccggcgt
     1921 tccgccccct cacgggatcg tcaggacgat ccgctctctc gtcactttcc cggcgatgaa
     1981 acgggcgagg acgaggagtg ctgctcctgc ggccgcgaga atgccctccg tcgccggcga
     2041 tatgccggct cccccgggcg cgataccctg gagcccgatg atgatgaaga tgaacccgaa
     2101 tatgaagacg aacccgagta tctggttctc ccgcatgccg agcgcgagct gggcggcgcc
     2161 gatgagaccg gccgcggcgg cgatccagac cgggacgacg atagcgaggt gggcgatgag
     2221 cagggggcct acggcgaccg gaacgccggc gagggtcgag accgccgtta tggtcaccgc
     2281 ggccgagagg agggtcgtca ggtaggccgg gacggtgacc ccggcgacct tcccctccca
     2341 gagatggcgg agggagaccg gggtgcagag gagcgtctcg atggtgccgt cccgcttctc
     2401 gcggaggaag gcatcggcgc agaagagata gcccatgaag acaccgaccg ggaggacgat
     2461 cgcggcgatg ccgcccgcga tgaccgcggc atctccaccg gcgcccgagg ctataccgac
     2521 gcccgacatg accgggaacc agacggcaaa tatgagcgcg gtgatgatga cgctccggtt
     2581 ccggagggca agtttcatct cgcggccggc gatgatgcga aacgtgctca tggggtgccc
     2641 tccgtccggc cgacgtattc gaggtagatc tcttcaagcg accgcgacga ccgccggact
     2701 tcctctatcc ggtagccggc cccgacgagg gccgcgatga ggtccggggc ggctcccggg
     2761 tcggcaaggg tgcagtggag gctgttgccg tccgtctcgc acccggcaac gaacgggagg
     2821 cgggcgatcg tttccgatgc gcgggcggag tcgtttcggt cggcaagggc gatcgtcacg
     2881 accgggcggt ccgacgcgcc ggtgaggtgc gccaccgagt cgaacgcccg tatccgcccg
     2941 ccggcaagga ttgcgaccga cgtgcagatg cgctggacct cgtcgaggtg gtgggagttt
     3001 aaaaagacgg tcatctcctc gcggcgggag agttcgagta tgaggtcgcg gaccatcctc
     3061 tgcgcgccgg ggtcgagccc ggaggacggt tcgtcgaaga agacgatatc gggccggtgg
     3121 aggatggccc ggacgatacc gagcctccgt ttcatgccgg tcgagaacgt cccgaccggg
     3181 tcgttctgcc ggtcggcaag cccgacgagc gccaggaggt cgtcgatccg ctccgcgggg
     3241 tcgtcgacgc cgtagagccc ggcgtagtac gtgaggttgt cgacggcgct catccggtcg
     3301 gaaagaccgt tgttctcgaa gagcaccccc acccgcgccc gcgcatcgtc gtcggcggca
     3361 aggtcccggc cgagaacccg gacgtcgccc gcgccggggg caaggaggcc gagcaggata
     3421 cggatggtgg tggtcttccc ggcgccgttc gggccgaggt agccgaagat ctccccgtgc
     3481 ccgacggtga acgagacgtc gtttaagata tcgcggccgt caaaggaccg cgagaggttc
     3541 cggacctcga cggcgtt
//
LOCUS       DAOSII010000186         3539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_23163_length_3539_cov_37.739667, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000186 DAOSII010000000
VERSION     DAOSII010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23163_length_3539_cov_37.739667"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(466..1902)
                     /locus_tag="PLV90_09890"
     CDS             complement(466..1902)
                     /locus_tag="PLV90_09890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOF97465.1"
                     /translation="MRGPVDAADFKTYIFPLLFFKRISDVHDEEYRAALAESGGDEEY
                     ARFPQNYRFQIPHECHWNDLRTVTTNVGQALQKAMRCIETANPDTLYGIFGDAQWTNK
                     DRLSDAMLRDLIEHFSSLELTIANLPEDELGNGYEYLIKKFADDSGHTAAEFYTNRTV
                     VHLMTEMLEPQPGETIYDPTCGSGGMLLSAIAHLRSRGLEWRNVRLYGQERNLMTSSI
                     ARMNCFLHGIEEFQIVRGDTLSAPRFVEGDRLQRFDVVLANPPYSIKQWDRSAFAADP
                     WGRNVFGTPPQGRADYAFWQHILASMDEETGRSVRNPLPARCPLPAGGGGDAAQVDRG
                     GPDRVRSGARAEPLLQLADGGVRGRLPDEKARGAAGQDPLHQRCQRGHPGTGAEFPHR
                     RAYRADRRGLPGVLERGGVCVRGDASGDPGQRWKPEHPALRCAAGGDGLRGIGTVWGS
                     IASRCIVGVAESFAEVRRSLDALFEVRR"
     gene            2328..2831
                     /locus_tag="PLV90_09895"
     CDS             2328..2831
                     /locus_tag="PLV90_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HOF97466.1"
                     /translation="MDAPAPGKRGHGMNGSTPILALVFACGLLAGFGIYAAISSSPGN
                     TALYTAGAPEPIGPYSQAVRCSDYVFTSGQIGLDPTAGTLPDTVAGETAQVMENLQAI
                     LAEGGLDFPDVVQTRIYLTDLADFETVNAVYAGYFEEPYPARSTVQVAGLPKGARVEI
                     KMVARVR"
     gene            complement(2862..>3539)
                     /locus_tag="PLV90_09900"
     CDS             complement(2862..>3539)
                     /locus_tag="PLV90_09900"
                     /inference="COORDINATES: protein motif:HMM:NF026214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase C-terminal domain-containing
                     protein"
                     /protein_id="HOF97467.1"
                     /translation="PDPVEFSSMLVEYMGGADAIIPRLREDYARGMYEEVATIAGYIV
                     FSDPTNMEARNIQADALEQLGYQSVSGSYRNAYLTAAQDLRNTKGATDEYISTQGLEH
                     VIEMLSALTPDQLYEYLAIRLNPERAGGEDIRVNIIMESPRGNTTAVVQVKNSVLHAW
                     VGAYDDNATATISGERAALTEYLLQARPLKEKLLSIATVSGDVDAFIRMMGMFDTFVP
                     DFNIVLP"
BASE COUNT          677 a         1097 c         1030 g          735 t
ORIGIN      
        1 acccgtctcc accccttccg gtcgaagagc gggaggcttg cccaaccggg attcggccgg
       61 aggtcgccgg gaaccggccc gggcgcgatc atgagcgacc tcttccacgc acggagcgcc
      121 tttcaccgtc gagagccccg aggatctgcg taacttccgt ggtgacttcc gtggtgacag
      181 aggtgttcca gaacgatctc acgcccgcct tcggcatccc ggcgatagtg ggggctggcc
      241 cgataggtat ctgacgatta caggttccgc tctgacgatt ctctgacgat tcgtccctca
      301 tctccccctg gacgtaaccg agcaagcgtc cgcggccctt cccggtgact gaccacatcg
      361 acgggccaaa attccgcgtt gccgtgtcgc gggtgacgtc cacggtgaag ccggccagtc
      421 cgggctcttt ccgcaccggg ccggcgaaga tacaccgatc gcagttcatc gacgcacctc
      481 aaagagagca tccagcgagc gccggacctc ggcgaaactc tccgccacgc cgacaatgca
      541 tcgggaagcg atgcttcccc atactgtgcc gattcctcgc agaccatcgc cgccggctgc
      601 acaacgtaga gcgggatgct caggtttcca tcgctggccc ggatctccgg aagcgtcgcc
      661 acgtacgcaa accccgcctc gttcgagaac gcccggtaag ccccgacgat ccgctcgata
      721 tgctcgtcgg tgaggaaact ctgcgcccgt tcccgggtga cctcgttgac agcgttgatg
      781 aagaggatcc tgccccgccg ctcctcgggc tttctcgtcc ggcagacgac cacgcacgcc
      841 tccatcggcg agttgtagaa gaggttcggc ccgagcccca gaacgcactc gatcaggtcc
      901 gcctcgatca acctgcgccg catctccgcc tcctcctgcc ggaagaggac accgtgcggg
      961 aagaggattg cgcaccgacc ggccggtctc ttcgtccatg ctcgccagaa tgtgctgcca
     1021 gaaggcgtag tcggcccggc cctgcggcgg cgtcccgaag acgtttcgcc cccaggggtc
     1081 ggcggcgaag gcggaccggt cccactgctt gatggagtag ggcggatttg ccaggaccac
     1141 gtcgaaccgc tgcaggcggt cgccttccac gaatctgggc gcagagaggg tgtcgccgcg
     1201 cacgatctgg aactcctcga tgccgtggag gaagcagttc atccgggcga tggaggaggt
     1261 catcaggttc cgttcctggc cgtacaggcg aacattgcgc cactccagcc cccgggaacg
     1321 gaggtgggcg atggcggaga ggagcatccc cccggagccg caggtcggat cgtagatcgt
     1381 ctcgcccggt tgcggctcca acatctcggt catgaggtgg acgacggtgc ggttggtgta
     1441 gaactcggcc gcggtatggc cggagtcgtc ggcgaacttc ttgatcaggt actcgtagcc
     1501 gttcccgagt tcgtcctcgg gcagattggc gatggtgagt tcgagcgagg agaagtgctc
     1561 gatgaggtca cggagcatag cgtcggagag ccggtctttg ttcgtccatt gcgcgtcgcc
     1621 gaagatgccg tagagcgtat ccggattcgc cgtctcgata caccgcattg ctttctgcag
     1681 agcctggccg acgtttgtcg tcaccgtgcg gagatcgttc cagtggcact catgagggat
     1741 ctggaaccga tagttctgcg ggaaccgggc atattcttca tcgcccccgg attccgcgag
     1801 agctgcccgg tactcctcat cgtggacgtc ggagatccgt ttgaagaaga gcagcgggaa
     1861 gatgtaggtc ttgaagtcgg ccgcgtcgac aggcccacgc agaatattgg cggcctccca
     1921 gagatgtgat tcaagttaag cgagtgatgt tgtcgtggtg tccatggtgc gacctcaaga
     1981 gaactgtgca aggagagaac tgtgcaagga ctgcggggag aaagccggct atctctctca
     2041 gattccgccc tatcctctct ccggtcgttg cagcgcagta agtatcaatt ttagatcggg
     2101 actatattaa cctaccattt ttctgtatcc tcgtatctct tgatttctcc aattctaaaa
     2161 ttgcaagggg ccttctcgct tcatcttccg gcctcttact ccatagcgat gaaccgaacc
     2221 cgcatagccg cgatcgtaca tcgccgccgg atctctcgca ccggttgtgc gggttttctg
     2281 gaggtcctcc ttaaaaagaa tacctgccgg cgcatcgaac acctcatatg gacgctccgg
     2341 ccccgggcaa gcgaggacac ggtatgaacg gcagcacgcc aatcctggct ctcgtattcg
     2401 catgcggtct ccttgcaggg ttcggcatat acgcagccat atcctccagc ccgggaaata
     2461 ctgctctcta cacggccggt gccccggagc cgatcgggcc gtacagccag gccgtgcggt
     2521 gcagcgacta tgtctttaca tcggggcaga tcggtcttga cccgaccgcc ggcaccctcc
     2581 ccgatactgt cgccggcgag actgcccagg tgatggagaa cctgcaggcc atacttgccg
     2641 aaggcggcct cgacttcccc gacgtcgtcc agacccggat ctacctcacc gacctcgcgg
     2701 actttgagac ggtcaacgcc gtctacgccg ggtacttcga ggagccctat cccgcacggt
     2761 cgacggtgca ggtcgccggc ctcccgaaag gggcacgggt cgagatcaag atggtcgcga
     2821 gggtgcgata acccatactg aaaagagttt gtgtattggg ttcaggggag gactatgtta
     2881 aagtccggga cgaaggtgtc gaacattccc atcatacgga taaacgcatc cacatcccct
     2941 gaaacagtag caatactcag cagcttctct tttaatggcc tggcctggag cagatactcc
     3001 gtcaacgcag ctcgttcgcc cgagatagtc gcagtagcat tgtcatcata ggctcccacc
     3061 catgcatgga gaacggagtt cttcacctga acaactgccg tggtgttgcc tctcggtgat
     3121 tccatgatga tgttaacccg gatatcttcc ccgccggctc tctccgggtt cagccggatt
     3181 gcaagatatt catagagctg gtccggggtc agcgccgata acatctctat tacatgttcc
     3241 aatccctggg tgctgatgta ttcatccgtt gcccctttag tgtttctgag atcctgcgct
     3301 gccgtcagat acgcatttct gtacgaaccg gagactgact gatatccaag ttgttccagt
     3361 gcatcggcct ggatatttct tgcctccata tttgtcgggt cggagaagac gatatagccg
     3421 gcaatcgttg ccacctcttc gtacattccc ctcgcatagt cctctctgag cctgggtatg
     3481 attgcatccg ccccgcccat atactcgacc agcatcgagg agaactcaac cgggtcggg
//
LOCUS       DAOSII010000187         3499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_23452_length_3499_cov_48.335366, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000187 DAOSII010000000
VERSION     DAOSII010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23452_length_3499_cov_48.335366"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PLV90_09905"
     CDS             complement(<1..343)
                     /locus_tag="PLV90_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOF97468.1"
                     /translation="MEIADILNITTIPLSEITLERVVLAVIVAVIGWIVVRLIISAFT
                     KALSRASRLPELIVEFLTRFFSILLYVILVLVVLATIGIDVSSMVIGLSAAIGLILGF
                     GLQDTITNLAAG"
     gene            complement(561..1253)
                     /locus_tag="PLV90_09910"
     CDS             complement(561..1253)
                     /locus_tag="PLV90_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HOF97469.1"
                     /translation="MADATQQMVKRTEYSAVNLMKDAGVDAGRWSRLPDDETIRRTVK
                     AIESQNVRVILVDTADEALRAVVDLIPEGAEVMHGTSTTLIEIGYDRVLRENRKGWRD
                     CHAIITSENDAEKRHALRRKSVTADYFLSGVQAITGSGELVGCDKTGSRTGAWPHAAT
                     HLILVSGTNKIVPTLDDALKRCREYALPLEEQRARHVYGTGSSIGKYVILEREDTDGR
                     VTLVLVREQLGY"
     gene            complement(1348..1929)
                     /locus_tag="PLV90_09915"
     CDS             complement(1348..1929)
                     /locus_tag="PLV90_09915"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOF97470.1"
                     /translation="MDPYPDGQAATMPEVIDIATIFGPVHLIWTRSQSGPKVRRIVLP
                     HDLPTSVAAFEDAPRDGELPDDSIRALIAGIRSFLQGNDVRFDIDLLDLDRCPLFQRR
                     VLLAEFGIPRGYVSTYGRIARYLDTPGAARAVGNALARNPFPLVIPCHRVLRSDGRIG
                     GFQAGLAMKRRLLEMEGVGFREDGRAIMERIWY"
     gene            2062..3018
                     /locus_tag="PLV90_09920"
     CDS             2062..3018
                     /locus_tag="PLV90_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOF97471.1"
                     /translation="MKEWHLIGMACLLILAAGCSGQGGQGNAGENQIHADPAGFVREA
                     AAFAESAGKDAALAEFGNASGRFSRGSLYIYACDYNGTLLAHPYASEAVGANRGNWTD
                     VRGLPAIRIGAATAANGGGYIAYLYPTPENGSIDEAAEETSLPKIVYVAPAGETWWVA
                     SGSYLSGAAGPGTEPYPEPVSAMIDLVERGAAYGRDHGRATAFRAISNQTGTFVDAAG
                     HYLYAYDYNGTLLAHPYLQAAVGSNLIERQDPFGMKNIRALRDTARDGGGFVVFVWPN
                     PDRGNREELKIGYVLPVDETWWVGSGVYLSEITGVDASFPSP"
     gene            complement(3188..>3499)
                     /locus_tag="PLV90_09925"
     CDS             complement(3188..>3499)
                     /locus_tag="PLV90_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97472.1"
                     /translation="MKGGTEEKKSGEPGPVTFTCYRVAPEEENAPGEGMNTYSVVVLL
                     PGGDVRHQVVWAGTPEDIGDAIRVPPGSRVIMTEMASALVWDSENVEEEEAAVEAAPT
                     P"
BASE COUNT          633 a         1111 c         1099 g          656 t
ORIGIN      
        1 ccccggcggc aaggttggtg atggtgtcct ggagaccgaa gccgaggatg agcccgatcg
       61 ccgccgaaag cccgatcacc atcgaggaga cgtcgatccc gattgttgcg aggactacga
      121 ggacgaggat cacgtagagc aggatcgaga agaaacgggt aagaaactca acgatcagct
      181 cgggcagacg cgatgcccgg gagagagcct ttgtgaaagc agagatgatc aaccgcacga
      241 cgatccaccc gatcacggcg acgatcacgg cgagaacgac tctctcaagc gtaatttcag
      301 agagcgggat ggtggtgatg ttaaggatat ctgcaatctc catatactca aacgtggcgt
      361 atcggaacag ataatactat acggcctgac ggggcgagga agaggacgtt gtactatcgt
      421 tgcgtcggca ctcccgccgc ggggccggtt ccgggaaggc atgatatccg ggcagggagc
      481 ggagccgttg caaaaggggg atgtgatagg gatgcggctg ccccgaagcg tgcccatcca
      541 gcgggcgaat acaccccggt tcagtagccg agctgctccc ggacgaggac cagggttacc
      601 ctcccgtcgg tgtcctcccg ctcgaggatc acgtacttgc cgatagaact cccggtgccg
      661 tagacgtgcc gggcccgctg ctcttcgagc ggcagggcgt actcccggca gcgcttgagc
      721 gcatcgtcaa gggtcggcac gatcttgttt gttcccgaga cgaggatgag gtgggtcgcg
      781 gcgtggggcc acgctcccgt cctgctcccg gtcttgtcgc acccgacgag ctcccccgac
      841 ccggtgatcg cctggacccc ggagaggaag tagtcggcgg tcacgctctt tctccggaga
      901 gcgtgccgct tctccgcatc gttctcggag gtgatgatcg cgtggcaatc ccgccacccc
      961 ttccggttct ccctgagcac ccggtcatac cctatctcga tgagcgtcgt cgaggtgccg
     1021 tgcatgacct cggccccttc ggggataagg tcgacgacgg cccggagcgc ctcatcggcc
     1081 gtatccacga ggatcaccct gacgttttgg ctctcgatcg ccttcaccgt cctccggatc
     1141 gtttcgtcgt cgggtagccg gctccaccgc cctgcatcca ccccggcatc cttcatcagg
     1201 ttgactgcac tgtattccgt ccttttcacc atctgctggg ttgcatctgc catccgatca
     1261 ccccagggtg ggaggatgca ctcccacata tacctctcca tggatcaggg agggtggtgg
     1321 cgaggggtgt ttactcccgc agagggttca ataccagatt cgttccatga tcgcccggcc
     1381 gtcctcccga aacccgaccc cctccatctc gagcagcctt cgtttcatgg cgagtcccgc
     1441 ctggaatccg cctattcgcc catccgatcg caggacgcgg tggcagggga tgacgagggg
     1501 gaaggggttt ctcgcgagcg cgttgccgac cgcacgcgcg gctccggggg tatcgaggta
     1561 acgggcgatg cggccgtatg tgctgacgta tccccggggg atgccgaact cggcaaggag
     1621 cacgcgccgc tggaagaggg ggcaccggtc aaggtcgagg agatcgatat cgaaccgcac
     1681 gtcgtttccc tggaggaagg atcgtattcc ggcgatcagc gcccggatac tgtcgtccgg
     1741 gagttcccca tcccggggcg catcctcgaa cgccgccacg ctggtcggca gatcgtgggg
     1801 gaggacgatc ctccgcacct tcggtcccga ttgagatcgg gtccagatga ggtgaaccgg
     1861 accgaagatc gtcgcgatat cgatgacttc cggcatcgtg gcggcctggc cgtcagggta
     1921 tggatccatg atccggatag gcggtcgccc ctatctaact tatggtcagg cccctgtggg
     1981 gatcaggtgc agggggatcc ctattttccc tatccgggag atttcgttac gcttttctgg
     2041 tatatcgccc tacgcacgga tatgaaggaa tggcacctca taggcatggc ctgcctcctg
     2101 atcctggcgg cagggtgcag cggtcagggc ggacagggga acgcaggaga gaaccagatc
     2161 cacgcggacc cggcaggttt tgtccgggag gccgccgcct tcgcggagag tgccggaaag
     2221 gatgcggccc tcgccgagtt cggcaacgct tccggccggt tctcccgggg atccctgtac
     2281 atctacgcct gcgattacaa cggaacgctt cttgcccatc cctacgcgag cgaggcggtc
     2341 ggggcgaacc gcgggaactg gaccgacgtg cggggattgc cggctatccg gataggtgca
     2401 gcaacggcgg caaacggcgg cgggtacatc gcctaccttt acccgacacc tgagaacggt
     2461 tcgatcgatg aggcggcaga agagacctct ctcccgaaga tcgtctacgt cgcccctgcc
     2521 ggcgagacct ggtgggtggc ctcaggcagt tacctctccg gtgcggccgg gcctggaacc
     2581 gaaccgtatc cggaacccgt ctctgcgatg atcgaccttg tcgagcgcgg tgccgcctat
     2641 ggccgcgatc acggcagagc gaccgcattc agagcgatct cgaaccagac cgggactttt
     2701 gtcgatgccg cggggcacta cctctacgcc tacgactaca acgggacgct tctcgcccat
     2761 ccctaccttc aggccgctgt cgggagcaac ctgatcgagc ggcaggaccc cttcgggatg
     2821 aagaacatcc gggcgctcag ggataccgcg cgcgacggag ggggattcgt cgtctttgtc
     2881 tggccgaacc cggacagggg gaatcgcgaa gagctgaaga ttggttacgt cctccccgtc
     2941 gacgagacct ggtgggtcgg ctcaggggtc tacctctccg agatcaccgg tgtggacgct
     3001 tctttcccct ccccctgaag atctcccttt tctccccggg catgcgcccc ctcccggtca
     3061 gagatccgct gctctacgac ggcaacgcat caggattcac gaaggacttc agggacaggg
     3121 ccgttgtcga gatccggaaa gcatagcctc ccgcagcagg gctcgcacgg gcagaccaaa
     3181 aaagcggtca gggggttggc gcggcctcca cggccgcctc ttcctcctcg acgttctcgc
     3241 tgtcccagac gagcgcactc gccatctcgg tcatgatcac ccgtgaaccg ggcggaaccc
     3301 ggatcgcatc gccgatgtcc tcgggggttc ctgcccagac gacctggtgc ctgacatccc
     3361 cgccgggcag cagcactacg acgctgtagg tgttcatacc ctctcccggg gcattctcct
     3421 cttccggggc gacccggtag catgtaaacg tgaccggccc cggttctccc gacttcttct
     3481 cttctgttcc acctttcat
//
LOCUS       DAOSII010000188         3473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_23669_length_3473_cov_42.682563, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000188 DAOSII010000000
VERSION     DAOSII010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23669_length_3473_cov_42.682563"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..2492
                     /locus_tag="PLV90_09930"
     CDS             192..2492
                     /locus_tag="PLV90_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Orn/Lys/Arg decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HOF97473.1"
                     /translation="MDYLEEFPVLFIDDELHSDTAEGRASREIVKELKKEDFPVIEAL
                     TARDGVHAFLSHPHASCIIIDWELTPEATDGMLTAGDVIDLIRERNPKIPIFLNTEKL
                     AISAIPLGVISRIDGYIWKLEDTPGFIAGHIKRAAKNYLADVLPPFFQGLMDYVEEYR
                     YSWHTPGHMGGVAFLKSAAGRIFYSFFGENALRADLSASVPELGSLLEHSGVVGEAER
                     KAAEVFGADRTYFVTGGTSAANKIVSLATVTGGDVVLVDRNCHKSVMHAIIMTGAVPV
                     YLIPARNEYGIIGPIYSREFRPDVIKEKIRNCPLIEDPASQTVRLAVITNSTYDGICY
                     SAERIEEQLRDMVPYLLFDEAWSGYARFHPLYAGRFGMHPTDVVGPTVFATQSTHKVL
                     AAFSQGSMLHVRQGRGPVDHPRFNEAFMMFTSTSPQYTIIASLDVATKMMAGHSGKFL
                     IEEAIEEAIVFRKKMVTVAEEIRASPLPAENYWWFTVWQPDCIMDQEAEQALPEADDA
                     LLRNHAGCWLLHPYDAWHGFDGIEEGYAMLDPIKVTILTPGIRPGGGMEDRGIPAGIV
                     TKYLRKSGIVVEKTGYYSFLVLFTLGITKGKSGTLLAELFRFKALYDQNSPLEEVFPD
                     LVRQYPTRYSGRGLADLCQEMHGYLREKSIADLVRRVYAMLPEPVMTPAEAYRRLVRG
                     EVTAVPVSELEGRTVAAMVVPYPPGIPVIMPGERCGPATRAVVDYLVSLEEFDSLFPG
                     FESEVHGADVVAGGGRRVYYIYCVTP"
     gene            complement(2502..>3473)
                     /locus_tag="PLV90_09935"
     CDS             complement(2502..>3473)
                     /locus_tag="PLV90_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF97474.1"
                     /translation="LGINVTAVYIGLSIGPFLGGYLTEFFTWRSIFLTNIPLGIYIIH
                     EVRRHLAGEWADACEERFDIVGSVFYGAMLLAVMYGFSRLPDPSGVAAIVAGVALAAI
                     FYVWETRTPAPVLNIPLLASNRAFAFSNLAALINYGATFAVGFLLSLYLQVVRGFDPG
                     TAGLILITQPIVQTIFATPAGRLSDRVEPQYIASAGMALTAVGLALLTFLTPTTPIEQ
                     ILASLVLLGFGFALFSSPNTNAIMSSVDRSLYGVASGMLATMRTTGMMFSLGFVTVIF
                     ALLIGSTQIGPDLREPFMASLHAAFTVFTILCTLGIFASLARGKVRG"
BASE COUNT          671 a         1054 c         1154 g          594 t
ORIGIN      
        1 cagccgtatc cggggtggag ccggcggaaa ccgggcccgg gcaggtacac tctcgactgg
       61 ccggcgttcc ccgtcattga gggcaccggc tccgggggat cgatatgccg ggcgccctgg
      121 ggcgttcctg ctgcccggac caaaacattc tcaagagtcc cccagagagt atcatccagg
      181 agtacgaccg catggactac ctggaggagt ttcccgttct cttcatcgac gacgaactgc
      241 actcggacac cgcggagggc cgggcatcgc gggagatcgt gaaagaattg aaaaaagagg
      301 attttcctgt catcgaggcc ctgaccgccc gtgacggggt tcacgcgttc ctctcgcacc
      361 cgcacgcaag ctgcatcatc atcgattggg aactcacccc cgaggccacg gatggcatgc
      421 tcaccgcagg agatgtcatc gatctcatcc gggagaggaa cccgaagatc ccgatcttcc
      481 tcaacactga gaaactggcg atatctgcca tccccctcgg cgtcatatcc cggatcgacg
      541 gctacatatg gaaacttgag gataccccgg ggttcatcgc cggccatatc aagcgggcgg
      601 cgaagaacta tctcgccgac gtccttccgc cgttcttcca gggactgatg gactacgtcg
      661 aggagtaccg ctactcctgg cacacgccgg ggcatatggg gggcgtcgcg ttcctcaaga
      721 gcgccgccgg ccggatcttc tacagtttct tcggtgagaa cgccctccgt gccgacctct
      781 cggcatcggt cccggaactc ggttcgctcc tcgagcactc cggcgtcgtc ggtgaggcgg
      841 aacggaaggc agcggaggtc ttcggggccg accggaccta cttcgtcacc ggcggcacgt
      901 cggcggcgaa caaaatcgtc tctctcgcga ccgtcaccgg cggcgacgtc gtcctcgtcg
      961 accggaactg ccacaaatcc gtcatgcacg cgatcatcat gaccggcgcc gtcccggtct
     1021 acctcatacc ggcccgaaac gagtacggga tcatcggacc tatctacagc cgtgagtttc
     1081 gccccgacgt catcaaggag aagatccgga actgcccgct catagaagat ccggcatcgc
     1141 agacggtaag gctcgccgtg atcacgaact ccacgtacga tgggatctgc tacagcgcag
     1201 aaaggatcga ggagcagctg cgggatatgg tcccgtatct cctcttcgac gaggcgtggt
     1261 ccggctacgc gaggttccat cccctctacg ccgggcggtt cggtatgcat ccgacagacg
     1321 ttgtcggccc gacggtcttt gccacccagt cgacccataa ggtcctcgcc gccttctcgc
     1381 agggctcgat gctccacgtc aggcagggca gagggcccgt cgaccacccg cggttcaacg
     1441 aggcgttcat gatgttcacc tccacctccc cccagtacac catcatcgcg tcgcttgacg
     1501 tcgccaccaa gatgatggcc gggcactcgg ggaagttcct catcgaggag gcgatcgagg
     1561 aggcgatcgt ctttcggaag aagatggtga cggtggcaga agagatccgg gcaagccctc
     1621 tccctgcgga gaattactgg tggttcaccg tctggcagcc cgactgcatc atggaccagg
     1681 aggcggaaca ggcacttccg gaggcggatg atgcgctcct ccggaaccat gccgggtgct
     1741 ggctcttaca cccttacgat gcatggcatg ggtttgacgg gatcgaggag gggtacgcca
     1801 tgctcgaccc catcaaggtg accatcctca ccccgggtat caggccgggc gggggcatgg
     1861 aggaccgggg gataccggcg ggcatcgtca cgaagtacct ccgcaaaagc gggatcgtcg
     1921 tcgagaagac cggatactac tcgttcctgg tcctcttcac gctcgggatc accaagggca
     1981 agtcggggac gctgcttgcg gagctcttcc ggttcaaagc tctctatgac cagaacagcc
     2041 cgcttgaaga ggtcttcccc gatctcgtgc gccagtatcc cacccggtac tcgggcaggg
     2101 ggcttgccga cctctgccag gagatgcacg gctacttgag ggagaagtcg atcgccgacc
     2161 tcgtccggcg ggtctacgcg atgttgccgg aaccggtgat gacgccggcg gaggcttacc
     2221 gccgcctggt gcgcggcgag gtgacggcgg tgcccgtgag cgaacttgag gggaggacgg
     2281 tcgcggcgat ggtcgttccc tacccgccgg gcataccggt catcatgccg ggggagcggt
     2341 gcggtccggc cacgcgcgcg gtcgtcgatt acctcgtctc tcttgaggag ttcgacagcc
     2401 tctttcccgg gttcgagagc gaggtgcacg gggcggacgt cgtcgcgggg ggcggccggc
     2461 gggtctacta catctactgc gtcactccgt gacgtcggcg ttcacccgcg gaccttgccg
     2521 cgggcgagcg aggcgaagat gcccagagtg cagaggatgg taaagacggt gaacgccgca
     2581 tggaggctcg ccatgaacgg ctcccggaga tccggcccga tctgcgttga tcctatgagg
     2641 agggcgaaga tgacggtgac gaacccgagc gagaacatca tcccggtcgt ccgcatggtg
     2701 gcgagcatcc ccgatgcgac gccgtagagg gaccggtcga ccgagctcat gatggcgttg
     2761 gtgttcgggg aggagaagag ggcgaacccg aacccgagga ggacgaggct tgcgaggatc
     2821 tgctctatcg gggttgtcgg ggtcaggaac gtgaggaggg caaggccgac ggcggtaagt
     2881 gccattcccg cggaagcgat gtactgcggt tcgacccggt cggagagccg cccggcgggg
     2941 gtggcaaaga tcgtctggac gatcggttgc gtgatcagga tgagccccgc ggtgccgggg
     3001 tcaaaccccc tgaccacctg gagatagagg gagaggagga acccgacggc aaacgtcgcc
     3061 ccgtagttga tcagggcggc gaggtttgag aaggcgaacg cccggttcga tgcgaggagc
     3121 ggtatgttga ggaccggggc cggcgtccgg gtctcccaga catagaagat agcggcaaga
     3181 gccacgccgg caacgattgc cgcaacgccg ctggggtccg ggagtcttga gaacccgtac
     3241 atcacggcaa ggagcatggc gccgtagaag accgaaccga cgatatcgaa ccgctcttcg
     3301 catgcatcgg cccactcccc ggcaaggtgg cggcggacct cgtggatgat gtagatgccg
     3361 agggggatgt ttgttaagaa gatgctccgc caggtgaaga actcggtgag gtagccgccg
     3421 aggaacggcc cgatggagag gccgatgtag acggcggtga cgttgatccc gag
//
LOCUS       DAOSII010000189         3464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_23770_length_3464_cov_57.218539, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000189 DAOSII010000000
VERSION     DAOSII010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23770_length_3464_cov_57.218539"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="PLV90_09940"
     CDS             <1..1250
                     /locus_tag="PLV90_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF97475.1"
                     /translation="ELLLTAEIFNRNEALDINDLHPRYREFFGAGSGGSTEVKRPLVV
                     SEGAIKKTLGIPDAVCQAVRANPFVGYDEFGQRLSLPSLDAAAGWFLKKSGVSRVGEN
                     PALACYFEGKDGITVSYQDVRAKIPRFEDTKEYIEAKVSRIIAGSEEMREARDLLIIS
                     APDEIEFTLDKLVCTPRQEEVIRKIAVALEHREFLKEHGIYEFGRLLFVGPPGTGKTS
                     LALAMSRALHMPVLEVRLAMVTSQYLGETSKNIDRIFDLAKRLSPCILFIDEFDFVAK
                     TRVSDDHGAMKRAVNMLLKNIDLISFVKNGVLLIGATNHPRILDEAAWRRFDEVVEFP
                     LPDQAMRQAIIEKVAATIDCNCDFADLAARTEGFSGSDIRMMMKEAVISALMEDRRRL
                     DPVDIEQGLFRVEERNVIRGTGY"
     gene            1252..1977
                     /locus_tag="PLV90_09945"
     CDS             1252..1977
                     /locus_tag="PLV90_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOF97476.1"
                     /translation="MRITLLGTGDAIGTPKVGCDCETCRAMTAAGRSRLRTSFLIEIE
                     RKHILIESSPDLRQQLLRACSPHIDAVIWSHGHYDHFIGFGEFYRVQKVPPAYAAPPV
                     MDYCSRYLHFLPFEKHPVPVYEPFDLFGVTFTFFPVNHPPVYTCGLLIERDGITVAYT
                     SDTRKDIPEASRDLLLSRDIDLLFVDGIAPEGYNIDKHMNYAEAVRFARDVGARDYRC
                     VHMSHLVPPGMPHAGYDMETFCW"
     gene            2129..2407
                     /locus_tag="PLV90_09950"
     CDS             2129..2407
                     /locus_tag="PLV90_09950"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit L"
                     /protein_id="HOF97477.1"
                     /translation="MKLKLLELTDDGARILFEGEGITYVNALAAELLNDPEVDVAQHR
                     QVFRFADPELVVTTIGGRPPLLAITDAAKRLSGYAGELLRQMEALEAA"
     gene            2414..3025
                     /locus_tag="PLV90_09955"
     CDS             2414..3025
                     /locus_tag="PLV90_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOF97478.1"
                     /translation="MTKSAEGFARIAREVFAPIYPVIAEQVLAWSGIQSGLALDLGSG
                     PGFFAAALVEKSELSVIALDTDPAMARIAQKTAAGYRDRMIPVIGDVHCMPIRDGAVS
                     LIASRGSIYFWEDRPQAFREIERVLRPGGAAFVGGSFGTAALRNDIFREMRLRNPDWD
                     RDVARRSGGATPDLLRRELVASGVAHSRVREEEAGLWVEIRKT"
     gene            complement(3030..>3464)
                     /locus_tag="PLV90_09960"
     CDS             complement(3030..>3464)
                     /locus_tag="PLV90_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845002.1"
                     /note="eIF-2B; functions in the early steps of protein
                     synthesis by forming a ternary complex with GTP and
                     initiator tRNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit beta"
                     /protein_id="HOF97479.1"
                     /translation="HLLGELGTAGKVEGTRAVFSGKFEQEQINTIIRGYVEDYVICSE
                     CGKPDTRLVKSDRILMLRCDACGGHRPVRKRKAVADPGAAAKPTEGAIMDVTPQFLSK
                     RGDGVVKIDRYTMYVANAKPGQTVKVKITRIAGTIIFTERVD"
BASE COUNT          636 a         1112 c         1072 g          644 t
ORIGIN      
        1 tggaactcct cctcaccgcg gagatattca accggaacga ggctctcgac atcaacgacc
       61 tccacccgcg ctaccgggag ttcttcggcg ccgggagcgg cgggagcacc gaggtgaagc
      121 gtcccctggt cgtgagcgag ggggcgatca agaagacgct cggcatcccg gatgccgtct
      181 gccaggcagt tcgcgcaaac ccgttcgtcg gctacgacga gttcgggcag cgcctgagcc
      241 tcccttcgct cgatgcagcg gctggatggt tcctcaagaa aagcggggtc tcgagggtcg
      301 gggagaaccc cgcccttgca tgctattttg aaggaaagga tggtatcacc gtcagttacc
      361 aggatgtccg ggcaaaaatt ccccggtttg aggatacaaa ggagtatata gaggcgaaag
      421 tctcccggat catcgccggg agcgaggaga tgcgggaggc gcgcgacctc ctcatcatca
      481 gtgcacccga cgagatcgag ttcacgctcg ataaactggt ctgcacccca aggcaggagg
      541 aggtcatccg gaagatcgct gtcgccctcg agcaccggga gttcttaaaa gagcacggca
      601 tctacgagtt cggccggctc ctctttgtcg gcccgcccgg taccgggaag acatcgcttg
      661 ctcttgccat gtcgcgggca ctccacatgc cggtgctcga ggtccgtctc gctatggtca
      721 cctcccagta cctgggcgag acgtcaaaga acatcgaccg gatatttgac cttgcaaaga
      781 ggctctctcc ctgcattctc ttcatcgacg agttcgactt cgtcgcaaag acccgggtca
      841 gcgacgacca cggtgcgatg aagcgtgcgg tgaatatgct cctgaagaac atcgacctga
      901 tcagtttcgt caagaacggc gtcctcctca tcggggcgac gaaccatccc cgcatcctcg
      961 acgaggcagc gtggcggcgg tttgacgaag tggtggagtt cccgctcccc gatcaggcga
     1021 tgcggcaggc gatcatcgag aaggtcgcgg ctacgatcga ctgcaactgc gacttcgcgg
     1081 acctcgccgc ccggacggag gggttctcgg gctctgatat acggatgatg atgaaggagg
     1141 cagtgatatc ggcgctgatg gaggaccgcc gccgcctcga tccggtggat atcgagcagg
     1201 gcctcttcag ggtcgaggaa cgcaatgtca tccgcggaac cggatactga tatgcggata
     1261 acgctgctcg ggaccggcga tgccatcggg acgccgaagg tgggttgcga ctgcgagacc
     1321 tgccgggcga tgacggcggc ggggaggtca aggctccgga cgtcgtttct catcgagatt
     1381 gagagaaaac acatccttat cgagtcgtcg ccggacctgc gccagcagct cctccgggct
     1441 tgctcccccc atatcgacgc ggtcatctgg tcgcacggcc actacgacca cttcatcggg
     1501 tttggtgagt tttaccgggt gcagaaggtg ccgccggcct acgcggcgcc cccggtgatg
     1561 gattactgct cccgctacct ccacttcctc ccgtttgaga agcatccggt cccggtctac
     1621 gaaccgttcg acctcttcgg cgtgaccttc acgttctttc ccgtgaacca cccgccggtc
     1681 tacacctgcg gcctcctgat cgagcgcgac ggcatcacgg tcgcttacac atcggatacg
     1741 cggaaggata taccggaggc gagccgggac ctcctcctca gccgcgatat cgacctcctc
     1801 ttcgtggacg gcatcgctcc cgagggttac aatatcgata agcacatgaa ctacgccgag
     1861 gcggtccggt tcgcccgcga cgtcggggcg agggattacc gttgcgtcca catgagccac
     1921 ctggtgccgc cggggatgcc gcacgccggt tacgatatgg agacgttctg ctggtgatca
     1981 tccggcatcc ccgaacgaac gaggcgcagg gttatccgcc cccttcctcc gcccccgcac
     2041 cgctccgcgt gatgcggtcc gagctgcggc agatcgtcat ccatacctat ataccccgcc
     2101 cgaacccaaa catagggtgc gtatggccat gaaactcaaa ctcctggagc tgaccgatga
     2161 tggggcccgg atactctttg aaggcgaagg catcacctat gtcaacgcgc tcgccgccga
     2221 actcctcaac gatcccgagg tggacgtggc gcagcaccgg caggtgttcc ggttcgcaga
     2281 ccccgaactg gtcgtcacca cgatcggcgg ccggcctcct ctccttgcaa tcaccgatgc
     2341 ggcaaaacgg ctctccggct acgccggcga gctcctccgg cagatggaag cccttgaggc
     2401 cgcgtagatc ctcatgacga agagcgccga aggctttgcc cggatcgccc gggaagtctt
     2461 tgcccccatc tacccggtca tcgcagagca ggtgctcgcg tggtcgggta tacagagtgg
     2521 tctcgcgctc gacctcggga gcggccccgg cttctttgcc gccgcccttg tggagaagag
     2581 cgaactctcc gtcatcgcgc tcgataccga tccggcgatg gcccggatcg ctcaaaagac
     2641 cgctgccggc tacagagacc ggatgatccc ggtcatcggc gacgtccact gcatgccgat
     2701 ccgcgacggc gccgtctcgc tcatcgccag ccgcggttcg atctacttct gggaagaccg
     2761 gccgcaggcg ttccgcgaga tcgagcgggt tctccggccc ggcggtgccg cgttcgtcgg
     2821 cgggagcttc gggactgccg cgctccggaa cgatatattt cgcgagatgc gcctgcgaaa
     2881 ccccgactgg gaccgcgacg ttgcccggag gagcggggga gcaacccctg acctgctccg
     2941 ccgggaactt gtggcgagcg gggtcgccca ctcccgcgtc cgggaagaag aagcaggatt
     3001 gtgggtagag atcaggaaga cctgatctct taatcaacac gctcggtgaa tatgatcgtc
     3061 ccggcgatcc gggtgatctt caccttcact gtctgacccg gtttggcatt cgcaacgtac
     3121 atcgtgtagc ggtcgatctt caccacgccg tctccgcgct tcgagaggaa ctgcggggtg
     3181 acgtccatga tggcgccctc ggtcggtttt gctgccgcac cggggtctgc aaccgctttc
     3241 cgcttcctga ccggccggtg gccgccgcag gcgtcgcacc ggagcatcag aatccggtcg
     3301 ctcttgacga ggcgggtgtc gggcttaccg cactccgagc agatgacgta gtcctcgacg
     3361 tagccgcgga tgatcgtatt gatctgctcc tgctcgaact tcccggagaa gacggcccgc
     3421 gtcccctcga ccttgccggc ggtgccgagc tcgccgagga ggtg
//
LOCUS       DAOSII010000190         3457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_23818_length_3457_cov_49.727219, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000190 DAOSII010000000
VERSION     DAOSII010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23818_length_3457_cov_49.727219"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..788
                     /locus_tag="PLV90_09965"
     CDS             135..788
                     /locus_tag="PLV90_09965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOF97480.1"
                     /translation="MRERIKETDQGIQGEVDVRNFDRMQRHLRDRGLIATDEIIRSGI
                     AGGLALELGPGPGYLGLEWLQRTDETSLKAVEISRHMIDVARKNAREYGLAARVEYTL
                     GRVEAIPFADAAFDAVFSNGSLHEWSKPERAFHEIHRILKPGGRWYITDLKRNMNPAV
                     IWFMKVSVRPAEMRPGLQTSIDAAYTRSELETILAGTPLESSSLSENPFGLRVLGVK"
     gene            complement(818..1048)
                     /locus_tag="PLV90_09970"
     CDS             complement(818..1048)
                     /locus_tag="PLV90_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97481.1"
                     /translation="MAEERRRRQDEQPDEEIPFEDKGTDIVYSPVPAELGNEGRDPSR
                     PPGAPKTPTPRAEHERSKKGEERIAREQGGGE"
     gene            complement(1100..2245)
                     /locus_tag="PLV90_09975"
     CDS             complement(1100..2245)
                     /locus_tag="PLV90_09975"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HOF97482.1"
                     /translation="MRRTALMIVLAAVLAPMVAGGGVSMGSAVNDVAISADGRYAVAG
                     TDGGMIACLRQDGSVAWDTSAGDPVTAVAVAGARGYVAAGTNAGDVILLDGASGNRYW
                     TERVAGPVRDLAFSGDGRSLAVGSSRVTLFDHQGKEGWNFSTGAGATVSSIAFTADGN
                     YIAAGSDNGVIHLIGSRGNLLWQGIAYGPVTAVDASRDGTTVAAGGRDRVLQVYGRSG
                     GLPWVVQAGAPILALALPADGRFVIIGKQGGDLDCYRPREGLIWQNHTESGVLAVDVS
                     EQGEVVAAGTAGGTLRLLSGDGGVLWSFDAGAPVTSVALSANGGYIAAGAGERVFVFR
                     TADVPVEITTTPAEKATPAATPTRAAGAGALIGAGAVAVAGGWLRRR"
     gene            2349..2828
                     /locus_tag="PLV90_09980"
     CDS             2349..2828
                     /locus_tag="PLV90_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOF97483.1"
                     /translation="MSITIKRAEEAVARFMQGYNCAQSVSSVFSHDAGVPEETILRAA
                     TGFGGGMGRTGGACGAVSGAVLVIGLMVGSAGPDEKEAKDLTYDLTREFVEQFAERTG
                     SVSCTELLGCDLSTDEGRARARRDNLTRTLCPRYIRNAVDILEEVLAPVISGRPAGR"
     gene            complement(2800..>3457)
                     /locus_tag="PLV90_09985"
     CDS             complement(2800..>3457)
                     /locus_tag="PLV90_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOF97484.1"
                     /translation="NPWTYVNGSLWTIPVEVAMYGVVALLGIAGLLRRWGAIPALAVV
                     NILVWIYWFDDPRMAKVRFTLYFLIGAYLYLHRERITYRPAVAGALLLLLGLSTLTPY
                     PTVAGVVCIPYLTIYAAHLPIPYLNTFGRSGDFSYGIYIYHYPVQQTLILVTANALLL
                     PALFGLSFAVTFALAFLSWNLVEKRALTAKNLGTGEIARMLRLPSLPGPLTGRRAARK
                     "
BASE COUNT          616 a         1112 c         1143 g          586 t
ORIGIN      
        1 ttcggtcggc gcagggggat tatctgttct ttcgtccgtc gcgataccgg cagatttttt
       61 gcccccggcc atctgacctc actacgtcca gggcagaagg agatccggcc cggagcagga
      121 gagacagagt gcgcatgaga gagaggatca aagagaccga tcagggaatc cagggggagg
      181 tcgacgtccg gaatttcgac cggatgcaga ggcatctgcg ggatagaggc ctgattgcga
      241 ccgacgagat catccggtcc gggatcgccg gcggccttgc gctcgaactc ggcccggggc
      301 ccgggtacct gggccttgag tggctccaga gaacagacga aaccagcctg aaggcggttg
      361 agatcagccg gcacatgatc gatgtcgccc gaaaaaacgc ccgggaatac ggccttgcag
      421 caagggtcga atacacgctc ggccgggtcg aggcaatccc gtttgcggat gcggcgttcg
      481 atgcggtctt ctcaaatggt tccttacacg aatggagcaa acctgagagg gcgtttcacg
      541 agattcatcg catcctgaag cccggcggca ggtggtatat caccgacctg aagagaaaca
      601 tgaaccccgc cgtgatctgg ttcatgaagg tcagcgtcag gccggccgag atgcggccgg
      661 ggctgcagac atcgatcgat gccgcataca cccgaagcga gcttgagacg atcctggccg
      721 gaacaccgct tgaatcctca tctctctccg agaacccgtt cgggttgagg gttttaggcg
      781 tgaaataacc atcgttagaa aaaccggggg cccggggtca ctccccgccc ccctgttccc
      841 gtgcgatccg ttcctcgcct ttcttcgatc gttcgtgctc cgcccgcggc gtcggggtct
      901 ttggcgcccc cggcggccgg ctgggatccc gcccctcgtt gccgagctcg gccgggaccg
      961 ggctgtagac gatatcggtc cccttatcct cgaacggtat ctcctcatcg ggttgctcgt
     1021 cctgtctcct cctccgctct tctgccatac gcatcactcc cgggtgatcc ctgccggaag
     1081 gttccggacc cataaaagtc taccggcgcc ggagccagcc tccggccacc gcgaccgcgc
     1141 cggccccgat gagcgccccc gcccccgcgg cccgggtggg ggttgccgcg ggtgtcgcct
     1201 tttctgccgg cgtcgtggtt atctctaccg gcacatcggc cgtccggaat acgaaaaccc
     1261 gctcccccgc gcctgcggcg atgtaaccgc cgtttgcgga gagggcgacc gacgtcacgg
     1321 gcgcaccggc gtcaaacgac cagagcacgc ccccatcgcc cgagagaagg cgcaaagttc
     1381 cgccggcggt cccggccgcg acgacctctc cctgctccga tacgtctacg gcgaggacgc
     1441 cgctttcggt gtggttctgc cagataaggc cctcccgcgg ccggtagcaa tccaggtcgc
     1501 ccccttgctt cccgatgatg acgaagcgtc cgtcggccgg gagggcgagc gcgaggatgg
     1561 gtgccccggc ctgcacaacc caggggagtc ccccgctcct cccgtagacc tggaggaccc
     1621 ggtcccgtcc tccggcggcg acggtcgttc cgtcgcggga ggcgtcgacc gcggtgaccg
     1681 gaccgtacgc gatcccctgc cagagcaggt tcccccggct gccgatgaga tgaatgacac
     1741 cgttgtcgct tccggcggcg atgtagttgc cgtcggctgt aaaagcaatg gacgatacgg
     1801 ttgccccggc tcctgtcgag aagttccagc cctccttacc ctggtggtca aagagggtga
     1861 cgcggctgct ccccaccgcg agggaccggc cgtccccgga gaaggcaagg tcacgaacgg
     1921 gtcctgcgac cctttctgtc cagtagcggt tgccgctcgc cccgtcgagc aggatgacgt
     1981 cgcccgcgtt ggttccggcg gctacatagc cccttgcacc ggcgacggca accgccgtta
     2041 ccggatcccc ggcgctcgta tcccaggcaa ccgaaccgtc ctgccgcagg caggcgatca
     2101 tccctccgtc ggtccctgcg accgcgtatc ggccgtctgc gctgatcgca acgtcgttga
     2161 cggcgcttcc catcgatacc cccccgccgg caaccatcgg ggccaggacg gcggcaagga
     2221 cgatcatcag tgctgttctt ctcatggtaa cgttctctcc catgccttct ttctcggaga
     2281 gttccttaaa ttgtttcacc atgctccgga gaaggttgaa ggcagccggt gagcaaccaa
     2341 tcccccgggt gagcatcacg ataaaacgag cagaagaagc ggtcgcccgc ttcatgcagg
     2401 gctacaactg tgcgcaatcg gtcagttcgg tcttctccca cgacgccggg gtgcccgagg
     2461 agaccatcct ccgggcggca accgggttcg gcggcgggat ggggcgcacc gggggcgctt
     2521 gcggggcggt gtcgggggca gtcctcgtca tcgggctcat ggtcgggagt gccggaccgg
     2581 atgagaaaga ggcgaaggac ctgacctacg acctcacgcg agagttcgtc gagcagtttg
     2641 ccgaaaggac cggcagtgtc tcctgcacgg aactcctcgg ctgcgacctc tcgaccgatg
     2701 aggggcgagc gcgggcccgt agagataacc tcacccggac cctctgcccc cggtacatcc
     2761 ggaatgcggt cgatatcctc gaagaggtcc tcgcacccgt catttccggg cggcccgccg
     2821 gccggtgaga gggccgggga gggacggcag ccggagcatc cgcgcgatct cgccggtgcc
     2881 gaggttcttt gccgtgagtg cccgtttctc gaccaggttc caggagagga acgcgagagc
     2941 gaacgtcacg gcaaacgaga gcccgaagag cgccgggagg aggagcgcgt ttgccgtgac
     3001 caggatcagc gtctgctgaa ccgggtagtg gtagatgtag atgccgtagg agaagtcgcc
     3061 cgacctcccg aaggtgttca ggtaggggat ggggagatgc gcggcgtaga tggtgaggta
     3121 ggggatgcag accaccccgg cgacggttgg gtaaggcgtc agggtcgaga gaccgagcag
     3181 gaggagcagc gccccggcga cggccggccg gtaggtgatc cgctcccggt gaaggtagag
     3241 gtatgccccg atcaggaagt agagggtgaa ccggaccttc gccatccggg ggtcgtcgaa
     3301 ccagtatatc cagaccagga tgtttacgac ggcaagggcg ggaatggcgc cccatcgccg
     3361 gaggagcccg gcaatcccga ggagggcgac gaccccgtac atggcgacct cgacgggtat
     3421 cgtccagagc gagccgttga cgtaggtcca cgggttc
//
LOCUS       DAOSII010000191         3446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_23907_length_3446_cov_59.843704, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000191 DAOSII010000000
VERSION     DAOSII010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23907_length_3446_cov_59.843704"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PLV90_09990"
     CDS             <1..182
                     /locus_tag="PLV90_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOF97485.1"
                     /translation="REYIVVHEAAHLRERNHSKRFWLLVGDRYPGYRAAESELRRYWV
                     ILERNRIWGSLQDVG"
     gene            complement(441..1994)
                     /locus_tag="PLV90_09995"
     CDS             complement(441..1994)
                     /locus_tag="PLV90_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF97486.1"
                     /translation="MGDQDSRGGEAFIAAGTRDVAAAVLQQVIETSLSGTCIIDREGC
                     IVSASAPLLAIWGYSRENVVGKPVDILWPSRGERPDWIGQALSTGRWAGTVVARRGDG
                     SLFDVRISAGTARDPGTGHPWLILSCLDAAAQQRPEETPGQRRSLECAVAAMPARFID
                     SSQIDPAIDDSLRDLGRACGACRAYLALFNDAKAILGTTHEWCAPGQRPRRGEFQKIH
                     SGNLPRWIGRILNGEMVYMDGTPREGQTQEEERDLLKRHCTTAALMIPLRLHGKVVGF
                     VGLDRCIGDTRLANEDIALIHAAVHIIASALDRKQSEYLFRESENLYQIVLDAITDAV
                     IVVDTGLTLLLANDAFIAWCEELGLATDVVGKDIFTVLPFFPPSTRQQYERVIRTGRP
                     LTSERSFRIDDRAVTLREIRIPIIERGEVARIVTVARDITAQREVEDLRTKAYAQIER
                     NMEQFALLADHIRNPLQAIIGRAELLDDAGATEKIRQQVQRINAIIDQLDERWAESRK
                     LSMFWRKYS"
     gene            complement(2281..2961)
                     /locus_tag="PLV90_10000"
     CDS             complement(2281..2961)
                     /locus_tag="PLV90_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HOF97487.1"
                     /translation="MHQTGTWGDDGVESRANLLTVSVPLLLCGIFFAVVGLFLPAEQW
                     FLLAGMMVAYIVPPLGRETIIPVSILLGIPWWLIAFTLAFLDFAGGLFMAWNFPLVLR
                     VPYVGPRIRRFMTAGRAYLDRRPWLERLYFVGLIAFVSLPFDGSGSIIGSVLGRMLGM
                     TKIEVLSCITIGGIIGSFTIALGIDYIVNLIPAGAGTWVSAAVFLLIGIALLVMYRSY
                     SRGWGADA"
     gene            complement(2985..3428)
                     /locus_tag="PLV90_10005"
     CDS             complement(2985..3428)
                     /locus_tag="PLV90_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthase"
                     /protein_id="HOF97488.1"
                     /translation="MIRVVATGTFDILHPGHLYYLEESRNLGDELSVIVARDANVKHK
                     PRPILPEDQRLQMVRALKPVDRALLGDIHDMFRPIAELKPDIITLGFNQHFDEENLRR
                     RLRERGLDADVVRISGYPGPLASSSKIVEHILKTRGPTPPTGEEE"
BASE COUNT          612 a         1095 c         1110 g          629 t
ORIGIN      
        1 tgcgggagta catcgtcgtc cacgaggccg cccacctccg cgagcggaac cattcgaaac
       61 gtttctggct cctcgtcggc gaccgttacc ccgggtaccg ggcggcggag agcgaactcc
      121 ggcgctactg ggtcatcctc gaacggaacc ggatctgggg ttctctccag gacgtcgggt
      181 gagaggtccc ggcactttgc tacacggttt gagaccgccc tcaggggatc gaaccatcga
      241 ctttccttcg cgcgagcgag cgggtgccga tagcgatacg gtctcttgcg aaacggggag
      301 ggcctggacc ggatgtgagt gatgcctttt cgcggcctcg tatcttcgtg agcgacaggt
      361 ccggatcgtg caggcacact ccggtgctcc cgatccgctg cgcgcagacg agggcggtcc
      421 ggcgctcgaa caggattggc tcaggagtac ttccgccaga acatggagag cttgcgcgac
      481 tccgcccagc gttcgtcgag ttgatcgatg atcgcgttga tccgctgcac ctgctgccgg
      541 atcttctccg tcgccccggc atcgtcgagc agttcggcgc gccctatgat cgcctggagc
      601 gggttcctga tatggtcggc aagcagggca aactgctcca tgttccgctc aatctgagcg
      661 taggctttcg ttcgcaggtc ctcgacctct ctctgggcgg tgatgtcccg ggcgacggtg
      721 acgatccgcg cgacctcgcc gcgctcgatg atcgggatcc gtatctcccg gagggtcacc
      781 gcccgatcgt cgatcctgaa agaccgttcc gacgtcagcg gccgcccggt ccgtatgacc
      841 cgttcgtatt gctgcctcgt ggagggcggg aagaacggca ggacggtaaa gatgtccttc
      901 ccgacgacat ccgtcgcaag tccgagctcc tcgcaccagg cgatgaaggc atcgtttgca
      961 aggaggaggg tgagtccggt atcgacgacg atgacggcgt cggtgatggc gtcgaggacg
     1021 atctggtaga ggttctccga ctccctgaag aggtactcgg actgctttcg gtcgagtgca
     1081 ctcgcgatga tgtggacggc ggcatggatg agggcgatat cctcgttcgc gaggcgggtg
     1141 tcgccaatac accgatctag cccgacaaaa ccaacgactt tcccgtgcaa ccggaggggg
     1201 atcatcagcg ccgcggtggt gcagtgccgc ttcaggaggt cacgctcctc ttcctgcgtc
     1261 tgcccttcgc gaggcgttcc atccatgtac accatctcgc cgttcaggat ccggccgatc
     1321 caccggggga gattgcccga gtggatcttc tgaaactccc cccgccgggg tctctgccct
     1381 ggtgcgcacc actcgtgcgt cgtaccaagg atcgcctttg catcgttgaa gagggcgagg
     1441 taggccctgc atgccccgca cgcacggccg agatcacgga gcgagtcgtc gatggccgga
     1501 tcgatctgcg aggagtctat gaagcgtgca ggcatcgccg cgacggcaca ctccaggctg
     1561 cggcgttggc cgggggtctc ctccggtctt tgctgcgcgg cggcgtcaag gcacgagagg
     1621 atgagccagg ggtgccccgt cccgggatca cgagcggtgc cggccgatat ccggacgtca
     1681 aagagcgacc cgtctccccg ccgggcgaca accgtcccgg cccaccgacc ggtagagagg
     1741 gcctggccga tccagtccgg tctctccccc cgggagggcc agaggatatc tacgggtttt
     1801 ccgaccacgt tctcccggct atagccccag atggcgagca acggagcgct ggcggagacg
     1861 atgcaccctt cccggtcgat tatgcaggtt ccggagagcg atgtctcgat cacctgctgc
     1921 aggactgcag cggcaacatc cctggttccg gcggcgatga aggcctcgcc tcctcgcgag
     1981 tcctgatcgc ccatctatcg ccctcgaggt tcgccggtcg caaaaccctt caccgtatgc
     2041 ggatccatat cccgatcatt ccagacggcc ccgcgtggcc gcaatccccc cgccccgggg
     2101 tgggttgcac gctgccgctc tccgtgtctc cagtccggag cggataggat gatcaatctc
     2161 tcgcactata tactttttct catttgagtt tcggcgatcc acggccatga tcgtgttccc
     2221 ggccgtccgg tgcacgatca ggcgtcggag agaactgccg gctctcgatc ggggagacga
     2281 tcaggcgtct gccccccacc cccgcgagta gctccggtac atcaccagga gcgcgatccc
     2341 gatcaggagg aagacggcgg ccgagaccca cgttccggcc cctgccggga tgaggttcac
     2401 gatatagtcg atcccgaggg cgatggtgaa actgccgatg ataccgccga tggtgatgca
     2461 ggagaggacc tcgatcttcg tcatgccgag catcctcccg aggacggaac cgatgatgct
     2521 ccccgacccg tcgaacggga gactcacgaa ggcgatgagt ccgacaaagt agagccgctc
     2581 aagccacggc cgccggtcca gataggcgcg acccgccgtc atgaaccgtc ggatccgggg
     2641 gccgacgtag ggcacgcgca ggacgagcgg gaagttccag gccatgaaga gaccgccggc
     2701 aaagtccaga aacgccaggg tgaacgcgat cagccaccag gggatgccaa gaaggatgct
     2761 gaccgggatg atcgtctccc ggccgagcgg tgggacgatg taggcgacca tcatcccggc
     2821 gagcaggaac cactgttccg ccgggaggaa gagtccgacg acggcaaaaa agataccgca
     2881 gagcagaagc gggactgaga ctgtgagcag gttcgcccgg ctctcaaccc cgtcgtcgcc
     2941 ccacgtgccg gtctggtgca tgaatcgccg ccccgactcc ccggtcactc ctcttcgccg
     3001 gtgggaggag tgggcccccg ggtcttcagg atatgctcga cgatctttga ggaactcgca
     3061 agcgggccgg ggtatcccga tatcctgacg acgtccgcgt cgagtccccg ttcccggagc
     3121 cttcgccgga ggttctcctc gtcaaagtgc tggttgaacc cgagcgtgat gatgtcgggt
     3181 tttagctctg cgatcggcct gaacatatcg tggatgtcgc cgaggagtgc acgatcgacc
     3241 ggttttagcg cccggaccat ctggagcctc tggtcctcgg ggaggatcgg tcttggttta
     3301 tgcttcacgt tcgcatctcg cgccacgatc acggagagct catcgccgag gttccgggac
     3361 tcctcaaggt agtagaggtg cccggggtgg aggatgtcaa acgtgcccgt tgcgaccacg
     3421 cggatcatcc gaccacctcg aggcgg
//
LOCUS       DAOSII010000192         3407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_24260_length_3407_cov_55.954356, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000192 DAOSII010000000
VERSION     DAOSII010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24260_length_3407_cov_55.954356"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..603
                     /locus_tag="PLV90_10010"
     CDS             178..603
                     /locus_tag="PLV90_10010"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOF97489.1"
                     /translation="MKPQNLSVEEKRRLSGLIVASLSQREEVLFAYVYGSFPRGAFRD
                     IDITVFLADGSNGIADPLRYETALEQELEDIAGVPIDVRVLNAAPLPFAYSVLKSGDL
                     LLSRDERARCDFACRTYTHYHDFAYYRKRYRREALGLLR"
     gene            complement(762..1694)
                     /locus_tag="PLV90_10015"
     CDS             complement(762..1694)
                     /locus_tag="PLV90_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOF97490.1"
                     /translation="MSGTVMDLLVVADQILILVLLIAVGYAAVSTKIMDPRATRGLSA
                     LLVNVTIPALIIASMQVPFTPERLAGAEILLLATGVLFAFSFALAWAVSKAMQVPPAE
                     EGVLQFAIVFGNVGFMGFPVALTLFGEESLFYVAIFNLVFNILVFSVGIAMLTGGRGK
                     EFDPKLLANPGIAAAIVGFALFLGSIEIPGPFIDSIDLLGGVTTPLAMIIVGAMLATF
                     PAREMVGNWRIWVASAILLLAVPVAYCCLFAPVFADPFINGVMITMAAMPAAANTVIF
                     AEQYGANSRLASQIVFVSTIGSLVTIPLVATVLL"
     gene            complement(1852..2214)
                     /locus_tag="PLV90_10020"
     CDS             complement(1852..2214)
                     /locus_tag="PLV90_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97491.1"
                     /translation="MRPKLKRIEGERKTFTGTFARFGKKDRYRPKKVGDEWVTHDLTV
                     LLVDVRDSDGNPVTDHLWFNYTKGFASLGDLQEGDVIRFDARVRPYIKGYVNQREYID
                     EREVDYKLAYPTKVARAR"
     gene            complement(2328..3245)
                     /locus_tag="PLV90_10025"
     CDS             complement(2328..3245)
                     /locus_tag="PLV90_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOF97492.1"
                     /translation="MVTEFLVVADQIFILVLLIAAGYVAVSTKIMDPGATRGLSALLF
                     NITIPALIVSAMQVPFTSERLADTGTMLLATGAFFALSFVVARIASRTMAVAPQKRGV
                     FEFAIVFGSVSFMGFPVARALLGTGSLFYVALFNIVFNILLFSAGIAMLAGEKGFDPR
                     LLANPGIVATIAGFLLFLASVEIPSPFIDAIDLLGGVTTPIAMIIVGAMLATLPAREM
                     IGNWRVWATSAVILIALPVAYCCLLSTIFPDPLINGTMIMMAAMPAAAGTPVFAEQCG
                     ADARLASQIVFVSTIGSLVTIPLVATVLL"
BASE COUNT          694 a         1013 c         1075 g          625 t
ORIGIN      
        1 ctccggccgg gatccgggag cgatttatgc gggcgatccg ggggtgatta cccctccggt
       61 ctccccgatg ggataccccg ggtcgtcatg tcctgatccc gcaccggcca cggcgaggag
      121 aaccgctcca cccaccagaa cgcaaccctc atcatcgccg gcgccaatca tatcgtgatg
      181 aaacctcaga acctctccgt cgaagagaag cggcggctct ccggcctgat cgttgcctcg
      241 ctctcacaga gggaggaggt tctctttgcc tacgtgtatg ggtcgtttcc ccggggtgcc
      301 ttccgtgaca tcgatatcac cgtctttctc gcagacggca gcaacgggat tgccgatccg
      361 ctcaggtatg agactgcgct cgaacaggaa ctggaggata tcgcaggagt acctatcgac
      421 gtcagggtct tgaacgccgc tccccttcca ttcgcgtact cggtcttgaa gtccggagat
      481 ctgctcctct cccgtgatga gcgggcacgc tgcgacttcg cgtgcagaac ctatacccac
      541 tatcacgatt tcgcctacta ccgaaagcgt tacaggaggg aagcacttgg tctcctacga
      601 tgacgaaaaa gtcaccgcac tctcattgga actggtgcag gcgtgcaggc gcttgagaga
      661 tctcgcccgg atgtaaaaag aggtcaccat cggggatccc catctgattg ccagcgcaaa
      721 gtaccatctc atcgtcggta tcgaggcggc tatcgctctc tctacagcag caccgtcgcg
      781 acgagcggga tggtaacgag cgatccgatc gtcgagacga agacgatctg cgacgcgagc
      841 cttgaattcg cgccgtactg ctccgcgaat atgacggtgt ttgcagcggc cggcatcgcg
      901 gccatcgtga tcatgacacc gttgatgaag gggtcggcaa agaccggggc gaagaggcag
      961 cagtaggcca ccgggacggc gaggaggagg atggcgctcg cgacccagat ccgccagttc
     1021 ccgaccatct ctcgggccgg gaacgtcgcg agcatcgccc cgacgatgat catggcaagc
     1081 ggcgtcgtca cgccgccgag gagatcgatc gagtcgatga aggggcccgg tatctcgatc
     1141 gatccgagga agagggcgaa cccgacgatt gcggccgcga taccggggtt tgcgagcagt
     1201 tttggatcaa attccttccc cctccctccg gtcagcatcg cgatcccgac cgagaagaca
     1261 aggatgttga agacgaggtt gaatatcgca acgtaaaaga gcgactcctc cccgaagagt
     1321 gtcagggcga ccgggaaccc catgaacccg acgttgccga agacgatcgc gaactggaga
     1381 accccttcct ctgcgggcgg gacctgcatg gccttcgaga cggcccaggc aagggcgaac
     1441 gagaaggcga agaggacccc ggtcgcgagg aggaggatct ccgcaccggc gaggcgctcg
     1501 ggggtgaaag ggacctgcat cgaggcaatg atcagcgccg gtatggtgac gttgacgagg
     1561 agggcggaga gcccccgggt ggcccggggg tccatgatct ttgtggagac ggcggcgtaa
     1621 ccgacggcga tcagcagcac caggatgagg atctggtcgg cgacgaccag gagatccata
     1681 accgtcccgc tcatgggtgg ggcactccct cttgctccac cagtgtgggt gccgctactc
     1741 ctttacggtt ctgctcgggg tcggaggtat ggcgagcgag cctgtccggg aggcgctcta
     1801 ccggagttat cgcgcgacgt tatcccgcgg ggcgtctcgc ctgccggcta ctcaccgggc
     1861 ccgggcaacc tttgtcgggt aggccagttt gtagtcgacc tcccgctcgt cgatatactc
     1921 cctctggttg acgtagccct tgatgtaagg cctgaccctg gcgtcgaacc tgatcacgtc
     1981 cccttcctgc aggtctccga ggcttgcaaa ccccttcgtg tagttgaacc agagatgatc
     2041 ggtgaccggg ttgccgtcgc tgtcccggac atcgacgagc aacaccgtca ggtcgtgggt
     2101 gacccactca tccccgacct tctttggcct gtaacggtct ttcttcccga accgtgcaaa
     2161 cgttccggtg aatgtcttcc gttccccctc gatccgtttc agcttcggtc tcacgatctc
     2221 actcttcccg gcagaccctt aacgatcagg gactgcatct ttccgccatg ttcaatcgga
     2281 gcgatatacc ataacggtta tgcaagcggg gtgaaagtgt attcccccta cagcagcacc
     2341 gtcgcgacga gcgggatcgt aacgagcgat ccgatcgtcg agacgaagac gatctgtgac
     2401 gcaagccggg cgtcggcgcc gcattgctcc gcaaagacgg gggtgcctgc agcggccggc
     2461 atcgcggcca tcatgatcat ggtgccgttg atgagagggt cgggaaagat tgtggagagg
     2521 aggcagcagt aggcaaccgg tagagcgatg agaatgacgg cacttgtcgc ccagacccgc
     2581 cagttcccga tcatctcccg ggcggggagc gtcgcgagca tcgccccgac gatgatcatg
     2641 gctatgggtg tcgtcacgcc gccgaggagg tcgatcgcgt cgatgaaggg cgaggggatc
     2701 tcgaccgatg cgaggaagag gaggaacccg gcgattgtag ccacgatgcc gggatttgcg
     2761 agcagtcgcg ggtcaaatcc cttctctccg gcgagcatcg caattccggc cgagaagagg
     2821 aggatgttga agacgatgtt gaagagggcg acataaaaga gggaaccagt gcccaggagc
     2881 gcccgggcga ccggaaaccc catgaaactg acactcccga agacgatcgc gaactcaaaa
     2941 actcctctct tctggggcgc taccgccatc gttcgcgatg ctatccgggc gacgacgaac
     3001 gagagggcga agaaggctcc ggttgcgagg agcatcgtcc cggtatccgc gaggcgctcg
     3061 gaggtgaagg gcacctgcat ggcggagacg atcagcgccg gtatggtgat gttgaagagg
     3121 agggcggaga gcccccgggt ggccccgggg tccatgatct ttgtcgagac ggcaacgtag
     3181 ccggcagcga tcagcagcac caggatgaag atctggtcgg cgacgaccag aaattccgtg
     3241 accataccgc tcatgaacgg gctccaccct tccggataca gcagtgtggg ttctgataac
     3301 ccattacggt tctgccgtgg gggagatttt tctctcggca ggactctatc tccctgtatg
     3361 ccggaaaaac cgttctacct cgccgtctcg gcgctcatcc gggacgg
//
LOCUS       DAOSII010000193         3403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_24295_length_3403_cov_36.693548, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000193 DAOSII010000000
VERSION     DAOSII010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24295_length_3403_cov_36.693548"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..273)
                     /locus_tag="PLV90_10030"
     CDS             complement(109..273)
                     /locus_tag="PLV90_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97493.1"
                     /translation="MRVFWPGTELVRQRLQAAVERFVEDENILRATLSSSFAGGRAVS
                     GSDLEVLARD"
     gene            complement(601..1101)
                     /locus_tag="PLV90_10035"
     CDS             complement(601..1101)
                     /locus_tag="PLV90_10035"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOF97494.1"
                     /translation="MNAKTFAALAGAMRKDAAVGQKTVSGLLNWNGGAHSTMIVRNFV
                     IPADEPAALGGTDRGASPMELVLAGLMSCVAVSIAYSAAEEGIDLHAIAIDVEGDIDL
                     RGLFGVADDVRPGFEEIRLIVRVDADASEETIEDLVHRGYRRSPAVDSLTGRVPVRVC
                     IGREGV"
     gene            complement(1098..3170)
                     /locus_tag="PLV90_10040"
     CDS             complement(1098..3170)
                     /locus_tag="PLV90_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOF97495.1"
                     /translation="MTPEKPVQGRSPPNRLIHEQSPYLLQHADNPVDWHPWGEEAFAK
                     ARKEDKPVFLSIGYSACHWCHVMEKESFADPVVAKLLNDVFVCIKVDREERPDIDQIY
                     MAAAHVLAGSGGWPLTILMTPDKKPFFAASYIPKESRYGMTGLLDLIPRISKVWRDRR
                     GELEEAGDCVFGALQSAPSPEGDIPDAAIDEAYETFLRIFDEENGGFGDAPRFPAPHN
                     LIFLLRYAHGTGKEPAYAMAEKTLHAMRQGGIFDQIGWGFHRYATDVAWAVPHFEKML
                     YDQALLAMAYTEAYLATGREEFARTARETIAYVLRDMTGPGGGFYSAEDADSEGEEGK
                     FYTWTKAEILDVLGEDGERFSRIFGIADRPGRQVLRRRRPLASWAHEFSMPEEDLAWF
                     VEGARQRLFQAREQRVRPAKDDKILTDTNALMIAALAGAARAFDDPGYLAAAEKALAF
                     VLENLRDEDGRLLHCRGVAATLDDYAFMLRALIEVYEASFSPAYIRTAVDLARDMIAR
                     YLDRGGFFFTPDDTDLPVRQKTVYDGAVPSGNGVAMHALFVLGRMTANLELEAVADGV
                     LRAFAGAVRKAPAAAPGFLTGLLFMRGPGFEVIIAGVPGAEDTRALVGAVRSRYTPEA
                     VVIFRPSDEEEPDITRVAGFTRDVVAIGGKATAYVCINYACDIPTTEPAEMLRLLASR
                     ERPPEPVI"
     gene            complement(3237..>3403)
                     /locus_tag="PLV90_10045"
     CDS             complement(3237..>3403)
                     /locus_tag="PLV90_10045"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HOF97496.1"
                     /translation="DASRGDVRYVGTYAVGCPVDTNRAKATFRNGLLTVDVPYVQPAA
                     EETKEIPIAE"
BASE COUNT          583 a         1159 c         1087 g          574 t
ORIGIN      
        1 cccggctggc aagggagttg tcaacctcgt ctaccgtgga gggaaagaga tcgatcccga
       61 tgccggagca gtacgggagg taggcgggaa tccgctcgag gggggcgatc aatcgcgcgc
      121 aagaacctcg agatcactcc cggaaacagc cctgccgcca gcaaacgaac tggagagggt
      181 ggcgcggagg atgttttcgt cctccacgaa cctctccacc gccgcctgca gtcgttgccg
      241 gacgagttcc gtaccaggcc agaagactct cacagagttg aaaaactctt tccgcgccac
      301 tgattgcatc atcccggcga agtagtcggc cggcttcctc cggggaaaac ccttccggga
      361 tatacgccgg cttcctccgg ggtatacagg cggtcgaggc tgcgtgcgac cgaacagagg
      421 ccggctgccc ggagggcaaa gcggccccat gtctctggta gacggccttt atcgcctttt
      481 cggcggctgc tgcgatacga aacatccgct caaagaaccc ggtatcccgg gcctgcccgg
      541 cgggcgagaa gattcgccga accggagccg tccatctccc cggtgccgaa gacaccgcta
      601 tcagacaccc tcccgcccga tgcagaccct gaccggcacc cggcccgtga gcgaatccac
      661 ggccggcgag cgccggtagc cgcggtgcac gaggtcctcg atcgtctctt ctgacgcatc
      721 ggcatccacc ctgacgataa ggcggatctc ctcaaacccc ggccgcacgt cgtcggcaac
      781 cccaaagagc cctctcaggt cgatatcgcc ctcgacgtcg atcgcaatcg cgtgaaggtc
      841 gattccctcc tccgccgcgc tgtaagcgat cgatacggcg acgcaggaca tgagccccgc
      901 gagcaccagc tccatcgggc ttgccccccg gtcggtcccg ccgagggcgg ccggctcgtc
      961 ggccgggatg acgaagtttc gtacgatcat cgtcgagtgc gcaccgccgt tccagttcag
     1021 gaggccggag acggtcttct gccccacggc cgcatccttt cgcatcgccc ccgcaagggc
     1081 ggcaaacgtt ttcgcgttca tatcaccggc tccggcggcc tctcccgcga cgcaaggagc
     1141 cggagcatct cggccggctc ggtcgtcggt atgtcgcagg cgtagttgat gcagacgtac
     1201 gccgtcgcct tccccccgat agccacgacg tcccgggtga acccggcaac ccgcgtgata
     1261 tcaggctctt cctcatcgga gggccggaat atgacgacgg cctcgggcgt gtagcgcgat
     1321 cggacggcgc cgaccagggc gcgcgtatcc tccgcgcccg ggacgccggc gatgatcacc
     1381 tcaaaacccg gcccccgcat gaagaggagg ccggtgagga aaccgggggc ggcggcaggc
     1441 gccttccgga cagcgccggc aaaagcgcgc aaaaccccat ccgccaccgc ctcaagctcg
     1501 aggttcgccg tcatccggcc gaggacgaag agcgcatgca tggccacgcc gttcccggag
     1561 gggaccgccc cgtcgtacac cgtcttctgc cggacgggca gatcggtgtc gtccggcgtg
     1621 aagaagaacc cgccgcggtc caggtagcgg gcgatcatgt cgcgcgcgag atcgaccgcg
     1681 gtcctgatat acgccgggga gaacgatgcc tcgtagacct ctatgagggc gcggagcatg
     1741 aaggcgtagt cgtcgagtgt cgccgcaacg ccccggcagt ggaggagccg cccgtcctcg
     1801 tcgcgcaggt tctcaagtac gaacgcaagc gccttctccg ccgccgccag gtagcccggg
     1861 tcgtcgaagg cccgggcggc cccggcaagg gcggcgatca tgagggcgtt cgtatcggtg
     1921 aggatcttgt cgtcctttgc cgggcggacc cgctgctccc gggcctggaa gagtctctgc
     1981 cgggcaccct cgacgaacca cgcaaggtcc tcctccggca tcgagaactc gtgagcccat
     2041 gacgcgaggg gccggcggcg gcggaggacc tgcctgcccg gtctatccgc gatgccgaat
     2101 atacgggaga accgctcgcc gtcctccccg aggacgtcga gtatctcggc cttcgtccag
     2161 gtatagaact tcccctcctc cccctcgctg tccgcatcct ccgccgagta gaacccgccg
     2221 ccgggaccgg tcatatcccg caggacgtag gcgatcgtct cgcgggcggt ccgggcaaac
     2281 tcctccctcc ccgtcgcaag gtaggcctcc gtatacgcca tggcgaggag cgcctggtcg
     2341 tagagcatct tctcgaagtg cgggacggcc cacgccacgt ccgttgcgta gcggtgaaac
     2401 ccccacccga tctggtcgaa tatgccgccc tgccgcatgg cgtgcagggt cttttcggcc
     2461 atcgcatacg ccggctcctt cccggtcccg tgcgcgtagc ggagcagaaa gatcaggttg
     2521 tgcggggccg gaaaccgggg cgcgtcccca aacccaccgt tctcctcatc gaatatgcgg
     2581 aggaacgtct cgtaggcctc gtcgatcgct gcgtcgggta tatcgccctc cggggagggc
     2641 gcgctctgca gggcgccaaa cacgcagtcg ccggcttcct cgagttcccc tcgccggtcc
     2701 cgccagacct tgcttatccg ggggatgagg tcgaggaggc cggtcatacc gtagcggctc
     2761 tccttcggta tgtagctcgc cgcaaagaac ggttttttgt ccggtgtcat gaggatggtg
     2821 agcggccatc ccccggaccc ggccagcacg tgggcggccg ccatgtagat ctggtcgata
     2881 tccggccgct cctcgcggtc gaccttgatg cagacgaaga cgtcgttcaa gagctttgca
     2941 accaccgggt cggcaaacga ctccttctcc atgacgtggc accagtggca ggccgagtag
     3001 ccgatggaga ggaataccgg tttgtcctct ttgcgcgcct tcgcaaatgc ctcctccccc
     3061 caggggtgcc agtcgacggg gttatcggcg tgctggagca ggtaggggct ctgttcgtgt
     3121 atcagccggt tcggtggcga tctcccctgc accggcttct ccggggtcat actcctgacg
     3181 tgccggtaca ggctgataaa ggtgacgccg gcggatagaa aagagagggt ttcccctcac
     3241 tccgcgatgg ggatctcttt tgtctcctcc gcggccggct gtacgtaggg aacgtccacg
     3301 gtcaggaggc cgtttcggaa cgtcgctttc gcccggttgg tatcgaccgg gcagccgacc
     3361 gcgtaggtgc cgacgtagcg gacgtccccg cgggacgcat cga
//
LOCUS       DAOSII010000194         3378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_24530_length_3378_cov_47.101113, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000194 DAOSII010000000
VERSION     DAOSII010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24530_length_3378_cov_47.101113"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..1276
                     /locus_tag="PLV90_10050"
     CDS             107..1276
                     /locus_tag="PLV90_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF97497.1"
                     /translation="MTFDTKPVWGLSAAHLVTDLYSPVLPAILPLLIAEQGYSFFLAG
                     LIVTVYNLTSSLIQPLIGWAFDTRGFAVHVGTSVAIGAIFISIVGLLNNYTLVLASVA
                     VAALGHAFFHPSALGTVSRLTRDASRGRITSYFVIGGNLGYAIGPICAGVAVGLMGLP
                     GLVILAVPGLVMAAVLRQVLPAPDRLKAAPAADPGKRPAYRAIALLVTASGLRAWAIF
                     GSVAYLPTVLTLRGIDLVTANLLVSGMLIAGVVGQLVGGALSDRYGRKEYTILGLVTA
                     IPPFVVFLSTGGILSLVALMIFGFILWSTFAVTVAMGHEIAPGNVGLISGLMLGLAVG
                     VGGMGVAVTGWIADMTSLSLGLMTIPLAIGLALPLFLAVPYPWKSLSQRRSPVPG"
     gene            1323..2972
                     /locus_tag="PLV90_10055"
     CDS             1323..2972
                     /locus_tag="PLV90_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HOF97498.1"
                     /translation="MVTRLRRYRQIADVLAKYGFGILVEEVFPGIGRLWKAPVPADEK
                     QSVYERIRLALEELGPTYIKFGQIMSTRRELLPPELIAELQRLQDRVDPLPFEEIRPV
                     IRKYCPNLEECFDIIEADPVAAASLSQVHRAVMKDGQVVALKVQRPGIVDLIEADILI
                     LQSLARRVESLFPDLRVYNLPGMVDEFAVQIRRELDFSQDGMNAERLRRNMRDIPCVK
                     IPRIHWGITGPFLLAMDYVEGVRIDDVAAIRAFGLFPEEIADIGFQAYVQQIFVDGFF
                     HGDPHPGNLFVTEQGEVVFLDYGIVGVLRPERKRMFIDLLLAMTRTDVAGVVSALERL
                     DVRIRPSDLDAVKDDLYLVLLDYREMSLERMNFATALGGLTDTLRRYHIRVPPTLMLM
                     MKVIIMVMDIGTRLDPAFNFDRRIHPYLTDIVMQERFSPETMANAVRSLASAAEGLLA
                     IPENVNDTLKTLSEGTVTIDLEDTDLKRITDVIDQTSDKVIVGLVVAAVVVGSSLVLR
                     VTELPVPGYVTTLAVLGYVFAVLFGFYAVYNALRHGHVLRR"
     gene            complement(3043..3324)
                     /locus_tag="PLV90_10060"
     CDS             complement(3043..3324)
                     /locus_tag="PLV90_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HOF97499.1"
                     /translation="MTEEDIEATRAEAKAYAKEKGYVLNVDEKQLSAVLRGLARNKER
                     LGAAYCPCRLRSGDPEKDRIIVCPCLYHEQEIEEQGSCHCRLFFKKKAE"
BASE COUNT          518 a         1151 c         1025 g          684 t
ORIGIN      
        1 gaggtgatcc ggtgcgcgtt tcccctccgg cggacgtttg cccaccaccg caaagagcga
       61 gtggatatta ccgtagaggt catacattta cgacggatct aataccgtga ctttcgatac
      121 caaacccgtc tgggggctct ccgccgccca cctggtgacc gatctctact cgccggttct
      181 ccctgcaatc ctgccgctcc tcatcgcgga gcagggctac tcgttcttcc tcgcggggtt
      241 gatcgtcacc gtctacaacc tcacgtcgtc cctcatacaa ccgctcatcg gctgggcctt
      301 cgatacccgg gggtttgccg tccacgtcgg gacgagcgtt gccataggcg cgatcttcat
      361 atccatcgtc gggttgctca acaactacac gctcgtcctt gcatccgtcg ccgtcgcggc
      421 gctggggcac gccttcttcc acccgagcgc gctcgggacc gtcagccgcc tgacccggga
      481 cgcgagccgg ggtcggatca cctcctactt cgtcatcggc ggaaacctcg gctacgcgat
      541 cggccccata tgcgccgggg tggcggtcgg cctcatgggt cttcccggcc tcgtcatact
      601 cgccgtcccc ggccttgtga tggcggcggt cctccggcag gttctccccg cgccggatcg
      661 cctgaaagcc gcgccggcag ccgacccggg caagcggccc gcttaccggg ctatcgccct
      721 cctggtcacg gcttcgggcc tccgggcatg ggcgatattc ggttcggtcg cctacctccc
      781 gaccgtcctc accctgcggg ggatcgacct cgtgaccgca aacctgctcg tctcggggat
      841 gctcatcgcc ggggtcgtgg ggcagctcgt cggcggcgcg ctctcggatc gctacggccg
      901 caaggagtac acgatcctcg ggctggttac cgccatcccg ccgttcgtcg tcttcctctc
      961 gaccggcggc atcctctcgc tcgtcgccct gatgatcttc gggttcatcc tctggtcgac
     1021 cttcgccgtc accgtcgcca tgggtcacga gatcgccccg gggaacgtcg ggctcatctc
     1081 cggcctgatg ctcgggctcg ccgtcggcgt gggcggcatg ggtgtcgcgg tcaccgggtg
     1141 gatcgccgat atgacgtcgc tctcgctcgg cctcatgacg atcccgctcg cgatcggcct
     1201 tgccctcccc ctcttcctcg ccgtccccta tccctggaag tcgctctcgc agaggcggtc
     1261 ccccgtcccg ggataacccc accccaccct cttaagtacc gggagtgcag agtccatccc
     1321 cgatggtcac ccggctccga cgctaccggc agattgcgga tgtgctggcg aagtacggtt
     1381 tcggtatcct ggtcgaggag gtcttccccg gaatcgggcg gctgtggaaa gcccccgtgc
     1441 ccgctgatga gaaacaatcg gtgtacgagc gtatccgcct tgcgctcgag gagctggggc
     1501 cgacctacat caagttcggc cagatcatga gtacacggcg ggaacttctc ccccccgagc
     1561 tgatagcgga gctgcagcgg ttgcaggacc gggtcgaccc gctcccgttt gaggagatcc
     1621 ggcccgtgat ccggaagtac tgtccgaacc tcgaggaatg tttcgatatc atcgaggccg
     1681 atcccgtcgc ggcggcctcc ctctcgcagg tgcaccgggc cgtgatgaag gatggccagg
     1741 tcgtcgccct caaggtgcag cgccccggta tcgtcgacct gatcgaggcc gatatcctca
     1801 tcctgcagtc gcttgcccgg cgggtggagt cgctcttccc ggacctgcgg gtctacaacc
     1861 tgccgggtat ggtggatgag ttcgcggtcc agatccggcg cgagctggac ttctcccagg
     1921 acgggatgaa cgcggagcgt ctcaggagga acatgcgcga catcccgtgc gtgaagatcc
     1981 cgcggatcca ctgggggatc accggcccct tcctgctcgc catggactac gtcgaggggg
     2041 tccggatcga cgacgtggcg gccatccgcg cgttcggcct cttccccgag gagatcgccg
     2101 atatagggtt tcaggcctac gtccagcaga tattcgtcga cgggttcttc cacggggacc
     2161 cgcaccccgg aaacctcttc gtgacggagc agggcgaggt cgtcttcctc gactacggca
     2221 tcgtcggcgt cctccgcccg gagaggaagc gtatgttcat cgatctcctc ctcgccatga
     2281 cccggacgga tgtggcgggc gtggtctcgg cgcttgagag gcttgacgtg cgcatcaggc
     2341 cctccgacct tgatgccgtc aaagatgacc tttacctggt cttgctcgac taccgggaga
     2401 tgagccttga gcggatgaac ttcgcgacgg cgctcggggg cctgaccgac accctccggc
     2461 gctaccacat ccgggttccg cccaccctga tgctgatgat gaaggtgatc atcatggtga
     2521 tggatatcgg cacccggctc gacccggcgt ttaacttcga ccggcggatc catccctacc
     2581 tgacggatat cgtgatgcag gagcggttct cccccgagac catggcaaat gcggtgcggt
     2641 cgcttgcaag cgctgcggag gggctgcttg ccataccgga gaacgtgaac gataccttga
     2701 aaaccctctc ggaggggacc gtcacgatcg atctcgagga taccgatctt aaaaggatca
     2761 ccgatgtcat cgaccagacg agcgataagg tcatcgtcgg gctggtggtc gccgcggtcg
     2821 tcgtggggtc ctcactggtg ctccgggtga ccgagctccc ggtccccggc tacgtcacga
     2881 cgcttgccgt cctgggctac gtcttcgcgg tgctgttcgg gttctacgcg gtctacaacg
     2941 ccctgaggca cggccacgtc ctccggcgct gaccggtccc ccctcccgct caacgcggag
     3001 agcctgccgg cccaaaaaaa gatcatccgg gccgggggcg gttcactcgg ctttcttctt
     3061 gaagaagagc ctgcagtggc aggacccttg ctcctcgatc tcctgctcgt ggtagaggca
     3121 gggacagacg atgatccggt ctttctcggg atcgccgctc cggagcctgc agggacagta
     3181 tgccgccccc agccgctcct tgttccgggc aagcccgcga agaacggccg agagctgctt
     3241 ttcatcgacg ttcaagacgt aacctttctc ctttgcgtag gctttcgcct ccgcgcgcgt
     3301 cgcctcgata tcctcttcgg tcatactctt aatcaggcct cctgccgtcc gtactcttcg
     3361 atatgggttg tcatcaag
//
LOCUS       DAOSII010000195         3327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_25005_length_3327_cov_44.865831, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000195 DAOSII010000000
VERSION     DAOSII010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25005_length_3327_cov_44.865831"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="PLV90_10065"
     CDS             complement(<1..1024)
                     /locus_tag="PLV90_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97500.1"
                     /translation="MSCSAVPIQAGETAAVTESAPITATSGGPGILPPESPVKVVFIH
                     HSTGENWLADDDGGLGIALRENNYFVSDTNYGWGPDAIGSSTDIGHWWTWFRSTQSPG
                     YLAALFNESGEHSSGRYSRLAADPGGQNRIVIFKSCFPNSALQGSPSDPVPSIGDNPL
                     KGNDCGSEHHTVANAKGIYIDILEYFGTKPETLFVVIAAPPLSDGTYAANARAFNQWL
                     ATEWLSGYPGENVFVFDFYNVLTTNGGNPEVNDLNATAGNHHRWWNGAVQHQVDLSFN
                     TSAYPSPEGDDHPAQAGNRKATAEFVPLLNYAYNRWANVTGDPTDLRADFTANVTAGP
                     APLAVQF"
     gene            1749..1913
                     /locus_tag="PLV90_10070"
     CDS             1749..1913
                     /locus_tag="PLV90_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97501.1"
                     /translation="MCGRRYKQRKSVLIVTGDLPLAGGLDSEGSGEPFHRVARHRAVP
                     TARKGDAGEA"
     gene            1969..>3327
                     /locus_tag="PLV90_10075"
     CDS             1969..>3327
                     /locus_tag="PLV90_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HOF97502.1"
                     /translation="MSGDLVVQLREMMELPAETECIEFKEAKGDYGFDRIGKYFSALS
                     NEANLHGKPAGWLIFGVTDSLPRKIVGSNYRLTPPGLDHLKEEIAQHTNHRITFIAIH
                     ALTVDEKRVVMFEIPPAPRGVPTTWKGVAYGRNHESLEPLALYEIEVIRRQAPVDDWS
                     AMICEGATLADLDPEAIAFAREKYKEKFRERHPDRAKEVDEWDDLTFLAKAKACHQGR
                     ITNTAVLLLGKSEAASLLSPAIAHITWVLRDKGEIEVDYAHFGPPLILAVDRVFSKIR
                     NLTVRYMSGETLFPLEVTQYDPWVIREALHNCIAHQDYRQSARITVTERPDSLMFTNR
                     GDFIPGTVQSVIERDMPPDWYRNPFLAGAMVELKMIDTIGSGIKRMFRVQRDRNFPMP
                     DYDLNKEGVVRVTIPGRVIDERYTRMLIERKDLTLMDVIALDKVQKGYPVTPEERTVL
                     RKK"
BASE COUNT          674 a          939 c         1015 g          699 t
ORIGIN      
        1 tgaactgcac cgcaagcggg gcagggccgg cggtcacgtt tgcggtgaaa tcggcacgaa
       61 ggtcggtcgg gtctccggtg acgttcgccc accggttata ggcataattc aagagcggga
      121 cgaactcggc ggtggccttc cggttgcctg cctgcgccgg gtggtcgtcc ccctccggcg
      181 aggggtaggc ggacgtgttg aaggagaggt cgacctggtg ctgaaccgcc ccgttccacc
      241 agcggtgatg gttgccggcg gtcgcgttga ggtcgttgac ttccgggttt ccgccgttcg
      301 tcgtgaggac gttgtagaaa tcgaagacga agacgttctc tccggggtat cccgagagcc
      361 attcggtcgc gagccactgg ttgaacgccc gggcgttcgc ggcataggtt ccgtccgaga
      421 gcgggggggc ggcgatcacc acgaagaggg tctccggctt cgttccgaag tactcgagga
      481 tgtcgatgta gatccccttc gcgtttgcca cggtatggtg ctccgagccg caatcgttgc
      541 ctttcagggg gttgtcgccg atcgaaggca ccgggtcgga ggggctgccc tgaagggccg
      601 agttcgggaa gcaggatttg aagatcacga tccggttctg tccgccgggg tcggcggcga
      661 ggcgggagta cctgccggag gagtgctcgc cggattcgtt gaagagggcg gcgaggtacc
      721 cgggcgattg cgtgctccgg aaccacgtcc accagtgacc gatgtcggtg gacgatccta
      781 tagcgtcggg cccccagccg tagttggtgt cgctgacgaa gtagttgttc tcccgcaggg
      841 cgatgcccag cccgccgtca tcgtcggcaa gccagttctc gccggtggaa tggtggatga
      901 agacaacttt gaccggggat tccggcggca ggattcccgg tccacctgac gtggccgtga
      961 tgggagccga ctccgtgacg gcggccgtct cgccggcctg gatcgggact gcggaacatg
     1021 acatgatcaa caactgtgca aacaccagga tgaacgtgaa tcgaatcaaa gcctttggca
     1081 caaccatact gtaaccctct cgcatctttg tcggaagtgt gaaccgaacg ccttgactcc
     1141 gatccgcccc gtctctccgg acatgacata gcattcgccg tgtcccggta gagcccgtag
     1201 gacgggtcga ttggtcccag acagttgtta cctaccggtt ttgtatcgca atcaatccgc
     1261 tccggatcag tcgcccgctt ccgggaacag cgcggtcgga gcctacggcg aaaaaaggga
     1321 tccgctgttc tcaggtgctc gcactcctga gtccagatgg ctggcaggat aactgtctgt
     1381 ggttaacctc tcccgatcaa tactaactcc tccccaatcc gggtgtataa atatttcgaa
     1441 atcgtcaggc aatccttcga aaaagtacgc ctttttgaaa cgttttttct tctccttccc
     1501 ccatcacctt cggcttcgcc ctatctttgt ggcaacagac ctcatttttg cccgaggcgt
     1561 atcgggttcc cttattatgg acgaaattct tcccgttttc cggtgcgcgc tgttttagcc
     1621 ggtaaaataa ggtaatgcgg cctccatcgc tcctccccgc tcccccccgc agaccacctg
     1681 cccgaagctg acgcgcccgt ccccgagggg gtactcccgg actacctcaa gcccggcagc
     1741 gcggcaccat gtgtggccgc cgctacaagc agcgcaagag cgtcctgatc gtcaccgggg
     1801 atctcccgct cgccggcggc ctggatagcg aggggagcgg cgagccgttt caccgggtcg
     1861 cccggcaccg ggcggtcccg acggcaagga agggagacgc aggagaggct taatggagga
     1921 gctggtccaa tcattcatgt gggataggct gttcgggagg agagggcgat gtctggggat
     1981 ctggttgttc aactgcggga gatgatggag cttcctgccg aaaccgaatg catcgagttc
     2041 aaggaagcaa aaggcgatta cggtttcgat agaatcggca aatatttctc agcattgagt
     2101 aatgaagcca acctgcatgg aaaaccggcg ggctggctga tcttcggtgt caccgacagc
     2161 ctgcccagaa agattgtcgg ctcaaattac cgtcttaccc cacctggtct cgatcatctc
     2221 aaagaagaga ttgcgcagca tacgaaccat cgtataacgt ttatcgccat ccacgcactt
     2281 acggtcgatg agaaacgggt ggtgatgttt gagattcccc ccgctccccg cggcgttccg
     2341 accacatgga agggtgttgc atacggtcgg aaccatgaat ctctcgaacc tcttgccctc
     2401 tatgagattg aggtgatacg caggcaggct cctgtggatg actggtcggc gatgatctgt
     2461 gagggtgcca ctcttgcgga tctcgatccc gaggcgatcg cctttgcacg agagaagtac
     2521 aaagagaaat tcagggagag acacccggat cgtgcaaaag aggtcgacga atgggatgac
     2581 ctgactttcc ttgcgaaggc gaaggcatgc caccagggtc ggattacgaa tactgcggtc
     2641 cttctcctcg gaaagagcga ggcggcgtcc ctcctctcac ctgcaatcgc gcatatcacc
     2701 tgggttctca gggataaggg cgagatcgag gtcgactatg cccatttcgg ccccccgctg
     2761 atccttgccg tcgatagagt ttttagcaag atccgaaacc tcacagtccg ttatatgtca
     2821 ggagagacgc tgttccccct tgaggtgacg cagtacgacc cctgggtgat ccgtgaagca
     2881 ttgcacaact gtatcgccca ccaggactac cggcaatctg ctcgcattac cgtgactgag
     2941 aggccggatt ctctgatgtt taccaatcgc ggggatttca ttccgggaac tgtccagagc
     3001 gtgatcgaac gtgacatgcc gccagactgg tatcgaaacc ccttccttgc cggcgcaatg
     3061 gtcgaattga agatgatcga caccatcggc agcgggatca agcggatgtt tcgagtgcag
     3121 cgggaccgca acttcccgat gccggattac gatctcaaca aggaaggtgt ggtgcgggtc
     3181 acgatccccg gccgggtgat cgatgaacgg tatacccgga tgctgatcga gcggaaggat
     3241 cttacgttga tggatgtcat cgctctcgat aaggtacaga agggttatcc cgtcacaccg
     3301 gaggagcgaa ctgttctccg gaagaag
//
LOCUS       DAOSII010000196         3326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_25016_length_3326_cov_53.298991, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000196 DAOSII010000000
VERSION     DAOSII010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25016_length_3326_cov_53.298991"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1715)
                     /locus_tag="PLV90_10080"
     CDS             complement(<1..1715)
                     /locus_tag="PLV90_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-CASP ribonuclease aCPSF1"
                     /protein_id="HOF97503.1"
                     /translation="MVIEEKLRELKEQINKIVPGGITISDVEFEGPELVIYTDDPKQF
                     ADQADLIKVLARDLRKRIVVRPNILEDPERAAQEIRSVVPENAGISDLFFDPETGEVL
                     IEAEKPGVVIGKNGITLREITKQIGWTPKVVRTPPIESTTVKQIRTFLRSVKDERKAF
                     LRKIGHRIHREVTSKDQWLRVTTLGCCREVGRAAFLISTPESKILVDCGEKPGSATNG
                     APYLYVPEIYPLDSLDAVVLTHAHLDHCALVPLLFKYGYEGPVYSTPPTRDLSTMLLL
                     DYLDVVRKETDKIPYTSNEVRTYMKHSIALNYGNVTDIAPDVKLTFHNAGHILGSAIA
                     HFHIGEGLYNIAFTGDFNYQKTRLFSPAVSSFPRLEALFMESTYGGANDIQPQRKDAE
                     EKLYETVSTTIKRGGKVIIPAFAVGRSQEVMLALEEGIRKQKIPPVKIYLDGMIKEAT
                     AIHTTYPEYLNSDLRNQIFREGLNPFLADAFVQVDSPDLREKVIGGDPCVIITTSGML
                     NGGPVMEYLRTLGPDERNSLIFVGYQAEGTLGRRIQKGWREIPLGWRETIVINLEIAT
                     SDGFSG"
     gene            complement(1736..2380)
                     /gene="psmB"
                     /locus_tag="PLV90_10085"
     CDS             complement(1736..2380)
                     /gene="psmB"
                     /locus_tag="PLV90_10085"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit beta"
                     /protein_id="HOF97504.1"
                     /translation="MLDTSQEIMKGTTTVGLIFNEGVVLATEMRATMGNMIASKRAKK
                     IYQITPRIGMTTAGGVGDAQQLVRILQVECNLFEMRRGKTMSVGAASTLLSNYLNQNR
                     YYPYYVQLLMGGFDDEGPSVYSVDALGGATKEEEIVATGSGSPFAYGVLEDQYRPDMK
                     EDEAKDLAVRAVKSAMRRDSASGEDIMVVVITKDKYEEHIERGLQRPSVARSIT"
     gene            complement(2526..>3326)
                     /locus_tag="PLV90_10090"
     CDS             complement(2526..>3326)
                     /locus_tag="PLV90_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOF97505.1"
                     /translation="DDVLRILKRTGISGVPVLKGGELVGIITRKDLLRKAEETQLGLL
                     MTPDPVVIRPDAPLPEAAQLMVRHNIRRLPVVENGSMVGIISVADLIGAIAQMRLAVP
                     IRDNYISKTYALWEETPLSLVGRVLEISGYDAIPILMEDGTLTGIISERDLIRHTRIE
                     DGVEVSDFSNGTDDDEWTWESIRDMHTISYGISRIQLLPIPVKNAMVRNVLAVPINAE
                     VGECALKMRRARVDQLPVVNGNKRLIAMLFDRELIKALLPDQPGLRKN"
BASE COUNT          591 a         1005 c         1008 g          722 t
ORIGIN      
        1 ccggagaacc cgtcgctcgt cgcgatctcg aggttgatca cgatcgtctc gcgccacccg
       61 agcgggatct cccgccaccc tttctggata cgccgcccga gcgtcccttc cgcctggtac
      121 ccgacgaaga tgagcgagtt gcgttcatcg gggccgaggg tcctgaggta ctccatcacc
      181 ggcccgccgt tcaacatacc gctcgtcgtg atgatgacgc aggggtcgcc cccgatcacc
      241 ttctcgcgga ggtccggcga gtctacctgg acaaaggcat cggcaaggaa ggggttcaac
      301 ccctcgcgga agatctggtt tcggagatcg ctgttgaggt actccgggta ggtcgtgtgg
      361 atcgccgtcg cctccttgat catgccgtcg agatagatct tcaccggcgg tatcttctgc
      421 ttccggatcc cctcctcgag ggcgagcatg acctcctgcg accgcccgac ggcgaacgcc
      481 ggtatgatca ccttgccgcc gcgtttgatc gtcgtcgaga ccgtctcgta gagtttctct
      541 tctgcgtcct ttctctgcgg ctggatatcg tttgctccac cgtaggtgct ctccatgaag
      601 agcgcctcga ggcgggggaa cgacgacact gcgggagaaa agagcctcgt cttctggtag
      661 ttgaagtctc cggtgaatgc gatgttgtag agaccctctc cgatatggaa gtgtgcgatg
      721 gccgacccga ggatatgccc cgcgttatgg aaggtgagct tgacatcggg ggcgatgtcg
      781 gtcacgttgc cgtagttgag ggcgatggag tgcttcatgt aggtccggac ctcgtttgag
      841 gtatagggga tcttgtccgt ctccttccgg acgacgtcca ggtagtcgag caggagcatc
      901 gtcgagaggt ccctcgtcgg cggggtcgag tagaccggcc cctcgtagcc gtacttgaag
      961 aggagaggga cgagagcaca gtggtcgagg tgcgcgtggg tgagcactac cgcgtcgagc
     1021 gagtcaagcg gatagatctc gggcacgtaa agatacggcg ccccgttcgt ggcactgccg
     1081 ggcttctcgc cgcaatccac caggatcttg ctctccggcg tcgatatcag aaacgcggcc
     1141 cggccgacct cgcggcagca cccgagcgtc gtgacccgca gccactggtc cttgctcgtg
     1201 acctcgcgat ggatgcgatg gccgatctta cgcaggaacg ccttacgctc atccttcacc
     1261 gaccggagga acgtgcggat ctgcttcacc gtcgtgctct cgatgggcgg tgtccggaca
     1321 accttcggtg tccagccgat ctgttttgtg atctcgcgaa gcgtgatgcc gttcttgccg
     1381 atgaccacgc cgggcttctc tgcctcgatc aggacctcgc ccgtctccgg gtcaaagaag
     1441 agatcggata tcccggcgtt ctccggcaca acggagcgga tctcctgggc cgcccgttcc
     1501 ggatcttcga ggatgttcgg gcgcacgacg atgcgcttgc gcagatcgcg cgcaaggacc
     1561 ttgatcaggt ccgcctggtc ggcaaactgt tttgggtcat cagtgtagat gacgagctca
     1621 ggcccttcga actcgacgtc tgagatcgta atcccgccgg gaacgatctt attgatctgc
     1681 tccttcagct cccgaagttt ctcttcaatc accattcaca tcgtcctaaa aaatatcagg
     1741 ttatcgagcg ggccacggat ggtcgctgca acccgcgctc gatatgttct tcgtatttat
     1801 ctttcgttat cacgaccacc atgatatcct caccggatgc ggagtcgcgg cgcatcgccg
     1861 acttgacggc gcggacggcg aggtccttcg cctcgtcttc cttcatgtcc ggacggtact
     1921 ggtcctcgag cacgccgtag gcaaagggag accccgaccc cgtggcgacg atctcctctt
     1981 ccttggtcgc cccgcccagc gcatcgacgg agtagacgct cggtccctcg tcgtcgaacc
     2041 cgcccatcag gagctggacg tagtagggat aataccggtt ctggttcaag tagttcgaga
     2101 gcagggtcga tgccgcgccg accgacatgg tcttgccgcg gcgcatctca aagaggttgc
     2161 actcgacctg gaggatccgg acgagttgct gcgcgtcgcc gacgccgccg gcggtggtca
     2221 tgccgatccg cggcgtgatc tggtagatct tcttggcacg cttgctcgcg atcatgttgc
     2281 ccatcgtcgc ccgcatctcg gtggcaagaa ccacgccctc gttgaagatc agtcccaccg
     2341 tggttgtacc cttcataatt tcctgggaag tatcaagcat tgaagcacct ctaaaacctt
     2401 taaagacgtt tgaatcgctc aaaagcggtc aaacgtatac agcgatatca taaaacataa
     2461 atttatcgtc gtaccgggtt tcagcacgat acgataagca tcactatgtt atataatgtt
     2521 gccccttaat tctttcgcaa gcccggctgg tccggcagca gggccttgat cagctcccgg
     2581 tcgaagagca tggcgatgag tcttttgttc ccgttgacca ccgggagctg gtcgacccgg
     2641 gcccgccgca tcttcagtgc gcattcgccg acctcggcgt tgatggggac ggcaaggacg
     2701 tttcggacca tagcgttctt gacggggatg gggaggagct ggatccgcga gatcccgtag
     2761 ctgatggtgt gcatatccct gatgctctcc caggtccact cgtcgtcgtc cgtgccgttc
     2821 gagaagtcgc tgacctcgac gccgtcctcg atgcgggtgt gccggatgag gtcgcgctcc
     2881 gatatgatgc cggtgagcgt gccgtcctcc atcaggatag ggatggcatc gtagcccgat
     2941 atctcaagaa ctcgcccgac gagcgagagg ggcgtctcct cccagagtgc gtaggtcttg
     3001 ctgatgtagt tgtcccggat cggcaccgcc aggcgcatct gggcgatggc gccgatgagg
     3061 tcggcgacgc tgatgatacc gaccatggac ccgttctcca cgaccgggag cctccggatg
     3121 ttgtgccgga ccatcaactg cgccgcttca gggaggggag cgtccggtct gatgacgacc
     3181 gggtcgggcg tcatcagaag tccgagctgg gtctcttccg ccttgcggag gagatctttc
     3241 cgggtgatga tcccgacgag ctcgccgccc tttaagaccg gcacgccgct gatgcccgtg
     3301 cgtttcagta tgcgcaggac atcgtc
//
LOCUS       DAOSII010000197         3325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_25031_length_3325_cov_49.738532, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000197 DAOSII010000000
VERSION     DAOSII010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25031_length_3325_cov_49.738532"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1445)
                     /gene="malQ"
                     /locus_tag="PLV90_10095"
     CDS             complement(<1..1445)
                     /gene="malQ"
                     /locus_tag="PLV90_10095"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOF97506.1"
                     /translation="MNRRGSGVLLHITSLPSAYGIGDLGPAARRFVDLLAGTGQRYWQ
                     ILPLNPTCPAFGNSPYQSRSAFAGNTWLISPEQMVADGFLAPEDIRDPPPFPAERVDY
                     QAVLDYKNSLFDRAFARFKEHGENFRYMDFAAENAMWLNDYAIFVALKDRFGGKVWGD
                     WPVGIRDRHDEALRKHGAELAEPILREKFLQYIFDHQWRVLKNHCRDRHIQIIGDIPI
                     YLTYDSVDLWANPELFKLDEHKKPTVISGVPPDMFSATGQLWGNPIYNWEMHQKQEFR
                     WWESRIDHTLAFVDRVRLDHFRGFVQFWEVPGGDVTAQNGHWADAPGRDLFTLLTRGR
                     SCLPFIAEDLGYITPDVHEMMAHFGFPGMKILVFGFSGDVAQNPHAPHNITSGCVAYT
                     GTHDNNTVRGWFEHEVSDDQKNLIFSYIGGSVSADHIHRILIRLAMISPAGTVIVPMQ
                     DILGLGEEARMNRPGTAEGNWEWRLLPEQAT"
     gene            complement(1442..>3325)
                     /locus_tag="PLV90_10100"
     CDS             complement(1442..>3325)
                     /locus_tag="PLV90_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HOF97507.1"
                     /translation="AGIRFTILAPHQAGRVRAIGADEWTDVSGGRVETRMPYICRLPS
                     GRSIAIFFYDGTIARDVAFGDLLADGRRFAGRLFGAFSPGRRRPELVHIATDGETYGH
                     HRKFGEMALAYCLRAVEARRDVRLTVYGEYLAAHPPTHEVAVREGTSWSCTHGIGRWQ
                     EGCGCHCGTHPDWSQAWRRPLREAIVMVRDALAPRSAEALATLVRDPRETREDAVDLA
                     LGGWSDETVERFLSRHALQGISPGERRRVLALLEMERQVMLMQTSCGWFFDDIAEPGS
                     VQVMRHAGRAMDLGRQALGIDLEPAFIGILRRAPSNDPGYATGAAVYEVLVRPAAADP
                     ARIGAGAALYEIFGITPLAAESGMYAIRGDWPYRSETGGRQNLLGTVRVRSRVTGEEV
                     FLDAVASFSGIDRVVIGVREPGGGEALRAAWEEVMGTGEIGAFTRVYTASDLSDEERW
                     TIARRVLPGIGSAVCAVYRDAAPLLGVLEDLGIPEPGVATLLREHACTERLLAILESG
                     CPDPDRFAALAADVRAGNFRLDLPTLGEAASRRIAASVRAAAARPEDPMPLEEIARIV
                     RCAKVFGLPLTLWESQNACIGMRRQYAVMQQRAGRGDGDAERWAGAFRRAAACLGVRG
                     CEV"
BASE COUNT          549 a         1131 c         1073 g          572 t
ORIGIN      
        1 tcggttgcct gttcgggcag aagacgccac tcccagtttc cctccgctgt gcccggccgg
       61 ttcatccgcg cctcctcgcc gaggccgaga atatcctgca tcgggacgat cacggtcccg
      121 gcgggggaga tcatcgcgag ccggatgagg atccggtgta tatggtcggc gctgaccgat
      181 cccccgatat agctaaatat gagattcttc tggtcgtcgg agacctcgtg ctcgaaccac
      241 cccctgaccg tgttgttgtc gtgcgtcccc gtgtaggcga cgcacccgct cgtgatgttg
      301 tggggcgcgt gagggttctg tgcgacgtcg ccggagaacc cgaataccag gatcttcatg
      361 ccgggaaacc cgaagtgtgc catcatctcg tggacatcgg gggtgatgta gccgaggtct
      421 tcggcgatga acgggaggca cgagcggccc cgtgtaagca gggtgaagag gtcccggccg
      481 ggcgcatcgg cccagtgccc gttctgtgca gtcacatccc cgcccggcac ctcccagaac
      541 tgcacgaatc cccggaagtg atcgaggcgc acccggtcga cgaaggcgag ggtgtggtcg
      601 atccggctct cccaccaccg gaactcctgc ttctggtgca tctcccagtt gtagatcggg
      661 tttccccaga gttgcccggt cgcgctgaac atatccgggg ggacgcccga gatgaccgtc
      721 ggtttcttgt gttcatccag tttgaagagt tccgggttcg cccagaggtc gacgctgtcg
      781 taggtgaggt agatcgggat gtcgccgatg atctggatgt gacggtcacg gcaatggttc
      841 ttgagtacgc gccactggtg gtcgaagata tactggagga acttctcccg gaggataggc
      901 tccgccagtt ccgcgccgtg tttccgcaat gcctcatcgt gtcggtcccg gatcccgacc
      961 ggccagtcgc cccagacctt cccgccgaac cggtccttga gggcgacaaa gatggcgtaa
     1021 tcgttgagcc acatggcgtt ttctgccgca aaatccatgt aacggaagtt ctccccgtgc
     1081 tctttgaatc gtgcaaacgc acggtcaaag agactgttct tgtagtccag gaccgcctgg
     1141 taatccaccc tctccgcggg gaagggcgga gggtcgcgga tatcctccgg ggcgaggaag
     1201 ccatcggcga ccatctgctc ggggctgatg agccaggtat tcccggcaaa cgccgatcta
     1261 ctctggtagg gcgagttgcc gaacgcgggg cacgtcgggt tgaggggaag gatctgccag
     1321 tagcgctgtc ctgtcccggc aagaaggtcc acgaaccggc gtgccgctgg cccgaggtcg
     1381 ccgatgccgt atgccgatgg cagggacgtg atatgcagga gtaccccgct tcctcgccgg
     1441 ttcacacctc acaccccctc acgccaaggc atgccgccgc cctcctgaag gcccccgccc
     1501 atcgctccgc atccccgtca ccccggccgg cacgctgttg catcacggca tactgcctgc
     1561 gcataccgat gcaggcgttc tggctctccc atagagtgag tgggaggcca aaaactttcg
     1621 cacaccggac gatgcgggcg atctcctcaa gcggcatcgg atcttccggc cgggccgccg
     1681 ccgccctgac ggaggccgct attcgcctgc tcgccgcctc cccgagtgtc gggagatcga
     1741 gcctgaagtt tcctgcccgc acatccgcgg cgagtgccgc aaagcgatcc gggtcggggc
     1801 atccgctctc aaggatggca agcagccgtt cggtgcaggc atgctcccgg agcagggttg
     1861 cgaccccggg ttcggggatc ccgaggtctt cgagtacgcc aagcagcggt gccgcatcac
     1921 ggtagaccgc gcagacggcc gacccgatgc cggggagaac ccggcgggcg atcgtccacc
     1981 gttcctcgtc ggagaggtcg gatgcggtgt aaacccgggt aaacgcccct atctctccgg
     2041 tccccatcac ctcttcccac gccgcccgga gcgcctcgcc gccaccgggc tcgcgcacgc
     2101 cgatgacgac ccggtctatg ccagaaaaag atgcgaccgc atccaagaac acctcttctc
     2161 ccgtcacccg ggaccgcacc cggacggtcc cgaggaggtt ctgccggccg ccggtctccg
     2221 accggtacgg ccagtccccg cggatggcgt acatccctga ttcggccgcg aggggggtga
     2281 tgccgaagat ctcgtagagt gcggcccccg cacctattcg ggcagggtcc gccgctgccg
     2341 gcctgaccag gacctcgtag accgcagcgc cggtcgcgta tcccggatcg tttgacggag
     2401 cacggcggag gataccgatg aacgccggtt cgagatcgat cccgagggcc tgtcggccga
     2461 gatccatcgc acggcccgca tgccgcatca cctggacgct ccccggttct gcgatgtcgt
     2521 cgaagaacca gccgcaactc gtctgcatca gcatcacctg ccgctccatc tcgagaagcg
     2581 cgagcacccg ccgccgttct ccgggcgata tgccctggag cgcgtgccgt gacaggaatc
     2641 tttccacggt ctcatccgac cacccgccga gggcgagatc gacggcatct tcccgggtct
     2701 cccgtgggtc tctgacgagg gttgcgagcg cctccgccga ccggggagca agggcgtcgc
     2761 ggaccatgac gatcgcctcc cggagcggcc tgcgccacgc ctgcgaccag tcggggtgcg
     2821 tcccgcagtg gcacccgcac ccctcctgcc agcgcccgat gccgtgggtg cagctccagg
     2881 atgttccttc ccggacggcg acctcgtgcg tgggcgggtg ggcggcgagg tactcgccgt
     2941 agaccgtgag gcggacgtca cggcgcgcct cgactgcccg gagacagtag gcgagcgcca
     3001 tctccccgaa cttgcggtgg tggccgtagg tctccccgtc ggtcgcgata tgcacgagtt
     3061 cggggcgtct cctgcccggg gagaacgcgc cgaacaaccg cccggcaaac cgccgcccgt
     3121 cggcgagcag gtccccgaac gcgacgtcgc gggcgatcgt cccgtcgtag aagaagatgg
     3181 cgatgctccg gcccgagggg agacggcaga tgtagggcat ccgtgtctcg acccggccgc
     3241 cggagacgtc cgtccactcg tccgccccga tcgcccgcac cctccctgcc tggtgggggg
     3301 caaggatcgt gaaccggatc ccggc
//
LOCUS       DAOSII010000198         3276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_25514_length_3276_cov_63.433716, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000198 DAOSII010000000
VERSION     DAOSII010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25514_length_3276_cov_63.433716"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="PLV90_10105"
     CDS             <1..1131
                     /locus_tag="PLV90_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOF97508.1"
                     /translation="NTMAGVLLSLQALLLAWFEPVVIASAMFAALVTHTFVDCVPGTF
                     LGIPDADTSLAVLPAHSLCLEGRGEEAVRISALGSAAGAALSLPLAVAFVLVLPALQP
                     GIDWGIGLIILAVGGYLIVVSESPGWALAVFSVSGMLGLFSLNYSFLAWPTGGESGVL
                     MPLLSGLFGIAVLLRASHGLMPAQHFTGIDLLPRAIRRGSILGSLAGALVGWLPGLSN
                     ATANALLTSVIGYDSNPREYILATSAANTVNAFLGLAAFYAISRTRNGVMAALAAAEE
                     IPPATALLLAGALAAVGAYLLTLLLSRAAWLFGDVNVTRLNQGVIAFVVLLSLFLCGP
                     FGGLVLVLATAVGCVPSLVNIRRVYCMGAIMVPVMAYSFGLA"
     gene            1165..2025
                     /locus_tag="PLV90_10110"
     CDS             1165..2025
                     /locus_tag="PLV90_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-processing protein"
                     /protein_id="HOF97509.1"
                     /translation="MLQRYWFGDVDEQGCRAAGTDPAALAERAAALRTGMESYVPIDW
                     EVARDCGVARTREEYVDLLRAVCITLAREKIAVSYRARDVELLQMVRMLDELDNVINL
                     LSERSAEWYQVTTPSFSRKYRSLPAKKMLDLVRRGARGGLSDVAGDIERLAGTRSRLM
                     REVSARADEVLPNTSALIGGLVAARLLSRAGGLSALARMPGSAIQVLGSERALFSHLR
                     GGTPPPKHGIIFQHRRVHNAPREVRGRVARVLAAKLAIAARLDYYRGEAVPEFIRDAQ
                     EKIDGAGVAA"
     gene            2022..2624
                     /locus_tag="PLV90_10115"
     CDS             2022..2624
                     /locus_tag="PLV90_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrillarin-like rRNA/tRNA
                     2'-O-methyltransferase"
                     /protein_id="HOF97510.1"
                     /translation="MIRLGNVLVSPGEGGVYGERTLDGYRVWDPYRSKLAALYTLGGG
                     VDLTPDMRVLYLGAANGTTVSHVADYAGTVYAVEFAPRPMQDLLEVARRRKKIVPIMA
                     DAGRPEEYAPFMEAVDLIYQDVAQPDQVGIAERNLVFLKPGGHFILMLKTRSVDVRRD
                     PVEVLGEARAGLGRRLDVVDVRWLEPYHHDHAAIVCSRRG"
     gene            2654..>3276
                     /locus_tag="PLV90_10120"
     CDS             2654..>3276
                     /locus_tag="PLV90_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HOF97511.1"
                     /translation="MDRVVFNPFAPLMLILLFGLLVLVGLVIILFPIVFLTAIGATFT
                     KLGFSWREALLILLLTLAGSFVNVPVRTLESRAAPAYDRYVAVYGRLYRIPEPVQRTV
                     LAVNVGGALIPLAISLYLLYDSVVISGGYLVMGLALAGVAVVTVVTKLVARPVPGLGI
                     ATPFFIPPLAALIAALVLSTFAGGVPAAAVIIAYVSGTLGTLIGADLL"
BASE COUNT          421 a         1137 c         1097 g          621 t
ORIGIN      
        1 aacaccatgg cgggcgtcct cctctcgctc caggcgctcc tgctcgcctg gttcgaaccg
       61 gtggtcatcg cgtcggccat gtttgccgcg ctcgtcacgc atacgttcgt cgactgcgtc
      121 cccgggacgt tcctcggcat ccccgatgcc gatacgtcgc ttgcggtcct ccccgcgcac
      181 tccctctgcc tcgaggggcg gggggaggag gccgtcagga tatcggccct cgggagcgcc
      241 gccggcgccg ctctctccct cccgctcgcg gtggcgttcg tcctggtcct ccccgcgctc
      301 cagccgggga tcgactgggg catcggcctc atcatactcg cggttggagg ttacctcatc
      361 gtcgtctccg agtcgccggg gtgggcgctc gcggtcttct cggtctccgg gatgctcggc
      421 ctcttctccc tcaactactc cttcctcgcc tggccgacgg gcggtgagtc cggggtgctg
      481 atgcccctcc tctcggggct cttcgggatc gcggtcctcc tccgggcgtc gcacgggctc
      541 atgcccgcac agcacttcac ggggatcgat ctcttgcccc gggcaatccg gcgcggatcc
      601 attctcggtt ctctcgcggg cgctctcgtc ggctggctcc ccggcctctc gaacgccacc
      661 gcaaacgctc tcctgacatc ggtcatcggc tacgactcca acccgcgcga gtatatcctt
      721 gcaaccagcg ctgcgaacac cgtgaacgcc ttcctcgggc ttgcggcgtt ctacgccata
      781 tcccggacgc gaaacggggt gatggcggcg ctcgcggcgg cggaggagat cccgccggcc
      841 accgccctcc tccttgcggg cgcactggcc gcggtcgggg cctacctcct gaccctcctc
      901 ctctcgcggg cggcctggtt gtttggagac gtgaacgtca cgcggttgaa ccagggcgtc
      961 atcgccttcg tcgtcctgct ctccctcttc ctctgcgggc cgttcggggg actggtcctc
     1021 gtgcttgcaa cggcggtcgg ctgcgtcccg tctctcgtca acatccgccg ggtctactgc
     1081 atgggggcga tcatggtccc ggtgatggcc tattcgttcg gtcttgcctg acctcttata
     1141 tcggcgaact tcctatacca taccatgcta cagcgctact ggttcgggga cgtcgacgag
     1201 caggggtgcc gggcggccgg caccgatccg gccgcgctcg cagagcgggc ggccgcgctc
     1261 cgcaccggta tggagtcgta cgttcccatc gactgggagg ttgcccggga ctgcggggtc
     1321 gcccggaccc gggaggagta cgtcgacctg ctccgcgcgg tctgcatcac gcttgcccgg
     1381 gagaagatcg cggtctccta ccgggcccgg gacgtcgagc tcctccagat ggtcaggatg
     1441 ctcgacgaac tcgacaacgt catcaacctc ctctcggagc gttctgcgga gtggtaccag
     1501 gtcacgacgc cgtcgttctc gcggaagtac cgcagcctcc cggcaaagaa gatgctcgac
     1561 ctcgtccggc ggggtgcccg gggcggcctt tcggatgtcg ccggcgatat cgagcggctt
     1621 gccgggacga ggagccgcct gatgcgggag gtctcggccc gggccgacga ggtgctgccg
     1681 aacacgagcg ccctcatcgg cggcctggtc gcggcccgtc tcctctcccg ggcgggggga
     1741 ctttcggcgc tcgcgaggat gccggggagc gccatccagg tcctcggatc ggagcgggcg
     1801 ctcttttcgc acctgcgggg cgggacgccg ccgccgaagc acgggatcat attccagcac
     1861 cgccgggtcc acaacgcgcc ccgcgaggtc cgcggaaggg tggcccgggt gctcgcggcg
     1921 aagcttgcta tcgccgcccg gctcgactac taccgggggg aggcggtgcc ggagttcatc
     1981 agggatgcgc aggaaaagat cgacggggcg ggggtcgcgg catgatccga ctcgggaacg
     2041 ttctcgtctc gcccggggag ggcggggtct acggggagcg gacgctcgac ggctaccggg
     2101 tctgggatcc ctaccggagc aagcttgcgg ctctctatac gctcggcggc ggggtggatc
     2161 tcacacccga tatgagggtg ctctacctcg gtgcggcgaa cgggaccacg gtctcgcacg
     2221 tcgccgacta cgccgggacc gtctatgcag tggagtttgc accccggccg atgcaggacc
     2281 tcctcgaggt ggcccgccgg aggaagaaga tcgtcccgat catggccgac gcaggccggc
     2341 cggaggagta tgcgccgttc atggaggcgg tcgacctgat ctaccaggat gtggcgcagc
     2401 cggaccaggt cgggatagcg gagcgaaacc tggtcttcct caagcccgga gggcacttta
     2461 tactgatgct caagacccgg agcgtggatg tccggcggga cccggtcgaa gtgctcggcg
     2521 aggcgcgggc cggccttgga cgacgcctcg acgtggtgga tgtccggtgg cttgagccct
     2581 accaccacga ccacgccgcg atcgtctgct cccgccgggg ataatatata gtagctgggg
     2641 gcgaactctt cgtatggatc gggtcgtctt caaccccttt gctcccctga tgctcattct
     2701 cctcttcggg ctgctcgtac ttgtcgggct cgtcatcatt ctctttccga tcgtctttct
     2761 gacggcgatc ggggcgacgt tcacgaagct cgggttctcc tggcgggagg cgctcctgat
     2821 ccttctcctg acgcttgccg ggagtttcgt caacgtaccg gtcaggacgc tcgagagccg
     2881 cgccgcgccg gcgtacgacc gctacgtcgc cgtgtacggc cggctctacc ggatccccga
     2941 gcccgtgcag cggacggtcc tcgcggtcaa cgtcggcggt gccctcattc cgctcgcgat
     3001 atcgctctac ctcctctacg actcggtcgt gatcagcggg ggctacctgg tcatggggct
     3061 cgccctcgcc ggcgtggcgg tcgtgaccgt cgtgacgaag ctcgtcgccc gcccggtgcc
     3121 gggcctcggt atcgcaacgc cgttcttcat accgccgctt gcggcgctca tcgcggcgct
     3181 cgtcctctcg acgtttgcgg ggggcgtccc ggccgcggcc gtgatcatcg cctacgtgag
     3241 cgggacgctc ggcaccctga tcggggcgga cctcct
//
LOCUS       DAOSII010000199         3273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_25541_length_3273_cov_53.624301, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000199 DAOSII010000000
VERSION     DAOSII010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25541_length_3273_cov_53.624301"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="PLV90_10125"
     CDS             complement(<1..889)
                     /locus_tag="PLV90_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOF97512.1"
                     /translation="MDAGVIGVGMMGRNHARVYSELKAVDSLYLYDLNEKATRDLAGA
                     FEAAASPSLEDLLRSVDVVSICVPTPYHFGVAETVIGAGVPFLVEKPICATAEETRRL
                     IAKIPDGFAAGVGHIERFNPIVPEIKKIVRDPLYIEMKRHNPASSRVSGSSVVEDLMI
                     HDVDIMRNVLLPDGAYRLAGSGNQDVCSALFSFGGTPVYLSASRKSSKKIRMIYIEEE
                     EFTVEGDFMAQEIYIHRKPGQYSVENERYVQENIIEKVLVNKQEPLKLELSTFLDCVA
                     RGREFPVSPAQALLNMEICE"
     gene            1087..2571
                     /locus_tag="PLV90_10130"
     CDS             1087..2571
                     /locus_tag="PLV90_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HOF97513.1"
                     /translation="MLVFRPSRYLARVMRVDPVQRQSLISFGSTIGLTAIGFLSTMFF
                     THTVGLSILGAYFLFVAYFSVFNLIGDGGFGGAAVKRISEGEDQNAYFTAFTLLRVAL
                     TVVSVGFLLIAQPYLVKLTSAGVFPWLILALVVSTFTNIASNSVYGRGMVGVNQIGSL
                     IDTLARILVQVAAVVLGYGVAGLAGGFVAGILASGLFNLRFLDLSPAPFRRSHLKSLF
                     TFSVWTFLSSSGYLVFSYADTIMIGHFMTDADVGVYRVALQLTSIATFVTIALHTTLY
                     PKVSYWGKQGDLSSVERALARAFTYSLLLAVPVVAGGWLLGERLLYFFYTASATAGAG
                     ALAILLLVQVAHVFMFLQTMCLNALDRPRDSFRVTAIAVAANIGLNLLLIPAYGIVGA
                     SAATLVTMILNAVLAHRALSRSIRIRIEPRAVGHIVLAALVMAAVVVVCTFIIPLSNV
                     FVTLGAVGLGGLVYILALLRLDEGIHDELKDLAGQLGIPWPGVL"
     gene            2774..3211
                     /locus_tag="PLV90_10135"
     CDS             2774..3211
                     /locus_tag="PLV90_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF97514.1"
                     /translation="MKKEIRELEHEEFPLAEKVWTHYRDQKADPRRERIFGVFVDGVL
                     AATARCTRHPGGLEMDCVFTLDEYRGGGYAREAVEMLLDECGSETIYIHSTLPLVGFY
                     GKLGFVPIPEASLPASIKERFVFCFGEMAGCNVAPMMRNPPSG"
BASE COUNT          559 a         1076 c          948 g          690 t
ORIGIN      
        1 cctcgcagat ctccatattc agcagcgcct gcgcggggct gaccgggaac tcccggccgc
       61 gcgccacgca gtcgaggaac gtcgagagtt cgagtttcag cggctcctgt ttgttcacga
      121 gcaccttctc gatgatgttc tcctggacgt aacgctcgtt ctctaccgaa tactgtccgg
      181 gttttctatg gatgtagatc tcctgggcca tgaagtcccc ctcgacggtg aactcctcct
      241 cctcgatgta gatcatgcgg atcttcttgg atgatttcct gcttgccgag aggtagaccg
      301 gggttccccc aaaagagaag agggcgctac acacatcctg gttgccgctg ccggcaaggc
      361 gatatgcccc gtccgggagg aggacgtttc tcatgatatc cacatcgtgg atcatcaggt
      421 cctcgacgac cgaagagccg ctcacccggg aagaggccgg gttgtgccgc ttcatctcga
      481 tatagagggg gtcgcggacg atcttcttga tctcgggaac gatcgggttg aaccgctcga
      541 tgtgcccgac gccggcggcg aagccatccg ggatcttcgc aataagcctc cgcgtctctt
      601 ccgccgtcgc gcatatgggt ttctcgacga ggaacggcac cccggcaccg atgaccgtct
      661 ctgcaacccc gaagtgatag ggcgtcggga cacagatgct caccacatcc acagatcgaa
      721 ggagatcctc gagagacggg gaggctgctg cctcgaaagc tccggcaagg tcgcgagtcg
      781 ccttctcatt gaggtcgtac agatagagtg agtctaccgc tttcaattcc gaatagacac
      841 gggcatggtt tctgcccatc atcccgacac cgattacccc tgcatccaaa ttatcaccgc
      901 cgacggtatt gccacgtatg ctcatccggg aatcccggat ggtaataata ctacacattg
      961 gcatcgctct ctcatgacgt tatcgtgggg aggtatcccc tcgctccgga atgccggggt
     1021 ccacaggatc acgaccgcct ctcctgctcc ggttcaaaat gaataaggtt cttcacggcc
     1081 aaaccaatgc tcgtgtttcg gccctcccgg tatcttgccc gtgtcatgcg cgtggaccct
     1141 gtccagcgcc agagcctcat cagcttcggt tcaacgatcg ggcttactgc gatcggattt
     1201 ctttccacga tgttcttcac ccataccgtc ggtctatcta tcctgggcgc atattttctc
     1261 tttgtcgcct acttcagcgt cttcaacctg atcggggacg gcggatttgg gggcgccgca
     1321 gtgaagcgga tcagcgaagg agaggaccag aacgcctact tcaccgcgtt tacccttctc
     1381 cgggtggcgc tgacggtcgt ctcggtgggt ttcctcctca tcgcgcagcc gtacctcgtc
     1441 aaactcacct cggcaggagt cttcccctgg ctgatactcg ctctggtcgt cagcaccttc
     1501 acgaacatcg cctcaaacag cgtttacggg agaggaatgg tcggcgtcaa ccagatcggc
     1561 agcctgatcg acaccctggc ccggatactc gtccaggtcg ccgcggtcgt cctcggctac
     1621 ggggttgccg gcctcgccgg cgggttcgtt gccggcattc tggcttcagg gctcttcaac
     1681 ctccgtttcc tcgacctctc cccggccccg tttcgccggt cgcacctcaa gagcctcttt
     1741 accttctcgg tctggacgtt cctcagctcg agcggctacc ttgtcttttc gtatgccgac
     1801 accatcatga tcggtcactt catgaccgat gccgacgtcg gcgtctaccg cgtagcgctc
     1861 cagctcacgt cgatcgcgac gttcgtcacc attgcgctcc ataccaccct ctatcctaag
     1921 gtcagttact ggggaaaaca gggcgacctc tcctcagtag agcgcgcgct cgcccgtgct
     1981 ttcacctact cgctcctgct cgcggtcccg gtcgttgccg ggggctggct cctcggggag
     2041 cgtctcctct acttcttcta cacggcatcg gccaccgccg gggcgggagc gctcgcgatc
     2101 ctcctgctcg tccaggtggc ccatgtcttc atgttcctcc agacgatgtg cttaaacgcc
     2161 ctcgaccgac cgcgcgactc gttccgggtg acggcgatcg cggttgccgc aaacatcggg
     2221 ctaaacctcc tcctcatacc ggcatacggc atcgtcgggg cgtcggcggc gacgctcgtc
     2281 accatgatac tgaatgccgt actcgcccat cgcgccctct cccggagcat ccgtatccgg
     2341 atagaacccc gggccgtggg tcatatcgtc cttgccgcac tcgtcatggc ggccgtcgtc
     2401 gtggtctgca cgttcatcat cccgctctcg aacgtctttg tcaccctcgg agcggtcgga
     2461 ctcggcggac tggtctacat cctggccctc ctcaggctgg atgagggtat ccacgacgag
     2521 ctgaaggatc ttgccggaca actcggcatc ccctggcccg gtgtgctctg agggccggcg
     2581 gggcactctg ccggctgaga atcccggggc gaggtgaggc ggcggtgcat ccgctcccgg
     2641 ttgccgggtg cccgcctcct ccccgttcca ggatgcctcc tgcaggaagg cggtccgggt
     2701 accggtcgga tagtcaggaa tcgatggggc agtcgacttg aaataatagg cgcggcaaca
     2761 tgggccacac gtgatgaaga aagagatccg cgaactggag cacgaagagt tcccgcttgc
     2821 ggagaaggtc tggacacact accgcgacca gaaggcggac ccgcgccggg agaggatctt
     2881 cggcgtcttt gtcgacggtg tcctggcggc gacggcccgc tgcacccggc acccgggcgg
     2941 cctcgagatg gactgcgtct tcacgctcga cgaatatcgt ggaggcgggt atgcgcggga
     3001 ggccgtagag atgctcctcg acgaatgcgg ctccgaaacg atctacatcc actcgaccct
     3061 ccccctggtc gggttctacg ggaaactcgg gtttgtgccc atcccggagg cgagcctgcc
     3121 ggcgagcatc aaagagcggt tcgtcttctg cttcggggag atggcggggt gcaacgtcgc
     3181 ccccatgatg cgcaaccccc cgagcgggta accccgctct cctgcttacc gcgcggcggc
     3241 cggccggggg aacgtatccg ccgccggacc ccc
//
LOCUS       DAOSII010000200         3262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_25680_length_3262_cov_50.116620, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000200 DAOSII010000000
VERSION     DAOSII010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25680_length_3262_cov_50.116620"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PLV90_10140"
     CDS             <1..542
                     /locus_tag="PLV90_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97515.1"
                     /translation="DGIPVLNISADRIDPVYTNRIVPIGIMPGQNESELPKPTHVYSN
                     RYSEETPVLVDMELHLPGISPDHDIETRAPIGVEPLFMPCRVIGNLDGSGGFVDKNHY
                     VSPGSTGYIVEVPFTLSFDADDENVLTISSGFLGTNQRWILGWQSNSYSESLRHEFKG
                     PCNGTYPTRGILLTGKGVY"
     gene            646..1497
                     /locus_tag="PLV90_10145"
     CDS             646..1497
                     /locus_tag="PLV90_10145"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOF97516.1"
                     /translation="MGELTQDLRDQRDAWDEANERHRGNPLRSVVSVDDLNTMYIDGS
                     QKRLVSRAVRLEPHFTVLDYGCGVGRWTLWFAPEVHRVVGVDLSPKMVEAARRMAGNT
                     GIQNAEHHTIEGMPFPFEDDAFDLVNAVWVLRYIIDDDELARTVEEICRVVRPGGHVT
                     FIEMIAKKEPEFKEHEGVFTGAAVYRRLEQYRSLFEGCGMMMKMSAVSSASPLYWLYA
                     LIRDAAKRRSLPDPFSPLAPSIVSASLATESLTATPMRLLADRGLIRCRHRFLCLQKP
                     GGDRTRS"
     gene            complement(1543..>1975)
                     /locus_tag="PLV90_10150"
     CDS             complement(1543..>1975)
                     /locus_tag="PLV90_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOF97517.1"
                     /translation="GSGRFAAPLGIPIGIEPSPALGRMARERGIEVIRGRAESIPLRD
                     GACSSALVVTVICFLDDPVPAFREIHRILAPQGTLILGFLEREGPVAQKYLHEEGKHR
                     FLSRARFYSPDEVRELLGRTGFCVAGAESQAGFSVFAARKA"
     assembly_gap    1976..2075
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2180..2548)
                     /locus_tag="PLV90_10155"
     CDS             complement(2180..2548)
                     /locus_tag="PLV90_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97518.1"
                     /translation="MRILRSVRHKSCADGSFMKEFLLDAPVTAGFFAYLENFGEVEAL
                     RNVGEGFYKFEKTDWFSIKGMNGDTTVEVRFKKEAMDLTADFVYLLFSSYREGAADLS
                     LLKQREQAIERRVKERLYGP"
     gene            complement(2520..>3262)
                     /locus_tag="PLV90_10160"
     CDS             complement(2520..>3262)
                     /locus_tag="PLV90_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOF97519.1"
                     /translation="TRLMVTAHGSNVLGSVQEIARIGKILHDHDISFIVDGAQTAGHI
                     PVDLWSLPVDAYVFTGHKGLFGIPGTGGFYVRDPETIAPTRYGGTGTDSFSLFQPRDM
                     PERFESGTHNYPGLAALAAGAEYIAGIGVETIAEKAERQTAFIIGELKEEPNVTVYNE
                     SPSLPIVSFNIRGMENDDVGFILARAYGIVARTGLHCAPLAHRAIDGGRGSVRLSLSW
                     FTTDEECRVAARAIREIAQHADPSVGSP"
BASE COUNT          609 a         1004 c          981 g          568 t
ORIGIN      
        1 tcgacggcat ccccgtgctg aacatatcgg ccgaccggat cgatccggtc tatacgaaca
       61 ggatcgtgcc aatcgggatt atgccggggc agaacgagtc ggaactccca aaaccgaccc
      121 atgtttactc aaacaggtat tcggaagaga cgcccgtgct cgttgatatg gaactacacc
      181 tgcccggtat cagccccgat cacgatatag agacccgggc gccgataggg gtcgaacccc
      241 tgttcatgcc ctgccgggtt atcggcaacc ttgacgggtc cggcggattc gtcgacaaaa
      301 accactacgt gagcccgggg tcaacaggct acatcgtgga ggtgccgttt accctctcgt
      361 ttgatgccga tgacgagaac gtcctcacca tctcgtccgg atttctggga acaaaccagc
      421 ggtggatcct cggctggcag tcgaattcgt actccgagag cctgcgccac gagttcaaag
      481 gcccgtgcaa cggcacttac ccgacaaggg gcatcctcct gacgggtaag ggcgtatact
      541 gacccgctct gctatttttg agtcggatag gatcataccg gccggacatg catcgcacag
      601 cagaaaccgt taagtgcctg tcaacggttc aaccccccca tcaagatggg cgagctcacg
      661 caggacctcc gggatcaacg cgatgcctgg gatgaagcaa acgagcggca tcgcggcaat
      721 ccgctcaggt cggtggtcag tgtcgatgac ctgaatacca tgtacatcga cggcagccag
      781 aagcgcctcg tctcaagagc ggtcaggctg gaaccgcatt ttacggtgct cgattacggg
      841 tgcggagtcg gccggtggac gctctggttc gcgccggagg tgcatcgcgt tgtcggagtg
      901 gacctctcgc caaagatggt ggaagcggca cgacggatgg cgggcaatac cggcatacag
      961 aacgccgagc accataccat cgagggcatg ccgtttcctt tcgaggatga cgcgttcgac
     1021 ctcgtcaatg ccgtctgggt cctgcgctac atcatcgatg acgacgagct tgcgcgaacc
     1081 gtcgaggaga tctgccgcgt ggtccggccg ggaggccacg tcacgttcat cgagatgatc
     1141 gcgaagaaag aaccggagtt caaggagcac gagggtgttt tcaccggagc ggcggtatac
     1201 cggagactgg agcagtaccg atcgctcttt gagggatgcg gcatgatgat gaagatgagc
     1261 gcggtctcaa gcgcatcgcc cctctactgg ctgtatgccc tcatccgcga cgccgcaaag
     1321 cgccggagcc tgccggatcc gttctcgccc ctggccccct cgatcgtctc tgcaagcctc
     1381 gcgaccgaga gcctgacggc aacaccgatg cggctcctgg ccgaccgcgg cctcatccgg
     1441 tgcaggcacc gctttctctg tctccagaag ccggggggcg accgtacccg gtcataaggt
     1501 ttgcgagggg aggacgatac ccggtcccct cgcgattctc gatcaggcct tccgcgcagc
     1561 aaaaacggag aaaccggcct gcgactcagc cccggccaca caaaacccgg tgcgcccgag
     1621 gagttcccgg acttcgtccg gtgaataaaa ccgggcgcgg gagaggaacc ggtgcttccc
     1681 ctcctcgtgc aggtacttct gagcgaccgg tccctcacgc tcgagaaacc cgagtatgag
     1741 ggtcccctgc ggggcgagga tccggtgaat ctcgcgaaac gccgggaccg ggtcgtccag
     1801 gaagcagatg accgtcacca cgagcgcgga cgaacacgcc ccgtcccgga gcgggatcga
     1861 ctctgcccgc ccgcgaatga cctcgatccc tcgctctcgc gccatcctgc cgagggccgg
     1921 cgagggttcg atgccgatcg ggatgccgag gggcgcggca aaccgcccgg acccgnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcgcc ggacccgggc gagttctgcg
     2101 cggtactcgg cgcggtgctc gtcaaaccat cggtcataat cctccgcgaa ctgctcgaat
     2161 atatccccgg gcattccgat cacggcccat agaggcgctc ttttacccgc ctctcgatcg
     2221 cctgctcacg ctgtttgagg agggagaggt ccgccgctcc ttctcggtac gacgagaaga
     2281 gaaggtagac gaagtctgcc gtcaggtcca tcgcctcctt cttgaacctg acctcgaccg
     2341 tggtgtcgcc gttcattccc ttgatggaga accagtcggt cttctcgaac ttgtagaacc
     2401 cctcgccgac gttccggagt gcttcgacct ccccgaagtt ctcgaggtag gcaaagaacc
     2461 ccgcagttac cggcgcatcg aggaggaact ccttcatgaa ggagccgtcg gcacaggact
     2521 tatggcgaac cgaccgaagg atccgcatgc tgcgcaatct cccttatcgc ccgggctgca
     2581 acccggcatt cttcgtccgt cgtgaaccag gagaggctga gccgcacgga gcctctcccc
     2641 ccgtcgatcg ccctgtgcgc cagaggcgca cagtggagcc ccgtccgggc gacgatcccg
     2701 taggcccggg cgaggatgaa ccccacgtcg tcgttttcca tgccccggat gttgaacgag
     2761 acgatcggga gggaggggga ctcgttgtag accgttacgt tcggttcttc cttcaactcc
     2821 ccgatgatga acgccgtctg gcgctctgcc ttctctgcga tcgtctccac cccgatgccc
     2881 gcgatatact ccgctccggc cgcgagggcg gcaaggccgg ggtagttgtg ggtccctgac
     2941 tcgaaccgct ccggcatatc ccggggctga aagagcgaga aggagtccgt cccggtcccg
     3001 ccgtagcggg tgggggcgat tgtttccggg tcccgcacgt agaatccgcc ggtgcccggt
     3061 atgccgaaga ggcccttgtg accggtgaag acgtaggcgt cgaccgggag ggaccagagg
     3121 tcgacgggga tgtggccggc agtctgagcc ccgtcgacga tgaaggagat gtcgtgatcg
     3181 tgcaggatct tcccgatgcg ggcgatctcc tgcaccgacc cgagcacgtt gctcccgtgg
     3241 gccgtgacca tgagccgggt at
//
LOCUS       DAOSII010000201         3252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_25782_length_3252_cov_28.388176, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000201 DAOSII010000000
VERSION     DAOSII010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25782_length_3252_cov_28.388176"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..334
                     /locus_tag="PLV90_10165"
     CDS             101..334
                     /locus_tag="PLV90_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97520.1"
                     /translation="MLLRRIAELSQEQAGPLVSGAIHTAGEDTPKGGYTTFFQRLQKL
                     DVRLVDLVRVHAGGRTSEVVLRYPPEKVVQMCG"
     gene            complement(933..1490)
                     /locus_tag="PLV90_10170"
     CDS             complement(933..1490)
                     /locus_tag="PLV90_10170"
                     /inference="COORDINATES: protein motif:HMM:NF014028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97521.1"
                     /translation="MKPFASDDRAFTGLEAAMVFIAFIVVASIFAYMALGAGFFTTQK
                     AEQTVYSAVQQVGTVVQIVEPIMVQAGASGDSIRYIVIMVRGPQDADIDVEQIVVTVS
                     TAEAVQTYKGAAFWHVIGNGGQPPVFWSMRVPLYQADDPAIPGDLVISRNQRFFIEIK
                     SADAVPCAVERRAPAGMRPGSWYAV"
     gene            1663..2208
                     /locus_tag="PLV90_10175"
     CDS             1663..2208
                     /locus_tag="PLV90_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97522.1"
                     /translation="MEIRTIRGYLVALSIAGALLTAGCTSSLPSGDDQAYADAYPRSP
                     LRIVSPIRYQGAMANESGIVVTTLVLNLTVAPEAMDSGTPEHDREFNITPMYITYADN
                     RDRYTLGPGEYSLSRQGGRGGEVIELILPLRQPVPANTTIHAEFWIPYQGTLILSLRT
                     PDVIEPSGQVTEFAAAPFIPH"
     gene            complement(2195..2401)
                     /locus_tag="PLV90_10180"
     CDS             complement(2195..2401)
                     /locus_tag="PLV90_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97523.1"
                     /translation="MIPIDPLQGPAVVVPGMAGAVPAASGGTGADEQTGGGGYGASCT
                     AGVNGAARCLLNVSPEQNLTVSVG"
     gene            complement(2398..>3252)
                     /locus_tag="PLV90_10185"
     CDS             complement(2398..>3252)
                     /locus_tag="PLV90_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97524.1"
                     /translation="MGWEQFTIKMLQEALGLSYYQTYRILHGYTSRGTTYSGLLEKCP
                     AVSFFDTMVTEDVEGYAVRRREHLFSFDRTVYRRWAHGGGVWLDEDRDDGSDPRNPGR
                     PPDRSGGTARCDIAAGLPQDSGTGCNDKTAGNGAGSAGTCTDREKHLSLSTKVQQNTH
                     TVPGTGSGVGECAGVCEIRNAGTDPEIPAPRGEKSNPTPFNRPLAGIPGCNVLKRDAR
                     RGPALQEPLSGVLDHREFSRVRVELGRCDICDTGKAVYRSREARANLCEGCYARLVRE
                     GNAREGVR"
BASE COUNT          613 a         1043 c          965 g          631 t
ORIGIN      
        1 gagtcccggg cattgcattg cctctttcgt ccccaacgat ctttcctcca aacaggccag
       61 aaggcgcgct acttgttcgg ttacctccgc aggcgaacga atgctgctcc ggcggatcgc
      121 ggagctgtcg caggagcagg ccgggccgct ggtctccggc gcgatccata ctgcggggga
      181 ggatacgcca aagggcggtt acacgacctt cttccagcga ctgcaaaagc tcgacgtgcg
      241 gctggtcgac ctggtccggg tgcatgccgg cgggaggacg agcgaggtcg tgctgcgcta
      301 tccgccggag aaggtggtgc agatgtgcgg gtaggcgccg gcagttccgg tgaacccggt
      361 ctgggacgat aatcggtcgg tgggggctct ctgcaacggt ggagatcccc cacgagcaga
      421 ccggagggcg gtggaggtcg tttgaggggt ggcgcggtgc gaagcacggc gtgacgggca
      481 cttcccggca gaccatgacc tgctcgaacc tcgccgctca tggtcgtcag agatcccggg
      541 ccggaggaga agaacaggca gacgccacga tcaaagagag catggtctgc cggttggggg
      601 ttcccgattt tgggctgttg ccatgaatag ccctcttcgt gagaggagag cgcgaggagg
      661 ccattcacca tatcgtcgag aatatcccgc gggagacgct cctctgggta tacgaagagg
      721 cgtccagaga cccagctggc tgatactcca ccacttctct atcggcatcg atatccggaa
      781 cctgctccgc gccggtgggt tggcccggag cgacaccgcc ctggatcgtg agtgggggcc
      841 ggtcacgtcg gaggcggccc ggcgagtgca ggagaaagcg cagtgaaccc cttcagatac
      901 ccggcgagcg catccggggc ggacgactcg atctacaccg cataccagct gccgggtctc
      961 atgcctgccg gagccctgcg ctccaccgcg catggaacgg catcagccga ctttatctcg
     1021 atgaaaaacc tctggttccg gctgatgacc aggtcgcccg ggattgcagg gtcatcagcc
     1081 tgatatagcg gaacccgcat gctccagaag accggaggtt gcccgccgtt acctatgaca
     1141 tgccagaaag ccgccccttt gtacgtctgc acggcctcgg cggtcgacac ggtcacgacg
     1201 atctgctcta catcgatatc agcatcctgc ggtcctctca ccataatgac gatatagcgt
     1261 atgctgtcgc ctgaggcacc cgcctgcacc ataatgggtt cgacgatctg gacaaccgtg
     1321 ccgacctgct gcaccgccga gtaaaccgtc tgctctgctt tctgagtcgt gaagaacccc
     1381 gcaccgagcg ccatatacgc aaagatagag gctacgacaa taaaggcaat gaagaccatc
     1441 gctgcttcca gaccggtgaa tgctctgtca tcggatgcaa atggtttcat gctcgccgcc
     1501 gttcatacca gttagcgctg ttgctcgatt cctatgttta ctggattgat ggccgattat
     1561 ttgaatatac ggcacttgat atccggtgca accgacccgg ccacactcga aagtagacgt
     1621 accacatcag gtttttgatg ttaaactcct caacacttct ccatggagat acgcaccatt
     1681 cgtggatacc tggtagcact ctccatcgcc ggggccctcc tgactgccgg ttgcacttcg
     1741 tcgctgccat ccggcgacga tcaggcctat gcggacgcct accctcgctc tccgctcagg
     1801 atcgtatcac cgatccgcta tcaaggggcc atggcgaacg aatcgggcat agtagttacg
     1861 acgctcgtgc tcaatctaac ggttgccccg gaagcgatgg attccggcac tccagagcat
     1921 gaccgggagt tcaatatcac gccgatgtat atcacctacg cagataaccg tgaccgctac
     1981 acccttgggc cgggagagta ttccctctcc cggcagggag gcagaggtgg cgaggtcata
     2041 gagctgattc tccccctccg gcagcctgtt cccgcgaaca ccactatcca cgccgagttc
     2101 tggataccct accagggaac gctgatactt tcactccgca caccggacgt gatagaaccc
     2161 tccgggcagg tgacggagtt tgccgctgca ccctttatcc cacactgacg gtgaggttct
     2221 gttcgggcga gacgttgagc aggcatcggg ccgcgccgtt gaccccggcg gtgcaacttg
     2281 ctccgtatcc tcctcctccc gtctgttcat cggccccggt gccgccggat gccgccggca
     2341 cagccccggc cataccgggc accaccaccg ccggaccctg caggggatcg atggggatca
     2401 tcgcacccct tccctcgcgt tcccctcccg gaccagccgg gcgtagcacc cttcgcagag
     2461 gttcgcccgt gcctcacggg agcggtagac cgcttttccc gtatcgcaga tgtcgcaccg
     2521 gccaagctcg accctcaccc gggaaaactc ccggtggtcg agaacccccg atagaggctc
     2581 ttgcagcgca ggccctcgcc ttgcatctct tttcagcacg ttgcaaccgg ggatgccagc
     2641 cagagggcgg ttaaatggtg tcggatttga tttctcgccc cgtggtgccg gtatttcggg
     2701 atcggttccg gcattccgga tttcgcacac acccgcacac tcacccaccc cggaaccggt
     2761 accggggact gtgtgcgtgt tctgctgtac tttagtactg agagagagat gcttctctct
     2821 atctgtacag gtaccggcag atccggcgcc gttcccggcc gttttatcgt tgcagccggt
     2881 gccggaatcc tgcggtaaac ctgctgcaat gtcgcaacgg gcggtgcccc cgctccggtc
     2941 cggcggccgt cccgggttac gggggtcgga accgtcatcc cggtcctcgt ccagccagac
     3001 cccgccgccg tgggcccagc gccggtagac agtgcggtcg aaggagaaga gatgctcccg
     3061 ccgccgcacc gcgtaccctt ccacatcctc ggtgaccatc gtgtcgaaga aactgaccgc
     3121 cgggcacttc tccaggagac cggagtaagt cgtcccccgg ctcgtatagc cgtggaggat
     3181 acgataggtc tggtagtagg agagcccgag cgcctcctgg agcatcttga tcgtgaactg
     3241 ctcccagccc at
//
LOCUS       DAOSII010000202         3225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_26062_length_3225_cov_45.871924, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000202 DAOSII010000000
VERSION     DAOSII010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26062_length_3225_cov_45.871924"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(465..1895)
                     /locus_tag="PLV90_10190"
     CDS             complement(465..1895)
                     /locus_tag="PLV90_10190"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOF97525.1"
                     /translation="MKLLINGEWRDAASGKVFAVRNPATGDMLEEAPLGGEEDVRSAA
                     EAAGEAFADWSAKSPRARAKILFFAAEEVRRRNTDLAALLTAEQGKPIREAVDEINGF
                     ANILEYYYALSAGERGEFANLPGYGHTIVLRRPLGVCGAIIPWNMPAIIMGWKIGPAL
                     TTGNTMVLKPATTAPLTCGRLASILEGAGLPPGVLNVVTGPGETVGREIARNPEIRKV
                     SFTGEAGTGRQVALDAAPALKRVTLELGGSDPMIVCDDADIPMAVEGAVRGRFYNCGQ
                     TCTAVKRLYVFDSIADEFIRRLRSRVESIVVGNGMDRGVDMGPLNNPAGLERVVREVD
                     AVCERRDGEIVTGGQAPRGEGYERGFFFLPTLVAGLSHDSPLFSEEVFGPVLPVATVA
                     DLDEALIRANDSRYGLGASVWTRNADLIARATEGLEAGIVWVNQHLKIPPEVPFGGTK
                     GSGIGRENGSRALEAYTEEKVVMIRL"
     gene            complement(1905..2990)
                     /locus_tag="PLV90_10195"
     CDS             complement(1905..2990)
                     /locus_tag="PLV90_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF97526.1"
                     /translation="MPRSLPVLSGIFVVMALSNAVVPVLPGFAEGAAMQGAVYAAYFL
                     GAFIMVLPAGMASDRVGRVPLIRAGLFLTVASGVFMLLFPSGLPLLLFRAVEGIGAGL
                     FLSAALAWANSHPSAGRVSGYVMAAQNLGLVAGLLAAGWLDAVFDSHLAGIAFFTALA
                     IPALVMSAAIRESNHAGFVEADLSLIARNYFWLFTTAAVLVGMTGMVAALYPGYTGGD
                     PSLLALQLATVNIATVIAVLVVPRLDLRPAPAIRASSLLMAGAVAASYATPYALPVVG
                     ALAGVIIVAVLAFLAETGIRQGVMIGLFNTATYAGFTVLPALAGAIAGEFGFLAGFLL
                     PAGLAAAMAATIGRCPAQQSAYNRPHP"
     gene            complement(2993..>3225)
                     /locus_tag="PLV90_10200"
     CDS             complement(2993..>3225)
                     /locus_tag="PLV90_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HOF97527.1"
                     /translation="RETIVINLEIATSDGFSGHSDRKQLMNYVAHLQPRPEKIFTIHG
                     DENKTIDLASSIYKRHRIETHSPLNLETYRMI"
BASE COUNT          609 a         1101 c          989 g          526 t
ORIGIN      
        1 cggtcatgtt tccggttgtg ttcccggtca cgtttccggg cgtcatggtt gcattcccat
       61 ccccgaccag cgccgccgtc acggcgaacg ggatggccag aaggatgagg atgcacacgc
      121 atggagctat ccatcgtttc atgtcacttt ccccccttgg gagcgctaag ggcatgcatg
      181 taattatata taaatactgt tatcgttccg gcgacacctc caatagcaga acaaccgcgt
      241 aacgaactct cttttctctc gtagatcccc ggggagaggg ggcgaatccc cgggctcgcg
      301 caccccttcg cggccatcac ggcaacgtgc gcatgagccg gcagggtatg agcgatgagt
      361 gaacctctcg cgccggatca tctcctacac ccgggacccg cgcatgaaga cagcccggtg
      421 cagcagggcc cgggtggatg agaggccgcc ccggagggct ccgttcacaa ccgtatcatc
      481 accaccttct cctccgtata cgcttcaagc gcccggctgc cgttctccct cccgataccg
      541 ctccccttcg tcccgccaaa cggcacctcc ggggggatct tcaggtgctg gttgacccag
      601 acgatccccg cctcaagccc ctccgtcgcc cgggcgatca gatccgcatt ccgggtccag
      661 accgacgcac cgaggccgta acggctgtcg tttgcccgta taagcgcctc gtcgaggtcc
      721 gcgacggtcg cgaccgggag caccggacca aagacctcct cggagaagag cggggagtcg
      781 tgcgaaaggc cggcaacaag cgtcggcagg aagaagaacc cccgctcgta cccctctccg
      841 cggggagcct gcccgcccgt gacgatctcc ccgtcccggc gttcacaaac cgcatccacc
      901 tcgcgcacca cccgctcaag ccctgcagga ttgttcaacg gacccatatc gacgccgcgg
      961 tccatgccgt tcccgaccac gatcgactcg acccttgatc tcaggcgccg gataaactcg
     1021 tccgcaatcg agtcgaagac gtagagccgt tttaccgccg tgcaggtctg cccgcagttg
     1081 tagaaccgtc ccctgaccgc cccctcgacc gccatcggta tatcggcatc gtcgcagacg
     1141 atcataggat cgctcccccc gagctccagc gtgacccgtt tcaatgccgg cgcggcatcc
     1201 agggcgacct gcctcccggt cccggcctcg ccggtgaacg agaccttccg gatctccgga
     1261 tttcgcgcaa tctcccggcc gaccgtctcc cccggacccg tgacgacgtt caggacgccc
     1321 ggtgggagcc cggcgccctc gaggatcgac gcaagccttc cgcaggtcag cggggccgtc
     1381 gtcgcgggtt tcaggaccat ggtgtttccc gtcgtgagag ccggcccgat cttccagccc
     1441 ataatgatcg ccggcatgtt ccagggtatg atcgcaccgc atacgccaag agggcgccgg
     1501 agaacgatgg tatgcccgta gcccgggagg tttgcgaact ctccccgctc ccctgcggag
     1561 agagcgtaat aatactcgag tatgtttgca aacccgttga tctcgtcgac cgcctcccgg
     1621 atgggtttcc cctgctcggc cgtcaggaga gccgcaaggt cggtgttcct gcggcgcacc
     1681 tcctctgcgg cgaagaagag gatcttcgcc cgggctctcg ggctcttcgc cgaccagtct
     1741 gcaaacgcct ccccggccgc ctccgcggca gaccggacat cctcctcgcc gccaagcggc
     1801 gcctcctcga gcatatcccc cgtcgcaggg ttacgcaccg caaacacctt cccggaggcc
     1861 gcatcgcgcc actcgccgtt gatcaacagt ttcatacgat gatcctatgg atggggccgg
     1921 ttatatgcgc tctgttgggc gggacatctg ccgatcgtgg ccgccatcgc cgccgccagc
     1981 ccggcaggca ggaggaaccc ggcgaggaac ccgaactccc ctgcgatcgc cccggcaaga
     2041 gcggggagga ccgtaaaccc ggcataggtc gcggtgttga agagcccgat cataacgccc
     2101 tgccggatcc cggtctctgc aaggaacgcg agcaccgcga cgatgatcac cccggcaaga
     2161 gccccgacga ccgggagggc atacggtgtt gcgtaactcg ccgcgaccgc cccggccatg
     2221 aggagcgaag aggcccggat ggccggggcg ggacggagat cgaggcgggg aacgacgagc
     2281 acggcgatga cggtcgcgat gttgaccgtc gcgagctgga gggcaagaag cgacggatcg
     2341 cctccggtgt acccggggta gagcgccgcc accatccccg tcatccctac gaggacggct
     2401 gccgtggtaa agagccagaa gtagttccgg gcgatgagcg agagatcggc ctctacaaac
     2461 ccggcgtggt tgctctccct gatggcggcg ctcatcacga gcgcagggat ggcaagcgcc
     2521 gtgaagaaag cgatcccggc aagatggctg tcgaagacgg catcgagcca ccccgcggcg
     2581 agaagtcccg cgaccaggcc gaggttctgg gctgccatga cgtatccgct gacccggccg
     2641 gccgacgggt gcgagtttgc ccatgcaagc gccgccgaaa ggaagaggcc ggccccgatc
     2701 ccctcgaccg cccggaagag aaggagcggg aggcctgaag ggaagagcaa catgaaaacc
     2761 ccgcttgcaa ccgtcaggaa aaggccggcg cggatgagcg gcacgcggcc gacccgatcg
     2821 cttgccatcc cggccggcag taccatgatg aacgcgccca aaaaatatgc ggcgtagacc
     2881 gcgccctgca tcgctgcccc ctccgcaaaa cccgggagca ccggaacgac ggcgtttgag
     2941 agcgccatca ccacgaatat accggagaga acgggcaggc tccgcggcat cctcagatca
     3001 tgcggtaggt ctcgaggttc aggggagaat gcgtctcgat acggtgccgc ttgtagatag
     3061 aactcgcaag atcgatcgtc ttgttctcgt cgccgtggat ggtgaagatc ttctccgggc
     3121 ggggctggag atgagcgacg tagttcatca gctgtttccg gtccgagtgg ccggagaacc
     3181 cgtcgctcgt cgcgatctcg aggttgatca cgatcgtctc gcgcc
//
LOCUS       DAOSII010000203         3219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_26130_length_3219_cov_49.073641, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000203 DAOSII010000000
VERSION     DAOSII010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26130_length_3219_cov_49.073641"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(267..1343)
                     /locus_tag="PLV90_10205"
     CDS             complement(267..1343)
                     /locus_tag="PLV90_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF97528.1"
                     /translation="MIECVDVAKRFGSTVVLTGVTARIEAGEIFAVIGPSGSGKSTLL
                     RLIDLLDTPTGGTIRIGGTDIHREPERGLAVRRMMGMVFQKPAAFNTTVYENIAVGLR
                     FRGVGEAAIREKVKEVLRMVGLAGYEERRARTLSGGEMQRVALARAIVIEPDILLMDE
                     PTANLDPVSVALIEDLVLRINRDRGTTIVFSTHDMYQGQRLAHRVGVLMKGVFAQVGS
                     PREVFTLPASREVARFVGVENIVDGVVVSENGSSAVDVGGIRIRARSPFGAGEAVALC
                     IRSEDIRIAPADGGAAAPGRNVLSGTVAAIVPRGPFSRVTVDCGFMITSILSWKKVDD
                     LDIREGSQVEVSFAPESVHVVRRE"
     gene            complement(1348..2049)
                     /locus_tag="PLV90_10210"
     CDS             complement(1348..2049)
                     /locus_tag="PLV90_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF97529.1"
                     /translation="MYEIIEGFIEATKLIATFDPDVMAIAARSIIVSFTATIAATLIS
                     VPLGAAISFGSFPGKKSLINLIQTLYSLPTVIVGLLIFLLISRVGPFGFMRLLFTPTA
                     MIIAQTVLILPIMTGLTIAALSGVDPVIRDTLYSLGATRLQFLVNIMKEARFAILMAV
                     AVGFGRAISEVGAAILVGGNIMASSFASSTRTLTTAISLETSMGNIPTSIALGIILLA
                     IALGVNLAITRIQHR"
     gene            complement(2114..3037)
                     /locus_tag="PLV90_10215"
     CDS             complement(2114..3037)
                     /locus_tag="PLV90_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOF97530.1"
                     /translation="MQRRTLFAVAAVMVALLLICGCTGTTTDPTPAEKQQLRIATTTS
                     LDDTKLLNHLSSIFEKQYNAEVLVISAGTGKALEYGQRGDVDVLMVHDRAREDVFLAD
                     GYGINRRVFAYNFFILIGPEADPAGIKGMEPTKAFATIREKGMTDKNVVFVSRGDASG
                     THAKEQALWKGAGFNYSTDIQGSGDWYLEAGKGMGETLTMANEKEAYTLSDIGTYLAY
                     KGNLDLVTLVDEGDQLLNVYCAMQINPAKYPDINSTIAKDWVNFMISDDVQKEIASFG
                     VDKYGQPLFYAAQKDWEKIGVTEAEVTDPIA"
BASE COUNT          606 a         1022 c          981 g          610 t
ORIGIN      
        1 gaccgtcaac tgcacctctc gatcggtctt tgcctccagt gccggcaccc gatcgcgatc
       61 gacccggctc ccgtcgccga tgatcaggac gacgatgtcg ctctgcccat cataggtcct
      121 cgctgcggtg ctccctgtag aatcgcgacc gcgataccct ccggcgcgag ctcttcgatg
      181 ccggcccgga cgagaaagag ggtcatgcag gctcgcttta actcccggac ggcaaaagaa
      241 tcgttatcag gagagaggag ccctcctcac tcccgccgga cgacgtggac cgactccggc
      301 gcgaacgata cctcgacctg gctcccctcc cggatatcca ggtcatccac cttcttccag
      361 gagaggatcg acgtgatcat gaacccgcag tcgaccgtca ccctgctgaa cggcccgcgg
      421 gggacgatgg cggccaccgt gcccgagaga acgttcctcc ccggggcagc ggcgccccca
      481 tccgccgggg cgatccggat atcctcagag cggatgcaga gagcgaccgc ttcccccgcc
      541 ccgaacggcg accgtgcccg gatgcggatg ccgccgacat cgacagcgga agaaccgttc
      601 tcgctgacga cgacgccgtc gacgatgttc tcgacgccca cgaaccgggc gacctcccgg
      661 ctcgccggca gggtgaagac ctcccgcggc gaccccacct gcgcaaacac gcccttcatc
      721 agcacgccga cccggtgagc gagccgctgc ccctggtaca tgtcgtgggt cgagaagacg
      781 atcgtcgtcc cgcgatcgcg gttgatccgg agcaccaggt cctcgatcag ggcgaccgag
      841 accgggtcta agttcgcggt cggctcatcc atcaggagga tgtcgggctc gatcactatc
      901 gcccgggcaa gggccacccg ctgcatctcc ccgcccgaga gcgtccgcgc ccgccgctct
      961 tcgtaaccgg caaggccgac catgcggagg acctccttga ccttctcacg gatagccgct
     1021 tcccccaccc ccctgaaccg gaggccgacg gcgatgttct cgtagaccgt cgtgttgaag
     1081 gcggccggct tctgaaagac catccccatc atccggcgga cggcaagacc cctctccggc
     1141 tccctgtgaa tatcggtgcc gcctatccgg atcgtccctc ccgtgggcgt atcgaggaga
     1201 tcgatgaggc gcagcagggt cgacttcccc gacccggagg ggccgatgac ggcaaagatc
     1261 tctcccgcct cgatccgcgc cgtgacgccg gtgagcacca ccgtgctacc gaaccgcttt
     1321 gcgacgtcga cacattcaat catcgttcta cctgtgctgt atcctggtta tggcgaggtt
     1381 cacgccgagg gcgatggcaa gcagtatgat accgagcgct atcgacgtgg ggatgttgcc
     1441 catcgacgtc tcgagcgata tcgcggtcgt cagggtccgg gtcgaactcg caaacgacga
     1501 cgccatgatg ttgccgccga cgaggatcgc cgccccgacc tcggatatcg cccggccaaa
     1561 tccgaccgcg acggccatga ggatggcaaa ccgtgcctcc ttcatgatat tcacgagaaa
     1621 ctggaggcgc gtcgccccga gcgagtagag ggtatccctg atgaccggat cgacccccga
     1681 gagagccgct atcgtaaggc cggtcatgat cggcaggatg agcaccgtct gggcgatgat
     1741 catcgccgtc ggggtgaaga gcagccgcat gaacccgaac ggcccgacac gggatatgag
     1801 caggaagatg aggaggccga cgatgaccgt cggcagggag tagagcgtct ggatcaggtt
     1861 gatgaggctt tttttccccg gaaacgaacc gaagctgatc gcggcgccga gcgggacgga
     1921 gatcagagtg gcggcgatcg tcgcggtgaa cgagacgatg atgctccgtg cggcgatggc
     1981 catcacgtcg gggtcgaacg tggcgatgag ctttgtcgcc tctatgaagc cctcgatgat
     2041 ctcgtacata ctctctccag tcgtgggtcg tcgaaagcaa aaaaagggtt ggaatcaggg
     2101 gccatctgcc ggatcacgcg atgggatccg tcacttcggc ctcggtcacg ccgatcttct
     2161 cccagtcctt ctgggcggcg tagaagagcg gctggccgta cttgtcgacg ccgaaagatg
     2221 cgatctcctt ctggacatca tcggatatca tgaagttgac ccagtccttc gcaatcgtgc
     2281 tgttgatatc ggggtacttc gcagggttga tctgcatggc gcagtagacg ttgaggagct
     2341 gatcgccctc atccacgagc gtcacgaggt cgaggttgcc cttgtaggca aggtaggtcc
     2401 cgatatcgga gagcgtatag gcctccttct cgtttgccat cgtaagggtc tcgcccatgc
     2461 ccttcccggc ctcgaggtac cagtcgccgg agccctggat atccgtcgag tagttgaacc
     2521 cggctccctt ccagagggcc tgctcctttg cgtgcgtccc cgacgcatcg ccgcgcgaga
     2581 cgaagacgac gttcttgtcg gtcattccct tctcccggat ggtggcaaac gccttcgtgg
     2641 gctccatgcc cttgatgccc gccgggtcgg cctccggccc gatgaggatg aagaagttgt
     2701 aggcaaatac gcgccggttg atgccgtagc cgtcggcgag gaagacatct tcgcgcgcgc
     2761 ggtcgtgcac catcaggacg tccacgtcgc cccgctgccc gtactcgagc gccttgcccg
     2821 ttccggcgga gatgaccagc acttcggcgt tgtactgctt ctcgaagatc gaactgagat
     2881 ggttgaggag cttggtgtcg tcaagactcg tcgtcgtagc gatacggagt tgctgctttt
     2941 ctgcaggcgt tgggtcggtc gtcgtcccgg tacagccgca gatgagcagg agagcgacca
     3001 tgacggccgc aactgcaaaa agggttcttc gctgcatagt tctcaaagag gtgtcgcaat
     3061 acacatattt aaatagggcg gttttgtgtt aatggtaact caatttattt aataaaaaaa
     3121 tgttgaccag aggggccgtc aggcccggat gatggtgccg acccgctccc cgttcacggc
     3181 ttttgttatg ttgcccggca catgggcgtt cactatctt
//
LOCUS       DAOSII010000204         3188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_26433_length_3188_cov_31.332589, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000204 DAOSII010000000
VERSION     DAOSII010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26433_length_3188_cov_31.332589"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLV90_10220"
     CDS             complement(<1..183)
                     /locus_tag="PLV90_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97531.1"
                     /translation="MLFQFFDLLLDRPVGYAYDPGEFGNGHGWVMLHDFDDLLGTFWG
                     TFWGTFWGTFWGTFWGT"
     gene            237..704
                     /locus_tag="PLV90_10225"
     CDS             237..704
                     /locus_tag="PLV90_10225"
                     /inference="COORDINATES: protein motif:HMM:NF016220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HOF97532.1"
                     /translation="MTFTEANTVEQLILDAVTRKRQAARLTVQEAPRAGSPGDIHPAG
                     WTYVPAREIPRRPGDVMVESWLRDALIRLNPRIAAEPGRADEVIYNLRAILLAVQADG
                     LVRANENFMAWLRGEKTMPFGKNGEHVPVRLVDADNPGGNHLVVTNQWTYPAR"
     gene            683..1900
                     /locus_tag="PLV90_10230"
     CDS             683..1900
                     /locus_tag="PLV90_10230"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HOF97533.1"
                     /translation="MDLPGPIEKRFDIVFVVNGLPLVIGEAKSPARSAVTWFDGAYRV
                     SEIYEKQVPAIFVPNVFSFATEGRVYRYGSIRMPIDLWGPWRTEENEPEGTLADVSRS
                     VESMLQPEVVLDLLQYYTLFATDKKHRRIKIIARYQQFVTANQIVDRVVRGYPKKGLI
                     WHFQGSGKSLLMVFAAQKLRLHPGLGNPTVMIVVDRIDLDTQITATFNAADVPNMVSV
                     ATRQELQTLLAQDVRKVLITIIHKFAEADGDLNERSNIIVLVDEAHRTQEGDLGRKMR
                     EALPNAFLFGLTGTPINRADRNTFWAFGADEDEKGYMSRYSFQESIRDRATLPLHFET
                     PEVKLQIDRAAIDEAYQAMYLDKPMKDHNLLQAICRTNRTFGQKKTHGLIVDYIGIFD
                     DVAHALDFDEKAV"
     gene            1904..2146
                     /locus_tag="PLV90_10235"
     CDS             1904..2146
                     /locus_tag="PLV90_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97534.1"
                     /translation="MITNIDDLRRELPIRVQKCLTFFPGVDRTRGGYEGLMEAQQCLP
                     DNEARDGFAAEYSVLGTIWEALSPDPVLGPYENDFR"
     gene            2261..2800
                     /locus_tag="PLV90_10240"
     CDS             2261..2800
                     /locus_tag="PLV90_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97535.1"
                     /translation="MRDDVETLVLDAQVLAGVPGDADPEKKAREIEIKLIARLRRHAA
                     NPAFIALGERLEKVRDRHEQGFLTSLEFLKAILEVAKDVVEAERETDPEEERDRAREA
                     LTELFNEAKNKATYIIVERIVTDIGDIVKKVRFPDWQHTSQGERLVQKELRRTLLKYK
                     LHTDQELFDRAYAYIRQYC"
BASE COUNT          692 a         1053 c          939 g          504 t
ORIGIN      
        1 agttccccaa aaagttcccc aaaaagttcc ccaaaaagtt ccccaaaaag ttccccaaaa
       61 agttcccaga agatcatcga agtcatgcag catgacccaa ccgtgaccat tgccgaactc
      121 gcccggatca taggcgtaac caaccgggcg atcaagaagc agatcgaaaa attgaaacaa
      181 caaggccggc tccgtcgcgt cggccctgcc cggggcggcc gctgggaggt cctctaatga
      241 ccttcaccga agcgaacacc gtcgagcagc tgatcctcga tgccgtcacc cggaagcggc
      301 aggccgcccg cctgaccgtg caggaagccc cccgggccgg atcgcccggc gacatccacc
      361 cggccggctg gacctacgtg cccgctagag agatcccgcg ccggccgggc gacgtgatgg
      421 tcgagtcatg gctgcgggac gcgctcatcc gtctgaaccc ccggatcgcc gccgaacccg
      481 gccgggccga cgaggtcatc tacaacctcc gcgccatcct ccttgccgtg caggccgacg
      541 gcctcgtccg ggcgaacgag aacttcatgg cctggctccg gggcgagaag accatgccct
      601 tcgggaagaa cggcgaacac gtcccggtgc ggctcgtcga tgccgataac ccgggcggta
      661 accaccttgt cgtcaccaac cagtggacct acccggcccg atagagaagc ggttcgatat
      721 cgtcttcgtc gtcaacggcc tcccgctggt catcggcgag gcaaagagcc ccgcccggag
      781 cgctgtgacc tggtttgacg gcgcataccg ggtcagcgag atctacgaga agcaggtgcc
      841 ggcaatcttc gtgccgaacg tcttctcctt cgccaccgag gggagggtct accgctacgg
      901 ctccatcagg atgcccatcg acctctgggg cccgtggcgg acggaggaga acgaaccgga
      961 ggggacgctt gccgacgtga gccggtcggt cgagagcatg cttcagcccg aggttgtgct
     1021 cgacctcctg cagtactaca ccctctttgc caccgacaag aagcaccgcc gcatcaagat
     1081 catcgcccgc taccagcagt tcgtcaccgc caaccagatc gtggaccggg tcgtcagggg
     1141 ctacccgaag aaagggctca tctggcactt ccagggcagc ggcaaatcgc tcctcatggt
     1201 ctttgccgcg cagaaactcc gcctgcaccc gggactcggc aaccccacgg tgatgatcgt
     1261 cgtcgatcgc atcgacctcg acacccagat caccgccacc ttcaacgccg cagacgtccc
     1321 gaacatggtc agcgtcgcca cccggcagga actccagacc ctcctcgcac aggacgtccg
     1381 gaaagtgctc atcaccatca tccacaagtt cgccgaagcc gacggagacc tgaacgaacg
     1441 ctcgaacatc attgttctgg tcgatgaggc gcaccgcacc caggagggag atctcggccg
     1501 taagatgcgc gaggcgctgc cgaacgcgtt cctcttcggc ctcaccggga cgcccatcaa
     1561 ccgggccgac aggaacacgt tctgggcatt cggcgccgat gaagacgaga agggctacat
     1621 gagccgttac tcgttccagg agtccatccg cgaccgggcc accctgccgc tgcacttcga
     1681 gacccccgaa gtgaagttac agatcgaccg ggcggcgatc gacgaggcct accaggcgat
     1741 gtatctcgac aaaccgatga aggaccataa cctcctgcag gccatctgcc ggacgaatcg
     1801 caccttcggc cagaagaaga ctcacggcct catcgtggac tatatcggca tcttcgatga
     1861 cgtggctcat gcgctcgact tcgacgagaa agccgtgtag caggtgatca caaacatcga
     1921 cgacctgcga agggagctcc cgatccgggt gcagaagtgc ctcaccttct tccccggggt
     1981 ggatcggaca aggggcggct acgaagggct catggaagcg cagcaatgtc tgcccgacaa
     2041 cgaggcacgc gatgggtttg cggcggagta ctccgtgctc ggcaccatct gggaggcact
     2101 ctcgccggac cccgtgctcg ggccgtacga gaacgacttc cggtagatca cgcaggtcta
     2161 cgaatccgtc aggcccccaa gcggccacgg gaggctgctc tggcacgccc tcggcgcaaa
     2221 gaccgtcgat ttgatcaaca aaaacgtcca cgtccagtca gtgcgggacg atgtcgagac
     2281 gctggtgctg gacgcgcagg tgctcgccgg ggtcccgggc gacgccgatc cggagaagaa
     2341 agcccgcgag atcgagatca agctcatcgc ccggctgcgc aggcatgcgg caaacccggc
     2401 attcatcgcg ctgggcgaac ggctcgaaaa ggtgagagat cgccacgagc agggtttcct
     2461 caccagcctg gagttcctca aagccatcct cgaggtggca aaagacgtcg tcgaggccga
     2521 acgggagacc gacccggagg aggagcgcga ccgggccagg gaagccctca ccgagctctt
     2581 caatgaggca aagaacaaag ccacctacat cattgtcgag cggatcgtga ccgatatcgg
     2641 cgatatcgtg aagaaagtgc gtttccccga ctggcagcat acctcgcagg gcgagcggct
     2701 ggtccagaag gaactccggc gcactctcct caagtacaaa ctccacaccg accaggaact
     2761 cttcgatcga gcctacgcct acatccggca gtattgctaa tgaccggagc aatcctgtta
     2821 ttctcgatgg tgatcgtcag cgtcgcctcc gctttgttga accaccggca tttgtctcta
     2881 ccgatttacg gcggtatgca taccaccaaa acaggttccc ctccaaaagc cggatcgtgg
     2941 tggctccgga gaacccgggg ccgccgggcc ctctcatcac gggtaccgag atctggggcc
     3001 ggccgcctct gtagacgacg aacgagcggc cccgggcagc agacctcctc acggttgagg
     3061 gcgatcgcct gcagggtttc gcctgcccgt gacgggccgg cataaccggg agatcgtaca
     3121 gttcccggct ggaattcata cccaccccgg gggagccggt aaaagaatat gagctgatat
     3181 aaatgcag
//
LOCUS       DAOSII010000205         3185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_26467_length_3185_cov_28.353355, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000205 DAOSII010000000
VERSION     DAOSII010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26467_length_3185_cov_28.353355"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PLV90_10245"
     CDS             complement(<1..189)
                     /locus_tag="PLV90_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97536.1"
                     /translation="MGAVVGVEPLPGRITSTPAISVVQRSIGGAGALMPLLPPSMPGT
                     LWVVTLIACAARVQDTYAV"
     gene            968..2557
                     /locus_tag="PLV90_10250"
     CDS             968..2557
                     /locus_tag="PLV90_10250"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF97537.1"
                     /translation="MISVLYVDDEPALLELTRLFLERYGNYSVDTAISALDALDMMKA
                     APYDAIISDYQMPVMDGIALLKHLRAEDSSTPFILFTGRGREEIAIEALNAGADFYLQ
                     KGGEPGSLFAELEHKLRLAVERRRIADELSESRQRMTAIVSHLPDATFAIDLEGKVIV
                     WNRAMEEMTGVKEEEILGTGDHACAAAIYRARRPILLDFIIGREEKIKEYYPDLTVKD
                     DRLITENYIPTLYGGRGAHVWLVASPLYDAHGTITGAIESIRDITDRKLAEEALHRSE
                     EKYRTLTEQTNDIIYLMDVNGRLTHIGPQVARYGYTPEEIISQNLSVFVVEEDVAIVV
                     DDLEKAIATGIPRYTVFRARDKSGNIYWFEDSGAPVFDENGVIVGVSGALRDITDRRR
                     IEQKLVESERIYRSILENMRDVFYRSDREGILIMVSPSGAELLGYGSVEELLGKKIDS
                     TLYADRGEQDRFLAEMDKRGEVVNYRVILKRRDGSPIPVETSSRRYYDENGCCLGIEG
                     FFRSAPGPGFRGETSGEYSRC"
     gene            2737..3162
                     /locus_tag="PLV90_10255"
     CDS             2737..3162
                     /locus_tag="PLV90_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97538.1"
                     /translation="MLAVPGIGPGAVPLLQVMAAKQYRCNDTMDEAPSPGHARTKPGR
                     RQPNGCRGAVLAPARFMTALMAGAALVPGYSINTDAIGVLGLVFLAAYAVELYRYREP
                     SWPRRTGETDHLPRALLAHGIRGLPHGAGYLLPVALKKV"
BASE COUNT          662 a          933 c         1027 g          563 t
ORIGIN      
        1 cacggcatac gtatcctgta ctctcgccgc gcatgcaatt aaggtgacga cccatagggt
       61 cccgggcatc gatgggggga gaagaggcat gagcgcaccg gcacccccta tcgatcgctg
      121 caccacggat atcgccggcg tcgacgttat ccgccccggg agaggctcga cgcccaccac
      181 cgcgcccacg cccgggcgtt tgggagccgt tacgagcaga cgacctttgg cgaccggaaa
      241 gcgcacttcg ccggggtccc gcgggtcgac cctgccctca tccggctacg gggatccgcg
      301 tgggctactg cataacctcg atctccccgg agcatgtggg caagatcgag tctctcttta
      361 tcggcgaggc ataccgctcg cgggggaacg gcacgacctt catgaaccgg gcgcctgcgt
      421 gactggacgg gaacggttcg attgagaacc gggcctcggt cgctgacggg aacgaggagg
      481 cgttctcgtc ctaccggcgg ttcgggttct acccccgccg ggcggtgctc gaacagaaga
      541 gggagtagcg tggaggccgc tacatccaga gagagttcat cgccacatgg gagcgagagg
      601 agttccggcg ctgcaggagg gcatcttccc gtggcgtgcc gggcatcacc ggtcaatcgc
      661 gattctggcg gcaacgggga cccaccgctc ctgtgcctcc gggagaaggg agccgggaaa
      721 gtcattcgga gaacaccggc cgggcctgac cggggctccg gccatggtgg tcgcatcggc
      781 cgcatggcgg gaggagacgg ccatcgtatc ttaccggtat atgccagaac ccatcctgct
      841 tcaccacatt ctctcgtaag agcacgtgtg agggagagca ggaagggata agcttatata
      901 tgccactgcg atatgtagac ataaccgact atccatatcg ggccgtcggc aacaccgggg
      961 gaccatcgtg atatcagtcc tttacgtcga tgacgaaccc gctctcctgg aactgaccag
     1021 gctattcctg gagcggtatg ggaactactc agtcgatact gcgatatctg cactcgatgc
     1081 cctcgacatg atgaaggcag ccccctacga tgccatcatc tccgattacc agatgcccgt
     1141 gatggacggc atcgcgctcc tcaaacacct gcgggccgag gacagcagca ccccgttcat
     1201 cctgttcacc ggccgcgggc gtgaggagat cgcgatagag gccctcaacg ccggtgcaga
     1261 cttctatctt cagaaaggcg gcgaaccggg gtcgttgttc gcggaactgg agcataagct
     1321 ccgcctggcg gtcgagcggc ggcggattgc ggatgaactc agcgagtccc ggcagcgaat
     1381 gaccgccatc gtcagccacc tgccggatgc caccttcgcg atcgatcttg aggggaaggt
     1441 catcgtatgg aaccgcgcga tggaagagat gaccggtgta aaagaggagg agatcctggg
     1501 gaccggggac cacgcgtgtg ccgccgcgat ctacagggcg agacggccga tcctcctcga
     1561 cttcatcata gggagggagg agaagatcaa agagtattac cccgacctca ccgtaaaaga
     1621 cgacaggttg atcacggaga actacatccc caccctctac ggaggaagag gtgctcatgt
     1681 atggcttgtc gcatccccgc tctacgacgc gcacggcacc atcaccgggg cgatcgagtc
     1741 catccgcgac atcaccgacc gtaaactggc agaggaggcg ctccacagga gcgaggagaa
     1801 gtaccggacc ctgacggaac agacaaacga catcatctac ctgatggacg tcaacggtcg
     1861 actcacccac atcggccctc aggttgccag gtacgggtat actccggagg agatcatatc
     1921 gcagaacctt tccgtgtttg ttgtcgagga agatgtcgca atcgtggttg atgatctcga
     1981 gaaggccata gcgacgggaa tacccaggta taccgtattc cgcgcgcggg ataaatccgg
     2041 caacatctac tggtttgagg atagcggcgc accggtcttt gatgaaaacg gtgtcattgt
     2101 cggggtatcg ggggccctgc gggatatcac cgaccggaga aggatcgagc agaagctggt
     2161 tgagagcgag aggatctacc gatcgatcct ggagaacatg cgggacgtct tttaccggag
     2221 cgaccgcgaa ggcatcctca tcatggtgag cccctccggg gcagagctcc tgggatacgg
     2281 ttcagtcgag gaactgctcg gaaagaagat cgactcgacg ttgtatgcag accggggaga
     2341 gcaggatagg ttccttgccg agatggataa acggggagag gtcgtcaatt acagggttat
     2401 cttgaagcgg cgtgacggaa gcccgatccc cgtggagacg agcagccgca ggtactacga
     2461 cgagaacggg tgctgcctgg gcatagaggg gttcttcagg agtgcccccg gacccggatt
     2521 cagaggcgaa acctccggcg aatactcacg gtgttgaagt ctccaccgat tgcaccttga
     2581 gagagcctgc tgcaaccggg tgagagccac gcgtgagccc ggggggtcga ccctcgcgaa
     2641 ggacgggtct tcggcgcatc gcatgccggc gggtatcagg tgaaccgccc gctgcctgaa
     2701 gagcgggatc tccgtggata aggagatggg cgggttttgc tcgccgttcc ggggataggg
     2761 ccgggggccg taccgctcct gcaggtgatg gcggcaaagc aataccggtg caacgatact
     2821 atggatgaag cgccgtcgcc gggccatgca cggaccaaac ccggtagacg gcaaccgaac
     2881 ggttgcaggg gtgctgttct tgcccccgcc cggttcatga ctgcgctcat ggcgggcgcg
     2941 gccctggtgc ccggctacag catcaacacg gatgcgatcg gcgttctcgg cctcgtcttc
     3001 ctggcggcgt acgccgttga gctatatcgg tatcgcgagc cttcatggcc ccggcggact
     3061 ggagagaccg atcatctacc ccgcgctctt ctggctcatg gcattcgggg gctccctcat
     3121 ggcgccggct atcttctccc ggtcgccttg aagaaggtat agcagaagac tccgtccggt
     3181 tccgc
//
LOCUS       DAOSII010000206         3174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_26573_length_3174_cov_33.706637, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000206 DAOSII010000000
VERSION     DAOSII010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26573_length_3174_cov_33.706637"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PLV90_10260"
     CDS             <1..191
                     /locus_tag="PLV90_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HOF97539.1"
                     /translation="SYERAMAPGAAFVGLTPPLRDRVLLSSRALAGVEGALRLMDEVL
                     NACTSRNRRVGSDYTDGF"
     gene            275..643
                     /locus_tag="PLV90_10265"
     CDS             275..643
                     /locus_tag="PLV90_10265"
                     /inference="COORDINATES: protein motif:HMM:NF025903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HOF97540.1"
                     /translation="MPLVKIEIRTGKPIEYRQLLIESLRRAFMDALQTPEAALWIRVC
                     ERKEENFPMPPGRSADAVLIEASLMPGRGPRAKRALYEAIVGHLARDAGIRPDDVCIV
                     LYEPPIENWAEHGEAFAPSR"
     gene            746..955
                     /locus_tag="PLV90_10270"
     CDS             746..955
                     /locus_tag="PLV90_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97541.1"
                     /translation="MTHYGGRAVHGMLLERMFDILDEDQVQQLMIRMIESSIKAKQQH
                     IDMMQYKIETYKMARDMLQAEMKNK"
     gene            1039..1503
                     /locus_tag="PLV90_10275"
     CDS             1039..1503
                     /locus_tag="PLV90_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF97542.1"
                     /translation="MDLIIREYREEDFLSVSRLERENSPGDCKPEIFIRQAGVLFAGT
                     FLVAEVEGEVVGYTIGALVQHRQTTGWIVRLVVSGEHRRRGFGERLITAVAGSLRERG
                     AEEVYLSVAPSNHAARALYEKHGFREADFCPAYFGEGADRSILRRDLTAGKA"
     gene            1529..2113
                     /locus_tag="PLV90_10280"
     CDS             1529..2113
                     /locus_tag="PLV90_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOF97543.1"
                     /translation="MSFTPIRIGEVFRPTPAFRPYLYASLVLAVVFLVLPWLVPIILL
                     AWGIVAFAFALPTLAAIVFVIYWIPRYCESIVYRLTVTEITWQRGVWFQQTGIVPYNR
                     ITNVDIAQGPLMRLFAFSAVRVQTAGYSAQAQAEIVLNGIADPKDLQERIMDFVRRTG
                     PVAVEGGPEEVPAAGAIVEELRAIRRLLESREGR"
     gene            complement(2212..2685)
                     /locus_tag="PLV90_10285"
     CDS             complement(2212..2685)
                     /locus_tag="PLV90_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOF97544.1"
                     /translation="MFKRILFPTDFSAPSMKVLDYIPALQEVGTREVVLVHVIDSKDI
                     TLIASGGQGFLGTVSDRETETQRRLREEIEHQIIDARRSLEKRGLKVTVRTPVGNPEK
                     EILSAADAEEASLIVIGSHGRSNIRDRLLGSVSEYVIKNARQPVLVIKRDTAGAK"
BASE COUNT          576 a          970 c         1011 g          617 t
ORIGIN      
        1 cctcctacga gcgggcgatg gctccgggtg ccgcgttcgt cggcctgacg ccgcctctca
       61 gggatagggt tctcctctca tcccgcgcgc tggccggggt cgagggggcg ctccggctga
      121 tggacgaggt gctcaacgcc tgcaccagcc gcaaccggcg ggtggggagc gattacacgg
      181 acggcttcta aggaaccgtt aatacccggg gtgccagagc actcatgccg gagaacggga
      241 atctctcccg gatacccggc tggagctggc ccccatgccc cttgtaaaga tcgagatccg
      301 caccggaaaa cccatcgaat accggcaatt gctcatcgaa agccttcgca gggctttcat
      361 ggatgcactg cagacaccgg aggccgccct ctggatccgg gtctgcgagc gaaaagagga
      421 gaactttccc atgcctcccg gccgatccgc cgatgcggtc ctgatcgagg cctccctcat
      481 gccggggcgt ggtccccgtg cgaagagggc gctctatgag gcgatcgtcg ggcaccttgc
      541 ccgcgatgcg ggcatcaggc cggacgatgt ctgcatcgtc ctctacgaac cgcccatcga
      601 gaactgggcc gagcacggtg aagcgttcgc cccatcccgg tgaatccgcc ggggtacctg
      661 cacccggcac aaggtattta tcacccgccg ggcacagggt gccggcctgt ccgtatgggc
      721 agggccgaca caaggggggt atgatatgac gcactatggc gggcgcgctg ttcacggtat
      781 gctccttgag aggatgttcg atatcctcga cgaggatcag gtgcagcagt tgatgatccg
      841 gatgatcgag tccagtatca aggcaaaaca gcagcatatc gatatgatgc agtacaaaat
      901 cgagacgtac aagatggcgc gcgatatgct ccaggcagag atgaaaaata agtgagccct
      961 ttgccccggg ggcgctccgg cgatcgagcg gcccggggct cccttctctt tttctccctt
     1021 cgcgccgaac ataccggtat ggacctgatc atcagggagt atcgcgaaga ggattttctc
     1081 tcggtctctc gcctcgagcg ggagaacagc ccgggtgact gtaagcccga gatcttcatc
     1141 cggcaggcgg gcgttctctt cgccgggact ttcctcgtcg cggaggtcga gggagaggtc
     1201 gtcggctaca ccatcggggc cctcgtgcag caccggcaaa cgacgggatg gatcgtccgg
     1261 ctggtggtct cgggggagca ccgccgccgg gggtttggag agcggctcat caccgccgtc
     1321 gccgggagcc tccgggagag gggggcggag gaggtctacc tctcggttgc accgtcaaac
     1381 catgccgccc gggcgctcta cgagaagcat ggttttagag aggcggattt ctgccccgca
     1441 tacttcggtg agggggccga tcgctccatc ctccggagag acctcacggc agggaaggct
     1501 taatccttcc aggttgatat gtgatttcat gtcgttcacc ccgatccgta tcggcgaggt
     1561 cttcaggccg acaccggcgt tccggccgta cctctacgca tcgctggtcc tcgccgtcgt
     1621 ctttctcgtc ctcccctggc tggtccccat catcctgctg gcgtggggca tcgtcgcttt
     1681 cgccttcgct ctgcccaccc ttgccgccat cgtattcgtc atctactgga taccccgcta
     1741 ctgcgagagc atcgtctacc ggctcaccgt caccgagatc acctggcagc ggggagtctg
     1801 gttccagcag acgggtatcg tcccctacaa ccggatcacg aacgtcgata tcgcccaggg
     1861 ccccctgatg cggctctttg ccttctcggc agtccgggtg cagaccgccg gctactcggc
     1921 tcaggctcag gcggagatcg tcctcaacgg gatagcggat ccaaaggatc tccaggagag
     1981 gatcatggac ttcgtccgga ggacgggccc ggtcgccgtc gagggcgggc cggaagaggt
     2041 gccggccgca ggtgcgatcg tggaggagct gcgggcgatc cggaggctgc tcgagagccg
     2101 tgaggggcgg taggagaggt gccggccggg gaatatggat gtgggtgcgg gctcacctgc
     2161 gggcggtgcg gcccgcccga tgacgtgagc gggccaggag aaagaataac cttatttcgc
     2221 gccggccgta tcccgcttga tgaccaggac cggctggcgg gcgttcttga taacgtactc
     2281 cgagaccgac ccgagcaggc ggtcgcggat gttcgaccgg ccgtgggaac cgatgacgat
     2341 gagcgaggcc tcttccgcat ccgccgcgga caggatctct ttctcagggt tgccgacggg
     2401 tgtccggacg gtcactttca ggccccgttt ctcaagcgat cgccgggcat cgatgatctg
     2461 gtgttcgatc tcctcccgga gccggcgctg ggtctcggtc tcacggtctg atacggtccc
     2521 gaggaacccc tgcccgcctg aagcgatcag cgtgatatct tttgagtcga tgacgtggac
     2581 gagaaccacc tcccgggttc ccacctcctg cagcgcaggg atgtagtcca gcaccttcat
     2641 ggagggcgct gaaaagtccg ttggaaacag gatgcgtttg aacatgctct gacactctct
     2701 gatatttctc tctgtgggct ggatatttaa tggcaacgcc tgttgcagcg tgctgaaagc
     2761 aggcgcaaag aggggacttg cgctgcaagg ggttcctccg ggcggcctgg ttcgccggat
     2821 atttgagtgc gtgaagtgac gctctgcaag cacgcatacc acgagtgttt ctcacgcagg
     2881 catagccgga tgaggcggtt gcacggatag tgtggttcgt gcgtcacatt gcccgacaag
     2941 aaaggcgcga ccattttgtt gacgtcaaca aaatggtctg gattgtttca aaccccacgt
     3001 ccgtgtagaa tgtgaccctc cctcattagg aggtatctgc acgacagagt ttcaattcat
     3061 caacccttac ccggaagaac ggccgagaat cagcaacccc ctgtgttcga ccgccccctc
     3121 ttcacccccg gatctgcacc cgccaccggt tgagcatccg gcggacaccg gcgg
//
LOCUS       DAOSII010000207         3129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_27042_length_3129_cov_45.055303, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000207 DAOSII010000000
VERSION     DAOSII010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27042_length_3129_cov_45.055303"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PLV90_10290"
     CDS             <1..265
                     /locus_tag="PLV90_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97545.1"
                     /translation="AVAYVPREVFRAVPRAHQIILTERVKHLPTRFWYMERTLAHGWS
                     RNVLPQKRLFMDTLYRSPGNVSSLSCIGLRRPVYMRDPWGAMQ"
     gene            262..495
                     /locus_tag="PLV90_10295"
     CDS             262..495
                     /locus_tag="PLV90_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOF97546.1"
                     /translation="MRNLNYRILLRKEPEGGYTVTVPTLPGCVTYGETVDEAIAMARE
                     AIELYIEHLQEKGEEIPTEEGLLEYTLTVEAHA"
     gene            858..1100
                     /locus_tag="PLV90_10300"
     CDS             858..1100
                     /locus_tag="PLV90_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin family protein"
                     /protein_id="HOF97547.1"
                     /translation="MTKTLKVLFDGTVFRPSGPVDLEAGKKYTITIQLSPENSDATAD
                     PAFDIASLAVDTHVSDLAAEHDHYLYGTPKRDTHGQ"
     gene            1090..1515
                     /locus_tag="PLV90_10305"
     CDS             1090..1515
                     /locus_tag="PLV90_10305"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOF97548.1"
                     /translation="MGNDRCFVDTGCWIALLNKRDNLHKTADTIYKRRMESGSSLVTT
                     SSVLTETFNALANPIFKPSVIAFRRRLESSPRVEIVFVDPALWSRGWNIYEQRLDKAW
                     SLTDCISLAVMKEQEISDALTADHHFVQAYHALLAEGSG"
     gene            1918..2541
                     /locus_tag="PLV90_10310"
     CDS             1918..2541
                     /locus_tag="PLV90_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97549.1"
                     /translation="MLRLEDITKDAAVNGIEPGQIVRIVTTEPIGENALTVYYRTADG
                     RVKEQMLFRSSEASLSLAEAGRPWAFDAPGRMKGGRDLSWIAGCASTEALLHPGWSVA
                     TDLPPLPEADELPEGRSREGRGQPVGHPSPPEDEPVVFPGDLAHGDAEVVGPSCGRRP
                     TSRVLRGGRGYLCYDYFKTAKRPAKGGSRALHRASPGKRWGDSPPGI"
     gene            complement(2572..2790)
                     /locus_tag="PLV90_10315"
     CDS             complement(2572..2790)
                     /locus_tag="PLV90_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOF97550.1"
                     /translation="MYRFLVIVEQTDGNYSAYSPDLPGCVATGATREEAEDRMHEAIE
                     LHIEGLREDGLPIPPSRTSAIYLAVGKG"
     gene            complement(2794..2988)
                     /locus_tag="PLV90_10320"
     CDS             complement(2794..2988)
                     /locus_tag="PLV90_10320"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOF97551.1"
                     /translation="MKVKDCIKMIEADGWYLVATRGSHRQYKHPSKSGRITLAGHPAD
                     LLAPGTLEQYPQTGRAEREE"
BASE COUNT          611 a          928 c          967 g          623 t
ORIGIN      
        1 agcggtggcg tacgttccgc gagaggtctt ccgggcggtt ccccgggctc atcagatcat
       61 cctgacggag agggtcaagc acctcccgac ccggttctgg tatatggagc ggaccctcgc
      121 gcacggctgg agcaggaacg ttcttcccca aaagaggttg tttatggata ctctttatag
      181 atcgccgggt aatgtatcgt cattatcatg cataggccta cgcaggcctg tatacatgcg
      241 tgatccgtgg ggtgcgatgc aatgagaaac ctgaactacc gtatcctcct ccggaaggag
      301 ccggaaggcg ggtataccgt caccgtaccg acccttccgg gatgcgtcac ctatggggag
      361 actgtcgacg aggcgatagc gatggcacgg gaggctatcg agctctacat tgaacatctc
      421 caggagaagg gcgaggagat tcccaccgag gaaggactcc tggaatacac cctcaccgtc
      481 gaggcacatg cctgaacttc cggcgctcac ggcgcagaag gtcatgaaaa ccggggatct
      541 cttcgagaac tccgaagatc tgaacacacc aggtagcgga agagtggttc cgcgccgtcc
      601 ggcgggggct catccgcgaa ctccggcggg aggctgcgta tatgggcggg ggcgacccgt
      661 cctgctctcc ggggagatgc tcgacctttg aacccggcga ggatcatggc gtaggcgccg
      721 catatccctt gcggaggggg ctttctcgct cataccgccg tcgagccctg caggggcgat
      781 ccggtgcagg cgcacccgca atcattatta tttcacgaag accatgatgg tcatcgagtt
      841 caggggtgga tttcaggatg acaaagacgc tgaaagttct ctttgatgga acggtcttcc
      901 gaccctccgg gcctgtcgac ctcgaggcag ggaagaagta tactattacc atacaactct
      961 ccccggagaa ctccgacgca accgccgatc cggcctttga tatagcatcg cttgccgtag
     1021 acacccatgt cagcgacctc gcagcggagc acgatcacta cctatatgga acgccgaagc
     1081 gggataccca tgggcaatga tcggtgtttc gttgatacgg gatgctggat tgcgttgctg
     1141 aacaagcgtg acaatctcca caaaaccgcc gatacgatct acaaacgccg gatggaatct
     1201 ggatcctctc tcgtcaccac ctcatccgtg ctcaccgaga cgttcaacgc tctcgccaac
     1261 cccatattca agccgtccgt catcgcgttc cgtcgccgcc ttgagtcgtc gccccgggtg
     1321 gagatcgtct ttgtcgatcc cgccctgtgg tcgaggggat ggaatatcta cgagcagagg
     1381 ctcgataaag cgtggagcct cacggattgc atctctcttg cggtcatgaa ggaacaggag
     1441 atctccgatg ccctgaccgc agaccaccat tttgtccagg cctatcatgc cctgctcgct
     1501 gaaggctccg ggtgatcgca ccgggccggg cgcgccgccc gatgaagggg ggatcttcgg
     1561 agatccaggg gtctgttcgg gagctccgaa catttccgcc ccgggttgcc ataggattat
     1621 gcctgaggca tggtcaccga tcttgaagta gggcagagga cgcttgccgt ggggcgtgcg
     1681 acgatcctca tcctcaaaag accgttcccg aaacttcaag cggagcagcc cgcatatacc
     1741 aagacacctc aagaccgtgg gcgagagatt tcggagcatt aatcccggcg cggatcgaga
     1801 ggcaatttca ggcaacgcta tcttctgcag gcggatggag tggtccggca gctcacctga
     1861 ggcggcgagc gttccggccc ggtctccagg ggaaccgtac gaccggaggg ttcagccatg
     1921 ctgcgactgg aggatatcac gaaggatgcg gccgtcaacg gtatcgagcc cggccagatc
     1981 gtacgtatcg tcacgaccga acctatcggc gagaacgccc tgacggtcta ctacagaacc
     2041 gccgacgggc gggtgaaaga gcagatgctc tttcgctcaa gcgaggcctc cctctccctc
     2101 gcagaggccg ggcggccgtg ggcgttcgac gcgcccggcc ggatgaaggg tgggcgggac
     2161 ctctcctgga tcgccgggtg cgcctctacc gaagcgctgc tgcacccggg gtggtcggtg
     2221 gcgaccgatc ttcccccgct gccggaggct gatgagctcc cggaggggcg gtcccgtgag
     2281 gggagagggc agccggtcgg ccaccccagc ccgcccgagg atgagccggt agtcttcccg
     2341 ggggatctcg cgcatggcga tgcggaggtg gtcggacctt catgcggccg gcggccgacg
     2401 tcgcgggtct tgcgcggcgg gagaggatat ctctgttacg actatttcaa gacggcgaag
     2461 cggccggcga agggaggctc tcgagcgcta catcgagcgt ctccaggaaa aaggtggggg
     2521 gattccccac ccgggatctg aaaagcgaac gagtctgatg atcgaggggg ctcagccttt
     2581 accgaccgcc aggtagatgg ctgaagtgcg tgacggcggg atcgggagtc catcctcccg
     2641 gagcccctcg atatggagtt caatcgcttc atgcatccgg tcttccgctt cttcgcgggt
     2701 cgctccggtc gccacgcacc ccggcagatc cggggagtag gccgagtagt tgccgtcggt
     2761 ctgctcgact atcacaagga atcgatacat tattcactcc tctctttcag ccctgcctgt
     2821 ttgaggatac tgttcaagcg ttcccggagc aagaagatct gccgggtgcc cggcgagagt
     2881 gatcctgccg gactttgatg gatgcttgta ctgccggtgg ctgccccggg ttgccacaag
     2941 ataccatccg tccgcttcga tcatctttat gcagtctttc actttcattc tttccctgcc
     3001 gctttaccgt gtgggcgatc gaaacctccg ggggcccggg tggccatcgt gtcacgcacc
     3061 ctgtatgaaa cagtgtatga tggtgctcat atcccttgcg ttatcctctg cgctgcatgc
     3121 ctcatcgat
//
LOCUS       DAOSII010000208         2994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_28675_length_2994_cov_50.606669, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000208 DAOSII010000000
VERSION     DAOSII010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28675_length_2994_cov_50.606669"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1614
                     /locus_tag="PLV90_10325"
     CDS             <1..1614
                     /locus_tag="PLV90_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOF97552.1"
                     /translation="IDEVNLLDDHVADVLLDVAAMGVNVVEREGISFSHPARFILIGT
                     MNPEEGELRPQLLDRFGLQVTVEGIEDPAQRVAIVRAAEVFERDPGGCRQTYGAAQET
                     LRREIVAARMLLPSVSVDDTLLDAVAKACIRLGVRTHRADIAVVRTAKTIAALDGRTV
                     VTRDDVREAMELALPHRMRRKPFEEPRIDPEELERALENAGEGHDRAPRDNGDGAPGN
                     GPSGGHDGDPPPSRGNAAHETVLPVGDPVDPNKVSMPQRRDARERQRASGRRFETFSV
                     KNTGKYVSARYPAGTGDIALDATLRAAAPRQAVRDREGVAIAVRREDIRERVRVGKVS
                     VACVFVVDASGSMGAARRMESAKGAVLSMLLSSYRHRDRIGLVAFRGSSAETLLPLST
                     SIDLAQKQLKELPTGGKTPLAAGLMKGMEVLLQERQKNGEVIPMMVVVSDGRANVSLS
                     GDVCQEVLDIAEEIRLQGIRTVVIDTEETGNSFLEFRLGYCRKIAEHAGGGYYPIADL
                     SAGLLHDVAAAERDAMVHPARKPLTGRAA"
     gene            complement(1595..2935)
                     /gene="purB"
                     /locus_tag="PLV90_10330"
     CDS             complement(1595..2935)
                     /gene="purB"
                     /locus_tag="PLV90_10330"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOF97553.1"
                     /translation="MAIHPIDYRYGTPEMRAVWGEENRFRAIVMAEVALARAEAVHGM
                     IPREDAETIAACALEARLERAKEIEAEINHDMMAVVKAVAEVCGDAGRWIHYGATSND
                     ILDTATALQLRDSLALIEEKLGKLLCVLLARSAETKTLVCAGRTHGQIGVPTTYGLRF
                     AIWASEVARHIERLRQMRPRVVVGHLTGAVGTQAALGDAGIDIQATMMEFLGIRSVDV
                     SNQIIARDRYAEYFMFLANVATTLDKIGLEIRLMQRSEIGELAEAFGKKQVGSSTMPH
                     KRNPIKSEQVCGLARIVRSAVEPALQNNVLWDERDLTNSSPERVLFPEASVLADHILN
                     VMIGVMDGLEFNKKNIRKNLMLLRGVNLAESVMIELTKRGMNRQEAHEAMRTASMQAL
                     AEDRDLSEVLGERPEITTFVTREELDRLLDPDTYIGTAVRQVERVIEKLTPPCR"
BASE COUNT          500 a          964 c         1008 g          522 t
ORIGIN      
        1 atcgacgagg tcaacctgct cgacgaccac gtggccgacg tcctcctcga tgtcgccgcc
       61 atgggcgtca acgtcgtgga gcgggagggg atatcgttct cccaccctgc ccggttcatc
      121 ctgatcggca ccatgaaccc ggaggagggg gaactccggc cgcagttgct cgaccggttc
      181 gggttgcagg tgaccgtgga ggggatcgaa gatcccgcgc agagagtggc gatcgtcagg
      241 gccgccgagg tattcgagcg cgatcccgga ggatgccggc agacgtacgg cgcggcgcag
      301 gagacgttgc gcagagagat cgttgccgca cggatgctgc tcccctccgt ctcggtcgac
      361 gacaccctcc tggatgcggt ggcgaaggcg tgcatccggc tcggagtccg gactcaccgc
      421 gccgatatcg ccgtcgtccg cacggcaaag acgatcgccg cccttgatgg gcggacggtc
      481 gtcacccgcg acgacgtccg ggaagccatg gagcttgcac tcccgcaccg gatgcgccgt
      541 aaaccgttcg aagagccgag gatagacccg gaggagcttg agagagccct ggagaacgcc
      601 ggggaggggc acgatcgcgc tccccgggac aatggagatg gggcgcccgg caacgggccc
      661 tccggcgggc atgacgggga tccgccccct tcccgcggga acgccgcgca cgagacagtt
      721 ctcccggtcg gggacccggt cgatcccaac aaggtgagca tgccgcagag gcgggatgcc
      781 cgggagcggc aacgggcctc cggcaggcga tttgagacgt tctcggtgaa aaatacgggg
      841 aagtacgtct ccgcacgtta ccccgccggc accggcgata tcgcgctcga cgccacgctt
      901 cgggccgccg ccccgcgcca ggcggtgcgc gaccgggaag gggtggcgat cgccgtccgg
      961 agagaagata tccgcgagcg ggtccgtgtg gggaaggtct ccgtcgcgtg cgtcttcgtg
     1021 gtcgacgcca gcggctcgat gggagccgca aggcgaatgg agtcggccaa gggtgcggtc
     1081 ctctcgatgc tgctctcctc ctaccggcac cgcgaccgca tcggtctcgt ggcgttccgg
     1141 gggagcagcg ccgagaccct cctcccgctc tcgacaagca tcgatctcgc gcagaaacag
     1201 ttgaaggagc ttcccaccgg gggaaagacg ccgcttgccg ccggcctgat gaagggcatg
     1261 gaggttctcc tgcaggagcg gcaaaagaac ggcgaggtga tcccgatgat ggtcgtcgtc
     1321 tccgacggtc gggcgaacgt ctcgctctcg ggcgacgtgt gccaggaggt tctcgatatt
     1381 gcagaggaga tccggttgca gggcatccgc acggtggtca tcgataccga ggagaccgga
     1441 aactccttcc tggagttccg gctcggttat tgccggaaga tcgcggagca cgccggtggc
     1501 ggctactacc caatcgccga cctctctgcc gggctcctgc acgatgttgc tgcggccgag
     1561 cgggatgcga tggtgcaccc cgcccgaaaa ccgctcaccg gcagggcggc gtaagtttct
     1621 cgatcacccg ctccacctgc cggacggccg tcccgatgta agtgtccggg tcgaggagcc
     1681 ggtcgagttc ctcgcgggtg acgaacgtcg tgatctccgg ccgctcgccg agcacctccg
     1741 agaggtcgcg gtcttcggcg agtgcctgca tgcttgccgt ccgcatcgcc tcgtgagcct
     1801 cctgccggtt catgccccgc ttcgtcagtt cgatcatcac cgactcggcg aggttcaccc
     1861 cgcgaaggag catcaggttc tttcggatgt tcttcttgtt gaactcaagg ccgtccatca
     1921 cgccgatcat cacgttgagt atgtgatcgg cgagcaccga ggcttcgggg aagagcaccc
     1981 gctcggggga tgagttcgtg aggtcgcgct cgtcccagag gacgttgttc tgcagggccg
     2041 gctcgaccgc agaccggacg atccgtgcaa gcccgcagac ctgctcgctc ttgatcgggt
     2101 ttcgcttgtg gggcatcgtg ctcgagccca cctgcttctt cccgaacgcc tccgcaagtt
     2161 ccccgatctc tgaacgctgc atcagccgga tctcaagacc gatcttgtcg agcgtcgtcg
     2221 cgacgtttgc aaggaacatg aagtactccg cgtagcggtc ccgcgcgatg atctggtttg
     2281 agacatccac ggaccggata ccgaggaact ccatcatcgt cgcctggata tcgatgccgg
     2341 catccccgag ggccgcctgg gttcccacgg cgccggtgag gtgcccgacg acgacgcggg
     2401 ggcgcatctg gcggagccgc tcgatgtggc gggcgacctc gcttgcccag atggcaaacc
     2461 ggaggccgta ggtggtggga acaccgatct gcccgtgggt gcggccggcg cagacgaggg
     2521 tctttgtctc ggcgctccgg gcaagaagca cgcagaggag tttacccagc ttctcctcga
     2581 tgagcgcaag gctatcgcgg agctgcaggg cggtcgccgt atccagtatg tcgtttgatg
     2641 tcgccccgta gtggatccat ctgccggcgt cgccgcagac ctcggcgacg gccttgacga
     2701 ccgccatcat gtcgtggttg atctcggcct cgatctcttt tgcccgctca agacgagcct
     2761 cgagcgcaca tgccgcgatc gtctccgcat cctcgcgggg gatcatcccg tgcaccgcct
     2821 cggcccgggc aagcgccact tcggccatga cgatcgcccg gaacctgttc tcctcgcccc
     2881 agacggcgcg catctccggt gtgccgtacc ggtagtcgat gggatggatt gccatagtgc
     2941 aatacgattg gtgttcccgt gtaaaaaaga ggtcgtgaga gaagggttcc cggc
//
LOCUS       DAOSII010000209         2979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_28841_length_2979_cov_40.396375, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000209 DAOSII010000000
VERSION     DAOSII010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28841_length_2979_cov_40.396375"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PLV90_10335"
     CDS             <1..471
                     /locus_tag="PLV90_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOF97554.1"
                     /translation="GRYGEAVKSIDKIIVLQAGNPAALFMRGAVLEKAGRYDDALASY
                     EKALSVDTENAAIWNATGTLMDTLGRHADAVTAFEAAIELGEGDTHAWLCKGIALSHL
                     GKYDPAAACFDKVLEANPRHARAWYLKGRALDRQGRFAEAVECFKKAIENGGAL"
     gene            471..968
                     /locus_tag="PLV90_10340"
     CDS             471..968
                     /locus_tag="PLV90_10340"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOF97555.1"
                     /translation="MRGYVLAGVLLVLAAASGCVDGGQATVKTGDAVQVHYTGTFANG
                     TVFDSSSGREPLGFTVGSGRMIPGFDAGVVGMREGETKTIHIPADQAYGPHREDLVFA
                     VDPADIPGGENLTAGDPVGVALANGQRLQGRVTDVSPGAVTIDANHHLAGEDLTFTIT
                     LVRIG"
     gene            complement(955..2289)
                     /locus_tag="PLV90_10345"
     CDS             complement(955..2289)
                     /locus_tag="PLV90_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOF97556.1"
                     /translation="MPLATDILIVGLLILANSFFSLAEFALISAKAARLQKRAAGGDA
                     GAAIALELVRDPTQFLSTIQIGITIAGILVGAFGGATLAGPLAGVLAGIPFIAPYSSP
                     IAVATVIIIVTYLTLVIGELVPKRVAMSNAERSASLVARPMQLLTLIATPLVRVLAAS
                     TEAVLRALGVRQPPGPGVTEEDIRVLIGQATRAGIFQEEEQDMVESVFRLGDRRVSVL
                     MTPRPDVVAVDVEDPIEENWQKMAGSRHIYFPVYREHLDNILGIVSIRNLWTRMIAGE
                     SPDLAKAIEPALFIPESARALSILDEFKSSGARVALVTDEYGSIEGLVTVHDIMEAIV
                     GDIPPAGHLPEGPAVRREDGSWLLDGMLPIDEFHDLINVATLPEEERGYYQTLGGFVM
                     MYLERTPRAGDRFTWNGLRFEVVDMDGHRVDKVLVTPGVTEEEEEGVNATRS"
     gene            2457..2930
                     /locus_tag="PLV90_10350"
     CDS             2457..2930
                     /locus_tag="PLV90_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOF97557.1"
                     /translation="MIDVGEQAPEFSIQDQNGNSVRLSDFVGKRVILSFHPLAWTSVC
                     AKQMRALEANGEAFDALNVVALGISVDSVPCKRAWAESLGIKKTRLLADFWPHGGVAQ
                     AYGVFDEEKGYSGRANIVVDESRRVVFAKAYPAATVPDMQDVIDALRAQEQVPQV"
BASE COUNT          555 a          915 c          977 g          532 t
ORIGIN      
        1 gggcggtacg gagaggcggt caagtcgatc gacaagatca tcgtcctcca ggccgggaac
       61 ccggccgccc tcttcatgag gggtgcggtg ctcgagaagg ccggcaggta cgacgacgcc
      121 ctcgcaagct acgagaaggc gctctcggtc gacacggaga acgccgccat ctggaacgcg
      181 accggaacgc tcatggatac actcggccgg cacgccgatg ccgtcaccgc gtttgaggcg
      241 gcgatcgagc tgggtgaagg ggatactcac gcctggctct gcaaggggat cgccctcagc
      301 catctgggca aatacgatcc ggcagccgcg tgctttgata aggttctcga ggcaaatcct
      361 cgccacgccc gggcatggta cctgaaaggg agagcgctcg atcggcaggg aagatttgca
      421 gaagcggtgg aatgcttcaa aaaggcgatc gagaacgggg gagctctttg atgcggggat
      481 acgtgctcgc gggcgttctt ctggtcctcg ccgccgcctc aggctgcgtt gacggcgggc
      541 aggcgacggt gaagacggga gatgccgtac aggtccacta caccggaacc tttgcgaacg
      601 ggacggtctt tgacagttcc agcgggcggg aaccgctcgg gttcaccgtc ggcagcggca
      661 ggatgattcc cgggttcgat gcaggcgtcg tggggatgcg ggaaggagag acgaagacca
      721 ttcacatccc ggccgaccag gcgtacgggc cgcaccggga ggatctcgtc tttgccgtcg
      781 acccggccga tatccccggc ggggagaacc tgaccgccgg agacccggtc ggggtagccc
      841 tcgcgaacgg gcagaggctg cagggaaggg tcacggacgt atcgcccggt gcggtcacca
      901 tcgatgcgaa ccatcacctt gcgggcgaag accttacgtt caccatcacc ctcgtcagga
      961 tcgggtagca ttcacccctt cttcctcttc ttcggtcacg cccggcgtga cgagcacttt
     1021 atcgacccgg tggccgtcca tatccacgac ctcgaaccgg agaccgttcc aggtgaaccg
     1081 gtctccggcc cttggcgtcc gctcaaggta catcatgacg aacccgccaa gcgtctggta
     1141 atacccgcgc tcctcctcgg gcagggtagc cacgttgatg aggtcgtgga actcatcgat
     1201 cgggagcatc ccatcgagga gccatgaccc gtcttcacgg cgaaccgccg ggccctcggg
     1261 caggtgcccg gccggcggga tgtcgccgac gatcgcctcc atgatgtcgt gcaccgtaac
     1321 aagcccctcg atgcttccgt actcgtcggt gacaagggcg acccgcgccc cggacgactt
     1381 gaactcatca aggatcgaca gcgcccgggc gctctcgggg atgaagagcg cgggctcgat
     1441 cgccttcgcg agatcggggg attcacccgc aatcatccgg gtccagaggt tccggatgga
     1501 gacaatgccg aggatgttgt ccaggtgctc gcggtagacc gggaagtata tgtgtcgtga
     1561 tccggccatc ttctgccagt tctcctcgat gggatcctcc acatccacgg ccacgacgtc
     1621 gggccgcggg gtcatcagca cgctgacccg ccggtcgccg aggcgaaaga cgctctccac
     1681 catatcttgc tcctcttcct ggaatatgcc ggcccgggtg gcctgtccga tgagaacccg
     1741 gatatcctct tcagtaaccc ccggacccgg aggctgccgg acaccgagcg ccctgagcac
     1801 cgcctctgtt gaggcggcaa gcacccggac aagcggtgtc gcgatcaggg tgaggagctg
     1861 catcgggcgg gcaacgagcg atgcggatcg ttcggcgttg ctcatagcca cccgtttcgg
     1921 caccagttcg ccgatcacga gcgtcaggta ggtgacgatg atgatgacgg ttgcgaccgc
     1981 gatcgggctg ctgtacgggg caatgaatgg gataccggcg agcaccccgg caagaggccc
     2041 cgcgagtgtc gccccgccga aggccccgac caggatcccg gcgatggtga tgccgatctg
     2101 gatggtggag aggaactgtg tcgggtccct gacgagttcg agggcgatcg ccgcgccggc
     2161 gtcgcctcct gcggctctct tctggaggcg tgccgccttt gccgatatga gtgcgaactc
     2221 cgccagagag aagaagctgt ttgcaagtat caggaggccg acgatcagga tatcggtggc
     2281 aagcggcatt gcgatactct cgctggcaga gatcccgacc gggatctacc ctgcgccggg
     2341 gacggctata cctcaaactc acttatgtgt atcgacggtc cccggctgca gcccggtggg
     2401 atccctcccg aaacacttat cgttaccaga accggagtct cacccggtgg tccgcgatga
     2461 tcgatgtcgg tgaacaggct ccggagttct caatacagga ccagaatggg aacagcgtcc
     2521 ggttgtcaga ttttgtgggg aagcgggtta tcctctcgtt tcacccgctg gcctggacca
     2581 gcgtctgtgc aaaacagatg cgggcgctgg aggccaacgg ggaggcgttc gatgctctca
     2641 acgtcgttgc tctcggcatc agcgtggatt ccgtcccctg caaacgggcg tgggcggaga
     2701 gcctcggcat caaaaagacc cggctccttg cggatttctg gccgcatggc ggcgtggctc
     2761 aggcatacgg cgtctttgat gaagaaaagg gatactcagg gcgtgccaat atcgtcgtcg
     2821 atgaatcccg gcgcgtcgtc ttcgcgaagg cttacccggc ggcgacggtg ccggatatgc
     2881 aggatgtcat cgatgccctc cgggcacagg agcaggtgcc gcaggtgtaa tgtgagattc
     2941 cccatccggc cttcgttcca tcgcctgcgc agcgcttct
//
LOCUS       DAOSII010000210         2919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_29601_length_2919_cov_34.868715, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000210 DAOSII010000000
VERSION     DAOSII010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29601_length_2919_cov_34.868715"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..525)
                     /locus_tag="PLV90_10355"
     CDS             complement(112..525)
                     /locus_tag="PLV90_10355"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOF97558.1"
                     /translation="MLDNVVLDSCIIAAIFFPETISERAIEIVENHDCTTVDLAYIEV
                     ANVAWKRVRHSGPDPDIVRSVLANSQAFIRETCRVVPARDLIPAAYDLACGCGITIYD
                     ALFVAAAVQCGACLVTSDGRLHGAAKGVAAAQLIR"
     gene            complement(515..1147)
                     /locus_tag="PLV90_10360"
     CDS             complement(515..1147)
                     /locus_tag="PLV90_10360"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOF97559.1"
                     /translation="MRDSAEPGMMPDQPSILSIFQHLVSSLMLFGGMLAIATGFDPIS
                     KEREARSLKALLAHPVYRDEVINGKAPGGVAALAVPLTDGRAGFAGLPDVCDRRAPRS
                     SGFLCYNIPRQARACTYSQRLSMFIYPECIHDIMSTTSVYSIRIDSRIRKMIDELDDP
                     TWQDEVRAFIERSVRRRRKAQLLARAREAHRTLGEGPSAADLIREDRDAR"
     gene            complement(1164..1352)
                     /locus_tag="PLV90_10365"
     CDS             complement(1164..1352)
                     /locus_tag="PLV90_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97560.1"
                     /translation="MNDHIIHTRESMVAERIFFVAGKEFADYLTSRKFLVILALLLML
                     TLLGMHRGVDRYNQDLES"
     gene            complement(1886..>2919)
                     /locus_tag="PLV90_10370"
     CDS             complement(1886..>2919)
                     /locus_tag="PLV90_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOF97561.1"
                     /translation="EFSDLFTSRRFLLIFALYLIIAVVGMAQGLERYDYALSSYNEAQ
                     QAADEYRDLQELGASWLEMPGKPSILYVFSPMIGATMMLGAILAIAAGFDLVTREKES
                     RSLKTLLSHPVYRDEIIVGKAFGGGATLGIVVGLVLAVTTAVLLVFSIVPTAGEVVAI
                     LIFGLVSLLFLIAWFTVALAFSTAVRESGNALIFTLVVFFVISSLFPVLGALGGGFVA
                     GPPPQLPETPAVEVLPVMYVSNATGSYVVPGVDSGQQASARHDMLKEYQEELAAYTEK
                     KRTVTDVLTLLSPQKSYQAVTDVVSAPREMSLVDSLGSVWAGIAGLIAFPSIFFAAAY
                     TRFMRMDIR"
BASE COUNT          623 a          870 c          883 g          543 t
ORIGIN      
        1 cgatgcgatg cttgagcagg cgaaataccg ggggtttgga ggggagtagg gcggtcgccc
       61 ggatgggaag aagaacggtg cagcagatcg tggctgatac catctctgtg atcaacggat
      121 cagttgcgcg gccgccacac ccttcgccgc gccgtggagc ctgccgtcac tggtcaccag
      181 gcaggccccg cactggaccg cagcagcaac aaagagggca tcgtagatcg tgattccaca
      241 gccgcacgcc agatcgtatg ctgcgggtat caggtcacga gccggcacca cacggcaggt
      301 ttcccggata aatgcctgag aattggccaa cacggatctg acgatatccg ggtccgggcc
      361 ggaatgacga acccgtttcc aggcaacgtt tgcaacctcg atatatgcga gatcgaccgt
      421 ggtgcaatcg tggttctcta cgatctcaat tgctctctcc gagatcgttt ccgggaagaa
      481 gatggctgca atgatgcacg agtccagcac aacgttatcg agcatcacga tcctcccgga
      541 tcaggtctgc ggcagagggc ccttcaccca gggtgcgatg ggcttcccgc gcccgtgcca
      601 ggagttgggc cttgcgtctc ctccgtaccg atcgctcgat gaaggcacga acctcatcct
      661 gccacgtggg atcatccagt tcatcgatca tcttccggat ccgggaatcg atgcggatgc
      721 tgtaaaccga agttgtagac ataatgtcat gtatacattc cgggtatatg aacattgaga
      781 gcctctgaga gtacgtacag gcgcgggcct ggcgggggat attgtaacac agaaacccgc
      841 tggatcgcgg ggcccgccga tcgcacacat caggaaggcc ggcaaaccct gcccggccat
      901 cggtgagggg gaccgcgaga gccgcgaccc cgccgggcgc cttgccgttg atgacctcgt
      961 cacggtagac cggatgggcg aggagggcct tgagcgaccg ggcctccctc tcctttgata
     1021 tcgggtcaaa cccggtcgcg atcgcaagca tgcccccgaa cagcatcagg gaactgacca
     1081 gatgctgaaa tatggagagg atcgacggct ggtcaggcat catgccgggt tctgcggaat
     1141 ctctcatcgc ctgcagctgc cgattatgac tcaagatcct ggttgtaccg gtcaaccccc
     1201 cgatgcatgc cgagcagggt gagcatcagg agcagtgcaa ggatcaccag gaatttccga
     1261 ctggtgaggt agtcggcgaa ctcttttccg gccacgaaaa aaatccgttc tgccaccatg
     1321 gattccctcg tgtgtattat gtggtcgttc attctgtctg ccgccttggc gagagaaggt
     1381 acgtggcggc aaggagtgag gcggcagcac atggtccaaa cattttcttc tgtgtcatga
     1441 tggtttccac ctcattcatg cattgcccgg gaggaccgcg gaaccgtaac ggctatccct
     1501 tctgggcgcc gacagcccca ttgccctccg ggcacgctgt gggagccggg atacgatgcc
     1561 gatcgagttc cccggccccc acgggatact gcatgcggta ttgctgatcg agacatcggc
     1621 gttgcctccc tcaacgtttc tgtgctcact ctcgaattgt cggcggtgtc cccggctcac
     1681 cggttctcct cgatttacgg cgattgtctc tgttcacgaa cagtacagcc gatttccagg
     1741 gaaaatcgac cctgtgccct ctcacgggca cgggggtctg tttcgagcca aaattcgcga
     1801 catttgcacc cggcctggcc acccggcgga tcggcgatgc gtgtgcaccg atcctgtaca
     1861 taccgggtgg tcccatcttc caccgtcacc ggatatccat ccgcatgaac ctcgtatacg
     1921 cggccgcaaa gaagatcgac gggaaggcga tcagacctgc gatacccgcc cagacgctgc
     1981 cgagagaatc aacgagcgac atctcccggg gcgcagaaac gacatcagtc acagcctgat
     2041 aactcttttg cggcgagagg agcgtgagca catcggtgac ggttctcttt ttctcggtat
     2101 acgccgccag ttcctcctgg tactccttca acatatcatg ccgagcagaa gcctgttgcc
     2161 ctgaatcgac acccgggacg acatacgacc cggttgcgtt ggagacatac atcacgggga
     2221 gcacctcgac agccggtgtt tcgggaagtt gcggcggcgg gcctgcaacg aaccctccac
     2281 ccagcgcccc gagcaccggg aagagcgacg agatgacgaa gaagaccacg agggtgaaga
     2341 tcaacgcgtt gccgctctcc cggacagccg tcgagaacgc cagggcgacg gtaaaccagg
     2401 cgatcaggaa gaggagcgag acgagcccga agatcaagat agcaacgacc tcgcccgccg
     2461 tcggcacgat cgagaacacg aggaggacgg cggtggtgac ggcaaggaca agccccacga
     2521 cgatgcccag ggtggcgccg cccccgaacg ccttgccgac gatgatttca tcccggtaga
     2581 ccgggtgaga gaggagggtt ttaagcgacc gggactcctt ctcgcgggtg accaggtcga
     2641 acccggctgc aatggcaagt atcgcgccga gcatcatcgt ggccccgatc atggggctga
     2701 agacgtagag gatcgagggc ttacccggca tctcaagcca cgaggccccg agttcctgca
     2761 ggtcccggta ctcgtccgcc gcctgctgtg cttcgttgta tgaggagagg gcataatcgt
     2821 agcgctcgag tccttgagcc atcccgacca ccgcgatgat gaggtacagg gcaaagatca
     2881 ggagaaaccg tcggctcgtg aagaggtcgg agaactctt
//
LOCUS       DAOSII010000211         2917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_29622_length_2917_cov_35.090846, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000211 DAOSII010000000
VERSION     DAOSII010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29622_length_2917_cov_35.090846"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..500
                     /locus_tag="PLV90_10375"
     CDS             189..500
                     /locus_tag="PLV90_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOF97562.1"
                     /translation="MHPIIREKIPEIARIARRRRVKRLGIFGSATGDRFDPVASDIDF
                     LVEFEQMAPTEHAEAYFGLAEDLARLFCREIDLVELSAIRNPIFRESVKETCRDIYAI
                     A"
     gene            487..834
                     /locus_tag="PLV90_10380"
                     /pseudo
     CDS             487..834
                     /locus_tag="PLV90_10380"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
     gene            complement(1049..1210)
                     /locus_tag="PLV90_10385"
     CDS             complement(1049..1210)
                     /locus_tag="PLV90_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97563.1"
                     /translation="MKVLLRTPAGIKEVREADSDRVIPGDVLGDGSVVLELRDELSDD
                     DDLAMGPFD"
     gene            1454..1939
                     /gene="bcp"
                     /locus_tag="PLV90_10390"
     CDS             1454..1939
                     /gene="bcp"
                     /locus_tag="PLV90_10390"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOF97564.1"
                     /translation="MSELSAGMPAPDFCLPDADEETVCLADLRGAYAVVYFYPKDNTS
                     GCTLEARSFSDAREAFAREETPVFGISPDSTASHRRFSDKHNLSIRLLSDTERRTIEA
                     YGAWVTKKMYGREYMGVERSTFIIDPEGKIAAVWRKVKVKGHVDAVMATLEELKAGRP
                     A"
     gene            complement(1948..2607)
                     /locus_tag="PLV90_10395"
     CDS             complement(1948..2607)
                     /locus_tag="PLV90_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97565.1"
                     /translation="MPANREIIDAALEKARDLGADVAGAVPAGRLVDCPSAVAEGVCG
                     SPADRGTYIVLGLSHGPEHPELDLWEEGRGTPGDRILAGIGRNLARWLSECYGISARV
                     VPYQVEDGGIYLKDAACFAGIGRMGRNNLVIVPRYGPRVRFRAVWVDLDHPAEGLPGP
                     ALPCADCEGYCTRACPMGAFETGLYSRERCLARMNADKAAGKERIDHCRACELACPTR
                     D"
BASE COUNT          556 a          875 c          942 g          544 t
ORIGIN      
        1 tcgcggtcga cgaggaacag gcctttgccg ccatagagcg ggcggtcagg gggattgaag
       61 aagggagttt ctgacggcgg ggtgtcctgg atgggaggaa ctggagcggt gcggagacgg
      121 ctctccatcc actctcatcc gaaagagata atgcttgccg gaaggtaata tcgtcataga
      181 ggagtccagt gcatccaatc atccgcgaga agatccccga gattgcccgc atcgctcgac
      241 gccgccgtgt gaagaggctc gggatattcg gctcggcaac aggcgaccgg ttcgacccgg
      301 tagcaagcga catcgatttt ctcgtcgagt tcgaacagat ggccccgacg gagcatgcgg
      361 aggcctattt cggtctcgcc gaagacctgg cacggctctt ttgccgcgag atcgatctcg
      421 ttgaactatc ggcgatcaga aacccgatct tccgcgaatc cgtgaaagaa acctgcaggg
      481 atatctatgc catcgcgtga aggccggaaa tttctctacg acattgcaga agccgcggat
      541 tatattgtgc agttttccgc cgggaagacg tatgaggagt atcatgccga tcccatgctc
      601 cgttctgccg ttgaacggca gtttgagatc atcggtgaag cattgaacca gttcctccgg
      661 tgagaaccga gcctcaggga gaggatatcc gatgcgcccc tgatcatcgc gttcaggaat
      721 cgcctgatcc acgggtatgc gagcgtatcc gatgaggttg tctggggtgt cgtcgagagg
      781 tatctcccgg tcctcttgcg cgaagtgcgg gatctcttga gagagcagga gtaagaccgg
      841 acgaagaggg ttttacccgg ctctgcaaca aggatgtgtt gtgcctcata acgatggcta
      901 tgctccatcc attccgttgt gcgccatcta tgggatatac cgttcagtcc tcgcggtttg
      961 cacggggagt agcgctctac gaagagatct gccgggatca agccacatcg cgggcctgta
     1021 cgacctccgg atctttcagg ctcaacattc agtcgaatgg acccatcgcc aggtcgtcgt
     1081 cgtccgagag ttcgtcccgg agttcgagga cgaccgagcc gtcgcccagg acgtcgccgg
     1141 ggatgacccg gtcggaatcg gcctccctga cctcttttat ccccgccggg gttcggagaa
     1201 gtactttcat cgcggtatcc gctgcgaccg aaccgggtgc ccggccggcc gtacctacca
     1261 gagagtgatc ccggtcgggc aaaaaggttc cccgtcccgc tgcggcgccc gtggtgttcg
     1321 ccggacccgc tctcggttcg gggtcaacgg gggatacccg gggattcgat gcggcgaact
     1381 cttctttctt gccggaacgg ccatgcggtg cgattccggc acgcttttat cctcgccggt
     1441 gccagcctcc gatatgagtg aactctcggc aggtatgccg gcaccggatt tctgccttcc
     1501 ggatgcggac gaagagacgg tctgccttgc agacctgcgg ggggcctacg cggtcgtcta
     1561 cttctacccg aaggacaaca cgtcggggtg caccctcgag gcccggtcgt tctccgatgc
     1621 gagggaggcg ttcgcccggg aagagacgcc ggtctttggc atcagccccg actcgaccgc
     1681 gagccaccgg cggttctccg acaagcataa cctctctatc cgcctcctct cggacaccga
     1741 acgccggacg atcgaggcat acggcgcctg ggtgacaaag aagatgtacg ggagggagta
     1801 catgggcgtc gagcggagca cgttcatcat cgatccggag ggaaagatcg ccgccgtctg
     1861 gcggaaggtg aaagtgaagg ggcacgtgga tgcggtgatg gcgacgcttg aagaactcaa
     1921 agcagggcgc cccgcttgag gtaaggatca gtcacgggtc gggcaggcaa gctcgcaggc
     1981 gcggcagtgg tcgatccgct ccttgccggc ggccttgtcg gcgttcatcc gcgccaggca
     2041 ccgctcccgg ctatacagtc cggtctcaaa ggcacccatc gggcaggccc tggtgcagta
     2101 accctcgcag tcggcgcagg ggagggcggg ccccgggaga ccctccgcgg gatggtccag
     2161 gtccacccat accgcccgga accggaccct cggcccgtaa cgcggaacga tgaccaggtt
     2221 gtttctcccc atcctcccga tgcccgcgaa gcaggccgca tccttgaggt agatcccgcc
     2281 gtcctcgacc tggtagggga cgacccgggc gctgatgcca tagcactccg agagccatcg
     2341 ggcgaggttt cgcccgatcc cggcaaggat ccggtcgccg ggcgttcccc ggccctcctc
     2401 ccagaggtcg agctcggggt gctccgggcc gtgggagagg ccgaggacga tgtaggttcc
     2461 acggtcggcc ggagagccgc acaccccctc cgcaaccgcc gaagggcagt cgacgagccg
     2521 gccggcgggg acggcccccg cgacgtccgc cccgaggtca cgggctttct caagcgccgc
     2581 gtcgatgatc tcccgattgg caggcattgc agtacgtggt gggtcagggg tgaagaagga
     2641 tccggtgggg ttcccgggat ggagataaca gatctgttac ttttacggac agggttatat
     2701 atcccggtaa cagatagagg gttataccgt atccgggtgt ggagcatgaa cgagaccacc
     2761 cttgagcgcc agttatccag agtccccccg accttcatca gcgtcacggg acccctgggg
     2821 aacaagaagg catgggcgat cttcatcgca ctcctgcaga gcgatgaagg gctccggttc
     2881 aaccagatcc gggacctctt tgaggcggag cccccgg
//
LOCUS       DAOSII010000212         2915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_29651_length_2915_cov_45.253147, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000212 DAOSII010000000
VERSION     DAOSII010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29651_length_2915_cov_45.253147"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..1613)
                     /locus_tag="PLV90_10400"
     CDS             complement(204..1613)
                     /locus_tag="PLV90_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HOF97566.1"
                     /translation="MPRIYLGKIPLRWCDTCHTPVLSGTCACGAPARPVAVTPPGDAR
                     PAFPDDVDRVNRVFCEHFGAPLIPEGHLSLLNKVPADDRMDEIVLGGAVVGAIRYIPG
                     EQRWEPLPRPNAAAFLRPTKRYVVVDDGAIPSIRDGGASVLAPGLLSIDPAVAEGDEV
                     FILTEAGECIGVGRAKVDAAVATTMERGLVVRTRRNVSSACVPGEATWEDAVAANERI
                     LAEYEAASIQFVRDVAEKNPERPTVSYSGGKDSLATLLIVLKALGPVPLLFADTGLEF
                     PETCGNVEAVAERYGLEVLRVCDEAAFWSEFERSGPPAVDNRWCCRVCKLHPLGRLID
                     EHFGECLSFIGQRKYESVRRMQSRRVWRNDRVPQQLSAAPIQQWTAMHVWLYIFREKA
                     PYNRLYERGLDRIGCFMCPSSDLATFALIRESCPELSEMWLAKLAGWQEKQGLPRDWV
                     ERGLWRKRGGADEEEDSYN"
     gene            2179..2637
                     /locus_tag="PLV90_10405"
     CDS             2179..2637
                     /locus_tag="PLV90_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOF97567.1"
                     /translation="MHEGRIAGVALGAAFALAVGLYAASGHLAPGWAPPGCAVLAIGV
                     VLPFFTSGAEVPSVRVARKPMRKIEILGLLIFILMAVVMLAVGAALFCDWLAEPGSAP
                     VGTPVELGPVSGELGVLGTIPVMALMIGVEVVGGFALIALYMLSAVGREG"
BASE COUNT          503 a          916 c          925 g          571 t
ORIGIN      
        1 gctaccttac ttctcgtcgc ccgcgccttc ggcgctccgc ccctaccgcg gggtggccgg
       61 gggcagtgcc cgggtgttgt cggcggggag ccgtgctgaa aagagatatg gcgccggcga
      121 cgggaagggg gtcgtcaccg gatccatcgt gattgccgcg accgcccctg cctgttcaac
      181 cccggaggct gccgggcccg caatcaatta taactatcct cttcctcgtc ggcgcctccc
      241 cgcttgcgcc acaacccgcg ctcgacccag tcacggggga ggccctgctt ctcctgccat
      301 ccggcaagtt ttgcgagcca catctcagag agttcggggc aggactccct gatgagcgca
      361 aacgtcgcaa ggtcgcttga agggcacatg aaacatccga tgcggtcgag cccgcgctcg
      421 tagaggcggt tgtagggggc tttctcgcgg aagatgtaga gccagacgtg catcgcggtc
      481 cactgctgga tgggggccgc cgagagttgc tgcgggacac ggtcgttccg ccagacccgc
      541 ctgctctgca tccgccgcac cgactcgtac ttccgctgcc cgatgaacga gaggcactct
      601 ccaaagtgtt cgtcgatcag gcggccgagg gggtggagct tgcagacccg gcaacaccag
      661 cggttatcga cggccggcgg gccgctcctc tcaaactcgc tccagaacgc tgcctcatcg
      721 catacccgga ggacctcaag cccatagcgt tctgccaccg cctccacgtt cccgcaggtc
      781 tcggggaact caagccccgt atcggcaaag aggagcggaa ccgggccgag cgcttttaag
      841 acgataagca gggttgcgag gctgtctttc ccgcccgagt aggagaccgt gggccgctcc
      901 gggttcttct ccgcaacatc ccggacgaac tggatgctcg cggcctcgta ctccgcaagg
      961 atgcgttcgt ttgccgcaac ggcatcttcc cacgtcgcct cgcccgggac acaggctgat
     1021 gaaacgttcc tgcgcgtccg gacgacgagc ccccgctcca tcgtcgtggc gacggcggcg
     1081 tcgaccttcg ccctgccgac accgatgcat tcgccggctt ctgtcagtat gaagacctca
     1141 tcgccttcgg cgacggcagg atcgatcgag aggaggccgg gtgcgaggac gcttgccccc
     1201 ccgtcacgga tggatgggat cgccccatcg tcgacgacga cgtagcgttt cgtcggccgc
     1261 aggaaggcag cggcgttcgg acgcgggagg ggctcccagc gctgttctcc gggaatatag
     1321 cggatcgcgc ccacgaccgc gccgccaagg acgatctcgt ccatgcgatc gtcggcggga
     1381 accttgttga ggagcgacag gtgcccttcc ggtatcagcg gcgctccgaa atgctcgcag
     1441 aatacgcggt tgacccgatc gacgtcatca gggaacgcgg ggcgggcatc tcccggtggc
     1501 gtgacggcta ccgggcgggc tggcgctccg caggcacagg ttcccgagag caccggggta
     1561 tggcaggtat cgcaccagcg aagcgggatc tttccgaggt aaatgcgagg cattcttata
     1621 cccattgggg agaaatagat aaaagtcttg gcgcacttcc gatggttttc gcaatctccc
     1681 tccggagagt attctgctgg agtctgccgg cgacggccct ttatcaaggc aggaatcgct
     1741 ctcgccttac gacgaactcc tgttgccggt attggagggt cagggcatat cattaaatat
     1801 atcctctgcc tcatagtatt atcatgttca atcatgtagg atcgtgctcg ccgccttctt
     1861 gccggcccgc cgacgattct gcctgatgaa aggagagata ccgtatgtac cgggtgatgt
     1921 tcacgacccc cttcccgccg accgttcagg tcgcaggtgc agtgtctggc tttcccttcg
     1981 caacgaccgg ttatccggtc aagcgggaag ccacaactac cgggaggcag ggtccgcgtg
     2041 ccgggttgtc cgcggagcct gcaccttcgg ttcgttctgc atggaaggcc actctcgtga
     2101 agcgcccggc ggccggtttc tcggccgcat ctctcgtatt cggccagctc ttcggagatg
     2161 gttcccggag tgtgctgcat gcatgagggc cgaatagccg gcgtagccct cggagccgcc
     2221 ttcgccctgg cggtcgggct ctatgcggcg agtggtcacc ttgcgcccgg gtgggcgccc
     2281 cccgggtgtg cggtgcttgc gatcggggtt gtcctgccgt tcttcacctc gggcgcggaa
     2341 gtgccgtcgg tacgggtcgc acggaaaccg atgcggaaga tcgagatcct cggtctgctc
     2401 atcttcatcc tcatggcggt cgtcatgctc gcggtcggtg cggcactctt ctgtgactgg
     2461 ctcgccgaac ccggttcggc ccccgtggga acaccggtcg agctcggacc ggtatcgggg
     2521 gagctcgggg ttctcggcac gatcccggtc atggctctca tgattggtgt cgaggtggtg
     2581 gggggattcg cgctgatcgc cctctacatg ctgtccgccg tcgggagaga ggggtaggtt
     2641 ttaccccatc ccggcgcccg atttttcctg gttccgcatt gcagcatccc tctggtgcga
     2701 gcggcgatcg ccccccggcc ggtcggccat aaataacgct taattttcgc ctggtttatg
     2761 gaaacactga ctgatcaagc gtaaaatata tatccggacc ggttaacatc agatatatcc
     2821 cacctcggga gggaggtgtg tcatttatgg caaaggataa gaagccccag gagaaaaagc
     2881 cccaggagaa gaagcaggac aagccccagg acaag
//
LOCUS       DAOSII010000213         2904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_29774_length_2904_cov_40.528256, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000213 DAOSII010000000
VERSION     DAOSII010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29774_length_2904_cov_40.528256"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="PLV90_10410"
     CDS             complement(<1..1199)
                     /locus_tag="PLV90_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HOF97568.1"
                     /translation="MESCREIELEGHIIDSGIMTLVFDRIMDMGGEFEILTFNVGKQK
                     TDTSYARLRVTAPTGRQLDAILSELHRLGARTPEISDVTLVPAEGDRILPKGFYSTTN
                     HPTFVKYRDEWLPVEHIEMDCHIVIDEAEKRAFCKPIAKIKAGDAVVVSEAGVRVVYP
                     ERPRKVSTFEFMHGTVSSERPSEAIIAKIAREILAMKEEGGKIALVGGPAIVHTGAAG
                     ALAAMIRKGYIDVLFAGNALATHDIESNLFGTSLGMDVRTGTLVTGGHRHHIHTISEI
                     MRAGSIKEAVDRGIVTGGIMYECVKRNVPFVLAGSIRDDGPLPDTITDVVVAQDAMRC
                     HIRDIGMVLMVGTLLHSIAVGNCLPSYVKTVCVDINPASVTKLMDRGTTQAIGVVSDA
                     GTFLPLLL"
     gene            complement(1325..1930)
                     /locus_tag="PLV90_10415"
     CDS             complement(1325..1930)
                     /locus_tag="PLV90_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOF97569.1"
                     /translation="MLALIMAGGQGSRLRMGEKPLVTICGKPMLAYVIDAFASAGHEV
                     VVVASHRTPFTKNWCRAQGIVLYEAEGLGYVEDICAAADDLGEAGTPFFTCVSDLPCL
                     APDIIAGVEEAWRRAKTPACSTWVPRSLCEEHGCRTQYTETVDGVPACPAGINILTAG
                     GFDGLEEELCLLLHDRRLAFNVNTREELALVQGYLCRKRVP"
     gene            complement(1918..>2904)
                     /locus_tag="PLV90_10420"
     CDS             complement(1918..>2904)
                     /locus_tag="PLV90_10420"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOF97570.1"
                     /translation="LPERMEHGGRVRWHCMREGGELIDFSANINPYPPIIPWTPDPSA
                     LKDYPDDRYEALKEVIGSTFGRDAAEITVGNGSVELIRAFCSAVLGEGDTACIQPPTF
                     AEYEMAARLAGARCTADVSGAAVRFLCNPNNPTGRLSTRPEILGALDETANEGGYLFV
                     DEAFIELADPRQSVVDVSHEHLFTLRSLTKSFTVPGIRFGYGFGTPELIEQVETMRLP
                     WTVNAFAEAFAIEAFRHYDLLERSRERIAREREWLCGRLTDLGLAYEPPSANYVLMEV
                     PLEAGVLVARLLSHGILARDCRSFGLPRHIRVAVRTREENRQLIEAFEACLP"
BASE COUNT          516 a          942 c          917 g          529 t
ORIGIN      
        1 aggagcagcg ggaggaacgt cccggcatcg ctcacgacgc cgatcgcctg cgtcgtgccc
       61 cggtccatca gtttcgtcac cgacgcgggg ttgatatcga cgcagaccgt cttgacgtac
      121 gaggggaggc agttcccgac cgcaatcgag tggaggagcg tcccgaccat cagcaccatc
      181 ccgatgtcgc ggatgtggca gcgcatggcg tcctgcgcca cgacgacatc cgtgatcgta
      241 tcggggagcg gcccgtcgtc ccggatagaa ccggcgagga cgaacgggac attccgcttc
      301 acgcactcgt acatgatccc cccggtcacg atccccctgt cgaccgcctc tttgatggat
      361 cccgcccgca tgatctcgct gatggtgtgg atgtggtgcc ggtgcccgcc ggtgacgagc
      421 gtccccgtcc tgacgtccat cccgagcgac gtgccaaaga ggttggactc gatatcatgc
      481 gtcgcaagag cgttccccgc gaagagcacg tcgatgtagc ccttgcggat catcgccgca
      541 agggccccgg ccgccccggt gtggacgatc gccgggccgc cgacgagcgc gatctttccc
      601 ccctcctcct tcatcgcgag gatctcgcgc gcgatcttgg cgatgatcgc ctcgctcggc
      661 cgttccgacg agacggtccc gtgcatgaac tcaaacgtgc tgaccttccg cggccgctcg
      721 gggtagacca cacgcacccc ggcctcgctc acgacgacgg catcgccggc ctttatcttc
      781 gctatcggtt tgcaaaaggc ccgcttctcc gcctcatcga tcacgatgtg gcagtccatc
      841 tcgatatgct cgaccggcag ccattcatcc cggtacttca cgaacgtcgg atgattggtg
      901 gtcgagtaga accccttcgg gaggatccgg tcgccctctg ccggtacaag ggtgacgtcg
      961 ctgatctcgg gggtgcgtgc gccgagccgg tgcagctcgg agaggatggc gtcgagttgc
     1021 cgcccggtgg gtgccgtcac gcgcagccgc gcgtaactcg tatccgtctt ctgcttcccg
     1081 acgttgaacg tgaggatctc aaactcccca cccatatcca tgatcctgtc aaacacgagg
     1141 gtcatgatgc ccgaatcgat gatatgcccc tcgagttcga tctcccggca tgactccata
     1201 taaagagatc agcgatggtc cgataataga gtttctccca aaaaacgatt gttcgggaga
     1261 gatgctcccg tggagcgaag ttcgggaaga ccggaggctt ttctgtgcgg ccgtccgccg
     1321 gaggttaggg gacccgcttc cggcagaggt acccctgcac cagcgccagc tcctcgcgcg
     1381 tgttgacgtt gaaggcgagc cggcgatcgt gcaggagaag gcagagctcc tcctcaaggc
     1441 catcaaagcc gcccgccgtc agtatattga tccctgccgg gcaggccggc acgccgtcga
     1501 ccgtctccgt atattgcgtg cggcagccat gctcttcgca gagactgcgc ggcacccacg
     1561 tggagcatgc cggcgttttt gcccggcgcc aggcctcctc gacgccggcg atgatatcgg
     1621 gggcaagaca gggcaggtcg gagacgcagg tgaagaaggg cgttcctgct tctccgaggt
     1681 catccgccgc cgcacagata tcctcgacgt agccgagccc ttcggcctca tacaggacaa
     1741 tcccctgcgc ccggcaccag tttttggtaa acggcgtccg gtgcgaggcg accacgacca
     1801 cctcgtgccc tgcggatgcg aaggcgtcga tgacgtaggc gagcatgggt tttccgcaga
     1861 tggtgacgag cggtttctcg cccatccgga gacgggagcc ctgccccccg gccatgatca
     1921 gggcaagcat gcttcgaatg cctcgatgag ttgccggttc tcctcgcggg tccgcaccgc
     1981 tacacggatg tggcggggta gcccgaacga ccggcaatcc ctggcgagga tgccgtggga
     2041 gaggagccgc gcgacaagca cccctgcctc cagcgggacc tccatcagga cgtagttcgc
     2101 agatgggggt tcgtatgcga gaccgaggtc cgtaaggcgg ccgcagagcc attcgcgttc
     2161 ccgggcaatc cgctcccgcg aacgttcgag cagatcgtag tgccggaacg cctcgatggc
     2221 gaaggcctcg gcaaacgcat tcaccgtcca ggggaggcgc atggtctcca cctgctcgat
     2281 gagttcgggc gtgccgaacc cgtatccgaa ccggatgccc ggcacggtga agctcttcgt
     2341 cagggagcgc aaggtaaaga ggtgctcgtg ggagacgtcg acgacgctct ggcgcgggtc
     2401 ggcgagttcg atgaacgcct catcgacgaa gagataccct ccctcattcg ccgtctcatc
     2461 aagcgccccg aggatctcgg ggcgggtaga gagcctgcct gtcgggttgt tcgggttaca
     2521 gaggaaccga acggcggcac cgctcacgtc ggccgtgcac cgggcgccgg cgagcctcgc
     2581 cgccatctcg tactcggcaa atgtcggcgg ctggatgcag gcggtatccc cctcgccgag
     2641 cacggcggag cagaacgcgc ggataagttc gacagatccg ttgcccacgg tgatctcagc
     2701 cgcatcgcgc ccgaacgtgc tgccgatcac ctctttgagc gcctcataac ggtcgtcggg
     2761 gtagtctttc agtgccgaag ggtccggtgt ccaggggatg atagggggat atggatttat
     2821 gttagcactg aaatcgatca gctcgccacc ctctcgcatg cagtgccagc gtacccggcc
     2881 gccgtgttcc atcctttcag ggag
//
LOCUS       DAOSII010000214         2900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_29832_length_2900_cov_40.600703, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000214 DAOSII010000000
VERSION     DAOSII010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29832_length_2900_cov_40.600703"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PLV90_10425"
     CDS             <1..552
                     /locus_tag="PLV90_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97571.1"
                     /translation="PTPTVTPGVPAKSMKLELYKGWNFVSIPRPLSPGNNTAIEVFRD
                     VETGGRPIYTYTPENGFEPLGADTTLKVLDGYWVYSTEAMTVQLNFSTNPVMVPASKM
                     LSPGWNAIGYSDLTPRSANESLISVEDSWVSVVGYNAKNQNYQPALINGLTGAHGENQ
                     KLLPTKGYWLFMREDGTLAAINA"
     gene            complement(841..1116)
                     /locus_tag="PLV90_10430"
     CDS             complement(841..1116)
                     /locus_tag="PLV90_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAC family transcription factor"
                     /protein_id="HOF97572.1"
                     /translation="MTNEKEGDYCTVCGGIKPEAIKIKSVLVDGKATGINQLDFIVAG
                     VRDLHLADDAAIRAELLRRAGEFNYIPTKKKEAYADALLQEYKEVTR"
     gene            complement(1113..1460)
                     /locus_tag="PLV90_10435"
     CDS             complement(1113..1460)
                     /locus_tag="PLV90_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOF97573.1"
                     /translation="MAKEKRAELQGKVLRLPELVAYQDGTVASRMIVNNRSGSITLFS
                     FDEDEGLSEHTAPYDAVVTILDGECEVWIAGETHQMSAGETIIFPADVPHALSAITRF
                     KMSLTMIRGREDT"
     gene            complement(1514..2287)
                     /locus_tag="PLV90_10440"
     CDS             complement(1514..2287)
                     /locus_tag="PLV90_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOF97574.1"
                     /translation="MVIESIPKVVGFIYGLLAFIALVGLWYSGRFTRRRALPFLVISA
                     LLGFLVFAPVFPYQLQMVALRDTVGLGSPIPMALGALLVFIALAFAFGRVICGQVCPA
                     GAVQELMYRVPVAKRGSAERQTPVVIRAGVFVVFAVAALGLSADLLGLIGLPAFFHLA
                     VGSVSFFVFLGIVLLSAVVYRPFCRYVCPYGALLAPAASKALFRLRRTDACIGCRKCE
                     RACPTGEADPAAGLGECYLCGRCTDVCPVEGALVYGRAR"
     gene            complement(2295..2633)
                     /locus_tag="PLV90_10445"
     CDS             complement(2295..2633)
                     /locus_tag="PLV90_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOF97575.1"
                     /translation="MKIVDVAKEPISKTPHNVDARKVYDTEHATAVVITLAPGESLKK
                     HITPVDVFFYVLEGTGVVEIGDERAEVGKDHLVESPAKIPHLWANESKAPFKVLVVKV
                     PRPTTGTRLL"
BASE COUNT          580 a          926 c          852 g          542 t
ORIGIN      
        1 ccgacaccga cggtgacgcc gggagtgccg gcaaagagca tgaagctcga gctgtacaaa
       61 ggctggaact tcgtctccat cccaaggccg ctctctccag gtaacaacac cgcaattgag
      121 gtattcaggg atgtcgagac cggtggaagg ccgatctaca catacacccc ggagaacggc
      181 tttgagcccc tgggcgcgga tacgaccctg aaggtccttg atggctactg ggtctactcg
      241 accgaggcca tgaccgtgca gctgaacttc agcaccaacc cggtgatggt tcccgcatcc
      301 aagatgctct ccccgggatg gaatgccatc ggctactccg acctgacacc gcggagcgca
      361 aacgaatcgc tcatatcagt ggaagacagc tgggtctctg ttgtcggtta caacgcaaag
      421 aaccagaatt accagccggc gctcatcaac ggtctgacag gagcacatgg tgaaaaccag
      481 aaactcctcc cgaccaaggg ctactggctg ttcatgcgcg aggatggcac actggctgcc
      541 atcaacgcct gacccttttt ttgatgcttc ccgggaccat ttcatcctga aacccgcgct
      601 ttccaaccga acgcccggtc tgttttacaa aaaagagcgt gaacccgtgc acctcatacc
      661 gggtacccgc accgccatcc gcatgccggc acctcgcacg ttgccatgaa ggccgcactg
      721 gagagcggct caccgccaac ccgatcgttg tgctcttttc ggcggtgcaa gcgccggcag
      781 cgggggtcaa aggagagaac cccgcgcgaa ggttcacgcg ggggagcgta ctccggcgcc
      841 tcaccgggta acttccttat attcctgcaa cagggcgtcg gcgtacgcct ctttcttctt
      901 cgtcggtatg tagttgaact ccccggccct ccggaggagt tcagcccgga tcgcggcatc
      961 atccgcaagg tgcaggtccc gcacgccggc gacgatgaag tcgagctggt tgatgccggt
     1021 cgccttccca tccacgagga ccgacttgat cttgatcgcc tccggcttga tcccgccgca
     1081 gacagtacaa taatcgcctt ctttctcgtt ggtcatgtgt cctctctccc ccggatcatc
     1141 gtgagcgaca tcttgaaccg tgtgatggca gagagggcgt gcgggacatc tgccgggaat
     1201 atgatcgtct cgcccgcgct catctggtgc gtctcgcctg cgatccagac ctcgcactcg
     1261 ccgtcgagga tagtcaccac cgcatcgtag ggcgcggtgt gctccgagag cccttcgtcc
     1321 tcgtcgaatg agaagagggt gatgcttccc gaccggttgt tgacgatcat cctgctcgcc
     1381 acggtcccgt cctggtaggc gacgagttcc ggtaaccgga gaacctttcc ctgtaactct
     1441 gcacgtttct cttttgccac tggtacacca gcctctcttc tttattctgg cgtccttcgc
     1501 ttatttaccc ttctcatctc gcccgcccat acacgagcgc cccttccacc gggcagacat
     1561 ccgtacaccg gccgcagagg tagcattccc cgagaccggc ggcagggtcc gcctccccgg
     1621 tcgggcatgc ccgctcacac ttccggcacc ctatgcaggc atccgtccgc ctgagccgga
     1681 agagcgcctt tgatgccgca ggtgcgagga gggcgccgta tgggcagacg tagcgacaga
     1741 acggccggta gacgacggcg gagaggagga cgatgccgag gaagacgaag aacgacacgc
     1801 tcccgaccgc gaggtggaag aaggcaggta gaccgataag cccgaggagg tctgccgaga
     1861 gaccgagagc ggcgacggcg aagacgacga agacgccggc ccggatcacc accggcgtct
     1921 gcctctcggc gctaccgcgc tttgctaccg gcacacgata catgagttcc tggacagccc
     1981 ccgccgggca gacctgcccg cagatgaccc ggccgaaggc gaaggcaagg gcgatgaaga
     2041 caaggagcgc cccgagcgcc atagggatgg gtgacccgag gcccaccgta tcccggaggg
     2101 cgaccatctg cagctggtag gggaagaccg gcgcgaatac caggaacccg agaagcgccg
     2161 atatgaccag aaacggcagc gcacgccgcc gggtgaaccg gccggaatac cagagcccga
     2221 cgagggcgat aaaagcaagg aggccgtaga tgaaccctac gaccttcgga atcgactcga
     2281 tgaccattac atcctcagag cagcctcgtc ccggtcgtcg ggcgcgggac cttcaccacg
     2341 agcaccttga agggggcttt gctctcgttt gcccagaggt gcggtatctt tgccggactc
     2401 tcgaccaggt ggtccttccc gacctctgcg cgctcatcgc cgatctcgac gacccccgtc
     2461 ccctcaagga cgtagaagaa gacgtccacc ggggttatat gcttcttcaa cgactctccc
     2521 ggcgcaaggg tgatcaccac cgcggtcgca tgctcggtgt cgtagacctt ccgggcgtcg
     2581 acgttgtgcg gggttttgct gatcggttct tttgctacat caacgatctt cataacactc
     2641 atctccaatg gtatacggga ctctcgtgat atttatcttt gctgagttga aggggcggag
     2701 cgggaagacc ccctgcacaa gcgggggggt agttcaccct ttatagtttg tccagtagtt
     2761 ttcgaggcac tcctgccgcc tccgcttctg ccggtgggcc cgcatctcgg gataggtcgg
     2821 ttcaacgctc tggaccgccg ggcaataggg gcagagggcg aaagccacgt cgtcggcctt
     2881 atccctgacc gggcagaggt
//
LOCUS       DAOSII010000215         2893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_29930_length_2893_cov_40.960536, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000215 DAOSII010000000
VERSION     DAOSII010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29930_length_2893_cov_40.960536"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..2120)
                     /gene="cooS"
                     /locus_tag="PLV90_10450"
     CDS             complement(210..2120)
                     /gene="cooS"
                     /locus_tag="PLV90_10450"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HOF97576.1"
                     /translation="MTMESNRISYHDSARTVYERLKKDGMSNVWDRYEAQGLGGDPDR
                     RCAFCQAGARCDFCSNGPCRADATRNWRGVCGISVDGMAMRMMLLRNVMGASNYHFHA
                     AQAVRTLRATAEGKTPFSIVEPEKLRAFAGRLGIDTTGSDSDVALRLCDFVEEDFHRF
                     THEPSRIVEHLAPPERKEVWKKLNLFPGGIYGETIVDTASTLTNVDGWFASHAMRAMR
                     LGVAMAYQSQIVLEYIQDILYGIPRPHPMRVDLGVLDPDYVNILPNGHEPFLGFALID
                     LARTGEWQAKARAAGAKGLRIIANIETGQELIQRREMDDVFYGYTGNWIMQEAVLATG
                     AVDVFAADMNCSLPLDPLYAEKYHFKLIPVSEVVVFEGGGERLDYVPEEAKEQAEALL
                     DMGIENFKERHSMIEPIRGLPVREAVVGFAPESIVAALGGSLDALLGAIKDGTIRGIV
                     GLVSCTTLRDTGQDVHTVAVAERLIARDILVLGMGCGVAALQVAGLCSPEAKEKAGPG
                     LKGLCTALGVPPVLGFGTCTDTGRCADLLLAVSEALGGVPIPDLPVAVAAPEYMEQKA
                     TIDALFALALGLYTYVNPVPTVTGGPDLVKLLTEDLRWITGGVLRVETDAEAAVEGIA
                     GHIEGRRKILGL"
     gene            complement(2166..2510)
                     /locus_tag="PLV90_10455"
     CDS             complement(2166..2510)
                     /locus_tag="PLV90_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HOF97577.1"
                     /translation="MIRIKRIYEEPSADDGLRVLVDRLWPRGISKARAQVDRWEKDIS
                     PTTELRHWFGHDPAKWEEFLQRYRAELEDKKELLSQLREEADERTVTLLYAAKDEEHN
                     NAVALKRYIEEG"
     gene            2662..>2893
                     /locus_tag="PLV90_10460"
     CDS             2662..>2893
                     /locus_tag="PLV90_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97578.1"
                     /translation="MTRLERLLILTLAIGCVALLSGVVAQEGNATNQTANQTANQTTG
                     VNVTITSPEEGASIPAGNVTVEVNLTNFTLVEP"
BASE COUNT          512 a          974 c          899 g          508 t
ORIGIN      
        1 gatccagtgc tacgatcacg cgctggagat agaccccgga gatactctcg caaagagcaa
       61 cagagaaggt gcattgaaaa agaggggtgc aacagcaaaa gagagtgata gaggggcctc
      121 gatataaggg cgcacgcacc gaagacctac cggaatcgcc tcgccagaga ggggccgttt
      181 gagcagaagc cccctgcccg tcccgcccct cacagcccga gaatcttgcg cctcccctcg
      241 atgtgccctg ctatcccctc caccgccgcc tctgcatccg tctcgacgcg gagcaccccg
      301 ccggtgatcc accggaggtc ttcggtgagg agtttgacga ggtccggccc cccggtcacc
      361 gtcgggaccg ggttgacgta ggtgtagagg ccgagcgcga gcgcaaacag ggcgtcgatc
      421 gtcgctttct gctccatgta ctccggtgcg gcgacggcga ccgggaggtc ggggatgggg
      481 acgccgccga gggcttcgga gaccgcgagg aggaggtcgg cgcaccggcc ggtatcggtg
      541 cacgtcccga atccgagcac cggcgggacg ccgagcgccg tgcagagccc tttgagcccc
      601 gggccggcct tctccttcgc ctccggagag cagagcccgg cgacctggag ggccgcaacc
      661 ccgcagccca tcccgaggac caggatgtcg cgggcgatca gtctctccgc gaccgcgacg
      721 gtatggacgt cctgcccggt atcccgcagc gttgtgcagg agacaagccc cacgatgccc
      781 cggatggtgc cgtccttgat cgctccgagc agcgcatcaa ggctcccccc gagcgccgca
      841 acgatgctct cgggcgcaaa cccgaccacc gcctcccgca cgggaaggcc ccggatcggc
      901 tctatcatgc tatgccgctc cttgaagttc tcgatcccca tatcaagaag cgcctccgcc
      961 tgctcctttg cctcctccgg gacgtaatcg agccgctcgc ccccaccctc gaagaccacg
     1021 acctcgctca ccgggatcag tttgaagtgg tacttctcgg cgtagagggg atcgagcggg
     1081 agcgaacagt tcatatcggc cgcaaagaca tcgaccgccc cggtggcaag caccgcctcc
     1141 tgcatgatcc agttccccgt gtagccgtag aagacgtcgt ccatctcccg gcgctggatc
     1201 agttcctgcc cggtctctat gttcgcgatg atccggaggc ctttcgcccc cgccgcccgc
     1261 gcctttgcct gccattcacc ggtgcgggca aggtcgatga gggcaaaccc gaggaagggt
     1321 tcgtggccgt tcgggaggat gttgacgtag tcggggtcga gcaccccgag gtcgacccgc
     1381 atggggtgcg ggcgggggat gccgtagagg atatcctgga tgtactcgag cacgatctgg
     1441 ctctggtagg ccatcgcgac gccgaggcgc atcgcccgca tggcgtggct cgcaaaccag
     1501 ccgtcgacgt tcgtgagggt cgaggcggta tcgacgatcg tctccccgta tataccgccc
     1561 gggaagaggt tgagcttctt ccagacctct tttcgctccg gcggggcaag gtgctccacg
     1621 atccggctcg gctcgtgggt gaaccggtgg aagtcctcct cgacaaagtc gcagagcctg
     1681 agggcgacat cagaatcgct cccggtcgta tcgataccga gccggccggc aaacgcccgg
     1741 agtttctccg gttcaacgat ggagaagggg gtcttgccct cggcggtcgc ccggagggtc
     1801 ctgaccgcct gcgccgcgtg gaagtggtag ttcgatgctc ccatgacgtt tcggagcagc
     1861 atcatccgca tcgccatgcc gtcgacgctg atcccgcaga ccccccgcca gttccgggtt
     1921 gcgtcggccc ggcaggggcc gttcgaacag aagtcgcagc gtgcccccgc ctggcagaag
     1981 gcacaccgcc ggtcggggtc gccgccgagc ccctgggcct cgtagcggtc ccagacgttc
     2041 gacatgccgt ccttcttgag ccgctcgtag acggtccggg ccgagtcgtg gtaggatatc
     2101 ctgttgctct ccatggtcat gagcgggtga ctgagccggg accatgaaga acgtatcggt
     2161 gcggcctatc cttcctcgat ataccgcttc agcgccacgg cgttgttgtg ctcttcgtct
     2221 ttcgcggcgt agaggagcgt gacggtcctc tcatccgctt cctcgcggag ctgagagagg
     2281 agctccttct tatcctcaag ctcggcccgg tagcgctgca ggaactcctc ccactttgcc
     2341 gggtcgtgcc cgaaccagtg gcgcagttcg gtcgtcggtg aaatatcctt ctcccaccgg
     2401 tcgacctgcg ccctcgcctt cgatatcccc cgcggccaga gccggtcgac gagaacccgg
     2461 agcccgtcgt ctgccgatgg ctcttcgtag atccttttta tgcgtatcat aagcgccgat
     2521 acgattgacc cctacttaac cctatccacc gaagcaaccg ggcccgtaca gcagaggatc
     2581 tccgccggca ggctctctcc ggggcgagag gtgcaacctt ccgaaaggta tatcatgcat
     2641 gcttccatct ccccgccccg tatgacgaga ttggaaagac tcctgatcct cacccttgcg
     2701 atagggtgcg tcgcgctcct ctccggggtg gtcgcccagg aaggaaacgc gaccaaccag
     2761 actgcaaacc agaccgcaaa ccagaccacc ggcgtgaacg tcacgatcac gtcgccggag
     2821 gaaggcgcga gcatccctgc cgggaacgtg accgtcgagg tgaacctgac gaacttcacg
     2881 ctggtcgagc cga
//
LOCUS       DAOSII010000216         2852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_30503_length_2852_cov_45.478727, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000216 DAOSII010000000
VERSION     DAOSII010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30503_length_2852_cov_45.478727"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PLV90_10465"
     CDS             complement(<1..533)
                     /locus_tag="PLV90_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5714 domain-containing protein"
                     /protein_id="HOF97579.1"
                     /translation="MEHRAGCLICGRDILYSEEPRDLPCAVCGDIRSTTAACGAGHFV
                     CDRCHSLEALDLIEEFCIATDLEDPLAVACILMRCPAVRMHGPEHHFLVPASLIAAYC
                     NHRGEPGRKAPLVRQARARAGMVPGGFCGSHGTCGAAIGTGIFVSLITGSTPLKKQEW
                     SLANQMTARSLMTIARHG"
     gene            complement(523..2379)
                     /locus_tag="PLV90_10470"
     CDS             complement(523..2379)
                     /locus_tag="PLV90_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF97580.1"
                     /translation="MSITDCYRRSIAFTRDILRQPAKTGGGLRFSDIAFFLRFARPIR
                     RAGALALVTTALVSAEKTVLPLSLKIFVDNILLGEAVAPDALLAGTGGILSSLNILVA
                     ALLVLGLLTGATDLARNYWTARFREGYAFNLQTALFEHVLGFPLSYFKSQQTGYIMSR
                     LSDDVQILQYTVSQYLPQVVANALYAAFTLVILGALNIRLTLVVLAFIPLYILVNAVF
                     IRRIRAAVHRERERQAYVSRDLAEVISGIDTVKSHAAEDREVHRVSGTLRNMVDARIR
                     STMLSAFSEYFRVGTMSLMLIAVIWFGGNDVLAGAMTVGDFVAFAVYIMTFAPTVSTF
                     FAFPVVMQPAATSAARLRELFAMQGEPGALPDAGGFVPDAVQGRIEFSRVWFGYGEDT
                     PVLRDAQFVIRPGEVVAVVGRTGAGKTTLINLILRAFSPEKGTITLDGRDLSTLDPRW
                     LRRQISIVSQDLFLFHTTIEENIRYSRPDATAGDVVEAAEKAQIHDDIVRLPDGYQTV
                     VGERGARLSVGQRQRIAIARAFLKDAPILILDEPTSALDLRTEDRIRQTLRDLVRDRT
                     TILVSHRPSLLEIADRVLTIEDGRVRECRMPAPPSLPDLFKGCGDAPLRHGA"
     gene            2524..>2852
                     /locus_tag="PLV90_10475"
     CDS             2524..>2852
                     /locus_tag="PLV90_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97581.1"
                     /translation="MVSRAAADRWKGAVVGILAVGAVFLLGRFAILPAPVMTVAFLFG
                     GGLAAGLFTPGTVRDGAITGAACGALVALLASFGITLASLVNSEPYYPPFWMTFGFYA
                     LILTSLLL"
BASE COUNT          481 a          865 c         1015 g          491 t
ORIGIN      
        1 ccgtggcggg cgatcgtcat caggctgcgt gcggtcatct ggtttgcaag cgaccactcc
       61 tgcttcttca agggcgtcga tcccgtgatc aggctcacga agatgcccgt cccgatcgcc
      121 gcgccgcagg tgccgtggct gccgcagaac cctcccggga ccatgccggc cctcgcacgc
      181 gcctgccgga cgagcggggc tttcctcccc ggctcccccc ggtggttgca gtacgcggcg
      241 atgaggcttg ccggcacgag gaagtggtgt tccggcccgt gcatcctgac cgccgggcag
      301 cgcatcagga tacaggcgac ggcaagcgga tcctcaaggt ccgttgcgat gcagaactct
      361 tcgatgaggt cgagcgcctc aaggctgtgg caccggtcgc agacgaagtg cccggccccg
      421 caggccgccg tggtgctccg tatatccccg cagacggcgc aggggagatc gcggggttct
      481 tcgctgtaca ggatatcccg gccgcagatg aggcaccctg ctctatgctc catgtcgtag
      541 gggtgcgtcg ccgcacccct taaagagatc cgggagagag ggaggggcgg gcatgcggca
      601 ctcccgcacc cgcccatcct cgatcgtgag gacccggtcg gcgatctcca ggagcgaggg
      661 acggtgggag acgaggatgg tcgtccggtc ccggaccagg tcgcggagcg tctgccggat
      721 ccggtcctcg gttcggaggt cgagtgcgga cgtgggttcg tcgagtatga ggatgggcgc
      781 atccttcagg aacgcccggg cgatggcgat ccgctgccgc tggccgacgg agagccgggc
      841 cccgcgttcg ccgacgaccg tctggtagcc gtccgggagc cggacgatat cgtcgtggat
      901 ctgtgccttc tctgcggcct cgacgacatc cccggcggtg gcatcaggtc tgctatagcg
      961 gatgttctcc tcgatggtgg tgtgaaagag gaagaggtcc tgcgagacga tcgatatctg
     1021 ccgccgcagc caccgggggt caagcgtcga gaggtcgcgc ccgtcaagcg tgatcgtgcc
     1081 tttctcgggc gagaacgccc ggaggatcag gttgatgagc gtcgtcttcc cggcgcccgt
     1141 ccgcccgacg acggcgacca cctcgccggg ccggatgacg aactgcgcat cccggaggac
     1201 cggggtatcc tctccgtaac cgaaccagac gcgggagaac tctatacgcc cctgcacggc
     1261 gtcgggaaca aacccgccgg cgtcggggag ggcgcccggt tcgccctgca tcgcaaagag
     1321 ctcgcgcagg cgggcggccg acgtcgccgc gggctgcatc accacgggaa acgcgaagaa
     1381 ggtgctgacg gtgggggcaa acgtcatgat atagaccgcg aacgcgacga agtcccccac
     1441 ggtcatggcg ccggcgagca catcgtttcc gccgaaccat atgacggcga tcagcatgag
     1501 cgacatcgtc ccgaccctga agtactccga gaacgcggag agcatggtgc tcctgatccg
     1561 ggcgtcgacc atgttccgga gcgtcccgga gaccctgtgc acctcccggt cctctgcggc
     1621 gtgcgatttg acggtatcga taccggatat cacctccgcg aggtcccgcg agacgtaggc
     1681 ctgccgttcc cgctcccggt ggacggcggc acgaatcctt cggatgaaga cggcgttgac
     1741 caggatgtag agggggatga aggcgaggac gacgagggtc agcctgatgt ttaaggcgcc
     1801 gagtatgacg agggtgaagg cggcgtagag ggcgtttgcg acgacctggg ggaggtactg
     1861 ggagacggta tactgcagga tctggacgtc gtcggagagg cgggacatga tgtagccggt
     1921 ctgctgcgac ttgaagtagg agagcgggaa accgaggacg tgctcgaaga gggcggtctg
     1981 gaggttgaag gcgtagcctt cgcggaaccg ggcggtccag tagttccggg cgaggtctgt
     2041 tgcgccggtg aggaggccga gcacgaggag ggcggccacg agtatattga gcgatgagag
     2101 gatgccgccg gtaccggcaa ggagggcgtc gggtgccacg gcctccccga gcaggatgtt
     2161 gtcgacgaag atcttgaggc tgagcgggag gacggtcttt tcggcggaga caagcgccgt
     2221 cgtcacgaga gcgagagcgc ccgctctccg gatcggccgg gcgaaccgga ggaagaaggc
     2281 gatgtcggag aaccggagtc ctccgccggt cttcgccggc tggcgcagga tatcccgcgt
     2341 gaaggcgatg gagcgccgat agcagtctgt gatactcata gctggtccgg ttgaggagcg
     2401 ataaacccgg ggccggccgc atctccgttg cagttgcacg ccctcgtatc gtataatacc
     2461 tgcggccccg gtatgccggg ggcaaaccga aacggataac atatggcggg gtgagactct
     2521 catatggtct cgcgtgccgc cgccgatcgg tggaaggggg cggttgtggg aatcctggcg
     2581 gtcggtgcgg tctttcttct cggccggttt gctatcctcc cggccccggt catgacggtg
     2641 gcgtttctct tcggcggggg ccttgccgcc gggctcttca ccccggggac cgtcagggac
     2701 ggtgccatca ccggtgccgc gtgcggggcg ctcgtggccc ttctcgcgag ttttggtata
     2761 accctcgcga gccttgtaaa cagcgaaccc tactatcctc cgttctggat gacctttggt
     2821 ttctacgccc tgatcctcac gtcgctcctc ct
//
LOCUS       DAOSII010000217         2813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_31061_length_2813_cov_49.700508, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000217 DAOSII010000000
VERSION     DAOSII010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31061_length_2813_cov_49.700508"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..2476
                     /locus_tag="PLV90_10480"
     CDS             206..2476
                     /locus_tag="PLV90_10480"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF97582.1"
                     /translation="MIESEPDRIADGVRFPQEFLPLFGIAASALVALGVTAYCLISGI
                     FIIFPHLFYIPIILVSYYYPNRGVGATVVLSAAYLAEILFLSPVSEAEIINALVRIGV
                     FLLVAAVVSHLAGRLQVREARYRGIFETSGAGIFLFSPRTGKIEEMNQQCAAMLGYRE
                     GEAPSFDVSTIWPGYTGLTGPLEEGRIEGLDCNLVARDGTPSAVLLSASLLPDQQEGC
                     VVVTGTAELKQMENQLRRSKETLRVILDTTDVGILLTDPGRTIVEANAAAVRFFGGAG
                     REDLLGKDPYDLVAGRDREAWVACRERALHGEAHVFGECTLCRLDGTEWPAEFTITSF
                     AQGGAAPGRLVVSFRDITERRRAEREMREENRRLFITNEVMAAATASRRLDDLLPAAL
                     EKSLALLDFDLGAAHLVYPGDDRAHLRASVGDVVFPLDVSRDEPPYRYVLVDGEARFV
                     DHFQEKYPEHGNPGTRSFASVPIPGDDGPVGCITVASRARETIPEGERRILVAIGEGL
                     GSAVVKGMLHEDLEAALASANHYLEKANAATEAANLYVDILTHDINNANTVAMGYLHM
                     ALESGDKPPREFLREPLAAIYQSSDIIRNVTTIRRLEAGDVKLRPVRLESVFRGMRNY
                     FADARITCEGPDATVLADDLIDEIFSNLIGNAVKFGGPDAGITISVREEGEAVSVTVA
                     DNGPGIPDDLKPRIFERKVRGTTKKSGKGLGLSIVRMLAERYGGSVRAGDRVPGRPEE
                     GAAIMVTLRRYKAGEE"
     gene            2522..2592
                     /locus_tag="PLV90_10485"
     tRNA            2522..2592
                     /locus_tag="PLV90_10485"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2554..2556,aa:Gly,seq:ccc)
BASE COUNT          496 a          887 c          914 g          516 t
ORIGIN      
        1 agcccttgcg ggcagtgctt ccgggagcac gtcggactta ccatagccag cacatgagag
       61 tgctgcatca tattctccgg gatccgactt aagtgtatcg ggggtgcgtg atcggtgttc
      121 aggcaattct ccactcgtgt tttggtgcac cggcgcgcca tccgcttcct caaggtttat
      181 catgccccgt aatgaccatt atggcatgat cgagagcgaa ccggaccgta tcgcggatgg
      241 cgttcgtttc ccacaggagt ttcttcccct attcggtatc gccgcgtcag cgctggttgc
      301 gctcggcgtc accgcgtact gcctcatctc tggaatattc atcatcttcc cccatctctt
      361 ctacatcccg atcatccttg tctcctacta ctacccgaac cggggcgtcg gggccaccgt
      421 ggtgctctcc gccgcgtatc tcgcggagat cctcttcctc tccccggtca gcgaggcgga
      481 gatcataaac gccctggtcc gcatcggcgt gttccttctc gttgccgccg tcgtgtcgca
      541 cctcgccggc cgcctgcagg tgcgggaggc ccggtaccgg gggatcttcg agacgtcggg
      601 cgcgggcatc ttcctcttct cgccccggac cggaaagatc gaggagatga accagcagtg
      661 cgccgcgatg ctcgggtacc gggaaggcga ggctccctcc tttgatgtat ccacgatctg
      721 gcccgggtat accggactca ccggacccct tgaggagggc cggatcgaag gcctggactg
      781 caacctcgtc gcccgggacg ggacgccctc cgcggttctc ctctcggcaa gcctcctccc
      841 cgaccagcag gagggctgcg tggtggtcac cggaaccgct gaactgaagc agatggagaa
      901 tcagctccgc cgttcaaagg agacgctccg ggtcatcctc gacaccaccg atgtcgggat
      961 cctcctgacc gacccgggca ggacgatcgt ggaggcgaat gcggccgcgg tccggttctt
     1021 cggcggagcc ggacgggagg atctgctcgg gaaggaccca tacgatctgg ttgccgggag
     1081 ggaccgggag gcctgggtgg cctgccggga gcgggcgctg cacggggagg cgcatgtctt
     1141 tggggagtgc acgctctgca ggctcgacgg caccgagtgg cccgcggagt tcacgatcac
     1201 ctccttcgca cagggcggag ccgccccggg acgtctggtc gtctcgttcc gggacatcac
     1261 cgaacggcgc cgggcggaga gggagatgcg ggaggagaac cggcgtctct tcatcacgaa
     1321 cgaggtcatg gccgcggcaa ctgcgtcccg ccgccttgac gacctcctcc cggccgcgct
     1381 cgaaaaatct ctggcgctgc tggacttcga cctgggtgcg gcgcacctgg tataccccgg
     1441 ggacgatcgt gcgcacctgc gcgcctcggt gggcgatgtg gtcttcccgc tcgatgtgag
     1501 ccgggatgaa ccgccctacc ggtacgtgct cgtcgacggg gaggcccgat tcgtcgacca
     1561 tttccaggag aagtatccgg agcatggcaa ccccggcacc cgatcgtttg catcggttcc
     1621 cataccgggc gacgacgggc cggtggggtg catcacggtc gcgagcagag ctcgcgagac
     1681 gatcccggag ggcgagcgcc ggatcctcgt ggcgataggc gaagggcttg ggagtgccgt
     1741 cgtgaagggg atgctccacg aggacctgga ggcggcgctc gcgtccgcca accactatct
     1801 tgagaaggcg aacgccgcaa ccgaggcagc caacctctac gtcgatatcc tcacgcacga
     1861 catcaacaac gcgaataccg tagccatggg ctacctgcac atggccctcg agtcgggcga
     1921 taaaccaccc cgtgagttcc tccgggagcc gcttgcagcc atctaccaga gcagtgacat
     1981 catcaggaac gtcacgacga tccggagact ggaggccggt gatgttaaac ttcggccggt
     2041 acggcttgaa tctgtgttcc gggggatgcg caactacttc gccgatgccc gcatcacctg
     2101 tgaggggccg gatgcaacgg ttctcgcaga cgatctcatc gacgagatct tctcaaacct
     2161 gatcggcaac gccgtcaagt tcggggggcc ggatgccggg atcaccatca gcgtccggga
     2221 ggagggggag gcggtatcgg tgacggtcgc cgataacggc cccggtatcc ctgacgacct
     2281 caaaccgcgg atattcgagc gcaaggtgcg cgggacgacg aagaagagcg gaaaagggct
     2341 cggcctcagc atcgtcagga tgcttgcgga acgctacggg ggaagcgtcc gggcgggaga
     2401 ccgcgtcccg ggccggccgg aggagggggc ggccataatg gtgaccctgc ggcgttataa
     2461 ggccggggag gagtaaaccg aatgtttatc acctccccgg gcaacatgta atggtaccgg
     2521 cgcgatagta gtctagcggt aggacagggg cttcccaagc ctctagcccg ggttcgattc
     2581 ccggctatcg catcctgaac tcgggtttga atctcctttt cttgcatatt cattcgatag
     2641 agcacccctt gggaatcaaa atagatcggc atctcccatg tcctctccgc cgcatcgcgg
     2701 cgctcgggcg atgggtggct gcagatcccg tccgcaggta tcctccgggc accgggcctt
     2761 ccggcacgcc ccgcctgggg tgagcctgca cgacctgacg ggctttggaa gag
//
LOCUS       DAOSII010000218         2760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_31823_length_2760_cov_43.786691, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000218 DAOSII010000000
VERSION     DAOSII010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31823_length_2760_cov_43.786691"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="PLV90_10490"
     CDS             <1..1155
                     /locus_tag="PLV90_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97583.1"
                     /translation="DFVDRERETAFILNTIKNDHVMLVAPRRFGKTSIMRKIERDLAS
                     RGQSCVFLEVESVNSPGEFITDLVTALVECEGASPGARISSALGKAFALVQEKIDEIE
                     TPGFRAKLRGRLRAEFSDDWHEKAKRVDEIFAGLTGPICIILDEFPVAIQNMEEDDAR
                     KFLQWFRRLRQVSPGARFIVGGSVSIDHVVYHIGGTPVINDFRRVTIGGFSEGVALSV
                     VERVFQGEGWEYSPEIGRAVLDCVGEPCIPYFLMIVLSGLKEEADISGGVPSPALVEK
                     VYNRRILGSEGRHYFEHYSRRLQTTYSGQEAKAARAILSRVSLAESLPAGIAYEIFRQ
                     STASESEDAFRALLAQLNHDFYVTSETPGELRFYSKMLRDWWRIYHAGAW"
     gene            1139..>2760
                     /locus_tag="PLV90_10495"
     CDS             1139..>2760
                     /locus_tag="PLV90_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOF97584.1"
                     /translation="MPAPGRCYRYFPAALAGETLRHLLVGRQEIFESLFDEADRASAS
                     RTPRFFLLVGDRGAGKSHLMSLLYRRIRDELSGRMIPVDLAGEEYSIFRASDFFLRVL
                     AGMGVETADVAALGEDSMVREAAVERIAASAGERQVVVFVENIHEVFSQMERREVRAL
                     RSVFQRRDDLSVVASAPSLFSGVLDHEEPFYNFFRVFHLRELDCAEAKDLMRRVAEVD
                     GDTAFIENFGDYEPGIEALRHLVGGNPGLMVRLYEILSQCGAGKVAEAFFRLADEQTP
                     YYREVFRRLPGQRRLILDTILAAETPLSPKDVAERARLNLATVNAQLRRLEAEGYVVS
                     RPMKKRTTYEARDRLFRMWRAMRQPAGRDRVFALIGFLEAWHGRPAAVDVLYRGAPGV
                     MEGVAPPNRIAAGGREDVLAALQEAEGQTDDPGRFVHLALDLAGEELLVGNRANGLSL
                     IRAAYAHAGSLDPATARRMTIGFLKRLAGAGEVSAVGSAVREIVASGGAGWKGLLKPV
                     TDAVAIVEAKDTRLYYTRLQPEERAVVAGIVRA"
BASE COUNT          469 a          837 c          985 g          469 t
ORIGIN      
        1 gacttcgtcg accgcgagcg cgagaccgcg ttcatcctga acaccatcaa gaacgaccac
       61 gtcatgctcg tcgccccccg gcggttcggc aagacctcga tcatgcgaaa gatcgagaga
      121 gacctcgcga gcagaggtca atcgtgcgta ttccttgagg tcgagagcgt caactcgccg
      181 ggggagttca tcaccgatct cgtcacggcg ctcgtcgagt gcgagggagc gagccccgga
      241 gcccggatct cctcggcgct cgggaaggcg tttgccctgg tgcaggagaa gatcgacgag
      301 atcgagaccc caggcttcag ggcaaaactc cggggccggc tgcgagcgga gttctcggac
      361 gactggcacg agaaggcaaa gagggtggac gagatctttg cagggctcac cgggccgatc
      421 tgcatcatcc tcgacgagtt tccggttgcc atccagaaca tggaggagga cgacgcgaga
      481 aagttcctgc agtggttccg gcggctgcgg caggtctcgc ccggggcgag gttcatcgtc
      541 gggggctcgg tcagcatcga ccacgtcgtc taccacatcg gcgggacccc ggtcatcaac
      601 gacttccgga gggtcaccat cggcgggttc agcgagggtg tcgcgctctc cgtcgtcgag
      661 cgcgtctttc aaggagaagg gtgggagtat tctccggaga tcgggcgagc ggttctcgac
      721 tgcgtgggcg agccctgcat cccctacttc ctcatgatcg tcctctccgg cttaaaggaa
      781 gaggcggata tctccggagg ggtgccctcc ccggcgctgg tcgagaaggt ctacaacagg
      841 aggatcctcg gcagcgaggg gcgccactac tttgagcact actcgcgccg cctccagacg
      901 acctactccg ggcaggaggc aaaggctgcc cgtgccatct taagcagggt gagtctcgcg
      961 gagtccctgc ctgcgggtat cgcctacgag atcttccggc agagcaccgc atcagagagc
     1021 gaggatgcat ttagagcgct gctggcgcaa ctgaaccacg acttctacgt cacctcggag
     1081 acccccggcg agttgcggtt ctactcgaag atgctccggg actggtggag gatctaccat
     1141 gccggcgcct ggtaggtgtt accgctactt ccccgcggcc cttgcggggg agacgctccg
     1201 gcaccttctc gtcggccggc aggaaatctt cgaaagcctg ttcgacgaag cagatcgggc
     1261 atccgcgagc agaacgccgc ggttcttcct cctggtcggg gaccggggcg ccgggaagtc
     1321 ccacctgatg agcctgctct accggaggat acgcgacgaa ctctccggcc ggatgatccc
     1381 ggtagacctt gcaggggagg agtactcgat attcagggcg tccgacttct tcctccgggt
     1441 gctcgcgggg atgggggtcg agaccgccga cgtggccgcg ctcggcgagg attcaatggt
     1501 ccgcgaggcc gcggtcgagc ggatcgccgc atcggccggg gagaggcagg tcgtggtctt
     1561 cgtcgagaac atccacgagg tcttttccca gatggagagg cgcgaggtcc gggcgttgcg
     1621 gtcggtattc cagcggagag atgacctctc ggtcgttgcc tcggcgccgt cgctcttttc
     1681 cggggtcctc gaccatgagg aaccgtttta caacttcttc cgggtctttc acctccggga
     1741 gctcgactgt gccgaagcaa aggatctcat gcggagggtc gcggaggtcg acggcgatac
     1801 cgccttcatc gagaacttcg gggactacga gcccggcatc gaggcgctcc ggcacctcgt
     1861 cggcggcaac ccggggctga tggtccggct gtacgagatc ctctcgcagt gcggggcggg
     1921 caaggtggcg gaggccttct tcaggctggc ggacgagcag acgccctact accgggaggt
     1981 cttccggagg cttcctgggc agcggcggct catcctcgat acgatcctcg ccgcagagac
     2041 cccgctcagc ccaaaagacg tcgcagagcg cgcccggctg aacctcgcga cggtgaacgc
     2101 gcagctccgg aggctggagg ccgagggcta cgtcgtctcc cgcccgatga agaagaggac
     2161 gacctacgag gcccgggacc ggctctttcg gatgtggcgg gcgatgcggc agccggccgg
     2221 ccgtgaccgg gtgtttgccc tcatcgggtt tttagaggcc tggcatgggc ggccggcggc
     2281 cgttgacgta ttataccggg gggcgccggg cgtcatggaa ggcgttgccc cgccgaaccg
     2341 gatcgcggcc ggagggcggg aggatgtgct cgcggcgctg caggaggcgg agggacaaac
     2401 tgacgatccg ggccggttcg tgcatctcgc gctcgatctc gcgggggagg aactgctggt
     2461 cgggaaccgg gcaaacggcc tctcgctgat ccgggcggca tatgcgcacg ccggcagcct
     2521 ggacccggcg acggcacgaa ggatgacgat cgggttcctg aagcggctcg ccggggcggg
     2581 ggaggtctcc gcggtcggga gcgccgtccg cgagatcgtc gcgtccgggg gcgccgggtg
     2641 gaaggggttg ttgaaaccgg tcacggatgc cgtggcgatc gtcgaggcga aggacaccag
     2701 gctgtattac acgaggctgc agccggagga gcgggcggtc gttgccggga ttgtccgggc
//
LOCUS       DAOSII010000219         2758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_31849_length_2758_cov_49.193119, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000219 DAOSII010000000
VERSION     DAOSII010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31849_length_2758_cov_49.193119"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..1903
                     /gene="acs"
                     /locus_tag="PLV90_10500"
     CDS             14..1903
                     /gene="acs"
                     /locus_tag="PLV90_10500"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOF97585.1"
                     /translation="MTETFAVKLEEKCYLPDPRCKAAAWTPDYTRAYQEFMHDPEAFW
                     DQVAHELDWFEPWDRVLEWNYPYAKWFVNGKLNITYNCLDRHVTSDRRDKPAVIWRGE
                     GGEERRLTYDGLYREVMRCANALAGLGVGKGDRVCIYMPLVPEQIVAMLACARLGAVH
                     SVVFGGFGAGALAMRINDAEAKVVITADVGYRRGKTIPLRDIVNEALTHAPCVEKTVV
                     FRRGTPAVDLDPAREVDYADLVARAAPDCPAEVMDAEDPLFILYTSGSTGAPKGIVHT
                     CGGYMVGTYYTSRHDFDIKESDILWCTADPGWITGHSYIVYGPLAVGTTVVIAEGTPD
                     YPDPGAYWRLVQDLGVTIFYTAPTAIRMFMRHGDAWPAKYDLSTLRVLGSVGEPLNPE
                     AFEWYYHRIGGGECPVVDTWWQTETGMHVVTTMIGEAMKPGFAGRAIPGAVVDVVDRT
                     GKPVPPGTGGFLVIREPWPAMLRTVHGNDARYRTYWETIPGCYTAGDLAVKDEDGYIM
                     ILGRADDLIVVAGHNIGTAEVESALVSHDAVAEAAVIGKPDALKGNVIKAFVTLRVGA
                     NPGDALTDELARHVKKSLGPVAVPAEIEFMDRLPKTRSGKIMRRVLKARELGMDPGDI
                     STLEE"
     gene            1910..>2758
                     /locus_tag="PLV90_10505"
     CDS             1910..>2758
                     /locus_tag="PLV90_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF97586.1"
                     /translation="MTETPTIFGLPAERGRWGLIALGLLINLCLGSIYSWSVFVAPLA
                     AHFTATLGREVAAGEVLLPFSVFLAFFAIAMPLAGKYIERYGPRKVTVAGGLLTGLGW
                     LLASTATSVEALYVVYGVIGGLGVGIAYGAPVAVAARWFPDRRGLAVGLTLLGFGVSA
                     FVTANAAGALIAAYGVMTTFAIFGVVLAAVLVVAALPLRFPPAGWQPAGWSAPAPGTG
                     TAPACECDRRAMLRTGTFYGLFACYFVGCLAGLTAISIAKPVGTEVAGVDAGLATLLV
                     GFFAIFN"
BASE COUNT          445 a          926 c          935 g          452 t
ORIGIN      
        1 ggaatgagga tatatgacag agacatttgc tgtcaaactg gaggagaagt gctacctccc
       61 cgacccccgg tgcaaggcgg ccgcctggac gccggactac acccgggcgt accaggagtt
      121 catgcacgac ccggaggcct tctgggacca ggtcgcccac gaactcgact ggttcgagcc
      181 ctgggatcgg gttcttgagt ggaactaccc ctacgcgaag tggttcgtca acggaaaact
      241 caacatcacc tacaactgcc tcgaccgcca cgtcacgagc gaccgcaggg ataaaccggc
      301 ggtcatatgg cgcggcgagg gcggggaaga gcggagactc acctacgacg gcctctaccg
      361 cgaggtgatg cggtgtgcaa acgcccttgc gggcctcggc gtcgggaaag gcgaccgggt
      421 ctgcatctac atgccgctcg tccccgagca gatcgtcgcg atgcttgcct gcgcgcggct
      481 cggggccgtc cattcggtgg tcttcggcgg gttcggcgcg ggcgcgctcg cgatgcggat
      541 caacgatgcg gaggcaaagg tcgtcatcac cgcagatgtc ggctaccggc ggggaaagac
      601 catcccgctc cgcgatatcg tgaacgaggc gctcacccac gcgccgtgcg tcgaaaagac
      661 cgtcgtcttc cggcggggga caccggccgt cgacctcgat cccgcgcggg aggtcgacta
      721 cgccgatctt gtggcccggg ctgcaccgga ctgccctgcg gaggtgatgg atgcggaaga
      781 cccgctcttc atcctctaca cgagcggctc gaccggcgca ccgaagggta tcgtccatac
      841 ctgcggcggc tacatggtcg ggacctacta cacctcccgc cacgacttcg acatcaagga
      901 gagcgatatc ctctggtgca ccgccgaccc cgggtggatc accggccaca gctacatcgt
      961 ctacggcccg cttgccgtcg ggacgaccgt cgtcatcgcc gaagggacac ccgactaccc
     1021 ggacccgggc gcctactggc ggctggtcca ggacctcggg gtgacgatct tctataccgc
     1081 cccgaccgca atccgcatgt tcatgcgcca cggtgatgcg tggccggcga agtacgacct
     1141 ttcaacgctg cgcgtgctcg gctccgtcgg cgaacccctc aaccccgagg ccttcgagtg
     1201 gtactaccac cggatcggcg gcggagagtg cccggtcgtg gatacctggt ggcagaccga
     1261 gaccggcatg cacgtcgtca ccacgatgat cggcgaggcg atgaaacccg ggtttgccgg
     1321 aagagccata ccgggcgccg tcgtggatgt cgtcgaccgg acgggtaagc cggtcccgcc
     1381 cggcaccggg gggttcctgg tcatcaggga gccctggccg gccatgctcc ggacggtcca
     1441 cgggaacgat gcgcgctacc gcacctactg ggagaccatc ccgggctgct acactgcagg
     1501 agacctcgcg gtgaaggacg aggacggcta catcatgatc ctcggccggg ccgacgacct
     1561 gatcgtcgtc gccgggcaca acatcgggac cgcggaggtc gagagcgcgc tcgtctccca
     1621 cgatgcggtg gcggaggccg cggtcatcgg gaagcccgat gccctgaaag ggaacgtcat
     1681 caaggcgttc gtcaccctcc gcgtgggcgc aaatcccggc gacgccctca ccgacgaact
     1741 cgcccgccac gtcaagaaga gcctcgggcc cgttgcagtg ccggccgaga tcgagttcat
     1801 ggatcggctc ccaaagaccc ggagcggcaa gatcatgcgc cgggtcttga aagcccgcga
     1861 gctcggcatg gacccgggcg acatatcgac actcgaggag tagacgggca tgacagagac
     1921 accgacgatc tttgggctgc cggcggagag ggggaggtgg gggctcatcg ccctcggcct
     1981 cctcatcaac ctctgcctcg ggagcatata ctcctggagc gtctttgtcg cgccgctcgc
     2041 ggcgcatttc accgcgacac tcggccggga ggtggcggcg ggggaggtcc tcctcccgtt
     2101 ctcggtcttc ctcgccttct tcgctatagc gatgccgctc gccgggaagt atatcgaacg
     2161 ctacgggccg cggaaggtga cggtcgccgg cgggctcctc accggcctcg ggtggctcct
     2221 cgcctcgacg gcgacgtcgg tcgaggccct ctacgtcgtc tacggggtga taggggggct
     2281 cggtgtcggc atcgcctacg gggcgccggt cgccgtcgcg gcccggtggt tcccggaccg
     2341 gcgggggctc gcggtcgggc tcaccctgct cgggttcggg gtttcggcgt tcgtcaccgc
     2401 aaacgccgca ggagccctga tcgctgccta cggcgtgatg acgacgtttg cgatattcgg
     2461 cgtcgttctc gccgccgtgc tggtggttgc agccctgccg ctccggttcc cgccggcggg
     2521 ctggcaaccg gcggggtggt cggcgcctgc gcccggcacg ggcacggccc cggcctgcga
     2581 gtgcgaccgc cgcgctatgc tccggacggg gaccttctac ggcctcttcg cctgctactt
     2641 cgtcggctgc ctcgcggggc tcaccgcgat atcgatagca aagccggtcg ggaccgaggt
     2701 cgccggggtg gatgcggggc ttgcgacgct cctcgtcggg ttcttcgcca tattcaac
//
LOCUS       DAOSII010000220         2730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_32294_length_2730_cov_28.405981, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000220 DAOSII010000000
VERSION     DAOSII010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32294_length_2730_cov_28.405981"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(287..1498)
                     /locus_tag="PLV90_10510"
     CDS             complement(287..1498)
                     /locus_tag="PLV90_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97587.1"
                     /translation="MKDRIRDLLLLGMVIALLIPAATAADIYITVTADDPTEYRGLRV
                     ATVDALDPLETDASLVTLEGDNLTVIPCPTFGTITRIFDPASGTYRGGRVTITENKSF
                     DGSTLPDDYRIVTGRTIPNFTMHVPNRSGNVLMVRSLGSPEEGIDFARFFEAAEELGY
                     LFTDDAIIYSNTTGISAYGNIDTQDYDRNDLNRTLKRNEVDLANFSADSDMVRSMMEA
                     WPYTRPEAGEYLLTAVQYDSENETLHVLAAMPVLILDGNPTVAWNDDRQGAGATVSFG
                     EGVDRTAYILLESNMTYDLTVRVDTREFVSRPIPTSVTDLVSLLQTAADEASPVAYTL
                     TRSDDDAGSGIVIAPGHGLSGRADGPEVEIGAEALATLNPGTYSLYALGMEGGEIVAV
                     DYREVEVRAAS"
     gene            2215..>2730
                     /locus_tag="PLV90_10515"
     CDS             2215..>2730
                     /locus_tag="PLV90_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOF97588.1"
                     /translation="MPQDIPGAESLLNFDLVARTPGVAAMSILLAILLIGPVAGNDGN
                     GGRVSSLIDTPTTPRSGVRLSQPAGAVRTLVAMPAYNEEAAIAKTVLGARQHADAVLV
                     VDDGSKDETARIAGALGAIVVRHEANRGYGGALQTIFGTARGMGAEELVIIDADGQHD
                     PGEIPGLLAELR"
BASE COUNT          469 a          817 c          871 g          573 t
ORIGIN      
        1 agaggacgaa gaagacctca aggtcgagcg tgcgggagag gaccatgaag aaggcaatgg
       61 tgatgagcca ggctgcgagt acggtcgcag cgtaacactt cttcgcgccg gtcaacttct
      121 ccccccggcg ctcgataacc cggccccgtc tccccggagc aggggccggg atctcagcgg
      181 ttccgccgct atcggcaacc cctcctgccg gcaggagagg ttgtccggca tgctgcacgc
      241 ccttctggta ggggtgggct ttcccgatcc ccggcgggtg ccggtatcat gaggctgccc
      301 ggacctcgac ctcccggtaa tcgaccgcaa cgatctcccc accctccatg cccagggcgt
      361 agagggagta ggtgccgggg ttgagggttg caagggcctc ggccccgatc tccacctcgg
      421 gcccgtctgc acgcccggag agcccatgac ccggggcgat gacgatgccg gaacccgcgt
      481 cgtcatcgct ccgggtcagg gtgtaggcaa ccgggctggc ctcatctgca gccgtctgca
      541 ggagggagac cagatcggtc acggaggtcg ggatgggccg gctcacgaac tccctggtat
      601 cgacccttac cgtgaggtcg taggtcatgt tgctctcaag caggatgtac gccgtccggt
      661 ccacgccttc cccgaaggag acggttgcac cggcaccctg gcgatcatcg ttccaggcga
      721 cggtggggtt gccgtcgagt atgaggaccg gcatggcggc aaggacgtgc aacgtctcgt
      781 tttcgctgtc gtactgcacc gcggtgagga gatactcgcc ggcctccggc cgcgtgtagg
      841 gccaggcttc catcatactc cgtaccatat cgctgtctgc actgaagttg gcgagatcga
      901 cctcgttcct tttcagcgtg cggttcaggt cgtttctgtc gtagtcctgc gtatcgatgt
      961 tgccataggc cgatatgccc gtcgtgttgc tgtagatgat ggcatcgtcg gtgaagaggt
     1021 agcccagctc ttccgcggcc tcaaagaatc tggcgaagtc gatgccttcc tcagggctcc
     1081 ccagggatct gaccatgagg acgttgccgg accggttcgg gacgtgcatc gtgaagttcg
     1141 ggatcgtccg gccggtaacg atccggtaat cgtccgggag ggtgctcccg tcgaaggact
     1201 tgttctccgt gatggtcacc ctcccaccgc ggtaggtgcc gcttgcggga tcgaagatcc
     1261 gggttatcgt cccgaaggtc gggcacggga tgacggtgag gttgtcaccc tcaagggtca
     1321 cgagggaggc atcggtctcc agggggtcga gggcgtcgac ggtcgcgacg cgaagccccc
     1381 tgtactccgt cggatcatcg gccgtcaccg tgatgtagat atctgcggcg gttgccgcag
     1441 ggatcaggag ggcgatgacc atacccagca acaggaggtc tcgtatacga tccttcatgg
     1501 gtggcccatt ggtaaccttt accataaact ttttcatcgc gatgaatggc ccggctctgt
     1561 atggcaggtt ctttcacgga gcacctccgc gaccgggatc ccttgcctgg agatgcggcc
     1621 ccggctatcc cggatgatcc ccgcgggatg tggagtgtaa ctccggggca ctggagacct
     1681 gtttccatga agttttcccg gccggcccat cgatccccgc tgtcgaaata aattctcctg
     1741 ataaggcagt atttttgaat agtattcgaa acgtcatgcc cgcgaggtac ggccataggc
     1801 aagctttatt atattggata ttctcgatct ctcagaagct tctctccgcc tccttaattg
     1861 tcatcccatc tattcagggg agtttatcgc cagaacatga tatttggtgg cgattctcct
     1921 tatttgccct gtccccgctt caggcggcca tctggaaagt tatatatacc atatccttct
     1981 ctatgacccc aggccctggt gccaggaacc cacctccttt tcagtcgtgc cggtctggaa
     2041 aggggttacc cgcctccacc cggcgggtat tgacctggcc ggatacgggt cttgcgttag
     2101 gggtgtgtgc ctgatgtccg ggcgggtgcc gtattgtacg tggtgtgtga ccctgccggg
     2161 gtccggtgag ggtaagaaca gcgctcaatc tatcgaagtt acgggtgttc ctccttgccg
     2221 caggatatcc cgggagcaga aagcctgctc aacttcgatc ttgttgctcg cactccgggg
     2281 gtggccgcca tgagtatcct tcttgccatc ctcctgatcg ggccggttgc cggcaacgac
     2341 ggtaatgggg gaagagtctc ttctcttatc gacactccga caaccccccg ttctggggtg
     2401 cggctatcac agccggccgg tgccgtccgg accctggtcg cgatgccggc ctacaacgag
     2461 gaggctgcaa tcgcaaagac ggttctcggc gcacggcagc acgcggatgc ggtcctggtc
     2521 gtcgacgacg gctcgaagga cgagaccgcc cggattgccg gggcgctcgg ggcgatcgtc
     2581 gtccggcacg aggcgaaccg gggctacggc ggggcactgc agacgatctt cggcactgcc
     2641 cgggggatgg gtgcggagga gctcgtcatc atcgatgccg atggtcagca cgacccgggg
     2701 gagatcccgg ggctgcttgc cgagctacgg
//
LOCUS       DAOSII010000221         2729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_32309_length_2729_cov_38.428197, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000221 DAOSII010000000
VERSION     DAOSII010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32309_length_2729_cov_38.428197"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PLV90_10520"
     CDS             complement(<1..389)
                     /locus_tag="PLV90_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOF97589.1"
                     /translation="METCYDILGVAPDATPDEIRAAYRRLARQYHPDVNPDPDASERF
                     IAIHQAYEVLIDPEARARYDLSLRHQAAGAGGQAAWAPGFTWRAQAPGSEAGRPGTIA
                     ALAIWGMMVLLMLVLSLLLRLVIGGRQR"
     gene            complement(398..796)
                     /locus_tag="PLV90_10525"
     CDS             complement(398..796)
                     /locus_tag="PLV90_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HOF97590.1"
                     /translation="MQGRGISRGIGSGDLLVSPEPISFLSGVDPETGIVIERGHPLEG
                     QSIAGRVLAFPYGKGSTVGSYVIYALKRNDRAPVAIINTEAEPIIAVGAIIAGIPMVD
                     RLPPEFSCLAPGTRVTVNGDTGEVSYVLDT"
     gene            838..2010
                     /locus_tag="PLV90_10530"
     CDS             838..2010
                     /locus_tag="PLV90_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-activating nucleotidase"
                     /protein_id="HOF97591.1"
                     /translation="MSNMDETVGSSPGTEHDMLNLQIQDLKAQILDYRLKNELLEKEV
                     MQLRKENAQLKRVPLFVAAVVDVLENGEVYLRQQGNNQEYVTTVSEKLHRILKPGMKV
                     AVNNTLSVVRTIGNTYDSRVRVMELDEQPSVTFEQVGGLKEEIEEVREAVEYPLTKPK
                     IYERVGVEPPKGILLYGPPGTGKTLIAKAVARQSRARFIRMSGSELVHKYIGEGAQLV
                     RELFVLARERAPAIVFIDEIDAIGSMRTNDGTSGSAEVQRTLMQLLAEMDGFGNRGNV
                     RIMAATNRIDMLDPALLRPGRFDRIIRIPLPDAGARLEILKIHTAKMSLGKDVDLAGL
                     AELAEQTTGAELQAICREAGMMAVRRDANAVDHEDFLVALRKVKREVAAPDTRMYI"
     gene            2188..2631
                     /locus_tag="PLV90_10535"
     CDS             2188..2631
                     /locus_tag="PLV90_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5804 family protein"
                     /protein_id="HOF97592.1"
                     /translation="MNVLLIQPEGVDLYTTLLKSETSRGALRFYQPDRLEYGIRVRTA
                     SLGSALALASELRWYVQRYVEEVLFEPEKDIFCTSGLARQIYERDVRPGSPWRYRCLY
                     RISGDHLTDTIWMDEGTAPEAYAGRMEPGDTVLEVWCTEEEYPGR"
BASE COUNT          520 a          870 c          880 g          459 t
ORIGIN      
        1 cgctgccgac cgccgataac caagcgtaag aggagcgaga gtacgagcat gaggaggacc
       61 atcatgcccc agatggccag ggctgctatg gtgccgggtc gcccggcctc tgatccgggc
      121 gcctgtgctc tccaggtgaa ccccggcgcc catgccgcct gccctcccgc gccggccgcc
      181 tggtgcctga gggagaggtc gtagcgggcc cgggcctccg gatcgatcag cacctcgtat
      241 gcctggtgaa tagcgatgaa ccgctcgctc gcatcggggt caggattgac gtccgggtgg
      301 tattgcctcg cgagtctccg gtaggccgcc cggatctcat cgggtgtcgc gtcgggtgcg
      361 acccccagga tatcgtagca ggtctccaca gggggcatca cgtatcgagg acgtatgata
      421 cctcgcccgt atccccgttc accgtcaccc gggtgccggg ggcgaggcag gagaactccg
      481 gcgggaggcg gtcgaccatc gggatccccg cgatgatcgc gccgaccgcg atgatcggtt
      541 ccgcctcggt gttgatgatc gcgaccgggg cccggtcatt ccgtttgagc gcgtagatga
      601 cgtaagatcc gaccgtcgat cccttcccgt acgggaatgc gagcacgcgc ccggcgatgg
      661 attggccctc aagcgggtgt ccgcgttcga tgacgatacc cgtctcgggg tcgacgcccg
      721 agaggaacga tatgggttcg ggcgaaacaa ggaggtctcc gctcccgata cccctggata
      781 tccctctgcc ctgcatggtc actgccacca ctataatgag gttggagatc caactatatg
      841 agtaacatgg acgaaaccgt tggcagcagt cctggcaccg agcacgacat gctcaacctc
      901 cagatccagg acctgaaggc gcagatcctg gattataggt taaaaaacga gttgcttgag
      961 aaggaggtca tgcaactgcg caaagagaac gctcagctga agcgggtgcc gctcttcgtt
     1021 gccgctgtgg tcgacgtgct ggagaacggc gaggtatacc tccggcagca gggcaacaac
     1081 caggagtacg tcaccacggt cagcgagaag ctccaccgca tcctcaagcc cgggatgaag
     1141 gtggcggtga acaacacgct ctccgtcgtc aggacgatcg gcaacaccta cgactcgagg
     1201 gtccgggtca tggaactcga cgagcagccg agcgtcacgt tcgagcaggt cggcggcctg
     1261 aaggaggaga tcgaggaggt cagggaagcc gtcgagtacc cgctcacgaa acccaagatc
     1321 tacgaacgcg tgggcgtcga acccccgaaa ggcatcctcc tctacggccc gcccgggacc
     1381 gggaagacgc tgattgcaaa ggctgttgcc cgccaatcgc gcgcccggtt catcaggatg
     1441 tccgggagcg aactcgtcca caaatacatc ggggaggggg cgcaactcgt gcgtgaactc
     1501 ttcgtcctcg cccgggaacg cgccccggcg atcgtcttca tcgacgagat cgacgcgatc
     1561 gggagcatgc gcaccaacga cgggacgtcc ggcagcgccg aggtccagcg gacgctgatg
     1621 cagctcctgg cggagatgga tggttttggg aaccgcggca acgtccggat catggcggcg
     1681 acgaaccgga tcgatatgct ggacccggcc ctccttcgcc cgggccggtt cgaccgcatc
     1741 atacggattc cccttcccga tgccggggca cgcctcgaga tcctgaagat ccataccgca
     1801 aagatgtccc ttggaaagga cgtggacctc gccgggctcg cggagcttgc cgaacagacc
     1861 accggtgcgg aactgcaggc gatctgccgc gaggccggca tgatggcggt ccggcgggat
     1921 gccaatgccg tcgaccacga ggatttcctc gtggcgctca ggaaggtgaa gcgcgaggtg
     1981 gcggctcccg acacccgcat gtatatctga cggggactcc tccacccccg gtttttccac
     2041 gagaaaccca tcccggggag acggcgtcct gtccttcgcg ctcattcctg cgcccggcac
     2101 ccggatcttc acctctgcag ccttttgctc ttccccatat gcacccctga ggaaccctta
     2161 tcgacctccc gcgcccacac ctatgcgatg aacgtcctcc tcattcagcc cgagggggtt
     2221 gacctctaca ccaccctgct caaatccgag accagcaggg gggcccttcg tttttatcag
     2281 cccgacaggc tggagtacgg catacgtgtc cggacggcgt cgctcggaag tgcgcttgcc
     2341 ctcgcctccg agctccggtg gtatgtccag cgctacgtcg aggaggttct cttcgagccg
     2401 gagaaagaca tcttctgcac atccgggctt gcccggcaga tctacgagcg ggacgtgcgg
     2461 ccgggatcgc cgtggcggta ccgctgcctc taccgcatct ccggggacca cctgaccgac
     2521 accatatgga tggacgaggg cacggcgccg gaggcgtatg ccggccggat ggagcccggg
     2581 gatacagtgc tcgaggtctg gtgcactgaa gaggagtacc cgggcaggta gaggatcggg
     2641 tgcgcccccg ttcacaacgc ggcctggagg agggtcgcgg ccagcgtgat cacgaggatc
     2701 gccgggacgc agtagcggat gagcaccat
//
LOCUS       DAOSII010000222         2718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_32478_length_2718_cov_54.206910, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000222 DAOSII010000000
VERSION     DAOSII010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32478_length_2718_cov_54.206910"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PLV90_10540"
     CDS             <1..645
                     /locus_tag="PLV90_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HOF97593.1"
                     /translation="LCETVKRTAFKVTRAGQLVAQEASERLGIPFGIVDLSLAPTPSV
                     GDSVAGILEEMGLESAGAPGTTAALALLNDQVKKGGIMASSFVGGLSGAFIPVSEDQG
                     MIDAVNRGALTIEKLEAMTCVCSVGLDMIAIPGDTPASTISGIIADEAAIGMINQKTT
                     AVRLIPVIGKDVGDTVEFGGLLGHAPVQQVNRFGCADFINRGGRIPAPIHSFKN"
     gene            731..1135
                     /locus_tag="PLV90_10545"
     CDS             731..1135
                     /locus_tag="PLV90_10545"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOF97594.1"
                     /translation="MVAREDSARFFAADAFARHNGIECVEVAPGRAVVKMEVRGHHLN
                     SHGTVHGGALFTLADTAFALASNSHGVPAAAINAQISYLTAAASGPLYAEAEEYAVNP
                     KLASYTVRITNDAAEKIAIFQGMVYRKTPRRD"
     gene            1176..1664
                     /locus_tag="PLV90_10550"
     CDS             1176..1664
                     /locus_tag="PLV90_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HOF97595.1"
                     /translation="MTSEELREYLAFLLWHYRVVDAFWFIYIAEEFGQPTAEAVNERV
                     WGRVAPMAAKDLVARFGIREKGLRGFVKVLKLFPWAIIVNYDIEEREDEVIITVSSCP
                     SQEARLRRGLGEYVCREMHRAEFTGFARQIDERIAVECLFAPPDPHPEGIFCKWRFYL
                     RE"
     gene            2111..>2718
                     /locus_tag="PLV90_10555"
     CDS             2111..>2718
                     /locus_tag="PLV90_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOF97596.1"
                     /translation="MHRIIDWNELWKALHVSSPERAEKDRNPAAHWDKRAAAYRRVTR
                     DERSATEQELAILGVQPGETVLDMGAGTGRLAVPVARTAAHVTALDPSEGMLAILRER
                     MAAEGLTNYSCLRKRWEDATIGRDVEPHDIVVAAFSLGFYDLAAALEKLDAAARRAVY
                     IFWHAGEWRNPGEMALYRTVFGDEAAGEKGYPDYIFPVNILH"
BASE COUNT          506 a          876 c          878 g          458 t
ORIGIN      
        1 ctctgcgaga cggtcaagag gacggccttc aaggtcaccc gcgcagggca gctcgtcgcc
       61 caggaggcgt cggagagact cgggatcccg ttcgggatcg tggatctctc tcttgccccc
      121 accccctccg tcggggacag cgtcgccggt atcctcgagg agatggggct tgagtcggcc
      181 ggtgcgccgg ggacgacggc cgctctcgct ctcttgaacg accaggtgaa gaagggcggg
      241 atcatggcga gctcgttcgt cggcgggctc tcgggggcgt tcatcccggt cagcgaggac
      301 caggggatga tcgatgcggt gaaccgcggc gccctcacga tcgagaagct cgaggcgatg
      361 acctgtgtct gctcggtcgg cctcgacatg atcgcgatac cgggcgacac acccgcttcg
      421 acgatatccg gaatcatcgc ggatgaagcc gccatcggta tgatcaacca gaagacgacc
      481 gccgtccggc tgatcccggt gatcgggaag gatgtcggcg ataccgtcga gttcggcggg
      541 ttgctcgggc atgcgccggt ccagcaggtg aaccggttcg gttgcgcgga cttcatcaac
      601 cgcggcgggc ggatccccgc accgattcac agtttcaaga actgagccgc ccgggaagac
      661 ccggctcctt tttcgcgact ctcgcaccgg actgataggc ttattacggt caccggagag
      721 attaccccat atggtcgcac gagaagactc agcccggttc tttgccgccg atgcctttgc
      781 ccgacacaac gggatcgagt gcgtcgaggt cgcgcccggc agggcggtgg tgaagatgga
      841 ggtccggggt catcacttaa acagccacgg aaccgtccac ggcggagcgc tcttcaccct
      901 cgccgatacc gcctttgctc tcgcatcgaa ctcccacggg gtcccggcgg cagcgataaa
      961 cgcccagata tcctacctca cggccgcagc gtccggccct ctctatgccg aggcggagga
     1021 gtacgccgtg aatccgaaac tggcctccta caccgtcagg ataacgaacg atgccgcgga
     1081 gaagatcgcc atcttccagg ggatggtcta ccgcaagaca ccccggcgtg actgataggg
     1141 tggtgattac gacgaacagc gatatcttcg agaggatgac ctcagaagaa ctccgggagt
     1201 acctcgcgtt cctcctctgg cactaccggg tggtggatgc gttctggttc atctacatcg
     1261 ccgaggagtt cggccagcct accgcggagg cggtcaacga gcgtgtctgg ggccgggtcg
     1321 ccccgatggc tgcaaaagac ctcgttgcca ggttcggtat ccgggagaag gggcttcggg
     1381 gtttcgtaaa ggtgctgaaa ctcttcccct gggctatcat cgtgaactac gatatcgagg
     1441 agcgggaaga cgaggtcatc atcaccgtct cctcgtgccc gagccaggaa gcacggttgc
     1501 ggcgcggcct cggtgaatac gtctgccgcg agatgcatcg ggcggagttc acggggtttg
     1561 cacggcagat cgatgagcgg attgccgtcg agtgcctttt tgctccgccg gacccgcacc
     1621 cggagggcat cttctgcaag tggcggttct acctgaggga ataggcgctc cacgcacaac
     1681 cgatatcccg gatatgtcct tctcttccgc gggggaaccg tgacgggcac cgcacaaaag
     1741 tcccccccga aaacagacgc tgcctttcgg gggcacgacg gcacccctgc ccggacagcc
     1801 ggggatgcag atcccctccc cctcgacgat ggggtagatg aacgcgctcg tctcgcggca
     1861 gaactccgcc gcgtgccccg gccccgcctc caggcaggcg gtcccggccg ggtcgccctc
     1921 tcccgtacgg acccgcatcc ggacaccgcg ctgttcacga tcgaccgcct catcgggcgc
     1981 gacgaccgcg tgagcggcag ggatcactcg agggagtcac gggcagcgcg gcctgccgtg
     2041 agtcgttctg ccggagatac gatccggtga agaacagaca agatatacga ttacacggaa
     2101 aggaacatcc atgcacagga tcatcgactg gaacgagctc tggaaagcgc tccacgtgag
     2161 ttcgcctgaa cgtgccgaga aagaccgcaa tcccgccgct cactgggaca aacgcgccgc
     2221 cgcctaccgc cgggtcaccc gcgatgaacg atcggcaacc gagcaggaac tcgcgatcct
     2281 tggggtacaa cccggcgaga ccgtgctcga catgggtgcc gggacgggga ggctcgccgt
     2341 gccggttgcc cggaccgccg cccacgtcac ggcccttgac ccttccgagg gcatgctcgc
     2401 catcctccgg gagcggatgg ccgcagaggg gctcacgaac tactcctgtc tccggaaacg
     2461 ctgggaggat gcgacgatcg ggagagacgt cgagccccac gacatcgtcg tcgcggcgtt
     2521 ctcgctcggg ttctacgatc ttgccgccgc cctcgagaaa ctcgacgccg cggcccgccg
     2581 ggcggtctac atcttctggc atgcgggcga gtggcggaac cccggggaga tggcgcttta
     2641 ccgtacggtc ttcggggatg aagcggccgg agagaagggc tacccggact acatcttccc
     2701 ggtcaacatc ctccacga
//
LOCUS       DAOSII010000223         2695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_32816_length_2695_cov_44.898106, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000223 DAOSII010000000
VERSION     DAOSII010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32816_length_2695_cov_44.898106"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PLV90_10560"
     CDS             <1..500
                     /locus_tag="PLV90_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2240 family protein"
                     /protein_id="HOF97597.1"
                     /translation="GPAENPQGCLRVSVQIAVAAPFKHMRKERLQRSEFVFYIAIDRK
                     WMNKEQANQLLERAKSEGLIEIEGGAIRPLFDLAAISIPLGFRPTSDILAAEDPLGDL
                     IGRIAAATGRPVQDIVAEVHRVVTDHFDGNLRVEAAAVILARKYGVAFEDKLSALQRS
                     VLKAR"
     gene            complement(531..1073)
                     /locus_tag="PLV90_10565"
     CDS             complement(531..1073)
                     /locus_tag="PLV90_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOF97598.1"
                     /translation="MSMDTASPILRPVGIIRNAVQEPFLVAGRDGISQREGADASRTH
                     IRSTKEAISEIILDESRIALLDGIEGYSHVTVLYWGHKVPEESRSVDRVHPMGRADIP
                     KTGIFSTCSPARPNPVLATVVRLHARRGNVLEVTGLDAVDGSPLIDIKPYVPSHYPQA
                     DVRVPAWMEELMKEIDERRQ"
     gene            1169..2167
                     /locus_tag="PLV90_10570"
     CDS             1169..2167
                     /locus_tag="PLV90_10570"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOF97599.1"
                     /translation="MADLASCRLCEWRCGVDRLQGERGVCRTGRAEVAATMCSGTLAS
                     YIVTLLGCCYRCLHCNAYRISQYPDPGWHYAGHVPAAVLVADARERIAAYRGPRIRTI
                     GFTGGDPIIHLPYIEEVAAEVRRQGLDLGIGISTGGFATAATMARIADLCRVITLEIK
                     AWSDPVHRALTGAPVAPVLRNAEYLAREGRDRIRVFRTVVIPGMTDSEVGAIATFIAS
                     LDPGIPYRLIGFRPNNVLYYHPGPSAEEMDRLCAISREAGLDDVAWSGYYPETVPDGI
                     RALAARLADAYGGDSAAALTAAYAAACGCPTHPRDCGACPLRGNCPATLREPWRGR"
     gene            complement(2226..2624)
                     /locus_tag="PLV90_10575"
     CDS             complement(2226..2624)
                     /locus_tag="PLV90_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6e"
                     /protein_id="HOF97600.1"
                     /translation="MADFKIILSDPGTGRSYKIDATGPAAGALIGKRIGDEIDGSVLG
                     LPGYTIRLTGGTDKTGIPARRDLPGPARRRLLLSRGVGFHPVMEGERRRKSVRGREIS
                     ADFVQINAAVAQSGAKPLAEYFSQPAAATE"
BASE COUNT          475 a          852 c          867 g          501 t
ORIGIN      
        1 atggacctgc ggagaatcct caagggtgcc tgcgagtgag cgtgcagatt gcggtcgccg
       61 caccgttcaa acatatgcgc aaggagcgat tgcagaggag cgagttcgtc ttctatatcg
      121 ccatcgaccg gaagtggatg aacaaagagc aggcaaacca gcttctagag cgggcgaagt
      181 cggaagggct catcgagata gagggaggcg ccatccgacc gctcttcgat ctcgcggcga
      241 tctcgatacc gctcgggttc agacccacat ccgatatact cgccgcggag gaccccctgg
      301 gagatctcat cggtcgcatc gccgctgcga ccgggagacc ggtgcaggat atcgttgctg
      361 aggttcaccg ggtcgtcacc gaccacttcg acggcaacct ccgggtggag gcggcggcgg
      421 tcatcctcgc aagaaaatac ggggtggcgt tcgaggataa actgtccgcc ctccagcgat
      481 cagtcctgaa agcgcggtaa gacttcattc gaccgcccct cttcgcaggt ttactgccgc
      541 cgctcgtcta tctctttcat cagctcctcc atccatgccg gaacccgcac atcggcctgt
      601 gggtaatggg acgggacgta gggtttgata tcgatgagcg ggctgccgtc gacggcatca
      661 agacccgtaa cctcgagtac gttcccgcgc cgcgcatgca gccggacgac ggtcgcgagc
      721 acggggttcg ggcgggcagg actgcaggtc gagaagatcc ccgtcttcgg tatgtccgcc
      781 ctgcccatcg ggtgcacgcg gtcgacggac cggctctcct ccggcacttt gtgcccccag
      841 tagagcaccg tgacgtggga gtagccctcg ataccgtcaa gcagcgcgat acggctctca
      901 tcgaggataa tctccgatat cgcctctttc gtgctccgga tatgcgtcct gctcgcgtcg
      961 gccccttccc gctgtgagat gccgtccctg ccggcgacga ggaacggttc ctgcacggcg
     1021 ttacggatta tccccacggg ccgcaggatc ggtgatgcgg tatccatgct catactctgg
     1081 catccgctcg cgaatagttg caccggtggc ggcatggctc ctgccggatt cccagagatt
     1141 atggtgcatg cagacaccag agaagagtat ggcagatctt gcgtcctgtc gtctctgcga
     1201 gtggcggtgc ggagtcgacc gcctgcaagg ggagcggggg gtatgccgca ccggccgcgc
     1261 agaggtggcg gcgacgatgt gttcgggcac tctcgcaagt tacatcgtca ccctgctcgg
     1321 gtgctgttac cgctgcctcc actgcaacgc ctaccggata tcacagtacc ccgatcccgg
     1381 ctggcactac gccggccacg tgcctgcggc ggtgctggtg gccgatgccc gggagcggat
     1441 tgccgcgtac cgggggcccc gcatacggac gatcgggttt acgggcgggg atccgatcat
     1501 ccacctcccc tacatcgagg aggtcgcggc cgaggtgagg cgacagggtc tcgatctcgg
     1561 catagggata tcgaccggcg ggtttgcgac ggcggcgacg atggcgcgga ttgccgacct
     1621 ctgccgggtg atcaccctcg agatcaaggc atggtcggac ccggtccacc gcgccctcac
     1681 gggagcgccg gtggccccgg tgctcaggaa cgcagaatac ctcgcgcgcg agggaagaga
     1741 ccggatccgg gtcttccgaa cggtggtgat cccgggtatg accgatagcg aggtgggcgc
     1801 catcgcaaca ttcatcgcct cgctcgaccc gggcataccc taccggctca tcgggtttcg
     1861 gccaaacaac gttctctact accaccccgg accgtcagca gaagagatgg atcggctctg
     1921 tgcgatctct cgcgaagcgg ggctcgatga tgtggcctgg agcggctact accccgaaac
     1981 ggtgcccgac ggcatcaggg ccctcgcggc acgccttgcc gatgcatacg ggggagactc
     2041 tgcagcggcg ctcaccgccg catatgccgc ggcgtgcggg tgccctaccc acccccgcga
     2101 ctgcggggcg tgcccgctcc gcggcaattg cccggcaacc ctccgggagc cctggcgcgg
     2161 ccgataaaac ggctgcctct cccggggccc ctgtgaacgg atcggaaaaa agattgggtg
     2221 ctgatttatt cagtcgccgc tgcgggctgg ctgaagtatt cggccagggg ttttgcgccg
     2281 ctctgtgcta cggcggcgtt gatctggacg aaatcggcgc tgatctccct tccacgcacc
     2341 gactttctgc gacgctctcc ctccatgacc gggtggaacc cgacgcccct ggagaggagg
     2401 agcctccggc gtgccggccc gggaaggtcg cggcgtgcgg gaataccggt cttatccgtg
     2461 ccgccggtga gcctgatcgt atagcccggg agaccgagga cgctcccgtc gatctcgtcg
     2521 ccgatgcgct tgccgatgag cgctcctgcg gcagggccgg tcgcatcaat cttgtatgaa
     2581 cgcccggtac ccgggtctga gaggatgatc ttgaaatctg ccatgttgac gactccttcg
     2641 aaagtatcta tacaatttga taatatggta ttaaaagcct acctccgcgg ggttg
//
LOCUS       DAOSII010000224         2689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_32913_length_2689_cov_41.394837, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000224 DAOSII010000000
VERSION     DAOSII010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32913_length_2689_cov_41.394837"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..1838)
                     /locus_tag="PLV90_10580"
     CDS             complement(156..1838)
                     /locus_tag="PLV90_10580"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HOF97601.1"
                     /translation="MPASRRSHSLTRIFQQAAGKKHIVASQTPLIAIVAASFLDRIEF
                     VETIDAALDRDPDRWRVSPGNLAKGIVLLPFIHSGPRLPILNVSECYQGMDMELLFTS
                     EVRPEWLTRDALACMLDGLSAAGCEHLFTSLALRVYTAFSIPLYPVLHGEATPISLYG
                     VYGEVEGSPLSAPAASREERRRDPVTVTLGQACDPRGIPLITTVHGGDEADSAWSADV
                     IRTLAELNPFPRTDVTAIADPKLATAQNIRTLQKKGFRFVTRCPASFGKKVAARVTRA
                     AYEADAWIPVGSCRDTGEDTLEACDVQEFAKATDDGVSCRFLVFRDNCQRHKFEADLA
                     KKREEFVAVLKEVAGREFAREPDARQALEEARKRLRRYSPWEADLAIETAVTGKDSRA
                     AGWAIRAGDPQLQQEVYLAELHGAESFVLMTNVPGEDLPAREVLCLYTGQKVVRDNFS
                     VIRRPAMVDTLYLEKPGRIAALGTLLSATLLIQMVIRVLVRRNLDALPEPPGLDHGCK
                     PLIRPGLKKILRFIGYYSIITTGGERRFWCISSSHEKNLEIWLRLLELDKGR"
     gene            complement(2498..>2689)
                     /locus_tag="PLV90_10585"
     CDS             complement(2498..>2689)
                     /locus_tag="PLV90_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97602.1"
                     /translation="ACTSLFGGPELTDIPSNDRIPDIHLLDTGRSYGIGYHPIPLMTE
                     GTEMAIEIPGAGDCREGRG"
BASE COUNT          527 a          803 c          882 g          477 t
ORIGIN      
        1 aggttgcaga tgctcgatag cgaggcaact tgaaattcgc aaccgtgcca tcgcccgtgc
       61 ggttcgagaa ccgggtctga tcgagcaatg ggggtgcagg ctcgtgacgg ccggcgctga
      121 cggcgccgca acggtcgagg agaagagcgc agatgtcacc tacctttgtc gagctccagc
      181 aggcgcaacc agatctccag gttcttctca tggctcgacg agatgcacca gaaccggcgt
      241 tcgccgccgg tggtgatgat cgagtagtag ccgatgaacc ggaggatctt cttcaagccc
      301 ggccgaatga gcggcttgca gccatgatcg agccccggcg gctctggcag agcatccagg
      361 tttcggcgca ccagcacccg gatcaccatc tggatcagga gagtcgccga cagcagtgtc
      421 ccgagcgccg caatccgccc gggtttctcc aggtaaaggg tatcgaccat cgccggccgc
      481 ctgatgaccg agaagttgtc cctgaccacc ttctgccccg tatacaggca gagaacctca
      541 cgtgccggca gatcctcccc cggaacgttt gtcatcagga caaacgactc ggccccgtgc
      601 agctcggcca ggtagacctc ctgctgcaac tgcgggtcgc cggcccggat cgcccacccg
      661 gcggcccgtg aatccttccc ggtgaccgcc gtctcgatgg ccagatccgc ttcccagggc
      721 gaatacctgc ggagcctctt acgtgcctct tcgagggcct ggcgtgcgtc aggctcgcgg
      781 gcaaactctc tcccggccac ctccttgaga actgcaacga actcttcccg cttctttgcc
      841 agatcggcct cgaatttatg tctctggcag ttgtcccgga aaacgaggaa acggcaggat
      901 accccgtcat ctgttgcttt tgcgaactcc tgtacgtcgc aggcctcaag cgtatcctca
      961 ccggtatccc ggcatgaccc gacaggtatc caggcatccg cctcgtaggc cgcccgggtc
     1021 acccgggcag ccaccttctt gccgaaactt gcgggacacc gggtgacgaa ccggaagccc
     1081 ttcttctgga gcgttcggat gttttgggcg gttgccagtt tcggatcggc gatggcggtg
     1141 acatcggtac gggggaacgg gttcagctcg gcaagggtcc ggataacgtc tgcgctccag
     1201 gcagaatctg cctcgtcccc gccatgaacg gtggtgatga gcgggatacc gcgggggtcg
     1261 caggcctggc cgagcgtgac cgtgacggga tcccttcgcc gctcttcgcg gctggcggcg
     1321 ggagccgaaa ggggagagcc ttcaacctcc ccgtagacgc cgtacaggga gatcggggtg
     1381 gcttcgccat ggaggaccgg gtacaacgga atactgaacg cggtgtagac ccggagggca
     1441 agggaggtga agaggtgctc acacccggcg gcagagagcc cgtccagcat gcacgcgagt
     1501 gcatcccgtg tcagccactc ggggcgcacc tcggaggtga agaggagctc catatccatc
     1561 ccctggtagc attcggagac attgaggatg gggaggcgcg ggccggaatg gatgaacgga
     1621 agcaggacga tacctttcgc cagattaccg gggctcaccc gccaccggtc cggatcccgg
     1681 tcgagtgccg catcgatggt ctcgacgaac tcgatccggt cgaggaacga agcggcgacg
     1741 atggcgatga gaggcgtctg ggaggcaaca atgtgcttct tcccggcagc ctgctggaat
     1801 atgcgggtca gagaatgact acgtctggaa gcgggcacag gaagtgtaaa gtggggtaat
     1861 gcttatgttt ttcgatccgt gaggaatcgc atgatgcttc agcagggggg ttgcgaagcg
     1921 agcgatcccg gatgagggcg ataggatcga ggaggctccg gggcgacctc cggtcgctct
     1981 ccggcccgaa tgccggccgg ttatcgcaag gatcccgggc cgcttctccc ggatcggatg
     2041 cggcatgacg gttcaatacg ccgcatcgga agagcgggaa gcccccctcg cgggtcgtgg
     2101 caggccggct ccgatgcccc cgcggctaga gagagcccgg caaacgatcc ccgtctggca
     2161 ggtttggcga ccaaacggag gatgcagcgg cgctcatcac cggcgatggg caccgggctg
     2221 ccacgatcgc agagatgttc gcgggattag tgacggcgaa gagtcgctga gagatgggcg
     2281 gtgatgctct tttgagtccg cgctatgccg caggatcggt acgagggcga ctctataccg
     2341 tgcagccctg agaatgcttg atagcccgtg aataccatac gtttacccgg gaatcatcat
     2401 gatggcgaag aaggatgcgg ggaacaggca gacttccctc aactcttgct ccgggggaga
     2461 ggcggtatcc agagcgaaag gaacgccggc ggacagatca gcctctgccc tccctgcaat
     2521 cgccagcgcc agggatctcg atcgccatct ccgtaccttc cgtcatcaac gggatggggt
     2581 gataaccgat accgtacgac ctgcccgtgt cgaggaggtg aatgtcgggg atacggtcgt
     2641 tcgaaggtat atcggtgagt tctggaccgc caaacagaga ggtgcaagc
//
LOCUS       DAOSII010000225         2688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_32934_length_2688_cov_53.380934, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000225 DAOSII010000000
VERSION     DAOSII010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32934_length_2688_cov_53.380934"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PLV90_10590"
     CDS             complement(<1..236)
                     /locus_tag="PLV90_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HOF97603.1"
                     /translation="MILTTTAEVPGYAIGEILGVVFGNTVRTKNIGKDITAGLKSLVG
                     GELEEYTSMLSDARTESYNRMVNAARDLGADAVV"
     gene            complement(240..395)
                     /locus_tag="PLV90_10595"
     CDS             complement(240..395)
                     /locus_tag="PLV90_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97604.1"
                     /translation="MRNQVRWGIALTVIGVVFALLVPIFGIPLVVIGIALLVWRGRED
                     IIEEIKG"
     gene            562..1413
                     /locus_tag="PLV90_10600"
     CDS             562..1413
                     /locus_tag="PLV90_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOF97605.1"
                     /translation="MYKVELATRLADRVYEYWISAPQVARHARAGQFVILRLHEKGER
                     IPLTISAVRGDAVRVIFMAVGKTTQELATLRAGDSISDVVGPLGKPSEIENYGTCCVI
                     GGGVGIASTPLIAEELKAAGNHVIGIIGARNADLLILEDEMRKICDELYITTDDGSKG
                     VHGFAADVLKKLLAERKIDRVWIIGPAIMMKVTSGATVPYGVKTYVSLNPIMVDGTGM
                     CGSCRVTVGGETKFACVDGPEFDAHQVDFNELMQRQRIYTGQEKVALERFAEHQCRCG
                     EGGHHHG"
     gene            1406..>2688
                     /gene="gltA"
                     /locus_tag="PLV90_10605"
     CDS             1406..>2688
                     /gene="gltA"
                     /locus_tag="PLV90_10605"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOF97606.1"
                     /translation="MGDRPADIRIRDFKEVDSGLTADEAIAEAERCLQCKKPLCIKGC
                     PVCIDIPAFVRKIAEGDFPGAARAIKEQNMLPAICGRVCPQETQCEGVCILGNKETAI
                     RIGALERFVADRERENGAELPDVARPTGKRVAIVGSGPAGLTAAAELARAGHAVTIYE
                     SLHEAGGVLTYGIPAFRLPKDVVKAEIDQVLALGVRLKKNHLVGRSISTDELLGHDAV
                     FLATGAGLPAFMGIPGENLNNVYSANEFLTRVNLMHAEAFPEFDTPVMHAARVAVIGG
                     GNVAMDAARVARRLGAKVSLIYRRGEEEMPARRAEVVHAKEEGIEFVTCANPTRILGE
                     GCVTGIECVRMSLCDIDASGRRSPEPIRGSEFTLDVDMVIQAIGTSPNPLLVSLIPGL
                     ERARRGNVVVDEDGRTSIPHVYAGGDIATGAATVI"
BASE COUNT          533 a          833 c          879 g          443 t
ORIGIN      
        1 ttcaccaccg catcggcccc gaggtcgcgc gcggcgttga ccatccggtt ataggactcg
       61 gtcctggcgt cggagagcat cgacgtgtac tcctcaagtt cgccgccgac caggcttttc
      121 aatccggcag tgatatcctt ccctatattc ttcgtccgta ccgtgttacc aaagaccacg
      181 ccgaggatct ctcctatggc gtagcccggc acctctgcag tcgtcgtcag tatcatgatt
      241 caccctttga tctcctcgat gatgtcctcg cgccctcgcc atacgagcag cgcgatgccg
      301 atcacgacaa gcgggatgcc aaagatgggc accagaagcg cgaacaccac gccgatgaca
      361 gtcagtgcga tcccccagcg tacctggttc cgcatactta tttctgcgac tgattcgggg
      421 ataattcttc gtattttccc ttttcccgga gacctacctc gcgcagaaca gatgcagtaa
      481 agaggctgga gtcacaatgt gtacgaagcg tgagatacag ttcgatgctg gtatcgacca
      541 ggataatcag gagtgaagcg gttgtataag gtggaattgg cgaccaggct cgccgaccgg
      601 gtttacgaat actggatatc cgcgccgcag gtggcgcggc atgcacgggc aggccagttt
      661 gtcatcctgc gcctgcatga gaagggtgag cggataccgc tcaccatctc cgcagtcagg
      721 ggagatgccg tccgggtgat atttatggcc gtcggcaaga cgacccagga acttgcgacg
      781 ctccgggcgg gggacagcat cagcgacgtc gtgggaccgc tcggcaaacc gagcgagatc
      841 gagaactacg gcacctgctg cgtcatcggg ggcggagtcg ggatagcgag cacgcccctc
      901 atcgcagagg agctcaaagc ggcaggtaac cacgtcatcg ggatcatcgg ggcgcgaaac
      961 gccgacctcc tcatcctcga ggacgagatg cggaagatct gcgacgagct ttacatcacg
     1021 accgacgacg ggagcaaggg cgttcacggg tttgcagccg acgtcctgaa gaaactgctc
     1081 gcagagagga agatcgaccg ggtctggatc atcggccccg ccatcatgat gaaggtcacc
     1141 tccggcgcga cggtccccta cggcgtcaag acctacgtga gcctcaaccc gatcatggtc
     1201 gacgggacgg ggatgtgcgg ctcctgccgg gtgaccgtcg gcggcgagac gaagttcgcc
     1261 tgcgtcgacg gccccgagtt cgatgcccac caggtcgact tcaacgagct gatgcagcgg
     1321 cagcggatct acaccggcca ggagaaggtc gcgctcgagc ggttcgcgga gcaccagtgc
     1381 cggtgcggcg aagggggtca ccaccatggg tgaccggccg gccgacatcc ggatcaggga
     1441 tttcaaagag gtcgattccg gcctcaccgc ggacgaggcg atcgccgagg ccgagcggtg
     1501 cttgcagtgc aaaaaaccgc tctgcataaa gggttgcccg gtctgcatcg atatccccgc
     1561 gttcgtccga aagatcgcgg agggcgactt ccccggggct gcacgggcga tcaaggagca
     1621 aaacatgctc cccgccatct gcgggcgggt ctgcccccag gagacgcagt gcgagggagt
     1681 ctgcatcctc ggcaacaagg agacggcgat acggataggt gcgctcgagc ggttcgtggc
     1741 cgaccgggag cgagagaacg gcgctgaact ccccgatgtg gccagaccga ccggaaaacg
     1801 ggtggcgatc gtgggatccg gaccggcggg gctgacggcc gcggcggagc ttgcccgcgc
     1861 gggccacgcc gtcacgatct acgaatcact ccacgaagcc ggcggcgtcc tcacctacgg
     1921 catccctgcg ttccggttgc ccaaagatgt cgtcaaagca gagatcgacc aggtgctcgc
     1981 cctcggcgtc cggttgaaga agaaccacct cgtgggaagg agcatcagca cggacgaact
     2041 cctcggccac gatgcggtct tcctcgcgac cggggcaggg ctccctgcgt tcatgggcat
     2101 tccgggggaa aacttaaaca acgtctactc ggccaacgag ttcctgacac gggtaaacct
     2161 gatgcacgcc gaggcgttcc cggagttcga cacgccggtc atgcatgccg cccgggtcgc
     2221 ggtcatcggc ggcggcaacg tcgcgatgga tgcggcgagg gtggcgcgcc gcctgggggc
     2281 gaaggtctcc ttgatctacc ggcggggcga ggaggagatg ccggcacgga gggccgaggt
     2341 cgttcacgca aaggaagaag ggatcgagtt cgtcacctgc gcaaacccga cccggatcct
     2401 cggtgaaggg tgcgtcaccg gtatcgagtg cgtgaggatg tccctctgcg acatcgacgc
     2461 gagcggccgg cggtcccccg agccgatccg gggaagcgag ttcaccctcg acgtggatat
     2521 ggtcatccag gcgatcggga cgagcccgaa cccgctcctc gtctccctca tcccgggcct
     2581 tgagcgcgcc cggaggggga acgtcgtcgt cgacgaagac ggccggacgt ccatccccca
     2641 cgtctacgcc ggcggggaca tcgccaccgg cgccgcgacg gtgatcga
//
LOCUS       DAOSII010000226         2637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_33788_length_2637_cov_39.811774, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000226 DAOSII010000000
VERSION     DAOSII010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33788_length_2637_cov_39.811774"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="PLV90_10610"
     CDS             complement(<1..700)
                     /locus_tag="PLV90_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97607.1"
                     /translation="MDIEAFFAHEGVDVSSRVGIEDLTDADRASVREFLPAARSVIVF
                     GKEVPAPVYRMPEREKTREMLRIAASLDETASRLAGCLDAEHIPARVIPLYFPVRVVD
                     EKVQGIVRLKQIAAAAGLGDIGKNTLLLHRRFGPRLLLAGVVTGEPASGKTAAAAPVC
                     TGCGACIRACPAGALGPAGVDAFRCRNVRAWVPPPVVPAAKWLLRRQFLLRSAAPLAP
                     LVARAATIRCSRCVT"
     gene            complement(1009..>2637)
                     /locus_tag="PLV90_10615"
     CDS             complement(1009..>2637)
                     /locus_tag="PLV90_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HOF97608.1"
                     /translation="DETEDLDPGTKENVARALWMSGYAPESIPPDVMSSLCGTKNHRF
                     GDLMRRRLADLLANPERFTPDYTERYTTFCNHARDLSAHQAYAMTNLLGLDSARGYQE
                     LPQEITFTFPCDDRPQFEYQVGWHFFVGTASDAGGREFGIQFMLWSYSLLPPEMARDE
                     GLSDIENQIVEIHLAVTPANDRHHRPRPVLVAGTTGLIRFSENPYEYAVGKNTMTSLA
                     DDSLFPVRLRARGIDEREDAPVEIAIDLTLHQTKGYILNGDGGLAPSCGGVGTLYYSV
                     TNLRIRPGESWLSIDGTRVPLTGGKFWYDHQWGTGFMPPGSPRSDLLRAVGHLHEQGP
                     GGWDWMAIQFDDDTEIALSALHTNDNRAFYSQTGAEPPTMAAGAKGSYIRQNGEYESI
                     TAEIRVTDWIRSAVADGPYLATNTWYPNRVEVTVYEDAVPAKKRHFVMVPIVTTGQQG
                     FFAAGSEYSEGAVTIESADGERIGIGFLESTGYIDARRQGLLLAGLPDTDDMIRLVSP
                     PAVPDEMKAEVLALFQDPEIVAKLEEELAKCKGL"
BASE COUNT          463 a          839 c          845 g          490 t
ORIGIN      
        1 cggtgacgca gcggctgcac cggatcgttg ccgcccgggc gaccaggggc gcaagcgggg
       61 ccgcacttct gaggaggaac tgccgccgga gcagccattt tgcggccggg acaaccgggg
      121 gtggaaccca tgcacgcacg ttccggcacc ggaacgcatc gacaccggcc ggccccagcg
      181 ccccggccgg gcatgcccgg atgcaggccc cgcacccggt gcagaccggt gcagcggcgg
      241 cagtcttgcc ggaggccggc tctcccgtca cgacaccggc aagcagcagc cgcgggccga
      301 accggcgatg gaggaggagg gtgttcttcc cgatatcccc gagccccgcg gccgccgcta
      361 tctgtttcaa gcggacgata ccctgcactt tttcgtcgac gacccgcacg gggaagtaga
      421 gcgggatgac gcgggccggt atatgctctg cgtcgaggca gccggcaagc cggctggcgg
      481 tctcgtccag gcttgccgca atccggagca tctcacgggt cttctctctt tccggcatcc
      541 ggtagaccgg ggccgggact tccttcccga agacgatcac ggaccgggcg gccgggagga
      601 actcccgcac cgatgccctg tccgcgtcgg tgagatcctc gatgccgacc cgggagctga
      661 cgtcgactcc ttcgtgcgca aagaacgcct cgatatccat tccggcactt cctggagtgc
      721 atgggcgcga acgccttatc aaggtattgg tgaggcgttc ccgccccgga tagtcatcaa
      781 gaccctggag aagaaggggt ctgggctgga ccgggtgaag attaccgggc agttaccaaa
      841 aaatacttcc cggtccggtc accgcagcct tcggggcgac cacccgggaa acccccggtg
      901 tagcgcaccg aggaacgact tacgcgtgct cgatccctct tcctgattca ctgatgcagc
      961 tacctgcggg atgatggctc cggatgaggt ttgaccggcg ggaagggctc acaacccctt
     1021 gcacttcgcc agctcctcct caagcttcgc gacgatctcc gggtcctgga acaacgccag
     1081 tacctcagcc ttcatctcat ccggaaccgc gggcggggag acgagccgga tcatatcgtc
     1141 ggtatcgggc aggcccgcga ggagcaggcc ctgcctccgg gcatcgatgt agccggtaga
     1201 ctctagaaat ccgatcccta tcctctctcc gtcagcagac tcgatggtga cggcgccctc
     1261 ggagtactcg gaacccgcgg caaagaaccc ctgctgcccg gtcgttacga taggcaccat
     1321 cacaaagtgc cgtttcttgg ctggaacggc atcctcgtat accgtcacct ccacgcggtt
     1381 cgggtaccag gtgttggtgg ccaggtacgg gccgtccgcc accgcgctcc ggatccagtc
     1441 ggtcacccgg atctcggcgg tgatcgattc atactctccg ttctgccgga tgtacgatcc
     1501 ttttgcacct gcagccatcg tcggcggttc tgctccggtc tgtgaataga acgcccggtt
     1561 atcgttggtg tggagcgcgg agagggcgat ctcagtatcg tcgtcaaact ggatcgccat
     1621 ccagtcccac cccccggggc cctgctcgtg gaggtgcccg actgcacgca gtagatcgct
     1681 ccgggggctt cccgggggca tgaagcccgt gccccactgg tgatcgtacc agaactttcc
     1741 gccggtgagc gggacccggg tgccgtcgat ggagagccag ctctcgccgg gtcgtatccg
     1801 gaggttcgtg accgagtagt agagcgtgcc gacaccgccg caactgggtg ccaggcctcc
     1861 atccccgttc agaatgtagc ctttggtctg gtgaagggtg agatcaatgg cgatctccac
     1921 gggggcgtct tcccgttcat cgataccccg ggctctcagg cggacgggga agagggagtc
     1981 atcggcaaga gaggtcatcg tgttcttgcc gacggcatac tcgtacgggt tctccgagaa
     2041 ccggatgagg ccggtggtgc ccgcgacaag aacgggcctc ggtcgatgat gccggtcgtt
     2101 tgccggagtt acggccaggt ggatctcgac gatctggttc tctatgtcgc tcaggccctc
     2161 gtcccgggcc atctccgggg ggaggagaga gtaactccag agcatgaact ggatgccgaa
     2221 ctcccggcca cctgcgtcgg acgccgttcc cacgaaaaag tgccatccga cctgatactc
     2281 gaactgcggc ctgtcgtcgc aggggaacgt gaacgtgatc tcctggggca gctcctggta
     2341 tccccgtgcg ctatccaggc caaggaggtt tgtcatggca taggcctggt gtgccgagag
     2401 atcacgagca tggttgcaga aagtagtgta ccgttcggtg taatcggggg tgaaccgttc
     2461 gggattggcc agcagatcgg caagacgccg ccgcatcaga tccccgaatc gatggttttt
     2521 cgtgccgcag aggctgctca tgacatccgg cgggatcgat tctggtgcgt atccgctcat
     2581 ccagagcgca cgagcaacgt tctctttcgt tccggggtcg agatcctccg tttcatc
//
LOCUS       DAOSII010000227         2628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_33937_length_2628_cov_46.654100, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000227 DAOSII010000000
VERSION     DAOSII010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33937_length_2628_cov_46.654100"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..>2628)
                     /locus_tag="PLV90_10620"
     CDS             complement(5..>2628)
                     /locus_tag="PLV90_10620"
                     /inference="COORDINATES: protein motif:HMM:NF023252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOF97609.1"
                     /translation="AVPNYGYTGKGFDVAVSGTLHGNLTYYEFSDYTGLIHAGENREF
                     TIDVPTPANGTIRYARLYIYTTWGHDEKRKEGTPVRIGLAVDGKNFIADRVYNDRKDE
                     GIYNYLLETHAFNVTGVPGGIAGEHVVKLTNDGRKDDVFAAYGVGLLVVTEDPAEPEM
                     TYWIAEGSDTLYANPDFGTTSEDCITTVVFNESIDLSGTAEARVILVATAGSGMDDDE
                     HRIYFNDGEWFNLLTAGSSAISVADLDVRPFLRQSGNEGGIQSLITATKGDYMENRAL
                     ILAVWKGTPPEGYEASATSTTGTPGTPAASTGESGSRFTLNATNGRIEVTHLVADGGA
                     VQVYIPEGAVITEGAGLISALTLQRLLPGDAGGADPAYAVGNEDMKSDLPLRLVISVS
                     KSGGVIARYDRSSDTWRPLETKSDAAGSVSTTIRQGGIYAVRYGEAAPGGLLGLLFGA
                     VQALFGLFGFTFAGEEPGPALAAEPTSEPRSASAEPASTVTPVPAVDIATMRFALSVL
                     SDPPGALIDLDGEYTGRTTPAAFAGLSGGTHTIRVCVDGAEPVEREIQLARDDEVVVE
                     IPGSASPGLLRTWEQNHYGGVYVDSFPEGAEIYVDGRKVNRKTPDTIYGLKEGLHTIK
                     VKKEGTVFSSDKERVWINKNALVRVMFTSGNAELTRSITFESDEHAQQPFSINGRYLG
                     DRLPKTVEIRGIGGAYLTLSDGESYRTHRIPTTAQSNDTVKAAFSDAPCSLLVESTPA
                     GAAISIDGFLTKFSTPYVVSNISEGRHLIGVSKPGHIPVEREVLLMAGSGDATVKLTL
                     KPYTWGSLEVSSNPAGAKIYLYGRDTGEVTPHTFHYLDIGSYSVKVVGKEGSKTIEDV
                     LVSPYTTTECHAEITGG"
BASE COUNT          432 a          865 c          812 g          519 t
ORIGIN      
        1 cctctcagcc ccccgtaatc tcggcatggc attcggtggt cgtatacggc gagaccagca
       61 cgtcctcgat cgtcttcgac ccctccttcc ccaccacctt caccgagtaa ctgccgatat
      121 cgaggtagtg gaaggtatgg ggcgtcactt cgccggtatc gcggccatag aggtatatct
      181 tggcgcccgc cgggttgctt gagacctcaa gcgaccccca ggtatagggt ttgagggtga
      241 gcttcaccgt ggcatcgccg gaaccggcca ttagcagaac ctcccgctca accggtatgt
      301 gtcccggctt tgagacccct atcaggtgtc tcccttccga tatgttgctg acgacatacg
      361 gcgtggagaa cttcgtcagg aacccgtcga tcgatatcgc cgctcccgcc ggcgtcgact
      421 ccacgagaag gctgcagggc gcatcggaga acgcagcctt cacggtatcg ttcgattggg
      481 cggtggtggg tatcctgtgc gtccggtaac tctcgccgtc agagagggtg agatatgccc
      541 ccccgatccc cctgatctcg accgtcttcg ggagccggtc gcccaggtac ctgccgttga
      601 tcgagaacgg ttgctgcgcg tgctcgtccg actcgaacgt gatcgagcgc gtcaactccg
      661 cgttgccgct ggtaaacatc acccgcacca gggcgttttt attaatccag acccgttctt
      721 tgtcggaact gaacacggtc ccctctttct tcaccttgat ggtgtgcagc ccctccttca
      781 gcccgtagat ggtgtcgggg gtcttccggt tcaccttgcg gccgtcgacg tagatctctg
      841 ccccctccgg gaacgagtcg acgtagacgc cgccgtagtg gttctgctcc caggtgcgga
      901 gcaggccggg ggaagcggaa cccgggatct cgaccacgac ctcgtcgtcg cgcgcaagct
      961 ggatctcccg ctccaccggc tcggcgccgt ccacgcacac ccggatcgta tgggtgccgc
     1021 cggagagccc cgcgaaggcg gccggggtcg tcctgccggt atactctccg tcgaggtcga
     1081 tgagcgcgcc cggcggatcg gagagcaccg agagagcaaa ccgcatcgtg gcgatatcga
     1141 cggcaggtac cggtgtaacc gtagatgcgg gctctgccga ggccgatctg ggttcagacg
     1201 tgggctccgc cgccagcgcc ggtcccggct cctccccggc gaaggtgaag ccgaagagcc
     1261 cgaagagcgc ctggacggca ccgaacagca ggccgagcag cccgcccggg gccgcctcgc
     1321 cgtaccgaac cgcgtagatg ccgccctgcc ggatcgttgt cgagacgctg cccgccgcat
     1381 cgctcttcgt ctccagcggc cgccaggtgt cggaagagcg atcgtacctg gcgatcacgc
     1441 ctcccgactt gctcacggag atgaccagcc tgagcgggag gtcgctcttc atatcctcgt
     1501 taccgaccgc gtaggccggg tcggcgcccc ccgcatcgcc gggtaaaagc ctctgcaacg
     1561 tcagcgccga gatgaggccg gcaccttcgg tgatcaccgc accctcgggg atgtacacct
     1621 ggacggcccc gccgtcggcg acgaggtgcg tgacctcgat ccgcccgttc gtcgcgttga
     1681 gggtgaaccg cgagccggac tcaccggtcg atgccgccgg agtcccgggc gtgcctgtcg
     1741 tggacgtggc gcttgcttcg tagccctccg gtggggtgcc cttccagacg gcgaggatga
     1801 gggcgcggtt ctccatgtaa tctcccttcg tcgcggtgat aaggctctgg atccctccct
     1861 cgttccccga ctgccggagg aacggtctca cgtccagatc ggccacgctg attgccgaag
     1921 agcctgcggt gagcaggttg aaccactcgc cgtcgttgaa gtagatccgg tgttcgtcgt
     1981 cgtccatccc cgaaccggcc gtggcgacca ggatgacgcg cgcctcagca gtcccggaga
     2041 ggtcaataga ctcgttgaaa accaccgtcg tgatacagtc ttcagacgtt gtcccgaagt
     2101 ccgggttggc atagagcgta tccgacccct ccgcgatcca gtaggtcatc tccggttcgg
     2161 cagggtcttc ggtgacgacc aggaggccga cgccgtaggc tgcaaagaca tcgtccttcc
     2221 tcccgtcgtt cgtcagtttc acgacgtgct cgccggcaat gccgcccgga acgccggtga
     2281 cgttgaacgc gtgcgtctca agcagatagt tgtagatccc ctcgtctttt cggtcgttat
     2341 agaccctgtc ggctatgaaa ttctttccgt cgaccgcgag gccgatcctg accggggtgc
     2401 cttctttcct cttctcgtcg tgcccccagg tagtataaat gtagagccgc gcgtaccgaa
     2461 tcgtgccgtt cgcaggggtg gggacatcga tcgtaaactc cctgttctcc ccggcatgga
     2521 tgagcccggt gtaatcgctg aactcgtagt aggtcaggtt cccgtggagg gtgcctgaga
     2581 cggcgacatc gaaccccttt ccggtgtagc cgtagttggg aactgcgc
//
LOCUS       DAOSII010000228         2628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_33938_length_2628_cov_46.562379, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000228 DAOSII010000000
VERSION     DAOSII010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33938_length_2628_cov_46.562379"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1177
                     /locus_tag="PLV90_10625"
     CDS             149..1177
                     /locus_tag="PLV90_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HOF97610.1"
                     /translation="MSSNAVLPDGYPAFLSGLKVRIREARVRAALSVNRELILLYWEI
                     GRDILQRQQEEGWGAKVIERLSTDLRKEFPDVKGFSPRNLKYMRSFAEAWPDPVFVQQ
                     VAAQIPWFHNCVILDKLSNREFREWYIHKTIENGWSRNVLVHQIESGLIEREGRAVTN
                     FSSALPAPQSDLARAMLKDPYIFDFLGIGEDAAERELERALLDHIREFLLELGVGFAF
                     VGSQYRLDVGGKDFSIDLLFYHLKLRSYVVIDLKIGEFLPEYAGKMNFYLSAVDDLLR
                     HLDDNPSIGIVLCKSRNRIIAEYALRDMEKPIGVAGYELTTSLPEDLRGRLPTVDELE
                     EELRRGEE"
     gene            complement(1380..1520)
                     /locus_tag="PLV90_10630"
     CDS             complement(1380..1520)
                     /locus_tag="PLV90_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97611.1"
                     /translation="MRRRCREREWQAARLPEGLREEIMERIVVEERGSGVLVKLRKGE
                     GF"
     gene            1573..1845
                     /locus_tag="PLV90_10635"
     CDS             1573..1845
                     /locus_tag="PLV90_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOF97612.1"
                     /translation="MSKTIITVVGKDAVGIIAKICTYLAGNDVNVEDISQTIVQGYFN
                     MMMIVDTGASTKPYAAMVAELDELGNAIGVRIRCQREDIFTKMHRI"
     gene            1854..>2628
                     /locus_tag="PLV90_10640"
     CDS             1854..>2628
                     /locus_tag="PLV90_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HOF97613.1"
                     /translation="MITILEVNETNKMIEQEKLDVRTITLGISLLDCCDADLDALNRN
                     IYDKIAGLARDLVSTGREIELEYGIPIVNKRIAVTPVALVGGRACRSPEDFVEVAKTL
                     DRAARDTGVNFIGGYSAIVSKGMTPTDETLIRSIPAALAATERVCSSVNVGSTKTGIN
                     MDAVKLMGEIVRETAEATKERNSIGCAKLVVLCNAPDDNPFMAGAFHGVSEADAVINV
                     GVSGPGVIKHALEGVRGANFEILCETVKRTAFKVTRAGQL"
BASE COUNT          571 a          751 c          807 g          499 t
ORIGIN      
        1 cctgtggagc caagacctct acatcacatc tcggtggtgc aagtgttggc catgatagcc
       61 gagaagcccc gcccaaaagg cgcggggtag ttcacagagt cggaacggct ggaagggaag
      121 attcggggga atttgaaggg gcttgggtat gagttctaat gcggtacttc ccgatggcta
      181 tcccgccttc ctctccggcc tgaaggtgcg gatccgcgag gcccgggttc gggcggccct
      241 ctcggtgaac cgggagttaa ttctgctcta ctgggagatc gggcgcgaca tactgcagag
      301 acagcaggag gaggggtggg gagcgaaggt gatcgaacgg ctctcgactg atctcaggaa
      361 ggaattcccg gatgtcaagg gtttctcccc ccggaacctg aagtatatgc ggtcgtttgc
      421 ggaagcatgg cccgaccctg tattcgtgca gcaggttgct gcacaaattc catggtttca
      481 taactgtgta atcctggaca aactgtcgaa ccgggagttc cgggagtggt atatccacaa
      541 gaccatcgaa aacggatggt cgcggaacgt cctcgtccat cagatcgaga gcgggcttat
      601 cgagcgcgaa gggcgggcgg tgacgaactt ctcttcggcg ctccccgccc cgcagtcgga
      661 cctcgcccgg gcgatgctca aagatcccta tatctttgat ttcctcggga tcggggagga
      721 tgccgccgag cgggaactgg agcgggcgtt gctcgaccac atccgtgagt tcctgctgga
      781 gctcggggtc gggtttgcgt tcgtgggcag ccagtatcgg ctcgatgtcg ggggcaagga
      841 cttctccatc gaccttctct tctaccacct gaaactccgg tcctacgtgg tgatcgacct
      901 gaagatcggg gagttcctgc ccgagtacgc gggcaagatg aacttctacc tctctgcggt
      961 cgacgacctc ctccggcacc tggacgacaa cccgagtatc gggatcgtgc tttgcaagtc
     1021 gaggaaccgc atcatcgccg agtatgccct ccgcgacatg gaaaaaccga ttggggtcgc
     1081 gggatacgaa ctgacgacca gcctcccgga ggatctgcgg ggcaggctac cgacggtgga
     1141 tgagctggaa gaggaactga ggcgggggga ggagtgatgg agaagtacgt gctgaatacg
     1201 ctgggaaaaa tagctggttt cctcctcaaa tacccggcaa aattgtcatt ttaacgacat
     1261 aggagactac ctgagtttga cataatcccc ttttgcaagg cttccccggg ttgagaaacg
     1321 ttccaggagc acctgcaatg tcagcactgg cgatacaggt agttgttgcc tgacaactct
     1381 caaaacccct cccccttcct cagtttaacc agaaccccgc tcccgcgctc ttcaacaacg
     1441 atcctctcca tgatctcctc ccggagcccc tccgggagcc ttgccgcctg ccactcccgc
     1501 tcccggcacc gtcgcctcat cggattaatg ccttgagaga cccatcagta tacacagaga
     1561 tcaggagaga gcatgagcaa gacaatcatt accgtcgtcg gaaaggatgc cgtggggatc
     1621 atcgcgaaga tctgtacgta tcttgccggt aacgacgtca acgtcgagga tatatcgcag
     1681 acgatcgtgc agggctactt caacatgatg atgatcgtcg ataccggcgc ctcgacaaaa
     1741 ccgtacgccg cgatggtggc cgagctcgac gagctcggca atgccatcgg cgtccggatc
     1801 cggtgccagc gagaggatat cttcacgaag atgcaccgta tctgaggagt cccatgatca
     1861 ctattctcga ggtgaacgag accaacaaga tgatcgagca ggagaagctc gatgtccgga
     1921 cgatcactct cggcatcagc ctcctcgact gctgtgatgc cgatctcgat gccctgaacc
     1981 ggaacatata cgataagatc gcagggctcg caagagacct cgtctcgacg ggaagagaga
     2041 tcgaactcga gtacggcata ccgatcgtca acaagcgaat tgccgtaacc cccgtcgcgc
     2101 tcgtcggcgg gcgggcctgc aggtcgccgg aggactttgt cgaggtcgca aagacgctcg
     2161 accgggccgc gcgggatacc ggggtcaact tcatcggggg atactcggcg atcgtctcga
     2221 aagggatgac tccgaccgac gaaaccctca tccgctcgat cccggcggct ctcgccgcga
     2281 ccgagcgggt ctgcagctcg gtgaacgtcg gctcgacgaa gaccgggatc aacatggatg
     2341 ccgtcaagct gatgggggag atcgtgcggg agacggccga ggcgacgaag gagagaaact
     2401 ccatcggctg cgcgaaactg gtcgtcctct gcaacgcacc cgacgacaac ccgttcatgg
     2461 ccggagcgtt tcacggtgtt tcagaggccg acgcggtcat caacgtgggc gtaagcggcc
     2521 cgggggtcat caagcacgcg ctcgagggcg ttcggggagc gaacttcgag atcctctgcg
     2581 agacggtcaa gaggacggcc ttcaaggtca cccgcgcagg gcagctcg
//
LOCUS       DAOSII010000229         2621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_34052_length_2621_cov_49.799299, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000229 DAOSII010000000
VERSION     DAOSII010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34052_length_2621_cov_49.799299"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..72)
                     /locus_tag="PLV90_10645"
     CDS             complement(<1..72)
                     /locus_tag="PLV90_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HOF97614.1"
                     /translation="MLRCDLHIHTRFSRDGESSVEEIL"
     gene            116..1540
                     /locus_tag="PLV90_10650"
     CDS             116..1540
                     /locus_tag="PLV90_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase-like protein"
                     /protein_id="HOF97615.1"
                     /translation="MHILLPTGSATAAIVKAAAERFRSRYDIDVAVTGDIASFLAPGD
                     LIELLSGRDYDMAIVSGMCTASFADVERETGVPVYRGPRHAADLALILGVLDRIRLSK
                     TIPADDFLTETRRKEACRRVALREAEAGADFVIRGVKIGGGSRMKVLAEIMDAHRRGD
                     IADEVRRFFAEGADIVDLGFGFDAAPGDVERCFAALEDLDGPLAVDTQDPLLIAAALP
                     RADLVLSLHEGNIPVVGAAIADAGAAAVVVPRERTLRENLAAAGEAGISRLIADPLLQ
                     PVGSGLVDSLSGFPAIPYPLFFGAGNVVELLDADSTGANALLAGMAHEVGAAVIFTSE
                     HSDKTRGSVAEMRRATDMMALMADRPYPKDLGLDLLILKEKRRRREPPLEYGSIVDAC
                     PAPDEIVYDPLGCIRIGIEEDCIVAVHKGRAVRGKHWEDVFYTLLASGSLSRLDHAAY
                     LGKELFKAELAIRLRRSFEQDGPF"
     gene            1759..>2621
                     /locus_tag="PLV90_10655"
     CDS             1759..>2621
                     /locus_tag="PLV90_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOF97616.1"
                     /translation="MRRDPIIFGFITALVIGLVALPALAATAGVDIQGGSYHPASLTV
                     ESNTTVTWTNHDRIGHTVTSTDGLFDSGPIEHNETFNYTFTDTGTYPYGCTLNASTRR
                     TPMQGVVIVVPEGMAVAPDGNRTSGEEPGNATLADLVSGSDLLTTFGEAVGKAGIAQT
                     VLAASGPYTVFAPDDDAFAALGNETVAALFNDTETLDTLLMHHVVRETYTVNDLTAKA
                     NAAGNRTVLTTLTGDNLTVSTTDGNLTVGNATIVASDMAASNGVVQIVDTVLVPPGLV
                     LPENQTAVGNQT"
BASE COUNT          491 a          835 c          828 g          467 t
ORIGIN      
        1 caggatctcc tcgacactgc tctctccgtc tcttgagaac ctggtgtgga tatgcagatc
       61 gcaccgcagc atgagatcag attatttgtc acccgctgaa taataagtga agtgtatgca
      121 catcctcctc ccgaccggat cggcaacggc tgcaatcgtg aaggcggcgg ctgagcggtt
      181 ccgcagccgt tatgacatcg acgtggcggt taccggcgat atcgcatcgt tccttgcacc
      241 cggcgacctc atcgaactgc tttctggccg cgattacgat atggcgatcg tctcggggat
      301 gtgcaccgca tcctttgccg acgtcgagcg ggagaccggt gtcccggtct accggggccc
      361 gcggcatgct gccgatctcg ctctgatcct cggcgttctc gatcggatcc ggctctcaaa
      421 gaccatccct gccgacgact tccttacaga gacgcgccgg aaggaggcct gccgccgggt
      481 ggcgctccgg gaggcggagg caggcgccga cttcgtcatc cggggcgtaa agatcggcgg
      541 aggctcgcgg atgaaggtgc tcgcggagat catggatgcg cacaggcgcg gcgatatcgc
      601 cgatgaagtc cggcggttct ttgccgaagg ggcagatatc gtggacctgg gatttgggtt
      661 cgacgcggcc ccgggggacg tcgaacggtg ttttgcagcc cttgaagacc tcgatggccc
      721 tctcgcggtc gacacccagg accctctcct catcgctgcg gcactccccc gcgccgacct
      781 ggtcctctcc ctgcacgagg gaaacatccc cgtcgtcggg gcggcgatcg cggatgccgg
      841 ggcggcggcg gtcgtggtgc cgcgggagcg aacgctccgg gagaacctgg cggcggcagg
      901 ggaggccggg atctcccgcc tgatcgccga ccccctcctc cagccggtgg gctccgggct
      961 cgtcgattcg ctctccggtt tcccggccat cccctatcca ctcttcttcg gagccggaaa
     1021 cgtcgtcgag ctcctcgatg ccgactcgac gggtgcaaac gcgctgctcg cgggtatggc
     1081 gcacgaagtc ggcgccgccg tcatcttcac gagcgagcac agcgacaaga cccggggatc
     1141 ggtggcagag atgcggcggg caaccgatat gatggcgctg atggccgacc gcccataccc
     1201 caaagacctg gggctcgacc tcttgatcct gaaagagaaa cggcggcggc gagaaccccc
     1261 gctggagtac gggagcatcg tggacgcgtg ccctgcgccc gacgagatcg tctacgatcc
     1321 tctcgggtgc atccgcatcg gcatcgagga ggattgcatc gtcgcggtcc acaagggccg
     1381 cgccgtacgg ggaaagcact gggaagacgt cttttacacg cttcttgcga gcgggagcct
     1441 ctcccggctc gaccacgccg cctacctcgg caaggaactc ttcaaggcgg aacttgccat
     1501 ccggttgcgg cggagtttcg agcaggatgg accgttctga agaaccggat cccgtacttt
     1561 tttcccggtg gcgatggtcg aatccggtcg agagttccgg taattttcaa aaatataata
     1621 atcattatat actatgacaa ttattccaaa ccccgtgcag cggtgagcga cgcgcccgcc
     1681 ggagacccgg gatcgtccgg ctccatcgcc agggagtggc cgctcgccct gtcgggttca
     1741 tacgtgtggt gagactgcat gagaagagat ccaatcatat tcggattcat aacagccctg
     1801 gtcatcggcc tcgtcgccct gcccgccctg gcggcgaccg ccggtgtcga tatacaggga
     1861 gggtcgtacc atcccgcgtc actcactgtc gagagcaaca caacggtgac ctggacgaat
     1921 cacgaccgta tcggccatac cgtcacgagc acggacgggc tctttgactc cgggccgata
     1981 gagcataacg agacgttcaa ctacaccttc accgatacag ggacctatcc ctacggctgt
     2041 acgctcaacg cctcgacgcg gcggacgccc atgcagggcg tcgtcatcgt cgtcccggag
     2101 ggaatggcgg tcgccccgga cgggaacaga acttccggag aggagccggg gaacgcgacg
     2161 cttgccgatc tggttagcgg gagcgacctc ctgaccacat ttggcgaggc tgtcgggaag
     2221 gccgggatcg cgcagacggt gcttgccgcc agcgggccgt atacagtctt tgcgccggac
     2281 gacgacgcct ttgcggctct cggcaacgag accgttgcag cgctcttcaa cgatacggag
     2341 acgctggata ccctgctcat gcaccacgtg gtgagagaga cctataccgt gaacgacctg
     2401 acggcgaagg caaacgccgc cggaaaccgg acggttctca cgacactcac cggggataac
     2461 ctcaccgtca gcacgaccga cggcaacctc acggtgggaa acgccacgat cgtcgcatcc
     2521 gatatggcgg cctcaaacgg tgtcgtgcag atcgtcgata cggtcctcgt gccgccgggc
     2581 ctcgtgctgc cggagaacca gaccgcggtg gggaaccaga c
//
LOCUS       DAOSII010000230         2617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_34123_length_2617_cov_41.248634, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000230 DAOSII010000000
VERSION     DAOSII010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34123_length_2617_cov_41.248634"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..578
                     /locus_tag="PLV90_10660"
     CDS             9..578
                     /locus_tag="PLV90_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 family protein"
                     /protein_id="HOF97617.1"
                     /translation="MGLLTEGINEVIATTEGNAAPMGIINRNGALHMVLFRGSHTAGN
                     IVRDRWVVANFIFDPVLYVRTAFDDLPADAFIREEAGGTAVCRLRDAEAWAAFAADVE
                     RAGDEAIVVRLTPLKEEVLDLRLHPVNRGFASIVDATVHATRYVGNRDPWLGRLIEHH
                     AGLARKCGGPREWEALDLLLRYIADPARE"
     gene            709..1149
                     /locus_tag="PLV90_10665"
     CDS             709..1149
                     /locus_tag="PLV90_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOF97618.1"
                     /translation="MAKIFDMLKNDGRFGRLVDIIQTLGEEKKLEGEGPMTFFAPVDS
                     AWDAIPESNRRMILNDKQMLSHLIDFFTIGGHRCTLEALLARNVVQTVEGNVIAVRKT
                     ERGTQVDEAVVLEGNMEAENGIVHVLDSVPFATLWQAFEAYAAG"
     gene            1201..1635
                     /locus_tag="PLV90_10670"
     CDS             1201..1635
                     /locus_tag="PLV90_10670"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOF97619.1"
                     /translation="MKSILETAREDSRLSTSIRMIEASGLSEALRKEGELTAFIPTNE
                     AFSAFPGEVLDAIAEDRGKLAGMIGYHVVRGKLTAHELARMEAIRTLQGDHLDITGPP
                     MAVRLNDATVIVSDVRCTNGIYHIIDRVLLPRAVEARVKKTI"
     gene            1632..2057
                     /locus_tag="PLV90_10675"
     CDS             1632..2057
                     /locus_tag="PLV90_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOF97620.1"
                     /translation="MKSILATLRDSGSFAVLLDLVQAAAMEERLSTKGPYTLFAPDDG
                     AFGAVPEQELDALREDAARLAGVLNHHIVPGVHSMLDLLGIRVLRSLEGGDLTVEVTR
                     EGVLVNNVPIVESDAWCTNGICHAVSALLLPPGARKVAA"
     gene            2186..2611
                     /locus_tag="PLV90_10680"
     CDS             2186..2611
                     /locus_tag="PLV90_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOF97621.1"
                     /translation="MKNIIETLEDSENLSVFLDLIRMAGMEPMLRERGPFTVFVPTDE
                     AFLRVEKERMDEIRRDADKLLLIVSYHVVPGILTSEDLRSVAAVRSSLGTGIVVRSSD
                     RGITVNSVPIVEADAFCTNGICHAIASALVPPAIEIVTP"
BASE COUNT          471 a          795 c          892 g          459 t
ORIGIN      
        1 gggtggcagt gggactcctg accgagggga tcaacgaggt gatcgcgacc accgagggga
       61 acgccgcccc catgggtatc atcaaccgga acggcgcgct gcacatggtc ctcttccggg
      121 ggagccatac cgccgggaac atcgtgcggg accgctgggt ggtggcgaac ttcatcttcg
      181 atccggtcct ctacgtccgg acggcgtttg acgacctgcc cgccgatgcc ttcatccgcg
      241 aggaggccgg cgggacggcc gtctgccgcc tccgcgacgc cgaggcctgg gccgcgtttg
      301 ccgcggacgt ggagcgggcg ggcgacgagg cgatcgtcgt ccggctcacc cccttgaagg
      361 aggaggtgct cgatctccgc ctgcacccgg tgaaccgggg gtttgcaagc atcgtcgacg
      421 cgacggtcca tgccacccgc tacgtcggga accgtgaccc gtggctcggt cggttgatcg
      481 agcaccatgc cggccttgcc cggaaatgcg gcgggccccg ggagtgggag gcgctcgacc
      541 tccttctgcg gtatatcgcc gatcctgccc gcgagtgacg ggataacgcc gcctcttaca
      601 ggctgagggt gcccgctcgt ctcctccaga tttctatttc gttttttgca taataatcga
      661 tatataccat gacgtgccac gaagggtgct tgcagggagg taccggcaat ggcgaagatc
      721 tttgatatgt tgaagaacga cgggaggttt ggccggctcg tcgatatcat ccagacgctt
      781 ggggaggaga agaagttgga gggcgagggg ccgatgacgt tctttgcccc ggtcgactcg
      841 gcgtgggatg cgatccccga atcgaaccgg aggatgattc taaacgacaa acagatgctc
      901 tcgcacctca tcgacttctt cacgataggc gggcacaggt gcacgcttga agcgctgctt
      961 gcaaggaacg tcgtgcagac cgtcgaagga aacgtcatcg cggtccggaa gaccgagcgg
     1021 ggcacccagg tcgacgaggc cgtcgtgctt gaggggaata tggaggcgga gaacggcatc
     1081 gtccacgtcc tggacagcgt cccctttgcg acgctctggc aggcgttcga ggcgtacgcg
     1141 gcggggtagt cccgggcggg atcccccgcc gtcacccgga acgtagaggg gaggatcatc
     1201 atgaagagca tacttgagac tgcacgggaa gacagccggt tgagcacctc catacggatg
     1261 atagaggcca gcgggctctc ggaggcactc cggaaagagg gagagttgac ggcctttata
     1321 ccgacgaacg aggcgttctc cgcctttccc ggggaggtgc tggacgctat cgcagaagac
     1381 cggggaaagc ttgccggcat gatcggctac cacgtggtcc ggggcaaact cactgcacac
     1441 gagcttgcgc ggatggaggc catcaggacc ctgcaggggg accacctgga cataacgggg
     1501 cccccgatgg cggtgcgcct gaacgatgcg accgtcatcg tatccgacgt caggtgcacg
     1561 aacgggatct accatatcat cgatcgggtg ctcctgccgc gtgcggtcga ggcgcgggtg
     1621 aagaagacga tatgaagagc atccttgcga ccctgcggga ttcgggctcg tttgccgtcc
     1681 tcctcgacct cgtgcaggca gccgccatgg aggagcggtt gagcacaaaa gggccctaca
     1741 cgctctttgc cccggacgat ggcgcctttg gggcggtgcc ggagcaggaa ctcgacgctc
     1801 ttcgggagga tgccgcccgg ctcgccgggg tgctcaacca tcatatcgtc cccggggtgc
     1861 actccatgct cgacctcctc ggtatacggg ttctccggtc gcttgagggg ggcgacctga
     1921 ccgtggaggt gacccgggag ggggtgctgg tcaacaacgt cccgatcgtg gagtcggatg
     1981 cgtggtgcac gaacggcatc tgccacgcag tctccgcgct cctccttcct cccggcgccc
     2041 ggaaggttgc tgcgtagcgg gccgggcggg tgaaccccgg ggagaggata tgaaggccgg
     2101 gtgtctcagg ccgggccggc cgtgcgcagg atctcccccc accccggaag ctacataacc
     2161 cggcaggtgc agtggggaga gcgatatgaa gaacatcatc gagaccctgg aggattcgga
     2221 gaacctctcc gtcttcctcg acctgatccg gatggcgggc atggagccga tgctccgcga
     2281 acgggggcct tttacggtct tcgtcccgac ggacgaggcg tttttgcggg tcgagaagga
     2341 acggatggat gagatccggc gcgacgcgga caaacttctc ctcatcgtga gttaccacgt
     2401 cgtgccgggg atcctcactt cagaggatct ccggtccgtc gccgccgtca ggtcgagcct
     2461 cgggacgggt atcgtcgtgc ggtcgtccga ccggggcatc accgtcaaca gcgtcccgat
     2521 cgtcgaggcc gacgcattct gcacgaacgg catctgccac gccatcgcat cggccctcgt
     2581 ccctccggcg atcgagatcg tcacgccctg accggca
//
LOCUS       DAOSII010000231         2615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_34152_length_2615_cov_51.225781, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000231 DAOSII010000000
VERSION     DAOSII010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34152_length_2615_cov_51.225781"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2000
                     /locus_tag="PLV90_10685"
     CDS             <1..2000
                     /locus_tag="PLV90_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HOF97622.1"
                     /translation="EMDKMAKEDRSALHEAMEQQCYDDKTEVLTESGWKLFCDVTADD
                     RVATLSSEGRLEYTAPSNFVATEYDGEMYYVKSRQVDLAVTPNHRMYVNLNRRADEWE
                     GFRLVRMDEIPIHKRMRFKKGAIWAGERRDVYEIPPVVKFANQNAEGKLTDPVTVGMN
                     DWLEFLGYFLSEGSVQRHSKTGVPYRTIISQKTSESAETIRECLERLPFRFSYDGRNF
                     SINAKQLAEHLAPFGKCHEKYVPGCAKRLPPEQIGILLDALMLGDGYVNKTTGVSIYT
                     TSSKRLADDVTELLLKKGWSGNVHLMREAGTIIANPRGGTSTTTHDIFQVTFIRDGQN
                     EPNINSNGKRHIEKRPYKGTIYCLEVPNHILYVRRNGIPVWCGNSISVAKAGITATLK
                     SRCALLGAANPKLGRFDQFVPIGEQIDMPPSLLSRFDLIFVMTDQPEAQRDGAIAQHI
                     IKTHAVGELIKQHEFSPLPGVDDAYIERALAPVTPDIDPTLLRKYIAYAKRNCFPILS
                     DGAKEALIAYYMRLRNLAGGNKPVPVTARQLEALVRLAEASARMRLSNTVDTEDTDRI
                     LTIVDACLRQVAYDAESGNFDIDKLVTGVTKSQRDIIRSVKEAIRNVSGDSGGQARVD
                     EVIDILLQQGFVRDKIEHTIEQLKRGGEVLEPRHGLVKLIG"
     gene            complement(2024..>2615)
                     /locus_tag="PLV90_10690"
     CDS             complement(2024..>2615)
                     /locus_tag="PLV90_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOF97623.1"
                     /translation="LDEFAAILIFGAASLGLLLAYFAIALTMSTVAKESGNALIYTLV
                     IFAIVSTLLPAFGTMAADVFAGDPPEPPETVVPMPTPAQSSGGGYVTADDPRETDPAW
                     KDYEEARKTYIEKRRLISDISSILSPQANYVMVSLAVTDPGSSTMYTAYGAVQAEPGG
                     LADALGRIWMNIAAIIVFPAAFFAATYVKFMRMDIR"
BASE COUNT          561 a          819 c          798 g          437 t
ORIGIN      
        1 acgagatgga caagatggcg aaggaggacc ggagcgcgct ccacgaggcg atggaacagc
       61 agtgctacga cgacaagact gaggttctca cggagagcgg gtggaagctc ttctgcgatg
      121 tcaccgctga cgaccgcgtc gcgaccctct cctctgaggg cagactcgag tacacagccc
      181 cgtccaactt cgtggcaacg gagtacgacg gagagatgta ctacgtcaaa tcccggcagg
      241 tcgacctcgc ggtcacccca aaccaccgga tgtacgtcaa cctgaaccgg cgtgccgacg
      301 agtgggaggg gttccggctc gtccgcatgg acgagatccc aatccacaag aggatgcggt
      361 tcaagaaagg cgccatatgg gcgggagagc ggcgagatgt ctacgagatc ccgcccgtcg
      421 tcaagttcgc gaaccagaac gccgaaggaa aactcaccga tcccgtcacc gtcgggatga
      481 acgactggct tgagttcctg ggttacttcc tctccgaggg gtcggttcag cggcactcta
      541 agaccggggt gccctaccgc acaatcatat cgcagaagac ctctgagtcg gccgagacga
      601 tccgggagtg cctcgaacgg ctgccgttcc ggttctccta cgacggcaga aacttttcga
      661 tcaatgcaaa acagctcgcc gaacatctcg cgccgttcgg gaaatgccac gagaagtacg
      721 tccccggctg cgcgaaacgt ctacccccgg agcagatcgg gatactcctc gacgcgctca
      781 tgctcgggga tggctacgtc aacaaaacga ccggggtctc gatatacacg acctcttcga
      841 agagactggc cgacgacgtc accgaactcc tgctgaagaa aggatggtcc gggaacgttc
      901 atctcatgcg ggaggccggg acgatcatcg caaacccccg gggcggaacc tcgacgacga
      961 cccacgacat cttccaggtg acgttcatcc gcgacggtca aaacgagccg aacatcaatt
     1021 caaacgggaa gcggcatatc gagaaaaggc cgtataaggg cacgatctac tgccttgaag
     1081 tcccgaacca catcctctat gtccggcgta acggcatccc ggtctggtgc ggcaactcga
     1141 tctcggtcgc caaagccggc atcaccgcga ccctgaagtc ccggtgcgcg cttctcggtg
     1201 cggcgaaccc gaaactcggg cggttcgacc agttcgtccc cataggcgag cagatcgata
     1261 tgccgccctc gctcctctcg cggttcgacc tcatattcgt tatgaccgac cagccggagg
     1321 cccagcgcga cggggcgatc gcgcagcaca tcatcaagac ccatgctgtc ggcgaactga
     1381 tcaagcagca cgagttctcg cccctgccgg gcgtcgacga tgcgtatata gagcgtgccc
     1441 ttgcgccggt caccccagac atcgatccca cgcttctccg gaagtacatc gcctacgcga
     1501 agcggaactg cttccccatc ctctccgacg gggcgaagga ggcgctgata gcttactaca
     1561 tgcgcctgcg aaacctcgcc ggcgggaaca agcccgtccc ggtcactgcc cggcagctcg
     1621 aggcgctggt ccggcttgcg gaggcgagcg cacggatgcg cctctcgaac accgtcgaca
     1681 cggaggacac cgaccgaatt ctcacgatcg tggatgcctg tctccggcag gtcgcctacg
     1741 acgccgagag cgggaatttc gatatcgaca agctcgtgac cggtgtcacg aagtcgcagc
     1801 gcgacatcat ccggtcggtc aaggaggcta tccggaacgt ctccggcgac tccggaggcc
     1861 aggcaagggt cgatgaggtg atcgatatac tgctgcagca gggctttgtc cgcgacaaga
     1921 tcgagcacac catcgagcag ctgaaacggg gcggcgaggt gctcgaaccc cggcacgggc
     1981 tggtgaagct gatcgggtag gctgcaatgc aggcacccga tcttcatctg atatccatcc
     2041 gcatgaactt cacgtaggtc gcggcgaaga aggccgcagg gaagacgatg atcgcggcaa
     2101 tgttcatcca gatccggccg agggcatcgg caagcccgcc gggctccgcc tgcaccgcgc
     2161 cataggccgt gtacatcgtc gaggatccgg gatccgtcac cgccaatgag accatcacgt
     2221 agttcgcctg cggcgatagg atgctggaga tatcgctgat gagccgtcgc ttctcaatat
     2281 aagtctttct cgcctcctcg taatctttcc acgccggatc ggtctcacgg gggtcgtcgg
     2341 cggtgacgta tcccccaccg gaactctgtg ccggggtcgg catcgggacc accgtctccg
     2401 gtggttcggg ggggtcgccg gcgaaaacat ccgcggccat cgtcccgaag gcagggagga
     2461 gcgtggagac gatggcgaag atgacgaggg tgtagatcaa cgcgttcccg ctctcttttg
     2521 ctaccgtcga catcgtcagt gcgatcgcga agtaggcgag caggagcccg agcgaggccg
     2581 ccccgaagat caggatggcg gcgaactcat ccagc
//
LOCUS       DAOSII010000232         2615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_34153_length_2615_cov_43.159766, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000232 DAOSII010000000
VERSION     DAOSII010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34153_length_2615_cov_43.159766"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1293)
                     /locus_tag="PLV90_10695"
     CDS             complement(7..1293)
                     /locus_tag="PLV90_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOF97624.1"
                     /translation="MNGRLSILLLASLIALGSIIAGCTDTSDATSASSAPPTVQTPPD
                     DAGNVTAGNTRFAFDLYRQIASAPAYTGKNIFFSPYSISSALAITYEGARGTTADEIR
                     SVLHLPANETLRRTGFAGIDADLARGDENYTLRTANALWAEKAYPFLPEYIALAERWY
                     GANTTNLDFAKDPEGSRATINRWVERETEDRIRDLLPPGSIDSMTRLVITNAIYFKGT
                     WAMQFDANQTKEEEFRVAPDKTVRIPMMQQTDEDARYPYAETDTLQVLELPYAHGDGA
                     GLSMLVLLPKNDNLTAAEEVLDAGEIAALQGSLVRKRVNVFFPKFRLETEYSLPETLA
                     AMGMPTAFSPDADFSGMDGTKDLFISGVVHKAFVDVNEEGTEAAAATGVVIGLTSARP
                     DSTPIFRADHPFVFLITEKDSGTILFAGRVVNPVGP"
     gene            complement(1478..2545)
                     /locus_tag="PLV90_10700"
     CDS             complement(1478..2545)
                     /locus_tag="PLV90_10700"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOF97625.1"
                     /translation="MLFLSSQESVEWSGHAEEVLRSGTAPGTYDGDIRPNLLSAFDYY
                     IGTVLAARGRAAEGIEWLSAAALGEENDLFSAGFLLGFLERHNGRLAMPSVAFADPRP
                     FMHFAGVPMMREARKRFIAQCGTTLPQFDEPLSMMDIGCGDGGLTVSLLRHLLETGRV
                     AGIGEVLLIDSSPAMVALAEKTVRDAFPDVRIETVNSRIQDCSGSLDHRFDLAVSSLA
                     YHHMPLEQKKIHLARLKPWIDHFILFEIDANNDAPEVYSPELALSVYQSYGSIVDAVF
                     SHDAPVDVAIACVDLFLMAEVISLLTQPRGVRSDYHMLQSQWHALFTECLGPEFAPGC
                     HSPCYADEHTTLFTMHYGREA"
BASE COUNT          485 a          778 c          833 g          519 t
ORIGIN      
        1 cgccccttac ggccccaccg ggtttacgac cctgcctgca aagaggatcg tgccggaatc
       61 tttctccgtg atgaggaaga cgaacgggtg gtccgcccgg aagatgggcg tggaatccgg
      121 gcgtgcactt gtgaggccta tgacgacccc ggttgccgct gccgcctcgg tgccctcttc
      181 gttcacatcc acgaacgcct tatgaacgac gccgctgatg aagagatcct ttgttccgtc
      241 catccccgaa aagtcggcat cgggggagaa cgccgtcggc atccccatcg ccgcaagcgt
      301 ctcagggaga ctgtactccg tctcgagcct gaacttcggg aagaagacgt tgacgcgctt
      361 gcggaccagc gagccctgca gggctgcgat ctctcctgca tccaggactt cctccgcggc
      421 tgtcaggttg tcattcttcg gaagaaggac gagcatcgag agccctgctc cgtctccgtg
      481 ggcatagggg agttcaagca cctggagggt atcggtctcc gcatacggat agcgcgcatc
      541 ttcatccgtc tgctgcatca tcgggatgcg caccgtctta tcgggtgcaa cccggaactc
      601 ctcctccttc gtctggttcg catcgaactg catggcccag gtgcccttga agtagatcgc
      661 gttcgtgatg acgagccggg tcatggagtc gatcgaaccg gggggcagga ggtcgcggat
      721 ccggtcttcg gtctccctct ccacccagcg gttgatggtc gcacgcgatc cctcggggtc
      781 cttcgcgaag tcaaggttcg tcgtgtttgc cccgtaccag cgttccgcga gggcgatgta
      841 ctccgggagg aacgggtatg ccttctcggc ccagagggcg tttgccgtgc ggagggtata
      901 gttctcatcc ccgcgggcga gatcggcgtc gataccggca aatcctgttc tccggagggt
      961 ctcgttcgcc gggaggtgca gcaccgatcg gatctcgtct gcggtcgtgc cgcgggcgcc
     1021 ttcgtaggtg atcgcgaggg ccgaggagat gctgtagggc gagaagaaga tgtttttgcc
     1081 cgtgtaggcc ggggcgcttg caatctgccg gtagaggtcg aacgcgaacc gggtgttccc
     1141 ggccgtcacg ttgccggcgt cgtccggcgg agtctgcacc gtgggtggag cagaactcgc
     1201 cgatgtcgca tccgacgtgt cggtgcaccc ggcgatgatg ctgccgagcg cgatgaggga
     1261 tgcgaggagc aggatgctca gtcgtccgtt catggtctgc gatatggtgc gccgggtaat
     1321 tatatccagc gttggcgacg aattttttcg aagtcttgcg cctttcgcgc ggggtaccct
     1381 gcagcgcctg acagcgatga atcctccgcc ttcgtcaccg gagatgatct cgtaccctac
     1441 gcagcatctt cgtcctttag cagggtatag gtgctgccta cgcttcccgc ccgtaatgca
     1501 tcgtaaagag cgtcgtgtgc tcgtccgcat agcagggcga gtgacagccc ggggcgaact
     1561 ccggacccag gcattccgtg aagagcgcat gccactggct ctgcagcata tgataatcgc
     1621 tcctcacacc gcggggctgt gtgaggagcg agatgacctc cgccatcagg aagaggtcta
     1681 cgcaggcgat ggccacgtcc accggggcat cgtgcgagaa gacggcatcg acgatactcc
     1741 cgtaagactg gtagaccgag agcgcaagct ccggggaata cacctccggg gcatcgttgt
     1801 tcgcatctat ctcaaagagg atgaagtggt cgatccaggg tttgagccgc gcgaggtgga
     1861 tcttcttctg ctcaagcggc atgtggtggt aggcaagcga gctgacggcg agatcgaaac
     1921 ggtgatcgag gctgccggaa cagtcctgga tccggctgtt cacggtctcg atgcggacat
     1981 cgggaaatgc gtcgcggacg gtcttttccg caagggcgac cattgcaggc gacgagtcga
     2041 tgaggaggac ctcaccgatc ccggcgaccc tgccggtctc taacaggtgt cgcaacagcg
     2101 atacggtgag ccctccatcg ccgcacccga tatccatcat ggagagcggt tcatcgaact
     2161 gcggcagggt ggtgccgcac tgtgcaatga accgctttcg tgcctcccgc atcatgggga
     2221 caccggcaaa gtgcatgaac gggcgcgggt cggcaaacgc cacagagggc atggcaagtc
     2281 tcccgttgtg gcgctcgaga aagccaagga ggaatcccgc agagaagaga tcgttctcct
     2341 ctccaagagc agcggcgctc agccattcga tcccctctgc ggcccgtccg cgggcggcaa
     2401 gcaccgttcc tatatagtag tcgaaggcgg agagcagatt cggccggatg tcgccgtcat
     2461 aggtgcccgg cgcagtcccg cttctgagaa cctcctctgc atgaccggac cactccaccg
     2521 actcctgcga tgaaagaaag agcataacga ttatattctg ccggcaaccg ctatgaggat
     2581 tcgtattctc tccgccctta cgatccgggg gatca
//
LOCUS       DAOSII010000233         2570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_34938_length_2570_cov_46.724453, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000233 DAOSII010000000
VERSION     DAOSII010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34938_length_2570_cov_46.724453"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PLV90_10705"
     CDS             <1..296
                     /locus_tag="PLV90_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868422.1"
                     /note="UbiA prenyltransferase family catalyzes the
                     transfer of a prenyl group to various acceptors with
                     hydrophobic ring structures in the biosynthesis of
                     respiratory quinones, hemes, chlorophylls, vitamin E, and
                     shikonin; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOF97626.1"
                     /translation="ANDRARGMATIPVLYGMRGAAAWVAGCAAAHAVTAVAFGLALGP
                     VALAGFLAGLALLAGAACLILRGKSPAAAMQALPLIHATLIVYAVAIIATVAF"
     gene            complement(303..626)
                     /locus_tag="PLV90_10710"
     CDS             complement(303..626)
                     /locus_tag="PLV90_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HOF97627.1"
                     /translation="MTPETATAEAAKSVTIDIVSVNMAFDLDTITVPAGAEVTINLDN
                     QDVGIPHNVSFYTDSSAAETIFVGDLITGPDKVTQTFTAPAEPGSYYFQCDVHPFMNG
                     TFVVE"
     gene            complement(675..>2570)
                     /locus_tag="PLV90_10715"
     CDS             complement(675..>2570)
                     /locus_tag="PLV90_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HOF97628.1"
                     /translation="YPASLLLFALATWYCGLLHIPVALALLIFIALGVYGLYRGDYRG
                     LRGFLAWDAVFLVGFLFALSVRFANPVIGSFSEQYMNHAFLASIIREPIVPPLDPWFA
                     GGHLTVYYYLGHWLMGCLAVVTGTPSRIAFNLIPATVYGTAFVALYALGHLFLRRLRW
                     VPLAVLLLVPPSVLWFLAAGGDIYSAFQETNWIISGARFEFPAFSLFLGNAHAFEMAV
                     FNQIFLIFLLGFAWLRWGGLDTRGRWGLALLIALSLGSMPLLSSWDALVYGPAAAVFL
                     LALLLRERDRSTVAAAVAVPAIALLIYIPYYLQLEPAGVGGIGMGFPPTDPLAFIAIW
                     GGFLAVIYIAVARDIRQSPLYLGVALPFLVAGYAALGIVAVPLAYLIRRRQRSFADIL
                     SLAGLLVLALCDVFYIKEMLGGDYSRFNTIFKFYFDAWILIGTGLLLTIGGWFAARRP
                     ILPARRWVAVAAAAALVAAPLALDIDIGRGLLGITHPPEGYHTLDGLASIDPGEAAAI
                     DYLGTLGGGHRLVEAENGDYGYYSRVSSFSGIPAVLGQISHELTWRGNGAWYTDRPAD
                     IRAIYENPGETLALMAKYNATLLYVGEAERARYDVCLPGSGLVLIYEGDGVRIYAPEG
                     VTIIPPG"
BASE COUNT          506 a          781 c          928 g          355 t
ORIGIN      
        1 tcgcaaacga ccgcgcccgg gggatggcga cgatcccggt cctctacggc atgagaggtg
       61 cggccgcctg ggtcgccggg tgtgcggccg cccatgccgt gacggcggtc gccttcggcc
      121 tcgccctcgg accggtggcc ctcgccggat tcctggcggg gctcgccctc cttgcaggcg
      181 cagcctgcct catcctccgg gggaaaagcc cggctgccgc gatgcaggcg ctcccgctca
      241 tccacgcgac cctgatcgtc tacgctgtag cgatcatcgc gaccgtcgcg ttctgatgga
      301 tcctactcca cgacgaacgt cccgttcatg aacgggtgga catcgcactg gaagtagtag
      361 gaccccggct ccgccggcgc cgtgaatgtc tgggtcacct tgtccgggcc ggtgatgagg
      421 tcgccgacga agatcgtctc ggccgccgat gagtcggtgt agaacgagac gttgtgcggt
      481 atgccgacat cctggttgtc gaggtttatg gtcacctccg cacccgccgg gaccgtgatc
      541 gtgtccaggt cgaacgccat gttcacggat acgatatcga tggtcacgct cttcgcggcc
      601 tcggccgtag ccgtctccgg ggtcacgccc gcacctccat gactgcagtt gctcgcgtca
      661 tgagccccac cctgtcaccc gggaggtata atggttacgc cttccggtgc gtatatccgg
      721 accccgtccc cctcgtagat caggacgagc ccgctccccg gcagacaaac gtcgtaacgc
      781 gcccgttccg cctcaccgac gtagaggagg gtcgcattgt acttcgccat cagggcgagg
      841 gtttctcctg ggttctcgta gatcgcccgg atgtccgccg ggcggtcggt ataccacgcc
      901 ccgttccccc gccaggtcag ctcgtggctg atctggccga ggactgccgg gatgccggag
      961 aacgacgaga cacgggagta gtagccgtaa tccccgttct ccgcctccac gagccggtgg
     1021 ccgccgccga gcgttccgag gtagtcgatc gccgcggcct ccccggggtc gatggaggcg
     1081 agcccgtcga gggtgtggta gccttccggg ggatgggtga tcccgaggag cccccggccg
     1141 atgtcgatat cgagagcgag cggggcggcg acgagcgccg cggccgcggc gacggcaacc
     1201 caccgccgcg ccgggaggat cgggcggcgg gcggcaaacc atccccctat ggtgaggagc
     1261 aagccggtcc cgatgaggat ccaggcatcg aagtagaact tgaatatggt gttgaaccgg
     1321 ctgtagtcgc cgccgagcat ctccttgatg tagaagacat cgcagagggc gagcaccaga
     1381 agcccggcaa gagagaggat gtcggcaaac gagcgttgac gccggcggat gaggtaggcg
     1441 agcggcaccg cgacgatacc gagggcggcg tagccggcga cgaggaacgg gagagcgaca
     1501 ccaagataga gcggggactg ccgtatatcg cgggcaacgg cgatgtagat caccgcgagg
     1561 aacccgcccc agatcgcgat gaacgcgaga ggatcggtgg ggggaaaccc catgccgata
     1621 ccgcccacgc ccgcgggctc aagctgcagg tagtagggga tgtagatgag gagagcgatc
     1681 gccgggaccg cgacggcggc ggctacggtg gaacggtccc gctcccggag taaaagggca
     1741 aggaggaaga ccgcagccgc cgggccgtag acgagggcat cccaggagga gaggagcggc
     1801 atcgagccga gggagagggc gatgaggagg gcaagacccc accgcccccg ggtatcgagc
     1861 ccaccccacc ggagccacgc aaacccgagg aggaatatga ggaagatctg gttgaagacg
     1921 gccatctcga aggcgtgggc gttcccgagg aagagggaga aggccgggaa ctcaaaccgg
     1981 gcgccggata tgatccagtt ggtctcctgg aacgcggagt agatatcccc gcccgcggca
     2041 agaaaccaga gaaccgacgg cgggacgagg aggaggaccg cgagcggcac ccaccgcagc
     2101 cgccggagga agaggtgccc gagggcgtag agcgcgacga acgccgtccc gtagaccgtg
     2161 gccgggatga ggttgaaggc gatccgggag ggggtgccgg tgactaccgc gaggcagccc
     2221 atcagccagt ggccgaggta gtagtagacg gtgaggtgcc cgccggcaaa ccaggggtcg
     2281 agcggcggca cgatgggttc gcggatgatg ctcgcaagga aggcatggtt catgtactgc
     2341 tcgctgaaac tgccgatgac cgggtttgcg aaccggacag agagagcaaa gagaaagccg
     2401 accaggaaga ccgcatccca ggcgaggaac ccccggagtc cccggtaatc gccccggtaa
     2461 aggccgtaga caccgagggc gataaagatg aggagggcga gggcgaccgg gatatggagg
     2521 aggccgcagt accaggtcgc gagggcgaag aggaggaggg aggccgggta
//
LOCUS       DAOSII010000234         2530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_35638_length_2530_cov_40.033535, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000234 DAOSII010000000
VERSION     DAOSII010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35638_length_2530_cov_40.033535"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="PLV90_10720"
     CDS             <1..621
                     /locus_tag="PLV90_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOF97629.1"
                     /translation="KTAAFRKVFSFAPPAHLDEIIAFHLENGGMSRYDKFRYIYAHIL
                     HEPLTPKQEERLASKYVSLIFESMLTVPCVEGAEDLLHDCSRQLPLYIVSATPEGEMQ
                     EIARRRGLTKYFVRVYGSPKTKAECIREILAETGASPEEALFVGDAPNDWEAAHETGV
                     RFVARIPPGDPDRFVGRPGVEGIVANLHELREYLRESVCSSRSHTP"
     gene            594..1262
                     /locus_tag="PLV90_10725"
     CDS             594..1262
                     /locus_tag="PLV90_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOF97630.1"
                     /translation="MLIPISYPLNRATPLYPGTEPLTITAIKSFEKGDAEEKSLITAS
                     SHAGTHIDLPRHFCPGGSSVRSLLAPEAVFAPARCIDVPKAGVEPIRIYDLLPHLDAI
                     RTSRALFIRTGSGRLRESNPDAYATRHPWVHPEVPEFLCREGPAIRLFGIDTISIAVP
                     AKPEEGQKAHCAFLCRSPPIFLLEDVDLSYGRLLEEPWTLRVYPMVFDDLEAVPVVAL
                     AEFG"
     gene            complement(1312..1740)
                     /locus_tag="PLV90_10730"
     CDS             complement(1312..1740)
                     /locus_tag="PLV90_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOF97631.1"
                     /translation="MGEVEGVDKGRTIDQLRRCLAGREGVLLGYLYDSFLRHGRFHDI
                     DIGLLVSGKREPYESYKYAMRIASDLERCITPRYEIDLRILNDAPVEFQYEVVRTGHL
                     LVARDEATRVDFEAGVIAQFLDLKYLYDRIDRALLARVGE"
     gene            complement(1907..2167)
                     /locus_tag="PLV90_10735"
     CDS             complement(1907..2167)
                     /locus_tag="PLV90_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97632.1"
                     /translation="MELGFDREKNRQERLAFVREYALWVRRVPNEVWSREQADLIDAF
                     LANAGNYALSRARYLEMQRDIAERRRPVPVDEHGCARSTETS"
     gene            complement(2151..>2530)
                     /locus_tag="PLV90_10740"
     CDS             complement(2151..>2530)
                     /locus_tag="PLV90_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97633.1"
                     /translation="ELLARGFYFLNSDDIGEIYSMLRDRLAVRIAEGERIIPNIEMKF
                     KKDDFDRYAMAMARTVRFDDIRFCISPIELQIPYKLYLASDKDIEDAVYLWVLFRDML
                     DGDLMRSFMERLRVRGEPYGIGV"
BASE COUNT          508 a          836 c          704 g          482 t
ORIGIN      
        1 aagaccgccg cattccgcaa agtcttctcc ttcgcacccc cggctcacct cgacgagatc
       61 atcgccttcc acctcgagaa cggcgggatg tcccggtacg acaagttccg ctacatctac
      121 gcccacatcc tccacgagcc cctcaccccc aagcaggaag aacgccttgc aagcaagtac
      181 gtcagcctga tctttgaatc catgctcacc gtcccctgtg ttgagggcgc ggaggacctc
      241 ctccacgact gctcccggca acttccgctc tacatcgtct ccgcgacccc ggagggcgag
      301 atgcaggaga ttgcccgccg gcggggcctc acgaagtact tcgtccgggt atacggctcg
      361 ccgaagacga aggccgagtg catcagggag atcctcgccg agaccggcgc gtccccggag
      421 gaggccctct tcgtcggcga tgccccgaac gactgggagg cggctcacga gaccggcgtc
      481 aggttcgtcg cacggatccc gcccggcgac ccggaccggt ttgtcggccg gccgggggtg
      541 gaggggatcg tggcgaacct tcatgaactc cgggagtacc tacgggagag cgtatgctca
      601 tcccgatctc atacccctta aaccgggcaa cgccgctcta ccccggaacg gaaccgctca
      661 cgatcaccgc gataaagtcc tttgagaagg gcgatgcgga agaaaagagc ctgatcaccg
      721 catcaagcca cgccgggacg cacatcgacc ttccccggca cttctgtccg ggcgggagct
      781 cggtcaggag cctccttgcg ccggaggcgg tctttgcacc ggcgcgttgc atcgatgtgc
      841 cgaaggccgg ggtggaaccg atccggatct acgacctcct tccacacctc gacgcgatcc
      901 ggacctcgcg cgcgctcttc atccggaccg ggagcggtcg gctccgggag agcaaccccg
      961 atgcctacgc aacgaggcac ccctgggtcc acccggaagt gccggagttc ctctgcaggg
     1021 aggggccggc catcaggctg ttcgggatcg ataccatatc catcgccgtc ccggcaaagc
     1081 cggaggaggg gcaaaaagct cactgcgcgt tcctctgtcg gtccccgccg atcttcctcc
     1141 ttgaggacgt ggatctctcc tacggccggc tgctcgagga gccctggacc ctgcgggtct
     1201 acccgatggt cttcgatgac ctggaggcgg tgccggtggt ggcgcttgcg gagtttggat
     1261 gataccgctc cctcagggcc tcatccagct cccgcatatg gtaaaagact ctcattctcc
     1321 cacccgggca aggagagccc tatcgatccg gtcatagaga tatttcaggt cgaggaactg
     1381 tgctatcacg ccggcctcga aatcgacgcg ggtagcttca tcccgggcga caaggaggtg
     1441 accggtcctc accacctcat actggaactc cacgggcgca tcgttcagga tgcggagatc
     1501 gatctcatat ctcggcgtga tacaccgttc aagatcggat gctatcctca tagcatattt
     1561 gtaggattca tagggttctc tctttccgga gacaagcagc cctatatcga tatcgtggaa
     1621 cctgccatgg cgaaggaacg aatcgtagag atatccaaga agcacaccct ctctgccggc
     1681 gagacaccgg cggagttgat cgatggtccg ccctttatca acaccttcca cttctcccac
     1741 ctcccgcaac aacagaccct cgcaagggct cacccttacg tatattcggg cccatcggca
     1801 aagagggttg ctctgcccat caaccctccc ctggagcagc tcccggcatc ctgctgccgg
     1861 aggatccggg agccctgccc gggctcaaat ctcatgaaga gtaccgttac gaagtctccg
     1921 tagatcgcgc gcacccgtgc tcgtccaccg ggacggggcg ccggcgctcc gcgatatccc
     1981 gctgcatctc aagatatcgg gcgcgggaga gagcgtaatt tccggcgttt gcaaggaacg
     2041 catcgatcag gtcggcctgc tcgcggctcc agacttcgtt aggaacccgc ctgacccaga
     2101 gagcgtactc cctgacaaag gccagcctct cctgccggtt cttctcccgg tcaaacccca
     2161 attccatagg gttccccccg gacacgcagg cgctccatga acgatcgcat gagatctcca
     2221 tccagcatat cgcggaacaa cacccagaga tagacggcat cctcgatatc cttgtcgctt
     2281 gcaaggtaga gtttataggg gatctgtagt tcgatgggcg atatacaaaa ccggatatca
     2341 tcaaacctta cggtccttgc catggccatt gcgtaacgat cgaagtcgtc cttcttgaac
     2401 ttcatctcga tgttcgggat gatcctctct ccctctgcaa tccgcacggc aagcctgtcg
     2461 cggagcatcg agtagatctc gccgatgtcg tccgagttca ggaagtagaa tcctcgtgca
     2521 agaagttcat
//
LOCUS       DAOSII010000235         2512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_35964_length_2512_cov_37.846968, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000235 DAOSII010000000
VERSION     DAOSII010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35964_length_2512_cov_37.846968"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..1469)
                     /locus_tag="PLV90_10745"
     CDS             complement(69..1469)
                     /locus_tag="PLV90_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOF97634.1"
                     /translation="MKPTAAPDLPPGLLWFNTDRPLSLSDLAGRVVLLSFRTFACANC
                     MRLVPEIRRLRKEHPALVVIGVHAPGLEPAAVAGNFAEAVRRSGAGYPVAIDRDHRLR
                     EAFGIRTWPTFVLIDPEGKIVGTTTGEGIAGRLGPTIGRLGGDSAGPLQAGGVSVVKG
                     ALYYPGKVAADHAGMRLFISDTGHNRIVVAGTDGRIIETIGSGEPGNADGSSGKAAFY
                     RPEGVAFDEAAGILYVADTGNHMIRAVSWPDRAVATVAGTGLSVPGALNAPRDITLLG
                     GFLYIAGARRIWRMNLATRETEPYAGSGREALVDGPLMEAAFAGPSGIVTDGEALYVA
                     DREASAIRRIKRGMVETRIGHSLEDFGDLDTIAGMARIHHPAGLAYHQGLIAIADTGN
                     HKIKEFDPETGWVLTRAGSGGQGDRDGLSGDATFNEPGGLVNLGGLWYIADTKNHVVR
                     VYDPAGHVVSTLRLWG"
     gene            complement(1494..2390)
                     /locus_tag="PLV90_10750"
     CDS             complement(1494..2390)
                     /locus_tag="PLV90_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97635.1"
                     /translation="MPGADYIYPNVTEMAQRGGLLERWRLAEPLGCTYVEVPADLVKN
                     RTEVERTGQDIGSILKRSSVELLYEQEAGLPGNLAYLLHTEPAIPRRDGHGRQVKADL
                     RWRDRAWLATFGDMLLDIEEYIGIPPDIIEIHPGSRKNTYADIVAAMYSLIVAHWNAF
                     GVEPLVLLENHADQSVATAGQMEAFWTTLKELHPEIAGLAGIALDPWHLRAAASDEFP
                     GALRRVPPAALKAFHIHNNLVPPTAADRMPWQEVFSVVRSLPARPRIKPVVYQESRVA
                     GAIDFCEGMLAQPRAVLPGQHS"
BASE COUNT          382 a          883 c          849 g          398 t
ORIGIN      
        1 ggccgcggcc cgggaggggg gtgcgggcgg tcttcccctg gcggctgcag ctcgccagca
       61 aacccgccct acccccagag cctcagggtc gagaccacgt ggccggccgg atcgtagacc
      121 ctgacgacgt gatttttggt atcggctatg taccagagcc ctccgaggtt gaccaggccg
      181 ccgggctcgt tgaacgtcgc gtcccccgag agcccgtcgc ggtcaccctg ccccccgctc
      241 ccggcccggg tgaggaccca gcccgtctcc gggtcgaact ccttgatctt gtggttgccg
      301 gtatcggcga tggcgatcag cccctggtgg taggccaggc ccgcggggtg gtggatacgc
      361 gccataccgg cgatggtgtc gaggtcgccg aagtcctcaa gtgaatgccc gatccgcgtc
      421 tcgaccatcc cccgcttgat ccggcggatc gccgacgcct ccctgtcggc cacgtagagc
      481 gcctcgccgt cggtgacgat ccccgacggg ccggcaaacg cggcctccat gagcggccca
      541 tccaccaggg cctcccggcc cgacccggcg tacggctccg tctcgcgggt ggcaaggttc
      601 atccgccaga tccggcgtgc ccccgctatg tagaggaacc ccccaagcag cgtaatgtcc
      661 cgcggcgcgt tcagcgcccc cgggacggag agccccgtcc ccgcgaccgt cgcgaccgcc
      721 cggtcgggcc aggataccgc ccggatcatg tggttcccgg tgtccgcgac gtagaggatg
      781 ccggccgcct cgtcgaaggc gacaccctcc ggccggtaga acgcggcctt gccagacgat
      841 ccgtcggcgt tccccggctc cccggaccct atcgtctcga tgatccgccc gtcggtgccg
      901 gcgacgacga tccggttgtg gccggtatcg ctgatgaaca accgcatacc cgcgtggtcc
      961 gccgctacct tcccggggta gtagagggcg ccctttacaa cggatacgcc ccccgcctgc
     1021 agcggcccgg cggagtcacc gccaagccgg ccgatcgtcg ggccgagccg cccggctatc
     1081 ccctcgccgg tcgtcgtccc gacgatcttc ccctccggat cgatgaggac gaacgtcggc
     1141 caggtcctga tgccgaaggc ctcccggagc cggtgatcgc ggtcgatagc gaccggatac
     1201 cccgccccgg agcgccggac cgcctcggcg aagttcccgg cgaccgcggc gggctctaac
     1261 ccgggcgcgt ggaccccgat gaccacgaga gcggggtgct cctttcggag acgccggatc
     1321 tccggcacaa ggcgcatgca gtttgcgcag gcaaacgtcc gaaacgagag cagcacgacc
     1381 ctgcccgcga ggtcggagag ggagaggggg cggtcggtgt tgaaccagag aaggcccggg
     1441 gggagatcgg gcgcagccgt cggtttcatg cggcaccctc tggcatcgag aagtcatgaa
     1501 tgttggcccg gcagcaccgc ccgcggttgt gcgagcatac cttcgcagaa gtcgatcgct
     1561 ccggcgaccc ggctctcctg gtagacaacg ggtttgatac ggggccgtgc cggcagactc
     1621 cggaccaccg agaagacctc ctgccagggc atccggtcgg ccgccgtcgg cggtacaaga
     1681 ttgttgtgga tgtggaaggc cttgagggcc gcgggcggga cccggcgaag cgccccggga
     1741 aactcatcgc tcgcggcggc gcggaggtgc caggggtcga gggcgatacc ggcaagaccg
     1801 gcgatctccg ggtgaagttc cttgagcgtc gtccagaacg cctccatctg gcccgctgtc
     1861 gccacgctct ggtccgcgtg gttctcaagc agcaccaggg gctccacgcc gaaggcgttc
     1921 cagtgggcga cgatcaggga gtacatcgcc gcgacgatat cggcgtaggt gttcttccgg
     1981 ctgccgggat ggatctcgat gatgtccggg ggaataccga tatattcctc gatatcgagg
     2041 agcatatccc cgaacgttgc cagccaggcg cggtcgcgcc accgcaggtc cgccttcacc
     2101 tgccgcccgt gcccgtccct ccgggggatg gcgggctcgg tgtgcaggag atacgcgagg
     2161 ttgcccggga ggcccgcctc ctgctcgtaa aggagctcga cggacgacct cttgagtatg
     2221 gagccgatat cctgcccggt ccgctccacc tcggtcctgt tcttgacaag gtcggcaggg
     2281 acctcgacgt acgtgcaccc gagcggttcg gcgaggcgcc agcgttcgag cagccctccc
     2341 cgctgcgcca tctcggtgac gtttgggtag atgtagtctg cgcccggcat cgtgatcggg
     2401 ctaccccatg gcggaagggc tacaaatatg ttgggggcgg gcggtaccgc ctccccggca
     2461 ggccgtcaac ccgacctatt cttctcggtg cccccatgca cccggcccgc gg
//
LOCUS       DAOSII010000236         2504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_36112_length_2504_cov_45.547979, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000236 DAOSII010000000
VERSION     DAOSII010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36112_length_2504_cov_45.547979"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="PLV90_10755"
     CDS             complement(<1..725)
                     /locus_tag="PLV90_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOF97636.1"
                     /translation="MDYRTVTALLVIVTVLLLSAAGCTATTEENVPGAASISPADEAY
                     ARGVAAYADASYRTAEEYFAEAYALSTGAGDPDRARIARDAMFRANRTYTEYPFNRSA
                     AEAALAETIPGITDAAIADWLDNRAQAIVSENETLYFSDVANNYLYAHFDEIRKITAT
                     SLDFDYVARYAWSEDQPEGTGPYVNPVRYAGAERLEVPREMLPSSGVLKIWFPLPVET
                     DSQRNVTVTNLSCPDFIVAGPFTT"
     gene            1209..1775
                     /locus_tag="PLV90_10760"
     CDS             1209..1775
                     /locus_tag="PLV90_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97637.1"
                     /translation="MNGAQVYFGNDYKGTIEAGVLYVPVYTTGTPYQTFRVEKEGYTT
                     HYGDVISVPGKGETFDLYATLNPIQPTQEPLIGGDIGWYTVHSNVDGATVQFDNDVKG
                     TISKGILTVQVYTTGTPYRTYTLTKDGYTTYTGSIDRYPGKGETVDLYATMNPAPTPA
                     PKSPLSPALAGSALLIAGLLLIAGRKNN"
     gene            complement(1906..>2504)
                     /locus_tag="PLV90_10765"
     CDS             complement(1906..>2504)
                     /locus_tag="PLV90_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOF97638.1"
                     /translation="TIIPNFINASMPDLFIVIFMLALLAAAMSTLSSLFHVMGTSLGF
                     DVMRRTGSKGASMRLIQVATVVMIVASVVIAFIMPGSIIARATAMFMGLCASAFLPAY
                     AHAMYSSRPSLFAAKVSLIIGAVVWFLWTAFVHIKESATLGLSNLIFGVPAILPAPWQ
                     DVDPLVIALPLSLAALMVGLVLDRHRVSVEEEGPTGAA"
BASE COUNT          521 a          742 c          761 g          480 t
ORIGIN      
        1 gtggtgaacg ggccggcgac gatgaagtcc gggcacgaga ggttcgtgac ggtgacgttt
       61 cgttgcgaat ccgtctcgac cgggagcggg aaccagatct tcaggacccc ggacgagggg
      121 agcatctcgc gaggaacttc cagacgctcg gcccctgcgt agcgtaccgg attcacgtag
      181 ggtccggtcc cttcgggctg atcttccgac caggcatacc tggcaacgta gtcgaagtcg
      241 aggctcgtcg cggttatctt ccgtatctca tcgaaatgcg cataaagata gttgttggcg
      301 acgtcggaga agtagagcgt ctcattctcc gagacgatcg cctgggcgcg attatccagc
      361 caatcggcta tcgccgcatc ggtgatgccg gggattgtct ccgcgagtgc cgcctctgca
      421 gccgatctgt tgaacgggta ctccgtatac gtcctgttcg cccggaacat ggcgtctcgt
      481 gctatccgcg ccctgtcagg gtcgccggcg ccggtggaga gggcatacgc ctcggcaaaa
      541 tattcttcgg cagtccggta gctggcgtcc gcgtatgcgg ccaccccccg ggcgtaggcc
      601 tcgtcggccg gcgagataga ggccgcgccg ggtacgttct cttctgttgt ggcggtgcat
      661 cccgctgcag agaggagaag gacggtaact ataaccagaa gtgcagtgac tgtgcggtag
      721 tccataaaga gaagaacatg tgagtgctga tgaacctttc tctttttttc ggcggctttc
      781 acccggcttt tggggatctt gtcgcgatat cttcacaata atccctccag cgccgactga
      841 ctgccgcttt cagatcgcgc cgggcgaagc ggccgctccc cggccctctg ccctggtgag
      901 cgcgacgggg cgcccttcca ccttgagggc agatccgcgc ctccgactga aggccgggat
      961 catcgcgcat ggacgggatt acgctggaat tatcggttaa atcatacttc aggcaggata
     1021 tgatccggca cagaatagaa gagtatatcc gtgataagat gaatcacttg attgaagcgg
     1081 aggtttctat gcagagcatt ctacgttatc ttttagcggt tttccttgtt gcctgctgtt
     1141 tcgtccttcc tgcaagcgct cagccgccta taggcgggga caacggatgg tatgcggtca
     1201 actgtaacgt gaacggagca caggtctatt tcggcaatga ttataagggc acgatcgagg
     1261 ccggcgtact ctacgtcccg gtctacacca cgggaacccc ttaccagacg ttccgcgtcg
     1321 aaaaggaggg ttacacgacc cactacgggg atgtaatctc ggtcccgggc aagggcgaga
     1381 cgtttgacct ctacgcgacc ttgaacccga tccagcccac gcaggagccg ctgatcgggg
     1441 gagatatcgg ctggtacacc gtccactcca acgtcgacgg cgcgacggtc cagttcgata
     1501 acgatgtcaa gggcacgatc agcaagggta tcctcacggt gcaggtctac acgaccggga
     1561 cgccgtacag gacctatacc ctgacaaagg acgggtatac gacttacacc ggttcgatcg
     1621 accgctaccc cggcaagggc gagacggtcg acctgtacgc gacgatgaac ccggcgccga
     1681 cccccgcccc gaaatcgccg ctctccccgg cccttgccgg cagcgccctg ctgatagcag
     1741 gactgctgct gatcgcaggg agaaagaaca actaacctct cactcttttt tgcggccgct
     1801 ctaccctctg ctcggggatg gagcctcgtt acctgccgga tatccgcaga gcacgacgta
     1861 tatccccctg ctcccgccca aaaaaggttt gcgagaaagg aaccctcagg cagccccggt
     1921 cggtccctcc tcctctacgg acacccggtg gcggtcgagc accagcccga ccatcaacgc
     1981 ggccagagag agagggaggg cgatgacgag agggtccaca tcctgccagg gtgcaggaag
     2041 aatcgccggc acaccgaaga tgaggttgga gagcccgagc gttgccgact ccttgatgtg
     2101 gacaaatgcg gtccagagga accagaccac ggctccgatg atcaggctca cctttgcggc
     2161 gaagagcgac ggcctgctgc tgtacatcgc atgggcgtat gccggcagga acgccgatgc
     2221 gcagaggccc atgaacatcg ccgtcgcccg cgcgatgatg ctgccgggca tgatgaaggc
     2281 gatgacgacg ctcgcgacga tcatgacgac ggtcgccacc tggatcagcc tcatcgatgc
     2341 tcctttagaa ccggtgcgcc gcataacgtc aaacccaagc gacgtcccca tgacatggaa
     2401 gagagaactg agcgtggaca tcgcggcggc aaggagcgcc agcatgaata tgacgatgaa
     2461 gaggtccggc atcgacgcgt tgatgaagtt cggtatgatc gtgt
//
LOCUS       DAOSII010000237         2482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_36539_length_2482_cov_44.683148, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000237 DAOSII010000000
VERSION     DAOSII010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36539_length_2482_cov_44.683148"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /gene="wecB"
                     /locus_tag="PLV90_10770"
     CDS             complement(<1..952)
                     /gene="wecB"
                     /locus_tag="PLV90_10770"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOF97639.1"
                     /translation="MKIVSIVGARPQFIKCAPVSRELRKEHEEILVHTGQHYDHGMSA
                     VFFEELAIPEPDYNLGIGSGTHGRQTGAMLGAIEDVLEKEEPEIVLVYGDTNSTLAGA
                     LAAAKMHVPVAHVEAGLRSFDRRMPEEVNRVLTDHASDILFCPTATAVRNLATEGVTE
                     GVFLVGDVMVDAMNHNRAIAEERSRILEDAGVRPGEYLVVTVHRPSNTDSRENMAAII
                     GALGEAGVPVVFPVHPRTRKFLLGYDLLAAMPENIRITEPLGYLDMLRLMAHAKKILT
                     DSGGVQKEAYMLGVPCITLRENTEWVETVEAGWNVLVGAER"
     gene            complement(955..2391)
                     /locus_tag="PLV90_10775"
     CDS             complement(955..2391)
                     /locus_tag="PLV90_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOF97640.1"
                     /translation="MSNTLQSIVDARGPIRKIGVVGMGYVGIPAAVLFADAPEFESVL
                     GFQRASASSGYKIEMLNRGESPLKGEEPGLEELLGKVVNAGKFRCTSDFSEIAGCDAV
                     TLAIQTPFLDPKDLIPDFSALNEGLRAVGRHLSEGTLVVLESTVTPGTTAGMAREILE
                     SESGLVAGEDFCLAHAPERVMVGRLLRNIREHDRIVGGIDDVSTARAVELYRPVLTTG
                     EIIPMTATAAEVTKTAENAFRDLQIAAANQLALHCEAMGVNVYDVRAGIDSLKGEGIT
                     RAILWPGAGVGGHCLTKDSWHLERGAQVLGRGDLWYPHGAESIFGVARKLNEFMPRHM
                     VHLTIEGLRRVGKSTEGTTIALLGWAFIQNSDDTRNTPAEPYLAAMEEAGAAVRVHDP
                     FVDEYPEIEVSHDLAGTLAGADVVVVFTAHHHYTALDPARVKELSGREHPVIVDGRNV
                     VDPDAFIRAGFVYKGIGRGDKNSHPVRR"
BASE COUNT          416 a          869 c          767 g          430 t
ORIGIN      
        1 cccgctccgc ccccacgagc acgttccacc cggcttcgac cgtctcgacc cactcggtgt
       61 tctcccggag ggtgatgcag ggcacgccga gcatgtatgc ctccttctgg accccgcccg
      121 agtcggtgag gatcttcttc gcatgcgcca tcaggcggag catatcgagg tagccgagcg
      181 gttcggtgat ccggatgttc tccggcatcg ccgcgaggag atcgtatccc agaaggaact
      241 tccgcgtccg ggggtggacc gggaagacca ccggcacccc ggcctccccg agggcgccga
      301 tgatggcggc catgttctcc cggctatcgg tattcgacgg ccgatgaacg gtgacgacga
      361 ggtactcccc cggccggacc ccggcatcct cgaggatccg ggagcgctcc tccgcaatcg
      421 ctcggttgtg gttcatcgcg tcgaccatca cgtccccgac gaggaagacg ccctccgtga
      481 ccccttcggt ggcaaggttt cgcacagccg tcgccgtcgg gcagaagagg atgtccgatg
      541 cgtgatccgt aagcacccgg ttcacctctt cgggcatccg gcgatcgaaa ctccggagcc
      601 ccgcctcgac gtgcgccacc ggcacgtgca tcttcgccgc cgcaagggcg ccggcaagcg
      661 tcgagttcgt gtccccgtag actaaaacga tctcgggctc ctccttctcg aggacatcct
      721 cgatcgcccc gagcatcgcc ccggtctggc ggccgtgcgt ccccgagccg atgccgaggt
      781 tgtagtcggg ctccgggata gcgagctcct cgaagaagac cgcagacatt ccgtggtcgt
      841 aatgctggcc cgtgtgaacg aggatctcct cgtgctcctt ccggagctcg cgcgagacgg
      901 gagcgcactt gatgaactgc ggccgggcgc cgacgatcga tacgatcttc atggttaccg
      961 ccggaccggg tggctgtttt tatcgccccg accgatgccc ttgtagacga accccgcccg
     1021 gatgaacgca tccgggtcga cgacgtttct gccgtcgacg atgaccgggt gctcccgtcc
     1081 cgagagctcc ttcacccgcg ccgggtcgag agcggtatag tggtgatgcg ccgtgaagac
     1141 gacgacgaca tccgcccccg cgagcgttcc cgcaaggtcg tgcgagacct cgatctcagg
     1201 atactcatcc acaaacggat cgtggaccct gaccgccgca cccgcttcct ccatcgccgc
     1261 gaggtaaggc tccgccggcg tgttcctggt gtcgtccgag ttctggatga acgcccaccc
     1321 gaggagcgcg atcgtcgtgc cctccgtgga cttccctacc cgcctgagcc cttcgatggt
     1381 caggtggacc atgtgccggg gcatgaactc gttgagcttg cgcgcgacgc cgaagatcga
     1441 ctccgccccg tgcgggtacc agaggtcgcc acgcccgagg acctgcgcgc cacgctcgag
     1501 gtgccaggaa tccttcgtga ggcagtggcc gccgaccccg gcacccggcc agagaatcgc
     1561 ccgggtgatc ccctcgccct tcaaggagtc gatccccgcc cggacgtcgt agacattgac
     1621 gcccatcgcc tcgcagtgca gtgcgagctg attcgcggcc gcgatctgga ggtcgcggaa
     1681 ggcgttctcg gcggtcttcg tcacctccgc cgcggtcgcg gtcatgggga tgatctctcc
     1741 tgtcgtgagg accggccggt agagctcgac cgcgcgcgcc gtgctgacgt cgtcgatccc
     1801 gccgacgatc cggtcgtgct cgcggatgtt ccggaggagc cgcccgacca tcacccgttc
     1861 gggggcgtga gcgaggcaga agtcctcgcc ggcgacgagc cccgactccg attcgagtat
     1921 ctcacgggcc atcccggcag tcgtccccgg ggtaacggtc gactcgagga ccacgagcgt
     1981 gccctctgag aggtgccttc ccaccgcgcg gagcccctcg ttcagggccg agaaatcagg
     2041 aatcagatct tttgggtcga gaaacggcgt ctggatggcg agagtcaccg catcgcaccc
     2101 tgcgatctcc gagaaatccg acgtgcaccg gaacttcccc gcgttgacga ccttcccgag
     2161 caactcctcg agccccggtt cttcaccctt gagcggcgac tcgccgcggt tgagcatctc
     2221 tatcttgtag cccgaagacg cagaggcgcg ctggaacccg aggaccgact cgaactccgg
     2281 cgcatccgca aagaggaccg cggcggggat accgacgtag cccatcccga cgacaccgat
     2341 ctttctgatc gggcccctgg catcgacgat cgattgtaac gtattgctca taaaaaaacc
     2401 ctcacgccgt aaaacaccgc gcgacgtcct cgcagatctc catattcagc agcgcctgcg
     2461 cggggctgac cgggaactcc cg
//
LOCUS       DAOSII010000238         2411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_37955_length_2411_cov_46.011036, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000238 DAOSII010000000
VERSION     DAOSII010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37955_length_2411_cov_46.011036"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..1803
                     /locus_tag="PLV90_10780"
     CDS             37..1803
                     /locus_tag="PLV90_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOF97641.1"
                     /translation="MSELLTILFPILVGSCAVSALITYSLGAYVLGQNPSSTINRLFF
                     ALALSATCWALGEFFIWQSTSYDGYQFWLRISSFWSFVIALTVHFTLVFTNHPFARED
                     KRWILLVILYLPALLIALIGFSTNLIYTVVFQPGMGYIYQPAAASPAYLASRAYITLA
                     IVWGLYVGISSLRRVRTRRERRQHQLILTGIVTAAGFGAISGLVLPALGVYTVNVVFI
                     GFVLLSLLISYAIHRHGLFALSPQTAVVDILKVMPDGLLLADMDGRVVTANASAAEIF
                     GVAGADLPGQPVRRFIPDTTCDTIRAAIAEQGRFSDLEATPGGREGRTISISGAPVRN
                     PDGESAGVVLIIRDITRRRAAETALRTANQKLSLLSQVTGHDIGNDITGLAWYLNLLS
                     EDRTHPDAGTYLTRSVDIVEDIAKHLAFSRECQMLGAYQSVWQPLGPMIEGAVGSIHH
                     PGVEIAVRVAPVEVYADPLSPKVVYNLLENALRHGGGITRICITTAEGADGMIVVAFE
                     DDGVGVPDDEKERIFCYGYGKNTGFGLAFSRDVLSVTDIAIRETGTAGRGARFEILVP
                     PRAWRRFEGALADPQDAQPAGQ"
     gene            1946..2335
                     /locus_tag="PLV90_10785"
     CDS             1946..2335
                     /locus_tag="PLV90_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOF97642.1"
                     /translation="MRGTATQAKARTIARGKDVVDSSVKNPRGEDLGSIKDVMIDINE
                     GCIAYAALSFGGIMGLGDKLFAIPWAALQYNAADDYFVLDVPRDQLENAPGFDKNNWP
                     TTAEREWLTRMYSHYGYPPYWEHHSGR"
BASE COUNT          463 a          751 c          712 g          485 t
ORIGIN      
        1 cgggagaatt cggatgatgt tatggtgtga ttggccatgt ctgaactcct cacgatcctc
       61 tttcccattc tggtcggctc ctgcgctgtt tcggcgctca tcacctatag cctcggggca
      121 tacgttcttg gacagaaccc ttcatcgacc atcaaccgcc tcttctttgc attagccctc
      181 tccgcgacct gctgggctct cggggagttc ttcatctggc agtcgaccag ctacgacggc
      241 taccagttct ggctgaggat cagctcgttc tggagtttcg tcattgcgtt aaccgtccac
      301 ttcacgctcg tcttcaccaa ccaccccttc gcacgggagg ataagcgctg gatcctcctt
      361 gtaattctct atctccctgc gctcctcatt gccctgattg gtttctccac caacctgatc
      421 tatacggtgg tattccagcc cggaatgggc tatatctacc agccggcagc cgcaagccct
      481 gcctacctgg cctcgagagc ctatattacc ctggcgatag tctggggatt atacgtcggc
      541 atctcgtctt tgcggagggt gcgcacgagg agagagaggc gccagcacca gctcatactc
      601 accggcattg taaccgctgc cggttttggc gctatctcag ggctggtcct tccagctcta
      661 ggagtttata ccgtcaacgt cgtcttcatc gggttcgtcc tgctctccct cctcatctcc
      721 tacgccattc accggcatgg gctgtttgcc ctgagccccc agactgcggt cgtggatatc
      781 ctcaaggtga tgcccgacgg cctgcttctt gccgatatgg acgggcgggt cgtcacggca
      841 aatgcatcgg ccgcagagat attcggtgta gccggggcgg atcttcccgg acagccggtc
      901 aggaggttca tccccgatac gacgtgcgac acgatccggg ccgccatcgc ggagcagggg
      961 aggttctccg atctcgaggc gacgcctggc ggcagggagg gccgcaccat cagcatctcc
     1021 ggggctcctg tccgaaaccc ggatggggag tctgccggcg tcgtcctgat catcagagac
     1081 atcacccgca ggagggcggc ggagaccgcc ctccggacgg ccaaccagaa gctctccctc
     1141 ctctcccagg tgaccggcca cgacatcggc aacgatatca ccggccttgc gtggtacctg
     1201 aacctcctct ccgaggaccg gacgcatccc gacgccggca cgtacctcac ccgatcggtc
     1261 gatatcgtgg aggatatcgc aaagcacctt gcgttctcgc gcgagtgcca gatgctcggc
     1321 gcctaccagt cggtctggca accgctcggg ccgatgatcg aaggagccgt cggcagcata
     1381 caccaccctg gagtcgagat tgccgtccgg gtagccccgg tggaggtcta cgccgacccg
     1441 ctctcgccga aagtcgtgta caacctgctt gagaatgcgc tccggcacgg aggaggaatc
     1501 acccggatct gcataacgac cgccgaaggg gctgatggga tgatcgtcgt ggcgttcgag
     1561 gacgacgggg tgggggtccc ggacgacgag aaggagagga tcttttgcta tgggtacggg
     1621 aagaacaccg ggttcgggct ggcgttttcg cgggatgtcc tctctgtaac cgatattgcg
     1681 atacgcgaga ccggaacggc gggccgggga gcccggtttg agatcctcgt tcctccccgg
     1741 gcgtggagac ggtttgaggg agctctcgca gatccgcagg atgctcaacc ggccggccaa
     1801 tgaagcgata accgccgaaa aacgccggca cccgatttta tacaggaccc aacatattgt
     1861 cacaacgatt aaatactgca cgacctgtag tgacttcctt gccgtggaac agagcgtgtt
     1921 ccacaaacca ggagatgaga cacctatgag aggaactgcc acgcaagcga aggcgcgaac
     1981 tattgcacgg gggaaggacg ttgtggatag ctcggtcaaa aatccccggg gcgaagatct
     2041 cgggagcatc aaagacgtca tgatcgatat caacgagggc tgcatcgctt acgccgccct
     2101 ctcgttcggc ggcatcatgg gcctcggcga taagctcttc gccatcccct gggcggcact
     2161 ccagtacaat gcggccgatg actactttgt cctcgatgtg cccagagatc agctggagaa
     2221 cgcaccgggt tttgataaga acaactggcc gacgacggcc gagcgcgagt ggctcacccg
     2281 catgtacagc cactacgggt atccacccta ctgggagcac cactccgggc gctgagtctg
     2341 ccgggaccaa ccggtcccgg gtcaggtgcg gggccggcat gttgacagag cgtgaccccc
     2401 ggcgacgacc g
//
LOCUS       DAOSII010000239         2357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_39079_length_2357_cov_48.870113, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000239 DAOSII010000000
VERSION     DAOSII010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39079_length_2357_cov_48.870113"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            479..1858
                     /locus_tag="PLV90_10790"
     CDS             479..1858
                     /locus_tag="PLV90_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF97643.1"
                     /translation="MRFYGRERELQLMEHLYARAPSFLVVTGRRRIGKTELIKEFCRG
                     KPALYLYVDANKSITALMEEFGRLAAEALNLPGYIRTDTPETFLEFLFSYDQPLIVVF
                     DEFQRFLKIHPSFISQMQRFWDLAGRDSHLFIIISGSSVGMIREIFLLGDAPLFRRAD
                     NILTLRPFSPRECFVILGDLGVQDPVEQLNLYLLFGGTIYYYTFLEKYGCTNLESALD
                     QLILSDLAPLGREMSDVVVEEFGREYATYYEILGAIALGKRTLKEIADLVSLPSTSLP
                     PYMRDLVDLLGIIEYRVPVTERGKRSKMGRYIFVDNFLRFYARFIYRNMSLYESGRFV
                     LLKGRILGEWKGFSGWAFEEMARTLLARNLAEGYEEIGTWWNRRGDEIDLLAFGPEGS
                     LAIEIKNRDLTLGEARAILAALGEKIPLIKGLAGPVTTGIAARTIEGREELATEGFYT
                     ADLGDIRRI"
     gene            complement(1938..>2357)
                     /locus_tag="PLV90_10795"
     CDS             complement(1938..>2357)
                     /locus_tag="PLV90_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase-like protein"
                     /protein_id="HOF97644.1"
                     /translation="LNDMAMMMLKRFLYLAEGEGLLTGEIPDTVVGIAPSGVPLATLI
                     AAEEGLKLAVYLPAKHSRSEAPTGSLAGTFATITAQRCIIVDDVVTTGTTLAEVIAFL
                     RRHGATPMAVWSLFDKLGAREVDGVPVLSLFTISRLG"
BASE COUNT          442 a          698 c          721 g          496 t
ORIGIN      
        1 ttgcagccat gatattcgag cgctgcgcct cgaatccgcg cgtgcgctca acattttccc
       61 gtaaaataat gatgggctgt ccagcaagca tcgtataatc tcctttgtcc gttaggtttg
      121 ttcagacgtt ctatatatag tgatcgatag gtcgggtcac cccggggtgc ccgggccgtc
      181 gggaggtgcc ggatcgccgg gagatcggtt ctgcacgccc ggggtccggg gggatgcgtg
      241 aggtgcggag tcttcccccg tatccggaag atccgggcag ggcgagggcg gtcggggttg
      301 cccggggcaa acactccggt ttatctctgc agggagaccc ggtccttccg aaaaggggtt
      361 gcaagagtct ctctatcggt gatgggaaaa tgccgctccc aaagagtacc tctccgccgg
      421 cggcggggtg ggtggcaccg taattattat atttgtaatt attatatcca taataactat
      481 gaggttctat ggcagggagc gggaactcca gctgatggag cacctctatg cccgtgcccc
      541 ctcctttctg gtcgtcaccg gcaggcgaag gatcggcaag accgagctga tcaaagagtt
      601 ctgtagaggg aagccggccc tctacctcta cgtcgatgcg aacaagagca tcacggctct
      661 catggaggag ttcgggaggt tggcagcgga agcgctcaac ctccctggtt acatcaggac
      721 cgatacgccg gagacgttcc ttgagttcct cttctcctac gaccaaccgc tgatcgtcgt
      781 cttcgatgag ttccagcgtt tccttaagat ccacccctcg tttatatctc agatgcaacg
      841 gttctgggat ctcgcgggcc gggattcgca tctcttcatc atcatatccg ggtcgtcggt
      901 gggaatgatc cgggagatct tccttctggg ggatgccccc ctcttcaggc gggctgacaa
      961 catcctgaca ctgcgcccct tcagcccaag agaatgcttt gtcatcctcg gcgatctggg
     1021 tgtgcaggat ccggtggagc agctcaacct ctatctcctc ttcggcggga cgatctacta
     1081 ctataccttt cttgagaagt acggatgtac caaccttgag agtgccctcg atcagctcat
     1141 cctgagtgac cttgctcccc tcggccggga gatgagtgac gtcgtggtcg aggagttcgg
     1201 gcgggaatac gcgacctatt acgagatcct cggggccatt gctcttggaa aaagaaccct
     1261 gaaggagatc gccgatctcg tctccctccc ctcgacatcg ctccccccgt acatgcggga
     1321 tctcgttgac ctcctcggga tcatcgagta ccgggttccg gtcaccgaga gggggaaacg
     1381 ctcgaagatg ggcagataca tctttgttga taattttctc cgattctacg cccgctttat
     1441 ctaccggaac atgagccttt acgaaagcgg gcgctttgtc cttctgaagg gccggatcct
     1501 cggcgagtgg aaggggttct ccggctgggc cttcgaggag atggcgcgga ccctcctcgc
     1561 ccggaacctc gcagagggct acgaggagat tggcacctgg tggaaccgcc gtggcgatga
     1621 gatcgatctt ctcgccttcg gcccggaggg gagccttgcc atcgagatca agaaccgcga
     1681 tctcaccctc ggcgaggcac gcgccatcct tgccgctctg ggggagaaga tccctctcat
     1741 aaaaggtctc gccgggccgg tgaccaccgg catcgccgcc cgcaccatcg agggcaggga
     1801 ggagctcgcg acggaagggt tctacacggc ggatctcggt gacatcagga ggatctgagg
     1861 ggccgggcgc gggaggcggg gtggtatccc cgggttctcc tgcccgcgcc tcccgaaaaa
     1921 acaggtagtt ttgactctca cccgagtctc gatatcgtaa agagcgacag gaccgggacg
     1981 ccgtcgacct cccgggcgcc cagtttgtca aagagcgacc agaccgccat cggcgtcgcc
     2041 ccatgccgcc ggaggaacgc tataacctcc gcaagcgtcg tcccggtcgt gacgacgtcg
     2101 tcgacgatga tgcagcgctg ggcggtgatc gtggcgaacg tgcccgcgag ggacccggtg
     2161 ggcgcctcgc tccggctgtg ctttgcggga aggtagaccg cgagtttgag cccttcctct
     2221 gcggcgatga gggtcgcgag cgggacgccc gagggcgcta taccgacgac cgtatccggg
     2281 atctccccgg tcagcagccc ttccccctcc gccaggtaga ggaagcgctt gagcatcatc
     2341 atcgccatat cgtttaa
//
LOCUS       DAOSII010000240         2344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_39363_length_2344_cov_49.192661, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000240 DAOSII010000000
VERSION     DAOSII010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39363_length_2344_cov_49.192661"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1937)
                     /locus_tag="PLV90_10800"
     CDS             complement(<1..1937)
                     /locus_tag="PLV90_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HOF97645.1"
                     /translation="MNRLLIVSNRLPISVTRKNGDFRLQRSVGGLATGVGSFYKAYES
                     LWVGWPGMNVQRKQEEEKDRIVDTLKREQCHPVFLSPYDIKHYYDGFCNNTLWPLFHY
                     FNLFADYDQKTWTVYQRVNERFCDAVMEVVQPDDVIWVHDYHLMLLPEMLRERLPDAE
                     IGYFHHIPFPSFEVFRGLPWRREILAGLLGADLIGFHTYGYVRHFLSSVRRILGYEHS
                     FGEVRTGTRVVRTDLFPMGIDYHRFADSAGSTPVQREIERIRQKYGKRKIILSFDRLD
                     YTKGIPLRLEAFDTLLEKKPEYQGKVSLVLVAVPSRAGVSRYQALKKRIDELVGRING
                     KYGTTDWVPVTYFYNFLPFETLVAFYSAADVALVTPLRDGMNLMAKEYVATRTDGTGV
                     LILSEMAGAAEELGEAIIVNPNDRDAVIEAIEAALAMPEKEQVERNRTMQRRLMRYDI
                     ERWVGDFLTRLGDARAIRVERSEQIVTPAIREQLVDDYAAAQDRLLLLDYDGTLVPFA
                     AKPQKAVPGETTREVLDVLSRVPGNEVVVISGRDRYTLDAWFRGMDIGLIAEHGVWTK
                     ERSGEWRMHEGLSSDWKEEIYPLLELYTDRTPGAFIEEKDFSLVWHYRRTEPILGAQR
                     AKDLKDDLLHLTSNLNVGVMEG"
BASE COUNT          410 a          717 c          756 g          461 t
ORIGIN      
        1 ccctccatga cgccgacgtt gaggttcgac gtcaggtgca ggaggtcgtc tttgaggtcc
       61 tttgcccgtt gcgcgccgag gatcggttcg gtcctccggt agtgccagac gagggagaag
      121 tccttctctt ctatgaacgc gccgggcgtg cggtcggtgt agagttcgag gagcgggtag
      181 atctcctctt tccagtcgct ggagagccct tcgtgcatcc gccactcccc ggaacgctcc
      241 tttgtccaga caccgtgctc ggcgatgagc ccgatatcca tcccccggaa ccaggcatcc
      301 agggtgtacc ggtccctgcc gctgatcacc accacctcgt ttcccggcac acgagagaga
      361 acatcgagca cctctctcgt ggtctcgccg ggcaccgcct tctgcggttt tgccgcgaaa
      421 ggcaccaggg tgccgtcgta atcgaggagg aggaggcggt cctgagcggc ggcatagtcg
      481 tcgaccagct gctccctgat ggccggggtg acgatctgct cggagcgttc gacccggatc
      541 gcccgggcgt cgccgaggcg ggtgaggaag tcgccgaccc agcgctcgat gtcgtagcgc
      601 atcagccgtc tctgcatggt ccggttccgt tcgacctgtt ccttctccgg catggcaagc
      661 gccgcctcga tcgcctcgat cactgcgtcc cgatcgttag ggttgacgat gatcgcttca
      721 ccgagttctt cggccgctcc cgccatctcg gagaggatga gcaccccggt accgtcggtt
      781 cgggtagcga cgtactcctt cgccatcagg ttcatgccgt cgcggagcgg tgtcaccagg
      841 gcgacgtcgg cagcgctgta gaacgccacc agcgtctcga acgggaggaa gttgtagaag
      901 taggtgaccg ggacccagtc ggtcgtcccg tacttcccgt tgatccggcc gacgagttcg
      961 tcgatccgct tcttcaacgc ctggtagcgg ctgacgccgg cccttgacgg gacggcgacg
     1021 aggacgagag agaccttccc ctggtactcc ggtttcttct caaggagcgt atcgaatgcc
     1081 tcgagccgga gcgggatccc tttcgtgtag tcgaggcggt caaacgagag gatgatctta
     1141 cgctttccgt acttctgccg gattctctcg atctccctct ggaccggcgt gctgcccgcg
     1201 gagtcggcga accggtggta atcgatgccc atcggaaaga ggtcggtccg caccacccgg
     1261 gtgccggtcc tgacctctcc gaaggagtgc tcgtatccga ggatacggcg gacgctggag
     1321 aggaagtggc ggacgtagcc gtaggtgtga aacccgatga ggtccgcccc gaggaggccg
     1381 gcgaggatct cccgcctcca gggcaggccc cggaatacct caaacgacgg gaaggggatg
     1441 tgatggaagt aaccgatctc cgcatcgggc aggcgctccc ggagcatctc ggggaggagc
     1501 atcaggtggt agtcgtggac ccagatgacg tcgtcgggct gcacgacctc catcacggca
     1561 tcgcagaacc tctcgttcac cctctggtag acggtccacg tcttctggtc gtagtcggca
     1621 aagaggttga agtagtgaaa gagcggccag agggtgttgt tgcagaaccc gtcgtagtag
     1681 tgcttgatgt cgtacgggga gaggaagacc gggtgacact gctctctctt gagcgtatcg
     1741 acgatccggt ctttctcttc ttcttgcttt ctctgaacgt tcataccagg ccagccgacc
     1801 cagaggctct cgtaggcctt gtaaaaagag ccgaccccgg tggcaagacc accgacgctc
     1861 cgttgcaggc ggaagtcgcc gttcttccgg gtgacgctga tcggaagcct gtttgagact
     1921 atcagtaacc tgttcatggt tgaccctgct ctcgatcgag atgatgatcg caccacaagg
     1981 attctcaacc aaaattaagg ttttggcgaa atctcaaaaa actgtggata tcaggccccc
     2041 ggcgcgcacc ttctctcccc ggctctcccg ccggccgccg acaccgggga tatcgcgcat
     2101 acccgatcgc ggtgattctg ccgggggctt cggggcagag gctgccggag ggagggcggg
     2161 ttatcatgca ccgcccgctg ccgggatgcc ggcctttatc ccgcgcgtga acgaccggcc
     2221 gggaggtttg ccgcgaggga ggccggtgca gggttcggag ggctgaaaca ggcatgtcgg
     2281 agaggctccg ggaaggcagc gcccggaccc gggcgtaccg cacgcagggt atcggcatct
     2341 ccat
//
LOCUS       DAOSII010000241         2338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_39500_length_2338_cov_55.041612, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000241 DAOSII010000000
VERSION     DAOSII010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39500_length_2338_cov_55.041612"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PLV90_10805"
     CDS             complement(<1..497)
                     /locus_tag="PLV90_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA surveillance protein Pelota"
                     /protein_id="HOF97646.1"
                     /translation="MKAEVRELKRRFGEIRLFPETLDDLWHLSHLVGPGDLVFATTFR
                     SVEAATDKLRPEKTEKRPVRLGIRVEKVEFHPHSNRLRIAGVIESGVDVASHHTLNVE
                     TGFELSVIKFWRSVDCERLRRAVDASAYGVVHVVSVEEGEAQVYRLRQFGPEWVTTVT
                     AGSSKG"
     gene            complement(494..>2338)
                     /gene="rqcH"
                     /locus_tag="PLV90_10810"
     CDS             complement(494..>2338)
                     /gene="rqcH"
                     /locus_tag="PLV90_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue protein RqcH"
                     /protein_id="HOF97647.1"
                     /translation="AAVRLPLWVGKIYQFDAKTLGIRLNGEDRAKYQLLVETGRRAHF
                     TAEFPVPPKNPPSFAMLLRKHLEGGKVLGIRQFGIERTVCIDVGKRDTTYRLIFELFD
                     EGNAVLCDEGYTIVKPLWHHRFKTREVVPGAVYTFDGTDCSTLTPDAFGAMLAGSDRD
                     IVRTLAVGCMLGGAYAEEVCRMAGVDKSAAAADVDATAIRASFDRLMADVAGSREPVI
                     TGSGCWPVVLGGEVVRERFATFSEALDAFYPKTAGEKEKAAAEKPRLSQDEVIRRRQA
                     EAIKGFEKKIETNGRVVEVLYENYAPVAGIIATLDAASKNRSWQEIEQILKGNRENAA
                     AKMIRAVHPAEAAVEVDLAGERVKVYVHETIEQNIGRYYDQIKKFKKKKAGALAAMER
                     TVPEKPRRKQNLALQKKRWYHRFRWFTTTDGVLVIGGRDASQNEDLVKKYMEGKDLFV
                     HADVHGGSVVIVKGTTVHLDEVAQFAASYSNAWKAGHFTADVYAARPDQVSKTPESGE
                     YVARGAFIVRGERQYFRNVPLGVAIGLAMEPEVAVIGGPPGAVAARTGVRVELRPGQF
                     EPNDTARKVVRALREKLPEDEWKGLKNALNTEAVAAFVPPGGSDIAEP"
BASE COUNT          343 a          814 c          743 g          438 t
ORIGIN      
        1 cccttgctgc tcccggccgt gacggtcgtc acccactccg ggccgaactg gcgcaggcgg
       61 tagacctgcg cctcgccctc ctcgacgctc acgacgtgga cgacgccgta cgcggaggca
      121 tcgacggccc gccggagccg ctcgcagtcg accgaccgcc agaatttgat gaccgagagc
      181 tcgaaccccg tctcgacgtt cagggtatgg tgcgaggcga cgtccacccc gctctcgatg
      241 accccggcga tgcggaggcg gtttgagtgc gggtggaact ccaccttctc gacccggatg
      301 ccgaggcgga ccggccgttt ctccgtcttc tccggccgga gcttatcggt cgccgcctcc
      361 acgctccgga acgtcgtcgc aaagacgagg tccccggggc cgaccaggtg ggagaggtgc
      421 cagaggtcgt cgagcgtctc cgggaagagg cgtatctccc cgaaccgcct cttcagttcc
      481 cgcacttcgg ccttcatggt tctgcgatat ccgatccgcc gggcgggacg aacgcggcga
      541 ccgcctcggt gttcagggcg ttcttgagcc ctttccactc gtcctccggg agtttctccc
      601 tgagcgcccg gacgaccttc cttgcagtat cgttcggctc gaactgcccg ggccggagtt
      661 ccacccggac gccggtgcgg gcagcgactg cgccgggcgg gccgccgatg acggcgacct
      721 cgggctccat cgcaagaccg atcgccaccc cgagggggac gttcctgaag tactgccgct
      781 cgccgcggac gatgaacgcg ccccttgcga cgtactcgcc ggactcgggc gtcttgctca
      841 cctggtcggg gcgggcggcg tagacgtccg ccgtgaaatg cccggccttc caggcgttcg
      901 agtaggacgc ggcgaactgc gcgacctcgt ccaggtgaac ggtcgtaccc ttcacgatga
      961 cgacgctccc gccgtggacg tcggcgtgga cgaagaggtc tttgccctcc atatacttct
     1021 tgacgagatc ctcgttctgg gaggcatccc ggccgccgat gacgaggacg ccgtcggtgg
     1081 tggtgaacca gcggaaccgg tgataccacc gcttcttctg gagggcgagg ttctgcttcc
     1141 gccggggttt ctccgggacc gtccgctcca tggccgcgag agcgcccgcc ttcttcttct
     1201 tgaacttctt gatctggtcg tagtagcggc cgatgttctg ctcgatcgtc tcgtggacgt
     1261 agactttgac ccgctccccg gcaaggtcga cctccaccgc cgcctcggcc gggtggaccg
     1321 cccggatcat ctttgccgcc gcattctcgc ggtttccttt gaggatctgt tcgatctcct
     1381 gccacgagcg gtttttgctc gccgcatcga gcgtcgctat gatcccggcg acgggagcgt
     1441 agttctcgta gagcacctcc acgacccgcc cgttcgtctc gatcttcttc tcaaacccct
     1501 tgatcgcctc cgcctggcgc cggcggatca cctcgtcctg ggagaggcgg ggtttctcgg
     1561 cggcggcctt ctccttctcg cccgccgtct tcgggtagaa cgcatccagg gcctcgctga
     1621 acgtcgcgaa ccgctcgcgg accacctcgc cgccaagcac caccggccag caccctgacc
     1681 ctgttataac gggttcccgg ctccccgcga cgtcggccat caggcggtcg aaggacgccc
     1741 ggatcgccgt ggcatcgaca tctgcggcgg cggcgctctt atcgacgccc gccatccggc
     1801 agacctcttc ggcatacgcg ccgccgagca tgcacccgac ggcgagcgtc cggacgatgt
     1861 cgcggtcgga cccggcgagc atcgccccga acgcatcagg tgtgagggtg gagcagtcgg
     1921 tcccgtcgaa ggtgtagacc gcgcccggga ccacctcccg ggtcttgaac cggtggtgcc
     1981 agaggggttt gacgatcgtg tagccttcgt cgcagaggac cgcgttcccc tcgtcgaaga
     2041 gctcgaatat gaggcggtag gtcgtatccc tcttcccgac atcgatgcag acggtgcgct
     2101 cgataccgaa ctggcggata ccgagcacct tcccgccctc gaggtgcttt cggagcagca
     2161 tcgcaaagct cggcggattt tttgggggaa cggggaactc cgcggtgaaa tgggcgcgcc
     2221 tcccggtctc gacgagcagc tggtactttg cccggtcctc cccgttcagc ctgataccaa
     2281 gggtctttgc atcgaactgg tagatcttgc cgacccagag cgggagacgg acggcggc
//
LOCUS       DAOSII010000242         2336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_39546_length_2336_cov_49.770715, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000242 DAOSII010000000
VERSION     DAOSII010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39546_length_2336_cov_49.770715"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PLV90_10815"
     CDS             <1..467
                     /locus_tag="PLV90_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97648.1"
                     /translation="LGERYTYDDPSGENMVSLLVESTRTVATYEAVPTNGSTTQVEAG
                     DGRTFLLVVVKGTNLGHKGDSDLYTIETPGRDAFTLEYQGTVFTPLDVPAYTLYGESF
                     DKKTLERYESEKGYLYFDVPVTFEASGAILRADLGDAGTPAWDLGREPGSAA"
     gene            complement(475..984)
                     /locus_tag="PLV90_10820"
     CDS             complement(475..984)
                     /locus_tag="PLV90_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOF97649.1"
                     /translation="MRIGILSDTHDNLDMTDAAVRRLNREGVDLTLHAGDYVSPFVIP
                     RLANLQAPMVGVLGNNDGDHGLLAARFAEHGHLSLRGAFAAVDAGDVAIGLLHGDDRE
                     LLQALIGRKAFDIVVHGHTHKADVRDLGDILVVNPGEACGYLTGRPTIAVLDTATRHA
                     EVLSLRSER"
     gene            1235..1405
                     /locus_tag="PLV90_10825"
     CDS             1235..1405
                     /locus_tag="PLV90_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97650.1"
                     /translation="MWAYIKRSDGRTEEREFPAGVYIEVGDILEDGSVVIDLPYSDDI
                     EDDADPLTLYDD"
     gene            complement(1415..>2336)
                     /locus_tag="PLV90_10830"
     CDS             complement(1415..>2336)
                     /locus_tag="PLV90_10830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF97651.1"
                     /translation="AVLRDARKHVLIVPQPGRNASPLFGRLLAPTDLAVLAPDVRSVL
                     EEPAEGGTAVLLHVVEPGRPGTEQEAGDRLAHLAGGLSPAGRTIRTMVRTGIPARTIC
                     AVADEIGASLVVIPRVGKRDAPGNAPLGSVTSAVVGCANPPVLVLAVPVRLKVEGREL
                     RRDEFALAEELWIDYHQLKGDPGVDRIFGVFADDTLVSIARCRRHPDGYEVDGVFTPV
                     RFRGKGYARLAMDALVEACQHDTLYMHSVRNLVDFYAAYGFVSIPESGLPPTIQARFA
                     FALGEMEGANVQPMRRVQGFFRPARRSAGR"
BASE COUNT          408 a          789 c          750 g          389 t
ORIGIN      
        1 cgctcgggga gcgctacacc tacgacgacc cctcaggcga gaacatggtc tcgctgctcg
       61 tggagtccac ccggaccgta gcgacctacg aggcggttcc caccaacgga tcgaccacgc
      121 aggtcgaggc aggcgacggg cgcaccttcc tcctcgtcgt cgtcaagggg acaaacctcg
      181 ggcacaaggg cgactccgat ctctatacca tagagacgcc cgggcgggat gcgttcaccc
      241 tcgagtacca ggggacggtc ttcacgccgc tcgatgtccc ggcctatacc ctgtacggcg
      301 aatcgttcga caagaagacg cttgagcggt acgagtcgga gaaaggatac ctctacttcg
      361 acgtgcccgt aaccttcgag gcatccgggg cgatcctccg cgccgatctc ggggatgccg
      421 gcacccctgc ctgggatctc gggagagaac cagggagcgc ggcataaccc gcacctatct
      481 ttcggatcgg agggagagca cctccgcatg ccgggtcgcc gtatccagga cggcgatggt
      541 cggccggccc gtcaggtatc cgcaggcctc cccggggttg acgaccagta tatccccgag
      601 gtccctgaca tccgccttat gggtgtggcc gtggacgacg atatcgaagg ccttccgtcc
      661 tatgagcgcc tgcaggagct cgcggtcgtc cccgtggagc aggccgatcg ccacgtcgcc
      721 tgcgtcgacg gcggcaaacg ccccccgcag gctgaggtgg ccgtgttcgg caaaccgtgc
      781 cgcgaggagg ccgtggtcgc cgtcgttgtt cccgagaacg ccgaccatgg gtgcctgcag
      841 gttcgcaagc cggggtatga cgaacggcga tacgtaatcg ccggcatgga gggtcaggtc
      901 caccccttcc cggttcaggc gtctcacggc cgcgtccgtc atatcgaggt tgtcgtgagt
      961 gtcagagagt atcccgatgc gcatatacgt tatcctgacc gctcccacga tatatatggc
     1021 tgtgcatcat accggccggg gcggatcgcc ttcctatccg cgcttttccc tagcttgatc
     1081 tccggatccg cgatcgtcgc tacgttctcc ggttcgctcc accaaaacat ctttattcgg
     1141 ctatcatctg atactggaat agaaacacgc tcccgggggg cgccgggctc cggcccggca
     1201 ccccggggga acggtagatt cacggaggca gagcatgtgg gcatacatca aacgttctga
     1261 cggcagaaca gaagagcggg agttccctgc aggggtctac atcgaggtcg gcgatatact
     1321 cgaagacggc tcggtcgtca tcgatctccc gtactccgac gatatcgagg acgatgccga
     1381 ccccctgaca ctctacgacg actgaacagg gccgtcaccg gccggcggat cgccgcgccg
     1441 gccggaagaa accctgcacc cggcgcatcg gctggacgtt tgccccctcc atctcgccga
     1501 gggcaaacgc gaacctcgcc tggatggtcg gcggaaggcc gctctcgggt atcgagacaa
     1561 acccgtacgc cgcatagaag tcgaccaggt ttcggaccga gtgcatgtag agggtgtcgt
     1621 gctggcacgc ctcgacgagc gcatccatcg ccaaccgggc ataccccttt cctctgaacc
     1681 tgaccggggt gaagaccccg tccacctcgt agccgtccgg gtgccgccgg caccgggcga
     1741 tggagacgag ggtatcgtct gcaaagacac cgaatatccg gtccactccc gggtctccct
     1801 tcagctggtg gtagtcgatc cagagctctt cggcgagcgc gaactcgtcc cgccggagct
     1861 cccgcccctc taccttgagg cgaacgggga ctgccagcac cagtaccggc gggttcgcgc
     1921 acccgacgac ggcgctcgtg acgctcccga ggggggcgtt ccccggggcg tcgcgctttc
     1981 caacccgcgg gatgacgacc agggaggcgc cgatctcgtc tgccacggca catatggtgc
     2041 gcgccgggat accggtccgg accattgtgc ggatggtgcg cccggcgggc gagaggcccc
     2101 ccgcgaggtg ggcgagccgg tcgcccgcct cctgctccgt ccccgggcgc ccgggctcga
     2161 cgacgtgcag gagcaccgcc gtcccgccct ctgcgggctc ttccaggacc gaacggacat
     2221 ccggcgccag gaccgcgaga tcggtcggcg ccagaagtcg tccgaagagc ggtgaggcgt
     2281 ttcttcccgg ctgcggcacg atcaggacgt gcttccgggc atcccggagg acggcg
//
LOCUS       DAOSII010000243         2323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_39817_length_2323_cov_53.225750, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000243 DAOSII010000000
VERSION     DAOSII010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39817_length_2323_cov_53.225750"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1884)
                     /gene="infB"
                     /locus_tag="PLV90_10835"
     CDS             complement(103..1884)
                     /gene="infB"
                     /locus_tag="PLV90_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOF97652.1"
                     /translation="MAKQSTIRTPIVCVMGHVDHGKTSLLDRIRGSSVVSTEEGAITQ
                     HIGATLVPIDAITRMSGALSKVSVNVPGLLFIDTPGHHAFTTLRARGGALADMAIVVV
                     DINEGFRPQTIEALQILRNYKTPFVIAANKIDRIHGWRAQAGQPFMKTFSQQNERVQG
                     ILETKVYELVGKLSDLGFNSERFDRVSDFARNICIVPTSALTGEGIPDILMVLIGLAQ
                     RYMTENLKVSTDGPGAGTVLEVKEERGLGMTLDLILYDGTLKVGDEIVVAGNDQIIET
                     KVRSLLKPRPMSEILLEERFERVKSVTAAAGIKVAAPRLDGVIAGSPLRAIRGGNRDE
                     AIERVRHEVQDIQVNLSDVGVIIRADTIGALEALSKELEGHQIQVMRAAVGPVTRHDV
                     IEAGTIKDPLYSAIIAFNTPVLPDAVDTLADTSMSHVSIFEGGVIYQLLDDYVEWREE
                     KKQELERQQFEKLIMPAKIRILPNCVFRQSNPAVVGVRVLGGKLQSGVDLALPSGKRV
                     GRIKQIQAKNETVQEAEAGKEVAISIDGPTVGRQINVDDDLYVDVPERHVKVIEREMI
                     NQLSASLRETLEEFTTLRRREDPFWGK"
     gene            2249..2321
                     /locus_tag="PLV90_10840"
     tRNA            2249..2321
                     /locus_tag="PLV90_10840"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2283..2285,aa:His,seq:gtg)
BASE COUNT          394 a          715 c          731 g          483 t
ORIGIN      
        1 aaagtatcta tacaatttga taatatggta ttaaaagcct acctccgcgg ggttgaaagg
       61 gaacggtggt cggggatacc cgccggagcg cgcaaagagg gttcacttcc cccagaaggg
      121 gtcttcgcgg cgccggaggg tggtgaactc ctcgagggtc tcgcgcaggc tcgcgcttaa
      181 ctggttgatc atctcccgct cgatcacctt cacgtgccgc tccggtacgt ccacgtagag
      241 gtcgtcgtcg acgttgatct gccggccgac tgtcggtcca tcgatggata tcgccacctc
      301 ttttcccgcc tccgcctcct ggaccgtctc gttcttcgcc tgtatctgct tgatgcggcc
      361 caccctcttt ccgctcggga gggcaaggtc gacaccgctc tggagtttcc ccccgaggac
      421 acggacgccg accaccgccg ggttgctctg ccggaagacg cagtttggga ggatccggat
      481 cttggccggc atgatcagtt tctcaaactg ctgccgttcg agctcctgct tcttttcctc
      541 gcgccactcg acgtaatcgt cgaggagctg gtagatgaca ccgccctcga atatgctgac
      601 atgggacatc gaggtgtccg ccagggtgtc gaccgcgtcc ggcaggaccg gcgtgttgaa
      661 ggcgatgatc gcgctgtaga gggggtcttt gatcgtcccc gcctcgatga cgtcgtggcg
      721 ggtgaccggc ccgacggcgg cccgcattac ctggatctgg tgcccctcga gctccttcga
      781 gagggcttcg agggcgccga tggtgtccgc ccggatgatg accccgacat cggagaggtt
      841 cacctggatg tcctgaacct cgtgacggac ccgctcgatc gcctcgtccc ggttccctcc
      901 ccggatggcc cggagggggg agcccgcgat gaccccgtca agcctcgggg cggcgacttt
      961 gatccccgct gcggcggtga cggacttgac ccgctcgaac cgctcctcga gcaggatctc
     1021 gctcatgggg cggggtttta agagggagcg cactttggtc tcgatgatct ggtcgtttcc
     1081 cgcgacgacg atctcatccc cgaccttgag ggtcccgtcg tagaggatca gatcgagggt
     1141 cataccgagc ccccgctcct ccttcacctc gaggacagtg ccggcgccgg ggccgtcggt
     1201 gctgaccttg aggttctcgg tcatgtagcg ctgggcaagc ccgatcaaca ccatgaggat
     1261 atccggtatg ccttcgccgg tgagggcgct cgtcggcacg atgcatatgt tccgcgcgaa
     1321 gtcggagacc cggtcgaacc gttcggagtt gaacccgagg tcggagagtt tgccgacaag
     1381 ctcgtagacc ttcgtctcga gtatcccctg gacacgttcg ttctgctgcg agaacgtctt
     1441 catgaacggt tggcctgcct gggcgcgcca gccgtggatg cggtcgatct tgtttgccgc
     1501 aatcacgaac ggtgttttgt aattgcgcag gatctgaagc gcctcgatcg tctgcgggcg
     1561 aaacccctcg ttgatatcca ccacgacgat cgccatatcg gcgagcgccc ccccgcgcgc
     1621 acggagggtg gtgaaggcat ggtgcccggg ggtgtcgatg aagaggaggc cggggacgtt
     1681 gacgctgacc ttgctcaacg cgccgctcat ccgggtgatc gcgtcgatgg ggacgagtgt
     1741 ggcgccgatg tgctgcgtga tcgcaccctc ctcggtcgag accacggatg aaccccggat
     1801 cctgtcgaga agcgacgtct tgccgtggtc cacgtgaccc atcacgcaga cgatgggggt
     1861 tctgatcgtt gattgctttg ccatggttat caccttccgg tatatacgag ttatggacgg
     1921 ataatcccca aaaaacctgg aatattgacc gtttttcctg aatcatgccg tggtccggat
     1981 gatcccccta cccccggggt gccctccggc acccggaata ctggtacgtt atagagcatg
     2041 gggtctatta atgattctcc cgggcaccca ctccgctccg gccgggatca ccagacccgg
     2101 caaccgtgcg ccaggatacg cggcaggtcc gggtcgccgg cgatcccggc ccggcatgca
     2161 cccgcgatcg ccgggggcgg gagagggtgc cgaggcgatg aagagatacc ttaatcaaat
     2221 ccgaccgcaa actggtatga gtacaattgc cagggtgggg tagtcggtta tcctaaggga
     2281 ctgtggatcc ctcgacccgg gttcgaatct cggccctggc ctt
//
LOCUS       DAOSII010000244         2304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_40238_length_2304_cov_52.463317, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000244 DAOSII010000000
VERSION     DAOSII010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40238_length_2304_cov_52.463317"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..898
                     /gene="thiL"
                     /locus_tag="PLV90_10845"
     CDS             32..898
                     /gene="thiL"
                     /locus_tag="PLV90_10845"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOF97653.1"
                     /translation="MDERGLHRLIGTIIDPERLSDDCAVIPCGDLLMVASTDMLHETT
                     DFPAGMTDWQVGWMAVAVTLSDIASMGAVPGQVLLAVGLDRPERLAGIMTGARDCCTA
                     FGAELVGGDLDAHRELTIVSTGLGTVRPEHIARRRGARPGDVITVTGPLGEAEAALAG
                     YEQHRKELLEPQPRVAEGESLGRAGVSAMMDISDGLALSLYDLLAVNDCGFAIETARL
                     PLPAGVPEQEGRELALHGGGDFELLFTAPADILPVPGVRTHVIGRVIPERTVLADGKP
                     LERRGYLHEWGG"
     gene            complement(895..1680)
                     /locus_tag="PLV90_10850"
     CDS             complement(895..1680)
                     /locus_tag="PLV90_10850"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HOF97654.1"
                     /translation="MDPLVVATFAVVILLEIIVPLALGYWIVRRFGVPWRIFLLGALF
                     FIAVQVVHTPLVLVTQSPLYLALLPSGTTMALAGLAVYLGLMAGLFEEIGRYVVYRYY
                     FRRRGIPLTRETGLQFGAGWGGVESIFVALLVLSSMVSYIALTSDGGMVPLPDDPAVQ
                     AQVETLKALTPLDILPGLAERMMTITLHVAWSLLVLASLVYGERLLLVLAVLWHAAVD
                     AAAVYLAATAGILVTEAVVFVFAVIGLIYILWEWRRMGARTAS"
     gene            1801..>2304
                     /locus_tag="PLV90_10855"
     CDS             1801..>2304
                     /locus_tag="PLV90_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOF97655.1"
                     /translation="MNTERPGAAGGGGSVPGGRFSNNFDFLRFAAAVAIVILHAYALR
                     LGYTGIGLSNPFVLMGQAGLAVLLVTSGYLIAASWESTASPLRFAWKRFLRVVPALIP
                     AVLITLFVIGPLMTTLSPGEYFNALFSPAGLATIPFFEDGSAIGLFQENPWTYVNGSL
                     WTIPVEVA"
BASE COUNT          426 a          697 c          804 g          377 t
ORIGIN      
        1 cggagcgagc gggagaagga ggtgcgtcgc ggtggatgaa cggggcctgc accggctgat
       61 cgggacgatc atcgacccgg agcggctcag cgacgactgt gccgtcatcc cctgcggaga
      121 tctccttatg gtcgcgagca ccgatatgct ccacgagacg accgattttc ccgccgggat
      181 gaccgactgg caggtgggct ggatggcggt ggcggtcacg ctctccgata tagcgagcat
      241 gggggcggtg ccggggcagg tcctcctcgc ggtcggcctt gataggccgg aacggctcgc
      301 cggtatcatg acgggcgccc gggactgctg cacggcgttc ggcgccgagc tcgtgggcgg
      361 ggatctcgat gcccaccggg aactgacgat cgtgagcacg ggccttggga cggtcaggcc
      421 cgagcatatc gctcgaagga ggggggcgcg gccgggagac gtcatcacgg tcaccgggcc
      481 gctcggggag gccgaggcgg cccttgccgg ctacgagcag cacaggaaag aacttctcga
      541 accgcaaccc cgggtggcgg agggtgagag cctcggccgt gccggcgtct cggcgatgat
      601 ggatatatcc gacggcctcg ccctctcgct ctacgacctt ctcgcggtga acgactgcgg
      661 gtttgccatc gagaccgccc gtctccccct ccctgccggc gtcccggaac aggaggggcg
      721 ggaacttgct ctccacggcg gaggggactt tgaacttctc ttcaccgccc ccgccgatat
      781 cctcccggtc ccgggcgttc ggacgcacgt catcggcagg gtcatacccg agcggacggt
      841 cctcgcggac ggaaaaccgc ttgaacgccg gggatacctg cacgagtggg gcggctagga
      901 ggccgttctt gcccccatcc gccgccattc ccacaggata tagatgagac caatcaccgc
      961 aaagacgaat accaccgcct cggtcacgag aattcccgct gtcgcggcaa ggtagacggc
     1021 cgcggcatcg acggcggcgt gccagaggac ggcgaggacg aggagcaatc tttcgccgta
     1081 gacgagcgag gcgaggacga ggagcgacca ggcgacgtgg agggtgatcg tcatcatccg
     1141 ctcggcaagg ccgggcagga tatcgagcgg cgtgagggcc ttgagggtct cgacctgtgc
     1201 ctggaccgcg gggtcgtcgg ggagcgggac cattccgccg tcgctcgtga gggcgatgta
     1261 ggagaccatg ctcgaaagga cgaggagcgc gacaaatatg ctctcgacac cgccccaccc
     1321 ggccccgaac tggaggccgg tctcgcgggt gagggggatg ccccgccgcc ggaagtagta
     1381 acggtagacg acgtagcggc cgatctcttc aaagaggccg gccatgaggc cgagatagac
     1441 ggcaaggccg gcaagggcca ttgtcgtgcc ggacggcaga agggcgaggt agagcgggct
     1501 ctgtgtcacg aggacaagcg gggtgtggac gacctgcacg gcgatgaaga agagggcgcc
     1561 gaggagaaag atccgccagg gaacgccgaa cctccggaca atccagtaac cgagcgcgag
     1621 cgggacgatg atctcaagca ggatgacgac ggcgaacgtg gcgacgacca gcgggtccat
     1681 tgaggaggat gttctggccg catatttaag ggttgttgcc ggcggcacga gaaccaaaga
     1741 atattttgac taaatattta acgattgtcg ctgtacaatg aggaaaggaa tccgactgcc
     1801 atgaatacag aacgcccggg agcggccggc ggagggggga gcgttccggg ggggcggttc
     1861 tcgaacaact tcgacttcct gcggttcgct gcggcggtgg cgatcgtcat cctgcacgcc
     1921 tacgcgctca ggcttggcta cacaggcata gggctttcca atccgttcgt gctcatggga
     1981 caggcgggac ttgccgtcct cctcgtcacg agcggttacc tgatcgccgc gagctgggag
     2041 tcgaccgcat caccgctccg gttcgcctgg aaacggttcc tccgggtcgt cccggcgctt
     2101 attcctgcgg ttctcatcac gctctttgtt atcggtccgc tgatgacgac cctttccccg
     2161 ggggagtact ttaacgctct cttctccccg gcggggcttg cgaccatacc gttcttcgag
     2221 gacggatccg cgataggcct cttccaggag aacccgtgga cctacgtcaa cggctcgctc
     2281 tggacgatac ccgtcgaggt cgcc
//
LOCUS       DAOSII010000245         2301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_40314_length_2301_cov_52.288958, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000245 DAOSII010000000
VERSION     DAOSII010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40314_length_2301_cov_52.288958"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PLV90_10860"
     CDS             <1..672
                     /locus_tag="PLV90_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOF97656.1"
                     /translation="FRPYQTGDDPNLIDWKITARRDTLYVRQMTGLEGGAPLIAVDLP
                     GRAGDPAAFARFSMAVSGAVEGAISARDGCSLLIVAGPEIVRFLPRTTEIREALAALG
                     GLAPLEPRAPLYRAPGPAVLAARARVPRQGRGEEETAYLARLCGALSAFAGENPALFA
                     AAVRTAFSVAEAREVRVYSLLPPGDRSHLIQLAGEAKIRGMRVVLKAPAGTGTLPGVD
                     TVEVI"
     gene            672..959
                     /locus_tag="PLV90_10865"
     CDS             672..959
                     /locus_tag="PLV90_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97657.1"
                     /translation="MEIDRKFLLLCAAEVLVVAVDNLILALILQVIALGAFVGDRRGY
                     PVFVAGAILFAAVVASAGNVALIGLAAALGCGYLILTLYDYRLTRWAGGEA"
     gene            956..1747
                     /locus_tag="PLV90_10870"
     CDS             956..1747
                     /locus_tag="PLV90_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97658.1"
                     /translation="MTTKSPYRGAFILIIAAAALLALAALTGRGDITTATLVLAGTGC
                     FIAGVFLLALHKGEPLDPDIAALLPAQGTIGIATLCADLGVQGNAWFVPEEETVIEVI
                     PVADTLPEKTGDDYSFITREGECAVRLAPACTPLLARLREKHALEVPEENEALLACIT
                     EVCDDLLEIAEKTEAKRDGENIVVTLSGYRLIPGCRTIRAASPKCCTMVACPVCSLIA
                     AIAALGTKMPVTIEHIDVDEKRKSLRMILTPAGPAPRGAGPGNSR"
     gene            complement(1695..>2301)
                     /locus_tag="PLV90_10875"
     CDS             complement(1695..>2301)
                     /locus_tag="PLV90_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1616 domain-containing protein"
                     /protein_id="HOF97659.1"
                     /translation="AWYRRLLLPAGDRFVVPARAMLAAARLEFFDPGASRLDRGLSAL
                     LLVSIVAALATTAYVIAVPKEGEHFTEFYILGPGGKAADYPTDFPAGATRSLIIGVGN
                     HEYREIPYTVEAFALNQTFDTTTNTSTIHAVERLDRFVVTVPHNETEEIPWEFAVPST
                     AYNRIEFLLFNETVPGEDITGQDRINASYRDLHLWVRVRPA"
BASE COUNT          364 a          826 c          745 g          366 t
ORIGIN      
        1 ttccggccct accagacggg cgacgacccc aacctgatcg actggaagat caccgcccgg
       61 cgtgacaccc tctacgtccg gcagatgacc gggcttgagg gcggcgcccc cctcatcgcc
      121 gtcgacctcc cgggccgggc cggagacccg gcagccttcg cccggttctc gatggccgtc
      181 tccggcgccg tggaaggcgc gatcagcgcc cgcgacggct gctctcttct gatcgtcgcc
      241 ggccccgaga tcgtccgatt cctcccgagg acgacggaga tccgtgaagc cctcgccgct
      301 ctcggcgggc ttgctcccct cgagccccgc gcccccctct accgtgcgcc ggggccggcc
      361 gtccttgccg cccgggcaag agtgccccgg cagggtaggg gagaagaaga gaccgcctac
      421 ctggcaaggc tctgcggcgc cctctccgcg tttgcaggcg agaacccggc actcttcgcg
      481 gcagcggtgc ggacggcgtt cagcgtcgca gaggcacgag aggtccgggt ctactccctc
      541 ctcccgccgg gcgacagaag tcacctcatc cagctcgccg gcgaggcgaa gatccggggt
      601 atgcgggtcg tcctgaaggc ccccgccggc accgggaccc ttccgggcgt cgacaccgtg
      661 gaggtgatct gatggagatc gatcgaaagt tcctcctcct ttgcgcggcc gaggtcctgg
      721 tcgtcgcggt ggataacctc attctagccc tcatcctcca ggtgatcgcc ctcggcgcgt
      781 tcgtcggcga ccggcggggc tacccggtct tcgtcgccgg ggcgatcctc tttgccgcgg
      841 tcgtcgcgag cgccgggaac gtcgccctca tcggtcttgc cgccgctctc ggttgcggct
      901 acctcatcct caccctctac gactaccggc tcacgcggtg ggcaggaggc gaggcatgac
      961 cacgaaaagt ccctaccggg gagccttcat cctcatcatc gccgcagcgg cccttctcgc
     1021 ccttgcggcc ctgaccggca ggggggacat caccaccgca accctcgtcc tcgcgggcac
     1081 cgggtgcttc atcgccggtg tcttcctcct cgccctccac aagggcgagc ccctcgaccc
     1141 ggatatagcc gccctcctcc ccgcccaggg gacgatcggg atcgccaccc tctgcgcgga
     1201 cctcggcgtc cagggcaacg cctggttcgt cccggaggaa gagaccgtca tcgaggtcat
     1261 accggtcgcg gataccctcc cggagaagac cggcgatgac tactccttca tcaccagaga
     1321 aggcgagtgc gccgtccggc tcgccccggc atgcaccccg ctcctcgccc gtctccgcga
     1381 gaagcatgcc ctcgaggtgc cggaggagaa cgaagccctc ctcgcctgca tcaccgaggt
     1441 ctgcgacgac ctcctcgaga tcgccgaaaa gactgaggcg aagagagacg gcgagaacat
     1501 cgtcgtcacc ctctcgggct accgcctcat accgggctgc cggaccatcc gggcggcctc
     1561 gccgaagtgc tgcaccatgg tcgcctgccc ggtctgcagc ctcatcgccg ctatagccgc
     1621 gctcgggacg aagatgccgg tgacgatcga gcacatcgac gtcgacgaaa agagaaagag
     1681 cctccggatg atcctcacgc cggccggacc cgcacccaga ggtgcaggtc ccggtaactc
     1741 gcgttgatcc ggtcctggcc cgttatatcc tccccgggaa ccgtctcgtt gaagaggaga
     1801 aactcgatcc ggttgtaggc ggtcgacggc accgcaaact cccaggggat ctcttctgtc
     1861 tcgttatggg ggaccgtcac cacgaaccgg tcgagacgct cgaccgcatg gatggtcgac
     1921 gtgttcgtcg tggtatcgaa cgtctggttg agggcgaacg cctcgaccgt gtaggggatc
     1981 tcccggtact cgtggttccc gacccctatg atgagcgacc gggttgcgcc ggccggaaag
     2041 tcggtcgggt agtccgcggc cttcccgccg gggccgagta tgtagaactc ggtgaagtgc
     2101 tccccttctt ttgggaccgc gatgacgtag gcggtcgtcg cgagcgcggc gacgatcgat
     2161 accaggagga gggcggagag gccccggtcg agccgggagg cacccgggtc gaagaactcc
     2221 aggcgagccg cggcaagcat cgcccgggcc gggacgacga accggtcgcc ggccgggagg
     2281 aggaggcgcc ggtaccaggc g
//
LOCUS       DAOSII010000246         2297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_40420_length_2297_cov_27.535236, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000246 DAOSII010000000
VERSION     DAOSII010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40420_length_2297_cov_27.535236"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            473..868
                     /locus_tag="PLV90_10880"
     CDS             473..868
                     /locus_tag="PLV90_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOF97660.1"
                     /translation="MKEAERWLRQGERDLLGARNSCRSGNFEWACFQAQQSAEKSLKA
                     LLYARGFRRILTHSVYELIREVGQFEPSFLELKAGAKALDSAYIMARYPDSIVGNLTP
                     SEYYDREDAGECIGYADSICNAARQALAG"
     gene            865..1155
                     /locus_tag="PLV90_10885"
     CDS             865..1155
                     /locus_tag="PLV90_10885"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOF97661.1"
                     /translation="MIGEVRNLAAAIRSRRRVSAIILYGSFARGDFHEESDIDLVVVG
                     DFRERPHKRAATILGLTDLPVEPLCYTEEEFAELVRDENPFILRALAEGIRV"
     gene            complement(1598..1888)
                     /locus_tag="PLV90_10890"
     CDS             complement(1598..1888)
                     /locus_tag="PLV90_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HOF97662.1"
                     /translation="MYELIFSEEALKYLSQIPKKDLAHVREVISLCLREFLKKATRRC
                     NKKLIKGSKKHTYRLHISMTHTAFYRIDDENERVLIDDMMGINQAHGKYGLY"
     gene            complement(1881..2111)
                     /locus_tag="PLV90_10895"
     CDS             complement(1881..2111)
                     /locus_tag="PLV90_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97663.1"
                     /translation="MTQSVSPDNSDMVSIRVSRATQEKLKKLKKGNMTYDDVISQMVD
                     DENWDVEKLDADLDIIEKKSKFYTFEEVFKGV"
BASE COUNT          503 a          677 c          674 g          443 t
ORIGIN      
        1 cgaagagagg tcggcgggaa tctcccggcc ggagatcatc gaggagatcg agaacaaagt
       61 ggccccatgc ctctggtaga ccgccttctc ggcaccctgc tgagatatga aacaggccca
      121 ctcaaagaac ccggtatccc ggccatggcg agatcccgcc gcgcctgctc catccggtcc
      181 gcactccgct cggccatacc gcatgagaat agagaggcgc aatatttgaa tgcagggttc
      241 gggcccgccc cgcccggggg atctgccgct tccccgggcg cgacggcatt cctcaaagcg
      301 gaaggagacc tcatgcagag gcgacgggtg ccggcgacgg cacccgcgga ctcaaagcct
      361 cagcgatgac ggccccgggg aggccgacta tcgccccgcg aggagacccg cggcgcactt
      421 atatcctccg ggcaacaacg catatcatcg ggcaggtact atggacgcga atatgaagga
      481 agcggagcgg tggctgcggc agggagagcg ggatcttctc ggcgcgcgga acagctgccg
      541 gtcgggcaac ttcgaatggg catgctttca ggcgcagcag agtgcggaaa aatcgctaaa
      601 agcactgtta tacgcccggg ggttccggcg gatcctgaca cactcggtct acgaactaat
      661 ccgggaagtc ggtcagttcg agccctcgtt cctggagctg aaagccggag caaaggcgct
      721 cgacagcgcc tacattatgg cgcgatatcc ggacagcatc gtgggaaacc tgacgccgtc
      781 agagtactat gacagggagg atgccgggga atgcatcgga tacgcggact cgatctgcaa
      841 cgccgcgagg caagcgctcg ccgggtgatc ggagaggtcc ggaacctcgc ggcggcgatc
      901 cggtcccgcc ggcgcgtctc cgccatcatc ctctacgggt cgtttgcccg cggcgacttt
      961 cacgaggaga gcgacatcga cctcgtcgtc gtcggggact ttcgcgaacg gccccacaaa
     1021 cgggcggcga ccatcctcgg tctgaccgac ctcccggtcg aaccgctctg ttacaccgaa
     1081 gaagagtttg ccgaactggt ccgggacgaa aaccccttca tcctccgggc cctggccgaa
     1141 ggcatccggg tgtaaaaacc cccgcgcgca gagcggggcc ggcgagaagc ctctatccac
     1201 ggggggtgtt tacggatcga gcagcgcacc cccaccgggc agcttcaacc gaaaagagcg
     1261 tcggatctga agccgtgggc attccgtctc gtaaaggccc tgctcccgga tggttgcaag
     1321 cgggacgggg tatcaggttg acgatgattc tggatggcac cccgtatgcc gccggcaaca
     1381 ccatccggcg agcagatctt ccgtcatatc caccggatca aagtcccatc gagcaggtcc
     1441 ggcgggcgac caggcaggag ggctccggcg acgttcgcca cgggctacga agatgtgact
     1501 gccacgattg tacgggcaag aacccggtgg ctgaaaaata cgggcaaagg catcccgagc
     1561 ccaacgtatg gctgcaggta gccagtaaaa aaaagggtca gtacaaccca tatttgccgt
     1621 gcgcctggtt aattcccatc atatcatcta tgaggacgcg ctcgttctcg tcatcaatgc
     1681 gatagaacgc ggtgtgtgtc atcgatatgt gaaggcgata cgtgtgtttt ttagagccct
     1741 tgatgagttt cttgttgcat cgcctggttg cctttttcag gaactctcga agacacaaag
     1801 agatgacctc tctcacatgt gccaggtctt tttttggtat ttgcgatagg tatttgagcg
     1861 cttcctcact gaatatgagc tcatacacct ttgaaaacct cttcgaaggt gtaaaactta
     1921 gacttcttct cgattatatc cagatcggca tccagtttct caacatccca gttttcatcg
     1981 tcgaccatct gggagatcac atcatcatac gtcatatttc ccttcttcaa cttcttcaac
     2041 ttctcctggg tcgcgcgact cacacggata ctcaccatat cagagttatc tggacttacg
     2101 ctctgggtca taaatagtga tgatcactgc gataacatta tgtttttgct ggcgatgcgg
     2161 ttaccgacat aagccggggg ccatggtggc cggcgagaag cctctatcca cggggagtgt
     2221 ttacggatcg agcaacgcac ctcccgggca gcctcaaccg aagagagcgc cggcggcgac
     2281 tcaccaggga gatgacc
//
LOCUS       DAOSII010000247         2286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_40667_length_2286_cov_51.251905, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000247 DAOSII010000000
VERSION     DAOSII010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40667_length_2286_cov_51.251905"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="PLV90_10900"
     CDS             complement(<1..666)
                     /locus_tag="PLV90_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HOF97664.1"
                     /translation="MNGYWDGPKPEPQARTIDEMREVLADPGCTFDQPLYFMYRDLAK
                     NESDRAWLRQQHIRYDITVIPAATLCGEHVKTMGHYHTLNPAGVFYPEIYEVISGTGH
                     YLLQTRSADDVVMIRGIAGDKILIPPGYGHVTINPGKVDLVMANLVSAEFSSNYGPFA
                     EKRGAAYYETEGGALVKNPHYPDASPVRYCDPTEVPELGIEKGTGLYDLIGRPRSVAF
                     LNHP"
     gene            complement(666..1943)
                     /locus_tag="PLV90_10905"
     CDS             complement(666..1943)
                     /locus_tag="PLV90_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HOF97665.1"
                     /translation="MRWQHVPVLMLLLALLAVPGSAAGAKYLYGNPELSATIAGTNEF
                     APGAETGLTVIISNSGLNTHKIAIPSLPPDDLPNTAKLVTVALKSNDAPFTVKTDPQF
                     LSDIMGGAALPATFNVKVADSAAPGTYTLPLEVTYTYLQDAEDYGDLLRYNYQKKTET
                     LPLTVRVTPGLRVEVLNVQTRSLNVGTEGYVSMTLKNIGHDAADKAVAKVARNGASPL
                     IPTDSNAYIGTFAPGETVDVKFKVAVADSAEPQSYPLDIVVAYEDYEGKSTASRPVTI
                     GLPVGGKIDFDVASPTVTLHPGGKSILEVVYRNAGAAKVYSAQARISAVDPFTSSDDT
                     AYLGDLAPGETATARFEVSIDADATLKNYGIDSEIRYRDDLDNSKISDTMKVQVALEK
                     KEGTLFTNPVFLAVIAVVIIGAGYYIFAYRKKK"
     gene            2007..>2286
                     /locus_tag="PLV90_10910"
     CDS             2007..>2286
                     /locus_tag="PLV90_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOF97666.1"
                     /translation="MTAPRDIPNDLIESLKSLGLTRYEALVYIGLLRATGATATEVHE
                     TSGVPRASVYPVLDRLVQKELVSVSHTTPKRFDAVPPGQGIENLMRRIE"
BASE COUNT          393 a          673 c          717 g          503 t
ORIGIN      
        1 ggggtggttg aggaaggcga ccgaccgggg gcggccgatc aggtcgtaga gcccggtgcc
       61 cttctcgata ccgagttcgg gaacctctgt cgggtcgcag tagcgcaccg gcgacgcgtc
      121 ggggtagtgc gggtttttca cgagcgcccc tccctccgtc tcgtagtagg cagcgccccg
      181 tttttcggca aaaggaccgt agttgcttga gaactccgcc gagacgaggt ttgccatcac
      241 aagatcgacc tttccggggt tgatggtcac gtggccgtac cccggcggga tcaggatctt
      301 atccccggcg atgccccgga tcatcaccac gtcgtcggca cttctggtct ggaggaggta
      361 gtgccccgtc cccgagatca cctcgtagat ctccgggtag aagaccccgg ccgggtttaa
      421 tgtgtggtag tgtcccatcg tcttgacgtg ctccccgcag agggtcgccg ctggaatgac
      481 ggtgatgtcg tagcggatgt gctgctgtcg gagccacgcc cggtcgctct cgtttttggc
      541 gaggtcgcgg tacataaaat agaggggttg atcgaaggtg cagccgggat ctgcgagaac
      601 ctcccgcatc tcatcgatcg tccttgcctg cggttccggt ttgggcccgt cccagtatcc
      661 gttcatcact ttttcttacg gtacgcgaat atataatacc ctgcaccgat gatgacgacc
      721 gcaattacag cgaggaatac cggattggtg aagagggtgc cttctttctt ctcgagtgcg
      781 acctgcacct tcatggtatc cgatatcttg ctgttgtcaa ggtcgtcgcg gtaacggatc
      841 tcggagtcga taccgtaatt cttcagggtc gcatcggcat cgatgctcac ctcaaaccgt
      901 gccgtcgcgg tctcgccggg agcaaggtcg cccaggtagg cggtgtcgtc gctgctggtg
      961 aaggggtcga cggcgctgat ccgggcctga gcactgtaga cctttgccgc ccctgcgttt
     1021 ctgtacacga cctcaaggat gctcttgccg cccgggtgga gcgtcaccgt cggcgaggcg
     1081 acatcaaagt ctatcttgcc gccgaccgga aggccgatcg tcaccggccg ggaggccgtt
     1141 gacttcccct cgtagtcctc gtaggcgacc acgatgtcca gcggataaga ctggggttcg
     1201 gcgctatcgg cgaccgcgac cttgaacttt acatccaccg tctcccctgg cgcaaacgtc
     1261 ccgatgtagg cgttgctgtc ggtggggatg agcgggcttg cgccgtttct cgcgactttc
     1321 gcgaccgcct tatctgctgc gtcgtgcccg atgttcttca aggtcataga gacgtagcct
     1381 tccgtgccga cgtttaacga tcgggtctgc acgttcagga cctcgacgcg gaggccaggc
     1441 gtgacccgga cggtcagggg cagggtctcg gtcttcttct ggtaattgta gcggaggagg
     1501 tcgccataat cctctgcatc ctgcaggtag gtgtaggtca cctcaagcgg gagggtgtag
     1561 gtgcccggcg ctgccgagtc tgccaccttc acgttgaacg tggcgggcag ggctgcgccc
     1621 cccatgatgt cgcttaagaa ctgggggtcc gtcttgacgg tgaacggggc atcgttgctc
     1681 ttgagcgcta cggtgaccag cttcgcggta ttcggcaggt cgtcgggggg gagcgacgga
     1741 atggcgatct tatgagtatt caagccgcta ttcgatatga tgaccgtcag gccggtctcg
     1801 gcgcccggtg caaactcatt ggtgccggcg atcgttgccg aaagttctgg gtttccatag
     1861 aggtacttgg cccccgccgc cgaccccggc acagccagga gtgccagcag gagcatcagt
     1921 accggtacgt gctgccaacg catctcttca ccttgttgtt attttagcaa caacatttat
     1981 tatcaggccg tatatatatg tgatggatga cggctccccg ggatattcca aacgacctca
     2041 tcgaatctct caagtcgctt gggcttacaa ggtatgaggc cctcgtctac atcggcctcc
     2101 tcagggcgac gggtgcgacg gcgacagagg tccacgagac gtccggggtg ccgcgcgcgt
     2161 ccgtctaccc ggtcctcgac cggctcgtcc agaaagaact ggtcagcgtc tctcacacca
     2221 cgcccaaacg gttcgacgcg gttccgccgg gccagggtat cgagaacctc atgcgccgga
     2281 tcgagg
//
LOCUS       DAOSII010000248         2266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_41146_length_2266_cov_54.862958, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000248 DAOSII010000000
VERSION     DAOSII010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41146_length_2266_cov_54.862958"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PLV90_10915"
     CDS             <1..663
                     /locus_tag="PLV90_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HOF97667.1"
                     /translation="PPGEAKEDWQIISELAARMGYAAQFPYKDIEEIFDEVAVLTPSY
                     HGMNYERLNRPEALHWPCPDASHPGTPILHREKFSHPDGIGIFAPIEWKPPAEVPDAE
                     YPFVLTTGRVLWHWHTGSMTRRSATLDAEVPTGWIEINPEDAKALGIKDKEKVRAVTR
                     RGSVDVPAKVTPDIMKGVMFMPFHFAECAANILTNNALDPIAKIPEFKACAVKVEKIT
                     EA"
     gene            666..1817
                     /locus_tag="PLV90_10920"
     CDS             666..1817
                     /locus_tag="PLV90_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOF97668.1"
                     /translation="MAAIGDMFYTWAADADVQKRGECGGAITALQQYALKSGMVDAVL
                     AVRKGADIYDAVPTVITDPAEVAETAGSLHCGTLLLSKLVKNYLDSNKTARLGVTVKG
                     CDTMGLIELAKRNQVDLDRVILLGVNCGGSVSPVLARKMIREKFEVDPDIVHKEEIDK
                     GQFIIEYEGGHKGIKIDDLEDEGYGRRSNCRRCLYKVPRQADLACGNWGVIGEKAGKA
                     TFVEVCSEKGANLLDAAIKAGAVMTEPANPKGIEIRGKVEGAMLNLGNKWRKKDFGAL
                     ASDLWGTISRETGRCIKCYSCIENCPVCFPVAKDFKGGSRMIASGEVPPNPMFHLRRF
                     THISDSCINCGQCEELCPMEIPLALFSHAIRTEGDSAFESKLGKAPYSN"
     gene            2026..>2266
                     /locus_tag="PLV90_10925"
     CDS             2026..>2266
                     /locus_tag="PLV90_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HOF97669.1"
                     /translation="MELKYVQTTCPYCGTGCSFNLVVKDGKVVGTQPYTRSPVNEGKV
                     CPKGTYAHEFVNSPDRLTKPLIKKDGKFVEATWDEA"
BASE COUNT          501 a          705 c          671 g          389 t
ORIGIN      
        1 ccgccgggcg aagcaaagga agactggcag atcatcagcg agcttgccgc ccggatgggc
       61 tacgccgccc agttccccta caaggacatc gaggagatct tcgatgaagt cgccgttctc
      121 acgccatctt accacggcat gaactacgag cggctgaaca ggcccgaggc cctccactgg
      181 ccctgccctg acgccagcca cccgggaacg ccgatcctgc accgcgagaa gttctcccac
      241 cccgacggca tcggcatatt cgccccgatc gagtggaaac cgccggcgga agtccccgat
      301 gccgagtacc cgttcgtcct cacgaccggg cgcgtcctct ggcactggca caccggcagc
      361 atgacccgcc ggtccgcgac gcttgacgcc gaagtcccca cgggctggat cgagatcaac
      421 cccgaggacg caaaagcgct cggcatcaag gacaaagaga aggtccgtgc cgtcacccgc
      481 cgtggctcgg tcgacgtccc cgcgaaggtg acccccgaca tcatgaaggg tgtcatgttc
      541 atgccgttcc acttcgccga gtgtgcggca aacatactga cgaacaacgc tctcgatccc
      601 atagcaaaga tcccggagtt caaggcctgt gctgtgaagg tcgagaagat tacggaggcc
      661 tgaagatggc agcaattggc gatatgttct acacgtgggc ggcagacgcc gacgtgcaga
      721 agagaggcga atgcggaggc gcgatcaccg ccctgcagca gtatgccctt aagtccggca
      781 tggtggacgc ggtccttgcc gtccggaagg gcgcggacat ctacgatgcg gtcccgaccg
      841 tcatcaccga ccccgccgag gtcgcagaga cggcaggctc gctccactgc ggaacgctcc
      901 tgctctcgaa gctcgtcaag aactacctgg acagcaacaa gaccgcacgg ctcggtgtca
      961 ccgtcaaggg ctgcgacacg atgggcctca tcgagcttgc gaagcgcaac caggtcgacc
     1021 tggaccgcgt catcctgctc ggcgtgaact gcggcggctc ggtcagcccg gtcctggccc
     1081 ggaagatgat ccgcgagaag ttcgaggtcg atcccgacat cgtccacaaa gaggagatcg
     1141 acaagggcca gttcatcatc gagtacgagg gtgggcacaa agggatcaag atcgacgacc
     1201 tcgaggacga gggttacggc cgccgttcga actgccgccg gtgcctctac aaggtcccgc
     1261 gccaggctga cctcgcctgc gggaactggg gcgtcatcgg cgagaaggcc gggaaggcga
     1321 cgttcgtcga ggtctgctcc gagaagggtg cgaacctcct cgatgccgcc atcaaggccg
     1381 gtgccgtcat gaccgaaccc gcaaacccga aggggatcga gatccgcggc aaggtcgagg
     1441 gggccatgct caacctcggc aacaagtggc ggaagaagga cttcggggcg ctcgcatcgg
     1501 acctctgggg caccatcagc cgcgagacgg gccggtgcat caagtgctac tcctgcatcg
     1561 agaactgccc cgtctgcttc ccggttgcaa aggacttcaa gggaggctcg aggatgatcg
     1621 catccggcga ggttccgcca aacccgatgt tccacctccg gcggtttaca cacatatccg
     1681 actcgtgcat caactgcggc cagtgcgagg aactctgccc gatggagatt ccgcttgcac
     1741 tcttctccca tgccataagg accgagggcg acagcgcatt cgagtcgaag ctcgggaagg
     1801 caccctactc caactaattt ttttagcccg ccgtacgggc agcgcatcat tactcagaat
     1861 agcgatgatc ccccgaatcg gtattcaggt ggttataccc ggtattgtat gtttttccag
     1921 aaaatcggtg tggtgcaacc cacacttatt catcaaaaat gggggtgtta atcactacta
     1981 ttaaatatga ttgacctcgt attcttacat atccgaggtt ttaccatgga actcaagtat
     2041 gtacagacga catgcccgta ctgtggtacg ggatgcagct tcaacctcgt cgtgaaggac
     2101 gggaaggttg tcggcacaca accttacacc cgttcgcccg tcaacgaggg aaaggtatgc
     2161 ccgaagggca cctacgctca cgagttcgtg aacagcccgg accgcctcac aaagccgctc
     2221 atcaagaagg acggcaagtt tgtcgaggcg acctgggatg aggcct
//
LOCUS       DAOSII010000249         2250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_41550_length_2250_cov_45.156264, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000249 DAOSII010000000
VERSION     DAOSII010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41550_length_2250_cov_45.156264"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /gene="moaA"
                     /locus_tag="PLV90_10930"
     CDS             complement(<1..701)
                     /gene="moaA"
                     /locus_tag="PLV90_10930"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HOF97670.1"
                     /translation="MALKDPYNRTVTNLRISLTSRCNLRCIYCHAEGEVNPREQMSAA
                     DIAELMQVGVKFGIKSVKFTGGEPLLRRDLVDIVRSVPPGVESSMTTNGTLLAEKAAA
                     LKEAGLARVNVSLDTLRPERYAAITGKDCLADVLAGIDAAVEVGLTPVKLNMVLLEGI
                     NEDELDDFMAFVRSKRDLILQVIELMEFNECKFHGDVDSVEQELNERATRVVTRRMHH
                     RKKYCLDGAEVEVVRP"
     gene            complement(679..1290)
                     /locus_tag="PLV90_10935"
     CDS             complement(679..1290)
                     /locus_tag="PLV90_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HOF97671.1"
                     /translation="MGSQWTKDSVYRKAMKAGYRARAAYKLLEIQRRNEIIRPDDNVV
                     DLGAAPGSWLQVIRELTDGKVIGVDLNPIAPMEGVTTIIGDFTDPLVQERIREEAGGI
                     VNIVVSDAAPKLSGQKSYDQARAIGLGEDALAFACTILKPGGNLVIKSFQGELFGDLI
                     AAMKQHFYSVRGYRTKASRKGSAETYIIAKNFKGKCDGVEGPL"
     gene            1491..2234
                     /locus_tag="PLV90_10940"
     CDS             1491..2234
                     /locus_tag="PLV90_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase sliding clamp"
                     /protein_id="HOF97672.1"
                     /translation="MIKATIDADIFRESIDAIAALVTECRLHTADDHTWTRAVDTANV
                     AMVSLDLRSTAFNSFSATAGELGLDITKMKNILGMMGKGDELTLSLLDEERKLELSFG
                     GYRYSVTLLDTNTIRKDPNPPGLELPGKAVISGDALNNAIKAAAVISDKIALGIDPDA
                     MTFFMEAEGDTDHIKLALGEDQLVALNPVRARSLFSLDYLKDMGRVMARADEVEVYLG
                     IDHPVRFAFDIAGGNGHVEYLLAPRIEAD"
BASE COUNT          443 a          704 c          657 g          446 t
ORIGIN      
        1 ggccggacca cctcgacctc ggcgccgtcg aggcagtact tcttccggtg gtgcatccgg
       61 cgggtgacga cccgggttgc ccgctcgttc aactcctgct cgacgctgtc cacgtccccg
      121 tggaacttgc actcgttgaa ctccatcaac tcgatgacct gcaggatgag gtcccgcttc
      181 gaccggacga acgccatgaa gtcgtccagt tcatcctcgt tgatgccctc aaggagcacc
      241 atattcaact tgaccggggt caggccgacc tcgaccgccg catcgatgcc ggcaaggaca
      301 tccgcgaggc aatctttccc cgtgatggcc gcataacgct cggggcggag cgtatcgagg
      361 ctgacgttca cccgggcaag ccccgcctcc ttgagcgccg cagccttttc ggcaaggagc
      421 gtcccgttcg tcgtcatcga ggactcgacg cccggcggca cggagcggac gatatcgacg
      481 aggtcacggc ggagcagggg ttctccgccg gtgaacttga cgctcttgat accgaacttc
      541 acgcccacct gcatcaactc ggcgatatcc gcagcgctca tctgctctct tgggttcacc
      601 tcgccctcgg catgacagta gatgcaccgc aggttgcacc gggaggtcag gctgatccgg
      661 aggttggtca cggtccgatt ataggggtcc ttcaacgcca tcgcactttc ccttgaagtt
      721 ttttgcaata atgtaggtct cggcactccc ttttcgtgac gccttcgtcc ggtacccgcg
      781 gacggagtag aagtgctgct tcatcgcggc gatcaggtca ccgaagagtt ccccctggaa
      841 cgactttatc accagattgc cgccgggctt taagatcgtg caggcaaacg ccagggcatc
      901 ctcgccaagc ccgatcgccc gggcctggtc gtaactcttc tggccggaga gtttcggcgc
      961 ggcgtcggag accacgatgt tgacgattcc gcctgcctcc tcgcggatac gctcctggac
     1021 gaggggatcg gtgaagtccc cgatgatggt ggtgacgcct tccatgggcg cgatggggtt
     1081 gaggtccacg ccgataacct tcccgtcggt cagctcgcgg atcacctgca gccagctccc
     1141 cggcgccgcc ccgagatcga cgacgttgtc gtccggccgt atgatctcgt tgcgccgctg
     1201 gatctccagt agtttgtagg ctgcccgggc tcggtacccg gccttcatcg cctttctata
     1261 gacactgtct tttgtccatt gcgaacccat tgcgagactg ccctccgctt gaggccggca
     1321 ggcggtacgt gcgctgccgg gcacgatcag gagacgatat acaatgtttg atgcatgtga
     1381 ccatgaaggt ataggattcc ggcatacggg atacgccggg gcacatagca aagcactaaa
     1441 atggatagga cgattatata cgttagacga gagtttgata aggggtcaca atgataaagg
     1501 caacgattga tgcagatata tttcgggaat ccatcgacgc gatcgccgca ctggtgacgg
     1561 agtgccgcct gcacacggca gacgatcaca cctggacacg agccgtcgac accgcaaacg
     1621 tggccatggt ctccttagac ctccggagca cggcgttcaa ctccttctcg gcgacggccg
     1681 gagaactggg cctggacatc accaagatga agaacatcct cggcatgatg ggaaagggcg
     1741 acgaactgac gctcagcctg ctcgacgagg agcggaagct cgagctgagc ttcgggggct
     1801 accgctattc ggtcacgctg cttgacacca acaccatccg gaaagaccca aaccccccgg
     1861 ggcttgaact ccccggcaag gcggtcatat cgggagacgc actgaacaac gccatcaaag
     1921 cggccgcagt catatccgat aagatagctc tcggtatcga ccccgacgcc atgacctttt
     1981 tcatggaggc ggagggagat accgaccaca tcaaactcgc gctcggtgaa gatcagctcg
     2041 tcgcactcaa cccggtgcgg gcccgctccc tcttctcgct cgactacctc aaagatatgg
     2101 gccgggtcat ggcacgcgcg gatgaggtgg aggtctacct cggcatcgat cacccggtac
     2161 ggttcgcctt cgatatcgcg ggcggcaacg gccatgtcga gtacctcctt gctcctcgga
     2221 tcgaggccga ttgatggatg tcgtactcga
//
LOCUS       DAOSII010000250         2226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_42184_length_2226_cov_72.089820, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000250 DAOSII010000000
VERSION     DAOSII010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42184_length_2226_cov_72.089820"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="PLV90_10945"
     CDS             complement(<1..1238)
                     /locus_tag="PLV90_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minichromosome maintenance protein MCM"
                     /protein_id="HOF97673.1"
                     /translation="MPEEEVTVEVTDVVGEWTKILKKQFKRELAELSREYPHKRSLII
                     DFRKILNNRLAFELLRSPGKVLGDIRDAIVQNRLLKLKDGQDPDLINIRFTNLPQKTD
                     VRDIRADQINTFIGIEGILRKTTEVRPRIVSAVFRCRSCGKNTDPVPQGYGRFDEPDV
                     CPNCERKTRLDLVMNRCRFVDAQKLRIQESPEGLRGGEQPQTLDIDVTDDLTGMVSPG
                     DRVVVNGILRSVQRVNYGQKSTLFDIYLECNSIEVAEKEFEEVSITEEDEVKIKALAR
                     DPLVYKKIARSIAPTIYGTDDVKEAIALQLFGGIAKEMPDGSRLRGDVHVLLVGDPGI
                     AKSQILRYVVKLSPRGIYTSGKSSTSAGLTATAVKDEFGDGRWTLEAGALVLADMGIA
                     AVDEMDKMAKEDRSALHEAM"
     gene            complement(1251..2219)
                     /locus_tag="PLV90_10950"
     CDS             complement(1251..2219)
                     /locus_tag="PLV90_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C small subunit"
                     /protein_id="HOF97674.1"
                     /translation="MDGNHTIWIEKYRPRILDEMVGQKDIVVRLQSYVKTGNLPHLLF
                     TGSAGIGKTTAAVALAREFFGDSWQTNFREMNASDERGIDVVRNQIKQFARTSPLAGA
                     KFKILFLDEADALTTDAQAALRRTMETYARTCRFILSCNYSSKIIDPIQSRCAIYRFR
                     PLDREAVIEETRRIAAVEGLTITEGALDAIVYVASGDMRKAINALQGAAILRPDIDED
                     LIYEITATARPAEIDELIDLSLAGKFDEAEQALSELTRGRGIAPNELINQCYRALVRR
                     DIDRTLKVRLIDALGETDFRLSEGASSDIQMEALLARFVLAAGEHR"
BASE COUNT          350 a          715 c          702 g          459 t
ORIGIN      
        1 tccatcgcct cgtggagcgc gctccggtcc tccttcgcca tcttgtccat ctcgtcgacg
       61 gcggcgatcc ccatatccgc aaggaccagc gcgccggcct cgagcgtcca gcgcccgtcg
      121 ccgaactcgt ctttcacggc cgtcgccgtc aggccggcgg atgtcgacga cttgccgctc
      181 gtgtagatcc cgcggggcga gagcttcacg acgtagcgca atatctggct cttggcgatg
      241 cccgggtcgc cgaccaggag gacgtggacg tcgccgcgga ggcgggagcc gtccggcatc
      301 tcctttgcga taccgccgaa gagctggagc gcgatcgcct ccttcacatc gtcggtcccg
      361 tagatcgtcg gcgcgatcga ccgggcgatc ttcttgtaga ccaggggatc gcgggcgagc
      421 gccttgatct tcacctcatc ctcctcggtg atcgagacct cttcaaactc cttctcggca
      481 acctcgatcg agttgcactc caggtagata tcaaagagcg tgctcttctg gccgtaattg
      541 accctctgca ccgaccgcag gatgccgttc accaccaccc ggtcgcccgg cgagaccatc
      601 ccggtgaggt cgtcggtgac gtcgatatcg agcgtctggg gctgctcgcc accgcggagg
      661 ccttcgggag actcctggat ccggagtttc tgggcatcga cgaaccggca gcggttcatg
      721 acgagatcga gtcgggtctt cctctcgcag ttcgggcaga cgtcgggctc gtcgaaccgc
      781 ccgtagccct gggggaccgg gtcggtgttc ttcccgcagg agcggcaccg gaagaccgcg
      841 ctcacgatcc gggggcggac ctcggtcgtc ttcctgagga tcccctcgat gccgatgaac
      901 gtgttgatct ggtcggccct gatatcccgg acgtcggtct tctgcggcag gttcgtgaac
      961 cggatgttga tcaggtccgg atcctggcca tctttgagtt tgaggagcct gttctggacg
     1021 atcgcatccc ggatgtcgcc gaggaccttc cccgggctcc gcaggagctc aaacgcgagc
     1081 ctgttattca ggatctttcg aaaatcgatg atgagcgacc gcttgtgcgg gtactcgcgg
     1141 gagagctcgg cgagctcccg cttgaactgt ttttttagga tcttcgtcca ctcaccgacg
     1201 acatcggtga cctcgacggt tacctcttct tcaggcacgg ccggagcccc tcaccggtgc
     1261 tctcctgcgg cgaggacgaa ccgggcgagc agcgcctcca tctggatatc gctgcttgcc
     1321 ccttccgaga gacggaagtc cgtctcgccc agggcatcga tcagtctcac cttgagcgtc
     1381 cggtcgatat cgcgccggac aagcgcccgg tagcactggt tgatcagctc gttcggggcg
     1441 atgccccggc cgcgggtcag ttcggagagc gcctgctcgg cctcatcgaa cttccctgca
     1501 agcgagaggt cgatgagctc gtcgatctcc gccgggcggg cggtcgcggt gatctcgtag
     1561 atcagatcct cgtcgatatc gggccggagg atggcggcac cctgcagggc gttgatcgcc
     1621 tttcgcatat ccccggaagc gacatagacg atggcgtcga gcgccccttc cgtgatggtg
     1681 aggccttcga ctgcggcgat cctccgggtc tcctcgatga ccgcctcgcg gtcgagcggc
     1741 ctgaaccggt atatggcgca ccggctctgg atgggatcga ttatctttga agagtagttg
     1801 cacgagagga tgaaccggca ggtgcgggcg taggtctcca tcgtcctccg gagggcggcc
     1861 tgcgcatccg tcgtgagcgc atccgcctca tccaggaaga ggatcttgaa cttcgcgccg
     1921 gcaagcggcg atgtccgggc gaactgtttg atctggttcc tgacgacgtc gatgccgcgc
     1981 tcgtcggatg cgttcatctc ccggaagttc gtctgccagg agtcgccgaa gaactcccgc
     2041 gcgagcgcca cggcagcggt cgtcttccct atcccggcgc tcccggtgaa gaggaggtgc
     2101 ggcaggttgc cggtcttcac gtaggattgg aggcgcacca cgatatcctt ctgcccgacc
     2161 atctcgtcaa gtatccgggg ccgatacttc tctatccaga tggtgtggtt accgtccatt
     2221 tgcatg
//
LOCUS       DAOSII010000251         2226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_42185_length_2226_cov_58.949332, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000251 DAOSII010000000
VERSION     DAOSII010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42185_length_2226_cov_58.949332"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLV90_10955"
     CDS             complement(<1..485)
                     /locus_tag="PLV90_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOF97675.1"
                     /translation="MRLARRSTQTTIFGEFVPTFPKVRTSRGYLLDWDRNRIIRQIVE
                     ETRLVEVFYGYEGADEETAKDIARRVERKIQMLGLQSLSGPLIREIVNMTLLERGLIS
                     YRNVCTRVGTPVFDAHLIDVGRGFEAHDNANLQENAETSHKKKADKISKEQYLLQLPP
                     DL"
     gene            complement(482..751)
                     /locus_tag="PLV90_10960"
     CDS             complement(482..751)
                     /locus_tag="PLV90_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOF97676.1"
                     /translation="MSDTAQFIVYTLEFCPNCEILKEFLSSRGASFIECDMASAEALT
                     ELRMGGIFVREAPVLQKGEEFYTSADLFAGGAFREDLVADLIAEA"
     gene            972..1511
                     /gene="thpR"
                     /locus_tag="PLV90_10965"
     CDS             972..1511
                     /gene="thpR"
                     /locus_tag="PLV90_10965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOF97677.1"
                     /translation="MVRTFVAIDLPEAIRERARASQAALARAEGRLAIVDPANLHVTV
                     KFLGEVDPAQIGPIGEALGAVTADPFDLTVGRAVCNPPRRARVIWCDVIDAGESAAIA
                     RQVDDLLSPLGFSRENRPFRPHVTLARVKEFHPSQCREVASLPPEPLGRCRVASIKLK
                     KSTLTPRGSIYEDLLEVPL"
     gene            1508..>2226
                     /gene="cca"
                     /locus_tag="PLV90_10970"
     CDS             1508..>2226
                     /gene="cca"
                     /locus_tag="PLV90_10970"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOF97678.1"
                     /translation="MTRCPCEEEVLRRIRPTPEERAYIRTIGERLIEAVERSGMAKAM
                     MVGSVARDTFVRGDRDLDVFMLFDPALSREALQEEGLSLAHRVAEEFGATWREKYAEH
                     PYVNATINSLDIDLVPCYAVASATDIKSAVDRTPFHTRYILAHIDGYADDVLLAKQFA
                     KAGGVYGSDHMTEGFSGYLCEILTIYYGGFHALLRAAACWRPGEVVDIEGHGRKPFDE
                     PLVVIDPVDPERNVAAALSLSR"
BASE COUNT          377 a          722 c          703 g          424 t
ORIGIN      
        1 agatcgggag ggagctgcag gaggtactgc tccttgctga tcttgtccgc cttctttttg
       61 tgcgacgtct cggcattctc ctggaggttc gcgttgtcgt gagcctcaaa tccccgcccg
      121 acgtcgatga ggtgcgcgtc aaagaccggg gttcctaccc gggtgcagac gttgcggtag
      181 gatatgaggc cgcgctcaag gagcgtcatg ttgacgatct cgcggatcag ggggccggag
      241 agcgactgca gcccgagcat ctggatcttt ctctcgaccc ggcgggcgat atccttcgcc
      301 gtctcctcgt cggccccttc gtagccgtag aagacctcga cgaggcgggt ctcctcaacg
      361 atctgccgga tgatccggtt cctgtcccag tccaggaggt agccccgcga tgtcctgact
      421 ttcgggaagg tggggacgaa ctccccgaat atagtcgtct gggtcgacct tcgcgccaac
      481 ctcatgcctc cgcgatcagg tcggcgacga ggtcttcccg gaacgcgccg ccggcaaaga
      541 ggtcggccga tgtgtagaac tcttcgccct tctgcagcac cggcgcctcc cggacgaata
      601 tcccgcccat gcgaagttcg gtcagcgcct cggcggacgc catgtcgcac tcgatgaagg
      661 aagcacccct ggaggagagg aactctttga gaatctcgca gtttgggcag aattccagtg
      721 tatagactat aaattgcgct gtatcggaaa tggtaatacc cccggagtcg gtcgttatgt
      781 tttcgttctg tacggatata acaggtacct ttcttccgaa caggcctctc tctcccggtg
      841 agcgtgtgtc ccgggcgtta cgggggatcg ccgggaactc acccctgcgc cgctcgatat
      901 cccggctccg tcgggtacgc tcctgtcgta caatcaccat tttgtaggga gaggcacgat
      961 agtacggtag tatggtcagg acgttcgttg caatcgacct gcccgaagcg atccgcgaga
     1021 gggcgcgtgc gtcccaggcg gctctggcac gggccgaagg aaggctcgcc atcgtcgacc
     1081 ccgcaaacct tcacgtcacg gtgaagttcc tcggagaggt cgatcctgca cagattgggc
     1141 ctatcggcga ggcgctcggg gccgtcacgg ccgacccttt cgacctgacg gtcgggcgtg
     1201 cggtctgcaa ccccccgcgg agggcgcggg tgatctggtg cgacgtcatc gacgccggag
     1261 agagcgccgc tattgcccgg caggtggacg acctcctctc gccgctcggg ttttcccggg
     1321 agaaccgccc ttttcgcccc cacgtcaccc ttgcgcgggt caaggagttc cacccctcgc
     1381 agtgccggga ggtagcctcc ctgccccctg aaccgctcgg gcggtgccgc gtcgcgagca
     1441 tcaagctcaa gaagagcacg ctcacccccc gggggtccat ctacgaggat cttctggagg
     1501 tcccgctttg acccggtgcc cctgtgagga ggaagtgctc cggcggatcc gcccgacacc
     1561 cgaggagcgg gcatacatcc gtacgatagg agagcgcctg atcgaggcgg tggagcggtc
     1621 aggtatggca aaggcgatga tggtgggctc ggtcgcccgc gacaccttcg tccggggcga
     1681 ccgggacctc gatgtcttca tgctctttga ccccgccctc tcccgcgagg ccctccagga
     1741 ggaaggcctc tccctcgcgc accgggtcgc ggaggagttc ggcgcgacct ggcgcgagaa
     1801 gtatgccgag cacccctacg tcaacgcgac gatcaactcg ctcgacatcg acctcgttcc
     1861 ctgctacgcc gtcgcaagcg ccaccgatat caagagcgcc gtggaccgga caccgtttca
     1921 cacccgctac atcctcgccc acatcgacgg ctacgccgac gacgtccttt tagccaagca
     1981 gtttgcaaag gcgggcgggg tctacgggtc ggaccacatg accgaggggt tctccggcta
     2041 cctctgcgag atcctgacga tctactacgg cggtttccat gcgctcctcc gcgccgccgc
     2101 ctgctggagg ccgggcgagg tggtcgatat cgaggggcac gggagaaagc cgtttgacga
     2161 accgctcgtc gtcatcgatc cggtcgaccc ggagaggaac gtggcggcgg cgctctcgct
     2221 ctcgcg
//
LOCUS       DAOSII010000252         2212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_42529_length_2212_cov_54.738526, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000252 DAOSII010000000
VERSION     DAOSII010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42529_length_2212_cov_54.738526"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            374..1144
                     /locus_tag="PLV90_10975"
     CDS             374..1144
                     /locus_tag="PLV90_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97679.1"
                     /translation="MEPVERVFARDFAAARHTVDSEGGRGGSYVVTPGGAWCRRLFIV
                     GALTERRGSGDVMQARVADPTGTFRVSMDRQTPDAGETLGYIEPPAFVAVTGRATIVG
                     SGPRAYATVDAEALRVVDRTARDLWVQKTAGTTLDRLIALGEALSGDGDERAATTLRL
                     YGTTEEAIRDMAGMVRTALAVVRPGTVPGDIGFVPRRDILLDALEEEGGTRGVPVEDA
                     VAAGVRSGLSAREARAALEELLAAGECYQPASGMVKLI"
     gene            1151..1906
                     /locus_tag="PLV90_10980"
     CDS             1151..1906
                     /locus_tag="PLV90_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOF97680.1"
                     /translation="MHLHFIESGPALLVEQDRRVLVVADLHMGIESGLERHGVHIASR
                     SAVRIARVVACIEEMKPDLLLLLGDVKHNVPVTSRQEYRELPGTLATLRERVPLAVVP
                     GNHDGGIGRFLEPGELLPPGGTVIDGVGYLHGHTRPDPELFGRLIILGHHHPVVSLVD
                     AVGCAARARPAYLYSGLDAGCEVETPPGGNRTRLLFIPAFNEFAGGIDVGRLSESGLG
                     PLSRCIERETAEVFLADGTYVGSPATLSPAGGG"
     gene            1854..>2212
                     /locus_tag="PLV90_10985"
     CDS             1854..>2212
                     /locus_tag="PLV90_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOF97681.1"
                     /translation="MVRTSAPRPRSPPPAAGELLSPAVREVVRKRGFTALSDAQEQAI
                     PLLLEGKNLVLVAPTGTGKTESAMLPVFDRLLATAGPGFKAVYVTPLRSLNRDILARM
                     EWWCRELSLSAGVRHGDT"
BASE COUNT          345 a          752 c          731 g          384 t
ORIGIN      
        1 tatccggggc cgatacttct ctatccagat ggtgtggtta ccgtccattt gcatgaagtg
       61 ttggcgctcg cgcattgtaa aagcatcgac atcgcctccg gtgatccttt ttagagggtt
      121 gagagagaaa ggataggtac agattactgt ggagataccg ggagttcgcg tggagatccg
      181 gtcttcgata aggcccgata aacccgcgca tccctggttt cccccttcac ggcagcgcga
      241 gcggccgcgc cggcatcccc gctcaatcgc ttcgcctgct gcctcaaaga ctgcagggtc
      301 atgacgaccc tcctcaccga tcgatgccct cgcatcaggg cgactcgacg cactcaggga
      361 atagacggct gatatggaac ctgtagaacg ggtatttgcg cgagactttg ccgccgcgcg
      421 gcatacggta gactccgagg ggggccgtgg cggctcgtac gtcgtgacgc ccggcggcgc
      481 ctggtgccgc cgcctcttca tcgtcggcgc cctcaccgag cgcaggggga gcggtgatgt
      541 catgcaggcc cgggtcgccg acccgaccgg cacgttcagg gtctcgatgg accggcagac
      601 cccggacgcg ggcgagacgc tcgggtacat cgaaccgccg gcgttcgtcg ccgtcaccgg
      661 ccgggcgacg atcgtcggtt ccgggccgag ggcgtacgca acggtggatg ccgaggcgct
      721 ccgcgtggtc gaccgcaccg cccgcgatct ctgggtgcaa aagaccgccg gcacaacgct
      781 cgaccgcctc atagcgctcg gagaagctct atcgggcgac ggtgacgagc gggctgcaac
      841 caccctccgg ctctatggga cgacggagga agcgatccgg gatatggccg ggatggtgcg
      901 cacggcgctc gcggtcgtcc gcccgggtac cgttcccggc gatatcggct tcgtcccccg
      961 gcgcgacatc ctccttgatg cgctcgagga ggagggcggc acccggggtg tccccgtcga
     1021 ggatgccgtc gccgccgggg tgcgttccgg cctctccgcg cgggaggcac gtgcggcgct
     1081 tgaggaactc cttgctgcag gcgagtgcta ccagcctgca tcgggcatgg taaaactgat
     1141 ataacacgtt atgcacctgc actttatcga gagcggtcct gccctcctcg tcgagcagga
     1201 ccggcgcgtg ctcgtcgtcg ccgatctgca catggggatc gagtcgggcc ttgaacgcca
     1261 cggcgtccac atagcaagcc ggagcgccgt ccggatcgcc cgggtcgttg cctgtatcga
     1321 ggagatgaag cccgatctcc tcctcctcct cggcgacgtc aagcacaacg tcccggtgac
     1381 gagccggcag gagtaccggg agctccccgg cacccttgcg accctccggg agagggtgcc
     1441 gctcgccgtc gtcccgggaa accacgacgg gggtatcggg cggttcctcg aacccggcga
     1501 actcctgccc cccggcggca cggtcatcga cggcgtcggc tacctccacg gccatacccg
     1561 ccccgacccc gaactcttcg gccgcctcat catcctcggc caccaccacc cggtcgtctc
     1621 tctcgtggat gccgtcgggt gcgctgcacg cgcccggccg gcctacctct actcggggct
     1681 tgatgcgggg tgtgaagtgg agacgccgcc cggcggcaac cgcacccggc tcctctttat
     1741 accggccttc aacgagtttg cggggggtat cgacgtcggg cggctctcgg agagcgggct
     1801 tggccccctc tcccggtgta tagagagaga aaccgcggag gtgttcttag cagatggtac
     1861 gtacgtcggc tccccggcca cgctctcccc cgccggcggc gggtgaactc ctcagccccg
     1921 cggtccggga ggtcgtccgg aagcgcgggt ttaccgctct ttccgatgca caggaacagg
     1981 ccatcccgct cctgctcgag ggcaaaaacc tggtcctggt cgccccgacc ggcacgggga
     2041 agaccgagag cgcgatgctt ccggtcttcg accggctcct tgcgaccgcc ggcccggggt
     2101 tcaaggcggt ctacgtcacc cctctccggt cgttgaaccg cgatatactt gcgcggatgg
     2161 agtggtggtg ccgggaactc tcgctctcgg ccggtgtccg gcacggggat ac
//
LOCUS       DAOSII010000253         2203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_42779_length_2203_cov_39.847300, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000253 DAOSII010000000
VERSION     DAOSII010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42779_length_2203_cov_39.847300"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="PLV90_10990"
     CDS             complement(<1..1028)
                     /locus_tag="PLV90_10990"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOF97682.1"
                     /translation="MTTVGVLTGGGDCPGLNAVIRAVVRTGARHGFDTIGIRDGWRGL
                     IVGDVEPLTDYSVTGILPKGGTILGTSRTNPFKDEENVQRLLDNIRRFGIDALVAIGG
                     EDTLGVANRLSKMGIPVVGVPKTIDNDVAATDYTFGFDTAVSIVTEAIDRLHTTAESH
                     HRIMVVEVMGRHAGWIATVAGIAGGADQILIPEVPFDIDEVVGHLRARYERGKKFSIV
                     VVAEGARPKGQEEAITQSVWLDEFGHVTLGGIGSLLRGEIEDRLGMEARVTVLGHVQR
                     GGSPTAHDRMLATRFGVAAADLIRDKDFGKMVALRGNEIVAVPLEEAVAELKTVDMDL
                     YNIASIFYG"
     gene            complement(1033..1953)
                     /locus_tag="PLV90_10995"
     CDS             complement(1033..1953)
                     /locus_tag="PLV90_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HOF97683.1"
                     /translation="MPPARDIKVPLDVPAGEEERYRENYRTITHGTGRLMLFAGDQKI
                     EHLNDDFYGEGIHPEDADPEHLFRIASRARIGVFATQLSMIARYGGDYRDVPYLVKLN
                     SKSNLVKTSQRDPISSELHHVRQAVDLRDRTGLSIVGVGYTVYVGSEYEAMMLYQAAQ
                     IVNHAHANGLLTVFWMYPRGKAVKDERDAHVVAGAAGVAAALGSDFAKVNPPKVDGKP
                     DAALLREAVAAAGRTGVVCSGGSQISVPEFLQQLHDQIHIGGAVGNATGRNIHQRSLD
                     EAVRFCNAISAITLDDASVDEAMKIYRGGK"
BASE COUNT          365 a          773 c          626 g          439 t
ORIGIN      
        1 ccgtagaata tgcttgctat gttgtagagg tccatatcca cggtcttgag ttctgccacc
       61 gcctcctcga gcgggacagc gactatctcg ttgccccgga gcgcgaccat cttcccgaag
      121 tccttatccc ggatgagatc cgctgccgcc acgccgaacc tggtcgcgag catccggtcg
      181 tgcgccgtcg gcgacccgcc ccgctggacg tggccgagca ccgtcacccg cgcctccatg
      241 ccgagccggt cctcgatctc gccgcgcagg agcgaaccga tgccgccgag cgtcacgtgc
      301 ccgaactcat ccagccagac cgactgcgtg atcgcctcct cctgcccctt cggccgggct
      361 ccctcggcga ccacgacgat gctgaacttc ttcccccgct cgtagcgcgc ccggaggtgc
      421 ccgacgacct cgtcgatgtc gaacgggacc tccggtatga ggatctggtc ggccccgccc
      481 gcgatcccgg ccacggtcgc aatccacccg gcatgccggc ccatcacctc caccaccatg
      541 atccggtggt gcgactccgc cgtcgtgtgg aggcgatcga tcgcctcggt gacgatcgag
      601 acggcagtat cgaacccgaa ggtgtagtcg gtcgcagcga cgtcgttatc gatggtctta
      661 gggacaccca ccaccgggat acccatcttc gagagcctgt ttgcgacacc gagggtatct
      721 tcgccgccga tcgcgaccag cgcatcgata ccgaaccttc tgatgttgtc cagcaaccgt
      781 tgcacgttct cctcgtcctt gaaggggttc gtccgcgacg tcccgaggat cgttccccct
      841 ttcgggagga tccccgtcac cgagtagtcg gtgagcggtt cgacgtcccc gacgatcaga
      901 ccgcgccatc cgtcccgtat cccgatcgtg tcaaaaccat gtcttgctcc ggtccgcacg
      961 accgcccgga tcaccgcgtt caggcccgga cagtcccctc cgcccgtcag tacgcctacc
     1021 gtcgtcatct catcactttc cccctctgta gatcttcatc gcctcatcca cactcgcatc
     1081 gtcgagggtg atcgccgata tggcgttgca gaaccggacc gcctcgtcaa gcgaccgctg
     1141 gtggatgttc ctcccggtcg cgttcccgac cgcaccgccg atgtggatct ggtcgtggag
     1201 ctgttgcagg aactccggca cgctgatctg cgatccgccc gaacagacga cgcccgtcct
     1261 gccggccgcc gccaccgcct cccggaggag tgcggcatcg ggcttcccgt cgaccttcgg
     1321 cgggttcacc tttgcaaaat cgctcccgag agccgccgcg acccccgccg cccccgcgac
     1381 aacatgcgcg tcgcgctcgt ccttgaccgc tttgccgcgg ggatacatcc agaagacggt
     1441 gaggagcccg tttgcatgcg cgtggttgac gatctgggca gcctggtaga gcatcatcgc
     1501 ctcgtactcg cttcccacat agaccgtgta gccgacaccg acgattgaaa gccccgtccg
     1561 gtcccggagg tcgacggcct gccggacgtg gtggagctcc gagctgatcg ggtcccgctg
     1621 agaagtcttg accagattgc tcttcgagtt gagtttgacg aggtagggca cgtcccggta
     1681 atccccgcca taccgtgcta tcatgctcag ttgcgtcgca aagaccccga tcctcgcccg
     1741 gcttgcgatc cggaagaggt gttcggggtc ggcgtcctca ggatggatgc cttcgccata
     1801 gaaatcgtcg ttgaggtgtt cgatcttctg atccccggca aagagcatca atctcccggt
     1861 gccgtgcgtg atcgtccggt agttctcccg gtacctctct tcctccccgg cgggcacatc
     1921 gagaggaacc ttgatgtcgc gtgcaggtgg catgcttccc cctcccggcc cccacatact
     1981 taaaagttgc tttgatcgcg ggagatgggc cgaaagggcc ggatctccag ggcaccaggg
     2041 taaggttaac cgctctttcg gaaggcgaaa tctcccccgg agagcgtact gtcccgccgg
     2101 gccctatggt cgagtagcat ttcagaacgt ttattttgct ctccgataga actattcata
     2161 gaatgcccgg cgctgtcctt ccggttgaac acagcgccgt aca
//
LOCUS       DAOSII010000254         2180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_43394_length_2180_cov_38.490824, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000254 DAOSII010000000
VERSION     DAOSII010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43394_length_2180_cov_38.490824"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="PLV90_11000"
     CDS             complement(<1..1193)
                     /locus_tag="PLV90_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOF97684.1"
                     /translation="MSDTIAIIGGGPAGLFCALQAAGRGRRVILLEKKPAPARKLLIT
                     GSGQCNITHDGEVTGFLSHYGDHGAFLRPALRNFTNRDLIAFFADRGLPMQAEPGGKV
                     FPETRRAADVLAVLLSECSARGVAIRCGEPVVAVERDGDGFLVRTEKESVRAGVLVIA
                     TGGASYPATGSTGDGYAFARALGQPVTEVAPALTPVSIEGYPFADLAGISFENAAFAI
                     HRGGRVVRRHTGDILLTHAGLSGPGILDASRFILPGDVLRVTFLCGANAEAVRKRVAG
                     ALREGGARQAKTILCDLPLPERFARRLLDIAGVPAGATCAHLPRKARDALAASVAEFP
                     FTVARLGGFHEAMATRGGIALDGIDPKTMVSRVVRNLYCIGEVLDIDGDTGGYNLQAA
                     FSTAAL"
     gene            1308..>2180
                     /locus_tag="PLV90_11005"
     CDS             1308..>2180
                     /locus_tag="PLV90_11005"
                     /inference="COORDINATES: protein motif:HMM:NF021607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HOF97685.1"
                     /translation="MNDTWILLMGGVLLVLLITPGSAASEGAPGTGGFAGRQAIAIDH
                     PSGTEGLEVLFNATYLPGMRPDFADIRFLDGNETAIPHWTATVMEGSHAHVWLALPAN
                     ATTITMLYGNPAARDAGDPDAVFLFYEDYERGDLSRWSFCGSNAAVRSDVVRSGGYAG
                     DINVSEPDLRELANNRICLANISAGPIIVEGDFLVSKFGKWCGSGYIQAWSGPDRLYA
                     LHLRNGSVQYYDGAHRNFSADARAEEETWYHIKVALDTPNATGHAWMDGRYLGKMTLR
                     FADGSPVPEETIFTD"
BASE COUNT          380 a          743 c          714 g          343 t
ORIGIN      
        1 agggccgcgg tggagaatgc cgcctgcagg ttgtagcccc cggtgtcgcc gtcgatatcg
       61 agcacctcgc cgatgcagta gaggttccgc acgacccgcg agaccatcgt ctttgggtca
      121 atcccgtcga gggcgatgcc gccccgcgtc gccatcgcct cgtgaaaccc cccgaggcgt
      181 gccacggtga atggaaactc cgcgacggat gcggcaagcg catcccgggc cttccggggg
      241 aggtgggcgc atgtcgctcc cgccggaacc ccggcgatat cgagcagccg ccgggcgaac
      301 cgctccggga gggggaggtc gcagaggatg gtctttgcct gccgggcccc gccctcccgg
      361 agcgcgccgg cgaccctttt tcgcaccgct tccgcgtttg cgccgcagag gaacgtgacc
      421 cggagcacgt cgcccgggag gatgaaacgg gaagcgtcaa gtatgcccgg gccggagagg
      481 ccggcgtggg tcagcaggat gtcgcccgtg tgccgccgga ccacccttcc gccccggtgt
      541 atggcaaagg cggcattctc aaacgatatc ccggcaaggt cggcgaacgg gtagccctcg
      601 atggagaccg gggtgagcgc cggggcaacc tccgtgacgg gttgcccgag ggcccgggca
      661 aaggcgtaac cgtccccggt agaaccggtg gcgggatagg aggcgccccc ggtggctatg
      721 acgaggacgc cggcccggac actctccttc tccgtccgga cgaggaaccc atccccgtcc
      781 cgctccacgg cgacgaccgg ctcgccgcac cggatagcaa ccccgcgggc ggagcactcc
      841 gaaaggagga cggcaagcac atcggcggct cgccgggtct ccgggaagac ctttcccccg
      901 ggttcggcct gcataggaag gcccctgtcg gcaaagaagg cgatgagatc ccggttcgtg
      961 aagtttcgga gcgcggggcg gaggaacgcc ccatgatcgc cgtaatggga gagaaaaccg
     1021 gtgacctccc cgtcatgggt gatgttgcac tgtcctgagc cggtgatcag gagttttcgc
     1081 gccggtgcag gcttcttctc aaggaggatc accctccgcc ctctccccgc tgcctgcagg
     1141 gcgcagaaga gccccgcagg ccctcccccg atgatcgcga tggtgtcgct cacggttata
     1201 ccctgtaccg tgcttccccg gggaacatca gcgttggggt gcgccggccg atacacatat
     1261 gggggtgggg cccgtaatgg gccggagccc tgaaacgagt gatccgaatg aatgatacct
     1321 ggatacttct catgggagga gtcctgctcg tcctcctcat cacacccgga tctgctgcct
     1381 ctgaaggtgc acccggcacc ggaggttttg ccggccggca ggcgatcgcg atcgaccacc
     1441 cctccgggac ggaagggctt gaggtcctct tcaacgccac gtacctcccc gggatgcggc
     1501 cggacttcgc cgacatacgg ttcctggacg ggaacgagac cgccatcccc cactggacgg
     1561 caaccgtcat ggagggctcc catgcccacg tctggcttgc ccttcccgcg aacgcgacca
     1621 cgatcaccat gctctacggc aacccggcgg cccgggatgc aggcgacccc gacgccgtct
     1681 tcctcttcta cgaggactac gagcgcggcg acctctcccg atggtcgttc tgcggctcaa
     1741 acgctgccgt ccggtcggat gtcgtccgga gcggtggata cgcaggcgat atcaacgtct
     1801 ccgagcccga cctccgggaa ctcgcaaaca accggatctg ccttgcaaat atatccgccg
     1861 gccccatcat cgtcgaaggg gacttcctgg tcagcaagtt cgggaaatgg tgcggatcgg
     1921 gctacatcca ggcctggagc gggcccgacc ggctctatgc cctccatctc aggaacggct
     1981 ccgtccagta ctacgacggc gcccaccgga acttctccgc cgatgcccgg gcggaggagg
     2041 agacctggta ccacatcaag gtcgccctcg atacgccgaa cgccaccggg cacgcgtgga
     2101 tggacggccg gtacctgggg aagatgaccc tgcggttcgc cgacggttca cccgtcccgg
     2161 aggagacgat ctttaccgac
//
LOCUS       DAOSII010000255         2175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_43522_length_2175_cov_41.713679, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000255 DAOSII010000000
VERSION     DAOSII010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43522_length_2175_cov_41.713679"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..985)
                     /gene="mch"
                     /locus_tag="PLV90_11010"
     CDS             complement(38..985)
                     /gene="mch"
                     /locus_tag="PLV90_11010"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HOF97686.1"
                     /translation="MLSVNELALDIFEELFEYAEEFHAVPHEFDNGARIVDCGVSTTG
                     GYLAGRRFTEICMGGLGEVDITMGRIRDVPIPFIEVSTDFPAIACLGAQKAGWTVNVN
                     KYFAMGSGPARALSLKPKHTYEVIDYEDEFDYAVIALESDHLPNAGVMEKIADACDVD
                     VANTCAVVAPTASIVGSVQVAGRCVETAVYKLNELGFDTRKITAGIGHAPIAPVKKDG
                     TRAMGSTNDATIYHGSIMLTMNAPEIKDYLDRIPSNKSRGYGKPFYEIFKEAKFDFYQ
                     IDTSLFSPAEVVINELSEGAVYHVGAVNPDVTLKSFGFI"
     gene            complement(1035..>2175)
                     /locus_tag="PLV90_11015"
     CDS             complement(1035..>2175)
                     /locus_tag="PLV90_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ORC1-type DNA replication protein"
                     /protein_id="HOF97687.1"
                     /translation="SLLMWDETLFRDPEVFEIDYVPEQFNHRDTQIRELAFQVRPGLR
                     GGRPLNTICRGLPGTGKTTSVRKVFAEIEEVTQKLVPIYINCQIDNTKFAVFSQIYRR
                     IFGHLPPASGVSFKQLLDAIARALQREEQVLLVGLDDANYLLYENEINHVLYSLLRSH
                     EAYPGVRIGVVAIVSDMSVSLQTEVDARVASVFRPTEIYFPPYSEEEIRGILGERVVQ
                     GLYPNVLRSDLLDLVVEQTTKSGDLRVGIDLLKRATLSAEKAARRSIEQEDICKAYKV
                     SRYLHLAYSLRALRAEEREVLGRIAEMAIRDDRETNAGEVYHFVREHVGISYTRYYKM
                     IQKFDAMRLLNLHYRQGRGRTRLISLRYDPDRVLEHLRREQTGSI"
BASE COUNT          418 a          645 c          661 g          451 t
ORIGIN      
        1 cgcccgggtc ggtcaggaaa agggaggaga gatgagttca gatgaacccg aaagatttga
       61 gcgtcacgtc ggggttgacg gcccccacgt gatagacggc cccctcggag agctcgttga
      121 tgacgacctc ggcgggggag aagagcgatg tatcgatctg gtagaagtcg aacttggctt
      181 ccttgaagat ctcatagaac ggttttccgt atccccggga cttgttgctc gggatcctgt
      241 caagataatc cttgatctcc ggggcgttca tcgtcagcat gatgctgccg tggtagatcg
      301 tggcgtcgtt ggtgctgccc atcgccctcg tgccgtcctt cttgacgggg gcgatcgggg
      361 catggccgat accggcggtg atcttccttg tatcaaaacc gagctcgttc agtttgtaga
      421 ctgccgtctc gacgcagcgg ccggcaacct ggaccgagcc gacgatcgat gccgtggggg
      481 cgacaaccgc acaggtgttt gccacgtcca cgtcgcaggc gtcggcgatc ttctccatca
      541 cgccggcgtt cgggagatgg tcgctctcga gggcgatgac cgcataatcg aactcgtcct
      601 cgtagtcgat gacttcgtaa gtgtgcttcg gctttaagga gagcgctcgt gccggaccgc
      661 tgcccatggc gaagtacttg ttgacgttga ccgtccagcc tgctttctgg gcgccgaggc
      721 atgcgatggc cgggaagtcg gtgctgacct cgatgaacgg gatagggaca tccctgatcc
      781 ggcccatcgt tatgtcgacc tcgccgagtc cgcccataca gatctcggtg aaccgtcttc
      841 ctgccaggta cccgccggta gtgctgacgc cgcagtctac gatgcgtgcc ccgttgtcaa
      901 actcgtgcgg gacggcatgg aactcttcgg cgtattcgaa gagttcctca aaaatatcca
      961 gtgccagttc gtttacgctg agcatgtgga aaacctcatc agaatacaaa cggtggcaga
     1021 acagataagg attatcaaat gctacctgtc tgctcccgcc gcaggtgctc aagcacccta
     1081 tccggatcgt aacggaggct gatcagccgc gtccgccccc ttccctgccg gtaatggagg
     1141 tttaagagcc gcatcgcatc gaacttctgg atcatcttgt agtaccgggt atagctgatc
     1201 cccacatgct ccctgacgaa gtgatagacc tctccggcgt tcgtctcccg gtcgtcacga
     1261 atcgccatct cggcgatcct gccgagcacc tcccgctcct cggccctgag cgcccggaga
     1321 gagtacgcaa ggtgcaggta ccgggagacc ttgtaagcct tgcagatatc ctcctgctcg
     1381 atggaccggc gggccgcttt ctcggcgctc agggtcgccc gcttcagaag gtcgatgccg
     1441 acgcgaaggt cgccgctctt cgtcgtttgc tctaccacga gatccagcag atcggaccgg
     1501 aggacgttcg ggtagagccc ctgcacgacc cgctccccaa ggataccccg gatctcctcc
     1561 tccgaatagg gcgggaagta tatctcggtg ggccggaaga ccgacgccac gcgggcatcc
     1621 acttcggtct gcaggctgac ggacatatcg ctgacgatgg cgacgacacc gatccggacg
     1681 ccgggatatg cctcgtgaga acggagcagg gagtaaagga cgtggttgat ctcgttctca
     1741 tagagaaggt agtttgcgtc gtccagtccc accaggagca cctgctcctc gcgctggaga
     1801 gcccgggcta tggcatccag gagctgtttg aacgatacgc ctgatgccgg cggcaggtgc
     1861 ccgaagatcc gccggtagat ctgcgaaaag acagcgaatt tggtgttgtc gatctggcag
     1921 ttgatataga tgggaaccag tttctgcgtg acctcctcga tctcggcaaa caccttcctg
     1981 acgctcgtcg tcttccctgt cccgggaaga ccccggcaga tggtgttgag gggccgccct
     2041 ccccggaggc cgggccggac ctgaaaggcg agctcccgga tctgggtgtc acggtgattg
     2101 aactgctcgg gaacgtagtc gatctcgaag acctcagggt cccgaaagag cgtctcatcc
     2161 cacatgagca ggctc
//
LOCUS       DAOSII010000256         2103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_45603_length_2103_cov_43.737305, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000256 DAOSII010000000
VERSION     DAOSII010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45603_length_2103_cov_43.737305"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..414)
                     /locus_tag="PLV90_11020"
     CDS             complement(16..414)
                     /locus_tag="PLV90_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97688.1"
                     /translation="MHIASGTKLFGFGILTWLLPFLVSVPFYSPEGEPLYDIFLIKSV
                     LLVFSAALGTALILVYFRGVRARYIREGALLGGVWLAINWALDVAVLLPLSGMDIGAY
                     MGEIGLRYLMIPIIAVGIGYAAEQAVRGRG"
     gene            893..>2103
                     /locus_tag="PLV90_11025"
     CDS             893..>2103
                     /locus_tag="PLV90_11025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF97689.1"
                     /translation="MFAFVLMSVYQYLKDYLYPEISLADSHLHTVVFTTLLATAAAYF
                     VLRKQQALLAMLVAEANERRETGEALRTSEEKFRSIVETANEGILQIDSAYTTTYVNR
                     TMAGMLGYPTGEMLGRPLADFVVAGDLDVYWSRMAAREQGLDGRYECRLRHSDGSIRC
                     FLVSATARKDGDGRFAGSFAMFTDITERKRTEEALQRHTEDLARLHQQLAAANREANL
                     YLDILTHDIKNTENVANLYSDLLIDTLDGDARIHAEKLQRSIRKSIEILGNVSKIRRI
                     HQPSGGRKQMDLDAVVRGEIAQFPEATIRYAATGCRVLADDLLSEVFANLIGNAVKFG
                     GPEVEIAIRVDDEGEFFRVSVEDTGPGVPDDQKEEIFHRYEKRRRGIGEGLGLFLVQV
                     LIERYGGRIWV"
BASE COUNT          471 a          639 c          641 g          352 t
ORIGIN      
        1 gcaggtgcgg gaaggttatc cccttccccg gacggcctgt tccgccgcat acccgatacc
       61 gaccgcgatg atcgggatca tgaggtaacg cagcccgatc tctcccatgt aggcgccgat
      121 atccatcccc gaaaggggca ggaggacggc gacatcgagc gcccagttga tcgcgagcca
      181 tacgccgccg agcagggcgc cttcccggat ataccgggca cgcacacccc tgaaatagac
      241 caggatgaga gcggtcccga gcgcggctga aaagacgagc aggaccgact tgatcaggaa
      301 gatgtcgtag agcggttcgc cctccggcga gtagaagggg acggatacga ggaaaggaag
      361 gagccaggtg aggatgccga agccgaaaag cttcgtgcct gacgctatgt gcatggatga
      421 ggatgatact ccggcgcgaa tataccaatc ggacatacgc cgggagaaag cagggcaata
      481 gccctccctg ccggcgccgt cacggaacgg atgcaccgga aagcagcgta gcgcgggttg
      541 ccggcgggtt cttccggaga atccccgcgg ttcgctaaag cttctgcatc attaacaaag
      601 gatcgtgatt acggtcggcc cgccaccgac ggaaggttta tgaaatctca ggggagaatg
      661 caatcaggca ccgcatatat gctgtcactc cggtccagcc ccggaacgcg aaacctggcc
      721 gatacaggca gtattcaggc cgatggatca gccagggccg aacattccac gatatctccg
      781 tagatcagcg atctcccgac atgcagaaca tcctgtaccg cttctccgtg gagcgcgcat
      841 gaataacgag ttacaacccc ccaacctcta tctcactctc gcaagggtgg gagtgttcgc
      901 attcgtcctg atgagcgtat accagtatct caaggattac ctctaccccg agatctcgct
      961 cgcagactcg cacctccata ccgtcgtctt caccaccctg ctcgccacag ctgctgccta
     1021 tttcgtcctc cgtaaacagc aggcccttct tgcgatgctg gttgccgaag cgaacgagag
     1081 gagggagacg ggtgaagccc tgcgaacgag cgaggagaag ttccgctcga tcgtagagac
     1141 ggcaaacgag gggatcctgc agatcgacag cgcctacacg accacctacg tcaaccgtac
     1201 gatggccggc atgctcggct acccgaccgg ggagatgctc ggccggcctc tggccgattt
     1261 cgtggttgca ggggacctcg acgtatactg gagccgcatg gcagcaagag agcagggcct
     1321 tgacggtaga tacgagtgcc gcctcaggca cagtgacgga agcatccggt gcttcctcgt
     1381 ctccgccact gcacggaaag atggggacgg aaggtttgcc gggtcgtttg ccatgttcac
     1441 cgatatcacc gagcgcaaac ggaccgagga agcgctccag cgtcacaccg aagatcttgc
     1501 ccggctccac cagcagctcg ccgcagcgaa ccgcgaagca aacctctacc tggacatact
     1561 tacccacgac atcaagaaca ccgagaacgt cgcgaacctc tacagcgacc ttctgatcga
     1621 caccctggac ggggatgcgc ggatacacgc ggagaagttg cagcggagta tcaggaagag
     1681 catcgagatc ctcgggaacg tctccaaaat ccggcggatc caccaaccgt ccggggggcg
     1741 caagcagatg gatctcgatg cggtcgtccg cggtgagatc gcgcagttcc ccgaggccac
     1801 catccgctac gctgcgaccg gttgccgggt gctggccgac gacctcctct ccgaggtctt
     1861 tgcgaacctc atcggcaacg ccgtcaagtt cggcgggcca gaggtcgaga tcgctatccg
     1921 ggttgacgac gaaggcgagt tcttccgggt ctcggtcgag gataccggcc cgggggttcc
     1981 cgacgaccag aaagaggaga tctttcaccg ctacgagaag aggaggaggg gtatcggcga
     2041 ggggctcggc ctcttcctcg tgcaggtcct catcgagcgc tacggcggca ggatctgggt
     2101 gga
//
LOCUS       DAOSII010000257         2086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_46130_length_2086_cov_45.893648, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000257 DAOSII010000000
VERSION     DAOSII010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46130_length_2086_cov_45.893648"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(272..463)
                     /locus_tag="PLV90_11030"
     CDS             complement(272..463)
                     /locus_tag="PLV90_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97690.1"
                     /translation="MVVLRLLAMDDVSFCSWEGNGSDAYGDIWVIIPEKNLFPEFLKH
                     FCRASGRDLVSLPQSENFF"
     gene            617..1633
                     /locus_tag="PLV90_11035"
     CDS             617..1633
                     /locus_tag="PLV90_11035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF97691.1"
                     /translation="MTANRVDDRDTWDSFIDESPSGLLFHRWDYLHLTAKHTGSTLHP
                     YAICKGDELICLFPLFYRRMHGITAVFSPPPLTVIPHLGCVMSENFERLKQSKKESVL
                     HLVAEDIRGTLADLSPNYLSIAFVPEFNDIRHYLWDHCDARIRYTYTINLERPLSEIQ
                     DGLHYKLRNQLKKEERAGLQLEKTNDISTLHRLLADRYRDPSLDIPPIKREYLKDLVR
                     AYPERIGVYHLYDAGGDLAGVVATQEYKRFLLWMGTPRLKSAHAGNEYLQWLLIRRAK
                     AEGYRTFENMGANNPDLAFFKSRFNPDLTTYFEIEKMDGLGMVSRWAYLTLAKRLMMA
                     AGRV"
     gene            1836..>2086
                     /locus_tag="PLV90_11040"
     CDS             1836..>2086
                     /locus_tag="PLV90_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF97692.1"
                     /translation="MPVEVVSDKEAWDTFVDAAPSGLLFHRWDFLKVTGRWTGYTVLP
                     YGIYRGEELIAVCPLFHKRICGLSIVLSPPPLQAVVPYLG"
BASE COUNT          451 a          630 c          630 g          375 t
ORIGIN      
        1 cagccgcgag agcgcctctg cctggtaggc cagaagttcc tcccggctcc accagtggga
       61 ccgctggagg agggcatagg tcctgatgta ggcagagtac gcggggagga tatggagcgc
      121 tctcattgtc atggtgtctt ccagctggag ggctctcaat ctccgatata ctgcccgcac
      181 acccatcccg gcactgctca tgcgatcctc tgcattaaac gttgtgctcc gggcgagacg
      241 gcacggtgcc gacggtccgg ggtgtctgca gctaaaagaa gttctcggat tgcgggaggg
      301 agacgagatc gcggcccgaa gcccggcaga aatgtttcag gaactcggga aagagattct
      361 tctccggaat gataacccat atgtcgccgt acgcatcgga tccgttgccc tcccacgagc
      421 agaacgagac gtcgtccatg gcaagcaggc ggagcacgac catgccccta cctcacgtgt
      481 tgctgtatat aagcattgtg ccggggaacc cggcgccatg cccggacgac catccggaga
      541 aagatatatg attccccggg gggattagag gtcgccggac gacgggcagc gcgaccgaac
      601 gaaggtgacg aaaggaatga cggcaaatag ggtcgacgac agggatacat gggactcctt
      661 catcgacgag agcccatccg gactcctctt tcacaggtgg gactacctgc acctgacggc
      721 aaagcatacc gggagcacgc tccatccgta cgcaatctgc aagggagacg agctcatctg
      781 cctctttccg ctcttttaca ggcggatgca cggcatcacc gccgtcttct cccctccgcc
      841 gctcaccgtg atacctcacc tcgggtgcgt catgagcgag aacttcgaga ggctcaaaca
      901 gagcaagaag gagtcggtgc tgcacctggt cgccgaagat atccggggaa cgctcgcgga
      961 tctctcaccg aactacctct cgatagcctt tgtccccgaa ttcaacgata tacgccacta
     1021 cctctgggac cactgcgacg cgaggatccg gtatacttac accatcaacc tggagcggcc
     1081 gctctcggag atccaggatg gccttcacta taagctccga aaccagctca aaaaggagga
     1141 gagagccggc cttcaactcg agaagacgaa cgacatatcg acgctccacc ggctccttgc
     1201 cgaccggtac cgggatccgt cgcttgatat cccgcccatc aagcgggagt acctcaaaga
     1261 cctcgtgcgt gcgtatcccg agaggatcgg ggtgtaccat ctctacgatg cgggagggga
     1321 tctcgccggc gtggtggcga cccaggagta caaacggttt ctgctctgga tggggacgcc
     1381 acggttaaag agcgctcatg cggggaacga gtacctccag tggctcctga tccggcgggc
     1441 aaaggccgaa ggctaccgga cgttcgagaa catgggcgca aacaacccgg acctggcctt
     1501 cttcaagtca aggttcaacc ccgacctcac gacctacttc gagatcgaga agatggatgg
     1561 tctcgggatg gtctcgcggt gggcgtacct caccctcgcc aagcgattga tgatggcggc
     1621 aggcagggtt taggatcggg gcagggttcc ccgcacgaga acggcggaac agatatccga
     1681 taccggcggc gaccgccaca tttatcggga tgaccgcagt tccgggaggg gaagcaactg
     1741 cagccctacc tcggcggcga ccgccacatt tatcgggatg actgcaggaa tagggagcct
     1801 ccgggggtac ccggaaggag ggggagaaga acaacgtgcc ggtcgaggta gtctcggata
     1861 aagaggcatg ggatacgttc gtcgatgccg caccgtccgg ccttctcttc caccgatggg
     1921 acttcctgaa ggtcaccggg cggtggaccg ggtatacggt cctcccctac ggcatttacc
     1981 ggggcgaaga actcatagcc gtctgcccgc tcttccataa acggatctgc ggcctctcca
     2041 tcgtgctctc gcctccgccc ctgcaggcgg tcgtgcccta cctcgg
//
LOCUS       DAOSII010000258         2085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_46167_length_2085_cov_46.086700, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000258 DAOSII010000000
VERSION     DAOSII010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46167_length_2085_cov_46.086700"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..1170)
                     /locus_tag="PLV90_11045"
     CDS             complement(82..1170)
                     /locus_tag="PLV90_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97693.1"
                     /translation="MKRIAWLTLIAAMLLVSAASAAYVSISAPKEVYVGDRLVVAGTS
                     VAGEFAQPSLKPGFSTDVILYYAKGTKSEVARKTIVVQENGLFSATFDTAGLAAGTYS
                     IEIVDPTQTTFGGSSKVQQMVELIDRSKDVVVDSPLTQEFDGTLDLRGTVSGIGDAGV
                     QVRVEHDAAVIYGPKYIRTDANGAFSEEVPIPDGGTYGVTFSDTKGFISTVTFVISGK
                     PAPTSTPAMISANAPATRSAPAYFEVDTKSGTVTITTSVGIDWVIEYIDEDGGSHKVN
                     DKGQLEPETAEFVARGGTVYVKVYPMSYSDRGTVQVSAANADSIRVSQAAPGLFGDAV
                     PTTAQATPLPAFLALLALIVVVVLARRG"
     gene            1199..>2085
                     /locus_tag="PLV90_11050"
     CDS             1199..>2085
                     /locus_tag="PLV90_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosine biosynthesis radical SAM protein
                     RaSEA"
                     /protein_id="HOF97694.1"
                     /translation="MVSKSIEKPLASWRGKDLYEGQVLDTLTVIFRSGGCSWNRCRMC
                     GYRHERYPDLPQDDLADRLIRQVRWVKENFCDEDYQVLKIFTSGSFFDPDEVPPAVRR
                     AVAEAFRGKAVIAETRPEYVESDALREFIEGIDTGVWSRPLSVAVGLETTNDAIREKS
                     IDKGFTYADFLRAAGIAHAAGAGVKAYLLMKPPFLTEREACDDMVRSIRDVASVADSI
                     SMNLCTVQSRTEVERLWREHAYRPPYLWSVLDVLIKSPVHILCDPVGGGQTRGPHNCG
                     ACDGPIVKGIGDYSLTGDV"
BASE COUNT          388 a          644 c          650 g          403 t
ORIGIN      
        1 ggctttcaag gccgccgcct tcccggacta gactatcccg gcccgcctat acctattggc
       61 cgccggacga aaaaaggttg ttcatccccg gcgcgcgagg acgaccacaa caatgagtgc
      121 aaggagcgcg aggaacgccg ggagcggcgt ggcctgggcg gtggtgggca ccgcatcccc
      181 gaagagtccg ggcgcagcct ggctcaccct gatcgagtcg gcgttcgcgg ccgagacctg
      241 caccgttcca cgatcgctgt aactcatggg gtagaccttg acgtagacgg tgccgccccg
      301 tgcgacaaac tccgcggtct caggttcgag ctggcctttg tcgttgactt tgtgagatcc
      361 cccatcctca tcgatgtact cgatcaccca gtcgatcccg accgatgtcg tgatcgtcac
      421 cgtaccgctc ttcgtgtcga cctcgaagta ggccggggcg gagcgcgtgg cgggggcgtt
      481 cgcggatatc atcgccggcg tcgacgtggg agcgggctta cccgagatca cgaacgtcac
      541 ggtgctgatg aaacctttgg tgtcggagaa tgtgaccccg taggtgccgc cgtcagggat
      601 gggcacttct tcggagaatg ctccgtttgc gtcggtgcga atgtacttcg gcccgtatat
      661 gacggcggca tcatgctcga cccggacctg aacgcctgca tcgccgatcc cgctgaccgt
      721 gccccggagg tcgagcgtgc cgtcgaactc ctgggtgagc ggggaatcta ctacgacatc
      781 cttggagcgg tcgataagct cgaccatctg ctggaccttc gaactgcccc cgaatgtggt
      841 ctgcgtggga tcgacgatct cgatcgagta ggttcccgca gcaagaccgg cagtatcgaa
      901 cgtcgctgag aacaaaccat tctcctgaac gacgatggtc tttcgggcta cctcgctctt
      961 cgtgcccttg gcgtagtaca ggatgacgtc cgtgctgaac cccggtttga gcgaaggctg
     1021 tgcgaattct ccggcaaccg atgtgccggc gacaacgagc cggtcaccga cgtatacctc
     1081 ttttggggca ctgattgaaa catacgcggc tgatgccgcg ctgacgagca gcatcgcagc
     1141 gatgagcgtc agccatgcga tccttttcat accagtacat caacgaatat gaggtataat
     1201 ggtatccaaa tcgattgaga agccgctcgc gtcctggcgg gggaaagacc tctacgaagg
     1261 gcaggtcctc gatacgctga cggttatctt caggagcggc gggtgttcct ggaaccggtg
     1321 ccgtatgtgc ggctaccggc acgagcgcta ccctgatctg ccgcaggacg accttgccga
     1381 ccggttgatc cgccaggttc gctgggtgaa agagaacttc tgcgacgagg actaccaggt
     1441 gctcaagata ttcacgtcgg gcagtttctt cgatcccgac gaggttcccc ctgccgtccg
     1501 gcgcgccgtt gcggaagcgt tccgtggtaa ggccgtcatc gccgagacaa ggcctgaata
     1561 cgttgaatcg gacgcgctcc gtgagtttat cgagggcatc gataccggcg tctggtcgcg
     1621 gccgctctcc gtcgccgtgg ggcttgagac cacgaacgat gctatccggg agaagagcat
     1681 cgataaaggg ttcacctacg ccgatttcct ccgcgccgca gggatcgcgc acgccgccgg
     1741 cgccggcgtg aaggcttacc tcctgatgaa accgccgttt ttgaccgagc gcgaggcgtg
     1801 cgacgacatg gtgcgctcca tcagggatgt cgcatccgtt gcggacagca tctccatgaa
     1861 cctctgcacc gtccagagca ggaccgaggt cgaacgcctc tggagagagc acgcataccg
     1921 ccctccctac ctctggagcg tcctcgatgt gctgatcaaa tccccggtcc atatcctctg
     1981 cgaccccgtc ggcggcgggc agacccgggg gccgcataac tgcggcgcct gcgacggccc
     2041 gatcgtgaag gggatcggcg actactcgct gaccggcgac gtcga
//
LOCUS       DAOSII010000259         2060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_46910_length_2060_cov_40.213466, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000259 DAOSII010000000
VERSION     DAOSII010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46910_length_2060_cov_40.213466"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..900)
                     /locus_tag="PLV90_11055"
     CDS             complement(127..900)
                     /locus_tag="PLV90_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOF97695.1"
                     /translation="MNHDLKAAVLARCRSMEIPLVGVASTDRWENPPFEPWMPEEFYP
                     QSIFPEARSVIVIGLPVHLPVLRTAPSIYYHELYRTINTLLDQYTYRLASFLNDRGYP
                     SVFIPRDGYGGIDVLLKNPVAFFSHRHAAFLAGLGTFGVNNALITPEYGPRVRFGSIL
                     TAAPLPPDPLREEGLCTRCMECVTACPAGALDGRDYPEGLTDKAACARNSADLARRHI
                     SPCGICIQVCPVGEDRRHYGGEDSRHRRAREHVQKYGGL"
     gene            1180..1446
                     /locus_tag="PLV90_11060"
     CDS             1180..1446
                     /locus_tag="PLV90_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97696.1"
                     /translation="MSEAKGHFERGRWVVDPVPAPEPEKKPAPKTPIVEERTHEAPKP
                     VQKSGSHPPDAHEQIELAARKAGEDLERAINAWAASARQGLQKG"
     gene            complement(1453..>2060)
                     /locus_tag="PLV90_11065"
     CDS             complement(1453..>2060)
                     /locus_tag="PLV90_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HOF97697.1"
                     /translation="CHSMGTCIARYLLEVLDGGTREEGVRQLVGIGPPNNGSAMAELF
                     NDPLRGPEVIRSLAGVFVPPDYDPGDDTIVQEFRPRSRIVAALRAAGTRDDIAYRIVL
                     AVNLTRTPAFFPIFDGRTWEIAPDGGWRATYAGDGIVPHIDSHLPGVRTDVIPADPVN
                     FARHPEQYCHITLPRNPEVVARIMEHLCSPDAGSAGGSPAP"
BASE COUNT          378 a          623 c          721 g          338 t
ORIGIN      
        1 ttgcgctcgg gctcggtgcg gcgatcgcca tcatcgtcgg gttcggcctc cgggaccgcg
       61 cgccggagat catggatcgg cttattggcc gggtaagata gacataaccc tttttccggg
      121 cgggtctcac aacccgccgt acttctgcac gtgttcgcgg gcccggcggt gccgggagtc
      181 ctcgccgcca tagtgccgcc ggtcctctcc caccgggcag acctgtatgc agataccgca
      241 gggagatata tgtcgtcggg cgaggtcggc gctgttcctg gcacaggcag ccttatcggt
      301 gaggccttcg gggtagtccc ggccgtcaag cgcgcccgcg gggcaggcgg tgacgcattc
      361 catgcaccgg gtgcagaggc cttcttcccg caaggggtcg ggcgggaggg gtgcggcggt
      421 gaggattgag ccgaacctga cccggggacc gtattcgggc gtgatgaggg cgttgttcac
      481 accaaacgtc ccgaggccgg cgaggaaggc ggcgtgccgg tgcgagaaga aggcaaccgg
      541 gttcttcagg aggacatcga tgcccccgta gccgtcacgg gggatgaaga ccgaagggta
      601 gccgcgatcg ttaaggaacg acgcaagacg gtaggtgtac tggtcgagca gtgtgttgat
      661 cgtcctgtat agctcgtggt agtatatcga gggggcggtc ctgagcaccg ggaggtggac
      721 cggaaggccg atgacgatca ccgatcgggc ctcggggaag atcgactgcg ggtagaactc
      781 ttccggcatc cagggttcaa acggcgggtt ctcccaccga tccgtgctcg cgaccccgac
      841 gagcgggatc tccatactcc ggcaccgcgc gaggactgcc gccttcaggt cgtggttcat
      901 aactactggt accctgcccc ggcgaaaaaa gattctccgg tcaggtgccg gccgggaacg
      961 gtcccactgc cagaggatca ggcaggtgaa ccgcgatcac ccggccggct ctcgtgcgga
     1021 ggtggaaggc gccccccgag tatcggaaga gtcgcgcgac gataccggag ttatccgcca
     1081 gtttggagtg ttcggtcctc atttcaacca gatacatctt tctcgaacga tgcggtgcaa
     1141 aaagccaggt ttttattccc ggcggatcca tcaccgcata tgagtgaggc aaaaggccac
     1201 tttgagcggg gacgatgggt cgtcgacccg gtacccgctc ctgaaccgga gaaaaagccg
     1261 gcaccaaaaa caccgatcgt tgaagaacgc acgcacgagg ccccaaaacc agtccagaaa
     1321 tcaggcagcc acccgcccga tgcgcacgag cagatagaac tggcggcacg gaaggccggg
     1381 gaggacctcg aacgggcgat caacgcctgg gcggcgtctg cgcggcaggg tttgcagaaa
     1441 gggtgaacgg gttcacggtg ccggggagcc ccccgcagac ccggcatccg ggctgcagag
     1501 gtgctccatg atccgcgcga cgacctcggg gttcctcggc agcgtgatgt ggcagtactg
     1561 ctccgggtgc cgggcaaagt tcaccgggtc tgccggtatc acgtccgtcc gcaccccggg
     1621 caggtgagag tcgatatggg ggacgatgcc gtcgccggcg taggtcgccc gccaccctcc
     1681 atccggagcg atctcccagg tccggccgtc gaagatcggg aagaacgccg gggtccgggt
     1741 gaggttcacg gcgaggacga tccggtaggc gatgtcgtcg cgggtgcctg cggcccggag
     1801 cgccgcgacg atccgactcc ggggccggaa ctcctgaacg atggtgtcgt cgccgggatc
     1861 gtagtccggc ggcacgaaga cacctgcaag actccggatc acctccgggc cccgcagggg
     1921 atcgttgaag agttcggcca tggccgagcc gttgttcggg ggaccgatcc cgacgagctg
     1981 ccggacgccc tcttctcgtg tgccgccgtc gaggacctcg aggaggtagc gggcgatgca
     2041 tgtccccatc gagtggcaga
//
LOCUS       DAOSII010000260         2053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_47130_length_2053_cov_49.799299, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000260 DAOSII010000000
VERSION     DAOSII010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47130_length_2053_cov_49.799299"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /gene="serS"
                     /locus_tag="PLV90_11070"
     CDS             complement(<1..1011)
                     /gene="serS"
                     /locus_tag="PLV90_11070"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOF97698.1"
                     /translation="MLELKFVRAHPEIVRADLTRRGDTGKFAWVDEVLEMDRRARELT
                     VAIGDLRNRRNVISREISQARKAGKDISELLAEAADLPARVKEAETEREALTEAVRCR
                     LMRLPNILHESVPVGKDDTENVEVRRWGEPAIPAFDLENHGALAVERGWADFERAAKI
                     AGAGFYFLKGRLALLDMALQRFAMDLLVERGYTPIIPPYMMNRAAYEGVTDLADFENV
                     MYKIEDEDEYLIATSEHPMAAMYSDEIFEEKDLPLRLAGLSPCFRREIGAHGLDTKGL
                     FRVHQFHKVEQFIYSTPEQSWPLHEELLANAEEIFQRLGLPYRVVLICTGDIGTVAAK
                     KYD"
     gene            1150..1599
                     /locus_tag="PLV90_11075"
     CDS             1150..1599
                     /locus_tag="PLV90_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97699.1"
                     /translation="MEKNQKIILVAGALITLGLLAIDVFFALIALVVVLILLMSFHIM
                     GETGSYLLIAADLAEDAREVIIANTGTATAQNIHVVLVPLDIEFDIPSLGPDERSVFG
                     LSSMISEAKAVVTYEDAAGRKHTRTYPLTALMAGRDPMKPMFPLFDR"
     gene            complement(1654..>2053)
                     /locus_tag="PLV90_11080"
     CDS             complement(1654..>2053)
                     /locus_tag="PLV90_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97700.1"
                     /translation="ADTAPVVGRMGLDMLRRAKREASGELYDQEYYEKKMIVLGKTDP
                     LPYRPDDPSKPIDTQICVLGEDGNLFEVMYTTTEIRIDSYLAPLAPEEAFDLYGYDVV
                     VMLYRALYEYAEKEEELTAALARTLEYIIS"
BASE COUNT          355 a          674 c          610 g          414 t
ORIGIN      
        1 gtcgtacttc ttcgcggcga cggtcccgat atcccccgtg cagatcagga cgacccggta
       61 ggggaggccg aggcgctgga agatctcttc ggcgttcgcg agcagttcct cgtggagggg
      121 ccaggactgc tccggcgtgc tgtagatgaa ctgctccacc ttgtggaact ggtggaccct
      181 aaagagcccc ttcgtatcga gcccgtgcgc cccgatctcg cgccggaagc acgggcttaa
      241 gcccgcaagc cggagcggga ggtctttttc ctcgaatatc tcgtcgctgt acatcgcggc
      301 catcgggtgc tcgctcgtcg ctatgaggta ctcgtcctcg tcctcgatct tgtacatgac
      361 gttctcgaag tcggcgagat cggtcacgcc ctcgtaggcg gcccggttca tcatgtaggg
      421 cgggatgatg ggggtgtagc cgcgctcgac gaggaggtcc atcgcgaacc gctggagcgc
      481 catatcgagg agggcaagcc tccccttcag gaagtagaac ccggccccgg cgatctttgc
      541 cgcgcgctcg aagtccgccc agccgcgctc gacggcgagc gcgccgtggt tctccaggtc
      601 aaacgccggt atggcgggct ctccccaccg cctgacctcg acgttctcgg tatcgtcctt
      661 gccgacgggc acgctctcgt gcagtatgtt cgggaggcgc atcaggcggc accgcaccgc
      721 ctcggtgagc gcttcacgct ccgtctccgc ctctttgacc cgcgcgggaa ggtccgccgc
      781 ctcggcaagg agttccgata tatcctttcc ggcctttctc gcctggctga tctcgcggga
      841 tatgacgttt ctccggttgc gcaggtcgcc gatcgccacg gtgagttccc gggccctccg
      901 gtccatctcc agcacctcgt cgacccaggc gaacttcccg gtatctcccc tcctggtgag
      961 gtctgccctg acgatctcgg ggtgtgcacg aacgaacttc aactcaagca tgctgtacgt
     1021 actttgccac ggcggtatat ggtcatgttg cccttctcca ggagttcagg agagaacgcc
     1081 cggtatgcac cctatctccc cgggtcgaaa acctaccctt ataattcccg gtgcgccaac
     1141 tcactaggca tggaaaagaa ccagaagatc atcctggtgg cgggcgctct catcacgctc
     1201 ggactgctcg ccatcgacgt cttctttgcc ctcatcgcgc tggtcgtcgt gctcatcctc
     1261 ctgatgagtt tccacatcat gggagagacc ggcagctacc tgctcatcgc cgcagacctc
     1321 gcagaggatg cccgggaggt catcatcgcc aacacgggca ccgcaacggc gcaaaacatc
     1381 cacgtcgtcc tcgtcccgct cgacatcgag ttcgatatac cgtcgctcgg gccggacgag
     1441 aggtccgtct tcggcctctc gtccatgata tccgaggcaa aagcggtcgt gacctacgag
     1501 gacgccgccg gccggaaaca cacacgcacg tatccgctca cggcgctcat ggccgggcgc
     1561 gacccgatga aacccatgtt cccgctcttt gaccggtgat gagagagcgc cgccgcaccc
     1621 ccgccggagg gcctgttcgg aaaagagggg ccctcaggag atgatatact caagcgtccg
     1681 cgcaagcgcg gccgtcagtt cctcctcttt ttcagcatac tcgtagagcg cccggtagag
     1741 catgacgacg acgtcgtagc cgtagagatc gaacgcttcc tctggcgcca ggggagcgag
     1801 gtatgagtcg atccggatct cggtggtcgt gtacatcacc tcaaagaggt tcccatcctc
     1861 gccaaggacg catatctggg tatcgatggg ttttgatgga tcgtcggggc gatagggaag
     1921 cggatcggtc ttgccgagga cgatcatctt cttctcgtag tactcctggt cgtagagctc
     1981 cccggaggcc tctcgttttg cacgccggag catatcaagg cccatccgcc ccacgaccgg
     2041 cgccgtatcg gcg
//
LOCUS       DAOSII010000261         2032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_47797_length_2032_cov_46.821447, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000261 DAOSII010000000
VERSION     DAOSII010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47797_length_2032_cov_46.821447"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2032
                     /locus_tag="PLV90_11085"
     CDS             <1..>2032
                     /locus_tag="PLV90_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOF97701.1"
                     /translation="FIVGDPKQSIYLFREADVAQFRHTRDMIERDFGGETIALDVNFR
                     SAPSVVEFTNAIFGALMAECGRPWEFLYEPLAPNRSDGAGSVEILLPQKVRDRAAGRR
                     AEADMAAKKIRAIVERGEKSVWRDGESRPAGYGDIAILLERRTNLAYYEWALARYGIP
                     YRVHAGLGFYERQELYDIYNILRFLVNDRDDVALYGALRSPYFGFSDARLFAIAGTSF
                     WERLQRSGDPDAIAAATTLRGWLSVARRLPPARLIRRVIDESGITVIFGGTAGGEQAV
                     ANVEKLIAIARASSVTLADLVEELGRSIDDGEREGDAPLDLTSSDAVSIMTVHAAKGL
                     EFPVVVVPDLAETPRAGGATIMVEGGLRLGVRIPNPAHDYEREDTPILQVLKAEYRQK
                     EEAERKRLFYVAATRAKDHLVLCGVRPDEAPETLAAGKTRMAWLAHCLGLSPEAYARG
                     EAVLEALRIPLTLDPASIPVDVGEAAPVCITVPDGIPAVAPAGILPRGTEEHVYSVSE
                     IERYIRDPAASRMPRFSTIPGPVTRGLAVHEVFRGREPAAVLSRYGMDVGRAEEYRDL
                     YERFLASPLMQGRIDDRCEVPFVARVNGVMFEGSIDRLVRRPDGSWVLIDYKTGDAGE
                     YAIQMTVYRHAAEQILGRPATPYLYFVDADRWVEVAVDEEQAFAAIERAVRG"
BASE COUNT          326 a          674 c          703 g          329 t
ORIGIN      
        1 tcttcatcgt cggcgacccg aaacagtcca tctacctctt ccgggaggcc gacgtcgccc
       61 agttccggca tacccgcgac atgatcgaac gggacttcgg cggggagacg atcgcgctcg
      121 acgtcaactt ccggagcgca ccctcagtcg tcgagttcac gaacgccata ttcggcgccc
      181 tgatggcgga gtgcggccgg ccctgggagt tcctctacga accgctggcg ccgaaccggt
      241 ctgacggcgc gggttcggtc gagatcctcc tcccccagaa ggtcagggac cgggcagcgg
      301 gccgccgggc cgaggcggat atggcggcaa agaagatccg ggccatcgtc gagcgggggg
      361 agaagtcggt ctggcgggat ggggagagcc ggccggccgg ctacggcgat atcgccatcc
      421 tcctcgagcg ccggacgaac ctcgcctatt acgagtgggc gcttgcgcgg tacggcatcc
      481 cctaccgcgt ccacgcgggc ctcgggttct acgagcgcca ggagctatac gacatctaca
      541 acatcctccg gttccttgta aacgaccggg acgacgtggc gctctacggc gcgctgcggt
      601 cgccttactt cgggttctcc gatgcccggc tctttgcgat cgcggggacc tccttctggg
      661 agcggctgca gcgatccggc gacccggacg ccatagcggc ggcgacgacc ctccggggct
      721 ggctctccgt cgcccgaagg ctcccgcccg cccgcctcat ccggcgggtc atcgatgagt
      781 cgggtatcac ggtcatcttc ggcgggacgg ccgggggcga gcaggcggtc gcaaacgtcg
      841 agaaactgat cgcgatcgcg cgggcatcct ccgtcacgct cgccgatctg gtcgaagaac
      901 tcggacgctc tatcgatgac ggcgagcgcg agggagatgc gccccttgac ctcacctcct
      961 cggacgcggt ctcgatcatg acggtccacg ccgcaaaggg gctagagttc cccgtcgtcg
     1021 tcgtcccgga ccttgcggag acgccgcggg ccggcggggc gaccatcatg gtcgagggcg
     1081 ggctcaggct cggtgtcagg ataccaaacc cggcacacga ctacgagcgc gaggatacgc
     1141 ccatcctcca ggtcctcaag gcagaatacc ggcagaaaga ggaggcggag aggaaacggc
     1201 tcttctacgt cgcggcaacg cgggcgaaag accacctggt cctctgcgga gtgaggccgg
     1261 atgaggcccc ggagaccctg gcggccggaa agacccggat ggcctggctc gcccactgtc
     1321 tcgggctctc cccggaggcc tatgcccggg gcgaagccgt ccttgaggcg ctccgtatcc
     1381 cgctcaccct cgatccggcg tcgataccgg tcgatgtggg ggaggccgcc ccggtctgca
     1441 taacggtccc ggacggtatc ccggccgtcg ctccggcggg tatcctccct cgcgggacag
     1501 aggagcacgt ctactcggta agcgagattg agcgctacat ccgcgatccg gcagcgtccc
     1561 gtatgccgcg gttctcgacc attccgggcc cggtaacccg tggtctcgcc gtccacgagg
     1621 tctttcgcgg ccgggagcct gcggcggtgc tatcgcgcta cggcatggat gtgggccggg
     1681 cggaggagta ccgcgacctc tacgagcggt tcctggcctc acccctgatg caggggcgta
     1741 tagacgatcg ctgcgaggtg ccgttcgttg cccgggtgaa cggtgttatg ttcgaggggt
     1801 ccatcgaccg tctggtgcgg cggccggacg ggtcctgggt gctcatcgac tacaagacgg
     1861 gagatgccgg ggagtacgcg atccagatga cggtctaccg gcatgccgcg gagcagatcc
     1921 tcggccggcc ggcgacaccc tacctctact tcgtcgacgc cgaccgctgg gtggaggtcg
     1981 cggtcgacga ggaacaggcc tttgccgcca tagagcgggc ggtcaggggg at
//
LOCUS       DAOSII010000262         2031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_47828_length_2031_cov_46.479757, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000262 DAOSII010000000
VERSION     DAOSII010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47828_length_2031_cov_46.479757"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(256..612)
                     /locus_tag="PLV90_11090"
     CDS             complement(256..612)
                     /locus_tag="PLV90_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOF97702.1"
                     /translation="MRDELFLRHILDEIMLLREIGRDLTYEDLLRDPVRQHAIVRAIE
                     IIGEATKNISEHQKEQHPEIPWRLMAGSRDKLIHAYFEVDWRIVWAILKTEIPVIEPR
                     VQAILLTLDTLTKNNE"
     gene            complement(605..925)
                     /locus_tag="PLV90_11095"
     CDS             complement(605..925)
                     /locus_tag="PLV90_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOF97703.1"
                     /translation="MTVPAQHGEKSNCVVQLEEVLPLLRERFGVAKIGIFGSTARGDD
                     RPESDVDVLVEFAPGQTTFRNFMELAFYLEDLFGRRVDLVTDQGLSRYLRPYVEQEVI
                     WCDA"
     gene            complement(1006..1332)
                     /locus_tag="PLV90_11100"
     CDS             complement(1006..1332)
                     /locus_tag="PLV90_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97704.1"
                     /translation="MKGSVIKLMMWGNLNTDMFAGLSPTSSARTSIRRSSAKYDIIRQ
                     EEAEVRCLEPVQCPGCNQRPACGTIAPPAAGRSLAMGKDVLDNPELVRKLLYELIEEK
                     ERKGEL"
     gene            1420..1599
                     /locus_tag="PLV90_11105"
     CDS             1420..1599
                     /locus_tag="PLV90_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97705.1"
                     /translation="MLATVNANSRKSGLKSAGYRCSVKGCDNPPERVDQNREWPLCSH
                     HYNCLKQKAAGTMQG"
BASE COUNT          422 a          619 c          577 g          413 t
ORIGIN      
        1 tgcggttctc ttcgacttcg tcgggctgtc gcagtacctc gaagaaaggc ctggctctcg
       61 cgtggatgtc gggccggcag cggcactccg gcccgagatc cgtggacatg tcgcccggga
      121 tctcctcttc gcgtgagagc atctacttcc cgggttctgc cgatgcaagc gatcgacatt
      181 ctgctaaccg ttacttagaa cgactcaggc gctcgctctc tacgggtgat cgagatttga
      241 agctcgacgg atccttcact cattattctt ggttaatgtg tcaagagtga gcagaattgc
      301 ctgtaccctc ggttcaataa ccgggatctc ggtcttgaga atagcccaga cgatcctcca
      361 gtcgacctca aaatatgcgt gaatcagttt gtcccgcgag ccggccatca accgccaggg
      421 aatctccgga tgctgttctt tctggtgctc ggagatgttc ttcgtagcct ctccgatgat
      481 ctcaatcgcc cggacgatcg cgtgttgccg caccgggtcg cgaagcaggt cctcgtatgt
      541 caggtctcta cctatctcgc ggagaagcat gatctcatca aggatatgac ggaggaagag
      601 ttcatcacgc atcacaccag atcacctcct gttcaacata gggccggagg tatcggctga
      661 gcccctgatc tgtgacgagg tccacccgac gcccgaagag atcctccagg tagaacgcga
      721 gttccatgaa gttccgaaac gtcgtctgcc ccggtgcaaa ctcgaccagc acatccacgt
      781 cgctctccgg ccggtcatca ccgcgggcgg tcgacccgaa gatgccgatc ttcgcgacac
      841 cgaaccgctc ccggagcagc gggagcactt cttcgagttg aacgacgcaa ttgctcttct
      901 ccccgtgttg cgccggaacc gtcatatggg ttctgctcgc ctcctgatcg tacctggggg
      961 tgaggagatg atgaagattg cccctcacag ggcagggggt gagcatcaga gctcaccctt
     1021 ccgctccttc tcctcgatca gttcgtacaa cagtttccgc acgagttcag ggttatcgag
     1081 cacatccttg cccatcgcca gcgagcgacc ggccgctggc ggagcaatag ttccgcacgc
     1141 ggggcgttga ttacagccgg ggcactgcac cggttcgagg catcgcacct ccgcttcttc
     1201 ctgccggatg atgtcgtact tggccgagga cctccttatc gatgtcctgg ccgatgaggt
     1261 gggcgaaagg ccggcgaaca tatcggtgtt gaggttcccc cacatcatca acttgatgac
     1321 cgaccctttc atcctctggt cgatgaggtt attgaagacc ctgatcgccc tgaccgggac
     1381 ggttctttgt catcatcgga ggacagcatc ccgccgcctg tgctcgccac tgtcaacgcc
     1441 aactcccgga aaagcggtct gaagagcgca ggataccggt gcagcgtcaa aggctgtgac
     1501 aatccgccgg agcgggtgga ccagaaccgc gagtggccac tctgcagcca ccactacaat
     1561 tgcctgaaac agaaggcggc cgggacaatg cagggatagc gggcgcacag tggatgtttt
     1621 caagggagac ccgccccggg ccaccgggag tccagcgggg acctccgatc cgggtgcgaa
     1681 ggcgacaaag atgacgagtg cgacatgccc cggcgccggg gtgttaggtg cccgacccag
     1741 gcagataacg ggatactctg atccctaaca gagcccatat cggcgcgtat tccgatgctg
     1801 gcaccgggaa agcggcccga tcctacagaa aaaagggttc tcagcgagac ttacaacccg
     1861 aaccatcaga acgcccgggg aggttacact cagtatccga accggcgatc gtagccggta
     1921 tgatccagcc actcgatgat gaccgcgttt acatcgccat cacgggctac agtatacatg
     1981 agccgccacg atcgggtgag attatacttc cagagattct tcagcggccc g
//
LOCUS       DAOSII010000263         1988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_49219_length_1988_cov_50.521469, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000263 DAOSII010000000
VERSION     DAOSII010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49219_length_1988_cov_50.521469"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PLV90_11110"
     CDS             <1..281
                     /locus_tag="PLV90_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOF97706.1"
                     /translation="ENVLVSIEDTGPGIPDSVKESFFHRNDRGWTEGCGDGLGLFIVS
                     TLIERYGGTIRVEDRVEGRPDLGAAFRFTLREVLHAVDDDDDDDEECG"
     gene            complement(471..830)
                     /locus_tag="PLV90_11115"
     CDS             complement(471..830)
                     /locus_tag="PLV90_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97707.1"
                     /translation="MSTYEHDDIFEAAIRILLEEDRCITVSFSPGGVSIRFPTTRKLA
                     EYLDIPHYYVLPRFGIMEHDGLIRRAERVGISTTAAGTVRLLAVMAERYRERAEEVLG
                     REVFSALQARAGAGPER"
     gene            complement(881..1114)
                     /locus_tag="PLV90_11120"
     CDS             complement(881..1114)
                     /locus_tag="PLV90_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97708.1"
                     /translation="MKIYARERQKVGTGVKQPRFRVVAVVEEKGEDKHLKVESTHFRK
                     IELEQIAKDIGAEVIYLEEMPEEERGEMKAEAA"
     gene            1208..>1988
                     /locus_tag="PLV90_11125"
     CDS             1208..>1988
                     /locus_tag="PLV90_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridylate kinase"
                     /protein_id="HOF97709.1"
                     /translation="MAERFEMESKLAGESLVRRGLMRKTAGVRQIRIMPDLNVIKIGG
                     HGVIDYGRDVIYPLIEEIGELSRNHKILIATGGGARTRHILDVGIDLGMPTGVLAELA
                     GKISEQNAIMMSLLFSRYNGVRISTGDLLNLPSLISLGMLPVVQGTPPYGLYEHPPEL
                     GSIPPHRTDTGAFLMAEVVGAKNCILGKNVNGLYTENPFINPDAEFIADITADELLKM
                     DLEDMVLEHMAVRLLRDAVHVKEIKIVNAHVPGNITKAVNGE"
BASE COUNT          374 a          602 c          615 g          397 t
ORIGIN      
        1 gcgagaacgt cctcgtctcc atcgaggata ccggtccggg tatccctgac tccgtgaagg
       61 agtcgttctt tcaccgaaac gatcgggggt ggaccgaggg gtgcggcgac gggctcggtc
      121 tcttcatcgt cagcaccctc atcgagcgtt acggcggtac gatacgggtg gaggaccggg
      181 tggaagggcg gccggacctc ggcgcggcgt tccggtttac cctccgcgag gtcctgcatg
      241 ccgtcgacga tgatgatgac gacgatgaag agtgcgggtg aagccctccg cgccccttcc
      301 ctcctccggg tgcgctaagc agcgtatctt ggctgcgata catccttctg cacccggggg
      361 atctcccgca acggccgggc cttacatgaa gggggagggt gccggcgccg catcgtcgac
      421 cgcgcagggg atcttttggt gcgtgccgcc atgcgtcgtg atagagtcct tcacctctcc
      481 ggtcccgcgc cggcgcgggc ctgcagcgcc gagaagacct ccctgccgag gacctcttct
      541 gcgcgttccc tgtagcgctc cgccatgacc gcgagaagcc gcaccgttcc cgccgccgtg
      601 gtggagatgc cgacccgctc cgccctgcgg atcagcccgt catgctccat gatcccgaac
      661 cgggggagaa cgtagtagtg gggaatatcg agatattcgg cgagtttccg ggttgtggga
      721 aaccgtatgg atacgccgcc cggggagaag ctgacggtta tgcaccggtc ttcttcaagg
      781 aggatccgga tggccgcctc gaatatgtcg tcgtgttcgt atgtggacat gcgatcgcag
      841 aagtactggt tttcggaaag aaaaaagagg gtcggtctgg ttacgccgct tccgccttca
      901 tctcgccccg ctcttcttcg ggcatctcct caagatagat gacctcggcg ccgatgtcct
      961 ttgcgatctg ttcgagctct atctttctga agtgagtgct ctccaccttc aggtgcttgt
     1021 cctcgccctt ctcttcgacg acggcgacga cgcggaaccg gggttgtttc acgccggtgc
     1081 cgactttctg acgctcacgt gcatagatct tcatggtctc cacatcccaa taccaggtgg
     1141 tatatcacca ggtatatccg gaagatactt aatactttcc ccgtgtacac tatggtgagg
     1201 tttgatcatg gcagaacggt ttgagatgga gtcgaaactt gcgggcgaga gccttgtccg
     1261 gcggggcctg atgcgaaaga ctgccggtgt ccggcagatc aggatcatgc ccgacctgaa
     1321 tgtgatcaag atcggcggtc acggtgtcat cgattatggg cgggacgtga tctacccgct
     1381 gatcgaagag atcggagaac tctcgcggaa ccacaagatc ctgatcgcca ccggcggcgg
     1441 cgcccggacg cggcacatcc tcgatgtcgg gatcgacctc ggcatgccga ccggggtgct
     1501 tgcggaactc gcgggcaaga tcagcgagca gaacgcgatc atgatgtccc tcctcttctc
     1561 gaggtacaac ggcgtccgga tcagcactgg cgacctctta aacctcccct cgctcatatc
     1621 gctcgggatg ctgccggtcg tccagggcac cccgccctac ggcctctacg agcacccgcc
     1681 ggaactcggg agcatcccgc cgcaccggac ggataccggc gcgttcctga tggccgaggt
     1741 cgtgggcgca aagaactgca tcctcgggaa gaacgtaaat gggctctata cggagaaccc
     1801 gttcataaac cccgatgccg agttcatcgc ggatatcacg gccgatgaac tcctgaagat
     1861 ggacctggag gacatggtgc tcgagcatat ggcggtgcgg ctcctccgcg acgccgtcca
     1921 cgtgaaagag atcaagatag tgaacgccca tgtgccgggc aacataacaa aagccgtgaa
     1981 cggggagc
//
LOCUS       DAOSII010000264         1981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_49459_length_1981_cov_42.766874, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000264 DAOSII010000000
VERSION     DAOSII010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49459_length_1981_cov_42.766874"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /gene="nth"
                     /locus_tag="PLV90_11130"
     CDS             complement(<1..628)
                     /gene="nth"
                     /locus_tag="PLV90_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOF97710.1"
                     /translation="MNRKTACEAHRRLLAEYPVAGGRRHFLDFRNPYETLVLTILSAQ
                     TTDRAVNAIRDALFSRYPTPEALARAEPEDVEPIIRTIGFYRTKARHIVGAARKVVGE
                     FGGEVPRTMEELQSLPGVGRKTANIILSQAFGIDVGIAVDTHVRRVSGRMGFTDSTNP
                     VIIERDLMALFPEEVWGDINYLLIRHGRAVCTARNPKHDRCVVADLCRC"
     gene            complement(635..709)
                     /locus_tag="PLV90_11135"
     tRNA            complement(635..709)
                     /locus_tag="PLV90_11135"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(671..673),aa:Pro,seq:tgg)
     gene            complement(761..1465)
                     /gene="pyrH"
                     /locus_tag="PLV90_11140"
     CDS             complement(761..1465)
                     /gene="pyrH"
                     /locus_tag="PLV90_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOF97711.1"
                     /translation="MKKIVISLGGSVLVPSLESNNIGRYVSVLRKLAGRCRVFVVVGG
                     GGEARRYIQVARGLGAGEATADELGIQVTRLNARLLVAGLGDAAYPRVAENYTEAREF
                     ALAGKVVVMGGITPAQTTDAVSAVLAESIGADLLINATSVDGIYSADPKKDPAAVRYE
                     RLTPEGLLAIITRERMGAGANTVLDIVAGKVVERSGIPLLVLDGRDPENLYRAIVEGA
                     FVGTIVSEEHVNPLPA"
     gene            1507..1665
                     /locus_tag="PLV90_11145"
     CDS             1507..1665
                     /locus_tag="PLV90_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97712.1"
                     /translation="MNRLHCPVCNATEVFEATGGCTGRLYRCKRCGYRGALVIEYDDE
                     EHDGHQSD"
     gene            1701..>1981
                     /locus_tag="PLV90_11150"
     CDS             1701..>1981
                     /locus_tag="PLV90_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOF97713.1"
                     /translation="MHEARLYQKLEEDTVRCSLCPHRCTIRDGKQGICGVRINHGGTL
                     YAATFGKVIAEAVDPIEKKPLYHFLPGSLSYSLGTIGCNFHCEHCQNWH"
BASE COUNT          378 a          662 c          601 g          340 t
ORIGIN      
        1 aacaccggca gaggtccgcg acgacgcacc ggtcgtgctt cgggttcctc gccgtgcaga
       61 cggcgcgccc gtggcggatc aggaggtagt tgatatcccc ccagacctcc tcggggaaga
      121 gggccataag atcgcgctcg ataataacag ggttcgtgct gtccgtaaac cccattctcc
      181 cggagacccg gcggacgtgg gtgtcgaccg cgatgccgac gtcgatcccg aacgcctggg
      241 agaggatgat gtttgcggtc tttcgcccca caccgggaag cgactggagc tcctccatcg
      301 tccgcgggac ctcgccgccg aactcgccga caaccttcct tgccgccccg acgatatggc
      361 gcgccttcgt gcgataaaac ccgatcgtcc ggatgatcgg ctccacgtcc tcgggctccg
      421 cgcgggcgag cgcctccggc gtcgggtagc gagagaagag agcgtcgcgg atggcgttga
      481 ccgcgcggtc ggtcgtctgc gccgagagga tcgtgaggac gagcgtctcg tacggattac
      541 gaaagtcgag aaaatggcgt cgaccgccgg cgacgggata ctcggcaaga agacggcggt
      601 gtgcctcgca ggcggtcttt cggttcatgg ttgatggggc agggcagatt cgaactgccg
      661 tcaaagcgtc ccaaacgctc taggatggac caggctaccc tactgccccg taccactata
      721 cgttggaaag accggcaaaa atagttaccg atggagggga tcaggccggc agggggttca
      781 cgtgctcttc gctcacgatg gtgccgacaa acgctccctc cacgatcgcc cggtagaggt
      841 tctccgggtc gcggccgtca agcaccagga gcgggatccc ggaccgctcg acgaccttcc
      901 cggccacgat atcgagcacc gtgttcgcac ccgcacccat cctctcgcgg gtgatgatag
      961 caaggaggcc ctccggcgtg aggcgctcat acctcaccgc ggcgggatct ttcttcgggt
     1021 ccgcgctgta gataccgtcg accgacgtgg cgttgatgag gagatcggcg ccgatgctct
     1081 cggcaaggac cgcggagacc gcgtcggtcg tctgcgccgg cgtgatgccg cccatgacga
     1141 cgacctttcc cgccagcgcg aactcccgcg cctccgtgta gttctcggca acgcgcgggt
     1201 aggccgcatc cccgagccct gccacgagaa ggcgcgcgtt cagccgggtc acctggatgc
     1261 cgagttcgtc ggccgtcgcc tcgccggcac caagcccgcg ggcgacctgg atgtaccggc
     1321 gtgcctcccc gccgccgccg acgacgacaa agaccctgca tcttccggcg agctttcgca
     1381 agacagagac atatcgaccg atattattgg attcaagcga ggggaccagg accgatcccc
     1441 ccagggatat cacgatcttc ttcatcctac cctactatta tccccgatat cacccatata
     1501 cttgttgtga acaggctgca ttgccccgta tgcaacgcga cagaggtctt cgaggccacc
     1561 ggaggctgca ccggcaggct ttaccggtgc aaacgctgcg ggtatcgcgg agcgctcgtc
     1621 atcgagtacg acgacgagga gcacgacggg caccagagcg attaataccc tgcgcccccc
     1681 catcctcatg cggtaagcct atgcacgagg cgcgactcta ccagaaactt gaagaggata
     1741 ccgtccgctg ttcgctctgc ccgcaccggt gcaccatacg ggacggcaaa caggggatct
     1801 gcggcgttcg catcaaccac ggcggcacgc tctatgccgc caccttcggc aaagtcatcg
     1861 ccgaagcggt cgaccccatc gagaagaaac cgctctacca cttcctgccc ggatctctct
     1921 cttactccct cgggaccatc gggtgcaact tccactgcga gcactgccag aactggcaca
     1981 t
//
LOCUS       DAOSII010000265         1972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_49755_length_1972_cov_38.414710, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000265 DAOSII010000000
VERSION     DAOSII010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49755_length_1972_cov_38.414710"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..1620
                     /locus_tag="PLV90_11155"
     CDS             67..1620
                     /locus_tag="PLV90_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF97714.1"
                     /translation="MAESPERSSELPGMAESYWLATSPETAFPPLGGDGQADVTVIGG
                     GIVGVTTAFLLKRAGLTVILVDAYRIVTGTTGHTTAKVTSLHQLIYADLIDRFGSTKA
                     RQYADANQAAIETVASLVREYAIPCNFERKPAYTFAESADDREKVATEADAAKSLGLP
                     AAFTEEVPLPVETHGAVRFADQAQFHPRNYLLLLAGHIPGEGSVIFEMTRAVSIDEQD
                     GGVTVRTDRGLLRSDHAVLATHYPIYDIPGAYFARMQPSKSYALGLRIDEPFPNGMFI
                     NTSGAVHSWRSQPTAGGEIAIVTGAEHDTGKVTDTRACYRNLEAYARSVYPVRSIDYH
                     WSAQDYMTPDGVPYIGPLAPGHGRVFVATGFGKWGMTNGTAAGMILADLIQDRTNPWA
                     EVFDPARFGKGPAPPAWVRGALQSAGETVETDTDRLTREAATIPPGEGKVIEIGGRKV
                     AVSRDEHGALHTLDPTCMHMGCTVAWNNAEKSWDCPCHGSRYDALGKVIESPTVKDLA
                     RIEVVRKRD"
     gene            1722..1958
                     /locus_tag="PLV90_11160"
     CDS             1722..1958
                     /locus_tag="PLV90_11160"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOF97715.1"
                     /translation="MRIGVCGIACEICPKMSSGTCPRPGVGCIPKENPFCSIATCAYR
                     KGMRYCFACPDFPCGLTGQGPVKEGFCRHISGKD"
BASE COUNT          373 a          628 c          646 g          325 t
ORIGIN      
        1 tcgccgtaac cctgcacaac cattatgtgg gtgccggggc atccaccccg ttttgaggag
       61 aggtacatgg cagagtcacc ggaacggagc agcgaactgc ccggcatggc agagtcatat
      121 tggttagcca catcgccgga gacggcgttt ccgcccctcg gcggggacgg gcaggcggac
      181 gtcacggtga tcggcggagg gatagtgggt gtcaccacgg cattcctgct gaaacgggca
      241 gggctgaccg tcatcctcgt cgatgcgtac cggatcgtca cgggcaccac cggccacacg
      301 acggcgaagg tcacgtcgct ccaccagctg atctacgccg atctcatcga ccggttcggg
      361 agcacaaagg cccggcagta cgcggatgcg aaccaggcag cgatcgagac ggttgcgtcc
      421 ctcgtccggg agtatgccat cccgtgcaac ttcgagcgaa aacccgccta tacttttgcg
      481 gaatcggcgg acgaccgtga gaaggttgcc accgaggcgg atgcggcaaa gagtctcggc
      541 ctgccggccg cgttcaccga ggaagtgccg ctccccgtgg agacccacgg agcggtgcgg
      601 tttgccgacc aggcgcagtt ccacccgcga aactacctcc tgctgctcgc cgggcacatc
      661 cccggagagg gaagcgtcat atttgagatg acccgcgccg tgagtatcga tgagcaggac
      721 ggcggggtga cggtcaggac cgaccggggt ttgcttcgct ccgaccatgc ggtccttgcc
      781 acgcactacc ccatctacga catccccggt gcatacttcg cccggatgca accgtcgaag
      841 tcctatgccc tgggcctcag gatcgacgaa cccttcccga acggtatgtt catcaacacc
      901 tccggcgccg tccactcctg gcggtcacaa cccaccgccg gcggtgagat cgccatcgtc
      961 accggcgcgg agcacgatac cgggaaggtg accgataccc gggcatgcta tcggaacctc
     1021 gaggcgtatg cccggtcggt ctacccggtc cgttccatcg actaccactg gtcggcgcag
     1081 gactacatga cgcccgacgg cgtcccctac atcggcccgc tcgccccggg gcatggccgc
     1141 gtcttcgtcg ccacgggttt cgggaagtgg gggatgacca acggcacggc ggcggggatg
     1201 atccttgccg acctgatcca ggatcgcacg aacccctggg cggaggtctt cgatcctgca
     1261 cggttcggga aagggcctgc ccctcccgca tgggtcagag gggcgctcca gtctgccggg
     1321 gagacggtcg agaccgatac cgaccgcctc acccgggagg ccgcgacgat acctcccggg
     1381 gaggggaagg tcatcgagat cggcgggcga aaggttgccg tctcccgcga cgagcatggc
     1441 gcgctccata cgctggaccc gacctgcatg cacatgggct gcaccgtcgc ctggaacaac
     1501 gcggaaaagt cctgggactg cccctgccac ggctcacgct atgacgccct gggaaaggtg
     1561 atcgagagcc cgacggtgaa ggatcttgca aggatagagg ttgtgcggaa gcgggactga
     1621 ggggatgcac ggggggggcg gtgcagatct gctcttctcc caacttctcc ggcggtagag
     1681 tgcataaggc ttaacagggc gggcttatag acgatagatc gatgaggatt ggtgtgtgcg
     1741 gcatcgcctg cgagatctgt ccgaagatgt ccagcggcac ctgcccccgc cccggggtag
     1801 ggtgcatccc gaaagagaac ccgttctgct cgatcgcgac ctgcgcctac aggaaaggga
     1861 tgagatactg ttttgcctgc cccgatttcc cctgcggact tacgggccag ggcccggtga
     1921 aggaaggatt ctgccggcat atcagcggga aggactgatc cggccccata cc
//
LOCUS       DAOSII010000266         1970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_49839_length_1970_cov_58.654830, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000266 DAOSII010000000
VERSION     DAOSII010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49839_length_1970_cov_58.654830"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1841
                     /gene="fdhF"
                     /locus_tag="PLV90_11165"
     CDS             <1..1841
                     /gene="fdhF"
                     /locus_tag="PLV90_11165"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HOF97716.1"
                     /translation="WDEAYDLIAQKFKSYKPDEIACLSSARVSNEENYLLMKLARGVF
                     KTRHIDHCARLCHSSTVAGLAASFGSGAMTNSIGDIAESKCVFVLGSNTFEQHPLIGR
                     KIVQAKLNGGKIIVFDPRYTPTAKQADLYASFYSGTDVAILNCLMGEIIRNGWEDKEW
                     VSTRAKGYEELKAVVLKDDYLPENVEKISGISAQDLRTAAEWIGTAESAALLYSMGIT
                     QHTVGVDNVKSTANIQMLTGNIGKRGGGVNALRGQNNVQGACDMGALPVVFTGYQKVI
                     DPAAHKKFADAWGFPDGICEPKNGYEVTVMMDVLTDNPGELKCMYIMGENPMLSDPDL
                     NHVRHALESVEFLVVQDIFLTETAELADVILPATCYAEKEGTQTSTERRVQMWRKAQD
                     PPGEAKVDWQILCEVAAAMGYAEQFPYHSAEEIFNEVAALTPSYHGMTYERLNKPEAL
                     HWPCPTLEHPGTPILHIGKCSHPDGMGVMHAIEWKPPAEVPDAEYPYILTTGRCIWHW
                     HTGSMTRRSETLDTEVPTGWIEINTEDAKELGIQDKEMVRAMTRRGSVNAPAKVTDEI
                     KKGVMFMPFHFAECAANVLTHNALDPIAKIPEFKACAVKVEKITEA"
     gene            1844..>1970
                     /locus_tag="PLV90_11170"
     CDS             1844..>1970
                     /locus_tag="PLV90_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase/dehydrogenase beta
                     subunit N-terminal domain-containing protein"
                     /protein_id="HOF97717.1"
                     /translation="MAAKGDTYYAWAADAACQERGECGGAVTALLTHALKSGMVDA"
BASE COUNT          422 a          657 c          569 g          322 t
ORIGIN      
        1 cctgggatga ggcctacgac ctgatcgcgc agaagttcaa gtcctacaag cccgatgaga
       61 tcgcctgcct ctcctccgct cgtgtctcaa atgaagagaa ctacctgctg atgaaactgg
      121 cacgtggtgt cttcaagacg cgtcatatcg accactgcgc ccggctctgc cactcgtcca
      181 ccgtcgccgg ccttgctgca tcgttcggtt ccggtgcgat gacgaactcc atcggcgaca
      241 tcgccgagtc caagtgcgtc ttcgttctcg ggtccaacac attcgagcag cacccgctca
      301 tcggccgcaa gatcgtgcag gccaaactga acggtggaaa gatcatcgtc tttgacccgc
      361 gttacacccc gaccgccaag caggctgacc tctacgcctc cttctactcc ggcaccgatg
      421 tggccatcct caactgcctg atgggcgaga tcatccggaa cggctgggag gacaaggaat
      481 gggtctcgac ccgtgcaaag ggatacgagg aactcaaagc ggtcgtgctc aaggatgact
      541 acctccctga gaacgtcgag aagatctccg gcatatccgc tcaggacctc aggaccgcag
      601 ccgagtggat cggtaccgcc gagtccgccg cgctcctcta ctcgatgggc atcacccagc
      661 acaccgtcgg cgtcgataac gtcaagtcga ctgcgaacat ccagatgctg accggaaaca
      721 tcggcaagcg tggtggcggc gtgaacgctc tccgtggcca gaacaacgtt cagggcgcct
      781 gcgacatggg tgcgctcccg gtcgtcttca ccggctacca gaaggtcatc gacccggccg
      841 cccacaagaa gttcgccgat gcctggggct tccccgacgg tatctgcgag ccgaagaacg
      901 gctacgaggt caccgtcatg atggacgtcc tgaccgacaa ccccggcgaa ctcaagtgca
      961 tgtacatcat gggcgagaac ccgatgctct ccgacccgga cctcaaccac gtccggcatg
     1021 ccctcgagag cgtggagttc ctggtcgtcc aggatatctt cctgaccgag accgccgaac
     1081 ttgccgatgt cattctccct gccacctgct acgcggagaa ggagggcacc cagacatcca
     1141 ccgagcggcg tgtccagatg tggcggaagg cccaggaccc gccgggcgag gcgaaggtcg
     1201 actggcagat cctctgcgag gtggcagccg ccatgggcta cgcagaacag ttcccctacc
     1261 atagtgctga ggagatcttc aacgaggtcg ccgccctcac gccatcctac cacggtatga
     1321 cctacgagcg gctgaacaag cccgaagcac tccactggcc ctgcccgacc ctggaacacc
     1381 ccggcacgcc catcctgcac atcggcaagt gttcccaccc cgacggtatg ggcgtgatgc
     1441 acgccatcga gtggaaaccg ccggcggaag tccccgacgc ggagtacccc tacatcctta
     1501 ccaccgggcg ctgtatctgg cactggcaca ccgggtccat gacccgccgc tctgagaccc
     1561 tcgacaccga ggttccgacg ggctggatcg agatcaacac cgaagatgca aaagaactcg
     1621 gcatccagga taaggagatg gtccgcgcga tgacccgccg tggatccgtc aacgcgcccg
     1681 cgaaggtgac cgacgagatc aagaagggcg tcatgttcat gccgttccac ttcgccgaat
     1741 gcgcggcaaa cgtcctgacc cacaacgccc tcgacccgat cgcaaagatc cccgagttca
     1801 aggcctgtgc cgtgaaggtc gagaagatca cggaggcctg aagatggcag cgaaaggaga
     1861 tacatactac gcatgggcag ccgacgccgc ctgccaggag aggggcgagt gcggcggagc
     1921 ggtcaccgct ctgctgacgc acgcgctcaa gtccggcatg gtggacgctg
//
LOCUS       DAOSII010000267         1956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_50336_length_1956_cov_43.498159, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000267 DAOSII010000000
VERSION     DAOSII010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50336_length_1956_cov_43.498159"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /gene="cbiM"
                     /locus_tag="PLV90_11175"
     CDS             complement(<1..467)
                     /gene="cbiM"
                     /locus_tag="PLV90_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transporter CbiM"
                     /protein_id="HOF97718.1"
                     /translation="MHIPDAFIPIEQALAYWVIALIFIALSLRWARRSMVEEKVPLVA
                     VLAAGIFAIQALEIPIPWGTSGHMIGAALAAIVLGSPFAGVFVLTLVILVQALVFGDG
                     GITVMGANIIDMGVVGGFVGYYGYTAVKGIAKNPYVAAFIAGWASLFISATLVA"
     gene            complement(531..1679)
                     /locus_tag="PLV90_11180"
     CDS             complement(531..1679)
                     /locus_tag="PLV90_11180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOF97719.1"
                     /translation="MWDVVVVGAGPAGSAAARACAEEGLTTLCIEEHGTIGYPVQCAG
                     LLSASALAECDVSKRSVTNEVSGARMVSDLGGELLFDARTTKAYVVDRCLLDREMAEN
                     AANAGAEFRLKTSASGIRGTALLTRGIRGREEIPFRLLIAADGPRSSIARMLGLRRAA
                     TYLAGVQADVPCAVDPRYVELHPNASPDFFGWVIPVTATRARVGLCAREGAKDHFERF
                     IARYGETTLNLTSGVIPLGVMPQTYGSRALFVGDAAGFAKPTSGGGVYTGVRSAKHAA
                     AVAAACLARGAFDDGSLRDYERRWREDFGRELDIGMKALRMRQKMTPQEIDRLCRVLN
                     DPDIIETIVEHGDMDRPGTLIRKLALKPRLARAMGILFASGVRQILTG"
BASE COUNT          360 a          654 c          617 g          325 t
ORIGIN      
        1 gcaacgaggg tcgcggatat gaagagcgac gcccacccgg cgatgaacgc ggcgacatat
       61 gggttctttg cgataccctt gacggctgtg tagccgtagt agccgacgaa cccgccgacg
      121 acgcccatgt cgatgatgtt cgcgcccatg acggtgatcc cgccgtcgcc gaatacgagg
      181 gcctgcacga ggatgaccag cgtcaggaca aagacccccg caaacggcga accgaggacg
      241 atcgccgcga gtgccgcccc gatcatatgg ccgctcgtcc cccaggggat ggggatctcc
      301 agcgcctgga tggcaaagat ccctgccgca agcacggcga ccagcggcac cttctcctcc
      361 accatcgacc gccgcgccca ccggagggag agagcgatga agatgagggc gatgacccag
      421 taggccaggg cctgctctat cggtatgaat gcatcaggta tatgcatggc acaccccggc
      481 gtgttggaat acgtattcgt atataggtat tcccaggcga gaaagttctc tcaacccgta
      541 agaatctggc ggactcccga tgcgaagagt atgcccatcg ccctggccag ccggggtttc
      601 agggcgagtt tgcgtatcag ggtgcccggc cggtccatat cgccgtgctc cacgatcgtc
      661 tcgatgatat cggggtcgtt tagtacccgg cagaggcggt cgatctcctg cggcgtcatc
      721 ttctgccgca tacggagcgc cttcatgccg atatcgagtt ctcgcccgaa atcctccctc
      781 cagcgccgct cgtagtcccg gaggctcccg tcatcgaacg ccccgcgcgc aaggcaggcc
      841 gccgcgactg cggccgcgtg ttttgccgac cggacgccgg tgtagacgcc gccgcccgac
      901 gtgggtttgg caaaccccgc tgcatcgccg acgaagagcg cacggctgcc gtaagtctgc
      961 ggcataaccc cgagcggtat gacgccgctc gtgaggttga gcgtggtctc cccgtagcgg
     1021 gcgatgaacc gctcgaagtg gtcctttgcc ccctctctcg cgcagagccc gacgcgcgcc
     1081 cgggtcgccg tgaccgggat cacccacccg aagaagtcgg gcgaggcgtt cgggtgcagt
     1141 tcgacgtagc gcgggtccac cgcacacggc acgtccgcct gcacgccggc gaggtaggtc
     1201 gccgcccgcc ggaggccgag catccgggcg atggaactcc gtggtccgtc ggcggcgata
     1261 aggaggcgga aagggatctc ttcgcgcccg cggattcccc gggtcaggag agcggtgccc
     1321 ctgatgcccg aggcgctcgt ctttaagcgg aactccgccc cggcattggc ggcattctcc
     1381 gccatctccc ggtcgaggag gcaccgatcc acgacgtagg ccttcgtggt ccgcgcatca
     1441 aagaggagct ccccgccgag gtccgagacc atccgtgccc cgctcacctc gttcgtgacc
     1501 gaacgttttg agacatcgca ctcggcaagc gcggatgcgg agaggagccc cgcgcactgg
     1561 accgggtagc cgatcgtgcc gtgctcctcg atacagagcg tcgtaagccc ttcctcggca
     1621 cacgcccgcg cggcagcgct cccggcaggc cctgcgccca caacaacaac atcccagatc
     1681 agactaccat gcctcgcgta tacgggaccc cggcgatccc tttcgaataa taggggccgg
     1741 cgggtataaa tgggcacggc cggcggtcgt gtctttcacc tatccgggat accccgccac
     1801 gaaccggccc cccatctcca gcaggttgcc gccctcatag atgcgtattt gacggatcat
     1861 gccccaggta gtaggagatg cctgatatac gggagttcaa gtggaagcag tgcctgatcg
     1921 tgcgtgccga cgtcaggatg agctgcggca agaagt
//
LOCUS       DAOSII010000268         1951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_50509_length_1951_cov_25.233122, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000268 DAOSII010000000
VERSION     DAOSII010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50509_length_1951_cov_25.233122"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..1652)
                     /locus_tag="PLV90_11185"
     CDS             complement(216..1652)
                     /locus_tag="PLV90_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97720.1"
                     /translation="MRRLLLLPLLIAAVCVASVAAAGITLSTGQSDYAVLVGEEAAFP
                     IAVENTFGADVTGVLARNVRFVPEGGEAATHSQSRAITLFSDTREITVSAGPTVMPGT
                     FFFDIAFEYGNAPRTRVVLPEIRVHVAGSAAEEEGEQGPQVSSASTEAGSGGAQGGTS
                     STRQPGQIMQDTGSLREQAAREQDRMQALRSGLLAGLDDDPPVREIHAVLTSRGYTRV
                     AATINPVDEATGSFSFEYRNGAGDTVRAEGAVVNGSAAYAAYATEKSLVVPDAVTANE
                     TYKRYAGELADAGFFPATSSMNATASASSVGVGFIDGQNRTARIVATLSGGEVRSVDI
                     EEPSDLLPLAGAGALCIILLIAYLVRRGAPKAPEPPVTPIPADSRGEALRLLAAAREE
                     FDRGERTGAYGTAGRALRSYVSSVYGTGAELADGDVLRLLEESGRETGAASSLLGRCA
                     AVSYAGSPPLDEGFRAVVGEIEAFIRRD"
     gene            complement(1649..>1951)
                     /locus_tag="PLV90_11190"
     CDS             complement(1649..>1951)
                     /locus_tag="PLV90_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotolerance regulator BatA"
                     /protein_id="HOF97721.1"
                     /translation="ARERNVPVYTVGIGSANPPAGTQEYAALDEETLRAIARTTGGRY
                     YAAVDEGTLAAVYAGLTDEIPREPKETSIRDLFFAGALGVLLLEVYLRYGRGRILP"
BASE COUNT          317 a          679 c          654 g          301 t
ORIGIN      
        1 cggcgaccgc gcgggccagg cggccggttc cccgggattc aaggttgtcc gtatcgtcaa
       61 gagcaagaag tatggtcatg atacgccttt ttcctgataa acctgggaga atgaagataa
      121 aaaagatgct gaagcagggc gcgtatcaac ctggttttgt taacccggtg ccggataacg
      181 atatcgggtt aacccttccc cggagcgccg ccggatcaat ctcgcctgat aaaggcctcg
      241 atctccccga cgaccgcgcg gaacccttcg tccaggggtg gtgacccggc atacgagacg
      301 gccgcgcacc gcccgaggag ggacgatgcc gcaccggtct cgcgcccgct ctcctcaagc
      361 aggcgcagga catccccgtc cgcgagttcc gcgccggtcc cgtatacgga cgatacgtac
      421 gaccgcagcg ccctgccggc cgttccatac gctcccgtcc gctcgccgcg gtcaaactcc
      481 tcccgtgccg ccgcaaggag gcggagggcc tcgccgcggg agtcggcggg gatgggggtg
      541 accggcggct ccggcgcctt cggtgctccc cgccggacga ggtaggcgat caggaggatg
      601 atgcagagcg cgcctgcgcc ggcaaggggg aggaggtccg acggttcttc gatgtccacc
      661 gaccgcacct cccccccgga gagcgtggcg acgatcctcg ccgtccggtt ctggccgtcg
      721 atgaacccta cgccgacaga ggaggcgctc gccgtcgcgt tcatcgatga cgtcgccggg
      781 aagaaaccgg cgtcggcaag ttccccggca taccgtttgt aggtctcgtt tgccgtgacc
      841 gcatccggca ccacgagcga cttttcggtg gcatacgccg cgtaggccgc agaaccgttc
      901 accaccgcgc cctccgcgcg caccgtatcc ccggcgccgt tgcgatactc aaacgagaac
      961 gatcccgtgg cctcatccac cgggttgatc gtcgccgcca cccgggtata cccgcgcgac
     1021 gtgaggacgg cgtggatctc ccggacaggc gggtcgtcat cgagcccggc aagcagacct
     1081 gaccgcagcg cctgcatgcg gtcctgctcc cgtgccgcct gctcccggag cgaacccgtg
     1141 tcctgcatga tctgcccggg ctgccgcgtg gacgaagtcc ctccctgcgc ccccccggac
     1201 cccgcctccg tcgaggcgga actcacctgc gggccctgct ccccctcttc ttctgcggcc
     1261 gaaccggcga cgtggacccg gatctcgggg aggacgacgc gggtgcgcgg ggcgttcccg
     1321 tactcaaacg cgatatcgaa gaagaacgtg ccgggcatta ccgtgggccc ggcgctgacg
     1381 gttatctccc gggtatccga aaagagggtg atcgcccggg actgggagtg ggtggccgcc
     1441 tcaccgccct ccggcacgaa ccgcacgttc cgggcgagca ctccggtgac gtcggctccg
     1501 aacgtgttct cgacggcgat gggaaacgcg gcctcttccc cgaccagcac ggcgtagtcg
     1561 gactgcccgg tcgagagggt gatgccggcg gccgcgacgg aggccacgca gacggcggcg
     1621 atgagaaggg ggaggaggag aaggcgcctc acgggaggat cctcccccga ccgtagcgga
     1681 ggtagacctc aaggaggagg accccgagcg ccccggcaaa gaagagatcg cggatgctcg
     1741 tctcttttgg ctcgcgcggg atctcgtcgg tcagaccggc atagaccgcg gcaagcgtcc
     1801 cctcgtcgac cgccgcgtag taccttcccc cggtcgtccg ggcgatcgcc cggagggtct
     1861 cttcgtcgag cgcggcgtac tcctgggtgc ccgccggcgg gtttgccgag ccgatgccca
     1921 cggtgtagac cgggacgttc cgctcgcggg c
//
LOCUS       DAOSII010000269         1919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_51729_length_1919_cov_65.614270, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000269 DAOSII010000000
VERSION     DAOSII010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51729_length_1919_cov_65.614270"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            536..>1919
                     /locus_tag="PLV90_11195"
     CDS             536..>1919
                     /locus_tag="PLV90_11195"
                     /inference="COORDINATES: protein motif:HMM:NF023252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3344 domain-containing protein"
                     /protein_id="HOF97722.1"
                     /translation="MASIFMTVPVSAIYDFEGISLDTAAQGEVRGDVLTFGTYGLAAP
                     PLECAFSLPAKPVWARVYTGVWGGTERYTGWEELSVNNGVPVRRDLFGKDDRHPETLV
                     SGYGVYWVAHDCTDQLRGGKNTVTLTTSRGQPESRIDGRVYGIFVVAVVEDAARPVTR
                     YWIAEGNENLHGEGWSGTNPTRHDTCEVVFEKAGAIEKGAADLTVVLLASTKGQPDYI
                     LFNGEDLGVLVTDLSLYPEGARDIGNEHIGDATGGAGVEARYVDVETFDVGRLLKETN
                     RIVFEQGRDLDGDGEISTTGSKPEGEDYIHPVSAILTVQRPGASPAPDFAVDTPVVTG
                     AYAGEEATIRAMVRNYGVRPDGKIAVAFSVDGSVVKTVEVEPARSGVQQVETGVTLAE
                     GRHTVAVRVDAAGDADPADNEASMQLTVGTVPDLSVAIGAPIRGGLEAGAQAAPTQQA
                     PAPFFALFAGL"
BASE COUNT          401 a          575 c          606 g          337 t
ORIGIN      
        1 ttcatcacag cgtgttaaat atattatcat ttgtatcatt tattcgaagt tattttaaaa
       61 cacggcctat ggaatgggaa taaacacgaa atacatatta acatccatgg ggctctcccg
      121 gagcatcgat gaaggcctgc tcttgtcggg aaggacaaac gcattccacc cgccggggct
      181 catgcacccc ggcaggcgcg cttccgatct gcaggagttt caggccttcg aggaagtttt
      241 cggcagcggt gcgcaggggc aatgtgccgg aggaccggaa cctgcaggta atcctatccg
      301 gataatcgag caaatcaggc tggggcacaa gtagaaaatg atctggctgt ttataagggt
      361 ctgatggaaa taactccaat ttgaggttct tttgcggcac ctgcggggtt gtgtcccagc
      421 ctgaaatgag agcacacgat atcgcaaaaa aataaaaggt attaatacat gatgctacct
      481 tatgacaaat tatatgaata tattcagaaa tatccacata tggttatgcg tgatcatggc
      541 atctatcttc atgacggtgc cggtttcggc aatctacgac ttcgagggca tctccctcga
      601 tacagcggcc cagggcgagg tccgagggga cgtgctcacc ttcggaacct acggtctcgc
      661 cgctcctccc ctcgagtgcg ccttctccct cccggcaaag ccggtctggg cgcgggtcta
      721 cacgggtgtc tggggcggca ccgagcgcta caccgggtgg gaggagctca gcgtcaacaa
      781 cggcgtcccg gtccgccggg acctcttcgg gaaggacgac cggcaccccg agacgctcgt
      841 atcgggatac ggcgtctact gggtcgcgca cgactgcacc gaccagctcc ggggcggcaa
      901 gaacaccgtc accctcacga ccagcagggg ccagcccgag agcaggatcg acgggcgcgt
      961 ctacggcata ttcgtcgtgg ccgtcgtcga ggatgccgca agaccggtca cccggtactg
     1021 gatcgcagaa gggaacgaga acctccacgg cgagggatgg tccggcacga accccacgcg
     1081 ccacgatacg tgcgaagtag tcttcgagaa ggcaggtgca atagagaagg gcgccgccga
     1141 cctgaccgtg gtccttctcg cctcgacgaa agggcagccg gattacatcc tcttcaacgg
     1201 cgaggacctg ggcgttctgg taacggatct ctcgctgtat cccgaagggg cgcgggacat
     1261 cggcaacgaa catatcggcg acgcaaccgg cggtgccggt gtcgaggccc gctacgtcga
     1321 cgtcgagacg ttcgacgtcg gccgcctcct gaaagagacc aaccgcatcg tcttcgagca
     1381 ggggagggac ctcgacggcg acggcgagat cagcaccacc gggtcgaagc ccgagggcga
     1441 ggactacatc cacccggtct ccgcgatcct cacggtgcag cggcccggcg cgtctccggc
     1501 cccggacttc gccgtcgata cacccgtggt gacgggcgct tacgccggcg aggaggcgac
     1561 gatccgggcg atggtgagga actacggcgt acggcccgac gggaagatcg cggtggcctt
     1621 ctcggtcgac gggagtgtcg tcaaaacagt tgaggtcgag ccggcgaggt ccggcgtcca
     1681 gcaggtcgag accggcgtca ccctcgccga aggaaggcat accgtcgcgg tgcgcgtcga
     1741 tgccgccggc gacgccgatc ccgcggacaa cgaggcatcg atgcagctga cggtcggcac
     1801 ggtccccgac ctctccgtcg ccataggggc gccgatccgg ggcggacttg aagccggggc
     1861 acaggccgcg ccgacgcaac aggcgcccgc tccgttcttt gccctgtttg cggggctcg
//
LOCUS       DAOSII010000270         1893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_52717_length_1893_cov_31.646899, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000270 DAOSII010000000
VERSION     DAOSII010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52717_length_1893_cov_31.646899"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="PLV90_11200"
     CDS             <1..671
                     /locus_tag="PLV90_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97723.1"
                     /translation="YQLLRLYEGWEIRGAVVFAVTSDLALNLYAEADASVRSVFRPAE
                     VTFLPYTKAEIRDILADRVRQGLYPGVVPPSVLDLVAEMTAGERDIRVGIALLRAAAV
                     RAEKDGRFRVTRKDVLDVVATVRSPALAARTAALSSGERTLLYRLAEQSLAGGDMMAG
                     AVFEEAQDYLAIGKTTYHARLRRLADAGIVDLLQTGKGYEVFLRYAPGDVFAACAHDD
                     PAKG"
     gene            998..1156
                     /locus_tag="PLV90_11205"
     CDS             998..1156
                     /locus_tag="PLV90_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97724.1"
                     /translation="MSGEDTPDAVPLFLVPQAVADAIRRHGRAVREINVRRTRNHQYS
                     INVERGRS"
     gene            1468..1638
                     /locus_tag="PLV90_11210"
     CDS             1468..1638
                     /locus_tag="PLV90_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97725.1"
                     /translation="MSVAEKIPVSEETYNQILDLRHPGETLDETLARLVEKVKNSGSR
                     MISKRSWHETSS"
BASE COUNT          358 a          609 c          562 g          364 t
ORIGIN      
        1 tctaccagct cctccggctc tacgaggggt gggagatccg gggtgccgtg gtctttgcgg
       61 tcacgagcga tctcgccctg aacctctatg cggaggccga tgcgagcgtc cggtcggtct
      121 tccgcccggc cgaggtcacc ttcctgccct acacgaaggc cgagatccgc gatatcctcg
      181 ccgaccgcgt ccggcagggg ctctaccccg gggtggtgcc gccgtcggtc ctcgatcttg
      241 tcgcggagat gaccgccggc gagcgggaca tccgggtcgg gatcgccctc ctccgggcgg
      301 ctgccgtgcg ggccgagaag gacggccgct ttcgcgtcac ccgaaaagac gtcctcgacg
      361 tcgtcgccac cgtccggtcg ccggcgcttg ccgcccggac cgctgcgctc tcctccggcg
      421 aacgcaccct cctctaccgg ctcgcggaac agtcgctcgc cgggggcgat atgatggcgg
      481 gggcggtctt tgaggaggcg caggactacc ttgccatcgg caagacgacc taccatgccc
      541 ggttacgaag gctcgcggac gccgggatcg tcgacctcct ccagaccggg aagggctacg
      601 aggtcttcct ccgctacgct cccggcgatg tctttgcagc ctgcgcccac gacgacccgg
      661 caaaaggctg aacaggccgc tcccctcagt cttaccgtta attggtgatc cggacttccc
      721 gctacactat tttctcatac gataaagtaa cgtttatatg cagcccttcg cccgattcag
      781 ggcctgtaag gtatcctttt aatcatcggt gtccgagggt gatgtacgcg tccgggagcg
      841 tcccggtgcg tgaggtaacg accatgcaac agagaaaaac cacgagaggc ggcagccagg
      901 gacaaccgaa tgttcacagc accaggatgc accggaccgc caatggtact cggcacccga
      961 acgatataag gatgcccgtc ccgggagcgt gcccgtgatg agcggggaag atacgccgga
     1021 cgcggtcccg ctcttcctcg tcccgcaggc ggtcgcggac gcgatccggc gacacgggag
     1081 agccgtccgg gagatcaacg tcagaaggac ccggaaccac cagtattcaa taaatgtcga
     1141 gcggggccgg tcatgacccc ggggtcgaac cccttcggct tctcccgctc gcacctggcg
     1201 gcgctcacgc tcctgcctcg aagcctgacg gcttctcatg ctccgttttc gcacctgcgg
     1261 cgctcaagct cgctacgctc gctcctcgaa gacctccggt cttctcgaac tcctggccta
     1321 cggacagtcg ttctcctgaa cgtcgcacgt cccggcagtc ctgctccact cctgatggag
     1381 cgtcgcgttc cccctgcctt ctccgctcgc caccctttcc tcccggttga agaaggtata
     1441 tccatgatca gcaccgaagg tgattcaatg tctgttgcgg agaagatccc cgtatcggaa
     1501 gagacctata accagatcct ggatctcagg cacccgggcg agacgctcga cgagactctt
     1561 gcgaggctgg tggagaaggt aaaaaacagc ggctcacgga tgatatcgaa gaggtcatgg
     1621 cacgaaacga gttcgtgaag ttagacctgt gacattccgc ctcatggtgg acaaacgggc
     1681 gcttgcgttc ttaactgctc tcgatgccaa agatcagcgg gttatcaaag aaaagctgaa
     1741 aattttaccc gggatgcatg ttgcggtatg gatatcccga acgaaaggga tagcattgtt
     1801 atgcaatctc atattgcagc ctcatgtcgc accaggggtt tctgaatggt gccagcgaga
     1861 cagtccgttc agccctcgga tgtgccatgg cgc
//
LOCUS       DAOSII010000271         1869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_53684_length_1869_cov_50.465821, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000271 DAOSII010000000
VERSION     DAOSII010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53684_length_1869_cov_50.465821"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PLV90_11215"
     CDS             <1..227
                     /locus_tag="PLV90_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HOF97726.1"
                     /translation="AAFIAGWASLFISATLVAGQLAIAGTFPLDIGLAFMAGYHAVIG
                     LIEGGITAVALYLIASARPDILERSTEVTA"
     gene            227..577
                     /locus_tag="PLV90_11220"
     CDS             227..577
                     /locus_tag="PLV90_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HOF97727.1"
                     /translation="MEKAQFIVTGIAVALVIAAAAPFLASGNPDGLESAFFSIHGAKD
                     FQGNDLDEDAAAAAEEKVVAVTGNDFSYGAPLPDYGIAGMDKPGEALAGVVGTLLVLA
                     LAYGVARVAARPDN"
     gene            606..1439
                     /locus_tag="PLV90_11225"
     CDS             606..1439
                     /locus_tag="PLV90_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF97728.1"
                     /translation="MHLVETRNLTHVYRGDIPALGGVDFIAGRKSRIAVIGPNGAGKS
                     TLFKHFNGILKPTSGEVLVRGEPITRENIREVRKFVGIVFQNPDDQIFSPTVEQDVAF
                     GPTNLGLDEAMVAHRVGEALSLLGIEDLRERVPHHLSGGEKKRVAIAGILAMEPQVVV
                     LDEPTAGLDPRGVADLIAFVNRLPDEYGMTVIFSTHQVDLVAEMADFVYVIDQGKVAA
                     TGTVEEIFDRPELLARTRLDVPVIPRLIRSLQEEGIAIDMAYTYDGAKKSFFDACTRR
                     P"
     gene            1436..>1869
                     /locus_tag="PLV90_11230"
     CDS             1436..>1869
                     /locus_tag="PLV90_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HOF97729.1"
                     /translation="MIDDLYSIEESAYRDSVIHRLDARVKLLVALAGIVAVVAMPYST
                     RIYELGAVLFALSLVLWVFSRLSPAIYLRRLALILPFGIFIIGFQVFVKNPYYEVFHP
                     IAVLPFGIAIYAESVEFASILLVKFIVSISFIILLSSTTKMQ"
BASE COUNT          318 a          624 c          564 g          363 t
ORIGIN      
        1 tcgccgcgtt catcgccggg tgggcgtcgc tcttcatatc cgcgaccctc gttgcgggcc
       61 agctcgcgat cgccggcacg ttcccgctgg atatcggcct cgccttcatg gccgggtatc
      121 atgccgtcat cggcctcatc gagggtggga tcactgcagt tgccctctac ctgatagcat
      181 cggcgcggcc cgacatactt gagcgttcaa cggaggtgac cgcgtaatgg agaaggcgca
      241 gttcatcgtg accggtatag cggtcgccct cgtcattgct gctgccgcac cgttccttgc
      301 gtccgggaac cccgacggcc tcgagagcgc gttcttcagc atccacggcg caaaagactt
      361 ccagggcaac gacctcgatg aggatgccgc tgccgccgca gaggagaagg tggtcgccgt
      421 gaccggcaac gacttctcct acggggctcc cctgcccgac tacggcatcg ccggcatgga
      481 taagcccggc gaagcgcttg ccggcgtcgt cggcacgctc ctcgtactgg ccctggccta
      541 cggcgttgcc cgggtggctg cccgacccga caactaaata cccggacccc tcactttgcg
      601 ccatcatgca cctggtcgag acccggaacc tcacccacgt ctaccgcggc gatattcccg
      661 ccctcggcgg cgtggacttc atcgcaggga ggaagtcccg catcgccgtc atcgggccga
      721 acggggccgg gaagagcacg ctcttcaagc acttcaacgg catactcaaa ccgacgtccg
      781 gcgaggtcct ggtccggggc gaaccgataa cgcgggagaa catccgcgag gtcaggaagt
      841 tcgtagggat cgtctttcag aaccccgacg accagatctt ctcccccacc gtggagcagg
      901 acgtagcgtt cggcccgacc aacctcgggc ttgacgaggc gatggtcgcc caccgtgtcg
      961 gggaggcgct ctccctcctc gggatcgagg acctgcgcga gcgcgtgccg caccacctct
     1021 cgggcggcga gaagaaacgg gtcgccatcg cgggcatcct tgcgatggaa ccgcaggtgg
     1081 ttgtgctcga cgaaccgacc gccgggctcg atccccgggg cgttgccgac ctcatcgcgt
     1141 tcgtaaaccg cctgcccgat gagtacggca tgacggtgat cttctcgact caccaggtcg
     1201 acctcgtcgc ggagatggcg gacttcgtct acgtgataga tcaggggaag gttgcggcca
     1261 ccgggacggt cgaggagatc ttcgaccggc cggagctgct cgcgagaacc cggctcgatg
     1321 tgccggtcat accgagactg atccggtcgc tgcaggagga aggtatcgcc atcgatatgg
     1381 cctacaccta tgacggcgcg aagaagtcgt ttttcgatgc ctgcacgagg agaccatgat
     1441 cgatgacctc tactccatcg aggagtcggc ctaccgcgac agcgtcatcc accggctgga
     1501 tgcacgggtc aaactccttg tcgccctcgc cgggatcgtc gcggtcgtgg cgatgccgta
     1561 ctcgacgagg atctacgagc tcggtgccgt tctctttgcc ctctctctcg tcctctgggt
     1621 attctcacgc ctctcgccgg ccatctacct ccggcggctc gccctcatcc tgccgttcgg
     1681 gatctttatc atcgggtttc aggtcttcgt gaagaacccc tattacgagg ttttccaccc
     1741 gatcgccgtc ctgccgttcg gtatcgcgat atacgcggag tcggtggagt tcgcctcgat
     1801 cctcctcgtg aagttcatcg tcagcatatc gttcatcatc ctgctctcgt cgacgacgaa
     1861 gatgcagga
//
LOCUS       DAOSII010000272         1837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_54944_length_1837_cov_56.157688, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000272 DAOSII010000000
VERSION     DAOSII010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54944_length_1837_cov_56.157688"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..1419
                     /gene="gatB"
                     /locus_tag="PLV90_11235"
     CDS             4..1419
                     /gene="gatB"
                     /locus_tag="PLV90_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOF97730.1"
                     /translation="MKTIVGLEIHVQLATATKLFCGCSTDYRDDGPNTHCCPICLGLP
                     GSLPRLNRKAVEYGLLVAKALDMRVLEESDFARKNYFYPDLAKAYQITQSDRPLAVEG
                     TVEIEDDEGHEKIVRITRVHLEEDPGRLVHVAGASKYSLVDYNRSGIPLLEIVTEPDM
                     RSPQEARRFLNKLRTILEYLGVFDGEREGALRVDANISIEGSARVEVKNISSYKGVEK
                     ALTFEATRQRNLLRRGQTVARETRHFMEGRGITTSARGKEEAHDYRYFPDPDLGPIRV
                     ADRVAAIDLPELPTARKNRFMAQYGISLNHARTLTGDPRLADFYEHVAPADPILAATW
                     AADILLGELNYRNMSIAAVPADRIRELLALLRAETITDAAGVQVLREMLDACAAGRPC
                     EKPADIVKREGLARATGDDFVAIVQAVIASNPQAVEDYRAGKKGALNFIVGQVMKETR
                     GRADPRELGRIVSECIEKGGV"
     gene            1426..>1837
                     /locus_tag="PLV90_11240"
     CDS             1426..>1837
                     /locus_tag="PLV90_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOF97731.1"
                     /translation="MHLIIAEKNISANRIAQILAGSEKVRASKDGSVSTYSFDTKTVV
                     GLKGHVVEVDFEPGYTNWRSEVHTPRSLIDAGIVKKPTEKKIVNLIQKLSKKADLVTI
                     ATDYDTEGELIGKEAYELVRAVNPTVKINRARFSA"
BASE COUNT          398 a          588 c          585 g          266 t
ORIGIN      
        1 ggcatgaaga cgatcgtcgg gcttgagatc cacgtccagc tcgcgacggc gacgaagctc
       61 ttctgcgggt gctcgacgga ctaccgcgac gacgggccca acacccactg ctgcccgatc
      121 tgtctcggtc tgcccgggtc gctcccgcgc ctgaacagaa aggcggtcga gtacggcctc
      181 ctggtagcaa aagccctcga catgcgggtg ctggaagagt cggatttcgc ccggaagaac
      241 tacttctacc ccgatcttgc aaaggcctac cagatcaccc agagcgatag gccgcttgcg
      301 gtcgagggga ccgtcgagat cgaggacgac gaggggcacg agaagatcgt ccggatcacc
      361 cgggtgcacc tcgaggagga cccggggcgc ctcgtccacg tcgcgggcgc atcgaagtac
      421 tcgctcgtcg actacaaccg gtcgggtata ccgctccttg agatcgtcac cgaaccggat
      481 atgcgctccc cgcaggaggc gcgccggttc ctgaacaagc tcaggacgat cctcgagtac
      541 ctgggagtct tcgacggcga gcgggaaggc gctctccgtg ttgacgcaaa catatcgatc
      601 gagggttcgg cgagggtcga ggtcaagaac atatcctcct acaaaggcgt cgagaaggcc
      661 ctcaccttcg aggcgacccg gcagaggaac ctgctccggc gcggccagac agtggcaagg
      721 gagacccggc acttcatgga agggcgcggg atcaccacat ccgcccgcgg caaggaggag
      781 gcgcacgact accgctactt ccccgacccg gatctcgggc cgatccgcgt cgcggaccgg
      841 gtcgcggcga tcgacctccc cgaacttccc accgcgcgga agaaccggtt catggcgcag
      901 tacggcatat cgctaaacca cgcccggaca ctgaccggcg acccgcggct tgccgacttc
      961 tacgagcacg tggcgcccgc cgatccgatc ctcgccgcca cctgggcggc agatatcctc
     1021 cttggggagc tcaactaccg caacatgagc atcgccgccg tcccggccga ccgcatacga
     1081 gaactcctcg ccctcctcag ggcggagacg atcaccgatg cggccggcgt ccaggtcctg
     1141 cgggagatgc tcgatgcctg cgccgcaggc cggccctgcg agaaacccgc cgatatcgtg
     1201 aagcgcgagg ggcttgccag agccacgggg gacgactttg tcgcgatcgt gcaggcggtc
     1261 atcgcctcaa acccgcaggc agtggaggac taccgtgccg gcaagaaggg agccctgaac
     1321 tttatcgtgg ggcaggtgat gaaagagacc cgcgggcggg cggacccgcg agaactcggg
     1381 cgaatcgtat ccgaatgtat cgagaaaggc ggggtgtaat ccgcagtgca cctgatcata
     1441 gcagaaaaaa acatatcagc aaaccggatc gcgcagatcc tcgccggcag cgagaaggtg
     1501 agggcgtcga aggatgggag cgtatcaacc tactccttcg atacgaagac ggttgtgggc
     1561 ctcaaggggc acgtggtgga ggtggacttc gagcccggct acacgaactg gagaagcgag
     1621 gtccacacac cgcgaagcct catcgacgcc ggcatcgtca agaagccgac cgagaagaag
     1681 atcgtcaacc tcatccagaa actctcgaag aaggcggacc tcgtcacgat cgccaccgac
     1741 tacgatacgg aaggcgaact gatcgggaaa gaggcctacg aactcgtccg cgcggtgaac
     1801 cccacggtca agatcaaccg ggcccggttc tcggcaa
//
LOCUS       DAOSII010000273         1780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_57415_length_1780_cov_60.058551, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000273 DAOSII010000000
VERSION     DAOSII010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57415_length_1780_cov_60.058551"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1619)
                     /locus_tag="PLV90_11245"
     CDS             complement(<1..1619)
                     /locus_tag="PLV90_11245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HOF97732.1"
                     /translation="MTADNLSPDEKIAIGLADGDTLTTVSWWGGFYYNIPASSRDLAK
                     ECGIPSADEAAADERREQARRVLFRIAVYCHDKGLEVASNLSRRWSRLPEEIRPTPEE
                     VEHLTKMAKVYQERARRDRNDELLSADQEDLTTGIGLTDIGNSERLIRMYGDDVRYCA
                     TLKAWFVWDGRRWEQDETNRMLDLATRTAKAIFLEAATAQNSETVAKWAIKSGSLVAR
                     RAMIDGAVHIVPVRADEMDARENLFNCLNGTLNLETFEFREHKREDLLTKIAGVRYDP
                     DATCPLWLDHLHTVFAGDEEVITGFQDMMGYSLLQFNPEQLMSILWGSGKNGKSETLK
                     AIALILNDYAVNIESSTLMQSRHDDVGRARPDVLRLKGARFVTCTEPEQDAVLSESLI
                     KSLTGDHAVTARPLYGKPVEFVPGAKIFLATNHLPKVKGTDNGIWRRIWLFPFIAIIP
                     PEKRQPEYGNVLFEQEGPGILNWMIAGLRRYREQGKLTQPAAVRRAMQDYRIASNPVG
                     RFVTEACVVGPREQVGKSDLYDAYLSWCETIG"
BASE COUNT          310 a          584 c          508 g          378 t
ORIGIN      
        1 ccgatggttt cgcaccatga gaggtatgcg tcatagagat cggacttccc gacctgctcc
       61 cggggaccga ccacacaagc ctctgtgacg aatcgcccga ccgggttcga cgcgatccgg
      121 taatcctgca tggcccgccg cacggctgcc ggctgcgtaa gcttaccctg ctcccgatat
      181 cgccggagac ctgcgatcat ccagttgagg ataccggggc cttcctgctc gaacaagacg
      241 ttgccatact ccggctggcg tttttcgggc ggaataatgg caatgaatgg gaatagccag
      301 atccggcgcc agatgccgtt atccgtgcct ttgaccttcg gcaaatgatt cgttgccagg
      361 aagatttttg caccgggcac aaactcgaca ggtttgccat ataacggcct cgcggtaact
      421 gcatggtccc cggtcaggct cttgatcagc gattcggaaa ggacagcgtc ctgctccggt
      481 tcggtgcagg tcacgaaccg cgcacccttg agcctgagaa cgtccgggcg agcgcggccg
      541 acgtcatcgt gccgggactg catcagcgtg ctgctctcga tattcaccgc atagtcgttc
      601 agtatgaggg cgatcgcctt caacgtctcc gacttcccgt ttttacccga accccacagg
      661 atactcatga gctgctcggg gttgaattgc aggagcgagt atcccatcat gtcctgaaac
      721 ccggtgatca cctcctcgtc gccggcgaag accgtgtgca ggtggtcgag ccagagaggg
      781 catgtcgcgt cggggtcata ccgcacaccg gctattttcg taagaaggtc ctcgcgcttg
      841 tgctcccgga actcgaacgt ctccaggttg agggtaccgt tcagacagtt aaacaggttt
      901 tcgcgcgcat ccatctcgtc ggcgcggacg ggaactatat gcacagcgcc gtcaatcatc
      961 gcgcgccgtg ccacgagcga acccgatttg atcgcccact ttgcgacggt ctcgctgttc
     1021 tgtgccgtcg ccgcttcgag gaagatcgcc ttcgccgtcc tcgttgcaag gtcgagcatc
     1081 cggttcgtct cgtcctgctc ccaccgccgg ccgtcccaca cgaaccacgc tttcagcgtc
     1141 gcacaatatc ggacatcatc gccgtacatc ctgatcaggc gttcggagtt ccctatgtcc
     1201 gtcagcccga tcccggtagt caggtcctcc tgatccgcgg acaggagctc atcgtttcga
     1261 tcccttcgcg cacgctcctg gtagaccttc gccattttgg taaggtgctc gacctcctcg
     1321 ggcgtcgggc ggatctcttc cggtagccgg gaccaccgtc tggaaaggtt gcttgcgacc
     1381 tcgagccctt tgtcgtgaca gtagaccgcg atacgaaaca gaacccgcct tgcctgctcc
     1441 cggcgctcgt cggcggccgc ctcgtcagcc gaaggtatcc cgcactcttt cgcgagatcg
     1501 cgcgatgacg cagggatgtt gtagtaaaac ccaccccacc atgacacggt ggtgagggta
     1561 tccccgtctg cgagaccgat cgcgatcttc tcgtccgggg agaggttgtc cgcggtcatg
     1621 ccctcaccgc ctcccgccgg gccgcctgca tcttccgccg gtactcaacg cgcttgaggt
     1681 atcgtgcttc gggcgggagg gcatccatgc cgccgcccgg gctccaggtc tctcccacgt
     1741 gctccagggc cttcgagatc gtccgcgtca cgtagtcggt
//
LOCUS       DAOSII010000274         1753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_58615_length_1753_cov_53.163722, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000274 DAOSII010000000
VERSION     DAOSII010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58615_length_1753_cov_53.163722"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="PLV90_11250"
     CDS             complement(<1..914)
                     /locus_tag="PLV90_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOF97733.1"
                     /translation="MDVEEISRRHLAAGTPDDDIVHLLSRDILAIKHNRISSAYAGAF
                     ARAVLAEAKNSTGLSGDLFEFEPSGSTMGEFGVGSRGSGDFFAHRQLARIIGRTSAAV
                     GVDEMDDAGAVRAGGDYIITTVDGMHSRLSDFPFLAGFHVTRATLRDVYVMGAKPVAL
                     LSDIHVADDGDVAKIFDYTAGVSAVGEAMGVPLVTGSTLRIGGDMVLGTRMTGCVGAV
                     GVANHLTARKAIQPGDVLLMTEGAGGGTIATAAIYSGFPDVVDETINLHFLKACEALL
                     ASDVLNGIHAMTDVTNGGLRGDAYEMAET"
     gene            1011..>1753
                     /gene="thiD"
                     /locus_tag="PLV90_11255"
     CDS             1011..>1753
                     /gene="thiD"
                     /locus_tag="PLV90_11255"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOF97734.1"
                     /translation="MTGAQTITACSIAGSDSGGGAGIQADLKTFTALEVYGLTVVTAV
                     TAQNTREVRGVWMLPADAVRTQIEAVVDGFSIGAWKTGMLGNTSNIRAVAEALPDGAV
                     LVIDPVMVSTSGHRLLDGDAVADLVEQLIPRADIVTPNLPEAEVLGRMRVATLDDMTE
                     AGRRILDLGARAVVVKGGHLAGDAAVDIFIDRDGVVPLSGRRYPYSVHGSGCCFSAAL
                     AAYLARGMAARPAFAAAREFIDTAIREAAG"
BASE COUNT          291 a          539 c          626 g          297 t
ORIGIN      
        1 gtctccgcca tctcgtaggc gtcgccccgg aggccgccgt tcgtgacgtc ggtcatggcg
       61 tggatgccgt tgaggacgtc gcttgcaaga agcgcctcgc aggctttgag gaagtggagg
      121 ttgatcgtct cgtcgacgac atcggggaac cccgagtaga tcgcggcggt ggcgatcgtc
      181 ccgccgccgg ccccctcggt catgaggagg acgtcgccgg gctggatcgc cttcctcgcg
      241 gtgagatggt ttgcgacgcc gacggcaccg acgcaaccgg tcatccgggt tccaagcacc
      301 atgtccccgc cgatccgtaa cgtggatccg gtgacgagag ggacgcccat cgcttcgccg
      361 acggcggaga cgcccgccgt gtagtcgaat atcttcgcga cgtcgccgtc gtcggcgacg
      421 tggatatcgg agaggagggc gacgggtttt gcccccatca cgtagacgtc gcggagcgtc
      481 gcccgggtga cgtggaaccc ggcgaggaag gggaagtcgg agaggcggga gtgcatcccg
      541 tcgacggtgg tgatgatgta gtctcctccg gcacggacgg cgcccgcatc atccatctcg
      601 tcgacgccga ccgcggccga cgtcctgccg atgatccggg cgagctgccg gtgagcgaag
      661 aagtcccccg atcccctcga tccgacaccg aactcaccca tcgtcgagcc ggaaggctcg
      721 aactcaaaga ggtcgccgga gagaccggtc gagttcttcg cctcggcaag gaccgcccgg
      781 gcgaacgccc cggcataggc ggagctgatg cggttgtgct tgatcgcaag gatatcccgg
      841 gaaagaaggt gcacgatatc gtcgtcagga gtgccggcag caaggtgccg gcggctgatc
      901 tcttctacat ccattgtaag agtgatcggt gccgggagag ataaaaggag tggcggcggg
      961 gctctccctg cgggcaaaga ttcaagcgga tccgccccga atacagatgg atgacgggtg
     1021 cgcagacgat caccgcatgc tccatagccg gttccgattc cgggggaggg gcgggcatcc
     1081 aggcggactt gaagacgttc acggcgcttg aggtctatgg gctgacggtc gtaacggcgg
     1141 tgacggccca gaacacccgc gaggtccggg gggtctggat gctccccgcc gatgcggtgc
     1201 ggacacagat cgaggcggtt gtcgacggtt tctccatcgg ggcgtggaag accgggatgc
     1261 tcggaaacac ctcgaacatc cgggccgtcg cggaggccct gccggacggt gccgtgctcg
     1321 tcatcgaccc ggtgatggtc tcgacgtcgg gccaccgtct gcttgacgga gatgcagtcg
     1381 cggatcttgt cgaacagctc atcccccggg cggatatcgt caccccgaac ctcccggagg
     1441 cggaggtgct cggccggatg cgggttgcaa ccctggacga tatgaccgag gccggtcggc
     1501 ggatcctcga cctcggcgca agggccgttg tggtgaaagg ggggcacctt gccggcgatg
     1561 cggcggtcga tatcttcatc gaccgtgacg gggtggtccc gctctccggg cgccgctatc
     1621 cctactcggt gcacgggtcg gggtgttgct tctccgcagc gctcgctgca taccttgccc
     1681 ggggaatggc tgcgcgccct gcgttcgccg cggcccggga gttcatcgat acggccatcc
     1741 gggaggcagc ggg
//
LOCUS       DAOSII010000275         1740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_59263_length_1740_cov_46.538279, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000275 DAOSII010000000
VERSION     DAOSII010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59263_length_1740_cov_46.538279"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(325..>1740)
                     /locus_tag="PLV90_11260"
     CDS             complement(325..>1740)
                     /locus_tag="PLV90_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97735.1"
                     /translation="ELREMYEPKRTPKREEKEPEEKPTPPPADDEHRERALAILRTGD
                     PLAFLLDTFNKSHVGDRTVAECLALSVASQSVENTNGLHVSISGNSGKGKSHACTTML
                     NLIPKDYRLTGTVSDKALYYADDLLPGTVFLFDDMALSDDLQEVLKSATTNFHEPIEH
                     RTLTTERKLRVCSIPERCVWWLAKVENPGDDQVMNRMLTVWIDDSAEQDRRVLEHLKQ
                     VEAGRYRGEDDPDVLTARALWAIVKQHRITVHIPFAGRIGFSSVANRRNPAMLFDLIK
                     CHAMLRFMQREHHEADGRVRIMAAREDFDAAARLYGAINGEGGGQDSKMTKNEAAAIA
                     TIARMGWETFTVRMLQEATGLSYHQVRRILQGYTTRGTTYCGLLEKCPAISMVDATVT
                     DNSAETTIRRHESHFQFDAGRYREWTAELSVWLEEEDPSPPPSGGSGGMPDPCCKGVR
                     NHSRKEIEEGKPQQDKEDLVK"
BASE COUNT          283 a          561 c          520 g          376 t
ORIGIN      
        1 accgcaccgg ccaagctcca ccttcacccg ctcgaacgct cccggagcaa gcaccccggg
       61 cagcggatgt acaacatttg cccccgtgcc gccagggggt gcgcaaacag aagcgcaaac
      121 atgttcagga tataaattct tcaatacgct ggtattttta cgatctacct ttaaataagt
      181 actttcggtc ttttcgttcg cggcaagtcc taaaacacac gacatgcgtc cccggtgctc
      241 ttcccgtgga gtaggtggat ggtgtgttcc tgaatctgcc gctaacaacc cctgatcgcc
      301 gcgatcgatc tctgatgaaa tctctcattt caccagatcc tccttatcct gttgaggttt
      361 cccctcctcg atctctttgc ggctatggtt gcgcaccccc ttgcagcacg ggtccggcat
      421 accgccactc ccgccggaag gcggcgggga ggggtcttcc tcctccagcc agaccgagag
      481 ctctgccgtc cactcccggt agcggccggc atcgaactgg aagtgggatt cgtgccggcg
      541 gatggtcgtc tcggcggagt tatcggtgac cgtcgcatcc accatgctga tggcggggca
      601 cttctccagc agcccgcagt aggtcgtgcc cctcgtcgtg tagccctgca ggatccgccg
      661 gacctggtgg taggagagcc ccgtcgcctc ctgcagcatc cgaaccgtga acgtctccca
      721 gcccatccgg gcgatggtgg ctatggccgc cgcctcgttc ttggtcatct tcgagtcctg
      781 cccgccgccc tccccgttga tcgcgccgta gagccgggcc gcggcatcga agtcttcccg
      841 ggccgccatg atccggaccc tgccgtctgc ctcgtgatgc tcccgttgca tgaaccgcaa
      901 catcgcgtgg cacttgatca ggtcgaagag catggcgggg ttcctgcggt tcgcgacgct
      961 cgagaaccca attcggccgg caaaggggat atggacggtg atccggtgct gcttcacgat
     1021 cgcccagagt gcgcgggcgg tcaggacatc cgggtcgtct tctccccggt acctgccggc
     1081 ctccacctgc ttgaggtgtt cgagcaccct cctgtcctgc tcggccgagt cgtcgatcca
     1141 gaccgtcagc atccggttca tcacctggtc gtcgccgggg ttctcgacct tcgcgagcca
     1201 ccagacgcac cgctcgggta tcgaacagac ccggagtttt ctctcggtcg tgagggtccg
     1261 gtgctcgatc ggttcgtgga agttcgtcgt cgccgatttg aggacctcct ggaggtcatc
     1321 cgagagcgcc atatcgtcaa agaggaagac cgtccccggc aggagatcgt cggcgtagta
     1381 gagcgcctta tcagagacgg tgccggtaag ccggtagtct ttcggaataa ggttgagcat
     1441 cgtcgtgcag gcgtggctct tccctttgcc cgagttgccc gatatcgaga catgaagacc
     1501 gtttgtgttc tcgaccgact gtgaggcgac cgagagggcg agacactcgg caaccgttcg
     1561 gtcgccgacg tggctcttgt tgaaggtgtc gagcaggaag gcgagcgggt cgccggtcct
     1621 gaggatcgca agagcacgct cgcggtgctc gtcgtccgcc ggaggagggg tgggtttctc
     1681 ctccggctct ttctcctctc tcttcggcgt ccgcttcggc tcgtacatct cccggagttc
//
LOCUS       DAOSII010000276         1724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_60054_length_1724_cov_59.430797, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000276 DAOSII010000000
VERSION     DAOSII010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60054_length_1724_cov_59.430797"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="PLV90_11265"
     CDS             complement(<1..1081)
                     /locus_tag="PLV90_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOF97736.1"
                     /translation="MNRVTKYSFIARMPDEPGALHRAADIVKSYSGNINRIQYDRRID
                     PATVFFEVTATPETYVQMKEDLHAIGYLQETLPALGFLKFSLYLPHQPGSLFELLTYI
                     TGAGANIAYIDFDDRRCDPGRVTISLNIEKTAIVESLLDRLKSRYRLDIIEYDMTGEK
                     LDDTVFYVRFAQAVRGLVGTAEDGFLLSFLHDVNHIVQELNSLGQDPKEVFESILATG
                     RTLRNTTGDRFYADLQRIRLSDAVEVFCFQMPCGGNIFLLRAPDETVMIDTGYGIYHE
                     DVMRMFRHYGLPNQQGIGRVYITHADADHCGAGGFSGEKVHTHAGSLAIIRQANRAYG
                     SRSESSILEEVYTTVINLFSRFTPPE"
     gene            complement(1202..>1724)
                     /locus_tag="PLV90_11270"
     CDS             complement(1202..>1724)
                     /locus_tag="PLV90_11270"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOF97737.1"
                     /translation="RDRYIMADRILVAYATRYGSTADVAEAIGDELRKAGETVDVRPF
                     TDIADLSPYRAAVIGSPIYMGKWLPESQVFIEKNQQFLRTIPVAYFAVGLTVKEGTHE
                     FIRKAEASMDQVRLLVQPVEVGVFPGKLETGRLTIADRAIVKLIRAEAGDFRDLAAVR
                     AWAQAVREKIAQA"
BASE COUNT          308 a          507 c          568 g          341 t
ORIGIN      
        1 tctccggcgg ggtgaagcgc gagaagaggt tgatgacggt cgtgtagacc tcctcgagga
       61 tcgaggactc gctgcgcgaa ccgtaggccc ggttcgcctg gcggatgatt gcaagcgacc
      121 cggcatgggt atggactttt tcgcctgaga accctcccgc cccgcagtgg tccgcgtcgg
      181 cgtgggtgat gtagaccctg ccgattccct gctggttcgg gaggccgtaa tgccggaaca
      241 tccgcatgac gtcctcgtgg tagatcccgt atcccgtgtc gatcatgacg gtctcatccg
      301 gggcgcggag gaggaagatg tttccaccgc acggcatctg gaagcagaag acctcgacgg
      361 catcgctgag ccggatacgc tggagatccg cgtagaaccg gtccccggtg gtattccgaa
      421 gcgtccttcc cgtggcgagg atggactcga agacctcttt tgggtcctgg ccgaggctgt
      481 tgagttcctg gacgatatgg ttgacgtcat gcagaaaaga gagcaaaaaa ccatcctccg
      541 ccgtcccgac cagcccccgc accgcctggg cgaacctgac gtagaagacg gtgtcgtcga
      601 gtttctcgcc ggtcatgtcg tactcgatga tatcgagacg gtagcgcgac ttgagacggt
      661 cgagcaggct ctccacgatc gcggtcttct cgatgttcag gctgatggtg accctcccgg
      721 ggtcgcaccg ccggtcgtcg aagtcgatgt aggcgatgtt tgcccccgca ccggtgatgt
      781 aggtgaggag ctcgaagagg gagccgggct ggtggggcag gtagagggag aacttgagga
      841 acccgagcgc cggaagcgtc tcctggaggt agccgatggc gtggaggtct tctttcatct
      901 gtacgtaggt ctccggcgtc gcggtcacct caaagaagac cgtggcagga tcgatccgcc
      961 ggtcgtactg gatgcggttg atgttccccg agtaagactt gacgatatcg gcggcacggt
     1021 ggagcgcgcc cggctcgtcg ggcattctgg cgatgaagga gtacttggtc acccggttca
     1081 cctggagata tcggtactat gctctcgtcc tatatccttc ttccggcaaa aatggtcctg
     1141 attgggggat agggcatacc gggagccccg ttccgggtgt ggacataggg agggcgaccg
     1201 gtcacgcttg cgcgatcttc tcgcgcaccg cctgcgccca ggcacggacg gccgcgagat
     1261 cccggaagtc ccctgcctct gcccggataa gtttgacgat cgcgcgatcc gcgatcgtaa
     1321 ggcggccggt ctcaagcttc ccggggaaga cgccgacctc gacgggctgt acaagcagcc
     1381 ggacctggtc catcgaggcc tccgccttcc ggatgaactc gtgcgtcccc tctttgaccg
     1441 tgagccccac ggcgaagtag gcgaccggga tggtgcggag gaactgctgg ttcttctcga
     1501 tgaagacctg cgactccgga agccatttcc ccatgtatat cgggctcccg atgacggccg
     1561 cacggtaggg ggagaggtcg gcgatatcgg tgaacggccg gacgtcgacc gtctcgcctg
     1621 ccttccggag ttcatcgccg atcgcctccg ccacatccgc ggtcgaaccg tagcgggtgg
     1681 cgtaggcaac aagaatcctg tctgccatga tgtacctatc tctc
//
LOCUS       DAOSII010000277         1692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_61787_length_1692_cov_27.579108, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000277 DAOSII010000000
VERSION     DAOSII010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61787_length_1692_cov_27.579108"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..957)
                     /locus_tag="PLV90_11275"
     CDS             complement(202..957)
                     /locus_tag="PLV90_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOF97738.1"
                     /translation="MAAEIVPGLRGLGMNEYEASVYSTLVGLQKATARDVHEASGVPR
                     GRIYEILNDLARRGFIGVEEGSPASYYILDVDVVFDRLKEDYIRSLEETREALKSLSV
                     KPHLSPDSFFILRSGWAIENHISSLFRRVKKSMVILCYNPEFLQRYYTIIKPLERRID
                     LSIVVRKKEEYAGIKLPIYEARGTVVDLLDSRPVNGPVMEGLRKDECAILVDGRDFFA
                     IATAGSGRHAVIGSDMPIIGYLQKTIVERLREA"
BASE COUNT          378 a          555 c          467 g          292 t
ORIGIN      
        1 cggcacgatc ctctttgcag gcagggtcgt aaacccggtg gggccgtaag gggcgggcgg
       61 gcaacaccgc cccctcctgc agcaacccat ttgacagaat accagcatcc atgcagtccc
      121 acgagatatc ttcgcgaccc tgcaccgtct caagggcgag cttgttggac ggttatcgaa
      181 gactacctcg gcgcgtatct ttcacgcctc ccggagccgt tcgacgatcg tcttctgcag
      241 gtatccgatg atcggcatat ccgaaccgat caccgcgtgc cgcccggacc ccgcggtagc
      301 gatggcgaag aagtcccggc cgtcgaccag gatcgcgcat tcatccttcc gcaatccctc
      361 catcacgggc ccgttcaccg gccgggaatc caggaggtcg acgacggtcc cccgggcctc
      421 gtagatcggg agtttgatcc ccgcatactc ctcctttttc cgcacgacga tagagagatc
      481 gatcctgcgc tcgaggggtt tgatgatcgt atagtatctc tggagaaact cggggttgta
      541 acagaggatc accatgctct tctttacccg gcggaagagc gatgatatat ggttctcgat
      601 cgcccaaccg ctccggagga tgaagaacga gtccggggag aggtgcggtt ttaccgagag
      661 gctctttaac gcctcacgtg tctcttcaag ggatcgaata tagtcttcct tgagccggtc
      721 gaagaccacg tcgacgtcaa gtatgtaata ggaggccgga gacccctctt ccacgccgat
      781 gaacccccgc cgggccagat cgttcaagat ctcgtagatc cgtccccggg gaaccccgct
      841 cgcctcatgg acatcccgtg cagtcgcctt ctgcagcccg acgagcgtcg agtagacgct
      901 cgcctcgtac tcgttcatcc cgagaccccg gagcccgggg acgatctcag cagccattgc
      961 acctgtattg agttgcacca gacttatggt ttccggagaa gactgataga atagcgggag
     1021 agcaaccatc tctcgcaaga ccgtcccccg gagcagccgc ccgccagaag aacctgcagc
     1081 tcatgatatc acccatcaaa ctggcattcc gcatagagca actggaacgc cgggcggggc
     1141 atacccacct tcgaagaagg ctgccccggt aaggacgaat gagagcaaca cgcgcgatac
     1201 cccctgaacc agcagaacct cagcaaagac cgggaagacc cgtcatcgat ataacaccgg
     1261 agcgtatccg ggcgggcagc ggcccggact cccgcctgcc cgacagggtt ctgcaacaaa
     1321 acatctaccc tctcctgaca tcgatcgcag gcgtggcccg gggatgctcc ggacctcaga
     1381 ggccggggcc aaccgccccc ctcatagagg acgatagcct gtccccgccc tggttgcagt
     1441 agattcatat cgaccccctc gaaactacta cactcagcag atggatcatg tatgacgagg
     1501 agagatgtaa actgcgcgcc gacctgcagc accggaccac accagggtcc tggtgaacct
     1561 ccagcacgga tgccgacata ccacagggag ccaaaccccg gagtgggcag caccgggatc
     1621 acccgggagg agcacggatg aagaacccct accaatggct cgccgacgcc atcaacaacc
     1681 acacctgggc cg
//
LOCUS       DAOSII010000278         1682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_62315_length_1682_cov_43.103258, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000278 DAOSII010000000
VERSION     DAOSII010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62315_length_1682_cov_43.103258"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PLV90_11280"
     CDS             <1..435
                     /locus_tag="PLV90_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase subunit beta"
                     /protein_id="HOF97739.1"
                     /translation="GKPTAKKDLDRIVEAHNPPYMATACSAYPLDLYKKVKKALSIPG
                     PKFIHILAPCPPGWRYPTEKTVEMGKLAVKTGTWILYEREYGRLTINGPSKTAMRRPA
                     PLEDYIKGQGRFKNLSPEILDRMRQQIERNVQRLAREEAGAC"
     gene            429..1541
                     /locus_tag="PLV90_11285"
     CDS             429..1541
                     /locus_tag="PLV90_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOF97740.1"
                     /translation="MLTIATGNKAVAAAVKAARPGVVAAYPITPQTEIVEQIAEYVTD
                     GDLESRYIPVESEHSAMAACIGASVAGVRTFTATSSHGLLYMHEMVHWAAGARLPVVM
                     ANVNRALGPGWNTWAEHTDAFSQRDTGWLQVFVGTVQEAYDATLMAFKIAEDERVLLP
                     VMVNLDGFSLSHITQALDTVDIGDFLPPCHLPHAIDTENPLGYGPMTGPADYFRFRWD
                     IERSMRDARGVIAEVEGEFCERFGRSYGPTEDYRCEDADVVVVAMGTLGKEAEVAIDR
                     LRDEGIKAGSMRLRWFRPFPDLDLAGRDVVVIDRDYSFGFGGVVAHSIRSKAGVSPYS
                     VIAGLGGQEVTYNDIADFVRDRRPGEETWFGVNDHV"
     gene            1534..>1682
                     /locus_tag="PLV90_11290"
     CDS             1534..>1682
                     /locus_tag="PLV90_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOF97741.1"
                     /translation="MYEIRIHSRGGQGGVTAAKLLALAAFRDGKYATACPFYGAERRG
                     APVVS"
BASE COUNT          289 a          555 c          578 g          260 t
ORIGIN      
        1 ggcaaaccca cggcaaagaa ggacctcgac cggatcgtcg aggcgcacaa ccctccctac
       61 atggcgaccg cctgcagcgc ctaccccctc gacctctaca agaaggtgaa gaaggcgctc
      121 tcgattccgg ggcccaagtt catccacata ctcgcaccgt gcccgccggg gtggcgctac
      181 cccacggaga agacggtcga gatgggaaaa ctcgccgtga agaccgggac gtggatcctc
      241 tacgagcgcg agtacgggag actcacgatc aacggcccgt cgaagacggc gatgaggcgg
      301 cccgcgccgc tcgaggatta cataaagggc cagggccggt tcaagaacct gagccccgag
      361 atcctcgacc ggatgcgtca gcagatcgag cggaacgtcc agcgtcttgc ccgcgaggag
      421 gccggcgcat gttgacgata gcgaccggca acaaggcggt ggcggccgcg gtgaaggccg
      481 cgcggccggg tgtcgttgcc gcctacccga tcacccccca gaccgagatc gtcgagcaga
      541 tcgccgagta cgtcaccgac ggcgacctcg agagccggta cataccggtg gagagcgagc
      601 actcggccat ggccgcctgt atcggggcga gtgtcgccgg cgtgcggacg ttcacggcga
      661 cgagctcgca cggcctcctc tacatgcacg agatggtcca ctgggcggcg ggtgcgcgcc
      721 tcccggtcgt gatggcgaac gtcaaccgcg ccctcgggcc cgggtggaac acctgggcgg
      781 agcataccga cgccttctcc cagcgcgaca cgggctggct gcaggtcttc gtgggcacgg
      841 tccaggaggc ttacgacgcc accctgatgg cgttcaagat agccgaggac gagcgggtcc
      901 ttcttcccgt tatggtcaac ctggacgggt tctcgctcag ccacataacg caggcgctcg
      961 acacggtgga tatcggcgac ttcctgccgc cctgccacct cccccacgcg atcgacaccg
     1021 agaaccctct cggttacggc cccatgaccg ggccggcgga ctacttccgg ttccgctggg
     1081 acatcgagcg gtcgatgcgc gatgcccggg gcgttatcgc ggaggtcgaa ggggagtttt
     1141 gcgagcggtt cggccgctcc tacggcccca cggaggatta ccggtgcgag gacgccgacg
     1201 tcgtcgtcgt cgccatggga acgctcggga aggaggcgga ggtggcgatc gaccgcctcc
     1261 gcgacgaggg gataaaggcc gggtcgatgc gcctgcgctg gttccgtccc ttccccgacc
     1321 tcgatcttgc cggccgggat gtcgtggtca tcgaccgcga ctactcgttc ggctttgggg
     1381 gcgtggtggc gcattccatc cggtcgaagg ccggcgtttc gccctacagc gtcatcgcag
     1441 ggctcggcgg ccaggaggtc acttacaacg acatcgcgga ctttgtccgg gaccggcgtc
     1501 ccggcgagga gacctggttt ggggtgaacg accatgtatg agatcaggat tcattcccgg
     1561 ggcgggcagg gcggcgtcac cgccgcaaag ctcctcgccc tcgcggcgtt ccgggacggg
     1621 aagtacgcga ccgcctgccc gttctacggg gcggagcggc gcggggcgcc ggtcgtctcg
     1681 tt
//
LOCUS       DAOSII010000279         1607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_66438_length_1607_cov_32.086340, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000279 DAOSII010000000
VERSION     DAOSII010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66438_length_1607_cov_32.086340"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            433..1155
                     /locus_tag="PLV90_11295"
     CDS             433..1155
                     /locus_tag="PLV90_11295"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOF97742.1"
                     /translation="MSVAQDRVEKGCIVPVMIDAQDRAIVRKVSLESDACKGQGCTLN
                     GRGPVFRLPRTVEVAGGSAFVTVQDGDRYISHRITVTGAGETVRIAPRDPAFCGLLVE
                     SNPAGAEILIDGFRTGFCTPYLIRNVSDGPHVVTVTRPGYLPGGEEILLTDDRNADID
                     KHLTFRLEAYPYGSLNVTSEPAGGEIYLYNKNTGEVTPHTFRYMPIGTYEVKVVGNAT
                     FRTADDVTVRPYEVTKRAFDLI"
     gene            complement(1333..>1607)
                     /locus_tag="PLV90_11300"
     CDS             complement(1333..>1607)
                     /locus_tag="PLV90_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97743.1"
                     /translation="AKGRVTVVCADREVSVYSYCGYCRHCAGVRVGKRMIPTPQRQAL
                     SGLRQSANPDENLLNAAIMFNTLVRDGSAIECEDDKGEGFSSMYRR"
BASE COUNT          314 a          522 c          503 g          268 t
ORIGIN      
        1 cggcgaccgc gcgggccagg cggccggttc cccgggattc aaggttgtcc gtatcatcaa
       61 gagcaagaag tatggtcatg gtacgccttt ttccagataa atctgggaga atgagggtaa
      121 aaaggatact gaagccaggc ccatatcaac caggttctgt taatccgatg ccagacaatg
      181 acatcgagtt aaccagcccc tgaagtactg ccggatcggc tctgccggat aaaggcctcg
      241 atcttccaga caactgtgca aaatcctccg tccgggggcg ggggcccgat atatgagacg
      301 gccgcacacc gcccgaggag agacgatgcc gcatcgatct cgcgcccact cccctcgagc
      361 aggcgcgggg catccccatc cgcaagttcc gcgccggtcc cgtatgcata tggtacatac
      421 gattgtggcg ccatgtcggt cgctcaggac cgggtggaga agggctgcat cgtccccgtc
      481 atgatcgatg cgcaggaccg ggccatcgtc cggaaagtat ccctcgagtc cgatgcctgc
      541 aaaggccagg ggtgtaccct gaacggcagg gggccggtct tccgtctgcc ccggacggtc
      601 gaggtggccg gagggagcgc cttcgtcacg gtccaggacg gcgaccgcta catctctcac
      661 cggatcaccg tgaccggggc gggggagacg gtcaggatcg cgccccgtga ccccgctttc
      721 tgcggccttc tcgtcgagtc gaacccggcg ggcgccgaga tcctgatcga cggcttcagg
      781 accggcttct gcacgcccta cctcatcagg aacgtctcgg acgggccgca cgtcgtcacc
      841 gtcacccggc ccggatacct gcccggagga gaggagatcc tgctcaccga cgaccggaac
      901 gccgatatcg acaaacacct cacgttccgc ctcgaggcct acccctacgg cagcctgaac
      961 gtgacgagcg agccggcggg tggagagatc tacctctaca acaagaacac cggcgaggtg
     1021 acgccgcata cgttccggta catgccgatc gggacgtacg aggtgaaggt cgtcgggaac
     1081 gctacgttcc ggacggcaga cgacgtcacc gtccggccgt acgaggtgac caaacgtgcg
     1141 ttcgacctga tctgaccgga acacgcacct atttttcgct cagccttgcg cgggaggggc
     1201 gccggtggac cgttgcgggg caaggaccac cggcgctctt ccgtgggcgg caccccgccc
     1261 gtcctgcgcg aaggaggggg ccctgccgtc gtgccgatag aggcgcggca aaacaaggac
     1321 agaggtcggt attcagcgcc ggtacatcga actgaaccct tcgcccttgt cgtcctcgca
     1381 ctcgatcgcg ctgccgtcac ggacgagggt gttaaacatg atggcggcgt ttagcaggtt
     1441 ctcatccggg tttgcactct gccggagacc ggagagagcc tggcgttgcg gcgtcgggat
     1501 catccgcttt cccacccgga cacccgcaca gtgccggcag taaccgcaat aggagtagac
     1561 cgacacctca cgatctgcgc acacaaccgt cacccggccc ttcgcgt
//
LOCUS       DAOSII010000280         1586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_67713_length_1586_cov_44.499673, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000280 DAOSII010000000
VERSION     DAOSII010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67713_length_1586_cov_44.499673"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1586)
                     /gene="ilvD"
                     /locus_tag="PLV90_11305"
     CDS             complement(<1..>1586)
                     /gene="ilvD"
                     /locus_tag="PLV90_11305"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOF97744.1"
                     /translation="NRALLRSLGVTDREMGQPFIGIANAYNTIVPGHTHLGGLSQKVR
                     EGVAAAGGAAFEFGTIGICDGIAMGHEGMRYSLPSRENIADAVELMVEAHRFDGLVCV
                     GTCDKIVPGMLMAAVRCNIPAIVVTGGPMLPGYAAGRELSLIDIFEGVGRVAAGTMSE
                     EELGELECAAMPGCGSCQGLYTANTMACVTEALGLSLPGCAAIPAVDAAKLRIARESG
                     ERAVDLAREGIRPRDIVSPASLANAIRVDMALGGSSNTVLHLMAIAREAGVPLDLETF
                     NTIGEETPHICHMQPGGPHSMLTLYRAGGIPAVLAMLAGHLDDAPTVSGRSILDIAKT
                     ARVADPDMIRTVKDPVNPAGGLKVVRGTLAPDGAVVKCAAVPAAMWRHQGPARVFDGE
                     TAAMEAILHREIVEGDVVVIRYEGPRGGPGMPEMLAPTSALMGLGYARVALVTDGRFS
                     GGTRGPCIGHVAPEAAVGGPIALLEDGDAVAIDLYAKSLDLLVDPGTLEERRRAWKPP
                     ERRLTGVLARYARTVEQANLG"
BASE COUNT          229 a          555 c          551 g          251 t
ORIGIN      
        1 ccgaggttcg cctgctcgac ggttcgcgcg taccgggcga gaacgcccgt aagccggcgc
       61 tccgggggtt tccaggcccg cctccgctct tccagggttc cgggatcgac caggaggtca
      121 aggctctttg cgtagaggtc gatagcgacc gcatctccgt cctcaaggag ggcgatcggt
      181 ccgccgaccg cggcctcggg ggcgacgtgc ccgatgcacg gtccccgggt gccgcccgag
      241 aagcggccgt cggtcaccag ggcgacccgg gcgtagccga gccccatcag ggcggacgtc
      301 ggggcgagca tctcgggcat ccccggccca ccccggggtc cctcgtagcg gatcacgacg
      361 acgtcgcctt ccactatctc gcggtggagg atcgcctcca tcgccgccgt ctcgccgtca
      421 aagacccggg ccggcccctg atgccgccac atcgccgccg ggacggcggc gcacttgacg
      481 accgcgccgt cgggggcaag cgttccccgc acgaccttca ggccgccggc agggttgacg
      541 gggtctttga cggtcctgat catatccggg tcggcgaccc gggcagtctt cgcgatatcg
      601 aggattgagc ggcccgagac cgtcggggcg tcgtcgaggt ggcccgcaag catggcaagg
      661 accgcaggta tgcctcccgc ccggtagagg gtgagcatcg agtgcgggcc gccgggctgc
      721 atgtggcaga tgtggggggt ctcctcgccg atggtgttga acgtctcaag gtcgaggggg
      781 acgcctgcct cgcgggcgat ggccatcagg tggaggaccg tgttcgacga tcccccgagc
      841 gccatatcca ctcgtatcgc gtttgcaagg cttgccggcg agacgatatc ccgcgggcgg
      901 atgccctccc gtgcgaggtc gactgcccgc tccccgctct cccgggctat gcggagtttt
      961 gccgcgtcga ccgccgggat tgccgcgcaa ccggggaggg agaggcccag cgcctcggtc
     1021 acgcacgcca tggtgtttgc ggtatagagg ccctggcagc tcccgcaccc gggcattgcc
     1081 gcgcactcga gttcgccgag ttcctcctcg ctcatcgtgc cggcggcgac cctgcccacg
     1141 ccctcgaaga tgtcgatcag ggagagttcc cggcctgccg cgtagccggg gagcatcggg
     1201 ccgccggtga cgacgatcgc cggtatgttg caccgtaccg ccgccatgag catgccgggg
     1261 acgatcttgt cgcaggtccc gacgcagacg agaccgtcga accggtgcgc ctcgaccatc
     1321 agctcgacgg cgtcggcgat gttctcccgc gaggggaggg agtagcgcat cccctcgtgg
     1381 cccatggcaa tcccgtcgca gataccgatg gtcccgaact cgaacgccgc cccgcccgcg
     1441 gcggcgacac cctcccgcac cttctgcgag aggccgccaa ggtgggtgtg cccggggacg
     1501 atcgtgttat acgcgtttgc tataccgatg aagggctgac ccatctcgcg gtcggtcacg
     1561 ccgagcgacc tgagcagcgc ccggtt
//
LOCUS       DAOSII010000281         1585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_67771_length_1585_cov_37.735948, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000281 DAOSII010000000
VERSION     DAOSII010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67771_length_1585_cov_37.735948"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="PLV90_11310"
     CDS             <1..1063
                     /locus_tag="PLV90_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF97745.1"
                     /translation="SIRAIMLSDLAGLMAGIDSGAARGYLGEAQGMLESVGPEHEAFV
                     RKNLIYAARSLNAINRQEKDVDWAVEQVGRIEDPVEYVDALAAVFDMVGEPAQRKEIL
                     AAMCHTVVSIPSSYIRLSMLFDVAKFAEAYGEEEEIDGLLDGMERTAGYIQIPFITAM
                     TRQRMAQMLFSFYRKSGKPAIRQRAVEIVSAIDDDRIRYNLMVQLEQAMPQSWKSTVY
                     SKILDCRDRIRSGDYATKDMVTLDRAIRAATDRAKRATYYTEVYLIARNAGQHEVADR
                     MLFCALEEARIIRPLSRRAFALGDMACRIYAERYEDRSREILNMAVGEALNIRDSALR
                     DEVYDELDMSMRIIQEHWL"
     gene            1060..1347
                     /locus_tag="PLV90_11315"
     CDS             1060..1347
                     /locus_tag="PLV90_11315"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOF97746.1"
                     /translation="MKTIEIRIAGRVQGVGFRACVKRIATNLGVGGAVMNLPDGRVLI
                     TATGEPVILDKFVSMLYGCPRVVIRDLSEREIPNAAYPEFTIQRSAYQYST"
     gene            complement(1329..>1585)
                     /locus_tag="PLV90_11320"
     CDS             complement(1329..>1585)
                     /locus_tag="PLV90_11320"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HOF97747.1"
                     /translation="SLVHDLIEPQKAVMVDRTVIRFAREEIAEGDYECSDKRCYLEAD
                     VSTRLITALRDSIDQSRIDTQVRILRDALLANADFHVLYW"
BASE COUNT          325 a          482 c          505 g          273 t
ORIGIN      
        1 atccatccgt gcgatcatgc tctccgacct tgccggcctc atggcaggga tcgattccgg
       61 cgcagccagg ggctacctcg gcgaggccca ggggatgctt gagtccgtcg ggccggaaca
      121 cgaggcgttc gtcaggaaga acctcatcta cgctgcacgg agcctcaacg ccatcaaccg
      181 tcaggagaag gacgtcgact gggcggtgga gcaggtcggc aggatcgagg atccggtcga
      241 gtacgtcgat gcgctcgcag ccgtcttcga tatggtcggc gaaccggcac agaggaagga
      301 gatcctcgcg gccatgtgtc acaccgtggt gagcatcccc tcctcctaca tcaggctctc
      361 gatgctcttt gatgtggcga agttcgcaga ggcctacggc gaagaggagg agatcgacgg
      421 gcttctggac ggcatggaga gaacagccgg gtacatccag atcccgttca tcaccgccat
      481 gacacgccag cggatggccc agatgctctt ctcgttctac cggaaaagcg ggaagccggc
      541 catccggcaa cgtgccgtcg agatcgtctc cgccatcgac gacgaccgga tccggtacaa
      601 cctgatggtc cagcttgagc aggcgatgcc ccaatcctgg aagagcaccg tctacagtaa
      661 gatcctcgac tgccgcgaca ggatccggag cggcgactac gccacaaagg atatggtcac
      721 cctcgaccgg gcgatacggg cggcaaccga ccgggcaaag cgcgcgacct actacaccga
      781 ggtctacctc atcgcgagga acgcggggca gcacgaggtt gcggacagga tgctcttctg
      841 cgcgcttgag gaggcgcgga tcatcaggcc gctctcgcgg cgggcctttg ccctcggcga
      901 tatggcgtgc cggatctacg ccgagcgcta cgaagaccgg tcaagggaga tcctcaatat
      961 ggccgtgggc gaggcgctca acatacgcga ctcggcgctc cgggacgagg tatacgacga
     1021 gctcgatatg tccatgcgga tcatccagga gcactggctg tgaagacgat cgagatcagg
     1081 atagcgggaa gggtacaggg tgtcgggttt cgcgcatgcg tcaagaggat cgccaccaac
     1141 ctcggcgtcg gcggcgcggt gatgaacctc cccgacgggc gggtcctcat cacggcaacc
     1201 ggcgaacccg tcatactcga caagttcgtc tccatgctgt acgggtgccc ccgggtggtc
     1261 ataagggatc tctcagagcg ggagatccca aacgccgcct accccgagtt cacgattcag
     1321 cggagcgcct accagtatag cacgtgaaaa tcggcgttcg caagaagtgc atcccgcagg
     1381 atacgcacct gcgtgtcgat gcgggattgg tcgatagagt cgcggagcgc agtaatcagc
     1441 cgggttgaga cgtcggcctc aaggtaacat ctcttatcgc tgcactcgta gtctccctct
     1501 gcgatctcct cgcgggcaaa cctgatgacg gtgcggtcca ccatcaccgc tttctgcggt
     1561 tcgatgaggt cgtggacgag gctcc
//
LOCUS       DAOSII010000282         1571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_68642_length_1571_cov_49.629947, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000282 DAOSII010000000
VERSION     DAOSII010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68642_length_1571_cov_49.629947"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..1278
                     /locus_tag="PLV90_11325"
     CDS             175..1278
                     /locus_tag="PLV90_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOF97748.1"
                     /translation="MADVYFANLRARSHHESKIQKIRRLFDAAGFDRVVRPGDLTAVK
                     LHVGERGCDTYINPIFARQVVDKVKERRAHPFITDTGTLYAGSRADAVRHTVTAIEHG
                     FDYAVVGAPVIIADGISGGYWREVAVGGNHFENVRIAGGILDAESMLVLSHVKGHDLA
                     GFGGAIKNLAMGCAPPSGKAEQHAGRPCFETETCKGCGRCVQVCPQAAVTLVEGRARF
                     NPERCVGCGDCMRSCGEGAIEFDWTTEIRPFIERLCEYALGAVRDKPGRVGYINFLLA
                     ITPDCDCVPWSDAAIVPDIGILASTDPVAIDHASFDLVNSERGIARTALGEHTAPGED
                     KFRGIWDYTDGGYQIDYAARIGLGDASYRLIEI"
     gene            complement(1332..>1571)
                     /locus_tag="PLV90_11330"
     CDS             complement(1332..>1571)
                     /locus_tag="PLV90_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOF97749.1"
                     /translation="CGPCRMVAPVIEDLARDFDGRVTFGKCNVDQNRAISTQFSITAI
                     PTLLFFANGMLIDRVVGALPKEAVRARVMRAFPTG"
BASE COUNT          279 a          508 c          532 g          252 t
ORIGIN      
        1 cgctccgggt cagcgagtcg accgggacct cggtcacggg tctgctcggc gacggggggc
       61 cgacgatcac cgccaccaca tcgaacggcg gtatcgacct ctcgggcctc tgaccggcaa
      121 gcacaaccct tatcccctgc cgatccgaga gtacccatac gggagcagac gacgatggca
      181 gacgtctatt tcgcgaatct ccgggcgagg agccaccacg agagcaagat acagaagatc
      241 cgccgcctct ttgatgcggc cgggtttgac agggtggttc gcccgggcga ccttacggcc
      301 gtcaaactcc acgtcgggga acgggggtgc gacacctaca taaacccgat ctttgcccgg
      361 caggtggtcg ataaggtgaa ggagcgccgg gcccacccgt tcatcaccga taccggcacc
      421 ctctatgcgg ggagccgggc cgacgccgtc cggcacaccg tcaccgcgat cgagcacggg
      481 tttgattacg cagtcgtcgg cgctccggtg atcatcgccg acggcatatc gggcggctac
      541 tggcgtgagg tcgcggtcgg cgggaaccat ttcgagaacg tcaggatcgc cggcggcatc
      601 ctcgatgccg agagcatgct cgtcctctcg cacgtcaaag gccacgacct cgccgggttt
      661 gggggcgcta tcaagaacct ggcgatgggc tgcgccccgc cctcggggaa ggcggagcag
      721 cacgccggcc ggccgtgctt cgagactgag acctgcaagg ggtgcgggag gtgcgtccag
      781 gtctgcccgc aggcggcggt gaccctggtc gaggggcggg cgcggttcaa cccggagcgc
      841 tgcgtcgggt gcggcgactg catgcgctcc tgcggagagg gggcgatcga gtttgactgg
      901 acgacggaga tccggccgtt catcgagagg ctctgcgagt atgccctcgg cgccgtccgc
      961 gacaaacccg gaagggtggg ctatatcaac ttcctccttg cgatcacccc cgactgcgac
     1021 tgcgtcccct ggagcgatgc ggcaatcgtc cccgatatcg ggatcctcgc ctccaccgat
     1081 ccggtcgcga tcgaccacgc gagcttcgac ctcgtcaaca gcgagcgggg gattgcccgg
     1141 acggctctcg gggagcatac cgccccgggg gaagacaagt tcaggggaat ctgggactac
     1201 acggacggcg gttaccagat cgactacgca gctcggatag gacttggcga cgcatcctac
     1261 cggctgatcg agatctgaga gggaggtgtg atctgcgggt ctcgcgcccg gtgagaggac
     1321 ccgggcatcc cctaaccggt cgggaacgcc cgcatcaccc gtgccctgac ggcctctttc
     1381 gggagcgcgc cgaccacccg gtcgatgagc atgccgtttg caaagaacag gagcgtcggt
     1441 atggccgtta tgctgaactg tgtcgatatc gcgcggttct ggtcgacgtt gcacttgccg
     1501 aaggtcaccc ggccgtcaaa gtcacgcgca aggtcctcga tcaccggggc caccatccgg
     1561 caggggccgc a
//
LOCUS       DAOSII010000283         1535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_70966_length_1535_cov_62.813514, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000283 DAOSII010000000
VERSION     DAOSII010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70966_length_1535_cov_62.813514"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1535
                     /locus_tag="PLV90_11335"
     CDS             <1..>1535
                     /locus_tag="PLV90_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOF97750.1"
                     /translation="LGGKLVELLEDISIRVLPVTDDDIRTMVREIEGYRLIRGYRGEP
                     QKDEEALVRIIATMARQFAENPQIREFDLNPVILYEEGASVVDARIIVGDTVGGEAAR
                     LSVGSPPDIFYPRSIAVIGASASPNKVGYSVLRNLLSFPGNLYPVNPSRKELFGRTAY
                     PSVLDIPGPVDWAVIAVPAPLVPGVMEECGKKGVRLAIIVTAGFREIGGAGVALEEEV
                     VEIARRHSVRIIGPNCLGVMMPHQGINATFDPVSPRSGDVAFISQSGAIITTVVDWSL
                     PEEFGFSSVISVGNQADLGFEHYLRFAEQDPMTRSITLYVEEIQDGRSFAQIVREVAR
                     RKPVVAVKSGSSRKGRAAASSHTGSLAGSYDVYVAAFRQAGVIPARTLRDAFNLAELL
                     ASEGYPRGNRAIAVTSAGGFAVLASDYAEAHGVDMVDLPDEVLRELNAFLPPFWNRAN
                     PMDIIGDADAARFAALFDVLIRHQDFWDIAFVIAVPTTLADPAHVANEIVRFSRNTEK
                     MVVG"
BASE COUNT          262 a          518 c          490 g          265 t
ORIGIN      
        1 cttggcggga agctcgtgga actcctcgag gatatctcca tccgtgtcct gcccgtcacc
       61 gacgacgata tccgcacgat ggtccgggag atcgagggct accgtctcat caggggatac
      121 cggggggagc cccaaaagga cgaggaggcg ctcgtccgga tcatcgcgac gatggcccgg
      181 cagtttgccg agaacccgca gatccgggag ttcgacctca acccggtcat cctctacgag
      241 gagggagcga gcgtcgtcga tgcgcggatc atcgtcgggg ataccgtcgg aggcgaagca
      301 gcccgcctca gcgtcggatc accccccgat atcttctatc cccgatccat cgccgtgatc
      361 ggcgcatccg caagcccgaa caaggtgggc tactccgtcc tcagaaacct gctttctttc
      421 ccgggaaatc tctatccggt caacccgtcc cggaaagagc tcttcgggcg gacggcctac
      481 ccctccgttc tggatatccc cggaccggtc gattgggcgg tcatcgccgt gccggccccg
      541 cttgtccccg gggtgatgga ggagtgcggg aagaaaggcg tccggctcgc catcatcgtc
      601 accgccgggt tccgcgagat cggcggcgcc ggcgtggccc tcgaggaaga ggtcgtcgag
      661 attgcacgcc ggcattccgt ccggatcatc ggcccgaact gcctcggcgt catgatgccc
      721 caccagggga tcaacgccac gttcgacccg gtctcgccga gatccgggga cgtggccttc
      781 atctcccaga gcggcgcgat catcaccacc gtcgtcgact ggagcctccc ggaggagttc
      841 gggttctcaa gcgtcatcag cgtcggcaac caggcggacc tcgggttcga acactacctc
      901 cggttcgccg agcaggaccc gatgacccgg tcgatcaccc tctacgtcga ggagatccag
      961 gacgggcgca gcttcgcgca gatcgtccgc gaggtcgcca ggaggaaacc ggtggtagcg
     1021 gtgaaatcgg ggtcatcacg gaagggcagg gctgcggcct catcccacac cggttctctt
     1081 gccgggagtt acgatgtcta cgtcgcggcg ttcaggcagg cgggggtgat cccggcccgg
     1141 accctccgcg acgccttcaa tctcgccgaa ctcctggcaa gcgagggcta cccgcggggg
     1201 aaccgggcga ttgccgtcac cagcgcgggc gggtttgccg ttcttgcctc cgactacgcc
     1261 gaggcgcacg gtgtcgatat ggtcgacctc cccgacgagg tgctccgcga actcaacgcg
     1321 ttccttcccc cgttctggaa ccgtgcaaac ccgatggata tcatcgggga tgccgacgcc
     1381 gcccggttcg ccgccctctt cgatgtgctg atccggcacc aggacttctg ggacatcgcg
     1441 ttcgtcatcg ccgtcccgac gacgctcgcc gacccggcgc acgtcgcaaa cgagatcgtc
     1501 cggttctccc ggaatacgga gaagatggtg gtggg
//
LOCUS       DAOSII010000284         1530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_71317_length_1530_cov_40.175593, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000284 DAOSII010000000
VERSION     DAOSII010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71317_length_1530_cov_40.175593"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..673
                     /locus_tag="PLV90_11340"
     CDS             80..673
                     /locus_tag="PLV90_11340"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOF97751.1"
                     /translation="MILSPAFYERDTVTVAKDLLGCLLVHQNGEGTAMGWIVEAEAYL
                     CGDPAAHSFRGETARNRTMFGPAGHAYVYRIYGLHTCINVVTGPEGIGEAVLIRALEP
                     AAGLDLMRARRGTDEALALASGPGKLTQALGITMDLDGASLSGGPLRVLSPESLPGRR
                     PEGWPGEVVRTTRVGITKAADLPLRFYIKGSRYISRR"
     gene            complement(655..>1530)
                     /locus_tag="PLV90_11345"
     CDS             complement(655..>1530)
                     /locus_tag="PLV90_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOF97752.1"
                     /translation="MKRKIIDIDQEKCTGCGLCIPDCPEGALQIIEGKARLVSDLFCD
                     GLGACIGTCPEGAISVVEREAEPYDETAVMAEKIAPQGEAVIRAHLEHLLGHGEAGLY
                     REAIEYLTAHGIPVPAHETAPAAPAACPGTVSRSILREEAAEQAARVEPELRQWPVQL
                     TLVNPAAAYFDDADLLISGDCVPFAYPEFHRDFLRGKILLIFCPKLDADVAGYITKLA
                     DIFSRHTIRSITVLHMEVPCCSGVRFVVDRALERAGKTIPVNEQMILISGRVAEEGER
                     TVQRRGSGMGHRRDM"
BASE COUNT          236 a          518 c          515 g          261 t
ORIGIN      
        1 ttttctttcc cggcgggtgt accgccatcg cccggaacct tgatggggga agggaagaga
       61 ccttgcaggg gagtacgcca tgatcctttc gcccgcgttc tacgaacggg ataccgtcac
      121 cgtcgcaaaa gacctgctgg gatgcctgct cgtccaccag aacggagagg ggacggcgat
      181 gggatggatc gtcgaggccg aggcctacct ctgcggcgac ccggcggccc actcgttccg
      241 gggggagacg gcaaggaacc ggacgatgtt cgggccggcg ggccatgcct acgtctaccg
      301 gatatacggc ctgcacacct gcatcaacgt cgtgaccggc ccggagggca tcggggaggc
      361 ggtcctcatc cgggcgctcg aaccggcggc cggcctcgac ctgatgcggg cgcggcgggg
      421 gacggatgag gcgctcgcgc ttgcaagcgg cccgggcaaa ctgacgcagg cgctcgggat
      481 cacgatggac ctcgacgggg cctccctctc cggcggcccc ctccgggtcc tgtcgcccga
      541 gagcctcccc ggccgccggc cggaggggtg gccgggcgag gtcgtccgga cgacccgggt
      601 cggtataacg aaggcggcgg atcttcccct ccggttctac atcaagggga gccgctacat
      661 atcgcggcgg tgacccatcc cggatccccg gcgctggacc gtcctctcgc cctcctctgc
      721 cacccggccg gatatcagta tcatctgctc gttcaccggt atcgtctttc ctgcacgctc
      781 gagggcccgg tccacgacga accgcacgcc gctgcagcag ggcacctcca tgtggaggac
      841 cgtgatcgac cggatcgtgt gccgcgagaa tatatccgca agtttcgtga tgtagccggc
      901 gacatccgca tccagtttcg ggcagaatat gagcaggatc ttcccccgca ggaagtcgcg
      961 gtggaactcc gggtaggcga aggggacgca gtcgccggat atgaggaggt ctgcgtcgtc
     1021 gaagtaagca gccgcgggat tgacgagcgt caactggacc ggccactgcc ggagctccgg
     1081 ctccacccgt gcggcctgct ctgcagcctc ttccctgagg atgctccttg acacggtgcc
     1141 cgggcacgcc gccggggcgg cgggagcggt ctcgtgcgca gggaccggta tgccgtgcgc
     1201 cgtcaggtac tcgatcgcct cgcggtagag ccccgcctcc ccgtggccga gaaggtgctc
     1261 gaggtgcgcc ctgatgaccg cttctccctg gggagcgatc ttctccgcca tcacggccgt
     1321 ctcgtcgtat ggttcggcct cccgctcgac gacggagatc gccccctcgg ggcatgtccc
     1381 gatgcaggcg ccgagcccgt cgcagaagag gtcgctcacg agccttgcct tcccctcgat
     1441 gatctggagc gctccctcgg ggcagtcggg tatgcagagc ccgcacccgg tgcacttctc
     1501 ctggtcgata tcgatgatct ttcgcttcat
//
LOCUS       DAOSII010000285         1510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanoculleus sp. isolate AMR_MDS_1705
            NODE_72736_length_1510_cov_41.824055, whole genome shotgun
            sequence.
ACCESSION   DAOSII010000285 DAOSII010000000
VERSION     DAOSII010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863234
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoculleus sp. (wastewater metagenome)
  ORGANISM  Methanoculleus sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:55:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,270
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Methanoculleus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72736_length_1510_cov_41.824055"
                     /isolate="AMR_MDS_1705"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:90427"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(314..1423)
                     /locus_tag="PLV90_11350"
     CDS             complement(314..1423)
                     /locus_tag="PLV90_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HOF97753.1"
                     /translation="MKVSPYIPEPLPPAGIDWESYIPQIASANRALARYDGILQAIPN
                     PRLLLSPLLTQEAVLSSRIEGTQASLEDVLRFEANPREPIGDAALADIREIINYREAL
                     NVAVEALKARRLDTALICDLHRILLSGSRGMDREPGCVRTIQNFIGRDARIEHAIFVP
                     PAPEDLPRALADWEAYLQQGELDILVQLSILKAQFELIHPFCDGNGRIGRMLVPLIMY
                     EKGLIGSPMFYISAYLERNRPAYYERLLAVSRDRDWNGWIAFFLHAIEEQAGTNGQKA
                     KAILDLYDEMKRTVPEATRSQYAIAAIDALFGAPIFSSAEFYERSAIPKVSGAKILKD
                     LEGNDVVEILQRGAGRRASTYAFPRLLAITEGNRL"
BASE COUNT          260 a          474 c          471 g          305 t
ORIGIN      
        1 gaaggcccca ggaaacctga tgaggaatgc acgccgggcc gccttatctc tgcggagccc
       61 gcggccttcc cgcttcgccc cgatggcccg gcggtcggcc gggtccgatc gttgtgcgtc
      121 gccggcgtcg cataaaattg gttgtgagac accaaagggc gcttcatggc ccgcaaaatg
      181 tgttgtggct cataggtgtc ccgcaaccgc ctccgaggta tggtttgtgc ataccggagt
      241 atatacaaga tctgttgtgc atatcaaatc caggttttga tatacacaaa tgggtttgtt
      301 ggtaccagaa tactcacaac cggttcccct cggtgatggc gagcaggcgc gggaaggcat
      361 acgtgctcgc ccttcgcccc gcccccctct gcaatatctc tacgacgtcg tttccctcca
      421 gatccttgag gatctttgcc ccacttacct tcgggattgc cgaccgttcg taaaattcgg
      481 ccgagctgaa tatcggcgcc ccgaagagcg catctatcgc ggcgatggcg tactgcgacc
      541 gggtcgcttc cggcaccgtc cgcttcatct cgtcatagag atcgaggatc gcctttgctt
      601 tctggccgtt cgttcccgcc tgctcctcga tggcgtggag gaagaacgct atccagccgt
      661 tccagtcccg gtcgcgggag accgcgagca gccgctcgta gtaggccggc cggttccgct
      721 caaggtaggc gctgatgtag aacatcgggc ttcctatcag ccccttctcg tacatgatca
      781 gcggcacgag catccgcccg atacgcccgt tgccgtcaca gaacgggtgg atgagttcaa
      841 actgcgcctt taagatggag agctggacga ggatatccaa ctccccctgt tgcaggtatg
      901 cttcccagtc cgcgagcgcc cggggaagat cctccggcgc cggcgggaca aagatcgcgt
      961 gctcgatccg ggcgtcccgc ccgatgaagt tctggatcgt gcggacgcac cccggttccc
     1021 ggtccatccc ccggctgccg gaaaggagga tccggtgcag gtcgcagatg agggcggtgt
     1081 ccagccgccg ggccttgagg gcctccaccg caacgttgag cgcttcccgg tagttgatga
     1141 tctcccggat atcggcaagg gccgcatcgc cgatcggctc cctcgggttc gcctcaaagc
     1201 gcaggacatc ctcaagcgac gcctgcgtcc cctcgatccg tgaggagagc accgcctcct
     1261 gggtcaggag cggagagagc aggaggcgtg ggtttggtat cgcctggagg atgccgtcgt
     1321 accgggcgag ggcgcggttt gccgatgcaa tctgcgggat atagctctcc cagtcgattc
     1381 cggccggggg caggggttcc gggatgtagg gggagacctt catgctgccc cctcccggct
     1441 cgccgtgatc ctgccgggac cagggagccc ggcgacgtgc cggagttcct tcatatacta
     1501 cctgatggtg
//
